LOCUS       DASRKH010000001         2113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_40082, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000001 DASRKH010000000
VERSION     DASRKH010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40082"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="VFC06_00005"
     CDS             <1..810
                     /locus_tag="VFC06_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016664714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HZL80319.1"
                     /translation="NFLAHIMGSDKFRQYVEASQVSGNYPSISDARVRAYRIPVPPLE
                     VQREIVRILDQFTQLEAELEAELEARRHQYEHYRGGLLKFGVDVGVRPLGELGENLDS
                     RRRPVTRGARTPGEIPYYGASGVVDFVSEYIFDGDYLLVSEDGANLLARSTPIAFSIS
                     GKTWVNNHAHILQFETYAERRFVEIYLNSIDLSPFISGAAQPKLNKANLNRIPIPDPT
                     LDEKKRVVELLDKFDALVTDLSIGLPAELAARRKQYEYYRDKLLTFKELAA"
     gene            850..1428
                     /locus_tag="VFC06_00010"
     CDS             850..1428
                     /locus_tag="VFC06_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010534589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HZL80320.1"
                     /translation="MRTEAPLLAPVFRSDGQARLLAELLLTGDELSITDLAKRAHLAY
                     PTAWDEVQRLRSAGILVDRAVGRTKLIRANHQSPLVAPLRQILLVATGPAILLAEELA
                     QIPGITSAFLYGSFAARAQGVDGPPPSDIDLMVVGSPKVEQIYSACDRVSDAVGRPVN
                     PTILSEEEFAEDSGFLRSVRESPTVSVVGSAL"
     gene            1628..>2113
                     /locus_tag="VFC06_00015"
     CDS             1628..>2113
                     /locus_tag="VFC06_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZL80321.1"
                     /translation="MAPMISRHIEPRVFEALSDTRVVVIQGARQVGKSTLLNAVVRQA
                     GGRTVTLDDQPTRSAAQTDPVGFLEQGGDGVLGVDEIQRVPALVLALKLIVDRDPRPG
                     RFLVTGSANLLRVPAAEDSLAGRAESLELHSFSQGERDGHLERFIDRLFAGDRFLDHR
                     ST"
BASE COUNT          405 a          691 c          622 g          395 t
ORIGIN      
        1 aacttcctcg cgcacattat gggttccgac aagttccgtc agtacgtcga ggcaagtcag
       61 gtatcgggga attatccgtc gatctctgac gcccgcgtgc gcgcttaccg gatccccgtg
      121 ccgcctctcg aggtgcagcg ggagatcgtg cgaatactgg atcagttcac tcagctggaa
      181 gcggagctgg aagcggagct ggaagcacgc cgccaccagt acgagcacta ccggggcggg
      241 ctcctgaagt tcggcgtgga cgttggggtg cgtccgctcg gtgagcttgg tgaaaatctc
      301 gacagtcgcc gacggccggt cacgcgaggg gcgcggacgc ccggtgagat tccttactac
      361 ggagcttccg gcgtcgtcga cttcgtgagc gagtacatct tcgatggtga ttatctgctc
      421 gtgtcggagg acggcgccaa tcttctcgca cgctcgacac cgatcgcatt ctcgatctcc
      481 ggtaagacct gggtcaacaa ccacgcccac atccttcagt tcgagaccta cgccgagcgt
      541 cgctttgttg agatttacct gaactccatc gatctctcac cattcatttc aggggcggcc
      601 cagccgaagt tgaacaaggc aaatctgaat cggatcccga tcccggaccc gactcttgat
      661 gagaagaaac gcgtcgtcga gttacttgac aagtttgatg cgcttgtgac cgacctgagc
      721 attggtctgc ctgccgagct cgccgcacgt cgcaaacagt acgagtacta ccgcgacaag
      781 ctgctcacct tcaaggagct ggcggcatga tcattgacta tcggtataac cgagaatatt
      841 atcggttata tgagaactga agcgcccctg ctcgcaccgg tctttcgttc ggacggacaa
      901 gcgcgcctgc tcgccgaact gctgctcaca ggagacgagt tgagcatcac ggatctcgcc
      961 aaacgcgcac atctcgccta tcccactgcg tgggatgagg tgcaacgact gcgtagtgct
     1021 gggatcctcg tcgaccgcgc agttggccgc accaagctga tccgagccaa ccaccaaagc
     1081 cctctcgtcg cgccgctgcg ccagatcctg ctcgtcgcca cgggacccgc gatccttcta
     1141 gccgaggaac tcgctcagat tcccgggatc acctcggcct tcctctacgg ctcgtttgcc
     1201 gccagggcgc aaggagtcga tggcccgccg cccagcgaca ttgacctgat ggtggtggga
     1261 tcccccaaag tggagcagat ctacagcgca tgcgatcgcg tgagcgacgc ggtcggccgg
     1321 cccgtcaatc ccacgattct cagtgaagaa gagttcgcgg aggactcggg attcctccga
     1381 agtgtccgcg agagcccgac cgtgtcggtc gtcgggagcg cactgtgaac ttgtctcctc
     1441 tcacaccaca gcagcaggcg gcagagtcat gggtggcgac cggcagcgaa caatccaccg
     1501 gatgtgaggc agttgcccca cggtcgactt ggggagagct caaggtgctt gcgaatccac
     1561 cagcaggtgt ctgcgaatcc accgcttcac cattgcgaat ccaccaatcg ggccctagcc
     1621 ttggctcatg gccccgatga tctcccgtca catcgagccg cgcgtgttcg aggcactctc
     1681 agacacccgc gtcgtcgtga tccaaggcgc ccggcaagtc ggcaagtcca ccctgctcaa
     1741 cgccgtggtg cgccaagccg gcgggcgcac ggtcaccctc gacgaccagc ccacccgcag
     1801 cgccgcgcag accgacccgg tgggtttcct cgaacaaggt ggcgatggag tccttggcgt
     1861 cgatgagata cagcgggtgc ccgccctggt gctcgcgctc aaactcatcg tcgatcgaga
     1921 cccgcgcccg gggcgctttt tggtcaccgg ctccgccaac ctcctccgcg tgcccgccgc
     1981 ggaggacagt cttgcgggac gcgccgaaag tctcgaactc cacagcttca gtcaaggcga
     2041 acgcgacggg cacctcgagc gcttcatcga ccgcctgttc gcaggcgacc gctttctcga
     2101 ccaccggagc acg
//
LOCUS       DASRKH010000002         3316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_40000, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000002 DASRKH010000000
VERSION     DASRKH010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40000"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="VFC06_00020"
     CDS             <1..503
                     /locus_tag="VFC06_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80322.1"
                     /translation="ALTISAAVALAIGGFSVAPAFADGSGSGGHVLLRAELVGSMPAP
                     GSPVVAGINPGGAPWVNGPSRVRVRDDGRITVQIRGLVIPPPRGTGVNPIASVVATLV
                     CDQLIEASTMPFVLSAAGNGSTREVIAVPRHCDDPVVLIQPAANRAVYIASTVEHERS
                     HAEHDD"
     gene            608..1429
                     /locus_tag="VFC06_00025"
     CDS             608..1429
                     /locus_tag="VFC06_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HZL80323.1"
                     /translation="MEVAAVSIWGVVLAVLLLLFAMAVRIIKQYERGVLLRFGRVHGT
                     LEPGFRLIIPFVDVLHRVSLRIVTLPIQSQGIITRDNVSVGVAAVAYFRVVDPVKSVV
                     AIENVSAAIDQIAQTTLRKVVGQHTLDETLSETDRINLDIREILDVITLAWGVEVTLV
                     ELKDIQLPDTMKRAMAKQAEAEREKRAKIINAEGEHLAAAALGEASDVMMAHPLALQL
                     RNLQSLVEIGVDKNTTVVFPAPIMTTIAELNAFIAREAAAGAAVAPLDRSDGSAA"
     gene            1519..3207
                     /locus_tag="VFC06_00030"
     CDS             1519..3207
                     /locus_tag="VFC06_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HZL80324.1"
                     /translation="MANTDKQPWVKNYQPGVPAEIELPTQSLVAMVEESVLEAGSHPA
                     MEFFGRRTTYTELGDQIDRAAEGLRGLGVRAGDRVALILPNCPQHVVAFYAVLRLGAV
                     VVEHNPLYTSRELRHQFEDHQARVVIAWDKSVSAVRAFPTDIAIDHVVSVNLLKAFPT
                     VKRLALHLPVRSLRESREALTGSAPGTIPWEKLLTHGRIDPEHPRPSVDDLAAIQYTS
                     GTTGNPKGAMLTHYNLYSNALQGEAWMYGAEYRKEIFYAILPMFHAFGMTLYLTYGIR
                     KQALLVLFPKFDPDMILDAMKKSPATVYCAVPPIYERTAMAAKQRGVSLKSCRFCISG
                     AMNLPDHVVELWESMSGGLLVEGYGMTESSPVALGNPFHPTRRTGTIGVPFPSTLMKV
                     VDQDDPTVEVAQGKPGELLLKGPQVFQGYWNNPEETAKSLLPDGWLRTGDVVTVDADG
                     FTTIVDRVKELIITGGFNVAPSEVEAVLRMHPSVIDAAVFGKPLERGGEMVVAAVELE
                     PGAQLDEEGLRAHCRAQLAAYKVPRRIVQIENTPRSMLGKILRKQVREQVLPRL"
BASE COUNT          537 a         1120 c         1133 g          526 t
ORIGIN      
        1 gtgctctcac gatctcagcc gccgttgcac tcgcgatcgg ggggttctcc gtcgccccgg
       61 cgttcgccga cgggtccgga tccggcggtc acgtcctgct gcgtgccgag ctggtcggaa
      121 gcatgcccgc gccagggagt ccggtggtcg cgggcatcaa ccccggcggg gcgccgtggg
      181 tcaacggacc gagccgggtg cgcgtgcgcg atgacggcag gatcacggtg cagatccgcg
      241 gcctggtgat accaccgccg cgcgggaccg gcgtcaaccc catcgcctct gtggtggcca
      301 cgttggtgtg cgaccagctg atcgaagcct ccaccatgcc cttcgtgctg agcgctgccg
      361 gcaacgggtc gacgcgtgag gtgatcgcgg tcccgcgcca ctgcgacgat cctgtcgtgc
      421 tgatccagcc tgccgcgaac cgggccgtct acatcgcctc gacggtcgag cacgagcgtt
      481 cccatgccga gcacgacgac tgaccgactg accgactgac cgactggtcg gcgccgatga
      541 cccgggctcg agcagaggtg ggcaaggccc gatgttgcag gttcgggccg aaggtggact
      601 atggggactg gaggtggctg cggtgtccat ctggggtgtg gtcctcgccg tcttgctgct
      661 gctcttcgcg atggcggtga ggatcatcaa gcagtacgag cggggcgttc tgctccggtt
      721 cggtcgggtg catggaacac tcgaacccgg cttccggctg atcatcccgt tcgtcgacgt
      781 gctccatcgg gtatcacttc ggatcgtcac gctgccgatc cagtcccagg ggatcatcac
      841 ccgggacaac gtcagcgtcg gagtggccgc ggttgcctac ttccgtgtgg tcgacccggt
      901 gaagtcggtc gtcgcgatcg agaacgtgag cgccgccatc gaccagatcg cccagaccac
      961 gctgcgcaag gtcgtcggtc agcacacgct cgacgagacg ttgtccgaga ccgaccggat
     1021 caacctggac atccgcgaga tcctcgacgt catcacgctc gcctggggcg tcgaggtcac
     1081 cctggtcgag ctgaaggaca tccagctgcc cgacaccatg aagcgggcga tggccaaaca
     1141 ggccgaggct gagcgggaga agcgggcgaa gatcatcaac gccgaggggg agcatctggc
     1201 cgcagcagcg ctgggggagg cctcggacgt catgatggct cacccgttgg cgctgcagct
     1261 gcgcaacctg cagagcctgg tggagatcgg cgtggacaag aacaccaccg tggtgtttcc
     1321 ggccccgatc atgaccacca tcgcggagct gaacgcgttc atcgcccgcg aggctgcggc
     1381 gggagccgcc gtggcaccgc tcgatcgcag tgacgggtcc gcagcgtaga aggccagctc
     1441 agctctcagg cgggcctcgg gctgacgtcc ggccgcgtcg tgtcattgcg ggccagaagt
     1501 tcgcggcagg atgtgaccat ggcgaatacc gacaagcagc cctgggtcaa gaactaccag
     1561 ccgggcgtgc cggccgagat cgagcttccg acccagtcgc tcgtcgcgat ggtcgaggag
     1621 tccgtgctcg aggcgggcag ccaccccgcc atggagttct tcgggcgtcg gacgacctac
     1681 accgagctcg gcgaccagat cgatcgggcg gcggagggcc tacgtggcct gggagtgcgg
     1741 gcgggcgacc gcgtggccct catcctgccg aactgcccgc agcacgtcgt cgccttctat
     1801 gcggtgctgc gcctgggcgc tgtcgtcgtg gagcacaacc cgctgtacac gtcgcgcgag
     1861 ctgcgccacc agttcgagga ccaccaggcc cgagtggtga tcgcctggga caagtcggtc
     1921 tcggccgtcc gcgccttccc gaccgacatc gcgatcgacc acgtcgtctc ggtcaacctt
     1981 ctcaaggcgt tccccaccgt caagcgcctc gctctgcacc tgccggtgcg gagcctgcgc
     2041 gagtcccgcg aggcgctgac cggatcggcg ccggggacga tcccgtggga gaagctgctg
     2101 actcacggtc gcatcgaccc ggagcaccca cgtccgtcgg tcgacgacct cgcggccatc
     2161 cagtacacgt cgggcaccac gggcaacccc aagggcgcca tgctcacgca ctacaatctg
     2221 tactccaacg ccctgcaggg cgaggcctgg atgtacgggg cggagtatcg caaggagatc
     2281 ttctacgcga tcctcccgat gttccacgcc ttcggcatga cgctctacct cacgtatggg
     2341 atccgcaagc aggcgctgct ggtgctcttc cccaagttcg accccgacat gatcctcgac
     2401 gccatgaaga agtcgcccgc gacggtctac tgcgctgtcc cgccgatcta cgagcgcacc
     2461 gcgatggccg cgaagcagcg gggcgtctcg ctgaagtcgt gcaggttctg catctccggt
     2521 gcgatgaacc tgcccgacca cgtcgtggag ctgtgggagt cgatgtcggg tgggctcctc
     2581 gtcgagggct acggcatgac cgagtcgtcc ccggtcgccc tgggcaaccc gttccacccg
     2641 acccgtcgca ccggcacgat cggcgtgccg ttcccctcga ccctgatgaa ggtcgtcgac
     2701 caggacgacc ccaccgtcga ggtcgcccag gggaagccgg gcgagctgct gctgaagggg
     2761 ccgcaggtgt tccaggggta ctggaacaac ccggaggaga ccgcgaagtc gctcctgccc
     2821 gacggctggt tgcgaaccgg ggacgtcgtg accgtcgacg ccgacgggtt caccacgatc
     2881 gtggaccgcg tcaaggagct gatcatcacg ggcggcttca acgtcgcgcc ctcggaagtc
     2941 gaggcggtgc tgcgcatgca cccgtccgtc atcgacgccg ccgtcttcgg caagccgctc
     3001 gaacggggtg gcgagatggt cgtcgccgcg gtcgagctcg agccgggcgc ccagctcgac
     3061 gaggaggggt tgcgggccca ctgccgcgcg cagctggcgg cctacaaggt cccgcgccgg
     3121 atcgtgcaga tcgagaacac gccacgctcg atgctcggga agatcctgcg caaacaggtg
     3181 cgcgagcagg tcctgccgcg gctctgacgc ccgggccgcg tcgacgggtg gcgggggagt
     3241 gttcacgacc gtgcccgacc ggccgataag gcagcatgtc gtccggtcca acgcagaact
     3301 acgacgacgc cacgct
//
LOCUS       DASRKH010000003         2462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_8858, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000003 DASRKH010000000
VERSION     DASRKH010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8858"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1232)
                     /gene="serS"
                     /locus_tag="VFC06_00035"
     CDS             complement(<1..1232)
                     /gene="serS"
                     /locus_tag="VFC06_00035"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002858254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HZL80325.1"
                     /translation="MIDLALLRSEPERVRESQRMRGDDVEVVDQVLAADARRRTSLTD
                     FEQARAEQKSLGREVAQAKGDEKAALLARTKDLAQRVKALQAAADAADAEARELLMSI
                     GNLAEPGVPAGGADDFAVLREQGTPRDFAAEGVTVRDHLELGERLKAIDMERGAKVSG
                     ARFYFLTGVGAQLELAILNAAMGVATAHGFSPMITPTLVRPETMRGTGFLGKHSDEIY
                     YLEKDELYLTGTSEVALAGYHTDEILDLNEGPLRYAGWSACYRREAGSAGRDTRGIIR
                     VHQFHKVEMFSYVRSEDAAAEHDRFLAIQESMLQALELPYRVIDTAAGDLGTSAARKY
                     DCEAWLPSQERWMELTSTSNCTTFQARRLAIRERGEDGTRAVATVNGTIGTTRWIVAL
                     LENHQQADGSVYVPPMLRP"
     gene            1270..2427
                     /locus_tag="VFC06_00040"
     CDS             1270..2427
                     /locus_tag="VFC06_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HZL80326.1"
                     /translation="MTDATLAVVIAGSALVIGIAGLILAVTITRRIGTRDPIAIPPFW
                     RKVLRIRALPVALQGEFAPTKRGTERIAFIANPTKAGIDAVREQALRACSNRYLPQPM
                     WLYTTPDDSGHAAAREALDAGAEVVVAVGGDGTVRAIAAELAGTGIPLAIIPMGTGNL
                     FARNLDLPMGDTAKALLIALDGDNAPIDVGWLDLDRGPRAQDERHIFLVIAGAGLDAE
                     MVTGADERLKRRLGWVAYFFAALKHLGSRRMTVSVSVDGSPEVTAQMRTVLFANVGKL
                     PGGLMLVPDASAEDGWLDVATLDARAGIVGWTALFGNVVAQGAGLHMPEILKAYGSSR
                     IDHARGKSIVVAMDHMYKVQADGEGLGLARSVTATVDHGALLVRRRAQQRS"
BASE COUNT          379 a          836 c          835 g          412 t
ORIGIN      
        1 ggacgcaaca tcggggggac gtagacgcta ccgtcggcct gctgatgatt ctccagcagt
       61 gcgacgatcc aacgcgtggt gccgatcgtg ccgttgacgg tcgccaccgc tcgcgtcccg
      121 tcctcgccgc gctcccggat ggcgagtcgc cgcgcctgga acgtcgtgca gttcgacgtg
      181 gaggtgagct ccatccaccg ttcctggctg ggaagccacg cctcgcagtc atacttgcgc
      241 gcggccgaag ttccgaggtc tcccgccgcc gtgtcgatga cgcgatacgg cagttcgagc
      301 gcctgcagca tcgactcctg gatcgccagg aaacggtcgt gctcggcggc cgcgtcctca
      361 gaccggacgt aggagaacat ctcgaccttg tggaattgat gcacccggat gatgccgcgc
      421 gtgtcgcggc cagccgaacc ggcctcgcgc cgataacagg cggaccaccc ggcgtaccgc
      481 agcgggccct cgttcaggtc caggatctcg tcggtgtgat aacccgcgag cgccacctcc
      541 gacgtccccg tcaggtagag ctcgtccttc tcgaggtagt agatctcgtc ggagtgcttg
      601 ccaaggaaac ccgtgccgcg catcgtctcg gggcgcacca gcgtcggcgt gatcatgggc
      661 gagaagccgt gcgcggtggc gacccccatg gcggcgttga ggatcgccag ctcgagttgt
      721 gcgcccacac cggtgaggaa gtagaagcgg gcgccggaga ccttggctcc gcgttccata
      781 tcgatcgcct tgaggcgctc gcccaactcc aggtgatcgc gcacggtgac gccctcggcc
      841 gcgaagtcgc gtggcgtgcc ctgctcgcgc agcaccgcga agtcgtcggc accgcccgcg
      901 ggtacgccag gctccgccag gttgccgatg ctcatgagca gctcgcgcgc ctcggcatcg
      961 gctgcgtcgg cggcggcctg cagggccttg actcgctgcg ccaagtcctt ggtgcgggcc
     1021 aggagcgcgg ccttctcgtc acccttcgcc tgggcgacct cgcggcccag cgacttctgc
     1081 tcggcgcgcg cctgctcgaa gtcggtgagc gaggtgcggc ggcgcgcgtc ggcggccagc
     1141 acctggtcca cgacctcgac atcgtcgcca cgcatccgct gggattcacg cacgcgttct
     1201 ggttctgagc gaagcaaggc tagatcgatc atgatggcga gtgtaacggc gtacacgtac
     1261 gctcaaccca tgacagatgc gacgctcgcg gtggtcattg cgggttccgc actcgtgatt
     1321 ggaatcgcgg gtctgattct cgccgtgacc atcactcggc gcatcggcac gagggatccg
     1381 atcgccatcc cgcctttctg gcgcaaggtg ttgcggatca gggcgttgcc cgtcgcgctt
     1441 caaggggagt ttgcgccgac caagcgcgga acggagcgca tcgcgttcat cgccaatccc
     1501 acgaaggctg gcatcgatgc ggtgcgcgag caggccctgc gcgcctgttc caaccgctac
     1561 ctgccgcagc cgatgtggct ctacacgacg cccgatgatt cggggcacgc ggccgcgcgc
     1621 gaggcgctcg acgctggggc cgaagtggtg gttgccgtcg gtggcgacgg caccgtcaga
     1681 gccatcgccg cagaactcgc ggggaccggg atccccctcg ccatcatccc gatgggaacc
     1741 ggcaatctgt ttgcccgcaa cctcgacctg cccatgggag acaccgcgaa ggcgctgctc
     1801 atcgcgctgg atggcgacaa cgccccgatc gacgtgggtt ggctcgacct ggatcgcggc
     1861 ccgcgcgccc aagacgagcg tcacatcttc ctggtcatcg ccggcgcggg gctcgacgcg
     1921 gaaatggtga caggcgccga cgaacgcctc aagcggcgac tcggctgggt cgcctacttc
     1981 ttcgcagccc tcaagcactt gggttcccgg cgcatgaccg tgtcggtgtc ggtggatggc
     2041 tcccccgagg tgaccgccca gatgcgcacc gtgctgtttg ccaacgtcgg caagttgccg
     2101 ggtggactca tgctggtgcc cgacgcgtcg gcggaggacg gctggctgga cgtggcgacc
     2161 ctcgacgcgc gcgcgggcat cgtcggctgg acagcgctgt tcggcaacgt ggtagcccag
     2221 ggcgcgggct tgcacatgcc cgaaatcctc aaggcgtacg gcagttcgcg catcgaccat
     2281 gcgcgcggca agtcgatcgt ggtcgcgatg gatcacatgt acaaggtcca ggccgacggc
     2341 gagggcttgg gtctcgcgcg cagcgtgacg gccaccgtcg atcacggcgc gctcttggtc
     2401 aggcgtcgcg cgcagcagag gtcttaagag ctagccctcg ccgcgcctga gcgaatccac
     2461 cc
//
LOCUS       DASRKH010000004         3087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_48909, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000004 DASRKH010000000
VERSION     DASRKH010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48909"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="VFC06_00045"
     CDS             <1..723
                     /locus_tag="VFC06_00045"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HZL80327.1"
                     /translation="IIQLGTEAAVLLYFRHDIARIIAAWVRALSGSDGSDRASRFGAK
                     NADARMGWWVILGSVPVVVLGVLFKDSIESSVRNLYITATVLAVFGVILGWVDRVRPA
                     RRELDTMRARDAVLLGLAQAGALIPGVSRSGGTITMARWLGLTRHAAARYSFLLAIPA
                     VVGSAFFELATSYGDLTATGGPGIAATIIATAVAFGVGYFVIVWFLRMITTRSFLPFV
                     VYRLVFAAAIVVLLVTGVIAAT"
     gene            complement(772..1635)
                     /locus_tag="VFC06_00050"
     CDS             complement(772..1635)
                     /locus_tag="VFC06_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020014713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HZL80328.1"
                     /translation="MSEKPPVPPGPPRESIMVAAFEGWNDAGSAASHAIDHVADVWNA
                     QEYGAMDPEDYHDFQVSRPTLSRLPDGERVISWPGTLVSTCAGVSTPDRRISLVRGIE
                     PSMRWRQFCAELLDYAEDLEVSTLITCGALLVDVPHTRPFPTFVSSESEDARLVWDLE
                     RSDYEGPTGILGVLGHDAELRGITVLSMWVGVPHYVAHPPSPKATLALLGQIERLVGA
                     PIDLGDLPDEADAWTRGADELAEDDEEIGEHVRQLESLMDEASLPEASGEAIAAEFEQ
                     FLRRRDGGLGK"
     gene            1660..2343
                     /locus_tag="VFC06_00055"
     CDS             1660..2343
                     /locus_tag="VFC06_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HZL80329.1"
                     /translation="MTTLAFPAAVLWDMDGTLIDTEPYWMEAEADLARRFDVEWTYDD
                     GLSLVGNPLDATAQVLIDRGVRLELGEIISTLVTQVSARAREHMPWLPDARGLLDQVV
                     AAGVPCALVTMSIGALVDAFLDEAGDVFAAVVMGDQVRNGKPDPEAYVMAAQRLGVDP
                     RDCVAIEDSPAGMRSAHASGAVTLAVRRHVPLPFVPGVSRLTSLDGVDPDGIARFFSG
                     HVRDDFAAN"
     gene            complement(2340..>3087)
                     /locus_tag="VFC06_00060"
     CDS             complement(2340..>3087)
                     /locus_tag="VFC06_00060"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HZL80330.1"
                     /translation="PQRATTLGTLVHLVLERLFDVPAAQRDPDAAHALLEPAWDDLVA
                     DRPELAELVADAAERSQFFGDARARVATYFEVENPTRLEPHGREVRLESLLDGGPAIR
                     GVIDRVDKAPDGALRIIDYKSGKTPSSRYGEQADFQMKFYALLVERTEGTRPALMRLL
                     YLRDGGVKELVPTEADIAAVEAKVRSTWTAITMHAAAGEFPTSPSRLCAWCSFQTLCP
                     EFGGTPPPLDPDAVERATGVRPDPTTTAPA"
BASE COUNT          477 a          993 c         1050 g          567 t
ORIGIN      
        1 atcattcagc tcggcactga agcggccgtc ttgctatact tccggcacga catcgcgcgg
       61 atcatcgcgg cgtgggtgag ggcgttgtca ggcagtgacg gcagcgaccg agcctcccgc
      121 ttcggggcaa agaacgccga cgcgcgcatg ggctggtggg tcatcctcgg ctccgtcccg
      181 gtcgtcgtgc tcggagtgct cttcaaggac tcgatcgagt cgagcgtgcg caacctgtac
      241 atcacggcga ccgtgctcgc ggtgtttggc gtgatcctgg gctgggtcga tcgcgtgcgc
      301 cccgcgcggc gtgagctcga caccatgcga gcacgggacg ccgtgctgct tggccttgcc
      361 caagccgggg cgctcattcc cggggtctcg cgttccggtg gcaccatcac gatggcccgg
      421 tggctgggcc tgacgcggca cgctgccgca aggtattcgt tcctgcttgc cattccggcc
      481 gtggtcggtt ctgcgttctt cgaattggcg acgagttacg gcgacctgac cgcaacaggg
      541 ggcccgggga tcgcggcgac catcatcgct actgctgtgg cctttggtgt cggctacttc
      601 gtcatcgtgt ggttcttgcg gatgatcacg acgagatcgt tcctgccgtt cgtggtgtac
      661 cgactcgtgt tcgcagcggc cattgtggtg ttgctcgtga ctggagtcat cgcggccacc
      721 tgacgttccc tggcgggcac gccacgacgc gccgcacggc gccgcgggct actacttgcc
      781 gaggccgccg tcgcgtcggc gcagaaactg ctcgaactca gcggcgatcg cctcgcccga
      841 ggcctccgga aggctcgcct cgtccatgag ggactcgagt tggcgcacgt gttcgccgat
      901 ctcctcgtcg tcctctgcga gttcgtccgc acctcgggtc cacgcgtcgg cctcgtcggg
      961 caggtctccc aggtcgatgg gcgcgcccac gagcctttcg atctggccaa gcaacgccag
     1021 ggtcgctttc ggactcggcg gatgcgccac gtagtgggga acgcccaccc acatcgagag
     1081 caccgtgatg ccgcgcaact ctgcatcatg ccccagcaca cccaggattc cggtgggtcc
     1141 ctcgtaatcc gaacgctcga ggtcccacac gagccgggca tcctcgctct cggaggacac
     1201 gaatgtaggg aacggccgcg tgtgaggaac gtcaacgagg agtgcgccgc acgtgatgag
     1261 agtgctcacc tcgaggtcct ctgcatagtc gagtagttcg gcgcagaatt ggcgccaccg
     1321 catcgatggc tcgattcctc gtaccaacga gatgcgccga tccggcgtcg atacacccgc
     1381 gcacgtggac accagcgttc ccggccagga gatcactctc tccccgtcgg gaagtcgcga
     1441 tagcgtcggc ctgctgacct ggaagtcgtg gtaatcctcg ggatccatcg cgccgtactc
     1501 ctgggcgttc cacacgtcag cgacgtggtc gatcgcgtgg gatgcggcgc ttccggcgtc
     1561 gttccatccc tcgaacgcgg cgaccatgat cgactcgcgc ggcggtcctg ggggaacggg
     1621 gggcttctca ctcatctccc cagcctaaac tgctatctcg tgaccacact tgcatttcca
     1681 gccgccgtcc tatgggacat ggacggaacg ctcatcgaca cggagccgta ctggatggag
     1741 gccgaggcgg atcttgctcg gcggttcgat gtcgaatgga cctacgacga cggactcagc
     1801 ttggtcggca acccactcga cgccacggcc caggtcctca ttgatcgggg agtccgcctg
     1861 gagcttggcg agatcatctc gaccctggtg acgcaggtga gcgcacgagc gcgagaacac
     1921 atgccctggc tgccagacgc gagagggttg cttgaccagg tggtcgccgc gggcgtgccc
     1981 tgcgcgctgg tgaccatgtc catcggggct ctggtggacg catttctgga cgaggccggc
     2041 gacgtgttcg cggcggttgt catgggggat caggtgcgca atgggaagcc cgatccggag
     2101 gcatacgtga tggcggcgca gcggcttggg gtggatccac gggattgcgt cgcgatcgag
     2161 gactctcccg ccggaatgcg cagcgcccat gcttcgggcg cagtgacgct tgcggtgagg
     2221 cgccacgtcc cgctgccgtt cgtgccagga gtgtcccgct tgaccagtct cgacggggtg
     2281 gaccctgacg gcatcgcccg gttcttctcg ggccacgtgc gagacgactt cgccgcgaac
     2341 taggcggggg ccgttgtggt cgggtcgggg cgaaccccgg tcgcgcgttc gacggcatcg
     2401 gggtccaatg ggggaggagt cccgccgaac tcggggcaga gcgtctgaaa ggagcaccac
     2461 gcgcacagtc gtgacggcga cgtgggaaac tcgcccgccg ccgcgtgcat ggtgatcgcg
     2521 gtccaggtgg agcggacttt tgcctccact gcggcaatgt cggcctcggt gggcaccagt
     2581 tccttgacgc cgccgtcgcg gaggtacaga agccgcatca acgcgggtcg cgtgccttcc
     2641 gtgcgttcca ccagcagcgc atagaacttc atctggaagt cggcttgctc gccataacgg
     2701 gaactgggcg tcttgcccga cttgtagtcg ataatccgga gcgcgccgtc gggagcctta
     2761 tcgaccctgt cgatcacccc gcgaatggcg ggccccccgt cgagaagtga ctcgagtctg
     2821 acctcacggc cgtggggttc caggcgcgtc gggttctcca cctcgaagta cgtggccacg
     2881 cgggccctgg cgtcgccgaa gaattgcgag cgctccgccg cgtccgctac gagttcggca
     2941 agctcgggac gatctgcgac gaggtcatcc caggcgggtt cgagcagcgc atgcgcggca
     3001 tcagggtcgc gctgggcggc cggaacgtca aagagtcgtt cgagaaccaa gtgcaccagg
     3061 gtgcccagcg tggtggcacg ttgggga
//
LOCUS       DASRKH010000005         2181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_62199, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000005 DASRKH010000000
VERSION     DASRKH010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62199"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            663..1238
                     /locus_tag="VFC06_00065"
     CDS             663..1238
                     /locus_tag="VFC06_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HZL80331.1"
                     /translation="MSIRHSLLVLLSESDRYGYELRTEFEGRTGGVWPLNVGQVYSTL
                     DRLERDGLVTREDVDGERQVRYSLSDAGRAALSAWWASPVPVSKVGRDDVALKIALAA
                     STAGVDVVDVIRAQRRSVMSALQDLNRAKRSGSDEAWELVADALIFRAEAEVRWLDHT
                     AARLARRSRHPGSQPATEPSKQHTSKSEEVR"
     gene            1238..1975
                     /locus_tag="VFC06_00070"
     CDS             1238..1975
                     /locus_tag="VFC06_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012877481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZL80332.1"
                     /translation="MSTVSPDARVLEMHEVTKTYVDGASTVTALDAASLAVDAGEMVA
                     IMGTSGSGKSTLLAIAGALLAPTSGEVAVCGEVLGQIGARDLARLRRQRIGFVFQDFN
                     LLPTLTALENVALPLDLDGASSATARSEARRALAVVGLRERARSFPDELSGGERQRVA
                     IARATVGRRRLLLADEPTGALDSVTGEAVLHGLRERADDGAAVVVVTHDARHAAWADR
                     IVYLSDGRVVDETRASGPESLLSGGAV"
BASE COUNT          368 a          688 c          744 g          381 t
ORIGIN      
        1 gtagttgcgc gagcggcacc actcacattg aaaccgctcc acaccgccgc ggggtttgat
       61 cggccagcag attgcttgcc ggttgatcgc tcgtgcgcat ttcgcgcagc gaatcgccac
      121 gcgcgcgccg cacgattggt cagccatcag gtcagccata aggcctcgat tcgcccaaaa
      181 tcagcggctc tgcgcccact agcgaatcaa tagagggcct cacgtgccac gacgcgaagt
      241 gcgccacggc ctacgagttg acttccgacg gcaagactcc ctgcgcgagc gtgtcgaatg
      301 gctccgggtt tgcctcccgt gtcggtacca ccatcgaaga aaagttctca atcgctttcg
      361 caacgcgttc ctgagacttc agcccttgat ccgttgaggg caatagggtc tgctgaacta
      421 ctccgtgaca actgtcgtac ggtgccgagg gtcgttgacg tgaggcttgg atgtgccgac
      481 ggccggatgg ttgcaccacg atgcaggagc cccgccgcgg cgcgtgccag cccgagggtg
      541 ggatcttcgt gagaagcgcc ctcggcgagt gctgcggcaa gtttcggaca cgccgcctag
      601 gttgctcacc atagatacgg cgtatatagt tgccacaaca taccgcgtat gcagggggag
      661 tcatgtcgat taggcattca ttgctggtgc tgctcagcga gagcgacagg tacgggtacg
      721 agttgcgtac cgagttcgag gggagaacgg gcggggtgtg gccgctcaac gtcggccagg
      781 tctacagcac tctggatcgc ctcgaacggg acggactcgt gacgagggaa gacgtcgacg
      841 gtgagcgtca ggtgcgctac agcctgtccg atgcggggcg cgcggcgctg tctgcatggt
      901 gggccagccc ggtgcccgta tcaaaggtcg ggcgcgacga cgtggcattg aagatcgcgt
      961 tggcggcgag taccgccggg gtggacgtcg tggatgtgat ccgcgcacag cgccgctcgg
     1021 tgatgtccgc cttgcaggac ctcaaccgtg ccaaacgctc cggaagcgac gaggcgtggg
     1081 aactcgttgc tgatgccctg atcttccgcg cggaggcgga ggtgcgctgg ctcgaccaca
     1141 ccgccgctcg gttggcgcga cggtcccggc atcccgggtc ccagcctgcg actgagccgt
     1201 cgaagcaaca cacaagcaag agtgaggagg ttcgctgatg agcaccgtca gcccggatgc
     1261 acgagtactt gagatgcacg aagtgaccaa gacctacgtc gacggtgcca gcacggtgac
     1321 cgcgctcgac gccgcgtcgc tggcggtgga cgcgggggaa atggttgcga tcatgggtac
     1381 ctccggctcg ggcaagtcga cgctgctggc gatcgccgga gcgctactgg cccccacatc
     1441 tggcgaggtt gccgtgtgcg gcgaggtgct cggccagatc ggtgcacgtg atctggcgcg
     1501 gctgcgaagg cagcggatcg gcttcgtctt ccaagacttc aaccttctgc cgactctcac
     1561 cgcgctcgag aacgtcgcgt tgcccctcga tcttgacgga gcctcgagcg ccaccgcgcg
     1621 ctctgaggcc cgccgtgccc tggccgtcgt cggtcttcgt gaacgcgccc gatcctttcc
     1681 ggacgaactc tcgggaggcg agcgccagcg tgtggctatc gcgcgcgcaa ccgtcgggag
     1741 gcgtcgcctg ctacttgccg acgaaccgac cggagcgctc gactctgtca ccggcgaggc
     1801 cgtccttcac ggccttcgtg agcgcgccga cgacggcgcg gcggtggtcg tggtcaccca
     1861 cgacgcccgg cacgcagcgt gggccgaccg aatcgtgtat ctcagcgatg gacgcgtcgt
     1921 cgacgagacc cgcgcctctg gacccgaatc gctgctgagt ggcggggcag tgtgatcggg
     1981 ttgctcaggt ccatccgcag tgcccggacg gcgctaacgc tcgctgggcg acaggtgcga
     2041 cgcaaccccc gagatgggat tctcgtcgcc gctctcgtgg cactgccatc ggcggcgatc
     2101 atgctgatct ctacggtcgc ggcgagccag caggcgaccg tcgacgagat gctcgccgcc
     2161 gagttgggac aggctgaggc g
//
LOCUS       DASRKH010000006         3608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_22163, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000006 DASRKH010000000
VERSION     DASRKH010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22163"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..2970
                     /locus_tag="VFC06_00075"
     CDS             202..2970
                     /locus_tag="VFC06_00075"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZL80333.1"
                     /translation="MWLRLLRKQGWNDLDAVQERIVAIRAAQGTGEAAFLQEAEARKD
                     VRPAWELMAQYHLAKAAEVLGTYLGQGSVDGHYDIREQLEAQFDRAIGASARGQLVER
                     ETMSRLLARAARVMVDNSIWTVTRAVNSRVTRFVESLTARDRVRPIFEMLPPQRRTLR
                     EEGLLGSGHRSVVVSLPTSSGKTFIAEFRILQALNQFDQENGWVAYLAPTRALVNQLT
                     THLRRDFSHLGTVVEKVSPALEVDGLEASMLTEADGAQQFRILVTTPEKLDLMLRGGW
                     EEKIGRPLTLVVVDEAHGLGFPDRGIKLELLLATINRECRYAQFLLLTPFIRNGAEIA
                     RWLSPDSNKNIDLGVDWTPNDRVVAIARVDRGDGRGEFSLSLRSRHTTRNTLDIPEQI
                     GIGETRPLGLAWSKVKNSPGKIAAATAQVLQKRGTVIVLADTPAATWGIADAFKVEEN
                     KRTLDGDDLAHVQRFLVDEMGQDFPLADLLEYGVGVHHAGMSEDTRALVEWLTENDHL
                     SVLVATTTIAQGVNFPVSGVVFASHQYRLRKSPFRKDMPPQDFWNIAGRAGRVDQGDL
                     GIVALAAQDDAKQQVLEQFIDASVGELNSTLIEMVQAAANAGSLLRLESLSYQPGWSA
                     FVQYLAHTYRQIGDHQQFAAQVEQVLRGTLGFQALRKSHRGWADSLVQGVYAYAERIK
                     GKPLGLVDATGFSWESVNATLARLGQENLPDDVWSPELFAGRRDDLRRMMGLLLQVPE
                     LREPLEDVTGGTGPDGDKLARIICDWVQGRPLTEMAIDYFSQPVEEESDEPRTDPVDA
                     MTQCCRSVFGKLTQTASWGLSALQSLTLGKAIDELSPDEQRSLRNLPARVYYGVNTDE
                     AVALRLLGVPRTAAPALARGLEVTEDEPLHRVRVRLQAADISEWTKAMGERGASYYRV
                     WSIMEGKG"
     gene            2967..>3608
                     /locus_tag="VFC06_00080"
     CDS             2967..>3608
                     /locus_tag="VFC06_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80334.1"
                     /translation="MIADLKPYPAMKDSGVPWAGLVPVHWDLLPNRAMLRKRKVLVGG
                     RHSEYKLLSLTKSGVIVRDVSTSRGKFSADMGTSQEVRIGDLVFCLFDVPETPRTVGL
                     AKRDGMITGAYTVFECSDPLVARFLEAFYIAMDDRKVLSPLYSGLRNTIPPPVFLGAK
                     TPVPPPEEQAAIVRFLDHADRRIRRYIRAKQKLIKLLEEQKQATIHRAVTRGLD"
BASE COUNT          702 a         1076 c         1157 g          673 t
ORIGIN      
        1 gagaccttcc agatcgctag aaccctgaca tggccggagg ggccggtcgc ggctgctgac
       61 tggctcgtgc gtttgggttg tctggccgtc cttggtgatc gctcagcgga ctttcgtcgc
      121 atcgtcacgg aggtcgagct cccagcactt ccgctcgatt ctccggactg gggagtccgg
      181 gtctggtcgt cggtcttgga cgtgtggttg cgtcttctga gaaaacaggg atggaacgac
      241 ctcgatgccg tccaagagcg aatcgtcgcc atccgcgcag cccagggcac aggcgaagcg
      301 gcgtttctgc aggaagccga ggcacgcaag gacgtccgac cggcgtggga gctcatggcc
      361 cagtaccatc tcgcgaaggc cgcggaagtt ctgggaacct atctaggcca gggctctgtc
      421 gatgggcatt acgacatccg cgagcagctt gaggcgcagt tcgaccgagc aattggtgcg
      481 tcggctcgcg gccagcttgt cgagcgagag acaatgtcac gcctccttgc tcgagccgcg
      541 cgcgtgatgg tcgacaacag catctggacg gttacccgtg cggtgaacag cagggtgacc
      601 cgattcgttg agtcgttgac ggctcgcgat cgagtgcgcc cgatcttcga gatgctacca
      661 ccccagagac ggaccttacg ggaagaaggc cttctcggct ctggtcatcg gtccgtcgtg
      721 gtgagcttgc cgacttcgag cggtaagacg ttcatcgcag agtttcggat tctccaagca
      781 ctgaaccagt tcgaccagga gaacggatgg gtcgcgtatc tggcgcccac ccgcgcgctc
      841 gtcaaccagt tgacgacgca cctccgtcgc gacttctcgc acctcgggac ggttgtggag
      901 aaggtgagcc ccgcgctcga agtggatggc ctcgaagcca gcatgctgac ggaggccgac
      961 ggagcacagc agttccggat cctcgtaacc acgccggaga agctggacct catgctgcgt
     1021 ggtggctggg aagagaagat tggccgcccc ctcacgctgg tggtcgtgga cgaggctcac
     1081 ggattgggct tcccggatcg ggggatcaag ctcgagctcc tgctcgcgac catcaaccgc
     1141 gagtgccgct atgcgcagtt ccttctgctg acgcctttca tccgcaacgg agcggagatc
     1201 gcacgctggc tgtcgccaga cagcaacaag aacatcgacc tgggcgtcga ctggacgccg
     1261 aacgaccgcg tcgtggcgat cgctcgtgtc gaccgtggcg atggcagagg cgagtttagc
     1321 ctgtcccttc gatctcgaca cacgactcgc aacaccctgg acatcccgga gcagatcgga
     1381 atcggggaaa ctcggcctct gggcttggcc tggtccaagg tgaagaactc accgggaaaa
     1441 atcgcggcag caaccgccca ggttctccag aagcgcggca cagtcatcgt gctggcggac
     1501 actccggcag cgacatgggg cattgcagac gcgttcaagg tcgaggagaa caagcgaacg
     1561 ctcgacggtg acgacctcgc acatgtacag cggttcctcg tcgacgagat ggggcaggac
     1621 ttccccctgg ctgatctgct cgagtatggc gtcggcgttc accacgcggg catgtcagaa
     1681 gacacgcgtg cgcttgtgga atggctcacg gagaacgatc atttgagtgt actcgtggct
     1741 acgaccacca tcgcccaagg ggtgaacttc cctgtgtctg gcgtcgtgtt tgctagccat
     1801 caatatcgat tgcgaaagtc cccatttcga aaggatatgc ccccccagga tttctggaac
     1861 atcgccggtc gggccggccg cgttgaccag ggagacctcg gcattgtcgc ccttgctgca
     1921 caagatgacg ccaagcaaca ggtgctggag cagtttattg atgcttcagt cggcgagctc
     1981 aactcgacgc tcatcgaaat ggtgcaggct gctgcaaacg ctggctcact gctgcgactg
     2041 gaatcgctgt cgtatcagcc gggttggtca gcgttcgtgc agtatcttgc acatacctac
     2101 cgtcagatcg gggatcacca gcaattcgca gcacaagtcg agcaggtcct acgcggcaca
     2161 cttggcttcc aggcgctgcg gaaaagccac aggggatggg cagacagcct ggtacagggc
     2221 gtgtacgcct atgccgaaag aatcaagggg aagccgcttg gcttggtaga cgctacgggg
     2281 ttctcctggg agtcggtcaa cgccaccctg gcaaggctcg gtcaggagaa cctgccggac
     2341 gacgtatggt cgcccgagtt gttcgcaggc cgcagagatg accttcgccg aatgatgggg
     2401 ttgctgctgc aggtgccgga gctccgagag ccgctcgaag atgtaactgg tgggacgggc
     2461 ccggacggcg acaagctcgc ccgaatcatt tgcgactggg ttcaggggcg ccccctcacc
     2521 gagatggcga tcgactactt cagtcaaccc gtcgaggaag agagtgacga acccagaacc
     2581 gatcccgtcg atgccatgac tcagtgctgc cggagtgtgt tcggaaagct cacgcagacc
     2641 gcgtcctggg gactgtcggc attgcagtcc cttacgctcg gaaaagccat cgatgagttg
     2701 tcgcccgacg aacaacgcag tctgcgcaat ttgcctgcgc gcgtgtatta cggcgtgaac
     2761 acagatgagg ctgtcgctct aaggttgctc ggtgttccca ggactgcggc tccggcactc
     2821 gccaggggcc ttgaggtcac ggaggacgag ccgcttcacc gagttcgcgt ccggctgcag
     2881 gccgccgaca taagcgagtg gacgaaggcg atgggcgagc gtggggccag ctactatcga
     2941 gtatggtcga tcatggaggg caagggatga tcgccgacct caagccctat cccgctatga
     3001 aggactccgg cgtgccgtgg gcgggcttag tccccgtaca ttgggatctc cttccgaacc
     3061 gcgcgatgct gcgcaagcgc aaggtgctag tcggcggtag gcactcggag tacaaacttc
     3121 tctcgcttac gaagtctggc gtcatcgtgc gtgatgtgtc gacaagccgc ggcaagttct
     3181 ctgcggacat ggggacgagc caagaggtgc gcatcggaga cctggtcttt tgcctgttcg
     3241 atgtcccaga gacgccacgg actgtcgggc tcgccaagcg cgatgggatg atcacgggcg
     3301 cgtatacggt attcgaatgt agtgatccgc tcgtggctcg attcctcgaa gcgttctaca
     3361 tcgcgatgga tgaccgaaag gtgctcagtc cactctactc ggggcttagg aacaccattc
     3421 cgccgccggt cttcctcgga gcgaagacgc cggtgccgcc tcccgaggaa caagccgcca
     3481 tcgtccgctt cctcgaccac gcggaccggc ggatccggcg ctacatccgc gccaagcaga
     3541 agctgatcaa gctgctggag gagcagaagc aggccaccat ccaccgcgcc gtcacccgcg
     3601 gcctcgac
//
LOCUS       DASRKH010000007         2329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_8634, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000007 DASRKH010000000
VERSION     DASRKH010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8634"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VFC06_00085"
     CDS             <1..290
                     /locus_tag="VFC06_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80335.1"
                     /translation="LISFVPGGSLVILVFAVLDGQPYDNVYGPDPKSGERYSGPGPGY
                     GQPQQYAQPGYPPQYSQPAAPSFPPHPAGPIAQPPPPPLANPDDPFASPGR"
     gene            294..953
                     /locus_tag="VFC06_00090"
     CDS             294..953
                     /locus_tag="VFC06_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HZL80336.1"
                     /translation="MISWGTVAAFSALAFVVIIIPGPSVLFIVGRALQHGRRDALMSV
                     VGNTGGELVHAALVAAGIGALIAASASAFVVLKIVGGAYLVYLGVQAVRHRRDGLDAV
                     DMGAADARVAPARRPARRVLAESFTVGVTNPKTLVFVAAVLPQFVDPAAGPAWAQILL
                     LGLLFAAIALVSEVAYALAASGARDWFARSPRRLERMRGAGGVMIASLGVVLMASRRV
                     A"
     gene            1121..1303
                     /locus_tag="VFC06_00095"
     CDS             1121..1303
                     /locus_tag="VFC06_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80337.1"
                     /translation="MEDTEGVEARARARAAMERLGQRLAEGEPPDASADRDLAELAAR
                     IDATLWNHHCTETPAR"
     gene            1300..1629
                     /locus_tag="VFC06_00100"
     CDS             1300..1629
                     /locus_tag="VFC06_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80338.1"
                     /translation="MSDLAVEGDALHHVARTVRTVTAEFLSAGAQAHDAAQHVGHPGL
                     AQRVTAFADQWDIHRERTTRLLERLADALDAVNDTFADLDREAAATLPYSSGGLGTAV
                     GQSATKA"
     gene            1629..2186
                     /locus_tag="VFC06_00105"
     CDS             1629..2186
                     /locus_tag="VFC06_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80339.1"
                     /translation="MPAPIPWGGALATPAGDPLALDQAVDRYAHTEAAIARAADDLLA
                     AVFDGEGLAIAALSQSVGKAEAALVAARGRYQGTTSALREYAVDLATFHRAANAAIDR
                     EHQALRRLDWAEQRKAEAGDRWRLATMNPAHVADLDSAYGDFRAAVSARDAAQDAVDA
                     PRSDYGRATAALDDAAQVAIARIVA"
BASE COUNT          315 a          808 c          843 g          363 t
ORIGIN      
        1 ggcttatcag cttcgtgccc ggcggcagcc tcgtgatctt ggtgttcgcc gtgctcgacg
       61 ggcagcctta cgacaacgtg tacggacccg accccaagtc gggcgagcgc tactccggcc
      121 ccggccccgg ctacggccag ccccagcagt acgcgcaacc cggttatcct ccgcagtact
      181 cgcagcctgc ggcgcccagc ttcccgccgc acccggccgg accgatcgcg cagccgccgc
      241 caccgccgct cgcgaatcct gacgatccgt tcgcctcgcc aggccggtag cgcgtgatct
      301 cctggggaac ggtggcggca ttcagcgcgc tcgcgttcgt ggtcatcatc attcccgggc
      361 cgagcgtgct gttcattgtg ggccgcgcgc ttcagcacgg ccggcgcgat gcgttgatgt
      421 ccgtggtggg caacaccggc ggagaactgg tgcatgccgc gctggtagcg gcaggaatcg
      481 gggcgctcat cgccgcatcg gcctccgcct ttgtggtgct caaaatcgtc gggggcgcgt
      541 acttggtgta cctcggggtg caggcggtgc ggcatcggcg ggatggtctc gacgcggttg
      601 acatgggcgc ggccgatgca cgggtggcgc cagcccgccg ccccgctcgg cgggtgctgg
      661 ccgagtcgtt cacggtgggc gtcacgaatc ccaagacgtt ggtgtttgtg gcggccgtgt
      721 tgccgcagtt tgtcgacccg gccgcagggc cagcgtgggc gcagatcctc ctcctcggcc
      781 tgttgttcgc tgcgatcgcc ctggtgagcg aggtcgccta cgcgttggcg gcatcgggcg
      841 cgagggactg gttcgcgcgt tcgccgcgcc gtcttgagcg gatgcgcggc gcgggcgggg
      901 tgatgatcgc ctcgctgggc gtggtgctga tggcgtcgcg tcgggtggcg tgacacggtg
      961 cgatctgtac gacgtgcgcg gctggcggta gcggccggtt tcccggcgtc gtccaccgcc
     1021 tgattgcctg caacctttcc accagccgag cggcggtgcg cctcatcggg cgcccgcccc
     1081 gccctacctt cgagtcgtcg cgaggcgcag aggagggacc atggaggaca ccgaaggggt
     1141 tgaggcgcgc gcgcgagcgc gtgcggcgat ggaacgtctg ggccagcgcc ttgctgaggg
     1201 tgagccgccg gacgcgtcgg ccgaccgcga cctcgcagaa ctcgctgctc gcatcgacgc
     1261 gacgctgtgg aaccaccact gcaccgaaac tcccgcgcga tgagcgacct tgccgtcgaa
     1321 ggcgatgccc tccaccacgt cgcacgcacg gtgcgtaccg tcaccgccga attcctctcg
     1381 gcgggcgctc aggcgcacga cgccgcccag cacgtcggcc atcccggcct ggcgcagcgg
     1441 gtgaccgcgt tcgcggacca gtgggacatc catagggaac gcacgacgcg cctgctggag
     1501 cggcttgccg atgcgctcga cgcggtgaac gacacgttcg cggacctcga cagagaggcc
     1561 gccgcgacgt tgccctactc aagcggtggc ctcggcaccg cagtgggtca gtcggcgacg
     1621 aaggcatgat gccggccccg atcccgtggg gcggcgcgct ggccaccccg gccggcgacc
     1681 ccctcgctct cgaccaggcg gtggaccgct acgcgcatac cgaggcggcc atcgcgcgcg
     1741 ccgcggacga cctgctggcg gcggtcttcg acggtgaggg gctcgccata gcggcactca
     1801 gtcaatccgt gggcaaggcc gaggcggccc tggtcgccgc tcgcgggcga taccagggca
     1861 ccacctccgc cttgcgcgag tatgcggtgg acctggcgac cttccatcgt gccgcgaacg
     1921 cggccattga ccgcgagcac caagcgctca ggcggctcga ctgggccgag cagcggaagg
     1981 ccgaggcggg cgaccgttgg cgccttgcga cgatgaaccc ggcgcatgtc gcagacctcg
     2041 acagcgcgta cggcgatttc cgggcggccg tctcggcgcg agacgcagcc caagacgccg
     2101 ttgacgcgcc gcgctccgat tacgggcgcg cgacagccgc acttgacgac gccgcgcagg
     2161 tggccatcgc gcgcatcgtc gcctagttcg atgggacgaa tgacggcttc acggaccacg
     2221 tcgggcacgc gttcgccgcg gcggggcaga tgctgacgga cctgactcgg tggtcaaggg
     2281 acttccttgc agcggtcctc gacgtggtgg tcgctgcggt ggggctgta
//
LOCUS       DASRKH010000008         2156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_3949, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000008 DASRKH010000000
VERSION     DASRKH010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3949"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..589
                     /locus_tag="VFC06_00110"
     CDS             161..589
                     /locus_tag="VFC06_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80340.1"
                     /translation="MLGKGDGSEFAAEFAADVFRTKLRETFTALSTGVSQAGTAEQTA
                     TVRDAGVQSLGTNDGGAIVVGAIDQTLTIKRTVADSTLEVGGGLTYGGDAQVKGTLTA
                     TYVITVAFHVPPAGSDQPTQVLGAEQVLTGVTRDDSTSPD"
     gene            607..1494
                     /locus_tag="VFC06_00115"
     CDS             607..1494
                     /locus_tag="VFC06_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZL80341.1"
                     /translation="MNQPLNVHGAVDLSSLAAPRRPAGATPVPGGVVIDVTEAGFEAL
                     VQRSTTVPVVVDLWAEWCQPCKTLSPILERLAAEYGGRFQLAKVDVDANPQIAAAFQV
                     QSIPTVVALLGGQPVPLFQGAYPEAQVRQILDQLLAVAAQNGITGTVPPEAATGPEPE
                     PELPPLHAAGLAAIETGDYVTAEAAYRQALKENPGDAEAKAALAQVLLLQRLEGLDLA
                     VAVADARDGDLDSQLLAADAELATGLLSAAFSRLLALVRAGGELREPARVRLVEYFDI
                     VGPVPEAAQARRALASALY"
     gene            complement(1562..>2156)
                     /locus_tag="VFC06_00120"
     CDS             complement(1562..>2156)
                     /locus_tag="VFC06_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HZL80342.1"
                     /translation="MRKMPGDWWQQLAGVRALLAYQWSHPGKQLLFMGQEFAQEAEWN
                     ESRGLDWWHLDNPMHSGVADLVARMNTIYKASPALWADDFSHEGFEWIEAGDGDHNTI
                     VFLRKTPDFSERMVCIVNFAGIPHEGYRVGLPHGGAWLEVLNTDAIEYGGSGVGNLGR
                     VEAEEIPWQGRPFSVRLRVPPLGALWLVPDESSIPEE"
BASE COUNT          318 a          783 c          747 g          308 t
ORIGIN      
        1 gctcggccgt ccccgcctgg gagacgccgg tggacagcgc ggtgaacgtc tcgcgaagtt
       61 tcgtgcggaa gacgttcgcc gcggagttcg cggcgaactc ggacccgtcg cccttgccga
      121 gcacgtcggc ataacgctca ctctggagcg ttatgccgac gtgctcggca agggcgacgg
      181 gtccgagttc gccgcggagt tcgcggcgga cgtcttccgc acgaaacttc gcgagacgtt
      241 caccgcgctg tccaccggcg tctcccaggc ggggacggcc gagcagacgg ccaccgtgcg
      301 ggatgccggg gtccagtcgc tggggacgaa tgacggcggc gcgatcgtcg tcggcgccat
      361 cgaccagacc ctcacgatca agcggaccgt ggcggactcc acactcgagg tcggtggggg
      421 actcacctat ggcggcgacg cccaggtcaa gggcaccctc acggcgacgt acgtgatcac
      481 cgtggccttc cacgtgcccc cggcgggcag cgaccagccc acccaggtcc tgggggccga
      541 gcaggtgctc acgggcgtca cgcgcgacga ttcgacctca cccgactgag acatacgatt
      601 gggtgcatga accaaccact caatgtgcac ggcgccgtcg acctctcgtc cctcgcggcc
      661 ccgcgtcggc ccgcgggcgc gacgcccgtt cccggcggcg tcgtcatcga tgtgacggag
      721 gccgggttcg aggccctggt ccagcgctcg accaccgtcc ctgttgtcgt cgacctctgg
      781 gccgaatggt gccagccctg caagaccctg agccccatcc tcgagaggct cgccgcggag
      841 tacggcggcc ggttccagct cgccaaggtg gacgtggacg ccaacccgca gatcgcggcc
      901 gccttccagg tgcagtcgat ccccacggtg gtcgcgctgc tcggtggcca gccggtcccg
      961 ctcttccagg gggcctaccc cgaggcgcag gtgcgccaga tactggacca gctgctcgcc
     1021 gtcgccgcac agaacggcat cacgggcacc gtgccgcctg aggcagcgac cgggcccgag
     1081 cctgagcccg aactcccgcc gctgcacgcg gccggactcg cggccatcga gacgggcgac
     1141 tacgtgacgg ccgaggccgc ataccgccag gcgctcaagg agaacccggg cgacgccgag
     1201 gcgaaggccg cgctcgcgca ggtgctgctc ctgcagcgcc tcgaaggact ggacctcgcc
     1261 gtcgccgtcg ctgacgcgcg ggatggcgac ctggactcgc aactgctcgc cgccgacgcc
     1321 gagcttgcca ccgggctcct gtcggcggcg ttctcccgcc tcctcgccct cgtccgcgcc
     1381 ggcggggagc tgcgtgagcc ggcacgggtg cgcctcgtgg agtacttcga catcgtcgga
     1441 cctgtgcccg aggcggcgca ggcacgccgg gcgctggcga gcgcgctgta ctgagtccgg
     1501 tcggccgccc gaccgggcat gcgaaagggg gccccgaggg gcccccttca gcgcgtgtgc
     1561 actactcctc cgggatggaa ctctcgtccg gcacgagcca gagcgcgccg agtggcggca
     1621 cgcgcaggcg caccgagaac ggccggccct gccacgggat ctcctcggcc tcgacccgcc
     1681 cgaggttgcc cacacccgag ccgccgtact cgatggcgtc cgtgttgagc acctccagcc
     1741 aggcgccgcc gtgggggagc ccgacacggt agccctcgtg cgggatgccg gcgaagttca
     1801 cgatgcacac catgcgctcc gagaagtccg gcgtcttgcg caggaagacg atcgtgttgt
     1861 ggtcgccgtc gccggcctcg atccattcga agccctcgtg ggagaaatcg tcggcccaga
     1921 gtgccggcga ggccttgtag atcgtgttca tccgggccac caggtcagcc acgcccgagt
     1981 gcatggggtt gtcgaggtgc caccagtcga ggccgcggga ctcgttccac tcggcctcct
     2041 gggcgaactc ctgacccatg aacaggagtt gcttgcccgg gtgcgaccac tggtaggcga
     2101 gcagcgcgcg gacgcccgcg agttgctgcc accagtcacc gggcatcttg cgcatg
//
LOCUS       DASRKH010000009         2648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_48778, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000009 DASRKH010000000
VERSION     DASRKH010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48778"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VFC06_00125"
     CDS             complement(<1..710)
                     /locus_tag="VFC06_00125"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZL80343.1"
                     /translation="MSTLAGVHFAVDAAQPAHASAVLIELGIVLLVLAGLGRLAARIG
                     LPSVPLYLLAGLLMGEGGLFKVDAGEEFLAIGAQIGVVLLLLLLGLEYTPADLKQGLK
                     TTWLAGILDGLANTLPGIAFGLILGWSPAASLLLGGVTYMSSSGIIARLLDDFDRYAN
                     RETPVILSLLVMEDIVMAVFLPVIAVILVGSSIAQGAIAVGVALGLVAIAFVVSFRFS
                     SHVSRAVHSRSRELLLLSV"
     gene            complement(714..1193)
                     /locus_tag="VFC06_00130"
     CDS             complement(714..1193)
                     /locus_tag="VFC06_00130"
                     /inference="COORDINATES: protein motif:HMM:NF014171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter regulatory subunit"
                     /protein_id="HZL80344.1"
                     /translation="MEIFETPLPGIGVRYEFTSELGDHVGVVIRRDGKRDVALYDRQD
                     PDSCRGTMELTDSDASKLAELLGGTSITARLDSLRHMVQGLAIEWVTMPASGGLSGMT
                     IGEGRIRTTTSASVVAVIRGDADIPGPGPEFTLEAGDTVLVMGSENAVGQARVILTG"
     gene            complement(1262..1687)
                     /locus_tag="VFC06_00135"
     CDS             complement(1262..1687)
                     /locus_tag="VFC06_00135"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZL80345.1"
                     /translation="MSTQRLSLITLPVDDLGAAIDFYQRAMGWVIKMRFGDDIAFVQM
                     NGFVAALWSRASWEAERLDFEPPGYTSFAINFTRPPEVDEVAARWAEAGGEVAKAPGT
                     VYWGGYSGYVRDPWGNLIELAVNDALFITEEGYTEVRGS"
     gene            complement(1720..>2648)
                     /locus_tag="VFC06_00140"
     CDS             complement(1720..>2648)
                     /locus_tag="VFC06_00140"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZL80346.1"
                     /translation="VVASLLPGVLIAGGAAALVAAAVAAVLVTRTIMGPLGSIQAASR
                     RVANGDYTQSVPTPRDEELAAVAHDINALSQRLAETEARRTRLIDEVAHEMRTPITTI
                     KGSMEALLDEVVEPGPEVYARVADEASRLQRLAEDLSTLSRAEEHTLRMELSPIDAAD
                     VASEVCERMRPQFEHAGVALLMDGSSAPVEGDAHRLAQVVINLVGNALGHTHAGDTVT
                     VTSGSDAATSWVSVTDTGQGIAPDELGRIFERFYRVPDASTPGGRGIGLTIARSLVEA
                     HGGTLGVTSEGVGRGANFVVTLPRHEARDALG"
BASE COUNT          462 a          873 c          892 g          421 t
ORIGIN      
        1 acggacagca gcagcagttc gcgcgaccgc gagtgcactg cgcgcgacac gtgagaggag
       61 aagcggaagg agacgacgaa cgcgatggct accaggccga gggcgacccc cacggcgatg
      121 gccccttgag caatgctcga accgaccagg atcacggcaa tgaccggcag gaacaccgcc
      181 atcacgatgt cctccatgac cagcaggctg aggatgacgg gggtctcgcg gttggcatac
      241 ctgtcgaagt cgtcgagcaa gcgggcaatg attcccgatg acgacatgta cgtcacgccg
      301 ccgagcagca gcgatgccgc gggagaccag ccgaggatga gcccgaaggc gatgccgggg
      361 agggtgttcg ccaagccgtc gagaatcccc gccagccagg tggtcttgag tccttgcttg
      421 aggtcggcgg gtgtgtattc aagtcccagc agtagcagga gaaggaccac gccgatctga
      481 gcgccgatcg ccaggaactc ttcgccggca tcgaccttga agaggcctcc ctcgcccatc
      541 agcaggccag ccagcaggta cagcgggacc gagggaagac cgattcgcgc cgcgagtcgt
      601 cccaggccgg cgagcaccag cagcacaatg cccaactcga ttaacaccgc cgacgcgtgg
      661 gccggttggg cggcgtccac cgcaaagtgg acgcctgcga gggtggacac ggctcagcca
      721 gtcagaatga cgcgggcctg gcccacggca ttttcgctac ccatgacgag aactgtgtcg
      781 ccggcctcga gtgtgaactc gggcccgggg cctgggatgt cggcgtcgcc acggatcacg
      841 gctacgacgg aggcagaggt tgtggtgcga atgcgacctt cgccgatggt cattccgctg
      901 aggccgccgc ttgcgggcat cgtcacccat tcgatcgcca ggccctgcac catgtggcgg
      961 agcgagtcga gcctggcggt gatgctggtg ccacccagca attcggccaa tttgctggcg
     1021 tcgctgtccg tgagttccat ggtgccgcga cacgagtcag ggtcttgcct gtcgtagagc
     1081 gcgacgtcgc gcttgccgtc gcggcgtatc acgacgccca cgtggtcgcc aagctccgag
     1141 gtgaactcgt agcgcacgcc gatgcctggc agtggggtct cgaagatctc catggcccta
     1201 ctctgccatg gagggagtcc cgcggtcact cgggaccggc cgggatatcg ccaccggcgc
     1261 gctagctgcc gcgtacttcc gtgtagccct cctcggtgat gaagagagcg tcgttcaccg
     1321 ccagttcgat gaggttgccc cacgggtcgc gcacgtaccc cgagtacccg ccccaataca
     1381 cagtgccggg cgccttcgcg acctcgccac cggcctcggc ccaccgcgcg gccacctcgt
     1441 ccacctccgg cggacgagtg aagttgatgg cgaacgaggt atatccaggg ggctcgaagt
     1501 cgaggcgttc cgcctcccag gaggcgcgcg accacagggc cgcgacgaag ccgttcatct
     1561 gcacaaaggc gatgtcgtcg ccaaagcgca tcttgatgac ccagcccatg gccctctgat
     1621 agaagtcgat ggcggcaccg aggtcgtcga cggggagggt tatcagagaa agcctttgcg
     1681 tgctcatggc agaagtatgg tccgttcagg ggaaacccgc tagccgagag cgtcacgagc
     1741 ttcgtgtcgc ggaagtgtca cgacgaaatt ggcgcccctt ccgacgccct ccgacgtgac
     1801 gcccaaggtg ccgccgtgcg cctcgacgag cgatcgcgca atggtgagtc cgatgccgcg
     1861 tccgcccggc gtcgacgcat cgggaacgcg gtagaagcgt tcgaagatgc gacccagctc
     1921 gtcaggggcg attccttggc ccgtgtccgt gacggacacc cacgacgtgg ccgcgtcgct
     1981 tcccgacgtg accgtgacgg tgtcgcccgc gtgcgtgtgt cccagtgcgt tgcccacgag
     2041 gttgatcacc acctgtgcga ggcggtgcgc atcgccctcg acgggagcac tcgagccgtc
     2101 catgagcagg gcgacgccgg cgtgttcgaa ttgcggccgc atgcgctcgc acacctccga
     2161 ggcgacatcg gcggcgtcga tcggcgacag ctccatgcgg agcgtgtgct cctcggcacg
     2221 cgacagcgtc gacaggtcct ccgccaggcg ctgcaatcgc gaggcctcat cggcgactcg
     2281 cgcgtagacc tcgggtcccg gttcgaccac ctcgtcgaga agcgcctcca tcgagccctt
     2341 gatcgtggtg atgggggtgc gcatctcgtg tgcgacctcg tcgatgagtc gggttcgacg
     2401 agcttccgtc tccgcgagcc tttgcgacag cgcgttgatg tcgtgggcca ccgccgcgag
     2461 ttcctcatcg cgtggggtgg gcaccgactg cgtgtagtcg ccgttcgcga cgcgccgtga
     2521 cgccgcctga atcgagccga gtgggcccat gatggtccgc gtcacgagca ctgcggccac
     2581 cgcggcggcg acgagtgctg cggcgccccc cgcgatcagc acccctggaa gcaggctggc
     2641 cacgacgt
//
LOCUS       DASRKH010000010         3788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_35531, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000010 DASRKH010000000
VERSION     DASRKH010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35531"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..1693
                     /locus_tag="VFC06_00145"
     CDS             206..1693
                     /locus_tag="VFC06_00145"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HZL80347.1"
                     /translation="MGVLRHHVAGREFGSTERTGTISNPATGAVVGEVAYASTAEADA
                     VIAAAKAALPAWKVTGLIKRADVFFRMRNLLVERQDELAAIVTREHGKVLADAKGEIS
                     RGLENIEFAAGLVHLLKGEFSEQVSRGVDVHSVKQPVGVVACITPFNFPVMVPLWMTA
                     SAIACGNTVVLKPSEKDPGASDFLVKLFEEAGLPEGVLNVLHGDKVAVDALLESADVS
                     AISFVGSTPVARLIYARGTAAGKRVQALGGAKNHMVVMPDADLDGAADAAVSAAYGSA
                     GERCMAISVLVAVGDATADALVAKVSERMGKLVIGDGTDPASEMGPLITREHRDRVAS
                     YVTGAAADGATVVVDGTAQHFDGDGFFIGVSLVDHVRPGMKMYDDEIFGPVLSVVRVE
                     TYGEAVALINANSFANGTAIFTRDGGTARRFEVDAEVGMVGINVPIPVPVGAYSFGGW
                     RNSLFGDSHIYGPESVHFYTRSKVVTSRWPEPSESQVDLGFPSNH"
     gene            1702..3066
                     /locus_tag="VFC06_00150"
     CDS             1702..3066
                     /locus_tag="VFC06_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HZL80348.1"
                     /translation="MSTQGGTPMAPDEASVRTNDRQHVFHSWSVQGAINPMPVAGGLG
                     SEFWDYDGNKYLDFGSQLVNLNLGHQHPDLIAAIQRQAGRLATIQPALANDVRGELAR
                     LVTEAAGESFAKVFFTNGGADANENAVRMARLFTGKRKVMAMYRSYHGSTSTAITLTG
                     DPRRWANEPSDASVVHFHGPYLYRSPFYSVSAEQETERALEHLEQTIVLEGASTIGAI
                     ILETIVGTNGVLMPPPGYLKGVRALCDKYGILYIADEVMVGFGRVGEWFAYQAHDVVP
                     DLVTFAKGINSGYVPLGGVIISATISEFFDDRMFPGGLTYSGHPLACAVGLATFEVLE
                     RDGILAHVKDLGDRVIEPILTSWLEKHPCVGDVRGTGLFWAIELVKDKATREPLVPFN
                     ASGADAAPMAAFVAACKASGLWPFAHFNRTHVCPPLVITEEELRRGLAIIDAALTAVD
                     AAIA"
     gene            3118..>3788
                     /locus_tag="VFC06_00155"
     CDS             3118..>3788
                     /locus_tag="VFC06_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZL80349.1"
                     /translation="MTTIDSTPVVVGGPSLPQERRLVTAIPGPRSQELMDRKAAAVAA
                     GVGHTAPISAVAAGGGVVVDADGNSLIDLGSGIAVTGVGNSHPAVVAAIQDQAARFTH
                     TCFMVSPYEGYVAVAEALNRVTPGDFPKKSALFNSGAEAVENAVKIARKFTGKPAVVA
                     FDHAYHGRTNLTMALTAKSMPYKSGFGPFASEIYRAPMSYPYRDGLSGAAAAAKAISV
                     IEKQVG"
BASE COUNT          631 a         1247 c         1246 g          664 t
ORIGIN      
        1 ggctcgtcga gcgcctctgc cacagtcggc aaggcgtcga gtcccaggtg gtccgggccc
       61 gccgcgtccg tacggaatgt ccggacgcta ctcacatcac gacggtcgga ggagggcatg
      121 gcccgattct atgtgcgatt ctgggcagat acgcatctcg taggtatcgg gcaatgtgtc
      181 cggtctgttt cgtggaggga aagccatggg cgtacttcgc catcacgtcg ccggccggga
      241 gttcggttcg accgaacgca ccggcaccat ctccaacccc gcgacgggcg cggtggtggg
      301 cgaagtggcc tacgcctcga cggccgaggc cgatgccgtg atcgccgcgg ccaaggcggc
      361 cttgcccgcg tggaaggtaa ccggactcat caagcgcgcc gacgtgttct tccgcatgcg
      421 caacctcttg gtcgaacgcc aggatgagct cgccgcgatc gtcacgcgcg aacacggcaa
      481 ggtcctcgcg gacgcgaagg gcgagatcag tcggggtctt gagaacatcg agttcgcagc
      541 tggcctcgtg cacttgctca agggcgagtt ctccgagcag gtctcgcgcg gcgtcgacgt
      601 ccactcggtg aagcagcctg tgggcgtggt cgcgtgcatc accccgttca actttccggt
      661 gatggtgccg ctgtggatga ccgcctcggc gatcgcgtgc ggcaacaccg tggtgctcaa
      721 gccgagcgag aaggaccctg gcgcgtccga cttcctcgtc aagctcttcg aagaggcggg
      781 cctgccggag ggggtcctca atgtgctcca cggggacaag gtcgctgttg acgcacttct
      841 ggagtcggcc gacgtttccg ccatcagctt cgtcggctcc acccccgtgg cgcggttaat
      901 ctacgcgcgc ggcacggccg ccggtaagcg cgttcaggcg ctcggcggtg ccaagaatca
      961 catggtcgtc atgcctgacg ccgaccttga cggggcggcc gatgccgcag tctctgcggc
     1021 ctatggttcg gcgggggagc gctgcatggc gatttccgtc ctggttgcgg tgggcgacgc
     1081 gacggccgac gccctcgtgg cgaaggtcag cgagcgcatg ggcaagctcg tcatcggcga
     1141 tggcaccgac ccggcctccg aaatgggtcc gctcatcacg cgagagcacc gtgacagggt
     1201 cgcgtcgtac gtgacgggtg cggccgcaga tggcgcgacc gtcgtggtcg acggcaccgc
     1261 tcagcacttc gatggcgacg gcttcttcat cggcgtcagc ctggtggacc acgtccgccc
     1321 aggcatgaag atgtacgacg acgagatctt cggccccgtc ctgtccgtgg tccgtgtcga
     1381 gacgtacggg gaggcggtgg cgcttatcaa cgcgaactcg ttcgccaacg gcaccgcgat
     1441 cttcactcgc gacggtggta cggcacggcg cttcgaggtc gacgccgagg taggcatggt
     1501 gggaatcaac gtgccgatac cggtgcctgt gggcgcgtat tcgttcggtg ggtggcgcaa
     1561 ttcgctcttt ggcgactcgc acatctacgg accggaatcg gtgcacttct acacgcgcag
     1621 caaggtcgtg acctcgcgct ggcccgaacc cagcgagtcg caagtggacc tcggattccc
     1681 tagcaaccac tgagcattga gatgagcaca cagggaggta cccccatggc cccagatgag
     1741 gcctcggtca ggacaaacga ccgtcagcat gtcttccact cctggagcgt ccagggcgcc
     1801 attaacccga tgccggtcgc gggtgggctc ggatcggagt tttgggacta cgacggaaac
     1861 aagtacctcg actttggctc ccagctggtg aatctcaact tgggacacca gcaccccgac
     1921 ctcatcgctg caatccaacg gcaggccggg cgtctcgcga caattcagcc ggcgctcgcc
     1981 aacgacgtgc gcggcgagct cgcacgcctg gtgaccgagg cggcggggga gtccttcgcc
     2041 aaggtcttct tcaccaacgg cggcgccgac gccaacgaaa acgcggtccg catggcgcgc
     2101 ctgttcacgg gcaagcgcaa ggtcatggcc atgtaccgca gctatcacgg cagcacgtcg
     2161 acggcgatca cgctgaccgg agacccgcgc cgttgggcga acgaaccctc ggatgcctcg
     2221 gtggtgcatt tccacggccc ctatctgtat cgctcgccgt tctattccgt cagcgccgag
     2281 caggagacgg aacgcgcgct cgaacacctc gagcagacca tcgtgctcga gggcgcgtcg
     2341 accatcggcg ccatcatcct cgagacgatc gtgggcacca acggcgtgct gatgcccccg
     2401 cccggctacc tcaagggtgt ccgcgcgctg tgcgacaagt acggcatcct ctacatcgcc
     2461 gacgaggtga tggtcggctt tggccgcgtc ggcgagtggt tcgcgtacca ggcgcatgac
     2521 gtggttcccg acctggtgac gtttgccaag ggcatcaact cgggttacgt cccgctcggt
     2581 ggcgtcatca tctcggcgac catctcggag ttcttcgacg accgcatgtt cccgggtggt
     2641 ctgacgtatt cgggtcaccc gctcgcgtgc gccgtgggac tggcgacctt cgaggtgttg
     2701 gagcgcgacg ggatcctcgc tcacgtcaag gacctcggcg atcgcgtcat cgagcccatt
     2761 ctcacgagct ggcttgagaa gcacccgtgc gtgggtgacg tccgaggcac cggcctcttt
     2821 tgggcgatcg aactggtcaa ggacaaggcc acgcgggagc ctttggtgcc gttcaacgca
     2881 tcgggcgccg atgccgcacc gatggcggcc ttcgtggccg catgcaaggc atcgggactg
     2941 tggccgttcg ctcacttcaa ccgcacccac gtgtgcccgc cgctcgtcat caccgaggaa
     3001 gagctgcgca gaggactcgc gatcatcgac gccgccttga ccgccgtaga cgcggcgatt
     3061 gcttgagcct cactcacccc ttttcactgc acccccaacc ggaataggct cacgcgcatg
     3121 accacaatcg attcgacgcc cgtcgtggtg ggtgggccct cgcttcccca agagcgcagg
     3181 ctagtcaccg cgatccccgg tccgcgctcc caggaactga tggaccgcaa ggccgccgcg
     3241 gtcgccgcgg gcgtggggca caccgcgccg attagtgccg tggcggcggg tggcggagtc
     3301 gtcgtcgacg cggatggtaa ctcgctcatc gacctcggct ccggaattgc cgtgacgggc
     3361 gtgggcaact cccaccccgc cgtcgtggcc gcgattcagg atcaggccgc gcgcttcact
     3421 cacacgtgct tcatggtctc tccttacgag ggctacgtgg ccgtggccga ggccctcaac
     3481 cgggtgacgc ctggcgactt ccccaagaag tccgcgctgt tcaactctgg tgccgaggcg
     3541 gtcgagaacg cggtcaagat tgcgcgcaag ttcacgggca agcccgccgt cgtcgccttc
     3601 gaccacgcgt atcacggacg caccaacctc acgatggcgc tgaccgccaa gtcgatgccg
     3661 tacaagagcg gctttgggcc tttcgcgtcc gagatctaca gggcgccgat gtcgtaccca
     3721 taccgcgacg ggctgagtgg agccgccgcc gccgcgaagg cgatctccgt gatcgagaag
     3781 caagtggg
//
LOCUS       DASRKH010000011         3961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_22095, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000011 DASRKH010000000
VERSION     DASRKH010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22095"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VFC06_00160"
     CDS             complement(<1..248)
                     /locus_tag="VFC06_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80350.1"
                     /translation="MTDALSNSGDVRPIPDPTARTIETLEREITSVRDWAELQLKSAE
                     IINAERFASEHRADAIAEAHRLELKADNQRTVETAMIAA"
     gene            480..1232
                     /locus_tag="VFC06_00165"
     CDS             480..1232
                     /locus_tag="VFC06_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80351.1"
                     /translation="MRSPVRVIVWAVALIALLGGATGSTADARIEPLPVGHYSTVEMA
                     SVDEVCAGQPRIGLAVDIAAWQEVRSLRDLGLPACDVTWRFGSLVDWSGKAWADATTG
                     QVTILINGDPANYAGDSDPAWAIRSNIRHEFGHALVFMAGWTSGQDHADLRALFPEPL
                     VWSDSGTMPGQEAAADAIATVLAERRGENQERFYVDTPLAASLVNAETIIADAAGEKV
                     PAVIAPNPGLAPPGTIGEPPTSLPAPRVPQDG"
     gene            complement(1229..1849)
                     /locus_tag="VFC06_00170"
     CDS             complement(1229..1849)
                     /locus_tag="VFC06_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80352.1"
                     /translation="MAGLPGSIPSLIPAGPDEQVRRMRDVERRIDELGPSVARSFKPV
                     LDDLTAKQATLDAQGVTLAAQVATLTDLVANMIKPEVDHAWAQNFAITTTPTDFASVT
                     FTTPAGYTRALIAAVATGLGYNSTASTDWLYVTPWISGVSGLNEVYAGVDAGHGVSLA
                     SPWFDLLTGLTTGQVVTINVEMHTGTTWPANAGNVARIDAQVTFLR"
     gene            complement(1851..3176)
                     /locus_tag="VFC06_00175"
     CDS             complement(1851..3176)
                     /locus_tag="VFC06_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80353.1"
                     /translation="MPINPGDPGPTVWANGVDAALAAKLDANLTAHTGVTIADQAGDV
                     VSQYGTNETGQRWDIRVGTAATPTAGGGPSVKIAQTEKVTNLATQMGGNAVANEHNAA
                     LSVAALGLTGSLAQTNAVMIIANGAPTGTDVCALGSSGMVTGGTGTGFGAYLQGRRNV
                     PGASAIGAEIRVQNESGADGTANPNGMSNTVGVWVTTAGTGGFKSGAGVSLGTADATQ
                     FIHGYHTTAGAISDCAFRDESGSYASFSSQGTHNIGVDLNGAYSSYALRFTPKTDAAG
                     GISFGNDAFLYRSAAGVLTLNGALAVTGKVSSGAAKPHAEFTATVSLANATQGNIGVM
                     TVDAANTPDTTMVTSPSAGTLTFTNPGLYAITAAVNFQAVVTGESWASINTPSGVISR
                     SHATGRDVDGRVGHRHTVGPGGGTVTIDVFQTSGATRTVLTRVAVTRIA"
     gene            complement(3180..>3961)
                     /locus_tag="VFC06_00180"
     CDS             complement(3180..>3961)
                     /locus_tag="VFC06_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80354.1"
                     /translation="DRAYLRADRKTVYSALTELSACLGGPEWTAEWEWQHSPERITPV
                     FYVGDRVGRAVTPGLAPTATFQAPGCIIDAVMVEDYSAGKGANSVIAYSSAAAGVVPT
                     SLAQVAPDDGRPTFETDIQPSTSITDIATLTGHAQSALASLAPGGSALAMSANVSTAP
                     PLGSAWVLGDDVGYIIGGLEVDIHKHLVDVYGDIWWPDDIWHDHWGVVTGQQMVLVNP
                     DGRESVPAFPGGISGVMRSIGWELTLDGVQVVTPILASIGA"
BASE COUNT          653 a         1279 c         1334 g          695 t
ORIGIN      
        1 gcagcgatca tggcggtttc gacagttcgc tggttgtcgg ccttcagctc aaggcggtga
       61 gcctcagcga tcgcgtctgc tcgatgctcg gaggcgaacc gctcggcgtt gatgatttcg
      121 gcactcttga gttggagttc agcccagtcc cggacggagg tgatctcacg ttcgagtgtt
      181 tcgatggtgc gggcggtcgg gtctgggatc ggtcgcacgt cgccggagtt ggacagggcg
      241 tcggtcattg gttcctcctg gtcggcgttc gggtgaaggt ccacgcgggt cgttgacttg
      301 gcgctctgtg tcgtctactg tcgccatatg acgtcaacac ggctgatcac gatccgcgaa
      361 gctgcggcgc tctacagggt cagcgaacgc accatccagc gttggatcgc cagcggtgaa
      421 ctcaagtcgg aacgccccgg tgggcgtacc gtgagagtgg tagtggagga gacggaatca
      481 tgaggtcgcc agtgcgggtg atcgtctggg ctgtcgcgct cattgccctc ctcggaggcg
      541 ctaccgggtc cacggcagat gcacggatcg agccgcttcc ggtcggccac tactcgaccg
      601 tcgagatggc gtcggtcgac gaggtgtgcg cgggtcagcc gcgcatcgga cttgccgttg
      661 acatcgccgc atggcaggag gtccgttcgc tccgcgacct cgggcttccc gcgtgcgacg
      721 tcacctggcg gttcggctca ctcgtggact ggtcaggcaa ggcatgggcc gacgcgacca
      781 cggggcaggt gacgatcctc atcaacggcg accctgccaa ctacgcgggc gacagcgacc
      841 ctgcgtgggc gatccgctcg aacatccggc acgagttcgg gcacgccttg gtgttcatgg
      901 cgggatggac ctctggccag gaccatgctg acctgcgcgc cctgtttccc gagccgctgg
      961 tttggtcgga tagcgggacg atgcccggac aagaggccgc ggcggatgcc atagccaccg
     1021 ttctggccga gcgtcgcggc gagaatcagg agaggtttta cgtggacaca cccttggcgg
     1081 cgagcctcgt caacgcagaa acgatcatcg cggacgccgc tggcgagaag gtgcctgcgg
     1141 tcatcgcgcc caaccccgga ctagcacccc ccggcactat cggcgaacct ccgacctcgc
     1201 tgccggcacc gcgtgtgcct caggacggct agcgcaagaa ggttacctgt gcgtcgattc
     1261 gcgcgacatt cccggcgttc gccggccacg tggtaccagt gtgcatctcg acattgatcg
     1321 tcacgacctg accagtggtc agacccgtca aaaggtcaaa ccacggggaa gccagcgata
     1381 ccccgtggcc cgcgtcgaca cccgcatata cctcgtttag gccactcacg ccactgatcc
     1441 acggcgtcac atacagccag tccgtactag cggtcgagtt gtaacccaaa cccgtcgcca
     1501 cggccgcgat cagtgctcgc gtatagcctg ccggagtcgt gaaggtcacc gacgcaaagt
     1561 ccgtgggggt ggttgtgatc gcgaagttct gggcccacgc atggtcgact tcgggcttga
     1621 tcatgttcgc taccaggtcc gtgagcgtcg ccacctgcgc cgcgagggtc acaccctgcg
     1681 cgtcgagcgt cgcctgcttt gcggtgaggt cgtcgaggac cggcttgaac gaccgcgcca
     1741 cggacggacc aagctcgtcg atgcggcgct cgacgtcacg catgcgacgc acctgctcgt
     1801 caggacccgc ggggatgagg gacgggatgc tgcccggcag accggccatg tcaggcgatc
     1861 cgtgtgacgg cgacccgggt gagcaccgtg cgtgtggcac ccgaggtctg gaacacgtcg
     1921 atggtgacgg tgccgccacc gggaccgacc gtgtgtcggt gtccgacccg accgtctaca
     1981 tcccgaccgg tggcgtgcga gcgcgagatg acaccgctcg gggtgttgat cgaagcccac
     2041 gattcaccgg tgacaactgc ttgaaagttg acggctgctg ttatcgcgta aaggccaggg
     2101 ttggtgaaag tcaatgtccc cgccgagggc gaggtcacca tcgtcgtgtc aggcgtgttc
     2161 gccgcatcga cagtcatcac gccaatgttt ccttgcgtgg cgttcgccaa ggagactgtg
     2221 gccgtaaact ccgcgtgggg cttagccgcg ccgcttgaaa ctttccctgt gacagcaagg
     2281 gccccgttca gggtcaggac acccgcagcg gagcggtaca ggaatgcgtc gttgccgaag
     2341 gagatgcctc cagcggcgtc cgtcttcggc gtgaaccgga gcgcgtagct cgaatacgcc
     2401 ccgttcaggt ccaccccgat gttgtgcgtc ccctgggagg aaaaggatgc gtatgaaccg
     2461 gactcgtcac ggaacgcgca gtccgagatg gcaccggcgg tcgtgtggta gccatggatg
     2521 aactgcgtcg catctgcggt gcccagagac acacctgcgc cagacttgaa cccgccagtg
     2581 cccgccgtgg taacccagac gccgaccgta ttactcatgc cattcgggtt ggctgtgccg
     2641 tccgcgcccg actcgttctg cacgcgaatc tcggcaccaa tggcactggc tcctgggacg
     2701 ttgcggcggc cctgtaggta ggcaccgaag cccgtaccgg tgccgcccgt gaccatgccc
     2761 gacgagccca gcgcgcagac gtccgtcccg gtaggtgcac cgttggcgat tatcatgacg
     2821 gcattcgtct gcgcgagcga acccgtcagt ccgagggcgg cgaccgacag ggcagcgttg
     2881 tgctcgttcg cgacggcgtt gccgcccatc tgcgtagcca ggttcgtgac cttctcggtc
     2941 tgcgcgatct tcacagacgg gccaccgcca gcggtcggcg tggcggccgt accgacccga
     3001 atgtcccacc gctgcccggt ctcgttcgtc ccgtactgcg acacgacgtc gcccgcctgg
     3061 tccgcgatgg tcacgccagt gtgagccgtc agattcgcgt caagcttcgc tgcgagggcg
     3121 gcgtcgacgc cgttggccca gacggtcggg ccgggatcgc caggattgat gggcatgtgt
     3181 caggctccta tcgaggcaag aatcggcgtt acgacctgca cgccgtcgag agtcagttcc
     3241 cagccaatcg accgcatgac gccggagatc ccgccgggga acgcgggcac ggactccctg
     3301 ccgtcggggt tgacgaggac catctgctga cccgtgacga caccccagtg gtcatgccag
     3361 atgtcatccg gccaccagat gtcgccgtag acgtccacga ggtgcttgtg gatgtcgacc
     3421 tcgagcccgc cgatgatgta accgacgtcg tccccgagga cccaggcgct gccgagcggc
     3481 ggggccgtgg agacgtttgc ggacattgcc agggccgacc cgccaggggc gagggacgcg
     3541 agggccgact gtgcgtggcc ggtcagggtg gcgatatccg tgatggacgt cgacggctga
     3601 atgtccgtct cgaacgtcgg ccgcccgtca tccggggcga cctgcgcgag ggatgtcggg
     3661 acgacgccag cggctgcgct gctgtaggcg atgaccgagt tcgcaccctt acccgccgag
     3721 tagtcctcga ccatgacggc atcgatgatg caacccgggg cctggaacgt cgccgtgggc
     3781 gcgaggccgg gggtgacggc ccgcccgaca cgatcgccca cgtagaacac gggcgtgatg
     3841 cgctccggcg agtgctgcca ctcccactcg gccgtccact cggggccacc gaggcacgcg
     3901 ctcagctcgg tcagcgcgct gtagaccgtc ttccggtcgg cgcgcaggta cgcccggtca
     3961 c
//
LOCUS       DASRKH010000012         6245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_17673, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000012 DASRKH010000000
VERSION     DASRKH010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 6245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6245
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17673"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VFC06_00185"
     CDS             complement(<1..363)
                     /locus_tag="VFC06_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZL80355.1"
                     /translation="MPLPVAPGTPVEQLTRALIDIPSVSGDEARIADEVESALRVCSH
                     LDVTRLGDAIVARTTLGRAERVIIAGHLDTVPIKDNVPSKLSADGTEVIGRGAVDMKA
                     GVAVQLALAVELVASTRDI"
     gene            complement(373..702)
                     /locus_tag="VFC06_00190"
     CDS             complement(373..702)
                     /locus_tag="VFC06_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80356.1"
                     /translation="MEGREKDDEEVPRPPRRDERLPPPWFERAEGAAPREPDGDIPRL
                     RLSWPRAIGLLALVLLIIGVPLDFAFVYFSVDNALDRTPMTLGFVWAVVIEVLAVWGL
                     IRLLTRR"
     gene            743..1684
                     /gene="dapD"
                     /locus_tag="VFC06_00195"
     CDS             743..1684
                     /gene="dapD"
                     /locus_tag="VFC06_00195"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HZL80357.1"
                     /translation="MKAHGYGLATLDGSGNPLDTWYPSPSLGAAPERPNPPQQLEAGV
                     RSDVLRQISTDLLLIEIDTDEAPETVADAYLRLHLLSHRLVKPHGINLDGIFAILPNV
                     VWTSAGPCAIEGFEATRGRFRAFGQQLTVFGIDKFPRMVDYVLPSGVRIADADRVRLG
                     AYLAEGTTVMHEGFVNFNAGTLGTSMVEGRISSGVTVGNGSDIGGGASIMGTLSGGGK
                     EVITIGERSLLGANAGLGIPLGDDCIVESGLYVTAGSKVRIVGATDDDGEPVVVKARE
                     LAGRNNLLFRRNSLRGGLEVAAREGTGIELNAALHAN"
     gene            1687..2550
                     /locus_tag="VFC06_00200"
     CDS             1687..2550
                     /locus_tag="VFC06_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019481636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80358.1"
                     /translation="MSRTGAAVAVAAFVAVGADAVYWVPGLLNGDRQRVPTETCTVTQ
                     DDIGHSLTAEQADNAALIAAVGSREGFGVGGITVALATAIQESSLRNLDYGDRDSLGL
                     FQQRPSQGWGSAEQIADPYYSTATFYAALLRVDGWEGLAVTDAAQAVQRSGFPFAYAD
                     HEVEARAWALAFTGGGGLITCSVAGPPVATPQQFADRLAADFGTGVNAVDVVGTEGDA
                     TILGVRPGADSPEARHALSAWAIATASVTGVTWVEGDAGVVATDGSVTSIDPAAALQD
                     YAGIRVAVATR"
     gene            complement(2547..3836)
                     /locus_tag="VFC06_00205"
     CDS             complement(2547..3836)
                     /locus_tag="VFC06_00205"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HZL80359.1"
                     /translation="MTEVKDVQFTVDGSTSKLEIERASVGNDGLSIGHLLNDTGLVTV
                     DPGFMNTASCESAITYIDGDKGVLRYRGYPIEQLADHSTFLEVAYLLIHGELPTESEL
                     TAFNNRVARHMHVHEGIKTFLGAFPRNAHPMAVLSGAVSALSTFYPESVGRGEYEIEL
                     ATVQLLAKTAVVISYLQRRVTGGELIEPRPGGLYVDEFMRVAFSGQDGELDIDPTVRR
                     ALDLLLILHADHEQNCSTSTVRIVGSSQANIYAAVSAGIGALSGPLHGGANEAALKMF
                     DQIKESGDTVEQFVAKVKDKAEGARLMGFGHRVYKSYDPRGTVVKKIADQVLDQLGGN
                     NETFDMAKRLEEIALHDDYFIERKLYPNVDFYTGLVYSAMKFPVPMFTPLFALGRMPG
                     WIAHYREMMNDPRTKIGRPRQIYTGVVERDYVAMDLR"
     gene            complement(3895..5016)
                     /gene="dapC"
                     /locus_tag="VFC06_00210"
     CDS             complement(3895..5016)
                     /gene="dapC"
                     /locus_tag="VFC06_00210"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HZL80360.1"
                     /translation="MGLNTSSLPDFPWDSLTPFIERASAHPDGIVNLSVGTPADPTPL
                     VVREALTRAADAPGYPATHGTPALREAVAHWFARRRGVPDVDPAGVMPTIGSKELVAF
                     LPALLGLGVGDVVVHPSAAYPTYDVGARLAGATPFPCDDVAEWEDNRAVKLVWVNSPS
                     NPTGTVMGAAELRATVEAARAIGAVVASDECYAELPWEEPWVSDGVPSLLDPVVAGAD
                     HTGLLALYSLSKQSNLAGYRAAFAAGDPALIATLLETRKHAGMMMPAPVQAAMVAALT
                     DDAHVAAQREVYRARRELLSAALRDAGFTIDGSAAGLYLWCTRGEDCWQTISWLADRG
                     IVAAPGAFYGQAGTQHVRVALTASDERIASAARRLVNKQ"
     gene            complement(5026..5346)
                     /gene="fdxA"
                     /locus_tag="VFC06_00215"
     CDS             complement(5026..5346)
                     /gene="fdxA"
                     /locus_tag="VFC06_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HZL80361.1"
                     /translation="MTYVIALPCVDLKDKACIDECPVDCIYEGERMLYIHPDECVDCG
                     ACEPVCPVEAIYYEDDVPDKWSDYYAANVEFFSEIGSPGGAAKMGLIAHDHDLIKALP
                     PQIV"
     gene            complement(5420..6046)
                     /locus_tag="VFC06_00220"
     CDS             complement(5420..6046)
                     /locus_tag="VFC06_00220"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HZL80362.1"
                     /translation="MANEKIRNQNLQHAADKISGVIVAPGANFDLTKVLSPITIADGY
                     VSAGVINNGIHTTGVGGGLSQMATTSYNAAYFAGFDLLDHRQHSVWFTRYPAGRESTI
                     YTGQINMVFKNDTPYAAIMNSYIEDNRLHVDIWSTPYYRVETEATPHMNVVRPGTTVI
                     TDPDCVPSGAGQSGFKITNYRRVYLGDKLVKDEADTWTYKPDNAIECA"
BASE COUNT         1090 a         2066 c         2009 g         1080 t
ORIGIN      
        1 gatgtcccgc gtcgacgcaa ccaactcgac cgcgagcgcg agctgcacgg ccacgccagc
       61 cttcatatcg accgctccgc gcccgatgac ctccgtgccg tccgccgaca gtttcgacgg
      121 cacgttgtct ttgatgggca cggtgtcgag gtggccggcg atgatcaccc tttcggccct
      181 gcccagcgtg gtgcgggcca cgatcgcgtc gccgagccgg gtgacgtcca ggtggctgca
      241 gacgcgcaac gccgactcca cctcgtcggc aattcgcgcc tcgtcgccgg agaccgaggg
      301 aatgtcgatg agcgccctcg tgagttgctc gaccggggta ccgggggcga cgggaagggg
      361 catggctcga tcctatcggc gcgtcagcag ccgaatcagg ccccacacgg caagcacctc
      421 gatcaccaca gcccagacga atccgagggt catcggcgtg cgatccagcg cattgtccac
      481 agagaagtag acgaacgcga agtcgagtgg tacgccgatg atcagaagta ccagtgccaa
      541 caagccgatc gcgcgcggcc aggacaggcg aagccgagga atatccccat ccggctcccg
      601 tggcgcggcg ccctcggccc gctcaaacca cggcggcggg aggcgttcat cgcggcgcgg
      661 cggccgcgga acttcctcgt cgtctttctc gcgcccctcc atgtccacac ggtagtcccg
      721 gcgtgcccgt aggcttacgg ccatgaaagc gcacggctac ggactcgcaa cgctcgacgg
      781 cagcggaaat cccctcgata cctggtatcc ctccccctcc cttggcgcgg cgcccgaaag
      841 gcccaaccca cctcaacaac tcgaggcggg ggtgcgatcc gacgtattac gccagatttc
      901 gaccgacctg ttgctcatcg agatcgacac cgacgaggcg ccggagaccg tcgccgacgc
      961 gtatctacgc ctccacctgc tgagccaccg actcgtcaag ccgcacggca tcaacctcga
     1021 tggcatcttc gcgatccttc ccaacgtggt gtggacatcg gcaggcccgt gtgcgatcga
     1081 aggtttcgag gcgactcgcg gccgcttccg cgccttcggc cagcaattga cggtcttcgg
     1141 aatcgacaag ttcccccgca tggtggacta cgtgctcccg tcgggggtgc gcatcgccga
     1201 tgccgaccga gtccgcctgg gtgcctactt ggccgagggc acgaccgtga tgcacgaggg
     1261 attcgtcaac ttcaacgccg gcacgctcgg cacctccatg gtcgagggac gcatctcgtc
     1321 cggcgtgacc gttggcaacg ggtccgacat cggcggcggc gcgtccatca tgggcaccct
     1381 gtccggcggc ggtaaggaag tcatcacgat cggcgagcgg tcgctactgg gcgcaaacgc
     1441 gggcctcggc atcccccttg gcgacgactg cattgtcgaa tcgggcctct acgtgacggc
     1501 cgggtccaag gtgcggatcg tcggcgcgac ggacgacgac ggcgaacctg tggtggtcaa
     1561 ggcgcgtgaa ctcgcgggca ggaacaacct gctgttccgc cgcaactccc tgcgcggcgg
     1621 cctcgaagtg gcggctcgcg agggcaccgg catcgaactc aacgccgccc tgcacgccaa
     1681 ctagcgatgt cgcgaacggg cgcggcggtt gcggtggccg cgttcgtcgc agtcggtgcc
     1741 gacgccgtct attgggttcc gggactcctg aacggggacc gtcaacgcgt tcccaccgaa
     1801 acgtgcaccg tcactcagga cgacatcggc cattcgctga ccgccgagca ggctgacaat
     1861 gcggcgttga tcgcggccgt cggctcgcgc gagggatttg gcgtcggcgg cattaccgtg
     1921 gccttggcca ccgccatcca ggagtcaagc ctgcgcaacc tcgactatgg cgatcgcgat
     1981 tcgcttggac tgttccagca gcgtccatct cagggatggg gatcggcgga gcagatcgcg
     2041 gacccgtact actcgaccgc gacgttctat gcggcattgc tgagagtcga cggttgggaa
     2101 ggcctggcgg tcacggacgc cgcgcaagca gtccagagat cgggctttcc cttcgcgtac
     2161 gccgaccacg aggtcgaggc gcgcgcttgg gcgctcgcct tcaccggtgg cggcggactc
     2221 atcacctgct ccgtcgcggg tccccctgtg gccacgccgc agcagttcgc cgaccgactc
     2281 gccgccgact tcggcacagg ggtgaacgcc gttgacgtcg tggggaccga gggggatgcc
     2341 acgatcctgg gcgtccggcc cggagcagac tcgcctgagg cgcgacacgc gctttccgcg
     2401 tgggcgattg ccacggcatc tgtcaccggg gtcacgtggg tggagggaga cgccggggtg
     2461 gttgccacgg acggctcggt cacgtcgata gatcccgcgg ccgcgctgca ggactacgca
     2521 ggcatccggg tcgctgtcgc gacgcgctag cgcagatcca tcgcgacata gtcgcgctcg
     2581 acgacaccgg tgtagatctg gcgcggacgg ccgatcttgg tgcggggatc gttcatcatc
     2641 tcgcggtagt gggcgatcca acccggcatg cgacccagcg cgaatagcgg ggtgaacatc
     2701 ggcaccggga acttcatggc cgaatagacc aagccggtgt agaagtcgac attggggtac
     2761 agcttgcgct cgatgaagta gtcgtcatgc agtgcgatct cttcgagtcg cttcgccatg
     2821 tcgaacgtct cgttgttgcc gcccaactgg tcgagcacct ggtcggcgat cttcttgacg
     2881 accgtgccgc gcggatcgta ggacttgtac acgcggtgac cgaaccccat gaggcgcgca
     2941 ccctcggcct tgtccttgac cttggctacg aactgctcga ccgtgtcgcc cgactccttg
     3001 atctggtcga acatcttgag cgccgcctcg ttggcaccgc cgtgcagagg tccggacagt
     3061 gcgccgattc ccgccgagac cgcggcgtag atattggcct gggacgagcc gacgatgcgc
     3121 acggtcgacg tggagcagtt ctgctcgtgg tcggcgtgaa ggatgaggag gaggtcgagc
     3181 gcgcgacgca cggtgggatc gatgtcgagt tcgccgtcct ggcccgagaa cgcgacacgc
     3241 atgaactcat ccacgtaaag gccgccgggg cgcggctcga tcaactcgcc accggtgacg
     3301 cgacgctgca ggtaggaaat caccacagcg gtcttggcca gcaactggac cgtcgccaac
     3361 tcgatctcgt actcgcctcg ccccaccgac tcggggtaga aagtgctcag cgcgctcacg
     3421 gccccggaca agacggccat cgggtgcgcg ttgcggggga aggcccccag gaacgtcttg
     3481 attccctcgt gcacgtgcat gtggcgcgcc acgcggttat tgaaggcggt cagctcggac
     3541 tcggtcggca actctccgtg aatcaacagg taggcgactt cgaggaacgt ggagtggtcg
     3601 gcgagctgct cgatcgggta gccgcggtac cgcagcacgc ccttgtcgcc gtcaatgtat
     3661 gtaatggcgc tctcgcacga ggcggtgttc atgaacccgg ggtcgacggt aacgagaccg
     3721 gtatcgttca gcaggtggcc aatcgagagg ccatcattgc cgaccgaggc gcgctcgatt
     3781 tccagcttgc tcgtggaacc atccaccgtg aactggacgt ccttgacctc agtcatgttt
     3841 cctccttgga agcgcaccgc ccactgtgac acgtacccgc gcttgggaac aagtttactg
     3901 tttgttgacg agcctacgag ccgcagaggc gatgcgctca tcgctcgcgg tcagcgctac
     3961 gcgcacgtgc tgggtgccgg cttgcccata gaacgcgccc ggggcggcca cgatgccgcg
     4021 gtcggccagc caactgatcg tctgccagca gtcctcgcct cgggtgcacc ataggtagag
     4081 acccgctgcc gatccgtcga tcgtgaatcc cgcgtcgcgc aaggcggccg acagcagctc
     4141 gcgacgtgcc cgatacactt cacgctgcgc ggccacgtgg gcatcgtccg tcagtgccgc
     4201 caccatcgcc gcctggaccg gagccggcat catcatgccc gcgtgcttgc gcgtctccag
     4261 caaggtggca atcagtgcgg gatcgccggc agcgaaggcc gccctgtacc cggccagatt
     4321 ggactgcttg gacagcgagt acaacgccag cagacccgtg tgatctgccc ccgcgaccac
     4381 cggatccaag agagacggga cgccgtcgct cacccacggc tcctcccacg gcaattccgc
     4441 gtagcactcg tcagaagcga cgaccgcgcc gatcgcgcga gctgcctcta ccgtggcgcg
     4501 cagctccgct gcgcccatca cggtgcctgt gggattggac ggggagttca cccacacgag
     4561 cttgaccgcg cggttgtctt cccattccgc gacgtcatcg caaggaaacg gcgtcgcccc
     4621 ggcaagccgg gcgcccacgt cgtatgtggg atacgcggcg gacgggtgca ccaccacgtc
     4681 tccaacgccc agccccagca gcgccggaag gaaggcaacg agctccttgg aaccgatcgt
     4741 cggcatgact ccggctggat ccacgtccgg cactcccctg cgccgcgcga accagtgcgc
     4801 cacggcctca cgcaacgcgg gcgtgccgtg cgtcgccggg tatccgggcg catcggccgc
     4861 gcgcgtcagg gcttcgcgca ccaccagcgg cgtcgggtca gcgggggtac ctaccgacag
     4921 gttgacgatg ccatcgggat gcgcggacgc ccgttcgatg aagggcgtca gagagtccca
     4981 ggggaagtcg ggtagcgacg atgtgttcag gcccaccagg gaccctcaga cgatctgcgg
     5041 cggaagggcc ttgatgaggt cgtggtcgtg cgcgatcagg cccattttcg cggcgccgcc
     5101 gggcgagccg atctccgaga agaattcgac gttggccgcg tagtagtcgg accacttgtc
     5161 gggcacgtcg tcctcgtagt agatggcctc gacggggcac actggctcac acgccccaca
     5221 gtcaacgcac tcatcagggt ggatgtagag catgcgctca ccctcataaa tgcagtcaac
     5281 gggacactcg tcgatgcagg ccttgtcttt gaggtctaca cacggcaagg cgatgacgta
     5341 cgtcacgagc gctccctttt ctaggacttg gagagggact tcatcttaac gcggcctgac
     5401 ggccgccatt ccgcttcgcc tacgcgcact cgatcgcatt gtcgggcttg tatgtccacg
     5461 tatcggcctc gtccttgacc aacttgtcac caaggtacac gcgccgatag ttggtgatct
     5521 tgaatccaga ctgacccgcc ccggagggta cacagtccgg atcggtgatc accgtcgtac
     5581 cgggccgcac gacgttcatg tggggagtcg cttccgtctc cacccggtag tacggggtgg
     5641 accagatgtc gacgtgaagc ctgttgtcct cgatatacga gttcatgatt gccgcatagg
     5701 gcgtgtcgtt cttgaacacc atgttgatct ggccggtata gatcgtcgac tcacgcccgg
     5761 cggggtaccg cgtgaaccac acgctgtgtt ggcggtgatc gaggagatcg aagcctgcga
     5821 agtatgccgc gttgtagctt gtggtcgcca tctggctgag cccgccgccc actcccgtgg
     5881 tgtggatgcc gttgttgatc acacccgccg agacataccc gtccgcgata gtgatggggc
     5941 tcagcacctt tgtcaggtcg aagttggcgc cgggagccac gatgacgccg gaaatcttgt
     6001 cggccgcgtg ttgcaggttc tgattcctga tcttctcgtt cgccagcgga aaacgtcaac
     6061 atccgagact tcaccaccag gatctcggcc ttcgacacgc cgctgacgaa cgagaagatc
     6121 aggaaccaaa acctgcaaca cgccgccgac aagatttccg gcgtcatcct ggctcccggc
     6181 gccacgttcg acctcaccaa ggtgctgagc cccatcacta tcgcggacgg gtatgtctcg
     6241 gcggg
//
LOCUS       DASRKH010000013         2602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_17672, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000013 DASRKH010000000
VERSION     DASRKH010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17672"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VFC06_00225"
     CDS             <1..357
                     /locus_tag="VFC06_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HZL80363.1"
                     /translation="VDASALLAFLQGETGADTVEEVLTRGSDCSAVNWSEVAQKVGAA
                     GGDWPLAAALLSSYGVRVRDATVDDAERAAALWRSGSGLSLADRFCLALGDRLNAMIW
                     TADTAWGNTDRIRQVR"
     gene            complement(425..894)
                     /locus_tag="VFC06_00230"
                     /pseudo
     CDS             complement(425..894)
                     /locus_tag="VFC06_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012751128.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            1293..2183
                     /locus_tag="VFC06_00235"
     CDS             1293..2183
                     /locus_tag="VFC06_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008726798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZL80364.1"
                     /translation="MIETTKLTKSYGTARGIVDVDMTVLEGEIYGFIGPNGAGKSTTI
                     RTLLGLIHPTSGSATVFGKDITRFGPEIRQEVGYLPSEVFYYDDMRAIDLLKYSVSFY
                     AKDGKAAEKRIRDLAEVFDLDLKKKIDDMSYGNKKKVGIIQGLAHQPRLIILDEPTGG
                     LDPLIQQQFFHLLKEENVKGATILFSSHILGEVQRLCDRVAIIKDGSIIKVEQIKTLL
                     ENQTKRFKLELAGPPDPARFTIDGVSDLAVTNHSVSFLYRGHINAVTHLLSGLDLVDL
                     LVEEPDLEEIFLHYYRKDAP"
     gene            2180..>2602
                     /locus_tag="VFC06_00240"
     CDS             2180..>2602
                     /locus_tag="VFC06_00240"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZL80365.1"
                     /translation="MNIFLRELRANRRSTITWTVSLSTIVVVFMGLYPAFSADVDTTR
                     ELLAQFPEALRTALNLSLDNFFTIQGFFGYLLGFAVLAGGIQAMNLGTGIIAKEVSGK
                     TADFLLSKPVTRSTVVTAKLAAALTILVITNVGFALVSY"
BASE COUNT          467 a          878 c          855 g          402 t
ORIGIN      
        1 gtcgacgcct cggcgctgtt ggccttcctc cagggcgaga caggggcgga cacggttgag
       61 gaggtgctga cgcgcggctc cgactgttcc gccgtgaact ggtcagaggt cgcgcagaag
      121 gtgggggcgg cgggcggcga ctggccgctg gccgcggcac tgttgtccag ttacggcgtc
      181 cgggtccgtg acgcgaccgt cgacgacgcc gagcgagcgg ccgcattgtg gcgcagcggg
      241 tccgggctgt cgctcgcgga caggttctgc ctggcgctcg gcgaccgact gaacgcgatg
      301 atctggacgg ccgacacggc gtggggaaac actgaccgca tccggcaggt tcgctgagga
      361 gatcacccgt tcagggtggg ccggcacgcc tcagggaggg gtgaacaggt cttcgatgcg
      421 gatctcacaa cactgcgagt ccctccaccg gggagagtcg ggcggcccga cgggacggac
      481 ccaccgaggc cagtaacccc gccaccacgg ccaccgccac cacagcggcg agttccagcc
      541 acggcacggt cggcaccagc cggcccgccg cgctgccctc cgccaccagg ttcccgagca
      601 gcgactcggc gcccatccag ccgtagaaca ccccgagcgc gctgcccagc agggccgcga
      661 tcccggcgat cagcaccgcc tcggtggcga gcatcgtgcg cagcccgcgc cgggtcagcc
      721 ccagcgcccg catcagcgag ttctcccgcg tccgctcgat cgtggacagg gacagcgtgt
      781 tcgccacccc gatcagcgcg atcagcacgg acaccgcgag gaggccggtc accacgagca
      841 gcatcgtgtc gatgatctgc tggtacatca ggcgctccgc cagcgccccg agacctgcga
      901 cgccgacacc ccggcagcct ccgccacggc gggcaccagg tcggtgacgg tgtcgaggtc
      961 cgtgccggcc gcggtgcgcc ctcctgcagc accgcgttca ccccggccgg ggactccgaa
     1021 gagggtgagc agcgccgtcg ccagcacgtc ggtgatgacc gggtgggcgc ggaagaagct
     1081 gtccaggtgc cgcagggggt tgaggccgcc gggcaccgtc gcgagtgcgc gcggttgcgt
     1141 gtccaggctg cccctagcgt tcaggctagc cgcgcggact cgagggtgtt cgtagggaca
     1201 ctccgctgta cctcgcccat aggcacgacg gcaggcagat actgggtgac gtcaatcggt
     1261 ggcgtggaac ggtcaacgga gcagagatgt atgtgatcga gacgacgaag ctgaccaagt
     1321 cctacggcac ggcccgcggc atcgtcgacg tcgacatgac cgtgctcgaa ggcgagatct
     1381 acggcttcat cgggcccaac ggcgccggga agtcaacgac gataagaacg ctcctcgggc
     1441 tcatccaccc cacgagcggt tcggcgacgg tcttcgggaa ggacatcacg agattcggcc
     1501 ccgagatccg gcaggaggtc ggctaccttc cttcggaggt cttctactac gacgacatgc
     1561 gggcgatcga tctcctcaaa tattcggtga gtttctacgc caaggacgga aaggccgccg
     1621 agaaacggat ccgggacctc gccgaggtgt tcgacctcga tctcaagaag aagatcgacg
     1681 acatgagtta cggcaacaag aagaaggtcg ggatcatcca ggggctcgcc caccagccca
     1741 gactcatcat cctggatgag ccgaccggcg ggctggaccc actcatccag cagcagttct
     1801 tccatctcct gaaggaggag aacgtgaagg gcgccacgat cctcttctcc tcgcacatcc
     1861 tgggcgaggt acagcggctc tgtgaccggg tggcgatcat caaggatggc tcgatcatca
     1921 aggtcgaaca gatcaagacc ctgctcgaga accagaccaa gcgcttcaag ctcgaactgg
     1981 ccggcccgcc ggatccggct cggttcacca tcgacggcgt cagtgatctg gcggtgacca
     2041 accactcggt gagcttcctg taccgcgggc acatcaacgc cgtcacgcac ctgctctccg
     2101 ggctggacct cgtggacctg ctggtggagg agccggacct cgaggagatc ttcctgcact
     2161 actaccgcaa ggacgcgccg tgaacatctt cctgcgcgaa cttcgggcca atcggaggtc
     2221 cacgattacc tggaccgtct cgctgagcac catcgtcgtg gtgttcatgg gcctgtaccc
     2281 ggcgttttcg gccgacgtcg acaccacccg ggaactcctc gcgcagttcc cggaggccct
     2341 gcgaaccgcg ctcaacctct ccctggacaa ctttttcacg atccagggct tcttcggcta
     2401 cctgctgggc ttcgccgtcc tggcgggcgg gatccaggcc atgaacctcg ggacgggcat
     2461 catcgcgaag gaggtgtccg gcaagaccgc ggacttcctg ctgtccaagc cggtcacccg
     2521 aagcaccgtg gtgaccgcga aactggcggc ggcgctaacg atcctcgtca tcaccaacgt
     2581 gggcttcgcc ctggtgtcgt ac
//
LOCUS       DASRKH010000014         2240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_22015, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000014 DASRKH010000000
VERSION     DASRKH010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22015"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /gene="carB"
                     /locus_tag="VFC06_00245"
     CDS             complement(<1..708)
                     /gene="carB"
                     /locus_tag="VFC06_00245"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882533.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HZL80366.1"
                     /translation="MPRRDDIHTVMVIGSGPIVIGQACEFDYSGTQACRVLKAEGLRV
                     VLLNSNPATIMTDPEFADATYVEPISTDVIERIIEKERPDALLATLGGQTALNAAIAV
                     SEAGILERYNVELIGASLEAIHLGENREMFKGVVERCGAESARSHICHTMDELVAAAA
                     DLSYPVVVRPSFTMGGLGSGFAYDEDDLRRIGGAGLHYSPSTEVLLEESILGWKEYEL
                     ELMRDKHDNVVVVCSIEN"
     gene            complement(708..1856)
                     /gene="carA"
                     /locus_tag="VFC06_00250"
     CDS             complement(708..1856)
                     /gene="carA"
                     /locus_tag="VFC06_00250"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HZL80367.1"
                     /translation="MSNEPAVLVLENGDVYRGEAYGARGETLGEIVFNTGMTGYQETL
                     TDPSYYQQIVVMTAPHIGNTGMNYEDAESRRIWVAGFVMREPARRPSNWRSQRPLGDD
                     LTEQGIVSMSGIDTRKLTRTLRTFGSMRGGIFSGEALADDADLLARVLAAEPMAGAHL
                     ADAVSIDGDYVIEPPDGVEQVAHVVAVDLGIKAMTPELMAQRGIKVTVVPSSWTAAQI
                     LAREPDGVFFSNGPGDPGVADRSVEVVRGILDARVPLFGICFGNQLLGRALGYGTYKL
                     RFGHRGINQPVMDRTTGKVEVTSHNHGFAVDAPLDGTSESPNGYGRVHVSHVCLNDDV
                     VEGLECLDAPAFSVQYHPEAAAGPHDASYLFDRFLDLMHGRRTLEGTR"
     gene            complement(1853..>2240)
                     /locus_tag="VFC06_00255"
     CDS             complement(1853..>2240)
                     /locus_tag="VFC06_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018773141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HZL80368.1"
                     /translation="PHPSEDKDCEWEAAAAGMTGLETALGIVQSVMVDSGLMTWRDVA
                     RVMSTTPAAIGRVSQWHGRPIAVGEPANLTLVDPRAEHTVDPSTHASRSRNSPYAGRV
                     LPGRAVATFLRGTPTFLDPRFEGAFA"
BASE COUNT          367 a          739 c          749 g          385 t
ORIGIN      
        1 gttctcgatg gagcacacca ccacgacgtt gtcgtgcttg tcgcgcatca gctcgagttc
       61 gtattccttc cacccgagga tcgactcctc aagcagcacc tctgtggagg gcgaatagtg
      121 caggccagcc ccgccgatgc gtcgcaggtc atcctcgtcg taggcgaagc ccgaacctag
      181 gccgcccatc gtgaaggacg ggcgcaccac gacggggtac gacaggtccg ctgcggcggc
      241 aacgagttcg tccatcgtgt ggcaaatgtg cgagcgcgcg ctctctgctc cgcagcgctc
      301 cacgacgccc ttgaacatct cacggttctc gcccaggtgg atggcttcca ggctggcgcc
      361 gataagttcc acgttgtagc gctcaagaat gcccgcctcg gacacggcga tcgcggcatt
      421 cagcgcggtt tgcccgccaa gagtggcgag cagcgcatcg gggcgttctt tctcgatgat
      481 gcgttcgatg acatcggtcg agatgggctc gacgtaggta gcgtcggcga actcggggtc
      541 cgtcatgatt gtggcggggt tcgagttgag cagcaccacg cgcaggccct cggccttgag
      601 tacgcggcac gcctgggttc ccgagtagtc aaactcgcac gcctggccga tgacgatcgg
      661 gccggagccg atcaccatca ctgtgtggat atcgtcgcgg cggggcacta gcgggtccct
      721 tccaaggtgc gccgaccgtg catcaggtcg aggaatcggt caaagagata cgaggcgtcg
      781 tgcgggcccg ctgcggcctc ggggtggtac tggacggaga acgcgggagc gtcgaggcac
      841 tcgagcccct cgacgacgtc gtcgttgagg catacgtggc tgacgtgcac gcgaccgtag
      901 ccgttcggcg actcgctcgt gccgtccaac ggcgcatcga ccgcgaatcc gtggttgtgg
      961 ctcgtgacct ccaccttgcc ggtggtccgg tccatcacgg gctggttgat gccgcggtgg
     1021 ccaaagcgga gcttgtaggt gccgtagccg agcgcgcggc ccaacagctg attgccgaag
     1081 cagatgccga acaagggcac gcgagcgtcg agaatgccgc ggaccacctc aacggaccgg
     1141 tccgccacgc ccgggtcgcc gggcccgttg gagaagaaca cgccgtccgg ctcgcgagcc
     1201 agaatctgcg ccgcggtcca ggacgagggc accaccgtca ccttgatgcc gcgctgggcc
     1261 atgagttcgg gtgtcatcgc cttgatgccg aggtccacgg ccacgacgtg cgccacctgc
     1321 tcgacgccgt cgggcggctc gatgacgtag tcaccgtcga tggagacggc atcggcaagg
     1381 tgcgcccccg ccatcggctc ggcggccagc acccgggcca gcaagtcggc gtcgtccgcg
     1441 agggcctctc ccgagaagat ccccccacgc atcgatccga aggtgcgcaa ggtccgagtg
     1501 agcttgcggg tgtcgatccc cgacatgctg acgatgccct gctcggtgag gtcgtcgccg
     1561 agcggccgct gcgaacgcca gttgctgggt cgccgcgcgg gctcccgcat gacaaatccg
     1621 gccacccaga tgcggcgcga ttcggcgtcc tcgtagttca tcccggtatt gccgatgtga
     1681 ggtgccgtca tcaccacaat ctgctggtag tacgacgggt cggtgagggt ctcttggtag
     1741 ccggtcatgc cggtattgaa gacaatctcg ccgagagtct ctccgcgcgc cccatacgcc
     1801 tcgcctcggt acacgtcgcc gttctccagg acgagcaccg cgggttcgtt gctcacgcga
     1861 acgctccttc aaatcgaggg tcgagaaagg tgggggtgcc gcgcagaaat gtcgccaccg
     1921 ctcgaccggg caggaccctg ccagcgtacg gcgagttacg ggacctgctc gcatgcgtcg
     1981 aggggtcgac agtgtgttca gcacggggat ctaccagggt gaggttggcg ggttcgccca
     2041 ccgcgatggg tcgaccatgc cactgcgaca cccggccgat ggcggcgggc gtggtcgaca
     2101 tcacgcgtgc cacgtccctc cacgtcatca ggccggagtc caccatgacg ctctggacga
     2161 ttcccagcgc cgtctccagc cccgtcatgc cagccgcggc ggcctcccac tcgcagtcct
     2221 tgtcctcgct ggggtgcggg
//
LOCUS       DASRKH010000015         2063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_62029, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000015 DASRKH010000000
VERSION     DASRKH010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62029"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VFC06_00260"
     CDS             <1..204
                     /locus_tag="VFC06_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80369.1"
                     /translation="DPRPTWTVRVADLHENVDHTPYEGTEVTGRVRDVFLRGHRAVVD
                     GHLADELPQGVFLPCGTPDTSVV"
     gene            234..>2063
                     /gene="xdh"
                     /locus_tag="VFC06_00265"
     CDS             234..>2063
                     /gene="xdh"
                     /locus_tag="VFC06_00265"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0046415 - urate metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HZL80370.1"
                     /translation="MSIMVNGQAHDIPGAMTLMRYLREDLALSSVKHGCDDGSCGSCL
                     VVVDGKTVKACTRDVTTFDGRHVTTAEGLTERERAAIVSAYEQEGAVQCGFCIPGFVM
                     ATKALLDKSPEPGRDEVTKAIRSNVCRCTGYRMIEDAVLRSGRILRGEEEIPPPPEHI
                     RMSDRAPRLDAAPKVLGTGLYADDLRLDGMVHAKALRSAHPRAVVERIDVSRAAAHPD
                     CLAVLTARDVPENTIGHLVNDWDVFIAAGDTTRYVGDALALVVCEHEETLDEVIGLID
                     VAYTVLEPVTSPAQALAPGAPLIHPGGNVLTHEQVKRGDADAALAASAFVVTNTFHTP
                     WQEHAFLEPECAVAQSWGEGGVEVFTGGQSVYDERREISRMLGLPAERVHSHSMLVGG
                     GFGGKEDMSVQHHAALAAWHTGRPVKVKFSRQASLDYHVKRHPMDLEVTLGCDETGQL
                     TAAKVRIVADTGAYASLGGPVLQRAVTHAGGPYNYQNFDCEGLAVYTNNPVSGAFRGF
                     GVAQSDFALENSVNLLAAKAGIDPWEFRYRNAIRPGQEMPNGQIADEATGLVECLEAL
                     KPDWDADPAAGLAVAFKNSGLGMGVPDTGRCVLSVEQGMVHVRT"
BASE COUNT          331 a          705 c          746 g          281 t
ORIGIN      
        1 gacccgcggc ccacgtggac ggtgcgcgtc gcggacctgc acgagaatgt cgaccacacc
       61 ccgtacgagg ggacggaggt gaccggccgc gtccgggacg tcttcctgcg gggccaccgc
      121 gccgtcgtcg acggccacct cgccgacgag ctgccgcagg gggtcttcct cccctgtggc
      181 acgcccgata cgtcagtcgt ctgaacaatg gccacgagcc gaaaggacgc cacatgagca
      241 tcatggtgaa cgggcaggcc cacgacatcc ccggggcgat gaccctgatg cgttacctgc
      301 gcgaggacct cgccctgagc tccgtgaagc acggctgcga tgacggcagt tgcggttcgt
      361 gcctcgtggt cgtggacggg aagaccgtca aggcctgcac ccgggacgtc accacgttcg
      421 atggccggca tgtcacgacg gcggaggggc tcaccgagcg cgagcgggcg gcgatcgtgt
      481 ccgcctacga gcaggagggc gccgtgcagt gcggcttctg catccccggg ttcgtgatgg
      541 ccacgaaggc cctgctggac aagagtcccg aacccggccg cgacgaggtc acgaaggcca
      601 tccgctccaa cgtctgccgc tgcaccgggt atcggatgat cgaggatgcc gtcctgcgct
      661 cggggcggat cctgcggggc gaggaggaga tcccgccccc gccggagcac atccggatgt
      721 cagaccgggc gccccgcctc gacgcggccc cgaaggtcct cgggaccggc ctgtacgccg
      781 acgacctccg cctcgacggg atggtccacg cgaaggcgct gcggtcggcg cacccgcgcg
      841 ccgtcgtcga gaggatcgac gtcagccggg ccgccgcgca ccccgactgc ctcgccgtgc
      901 tgaccgcacg cgacgtcccc gagaacacga tcggccacct cgtgaacgac tgggacgtgt
      961 tcatcgccgc gggcgacacg acccgctacg tcggggacgc cctcgcgctc gttgtctgtg
     1021 agcacgagga gaccctggac gaggtgatcg gcctcatcga cgtcgcgtac accgtactcg
     1081 agcccgtcac cagcccggcg caggcactcg cgcccggcgc tcccctgatc catcccggcg
     1141 gcaacgtcct cacccacgag caggtcaagc gcggtgacgc ggacgcggcg ctggcggcct
     1201 cggcgttcgt ggtgacgaac acgttccaca ccccgtggca ggagcacgcc ttcctcgaac
     1261 ccgagtgcgc cgtggcgcag tcgtggggcg agggcggcgt ggaggtgttc acgggcggac
     1321 agtccgtcta cgacgagcgg cgggagatct cccgcatgct gggcttgccg gcggagaggg
     1381 tccactccca ctcgatgctg gtgggcggcg ggttcggtgg caaggaggac atgagcgtcc
     1441 agcaccacgc ggcactcgcg gcatggcaca cggggcggcc ggtcaaggtg aagttctccc
     1501 ggcaggcgag cctcgactac cacgtcaagc gccacccgat ggacctggag gtcaccctcg
     1561 gctgcgacga gacgggccag ctcacggcgg cgaaggtgcg gatcgtggcg gacacgggtg
     1621 cttacgcatc gctcggcggg ccggtgctgc agcgcgcggt gacgcacgcc ggcggaccgt
     1681 acaactacca gaacttcgat tgcgaaggat tggcggttta cacgaacaac ccggtgtccg
     1741 gggcgttccg ggggttcggc gtcgcgcaga gcgacttcgc gctggagaac agcgtgaacc
     1801 tgctcgccgc gaaggccggg atcgacccgt gggaattccg gtaccgcaac gccatccggc
     1861 ccgggcagga gatgccgaac gggcagatcg cggacgaggc gaccgggctc gtcgagtgcc
     1921 tcgaggcgct gaagccggac tgggacgcgg atcctgccgc aggactggcc gtggcgttca
     1981 agaactccgg gctcggcatg ggggtgccgg acacgggccg gtgcgtgctc tcggtggagc
     2041 agggcatggt gcatgtccgc acc
//
LOCUS       DASRKH010000016         2023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_8522, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000016 DASRKH010000000
VERSION     DASRKH010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8522"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..792
                     /locus_tag="VFC06_00270"
     CDS             91..792
                     /locus_tag="VFC06_00270"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HZL80371.1"
                     /translation="MRTSPRKIGLLGGISWHSTLDYERLLHEGVAAALPEHTADLVIR
                     HYDFVAIGRAQAAGDWDGLGRQLAADARWLVDGGAEAIVICANTMHLVAPAVEQAVDV
                     PVIHIVDETAKAIRTAGLDTVALLGTGFTMRMPFYRDRMAMHGVTTIVPEEPDLQAIH
                     DLIYTDLTRGVVTAEGRALATRVTADVLARGAQGVIAGCTEIPMVMTADDVDVPYFDA
                     LALHARAAVEFALAD"
     gene            920..>2023
                     /locus_tag="VFC06_00275"
     CDS             920..>2023
                     /locus_tag="VFC06_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80372.1"
                     /translation="MTLPERVVSPSQAVIPDWCQRDQMAASHLVAHDALRRGPRERAQ
                     MLRRIGIRKIVWDWRDEHLGAFDAELDALRQYEVELSGIWTPLPMPAYEEPDYATRFG
                     LVPTAIKTLITEAARRGFAPDLWTQIGFGQPGAPAQLSERVHRVEVNRAADHLAGLAR
                     LARGHGMCIVLTNHGGWAGEPRTLVDVVRELARRGLGNVGIGFRLQHAHHLIADLDHH
                     LSTMDEHLVALMLSGADAGAELTGRVILPFGAGSRDRWVTHAVLESGWRGQLVVHAVG
                     QDDSEARLLDSLDGWEWSVDRFLRRPRLRPTPRILEPTWPPKPDWTGRAVPPDRGGHA
                     LRADAALAHRTATAAMRDNPHSGPGAAAASPDTH"
BASE COUNT          324 a          722 c          668 g          309 t
ORIGIN      
        1 gggagcacgg gtcggccggg cagcgcaggt cggcacttcc ggtgccaccc ggctcctcca
       61 ggcacccggc gggcggccta acctggggca atgcgcactt cacctcgcaa gatcggcctg
      121 ctcggcggca tttcctggca ctcgacgctc gactacgagc gcctccttca cgagggcgtc
      181 gccgcagcgc tacccgaaca cacagcggac ttggtgatcc ggcactacga cttcgtcgcg
      241 atcggtcggg cgcaggccgc cggcgactgg gacggcctcg gccggcagct cgccgccgac
      301 gcgcggtggc tcgtggacgg cggagccgag gcgatcgtca tttgcgccaa caccatgcac
      361 ttggtggcgc ccgccgtcga gcaagcagtc gacgtccccg tgatccacat cgtcgacgag
      421 acggccaagg cgatccgaac cgcgggcctc gacaccgtcg cgctgctggg cacgggattc
      481 acgatgcgca tgccgttcta ccgcgaccgc atggccatgc acggcgtcac cacgatcgtg
      541 ccggaagagc cggacctgca ggcgatccac gacctgattt acacggacct cactcggggt
      601 gtcgtcacgg ccgagggacg cgcactggcg acccgcgtca ccgccgacgt gctcgcgcgc
      661 ggcgcccaag gcgtcatcgc cggctgcacc gaaattccga tggtcatgac ggccgacgac
      721 gtcgacgtgc cctacttcga cgctctcgcg ctccatgccc gggccgccgt ggaattcgct
      781 ctcgcggact gaaaaccctg ctaatcgcga catatcggcg cgtatatggg tgtttcgccc
      841 atatgggtct cgccctatat accactgcga aatcccggtc tatcgtaaag acacgcacac
      901 gaggtaaggg ggtctcgaag tgacattgcc agaacgtgtc gtttcgccgt cccaggccgt
      961 gattcctgac tggtgccagc gtgatcagat ggcggcaagc cacctggtcg ctcacgacgc
     1021 actacgtcgc ggcccccgtg agcgcgccca aatgctgcga cgcatcggca tccgcaagat
     1081 cgtgtgggac tggcgcgacg agcacctggg cgccttcgac gccgaactcg acgcgcttcg
     1141 ccaatacgag gtcgagctgt ctggcatctg gacgccgctt cccatgccgg cgtatgagga
     1201 gcccgactac gccacccgct tcggccttgt cccgaccgct atcaagacgc tcatcaccga
     1261 ggccgcccgc cgcggcttcg cgcccgacct ctggacccag atcgggttcg gccagccggg
     1321 cgccccggcg caactgtcgg agcgcgtgca tcgcgtggaa gtgaaccgtg cggccgacca
     1381 cctcgcgggg ttggcgcggc tggccagggg ccacggcatg tgcatcgtgc tcaccaatca
     1441 cggcgggtgg gccggcgagc cgcgcacgct ggtagacgtg gtgagggaac tggcgcgccg
     1501 cggcctaggc aacgtcggga ttggattcag gctccagcac gcccatcacc tcattgccga
     1561 cctcgaccac cacttgagca cgatggacga acacctggtg gcgctcatgc tcagcggcgc
     1621 cgatgccggg gccgagctca ctggccgggt catccttccc ttcggtgccg gcagcaggga
     1681 ccggtgggtg acgcatgccg tgctcgaatc gggctggcgg ggacaactcg tggtccacgc
     1741 ggtcggtcaa gacgactcgg aggcgcggct gctggacagc ctcgacggct gggaatggtc
     1801 ggtcgaccgc ttcctgaggc gcccgcgatt gcgtcccacg ccacggattc tcgagcccac
     1861 gtggccgccc aagcctgact ggaccggacg ggcggttcct ccggatcgcg gagggcacgc
     1921 cctgcgcgcc gacgctgctc tcgcgcatcg cacggcgacg gccgccatgc gggataaccc
     1981 gcattccgga ccgggcgcgg ccgccgccag tccggacacc cac
//
LOCUS       DASRKH010000017         2136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_35442, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000017 DASRKH010000000
VERSION     DASRKH010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35442"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /gene="rplF"
                     /locus_tag="VFC06_00280"
     CDS             complement(<1..478)
                     /gene="rplF"
                     /locus_tag="VFC06_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HZL80373.1"
                     /translation="MSRIGRIPVPVPAGVDVKIDGAVVTVQGPKGTLAHTVATPIEVA
                     RDDAGALVVTRPNDERASRSLHGLTRTLLANLVIGVTDGYEKKLEIVGTGYRVAAKDG
                     GLEFALGFSHPVAVAAPAGITFAVEGPTKFSVIGIDKQQVGEVAANIRKIRKPEPYK"
     gene            complement(503..901)
                     /gene="rpsH"
                     /locus_tag="VFC06_00285"
     CDS             complement(503..901)
                     /gene="rpsH"
                     /locus_tag="VFC06_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003974254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HZL80374.1"
                     /translation="MTMTDPIADLLTRLRNANSAHHDSVTIPYSKLKSHIAEMLQAEG
                     YIASWKVEDAPVGKYLIIELKYGPNRERSLAGIKRVSKPGLRVYAKSTNLPKVLGGLG
                     VAILSTSSGLLTDKQAAKKGVGGEVLAYVW"
     gene            complement(976..1161)
                     /locus_tag="VFC06_00290"
     CDS             complement(976..1161)
                     /locus_tag="VFC06_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HZL80375.1"
                     /translation="MAKTALIQKAAGTPKFKVRAYTRCQRCGRPHSVYRKFGLCRICV
                     REMAHRGELPGVTKSSW"
     gene            complement(1164..1748)
                     /gene="rplE"
                     /locus_tag="VFC06_00295"
     CDS             complement(1164..1748)
                     /gene="rplE"
                     /locus_tag="VFC06_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013601823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HZL80376.1"
                     /translation="MTAIDENTPAVEVRQPRLKTRYAEEIVSALHEEFKHANVNQVAR
                     LVKVVVNMGVGDAAKDSKLMDGAVRDLTLITGQKPQVTKARKSIAQFKLREGMPIGAH
                     VTLRGDRAWEFLDRLLSTALPRIRDFRGLSAKQFDGHGNYTFGLTEQAMFHEIDQDKI
                     DRVRGMDITVVTTATTDDEGRSLLKRLGFPFKED"
     gene            complement(1748..2089)
                     /gene="rplX"
                     /locus_tag="VFC06_00300"
     CDS             complement(1748..2089)
                     /gene="rplX"
                     /locus_tag="VFC06_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HZL80377.1"
                     /translation="MAKIKKGDLVVIITGRDRGKQGRVLEVLKDSDRLVVEGVQRVTK
                     HVRAGQTSRGTRTGGIETIEAPIHISNVMLVDPETKKGTRVGYRTEQVERDGRTRTVR
                     VRVAKRSGKDI"
BASE COUNT          324 a          698 c          706 g          408 t
ORIGIN      
        1 ccttgtaggg ctcaggcttg cggatcttgc gaatgttcgc ggcgacctcg ccgacctgct
       61 gcttgtcgat gccgatgacc gagaacttcg tcgggccctc gacagcgaac gtgatgccgg
      121 ccggggctgc gacagcgacc gggtggctga acccgagtgc gaactcgagc ccaccgtcct
      181 tcgccgcgac gcggtaaccg gtgccgacga tctcgagctt cttctcgtag ccgtcggtca
      241 cgccgatgac gagattcgcc agcagcgtgc gcgtcagccc gtgcagcgag cgcgaagcgc
      301 gctcgtcgtt cgggcgagtg accacgaggg cgcctgcgtc atcacgcgcg acctcgatag
      361 gtgtggcgac cgtgtgcgcc agggtgccct tgggcccctg gaccgtcacg acggcgccgt
      421 cgatcttgac atccacgccg gccgggaccg ggacggggat tctgccgata cgagacatgg
      481 ctagctcctt tccgtctcgg acttaccaga cgtaggcgag gacttcccca cccacgccct
      541 tcttggcggc ctgcttgtcg gtcaggagac cggaggacgt ggacaggatc gccacgccaa
      601 ggcctccgag caccttgggc aggttggtcg acttggcata cacacgcagg cccggcttgg
      661 acacgcgctt gatgccggcg agcgaacgct cgcggttggg gccgtacttg agctcgatga
      721 tgaggtactt gcccacaggg gcgtcctcca ccttccagct cgcgatgtag ccctcggcct
      781 gcagcatctc agcgatgtgc gacttcagct tcgagtacgg aatggttacc gagtcgtggt
      841 gcgccgagtt cgcattacgc agacgggtca ggaggtctgc gatcgggtcg gtcatcgtca
      901 tggggcttca gccctctctc atcggggtat ctgcaccgcg tctcgcgacg cggtgcagac
      961 ctacgacgta gttggttacc agctgctctt ggtcacgccg gggagctcgc cgcggtgcgc
     1021 catctcgcgc acgcagatac ggcagagccc gaacttgcgg tacacggagt gcggacgtcc
     1081 gcaccgctgg caccgcgtgt aggcgcggac cttgaacttc ggggtcccgg cagccttctg
     1141 gatcagggcg gtcttcgcca ttgtcagtcc tccttgaagg ggaagccgag acgcttcaga
     1201 agcgagcggc cctcatcatc tgtcgtcgcg gtcgtcacga cagtgatgtc catgccgcgc
     1261 actcggtcga tcttgtcctg gtcgatctca tggaacatcg cctgctccgt gaggccgaag
     1321 gtgtagttgc cgtgcccgtc gaactgcttg gccgagagcc cacggaagtc gcggatgcgc
     1381 ggcagcgcgg tcgacaggag acggtcgagg aactcccagg cacggtcgcc gcgcagcgtg
     1441 acgtgcgcgc cgatcggcat cccctcacgc agcttgaact gcgcgatgga cttgcgtgcc
     1501 ttggtgacct gcggcttctg gcccgtgatc agggtcaggt cgcggaccgc accgtccatg
     1561 agcttggagt ccttggcggc gtcaccgaca cccatgttca cgacgacctt cacgaggcgc
     1621 gcgacctggt tgacgttggc gtgcttgaac tcctcgtgca gcgccgagac gatctcctcg
     1681 gcgtagcgcg tcttcaggcg cggctggcgc acctcgaccg cgggggtgtt ctcgtcgata
     1741 gcggtcatca gatgtcctta ccggagcgct tggcgacgcg gacccggaca gtgcgggtac
     1801 ggccgtcgcg ctcgacctgc tcggtgcggt agccgacccg ggtgcccttc ttggtctccg
     1861 ggtccaccag catcacgttg ctgatgtgga tcggggcctc gatcgtctcg atgccaccgg
     1921 tccgggtgcc gcggctcgtc tggccggcgc ggacatgctt ggtcacccgc tgcacaccct
     1981 cgacgacaag acggtcggag tccttgagga cctccaggac gcgtccctgc ttgccgcggt
     2041 cgcgccccgt gatgatgacg acgaggtcgc ccttcttgat cttggccatg gtcagagcac
     2101 ctccggcgcc aacgagatga tcttcatgaa cttctt
//
LOCUS       DASRKH010000018         2242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_48634, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000018 DASRKH010000000
VERSION     DASRKH010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48634"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..1375)
                     /gene="tig"
                     /locus_tag="VFC06_00305"
     CDS             complement(47..1375)
                     /gene="tig"
                     /locus_tag="VFC06_00305"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HZL80378.1"
                     /translation="MTSALEKIDSTTVKITVTLTEEEVAPSMEHAYEHIGREVTIPGF
                     RKGKVPAKILEQRVGKGAVIEHAVNDGVPGWYAAAIQELSVRPYGQPDIEVTKLPGIV
                     EGDEGLEFVATVEVRPEITLPAPADLVIEVAPIEVSEEDIQERMTALQERFGTLVAVD
                     RAAEDGDFVTLDLNAEISGESVDAVQGTSYQVGTGTMIEGLDAAVTGLSAGESTTFSG
                     PLAAGDFAGEPAAIAVTVTAVKTRELPEANDDFAQLASEFDTVEELKADLSEQAARIK
                     TNNQAMEAREKLLQVLTEKVGEFDLPGKIIEAEVHGHLENEDRLEDEEHRVEVSARAK
                     EALRTQMLLDQLAEDLEIDVQENELVDYLVNASRQYGTDPSEFIQQVQQAGQIPAIVA
                     DVARSKATAYALRRATVKDSNGNPVDLKAIIGGLEDEVESTPEAEEAVAE"
     gene            complement(1497..>2242)
                     /locus_tag="VFC06_00310"
     CDS             complement(1497..>2242)
                     /locus_tag="VFC06_00310"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZL80379.1"
                     /translation="YPGDMTSSISAPVPLVEPATLEELSAVHSEILAPSFPAYELMSL
                     ERLTSEVEHGDTSVRVIRGDDGQLAGCAIGTWYEEARVLLLDYLALRPGARGGGLGGT
                     LLNASVDAWSNEYDPCIVLAEVEHPDHHDAHPQHGDPEARLRFYAREGARLLPLPYFT
                     PGVGDGKPRTGGMMLAVLHAHPDLLGENDEEIASEPIATVMSLKAGDEADYDEAQTEL
                     IAAAEAHPTLPLLRADRVGDVPIGIPDED"
BASE COUNT          344 a          744 c          729 g          425 t
ORIGIN      
        1 aaagcccggg tccctcagag ggacccgggc tttcgtgtct cgtgaactac tcggcgacgg
       61 cttcttccgc ctcgggggtc gactccacct cgtcctcgag gccaccgatg atggccttca
      121 ggtcgacagg gttgccgttg ctgtccttga cggtcgcccg acgcaaggcg taggcggtcg
      181 ccttcgagcg ggcaacgtcg gcgacgatcg cggggatctg accagcctgc tggacctgct
      241 ggatgaactc gctcgggtcc gtgccgtact gacgtgacgc attcaccagg tagtcgacaa
      301 gctcgttctc ctggacgtcg atctcaaggt cctcggcgag ctggtcaagc agcatctggg
      361 tacgcagggc ctccttggcg cgcgcgctca cctcgacgcg gtgctcctcg tcctcgagtc
      421 ggtcttcgtt ctcaaggtgg ccgtgcacct cggcctcgat gatcttgccg ggcaggtcga
      481 actcgccgac cttctcggtg aggacctgga gcagcttctc gcgtgcctcc atggcctggt
      541 tgttggtctt gatgcgtgcg gcctgctcgc tcaggtcggc cttgagctcc tcgacggtgt
      601 cgaactctga ggccagctga gcgaagtcgt cgttcgcttc gggaagttcg cgggtcttca
      661 cggccgtgac ggtgaccgca atcgcggcgg gctcgcccgc gaagtcaccg gcggccaatg
      721 ggcccgagaa cgtggtcgac tcgcctgccg acaggccggt cacggcggcg tcgaggccct
      781 cgatcatcgt gccggtaccc acctggtagg acgtgccctg gactgcgtcc actgactcgc
      841 cgctgatctc cgcgttgagg tccagcgtca cgaagtcgcc gtcctcagcc gcacggtcca
      901 cggcgaccag ggtgccgaat cgctcctgca gcgcggtcat gcgctcctgg atgtcctctt
      961 cgctgacctc gatcggggca acctcgatga ccaggtccgc gggcgcgggc agcgtgatct
     1021 cggggcgcac ctcgacggtt gcgacaaact cgaggccctc atcgccttcg acgatgccgg
     1081 gaagcttggt gacctcgatg tcgggctgtc cgtacggacg caccgacagc tcttggatgg
     1141 ccgcggcata ccaacctggc acgccgtcgt tcacggcgtg ctcaatgact gcacccttgc
     1201 cgacgcgctg ctcaaggatc ttcgcgggca ccttgccctt gcggaatccg gggatcgtca
     1261 cctcgcggcc gatgtgctcg taggcgtgct ccatggacgg agcgacttcc tcctccgtca
     1321 gcgtgaccgt gatcttcacg gtggtggagt cgatcttctc gagggcgctg gtcacagact
     1381 tctcctgatg tgtcgatggg ggcccgagcg tgtacgggtc attcctgctt tcgggcaacc
     1441 caccaatcgt actggctcag gcgcgtcgac cccaatcgaa cggacccagc cgcccgtcag
     1501 tcttcgtcgg gaatgccgat tggcacgtcg cccacacggt cggcgcgcaa tagcgggagc
     1561 gtcgggtgag cctctgcggc agcgatgagt tcggtctgtg cctcgtcgta gtctgcctcg
     1621 tcaccggcct tcagagacat caccgtggcg atcggctcgg acgcgatctc ttcgtcgttt
     1681 tcgcccaaca agtccgggtg ggcatgcagg actgccagca tcatgccgcc cgtgcgcggc
     1741 ttcccgtcgc ccacgcccgg cgtgaagtac ggcaacggca acaagcgagc gccctcgcgg
     1801 gcatagaagc gcagccgcgc ctcggggtcg ccatgctgcg ggtgagcgtc atggtggtcc
     1861 gggtgctcga cctcggcaag gacgatgcac gggtcgtatt cgttcgacca ggcatcgacg
     1921 ctcgcgttga gcaacgtgcc gccaaggccg ccgccgcgcg cgccaggccg gagcgcgagg
     1981 tagtccagca gcaagacgcg cgcctcctcg taccaggtgc cgatcgcgca acctgcaagt
     2041 tggccgtcat cgccccgaat gacgcgcacc gacgtgtcgc cgtgctccac ctcggaggtc
     2101 agacgctcca aactcatcaa ttcgtatgcg ggaaacgatg gggcaagaat ctcggaatgg
     2161 actgccgaga gctcctcgag cgttgcgggt tcaaccaggg gcacaggcgc agatattgag
     2221 gaggtcatgt ctccaggata cg
//
LOCUS       DASRKH010000019         2524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_35308, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000019 DASRKH010000000
VERSION     DASRKH010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35308"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VFC06_00315"
     CDS             <1..470
                     /locus_tag="VFC06_00315"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HZL80380.1"
                     /translation="DAVLLLARAKAEEVAASGTSSLVLGCDSMLEFDGEVMGKPGSAE
                     IARQRWRAMRGGTGVLHTGHWLVDARAGAGSHTGQTASTTVHFADLTDSEIDAYVATG
                     EPLNVAGAFTVDGIGGPFVERIEGDHHNVVGVSLPVLRHLLGECGVRIQDLWS"
     gene            652..2442
                     /locus_tag="VFC06_00320"
     CDS             652..2442
                     /locus_tag="VFC06_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HZL80381.1"
                     /translation="MPAFTSVLIANRGEIAVRVIRACQDASLRSIAVYAESDRDAPHV
                     ALADDAYSLGGTTARESYLDIAKILDVARRSGAEAIHPGYGFLSENAEFAQAVLDAGL
                     VWIGPPPAAITALGDKVSARHIAQAAGAPLVPGTPGPVESADEVIAFADEFGLPVAIK
                     AAFGGGGRGLKVARNREDIPEMFDSATREAVAAFGRGECFVERFLDKPRHVETQCLAD
                     THGNIVVVSTRDCSLQRRHQKLVEEAPAPFLTDDQNALLVQSSQAILREAGYVGAGTC
                     EFLIGVDGTISFLEVNTRLQVEHPVTEEVTGVDLVREQFRIAASEPIANLNPQVRGHS
                     IEFRINGEDPAGGFMPAPGRINILRLPAGPGVRVDAGVREGDTVSGNFDSMIAKLIVT
                     GATRAEAIQRARRAVSEFVVRGIPTVLPFHAAVLDAPEFVGEDGYGVYTTWIETGFAE
                     TVKSLGPSGEAFSGHDDGAATERVVVEVGGKRLEVVLPAALAQAGRARARAGAPARRT
                     TKKASTVRVSSSVTLSSPMQGTIVKVAVEDGATVAEGDLVVVLEAMKMEQPLKAHRAG
                     VVSSLTALVGSSVTAGELICDIIDAPSPSE"
BASE COUNT          394 a          841 c          862 g          427 t
ORIGIN      
        1 ccgatgcagt attgcttctg gcccgcgcca aggcggagga ggtcgccgcg agcggaacca
       61 gctcgttggt gctcggctgc gattcgatgc ttgagttcga tggtgaggtg atggggaaac
      121 ctggaagtgc cgagatcgcc cgccaacggt ggcgggccat gcggggcggg acgggtgtgc
      181 tccacaccgg gcattggctc gtcgacgcgc gtgcaggcgc gggctcgcac acgggccaga
      241 cggcgtccac cacggtccac ttcgccgacc tgacggacag tgagatcgac gcctacgtcg
      301 ctaccggcga gccactcaac gtcgctgggg cgttcaccgt tgacgggatc ggcggcccct
      361 tcgtggagcg catcgagggc gaccatcaca acgtcgttgg cgtcagcttg ccggtcctgc
      421 gccatcttct gggcgagtgc ggagtgcgca tccaggacct gtggagttag gggatcctga
      481 gcggcagttt gtggcgtacc cacaacctag ggggaagcag cctccattct tggtcgattc
      541 gttgtaggat tcccacaaca tgggacccaa gcgctgtgct tccgaccaaa tccgcattcc
      601 agagggttcc gggtcggtac tagcggctgg ggccacggta ggttgagcga cgtgcctgcg
      661 ttcacctcgg ttctcatcgc caaccgtgga gagatcgccg tccgcgtgat ccgcgcctgt
      721 caagatgcct ccctgcgttc catcgctgtg tacgccgaat ccgaccgcga cgccccccac
      781 gtcgccctcg ccgacgacgc ctactccctc ggcggcacaa ccgcacgaga gtcctacctc
      841 gacatcgcga agatcctcga tgtcgcgcgt cgatcggggg cagaggcgat ccaccccggc
      901 tacggcttcc tgtcagagaa cgccgagttc gcgcaagcgg tgctcgacgc tgggttggta
      961 tggattggcc cgccgcccgc cgcgatcacg gcgctcggcg acaaggtgag cgcgcgtcac
     1021 attgcccagg cagcgggagc gccgctcgtt cccggcaccc cgggcccggt tgagtcggcc
     1081 gacgaggtga tcgcgttcgc ggacgaattt ggcctgccgg tcgcgatcaa ggcagcgttc
     1141 ggcggaggcg gtcgaggcct gaaggtggcc cgcaaccgcg aagatatccc cgaaatgttc
     1201 gactcggcca ctcgcgaggc agtcgcggcc tttgggcgcg gcgagtgctt tgtggagcgt
     1261 ttcctcgaca agccgcggca cgtggagacc cagtgcctgg cggacaccca cggcaacatc
     1321 gtggtcgtgt cgacgcgcga ctgctcgttg cagcggcgcc accagaagct ggtcgaggag
     1381 gcgcccgcgc cgttcctgac ggacgatcag aatgccctgc tcgtgcagtc cagccaggcc
     1441 atcctgcgtg aggctggcta cgtcggcgcg ggcacgtgcg agttcctcat tggtgtggac
     1501 ggcaccatca gcttccttga ggtcaacacc cgcctccagg tcgagcaccc cgtgacggaa
     1561 gaggtcacgg gcgtggacct ggtgcgcgag cagttccgca ttgcggcgag cgagccgatc
     1621 gccaacctca atccccaggt gcgcggccac tcgatcgagt tccgcatcaa tggcgaggac
     1681 cccgcagggg gcttcatgcc cgcgcccggt cgcatcaaca tcttgcgcct gcccgcgggc
     1741 cccggcgtcc gcgtcgatgc tggcgtccgc gagggcgaca ccgtctccgg caacttcgac
     1801 tccatgattg ccaagctcat cgtcacgggc gccactcgcg ccgaggcgat tcagcgcgcg
     1861 cgtcgggccg tctccgagtt cgtggtgagg ggcatcccca cggtgctgcc cttccacgcg
     1921 gcggtgctcg acgcccccga gtttgtgggc gaggacggct acggggtcta caccacgtgg
     1981 atcgagaccg gctttgccga gaccgtgaag tcgctgggcc cgtcaggtga agctttctcc
     2041 ggtcacgacg acggcgcggc caccgagcgc gtcgtcgtcg aggtgggcgg caagcggctg
     2101 gaagtcgtcc ttccggcggc cctcgcgcag gcaggccggg cgcgtgcccg tgcgggtgcg
     2161 cctgcacgtc gcaccaccaa gaaggcgtcg acggtgcggg tctcgtccag cgtcaccctg
     2221 tcctcgccga tgcaggggac catcgtcaag gtcgcggtcg aggacggcgc cacagtggcg
     2281 gagggcgacc tcgtggtggt gcttgaggct atgaagatgg aacagccgct caaggcgcac
     2341 agggcgggcg tggtgagcag cctgacggca ctcgtgggct cttcggtgac cgctggcgag
     2401 ttgatctgcg acatcatcga cgccccctcc ccctcggaat aggcgctccg ggctctttcg
     2461 ccccacccca gcgaattacg caacgctttc gttgcgaaat gttgacacag gccgccaatg
     2521 cccc
//
LOCUS       DASRKH010000020         3116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_21825, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000020 DASRKH010000000
VERSION     DASRKH010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21825"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /gene="carB"
                     /locus_tag="VFC06_00325"
     CDS             <1..377
                     /gene="carB"
                     /locus_tag="VFC06_00325"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015771605.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HZL80382.1"
                     /translation="RLYQLGFTLLATEGTRRVLLRHGIPSHVVRKNSQGKGPNGEPTI
                     VDLINDGQVDMVVNTPSGQGARADGYEIRAAATAGDVAIITTTQQLAAAVQAIEALQR
                     GRFEVMSLQDVARSIAMQRTPR"
     gene            429..1286
                     /gene="pyrF"
                     /locus_tag="VFC06_00330"
     CDS             429..1286
                     /gene="pyrF"
                     /locus_tag="VFC06_00330"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HZL80383.1"
                     /translation="MTFGARLDASIQQWGPLCVGIDPHPELLARWGLTDTAASLMAFS
                     DAVLEASVRSVAAVKPNAAFYERHGARGVAALEHTLKSARDMGLMTILDVKRGDIGST
                     MEGYADAYLAEGVPLEADAITVSPYLGFGSLRPAFELAAHTGKGVFVLALTSNPEGAS
                     VQHATDANGISVARAIAEGAAELNAGATPMGSIGLVVGATVGDAVRELGIDLAAVNGP
                     LLSPGVGAQGAGPAEVAQVFGSARSRVLVSQSRGILMAGPFVEKLRGAIQAAAGAAQY
                     ALGTAEAGE"
     gene            1324..1638
                     /gene="mihF"
                     /locus_tag="VFC06_00335"
     CDS             1324..1638
                     /gene="mihF"
                     /locus_tag="VFC06_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013601071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HZL80384.1"
                     /translation="MATPVLSDEQRAQALVKATAVRSARRVFKESLSQGHMTLSEAIV
                     RAKADDALAGIRVHDLLQCLPGIGPKRAALVMEDIGISPARRVRGLGSHQVDALVARE
                     RG"
     gene            1635..2189
                     /gene="gmk"
                     /locus_tag="VFC06_00340"
     CDS             1635..2189
                     /gene="gmk"
                     /locus_tag="VFC06_00340"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HZL80385.1"
                     /translation="MSRLVVVAGPSAVGKGTLVAALKERMPEVWVSVSATTRPARPTE
                     REGVHYFFVSPEEFDRMTERGQLLEWAVVHGTHRYGTPRGPVEDRLARGVPVVLEIDL
                     QGARQVKQAMPDALFVFVAPPSFETLVERLEIRGTEGSEERERRLRTATEELAAASEF
                     DITLVNDRLEDAVAELQTIVASAR"
     gene            2242..2508
                     /gene="rpoZ"
                     /locus_tag="VFC06_00345"
     CDS             2242..2508
                     /gene="rpoZ"
                     /locus_tag="VFC06_00345"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HZL80386.1"
                     /translation="MSGTVAHPEGITNPPIDELLAKVDSKYALVIYGSKRARQINAYY
                     EALGEGHFENVGPLVEADSTDKPLSVALREINAGNLLVLGETEV"
     gene            2515..>3116
                     /locus_tag="VFC06_00350"
     CDS             2515..>3116
                     /locus_tag="VFC06_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HZL80387.1"
                     /translation="MRVLLGVTGGIAAYKVALVVRRLMEDGHSVRVIPTESSLNFVGR
                     ATWEALTGEPARSGTFENVPSVEHVTLGQRADIVLVAPATANFLASMAAGEARDLLGN
                     ALLATKAPVVVAPAMHTEMWNNPATVANIATLRSRGVHVIEPTVGRLTGADSGPGRLP
                     EPDDLVAALYAVADRSPIDVGASPGAKHEWGDLGGVRIVIS"
BASE COUNT          531 a          988 c         1065 g          532 t
ORIGIN      
        1 cacggctcta tcagctggga ttcaccctct tggcaaccga gggtacgcgc cgagtgctgt
       61 tgcgccacgg aattccctcc cacgtggtcc gcaagaattc tcagggaaag ggccccaacg
      121 gggagcccac cattgtggac ctcatcaacg acggtcaggt ggacatggtc gtcaacacgc
      181 cgtccggcca aggggcgcgc gcagacgggt acgagatccg tgcggccgca acggccggag
      241 acgtcgcgat catcaccacc actcaacagc tcgcggcggc ggtgcaggca attgaggcgc
      301 tccagagagg gcgctttgag gtgatgagtc tgcaagatgt ggcgcgctcc atcgccatgc
      361 agcgaacgcc caggtagagt ggcttccata acatgaccgg ggttgtccag gcaaggaacg
      421 gaggtcccat gacattcggc gcgcgactcg acgcttccat ccagcaatgg ggccccctgt
      481 gcgtcggcat cgacccccac cctgaactgc tcgctcggtg gggtcttacg gacaccgcgg
      541 cgtcattgat ggcgttctcg gatgccgtgc tggaggcctc cgtgaggtcc gtcgcagcgg
      601 tcaagcccaa cgcggcgttc tacgagcggc acggagccag gggagtggct gcactggagc
      661 acacgctcaa gagcgccagg gatatgggcc tgatgacgat tctggacgtg aagcgcggag
      721 acatcgggtc gaccatggag gggtatgccg acgcgtatct cgccgagggc gtgccgcttg
      781 aggccgacgc gatcactgtc tccccgtacc tgggctttgg atccttgcgg ccggcgttcg
      841 agctggccgc acacaccggc aaaggagtgt tcgtgcttgc gctgacctcc aacccggaag
      901 gcgcgtctgt ccaacacgcg accgacgcga acggcatctc cgtggcgcgc gcgattgctg
      961 aaggagccgc cgaattgaac gctggcgcta cccccatggg ctcgattggg cttgttgtag
     1021 gggcaaccgt cggggatgcc gtgcgtgagc tcggtatcga tcttgccgcc gtcaacggac
     1081 cccttctctc cccaggtgta ggcgcccaag gcgccggtcc ggctgaagtc gcgcaagtat
     1141 ttggatcagc ccgttcccgc gtcctcgtga gtcaatcgcg ggggatcttg atggctggac
     1201 catttgtaga gaagctgaga ggcgccattc aggccgccgc cggagcagcc cagtacgctt
     1261 tgggcacggc ggaggcaggc gagtaacgcc gcgagtccca cggcgtttca acaaggaggt
     1321 ggcatggcta caccggtact tagtgacgag cagcgcgctc aggcgctcgt caaggcaacg
     1381 gcggtgcgca gcgcgcgtcg cgtcttcaag gagagtctgt ctcaaggtca tatgacgctc
     1441 agtgaagcta tcgtgcgtgc aaaggcggat gacgccttgg cgggcatccg ggtgcacgac
     1501 ttgctgcagt gcctgcccgg cattggtccc aagcgagcag ccctcgtgat ggaggacatc
     1561 ggcatctccc cggctcgccg cgtccgcggg cttggaagtc accaagtcga cgcactggtg
     1621 gcgcgcgaac gcggatgagc cgtctcgtcg tcgtggccgg cccctcggcc gtcggcaagg
     1681 gcaccttggt ggcagcactc aaggagcgca tgccggaggt gtgggtgtcg gtctcggcga
     1741 cgacgcggcc cgcccggccg accgagcggg agggagttca ctacttcttt gtgtcccccg
     1801 aggagttcga ccgcatgacc gagcgcggtc agctgctcga gtgggccgtt gttcacggca
     1861 cccaccggta cggcacgccc cggggcccgg tagaggatcg attggcacgc ggcgtccccg
     1921 tggtgctgga gattgacctt cagggcgcga ggcaggtcaa gcaggcgatg cccgacgcgc
     1981 tcttcgtgtt cgtcgcaccg ccatctttcg agacgctcgt cgagcgtctc gaaatccgcg
     2041 gcaccgaggg cagcgaggaa cgcgagcggc ggttgcggac cgcaacggaa gaactggccg
     2101 cggcgagtga gttcgatatc acgctcgtga atgaccgttt ggaggacgcc gtggccgagt
     2161 tgcagacgat tgtggcgtcg gcacgctaag ataggtggcg ttgtctgcct caaggtggga
     2221 gcgaacgacg agaggatttt cgtgtctgga accgttgcac atcccgaggg cattaccaac
     2281 ccgcccatcg acgagctgct tgcaaaggtc gactccaagt acgcgctcgt gatctacggc
     2341 tccaagcgtg cccgccagat caacgcgtac tacgaggcgc ttggcgaggg ccacttcgag
     2401 aacgtgggcc cgctggtcga ggctgactcg accgacaagc cgctctctgt cgcgctgcgc
     2461 gagatcaacg ctggcaacct tctcgtgctc ggcgagaccg aggtctagcc gcgcatgcgg
     2521 gtgctgctgg gcgttaccgg cggcatcgcg gcatacaagg tcgccttggt ggtacgccgc
     2581 ctcatggagg acgggcactc ggtgcgggtc atccccacgg agtcgtccct caatttcgtg
     2641 ggacgcgcca cgtgggaggc gctcacgggc gaacccgccc ggtccggaac gtttgagaac
     2701 gtgccgtccg tcgagcacgt gacgctcggc caacgcgccg acatcgtcct tgtcgctccc
     2761 gccaccgcca acttcttggc gtcgatggcc gccggggagg cccgggacct gctcggcaat
     2821 gcgctacttg cgaccaaggc gcccgtggtg gtcgcccctg ccatgcacac ggaaatgtgg
     2881 aacaaccctg cgactgtggc gaacatcgcg acgctgcggt cacgcggcgt tcacgtgatc
     2941 gagcccaccg tcggccgcct gaccggcgcc gattccggtc cgggacggct tcccgagccg
     3001 gacgacctgg tggccgcgct gtacgcggtg gccgaccgct cacctatcga tgtcggcgca
     3061 tcgccgggcg ccaagcatga gtggggcgat ctcggcggag tgcgcatcgt catctc
//
LOCUS       DASRKH010000021         3147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_17409, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000021 DASRKH010000000
VERSION     DASRKH010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17409"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VFC06_00355"
     CDS             complement(<1..284)
                     /locus_tag="VFC06_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80388.1"
                     /translation="MTSEEELLAQIPEDARGEDFYSASQFAKFFIREYTRLFLEDASS
                     DLYAELSSDECAYCASALENARETAEVGAHSVGGAFSLPDVVGRGGLRDDG"
     gene            complement(587..2047)
                     /locus_tag="VFC06_00360"
     CDS             complement(587..2047)
                     /locus_tag="VFC06_00360"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZL80389.1"
                     /translation="MTSSFAELGVPTQLTDLLDREGKSKAFPIQEDTLPDTLAGRDVL
                     GRGKTGSGKTLAFALPMVARLGRSGGKSRPGHPRALVLAPTRELATQIDAVIAPLAKA
                     YGLTTTTIFGGVSQQRQERALRAGVDIVVACPGRLEDLMKQRHVTLDSVEITVLDEAD
                     HMADLGFLPGVTRIMTATRERGQRLLFSATLDNGVDKLVRRFLDKPVTHSVDSEDSHV
                     DAMTHHVFHVSGADAKRELVQALASGTGRRILFMRTKHQAKKLAKQLTAAGIPSVDLH
                     GNLSQGQRDRNLALFQGTTHRVLVATDVAARGVHVDDVELVVHVDPPAEHKSYLHRSG
                     RTARAGSAGDVVTVVLPEQRRETDQLLRRANIQVRPQAVSASSAQVATLVGDVAAFVK
                     PSPVAEQPQRQGQGQGRGRRSAPRAGARQGQSSGRPSQERHARPEGEHGQHRPAETRY
                     GQGRSAQGRSSSGGQRSNQGGAAAFSAGGSSGRRSR"
     gene            complement(2380..2622)
                     /locus_tag="VFC06_00365"
     CDS             complement(2380..2622)
                     /locus_tag="VFC06_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HZL80390.1"
                     /translation="MNIKVLGSGCANCVRLEANVKEALAATGIDDAVVEKVTDYADIA
                     AYGIMSTPGLVVNERVLVSGRVPAADEVATLLAAAR"
     gene            complement(2649..>3147)
                     /locus_tag="VFC06_00370"
     CDS             complement(2649..>3147)
                     /locus_tag="VFC06_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HZL80391.1"
                     /translation="SQTARPNLLASGGGSDAGGLAVETKPSAQLRVRIGLDEVREILG
                     KVWPYLLIGVGLGAVIHGWVPSDFFAAHAGPDNPFGVLVAVVLGVPLYSNAAGALPLV
                     EALADKGVPMGTLLAFMMSVVALSLPEMVLLKRVLKLPLLGAFAGVVAIGIVAVGYLF
                     NFILT"
BASE COUNT          533 a         1014 c         1113 g          487 t
ORIGIN      
        1 ccgtcgtccc gcaatccgcc ccttcccaca acgtctggca acgagaacgc cccgccgacg
       61 ctgtgagctc ccacctctgc cgtttcgcgc gcattctcca gggcgctggc gcagtaagcg
      121 cactcatcgc tgcttagctc cgcatagagg tcggaacttg cgtcctcaag aaagagtcgc
      181 gtgtactcac ggatgaagaa cttcgcgaac tggctagcag agtagaagtc ctccccccgc
      241 gcatcctcgg ggatctgcgc gaggagctcc tcttccgaag tcacagtcgg cgtcggactg
      301 ggcgacgggg tcgcttctac cgtccgggta agtgacggtg attccgtcac gatcggccca
      361 ggatcacctg agcatgcagt caacgatgcg gtcgcgaccc ccacgagggc cgcgaccgcc
      421 acacaacgca gcagtagagc catgcgcgag accataccgg gcaatcaaga cacctagtag
      481 ccccaatatt gggggctgtg gataacgtcg gtggatttct cttgccccga caaacacgaa
      541 gccgcgccga tcacgaagga tcggcgcggc ttcgagagtc gtactgctag cgcgaacgcc
      601 gacctgagga gccgccagcg gagaaggccg cggcgccgcc ttggttgctg cgctgcccgc
      661 cggaagacga acgaccctgc gcggatcgac cctggccgta gcgcgtctcg gccggacggt
      721 gctgaccgtg ctcgccctcg ggacgcgcgt ggcgttcctg ggagggacgc ccactggact
      781 ggccctgacg ggcaccggcc cgcggggcac tgcgtcgtcc cctgccttgg ccctgaccct
      841 ggcgctgcgg ctgctccgca acgggcgagg gcttgacgaa cgcggcaacg tcgccgacga
      901 gcgtcgcgac ctgtgcggac gaggcgctca cggcctgcgg acgcacctgg atgttggcgc
      961 ggcgcagcag ttgatccgtc tcgcgacgct gctcgggaag cacgacggtg acgacgtcgc
     1021 ccgcggagcc cgcacgcgcg gtgcggccgg agcggtgcag gtacgacttg tgctcggccg
     1081 gcgggtcgac gtgaaccacg agctcgacat cgtcgacgtg gacgccacgc gcggcgacgt
     1141 cggtggccac cagcacgcgg tgagtcgttc cctggaagag cgcgaggttg cggtcgcgct
     1201 gaccctgcga caggttgccg tgcagatcga cggacgggat tcccgccgcg gtgagctgct
     1261 tggcaagctt cttggcctgg tgcttggtgc gcatgaacag gatgcggcgg ccggtgcccg
     1321 acgcgagcgc ctggaccagt tcgcgcttcg catcggctcc gctcacgtgg aacacgtggt
     1381 gggtcatggc gtcgacgtgg ctgtcctcgc tgtcgaccga gtgagtgacg ggcttgtcga
     1441 ggaagcggcg caccaacttg tcgacgccgt tgtccagcgt cgcggagaac agcagtcgct
     1501 gtccacgctc gcgtgtagcg gtcatgatgc gggtcacgcc gggcaggaaa ccgaggtcgg
     1561 ccatgtggtc ggcctcgtcc agcacggtga tctcgacgct gtcgagcgtc acgtgacgtt
     1621 gcttcatgag gtcttcgagg cggccggggc acgccacgac gatgtcgacg ccggcgcgca
     1681 gtgcacgctc ctggcgctgc tggctcactc cgccgaagat cgtggtggtg gtgaggccgt
     1741 aggccttggc gagcggcgcg atgaccgcgt cgatctgggt ggcgagctcg cgggtcggcg
     1801 ccaggacgag cgccctgggg tgaccggggc gcgacttgcc gcccgagcgg ccaaggcggg
     1861 cgaccatggg caaggcgaag gcgagcgtct ttccagagcc cgtcttgccg cggccgagta
     1921 cgtcacggcc cgcgagggtg tcgggaagcg tgtcctcctg aatggggaac gctttggact
     1981 taccttcgcg atcgagcagg tcggtgagtt gagtggggac gccgagttcg gcgaaagagc
     2041 tggtcaaggg gatagtgcct ttcaggcatg gtgccggacg cgcatgagcg catcaccgtg
     2101 actgtcacgg caccggcatt ggtggcgaat cgcggaggaa tccgcgctcg ttcgccggag
     2161 agaagttcta cgagccgcgc ggccacaagg gcatgtgcgc tggcgagaag gagaagcaat
     2221 ctgcgacgca ggagggcttg ggaggccctg caagtactgc aaaggctatc agacccgcac
     2281 ttgcaggccc ttcgcggcgt gccggacggg tcggagtggg cccaggcccg ggcatgctga
     2341 caccggggcc cgcacgccag tcgctccccc gaaaaccccc tagcgcgcgg cggcgaggag
     2401 cgtcgcgacc tcgtcggcgg cgggcacccg gccactgacc agcacgcgct cattcacgac
     2461 gagccccggc gtcgacatga tgccgtacgc cgcgatgtcc gcgtagtccg tgaccttctc
     2521 gaccacagca tcgtcgatgc cagtagcggc aagggcttcc ttgacgttcg cttcgagccg
     2581 cacgcagttg gcgcaaccgg agccgaggac cttgatgttc atggtgttct ccttgagtga
     2641 gagtgacgtt aggtgaggat gaagttgaag aggtagccga cggccacgat gccgattgcc
     2701 accacaccgg cgaaggcccc caggagtggc agcttgagga cgcgcttgag gagcaccatc
     2761 tcgggcagtg acagggcgac gacgctcatc atgaaggcga gcagggtgcc catggggacc
     2821 cccttgtcgg caagcgcctc gaccaagggc agcgccccgg cggcgttcga gtagagcggc
     2881 accccaagga cgacggccac gagcactccg aacgggttgt ccgggcccgc gtgcgcggca
     2941 aagaagtcgg agggcaccca gccgtggatc acggcaccca agcccactcc gatgagcagg
     3001 tagggccaca ccttgccgag gatctcgcgc acttcgtcga ggccgatgcg tacgcgcagt
     3061 tgagccgacg gcttggtttc gacggccaag ccgccagcgt cggagccgcc acccgaggcc
     3121 aacaggttgg ggcgcgcggt ctgcgag
//
LOCUS       DASRKH010000022         2712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_44026, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000022 DASRKH010000000
VERSION     DASRKH010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44026"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1710)
                     /locus_tag="VFC06_00375"
     CDS             complement(<1..1710)
                     /locus_tag="VFC06_00375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02680 family protein"
                     /protein_id="HZL80392.1"
                     /translation="MRQDSPAPRPTTRRWQPLRLGLVDLFYYDDEQFWFHDGRLLLRG
                     NNGTGKSKVLALTLPFLLDGSLSAQRVEPDADPKKRMEWNLLLGGDHPNSERLGYTWV
                     EFGRVDDDGTEHFTTLGCGLKAVTGRGIARHWFFVTDRRIGDLRLVDANGVALARERL
                     RDELQGHGRVYDTREEYRHAVDEALFGLGESRYSALVDLLIQLRQPQLSKRPDARALS
                     GALTEALAPLDQAVIADVAESFRSLEEDRSRLLEAESTHDAAAAFLRHYAEYARVASR
                     RAAQGVRTAQSEYERVGRDQGVADEELARAQAELERVTDAEAQLALDHSSLLGTQDAL
                     RTSPEMDAAHELEHAATTAEQAQAAARLALGDVARSEEHRRATAREQETATATERDAR
                     ARFAQLGTEASGRSSAAGLGETHEALLADDGAARAALSRRREQLAHVGCLVAAADQAH
                     GVAEHQRQAFDNAQARTAQRDEQATASDRGVDEAVTGYAAELRDYVRSLTVLVVEDDD
                     DVLDLSAAWAVAQDGPAPARRAVERAAAAAASQIAANQTRAQHEESGLTVELAEVRER
                     LAEL"
     gene            complement(1707..>2712)
                     /locus_tag="VFC06_00380"
     CDS             complement(1707..>2712)
                     /locus_tag="VFC06_00380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR02678 family protein"
                     /protein_id="HZL80393.1"
                     /translation="PEPQQQADRGRQVRPLITTRERELDPLELRLPGQEHDVLGRLAE
                     NVVLLARQTELEGIEFALAGRDERSDLAAAIRLLLGLGVLARVAGDEESYVQSGGDAL
                     YDVDRRVLSRLLVVRHGPSVVVNTPGFPSGDLAIDELESALFAEPPAFTEDETNRRLR
                     HSLTRRLLEDPVVYYDELDEAERVYLTRQRAAITRRITEFTGLTAEIRAEGVAMVDPL
                     DQLTDVRMPESGTDGHATLLVAEHLAGAGPTEIDELRHLVRKLAGEYSTYWRRTAQQP
                     GAENAIVADAVRRLVDLGLVSVAGTTVTPRPALHRFSIGAPTIRAARDAPETSRGTDQ
                     "
BASE COUNT          342 a          972 c          984 g          414 t
ORIGIN      
        1 gagctcggcc agtcgctcac gcacctcggc gagctcaacg gtcagaccag actcctcgtg
       61 ctgggcccgc gtctggttcg ccgcgatctg gctggcggca gccgcagcgg ctcgctcgac
      121 cgcacgacgc gccggcgccg gaccgtcctg cgcgaccgcc cacgccgcgc tgaggtcgag
      181 cacgtcgtcg tcgtcctcga ccaccaggac ggtcaacgac cgaacgtagt cgcggagctc
      241 cgctgcgtac ccggtcaccg cctcatcgac gcctcggtca ctcgccgtgg cctgctcgtc
      301 gcgctgggct gtcctggcct gcgcgttgtc gaacgcctgc cgctgatgct cggccacccc
      361 gtgggcctgg tcggcggccg ccacgaggca cccgacgtgg gcgagctgct cgcggcgccg
      421 ggacagcgcg gcccgggccg cgccgtcgtc ggcgagcagg gcctcgtgcg tctcgccgag
      481 cccggccgcg gaggatcgac cggaggcctc ggtgcccagt tgcgcgaagc gcgcccgcgc
      541 gtcgcgctcg gttgcggtcg ccgtctcctg ctcgcgcgcc gtcgcgcggc ggtgctcctc
      601 ggaccgcgcg acgtctccga gggcgaggcg cgcggccgcc tgcgcctgct ccgcggtcgt
      661 cgcggcgtgc tcgagctcgt gcgcggcgtc catctccggg ctggtgcgca gggcgtcctg
      721 ggtgccgagc aggctgctgt ggtcgagggc cagctgcgct tcggcgtcgg tcacccgttc
      781 gagctcggcc tgcgcgcggg cgagctcctc gtcagcgacg ccctggtcgc ggccgacccg
      841 ctcgtactca ctctgcgcgg tacgcacgcc ctgcgccgcg cggcgcgaag ccacgcgcgc
      901 atattcggcg tagtggcgca gaaaggccgc ggcggcgtcg tgggtcgact cggcctcgag
      961 caaccggctc cggtcctcct cgaggctgcg gaacgattcg gcgacgtcgg cgatcacggc
     1021 ctggtcgagc ggtgcgagcg cctccgtcag ggccccggaa agcgcgcgcg cgtccggacg
     1081 cttcgacagc tgcggctggc gcagctggat gagcaggtcg accagcgcgc tgtaccggct
     1141 ctcgccgaga ccgaagagcg cctcgtccac cgcatggcgg tactcctccc gcgtgtcgta
     1201 gacgcggccg tggccctgga gctcgtcgcg caggcgctcg cgcgcgaggg cgaccccgtt
     1261 cgcgtccacg agccgaaggt cgccgatccg ccggtccgtc acgaagaacc agtgccgggc
     1321 gatcccgcga ccggtgacgg ccttcaggcc gcagcccagc gtggtgaagt gctcagtgcc
     1381 gtcgtcgtcg acgcggccga attcgaccca ggtatagccg agccgctcgc tgttcgggtg
     1441 atcgccgccg agcagcaggt tccactccat gcgcttcttc gggtcggcgt cgggctcgac
     1501 tcgctgcgcc gagagggacc catcgagcag gaacggcagc gtgagggcga ggaccttgga
     1561 cttgccggtc ccgttgttgc cgcgcagcag aagcctgccg tcatggaacc agaactgctc
     1621 gtcgtcgtag tagaaaaggt cgacgaggcc cagccgcagc ggctgccagc ggcgggtcgt
     1681 gggccggggt gccggcgagt cctgtctcat tgatcggttc ctctgctggt ttcgggcgcg
     1741 tcccgggccg cgcggatcgt gggtgctccg atggagaagc ggtgcagtgc cggccgcggc
     1801 gtgacggtcg tgccggccac gctcacgagg ccgaggtcca cgaggcgcct gacggcgtcc
     1861 gcgacgatgg cgttctcagc tcccggctgc tgtgcggtgc gccgccagta ggtcgagtac
     1921 tcgccagcga gcttgcgcac gaggtgacgg agctcgtcga tctcggtcgg gccggcgccg
     1981 gccaggtgct cggcgaccag tagcgtggcg tgcccgtcgg tgcccgactc gggcatccgc
     2041 acgtcagtca gctggtcgag cggatcgacc atcgcgacgc cctctgcgcg aatctcggcg
     2101 gtcagtcctg tgaactcggt gatgcgtcgc gtgatcgccg ctcgctgccg ggtcaggtag
     2161 acacgctcgg cctcgtcgag ctcgtcgtag tagacgaccg ggtcctcgag caagcggcgg
     2221 gtcagcgagt gccgcaacct acggttcgtc tcgtcctctg tgaaggcagg tggctccgcg
     2281 aacagcgcgc tctcgagctc gtcgattgcg agatcacctg aggggaagcc cggtgtgttc
     2341 acgaccaccg acgggccgtg gcgcaccacg agcagccgtg acagcactct gcggtcgacg
     2401 tcgtagagcg catctccgcc ggactggacg taggactcct cgtccccagc cacccgtgcc
     2461 agaaccccga gccccagcag caggcggatc gcggccgcca ggtcagaccg ctcatcacga
     2521 cccgcgagcg cgaactcgat cccctcgagc tccgtctgcc gggccaggag cacgacgttc
     2581 tcggcgagcc ggccgagaac gtcgtgctcc tggcccggca gacggagctc gaggggatcg
     2641 agttcgcgct cgcgggtcgt gatgagcggt ctgacctggc ggccgcgatc cgcctgctgc
     2701 tggggctcgg gg
//
LOCUS       DASRKH010000023         2076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_13059, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000023 DASRKH010000000
VERSION     DASRKH010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13059"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="VFC06_00385"
     CDS             complement(<1..646)
                     /locus_tag="VFC06_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015936961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZL80394.1"
                     /translation="MTTLDMLRDTMSGTVITADDPGYDEARAVYNFMIDRRPEAVAVC
                     TSTADVVAVVNYARDTGADLAVRGGSHSVPGFGTADGAVVADLSGMREVRVDADARVA
                     YVQGGATFNDVNVATGAHGLATPGGIISTTGVGGLTLGGGIGYLSRAYGLSIDNLVSV
                     EIVTADGQVRTASEEENTDLFWAIRGGGGNFGVVTEFRFQLHPVADVLAGPIFFE"
     gene            879..>2076
                     /locus_tag="VFC06_00390"
     CDS             879..>2076
                     /locus_tag="VFC06_00390"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZL80395.1"
                     /translation="MSAWVPPALPAWWAKSSGPAFVGRQGEFDELEEIWAAAERGMRQ
                     VVFVGGEPGAGKSRLVSEAALALDRAGVAVLVGHCLSDSPVAYQPFRDPVRSLIPAVD
                     GVIAAESQADIRDRLTSLVGLDGQDLGSVERAGQARDLYDAVVETLRGVAAIRPVALV
                     LEDLHWATRSTLQMLSYLVNHLTDARLLIVGTHRPTAPDRSEAAVETITDLYRNPGVH
                     RLDLEGLNTADVTEFLRLEAHLSSDRAKPLASQLRDETGGNPFFLQEICRDLGSDTGW
                     SAGAGSPRVPGTIRDAYASRLKRLGQSSREIVELSAVLGERIPVPLLLGASPIDEEST
                     LACLERAVEGGLLGSVPGGLDYRFAHALARQAVLDAIPPLRRSRLHARAGQSLEARPS
                     PGLTWTQ"
BASE COUNT          313 a          682 c          710 g          371 t
ORIGIN      
        1 gctcgaagaa gatgggccca gccaggacgt cggccacggg gtgcagctgg aatcggaact
       61 cggtcacgac tccgaagttg ccgccgccgc cccggatcgc ccagaagagg tcagtgttct
      121 cctcctcgga agcggtgcgg acctgtccgt ccgcggtcac gatctctacc gacaccaggt
      181 tgtcgatact cagcccgtac gcccgagaca aatagccgat gcctcctccg agtgtcaggc
      241 cgccaacgcc ggtcgtgctg atgatcccgc ccggggtggc caggccatgg gcacccgtag
      301 cgacgttgac atcgttgaag gtggcgccac cttgcacgta ggcgacgcgg gcgtcggcgt
      361 ccactcggac ctcacgcatc ccggagaggt cggcgacgac tgctccgtcc gcggtgccga
      421 agccgggcac gctgtgcgat ccaccccgca ccgccaggtc tgccccagtg tcgcgggcat
      481 agttgaccac tgcgacgaca tcggccgtcg atgtgcagac ggccacagct tcaggtcgac
      541 ggtcgatcat gaagttgtag acggcacgtg cctcgtcgta tccgggatcg tcggcggtga
      601 ttacggttcc gctcatggtg tcgcgaagca tgtccagggt tgtcatcagg gttctccttc
      661 tgaccggcag tgcggtcacc ggggcgtggg tgttgacccc aatgacggta cggagagccc
      721 ggcgcctgca catgaccaga agtcgtcgtc atggcagccg atgggtacct gtgcctgatc
      781 agaaatgcct atgccggtca gcccatttcg ggtcatggac agtctcccgc tgggcttgga
      841 ccctcgccag tgttcgtggc gttcgctacg ttagagacat gtctgcctgg gtccctcccg
      901 cgttgcccgc gtggtgggcc aagtcgagtg ggccagcttt cgtcggtcga cagggagagt
      961 tcgacgagct cgaggagatc tgggccgccg ccgagagggg tatgcgtcag gtcgtcttcg
     1021 tcggcggtga gcccggggct ggcaagtccc ggttggtcag cgaggcggcg ttggcactcg
     1081 acagggcggg ggtggccgtc ttggtcgggc actgcctgtc ggactcgccg gtcgcctacc
     1141 agcccttccg ggacccggtc cggtcgctga tccccgctgt cgatggggtg atcgccgctg
     1201 agtcacaggc cgacatccga gaccgactga cgtcgctggt ggggctcgac gggcaagatc
     1261 tcggttctgt ggagcgcgcc gggcaggctc gggacctcta tgacgctgtc gtggagacac
     1321 tacggggggt cgccgcgatc cggccggtcg cgctggtcct ggaggatctg cactgggcca
     1381 cgcggtccac gctgcagatg ctgtcctatc tggtcaacca cctgaccgac gcccggctgc
     1441 tgatcgtcgg cacccatcga ccaactgcgc ccgatcgatc cgaagcggcg gtcgaaacca
     1501 tcacggacct gtaccggaac ccaggcgtcc atcggcttga cctggagggg ctcaacacag
     1561 ccgacgtcac ggagtttctg cggttggaag cgcacctgtc gtcggatcgc gccaagccat
     1621 tggcctcgca gttgcgtgac gagaccggtg ggaacccgtt ttttctccag gagatctgcc
     1681 gcgacctggg ctccgacacc gggtggtctg ccggggcggg atcgccgcga gtcccgggga
     1741 cgatccgcga cgcgtacgcg tcgcgcctca agcggttggg ccagtctagt cgcgagattg
     1801 tcgagctctc cgcggtcctg ggcgagcgaa ttccggtgcc gctcctactc ggcgcttcgc
     1861 cgatcgatga ggagtcgacg ctggcgtgcc tcgagcgagc ggtcgagggt ggactgctcg
     1921 ggtccgtccc tggcgggctg gactatcggt tcgcccacgc actggcacga caggccgtcc
     1981 tcgacgcgat ccccccactt cgcaggtcgc ggctccacgc ccgcgcgggc caatccctgg
     2041 aggcccgccc gtcaccgggg ctgacctgga cccaac
//
LOCUS       DASRKH010000024         2067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_61806, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000024 DASRKH010000000
VERSION     DASRKH010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61806"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1625
                     /locus_tag="VFC06_00395"
     CDS             <1..1625
                     /locus_tag="VFC06_00395"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HZL80396.1"
                     /translation="GSGRRGDGEHPPAAVLHGRGMLAVSAAARAAGVRRGMRKRLAQR
                     ACPELAILTHDEGRDAREFESVAAAAEQVVAGVEISRPGLLMIPSEGAARFHGSEEAL
                     AEALVTRVAEDAGCESAVGIADGLLAAVMAARANALVERGQSHDFLAPLPVDTLVHAA
                     MERSQRTEVEDLVSVLARLGLTTLGDFTALPTADVVARFGHVGAWAQRMARGEDVAPP
                     VLRRAEGDIGVEYLFEDPAERVEQLALVASYVAEQLDAALLAAASRCSRVRIGARTVT
                     GDELVRVWRTDVGARSGAFSKHMADRVRWQLEGWLSGTAAGPQPAPLTALMLTAEDVV
                     PLGSEQSFLWGGTSGADARAHRALERVQGLLGAEGVLAVAEQGGRTPRDRVHALAWGQ
                     EGEPPRRVSHPWPGRIPDPAPATVLTVPLEVHVLDAGGSPVRVDSRLAVSAPPTWVRL
                     QADPRDSVARAAHRHPSSGHVGGDDGPVWTSPLPVEAWAGPWPIVERWWSGEASRRAF
                     LQLAMRGEDGQTEAALLVALHEGRWMLEAIYD"
     gene            1651..>2067
                     /locus_tag="VFC06_00400"
     CDS             1651..>2067
                     /locus_tag="VFC06_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HZL80397.1"
                     /translation="MTECVDYAELHAHSAFSFLDGASQPEEMAAEAGRLGLSAMALTD
                     HDGLYGVVRFANAARSVGLPSIFGAELHLSAPDGKAGAPVLDAPTGTPDPRGTHLVVL
                     ARGAGGYARLSRTIGMAHLAAGEKGLAHYTLEGIAEA"
BASE COUNT          278 a          694 c          793 g          302 t
ORIGIN      
        1 gcggctcggg ccgccgcgga gacggcgagc atccccctgc cgcggtattg cacggcaggg
       61 ggatgctcgc cgtctccgca gcggcccgcg ccgcgggggt gcgcaggggg atgcgcaagc
      121 gcctcgccca gcgggcctgc ccggagctcg cgatcctgac tcacgacgag ggtcgggacg
      181 cccgcgagtt tgagtccgtc gccgctgcgg cggagcaggt ggtcgcgggg gtcgagatct
      241 ctcgcccggg cttgctgatg atcccctcgg agggcgcagc gcgttttcac ggttccgaag
      301 aggcgctcgc ggaggctctc gtgacccggg tggcggaaga cgcggggtgc gagtccgccg
      361 tgggcatcgc cgacggacta ttggccgccg tgatggcagc cagggcgaac gcgttggtcg
      421 agcggggcca gtcacacgac tttttggcgc ctcttcctgt ggacaccctg gtgcacgccg
      481 ccatggagcg ctcccagcgc accgaggttg aggacctcgt cagcgtgctg gcgcgcctgg
      541 gcctgaccac cctgggtgac ttcaccgcgc ttcccacggc cgatgtcgtg gcccgttttg
      601 ggcacgtggg cgcgtgggcg cagcgcatgg ccaggggaga ggacgttgcc cctcccgtcc
      661 tgaggcgagc cgagggcgac atcggcgtcg agtatctgtt cgaggatcca gccgaaaggg
      721 tggaacaact cgcactggtg gccagttatg tggccgaaca actcgacgcc gcgctgctgg
      781 ccgccgcctc gcggtgctcc cgcgtgcgga tcggcgcccg caccgtgacg ggggacgaac
      841 tggtcagggt gtggcgcacg gatgtgggag cccgctcggg agccttctcc aagcacatgg
      901 cggacagggt gcgctggcag cttgagggat ggctgtcagg caccgccgcg gggccgcagc
      961 ccgcacccct cacagcgctg atgctcacgg ccgaggacgt ggttccgctt ggatccgagc
     1021 agtccttcct gtggggtggc acgtccgggg ccgacgccag ggcgcatcgt gcgttggaaa
     1081 gagtgcaggg gctgttgggc gcggagggcg tgctcgccgt ggcggagcaa ggcggacgca
     1141 cgccccgcga ccgggtgcat gcgctcgcgt ggggccaaga gggcgagcct ccccggcggg
     1201 tcagccaccc gtggccgggc cgcatccccg atccggcgcc ggcgacagtg ctgaccgtgc
     1261 cgctcgaggt tcacgtgctc gacgcgggcg gaagccccgt gcgggtggac agtcggctcg
     1321 ccgtgagcgc cccacccacc tgggtgcgcc tgcaggccga cccgcgcgac agcgtggccc
     1381 gcgcggcgca tcgtcacccg tcgtctggtc atgtgggagg agacgacggc cccgtgtgga
     1441 cgtcgcccct gcccgtcgag gcgtgggcgg gcccgtggcc gatcgtggag aggtggtggt
     1501 caggggaggc gtcccggcga gccttcctgc aactggcgat gcgaggagaa gacggtcaga
     1561 cggaggcggc actactagtg gcacttcacg aggggcgctg gatgttggag gccatctatg
     1621 actgaggtgc ggcatgactg aggtgcggcg atgacggagt gcgtcgacta cgcggaactg
     1681 cacgcccact cggccttcag ttttctcgac ggcgccagcc aacccgagga gatggccgcc
     1741 gaggccgggc gcctggggct gtccgcgatg gccctcaccg atcacgacgg cttgtacggg
     1801 gtggtgcgct tcgccaacgc cgcccgctcg gtgggcctgc ccagcatctt cggcgcagaa
     1861 ctgcacctgt ccgcgccgga tggcaaggcg ggtgcccccg tgctggacgc gcccaccggc
     1921 acccccgacc cgcgcggcac ccacctcgtg gtgctcgcgc gcggcgctgg cggatacgcc
     1981 cgactttccc gcaccatcgg catggcgcac ctggccgccg gcgagaaggg cctggcgcac
     2041 tacacgcttg aggggatcgc agaggcg
//
LOCUS       DASRKH010000025         2350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_8189, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000025 DASRKH010000000
VERSION     DASRKH010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8189"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..172
                     /locus_tag="VFC06_00405"
     CDS             <1..172
                     /locus_tag="VFC06_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003786651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HZL80398.1"
                     /translation="DRVVTHILAYEGTEENPAAWYWFEGNFASYEANKVERLGEEAAR
                     PHRVTYRKLTRD"
     gene            complement(202..369)
                     /locus_tag="VFC06_00410"
     CDS             complement(202..369)
                     /locus_tag="VFC06_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HZL80399.1"
                     /translation="MTPTAITLLALSAVLVWGGLIASTIHLIRHPEVSAYPAGGEDDE
                     RPEHAVIVHDT"
     gene            complement(371..1945)
                     /locus_tag="VFC06_00415"
     CDS             complement(371..1945)
                     /locus_tag="VFC06_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HZL80400.1"
                     /translation="MSARTEQSTAAPREQFSGQTGFILSAIGSAVGLGNIWRFPGVAY
                     EHGGGAFLVPYLVALVTAGIPILFLDYAIGHRFRGSAPTALRRLGGRWGRWTESLGWF
                     QVSLSFVIALYYTAIIGWALSYFVFSFDLRWGDDPAGFLLGDYLQASEEVSLTGDIVA
                     SVAVPLAIVWIAAIAALALGVTRGLERVNVVVMPLLALTFGAVVVRALFLPGAADGLN
                     AFFTPDWDALSDPGVWIAAYGQIFFSLSIAFGIMITYASYRRRRSNLTGPGLVVAFAN
                     SSFELLAGIGVFATLGFFAFQQGIPVSELESISGIGLSFITFPAILSEMPGGAAVGAL
                     FFGTLTLAGFTSLLSILQVTSAAVQEKFGLTPRSGAVAIGTVSALLSFALFSTTSGLY
                     ALDATDHWVNTIGVVASAVIMSVLTVWVLRRGPELLHHLNTVSTVRLGRVWLALVGMV
                     VPAVLGYMLVSGIVTVATEGYEGYPTGFLLVMGWGALIAVGVIAAVATALPWRRNKDD
                     VEPWPPYPAAAATEEN"
     gene            complement(2070..>2350)
                     /locus_tag="VFC06_00420"
     CDS             complement(2070..>2350)
                     /locus_tag="VFC06_00420"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HZL80401.1"
                     /translation="EPHWVYGKYDVKNLTDPEGTLGTAESLTTYSRLGFADDFPEVNA
                     WMAAFKMDGALLADLENNLQGADSEADYDGIVTDWIVANQEWVDSLTA"
BASE COUNT          463 a          764 c          805 g          318 t
ORIGIN      
        1 cgaccgcgtg gtgacgcaca tcctcgccta cgagggcacc gaggaaaacc ccgctgcctg
       61 gtattggttc gagggcaact tcgcgtctta cgaggccaac aaggtggagc gcctcggcga
      121 agaggcagcc cggcctcacc gcgtcacgta ccgcaagctc acgcgggact gagtcgggga
      181 cgccgtcggc gcgagcggtg ctcaggtgtc gtggacgatg acggcgtgct cggggcgctc
      241 gtcgtcctca ccgccggcgg ggtaggcgga cacctcgggg tgtctgatga ggtgaatggt
      301 gctggcgata agtccgcccc acaccagtac ggccgacagt gcgagcagcg tgattgccgt
      361 gggggtcatg tcagttctcc tccgttgcgg cggccgccgg gtagggcggc cacggctcga
      421 cgtcgtcctt gttgcggcgc cacggcagag ccgtcgccac cgcggcgatc acgccgacgg
      481 cgatgagtgc gccccagccc atcacgagca ggaaccccgt cgggtagccc tcgtagccct
      541 cggtcgcgac tgtcacgatg ccactcacca gcatgtagcc gagcacggcc ggcaccacca
      601 tcccgaccag ggccagccac acccgcccga gtcgcacggt cgacaccgta ttgaggtggt
      661 gcagcagttc tggtccgcgg cgcagcaccc atacggtgag caccgacatg atcacggccg
      721 aggcgaccac gccaatcgtg ttgacccagt ggtccgtcgc atcgagcgcg tagaggccgg
      781 acgtggtcga gaacagcgcg aacgacagga gcgccgagac ggttccgatc gccaccgcgc
      841 ccgagcgcgg cgtgagcccg aacttctcct gcaccgcggc cgacgtcacc tgcaggatcg
      901 acaacagcga ggtgaagccc gcgagcgtga gagtgccaaa gaacagggcc ccgacggcag
      961 ccccgccggg catctccgac aggatcgccg ggaaggtgat gaaggacagc ccaatgccgg
     1021 agatggactc gagttcagaa acagggattc cctgctggaa cgcgaagaag ccgagcgttg
     1081 cgaagacgcc gatgcctgcc aacagctcga acgaggagtt ggcaaaggcc accaccaggc
     1141 ccgggccggt gaggttggac cggcggcgcc tgtaggaggc gtacgtgatc atgatgccga
     1201 acgcgatcga cagcgagaag aatatctggc cgtacgcggc aatccacacg cctgggtcgg
     1261 acagcgcgtc ccagtcgggg gtgaagaagg cattcaggcc gtcggcagcg cctgggagga
     1321 atagcgcgcg caccaccacg gcaccgaacg tcagggctag cagcggcatc acgacgacgt
     1381 tgacgcgttc gagcccccga gtcactccga gcgcgagcgc cgcgatcgcg gcaatccaca
     1441 caatcgcgag gggaacggcc acggacgcaa cgatgtcgcc ggtgagggac acctcttctg
     1501 acgcctggag gtagtcgccc agaaggaagc cagcagggtc gtcgccccag cgcaggtcga
     1561 acgagaagac gaagtagctg agcgcccagc cgatgatggc cgtgtagtac agggcgatca
     1621 cgaaggatag ggagacctgg aaccagccga gcgactctgt ccaccggccc cagcgcccgc
     1681 ccagacggcg cagcgccgtg ggggccgaac cgcgaaagcg gtggccgatc gcgtagtcga
     1741 gaaagaggat cgggattcca gccgtgacaa gcgccacaag atacggaacc aggaaggcgc
     1801 caccgccatg ctcgtaggcc acgccaggga agcgccagat gttgcccagg cccacggcag
     1861 agccgatggc cgacaggatg aagcccgtct gtccggagaa ctgctcgcgc ggcgcggctg
     1921 tggactgctc ggtgcgtgca gacatttcac tccctcgtga tttcgccgcc acactagcag
     1981 cggccgccgc acactcatgc aacacccgga gacggcaaag cgccggccac ccgaaggtga
     2041 ccggcgcttt gccgtctgaa ggggagagac tacgcggtga gcgagtccac ccactcctgg
     2101 ttcgcgacga tccagtcagt cacgatgccg tcgtagtcgg cctcgctgtc agcgccctgg
     2161 agattgttct ccaggtcggc cagcagggcg ccgtccatct tgaaggcagc catccacgcg
     2221 ttgacctcgg ggaagtcatc ggcaaagccg aggcgtgagt acgtcgtgag ggactcggcg
     2281 gtgccgaggg tgccctcggg gtccgtgagg ttcttgacgt cgtacttgcc gtagacccag
     2341 tggggctccc
//
LOCUS       DASRKH010000026         2881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_3794, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000026 DASRKH010000000
VERSION     DASRKH010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3794"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VFC06_00425"
     CDS             <1..222
                     /locus_tag="VFC06_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80402.1"
                     /translation="ELTKHGITSPGVQRAGMSKILGRKITSRAELTEADGLAVIGDLQ
                     ARAVPTPAVEPDDGPGPSDADYDAMEASS"
     gene            219..386
                     /locus_tag="VFC06_00430"
     CDS             219..386
                     /locus_tag="VFC06_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80403.1"
                     /translation="MTAWTSCAHPGCGRTVDVKDVFCPEHMPPKRVEYSLDETSLRAI
                     AELVALLKDHS"
     gene            463..705
                     /locus_tag="VFC06_00435"
     CDS             463..705
                     /locus_tag="VFC06_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80404.1"
                     /translation="MTAAESREVRFLEWLSGRVIVRLAVLAKRRGDDHEATHCAAALA
                     VGNTGAHHEPCMLVTHTGGRAVIELARRLAEAGGSR"
     gene            806..976
                     /locus_tag="VFC06_00440"
     CDS             806..976
                     /locus_tag="VFC06_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80405.1"
                     /translation="MIRALRVAVILLAVLAVSIVLAPSPGFGGRGVVAVAVAIVVLRV
                     GARAERGRVGAK"
     gene            973..1152
                     /locus_tag="VFC06_00445"
     CDS             973..1152
                     /locus_tag="VFC06_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80406.1"
                     /translation="MKAEIEDTIAYQGTCDAGDCEAETLAVVLTPDGWTAMCAEHAVI
                     EARGAILSESMNGHS"
     gene            1149..1466
                     /locus_tag="VFC06_00450"
     CDS             1149..1466
                     /locus_tag="VFC06_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80407.1"
                     /translation="MSVPISEECRSFGGRGRDPDWDRDRKSTPQHVLYQRHDYGDGVV
                     YEHTSISNWSTPDDALASRIKRLKNTGTKYSTIGRTVTFEDANPERHTVTLTYADPNE
                     ARP"
     gene            1463..1819
                     /locus_tag="VFC06_00455"
     CDS             1463..1819
                     /locus_tag="VFC06_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80408.1"
                     /translation="MSVAQPVMHSLPFEGYPRRWLSVDLLDIARQRAAGWPDFHPEDF
                     CHRCGAANVCWFTDSTTWNDVMEDETRWNGILCVPCFIALTEDRVGGCAWRLTRDEYG
                     SEVLTLLHVGDPDGAS"
     gene            1816..1989
                     /locus_tag="VFC06_00460"
     CDS             1816..1989
                     /locus_tag="VFC06_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80409.1"
                     /translation="MSADDTTLTPESRRMVVYYRKRAEECRTNAVTWDALADELEAFG
                     ETGEHDDGQGALL"
     gene            1986..2264
                     /locus_tag="VFC06_00465"
     CDS             1986..2264
                     /locus_tag="VFC06_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80410.1"
                     /translation="MSEPTLFDRPVPADHDTDTTIVHALMPWMGLACGGDVLTISRAG
                     RAAGTRTITTHVGAHWGEVTCPDCLALGDLSALARNSQRPERLATVTP"
     gene            2261..2611
                     /locus_tag="VFC06_00470"
     CDS             2261..2611
                     /locus_tag="VFC06_00470"
                     /inference="COORDINATES: protein motif:HMM:NF014520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HZL80411.1"
                     /translation="MSTLIGGRCAEVDPAVWFPEKGQTNTAAKAICHRCDIEAACLAA
                     AIADPELEGVWGGTSQRERQALRGSSLPRIRPINHGTEGGYMTHLRRGEPACPVCRDA
                     TTSAHRRRRAERVA"
     gene            2608..>2881
                     /locus_tag="VFC06_00475"
     CDS             2608..>2881
                     /locus_tag="VFC06_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80412.1"
                     /translation="MTPARFHAAPDVVVREGGSDRADWRDEGVCVTWTAWPADPWYPG
                     EHATSTDPVKRAAAWAKGRELCASCPITGLTGPCLEDALAHESGSSY"
BASE COUNT          484 a         1028 c          940 g          429 t
ORIGIN      
        1 gagctgacga agcacggaat cacgtccccc ggggtgcaac gcgctgggat gtcgaagatc
       61 ctcggccgca agatcacgtc ccgcgccgag ttgaccgagg ctgatgggct ggccgtgatc
      121 ggcgacttgc aggcgcgggc cgttcctacc cccgccgtcg agcctgacga tggtcctggc
      181 ccgtccgatg ctgactacga cgcgatggag gcgtcgtcat gaccgcctgg acctcgtgcg
      241 cccaccccgg atgtgggcgg acggtggacg tcaaggacgt cttctgcccg gagcacatgc
      301 cacccaagcg agtcgaatac tccctcgacg agacgagcct ccgggcgatc gccgaactcg
      361 tcgcgctcct gaaggaccac tcgtgatcgc cgtcgactgg gtcctcatca cccttgccgc
      421 cctgttcgtc gtgtaccagg tgcgccgtcc gatgaggcgg ccatgaccgc cgccgagagt
      481 cgcgaggtcc gtttccttga gtggctgtcg ggccgcgtca tcgtccgact cgctgtcctg
      541 gcgaagcggc gcggggatga ccacgaggcc acccactgcg ccgccgcttt ggcggtcggc
      601 aacacgggcg cccaccacga gccgtgcatg ctcgtgactc acaccggcgg gcgtgccgtg
      661 atcgaactag cgcggcgact ggccgaagcg ggtggctccc gatgaccgtc gaccgcgccc
      721 tcaccctcgt cgccgcgctc ctgcttacgg gtggcctggt cctcctcgct gccggcatct
      781 gggccgggga agacaggggt gtccggtgat ccgcgccctt cgtgtcgccg tcatcctcct
      841 ggccgtcctc gccgtgtcca tcgtcctggc cccgtcgcct gggttcggtg ggcggggcgt
      901 ggtcgccgtc gcagtcgcca tcgtcgtcct cagggtcggc gcacgagccg agaggggacg
      961 ggtgggtgcg aagtgaaggc cgagatcgag gacacgatcg cctaccaggg cacatgcgac
     1021 gccggtgact gcgaagccga aacgttagcc gtcgtcctca cgccagacgg atggacggcg
     1081 atgtgcgcag agcacgccgt catcgaggcg cgcggcgcca ttctctccga gtccatgaat
     1141 ggtcactcat gagcgtaccg atcagcgagg aatgccgatc cttcggcggg cgcggtcgtg
     1201 acccggactg ggaccgggac cggaaatcga caccccagca cgtcctatac caacggcacg
     1261 actacggcga tggcgtggtc tacgagcaca ccagcatctc caattggtcc acgcccgacg
     1321 acgccctcgc cagccgaatc aagcgtctca agaacacagg gacgaagtac agcaccattg
     1381 ggcgcacggt cacatttgag gacgcgaatc ccgaacgaca caccgtgacg ctcacctacg
     1441 ccgacccgaa cgaggcccgg ccatgagcgt cgcccagcct gtgatgcact cgttgccatt
     1501 cgagggctac cccaggcgat ggctatccgt cgacctgctc gatatcgcca ggcaacgcgc
     1561 tgcagggtgg ccggacttcc accccgaaga cttctgccac cgctgcggcg cggccaacgt
     1621 gtgctggttc acggactcga cgacatggaa cgacgtcatg gaggacgaga cccgctggaa
     1681 cggcatcctc tgtgtcccgt gcttcatcgc gctcactgag gaccgcgtcg gcggctgcgc
     1741 ctggcgtctg acgcgggacg agtacggcag cgaagtcctc accctcctgc acgtcggtga
     1801 cccggatggt gcctcatgag cgccgacgac accacgctca cccctgagtc gcgccgcatg
     1861 gtcgtctact accggaagcg cgccgaggaa tgccgcacga acgccgtgac gtgggacgcg
     1921 ctagccgacg aactcgaagc tttcggtgag accggcgaac acgacgacgg acagggagcg
     1981 ctgctgtgag cgagccgacc ctgttcgacc gccctgtacc cgccgaccac gacacggaca
     2041 cgacgattgt ccacgcgctc atgccgtgga tgggcctcgc gtgcggcggc gacgtactca
     2101 ccatctcccg tgcgggacgc gcagctggca ctcggaccat cacaacccac gtcggtgccc
     2161 actggggcga agtgacctgt cccgactgcc tagcgctcgg cgacctgtca gcactcgccc
     2221 gcaactccca gcggccagaa cgactcgcga cggtgacccc gtgagcaccc tcatcggcgg
     2281 cagatgcgcc gaggtcgacc ctgctgtctg gttccccgag aaagggcaga ccaacacagc
     2341 cgccaaggcg atctgccacc gctgcgacat cgaagccgcc tgcctcgccg ccgcgatcgc
     2401 tgaccccgaa cttgagggcg tctggggtgg cacctcgcaa cgggaacgcc aggcgctgag
     2461 gggctctagc ctccccagga tccgcccgat caaccacggc accgagggtg gatacatgac
     2521 ccacctgcgc cgaggcgagc ctgcgtgccc ggtgtgcagg gacgcgacga cgagcgccca
     2581 ccggcggcgg cgagcggagc gtgtcgcatg actcccgccc gcttccatgc cgcccccgat
     2641 gtcgtcgtcc gtgagggcgg cagcgaccgg gccgactggc gggacgaggg cgtgtgcgtc
     2701 acctggaccg catggccggc cgacccctgg taccccggcg agcacgccac gtccaccgac
     2761 cccgtgaaac gcgccgccgc atgggccaag ggtcgcgaac tgtgcgcatc gtgcccgatc
     2821 acgggtctca cgggcccgtg cctcgaagac gcgctcgccc atgagtcggg cagctcgtac
     2881 t
//
LOCUS       DASRKH010000027         2259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_26702, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000027 DASRKH010000000
VERSION     DASRKH010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26702"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="VFC06_00480"
     CDS             <1..603
                     /locus_tag="VFC06_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013115904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZL80413.1"
                     /translation="PDATLPDSAIVPVNRSDESGTTDNFTKYLAAAAPEAWTYEPDGV
                     WPISGTQSGAQTSGVLEVVNGAEGTIAYVDASRAGTLGTVAVGVGDEFVPFSPEAAAK
                     VLEVSSPTADATDLRLTFDLARDTAESGTYPVVLVSYLLACSQYDNEADAANVAAYLS
                     YIASPEGQTLAASPEVAGIAPISDALRTQVDAAIAQITAG"
     gene            683..1654
                     /gene="pstC"
                     /locus_tag="VFC06_00485"
     CDS             683..1654
                     /gene="pstC"
                     /locus_tag="VFC06_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HZL80414.1"
                     /translation="MAAPTDQRVTPEVIDLRPPSNTGNRVFAGLSAGAGWAIMLTLAA
                     VALFLFVRAAPAFTSSAEEIAGVSILRGQSFWGFVIPLLFGTLLSSVIALLLAVPLSI
                     GIALFISHYAPRRLAQTLGYIVDLLAAIPSVVFGLWGARWLMGTIDPVFQWLSDNLGF
                     LPFFQSYVPPARNIMTASLVLAVMILPIITATIREVFLQTPRLHEEASLALGATRWEM
                     VRQAVLPFARSGIISASMLGLGRALGETMAVLMILSPGFLMNFFLLKPGQHQTIAANI
                     ANQFPEASGLGVDVLIATGLMLFIITFLVNVAARAIIARRAEFSGAN"
     gene            1654..>2259
                     /locus_tag="VFC06_00490"
     CDS             1654..>2259
                     /locus_tag="VFC06_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter, permease protein
                     PstA"
                     /protein_id="HZL80415.1"
                     /translation="MTALAPQRIGQRLPKWITWAVLAGSLAVGYGINLTAGAFSLLGG
                     GFLGAIIYVIAITAISWVREGERWAKDRLASALVVAAFVVVMIPLVSLIAMVVVEGSK
                     RFSFTFLNTNMVGVYGETELGGVYHAIVGTLLITGLASLISIPIGLFTAIYLVEYRGG
                     RIARWITFLVDVMTGIPSIVAGLFAYALFAAIFGPGYKSGLI"
BASE COUNT          308 a          844 c          702 g          405 t
ORIGIN      
        1 cccgacgcca cgctgcccga ctcggcgatc gtgcccgtga accgctcgga cgagtccggc
       61 accaccgaca acttcaccaa gtacctcgcc gccgccgctc ctgaggcctg gacctacgag
      121 cccgacggcg tgtggccgat ctccggcacg cagtccggtg cgcagacctc cggagtcctc
      181 gaggtcgtga acggtgccga gggcaccatt gcctacgtcg acgcgtcccg cgccggcacc
      241 ctgggcacgg tcgcggtcgg tgtgggcgac gagttcgtgc cgttctctcc cgaggccgcc
      301 gccaaggtgc tcgaggtctc ctcgcccacc gcggatgcca cggacctgcg cctgaccttc
      361 gacctggccc gtgacaccgc ggagtccggt acctacccgg tcgtgctggt ctcctacctg
      421 ctcgcgtgct cgcagtacga caacgaggcc gacgccgcca atgtggcggc ctacctctcg
      481 tacatcgcga gccctgaggg ccagacgctg gccgccagcc ccgaggttgc cggaatcgcc
      541 ccgatctcgg acgcgcttcg cactcaggtg gacgccgcca tcgcccagat cacggccggc
      601 tgacaaccgg agccaaaggg cgggtcgagc ctccggccga cccgcccttc gccctgtact
      661 ttggaggcgt gaaggaggag ccatggcagc accaaccgac caacgcgtga cgccggaggt
      721 cattgatctc aggccgccca gcaacacggg gaaccgggtc ttcgcgggac tctcggccgg
      781 ggcagggtgg gcgatcatgc tgacgctcgc cgccgtcgcc ctcttcctct tcgttcgggc
      841 ggcaccggca ttcacctcca gcgccgagga gatcgccggg gtcagcatcc tgcgggggca
      901 gagcttctgg ggcttcgtca taccgctcct cttcggcacg ctgctctcct cggtcatcgc
      961 actcctcctc gcggtgccgc tctccatcgg catcgcgctg ttcatctcgc actacgcccc
     1021 ccgccggctc gcccagacac tcggctacat cgtggacctg ctcgccgcca tcccctccgt
     1081 ggtcttcggc ctgtgggggg cgcgctggct gatgggcacc atcgacccgg tgttccagtg
     1141 gctgagcgac aacctgggct tcctcccctt cttccagtcg tacgtcccgc cggcgcgcaa
     1201 catcatgacg gcctccctgg tcctcgcggt catgatcctg ccgatcatca cggcaaccat
     1261 ccgcgaggtg ttcctccaga cgccgcgcct gcacgaggag gcctcgctgg ccctgggggc
     1321 cacgcggtgg gagatggttc gccaggcggt cctgcccttc gcgcgctccg gcatcatctc
     1381 ggcgtccatg ctcgggctcg ggcgggccct gggtgagacg atggctgtgc tcatgatcct
     1441 gtccccggga ttcctcatga acttcttcct gctcaagccg ggccagcacc agaccatcgc
     1501 ggcgaacatc gcgaaccagt tccccgaggc gtccggactg ggcgtcgacg tgctcatcgc
     1561 cacgggcctc atgctgttca tcatcacgtt cctggtgaac gtggcggccc gcgcgatcat
     1621 cgcccgccgc gctgagttct caggagccaa ctgatgaccg ccctcgcccc gcagcgtatc
     1681 ggccagcgcc tgccgaagtg gatcacctgg gcggtcctcg ccggctcact cgccgtcggg
     1741 tacggcatca acctcaccgc gggcgcgttc agcctgctcg gcgggggatt cctcggcgcg
     1801 atcatctacg tgatcgcgat caccgccatc tcatgggtcc gcgagggcga gcggtgggcg
     1861 aaggaccggc tcgcgagcgc gctcgtcgtc gccgccttcg tcgtcgtgat gatcccgctc
     1921 gtctccctca tcgcgatggt ggtggtggag ggctccaagc gcttcagctt caccttcctc
     1981 aacaccaaca tggtgggcgt ctacggcgag accgagctgg gcggggtgta ccacgcgatc
     2041 gtcgggaccc tgctcatcac cggtctggcc tcgctcatct ccatccccat cggcctcttc
     2101 accgcgatct acctcgtgga gtaccgcggt gggcgcatcg ccaggtggat caccttcctc
     2161 gtggacgtga tgacgggcat cccgtcgatc gtcgccggcc tgttcgccta cgcgctcttc
     2221 gccgcgatct tcggtccggg ctacaagtcg ggcctgatc
//
LOCUS       DASRKH010000028         2130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_26675, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000028 DASRKH010000000
VERSION     DASRKH010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26675"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1374)
                     /locus_tag="VFC06_00495"
     CDS             complement(<1..1374)
                     /locus_tag="VFC06_00495"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HZL80416.1"
                     /translation="MEGPEIKFAEATIDNGSHGTRTVRFETGRLARQAAGSAVVYLDE
                     DMMILSATTVGKHPKDQLDFFPLTVDVEERQYAAGKIPGSFFRREGRPSTDAILACRL
                     IDRPLRPAFVKGLRNEVQIVETILALHPDDAYDVVAINAASMSTQISGLPFSGPIGGV
                     RIALVGGQWVGFPRYSELENAVFSMVVAGRVVGDDVAVMMVEAEATDAAWNLIQGGAQ
                     APTEEVVAGGLEAAKPFIRALCEAQASLAAEAAKPTAEFPRFPDYEQDVLDVVESRVR
                     AEIDEAIRIGDKQERETRLEGIRHAVLAELAEQFAGRDKEISAAVRSVTKTMVRARVL
                     TEGKRMDGRGLRDIRTLSAEVEVLPRVHGSALFERGETQILGVTTLNMLRMEQQIDSL
                     SPVTRKRYMHNYNFPPYSTGETGRVGSPKRREIGHGALAERALLPVLPAREEFPYAIR
                     QVSEALSS"
     gene            complement(1545..1814)
                     /gene="rpsO"
                     /locus_tag="VFC06_00500"
     CDS             complement(1545..1814)
                     /gene="rpsO"
                     /locus_tag="VFC06_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010079122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HZL80417.1"
                     /translation="MPLAPDVKQKIMSEYATHEGDTGSPEVQIALLSQRIRDLTEHFK
                     THKHDHHSRRGLMLLVGQRRRLLGYLQEVDIERYRSLIERLGLRR"
     gene            complement(1949..>2130)
                     /locus_tag="VFC06_00505"
     CDS             complement(1949..>2130)
                     /locus_tag="VFC06_00505"
                     /inference="COORDINATES: protein motif:HMM:NF013821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HZL80418.1"
                     /translation="TDLDLYDHVVTVEFTNWIRPMLTFSGVEPLLERMAEDVRETADL
                     LGVPRPAAPHHAPPA"
BASE COUNT          308 a          706 c          777 g          339 t
ORIGIN      
        1 ggagctcagc gcctcggaga cctgccggat cgcgtacggg aactcctccc gagccggcag
       61 caccggcagc agcgcccgct cggccagcgc gccgtggccg atctcgcggc gcttgggcga
      121 accgacccgg ccggtctccc cggtcgagta cggcgggaag ttgtagttgt gcatgtagcg
      181 cttgcgcgtc accggcgaga gcgagtcgat ctgctgctcc atgcgcagca tgttgagggt
      241 ggtgacgccc aggatctggg tctcgccacg ctcgaacaac gccgagccgt gcacgcgggg
      301 aagtacctcg acctcggcgg agagcgtgcg gatgtcccgc aggccgcgcc cgtccatgcg
      361 cttgccctcc gtcaggacgc gagcacggac catcgtcttg gtgaccgagc ggacggccgc
      421 cgagatctcc ttgtcccggc cggcgaactg ctcggcgagc tccgcgagga ccgcgtggcg
      481 gatcccctcg aggcgggtct cgcgctcctg cttgtcgccg atgcggatgg cctcgtcgat
      541 ctccgcgcgg acgcggctct cgacgacgtc gaggacgtcc tgctcgtagt cggggaagcg
      601 ggggaactcc gccgtcggct tcgcggcctc ggccgccagg ctggcctgcg cctcgcagag
      661 cgcgcggatg aacggcttgg ccgcctcgag gccgccggcg acgacctcct cggtcggcgc
      721 ctgggcgccg ccctggatga ggttccaggc ggcgtcggtc gcctcggcct cgaccatcat
      781 gacggcgacg tcgtcaccga cgacccgtcc ggccacgacc atagagaaga ccgcgttctc
      841 cagctcggag tagcgcggga agcccaccca ctggccgccg acgagggcaa tgcgcacccc
      901 accgatcggg cccgagaagg gcagcccgga gatctgggtc gacatcgacg cggcgttgat
      961 ggcgacgacg tcgtacgcgt cgtcggggtg gagcgccagg atcgtctcga cgatctggac
     1021 ctcgttgcgc agccccttca cgaaggcggg gcgcagcggc cggtcgatga gccggcacgc
     1081 caggatcgcg tccgtggacg ggcggccctc gcggcggaag aacgagccgg ggatcttgcc
     1141 cgcggcgtac tggcgctcct ccacgtccac cgtgagcggg aagaagtcta actggtcctt
     1201 cgggtgcttg cccaccgtgg tggcggacag gatcatcatg tcctcgtcga ggtagacgac
     1261 ggcggagccg gcggcctggc gggcgaggcg cccggtttcg aaccggacgg tgcgggtgcc
     1321 gtgggaacca ttatcgatgg ttgcctcagc aaatttgatt tcgggaccct ccatggggtt
     1381 tccttccgtt ctgcttcgac cggccggccg gctagccatc gatcgaagct cacggacgaa
     1441 agtttccgga agccaccacc gaggaccgaa ccgtgggcgc ggtactggtt gtgatgcggg
     1501 tgcgccaccg gcccggaccg ctcggtccgg gccggggcgg tggtctagcg acgcaggccg
     1561 aggcgctcga tcagcgagcg gtaacgctcg atgtcgacct cctggaggta cccgaggagg
     1621 cggcggcgct gccccacgag gagcatgagc ccgcgacggg agtggtggtc gtgcttgtgg
     1681 gtcttgaagt gttcggtaag gtccctgatc cgctgcgaga gcagcgcgat ctggacctcg
     1741 ggggaaccgg tgtcaccctc gtgggtggcg tactcggaca tgatcttctg cttgacgtca
     1801 ggtgcgaggg gcacgagttc tccttcttgt cgctgcacgg agcgccgggg catgttcacc
     1861 cgggcatgac gcaaccgtgg ccggtctaac ggccttgcca gcttaccagc ccgccccacc
     1921 gcccggcgcc ggggtgaggt gaagcggctc acgcgggcgg tgcgtggtgc ggcgcggcgg
     1981 ggcgcggcac ccccaggagg tcggccgtct cgcggacgtc ctcagccatc cgctcgagca
     2041 gcggctcgac gccgctgaag gtcagcattg ggcggatcca gttggtgaac tcgaccgtga
     2101 ccacgtggtc gtagagatcg aggtctgtct
//
LOCUS       DASRKH010000029         2001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_8123, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000029 DASRKH010000000
VERSION     DASRKH010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8123"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="VFC06_00510"
     CDS             complement(<1..987)
                     /locus_tag="VFC06_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZL80419.1"
                     /translation="MRSAPDDATEAPVGPATPVADAPTAGSPLWIANFRRYAGAQLLG
                     SSGVWMLRMASDWLVMELTGSSAAVGLLVALQFFPLMVLGPLGGVLADRHDKRRIVTV
                     AQGCSAVLAATLAVLTLGGWVEVWHLYAMAVLLGLVAAVEQPARQVLVGEVVGDGMLR
                     SAISTNNALNQIGGMIGPAAAGLLISAVGQGWAYATNAIVALAVCALFLSLRRSEMHP
                     GPRLARGRGQVREGVRYVLARPRLAWVMVLAGLMGAFGLNGPVVFTAFAGEWGTGAAG
                     LGLYNTAAAFGALAGAVMAARLARLRARSVVLGTGAFGAVEIVAAVMPVHVAF"
     gene            complement(987..>2001)
                     /locus_tag="VFC06_00515"
     CDS             complement(987..>2001)
                     /locus_tag="VFC06_00515"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HZL80420.1"
                     /translation="TARALFRNLELRPSVLHDVSNVDTTTQILGRPSALPFSLAPTGF
                     TRMMHHEGERAVVRVAERRGIPYALSTMGTTSIEDVAAAAPTARTWFQLYVWKNRAAG
                     EDLMARARAAGFEALVLTVDVPVSGARLRDARNGFSIPPALTARTVADAAMHPAWWIN
                     LLTTAPLTFASLQSWDGTVAELLDSLFDPTVTIADLEWLRSCWDGPLIIKGIQTVEDA
                     RRVVDAGADAIVLSNHGGRQLDRAPVPLRLVPDVREALDGSAEVWVDTGIMSGADIVA
                     ALALGADSTMVGRAYLYGLMAGGERGVDRAVEILEREVRRTMALLGVDRIDQLGPQHV
                     RLP"
BASE COUNT          300 a          730 c          744 g          227 t
ORIGIN      
        1 gaaggcgacg tgcaccggca tcaccgcggc cacgatctcg accgcaccga aggcgcccgt
       61 accgagcacg acgctccgcg cccgcagccg cgcgaggcgg gcggccatga ccgccccggc
      121 cagcgcgccg aacgcggcgg cggtgttgta gaggccgagg cccgcggcgc ccgtgcccca
      181 ctcgcccgcg aacgccgtga agacgacggg accgttgagg ccgaacgcgc ccatgagacc
      241 cgcgagcacc atgacccacg cgagccgggg tcgcgcgagc acgtaccgca cgccctcgcg
      301 gacctgtccg cgtccccgcg cgagccgcgg accggggtgc atctcggagc gtcgcagcga
      361 caggaacagc gcgcagacgg ccagcgcgac gatcgcgttg gtcgcgtacg cccagccctg
      421 cccgacggca ctgatgagca ggcccgcggc cgcggggccg atcatcccgc cgatctggtt
      481 gagggcgttg ttcgtgctga tggccgagcg cagcatgccg tcgccgacga cctcgccgac
      541 gaggacctgc cgcgcgggct gctcgaccgc ggcgacgagc ccgagcagca cagccatcgc
      601 gtacaggtgc cagacctcga cccagccgcc gagcgtgagg accgcgagcg tcgcggcgag
      661 cacggccgag cagccctgcg cgacggtcac gatccgtcgc ttgtcgtgcc ggtcggcgag
      721 cacgccgccg agcgggccga gcaccatgag tgggaagaac tggagcgcga cgagcaggcc
      781 gaccgcggcc gacgagcccg tgagctccat cacgagccag tcgcttgcca tacgcagcat
      841 ccacacgccg ctgctgccga gcagctgggc gcccgcgtac cgacggaagt tggcgatcca
      901 cagcggtgag ccggctgtcg gggcgtcggc gaccggggtg gccggcccga caggcgcctc
      961 ggtcgcgtcg tcgggcgcgg accgcatcag ggaagtcgga cgtgctgcgg acccagctgg
     1021 tcgatgcggt cgaccccgag cagggccatc gtgcggcgca cctcgcgctc gaggatctcc
     1081 acggcccggt cgacgccgcg ctcgcccccg gccatgaggc cgtacaggta ggcgcgtccg
     1141 accatcgtcg agtccgcgcc gagcgcgagg gccgcgacga tgtcagcgcc cgacatgatc
     1201 ccggtgtcga cccagacctc ggcgctaccg tcgagcgcct cgcggacgtc cgggacgaga
     1261 cgcaggggga ccggtgcccg gtcgagctgg cggccgccgt ggttcgacag cacgatcgcg
     1321 tcggccccgg cgtcgaccac gcggcgcgcg tcctcgaccg tctggatccc cttgatgatg
     1381 agcggaccgt cccagcagct gcgcagccac tcgaggtcgg cgatcgtgac cgtcgggtcg
     1441 aacagcgagt cgagcagctc ggcgaccgtc ccgtcccacg actgcaacga cgcgaacgtc
     1501 agcggcgctg tcgtcaggag gttgatccac cacgccgggt gcatcgcggc gtcggccaca
     1561 gtgcgggcgg tcagggccgg cgggatcgag aagccgttgc gcgcgtcgcg caggcgcgcg
     1621 cccgagaccg ggacgtcgac ggtcagcacg agcgcctcga accccgcggc gcgcgcgcgg
     1681 gccatgaggt cctcgcccgc ggcgcggttc ttccagacgt agagctggaa ccacgtgcgg
     1741 gcggtgggcg cggcggccgc gacgtcctcg atcgacgtcg tgcccatcgt ggacagcgcg
     1801 tacgggatac cgcggcgctc ggcgacccgc acgaccgcgc gctcaccctc gtggtgcatc
     1861 atgcgcgtga agccggtcgg cgcgagcgag aacggcagcg cggaggggcg tccgaggatc
     1921 tgggtcgtcg tgtcgacgtt gctgacgtcg tgcaggaccg aggggcgcag ctcgaggttg
     1981 cggaacagcg cgcgcgctgt c
//
LOCUS       DASRKH010000030         3619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_8695, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000030 DASRKH010000000
VERSION     DASRKH010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8695"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VFC06_00520"
     CDS             <1..267
                     /locus_tag="VFC06_00520"
                     /inference="COORDINATES: protein motif:HMM:NF014907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80421.1"
                     /translation="MVHSAIGREESRGSHQRLDGFTERDDDRFLAHSRAWFRTEGAPT
                     ISYAPVVITTSQPARRAYGAAGAAATAPPSAAAPGASAPEGASR"
     gene            264..1085
                     /locus_tag="VFC06_00525"
     CDS             264..1085
                     /locus_tag="VFC06_00525"
                     /inference="COORDINATES: protein motif:HMM:NF009051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HZL80422.1"
                     /translation="MSEECADSANCCGSCDGAGDRGSGAVLGTIRLQVQRYRPDLDTA
                     PSVQEYDVPWGQDTSVLDALTWIKDELDPSLTFRWSCRMAICGSCGFMLNGVPKLGCE
                     QFVRDYAPGPLLVEPLENFSVERDLVVDLEPFMSRLAAVKPFVVADDVPLAGPGNLQT
                     PEQMLAFHDYAMCINCLLCYSACPQVGIAADFLGPAAVTAAVRYNRDSRDHGEDLRLP
                     VLDSEAGLWACTFVGACSTACPKGVDPAAAIQQAKAAAAKAWAGEFVLPRHGAVR"
     gene            1082..1573
                     /locus_tag="VFC06_00530"
     CDS             1082..1573
                     /locus_tag="VFC06_00530"
                     /inference="COORDINATES: protein motif:HMM:NF014366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80423.1"
                     /translation="MSASTVSGRAAGTRSEGPDARPEALVVRDGAAPVSARRPYRAPT
                     SRTWFLRTREYRAYALREASSVVVGLFVFDLAVGLVCLHRGLDAWQWWVAMQTRPVNL
                     IFTALSVVMSLVHATTWFQATPKIIRIRRGRRYVADGWVVAQHYVLLLAFAAVVYVWL
                     GGL"
     gene            1573..1932
                     /gene="frdD"
                     /locus_tag="VFC06_00535"
     CDS             1573..1932
                     /gene="frdD"
                     /locus_tag="VFC06_00535"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF003977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HZL80424.1"
                     /translation="MRQWEGRTNEPLMWSLFGGGGMLAVFTMPVLVVVFGLLVPFGAF
                     GGAPGTYERMHGFLTHPLVALAVCLAFGLVLWHCCHRTYHALHDLGLHPPELVRAGIY
                     GIAILTPAVAYALSLAS"
     gene            1941..3047
                     /locus_tag="VFC06_00540"
     CDS             1941..3047
                     /locus_tag="VFC06_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80425.1"
                     /translation="MIGTLPGVRTTSAMKVRGAVRDATLAASVHNCQPWRFVVGSDSV
                     EIWLEDGPRPTVIDPLGRWAMASLGAAAANLELGLRRRLEREVRIEPWSGACVAAADG
                     RRGRGVAVPSGPAHRPVVVAAWGRDHVPATARERELHAAVGARRTARTPLYGELDPQV
                     WKSLAAAVRGRGSVDFVEAAPGNGTALAVGARAVRPDAARTAELLALTAKVDARWCDD
                     PAYLAEIARWAKVSGGRGIPTGAYGRRDASGRVAGRDFSVRLDGPPDRLVDDYFEIMP
                     QLLVVVTVGDSPGEQVLAGAAVQRAMLAATAAGLGIGVLGQLVEDADARRRASDALGL
                     GGGVVQQVLRLGRPAADDPRPARTPRRPVRRVVS"
     gene            complement(3115..>3619)
                     /locus_tag="VFC06_00545"
     CDS             complement(3115..>3619)
                     /locus_tag="VFC06_00545"
                     /inference="COORDINATES: protein motif:HMM:NF015882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HZL80426.1"
                     /translation="PERRWIAEHTNPERRTGGVESALRGADVFVGVSGPDAVRPEWLR
                     SMGSDAIVFPMANPVPEIMPEAVPPNVRILGTGRSDYPNQINNTLIFPGFFRGLLDAR
                     ARTVLPQMRVAAAHALADIVAQDGPTEDYVVPSVFDPRIVPAVSAAVQQVQHAASRPV
                     TAAAAIA"
BASE COUNT          452 a         1254 c         1346 g          567 t
ORIGIN      
        1 atggtgcact cggccatcgg acgcgaggag tcgcgggggt cgcaccagcg gctggacggc
       61 ttcaccgaac gtgacgacga ccggttcctg gcgcacagcc gggcctggtt ccgcaccgag
      121 ggtgcgccga cgatcagcta cgcgccggtc gtcatcacca cctcgcagcc cgcccggcgg
      181 gcgtacggcg cagcaggagc agcagcgacc gccccgccgt cggcggcggc gccgggtgcg
      241 tcggcaccgg aaggagcaag tcgatgagcg aggagtgcgc agattccgcg aactgctgcg
      301 gttcgtgcga cggtgccgga gaccgcggtt ccggcgcggt gctcggcacg atccgtctgc
      361 aggtccagcg ctaccgtccc gatctcgaca cggccccctc ggtgcaggag tacgacgtgc
      421 cgtggggtca ggacacctcg gtcctggacg ctctcacgtg gatcaaggac gagctggacc
      481 cgtcgctgac gttccggtgg tcgtgcagga tggcgatctg cggcagctgc ggattcatgc
      541 tcaacggtgt cccgaagctc gggtgcgagc agtttgtgcg tgactacgcc cccggcccgt
      601 tgctggtcga gcccctcgag aacttctccg tcgagcgcga cctcgtcgtc gacctggagc
      661 cgttcatgtc ccggctcgcc gcggtcaagc ccttcgtcgt ggcggacgac gtgccgctgg
      721 ccggcccggg gaacctgcag acccccgagc agatgctcgc gttccacgac tacgccatgt
      781 gcatcaactg cctgctctgc tactcggcct gcccgcaggt gggcatcgcc gcggacttcc
      841 tcgggccggc cgcggtgacg gccgcggtcc ggtacaaccg ggactcgcgc gatcacggcg
      901 aggacctgcg gctccccgtg ctcgactccg aggccgggct gtgggcgtgc accttcgtgg
      961 gtgcgtgctc cacggcctgt cccaaggggg tcgacccggc cgctgcgatc cagcaggcca
     1021 aggcggcggc tgcgaaggcc tgggccggcg agttcgtgct gccccgccac ggagcggtcc
     1081 gatgagcgcc tccacggtca gcggtcgcgc agccgggacg cgatcggaag gtcccgacgc
     1141 ccgtcccgag gcgctcgtcg tccgcgacgg cgccgcaccg gtgagcgcgc ggcgtcccta
     1201 ccgtgcaccc acgtcacgaa cgtggttcct gcggacgcgc gagtaccggg cgtatgcgct
     1261 gcgtgaggcg tcgagcgtcg tcgtcgggct gttcgtgttc gatctcgccg tcgggctcgt
     1321 ctgcctgcac cgcgggctgg acgcatggca gtggtgggtc gcgatgcaga cacggcccgt
     1381 caacctcatc ttcaccgcgc tgtcagtggt catgtcgctc gtgcacgcga cgacgtggtt
     1441 ccaggcgacg cccaagatca tccggatccg ccgcgggcgc cgctacgtcg ccgacggctg
     1501 ggtggtcgcc cagcactacg tgctgctgct cgcgttcgcc gcggtcgtct acgtctggct
     1561 cggaggcctg tgatgcgcca gtgggaaggg cggacgaacg agccgctcat gtggtcgttg
     1621 ttcggtgggg gcggcatgct cgccgtcttc acgatgccgg tactggtggt ggtgttcggg
     1681 ctgctcgtgc cgttcggtgc gttcggtggc gccccgggga cctatgagcg gatgcacggg
     1741 ttcctcacgc acccgctcgt cgcactggcg gtctgcctgg cgttcggcct cgtgctctgg
     1801 cactgctgcc accgcacgta ccacgcgctg cacgacctcg gactgcaccc gcccgagctc
     1861 gtccgagcgg gcatctacgg catcgcgatt ctgacgccgg ccgtcgccta tgcgctgtcc
     1921 ctcgcctcat gagggccccc atgatcggca ctctgccggg tgtccggacg acgtcggcga
     1981 tgaaggtgcg cggtgcggtg cgcgacgcca cgctcgccgc gtcggtgcac aactgccagc
     2041 cgtggcggtt cgtcgtgggt tccgactcgg tcgagatctg gctggaggac ggcccgcggc
     2101 ccacggtgat cgatcctctg gggcgctggg cgatggcctc gctcggtgcc gcagccgcga
     2161 acctcgagct cgggttgcgc cgacggctcg agcgtgaggt acggatcgag ccctggagcg
     2221 gcgcgtgcgt cgctgcggcg gacggtcgac gcggtcgtgg cgttgcggtg ccgtccggcc
     2281 cagcacatcg gcccgtcgtc gtggcggcct ggggtcggga ccatgtgcct gcgacggcgc
     2341 gcgagcgcga gctgcacgcc gccgtcggtg cgcgacgcac ggcgcggaca ccgctgtacg
     2401 gcgagctcga cccgcaggtc tggaagtcgc tcgcggccgc ggtgcgcgga cggggatcgg
     2461 tcgacttcgt ggaggcagcg ccgggcaacg gcacagcgct cgccgtcggc gcgcgcgctg
     2521 tgcgacccga tgcggcccgc accgccgagc tgctcgctct caccgcaaag gtggatgcgc
     2581 gctggtgcga cgacccggcg tacctggcgg agatcgcgcg atgggcgaag gtgtccggcg
     2641 gtcgcgggat acccaccggt gcctacggtc ggcgcgacgc ctcgggacgg gtggcgggcc
     2701 gggacttcag cgttcgcctg gacgggcctc ccgaccggct cgtcgatgac tacttcgaga
     2761 tcatgcctca gctgctcgtc gtcgtgacgg tcggtgactc accgggtgag caggtgctcg
     2821 ccggagcggc cgtccagcgc gcgatgctgg cggccaccgc cgcgggactg ggcatcgggg
     2881 tactcggcca gctggtggag gacgccgatg cgcgtcggcg cgcttccgat gctctcggtc
     2941 tcggcggagg ggtggtgcag caggtgctgc gcctcggacg accggccgcc gacgacccgc
     3001 gtcccgcgcg gactccacgc cgaccggtgc ggcgcgtcgt cagctgatag cagtccagac
     3061 gtcggcccgg ccccggagca cctccggagt cgggccgacg tccgtccgtg agtgtcaggc
     3121 gatcgccgca gctgcagtga cgggacgcga cgcggcgtgc tggacctgct ggaccgcggc
     3181 cgagaccgct ggcacgatcc gcgggtcgaa cacgctcggc accacgtagt cctcggtcgg
     3241 gccgtcctgc gcgacgatgt cggcgagcgc atgtgctgcg gcgacgcgca tctgcggcag
     3301 cacggtgcgc gcacgggcgt ccagcagccc gcggaagaac cccgggaaga tcaacgtgtt
     3361 gttgatctgg ttcgggtagt cgctgcgccc cgtgccgagg atccgcacgt tcgggggcac
     3421 agcctcgggc atgatctcgg ggaccgggtt cgccatcggg aacacgatgg cgtccgaacc
     3481 catcgagcgc agccactcgg ggcggaccgc gtcgggaccg ctcacgccga cgaagacgtc
     3541 ggcgccgcgc agtgcggact cgacgccgcc cgtgcggcgc tcggggttcg tgtgctcggc
     3601 gatccatcgg cgttccggg
//
LOCUS       DASRKH010000031         2017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_39253, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000031 DASRKH010000000
VERSION     DASRKH010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39253"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..679)
                     /locus_tag="VFC06_00550"
     CDS             complement(137..679)
                     /locus_tag="VFC06_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80427.1"
                     /translation="MTNDLPTYQRELLLALRLRDVPGPRIAEALAEVESHVVDTGEDA
                     YDAFGNPDEYAAELAAVHGEHSGMRGAGRVRSAGARMVISTFSAVGGMLLVLGLLAIG
                     AGDSSIPGGIAAATSAIVGAVLLGVVALVLVATVGVKGDPVRDPRTGDAMTPPFPWWA
                     VAALIAVVPVSLLIGGIAGR"
     gene            complement(676..1020)
                     /locus_tag="VFC06_00555"
     CDS             complement(676..1020)
                     /locus_tag="VFC06_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012246047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HZL80428.1"
                     /translation="MDDRQWPGEWMRGVLSLCVLSIVAEGETYGYVVAQRLQEAGLGA
                     VKGGTLYPVLTRLELDGLLVSSWREGAGGPGRKFFTVTPSGRSALAGRSTEWTIFADR
                     ASALITSRKVTQ"
     gene            1378..>2017
                     /locus_tag="VFC06_00560"
     CDS             1378..>2017
                     /locus_tag="VFC06_00560"
                     /inference="COORDINATES: protein motif:HMM:NF044385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80429.1"
                     /translation="MERVRLLFTFAGGEGHLQPMLPLARRAAAAGHTVTVTGARSLGS
                     TVRRAGLGFISTGPDVRPQRRSLQPVDLDNEYLVVGEYFAGRLAAVRAADLLALCEDL
                     RPDVIVRDEMDFGSAVTAERLGIPHASVIVIGAGCFVKPGLIAEPLSRLRADHGLPAD
                     PDLVMLTRDLVLCPFPRVFRDPADPLSPAAHYFRSEPDGRPHAGSRLLDQLAG"
BASE COUNT          314 a          693 c          688 g          322 t
ORIGIN      
        1 cacttggctg tcgttcagca ctgattgcga tggttggcgg caaagatgtg gacgacgcac
       61 gctctcagct gaacaacgga cttccgaatg gtctcgcacg tccgttgcag gcctcgaggg
      121 gtgcggcacg gggatgctat ctgcccgcga ttcctccgat gaggaggctc acgggaacga
      181 ctgcgatcag cgcggccacc gcccaccagg ggaaaggcgg cgtcatcgcg tcccccgtgc
      241 ggggatcgcg aaccgggtca cccttgaccc cgaccgtcgc gacgaggacg agtgctacga
      301 cgccaagaag gacagctccg acgatggccg aggtggccgc tgctatgcca cctgggatcg
      361 aggagtctcc ggcgccgatc gcgagcaggc cgagcaccag gagcatgccg cctaccgcgg
      421 agaacgtcga gatgaccatc ctggccccgg cggaacgcac gcgcccggcg ccacgcatgc
      481 ctgaatgttc accgtggact gcggcaagct cagcggcata ttcgtccgga ttgccgaatg
      541 cgtcgtaggc gtcttcgccg gtgtccacga catggctctc cacctccgcg agtgcttctg
      601 cgatgcgcgg gcccgggaca tcacgcagcc gtagggcgag cagaagttcg cgctgatagg
      661 tgggtaggtc gttggtcact gggtgacctt tcggctcgtg atgagcgcgg acgcacggtc
      721 cgcaaagatc gtccattcgg tggatcgccc agcgagggca gatcgtccgg acggggtgac
      781 ggtgaaaaac ttgcgcccgg ggccacctgc accttcgcgc cagctcgaga cgaggagtcc
      841 gtcgagttcg agtcgggtca ggactgggta gagagtgccg cccttgaccg cgcccagccc
      901 cgcttcctgt aggcgttggg cgaccacata gccgtaggtt tcgccctctg cgacgatgga
      961 cagaacgcag agcgagagca ctcctcgcat ccactctccg ggccattggc gatcatccat
     1021 ggctagacag tagtgctcac tagtcagtct cacaaacacg tcgggtgacg gccgcagccg
     1081 ccgtggcggc cgccgcgccc gagcagcgac ggacacttca gcccacgaga gtccgggagc
     1141 cccagggctg acttggaggc gtcgtcgtct gagctgctgg ccgaactctt cgtacgcgtg
     1201 agccgcgccc gcggggacgg cgatcaccaa cgcgcacatc gccgcctagc tgtcgtcaag
     1261 gtgcgatccg gcagagccgg acgcagggcg cgggtcccgg gtcaggcgcc gccgcgcgta
     1321 tcgtcagcga cgcccctggc gggcccatgg tgattctcgc cgtgcgatga cgcggaggtg
     1381 gagcgggtgc gtctgctctt caccttcgct ggcggcgaag gccacctgca gccgatgctt
     1441 ccgttggcga gaagggccgc ggccgcgggc cacacggtca ccgtcaccgg cgccagatct
     1501 ctcgggtcga cggtgcgccg ggccgggctg gggttcatct cgaccggacc cgacgtccga
     1561 ccccagcggc gttccttgca gccggtcgac ctggacaacg agtacctggt ggtcggggag
     1621 tacttcgccg gccggctcgc cgctgtccgg gccgcggacc tcctcgctct ctgcgaggac
     1681 ctccgccccg acgtcatcgt ccgcgacgag atggacttcg gctccgccgt gacggccgag
     1741 cggttgggga tcccgcacgc gagcgtcatc gtgatcggag cgggctgctt cgtgaagcct
     1801 gggctgatcg cggagccgtt gagccggctg cgcgccgacc acggactacc ggccgacccc
     1861 gacctggtga tgctgacccg ggacctggtg ctctgcccgt tcccgcgcgt attccgcgat
     1921 cccgccgacc cgctgtcgcc ggcggcgcac tacttccgct ccgagcccga cggtcgaccc
     1981 catgccggat cccgcctcct cgaccagctc gccggtc
//
LOCUS       DASRKH010000032         2630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_21552, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000032 DASRKH010000000
VERSION     DASRKH010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21552"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            530..1108
                     /locus_tag="VFC06_00565"
     CDS             530..1108
                     /locus_tag="VFC06_00565"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HZL80430.1"
                     /translation="MVLHRVGAYDADLAIGHLDIAAACTTPIQQVALLDAALARGFIV
                     PWELRQLSTGTTRRRDWIRRRVHAGAQSLGESYARVGLEEAGIRVVPQARLAGVGAVD
                     LLVEGRVVVEVDGHKYHSGQRQFARDRDRDRAVTLLGLTPVRFTHADAVERLDDLVEE
                     VRAVLWRVGAGSESLRTRMDSAARDSEPMWWR"
     gene            1260..>2630
                     /gene="ftsH"
                     /locus_tag="VFC06_00570"
     CDS             1260..>2630
                     /gene="ftsH"
                     /locus_tag="VFC06_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012297583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HZL80431.1"
                     /translation="MHTMRRLRWSYLFLSLAVISIAVFAFTAATQTPVTHVDTSEGFA
                     VLESGAASQVEITDGDQRVRITLGDTVPDALSAYDLKGGALIEFFYVAPQGDDVVEAV
                     KASDPALGYDSVVPQGSLLQSLLLNILPLLLFIGLIWFLLSSMQGGGNKIMQFGRSKA
                     KEVTPDTPKVKFDDVAGADEAVEELREIQEFLENPAKFQAVGAKIPKGVLLYGAPGTG
                     KTLLARAVAGEAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKNSPAIIFVDEIDA
                     VGRHRGAGMGGGHDEREQTLNQMLVEMDGFDVSTNVIMIAATNRPDILDPALLRPGRF
                     DRQIGVDAPDLKGRLKVLEVHAKGKPIAPEVDLNAIAKRTPGFTGADLANVLNEAALL
                     TARRGATAIGASELDEAIDRVIAGPQKRTRVMNDHDKAVTAYHEGGHAVVAAAMNYTD
                     PVTKVTI"
BASE COUNT          431 a          835 c          906 g          458 t
ORIGIN      
        1 ggtccacgac gcggccgatg ccgattcgcg gcgcgtcggc gtggtttgcg gttcgtagcg
       61 tcagggggac ggggacgcga ttgatgattt agcttgggtt gtcgcccgtc gttcttagga
      121 aatccgtcgt ccccaggccg actggtcctt gcgcttccgc aggcgagcca tcaacgcgca
      181 gcgcgcgaca gcgacgaacg ccgattctca gcatgtgacg atcgaacgag tacttggctt
      241 gcacaacggc tgtgcgcgct tcaaggattt ggcggaagag ggagtttctc gtcaagccct
      301 ccgcgcggcg gtgaactcgc atcggatctt gcggccgcat cgcggttgct acgcccttcc
      361 ggatgcgtcc agagaggtcg tgttgtcgac ggtgtttcgc gcgcaactga cctgtgtctc
      421 gtgggcacat cgcgtggggc tgcctctcga attgcctgcg ccgcacgttc acctcggcgt
      481 gccagcctcg cgcggtctag ggcttccgcg tcagcgacca agcaacgagg tggtgctcca
      541 tcgcgtcggc gcgtatgacg cagacctggc gatcgggcat ctggatattg cggctgcctg
      601 tactaccccc atccaacaag tcgcgctcct tgacgccgcg ctggcgcggg gattcatcgt
      661 gccgtgggag ttgcgacaac tctcgacggg gacgacgcgc cggagggatt ggatacggcg
      721 ccgagtgcac gcgggcgcac agtctcttgg cgagagctac gcacgcgtcg gtttggagga
      781 agcgggcata cgggtggtcc ctcaagctcg gctggccggt gtcggagcag tggatctcct
      841 ggtcgagggg cgcgttgtgg tggaggtcga cgggcacaag taccactcgg ggcagcgcca
      901 attcgcgcgc gaccgcgacc gggatcgggc ggtgaccctc ctggggctga caccggtgag
      961 gttcactcac gccgatgccg tcgaacggtt ggacgacctc gtcgaggagg tccgggcggt
     1021 cctttggagg gtcggcgctg gttcggagtc gctacgaacc agaatggatt ccgccgcccg
     1081 cgattcggag ccgatgtggt ggaggtagcg gcgtgtcgcg cgcgtgagcg gttcggagcg
     1141 tcgggcggcg atgcgctggt ttggattgcc ggcagtcacg cgctgggggc ctcctgtcgg
     1201 caatgccgtc ccgctgagcg cgaaacctga ccatgggccg gtcatcctcg cgtaaagtca
     1261 tgcacaccat gagaagactg cgctggtcat acctgttcct ttccctcgcg gtgatttcta
     1321 tcgccgtgtt tgccttcacc gccgccaccc aaacgccggt gacgcacgtc gacacgagtg
     1381 agggtttcgc ggtgctcgaa tcgggcgcgg cgagccaggt cgagatcacg gatggcgacc
     1441 aacgggtgcg gatcacgctg ggcgacaccg tcccggacgc gctgtccgcc tacgacctca
     1501 agggtggcgc cctcatcgag ttcttctatg tcgcgccgca gggcgacgac gtggtggagg
     1561 ccgtcaaggc ctcggacccg gcgctcggct acgactcggt ggtgccgcaa ggctcgctcc
     1621 tgcagtcgct cctgctgaac atcctgccgc tcctgctgtt cataggactg atctggttcc
     1681 tgctgagctc gatgcagggt ggcggcaaca agatcatgca gttcggccgt tcgaaggcca
     1741 aagaggtcac cccggacacc cccaaggtca agtttgacga cgtggcggga gccgacgaag
     1801 ccgtcgagga gctgcgcgag atccaggagt tcttggagaa ccccgcgaag ttccaggccg
     1861 tgggcgccaa gatccccaag ggcgtgctgc tgtacggcgc ccccggaacc ggcaagacgc
     1921 tcctcgcgcg cgccgtggcc ggcgaggcgg gcgttccatt cttctcgatc tcaggttccg
     1981 acttcgtcga gatgttcgtc ggcgtcggcg caagccgcgt gcgtgacttg ttcgagcagg
     2041 ccaagaagaa cagcccggcc atcatctttg tggacgagat cgacgcggtg ggtcgccacc
     2101 gtggcgcggg catgggcggc ggccacgacg agcgcgagca gaccctcaac cagatgctgg
     2161 tcgagatgga cggcttcgac gtcagcacca acgtcatcat gattgccgcg accaaccgcc
     2221 ccgacatcct cgacccggcg ctgttgcggc ccgggcgctt cgaccgccag atcggagtcg
     2281 acgcccccga cctcaagggc cgactcaagg tgcttgaggt gcacgccaag ggcaagccca
     2341 tcgcgccgga ggtcgacctc aatgccatcg ccaagcgcac gcccgggttc accggcgccg
     2401 accttgcgaa cgtgctcaac gaggccgccc tgctgacggc gcgacgcggt gccaccgcga
     2461 tcggtgcgag cgaattggac gaggccatcg accgcgtcat cgccggccca cagaagcgca
     2521 cgcgggtgat gaacgatcac gacaaggcgg tgaccgcgta tcacgagggc ggccacgccg
     2581 tggtggcggc ggccatgaac tacacggacc ccgtcaccaa ggtgacgatc
//
LOCUS       DASRKH010000033         2464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_12834, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000033 DASRKH010000000
VERSION     DASRKH010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12834"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VFC06_00575"
     CDS             <1..306
                     /locus_tag="VFC06_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HZL80432.1"
                     /translation="AALRDDALDALQEFGLCLERRERGRVFAALTADGWSIPDPQGNF
                     VWIEARDRTAELVAHLAAQSPAILVRPFTDEGVRITIGSPEENDAALAALAALGWRV"
     gene            379..735
                     /locus_tag="VFC06_00580"
     CDS             379..735
                     /locus_tag="VFC06_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80433.1"
                     /translation="MFTSIDDVTPIDSNEVGNRVADFLQTTGQIMLITMAAVFVLYLV
                     IAIVIAKKAGYSGWWGALAVLVPPLGVLLALLEGRPLEERKQQREQHAERRHQYCERA
                     PPTGVPGLLRNHNRDD"
     gene            complement(934..2028)
                     /gene="hisC"
                     /locus_tag="VFC06_00585"
     CDS             complement(934..2028)
                     /gene="hisC"
                     /locus_tag="VFC06_00585"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013885100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HZL80434.1"
                     /translation="MALPQPMPNISSLPSYVPGARGSASAQAPIKLSSNENPLPPLPS
                     VSEAIAAAGESVHRYPDMFAADLVNALARRQGLPPENVIVGAGSVAVLAHFLQAYAGP
                     GREVVYAWRSFEAYPILTALTGATAVTVPLAEGGRHDLDAMAAMMSERTAAVMVCSPN
                     NPTGPAVTAEEFAAFVDRVPSHVLVILDEAYVEFVSDPAIVKSNDYIGADAWAAHPNV
                     VVLRTMSKAYGLAGLRVGFAFGLAEILAPARACVTPFSVSSVAQVAALASLEAEAELL
                     ERVEGIVAERGRVLAALTADGWSIPDAQGNFVWIEARDRTAELVTHLAAQSPAILVRP
                     FIDEGVRITIGSPDENDAVLAALAALGWRV"
     gene            2065..2460
                     /locus_tag="VFC06_00590"
     CDS             2065..2460
                     /locus_tag="VFC06_00590"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HZL80435.1"
                     /translation="MRFLLKSAIAAVAVWIVTELPFDVVVTGGENGDWWHRPAVFLSI
                     GVLLVMLNAVLKPLIKVLTLPVQILTLGLFSFVIAWFMLWLTSWITDKLDFATLTVGG
                     FWKTLFAAVVIALVTGLLEAIIPGAKKKD"
BASE COUNT          442 a          776 c          815 g          431 t
ORIGIN      
        1 gccgcgctcc gcgacgatgc cctcgacgcg ctccaggagt tcggcctctg cctcgagcga
       61 cgcgagcgcg gccgagtgtt tgcggcgctc accgccgacg gttggtccat ccccgaccca
      121 caaggtaact ttgtgtggat agaggcgcgc gaccgcaccg cggagttggt cgcgcacctt
      181 gcggcccaga gccccgcaat cttggtacga cccttcaccg acgagggtgt tcgcatcacc
      241 atcggctccc ccgaagagaa cgacgccgca ctcgccgcac tcgcggcatt gggctggcgc
      301 gtgtagaaac gaatgcagtg cccgcaccac acctgatgag ggcatccgac gacagaagtc
      361 cctcagaaag gaccgctcat gtttaccagc atcgacgacg tcactccgat cgacagcaac
      421 gaggtgggca atcgcgtcgc ggacttcctg caaaccaccg ggcagatcat gctcatcacg
      481 atggctgcag tcttcgtgtt gtacctcgtc atcgccattg tgattgcgaa gaaggccggg
      541 tactccggct ggtggggcgc gctcgccgta ctggtcccgc cgctcggcgt gctgctcgcg
      601 ctgcttgagg gccggccact tgaggaacgc aaacaacagc gcgagcagca cgccgagcgg
      661 cggcaccagt actgcgagcg cgccccacca accggagtac ccggccttct tcgcaatcac
      721 aatcgcgatg actaggtaca acacaaagac tgcagccatc gtgatgagca tgagttgccc
      781 ggtggtttgc aggaagtccg cgacgcgatt gcccacctcg gtgctgtcga tcggagagac
      841 gtcgtcgata ctggtaaaca tgagcggtcc tttcataggg acttccgtcg tcgggtgcca
      901 gcaacgttag tgttgcgaac acttagttcg tttctacacg cgccagccca atgccgcgag
      961 tgcggcaagg actgcgtcat tctcgtccgg agagccgatg gtgatacgaa ctccctcgtc
     1021 gatgaagggt cgtaccaaga tagcggggct ctgggcagca aggtgcgtga ccaactccgc
     1081 ggtgcggtcg cgcgcctcta tccatacaaa gttaccctgc gcgtcgggga tggaccaacc
     1141 gtcggccgta agggccgcga gcacacggcc gcgctccgcg acgatgccct cgacgcgctc
     1201 caggagttcc gcctcggcct cgagcgacgc gagcgccgcc acttgggcca ctgacgacac
     1261 ggaaaacgga gtcacgcacg cgcgcgcggg tgccaggatc tcggcgagcc cgaaggcgaa
     1321 gccgacgcgc aatccggcca gtccgtaggc cttggacatc gtccgcaaga ccacgacgtt
     1381 ggggtgggcc gcccacgcat cggcgccgat gtagtcgttg ctcttgacga tcgccgggtc
     1441 ggacacgaac tcgacgtagg cctcgtccag aatcacgagc acgtgggagg gaacacggtc
     1501 cacaaaggcg gcgaactcct cggcggtcac tgcgggaccc gtggggttgt tgggcgagca
     1561 gaccatcact gcggccgtgc gctcggacat cattgcggcc atggcgtcca ggtcgtggcg
     1621 cccgccttcc gccaagggga cggtcaccgc cgtcgccccg gtgagggcgg tgaggatggg
     1681 gtacgcctcg aaactgcgcc acgcatagac gacctcgcgg ccgggcccgg cgtaggcctg
     1741 aaggaagtga gccagcacgg cgaccgagcc cgcgccgacg atgacgttct ctggcggcag
     1801 tccctggcgc cgcgcgagcg cgttcacgag gtcggcggcg aacatgtcgg ggtagcggtg
     1861 gaccgactct ccggcggcgg cgatcgcctc cgacaccgag ggcaaaggcg gcaacgggtt
     1921 ttcgttggac gagagcttga tcggcgcttg ggccgacgcg gagccgcgcg cgccggggac
     1981 gtagctcggc aacgaggaga tattcggcat gggctgagga agtgccattc cctcagcatg
     2041 ccagggtcgt gccattatcg gtgcatgaga ttcttgttga agtcggcgat tgccgcggtg
     2101 gccgtgtgga ttgtgacgga gcttcctttc gacgttgtcg tgaccggtgg ggagaacggc
     2161 gactggtggc accgtcctgc ggtcttcctg agcatcggcg tgctgctggt catgctcaat
     2221 gccgtcctca agccgctgat caaggtgctg acgctgccgg tacagatcct cacgctcgga
     2281 cttttctctt tcgtgattgc gtggttcatg ctgtggctca cgtcctggat caccgacaaa
     2341 ctcgacttcg cgacgctgac ggtgggcggg ttctggaaga ccttgttcgc ggccgtggtg
     2401 atcgcgctgg tcaccgggtt gcttgaggca atcatccccg gagcaaagaa gaaggactag
     2461 attt
//
LOCUS       DASRKH010000034         2291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_21524, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000034 DASRKH010000000
VERSION     DASRKH010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21524"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VFC06_00595"
     CDS             complement(<1..527)
                     /locus_tag="VFC06_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HZL80436.1"
                     /translation="MSPAKPRPIPRPQAPSPAILAAHTTHPVRIPLADDISAEAIASA
                     SAFGAVEGDQVIVIEGDTRTPVGPATGDEPITQYARAFIELAASVERFHARLVGAELG
                     PKDIDESLKGLRSSLETPTVVGNLAALRARFGIVEAEANEVAERVRAERAAAREVAAK
                     AREVIVTQAEELAAKP"
     gene            728..2059
                     /gene="hisS"
                     /locus_tag="VFC06_00600"
     CDS             728..2059
                     /gene="hisS"
                     /locus_tag="VFC06_00600"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HZL80437.1"
                     /translation="MSRIAPLSGFPEWAPAERLVELHVLDRLREVFELHGFGQIETRA
                     VEPLERLGGEAEVSKEVYVLTRRGGESAEFGLHFDLTVPFARYVEERQGDLTFPFRRY
                     QIQKVWRGERPQEGRFREFYQADIDVVARETLPSHLEAEVAVVMARALESLELPPARM
                     RMNDRRLVEGFYRGVGVTDVAGALRSVDKLGKIGPDGVAKELATRGVPETASDAILHL
                     ARIQSVDASFREAVLDLWETTPTIDDEDAQRLLGEGLEALGLLVETVNAAVPGTAIAD
                     LSIARGLDYYTGAVYETTLEGHEDLGSICSGGRYDSLVAGGGFPGVGLSIGVSRLVSR
                     LVSRGLLVATRSVPSAVLVAVMDEETRAASQAIGDRLRARGIACEVSPSAAKFGKQIQ
                     YADRRGIPYVWFPGQDADGEVKDIRSGDQIPADADAWVPPADDLKPRVVRG"
BASE COUNT          363 a          755 c          783 g          390 t
ORIGIN      
        1 ggcttggccg cgagctcttc ggcctgcgtc acgatgacct cgcgtgcttt cgcggccacc
       61 tcgcgggcgg cggcgcgctc ggcgcgcacc ctctccgcga cctcgttcgc ctcggcttcg
      121 acgatgccga aacgggcgcg cagtgcggcg aggttgccga ctaccgtcgg cgtctcgagc
      181 gatgaacgga gacccttgag cgattcgtcg atgtccttgg ggcccagttc ggcgcccaca
      241 agtcgggcgt ggaaacgctc gacggacgcg gccaactcaa tgaacgccct ggcgtattgc
      301 gtgatgggct cgtcgcctgt tgcgggtccc actggggtgc gagtgtcgcc ttcgatcacg
      361 atcacctggt caccttcgac ggcaccaaat gccgaagctg aagcgatcgc ctccgccgag
      421 atgtcatcgg cgaggggaat ccgcacgggg tgcgtcgtgt gcgccgcgag aatggcgggc
      481 gagggcgcct gagggcgagg aatgggcctg ggcttggcgg gcgacatgat gcgatgacct
      541 ttgtgaggac gacgaaacgg gcaggtgcta cctgaaggtg gcacccgtga tggtaccgcc
      601 tcaggcaatc ccactcacga tatcgaggag cggaccacac gcgccacaac cgccgcagac
      661 cgtatccctt tcgtgacccg ccctcgtctg ggcattcacg cactcccctc gatggaacaa
      721 tgggggcatg tcgcgaattg cgcccctctc aggcttcccc gaatgggccc ccgccgaacg
      781 actcgtggaa ctccacgtac tcgatcggtt gcgcgaggtt ttcgagctcc acggcttcgg
      841 ccaaatcgag acgcgcgccg tcgagccgct tgaacgcctc gggggagagg cggaggtctc
      901 gaaggaggtc tacgtcctca cgcgtcgcgg gggcgagtcc gccgagttcg ggctgcactt
      961 cgacctcacc gtgccttttg cgcgctacgt cgaggagcgc cagggtgacc tgaccttccc
     1021 gtttaggcgc tatcagatcc agaaggtgtg gagaggcgaa cggccgcaag agggtcggtt
     1081 cagagagttc tatcaggccg acattgacgt ggtggcgcgc gagacccttc cgtcgcacct
     1141 tgaggccgag gtcgccgtgg tgatggcccg cgccctcgaa agcctcgaac taccgcccgc
     1201 ccgcatgcgt atgaacgaca ggcgcttggt cgagggcttc taccgcggcg tgggggtgac
     1261 cgatgtggcc ggagccctgc gttctgtcga caagctgggc aagatcggcc ccgacggtgt
     1321 tgccaaggag cttgcgaccc ggggcgtgcc ggagacagcc tcggatgcga tcctgcacct
     1381 cgcgcggatt caatctgtag acgcttcgtt ccgcgaggcc gtgctcgacc tgtgggagac
     1441 cacgcccacg atcgacgacg aggacgcgca gcgtctgctg ggcgagggcc tcgaggctct
     1501 gggcctgctc gtcgagacag tgaacgccgc cgtgccaggc accgcgatcg cggatctgtc
     1561 gatcgcccgt ggcctcgact actacacggg cgccgtctac gagacgacgc tcgagggtca
     1621 cgaggacctc ggctcgatct gctcgggagg gcgctacgac tcgctcgtgg ctggcggggg
     1681 cttcccaggt gtcggcctgt cgattggcgt gtcccggctg gtgtcgcgac tcgtgtcgcg
     1741 cgggctgctg gtcgcgacca ggagcgttcc gagcgcagtg ctcgtggccg tgatggatga
     1801 ggagacgcgt gccgcctcgc aggccatcgg tgatcgcctc agggcccgcg gcatcgcatg
     1861 cgaagtctca ccctccgcag ccaagttcgg caagcagatt caatacgccg acaggcgcgg
     1921 cattccgtac gtgtggttcc cgggccagga tgccgacggc gaggtcaagg acatccgctc
     1981 gggcgaccag atcccggccg acgccgatgc gtgggtgccg ccagcagacg acctcaaacc
     2041 gcgcgtcgtc cgcggctaga ctggcccgtg ctccctcgcg gtgagccacc acatccgatc
     2101 gacaacgctt caatgaaagg ccgattgtgc ttcgcaccca cctcgccgga aacctccgtg
     2161 ccgccgatgc tggcgccacc gtcacgctct caggctgggt gggtcggcgt cggccgggat
     2221 ctggtcgccc gagcggatgt ccttgacctc gccgtcggca tcctggcccg ggaaccacac
     2281 gtacggaatg c
//
LOCUS       DASRKH010000035         2465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_48326, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000035 DASRKH010000000
VERSION     DASRKH010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48326"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1470)
                     /locus_tag="VFC06_00605"
     CDS             complement(<1..1470)
                     /locus_tag="VFC06_00605"
                     /inference="COORDINATES: protein motif:HMM:NF025620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HZL80438.1"
                     /translation="MRNPATLFASLTIAGALLISGCSAGTGTAAGADGATTAVGASSM
                     TGAVSELAYTHLGVPEAELLFDMADAVSVTLADGASSGGKDVSVLGDTVTLTAAGTYA
                     VTGSLTSGSLVVDAPDAVVNLVLDGVDITAADVAAINVADAGQVVVWLADGSVNTLAD
                     ASGAVVDDSAAVDDTAADSPNATLFSTADLWIAGRGALSVTAHANDGITSKDSLVIAG
                     GAVTVTAADDGLRGKDHLVVAAGTLTVTAAGDALKSDNGSVTDQPDATVGVIWIADGT
                     LDLTSGSDGADAARQVTITGGDLTIAAGDDGLHSDAVLRIDGGTIAITESVEGIEGAY
                     MYLSGGDVTVAASDDGINVSGGLEALETTADVSATVDQGMAVDPGMPADQADGMPGRP
                     GPGMAPDQGTVADPAVPVDQAPGMQGGPDSQFDTAATVDDITTLTVAAMVGSGFGGPA
                     EADNGTRFLEFSGGTYVVDTQSDGVDVNGSMTMTGGTLVV"
     gene            1654..1986
                     /locus_tag="VFC06_00610"
     CDS             1654..1986
                     /locus_tag="VFC06_00610"
                     /inference="COORDINATES: protein motif:HMM:NF014906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HZL80439.1"
                     /translation="MLFEELVGALIDAGFDYDLAHRAIHSLGSLVLGFTNELFDPDPS
                     TDEEIDGEAMLAMAEGMPHLLQLAQMGLHESDGSLSTCDTQAEFEFTVSLIVDGLEAR
                     RAALPEDG"
     gene            complement(1983..2345)
                     /locus_tag="VFC06_00615"
     CDS             complement(1983..2345)
                     /locus_tag="VFC06_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019047276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HZL80440.1"
                     /translation="MAVSQTRRARASRRRQRRVAAADNDLTAIQWDAIQEAWRACAYC
                     GATGVPMQKDCVLPISRGGRYTAENVVPACRSCNASKCNEEVTHWLRRKRLDERAFLV
                     RLYEINAGLAITAEPAAP"
BASE COUNT          362 a          859 c          859 g          385 t
ORIGIN      
        1 taccaccagc gttccgcccg tcatcgtcat ggaaccgttg acgtcgacgc cgtcgctctg
       61 ggtgtcgacc acgtacgtgc cgcccgagaa ttcgaggaac cgggtgccgt tgtctgcctc
      121 cgcggggccg ccgaagccgg agcccaccat ggccgcgaca gtcagggtcg tgatgtcgtc
      181 gaccgtggcc gcagtgtcga actggctgtc ggggccaccc tgcattcccg gcgcctggtc
      241 gacgggcacc gccgggtcgg cgacagtccc ctggtcgggc gccatgcccg gtccgggcct
      301 gccaggcatg ccgtcggcct ggtctgcggg catgccgggg tcgaccgcca tgccttggtc
      361 cacggtcgcc gatacgtcgg ccgtggtctc gagggcctcg agcccaccgg acacgttgat
      421 gccgtcgtcg gaggccgcca cggtgacgtc gccacccgac aggtacatgt atgcgccctc
      481 gatgccctcg acgctttcgg tgatcgcgat ggtgccccca tcaatgcgca gcaccgcgtc
      541 ggagtggagg ccgtcgtctc ctgcggcgat cgtgaggtcg ccgcccgtga tcgtgacctg
      601 acgcgcggca tcggcgccgt cggagcccga ggtgaggtcg agcgtgccgt ccgcgatcca
      661 gatgacgccc actgtcgcgt cgggctggtc ggtgacggac ccgttgtcgg acttgagcgc
      721 atcaccagcg gcggtcacgg tgagggtgcc cgccgcgacg accaggtggt ccttgccgcg
      781 caggccgtcg tcggcggccg tgaccgtgac ggcgccgccg gcgatcacga gcgagtcctt
      841 cgacgtgatg ccgtcgttgg cgtgcgccgt gacggacagc gcgccgcggc ccgcgatcca
      901 cagatcggcg gtggagaaga gcgtcgcgtt gggagagtcc gcagcggtgt catcaacggc
      961 cgcggaatcg tcgacaacgg cgccggacgc gtcggcgagg gtgttgacgg atccgtcggc
     1021 cagccacacg accacctgcc cggcgtcggc cacgttgatg gccgcgacgt cggcggcggt
     1081 gatgtcgacg ccgtcgagca ccaggttcac caccgcgtcg ggcgcgtcaa caacaagaga
     1141 cccggaggtc agcgagcccg tcacggcgta cgtgccggcg gcggtcagcg tcacggtgtc
     1201 gcccaagacg ctcacgtcct tgcccccgga cgatgcgccg tcggccaatg tcacggacac
     1261 ggcgtcggcc atgtcgaaga ggagctcggc ttcgggaacg ccaaggtggg tgtacgcgag
     1321 ctctgacacc gcgccggtca tcgaggacgc gccgacggcg gtcgtcgcgc cgtcagcgcc
     1381 cgccgcggtc cccgtgcccg cgctgcagcc gctgatgagc agcgctcccg cgatggtgag
     1441 gctggcgaag agggtggccg ggtttctcat cgtgaagcct ttcggtggcg ccgcgggtgc
     1501 gcgcccgggg ggtgatgtcc tacccccatc acacccggcc aacctcaaga aacccgcaga
     1561 ccggcttcac agtcccctga acgggacgtc acgtgcacgg cgccgccggc ggggctgatc
     1621 tcccagcaga ccgagtcccc cgtcaccgcg ttcatgctgt tcgaggagtt ggtcggcgcc
     1681 ctcatcgacg cgggcttcga ctacgacctc gcgcatcggg cgattcactc gctgggttcg
     1741 ctggtactgg gcttcaccaa cgaactgttc gacccggatc ccagcaccga cgaggagatc
     1801 gacggggagg ccatgctcgc gatggccgag ggcatgccgc acctgctcca gttggctcag
     1861 atgggcctcc acgagtccga cggctccctc agcacgtgcg acacacaggc cgagttcgag
     1921 ttcaccgtgt cgctgatcgt cgacggcctc gaagccaggc gggccgcact cccggaagac
     1981 ggctagggcg ccgccggctc cgcggtgatc gccagccccg cgttgatctc gtacagccgc
     2041 accaggaacg cgcgctcgtc gaggcgcttg cggcgcaacc agtgcgtcac ctcttcattg
     2101 cacttgctcg cattgcagga gcggcacgcc gggaccacgt tctcggcggt gtagcgcccg
     2161 ccgcgggaga tgggaagcac gcagtccttc tgcatcggga cgcccgtcgc gccgcaatag
     2221 gcgcatgcac gccaggcctc ctgaatggcg tcccactgga tggccgtgag gtcgttatcg
     2281 gccgcggcga cgcgtcgctg gcgacggcgt gatgcccgcg cgcgacgggt ctgactgacg
     2341 gccatgctcg tcaggctacg gccacacacg tgtcgcacgc gggtgccacg atggacacat
     2401 ggccacttcg aaggacaccg tcgcatatgt gctggagcag ctcgagcctc tcgacgtgtg
     2461 cgcat
//
LOCUS       DASRKH010000036         2674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_26552, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000036 DASRKH010000000
VERSION     DASRKH010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26552"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..1330)
                     /gene="nhaA"
                     /locus_tag="VFC06_00620"
     CDS             complement(8..1330)
                     /gene="nhaA"
                     /locus_tag="VFC06_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HZL80441.1"
                     /translation="MVRVRARRLFSRLTPSTEQTLADVLRTERAGGILLLIGTIIALV
                     WANSAWAGSYDTVKATVIGPHALHLDLALETWAKDGLLAIFFFVIGLELKREIVAGEL
                     RRPATAIVPIIAAVGGMLVPAGLYVLINATSDNGVLSGWAVPVATDIAFAIAVLSVVG
                     RWLPNALRAFLLTLAVVDDLLAIIIIAIFFSDSLAFGWLAASVACIVLFWFLIRKGIT
                     TAWVLVPLGLLAWGLMHASGVHATIAGVALGLVVPAIARPHEKHSLAEHWEHRWRPIS
                     AGMAVPVFALLAAGVVVDGAAIAAAASDPIAQGVVAGLVIGKPVGILLFTFLVATFTR
                     ASLDRGLSWWDVLGMSALAGIGFTVSLLIGDLAFAGDRVSEVKMAVLTASLLSALIGS
                     AILMWRDRFYRSVFESRSTPVPDSRLAEAASLEREFVKRGRAAAIESE"
     gene            complement(1450..2166)
                     /locus_tag="VFC06_00625"
     CDS             complement(1450..2166)
                     /locus_tag="VFC06_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZL80442.1"
                     /translation="MADLLVLTPSSDGATGVLPALGLLSHRTRVVAPEPSAMLDALDA
                     DVLVIDARNDLSEARNNCRLVQVTGVKVPLLLVLREGGMSIVTAEWGADDVVLTDASP
                     AEVEARVRLLLGKASSVDPLETSSEYKSGDLTVDVGGYTARLKGRPLDLTYKEFELLK
                     YLMQHPGRVYTRDQLLQEVWGFDYYGGSRTVDVHVRRLRAKLGAEHEQLIGTVRNVGY
                     RFDPLPIKHAAESSSRSANA"
     gene            2222..2671
                     /locus_tag="VFC06_00630"
     CDS             2222..2671
                     /locus_tag="VFC06_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80443.1"
                     /translation="MNPSTRATATFAGATVVAVSGFYGTRFLALGISALIAVAAFGWP
                     RLLRVYRRKVSTIIIAASGALSVVAVVFGRGEPYLRYAVAAAAGAVLLSLSAELFFPS
                     PPGRAITSVTGMMSGAFVAVSAAAWVAANRTPGAAGRRGKEEFGREG"
BASE COUNT          481 a          888 c          896 g          409 t
ORIGIN      
        1 gccgacgcta ctcggattcg atcgcggcgg cacggccccg cttgacgaac tctcgctcga
       61 gcgacgcggc ctccgcgagg cgggagtccg gcaccggcgt cgaccgactc tcaaagacgg
      121 acctgtagaa gcggtctcgc cacatgagga tcgccgatcc gatgagagcc gacaacaatg
      181 acgcggtcag cacagccatc ttcacttcgg agacacggtc tcccgcaaag gccagatcgc
      241 caatcaggag cgacacggtg aagccgatgc ccgccaaagc ggacatgccc agcacatccc
      301 accaactgag cccgcggtcc agagacgccc gcgtgaacgt cgcgacgagg aacgtgaaca
      361 gcaggattcc cacgggcttg ccgatcacca gtccggccac cacaccttgt gcaatggggt
      421 ccgatgccgc ggcggcgatc gccgcgccgt ccacgacgac gcccgcggcg aggagcgcaa
      481 ataccggcac ggccattccg gccgagatgg gtcgccaccg gtgctcccag tgctccgcga
      541 ggctgtgctt ctcgtgggga cgcgcgatcg cgggaacgac caatccgagc gcaacgcccg
      601 cgatcgtggc gtgcacgccc gaagcgtgca tcaaacccca ggccagcagc ccgagcggca
      661 ccaacaccca cgccgtcgtg atccctttgc ggatcaggaa ccagaacaag acgatgcacg
      721 ccaccgaggc ggccagccag ccaaacgcga ggctgtcgct gaagaagatg gcgatgatga
      781 tgatcgccag caggtcgtcg accacggcga gggtgagcag gaaggcgcgc agcgcgttgg
      841 ggagccaacg acccacgacg ctgagcacgg cgatggcgaa agcaatgtcg gtggccaccg
      901 gaacggccca tccggaaagc actccgttgt cgctcgtcgc gttgatcagg acgtagaggc
      961 cggcgggcac caacatgccg cccaccgcgg cgatgatcgg cacgatggcc gtggcggggc
     1021 ggcgcagttc gccagccacg atctcgcgct tgagctccag gccgatcaca aagaagaaga
     1081 tggcgagcag gccgtccttg gcccacgttt ccagcgccag gtcgaggtga agcgcgtggg
     1141 ggccaatcac ggtggccttg accgtgtcgt aagagccggc ccaagcggag ttggcccata
     1201 cgagagcgat gatggtgccg atgagcagca ggattccgcc cgcccgctcg gtgcgcagca
     1261 cgtccgcaag ggtctgctcc gtggacggcg tcaggcggct aaagagcctg cgggcacgga
     1321 cgcggaccat cggaacctcg tttcattgtg ggacggcacc aacaatggtg cgtcccattc
     1381 taccgagccg ccaccaccgg gttactgacc ccaacgcccg ggccactgcc cgtgcttccc
     1441 gggaggtctc tacgcgtttg cggaacgcga agaggactcc gccgcgtgct tgatggggag
     1501 cgggtcgaag cgatagccca cgttgcgaac ggtgccgatg agttgttcat gttcggcgcc
     1561 gagtttggcg cgcaagcgcc ggacgtggac gtccacggtg cgcgagccac cgtagtagtc
     1621 gaagccccac acctcctgga gcaactggtc gcgggtgtag acgcgccccg ggtgttgcat
     1681 caggtacttg aggagttcga actccttgta cgtgaggtcg agcggccgac ccttgagtcg
     1741 agccgtgtag ccgcccacgt ccaccgtcag atcaccggat ttgtactcgg aagacgtttc
     1801 caggggatcc acggacgatg ccttgcccag caggagcctc acccgggcct cgacctcggc
     1861 gggggaagcg tcggtgagca ccacgtcgtc ggcgccccat tcggcggtga cgatcgacat
     1921 tccgccctcg cgaagcacga gcagcagggg caccttgacg cccgtgacct ggacgagtcg
     1981 gcagttgttg cgcgcttcgg acaagtcgtt gcgcgcgtcg attaccaaga catcggcgtc
     2041 gagggcgtcg agcatggccg agggttcggg tgcgacgacg cgggtgcggt ggctgagcaa
     2101 gcccaaggca gggaggactc cggtcgcgcc atcactcgac ggcgtcagca caagcaggtc
     2161 ggccatggct acctccggtt gagtttacct taccgttcgc tctcgtgcga gacttacgcg
     2221 cgtgaacccc tcgacgcgtg ccacagcgac cttcgctgga gccaccgtgg tcgccgtgtc
     2281 aggcttctac ggcacccgct ttcttgcctt gggcatcagt gcgctcatcg cggtggccgc
     2341 tttcggttgg ccgcgcctcc tacgcgtgta tcggcggaag gtcagcacca tcatcatcgc
     2401 cgcgtcgggc gccctttcag tagtggccgt ggtgttcggg cgcggcgagc cgtacttgcg
     2461 ttacgcggtc gcggccgcgg ccggcgccgt actgctatcc ctctcggccg aactcttctt
     2521 tccctcgccg cccggccgcg ctatcacgtc ggtgaccggc atgatgtcgg gcgcattcgt
     2581 cgcggtctcc gcggcagcgt gggtcgccgc caaccgcacc ccgggcgcgg ccgggcggcg
     2641 agggaaagaa gagttcggcc gagagggata gcag
//
LOCUS       DASRKH010000037         2228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_53326, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000037 DASRKH010000000
VERSION     DASRKH010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53326"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VFC06_00635"
     CDS             <1..385
                     /locus_tag="VFC06_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HZL80444.1"
                     /translation="RHPVKRTIAHVDLLIVRKGEKVTVDVPVHVTGEPFPGTVAVTEH
                     TTLSVLAEATHIPEWLQVSVEGLNEGERITAGDVTLPAGSELMTDPDSTVVTISVPRG
                     AISEEEEEAAEEANEVAADAEADSE"
     gene            496..1083
                     /gene="pth"
                     /locus_tag="VFC06_00640"
     CDS             496..1083
                     /gene="pth"
                     /locus_tag="VFC06_00640"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010534311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HZL80445.1"
                     /translation="MTALVVGLGNPGPEYAQTRHNVGQMVADELARRWSGSFSALGRT
                     HAQVASIRIGGVGPSSVSAALAKPMSYMNLSGGPVSALAKYYGVAPADVMVIHDDLDI
                     PFGAIRLKRGGGSGGHNGLKDVSKALDLPDYVRVRVGIGRPPGRQAPADFVLKPFSAT
                     ERKELDLVVALAADAVEDVLVKGLAEAQQRFHASD"
     gene            1246..1998
                     /locus_tag="VFC06_00645"
     CDS             1246..1998
                     /locus_tag="VFC06_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="HZL80446.1"
                     /translation="MTSGRTEWTIPQIGDRSFGAVLFDLDGVLTPTADVHMAAWRRMF
                     TDYFTARGITPEYTDDDYFAHVDGRPRYEGVQECLASRGVELPYGSPEDPPGDQTVCA
                     LGNSKNDAFNAVLREDGVDPFPGSVALLDALQAQGVPVAVVSSSRNAADVLKVAGLAH
                     RFDVVVDGKVAAAEGLPGKPAPDTYQYAARLLGVPDSASVVVEDAISGVQAGAAGDFA
                     LVIGVDRGAGADDLVRAGADIVVGDLAELVTP"
     gene            1995..>2228
                     /locus_tag="VFC06_00650"
     CDS             1995..>2228
                     /locus_tag="VFC06_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80447.1"
                     /translation="MKAANKPQSDTGLGDYVDRLRFPADPWRLLETSFDGNDIGTNET
                     LFSVGNGYLGFRGNVEEGRDFHSHGTFINGFHET"
BASE COUNT          369 a          729 c          752 g          378 t
ORIGIN      
        1 gcgtcacccc gtcaagcgca ccatcgcgca cgtcgacctg ctgatcgtcc gtaagggcga
       61 gaaggtcacc gtcgacgtcc cggttcacgt gacgggcgag ccgttccccg gaaccgtcgc
      121 cgtgaccgag cacaccacgc tgtcggtgct ggccgaggca acccacatcc ccgagtggct
      181 ccaggtgtcg gtcgagggac ttaacgaggg cgagaggatc actgccggcg acgtgacgct
      241 gcccgccggc tccgaactga tgaccgaccc cgattcgacc gttgtgacga tctccgtgcc
      301 ccgcggcgcc atctccgagg aagaggaaga agccgccgag gaggccaacg aggtggccgc
      361 cgacgccgag gccgattccg agtaggactt ttcagcatga gcgcccggct agcccctctg
      421 tggcgagccg ggcgtcgtgc tttccgctca ttcgtggcat cgtctggacg gaaggcatcg
      481 gatgaggagg aaaccgtgac agcgctggta gtcggcctgg gcaaccccgg gcccgagtat
      541 gcgcagactc gccacaacgt cggccagatg gtggccgacg agttggcgcg tcggtggtcg
      601 ggatccttct ctgcgctggg gcgcacgcac gctcaggtgg ccagcatccg catcggcggc
      661 gtcgggccgt cgtccgtgtc ggcggcgctc gccaaaccca tgtcctacat gaatctctcc
      721 ggcggaccgg tcagcgcctt ggccaagtac tacggcgttg cgccggccga cgtcatggtg
      781 attcacgacg atctcgacat cccgtttggt gcgattcggc tcaagcgtgg cggcggctcc
      841 ggcgggcaca acggactgaa ggacgtgtcg aaggctctcg acctcccgga ctacgtccgg
      901 gtgcgggtgg gcatcggtcg cccgcccggc cgtcaggccc ccgccgactt cgtcctcaag
      961 cccttcagcg ccaccgagcg caaggagttg gacctggtgg tcgcgcttgc cgctgacgca
     1021 gtcgaggacg tgctggtgaa gggtctggcg gaagcgcaac aacggttcca cgcctcggac
     1081 tagggacgcc cgccgcgaga ggccgacgtg tcgcggtgtc gaaggcgtcc gcactggaca
     1141 atgccccccg aggtgccaag tacgatcgaa gttgtcggcc ccccatgttg ggggctcggc
     1201 agtccgcatg cctcgccgca cccagaagcc aaaggaagat tccccttgac ctcaggccgc
     1261 actgaatgga ccattccgca gatcggcgac cgcagctttg gcgctgtgct ctttgatctt
     1321 gacggggtgc tcactcccac cgcagacgtg cacatggccg catggcggcg gatgttcacc
     1381 gattacttca ctgcgcgtgg catcacgccc gaatacacgg acgacgatta cttcgcccac
     1441 gttgacggta ggccgcgcta cgaaggcgtg caggagtgtc ttgcatcacg aggcgtggaa
     1501 cttccctacg gttcgccaga ggatcctcct ggcgaccaga cggtgtgcgc gttgggcaac
     1561 tccaagaacg acgctttcaa cgcggtgctc cgcgaggacg gcgtcgaccc ctttcccggg
     1621 tctgtggcgt tgctggatgc cttgcaggcc cagggcgttc cggtggccgt ggtgtcgagt
     1681 tcgcgcaatg cggcagacgt gctcaaggtt gcgggtctgg cccaccgttt tgacgtagtg
     1741 gtggacggca aggtcgccgc tgccgagggc ctgccgggca agcccgcgcc cgacacgtac
     1801 cagtacgccg cgaggctgct gggtgtgccc gactcagcgt ccgtcgtggt ggaggatgcc
     1861 atctcgggcg ttcaggccgg agccgccgga gactttgcct tggtgattgg cgtggatagg
     1921 ggagcgggcg cagacgacct cgttcgggct ggcgccgaca tcgtggtggg agacctcgcg
     1981 gagctggtca cgccatgaag gccgcaaaca agccgcaatc cgacaccggg ctgggcgact
     2041 acgtggatcg gctgcgattc cccgccgacc cgtggcggct cctggaaacc agctttgacg
     2101 gcaacgacat cggcaccaac gagaccctgt tctcggtggg caacggctac ctcggctttc
     2161 gcggcaacgt cgaggagggc cgcgacttcc actctcacgg caccttcatc aacggctttc
     2221 acgagaca
//
LOCUS       DASRKH010000038         2520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_17056, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000038 DASRKH010000000
VERSION     DASRKH010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17056"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(252..1708)
                     /locus_tag="VFC06_00655"
     CDS             complement(join(252..1351,1351..1708))
                     /locus_tag="VFC06_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611176.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HZL80448.1"
                     /translation="MTGPGSTGEAVGMEDVPDPQVPERARRRTYTAKYKRDILTEYDS
                     LNRQGRGALLRREKLYTSLVSSWRDQRDKGVLVALARPAGAPPASTSEKDAARLRKEN
                     LRLSGELDTARQVIAIQGKTLGSVGSALDRQQREEHREMIDATITELAPLVGTRGACL
                     AVGRSRATHYRQNPRSPRPPSSPRPRPRPAMQPRALTEPERAAVLEVLRSSRFVDKAP
                     AEIQAILLDEGTYLCSVATMYRLLRAHGETGERRAQAEHPARVKPELIAPSPNRVWSW
                     DISKLKGPAKWSYFHLYTIIDIYSRYVVGWMVATRESAELAERLLLDTIEKQNIRRDQ
                     LTIHSDNGSSMASKPVAFLLADLGVTKSHSRPHTSNDNPFSESHFKILKYRPEFPDRF
                     YSLAEARSFCTEFYDWYNGEHRHSGIGMHTPFNVHHGRAPAIQHARARVLTTAYTKHP
                     ERFVRQRPQPPTFPTTSWINEPEKERTNSMTTKRN"
     gene            complement(1845..2381)
                     /locus_tag="VFC06_00660"
     CDS             complement(1845..2381)
                     /locus_tag="VFC06_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80449.1"
                     /translation="MTVPVFVDVDGVINAMPDKADDLDHWPYDSWQRDFIYMPAFAEA
                     LPITWSTAAIDGLRLIEQTPGVEMLWCTTWKHRAPKLLSPIIGLGAQWEYKDDLITNA
                     PRWFDWWKATRVYEAVQEHGRAVWIDDDIDAWTRMLRMGGRLDELDWIDDRVLTVCPD
                     TSKGLTAHELTTIEEFLR"
BASE COUNT          462 a          778 c          780 g          500 t
ORIGIN      
        1 cgaactcggc ttccttgtga ggcggatatc aaggcccaca acagcactcg cgcgcaagtt
       61 aaagggcgaa ctggtccaga cgctacgtaa gaaaatcacg aagtacgtcc atgccagacg
      121 tattctcagg caattgattg actgacttcc gcgaggacgc cctgtggcac ttgatcgatt
      181 ctctcgagac ccagatgttt gagtgtctgg ccaacccaac gctcgcggcg gaagatgtca
      241 acctatatga gtcagtttct tttggtggtc attgagttcg ttctttcctt ctcgggttcg
      301 ttgatccagg acgtcgtcgg gaacgttggt ggctgcgggc gctgccgaac gaagcgttca
      361 gggtgcttgg tgtaggcggt cgtgagaacg cgggcgcgag cgtgctggat ggcgggtgca
      421 cggccgtggt gaacgttgaa cggggtgtgc attccgatgc cggaatggcg atgttcgccg
      481 ttgtaccagt cgtaaaattc ggtgcagaat gagcgtgcct cggcgaggct gtagaaccgg
      541 tcggggaact cgggccggta cttcaaaatc ttgaagtgcg actcgctgaa cggattgtca
      601 ttactcgtat gcggccttga gtgcgacttg gtgacgccaa gatcggcaag cagaaacgcg
      661 acgggtttcg acgccatcga ggacccgttg tcggagtgga tcgtcagctg atcgcggcga
      721 atgttctgct tctcgatcgt gtcgagcagg agccgctcgg ccagctcggc ggactcccgg
      781 gtcgcgacca tccacccgac gacataccgg gagtaaatgt cgataatcgt atacaggtgg
      841 aagtacgacc acttggccgg acccttgagc ttcgagatgt cccacgacca cacccgattc
      901 gggctcggcg cgatgagttc gggcttgacg cgtgccgggt gctcggcctg agcacgacgc
      961 tccccggtct caccgtgggc gcggagcagg cgatacatcg tcgccacgga gcacaggtag
     1021 gtgccctcgt cgaggaggat cgcctggatc tccgctggcg ccttatccac gaagcggctc
     1081 gaccgcagca cctccagaac agcggcgcgc tcaggctcag tcagcgcgcg cggctgcatc
     1141 gccggccgtg gccggggccg cggcgaagac ggcggccgtg gagagcgggg gttctgacgg
     1201 tagtgcgtcg ccctcgaccg acccacggcc aggcacgcac cccgcgtgcc cacgagaggg
     1261 gcgagttcgg tgatggtggc gtcgatcatt tctcggtgtt cttctcgctg ctgtcggtcg
     1321 agagctgatc caacagagcc gagagttttc cctggatagc aatcacctgc ctcgccgtat
     1381 ccaactcgcc cgacaaccgc agattctctt tccgcagccg ggccgcgtcc ttctccgacg
     1441 tgctggctgg cggagctccc gccggccgtg ccaacgcgac cagcaccccc ttgtctcgct
     1501 ggtcacgcca cgagctgacc aaggaggtat acagcttttc ccggcggagc agcgctccgc
     1561 ggccctgcct gttcaggctg tcgtactcgg tcagaatgtc gcgtttgtac ttggccgtgt
     1621 aggtccgccg ccgagcacgc tcgggaacct ggggatcggg aacatcttcc atccctacag
     1681 cctccccggt cgagccgggc cctgtcatta ctgcgtccgt caaggcgaac tccttcccca
     1741 ccctctacga cactattcgg gacgatcccg atgccttaca caaggttgac agagagggtt
     1801 gagccagaac tccgttccgt gcacgtctcc cagcaacccg accttcatcg caggaactcc
     1861 tcgatggtcg tcagttcatg tgcagtgagc cctttcgaag tgtccgggca cacggtgagg
     1921 acccggtcgt caatccagtc gagttcgtcg aggcggccgc ccatgcgaag catccgtgtc
     1981 caggcgtcga tatcgtcatc gatccagacg gcacgcccat gctcctggac ggcctcgtag
     2041 acgcgggtgg ctttccacca gtcgaaccaa cgcggtgcat tggtgatgag gtcgtccttg
     2101 tactcccact gtgcaccgag cccgataatc ggcgaaagca gtttgggggc gcggtgcttc
     2161 catgtggtgc accacagcat ctccacacca ggggtttgtt cgattaggcg caggccgtcg
     2221 attgcggccg ttgaccaggt aatcggcagt gcctcagcga acgcgggcat gtatatgaag
     2281 tctcgctgcc acgagtcgta cggccagtgg tccaagtcgt cggccttgtc gggcatcgcg
     2341 ttgatgacac catccacatc gacgaacacg gggacagtca tagcgatccc agccgagaac
     2401 cccgcggcgg tgccagcgtc tcaatccagg tcatcgttcg cctgcccatc gctccggcac
     2461 gactggcatg ctgcactgcc acactcatca ccacgacaat tgagcgaaca agtccggagc
//
LOCUS       DASRKH010000039         2286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_66332, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000039 DASRKH010000000
VERSION     DASRKH010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66332"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1199
                     /locus_tag="VFC06_00665"
     CDS             <1..1199
                     /locus_tag="VFC06_00665"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HZL80450.1"
                     /translation="LSALAELADRAAEVGVERFVLDDGWFRHRRDDHAGLGDWYVDEE
                     VWPDGLGPLVEHVRAAGMEFGLWVEPEMINVDSDLARKHPDWILSTGNRLPPEARFQQ
                     VLDLANPAAYDHILERLDSLVREYTIDYLKWDHNRDLVEAGSTAHGRPGVHEQTLAVY
                     RLIDELKARHPQLEIESCSSGGARADLGILERTDRIWASDCIDALERQQIQRWTGLLL
                     PPELIGAHVGSPVSHSTGRAHTLTFRAGTALFGHLGVEWDLREATGSELAELAGWIAL
                     YKATRGLLHTGRVVHVDHPDPALLVHGVVADDGSAGVYALVAIATSAWYPPGRVRLTG
                     LDPDADYRLAPLEPATAVPTLGGHLPPPWWATGVVLTGRVLADVGVQAPNLLPDSLVL
                     LAATRL"
     gene            1253..2203
                     /locus_tag="VFC06_00670"
     CDS             1253..2203
                     /locus_tag="VFC06_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HZL80451.1"
                     /translation="MTTTPAPARVRGRTRPTPGTATRPKSRRARNDLKAALIFVLPAT
                     IGFAVFYVWPALRGIYYSFTDFSVLSPETWVGTANYQALLKDDLVWNALKVTLEYVVI
                     NIGAQTVLALTIAVLMHRFTKSMAVRGALLLPYLVANVVVALTWFWMMDYQIGIVNQM
                     LEWVGIDRVAFFGDSAWAIPTIAMVNVWRHVGYTALLIFAGLQTIPDQLYEAAALDGS
                     GEVRSFFKITIPLLRPVLAMVLVITVTGSFQLFDTVAVTTAGGPINATRVIYYYIYDL
                     AFTRFQFGYASAISVVLFAILAAVAFAQIKLLRAGESDLS"
BASE COUNT          338 a          852 c          712 g          384 t
ORIGIN      
        1 atctcagcgc cctcgcggaa ctggccgacc gcgccgcgga ggtcggcgtc gagcgcttcg
       61 tgctcgacga cggctggttc cgccaccgtc gcgacgacca cgccgggctc ggggactggt
      121 acgtcgacga ggaggtctgg ccggacggtc ttggcccgct cgtcgagcac gtccgcgcgg
      181 ctggcatgga gttcgggctg tgggtcgagc cggagatgat caacgtcgac tccgaccttg
      241 ctcgcaagca ccccgactgg atcctgtcca cggggaaccg cctgccaccc gaggcgcgct
      301 tccagcaggt gctcgacctc gcgaatccgg ccgcgtacga ccacatcctc gagcggctcg
      361 actcgctggt gcgcgagtac acgatcgact acctgaagtg ggaccacaac cgcgacctcg
      421 tcgaggcggg cagcacggcg cacggtcggc ccggcgtcca cgagcagacg ctggcggtct
      481 acaggttgat cgacgagctg aaggcgcgcc acccgcagct ggagatcgag tcgtgctcct
      541 cgggcggtgc cagggccgac ctcggcatcc tggaacgcac ggaccgcatc tgggcgagcg
      601 actgcatcga cgccctcgag cgtcagcaga tccagcgctg gaccgggttg ctgctgccac
      661 ccgagctcat cggcgcgcat gtgggctccc cggtgtccca ctcgacgggc cgcgcccaca
      721 ccctcacgtt ccgagccggg accgcgctct tcggccacct cggtgtcgag tgggacctgc
      781 gggaggcgac gggctccgag ctcgccgagc tcgcggggtg gatcgcgctc tacaaggcca
      841 cgcgcgggtt gctccacacc gggcgcgtcg tccatgtcga ccatccggac cccgccctgc
      901 tggtgcacgg cgtcgttgcc gacgacggct ccgctggggt ctacgcgctg gtcgcgatcg
      961 cgacttcggc ctggtacccg cccggtcgcg tccggctgac gggcctcgac cccgacgccg
     1021 actaccgtct cgccccgctc gagccggcaa ccgccgtccc gacgctcggt gggcatctcc
     1081 ccccaccgtg gtgggcgacc ggcgtcgtcc tgaccggacg cgttctggcc gatgtcggag
     1141 tgcaggcacc gaacctgctg cccgactctc tcgtgctcct ggccgccacc cggctctgag
     1201 tcgctcgtct tcttccccga ttccccaccg caacgaagcg ctcggaggac tcatgaccac
     1261 cacaccagca ccggcccgcg tgcgaggtcg aacacgtccg acgcccggca ctgccactcg
     1321 accgaagtcg agacgagccc ggaacgacct caaggccgcc ctgatcttcg tccttccggc
     1381 caccatcggg ttcgccgtct tctacgtctg gccggccctg cgcggcatct actacagctt
     1441 caccgatttc agcgtgctct ctccggagac gtgggtcggg acggccaact accaggcgct
     1501 gctcaaggac gacctcgtct ggaacgcact caaggtgacc ctggagtacg tcgtcatcaa
     1561 catcggcgcc cagacggtgc tcgcgctgac catcgcggtg ctgatgcacc ggttcacgaa
     1621 gtcgatggcc gttcgcggcg cactcctgct gccctacctc gtggccaacg tcgtcgtcgc
     1681 actcacctgg ttctggatga tggattacca gatcggcatc gtcaatcaga tgctcgagtg
     1741 ggtcgggatc gaccgggtcg cgttcttcgg cgattccgcc tgggcgatcc ccaccatcgc
     1801 catggtcaac gtctggcgac acgtcggcta caccgcgctg ctcatcttcg cggggcttca
     1861 gaccatcccg gaccagctct acgaggccgc cgcgctcgac ggctcgggcg aggtccgatc
     1921 cttcttcaag atcaccatcc cactgctgcg tccggtgctc gcgatggtgc ttgtcatcac
     1981 ggtcaccggg tcgttccagc tcttcgacac cgtggccgtc accacggccg gcggtccgat
     2041 caacgccacc cgggtcatct actactacat ctacgacctg gccttcaccc ggttccagtt
     2101 cgggtacgcc tccgcgatct cagtcgtcct gttcgccatc ctcgccgcgg tggccttcgc
     2161 ccagatcaag ctgctgcgcg ccggagagtc ggacctgtca tgaagggcca aggcacgtcg
     2221 atgaccgcca ccgccatccc cgagacgctc ccacaggccg ctcgaccgaa cgtccggcgg
     2281 cagcgg
//
LOCUS       DASRKH010000040         2202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_57730, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000040 DASRKH010000000
VERSION     DASRKH010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57730"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VFC06_00675"
     CDS             <1..254
                     /locus_tag="VFC06_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80452.1"
                     /translation="RKPVTAPEPLGSLSQGDDLAGPGGTECRSCRGTLLTQLRMVLGD
                     GTPVVFVSCHQCEERSWFALDGTGVELTREDVLERSAKR"
     gene            318..1814
                     /locus_tag="VFC06_00680"
     CDS             318..1814
                     /locus_tag="VFC06_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HZL80453.1"
                     /translation="MPTNDAVTAAAGPAPDLSGLIKAYDVRGVVPDQLNPVVARAIGA
                     AFADVVAVPEASAGQRPQAVIARDMRPSGPELVAAFADGLIARGVDVTLIGLASTDGL
                     YYASGLLDIPGAMFTASHNPAQYNGIKMCRAGARPVGQDSGLAAVRDLASQYLAAGVP
                     ALAADGVRGSISERDLLADYAGFLRSLVDLSGIRPLKVVVDAGNGMGGFTAPAVLGTG
                     AGLPQLPLDVVPLYFELDGTFPNHEANPLEPANLLDLQAAVVAHGADIGLAFDGDADR
                     CFVVDEHGDPVSPSAITALVGLREVAREQAAGRTPTIIHNLITSRVVPDLARAAGATV
                     VRTRVGHSFIKAEMAEHDAVFGGEHSAHYYFRDFFFADTGMLAALHVLAALGGQSHPL
                     SALAEQYQPYSASGEINSRVADVPAARARVVRAYVTDQGAGPVAVDELDGLTVSHWDT
                     SPQWWFNLRASNTEPLLRLNVEAADEDIMVKVRDDVLALVRAGLTEGI"
     gene            1816..2034
                     /locus_tag="VFC06_00685"
     CDS             1816..2034
                     /locus_tag="VFC06_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80454.1"
                     /translation="MTSNDAREGTPGLEPWLRDILRCPVTGAELVDGVGPDGSPELHS
                     TDPDNPLAYPVRDGIPVLLADDARSLAR"
BASE COUNT          286 a          823 c          778 g          315 t
ORIGIN      
        1 cgcgcaagcc ggtcacggcg ccggagccgc tgggttccct cagccagggc gacgacctgg
       61 ccggcccggg tgggacggaa tgccggtcgt gccgcgggac gctgctcacg cagctgcgga
      121 tggtgctcgg tgacggcacg ccggtcgtgt tcgtgtcgtg ccaccagtgc gaggagcggt
      181 cgtggttcgc gctcgacggc acgggtgtcg agctgacccg cgaggacgtc ctcgagcgca
      241 gcgccaagcg gtgaccagcg gcgtgcggtc gggcccgcga ggcccggcgg tggcgtcggg
      301 gccagtaaag ttgccgcgtg cccacgaatg acgcagtgac cgctgccgcc gggccggcgc
      361 ctgacctgtc gggcctcatc aaggcctacg acgtgcgcgg cgtcgtaccc gaccagctca
      421 acccggtggt cgcgcgggcg atcggcgcag ccttcgccga cgtcgtcgcc gtccctgagg
      481 catcggcggg tcagcgtccg caggccgtca tcgcccgcga catgcgaccg tcgggccccg
      541 agctcgtcgc ggcgttcgcg gatggcctga tcgcgcgtgg ggtcgacgtg acgctcatcg
      601 gtctggcgtc gacggacggc ctgtactacg cgtcgggcct gctcgacatc ccgggcgcga
      661 tgttcaccgc gagccacaac cccgcccagt acaacggcat caagatgtgc cgcgccggtg
      721 cgcgcccggt cggtcaggac tcgggccttg cggccgtccg cgacctcgcg tcgcagtacc
      781 tcgccgcggg cgtccccgca ctggccgccg acggcgtccg gggcagcatc tcggagcgcg
      841 acctgctcgc ggactacgcg ggcttcctgc gttcgctcgt ggacctgtcg ggcatccgcc
      901 cgctcaaggt cgtcgtcgat gcgggcaacg gcatgggcgg gttcaccgcg ccggccgtgc
      961 tcggcaccgg cgcaggcctg ccgcagctgc ccctcgacgt cgtcccgctg tacttcgagc
     1021 tcgacggcac cttccccaac cacgaggcga acccgctcga acccgcgaac ctgctcgacc
     1081 tgcaggccgc cgtcgtcgcg cacggcgcgg acatcgggct cgcgttcgac ggcgacgcgg
     1141 accgctgctt cgtggtcgac gagcacggcg acccggtcag cccgtcggcg atcaccgcgc
     1201 tcgtcgggtt gcgcgaggtc gcgcgcgagc aggccgcggg gcgcacgccg acgatcatcc
     1261 acaacctcat cacctcccgc gtcgtcccgg acctggcgcg cgctgccggc gccacggtcg
     1321 tgcgcacgcg ggtcgggcac tcgttcatca aggccgagat ggctgagcac gacgccgtgt
     1381 tcggtggtga gcacagcgcg cactactact tccgcgactt cttcttcgct gacaccggca
     1441 tgctcgccgc gctgcatgtg ctcgcggcgc tcggcggcca gtcgcacccg ctctcggccc
     1501 ttgccgagca gtaccagccg tactccgcga gcggcgagat caactcgcgc gtcgcggacg
     1561 tcccggcggc gcgggcacgc gtcgtgcggg cgtacgtcac ggaccagggt gccgggccgg
     1621 tcgcggtcga cgagctcgac gggctgaccg tctcgcactg ggacacctcg ccccagtggt
     1681 ggttcaacct ccgggcgtcc aacaccgagc cgctgctgcg gctcaacgtc gaggccgccg
     1741 acgaggacat catggtcaag gtgcgcgacg acgtcctggc gctggtgcgc gcgggcctga
     1801 ccgaagggat ctgacatgac atcgaacgac gctcgcgagg gcaccccggg tctcgagccg
     1861 tggctgcgcg acatcctgcg ctgcccggtg acgggtgccg agctggtcga cggcgtgggc
     1921 cccgacggct cgcccgagct gcactcgacc gaccccgaca acccgctggc ctacccggtg
     1981 cgcgacggca tcccggtgct actcgccgac gacgcgcgct cgctcgcccg ctgagctcgc
     2041 tgagcccgct gagcctgcta gcccgaaacc ggacctcgcg cccgactacc cgtcggcgct
     2101 gacaggagta cctcatggcc cacgacggcg gtagcagggc gatcatcgcg gcgctcggtg
     2161 cgaacctcgg catcgcggcg acgaagctgg tcgccttcct cc
//
LOCUS       DASRKH010000041         3940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_21451, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000041 DASRKH010000000
VERSION     DASRKH010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21451"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1797
                     /gene="glmS"
                     /locus_tag="VFC06_00690"
     CDS             <1..1797
                     /gene="glmS"
                     /locus_tag="VFC06_00690"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HZL80455.1"
                     /translation="GLARLEYRGYDSAGLAVVTSDKEHLAVAKKAGKLVNLAKEVAEH
                     PLEHGTAAIGHTRWATHGPPTDTNAHPHLAADGRIAIIHNGIIENYGVLRAEMLAEGV
                     TFHSDTDTEVAGHLLAHFVRDGLSLTDAMRAVARRLEGAFTLLAVDAAEPHVVVGTRR
                     NSPLVAGIGEGENFLGSDVVAFIEHTRIALELGQDEVVEITPDAVRVTDLAGNPVHRE
                     PYEVTWDASAAQKGGFATFMDKEIHDQPAAVADTLRGRLNEQGRLKLDEGLIAESVLS
                     SIDKIIVIACGTAAYAGMVARYAIEHWTRIPVEVELAHEFRYRDPVLSIRTLVVAMSQ
                     SGETMDTIMALRHAQQQGSPVVAIVNVQGSTIAREADAVLYTRAGPEIAVASTKAFLA
                     QIAAAYLLGIYLAELRGNKFPDEIAALLSELDAMPAKIQRVLDTSEPLRELARSMVDE
                     RTVLFLGRHVGYPVALEGALKLKELAYIHAEGFAAGELKHGPIALVEPGQPVFIVLPS
                     PRGREVLHSKVISNIQEVRARGARTIVIAEEGDTAAAEHADHLITVPVTPTLLMPLVT
                     VVPLQIFAHELASAKGFDVDQPRNLAKSVTVE"
     gene            1800..2156
                     /locus_tag="VFC06_00695"
     CDS             1800..2156
                     /locus_tag="VFC06_00695"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018154990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HZL80456.1"
                     /translation="MAVVGVGIDVVELERFDRAVERTPRFAEKVFTPSERQLGRHSMA
                     ARWAAKEALAKALGAPGGLSWQDAEVVRDPSGQPRFVISGTVAARAAELGIATLHVSL
                     SHDAGIASAVVIAEAS"
     gene            2153..3259
                     /gene="alr"
                     /locus_tag="VFC06_00700"
     CDS             2153..3259
                     /gene="alr"
                     /locus_tag="VFC06_00700"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HZL80457.1"
                     /translation="MSSLKVVVDSAAITHNVRTLGKMTEAQVMAVVKADGYGHGLIEA
                     AAAARAGGAAWLGVAQPHEALALRAAGDTGRMLTWLYGPEFPAEELMAADVDLSVNSP
                     EVLGRIVTASRDGSPSPRIHIAVDTGLGREGVTIANLPGLLELAKAVEREGLVRVVGM
                     WSHLAWADAPGHPTIDLQAQHFRQALVMAQDASIALEVRHLANSACTLTRPDLHFDLV
                     RPGIAIYGLPPIEDPEGQNFGLRPAMSVTSQIILVKDVLAGQGVSYGHEYVTTYPTTL
                     GLVSAGYADGVFRAAGSVAEVAVKGRRYLIAGRVCMDQFVIDLGPATEVCVGDEVTLI
                     GPGGPSARDWATAVGTIDYEIVCRFGGLRAKESA"
     gene            3256..3753
                     /gene="tsaE"
                     /locus_tag="VFC06_00705"
     CDS             3256..3753
                     /gene="tsaE"
                     /locus_tag="VFC06_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HZL80458.1"
                     /translation="MKYRVPNGEAMRSLGRELAGVFTAGDLVILSGGLGAGKTTLVQG
                     IGAGLDVRGQVASPTFIISRVHEPTAGGPALVHVDAYRLGSLVELDHLDLDASADEAV
                     TVVEWGEGKAEALAEGHALITIHRPRGALAEGEDAAAGARTVTITTTGNAWNGRSWPA
                     EGWTA"
     gene            3750..>3940
                     /locus_tag="VFC06_00710"
     CDS             3750..>3940
                     /locus_tag="VFC06_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HZL80459.1"
                     /translation="MIVSLDTSSAISVVIADAGATEALSSRSVFAPRGHAEQLSDLVR
                     QAMEEAGVARPDVTGIVVGT"
BASE COUNT          643 a         1282 c         1367 g          648 t
ORIGIN      
        1 ggcttggcgc gtctcgagta ccgaggctac gactccgcgg gcctggccgt ggtgacgagc
       61 gacaaggagc acctcgccgt ggccaagaag gccggcaagc tggtgaacct ggccaaggag
      121 gtcgccgaac acccgcttga gcacggcacg gcggccatcg ggcacacgcg ctgggcgacg
      181 catggaccgc ccacggacac caacgctcac ccgcacctgg ccgccgacgg ccggattgcc
      241 atcatccaca acgggatcat cgagaactac ggtgtgctcc gcgcggagat gctcgccgaa
      301 ggcgtcacct tccactccga caccgacacc gaggtggctg ggcacctgtt ggcgcacttt
      361 gtgcgagacg gcttgagcct gaccgatgcc atgcgggcag tcgcccgacg tcttgagggt
      421 gctttcacac tccttgccgt ggacgcggca gaaccccacg tggtcgtcgg aacgcgccgc
      481 aactcccccc tggtcgcggg catcggcgag ggcgagaact tcctgggatc cgatgtggtg
      541 gccttcattg agcacacccg gatcgctctt gagcttggcc aagacgaggt ggtagagatc
      601 acgccggacg cagtgcgcgt cactgacctc gccggaaacc ccgtgcaccg cgagccctac
      661 gaggtcacgt gggacgcgtc ggccgctcag aagggcggct ttgcgacctt catggacaag
      721 gagatccacg accagccggc ggccgtggcc gacaccctgc gcgggcgact gaatgagcag
      781 ggacgcctca aactggatga gggactcatc gcggagtcgg tgctttcgtc tatcgacaag
      841 atcatcgtga tcgcctgtgg cacggccgcc tacgcgggaa tggtcgcgcg gtacgcaatc
      901 gagcactgga cccgcatccc cgttgaggta gagctggcgc acgaattccg ctatcgcgat
      961 cccgtgctct cgatccgcac actcgtcgtg gccatgagcc agtccggcga gacgatggac
     1021 accatcatgg cgttgcgaca cgcgcagcag cagggctcac ccgtcgtggc gattgtgaac
     1081 gtgcagggct cgacgatcgc gcgggaggcc gacgccgtgc tgtacacccg cgcggggccg
     1141 gagatcgcgg tcgcgtcgac caaggccttc ttggcgcaaa tcgcggccgc ctacctgctg
     1201 ggtatctacc tcgccgaact gcgcggcaac aagtttcccg acgagattgc ggcgctcttg
     1261 tccgaactcg acgccatgcc ggccaagatc cagcgggtgc tcgacaccag cgagccgcta
     1321 cgcgaactcg cgaggtccat ggtggatgag cgcacggtgc tgttccttgg gcgccatgtc
     1381 ggctatccgg tggcccttga gggagcgctc aagctcaagg agttggccta cattcacgcc
     1441 gagggtttcg ctgcgggtga gctcaagcac ggccccatcg cgctcgtgga gcccggacag
     1501 ccggtcttca tcgtgctgcc cagcccgcgc ggtcgcgagg tgctgcattc caaggtgatc
     1561 tcgaatatcc aggaggtgcg cgctcgcggc gcccgcacca tcgtcatcgc ggaagagggc
     1621 gacacggcgg cagccgagca cgccgaccac ctcattacgg tgccagtcac ccccacgctg
     1681 ctcatgccac tcgtcaccgt tgtcccgttg cagatcttcg cgcacgaatt ggccagcgcc
     1741 aagggcttcg acgtggacca gccacgcaac ctggccaagt ccgtcacggt cgagtagccg
     1801 tggccgtcgt cggagtgggc atcgacgtag tggagttgga gcgcttcgac cgcgcggtcg
     1861 aacgcacgcc ccgatttgcc gagaaggtgt ttaccccttc ggaacgacaa ctgggccgcc
     1921 actcgatggc cgcccgatgg gccgcgaagg aggccctcgc caaggcactg ggcgcccctg
     1981 ggggactgtc gtggcaagac gccgaggtgg tgcgggaccc ctcgggtcag cctcgattcg
     2041 tgatctccgg aaccgtcgcg gcgagagccg ccgaactggg catcgccacg ctgcacgtgt
     2101 ccctatccca cgacgcgggc atcgcgtcgg cagttgtcat cgcggaggca tcatgagctc
     2161 actcaaggtt gtggtcgatt ccgcagcgat cacccacaat gtgcggaccc tcgggaaaat
     2221 gaccgaggcg caggtcatgg cagtggtcaa ggccgacggc tacgggcatg ggctgataga
     2281 ggctgccgcc gccgcgcgcg cgggaggtgc cgcgtggctt ggcgtggcgc aaccgcacga
     2341 ggcgctcgct ttgcgtgccg ctggcgacac cggacggatg ctgacgtggc tttacggccc
     2401 ggagtttcct gcggaggaac tcatggcggc cgacgtcgat ctctccgtga actcgccgga
     2461 ggtcttgggt cgcatcgtga cggcgtcgcg cgacggttcg ccgtcgcccc gcatccacat
     2521 tgcggttgac accgggcttg gtcgcgaggg cgtgacgatt gcgaaccttc ccggcctgct
     2581 ggagctggcg aaagctgtgg agcgcgaggg gctggtgcgg gtagtgggca tgtggtcgca
     2641 cctcgcgtgg gcggacgctc cgggacaccc cacgatcgac ctgcaggcgc agcacttccg
     2701 gcaggcgctg gtgatggctc aggatgcctc gatcgcgctg gaggtgcggc acctggccaa
     2761 ctctgcttgc acgctgacca ggcccgacct gcactttgac ctggtccgcc cggggatcgc
     2821 catctatggc cttccaccga tcgaggatcc ggagggtcag aactttggtc ttcggcccgc
     2881 catgagcgtg acgtcgcaga tcatccttgt gaaggacgtc ctggcggggc agggagtgag
     2941 ttacggccac gagtacgtca cgacgtaccc gacgacgctg ggacttgtca gcgccggcta
     3001 tgccgacggc gtgttccgtg cggccgggtc cgtcgcggag gtcgcggtca agggcaggcg
     3061 ctacctcatc gcgggtcggg tgtgcatgga tcagttcgtc atcgatctcg gccccgctac
     3121 ggaggtgtgc gtgggtgacg aggtcacgct catcggcccc ggcgggccca gcgctcgcga
     3181 ctgggccacc gcggtgggca cgatcgacta cgagatcgtc tgccgcttcg ggggactgag
     3241 ggccaaggag agcgcgtgaa gtatcgcgtt cccaacggcg aggcgatgcg ctcgctcggc
     3301 cgagaattgg cgggcgtatt caccgctggc gaccttgtga tcttgtcggg cggactgggt
     3361 gcgggtaaga cgaccctggt ccagggcatc ggcgcgggac tggatgtccg tggccaagtc
     3421 gcgagcccca ccttcatcat ctctcgcgtc cacgagccga ccgcaggcgg gcccgcgctc
     3481 gtgcacgttg acgcgtaccg gctgggctcg ctggtggaac tcgaccacct cgaccttgat
     3541 gcgtcggcag acgaggcggt gacggtcgtc gagtggggcg agggcaaggc agaggcgctc
     3601 gccgagggcc acgcgctcat cacgatccac cggcccaggg gggccttggc cgagggcgaa
     3661 gacgccgccg cgggtgcccg caccgtcacc atcacgacga cgggcaacgc ctggaacggc
     3721 cgctcctggc ctgcggaagg gtggaccgca tgattgtttc tcttgacacc tcttccgcga
     3781 tctccgtggt gattgccgac gccggggcga ctgaggcgct ttcgagcagg tcggtctttg
     3841 cgcctcgcgg ccacgccgag caactcagcg acttggtgcg acaagccatg gaagaggcgg
     3901 gggtggcgcg ccccgatgtg accggcatcg tggtgggcac
//
LOCUS       DASRKH010000042         2128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_21412, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000042 DASRKH010000000
VERSION     DASRKH010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21412"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VFC06_00715"
     CDS             <1..373
                     /locus_tag="VFC06_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HZL80460.1"
                     /translation="GGYDLERRTGRIFSFDVTGGRYEETEYHAVGSGAVFARGSLKKL
                     WRSGMTAADAVRVAVHALVDASDDDSATGGPDASRQIWPIVATVTEAGYLRVSADTLA
                     QVSAEIEAKRRAADATGGEPR"
     gene            370..1125
                     /gene="prcA"
                     /locus_tag="VFC06_00720"
     CDS             370..1125
                     /gene="prcA"
                     /locus_tag="VFC06_00720"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HZL80461.1"
                     /translation="MNMPFYVSPEQLMKDRADYARKGIARGRSVVVLAYDDGIAFATE
                     NPSRALHKVSEIYDRIAFAAVGKYNEFENLRVAGVRYADLRGYSYDRADVSARALANA
                     YAQTLGTVFTTESKPLEVELVVAEVGRTPDDDQIYRLSYDGSVADEHGHVVMGGQAEH
                     LSERLSTQWRAGMGLADVVKVAVAVLGTQPDGEDRVLQPGQLEVAVLDRTRPRRAFRR
                     LAAPQLADLLGRDDVPEVDPAAPEGDGPPTEQG"
     gene            1129..>2128
                     /gene="pafA"
                     /locus_tag="VFC06_00725"
     CDS             1129..>2128
                     /gene="pafA"
                     /locus_tag="VFC06_00725"
                     /EC_number="6.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA];
                     GO_process: GO:0070490 - protein pupylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pup--protein ligase"
                     /protein_id="HZL80462.1"
                     /translation="MDRRIFGIETEYGVTCAVDSGRGLSADEVARYLFRKVVAWGRSS
                     NVFLRNGSRLYLDVGSHPEYATAECDEVPQLVAHDRAGERVLEGLVVDAQQRLEHEGV
                     PGTIHLFKNNTDSAGNSYGCHENYLVRRQGDFARLADVLVPFLITRQVLTGAGKVLPG
                     PRGATYCLSQRADHIWESVSSATTRSRPIINTRDEPHTDAEHYRRLHVIVGDSSMSET
                     TTLLKVGATDLILRLIEAGIPLRDLTLENPMRAIREISHDLTGTTAVSLANGRTASAL
                     DIQDEYLAWVQTYVTEHVEITPVIKQVLELWERGLRAVRTADLSLVDRELDWVIKHR"
BASE COUNT          324 a          741 c          763 g          300 t
ORIGIN      
        1 cggtggctac gacctggagc gccgcaccgg ccggatcttc tccttcgacg tgacgggcgg
       61 tcgctacgag gagaccgagt accacgcggt cggctcaggg gccgtgttcg cgcgcgggtc
      121 gctgaagaag ctgtggcggt ccgggatgac cgccgctgac gcggtgcggg tggcggtgca
      181 cgccctggtc gacgcctccg acgacgactc cgcgaccggc ggtcccgacg cgtcccggca
      241 gatctggccg atcgtcgcga cagtgaccga ggcgggctac ctgcgggtgt ccgccgacac
      301 ccttgcccag gtgtccgccg agatcgaggc caagcgtcgg gccgccgatg cgacgggagg
      361 tgagccgcga tgaacatgcc gttctacgtc tcgcccgagc agctgatgaa ggaccgggcc
      421 gactacgccc ggaagggcat cgcgcgcggt cgctccgtcg tcgtgctcgc ctacgacgac
      481 gggatcgcct tcgcgacgga gaacccgtcc cgtgccctgc acaaggtgtc cgagatctac
      541 gaccggatcg ccttcgccgc ggtcggcaag tacaacgagt tcgagaacct gcgggtggcc
      601 ggcgtccgct acgccgacct gcgcggctac tcctacgacc gcgccgacgt cagcgcgcgg
      661 gccctggcga acgcgtacgc ccagaccctt gggaccgtgt tcaccaccga gtccaagccg
      721 ctggaggtcg agctggtcgt cgccgaggtc ggcaggacgc cggacgacga ccagatctac
      781 cggctctcct acgacgggtc cgtggcagac gagcacgggc acgtcgtgat ggggggtcag
      841 gccgagcacc tctcggagcg gctgagcacg cagtggcggg ccggcatggg ccttgccgat
      901 gtcgtcaagg tggccgttgc ggtgctcggc acgcagcccg acggcgagga ccgtgtgctg
      961 cagcccggcc agctcgaggt cgcggtgctc gaccggacgc gcccgcgcag ggcgttccgg
     1021 cgcctcgccg caccgcagct ggcggacctt ctcggccgcg acgacgtccc ggaggtggac
     1081 ccagccgctc ctgagggcga cggcccgccg accgagcagg gctgagcggt ggaccgacgg
     1141 atcttcggga tcgagacgga gtacggcgtc acctgtgcgg tggacagcgg gcgtggcctg
     1201 tcggccgacg aggtcgcccg ctacctgttc cgcaaggtgg tcgcctgggg ccgttcgtcc
     1261 aacgtgttcc tgcgcaacgg ctcgcggctg tacctcgacg tcggctcgca cccggagtac
     1321 gcgacggcgg agtgcgacga ggtgccgcag ctcgtcgcgc acgaccgcgc gggggagcgg
     1381 gtgctcgagg ggctcgtcgt cgacgcccag cagcggctgg agcacgaggg tgtcccgggc
     1441 accatccacc tgttcaagaa caacaccgac tcggccggca actcctacgg ctgccacgag
     1501 aactacctgg tccggcgtca gggcgacttc gcgcggctcg ccgacgtcct ggtgccgttc
     1561 ctgatcaccc gtcaggtgct gaccggggcc gggaaggtgc tacccggtcc gcggggcgcg
     1621 acctactgcc tgtcccagcg cgccgaccac atctgggagt cggtgtcgag cgcgacgacc
     1681 cggtctcggc cgatcatcaa cacccgcgac gagccgcaca ccgacgccga gcactaccgg
     1741 cgcctgcacg tcatcgtcgg tgactcttcg atgtcggaga cgaccacgct gctcaaggtc
     1801 ggtgccaccg acctgatcct gcgcctgatc gaggcgggca tcccgctgcg ggacctcacc
     1861 ctggagaacc cgatgcgggc gatcagggag atcagccacg acctcacggg cacgacggcg
     1921 gtcagcctgg ccaacggacg gaccgcctcg gcgctggaca tccaggacga gtacctggcc
     1981 tgggtccaga cctacgtgac cgagcacgtc gagatcacgc cggtgatcaa gcaggtcctg
     2041 gagctctggg agcgaggact ccgggcggtc cgtaccgccg acctctcgct ggtggaccgg
     2101 gagctggact gggtgatcaa gcaccggc
//
LOCUS       DASRKH010000043         2172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_61550, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000043 DASRKH010000000
VERSION     DASRKH010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61550"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VFC06_00730"
     CDS             complement(<1..268)
                     /locus_tag="VFC06_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80463.1"
                     /translation="MIRTWYTAAVAPRISILRLLPTAGRTLVTTLVTLNLVLGLLPVA
                     FVVATSIVVGHVPAAVTGGIGSPAWDTLVRDFLLAAVAFCGQQLL"
     gene            complement(330..569)
                     /locus_tag="VFC06_00735"
     CDS             complement(330..569)
                     /locus_tag="VFC06_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019134913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004658 - propionyl-CoA carboxylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase epsilon subunit"
                     /protein_id="HZL80464.1"
                     /translation="MNGAGPGADGRARVRVVRGAPDDLELAALVAGLVAASARSDDRA
                     EPATRSVWHDRARAMRGDPVRAAPGSDAWRWSLRG"
     gene            complement(566..1120)
                     /locus_tag="VFC06_00740"
     CDS             complement(566..1120)
                     /locus_tag="VFC06_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80465.1"
                     /translation="MNDHDNDHDEHGDNPLHGVDPLQGVDPSDLHGVVDALSASLHEY
                     IDAAVGVRAEFGAHEADEDPRILALESRVSNLNAELYDVLHDRLGLHSDLTGMTWDDG
                     EEGDADLPPQEEELDTFHLGFVVGPPAGTSDLSMDSVLGIVDDGGAEIARRLTDRGFD
                     VTEWGASRGVPVEFEPDDDEDDIE"
     gene            complement(1146..>2172)
                     /locus_tag="VFC06_00745"
     CDS             complement(1146..>2172)
                     /locus_tag="VFC06_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HZL80466.1"
                     /translation="FIVMADGTSNMFITGPDVIRAVTGEDVGFEELGGATTHNTKSGV
                     AHYLAADENDALDYVRSLLSYLPANNLTDPPTYAHESGLGVTAEDEELDTLVPDSDSQ
                     PYDMRTVVEHVLDEGVLLEVQPLYAQNILIGFGHVEGHPVGIVANQPLAMAGTLDINA
                     AEKAARFVRTCDAFNVPVLTFVDVPGFLPGTDQEWNGIIRRGAKLIYAYAEATVPLVT
                     VITRKAYGGAYIVMGSKQLGADVNLAWPTAQIAVMGAGGAVNILQRGALKTVATAGGD
                     VEAERARLTAEYEDAIVNPWDAAERGYVDAVIAPSRTRGEIVRALRLLRTKRAAMVPR
                     KHGNIPL"
BASE COUNT          291 a          751 c          807 g          323 t
ORIGIN      
        1 cgagcagctg ttgaccgcag aaggcgacgg ccgcgagcag gaagtcccgg acgagcgtgt
       61 cccatgcggg cgagccgata ccgcccgtga cggccgccgg gacgtgcccg acgacgatgc
      121 tcgtggcgac gacgaacgcc accggcagga gcccgagcac gaggttgagc gtgaccagcg
      181 tggtgaccag ggtgcggccg gcggtgggca gcagccggag gatcgagatc cggggggcga
      241 cggctgcggt gtaccaggtg cggatcacga ggtgaggcta gcggtcggac gccgcccgga
      301 gtgtggtgct tcgcgacgtg cgaagcccgt cagccgcgca ggctccagcg ccaggcgtcc
      361 gagcccgggg cagcccgcac cgggtcgccc cgcatcgctc gggcccggtc gtgccacacc
      421 gagcgcgtcg cgggctcggc gcggtcgtcg gaccgggccg acgcagcgac cagcccggcg
      481 acgagcgcgg cgagctcgag gtcgtcgggt gcgccgcgca cgacccgcac gcgcgcgcgg
      541 ccgtccgcgc cgggcccggc tccgttcact cgatgtcgtc ctcgtcgtcg tccggctcga
      601 actcgaccgg cacgccccgg gacgctcccc actcggtcac gtcgaaaccg cggtcggtga
      661 ggcggcgggc gatctcggca ccgccgtcgt ccacgatgcc gagcaccgag tccatcgaca
      721 ggtccgaggt gcccgcgggt ggtccgacga cgaagcccag gtggaacgtg tcgagctcct
      781 cctcctgcgg gggcaggtcg gcgtcgccct cctcaccgtc gtcccacgtc atgccggtga
      841 ggtccgagtg caggccgagc ctgtcgtgca ggacgtcgta cagctccgcg ttgaggttcg
      901 acacgcgcga ctcgagcgcg aggatgcgcg ggtcctcgtc ggcctcgtgc gcgccgaact
      961 cggcccgcac gccgacggcg gcgtcgatgt actcgtgcag gctcgccgac agcgcgtcga
     1021 cgacgccgtg caggtcgctc gggtcgaccc cctgcagggg gtcgaccccg tgcagggggt
     1081 tgtcgccgtg ctcgtcgtgg tcgttgtcgt ggtcgttcat ggtgtcctca cggctcgagt
     1141 ctgcctcaca gggggatgtt cccgtgcttg cggggcacca tcgcggcgcg tttggtgcgc
     1201 agcagccgca gcgcgcggac gatctctccg cgggtgcgcg acggcgcgat gacggcgtcg
     1261 acgtagcccc gctcggcggc gtcccacggg ttgacgatcg cgtcctcgta ctcggccgtg
     1321 agccgcgcac gctcggcctc cacgtcccca cccgccgtgg cgacggtctt gagcgcgccg
     1381 cgctgcagga tgttcaccgc gccacccgcg cccatgacgg cgatctgggc ggtcggccag
     1441 gcgaggttca cgtcggcacc gagctgcttg gatcccatca cgatgtacgc gccgccgtag
     1501 gccttgcgcg tgatgacggt cacgaggggg acggtcgctt ccgcgtacgc atagatgagc
     1561 ttcgcgccgc gccggatgat gccgttccac tcctggtcgg tgcccggcag gaagccaggg
     1621 acgtccacga acgtgagaac cgggacgttg aacgcgtcgc acgtccgcac gaaccgcgcg
     1681 gccttctcgg cggcgttgat gtcgagcgtc ccggccatcg cgagcggctg gttggccacg
     1741 atgccgaccg ggtgaccctc gacgtgcccg aacccgatga ggatgttctg ggcgtacagc
     1801 ggctggacct cgaggaggac accctcgtcg agcacgtgct cgacgacggt ccgcatgtcg
     1861 tacggctggg agtcggagtc cgggacgagc gtgtcgagct cctcgtcctc ggccgtcacg
     1921 ccgaggccgg actcgtgcgc gtacgtcggg gggtcggtga ggttgttcgc cggcaggtac
     1981 gacagcagcg agcgcacgta gtcgagcgca tcgttctcgt cggccgcgag gtagtgcgcg
     2041 acgcccgact tcgtgttgtg cgtcgtggcg ccgccgagct cctcgaagcc gacgtcctcg
     2101 ccggtcaccg cgcggatcac gtcggggccc gtgatgaaca tgttggaggt gccgtcggcc
     2161 atcacgatga ag
//
LOCUS       DASRKH010000044         2681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_53228, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000044 DASRKH010000000
VERSION     DASRKH010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53228"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VFC06_00750"
     CDS             complement(<1..301)
                     /locus_tag="VFC06_00750"
                     /inference="COORDINATES: protein motif:HMM:NF017470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HZL80467.1"
                     /translation="MSFGQAIKSVFSQYATFTGRARRSEYWWFYLFSLLVSLPVQIFF
                     FVMYLAAFAPVLESADSNGQYSEVALEDIDWGLFAVGIVPMIIVGLALFLPSLAVT"
     gene            413..1729
                     /locus_tag="VFC06_00755"
     CDS             413..1729
                     /locus_tag="VFC06_00755"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZL80468.1"
                     /translation="MSYSSDLAHLWKSQGFRRLTRVRVVSQAGDGAFQFGIATAFFFS
                     PENATTPADIAIGFAVLFAPFTLIGPFVGPFIDRWHRQRIVLVGNMVRLVMVGMIVGA
                     VVVGAPRISLYALALATLSVNRFLLAALSAGIPQVVDPEDLLAANAIMPTLGTIAAAL
                     GGTIGGIVAFVVPGVSDDHKALLALGAAGLAFGLSSWAATSLSKRSLGPVRLLDGDHV
                     VAETAHILIELRDGYRHLRERATPFHALVIMAAARLLYGLMFVASILISRLVLGDSSQ
                     PEDAIGQFAIVVGFAAIGFGLAAVITPAFSHRVARHDWIIACLLLGAVGQSLLAISSA
                     SWALLGAAVIVSFSVQGGKIAVDTIVQRDTDDAFRGRAFALYDVAYNTAFITSAVIGA
                     LVLPQNGYSHVVMAALVVAYLAFAFAYSRAPREPRLASDSDTSPAR"
     gene            complement(1738..2271)
                     /locus_tag="VFC06_00760"
     CDS             complement(1738..2271)
                     /locus_tag="VFC06_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011776868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HZL80469.1"
                     /translation="MASALSGPHPPTNLRLDVLDERILWELTKDARLSNAALAERLHV
                     SASTTHARVRALRDAGVIRSSHIEVDYEALGLTVQGLVAVRLRAQARPQIKTYAREVI
                     QLPNVLSVYFVGGDVDFLIHAACTSTTQLRDFVASHLSMDPVVASTETQIVFDHLIGA
                     EHKQGADGLAAMRRSLA"
     gene            2399..>2681
                     /locus_tag="VFC06_00765"
     CDS             2399..>2681
                     /locus_tag="VFC06_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011776869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HZL80470.1"
                     /translation="MRIGIPAEVKNNETRVAATPSGVHALAASGHDVLVQAGAGLGSS
                     FTDEEYVNAGASIGTADDAWGAQMVLKVKEPIGAEYGYLRPDLTLFTYLH"
BASE COUNT          422 a          938 c          857 g          464 t
ORIGIN      
        1 ccgtcaccgc gagcgaaggc aggaagagcg cgaggcccac gatgatcatg ggaacgatcc
       61 ccaccgcgaa cagcccccag tcgatgtcct ctaaggcgac ttcggagtac tggccgttgg
      121 agtcggcgct ctcgagcacc ggggcgaagg ccgcgaggta catgacgaag aagaagatct
      181 gcacggggag agacacgagg agggagaaaa ggtagaacca ccaatactcc gagcgccgcg
      241 cgcgtcccgt gaacgtcgcg tactgagaga acaccgactt gatcgcctga ccgaacgaca
      301 tcgaccccac ctacctttca aggcaccacg ctagcgccga tcccgggatc acacgaagga
      361 accactcccg tcctgggcgt gcggtgccga tgccacgccc tagcctggtg ccatgtctta
      421 ctcgtccgac ctggcccacc tgtggaagtc acagggtttt cggcgcctca cccgcgtgcg
      481 cgtcgtctcg caggctggcg acggggcctt ccagttcggc atcgccaccg ccttcttctt
      541 ctccccagag aacgccacca cgcccgccga catcgcgatc ggctttgccg tgttgttcgc
      601 gccgttcacg ctgattggcc cgttcgtcgg gcccttcatc gaccgctggc atcggcaacg
      661 cattgtgctg gtaggcaaca tggtgcgcct tgtgatggtc gggatgatcg tgggggcggt
      721 cgtggtgggg gctccgcgca tctccttgta cgcgctcgcg ctcgccacgc tgtccgtcaa
      781 tcgcttcttg ctcgcggccc tgtccgcggg aatcccgcag gtggtcgacc ctgaggacct
      841 gctggccgcg aacgcgatca tgccaacgct cggaacgatc gccgcggcgc tgggcgggac
      901 gatcggcggc atcgtcgcgt ttgtcgtgcc gggggtctcg gacgaccaca aggccttgct
      961 cgcgctcggc gcggccggac tggcgttcgg gttgtcctcg tgggccgcca cctcgttgtc
     1021 caagcgctcg ctcggtcccg tccggctgct ggacggcgac cacgtggtgg cggagaccgc
     1081 gcacattctg atcgagcttc gcgacggcta tcggcacctg cgcgaacgcg ccaccccgtt
     1141 ccacgcgctc gtcatcatgg cggccgctcg actcctgtac ggactgatgt tcgtcgcgtc
     1201 gatcctcatc tctcgcctgg tgctggggga ctcctcccaa cccgaagacg ccatcggaca
     1261 gttcgcgatc gtggtcggct tcgcggccat cggattcggc ttggcggccg tgatcacccc
     1321 ggccttctcc caccgcgtcg cgcgccacga ctggatcatc gcgtgcctgc tgctcggagc
     1381 cgtcggccag tccctgcttg ccatcagctc cgcgtcgtgg gcgctgctgg gcgccgcggt
     1441 catcgtcagc ttctccgtac agggcggcaa gatcgcggtc gacaccatcg tccaacgcga
     1501 cacggacgat gccttcaggg gccgcgcctt cgccctgtat gacgtcgcat acaacacggc
     1561 cttcatcacg tctgccgtca tcggcgcctt ggtattgccc cagaatggct actcccacgt
     1621 ggtgatggcc gccctcgtgg tcgcctacct cgcgttcgcc tttgcgtact cgcgggcgcc
     1681 gcgggagccg cgtctcgcct ccgactccga tacctccccc gctcgctagt cgtcgcccta
     1741 ggcgagggac ctgcgcatcg cggcgagccc atcggcgcct tgcttgtgct ccgcgccgat
     1801 aaggtgatcg aacacgatct gcgtctccgt cgacgcgacc accgggtcca tgctcaggtg
     1861 gctggccacg aagtcgcgca actgcgtcgt cgacgtacac gccgcgtgga tgaggaagtc
     1921 gacgtcgccg cccacgaagt agaccgagag cacgttgggc aactggatca cctcgcgggc
     1981 gtacgtcttg atctgggggc gggcctgcgc gcgcagccgc accgccacaa gcccctgcac
     2041 cgtcaggccg agcgcctcgt agtccacctc gatgtgggag gaccggatga ctccagcgtc
     2101 gcgcagggcg cgcacccgcg cgtgggtggt cgaggccgag acgtgcaggc gctccgcgag
     2161 ggcggcgttg gacagtcgcg cgtccttggt gagctcccag aggatgcgct cgtcgagcac
     2221 gtcgagccga agattcgtcg gcggatgagg tcccgagagc gcagaagcca tgggtttctt
     2281 ctccattcct caccgatctg ccaaggatat gcgtacccgg ttgcaatcgg cgcagaatct
     2341 tcgacacttg aagggtggaa cgctccccct agcgcccacg agcatcgagg agaacgccat
     2401 gcgcatcgga atccctgccg aggtcaagaa caacgagacc cgcgtcgccg cgaccccaag
     2461 cggcgtgcac gccctcgccg cctccggaca cgacgtcctt gtccaggcgg gcgccgggct
     2521 cggcagctcc ttcacggacg aggagtacgt gaacgctggc gcgagcatcg gcaccgcgga
     2581 cgacgcgtgg ggcgcgcaga tggtgctcaa ggtcaaggag ccgatcgggg ccgagtacgg
     2641 ctacctgcgc cccgacctca ccctgttcac gtaccttcac c
//
LOCUS       DASRKH010000045         2946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_61489, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000045 DASRKH010000000
VERSION     DASRKH010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61489"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VFC06_00770"
     CDS             <1..283
                     /locus_tag="VFC06_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80471.1"
                     /translation="EYPVETTILIPEGSPMFEPLLAAAEGDDSWVTARIAEMKAVVTR
                     PKDGSQGPWIDARNNGAWDMIFEPRQQDFPAAPVGDEEIELAVALYPNP"
     gene            complement(301..981)
                     /locus_tag="VFC06_00775"
     CDS             complement(301..981)
                     /locus_tag="VFC06_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80472.1"
                     /translation="MSTPTCSAPVWGHRSDYSRAQCPSPACNGGRPTLAQVALASLPP
                     RILTRLDAYVPEQGFFEPDDEESAELERDLFRRGLTPFGFEVWSHPGQDGRHVSEGVQ
                     VYDFPHNDYLAVYVGSALNVRAPREVDGRKVSVGRLIDGNRLSLGARSDDTYPMDEWS
                     LRPRPAAAEVVAESVVDVESPEFQSYYDKNYEWLAGRCDPEPGEDWDKHMLETAKFYF
                     AAEQSETS"
     gene            complement(978..1658)
                     /locus_tag="VFC06_00780"
     CDS             complement(978..1658)
                     /locus_tag="VFC06_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80473.1"
                     /translation="MTDAAVAVGLGVSLLLVELGRSVPAWLRVRRTQSVDGYSAVSQG
                     ILLGTAPAWLLVAVIEGAPWIFAATALWAALHVALVAEGFRVDPAFGLRALRVGGVSL
                     ATAGVAALVGATLFDSLGLALGLMVVAATAGYSLPALVAGMRSASTRGLSVIELAINV
                     FEGVVYVLAGLGVVALVASSQGALSYVLFGAVAVVSNAPRLVRTSYRRVRGLDAPPVL
                     SAADTLVV"
     gene            complement(1655..2251)
                     /gene="orn"
                     /locus_tag="VFC06_00785"
     CDS             complement(1655..2251)
                     /gene="orn"
                     /locus_tag="VFC06_00785"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003765.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HZL80474.1"
                     /translation="MSTHENAAIEPPDILVWCDEETTGLVAHHEHLLEVAVLVTDVDL
                     NLLDEVGFERIVHYDQGTVRRFRDNAAPVVRGMHDASGLWDRLSSGQAVDVVDQEMLA
                     YIKQFAPNYRQARFAGNSLRLDMNFADEWLPETAAHMHYQFLDVSGMAFLGKKWAGVP
                     LMDKAYGHTAMADIRESLAELKYLRAHGLFARQHAAVA"
     gene            2398..>2946
                     /locus_tag="VFC06_00790"
     CDS             2398..>2946
                     /locus_tag="VFC06_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80475.1"
                     /translation="MTDTQTRSATVSRANVRGIMLDGKFTGVSRWAALDTDALTWECK
                     KPDWFEPTVTDLRWAEESGEPRNAIFMGGDAVLLHGYALTLRDAIEDTGASFSFDRFL
                     PGFRVKDPDQYADGLRDICKTYNICVSPQLEGHLDGLDQAVHAVAGTAADALSVGFAV
                     PKNFASGRVLKEHQEDAVLTMAH"
BASE COUNT          570 a         1003 c          935 g          438 t
ORIGIN      
        1 cgagtacccg gtcgagacga cgatcctgat ccccgaaggc agccccatgt tcgagccgct
       61 cctcgcggcg gccgaaggtg acgactcgtg ggtgacggca cgcatcgcag aaatgaaggc
      121 ggtcgtcacg cgacccaagg acggcagtca gggtccgtgg attgacgcga ggaacaacgg
      181 cgcatgggac atgatcttcg agccgcgtca gcaggacttc cccgcagcgc cggtcggcga
      241 cgaggagatc gaactcgcgg tggctctgta ccccaacccg taggtccgac caccccgcga
      301 ctacgaggtc tcggactgct ccgcggcgaa gtagaacttc gcggtttcga gcatgtgctt
      361 gtcccagtcc tcgcccggct ccgggtcaca ccggcctgca agccactcgt agttcttgtc
      421 gtagtaactc tggaactccg gggactccac atcaacaacg gactcggcca caacctcggc
      481 cgcggcaggg cgagggcgca acgaccactc gtccatcggg tacgtgtcgt cggagcgcgc
      541 ccccaaggac agacggttcc cgtcgatgag ccgaccgacc gacaccttgc gcccgtcaac
      601 ttcgcgaggc gcccggacgt tgagggccga accgacgtag accgcgaggt agtcgttgtg
      661 cgggaagtcg tacacctgca cgccctccga aacgtgacgc ccgtcttggc cggggtggct
      721 ccacacttcg aacccgaaag gtgtcaggcc gcggcggaac aagtcccgct cgagttcggc
      781 ggactcctcg tcatccggct cgaagaaccc ctgctccggc acgtacgcgt ccagccgcgt
      841 caagatccgc ggcggcagtg acgcgagcgc cacctgagcc aaagttggcc tgccgccatt
      901 gcacgccggc gacgggcact gcgcacggga gtagtcggac ctgtgacccc ataccggggc
      961 gctgcatgta ggagtgctca taccaccaag gtatccgcgg cgctcaacac gggcggggcg
     1021 tcgagaccac gcacccgacg gtatgaagtg cggacgagac gaggcgcatt ggagacgacg
     1081 gccacagcgc cgaagagcac gtaactgaga gccccttgcg acgaggcgac cagggccacg
     1141 accccgagcc cggcaagcac gtagacgacg ccctcgaaca cattgatggc cagttcgatg
     1201 acgctcagcc cccgagtcga cgcggagcgc atccccgcca ccaacgccgg gagcgagtac
     1261 cccgcggtcg ccgcgacgac catcaaaccc agtgcgagcc ctagcgagtc gaagagtgtt
     1321 gcgcccacca gtgctgcgac gcctgccgta gcgagcgaga cgccaccaac cctcagcgcc
     1381 cgaagaccga aagcgggatc gacccggaaa ccctcggcga cgagcgccac atgcagtgcg
     1441 gcccacagtg cggtggccgc gaagatccac ggggcgccct cgatgactgc gacaaggagc
     1501 catgcaggag cggtgcccag aaggatgccc tggctgaccg cgctgtaccc gtcgacggac
     1561 tgagtgcggc gaacccgcaa ccaggcaggg acgctgcgac ccaactcgac cagcagcagg
     1621 gagacgccga gacctaccgc gaccgcggca tctgtcacgc gacagccgcg tgctggcgag
     1681 cgaacagccc gtgcgcgcgc aggtacttca actcggcaag cgactctcgg atgtcagcca
     1741 ttgcggtgtg accgtatgcc ttgtccatca gcggaactcc ggcccatttc ttgcccagga
     1801 acgccatccc ggagacgtcc aggaattgat agtgcatgtg cgctgcggtc tccggcagcc
     1861 actcgtcggc gaagttcatg tccaagcgca gcgagttgcc ggcgaagcgc gcctggcgat
     1921 agttcggggc gaactgcttg atgtaggcga gcatctcctg gtcgaccacg tcgacggcct
     1981 gaccggacga aagccggtcc cacaaaccag aggcgtcatg catcccccgc acgacggggg
     2041 cagcgttgtc gcgaaagcgc cggaccgtgc cctggtcgta gtggacgatg cgctcgaacc
     2101 cgacctcgtc gagcaggttc aggtcgacat cggtgacgag gacggcaact tccagcaggt
     2161 gctcgtggtg ggcaaccagt ccggtggtct cctcgtcgca ccagaccagg atgtctggcg
     2221 gctcgatcgc tgcgttctcg tgtgtgctca tgtggtcaga ctagccaaag tgtgtgacac
     2281 gaaatgcggt cagccgttat gggccctggc cgccgggacg agcacgaacc gcaaaccctt
     2341 cgtgtgtggt agcctttggt attactacca cacaaatatg aggggtccac caggactatg
     2401 accgacactc aaacccgctc cgccactgtt tcgcgcgcca acgtgcgcgg catcatgctc
     2461 gacggcaagt tcaccggcgt cagccgatgg gccgctctcg acaccgatgc gctcacctgg
     2521 gaatgcaaga agcctgactg gttcgagccg accgtcaccg acctccggtg ggccgaggag
     2581 agcggtgagc cccgcaacgc aatcttcatg ggcggcgacg ccgtcctgct ccacgggtac
     2641 gcgctcacgc tgcgtgacgc catcgaagac accggggcgt ccttctcgtt cgaccggttc
     2701 ctccccggct tccgggtcaa ggaccccgac cagtacgccg acggtctgcg tgacatctgc
     2761 aagacctaca acatctgcgt gtccccccag ttggaggggc acctcgacgg actcgaccag
     2821 gccgtccacg ccgtcgcggg gactgcggcc gacgccctgt ccgtcgggtt cgccgtccct
     2881 aagaacttcg cgtccgggcg tgtgctcaaa gagcaccagg aagacgccgt gctgacgatg
     2941 gctcac
//
LOCUS       DASRKH010000046         2101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_12636, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000046 DASRKH010000000
VERSION     DASRKH010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12636"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /locus_tag="VFC06_00795"
     CDS             complement(<1..1145)
                     /locus_tag="VFC06_00795"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HZL80476.1"
                     /translation="MISCPALPEVRSTRDDPLACARAEGALALIISPAPDAQEDSGEA
                     EPRSGPFEPLREVAAAYGIDLEAVVADQRVGGPGSVHMIELPPLLADGGPWQSLARRV
                     ILAGAGGGDPRELRDAGTAVARAAAGLPHLAVALHAPLDAARSLALLEGLMLPAYRMP
                     RIAAGEAPGRAACPEIVVSGELDEGSIPVAAAGVWGTFLARTLAATPSNIKDPEWLAR
                     QVTDLVAAAADERLTVEVRDEDWLARRGLNAILAVGAGSASPPRLVSVRYDPAGSAPE
                     VALVGKGITFDTGGLSLKPREAMVPMKADMSGAAIVLAAVLAAARLDAPRPAVAVLAL
                     AENAFGGASCRPGDVVRTVDGTTVEIGNTDAEGRMVLADAIAWVRAE"
     gene            complement(1145..1312)
                     /locus_tag="VFC06_00800"
     CDS             complement(1145..1312)
                     /locus_tag="VFC06_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3117 domain-containing protein"
                     /protein_id="HZL80477.1"
                     /translation="MAAMKPRTGDGPLEVTKEGRGLVMRVPLEGGGRLVVELNQEEAN
                     ALGDALAAVRV"
     gene            complement(1421..>2101)
                     /locus_tag="VFC06_00805"
     CDS             complement(1421..>2101)
                     /locus_tag="VFC06_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HZL80478.1"
                     /translation="DTDWLHTDPWRVMRIQAEFVEGFGALADIGPAVSVFGSARTKPN
                     TKEYLLGRDIGRMAAEAGFAVITGGGPGIMEAANKGASTAGGVSVGLGIELPHEQGMN
                     KWVDLGINFRYFFVRKTMFVKYSTGFISLPGGFGTLDELFEALTLVQTHKVRSFPVVL
                     VGKDYWGGLLDWLASSMVESGKISDLDLDLITLVDTADEAVEAVTSGHNRVVEAALAK
                     DTGHSDGS"
BASE COUNT          296 a          795 c          716 g          294 t
ORIGIN      
        1 tactcggccc gcacccaggc gatcgcgtcg gccagcacca tgcggccctc ggcgtcggtg
       61 ttgccgatct cgacggtggt gccgtccacg gtacgcacca cgtcgccggg gcggcagctg
      121 gccccgccga acgcgttctc cgcgagcgcc agcacggcga cggcgggccg cggagcgtcg
      181 agccgggcgg cggcgagcac cgcggcgagc acgatcgccg cgcccgacat gtcggccttc
      241 atcggaacca tggcctcgcg cggcttcagc gacagccccc cggtgtcgaa cgtgatgccc
      301 ttgcccacca gcgccacctc gggtgcgctc ccggccggat cgtaccgcac gctcacgaga
      361 cgcggtggcg aggcggaccc ggcgccgacg gcgaggatcg cgttcagccc gcgccgcgcg
      421 agccagtcct cgtcgcgcac ctcgacggtg aggcgctcgt ccgcggccgc ggcgaccagg
      481 tcggtcacct gccgggcgag ccactccggg tccttgatgt tcgacggcgt cgccgcgagc
      541 gtgcgcgcga ggaaggttcc ccagaccccc gccgcggcga ccgggatgct cccctcgtcc
      601 agttcgccac tgaccacgat ctcgggacaa gccgccctgc cgggcgcctc gcccgccgcg
      661 atgcgcggca tccggtacgc cggcagcatg agtccctcca gcagcgcgag ggaacgcgcc
      721 gcgtccagcg gagcgtggag ggcgacggcg aggtgcggca gccccgctgc cgcccgggcg
      781 acggctgtgc ccgcgtcccg gagctcgcgc ggatcgccgc caccggcgcc ggccaggatg
      841 acgcgccgcg ccagggactg ccacgggccg ccgtcggcga gcagcggcgg gagttcgatc
      901 atgtggacgg aacccggacc gcccaccctc tggtccgcga ccacggcctc gagatcgatg
      961 ccgtaggccg ccgcgacctc ccggaggggc tcgaacggcc cgctccgggg ttcggcctcc
     1021 ccggaatcct cctgcgcgtc cggggccggg gaaatgatga gtgcgagtgc cccttcagcc
     1081 ctcgcgcatg ccaacgggtc gtcccgggtg gatcggacct ccgggagtgc cgggcaggag
     1141 atcatcagac gcgcacggcg gcgagggcgt cacccagcgc gttggcctcc tcctggttga
     1201 gctccaccac gaggcgcccg ccgccctcca gcgggacgcg catcacgagg ccgcggcctt
     1261 ccttggtcac ctcaagtggg ccgtctccgg tgcgtggctt cattgctgcc atggcgctcc
     1321 ccttcaatcc gagcggacga tgcgccagac ggcgcaagga ctgtgtccat tttatgccac
     1381 cgcccggtcc tctgccgatc cggccggcgg gcgcggaccc tcaggagccg tccgagtgcc
     1441 ccgtatcctt ggcgagcgcc gcctcgacca cccggttgtg cccgctggtg accgcctcga
     1501 ccgcctcgtc ggccgtgtcg accagagtga tgaggtcaag gtccaggtcg gagatcttcc
     1561 cgctctcgac catgctggac gccagccagt cgagcaggcc accccagtag tccttcccca
     1621 cgagcaccac cgggaaggag cgcaccttgt gggtctggac cagggtgagc gcctcgaaca
     1681 actcgtcgag ggtgccaaaa cccccgggga gcgaaatgaa tccggtggag tacttcacga
     1741 acatcgtctt gcggacgaag aagtaccgga agttgatgcc caggtccacc cacttgttca
     1801 tgccctgctc gtgcgggagc tcgataccca gtcccaccga gactcccccg gctgtgctcg
     1861 cgcccttgtt ggccgcctcc atgatcccgg gccctccccc ggtgatcacg gcgaaaccgg
     1921 cctcggccgc catccggccg atgtcccggc cgagcaggta ctccttggtg ttgggcttcg
     1981 tgcgggccga gccgaacacg gacacggcgg ggccgatgtc cgcgagcgca ccgaatccct
     2041 cgacgaactc cgcctggatg cgcatcaccc gccaggggtc ggtgtgcagc cagtcggtgt
     2101 c
//
LOCUS       DASRKH010000047         2076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_48111, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000047 DASRKH010000000
VERSION     DASRKH010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48111"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="VFC06_00810"
     CDS             <1..664
                     /locus_tag="VFC06_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HZL80479.1"
                     /translation="VVVRTNRLVLATPEAAGLLTAVVPGLGDLHPDRGADVVLVTLVL
                     DAPVLDAAPRGTGVLVARDVDRVRAKALTHATAKWAWLARDAGPGRHVVRLSYGVAGS
                     PVAGAAPDGTQPADARTLGDDALAALALRDASTLLGVALSPAQVVASARVAWSQALPR
                     PSAAHSATVARVRAGVAGAGGLAVCGAWVAGNGLASVVPDARRAADALLHEHRTGAQS
                     AR"
     gene            839..1614
                     /gene="hemC"
                     /locus_tag="VFC06_00815"
                     /pseudo
     CDS             839..1614
                     /gene="hemC"
                     /locus_tag="VFC06_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019481671.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
     gene            1611..>2076
                     /locus_tag="VFC06_00820"
     CDS             1611..>2076
                     /locus_tag="VFC06_00820"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HZL80480.1"
                     /translation="MTSPVRGTTTEPPAGRGPLAGLRVLVPRATDGPDPLVIALTANG
                     ADPVTVPLIQTVPPEDPTELDDVLLALGIGYYGWLAVTSGAAVPVLVDRALEIGATLT
                     SLVDGMRVAAVGPATARALRDAGVHVDLVPRGASSAADLLAAWPPLPVLSALP"
BASE COUNT          225 a          837 c          757 g          257 t
ORIGIN      
        1 cgtcgtggtc cgcaccaacc ggctcgtgct cgcgacgccc gaggccgccg ggctgctgac
       61 cgccgtcgtg cccgggctcg gtgacctgca ccccgaccgg ggtgccgacg tcgtcctcgt
      121 gacgctcgtc ctagacgccc ccgtgctcga cgcggctccg cgcgggacgg gcgtgctcgt
      181 cgcacgggac gtcgaccggg tgcgcgccaa ggcgctcacg cacgcgacgg cgaagtgggc
      241 gtggctcgcg cgcgacgccg gcccgggtcg tcacgtcgtg cgcctgtcct acggtgtcgc
      301 ggggtcgccg gtcgccggcg ccgcgcccga cggcacacag ccggccgacg cgcgcacgct
      361 cggggatgac gccctggcgg ccctcgcgct ccgggacgcc tcgacgctgc tcggtgtcgc
      421 actgtcaccc gcccaggtgg tcgcctcggc gcgggtcgcc tggtcgcagg cgctgccccg
      481 accgagcgcc gcccacagcg ccacggtcgc ccgggtccgg gcgggggtcg cgggcgcggg
      541 cggcctcgcc gtctgcggcg cctgggtcgc cggcaacggg ctggcctcgg tcgtcccgga
      601 cgcccgtcgg gccgccgacg ccctcctgca cgagcaccgc accggcgcgc agtccgcgcg
      661 gtagcgccga tcgcgtgcga cgcggacaat gctgtgcggc gggattgttc acagcacgcg
      721 ccgcggacta ttccgccggg attctgacaa tgcgtcagta aatgacgtga gaccgcacag
      781 cgggcagagc gcggcgggct ctttcgacat cgacccgatc gtgcacgaca ctggcgacat
      841 gccctcgacc gtccgtatcg gcacacgtgc gagcacgctg gccctcaccc agaccggtca
      901 cgtcgccgac gcgctgaccc gctggggcgc agaggccgtc gagctcgtcc aggtccgcac
      961 ggacggcgat cggctcaagg gctcgctggc ctcgctcggc ggcaccgggg tcttcgtcac
     1021 ggcactgcgg gacgcgctgc tcgacggccg gtgcgacgtc gccgtccact cgctcaagga
     1081 cctgccgacc ggccccgccg agggcctggt catcggcgca gtcccggcgc gcgaggaccc
     1141 gcgcgacgcg ctgtgcgcac ggcactcctg gacgctcgag tcgctgcccg agggcgcgcg
     1201 ggtcgggacg ggctctcctc gccgcgcggc ccagctgctc gccgcacgcc ccgacctcga
     1261 catcgtggac atccgcggca acgtcgacac ccgcctgcgc cgggtcgcgg ggtcgtcgag
     1321 tgcggcgagg gcgcggtgca gcggcgaacc gggctcgagg ccggctgcgc cgcacccgtc
     1381 ggtgcgctcg cgtggctctc gaccaccgga gacgtcctca cgctcgactc ggtcgtgacg
     1441 ggcaccgacg gcaagcggca gctgcgccgg tcggcgtccg ccgcgatgcc tacggcctcg
     1501 gcaggtggcg tcgacgacgc gggcgctcgc gccgccgtcg acctcggcaa ccggctcgcc
     1561 gacgagctgc tcgccgccgg cgccgccgag ctcgccgacc tgggaccgcg atgaccagcc
     1621 cggtccgcgg cacgaccacc gagcccccgg ccgggcgcgg cccgctcgcc ggcctgcgcg
     1681 tcctggtacc ccgcgcgacc gacggccccg acccgctcgt catcgcgctc accgcgaacg
     1741 gcgccgaccc ggtgaccgtc ccgctcatcc agaccgtccc gcccgaggac ccgaccgagc
     1801 tcgacgacgt gctgctcgcg ctcggcatcg ggtactacgg atggctcgcg gtgacgagcg
     1861 gcgcagcggt ccccgtgctg gtcgaccgcg cgctcgagat cggtgcgacc ctcacctcgc
     1921 tcgtcgacgg catgcgcgtc gccgccgtcg gacccgcgac ggcgcgcgcg ctgcgggacg
     1981 cgggcgtgca cgtcgacctc gtgccgcgcg gcgcgtcgtc ggccgcggac ctgctcgccg
     2041 cgtggccccc gctcccggtc ctgtccgcgc tgcccg
//
LOCUS       DASRKH010000048         2017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_9158, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000048 DASRKH010000000
VERSION     DASRKH010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9158"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..495
                     /locus_tag="VFC06_00825"
                     /pseudo
     CDS             145..495
                     /locus_tag="VFC06_00825"
                     /inference="COORDINATES: protein motif:HMM:NF014232.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
     gene            complement(755..1456)
                     /locus_tag="VFC06_00830"
                     /pseudo
     CDS             complement(755..1456)
                     /locus_tag="VFC06_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882667.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
     gene            complement(1466..>2017)
                     /locus_tag="VFC06_00835"
     CDS             complement(1466..>2017)
                     /locus_tag="VFC06_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HZL80481.1"
                     /translation="GLRYNASSLEIRYKGKTIAEVLEMTVDQALEFFGPVPALRDRLQ
                     TLADVGLGYVPLGQSATTLSGGEAQRLKLSKELSKRATGRTLYILDEPTTGLHFEDVK
                     KLMSVLQGLVDKGNTVIVIEHNLDVIKSADWVLDMGPEGGNGGGGVVAEGTPEDIATH
                     PESHTGKFLANVLGVHAQVSAPA"
BASE COUNT          335 a          633 c          675 g          374 t
ORIGIN      
        1 gacagcaacg aggtcgaggc gctcgtcctt gagcacacgt ggatcaagca gtacgacccc
       61 cgtttcaacg tcgtgttcaa ggacgacaag tcatatccgt tcctcgccgt gacgatgaac
      121 gagaagtacc cccggatgca ggtcatgcgc ggtgatcgaa agaagggcgt gcggtacttc
      181 ggtccctacg ccaagccgtg ggcgattcga gagaccgtcg acacgctcct cacggtgcta
      241 cctgtgcgga cctgcgcgcc cgcagtgttc agcaaggcgc agcgccagga ccggccatgc
      301 ctgctcggat acatcgacaa gtgtgcggcg ccgtgcgtgg gtcgcgtcag cgagcaagag
      361 caccgtgagt tggccgacaa ggtgtgccag gtgttggccg gcgacgcgaa gcccttgatc
      421 cgggagctga cgcagacgat ggagggggcg tccgcacggg aagacttcga ggttgcagct
      481 cgggcgcggg accgcctgcg ggcactgacc agcgtcttgg aacgcaacgc gatggtgttg
      541 tcgcaaggcg tcgacgtcga tgtcttcagc ctgattgacg acgagtggga agctgctgca
      601 cacgtcttct acgtgcggga cgggcgcatc accggcgaac ggggttgggt ggtcgacaag
      661 ccggagccgc tcgaggcggc gcagatggtg tcggtgttgc ttcaagaggc atacacgaca
      721 gccgactcgg tgccgcccga gatcctggtg ccggtcgcgc ccgagctgcc acctcgaagt
      781 cttcccttgc ggacgccccc tccatcgtct gcgtcagctc tcggatcaag ggcttcgcgt
      841 tgccggccaa cacctggcac accttgtcgg ccagcgcacg gtgctcttgc tcgctgacac
      901 gacccacgca cggcgccgcg cacttgtcga tgtatccaag caggcatggc cggtcctggc
      961 gttgcgcctt gctgaacact gcgggcgcgc aggtccgcac cggaagcacc gtgaggagcg
     1021 tgtcgacggt ctctcgaatc gcccacggct tggcgtaggg accgaagtac cgcacgccct
     1081 tctttcgatc accgcgcatc acctgcattc gggggtattt ctcgttcatc gtcacggcga
     1141 ggaacggata cgacttgtcg tccttgaaca cgacgttgaa gcgggggtcg tactgcttga
     1201 tccaggtgtg ctcgaggacg agcgcctcga cctcgttgcg cacgacggtc cactcgaccg
     1261 aggcggccgt cgtcaccatc agccgcgtcc tggggtgcag cgacgtcaac ggctggaagt
     1321 agttgttcag ccgcgaccgc aggttctttg ccttgccgac gtacacgacc cggccgtggg
     1381 ggtcgcggaa acggtagacg ccgggcgccg tgggaatgct gcccggcgcg gggcggtatt
     1441 cggaagggtc tggcacgatg acagcctagg cgggagcgga cacctgggcg tggacgccca
     1501 gcacgttggc gaggaatttg cccgtgtggc tctcgggatg cgtcgcgatg tcttcggggg
     1561 tgccctctgc gactactccc ccgccgccgt tgcctccctc tggacccatg tcaagtaccc
     1621 agtccgccga cttgatcacg tcgaggttgt gttcgatcac gatcacggtg ttgcccttgt
     1681 ccaccagtcc ctgcagaacg ctcatgagct tcttcacgtc ctcgaagtgc aggccggtgg
     1741 tgggctcgtc caggatgtag agggtgcggc cggtcgcccg tttgctcaat tccttggaga
     1801 gtttcaagcg ctgggcctcg ccgccggata aggtggtggc cgactgcccc agcggcacgt
     1861 atcccaggcc cacgtccgcc agggtctgga gccggtctcg taaagccggc accggcccga
     1921 agaactccag ggcctggtcc acggtcattt ccaggacctc ggcgatggtt ttgcccttgt
     1981 agcgaatctc caggctggag gcgttgtagc gcaggcc
//
LOCUS       DASRKH010000049         2252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_47817, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000049 DASRKH010000000
VERSION     DASRKH010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47817"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2252)
                     /locus_tag="VFC06_00840"
     CDS             complement(<1..>2252)
                     /locus_tag="VFC06_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="protein ndvB"
                     /protein_id="HZL80482.1"
                     /translation="DTLLPRAAAKRLVAKMAHPLNRAWFDPSTGRVTRGYSILQPRVT
                     PSLPPKEDTSAFQTVYSTQRGLDPYAFAVSDVYQDLFQEGSFAGKGIYDIDALTRALE
                     GRIPENSVLSHDLLEGNYSRSGLVTDIEVVEEHPTSYEVATSRTHRWTRGDWQLLPWI
                     LSRHEGLSPLALWKMVDNLRRSLSPVLLVAGLLVAVAVLPAPAALAWLLLTVALFLLP
                     PVLPLAPALLLRREGITKRSQVRALIDDAGQALALGALNFTFLAHQASMMVDAIARTL
                     WRIAVSHRNLLEWTTAAAAQKQAKGTVRRFVRVMAGGFVAPVALVAIAAVRGPGTLAV
                     VALPAVVWLLAPVIAQRVSRHHDRIELVASPEELDYLRSIARRTWNFFESFVTAEENH
                     LPPDNVQEQPELRVAHRTSPTNIGLYFLTTVSARDFGWIGLADAVERLEATMRTVSVL
                     EHDHGHLLNWYDTRTLEPLPPRYVSSVDSGNLAGHLIALANACAEWAADPAVGADRSG
                     GIADGVALIRQSLAELPADRAADRRATLITRLGLVDDAVAKLARAEDAAGALIAVEAA
                     LARLAEAVPPSVEVAVWVEATRRSVASLQRDAALTPDQLHSILERLAWVETQARHEFH
                     STDFRHLVDRRRELLSVGFRVDEGKLDESCYDLLASECRLGSYVAIAKDDIRTRHWFR
                     LGRSVTAAGGGAALLSWSGSMFEYLMPLLVMPTYDDTLLAQTCRAAVHRQIEYGKKRG
                     VPWGISESCY"
BASE COUNT          348 a          730 c          869 g          305 t
ORIGIN      
        1 gtagcaggat tccgagatgc cccacggcac cccgcgtttt ttcccatatt cgatctgcct
       61 atgcacagcc gccctgcaag tctgggcgag cagagtgtca tcgtaggtcg gcatcaccag
      121 aagcggcatg aggtactcga acatcgaccc ggaccacgag agcagggcag cgccgccccc
      181 cgccgccgtg accgaccgac cgagccggaa ccagtgccgc gtgcggatgt cgtcctttgc
      241 gatcgccaca tagctgccca gccggcactc ggaggcgagc aggtcgtagc agctctcgtc
      301 gagcttcccc tcgtccaccc ggaacccgac cgagagaagc tcgcgtcgcc tgtccaccag
      361 gtggcggaag tccgtcgagt ggaactcgtg ccgggcctgc gtctccaccc aggcgaggcg
      421 ttccaggatg gaatgcagct ggtccggcgt cagcgcggcg tcccgctgga gcgaggcgac
      481 ggagcggcgg gtggcctcca cccagacggc gacctcgacc gagggcggga cggcctcggc
      541 gaggcgcgcg agtgcggcct cgacggcgat gagcgcacct gccgcgtcct cggcccgcgc
      601 gagcttcgcg acggcgtcgt ccacgaggcc gaggcgcgtg atgagcgtgg cacgccggtc
      661 ggcggcgcga tccgccggaa gttcggcgag tgactggcgg atcagggcga cgccgtcggc
      721 gatgccgccg ctgcgatcgg cgccaaccgc ggggtccgcg gcccactccg cgcaggcgtt
      781 ggcgagcgcg atcaggtgcc ccgccaggtt cccgctgtcc acgctcgaga cgtagcgcgg
      841 ggggaggggt tcaagcgtcc tggtgtcgta ccagttcagg aggtggccgt ggtcgtgctc
      901 caggacggag accgtgcgca tggtcgcctc gagccgttcg acggcgtcgg ccaggccgat
      961 ccacccgaag tcgcgcgcgc tgaccgtggt gaggaagtag aggccgatgt tggtgggcga
     1021 ggtccggtgc gcgacgcgca actccggctg ctcctggacg ttgtcagggg ggaggtggtt
     1081 ctcctccgcc gtgacgaacg actcgaagaa gttccacgtg cggcgagcga tcgaccggag
     1141 gtagtcgagc tcctccgggg acgccaccag ctcgatgcgg tcatggtgcc ggctgacgcg
     1201 ctgggcgatg acgggggcca gcagccagac gacggcgggg agcgccacca ccgccagggt
     1261 gccgggcccg cgcaccgcgg cgatcgccac cagggcgacc ggggcgacga agccgccggc
     1321 catcacgcgc acgaagcgac gaacggtgcc cttggcctgc ttctgggcag cggcggccgt
     1381 ggtccactcc aggaggttgc ggtgtgagac ggcgatgcgc cacagcgtcc gcgcgattgc
     1441 gtccaccatc atggacgcct ggtgcgcgag gaacgtgaag ttcagcgccc ccagggcgag
     1501 ggcctgaccg gcgtcgtcga tgagggcgcg gacctggctc cgcttcgtga tcccctcccg
     1561 ccgcagcagg agggcgggcg cgagcggcaa cacgggcggg agcaggaaca gcgccacggt
     1621 gaggaggagc cacgccagcg ccgcgggcgc gggcaggacg gcgacggcca cgagcaggcc
     1681 cgccacgagg agcacggggg agagcgagcg gcggaggttg tccaccatct tccatagggc
     1741 gagcgggctc agtccctcgt ggcgggacag gatccacggc aggagctgcc agtcgccgcg
     1801 cgtccagcgg tgggtccggg acgtggccac ctcgtaggag gtggggtgtt cctcgaccac
     1861 ctcgatatcg gtgacgagcc cggaccggga gtagttaccc tccagcaggt cgtggctgag
     1921 gaccgagttc tcggggatgc ggccttcgag ggcgcgggtg agggcgtcga tgtcgtagat
     1981 ccccttgcct gcgaacgagc cctcctggaa caggtcctgg tagacgtcgg agacggcgaa
     2041 ggcatagggg tcgaggccgc gctgcgtgga gtagacggtc tggaaggcgg aggtgtcctc
     2101 cttgggcggg agcgacggcg tgacccgggg ctggaggatc gaatagccgc gcgtcacgcg
     2161 cccggtgctc gggtcgaacc aggcccgatt gagcgggtgg gccatcttgg cgaccagccg
     2221 cttggccgcg gcgcggggga ggagggtgtc gg
//
LOCUS       DASRKH010000050         2092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_64400, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000050 DASRKH010000000
VERSION     DASRKH010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64400"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="VFC06_00845"
     CDS             complement(<1..707)
                     /locus_tag="VFC06_00845"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZL80483.1"
                     /translation="MAEIHAARLASVDPGMSISDYLIWHGTQSPATTLYREKVGDVWV
                     DWSAAKTLEVVRGLAKGLKAAGYGAGDSIAIMSHTRLEWTLADLAIWHVGAVPVPVYE
                     TSSASQTQWILIDAGCVAAFVEDDALADVVAGARAEGDGHGVRDVWVFDNDDLGRLRE
                     RGSDLTDAEADDAVAGVGLDDVATIIYTSGTTGLPKGVELTHGNFVELSEETIALLPD
                     VVREPGASLLLFLPLAH"
     gene            complement(798..>2092)
                     /locus_tag="VFC06_00850"
     CDS             complement(798..>2092)
                     /locus_tag="VFC06_00850"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZL80484.1"
                     /translation="GPPARASPPARASPPSRARPLGGVMHVVVIGTGPAGITAAETLR
                     RLDPHGSVTALSAEPFPPYSPVALADHLLTGREGPLFWKGQDAARRLGIEERRGAVVR
                     GVDTDNREVVLDDGARLAYEGLVVASGSRLHAPLEGADLPGILDFKSLSTARSLLARV
                     RSQECRTALVVGNGFIGVELSLLLADLGVDTTIVGRRSWLMPRALDRETSAIAEAAVR
                     ARGVTLRLGVEATAFIGNPANGVRLADGSALTADLVLAATGVKPHIGHLADSAIQTDW
                     GVLVDDSLRTSAPGVVAAGDVAVTRDYMTGERYVHAIFHNAVRQAPIAAANLLGAQLT
                     YEGAEAMNSLKHLGVPIVAVGSTDNPDAVLRWGDGQQLRSVYLRDGRITGAQLAGNIR
                     SIGIYRSLMRRRIDVSPFGHRLVEPRFGLADLLFDVLP"
BASE COUNT          315 a          717 c          740 g          320 t
ORIGIN      
        1 atgtgggcga gcgggaggaa cagcagcagc gatgcccccg gctccctcac cacgtccggg
       61 aggagcgcga tggtctcctc cgacagctcc acgaagttgc cgtgtgtcag ctccacaccc
      121 ttcgggagtc cggtggtccc cgacgtgtag atgatggtgg ccacgtcgtc gagtcccacg
      181 ccggcgacgg cgtcgtccgc ctccgcgtcc gtgaggtcgc taccccgctc ccggagccgg
      241 cccaggtcgt cgttgtcgaa cacccagacg tcccggacgc cgtgcccgtc cccctcggcg
      301 cgcgcgccgg cgacgacgtc ggcgagggcg tcgtcctcga cgaacgcggc cacgcacccg
      361 gcatcgataa ggatccattg cgtctgcgac gcggacgagg tctcgtacac cggcacgggt
      421 acggcaccga cgtgccagat cgccaggtcg gcgagcgtcc actccaaccg ggtgtgggac
      481 atgatcgcaa tgctgtcacc cgcaccgtac ccggctgcct tgagaccctt cgcaagccct
      541 cgcacgacct ccagggtctt tgccgccgac cagtcgaccc agacgtcgcc gaccttctcg
      601 cggtacagcg tggtggccgg gctctgggtg ccgtgccaga tgaggtagtc gctgatcgac
      661 attcccgggt caacactcgc gaggcgcgca gcgtggattt cagccattcc gactcctttg
      721 tcgagtcgat tatcccgcag gtacgctcca ctgggcgcgg acgtcgaact gggtcgtgag
      781 cgcgtcggtg ggtggtttca cggcaggaca tcgaacagca agtcagcgag cccgaatcgt
      841 ggttccacca accggtggcc gaaggggctc acgtcgatgc gtctgcgcat cagagaccgg
      901 tagataccga tgctgcggat gttgccggcc aactgcgcac cggtgatccg gccgtcgcgg
      961 aggtagacgg agcgcagttg ctgtccgtcg ccccagcgga gcacggcgtc ggggttgtcg
     1021 gtcgagccga ccgcaacgat cggcacaccg aggtgcttga ggctgttcat cgcttcggcg
     1081 ccctcgtagg tcagctgcgc gccaagcagg ttggcggcgg cgatcggcgc ctgcctgacg
     1141 gcgttgtgga agatcgcgtg cacataacgc tcgccggtca tgtagtcgcg cgtcactgcc
     1201 acgtcaccag cggccaccac gccgggcgca gaggtccgca gcgagtcgtc caccagcacg
     1261 ccccaatcgg tttggatggc ggagtcggcg aggtggccga tgtgtggctt gacgccggtc
     1321 gccgccagca cgaggtcggc cgtgagggcg ctgccgtcgg ccaaccggac gccattggcg
     1381 ggatttccga tgaacgcggt cgcctccacg ccgagccgga gcgtgacccc gcgcgctcgc
     1441 acggccgcct ctgcgatggc ggaggtctcg cggtcgagtg cccggggcat gagccacgac
     1501 cggcggccga cgatcgtggt gtccactccg aggtccgcga gcagcagcga cagctccacc
     1561 ccgatgaagc cgttgcccac cacgagggcg gttcggcact cctgcgagcg cactcgggcc
     1621 agcagcgacc gggccgtgct cagggacttg aagtccagga tcccgggtag gtccgccccc
     1681 tcgagcggtg cgtgcagccg actgccggag gcgacgacga gcccctcata ggcgaggcgg
     1741 gcgccgtcgt cgagcacgac ctcacggttg tcggtgtcga ccccgcgcac gacggcgccg
     1801 cgccgctcct cgatgccgag gcggcgggcg gcgtcctgtc ccttccagaa cagcgggccc
     1861 tcgcgaccgg tcagcaggtg gtcggcgagc gcgaccggag agtacggcgg gaacggctcc
     1921 gcggacagag cggtgaccga gccgtgggga tcgagccgcc ggagcgtctc cgcggccgtg
     1981 atcccggcgg gcccggttcc gatcacgacg acgtgcatca cacctccaag agggcgggct
     2041 cgtgatggtg gtgatgctcg tgctggtggt gatgctcgtg cgggtgggcc gg
//
LOCUS       DASRKH010000051         2791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_50583, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000051 DASRKH010000000
VERSION     DASRKH010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50583"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="VFC06_00855"
     CDS             <1..942
                     /locus_tag="VFC06_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HZL80485.1"
                     /translation="LLGVGDDGSDEVGNAFVDAQALEWVVKEMDTRYDDLAMYGYKHI
                     DDFNKAVRAGKVKPLPGSERRITTYPYLLVIVDELADLMMVAPRDVDDSIQRITQLAR
                     AAGIHLVLATQRPSVDIVTGTIKANVPSRLAFATSSLADSRVVLDMPGAEKLIGQGDA
                     LFLPMGESKPMRVQGAWVTESEIHAAVEHVKTQAQPQFRDDVVTVSNQAKVAEEIGGD
                     MDELLAAAELVITTQLGSTSMLQRKLRVGFAKAGRLMDILESREVVGPSQGSKAREVF
                     VTPDELVSTLAILRGTSSDTVSDIITNDSEDGPWEDS"
     gene            927..>2791
                     /locus_tag="VFC06_00860"
     CDS             927..>2791
                     /locus_tag="VFC06_00860"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZL80486.1"
                     /translation="MGGLVEQLQQPNVAAGAAVLVLMGFVVAFFSFVRSGKARRTRDE
                     ALKRERSLRQQFDAILSSARDGVIIVSQTGEIAVLTEAAAQMIGVDRAAAVGKALGRL
                     PFKGVDEHMRPVMAHEAFAPGRDDLRPRVIGVPGRRGHEDLRWVQVMSHTVADAVDGE
                     PATITTIMDTTGLQEAAEALHHSDTQFRRAMENAPIGMGLVDLEWRLMEVNRAFAELM
                     GSTVAALRGTPFSALSHPHDLQAERDQLQRLYDGLQSHFALEKRYVRSDGNVVWAVLD
                     VALVRYAGGAPDHYVVQVRDSTDDRLTSELMAHRAMHDPLTGLANRTLMQDVLQRVLE
                     QSDATLNVGVIALDLDGFKGLNDRFGHATGDNALVHVAGVLRSATAGRGTVARVGGDE
                     FVIVVQDPDVSRALQDIASTIHVGLKRPLQVKRHQLNLHASVGIAIADSDTIEGGAPS
                     LLGAADAAMYRAKAMGKSRTEVYDPSMRVTNENQSALAGELVQGIERGDLVLHYQPVV
                     ELATRAVVGYEGLVRWQHPTRGLLLPGAFLGLLEDRNVATQLGANLVEQAAVFLTKCP
                     SPSAWVSVNVSADQLGDSEFANSVLGAIARHNLSPQRLVVELTEASLVAPNTRIR"
BASE COUNT          495 a          905 c          921 g          470 t
ORIGIN      
        1 cttcttgggg tcggtgatga tgggagtgat gaggtgggga atgccttcgt agacgctcag
       61 gccctcgaat gggtggtcaa ggagatggat accaggtacg acgatctggc gatgtacggg
      121 tacaagcaca tcgacgactt caacaaggca gtgcgcgccg gcaaggtcaa gccgttgccc
      181 ggctccgagc gccgcatcac cacctacccg tacctgctgg tgatcgtgga tgagttggct
      241 gacctcatga tggtcgcccc gcgtgacgtc gacgactcga tccagcgcat cacccaattg
      301 gcgcgcgcgg cgggcatcca cttggtgctc gcgacccagc gaccctcggt ggacatcgtc
      361 accggaacca tcaaggccaa cgtgccgtcg cgcctggcct tcgccacctc gtcgctcgcc
      421 gactcgcgcg tggtgctcga catgccgggc gccgagaagc tgatcggcca gggcgacgcc
      481 ctgttcctac ccatgggcga gtccaagccg atgcgcgttc aaggcgcctg ggtcaccgag
      541 tcggagattc acgccgcagt cgagcacgtg aagacccaag cacagcccca gttcagggac
      601 gacgtggtga cggtgagcaa tcaggccaag gtcgcggaag aaatcggcgg cgatatggac
      661 gagctgctgg ccgccgccga actggtcatc acgactcagc tggggtccac gtcgatgctg
      721 caacgcaaac tgcgcgtggg tttcgccaag gctggccgcc tcatggatat tctcgagagc
      781 cgcgaagtcg tcggcccgtc tcaaggctcc aaagcgcgtg aggtattcgt cactcccgat
      841 gagttggtat caacgctggc tattctgcgg ggcacttcct cggatacggt gagtgacatc
      901 atcaccaatg attccgagga cggaccgtgg gaggactcgt agagcagctc caacagccga
      961 acgtggctgc tggagcggcg gtgctcgtcc tcatgggatt tgtggtcgcg ttcttctcgt
     1021 tcgtgcggtc cggaaaggcc cggcgcacac gtgacgaggc cctcaagcgc gaaagaagcc
     1081 tgcgtcagca attcgacgcg atcttgtcct cggcccgcga cggcgtgatc atcgtctccc
     1141 aaaccggcga gatcgcggtg ttgaccgagg ccgctgcgca gatgatcggc gtcgacaggg
     1201 ccgcagcggt cggcaaggcc ttgggtcgcc tgcccttcaa gggcgtcgac gagcacatgc
     1261 gccccgtcat ggctcacgag gcctttgccc cagggcgcga cgaccttcgg ccccgcgtga
     1321 tcggcgtgcc aggcaggcgc ggccatgagg atcttcgctg ggttcaggtg atgtcgcaca
     1381 cggttgccga cgcggtggac ggcgagccag cgacgatcac cacgatcatg gacaccacag
     1441 gtctccagga ggccgcagag gcgctccacc actccgacac ccagttccga cgtgccatgg
     1501 agaacgcgcc cattggcatg gggctcgtgg acctcgagtg gcgtctcatg gaggtcaacc
     1561 gggcgtttgc cgagctcatg gggtccaccg tggcggcact gcgcggcacc cctttcagcg
     1621 ccctgtccca cccgcacgat ctgcaagcgg agcgcgacca gttgcagagg ttgtacgacg
     1681 ggcttcagag ccacttcgcg ctcgagaagc ggtatgtgcg ctccgacggc aacgtggtgt
     1741 gggccgtgtt ggacgtggcg ctcgtgcgct acgcgggcgg cgcgcccgac cattacgtgg
     1801 tgcaggtgcg cgactcgacg gacgaccgcc tcacctccga gctgatggcg caccgggcca
     1861 tgcacgaccc cctcacgggt ttggccaacc gcaccctgat gcaggacgtg cttcagcgag
     1921 tccttgaaca gtccgacgcg accctgaatg tcggcgtcat cgcgttggac ctcgacggct
     1981 tcaagggcct gaatgaccgc tttggccacg ccaccggcga caacgcgctg gtgcacgtcg
     2041 ccggagtgct gcgctccgcg acggcgggcc gcggcaccgt ggcgcgcgtt ggcggcgacg
     2101 agtttgtgat cgttgtgcaa gatcccgacg tgtccagggc actgcaggac atcgcgtcga
     2161 ccatccacgt cggcctcaag cgcccgcttc aggtcaagcg tcaccaactc aaccttcacg
     2221 catccgtcgg catcgccatc gcggacagcg acaccatcga gggcggtgcc cccagcctcc
     2281 tgggcgcggc cgacgcggcc atgtatcgcg ccaaagcgat gggcaagtcg cgcaccgagg
     2341 tgtacgaccc ctcgatgcgc gtcacaaacg agaaccagag cgcgttggcc ggagagttgg
     2401 tgcaagggat cgagcggggc gatttggtgc tccactacca accggtggtg gaactcgcga
     2461 cgcgcgccgt cgtcggctac gaggggctgg tgcgatggca acaccccacg cgcggcctgc
     2521 tgctccccgg ggcattcttg gggctgctgg aggatcgcaa cgtggcgacg caactgggag
     2581 ccaatttggt ggagcaggcc gcggtcttcc tcaccaagtg cccttcacca tcggcgtggg
     2641 tgtcagtcaa cgtctcggca gaccaactgg gtgacagcga attcgccaac tcggtgctgg
     2701 gcgcgatcgc gcgtcacaat ctcagcccgc aacgactcgt cgtggaactc accgaagcgt
     2761 cattggtggc cccgaacacg cgcatccgcc a
//
LOCUS       DASRKH010000052         2731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_46180, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000052 DASRKH010000000
VERSION     DASRKH010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46180"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1235)
                     /locus_tag="VFC06_00865"
     CDS             complement(<1..1235)
                     /locus_tag="VFC06_00865"
                     /inference="COORDINATES: protein motif:HMM:NF045328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80487.1"
                     /translation="MTASTLAGVLRGASTRIELSAAGDVRAIRHSDATLVSQYLATPH
                     DAMVGGVFLRRLTPAGTVESAALVGGRADGTFTVDDRCARWSGRALGAQYTVTLTLDP
                     DGVLWVWRVDLSAAPAEADPAADEGATYDVVLAQDLALAPEAMSTSNEPYVSQYVVHH
                     VIDDQRAGAVVASRQTMATAPVLPLALTAVVEGARAYLTDGFSFYGLGAKLDGHAAAL
                     DDASWESLTYQYEFAMPTLLGPSFDLTTPRTVHAVTAFVPDYRGELTDAPAAVPDLLD
                     RAVALAATTAPATSAGAGRHTADTDEPAAPGVRAPSVRSLLVSARLLAGRDLTADELV
                     GLTATAAGAANVLRPETAPDGTLLSYFTADATHVVSRAKELLVERPHGHVLKAGSDVT
                     PNVNVFSATTYAYGVFASHV"
     gene            complement(1232..2446)
                     /locus_tag="VFC06_00870"
     CDS             complement(1232..2446)
                     /locus_tag="VFC06_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HZL80488.1"
                     /translation="MLHFPEGFRWGSATAAHQIEGNNVNSDWWAREHALGTTIAEPSG
                     DACDSYHRYREDMQVLADVGLGTYRFSLEWARIEPEKGFVSRAELDHYRRMVDTAREL
                     GIEPMVTLHHFTIPRWFDADGGWFAPDAVDRFTRFTETVLPVLDGVELVCTINEPNMV
                     AMFAGDDPTNAEHTTLQAFGQPAPNPRVAETLLAAHRSARDVLAAAGPFRSGWTVATQ
                     DFQPEPGCEELARGYGYPRDAWYLEAARGDDWVGVQAYTRTKIGPDGPLPVPDDAEKT
                     LTGWEFYPRALEDGVRLAHAMAPGVPVFVTENGIATADDARRIDYTRAALTGLHAAIA
                     DGIPVEGYLCWSLLDNYEWGSYGPTFGLVAVDRETFVRTVKPSARWLGEVARANAVEA
                     TAVEEDPVEATA"
     gene            complement(2446..>2731)
                     /locus_tag="VFC06_00875"
     CDS             complement(2446..>2731)
                     /locus_tag="VFC06_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZL80489.1"
                     /translation="VLIADEPVSMLDVSLRAGVLALLDDLRVQFGVSMLYITHDLLSA
                     RLVTDHIMVLNSGRVVESGVTAQVLRFPQDAYTIELLDAIPQPARAGGAA"
BASE COUNT          366 a          946 c         1044 g          375 t
ORIGIN      
        1 acgtgcgacg cgaacactcc gtacgcgtat gtggtcgccg agaacacgtt gacattcggc
       61 gtcacgtcgg agccggcctt gagcacgtgg ccgtgcggac gctcgacgag gagctccttg
      121 gcccggctga cgacgtgggt cgcgtcggcg gtgaagtacg acagcagcgt cccgtcgggg
      181 gcggtctcgg gacgcagcac gttcgctgcg cccgctgccg tcgcggtgag gccgacgagc
      241 tcgtcggccg tcaggtcccg gcccgcgagc agccgggccg agacgagcag ggaccgcacg
      301 gacggtgccc ggaccccggg tgcagccggc tcgtcggtgt cggccgtgtg ccggcccgca
      361 ccggccgagg tcgccggcgc ggtcgtagcg gcgagcgcga ctgctcggtc gagcaggtcc
      421 ggcacggcgg cgggcgcgtc ggtgagctcg ccgcggtagt cgggcacgaa cgcggtcacg
      481 gcgtgcaccg tgcgcggggt cgtcaggtcg aacgacggcc cgagcagggt cggcatcgcg
      541 aactcgtact ggtaggtcag cgactcccag gacgcgtcgt cgagcgcggc ggcgtgcccg
      601 tcgagcttcg ctccgagccc gtagaaggag aacccgtcgg tgaggtaggc gcgtgcgccc
      661 tcgaccaccg cggtcagcgc gagggggagg acgggtgcgg tcgccatcgt ctggcggctc
      721 gcgacgactg ctcctgcgcg ctggtcgtcg atcacgtggt gcacgacgta ctggctgacg
      781 tacggctcgt tgctcgtgga catcgcctcg ggggcgagcg cgaggtcctg ggcgaggacg
      841 acgtcgtagg tcgcgccttc gtcggccgcg gggtccgctt cggcgggggc ggccgagagg
      901 tcgacgcgcc acacccagag caccccgtcc gggtcgagcg tcagcgtgac ggtgtactgc
      961 gcgccgaggg cccggcccga ccagcgcgcg cagcggtcgt cgaccgtgaa cgtgccgtcc
     1021 gcgcgtccgc ccacgagagc ggcggactcg accgtgcccg cgggcgtgag tcggcgcagg
     1081 aagacgccgc ccaccatcgc gtcgtgcggc gtggcgaggt actggctcac gagcgtggcg
     1141 tcgctgtggc ggatcgcccg gacgtcaccc gccgcggaca gctcgatgcg cgtgctcgcg
     1201 ccgcgcagca cgcccgcgag cgtcgacgcc gtcacgcggt ggcctcgacc gggtcttcct
     1261 cgaccgcggt ggcctcgacc gcgttggccc gcgcgacctc gccgagccag cgcgcgctcg
     1321 gcttgacggt gcgcacgaac gtctcgcggt cgaccgcgac gaggccgaac gtcggcccgt
     1381 acgagcccca ctcgtagttg tcgagcaggc tccagcagag gtagccctcg accgggatcc
     1441 cgtcggcgat cgccgcgtgc aggcccgtga gcgccgcgcg cgtgtagtcg atccggcggg
     1501 cgtcgtcggc ggtcgcgatg ccgttctcgg tgacgaagac ggggacgccg ggcgccatcg
     1561 cgtgggcgag gcggacaccg tcctcgagcg cacgggggta gaactcccac ccggtgagcg
     1621 tcttctcggc gtcgtccgga acgggcagcg ggccgtcggg cccgatcttc gtgcgcgtgt
     1681 acgcctgcac accgacccag tcgtcaccgc gcgcggcctc gaggtaccac gcgtcgcgcg
     1741 ggtagccgta gccgcgggcg agctcctcgc agccgggctc gggctggaag tcctgcgtcg
     1801 cgacggtcca tcccgagcgg aacggccccg cggcggcgag cacgtcacgg gcggaccggt
     1861 gggcggcgag cagcgtctcg gcgacgcgcg ggttcggcgc gggctggccg aacgcctgca
     1921 gcgtcgtgtg ctcggcgttc gtcgggtcgt cgcccgcgaa catcgcgacc atgttgggct
     1981 cgttgatcgt gcagaccagc tcgacgccgt cgagcacggg cagcacggtc tcggtgaacc
     2041 gcgtgaagcg gtcgaccgcg tcgggtgcga accagccgcc gtcggcgtcg aaccagcgcg
     2101 ggatcgtgaa gtggtgcagc gtgaccatcg gctcgatgcc gagctcgcgc gccgtgtcga
     2161 ccatgcgccg gtagtggtcg agctcggcgc gggacacgaa gcccttctcg ggctcgatcc
     2221 gggcccactc gagcgagaac cggtacgtgc cgaggccgac gtcggccagg acctgcatgt
     2281 cctcgcggta gcggtgatag ctgtcgcacg cgtcaccgct cggctccgcg atcgtggtgc
     2341 cgagcgcatg ctcgcgcgcc caccagtcgg agttgacgtt gttgccctcg atctggtggg
     2401 ccgctgtcgc ggacccccac cggaacccct cggggaagtg cagcatcagg cggcgcctcc
     2461 ggcccgcgcc ggctggggga tcgcgtcgag cagctcgatc gtgtacgcgt cctgcgggaa
     2521 gcgcaggacc tgcgcggtga cacccgactc cacgacccga ccgctgttga gcaccatgat
     2581 gtggtcggtc acgaggcgcg cggagagcag gtcgtgcgtg atgtacaaca tcgagacccc
     2641 gaactgcaca cgcaggtcgt cgagcagcgc gagcaccccg gcccggagcg agacgtcgag
     2701 catcgagacc ggctcgtcgg cgatgaggac c
//
LOCUS       DASRKH010000053         2414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_55111, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000053 DASRKH010000000
VERSION     DASRKH010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55111"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1365)
                     /locus_tag="VFC06_00880"
     CDS             complement(<1..1365)
                     /locus_tag="VFC06_00880"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HZL80490.1"
                     /translation="MTELPLNRPDGTHELPVPPTMVPRAARQLLDDGYRLALVAAHDD
                     GPAGFHLAYAFAHPGTDERRELVVRLPHEDPTVPSLAGLSFSAGRFERAVRDQFGIVP
                     VGHPRPRRLVLHDHWPHGWYPMRRDADPDPAFLPDEGTFSFLPVAGDGVYEIPVGPVH
                     AGMIEPGHFRFSVVGETIVRMKAQLWFLHRGIEKLFEGRTPEEGIELAERVSGDTAVG
                     HALTYAMAVEGALRTEVSEEDRLVRALLLELERLYNHVADIGAIANDVGFGIVNTHAQ
                     RLRETLLRHNKAVTGHRLLRGGITIGGSTLLTLPDPELIAGVAAEVADLVVIALGNTV
                     VADRFTGTAVLPRTEAETMGVLGYVARASGVAIDARADHPFVDLGDAFHVCTRDDGDV
                     LARFGVRADEVAVSAALVADLVRRLGGRTGSRRHLGGGTGRVVAASGGGTARPYAAKG
                     LALVE"
     gene            complement(1362..>2414)
                     /locus_tag="VFC06_00885"
     CDS             complement(1362..>2414)
                     /locus_tag="VFC06_00885"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HZL80491.1"
                     /translation="AFLVGHQGTRRSLEAAWKYVVLGSVGVAIALLGVVLLYAASRSA
                     GEATLSWVALASGELQLDPGLTKVAAGLAVLGFATKAGLAPMHSWLPDAHSQAPAPVS
                     GLMSGVLLAVAFYAILRIQAIADMVLGPSLMRGLLLTAGLLSLAVAAALILRQRDYKR
                     MLAYSSIEHMGLLAVGAAIGGPFAVAAVLLHVLGHGLAKATLFVVAGRILATEGSTRI
                     EDVRALLARHPRMAVPYIAGMAALLGFPPFSLFFSEVGIVMAGWRAGLGWAMGLTLAL
                     LLVLFAGLARASAAMVLGAPVSDAAPRHPMHPGHGSPEGPMTPLVLALAVTAVAGFLS
                     VPLGTVLLRAAAVLGS"
BASE COUNT          367 a          884 c          894 g          269 t
ORIGIN      
        1 ctccaccagc gcaagcccct tcgcggcata cggtctggcc gtcccgccgc ccgacgccgc
       61 cacaacgcgg cccgtgcccc cacccagatg cctccgcgac ccggtccggc caccgagccg
      121 ccgcaccagg tcggcgacga gcgccgcgga caccgccacc tcgtccgcgc gcacgccgaa
      181 gcgggccagg acgtcgccgt cgtcgcgcgt gcacacgtgg aaagcatcgc cgaggtcgac
      241 gaacgggtgg tcagcccgcg cgtcgatcgc gacgccggag gcccgggcca cgtagccgag
      301 cacgcccatg gtctcggcct cggtgcgggg caggacggcc gtgcccgtga accggtccgc
      361 gacgacggta ttgccgagcg caatgacgac gaggtcagcc acctcggcgg cgacccccgc
      421 gatgagctcg gggtcgggca gggtgagcag ggtggagccg ccgatggtga tcccgccgcg
      481 gagcagccgg tgcccggtca ccgccttgtt gtggcgcagt agggtctcgc gcagccgctg
      541 cgcgtgcgtg ttgacgatgc cgaacccgac gtcattggcg atcgcgccga tgtccgccac
      601 atggttgtac agccgctcaa gttccagcag cagcgccctg accaggcggt cctcctcact
      661 gacctcggtc cgcagggcgc cctccacggc catcgcgtag gtgagggcgt gcccgacggc
      721 ggtgtccccg ctgacccgct cggccaattc gatgccctcc tccggggtgc ggccctcgaa
      781 cagcttctcg atcccgcggt gcaggaacca cagctgcgcc ttcatccgca cgatcgtctc
      841 cccgaccacg gagaaccgga agtgcccggg ttcgatcatc ccggcgtgca ccggacccac
      901 cgggatctcg tacacgccgt cgcccgccac gggcaggaac gagaaggtgc cctcgtcggg
      961 caggaaggcg gggtccgggt ccgcgtcgcg gcgcatgggg taccagccgt ggggccagtg
     1021 gtcgtggagc acgagccgcc gcggccgcgg gtggccgacc gggacgatgc cgaactggtc
     1081 ccgcaccgcg cgctcgaacc gtcccgccga gaaggacagc ccggccaggg acggcaccgt
     1141 ggggtcctcg tgtgggagtc gcaccacgag ttcccggcgc tcgtccgtgc ccgggtgcgc
     1201 gaaggcgtac gccaggtgga agcccgccgg gccgtcgtcg tgcgcggcga ccagcgcgag
     1261 gcgatagccg tcgtcgagca gctggcgcgc cgcccgcggc accatggtgg gcgggactgg
     1321 cagctcgtgc gtcccgtccg gcctgttcag gggcagctca gtcatgaacc cagcaccgcc
     1381 gcggctcgga ggagcaccgt gccgagcggc acggagagga atcccgccac cgcggtcacg
     1441 gccagcgcca ggacgagggg cgtcatcggg ccctcgggcg agccgtggcc ggggtgcatg
     1501 gggtggcggg gcgcggcgtc ggacacgggc gcgcccagca ccatcgcggc gctcgcccgg
     1561 gcgaggccgg cgaacagcac gaggagcagg gcgagcgtga gccccatagc ccagccgagg
     1621 cccgcccgcc agccggccat cacgatcccc acctcggaga agaacagcga gaacggcggg
     1681 aagccgagca acgccgccat tcccgcgatg tatgggacgg ccatccgcgg gtgccgggcg
     1741 aggagggcac ggacgtcctc gatccgggtg ctgccctccg tggcgaggat gcgccccgcc
     1801 accacgaaca gcgtggcctt ggcgagcccg tgccccagca cgtgcagcag gacggcggcg
     1861 acggcgaagg gcccgccgat cgcggcgccg acggcgagca ggcccatgtg ctcgatgctc
     1921 gagtaggcga gcatgcgctt gtagtcgcgc tggcgcagga tgagcgccgc ggcgacggcg
     1981 agcgagagca ggcctgcagt gagcagcagc ccccgcatga gggatggccc gagcaccatg
     2041 tcggcgatcg cctggatccg caggatcgcg tagaacgcca ccgcgaggag caccccggac
     2101 atgagcccgg agaccggggc gggcgcctgg ctgtgcgcgt ccgggagcca ggagtgcatc
     2161 ggtgcgaggc ccgccttggt ggcgaagccg agcacggcca ggccggccgc caccttcgtg
     2221 aggcccgggt ccagctgcag ctcaccgctg gcgagggcga cccacgaaag ggtcgcctcc
     2281 ccggcgctgc ggctggcggc gtagagcagg acgacgccga gcagcgcgat ggccaccccg
     2341 acggagccga gcaccacgta cttccacgcc gcctccagcg agcggcgcgt gccctggtgg
     2401 cccaccagga aggc
//
LOCUS       DASRKH010000054         2035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_50522, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000054 DASRKH010000000
VERSION     DASRKH010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50522"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="VFC06_00890"
     CDS             complement(<1..694)
                     /locus_tag="VFC06_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006943376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HZL80492.1"
                     /translation="MSDDYDDDYQADAEYLKNYVPPAPSDPQESLDEVRQAALRAGLE
                     AYELDERDLATLDGEPTEQREALPILAIVGRPNVGKSTLINRIIGRRLAVVEDTPGVT
                     RDRVTYEAEWAGRDFMLMDTGGWEAGVHGIDLSVAQSAEVAMELADVVLFVVDATVGP
                     TATDEKVVKLLRKTKKPVVLVANKVDGPQGELEAASLLNRLADSERVVVHDVAGTTRS
                     ESASRFSSEDLPT"
     gene            complement(691..1350)
                     /gene="cmk"
                     /locus_tag="VFC06_00895"
     CDS             complement(691..1350)
                     /gene="cmk"
                     /locus_tag="VFC06_00895"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HZL80493.1"
                     /translation="MSIVIAIDGAAGTGKSTVSREVARRLGLEYLDTGATYRVGALYC
                     LREGIDLKNQDDVTAMIATMNVEMRLDPDNPHVILDGDDVSNLLHTDTISLVVSAVAT
                     NLDARAVLRDVQRKLIDASRNRAGVVAEGRDITTVVAPDAEVRVLLTADEEARIARRT
                     GDGADPATVRESVLGRDRKDSTVVQFHTAADGVVEIDTTLLTLEEVVGAVLTLAKEAG
                     A"
     gene            complement(1347..>2035)
                     /locus_tag="VFC06_00900"
     CDS             complement(1347..>2035)
                     /locus_tag="VFC06_00900"
                     /inference="COORDINATES: protein motif:HMM:NF042869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prephenate dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HZL80494.1"
                     /translation="MAAQGDLFRARPWVLCAGQASAAAVALVTQVADALQCDIIEMDA
                     HAHDRAVARVSHAPQAAASAVAAALAPLGVDDVALAGQGLRDVTRIAASRPEMWADIA
                     RLNRQSIVETIDHIVEELDAVRSAEDIGEAMTRLIDKGRQEVARIPGKHGGAKREWEA
                     VTVIVPDEPGRLVELLSDAAAVGVNVEDIAIEHSPRQPVGLTTLWVLPQHAASLVIAL
                     EDGGWTVAGA"
BASE COUNT          298 a          723 c          668 g          346 t
ORIGIN      
        1 atgttggcaa gtcctcgctg ctgaaccggc tcgcggattc cgagcgcgtg gtgcccgcga
       61 cgtcgtgaac caccacgcgc tcggaatccg cgagccggtt cagcagcgag gcagcctcga
      121 gctctccctg ggggccgtcg accttattgg cgacgagcac cacgggcttc ttggtcttgc
      181 gcagcagctt gacgaccttc tcgtccgtcg cggtgggccc caccgtcgcg tcgaccacaa
      241 agagcaccac gtcggcaagc tccatggcca cctcggcaga ttgcgcgacg gacaggtcga
      301 tgccgtgcac acccgcttcc cagccgcccg tgtccatgag catgaagtcg cggccagccc
      361 actcggcctc gtacgtcacg cggtcccgag tgactccggg ggtgtcctcc acgacggcca
      421 ggcgacggcc gatgatgcgg ttgatcagcg tcgacttgcc cacattcggg cgacccacga
      481 tcgcgaggat cggcaacgcc tcgcgctgct ccgtcggctc gccgtccagc gtggcgaggt
      541 cgcgctcgtc gagctcgtag gcctcgaggc cagcgcgcag cgcggcctga cgcacctcgt
      601 cgaggctctc ctgaggatcc gagggcgcgg gtgggacgta gttcttcagg tactcggcgt
      661 cggcttggta gtcgtcgtcg tagtcgtcgc tcatgcgcct gcctccttgg cgagggtcag
      721 gacagcgccg actacctctt cgagcgtgag gagggtcgta tcaatctcca ccacgccatc
      781 ggccgccgtg tggaactgca ccacggtgga atccttcctg tctcgcccca gcacggactc
      841 gcgcacggtg gccggatcgg cgccgtcgcc ggtgcggcgg gcgatgcgcg cttcctcgtc
      901 ggcggtgagc aacacccgca cctcggcgtc cggcgccacg acggtcgtga tgtcgcgacc
      961 ctcggccacc acgccggccc ggttgcgcga cgcatcgatc agcttgcgct gcacgtcgcg
     1021 aagcactgcg cgcgcgtcga ggttggtggc caccgcagac accaccaggg agatggtgtc
     1081 ggtgtgcagc agattcgaga cgtcgtcgcc gtcaaggatg acgtgcgggt tgtccggatc
     1141 gaggcgcatc tcgacattca tggtggcgat catggccgtc acgtcgtcct ggttcttgag
     1201 gtcgattccc tcacgaaggc agtacagcgc gcccacgcgg tacgtcgcac cggtgtccag
     1261 gtactccagg ccaagcctgc gtgccacctc gcgcgacacc gtcgacttgc cggtgcctgc
     1321 tgctccgtca atcgcaatca cgatactcat gcgcctgcca ccgtccatcc gccgtcttcg
     1381 agggcaataa ccaaactggc cgcgtgctga ggaagcaccc acagcgtcgt cagcccgacg
     1441 ggctggcgcg gcgagtgctc aatcgcaatg tcctcgacgt tgacccctac ggcggccgcg
     1501 tcggacagca gctccaccag acgccccggc tcgtccggca cgatcacggt aacggcctcc
     1561 cactcgcgct ttgccccgcc gtgcttgccg gggatgcggg ctacctcttg gcgtcccttg
     1621 tcgatgaggc gcgtcatggc ctcgccgatg tcctcggcgg agcgcaccgc gtccagttcc
     1681 tcgacgatgt ggtcgatcgt ctcgacgatt gactgccggt tgaggcgggc gatgtcggcc
     1741 cacatttcgg gccgcgaggc ggcgatgcgc gtgacatcgc gcaggccctg tcccgcaagc
     1801 gcgacgtcgt ccacgccgag cggcgccagc gcggccgcga ccgccgatgc ggcggcttga
     1861 ggcgcgtgcg agacccttgc gacggcgcgg tcgtgcgcgt gtgcgtccat ctcgatgatg
     1921 tcgcactgca aggcatcggc cacttgggtc accaaagcca ccgccgcggc gctcgcctga
     1981 ccggcgcaca gcacccaggg ccgggccctg aagaggtctc cttgggcggc catcc
//
LOCUS       DASRKH010000055         2438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_45633, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000055 DASRKH010000000
VERSION     DASRKH010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45633"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /gene="map"
                     /locus_tag="VFC06_00905"
     CDS             complement(<1..669)
                     /gene="map"
                     /locus_tag="VFC06_00905"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009647818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HZL80495.1"
                     /translation="MLADRAPRGTLKPGNVSPKREVPRSIPRPEYVGRTGPERAHAPE
                     VKSAETIERIRVASRIAAQALVEAGKVVAPGVTTDEIDRIGHEYLCDHGAYPSSLDYM
                     GFPKSLCTSINEVICHGIPDDTVLAEGDIVNIDITAYMDGVHGDTNATFAVGAVDEES
                     RLLIERTRIAMERGIRAVKPGREVNVIGRVIEAYARRFDYGVVRDFTGHGVGEAFHSG
                     LIIPH"
     gene            704..919
                     /locus_tag="VFC06_00910"
     CDS             704..919
                     /locus_tag="VFC06_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015775696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HZL80496.1"
                     /translation="MTEYFYNLETGQVEEGRMSEGLELMGPYPTREAAANALGHARQR
                     NAEWDEADRAWKDDDDEWGTNPAPKRP"
     gene            complement(980..1816)
                     /gene="panB"
                     /locus_tag="VFC06_00915"
     CDS             complement(980..1816)
                     /gene="panB"
                     /locus_tag="VFC06_00915"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020014258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HZL80497.1"
                     /translation="MADNAPAKGVPVPQRVRVHHLRQAKERREPLTMLTAYDAVCARI
                     FDEAGIDLLLVGDSIGDNMLGHPSTIPVTVDEMVVAARAVASAARRAFVVADLPFGSY
                     EASPAQCHATAVRMIKEGLAHAVKFEGGAHLTGHVELCTRSGIPVFGHLGFTPQSENM
                     LGGKRVQGRGNVAAEKLVSDAKALQDAGAVAVVLEMVPAPVAARVTEVLEIPTIGIGA
                     GPDVDGQVLVWTDMAGMGSWSPRFAKQFGNLGAELYAATRRYSSAVRERQFPGPEHTY
                     ES"
     gene            1998..>2438
                     /locus_tag="VFC06_00920"
     CDS             1998..>2438
                     /locus_tag="VFC06_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015771764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HZL80498.1"
                     /translation="MDRQQEYVLRVVEERDIRFIQLWFTDVLGVLKSVAIAPAELEGA
                     FAEGIGFDGSAIEGLARVSEADMIAQPDPSTFQILPWRGEQHGTARMFCDILTPAGEA
                     ARTEPRAVLKRMLSRAAEKGFTFYTHPEIEFYLFEDPGESSELVP"
BASE COUNT          378 a          830 c          859 g          371 t
ORIGIN      
        1 gtgcgggatg atcaggcccg agtggaacgc ctcgcccaca ccgtggccgg tgaagtcccg
       61 caccacgccg tagtcgaagc ggcgcgcgta ggcctcgatc acgcggccga tgacgttgac
      121 ctcacggccg ggcttcacgg cccggatccc ccgctccatc gcgatgcggg tgcgctcgat
      181 gagcagccgg gactcctcgt ccaccgcacc gacggcgaac gtggcgttcg tgtccccatg
      241 cacgccgtcc atgtaggcgg tgatgtcgat gttgacgatg tcgccctccg ccagcacggt
      301 gtcgtccggg ataccgtggc agatcacctc gttgatcgag gtgcacagcg acttcgggaa
      361 ccccatgtag tccagcgagg aggggtaggc gccgtggtca cagagatact cgtggccgat
      421 ccggtcgatc tcatcggtgg tgacgccggg cgccacgacc ttgccggcct ccacgagcgc
      481 ttgcgccgct atccggctcg ccacgcggat ccgctcgatc gtctcggcgg acttcacctc
      541 gggggcgtgc gcgcgctcgg gacccgtgcg gccgacgtat tccgggcggg ggatggatct
      601 cgggacttcg cgcttaggtg agacgtttcc gggtttcagg gtgcctcggg gcgcgcgatc
      661 agccaacatg gtattgagtt tacgttcccg ggaggagcac gagatgaccg agtacttcta
      721 caacctcgaa acggggcagg tggaggaggg ccggatgtca gaggggctgg aactcatggg
      781 cccgtacccg actcgtgagg ccgccgccaa cgccctcggc cacgcccgcc agcgcaatgc
      841 cgagtgggac gaggccgacc gcgcgtggaa agacgacgac gacgagtggg gcacgaatcc
      901 ggcgccgaaa cgcccctgag ccccgaactc cgctcgtgcg gcgtcgtcgc tcgagtccgc
      961 cacgaccggt cggcgcgcgc tacgactcgt aggtgtgctc cgggccgggg aactgccgct
     1021 cccgcactgc tgaggagtag cgcctcgtcg cggcgtacag ctcggcgccg aggttgccga
     1081 actgcttcgc gaaccgcggc gaccaggatc ccatgccggc catgtccgtc cacaccagca
     1141 cctggccgtc gacatcgggc ccggcgccga tcccgatcgt cgggatctcc agcacctcgg
     1201 tgacccgcgc ggccaccggc gccgggacca tctccagcac caccgcgacg gcgcccgcgt
     1261 cctgcagcgc cttcgcgtcg gacaccagct tctccgcggc cacgttcccc ctgccctgga
     1321 cccgcttgcc gccgagcatg ttctcggact ggggtgtgaa cccgaggtgg ccgaagacgg
     1381 ggatccccga gcgggtgcac aactccacgt gacctgtgag gtgggcgccc ccctcgaact
     1441 tcacggcgtg ggccaggccc tccttgatca tccgcacggc ggtggcgtgg cactgcgcgg
     1501 gggaggcctc gtagctcccg aagggcaggt ccgccaccac gaacgcgcgg cgggccgcac
     1561 tcgcgacggc ccgcgcggcg accaccatct cgtccacggt gacggggatg gtggacgggt
     1621 ggcccagcat gttgtcgccg atcgagtcgc cgacgagcag caggtcgatc cccgcctcgt
     1681 cgaagatgcg ggcgcacacc gcgtcgtacg cggtcaacat ggtgagcggc tcgcggcgct
     1741 ccttggcctg gcgcaggtgg tggacgcgta cccgttgcgg aacggggaca cccttcgcgg
     1801 gagcgttgtc tgccacggtc gatcctctcc atcagcgagc cgcacacggc caggtccacg
     1861 atcgggtacc cgcagggacc agagtacgcg ccgggccgtg taacaggctg ttaacagggg
     1921 ggaggggtcg gcggactgcg gagggggcgc cggatgggcg atgatgggag gacaggcatc
     1981 gcggtcaagg agtgttcatg gatcggcaac aggagtacgt gctgcgcgtc gtcgaggagc
     2041 gcgatatccg cttcatccag ctctggttca ccgacgtgct cggtgtgttg aagtcggtgg
     2101 ccatcgcgcc cgccgaactc gagggggcat tcgccgaggg catcggcttc gacggctccg
     2161 cgatcgaggg cctcgcgcgc gtctcggagg cggacatgat cgcccaaccg gacccgtcca
     2221 cgttccagat cctgccgtgg cgtggcgagc agcacgggac ggcgcgcatg ttctgcgaca
     2281 tcctcacccc cgcgggagag gcggccagga ccgagccgcg cgcggtgctg aagcggatgc
     2341 tctcgagggc ggcggagaag ggcttcacct tctacaccca ccccgagatc gagttctacc
     2401 tgttcgagga tccgggcgag agcagcgagc tggtcccc
//
LOCUS       DASRKH010000056         3021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_15432, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000056 DASRKH010000000
VERSION     DASRKH010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15432"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2113
                     /locus_tag="VFC06_00925"
     CDS             <1..2113
                     /locus_tag="VFC06_00925"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HZL80499.1"
                     /translation="AHHGPRGEETGRAEPLRAAPGVIATRHVTADDEARAVVGFIKAR
                     RAELGHSDASPVTAAVLCRRRAQYDAITDACVAAGLDYEVVGLGGLLDVPDVADLLAL
                     LQVAHDPSRGDSLMRLVAGERLALGPRDLAALHDRAEELAGPRSEREGTASIVDALAA
                     LPRADWVSPRGRSLTAAALSRLGALANVVDAIRRHTYLPLPELVAFAERAWGLDIESA
                     VARRATRARRSVDAFTDSARTFQAGAERATLGAFLAWLDAARAEENGLDAPVREPDAA
                     AVQILTVHGAKGLEWDMVAVPGLNDGHFPSVGVPSASNPDYTDSGWLDGVGNVPYELR
                     LDRDQLPQWRFHGASDHKELAATIADFKREAGGYRLDEERRLFYVALTRARSHVLLSG
                     SWYSGGKRPRDPSPYVMELLKQGSVAAGQWDDLPDDDAPPEIERPPGLWPRVATPPQA
                     LRRELAASVVEAMEGLAEGHPRAGGTDASGATWDPDLPLAREISAMLAERADRTRGKG
                     QIELPSHVSTSALVAMSRDRAAFAAQLRRPVPVEPTRAAQRGSALHAWIEARYGHVPL
                     WEDEDADDDAAAELDALKATFLASEWASRTPTHVEADVELPVGSVTVRSRIDAVFGPG
                     AGLDRVTVVDWKSGVPPRDEAERAAREVQLAMYRLAWSARTGMPVDQIDAAFYYVAND
                     ATVRPERLLDRQEIEALLAGG"
     gene            2243..>3021
                     /locus_tag="VFC06_00930"
     CDS             2243..>3021
                     /locus_tag="VFC06_00930"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HZL80500.1"
                     /translation="MVMLLLSIANIASIVVAAGIGVRVLRGNRFTLSGLAVAIVLVAT
                     VMWSVISLVGAQEHFTEGSDLPERALFWSAVLIAGVRSLVRVLENPAWSPRPRDVVNL
                     IAHPAAMGVIAAIPQLHFLLVTADDGGNLSYAFGFWIHSAVGLALSVRPLTVLLDPRS
                     RIPRDSARTRFVMIVSWALPAAGYLISALVWGPTGPNLAPAFLIVPVAMMGSAVVRDG
                     LVDRLPLARGEVFEALASAVFVTDSLGRVIDVNAAARSLAR"
BASE COUNT          429 a         1020 c         1078 g          494 t
ORIGIN      
        1 cgcccatcac gggccccgcg gcgaggagac cggacgggcg gaaccgctcc gtgccgcgcc
       61 cggagtcatc gccacccggc acgtcaccgc ggacgacgag gcgcgcgccg tggtgggctt
      121 catcaaggcc aggcgcgccg aactgggaca ctccgacgcg tcgccggtga cggccgcagt
      181 gctgtgccgc cgccgcgccc agtacgacgc gatcaccgac gcgtgcgtgg ccgccggact
      241 cgactacgag gtggtgggcc tcggcgggct actggatgtg cccgacgtgg ccgatctgct
      301 cgccttgctt caggtggccc acgatccgtc gcgcggtgac tcgctcatgc gcctcgtcgc
      361 gggcgagcgg ctcgcgctgg gcccgcggga cctcgcggcc ctgcacgacc gtgccgagga
      421 actcgccggc cctcgctccg agcgcgaagg caccgcgagc atcgtcgacg cccttgccgc
      481 gttgccgcgc gccgactggg tgtcgccacg aggtcggtct ctcaccgcag ccgctctgtc
      541 gcgcttgggc gcgctggcga acgtggtcga cgcgatccgc cgacacacgt acttgccgct
      601 gcccgaactg gtggcattcg cggaacgcgc ctggggcctc gacatcgaat ccgccgtcgc
      661 gcgccgggcg acacgggcgc gacgatcggt ggacgccttc accgactccg ccaggacatt
      721 ccaggcgggg gcggagcgcg cgactttggg tgcgttcctc gcgtggctcg atgcggcccg
      781 cgccgaagag aacggattgg atgccccagt gcgtgaaccc gacgctgcgg ctgtgcagat
      841 tctgacggtg cacggggcca agggtctcga gtgggacatg gtggccgtgc caggcctcaa
      901 cgacggccac ttcccctcgg tgggcgtgcc gtccgcgagt aaccccgact acacggatag
      961 cggctggctc gacggcgtgg gcaacgtccc ctacgagttg cggctcgacc gcgaccagct
     1021 acctcaatgg cgcttccacg gtgcgagcga ccacaaggaa ctcgccgcca cgattgccga
     1081 cttcaagcgg gaggccggcg gctaccggct cgacgaggag cggcgcctgt tctatgtggc
     1141 gctcactcgg gcacgctcgc acgtgttgct gagcggatct tggtactccg ggggcaagcg
     1201 acctcgcgat ccgtcgccgt acgtgatgga gttgctgaag cagggcagcg tcgccgcggg
     1261 ccaatgggac gatctgcccg acgacgatgc tccccccgag atcgagcgtc cgcccggcct
     1321 gtggccacgc gtcgcaacgc cgccgcaagc gctacgacgt gaactcgccg cgtccgtggt
     1381 cgaggccatg gaaggcctcg ccgaagggca tcctcgagcg ggtgggaccg acgcgtccgg
     1441 cgcgacgtgg gacccggacc ttccgcttgc ccgcgagatt tcggccatgc tcgcggagcg
     1501 tgccgaccgc acccgcggca agggtcagat cgagttgccc tcgcacgtgt cgacgtccgc
     1561 tctcgtggcc atgagtcgtg atcgcgcggc cttcgccgcc caactgcgca ggcccgtccc
     1621 ggtggaaccg acgcgcgccg cccaacgggg atcggcgctg cacgcttgga tcgaggcgcg
     1681 ttatgggcac gtcccgctgt gggaagacga ggatgcggac gacgatgcgg cggccgagct
     1741 ggatgccctc aaggccacct tcctggcctc agagtgggcc tcccgtaccc ccacgcacgt
     1801 ggaggcggac gtggagttgc cggtgggcag tgtgacggtg cgctcgcgga tcgacgcggt
     1861 gttcggaccc ggagcggggc tcgatcgagt gacggtcgtg gattggaagt ccggcgtgcc
     1921 accgcgcgac gaggcggaga gggccgcccg ggaggtccaa ctcgcgatgt accggctcgc
     1981 ctggtcggcg cgcacgggaa tgccggtcga ccagatagac gcggcgttct actacgtggc
     2041 caacgacgcg acggtgcgtc cggagcgatt gctcgaccga caggagattg aggcgctcct
     2101 tgcgggaggc tagcgacgcc agccgtcagg cgtcggcgtt cacggcgtac ctggcagttg
     2161 agccgtcgtc cctacactgg agccaatgcg gtcgatcagg agaccgagag atgatgggtc
     2221 tggtcggacg tagggagagc ccatggtgat gctgctcctg tcgatcgcga acatcgcctc
     2281 gatcgtggtc gccgcaggga tcggtgtgcg tgtgctgcgc ggcaaccgct tcacgctgtc
     2341 cggacttgcc gtcgcgatcg tgctggtcgc caccgtgatg tggtccgtga tttctcttgt
     2401 cggggcgcag gaacacttca cggaaggcag cgatcttccg gagcgagcct tgttctggag
     2461 cgccgtgctg attgcgggag tccgcagcct ggtgcgggtg ttggagaacc ccgcgtggtc
     2521 gccgcggccg cgcgacgtcg tcaacctcat cgcccacccc gcggcgatgg gagttatcgc
     2581 cgcgatccca cagctccact tcttgctggt cactgccgac gacgggggca acctctcgta
     2641 cgcgttcgga ttctggatcc actcggcggt gggtctcgca ctgtcggtga ggccgctcac
     2701 cgtgttgctg gacccgcgca gccgcattcc ccgtgattcg gctcggacgc ggtttgtgat
     2761 gatcgtttcc tgggccttgc cggcggccgg ctacctgatt tctgcgctgg tgtggggccc
     2821 gaccgggccc aatctggccc ccgcattcct catagtcccg gtcgccatga tgggctccgc
     2881 cgtcgtcagg gacgggcttg tcgacaggct cccgctggcg aggggcgaag tattcgaggc
     2941 actcgcgagc gccgtcttcg tcaccgacag cctgggaagg gtgattgacg tcaacgcggc
     3001 ggcaagatcc cttgctcggg a
//
LOCUS       DASRKH010000057         3060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_15988, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000057 DASRKH010000000
VERSION     DASRKH010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15988"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..687)
                     /locus_tag="VFC06_00935"
     CDS             complement(217..687)
                     /locus_tag="VFC06_00935"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HZL80501.1"
                     /translation="MAESSAKRTNELKRHIALVAHDNKKVDLLEWASFNKGTLSKHHL
                     YATGTTGTMLEYELGLKVTCFLSGPVGGDQQIGAKIAEGVITVLIFFWDPLEPQPHDP
                     DVKALLRIATVWNVPMASNVATADLMISSPLLSSGYQSRRPDMAARPWINPTEA"
     gene            complement(715..927)
                     /locus_tag="VFC06_00940"
     CDS             complement(715..927)
                     /locus_tag="VFC06_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80502.1"
                     /translation="MGESSHPESGFRRMSLLAHFRLRSSLERFVDAEVSHGAAQQIAE
                     HLQPCWSCSSEVHTLRLIKATLARLA"
     gene            971..1594
                     /locus_tag="VFC06_00945"
     CDS             971..1594
                     /locus_tag="VFC06_00945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZL80503.1"
                     /translation="MINASPTADPSAFDKYVVPEIPVLYRVALSLAGQPADAEDLVQD
                     TLIRAYRAIDRFDGAYPRAWLLTILRHTHLNRVRVRSAVLLADGDGVAATMDRLGTAR
                     PAAEEVVVSELFEAVVADAMSALPEKHRQVIELVDIEGLSYQEAADTLGVPRGTVMSR
                     LHRARTRIRTPGSQRRGSRPVRRACDVETLALRLPPGGQGLAKLPRR"
     gene            1617..1787
                     /locus_tag="VFC06_00950"
     CDS             1617..1787
                     /locus_tag="VFC06_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80504.1"
                     /translation="MVAEHLEVCRKCGLEASTYLAIKTAIAANAPETTPVDNDAVARL
                     RDFADGLGDSKD"
     gene            complement(1815..2048)
                     /locus_tag="VFC06_00955"
     CDS             complement(1815..2048)
                     /locus_tag="VFC06_00955"
                     /inference="COORDINATES: protein motif:HMM:NF025510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HZL80505.1"
                     /translation="MIRTLVLDYEHVHIAFGVAGNLLFVVGSVLFYKAFDQYYALAVS
                     LFVVGSAFMLAGSLGSGLRRLLEHREQESSSRA"
     gene            2363..>3060
                     /locus_tag="VFC06_00960"
     CDS             2363..>3060
                     /locus_tag="VFC06_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HZL80506.1"
                     /translation="MTEHDFSDLRAVFVNCTLKPSPELSHTAGLMAVSTAIMRKNGVT
                     VDEIRAVDYDLAPGVYPDMTEHGAERDDWPELVPRILGADILVIGTPIWLGDKSSVCN
                     RVIERLYSLSGMLNDAGQYLYYGRVGGVVITGNEDGAKHVAMNVLYSLQHLGYAIPPQ
                     ADAGWIGPAGPGPSYLDEGSGGPQNDFTQRNTTFMTWNLMHLAAALKRGGGFPAHGNQ
                     RSEWDAGARFDHPNP"
BASE COUNT          546 a          957 c         1026 g          531 t
ORIGIN      
        1 cacgcggttc ggcgagggac cgcctcctcg cggcagcagc ggagtgcttc tacaaccatg
       61 gcgtgaacgg gaccgggatc gacgccaccg acctgctcgt cgacgggggt gtcatcgcag
      121 ctctgcgctc gggcgctctg actgttcccg gctcttgacg ctcggcggct ttgccgccgt
      181 ggccagcgcg attcagcacg ggttgtggtg ggacgctcag gcctccgtgg ggttgatcca
      241 gggtcgagcc gccatgtctg ggcggcggct ctggtagccg ctggacagca gggggctgga
      301 gatcatcagg tcggctgtgg cgacgttgga ggccatgggc acgttccaga ccgtggcgat
      361 ccgcaggagc gccttgacgt cggggtcgtg tggttggggt tcgagggggt cccagaagaa
      421 gataagcacc gtgatgacgc cctcggcgat cttggcgccg atctgctggt cgccgccgac
      481 ggggcccgag agaaagcagg tgaccttcag gcccagctca tactcgagca tcgttcccgt
      541 ggtcccggtc gcatacaggt gatgcttgga cagcgtgccc ttgttgaagc tcgcccactc
      601 gagcaggtcg accttcttgt tgtcgtgcgc cacgagcgcg atgtggcgtt tcagttcatt
      661 ggtgcgcttc gcactggatt cggccatggg tccagtatcg cgcctcggtg acggtcaggc
      721 taggcgcgct agcgtcgcct tgatcagtcg aagcgtgtgg acctcgctgc tgcacgacca
      781 gcacggctgc aggtgctcgg cgatctgctg cgctgcgccg tggctcactt cggcgtcgac
      841 aaaccgctca agactcgacc gaagtcgaaa atgggcgagc agggacatgc gacggaaacc
      901 cgactcgggg tgagacgatt cccccaaggg tccccatgga atacccaccg cagacgaggg
      961 gtttcgcaac gtgatcaacg ccagcccgac cgcagacccc tctgcgttcg acaagtacgt
     1021 ggtgccggag atcccggtgc tctaccgggt ggcgttgtcc ttggctggtc aaccggccga
     1081 cgccgaagac ctggtgcaag acacgctgat ccgcgcatac cgtgccatcg atcgttttga
     1141 cggggcatac ccgcgcgcct ggttgctcac gatcctgcgg cacacgcacc tcaatcgagt
     1201 gcgcgtgcga agtgcggtcc tgctcgccga cggtgacgga gtggcggcca ccatggaccg
     1261 acttggcacg gcgaggcccg cggccgagga ggttgtggtc agcgagttgt ttgaggcggt
     1321 cgttgctgac gcgatgtccg cgttgccgga aaagcatcgc caggtgatcg agctggtgga
     1381 catcgaagga ttgtcctacc aggaggcggc ggacacgcta ggtgtccctc gcggcaccgt
     1441 gatgagcagg ctgcaccggg cgcggacaag gatccgcacc cccggctcac agcggcgggg
     1501 ctcgcgcccc gtaagaaggg catgtgatgt tgaaactctc gcgctccgat tgccaccagg
     1561 tggccaaggt cttgcaaagc tacctcgacg gtgagactga ggcgcgtacg tcccggatgg
     1621 tggcggagca tctggaggtg tgccgtaagt gtgggctgga ggcctccacc tacctcgcga
     1681 tcaagacggc catcgccgcg aatgcccctg agacgactcc agtagacaac gacgccgtcg
     1741 caaggctgcg tgacttcgcc gatggattgg gcgattcgaa ggactgacac acccgatctg
     1801 tcgcgagact cccgtcacgc gcgggaagaa gactcttgct cgcggtgctc cagaagccgg
     1861 cgcaggccgc taccgagaga gccggccaac atgaaggcag agccgacgac gaacagcgac
     1921 accgcgagcg cgtagtactg atcgaaggcc ttgtagaaca gcaccgaacc cacgacgaac
     1981 aggaggttcc cggcgacgcc gaaggcgata tggacgtgct cataatccag gacgagcgtg
     2041 cggatcaaga tgagatccct cccagggtgc tgtcggtcgt ggtggcggag ccctgcatca
     2101 cgcggctcgc cgccgatctg aacgcgaacg taccatctcg cctacaagcc atcaccggca
     2161 gtccgtgttt caggcggctc tgaagccgac cggcggggcc gtccgtgcgg tagacgacga
     2221 tctcggagga cggatcgagt cctagttctc ccaccacgtc tctcagcgcc tcggtaccga
     2281 cgaacctccc gtcgctcgtc cgcaccatgg ataatggaaa gtggatcgtt cccgggatcc
     2341 actcgatgag gagcacttca ctatgacgga gcacgacttc tccgaccttc gcgccgtgtt
     2401 cgtcaactgc acgctgaagc cctcgccgga gctctcccac accgctggac tcatggcagt
     2461 cagcacggcg atcatgcgca agaacggcgt caccgtcgat gagattcgcg ccgtcgacta
     2521 tgaccttgcg cccggcgtct acccggacat gaccgagcac ggcgcggagc gcgacgactg
     2581 gccggagctg gtgccgcgaa tcctgggggc cgacattttg gtgatcggca ctcccatctg
     2641 gctcggcgac aagtcgagcg tgtgcaaccg agtgattgag cggctctatt cgttgtcggg
     2701 gatgctcaac gacgcgggcc agtacctgta ttacggcagg gtcggcgggg ttgtcatcac
     2761 gggcaacgag gacggggcga agcatgtcgc gatgaatgtt ctgtactcgc tgcagcacct
     2821 tgggtacgcg atcccgcccc aggccgatgc cgggtggatt gggccggcgg gtcccggtcc
     2881 ctcctacctg gatgagggat ccggcgggcc gcagaacgac ttcacgcagc gcaacaccac
     2941 cttcatgacc tggaatctca tgcacctggc ggcggcgctc aagcgcggag ggggtttccc
     3001 ggcccacggc aaccagcgca gcgagtggga tgccggtgcc cggttcgatc acccgaaccc
//
LOCUS       DASRKH010000058         5979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_63747, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000058 DASRKH010000000
VERSION     DASRKH010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 5979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5979
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63747"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VFC06_00965"
     CDS             <1..467
                     /locus_tag="VFC06_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80507.1"
                     /translation="HRMFVWVPNTRTWHRSRELETDYLIERELTFEPLAAGDVPEAMS
                     AANRIDERSAGWLIEEYRTQPPADRRTSSDLGLRLSGERATTAQVLVERLAATTDWVA
                     VKTYAHTAERSAASLASDIRRGQRKALAKLGPLDARVSPAGSDLVVEARRLA"
     gene            470..1381
                     /locus_tag="VFC06_00970"
     CDS             470..1381
                     /locus_tag="VFC06_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80508.1"
                     /translation="MQTALDSTTGRVWSAEAYARTPQALRPHHLECDGHTATGHCRTP
                     VHFRSASVNGRRPCFYGKHQPECTAASLASEDDDAGPGQPTVALASRVRWLMINLDPP
                     TPSSGPDGRRRPDTDPLGEPVRRRTPAEGLTTTESTLRPRLRAILATLLATGYPPGAL
                     VRLDGFPVLPVEAFFVRLDIAVPTAACGLRRGYYGRVRTITPDPRDGSWLIKVHDSEI
                     SIAATAAAVAGRTTAGLVGGHVLALGHPREAKRSPGRYHVKITEPALLEVQPAARAGA
                     SGLGMNRASARSQGQGQESGAGRPHSA"
     gene            complement(1448..1825)
                     /locus_tag="VFC06_00975"
     CDS             complement(1448..1825)
                     /locus_tag="VFC06_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80509.1"
                     /translation="MGAHRTGHGWLEEARDGDRTRSWHPGAVTITRRTALFAQIAQQT
                     DEQLRERGIVVEPQALDAVINHRVDQVAGRLRVSARTALLYAPADLPTTLVNLIVDAI
                     NTSSNEPAEGTRRGGLRVVDERP"
     gene            complement(1888..2637)
                     /locus_tag="VFC06_00980"
     CDS             complement(1888..2637)
                     /locus_tag="VFC06_00980"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HZL80510.1"
                     /translation="MISSTSQQYALRTTRWIVPVMLGFSLTLTAWWATSTAANSLPAV
                     GAFDVASPRAPIAAALVVVIVATVLLPVLGEPRLVAAELRAGGTRLVRVLGLAPRTGL
                     VRLGLVGVGAGVVALLVAGALIRVPVLAETNVVDARDTAISGAHPVVAGMYLALHGAV
                     WEELFYRGPVLVVAWVTARARPTVRWPLTIGAILLSSALFGAAHLSWSLANAVTAAAT
                     GVIFGAVAVARRSLWPAMLAHGTMNFLIGLM"
     gene            complement(2703..2942)
                     /locus_tag="VFC06_00985"
     CDS             complement(2703..2942)
                     /locus_tag="VFC06_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HZL80511.1"
                     /translation="MRPAALKALRKIDRQDAARIQGAIALLAQDPRPPGAKALRGRPA
                     LRVRVGDYRIIYTVQDDVLLVVVVTLGHRRDVYSR"
     gene            complement(2960..3208)
                     /locus_tag="VFC06_00990"
     CDS             complement(2960..3208)
                     /locus_tag="VFC06_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007272674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HZL80512.1"
                     /translation="MSTVSVTDARSRLPQVIDQSRTEAVFLERRGKLEAVVVSPEQYE
                     RMVEALDEADDAAAFDAAMAEEGPNVPWEQVKADLGWV"
     gene            complement(3306..4433)
                     /locus_tag="VFC06_00995"
     CDS             complement(3306..4433)
                     /locus_tag="VFC06_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017197878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HZL80513.1"
                     /translation="MSDSALERELRAAAELAEAAARVERDQDEPGDGVDGYGELLADL
                     KARVRATQFRAARAANSEVLRLYWSIGRDIRERQQSAGWGTKVIARLAADLRREFPDQ
                     RGWSPSNLKNMRRVAEVWPTLEEFGQQAVGRLPWGHVIVLLERLSTRDERDWYAAAAA
                     ENGWTRGVLELQIRSGLRSALGAAPTNFTAALDSADSELAQQMVKDPYVFEHLAFVKS
                     LAERDVEQALMDRLQETMLELGRGMAFVGRQVRLTVPDDRTDAVSEFQVDLLFFHVTQ
                     LRYVVVELKVGPFEPAHLGQLGTYVAIVDDQYRRPGIHAPTVGILLCTGKNGPVVRYS
                     LASTAAPIAVADYYGLPDDARAALPTAEQLRDALDLDGHDR"
     gene            complement(4477..5439)
                     /locus_tag="VFC06_01000"
     CDS             complement(4477..5439)
                     /locus_tag="VFC06_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80514.1"
                     /translation="MLKRLGLVLVAITLAAPLTASAATAGGTIECPPGTVQDDAGRCI
                     ISVDDPGDPGDPGNPPGENDGGDGDPLPCTFGEREIPCTLDGYYWIASRGCYARAADP
                     QPPLTESVWGGRTDGVIVTCVQPSCLGQDTGECYSEIYWAAAAPGAGPTPRQLADRAV
                     AAMDFRAPEIGLTGKSDDPDSMQIIGLPTWMWVADPDEHTVGPITRGASAGGITVTAT
                     GTLDKVMWSMGDGRTETCTGSEAQGTPYGDSYNDQPSPTCGHRYLRTSAGQPHEAFTV
                     TATSYWTITWVGGGQSGTIPLTFARTSQLRVGELQVIVTDGTGR"
     gene            complement(5433..>5979)
                     /locus_tag="VFC06_01005"
     CDS             complement(5433..>5979)
                     /locus_tag="VFC06_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80515.1"
                     /translation="SVCAALVTTTAACTHNDPTPSPATTTATSTPTPTPTPTPTLTLS
                     AEQQQIEDAKAAYKTYRSTTNEVEQAGMADWRERILPLVGGDMREKKVSFYEQAEQQG
                     LRQTGEIAIAGMTVIASTDRTVTLEVCHDNTGADLVNPAGESVLLAGYASRLISTIEM
                     FQQDDGRWTVNSGEADEERPC"
BASE COUNT          900 a         2118 c         2061 g          900 t
ORIGIN      
        1 atcaccgcat gttcgtctgg gtccccaaca cccgcacatg gcaccgctcg cgcgagctgg
       61 agaccgacta cctgatcgag cgcgagttga ctttcgagcc gctggcggcc ggagatgtcc
      121 ccgaggcgat gagcgccgcc aaccgcatcg acgagcgctc tgcaggatgg ctgatcgagg
      181 agtaccgcac gcagccaccc gccgaccgtc gcaccagctc ggacctcgga ctgcgcctct
      241 ccggcgagcg ggccaccacc gcccaggtgc tggtcgagcg cctcgccgca acgacggact
      301 gggtcgcggt caagacctac gcccacaccg cggagcggtc ggcggcttcg ctggccagcg
      361 acatccggcg tgggcagcgc aaggcactgg ccaagctcgg ccccctggac gcccgcgtgt
      421 cgcctgccgg cagcgacctg gtcgtcgagg cgcgacgcct ggcctgagcg tgcagacagc
      481 tctggactcg acgacgggcc gcgtctggtc ggccgaggcc tacgcccgga ctccgcaagc
      541 gctacggccg catcacctcg agtgcgacgg acacacggca accgggcact gcaggacgcc
      601 ggtccacttc cgcagcgcgt ccgtcaacgg ccgccgaccg tgcttctacg gcaagcacca
      661 gccggagtgc accgcagcct cgttggccag cgaagacgac gacgcgggcc ccggccagcc
      721 cacggtggcg ctggccagcc gcgtgcgctg gctgatgatc aacctggatc cgcccacacc
      781 ctcgtcgggg cccgacgggc gccgacgccc cgacaccgac ccgctcggcg agccggtccg
      841 tcgtcgcacc ccggccgagg gcctgacgac caccgagagc accctgcgcc cacgcctccg
      901 agcgatcctg gccacgctgc tggcgaccgg atacccaccg ggcgcgctcg tgcgcctcga
      961 cgggttcccg gtcctgcctg tcgaggcgtt cttcgtccgc ctggacatcg ccgtgcccac
     1021 cgcagcgtgc ggcctgcgcc gtgggtacta cgggcgcgtc cgcacgatca cccctgatcc
     1081 ccgggacggc agctggctga tcaaggtcca cgacagcgag atctcgatcg ccgcgaccgc
     1141 agctgcagtc gcgggccgga ccacggccgg gctcgtcggc gggcatgtcc tggcgctcgg
     1201 gcaccctcga gaggccaagc ggtcccccgg caggtaccac gtcaagatca ccgagccggc
     1261 gctgctcgag gtgcagccgg cggcccgggc cggagcctct gggctgggca tgaacagggc
     1321 cagcgcccgc tcgcaggggc aggggcagga gtcgggcgct ggccgacccc atagtgcctg
     1381 accaaggcgc cgaccggagg ccgttcaccc gaacggcgtc gtgcagcgcc atgctgcacg
     1441 agcgaggtca gggtcgctcg tcgacgacgc gcaggccgcc acgtcgagtg ccttcagcgg
     1501 gctcgttgct gctcgtgttg atggcgtcga cgatcaggtt gacgagcgtg gtcggcaggt
     1561 ccgccggcgc gtacagcagc gcggtgcgcg cggatacccg cagtcggcct gcgacctggt
     1621 cgacccggtg gttgatcacg gcgtcgagag cctgtggttc gaccacgatc ccgcgctcgc
     1681 gcagttgctc gtcggtctgc tgcgcgatct gcgcgaacag cgcggtgcgc cgggtgatgg
     1741 tcacggcgcc aggatgccac gaccgcgtcc ggtcgccgtc gcgggcttcc tcgagccagc
     1801 catgaccggt gcggtgggca cccacaggtg gtcccctacc gatcacgcct ggcagccgtg
     1861 ctgagcggtg gcgacgtcgc tggtcggcta catcaagccg atgaggaagt tcatggtccc
     1921 gtgggcgagc atcgccggcc acagggatcg cctcgctact gcgacggccc cgaagatcac
     1981 acccgtcgcg gcggccgtca ccgcgttcgc cagggaccac gacagatgag cagcgccgaa
     2041 gagggcgctg ctgagcagga tcgcgccgat ggtcagtggc caccggaccg tcggcctggc
     2101 ccgagcagtc acccaagcca cgaccaacac cggcccgcgg tagaagagtt cctcccagac
     2161 cgcgccgtgc aaggccaggt acatgcccgc caccacgggg tgcgcaccag agatcgcggt
     2221 gtcccgcgcg tcgaccacgt tcgtctcggc gaggaccgga acacggatga gcgcccctgc
     2281 gaccagaaga gcgacgacac ccgcgcctac gccgacgagg cccagacgca ctaacccggt
     2341 gcgcggggcc agccccagca cccggaccag gcgtgtgccg ccggctcgca gctccgccgc
     2401 gacgagccgt ggttcgccga gcaccgggag caggacggtc gccacgatca cgacgacgag
     2461 cgctgccgcg atcggtgccc gcggcgaggc gacgtcgaac gcaccgaccg ctggcagcga
     2521 gttcgccgcg gtgctcgtcg cccaccacgc ggtcagcgtc agggagaaac ccagcatgac
     2581 cgggactatc caccgcgtgg tccggagcgc gtactgctgc gacgtcgagg agatcaccgc
     2641 cgcagtgtgg cagcggccgc ccacaccagc ccagcttcgt ggtggccaac cgggcatcgc
     2701 gctcagcgcg agtagacgtc gcgtcggtgc ccgagcgtga ccacgacgac caggagcaca
     2761 tcgtcctgga cggtgtagat gatccggtag tcccctactc gtacccgaag cgctggccgg
     2821 ccgcggagcg ccttcgcacc cggtgggcgt gggtcttggg caaggagcgc gatcgcgccc
     2881 tggatccgcg ccgcgtcctg ccggtcgatc ttccgcagcg ccttgagggc cgccgggcgc
     2941 atctcgatcc ggtacgcgct cacacccagc ccaggtcggc cttgacctgc tcccagggca
     3001 cgttggggcc ctcttcggcc atcgcggcat cgaacgccgc cgcgtcgtcg gcctcatcaa
     3061 gggcctcgac catgcgttcg tactgctctg ggctcacgac gacggcctcg agcttgccgc
     3121 gtcgctcgag gaacaccgcc tcggtgcgcg actggtcgat gacctgcgga agccggctac
     3181 gcgcgtcggt gacgctcacg gtgctcatac cacgaatgta cgttcagacg gccaagatgt
     3241 acatcggacg ggcgcgcgac ctcgttcgtg caccaccgcg gtgcacgaac acggggcgaa
     3301 gccactcacc ggtcgtggcc atcgaggtcg agcgcgtcgc gcagttgctc agctgtgggc
     3361 agcgccgcgc gcgcgtcgtc gggcagcccg tagtagtccg cgactgcgat cggcgccgcg
     3421 gtcgacgcga gcgagtaccg cacgaccggg ccgttcttgc cggtgcacag caggatcccg
     3481 acggtgggcg cgtggatccc gggtcggcgg tactggtcgt cgacgatcgc gacgtaggtg
     3541 ccgagctggc ccaggtgcgc cggctcgaac gggccgacct tgagctcgac caccacgtac
     3601 ctgagctggg ttacgtggaa aaagagaagg tcgacttgga actccgagac agcgtcggtg
     3661 cggtcgtcag gcacggtgag gcggacctgg cgcccgacga acgccatgcc gcgcccgagc
     3721 tcgagcatcg tctcctgcag gcggtccatc agcgcctgct cgacgtcccg ctcagcgagg
     3781 ctcttgacga aggcgaggtg ctcgaagacg tacggatcct tgaccatctg ttgcgcgagc
     3841 tcggagtccg ccgagtcgag ggccgcggtg aagttcgtcg gggccgcacc cagcgcgctg
     3901 cgaaggcccg agcgaatctg cagctcgagc acaccgcgtg tccagccgtt ctcggccgcg
     3961 gccgcggcgt accagtcccg ctcatcgcga gtgctgagcc gctccaagag gacgatcacg
     4021 tgaccccagg gcaatcggcc aacagcttgt tggccaaact cctcgagcgt gggccagacc
     4081 tccgcgaccc ggcgcatgtt cttcagattg gacggtgacc atccccgctg atcggggaat
     4141 tcgcggcgta ggtcagccgc aaggcgagca atcaccttgg tcccccaccc agccgactgc
     4201 tgccgctccc gaatgtcgcg accgatcgac caatacaggc gcagcacctc ggagttcgcc
     4261 gcccgagccg cccggaactg ggtcgcgcgc acccgcgcct tgaggtccgc gagcagctcg
     4321 ccgtacccgt cgacgccgtc gcccggctcg tcctggtcgc gctcgactcg cgcggccgcc
     4381 tccgcgagct cggccgcggc acgtagctcg cgctcgagcg ccgagtcact catcggcatc
     4441 ccgctcgggg acccgactgc ttcggtcgag tcggtctcac cggccggtcc cgtcggtgac
     4501 gatgacctgg agctcgccca cccgcagctg cgaggtgcgc gcgaaggtca gcgggatggt
     4561 gccggactgg ccgccgccga cccaggtgat cgtccagtac gacgtcgccg tcaccgtgaa
     4621 cgcctcatgc ggctgaccgg ccgacgtgcg gaggtagcgg tgaccgcagg tcggtgacgg
     4681 ctggtcgttg tagctgtcac cgtacggggt gccctgtgct tcgctgccgg tgcaggtctc
     4741 ggtgcggccg tcgcccatgc tccacatgac cttgtcgagt gtcccggtgg ctgtgacggt
     4801 gatcccgccg gcgctggcgc ctcgggtgat tggaccgacg gtgtgctcgt cggggtcggc
     4861 tacccacatc caggtgggca gaccgatgat ctgcatcgag tcggggtcgt ccgacttgcc
     4921 ggtcaggccg atctccggcg ctcggaagtc catcgcggcg actgctcggt cggcgagctg
     4981 gcggggggtc ggtccggcac ctggcgccgc tgctgcccag tagatctccg agtagcactc
     5041 cccagtgtcc tgtcccaggc aggagggctg cacgcacgtg acgatcacgc cgtcggttcg
     5101 cccaccccac acactttcag tgaggggagg ctggggatcg gccgcccgtg cgtagcaccc
     5161 gcggctagcg atccagtagt agccgtcgag cgtgcagggg atctcccgtt caccgaaggt
     5221 gcacgggagc gggtcaccgt cgccaccgtc gttttcaccg ggtggattgc cagggtcacc
     5281 cgggtccccg ggatcatcga ccgagatgat gcaccgcccg gcatcatctt ggacggtgcc
     5341 gggcgggcac tcgatggtgc cgcccgcggt cgctgcagat gctgtcagcg gtgcggcaag
     5401 agtgatcgca accaagacga ggccgagccg cttcagcatg gccgctcctc atcggcttcg
     5461 ccggagttga cggtccatcg accatcgtct tgctggaaca tctcgatggt cgagatcagc
     5521 cgagacgcgt acccggctag caataccgac tcgcccgcgg ggttaaccag atcggcgccg
     5581 gtgttgtcgt gacacacctc aagcgtgaca gtcctgtcgg tcgacgcgat gactgtcatg
     5641 cctgcaatgg ctatctcgcc tgtctgccgc agtccctgct gctcggcctg ctcgtagaag
     5701 gacaccttct tctcgcgcat gtctccgccg accagcggga gaatccgctc gcgccagtcg
     5761 gccatgccgg cctgctcgac ttcgttggta gtggaccggt aggtcttgta ggcagccttg
     5821 gcgtcctcga tctgctgctg ctcagcgctg agcgtgaggg tgggcgttgg cgttggcgtg
     5881 ggtgtcggtg tgctggttgc cgtcgtagta gctggcgagg gtgtcgggtc gttgtgggtg
     5941 cacgcggctg tggtggtgac gagcgcggcg caaacgctg
//
LOCUS       DASRKH010000059         3947 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_49425, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000059 DASRKH010000000
VERSION     DASRKH010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49425"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..129)
                     /locus_tag="VFC06_01010"
     CDS             complement(<1..129)
                     /locus_tag="VFC06_01010"
                     /inference="COORDINATES: protein motif:HMM:NF023324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltotransferase domain-containing protein"
                     /protein_id="HZL80516.1"
                     /translation="MERSPELSVGRIPIVDVQPSLEQGRWPARAVVGEAVPVTATIF"
     gene            complement(220..1197)
                     /gene="galE"
                     /locus_tag="VFC06_01015"
     CDS             complement(220..1197)
                     /gene="galE"
                     /locus_tag="VFC06_01015"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HZL80517.1"
                     /translation="MTAWLVTGGAGYIGAHVVSALLDAGFEAVAYDNLSTGKREFVSD
                     GAPLVEGDILDTDAVTSALTAHGCVGVIHCAGYKYAGESVKYPTLTYLQNVSGTATLL
                     EAMSAAGVRSLVFSSSAGVYGTPDTEVVTEATECRPESPYGESKLVAEWLIRDQVTAT
                     ASSDTPLAATSLRYFNVVGSGPSNIYDVSPHNLFPKVFATLTRGEPARINGDDYSTPD
                     GTCVRDYVHVVDLANAHVAAARALDEGRPLEPVYNLGSGTGTSVREIVDAMCEVTGNT
                     TAPLIGPRRPGDPARIVAAGDLATRDLGWEPRSTIEHMVRTAWDAMPHA"
     gene            complement(1228..2385)
                     /gene="galK"
                     /locus_tag="VFC06_01020"
     CDS             complement(1228..2385)
                     /gene="galK"
                     /locus_tag="VFC06_01020"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HZL80518.1"
                     /translation="MTIPAGLAERFASLYGAPPVGIWSAPGRANLIGEHTDYNNGYVL
                     PFAIDDRTWCAARPNTVGLLRVASTAQTSRHSVSIADLDQASFEGWSGYALGVAWALH
                     KAGHDLSDSPGIDLLVDSTVPLGAGLSSSAALESAVAVALDDLWDLGLDQMALAKAGQ
                     LAENGAIGAPTGIMDQVATLFGQEDHAVLLDCASLRTTAVPLRLAASGLSLLVLDSKV
                     KHAHADGGYASRRQACELGARTMGVPFLRDLTVADLEKAQSVLDDETFRRVRHVVTEN
                     DRVLATVEALATSGPESIGRLLLASHASMRDDFEISVPEIDTAVEASMSADAVGARLT
                     GGGFGGSAIALVPTDRVSGLGDAVRAACSERGFAIPEIRIVNPCQGARRDA"
     gene            complement(2580..>3947)
                     /locus_tag="VFC06_01025"
     CDS             complement(2580..>3947)
                     /locus_tag="VFC06_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme GlgX"
                     /protein_id="HZL80519.1"
                     /translation="DLLHAAGIEVILDVVYNHTMEGGWGDRVVSWRGLDNFAYYRHSP
                     MNPMHYDDVTGTGNSLDFSQPVVVKMALDSLRYWTEVMGVDGFRFDLAVTLGRNMNGF
                     STDHPFLVALTTDPILGSTKLIAEPWDIGLGGWQVGNFPISMTEWNDRFRDYVRSYWL
                     TFGAGKVGGRDHATAPELATRLAGSADLFGHTEPYGMRGPMASINFVTAHDGFTAYDL
                     TAYNDKHNEANGEHNRDGTDNNRSYNHGAEGPTDDVEVLAARRRSLRNLLGTLLLSAG
                     TPMLLGGDEMGRTQRGSNNAYCQDNEISWFDWEREPWQLELQDTVAHLIALRTRHLVL
                     RAERFYEGVDIDPRDQDLRADSAWFTISGEHEDDDWWEAPGTRVLQFMRSPSQPDEAD
                     ALILINGSREDASVTIPDDDGPLWNLAWDSAWESPAEHTDDLTAPGSVTQMPALTMRL
                     YVSAI"
BASE COUNT          645 a         1226 c         1389 g          687 t
ORIGIN      
        1 gaagatggtg gccgtgacgg gtacggcttc tccgacgacg gcgcgggcgg gccagcgtcc
       61 ctgttcgagg gacggttgca cgtccacgat ggggatccgg ccgacagaca actccgggct
      121 tcgctccatg ccatgaacct actcgcctcg gcgttcgcta ccaagacggc tggcgctggc
      181 tgtggtgcga atcgtgttca caggcaggcc gaggggtcgc taggcgtggg gcatggcatc
      241 ccacgcggtc ctcaccatgt gctcgatcgt ggagcggggt tcccatccaa gatcgcgtgt
      301 ggcgaggtcg ccagccgcga cgatgcgcgc cgggtcgccc ggacggcgcg ggccaatcag
      361 gggtgcagtg gtgttgccgg tcacctcgca catcgcgtcg acgatctcgc ggaccgaggt
      421 gcccgttccc gaaccgaggt tgtagaccgg ctccaacggt cggccctcat ccagggcgcg
      481 agccgccgcg acgtgggcgt tggcgaggtc cacgacatgg acgtagtcgc gcacgcacgt
      541 gccgtcaggg gtgctgtaat cgtcgccgtt gatccgcgcg ggctcgccgc gcgtgagcgt
      601 ggcaaagacc ttggggaaga ggttgtgtgg cgagacatcg tagatgttcg aaggcccgga
      661 tcccaccacg ttgaagtagc gcaggctcgt ggcggcgagc ggcgtatcgg aggatgccgt
      721 ggcagtcact tggtcgcgga tgagccattc cgccaccagc ttgctctcgc cgtaggggct
      781 ctccgggcgg cattcagttg cctccgtgac gacttcggtg tccggagttc cgtacacccc
      841 cgccgacgag gagaacacga ggctgcggac gcccgccgcg gacatcgcct cgagcagggt
      901 ggcggtaccg gagacgttct ggaggtaggt gagggtgggg tatttcaccg attcgccagc
      961 gtatttgtag cctgcgcagt gaatgacgcc gacgcagccg tgcgccgtca gcgccgacgt
     1021 gaccgcgtcg gtgtcaagga tgtcgccctc gacgagcggc gctccgtcgg acacgaactc
     1081 gcgcttgccg gttgacaggt tgtcgtacgc gacggcctcg aaccccgcgt cgagaagcgc
     1141 ggagaccacg tgggcgccga tgtagccggc gcccccggtg acgagccagg cggtcatgac
     1201 gcagttcctt gggccatgcg gctaaggcta cgcgtcacgg cgggctcctt gacaggggtt
     1261 cacgatgcga atctccggaa tggcgaatcc gcgctcactg cacgcggcgc ggacggcgtc
     1321 gccgaggcct gagacgcggt cggtgggcac aagcgcgatc gccgagccgc cgaagccgcc
     1381 tcccgtgagg cgtgcgccca cggcgtctgc actcattgat gcctcgaccg cagtgtcgat
     1441 ttcgggaacg gagatctcga aatcgtcccg catggacgcg tgggaggcga gcaggagacg
     1501 cccgatggac tcggggcccg aggtggcgag agcctccaca gtggctagga cgcggtcgtt
     1561 ttcggtcacg acgtggcgta cgcggcgaaa ggtctcgtcg tcaaggacgg actgtgcttt
     1621 ttcgagatct gccaccgtca gatcgcgcag gaatggcacg cccatcgtgc gcgcgccgag
     1681 ctcgcacgcc tgtcgcctcg acgcgtagcc gccgtcggcg tgggcgtgct tcaccttgga
     1741 gtcgagcacg aggagcgaga gtccgctggc ggcgaggcga agcgggacag cggtggtgcg
     1801 caacgatgcg cagtcaagca gcacggcgtg gtcttcctgg ccaaagagcg tggcgacctg
     1861 gtccatgatg cccgtgggtg cgccgatcgc gccgttctcg gccagctgcc cagccttggc
     1921 gagcgccatc tgatccagcc cgaggtccca taggtcgtcg agggcgacgg cgacggctga
     1981 ttcgagggcc gcggacgacg acagtcctgc gccgagcggg acggtggaat cgaccaggag
     2041 gtcgatgccg ggggagtctg agaggtcgtg gcccgctttg tgtaaagccc acgccacccc
     2101 tagtgcgtag cccgaccagc cctcaaagct cgcttggtcg aggtcagcga tcgagacgct
     2161 gtggcgcgag gtctgagccg tgctggcgac gcgcaagagg cccacggtgt tggggcgcgc
     2221 cgcacaccag gtgcggtcgt cgatggcgaa gggaagaacg tagccgttgt tgtagtccgt
     2281 gtgctcgccg atgaggttgg cgcggccagg cgcggaccag atgccgacag gtggtgcgcc
     2341 atagagggac gcgaatcgct cggcaagtcc agcggggatc gtcatgattg gccgcgattc
     2401 ttctcgacga ccaacgccgc aagagtggtt gtggtggtgt caggctcgcg catcgcgctt
     2461 cgaccccctt cggtcggcat gaatagcacc aatgttaacg ttctcatttc acagtacacc
     2521 agcgatccga cagaagagtc gcgcgccgac tccggtcggt cacccggctc gcggcgttgc
     2581 tagatggcgc tgacgtaaag gcgcatcgtc agggcgggca tttgcgtcac agagcctgga
     2641 gccgtgaggt cgtccgtgtg ctcggctggg ctctcccaag cggagtccca cgccaggttc
     2701 cacaaggggc cgtcgtcatc gggaatcgtg accgaggcgt cctcgcggga gccgttgatc
     2761 aggatgagcg catccgcctc gtcgggctgt gacggcgacc gcatgaactg caacactctc
     2821 gtcccagggg cttcccacca gtcgtcgtct tcgtgctcgc cagagatggt gaaccacgcg
     2881 gagtccgccc ggaggtcttg gtcgcgggga tcaatatcca cgccctcgta gaaccgttcg
     2941 gcgcggagca ccaggtgccg cgtgcgcaag gcgatgaggt gggcgaccgt gtcctgcagc
     3001 tcgagttgcc acggctcccg ttcccagtcg aaccacgaaa tctcgttgtc ttggcagtac
     3061 gcgttgttgc tcccgcgctg ggtgcgaccc atttcgtcac cccccagcag catgggggtg
     3121 cctgccgaca gcaacagcgt gcctaagagg ttgcgcaggg agcgacgcct ggcggccagc
     3181 acctccacgt cgtcagtggg gccctcggcg ccgtggttgt aggaccgatt gttgtccgtg
     3241 ccgtcgcggt tgtgttcccc gttggcctcg ttgtgcttgt cgttgtacgc cgtgaggtcg
     3301 tacgcggtga agccgtcgtg tgcggtcacg aagttgatcg acgccattgg tccgcgcatc
     3361 ccgtagggct cggtgtggcc aaacaggtcg gccgatccgg cgagtcgcgt ggcgagttcc
     3421 ggcgccgtgg cgtgatcccg tccgcccacc ttgcccgcac cgaaggtgag ccagtacgag
     3481 cgcacgtagt ctcgaaagcg gtcgttccat tccgtcatcg aaatggggaa gttgccaact
     3541 tgccagccgc ccaggccgat atcccacggt tcggcaatga gcttggtcga gccgaggatc
     3601 gggtccgtcg tcagcgccac caggaacggg tggtcggtgg agaagccgtt catgttgcgg
     3661 ccaagggtga ccgctaggtc aaagcggaag ccgtcgacgc ccatgacctc ggtccagtac
     3721 cgcaaagagt ccagcgccat cttgacgacg acgggctgcg aaaagtcgag cgagttgccc
     3781 gtgcccgtca catcgtcgta gtgcatcggg ttcatcgggc tgtgacggta gtaggcaaag
     3841 ttgtccagtc cgcgccaact gaccacccgg tcgccccagc caccctccat cgtgtggttg
     3901 tagaccacgt caagaatgac ctcgatgccg gcggcgtgga gcaggtc
//
LOCUS       DASRKH010000060         2093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_23822, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000060 DASRKH010000000
VERSION     DASRKH010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23822"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..1040
                     /gene="pdxS"
                     /locus_tag="VFC06_01030"
     CDS             123..1040
                     /gene="pdxS"
                     /locus_tag="VFC06_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HZL80520.1"
                     /translation="MSEAFVTIENNQPVVGTAKVKRGMAEMLKGGVIMDVVTADHAKI
                     AEDAGAVAVMALERVPADIRAQGGVARMSDPDLIDGIIAAVSIPVMAKARIGHFVEAQ
                     VLQSLGVDYIDESEVLTPADYAHHIDKWAFTVPFVCGATNLGEALRRIGEGAAMIRSK
                     GEAGTGDVSNATKHMRTLRDEIRRLTSLPEEELYLAAKDLQAPYDLVVEVAKTGKLPV
                     VMFTAGGIATPSDAAMMMQLGAEGVFVGSGIFKSGNPAERAVAIVKATTFFDDPSVIA
                     SVSRGLGEAMVGINVADLPAPHRLADRGW"
     gene            1037..1633
                     /locus_tag="VFC06_01035"
     CDS             1037..1633
                     /locus_tag="VFC06_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HZL80521.1"
                     /translation="MTSGQARKPADEGSPGVEGSGSHLGSEWSLGVDGLRYRRAARVI
                     LLDRSDRVLLLRGHDVDQRERHWWFTIGGGIGVGEDARAAVVRELREETGVRLDPGEL
                     VGPVFTRSAIFDFYAEHCRQDEEIFLARTEAMDVAVLSRDGWTDIELDVLDGLRWWDL
                     DALDAVTDEVYPLGLARLVRGLLNGWDGKVRHLEEGRR"
     gene            1684..>2093
                     /gene="pdxT"
                     /locus_tag="VFC06_01040"
     CDS             1684..>2093
                     /gene="pdxT"
                     /locus_tag="VFC06_01040"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HZL80522.1"
                     /translation="MTPTVGVLALQGDVREHVRMLERCGVDAVPVRRERELGAVDGIV
                     IPGGESSTIDKLLRAFELFEPLQKAIRDGLPAFGSCAGMILLADRVLDGIEGQQTLGG
                     LDVTVRRNAFGRQVDSFEAELAVEGVEGARMNTA"
BASE COUNT          310 a          698 c          765 g          320 t
ORIGIN      
        1 ttcgagatcg acgacgcctg gcccgacgcc ctcggcaagt cggcgccggc ctcacgccag
       61 tagcgagtgc ggactacgat gacggcatgc cgctgcgtcg cggcccaatc cctgtcatcc
      121 ggatgagcga ggcctttgtg accatcgaga acaaccagcc cgtcgtcggc actgccaagg
      181 tcaagcgcgg catggccgag atgctcaagg gcggcgtgat catggatgtg gtcaccgccg
      241 accacgcgaa gatcgccgag gacgccgggg cggtagccgt gatggctctc gagcgggtgc
      301 ccgcagacat ccgcgcccag ggcggcgttg cccggatgag cgacccggat ctgatcgacg
      361 gcatcatcgc tgccgtctcg atcccggtga tggccaaggc acgcatcggg cacttcgtcg
      421 aggcgcaggt cctccagtcg ctcggcgtcg actacatcga cgagtccgag gtgctcaccc
      481 cggccgacta cgcgcaccac atcgacaagt gggccttcac ggtgccgttc gtgtgcggtg
      541 cgaccaacct cggcgaggcg ttgcggcgga tcggcgaggg cgccgcgatg atccgctcca
      601 agggcgaggc cggcaccggc gacgtctcga acgcgaccaa gcacatgcgc accctgcggg
      661 acgagatccg gcgcttgacg tcgctacccg aggaggagct gtacctcgcc gcgaaggacc
      721 tgcaggcgcc gtacgacctc gtggtggaag tcgcgaagac aggcaaactg ccggtggtga
      781 tgttcaccgc cggcggcatc gccaccccgt ccgacgccgc gatgatgatg cagctgggag
      841 cggaaggcgt cttcgtcggc tcgggcatct tcaagtccgg gaaccccgcc gagcgcgcgg
      901 tcgcgatcgt caaggccacc acgttcttcg atgacccgag cgtgatcgct tcggtgtcgc
      961 gcggcctggg tgaggccatg gtgggcatca atgtggccga cctcccggcg ccgcaccggc
     1021 tggccgatcg cggctggtga catcgggtca ggcccgcaag ccggcggacg agggttcgcc
     1081 gggagtcgag ggttccgggt cgcacctggg atccgagtgg tccctcggcg tggacggcct
     1141 gcgctatcgc cgcgctgcgc gggtgatcct gctcgaccgc tccgatcgtg tcctgctgct
     1201 ccgcgggcat gacgtcgacc agagggaacg tcactggtgg ttcacgatcg gcggtgggat
     1261 cggcgtcggc gaggacgccc gtgccgcggt ggtgcgcgag ctgcgtgagg agaccggagt
     1321 ccggctggac cccggcgagc tggtcggccc cgtcttcacc cgctccgcga tcttcgactt
     1381 ctacgccgag cactgccggc aggatgagga gatcttcctg gcccggaccg aggcgatgga
     1441 cgtcgccgtc ctgagccggg acggttggac cgacatcgag ctggatgtgc tcgacgggct
     1501 ccgttggtgg gacctcgacg cgctcgacgc ggtgaccgac gaggtgtacc cgctcggcct
     1561 ggcccggctt gtccgcggcc tcctgaacgg ctgggacggc aaggtgcggc acctcgagga
     1621 aggccggcgc tgaggcccgc cgaccggcat cggcccccgg cccgccccgt aagattgcgg
     1681 ctcatgaccc cgactgtcgg tgtgctcgcg ctccagggag acgtgcgcga gcacgtcagg
     1741 atgctcgagc ggtgcggcgt ggacgcggtc ccggtccgcc gcgagcgtga gctcggagca
     1801 gtcgacggca tcgtgatccc ggggggcgag tcgtccacga tcgacaagct gttgcgcgcg
     1861 ttcgagctct tcgagccgct ccagaaggcg atccgcgacg ggctccccgc tttcggctcg
     1921 tgcgccggga tgatcctgct cgcggaccgg gtgctggacg gtatcgaggg ccagcagacg
     1981 ctcggcggcc tcgacgtcac ggtgcggcgc aacgcgttcg ggcgacaggt cgactcgttc
     2041 gaggcggagc tcgcggtcga gggcgtcgag ggcgcccgga tgaacaccgc gtg
//
LOCUS       DASRKH010000061         2292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_10506, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000061 DASRKH010000000
VERSION     DASRKH010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10506"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..953
                     /locus_tag="VFC06_01045"
     CDS             24..953
                     /locus_tag="VFC06_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol mannoside acyltransferase"
                     /protein_id="HZL80523.1"
                     /translation="MRAAGSIPGWIVRALCAVAADAAWLARGGGVRQLEANLRRVRPD
                     VGSRELRRLSRAGMRSYLRYYGEVFTLASLTRDQIEARVRTTGLERYAPLLDAGETIV
                     LGLGHQGNWDLAGAWATPHVARVVTVAEHLEPEELFQEFLTLREHLGLTIIPLTKNGG
                     DVFRDLVRAVRGGSGLLPLLADRDLTARGVEVSLFGETARVAAGPGALAVATGAHLAA
                     TVIWYERLHGVRRRAAGSPWGIVVEFGELIEAPTTGTRADRVQVLTQAWVDGLARGIA
                     AHPQDWHMLQKVWVDDLDADRYAATLAAQEREA"
     gene            950..2182
                     /locus_tag="VFC06_01050"
     CDS             950..2182
                     /locus_tag="VFC06_01050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZL80524.1"
                     /translation="MSAPEVAGRDRPLRVGIVCPYSFDVPGGVQFHVRDLAEALISRG
                     HTVSVLAPADDDTPIPDYMTSAGRAVPVRYNGAVARLTFGPVTAARVRRWLAAGEFDV
                     LHLHEPVTPSLGMLALWIADGPIVATFHTSLVRSRPLQMAYPLVRQSMEKISARIAVS
                     EDARRTLVEHLGGDAVVIPNGVNVEAFASAQPDERWRGTADAPTVAFLGRLDEPRKGL
                     EVLLRAVPGVLAVHPGTRFLVAGRGETGPDEARELIGDAARAVEFLGGITDEEKASLL
                     ASVDVYCAPQTGGESFGIVLVEAMSAGTAVVASDLGAFRRVLEEGTAGALFRTGDPAD
                     LARTLVRVLDDAPLREQLVTTASSAVRRYDWSSVSHEILTVYDMVVDGRVEPVGEDPS
                     SRRGGWHLDRRRSRGDER"
BASE COUNT          284 a          804 c          855 g          349 t
ORIGIN      
        1 cgggccggct cgccgttctg gggctgcgcg ccgcgggcag catccccggc tggatcgttc
       61 gtgcgctgtg cgcagtggct gcggacgcgg cctggctcgc gcgcggtggt ggagtgcgcc
      121 agctcgaggc caacctgcgc cgcgtccgcc ctgacgtcgg ctcacgtgag ctgaggcgtc
      181 tgagcagggc gggcatgcgg tcgtacctgc gctactacgg cgaggtcttc acgctcgcgt
      241 ccctgacgcg cgaccagatc gaggcgcgcg ttcggacgac cgggctcgag cgctacgccc
      301 cgctgctcga cgcaggcgag acgatcgtgc tcggtctcgg ccaccagggg aactgggatc
      361 tggccggggc ctgggcgacg ccgcacgtcg cgagggtcgt cacggtcgcc gagcacctcg
      421 agcccgagga gctcttccag gagttcctca cgttgcgcga gcacctgggg ctcacgatca
      481 tcccgttgac gaagaacggc ggggacgtgt tccgcgacct cgtccgcgcg gtgcgcggcg
      541 gttccgggct gctcccgctg ctcgcagacc gggacctgac cgcccggggc gtcgaggtct
      601 ccttgttcgg cgagacggcg cgggtggcag ccggacccgg agccctcgca gtcgcgacgg
      661 gggcgcacct cgccgcaacg gtgatctggt acgagcgctt gcacggcgtg cggcggcgtg
      721 ccgccgggtc gccctggggc atcgtcgtcg agttcggcga gctcatcgag gccccgacca
      781 cgggcacgcg cgccgaccgg gtccaggtcc tcacgcaggc ctgggttgac ggcctcgcac
      841 gcggcatcgc ggctcatccg caagactggc acatgctgca gaaggtgtgg gtcgacgacc
      901 tcgacgccga ccgctatgcc gcgaccctcg cggcacagga gcgcgaggcg tgagcgcgcc
      961 ggaggtcgcc gggcgcgacc gaccgttgcg ggtgggcatc gtgtgcccgt actcgttcga
     1021 tgttcccggg ggagtccagt tccacgtccg ggacctggcg gaggccctca tctcccgcgg
     1081 gcacaccgtg tcggtcctcg cccccgccga cgacgacacc ccgatccccg actacatgac
     1141 gtccgccggg cgagccgtgc cggtgcggta caacggcgcc gtcgcccgac tgacgttcgg
     1201 gcctgtgacc gccgcgcgcg tccgtcgctg gctcgcggca ggggagttcg acgtgctcca
     1261 cctgcacgag ccggtgacgc cgtcgctggg catgctcgcg ctctggatcg cggacggacc
     1321 gatcgtcgcg acgttccaca cgtccctcgt gcggtcgagg ccgctgcaga tggcgtaccc
     1381 gctggtccgc cagtcgatgg agaagatctc ggcgcggatc gccgtgtccg aggacgcgcg
     1441 ccgcacgctg gtcgagcacc tcggcggcga cgccgtcgtg atccccaacg gggtcaacgt
     1501 cgaagcgttc gcgagcgccc agcccgacga gcggtggcgg ggcacagccg atgcccctac
     1561 ggtcgcgttc ctcggtcgcc tggacgagcc gcgcaaaggt cttgaggtcc tgctccgggc
     1621 cgtgcccggg gtgctggcag tgcatcccgg cacgcgcttc ctcgtcgcag gacgcggcga
     1681 gaccgggccc gacgaggctc gtgagctgat tggcgacgcc gctcgcgccg tcgagttcct
     1741 cggcggcatc actgacgagg agaaggcgag cctgctcgcg tcggtcgacg tgtactgcgc
     1801 cccccagacc gggggtgaga gcttcgggat cgtgctcgtg gaggcgatga gcgccggcac
     1861 cgccgtcgtc gcgagcgacc tcggtgcgtt ccgccgggtc ctcgaggagg gcaccgccgg
     1921 ggcgctgttc cgcaccggcg acccggccga cctggcacgc acgctcgtcc gggtgctcga
     1981 cgatgccccg ctgcgcgagc agctggtcac gaccgcgagc tctgcggtcc ggaggtacga
     2041 ctggtcctcg gtgagccacg agatcctgac cgtctacgac atggtcgtgg acggtcgcgt
     2101 cgaaccggtc ggagaggacc cgtcgtcgcg tcgcggcgga tggcacctcg accgtcgtcg
     2161 gagtcggggt gacgagcgat gagctggtcg gagatcgtcg tcgtcgtcgt cgcagtggtc
     2221 ctgctggtcg gatggcgcct gtgggtgtcg gcctcgcggc tcgatcggct ccaccgcaag
     2281 gtggcgtcgt cc
//
LOCUS       DASRKH010000062         2924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_53739, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000062 DASRKH010000000
VERSION     DASRKH010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53739"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(305..>2924)
                     /locus_tag="VFC06_01055"
     CDS             complement(305..>2924)
                     /locus_tag="VFC06_01055"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019812 - type I site-specific
                     deoxyribonuclease complex [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HZL80525.1"
                     /translation="SMPFGESGEHVTVRLIDFADLERNEYVVTTQFTIRAGATEKRTD
                     LVLLVNGIPLVVMEAKTPVRASQSWLDGALQVHDDYERNVPELFVPNLLSVATEGKEF
                     HYGSIGLPVEMWGPWWVDADEKTPALHRIEEAVASMLRPNIILDLLANFTAYATDTKK
                     RRIKIIARYQQYEGTNKIVERVVAGHPKKGLIWHFQGSGKSLLMLFAARKLRLHPALK
                     NPTVMIVVDRIDLDTQISSTFYAADTPNLVKADSRAELAQLLAQDVRKIIITTIFKFG
                     EAEGVLNDRANIIAMVDEAHRTQEGDLGRKMRDALPNAFLFGLTGTPINRADRNTFYA
                     FGADEDTSGYLSRYGFEESIRDGATMPLHFEPRLLDMHIDKEAIDQAFKELTGELSDL
                     DKDVLAKTAAKMAVLVKSPERIQKVCADIARHFQEKVAPSGFGAQVVTFDRESCVLYK
                     KALDEYLPPEVSDVVMTVNSGEDDYAPFRHERDAEEKLLDRFRDPADPLKMLIVTSKL
                     LTGFDAPILQAMYLDRPLRDHTLLQAICRTNRPYGEAKTHGLIVDYLGIFDDVAQAIQ
                     FDEEGITRVVTNIVELVMKLPEALQKCLAYFPGVDRAVTGYEGLIAAQACLPNNAARD
                     AFAADYSYLGRLWEAISPDPVLTQYESDFRWLTHVYESVKPSTGTGRLLWHVLGPKTI
                     ELIHQNVHVDAIRDDLETLVLDADLLEAVLGAPDPRGKAKEIEIKVARRLRKRMHDPR
                     FKALSERLEALKERHEQGLLLSIDFLKALLELARDVVAAEQTAEPIADESLGKAALTD
                     LFQEARNADTPVMVERIVDDIDEIVRYVRFDGWQSTHAGEREVKMALRKTLFKYKLHQ
                     DQELFGRAYGYVREYY"
BASE COUNT          467 a          891 c         1000 g          566 t
ORIGIN      
        1 ccggcgccgg gcgcacgaga aaaaccaaca gctcccagga agtcatccgc gttgcgacga
       61 ctgttcgccc ggaaaaggat acggacggtc gtgctcgtgt ccccgagggc gcagccaagg
      121 accagggtgt gcttcttatt ctggtgggtg ccacgcacca cggcaacggg gcttccgact
      181 gcaggaatcg ggcgacctct ccaacggcgc tctggaagtc atttgccaca gtcgcacgcc
      241 ctttccatac ggtcccctcg gccatcggaa ctagtgacac tatcgcagaa tggtgtgcgg
      301 cagctcagta atactcccgc acatacccat acgcccgccc gaagagctcc tgatcctggt
      361 ggagtttgta cttgaagagc gtcttgcgga gcgccatctt gacctcgcgc tccccggcgt
      421 gggtgctctg ccagccgtcg aagcgaacgt agcgcacaat ctcgtcgatg tcgtcgacga
      481 tgcgctccac catcaccggc gtgtcggcgt tgcgcgcctc ctggaagaga tccgtgagcg
      541 cggccttgcc cagcgactcg tcggcgatgg gctcggcggt ctgttcggcg gcgacgacgt
      601 cgcgggcgag ctcgagcaga gccttcagga agtcgatgct gagcagcagc ccctgctcgt
      661 gacgctcctt cagggcctcg agccgctcgc tgagggcctt gaagcgcggg tcgtgcatcc
      721 gtttgcgcag gcggcgggcc accttgatct cgatctcctt ggccttgccc ctcgggtcag
      781 gcgcgcccag taccgcttcg agcaggtcgg cgtcgagcac cagcgtctcg aggtcgtcgc
      841 ggatggcgtc cacgtggacg ttctggtgaa tcagctcgat ggtcttgggg cccagcacgt
      901 gccagagcag tctccctgtg ccggtggacg gcttcaccga ctcgtacacg tgggtcagcc
      961 agcggaagtc gctctcgtac tgggtcagca ccgggtcggg cgagatggcc tcccagagtc
     1021 ggcccaggta gctgtagtcg gcggcgaagg cgtcgcgggc ggcgttgttc ggcaggcagg
     1081 cctgggcggc gatgaggccc tcgtagccgg tgacggcgcg atcgaccccg ggaaagtagg
     1141 ccaggcactt ctgcagggcc tcgggtagct tcatcaccag ttccacgatg ttggtgacca
     1201 cccgggtgat gccttcctcg tcgaactgga tggcctgggc cacgtcgtcg aagatgccca
     1261 ggtagtccac gatcaggccg tgggtcttgg cctcgccgta ggggcggttg gtacgacaga
     1321 tcgcctgcag cagcgtgtgg tcacgcagtg gcctgtccag gtacatggcc tggaggatgg
     1381 gcgcgtcgaa gccggtcagc agcttcgagg tgacgatcag cattttcagc ggatcggcgg
     1441 ggtcgcggaa gcggtcgagg agcttctcct cggcatcccg ttcgtggcgg aagggagcgt
     1501 agtcgtcctc gccgctgttg accgtcatga cgacgtcgga gacctccggc ggcagatact
     1561 cgtcgagcgc cttcttatag agcacacagc tctcgcggtc gaaggtgacc acctgcgctc
     1621 cgaagccgct cggggccacc ttctcctgga agtgccgggc gatgtcggcg cagaccttct
     1681 ggatgcgctc cggggacttc accaacaccg ccatcttggc ggcggtcttc gccaacacat
     1741 ccttatcgag atcgctgagc tcgccggtga gttccttgaa ggcctggtcg atggcttcct
     1801 tgtcgatgtg catatcgagg aggcgcggct cgaagtgcag cggcatggtg gcgccgtcgc
     1861 ggatggactc ctcgaagccg tagcggctca ggtagccgga ggtatcctcg tcggcgccga
     1921 aggcgtagaa ggtgttgcga tcggcccggt tgatgggcgt gcctgtgagt ccgaagagga
     1981 aggcgttggg cagcgcgtcg cgcatcttcc ggcccaggtc gccctcctgc gtgcggtggg
     2041 cttcatcgac catggcgatg atgttggcac ggtcattgag gacaccttcg gcttcgccga
     2101 acttaaagat ggtggtgatg atgatcttgc gcacgtcctg ggcgagcagt tgcgcaagct
     2161 cggcccggct atcggccttg accaggttcg gcgtgtcggc ggcgtagaag gtgctggaga
     2221 tctgagtgtc gaggtcgatg cggtctacca cgatcatcac ggtggggttc ttgagggcgg
     2281 gatgcagccg gagcttgcgg gcggcgaaga gcatgaggag cgacttgccc gagccctgga
     2341 agtgccagat gagccccttc ttcgggtgcc cggcgaccac gcgctcgacg atcttgttcg
     2401 tgccctcgta ctgctgatag cgggcgataa tcttgatgcg gcgcttcttc gtgtcggtgg
     2461 cgtaggccgt gaagttggca agcaggtcga gaatgatgtt cggtcggagc atggaggcga
     2521 cggcctcctc gatccggtgc agggccgggg tcttttcgtc ggcgtcgacc caccacggcc
     2581 cccacatctc caccggcagc ccgatggacc cgtagtgaaa ttccttgcct tcggtcgcca
     2641 cggagagcag gttcggcacg aagagctcgg gtacgttgcg ctcgtagtcg tcgtgcacct
     2701 gaagcgcccc gtccagccag ctctggctgg cccggacagg cgtcttcgcc tccatcacca
     2761 caagcgggat gccgttgacg agcagcacca ggtcggtgcg cttctcagtg gctccggccc
     2821 ggatcgtaaa ttgggtggtg acgacgtatt cgttccgctc gaggtcggcg aagtcgatca
     2881 ggcggacggt gacgtgttcc ccactctcgc cgaagggcat ggac
//
LOCUS       DASRKH010000063         2081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_27180, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000063 DASRKH010000000
VERSION     DASRKH010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27180"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..496
                     /locus_tag="VFC06_01060"
     CDS             155..496
                     /locus_tag="VFC06_01060"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HZL80526.1"
                     /translation="MPSEGGLTGDTLGVGERIGYAQVPIEAHDPTTHRNALVALGVEP
                     DRVYVDEGQSATNRARPGLREAMAARQAGDTLVGARPDRLARARARERIAAARGKAGF
                     EQRVENLNFSP"
     gene            complement(814..>2081)
                     /locus_tag="VFC06_01065"
     CDS             complement(814..>2081)
                     /locus_tag="VFC06_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HZL80527.1"
                     /translation="RKPLAVHDPAKVITDLALTLALGGDCLADIALLRAEPGLFGRVA
                     SDATVSRTIDALAKDAPAALKAINTARAAARARAWSLAGTDAPDHHTSASTPLIVDLD
                     ATLVASHSEKELSRPNFKRGYGFHPLCSFIDHGPGGTGEPLAILLRPGNAGSNTAADH
                     LTVLKASLAQLPGHRPGTRPGRKVLIRIDGAGSTHAVLDWLTAQRLSYSVGFSLPAHT
                     PELLEHIPEHVWAPALDAHDEIRDGAWVADLTDLLALKNWPKGMRVIVRKERPHPGAQ
                     LTITDVDGHRITAFATNTRTGGPGTQLADLELRHRRRARCEDRIRIAKDTGLRAFPLF
                     DFAQNQIWCAIVALAVELTAWMQMLALHAHPARRWEPKRLRLRLFTVPATLARTGRRV
                     LLHLADKAPWAHLVGEGTTRLRALAAAAD"
BASE COUNT          303 a          650 c          802 g          326 t
ORIGIN      
        1 gtcgaagcca cgctgcttag gaaaactgtc ggcgacccag cggccgcgac tcgggagcgg
       61 cacgattgag gaacggccca cgggaacgat cgcgccgctc cgaacaagcc gcgcgaccta
      121 cctggatgaa ttcccataag acccgcctga gaaaatgccc agtgaagggg ggctaaccgg
      181 agacaccttg ggagtgggcg aacgtatcgg ctacgcacaa gtgccgatcg aggcgcacga
      241 ccccaccacc catcgcaacg cgctggtcgc gctcggtgtc gaacccgatc gggtgtatgt
      301 cgacgagggg cagtccgcca cgaatcgtgc ccggcccgga ctccgcgagg ccatggccgc
      361 ccgccaggcc ggggacacgc tcgtcggcgc cagacctgac cgcctcgccc gggctcgcgc
      421 ccgcgagcgt attgccgccg ccagggggaa ggccggattc gagcagcggg tcgagaactt
      481 gaacttctcc ccgtaggtac ggcaggccat cggtgacctg atcgatgtcg actgaccgaa
      541 acgcaagatc acacaggtcg ggactcctct tgcaccatgt gctgcgacgg gcctcggcag
      601 tcgctgaccg catcgtgtgg ccgggcatgg gcacccccga agcctaacct cgatctttca
      661 tgaggcggct gagctcggtc gcggcggtgt cgtagccgtc gtggtgggtt gattttggcc
      721 ctggggtctg acaagggccc gagtgtcgtc tcgggtgggc gccggttcca gttcgcgggg
      781 gtcgttcggg gttgtcggga tgggtcgcgg gtgtcagtcg gcggcggcgg ccagggcgcg
      841 tagtcgtgtg gttccttcgc cgaccaggtg cgcccagggc gctttgtcgg cgaggtggag
      901 caggacccgc cgcccggtgc gggccagggt cgccggcacg gtgaacaggc gcagccgcag
      961 ccgtttgggt tcccaccggc gtgcggggtg ggcgtgcagc gcgagcatct gcatccacgc
     1021 ggtgagctcg acggccagcg ccacgatcgc gcaccagatc tggttctggg cgaagtcgaa
     1081 cagcgggaac gcgcgcagcc cggtgtcctt ggcgatgcgg atgcggtcct cgcagcgggc
     1141 ccggcggcgg tggcgcagct cgaggtcagc gagctgggtg cccgggccgc cagtgcgggt
     1201 gttggtggcg aacgcggtga tgcggtgccc gtcgacgtcg gtgatggtca gttgcgcgcc
     1261 ggggtgcggt cgttccttgc gcacgatcac gcgcatgccc ttgggccagt tcttcagcgc
     1321 gagcaggtcg gtcaggtcgg cgacccacgc gccgtcgcgg atctcgtcgt gggcgtccag
     1381 cgcgggagcc cacacgtgtt cggggatgtg ttcgagcagt tcaggggtgt gggcgggcag
     1441 gctgaacccg acggagtacg acagtcgctg ggcggtgagc cagtccagga ccgcatgggt
     1501 cgacccggcg ccgtcgatgc ggatcagcac cttgcgcccg gggcgggtcc cgggccggtg
     1561 gccgggcagt tgcgcgaggg acgccttgag gaccgtgagg tggtcggcgg cggtgttcga
     1621 tcccgcgttg cccgggcgga gcaggatcgc caggggttca ccagtcccac cggggccgtg
     1681 gtcgatgaac gagcacaacg ggtggaagcc gtagccacgt ttgaagttcg gtcgagaaag
     1741 ttccttctcg gagtgggatg cgaccaacgt cgcgtccagg tccacgatga gcggggtgct
     1801 cgcgctggtg tggtggtcgg gggcgtcagt accggccagc gaccacgccc gggcccgcgc
     1861 ggcagcccgg gcggtgttga tggctttcaa cgcggcaggg gcgtccttcg cgagtgcgtc
     1921 gatcgtgcgg gacaccgtcg cgtcggaggc cacgcgtccg aacagtccgg gctcggcgcg
     1981 cagcagcgcg atgtcggcca agcagtcccc gcccagggcg agcgtgagcg cgaggtccgt
     2041 gatgactttc gcggggtcgt ggaccgcgag cggcttgcgc c
//
LOCUS       DASRKH010000064         2107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_22912, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000064 DASRKH010000000
VERSION     DASRKH010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22912"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1625
                     /gene="treS"
                     /locus_tag="VFC06_01070"
     CDS             <1..1625
                     /gene="treS"
                     /locus_tag="VFC06_01070"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HZL80528.1"
                     /translation="EVLIRSFRDSSGDGVGDLQGLTEKLDYIEWLGVDCLWLPPFFPS
                     PLKDGGYDVADYTAVAADIGTMDDFQEFLDAAHSRGIRVIIDVVMNYTSDQHPWFQAS
                     RSEPEGPFGDFYVWNDTDEPYPDARVIFVDTEPSNWSWDSVRQQYFWHRFYSHQPDLN
                     FENPAVQDTMIDVARFWLKHGVDGFRLDAVPYLFEAEGTNCENLPATHEFLRRIRRTV
                     DAEFPGTVILAEANQWPDDVVPYFGTEAEPECHMCFHFPVMPRLYHAVRDQRATPIID
                     ILADTPPIPRGAQWSTFLRNHDELTLEMVSTEERASMYGWFAPDSRMRANVGIRRRLA
                     TLLDNSRKELELLHALLLSLPGSPCIYYGDEIGMGDNIWLADRDAVRTPMQWTPDRNA
                     GFSTADPGKLYLPVVQSLVHHYGHVNVEAQLAQPTSLLHWVHGMLTVRRRHPTLARGD
                     FEVLTSSNDSVLAFRRYTADETILVVANLAATPRSTTVHLPGHAGAQLHDVFGGAPFP
                     AVGPDGTMLFTLGSRDFYWLALDRTSQPTELA"
     gene            1734..>2107
                     /locus_tag="VFC06_01075"
     CDS             1734..>2107
                     /locus_tag="VFC06_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="HZL80529.1"
                     /translation="MDARAVTREPRDDALLALLAEWLPGRRWFPAKGVAAELSCVGGL
                     TLVDPLDEADVRIVLVRTVAPGVDTVLQVPLTLHESGGARAGAAIGALGPDELSAAGS
                     ATADARTDVADGAGDPAFLRAWL"
BASE COUNT          320 a          773 c          687 g          327 t
ORIGIN      
        1 atgaagtact gatccgatcc ttccgcgact catcggggga cggggtgggc gatctgcagg
       61 gcctcaccga gaagctcgac tacatcgagt ggctcggagt ggactgcctc tggcttcctc
      121 cgttcttccc gtcgccgctc aaggacggcg gctacgacgt cgcggactac acggccgtcg
      181 ccgccgacat cggcaccatg gacgacttcc aggagttcct cgatgcggca cattcccgcg
      241 gcatccgggt gatcatcgac gtcgtcatga actacacctc ggatcaacac ccctggttcc
      301 aggcttcccg aagcgagccg gagggaccgt tcggcgactt ctatgtctgg aacgacaccg
      361 acgagcccta ccccgacgcg cgcgtcatct tcgttgacac cgagccatcg aactggtcct
      421 gggattcggt gcgccagcag tacttctggc accgcttcta tagccaccag cccgacctca
      481 acttcgagaa ccccgccgtg caggacacga tgatcgacgt cgcgcggttc tggctgaagc
      541 acggcgtcga cggtttccgg ctcgacgcgg tcccgtacct cttcgaggcc gagggcacca
      601 actgcgagaa ccttcccgcc acccacgagt tcttgcgccg gatccgacgg acggtcgacg
      661 cggagttccc cggcaccgtg atcctcgccg aggcgaacca gtggcctgac gacgtcgtgc
      721 cgtacttcgg caccgaggcc gagcccgagt gccacatgtg cttccacttc ccagtcatgc
      781 cgcgcctcta ccacgcggtc cgcgaccagc gcgccacgcc gatcatcgac atcctggcgg
      841 acaccccacc gatcccccgg ggcgcgcagt ggagcacgtt cctgcgcaac cacgacgagc
      901 tgacgctcga gatggtctcg accgaggaac gcgcgtcgat gtacggctgg ttcgcgcccg
      961 actcgcgcat gcgcgccaac gtcgggatcc gacgtcggct cgcgactctc ctcgacaact
     1021 cccgcaagga gctcgagctc ctgcatgcgc tgctcctctc gctgccgggc agcccgtgca
     1081 tctactacgg cgacgagatc gggatgggcg acaacatctg gctcgcggac cgggacgccg
     1141 tccgcacccc gatgcagtgg accccggacc gcaacgccgg cttctcgacg gcggaccccg
     1201 gcaagctgta cctgccggtg gtccagtcgc tcgtgcacca ctacggccac gtcaacgtgg
     1261 aggcacagct cgcgcagccg acgtcgttgc tgcactgggt ccacggcatg ctcacggtgc
     1321 ggcgccgtca cccgactctg gcgcgaggtg acttcgaggt gctgaccagc tccaacgact
     1381 cggtcctcgc gttccggcgg tacaccgcgg acgagacgat cctcgtggtc gcgaacctgg
     1441 ccgcgacacc acggtcgacc accgtgcacc tgccggggca tgcgggcgca cagctgcacg
     1501 acgtcttcgg cggagcgccg ttccccgcgg tcgggccgga cggcacgatg ctgttcacgt
     1561 tggggtcgcg cgacttctac tggctcgccc tggaccggac ctcgcagccg accgagctgg
     1621 catgagcccg acggccgcgc gacgatgcgg ccccagccac cgtgcgctcg gcgcgtcgtg
     1681 cacccatgct gtcgggagca cgacgccgtg agcactggag ggacctgagc gccatggacg
     1741 cacgagcggt gacgcgcgag ccgcgggatg acgcgctgct ggcgctgctc gccgagtggc
     1801 tgcccggccg acggtggttc ccggccaagg gcgtcgctgc cgagctgtcc tgcgtgggcg
     1861 gtctgaccct ggtcgacccc ctggacgagg ccgacgtgcg gatcgtgctc gtgcggaccg
     1921 tcgcgcccgg tgtcgacacc gtcctgcagg tcccgctgac gctgcacgag tcgggcggcg
     1981 cccgtgccgg cgcagcgatc ggcgcactcg ggcccgacga gctgtcggcc gcgggctctg
     2041 ccaccgcgga cgcgcggacc gacgtcgccg acggagccgg cgacccggcg ttcctgcgcg
     2101 cgtggct
//
LOCUS       DASRKH010000065         2137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_51751, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000065 DASRKH010000000
VERSION     DASRKH010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51751"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..294)
                     /locus_tag="VFC06_01080"
     tRNA            complement(207..294)
                     /locus_tag="VFC06_01080"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(258..260),aa:Ser,seq:gct)
     gene            complement(381..845)
                     /locus_tag="VFC06_01085"
     CDS             complement(381..845)
                     /locus_tag="VFC06_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80530.1"
                     /translation="MRFQQFADDEGLPSLQSMFVLALRMYMAKVNGRTDDVMVGVNVG
                     RRGSREERLSGGSRAQHLRFRTILPPEMTFLEALRVLAERQSTFYRHVDFPCYLLVTD
                     DDGSGALRCYWERNIRHVPVETIERCHDFMVRAMRAGVERPTITLGEIMDLP"
     gene            868..1224
                     /locus_tag="VFC06_01090"
     CDS             868..1224
                     /locus_tag="VFC06_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80531.1"
                     /translation="MFLIHAALTSGAPMPEPILPYEEMLQTELDYVGSPRQAKDRAFW
                     QAELEGFGGPPAYSNIDGTHARDRYRKLIREPDHPFGSVVFLSTVASTTSASTSSWHA
                     AGPSARRGRAATGRRA"
     gene            1255..1548
                     /locus_tag="VFC06_01095"
     CDS             1255..1548
                     /locus_tag="VFC06_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80532.1"
                     /translation="MVTFTMIAFDVGGDLPFRAKGYCTGTTPMPVYVILSTGDTPGSL
                     RVIYEYQHKRIDVAALHQCHAAMLDVIRTGIRNPSITLRELLDLPEPAADAAR"
     gene            complement(1586..>2137)
                     /locus_tag="VFC06_01100"
     CDS             complement(1586..>2137)
                     /locus_tag="VFC06_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80533.1"
                     /translation="DQQSAWTSDYDTALNDPAGANGDPANVPAGDYGPVPTLATGLVA
                     MGASGALDGVLAAPGQFYNTDSTKQILFMGDGGYLDAAGTANHLQGNQWGMMNETGNY
                     PGQQWLAPASVWYQLPIFNSDAESGLAATLTANGDIYIMSIVGIITLAMILLPFIPGL
                     RGIPRLIPIHKLIWRDYYNRNTS"
BASE COUNT          377 a          695 c          706 g          359 t
ORIGIN      
        1 gtcccatgga cccacgcacc gatcccgggg acaggagggc ggtcaggcag gaccaaagtc
       61 ccgccgcccc tgctggcgat cggagcacaa tcaaggccca ctactctttg tataccaccg
      121 ttgagatgcc ccacagtcgc atatgtgaat gactggtgaa gtagcgaaca tcacgttcgc
      181 aagagcggtt gttaggttga caagagcgga gacggtggga tttgaaccca cggaagggga
      241 gtccccttca cggccttagc agggccgcgc actaggccac tatgcgacgt ctcccagaac
      301 cccctcaggg taccgcgagt gcccaatcgc ggccaaacag cggaaccccg tggaggccaa
      361 cggcctcgaa cccccaaccc tcagggcagg tccatgatct cgccgagggt gatcgtcggc
      421 cgctccaccc cggcgcgcat cgcgcgcacc atgaagtcgt ggcacctctc gatcgtttcg
      481 accggtacgt gcctgatgtt ccgctcccag taacagcgca aggcaccgga accgtcgtcg
      541 tccgtgacga ggagataaca ggggaagtcg acgtgccggt agaaggtact ctgcctctcg
      601 gccaggacgc gcagggcctc gaggaaggtc atctccgggg ggaggatcgt gcgaaagcgc
      661 aggtgctgcg cccgggaacc cccggacagc ctctcctcgc gtgaacctcg gcggcccacg
      721 ttcaccccga ccatgacgtc atcggtccga ccgttcacct tcgccatgta catgcggagt
      781 gcgagcacga acatggactg cagggacggg agaccctcgt cgtccgcgaa ctgctggaac
      841 cgcactatca acctcttcta tcgcgacgtg ttcctgatcc acgcggccct caccagtggc
      901 gcccccatgc ccgagccgat cctgccctac gaggagatgc tccagacgga gctcgactat
      961 gtcggttcac cccggcaggc caaggacagg gcgttctggc aggcggagct cgaggggttc
     1021 ggcgggcccc cggcctacag caacatcgac gggacccacg cgcgggaccg ctaccgcaag
     1081 ctgatcaggg agccggacca cccattcggc tccgtggtat tcctgagcac ggtcgcgtcg
     1141 acgacgtcag cctcaacgtc gtcgtggcac gccgcgggac cctccgcgag aagaggacgg
     1201 gcggcgaccg ggcgacgggc atgatcctgc ggacagtcct cgaacccgaa cgccatggtg
     1261 accttcacca tgatcgcctt cgacgtgggc ggcgacctgc ccttccgcgc gaaggggtac
     1321 tgcacgggca ccacgccgat gccggtttac gtgatcctct cgacgggtga cacgccgggg
     1381 tcgttgaggg tcatctacga gtaccagcac aagcgcatcg atgtggcggc gctgcaccag
     1441 tgccacgccg ccatgctcga cgtgatccgc accgggatcc ggaatccgtc gatcacgtta
     1501 cgggaactgc tcgacctccc cgagccggcg gccgacgccg cccggtgacc gacggtgccg
     1561 ccgactctct gtctcccaat ccgggtcagg aggtattcct gttgtagtag tcgcgccaga
     1621 tgagcttgtg gatggggatc aggcgcggga tccctcggag gccggggata aagggtagga
     1681 ggatcatcgc cagggtgatg atgccgacga tggacatgat gtagatgtcg ccgttggctg
     1741 tgagggttgc ggccaggccg ctttcggcgt cgctgttgaa gatcggcagc tggtaccaga
     1801 cggaggccgg tgcgagccat tgctggcccg ggtagttgcc ggtctcgttc atcatgcccc
     1861 actggttgcc ctggaggtgg ttggcggtgc cggccgcgtc gaggtagccg ccgtcgccca
     1921 tgaagaggat ctgcttcgtg ctgtccgtgt tgtagaactg ccccggcgcc gcgagaacgc
     1981 cgtcgagcgc acccgaggct cccatcgcaa ccaggccggt ggcgagggtt ggcacgggtc
     2041 cgtagtcgcc agcggggacg ttggccgggt cgccgttggc tcctgccggg tcgttgagtg
     2101 cggtgtcgta gtcgctagtc catgcgctct gctggtc
//
LOCUS       DASRKH010000066         2291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_23780, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000066 DASRKH010000000
VERSION     DASRKH010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23780"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VFC06_01105"
     CDS             complement(<1..233)
                     /locus_tag="VFC06_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80534.1"
                     /translation="MRRIPITMALIASTALALSACGGGTTDGGTTAATDAATAGGATT
                     ATGSDGAVYVFLPKSLNNPYWVDARKGMEDAACL"
     gene            complement(383..1717)
                     /locus_tag="VFC06_01110"
     CDS             complement(383..1717)
                     /locus_tag="VFC06_01110"
                     /inference="COORDINATES: protein motif:HMM:NF018682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HZL80535.1"
                     /translation="MLDDDPTGSQSVQGIPELTRWEDEDLRWAFDQPTDGFFILTNTR
                     GLNEDEARATVLDVARAVRRVSDQLGVSCSLIARGDSTLRGHYPMETDALIELAAASG
                     APYDALLLAPAYLAAGRVTSGDVQYVRDGESYVPVGLTNYARDATFGFRSSNIRHYVE
                     EKTHAAIAADDVQSLSLEDIRVGGVERVRDVLLGCRNAVPVIVNAVDDSDLDVVVLGL
                     IEAEKAGVRVLSRTGPSFVAARLGLASRPPLRHSEIYPNGERPGHGLVIVGSHVELTT
                     RQVDRLVSEVPDLAIVELAVPLLLDATQARAELDRCGAALISALEQADTMLVTSREQI
                     VGETGHSSLLIAQTVSAALSALTARAVREVPLTWVVAKGGITSSDVATEGLSIRRATV
                     VGQLFPGIVSVWVHEGGNGTSLDGLPYVVFAGNVGDESTLASAVRIMRGELR"
     gene            complement(1849..>2291)
                     /locus_tag="VFC06_01115"
     CDS             complement(1849..>2291)
                     /locus_tag="VFC06_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018762773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HZL80536.1"
                     /translation="SSLGPALDVAARAVNIPVVKIDEAMAIRAALEGKRIGVLATVPT
                     TLAPTSELIRSKAIELGRAVEIEQRLCAGAFDILVAGDREKHDAMVTEQAVDLAKGVD
                     IIVLAQASMSRLASSLGEITGLPVLSSPRIGVEYLAERVAALQP"
BASE COUNT          384 a          769 c          739 g          399 t
ORIGIN      
        1 agacaggcag cgtcctccat gcccttgcgg gcgtcaaccc agtacgggtt gttgagcgac
       61 ttcggcagga agacatagac ggcgccgtcc gagccggtcg cagttgtggc cccgccggcc
      121 gtggcggcat cggttgcggc ggtcgtgccg ccatcggtcg tgccaccacc gcaggcgctg
      181 agcgccaacg ctgtggaggc gatcagggcc atcgtgatag gaattcgtcg cattgctgta
      241 ctcctttgta ttggctgcac cttctgatgc gtgatatttc ggggtggttg ggaatacgcg
      301 catctctact tataggcact ctccttgccc gtaccggacc gcactcccgc ccgccgtgcg
      361 cgtgctgcgg ctttgctgtt gatcaccgga gttcaccccg catgatccgc accgccgacg
      421 cgagagtgga ttcgtccccc acattccccg cgaacaccac atagggaagc ccgtcgagac
      481 tcgtcccgtt gcccccctcg tggacccaga cggagacgat gccggggaac agttgaccga
      541 cgaccgttgc ccgtcgaatc gacaacccct cggtggcgac gtcgcttgag gtgattccac
      601 ctttcgcgac cacccatgtg aggggaacct cgcgaacggc ccgtgccgtg agcgccgaga
      661 gcgccgcgga caccgtctgc gctatcagaa ggctcgagtg accggtctcc cccacaatct
      721 gttcgcggct cgtgacaagc atcgtgtccg cctgctcaag cgccgagatc agcgccgcgc
      781 cacaccgatc cagttccgcg cgcgcttgcg tggcgtcaag gagcagtggc actgcgagtt
      841 cgacgatcgc caggtcaggg acttcgctga cgagccgatc aacctgacgg gtggtgagtt
      901 cgacgtgcga tcccacgatc acgaggccgt ggcccggacg ttcgccgttc ggatagattt
      961 cggagtgcct cagtggtggc cgcgacgcaa gcccgagccg tgccgcgacg aacgacgggc
     1021 ccgtccggct gagcacgcgg acgcctgcct tctccgcctc gatcagtccg aggacgacga
     1081 catccaggtc ggagtcgtcc acagcgttga cgatgacggg caccgcattt cggcagccga
     1141 ggagcacatc gcgcacacgt tcgaccccac cgacgcgaat gtcctcgaga ctcaggctct
     1201 ggacgtcgtc cgccgcgatt gccgcgtggg tcttctcctc gacgtagtgc ctgatgttgg
     1261 aactccggaa cccgaatgtg gcgtcccggg cgtaattcgt tagcccgaca ggcacgtagc
     1321 tctcgccgtc ccggacgtac tgcacatcgc cggacgtgac acgtcccgca gccaaatacg
     1381 ccggggcgag gaggagggcg tcgtagggcg cgccgctggc tgccgcaagc tcgatgagag
     1441 catccgtctc catggggtaa tgcccccgca gggtggagtc gccgcgggcg atgaggctac
     1501 aactgactcc cagttgatcg ctcacgcgtc ggaccgcccg ggccacatcg aggacggtgg
     1561 cgcgagcttc gtcctcgttc agtccgcggg tgttggtgag gatgaagaat ccatcggtgg
     1621 gctggtcgaa tgcccagcgc aggtcctcgt cctcccaccg ggtgagttct gggatcccct
     1681 gcaccgactg gctcccggtc gggtcgtcat ccagcacaac gattcgcctc ttgacaacgg
     1741 cattcgctgc gcgaatacgc gcccggaggt cgccatgttt ggggaccgcg ggaccagcgg
     1801 cgagcagctc ctcaccccgt ctgatcggcg agggaaaatc gggcccgttc acggctgcag
     1861 cgccgcaacg cgctcggcga ggtactcgac gccgatgcgg gggctcgaga gaaccgggag
     1921 gcccgttatc tctcccaagg acgaggcgag gcgcgacatg gaggcctgcg cgagcacgat
     1981 gatatcgacg cccttggcga gatccaccgc ctgttccgtg accatcgcgt cgtgcttctc
     2041 acggtcgccg gccacgagga tgtcgaacgc gccggcacac aagcgctgtt cgatctcgac
     2101 ggcacggccg agttcgatcg ccttcgaccg gatcagctcc gacgtcggcg cgagtgtcgt
     2161 cggaacggtg gccagaactc cgattcgctt gccctcgagt gccgcccgga tcgccattgc
     2221 ctcgtcgatc ttgacgaccg ggatgttcac ggcccgggcc gcgacatcca gcgctggacc
     2281 gagcgatgaa c
//
LOCUS       DASRKH010000067         3118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_1273, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000067 DASRKH010000000
VERSION     DASRKH010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1273"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /gene="cydB"
                     /locus_tag="VFC06_01120"
     CDS             <1..899
                     /gene="cydB"
                     /locus_tag="VFC06_01120"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HZL80537.1"
                     /translation="ATFAAFPEWYATMFSGMYLALFLILVALIVRILALEWRSKISDV
                     TWRQRWDAVHIVAAWIPAVLWGVAFANLVQGMEIEVVGRDGVVVAPDAVAGALGEASH
                     QLTGGFFSLLTQFTLLGGVVTATLFLTSGAVFIALKTEGDLRERAGDLAARLSVLSAG
                     VTAVFALWGQLVYARSAIGWLPLVIAAVCLVAVVLTTRMRREGWSFLLNSVGIAAAVA
                     WIFVAMHPYVMPSTIDDAYSLSIVQASSTPYTLKVMAIVALTLVPVILAYQAWTYWVF
                     RKRISTRHIPLAAAGLELAETR"
     gene            933..2612
                     /gene="cydD"
                     /locus_tag="VFC06_01125"
     CDS             933..2612
                     /gene="cydD"
                     /locus_tag="VFC06_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydD"
                     /protein_id="HZL80538.1"
                     /translation="MTPLDPRLLRHARASRGYILTTTVTGVVLTGLVVAQALLVSRII
                     APVIEGTAGFPDVRGPVAALAAVLAMRVTVLVTQERFAHRAATAVIAELRGKVLDHAV
                     AQGPRWLDATRSAEIVTLVTRGLDDLAPYFVRYLPQLLLAATLTPATLAVVFSLDLTS
                     GLILTGALPMIPLFMWLVGRLTESYTEQRLAMMDRLGGQLLDLLAGLTTLKALGRERG
                     PGARVRLLAEAYNATTMASLRVAFLSGAILEFIASLSVALVAVVVGMRLVFGDLDLTT
                     ALAAIMLAPEVLQPLRQVGAQFHASANGVAAATAAFGVLERPAPAVGTRPAPPLGRAT
                     IEFRDVSVRAPGRDVLAPAHLDATIAPGTITALVGATGAGKSTSALLLLGLLRPDGGQ
                     VLVHHDGEVTDLSLLDPATLHHQMTWIPQRPTILPGTIIQNVLGEFDGDPSPTPALER
                     AAVLSGFAGTVAALPGGWRTAIGHGGVGLSVGQRQRLALTRTLVESSPLVVLDEPTAH
                     LDAATEENVIAAVRELRAQGRTVVVIAHRPSMIEAATHTVRVSSASLVGEP"
     gene            2609..>3118
                     /locus_tag="VFC06_01130"
     CDS             2609..>3118
                     /locus_tag="VFC06_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HZL80539.1"
                     /translation="MSVLSSGQRSALRRAVRLLDLDQRRVLLAVLAGSLGLGSSVGLT
                     ATSAWLIARASQMPAVLDLTVASVGVRTFGISRSVLRYFERLVSHDIALRGMASLREQ
                     VYRRLADSPVEAVAGLRRGDLLVRTGADVDAVGDVVVRALLPAGVAVVVGTGTVGPTV
                     PASSRHRASN"
BASE COUNT          351 a         1198 c         1075 g          494 t
ORIGIN      
        1 gcgctacgtt cgccgccttc cccgagtggt acgccacgat gttctcgggc atgtacctcg
       61 cgctgttcct catcctcgtg gcgctcatcg tgcgcatcct ggcgctcgag tggcgctcca
      121 agatctctga cgtgacctgg cggcagcgct gggacgccgt ccacatcgtc gccgcgtgga
      181 tcccggcggt cctctggggt gtcgcgttcg ccaacctggt ccagggcatg gagatcgagg
      241 tcgtgggccg cgacggcgtc gtcgtggccc cggacgccgt tgcgggtgcg ctcggtgagg
      301 catcccacca gctcaccggc gggttcttct cgctcctcac ccagttcacc ctgctgggcg
      361 gtgtggtcac ggcgacgctg ttcctgacgt cgggcgcggt gttcatcgcg ctcaagacgg
      421 agggcgacct gcgcgagcgg gccggcgacc tggcggccag gctctcggtg ctcagcgccg
      481 gggtcaccgc ggtgttcgcg ctctgggggc agctggtgta cgcccgcagc gcgatcgggt
      541 ggctccccct cgtgatcgcc gcggtgtgcc tagtggccgt ggtcctcacc acgcggatgc
      601 gccgggaggg ctggtccttc ctgctcaact ccgtggggat tgccgccgcg gtggcgtgga
      661 tcttcgtggc aatgcacccg tacgtgatgc cgtccacgat cgacgacgcg tactcgctgt
      721 ccatcgtgca ggcgtcctcg accccctaca cgctcaaggt gatggcgatc gtcgccctca
      781 cgctcgtccc ggtcatcctc gcctaccagg cctggaccta ctgggtgttc cggaagcgca
      841 tctccacccg gcacattccc ctcgcggcgg caggactgga gcttgccgag acccgctgac
      901 gcccgcggcg ccgcgggtga cagagtggag ccatgacccc actggaccca cgcctgctgc
      961 ggcacgcgcg agcctcacgc ggctacatcc tgaccaccac ggtcaccggc gtcgtcctga
     1021 cgggtctcgt ggtggcccag gccctgctgg tctcgcggat catcgcaccg gtgatcgagg
     1081 ggacggccgg cttcccggac gtccgcggcc cggtcgccgc gctggccgcc gtcctcgcca
     1141 tgcgcgtgac ggtgctcgtg acgcaggagc ggttcgccca ccgggccgcc actgccgtca
     1201 tcgccgaact gcgcgggaag gtgctggacc acgccgtcgc ccagggcccg cggtggctcg
     1261 acgccacccg gtccgcggag atcgtgacgc tggtgacgcg cggactggac gacctggccc
     1321 cctacttcgt gcggtacctc ccccaactgc tcctcgccgc caccctcacg cccgcaacgc
     1381 tcgccgtggt gttctcgctc gacctcacct ccgggctgat cctcaccggg gcgctgccga
     1441 tgatccccct cttcatgtgg ctggtgggcc gcctcacgga gagctacacc gagcagcggc
     1501 tcgccatgat ggaccgcctc gggggccagc tgctggacct cctggcgggc ctgaccaccc
     1561 tcaaagctct cggccgcgag cgcggcccgg gcgcccgggt ccgccttctc gctgaggcgt
     1621 acaacgccac cacgatggca tcgctgcgcg tggccttcct ctccggtgcc atcctcgagt
     1681 tcatcgcgag cctctcggtg gcgctggtgg ccgtggtggt cggcatgcgc ctcgtgttcg
     1741 gcgacctgga cctgaccacc gcgctggccg ccatcatgct tgcgcccgaa gtgctccagc
     1801 cgctgcgcca ggtgggcgcg cagttccacg ccagcgccaa cggtgtggcc gccgcgaccg
     1861 cggccttcgg cgtcctggag cgccccgccc ccgcggtggg gacgcgcccg gcgccgccgc
     1921 tgggacgcgc gacgatcgag ttccgcgacg tcagcgtgcg cgccccgggg cgcgatgtgc
     1981 tcgcccccgc ccacctcgac gccaccatcg cgcccgggac catcaccgcg ctcgtggggg
     2041 cgaccggggc cgggaagtcc accagcgccc tgctcctcct cgggctgctg cgccccgacg
     2101 gcgggcaggt cctcgtgcac cacgacgggg aggtcaccga cctctccctg ctcgacccgg
     2161 ccacgctcca ccaccagatg acctggatcc cccagcggcc caccatcctg ccgggcacca
     2221 tcatccagaa cgtcctcggc gagttcgacg gcgatccctc ccccaccccc gcactggaga
     2281 gggcggccgt gctcagcggg ttcgccggga ccgtcgccgc gctcccgggc ggctggcgga
     2341 ccgctatcgg gcacgggggc gtcggcctga gcgtgggcca gcgccagcgc ctcgccctga
     2401 cgcgcacact cgtggagtcc tcgccgctgg tggtgctcga cgagccgacg gcgcacctgg
     2461 acgccgccac cgaggagaac gtgatcgccg cggtgcgcga gttgcgggcg cagggccgga
     2521 ccgtggtggt gatcgcccac cgcccttcca tgatcgaggc cgccacgcac acagtccggg
     2581 tctcctcagc ctccttggtg ggcgagccat gagcgtgctc tcctccggcc agcgcagcgc
     2641 gctgcgccgc gccgtccgcc tcctcgacct cgaccagcgc cgcgtgctgc tcgccgtcct
     2701 cgccggctcg ctgggcctcg gctcgtccgt ggggctcacc gcgacctccg cgtggctgat
     2761 cgcgcgggcc tcccagatgc ccgccgtcct cgacctcact gtggcgtccg tgggggtgcg
     2821 caccttcggg atctcccgct ccgtgctgcg gtacttcgaa cgcctcgtct cgcacgacat
     2881 cgccctgcgc gggatggcgt cgctgcgcga gcaggtgtac cgccggctcg cggactcgcc
     2941 cgtggaggcc gtggcgggcc tccggcgcgg ggacctgctg gtgcggaccg gcgcggacgt
     3001 ggacgcggtg ggcgacgtcg tggtgcgggc gctgctgccc gcgggggtcg ccgtcgtggt
     3061 ggggaccggg accgtcgggc cgacggtccc ggcaagcagc aggcacaggg ccagcaac
//
LOCUS       DASRKH010000068         2015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_16122, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000068 DASRKH010000000
VERSION     DASRKH010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16122"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="VFC06_01135"
     CDS             <1..891
                     /locus_tag="VFC06_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80540.1"
                     /translation="IADPTAQVTFVMDGDDAGLAATSRLSWAAEALGVRGFGILLDPD
                     EGKDPWDRYTKGTLAADLNESSQRPLTEACLSAVKSVVKDDIEFDAAIAALIQAARTP
                     DHSDALLRAASKVRKVTVGEYGETVRRQTLAKGGRARANALRVDLSPSVRHLVARMLQ
                     VPPRELMAIASTFGGWDDQSQGFAKRWLPTKGLVDVAAIGYTLFPHAEHYDAEVVEAV
                     SSCMSQDDTVESIEPILRGLGRVVLAEAARSSRDAPASILTRVRAVRSLASSGVKTED
                     EPAYLTLLLDVARELDSVES"
     gene            complement(888..>2015)
                     /locus_tag="VFC06_01140"
     CDS             complement(888..>2015)
                     /locus_tag="VFC06_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80541.1"
                     /translation="SAQALPRDVQAAPQDSHETIPTTRPESKRLVATLRAAAASAVRE
                     WRAKGAEPTQHMMFDMDVYRDEVIDLLDIHIETTRDAEESLASRGLGSATAGSSEHAE
                     RLCYHAIRTVKGLAIHPSVMARILARADIGSGYDSRDFTKFAIGLRAVAHTNPAVRRV
                     LSKPAEDVDPSDLNAVAALLRNFAATRSGRVRLDEAGRARSEVLREHLAGFGPDAVTE
                     VNETIERIHAAAATHGEFSPLSASDDCAGLLALPSVVAGDLLAHGLSYAQWRDLGWSQ
                     YAGDSPLMLLEPALVAMRRDGMPDEMILAIRPDVGSDDTEYPYSVSRFLAGPGAYAVG
                     GQSERGVEAFGSGVITHEAEQIRERFERDTRGRKTNRLPGV"
BASE COUNT          322 a          662 c          696 g          335 t
ORIGIN      
        1 atcgccgacc ccacggcgca ggtcacattc gtcatggacg gcgacgacgc ggggcttgcc
       61 gccacatctc gcctgtcgtg ggcggcagaa gcgcttggcg tccgcggctt cggaatcctc
      121 ctggatcccg acgagggcaa agacccctgg gaccgctaca cgaagggaac cctggccgca
      181 gaccttaacg agtcgagcca gcgcccgctc accgaggcct gcctgtctgc cgtgaagtcc
      241 gtcgtcaagg acgacataga gttcgacgcc gccattgcag cgctcatcca agccgctcga
      301 acccccgacc actcggacgc gctgcttcgc gcagcgtcca aggtcaggaa ggtgaccgtg
      361 ggcgagtacg gcgagacagt gcgccgccaa acgctcgcca agggtggtcg cgcacgcgcc
      421 aatgcgctgc gggtcgacct ctccccctcc gtgaggcacc tcgtcgcacg catgttgcag
      481 gtgccacccc gcgagttgat ggcgatcgcc tcgacgttcg gcggctggga cgaccagtcg
      541 cagggctttg ccaagcgctg gctgcccacg aagggactcg tcgacgtggc agccattggg
      601 tacacgctct ttccgcacgc tgagcactac gacgccgagg tcgtcgaagc ggtctcctcg
      661 tgcatgtccc aggacgacac tgtcgagtcc atagagccca ttctccgggg cctgggccgc
      721 gtggtgctgg cagaggccgc acggtcgtcc agagacgctc ctgcgtccat cctgacgcgc
      781 gtgagggctg ttcggtcgct ggcatccagc ggagtcaaga ccgaggatga gcccgcgtac
      841 ctgacgctgc tgctcgatgt cgctcgggaa ctcgactcgg tcgagtccta gacgccggga
      901 agccggttcg tcttgcgtcc gcgggtgtcg cgctcgaacc gctcacggat ctgttccgct
      961 tcgtgcgtga tgacgccgga gccgaacgct tcaacgcccc gttcgctttg gccgccgacg
     1021 gcgtatgcgc cggggcctgc caggaatcgt gaaacggagt acggatactc ggtgtcgtcc
     1081 gaccccacat ctggccgaat cgcgaggatc atctcgtcag gcattccgtc gcgacgcatt
     1141 gcgaccagcg ccggctcgag cagcatgagg ggcgagtctc ctgcgtactg agaccaccca
     1201 agatcgcgcc actgggcata cgagagcccg tgcgcgagga ggtccccggc cacaacggac
     1261 ggcagggcga gtagccccgc acagtcgtcg gacgccgaca gcggcgagaa ctctccgtga
     1321 gtggccgcgg cagcgtggat ccgctcgatc gtttcgttga cctcggtgac tgcgtccgga
     1381 ccgaagccgg caaggtgctc gcgaagcact tcgcttctcg cgcggcccgc ctcgtcgagt
     1441 cgtacgcggc cagagcgtgt ggcggcgaaa ttgcgcagga gcgccgcgac ggcgttgagg
     1501 tcgctcgggt cgacgtcctc tgcgggcttg gacagcacgc ggcggactgc ggggttggtg
     1561 tgagcgacgg cgcggaggcc gatcgcgaac ttggtgaagt cgcgggagtc gtagccggag
     1621 ccaatatctg ccctcgcgag aatgcgagcc atgacagacg ggtggatggc gaggcccttg
     1681 actgtcctga tggcgtggta gcacagccgt tcggcgtgct cgctggaccc tgcggtagcg
     1741 gacccgagac cgcgcgaggc cagcgactcc tcggcgtctc gcgtggtctc tatgtggata
     1801 tcgaggaggt cgatgacctc gtcgcggtac acgtccatgt cgaacatcat gtgctgggtg
     1861 ggctccgcgc ccttggcgcg ccactcgcgg accgccgagg cggccgctgc acgcagtgtg
     1921 gcaacgagcc gtttggactc gggacgcgtc gttggaatgg tctcgtgcga gtcctgcggg
     1981 gccgcctgga cgtcgcgtgg cagcgcctgg gcaga
//
LOCUS       DASRKH010000069         2212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_21268, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000069 DASRKH010000000
VERSION     DASRKH010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21268"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1232
                     /locus_tag="VFC06_01145"
     CDS             <1..1232
                     /locus_tag="VFC06_01145"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HZL80542.1"
                     /translation="QVLSQAETNLPLPPVPRSVTVNGDWTYSPVLDEVTSDTATTQNL
                     SYQFDADFNYFSVDGLKALGTASAQDALLDPGYLAIADAIDVDKVRASAQEIVGDAAT
                     RYDQAIALQEFFRNTQDFTYDTSVAPSGDDSVSVFLKNRAGYCVQFASALVVMSRSLG
                     IPARLAIGFLPGERGADGESVITGGDAHAWPELYFAGAGWVRFEPTPAVQTGARPAYA
                     DPAGADSQTPFNEFQVPRQGPTIAPSAAPVTTGIPGVTEGANDSAAPPWWLWAVVVMV
                     LAGLAVGWRMVRSRHVAVARVDNPEHVWTWLRERLPREHSWPDSLTPHESEASLRHSL
                     FQETFFLSPDADRALSDVVVAVSDHRYAPEGSTQSIQDLRDSARIVADEIAATGSNDA
                     RGRPSRAGAPDVPRRDA"
     gene            complement(1177..1557)
                     /locus_tag="VFC06_01150"
     CDS             complement(1177..1557)
                     /locus_tag="VFC06_01150"
                     /inference="COORDINATES: protein motif:HMM:NF022684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3040 domain-containing protein"
                     /protein_id="HZL80543.1"
                     /translation="MMPLSEYEQRVLDQLERDLGTDPKLGRTMARGYRQRGRIVGGAL
                     GVLIGLGVVLIGAVAQIIPVGIAGFALMLAAAMWAMFTPRKHVAAKASGRGATQAAKK
                     QADKESFMRRVEERLARRREKGDL"
     gene            complement(1750..1953)
                     /locus_tag="VFC06_01155"
     CDS             complement(1750..1953)
                     /locus_tag="VFC06_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HZL80544.1"
                     /translation="MAQGSVKWFNAEKGYGFIAQDGGGADVFVHYSAIITDGFKSLNE
                     AQRVEFEVTQGPKGPQAEDVRAL"
BASE COUNT          381 a          744 c          684 g          403 t
ORIGIN      
        1 tgcaggtgct ctcgcaggcg gagacgaacc tacctctccc gccagttccg cgttccgtga
       61 cggtgaacgg ggactggacc tactcccccg tgctcgacga ggtcacctct gacaccgcca
      121 ccactcaaaa tctcagctac cagttcgatg cggacttcaa ctacttcagc gtcgacggat
      181 tgaaggcctt ggggaccgcg tccgcgcagg acgctctgct cgatccgggc tacctggcga
      241 tcgccgacgc catcgacgtg gacaaggtgc gtgcgtcggc acaagagatc gtcggcgatg
      301 cggccacccg gtatgaccag gcgatcgccc tgcaggagtt cttccgcaac acccaggact
      361 tcacgtacga cacctccgtg gccccctctg gcgacgactc ggtatcggtg ttcctcaaga
      421 accgtgcggg ctattgcgtg cagttcgcgt cggcactcgt cgtgatgtcc cgctcgctcg
      481 gcatccccgc acgacttgcg atcggcttcc ttccgggcga acgaggcgcc gacggcgagt
      541 ccgtgatcac gggcggcgac gcgcatgcgt ggcccgagct gtacttcgcg ggagccggtt
      601 gggtgcgctt tgaaccgacg cctgccgtgc agacgggcgc gcggccggcc tacgccgacc
      661 ccgcgggcgc ggattcgcag actccgttca acgagttcca agtgccgcga caggggccca
      721 ccatcgcgcc cagcgcggcg cccgtgacga ccgggattcc tggagtgact gagggggcca
      781 acgattcggc ggcgccacca tggtggctgt gggcggtcgt ggtcatggtt ctcgccggcc
      841 tcgcggtcgg gtggcgaatg gtacggtctc gccacgtcgc ggtggcgcgc gtggacaatc
      901 ctgaacacgt gtggacgtgg ttgcgcgagc gacttccgcg cgagcactca tggcccgaca
      961 gcttgacgcc gcacgagagc gaggcgtcct tgcgtcacag cctgttccag gagacgtttt
     1021 tcctgtctcc ggacgcggat cgcgcgctgt cagacgtggt cgttgcggtg tcggatcacc
     1081 ggtacgcgcc cgagggctcg acgcagtcga tccaggatct gcgggacagc gcccgcatcg
     1141 tcgccgacga gatcgcggca actgggtcta acgacgctag aggtcgccct tctcgcgccg
     1201 gcgcgccaga cgttcctcga cgcgacgcat gaacgactcc ttgtcggcct gcttcttggc
     1261 cgcctgagtc gcgccgcgac cgctcgcctt cgcggcgacg tgcttgcgtg gcgtgaacat
     1321 ggcccacatc gctgcggcaa gcatcaacgc aaaaccagcg atccccacgg gaatgatctg
     1381 agcgacagcg ccaatcagga ccacgccaag accgatcaac acgcccagcg caccgccgac
     1441 gatccggcca cgctggcgat agccacgagc catcgtgcga cccaatttgg ggtcagtgcc
     1501 gaggtcgcgc tccaattgat cgagcacgcg ctgctcatac tcggacagcg gcatcatgac
     1561 ctcctttgac cctggtcgtc actcatagtg taaatccgaa cggaaccgac gactagccca
     1621 ccgctcaaga aggtggcgtc ctactaacgc ctcacggccc atcagttgtt cccatcggcc
     1681 ggacgtgcga gacatgacaa agccgccgct cctttgcagg aacggcggct cgtcggaatg
     1741 tggcgaccct tagagggcgc gcacatcctc tgcctgcgga cccttggggc cctgtgtcac
     1801 ttcgaactcg acgcgctgcg cctcgttgag ggacttgaaa ccatcggtga tgatcgcgga
     1861 gtagtgaacg aagacgtcag cgcctccacc gtcctgcgcg ataaagccgt agcccttttc
     1921 cgcgttgaac cacttaacgg aaccttgtgc catgcctgta attccttgcc ttgagatgcc
     1981 cgggacggga agcggggcaa cttgccctgc tcgtcgccgc aatgccttgt cccgtgcccc
     2041 caaagaggca ggtcttgcac actacgtact tgcaaccgcc tctatcatgc cacggattag
     2101 tcgtttggtg gaagatctcg gcgttgttga gcaaggaatt tttcaaattc tgcccccagc
     2161 tcgtccgccg tgggcagcgg tccatccact tcgatgccgt cgcgacgggc gg
//
LOCUS       DASRKH010000070         4760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_32586, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000070 DASRKH010000000
VERSION     DASRKH010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32586"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1191)
                     /locus_tag="VFC06_01160"
     CDS             complement(<1..1191)
                     /locus_tag="VFC06_01160"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020013211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HZL80545.1"
                     /translation="MTWSHVDNKWPTDGLSFGADYNPEQWDRSVWREDMTLMLEAGVN
                     LVSLGIFSWGLLEVADGEYDWAWFDEIVDLLEDNGIAIDLATPTAAPPGWLLHEHPEI
                     LPVEHNGLKHSPGARLGWCPSSPIFREYSLRIVRALADRYGARDHVVMWHVSNELGGG
                     NGHCYCDVSAAAFRRWLRDKYTSLDGLNAAWNSAFWGHTYSDFEHVLPPRADASKNPS
                     LVLDFDRFSSDELLQHYLAERTVLKEVTPGLPVMTNFMVGAEQDAVDYPRWSPHMDIL
                     ANDHYTRSSDPISAQDVAFSGDRMRALTTDRRPWILMEHSTSAVNWQPRNRAKRAGEA
                     VRNSLSHVAHGADGILFFQWRASRGGAEQFHSAMVPHAGPNTAVFRAVKELGGHLRAL
                     APVQG"
     gene            complement(1386..2552)
                     /locus_tag="VFC06_01165"
     CDS             complement(1386..2552)
                     /locus_tag="VFC06_01165"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HZL80546.1"
                     /translation="MSTTPTQAAERLRLNSRWPSLHPTTQAAFNEILWKGGLPRIEIA
                     RALGVSRTRLTAITRDLDDAGLIVEGGREQRSSTGRPAEMLYARTDRYHFLGVHVRLG
                     SVIGVVVDLANRAVWERQIDLPDLDVDAVFDQACQWFVEASAEGLTVAAIAVCAPIPE
                     SGSAVRSLDVAIFRSGDGRRRVEAACGIPVWIEEDIVALTAFEQWPQLAGGQESMALI
                     SLGSEISFGLVADLKIIVGAHQSAGRFGHVPVATEGPVCPLGHRGCLWSTSSTGAIAA
                     AVPGAATVQEVASAARDGDARAAAALDTAARGLGAGAGHVVNLLDPDKLVLTGESHTV
                     LSDRLHLFHEGLRTVQITDAPVEVSVASIDFVEWARAAGAYASYRTLSGSSSQA"
     gene            complement(2622..4070)
                     /locus_tag="VFC06_01170"
     CDS             complement(2622..4070)
                     /locus_tag="VFC06_01170"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HZL80547.1"
                     /translation="MTLGAGQSEQISDELGRRLASITPSERQVAWQEMEFYAFVHFGM
                     NTSTGVEWGIGHEDPALFDPSELDVDGWMTAIASAGMTGVILTAKHHDGFCLWPSRAT
                     KHTVAASPWQSGRGDIVREVAEAAARHNLRLGMYLSPWDRTEATYGTGTAYDDFFVAQ
                     LTELLSDYGQLFSVWFDGACGEGANGKVQVYDWDRYYRVIRELQPDAVISVCGPDVRW
                     CGNEAGSTRSDEWSVVPASLLDQERIADKSQHQDDGEFARLVHSDEDDLGSRSALRGH
                     LDDLAWYPAEVNTSIRPGWFHHPEEDDKVRAAEELMGVWYSSVGGNACFLLNVPPTAA
                     GKIAAPDVDVLDQLGRLIADYSARGVSTTATVSSGVVPAGFGSAADARRAAWEPTSDD
                     AQPTVELRFDRPLCIEAIEVREDIRTGQRIEDLVVVGKNGDEAFSLGSVHAVGHRRIL
                     RFEPRILDNARVEIRGSRDVPRLLAVTAVAAR"
     gene            complement(4315..>4760)
                     /locus_tag="VFC06_01175"
     CDS             complement(4315..>4760)
                     /locus_tag="VFC06_01175"
                     /inference="COORDINATES: protein motif:HMM:NF020121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HZL80548.1"
                     /translation="AGLPVRSEVLGGFTATVWGERLRLDDGEAVATFTGDLDGSPAVL
                     RRRAASGGEAWYVGTVPPQPVLDAILGRAAASAGVVGALGPDPLPDGVEVVRRGDVLF
                     LLNAGNEERVVALPGVHRDLLTAVLFSEAVLLGPQDAVALVAETA"
BASE COUNT          804 a         1646 c         1571 g          739 t
ORIGIN      
        1 gccctggacc ggtgccaggg cgcgaaggtg gcctccgagt tccttgacgg cacggaacac
       61 agcggtattg ggacccgcgt gcggcaccat cgcggagtgg aactgctcgg ctccgccgcg
      121 cgaggcacgc cactggaaga agaggatgcc gtctgcgccg tgcgcgacgt ggctcaggct
      181 gttccgcacc gcttccccgg cccgcttggc ccggttccgg ggctgccagt tcacggcgct
      241 cgtcgagtgc tccatcaaga tccacggacg tcgatccgtc gtgagcgcgc gcatccggtc
      301 gcccgagaag gcgacgtcct gcgcagagat cgggtcggac gaccgcgtgt agtggtcgtt
      361 cgcgaggatg tccatgtggg gggaccaccg tggatagtcg acggcgtcct gctccgcccc
      421 gaccatgaag ttcgtcatga cgggaaggcc gggagtgacc tccttcagga cggtccgttc
      481 cgcgaggtag tgctggagga gctcatcgga ggagaaccgg tcgaagtcaa gcaccaggga
      541 aggattcttg gaggcgtccg cccgaggcgg caagacatgc tcgaagtcgc tgtacgtgtg
      601 accccagaag gcgctgttcc acgccgcgtt gaggccatcc aggctcgtgt acttgtcgcg
      661 cagccagcgg cggaacgctg cggccgagac gtcacagtag caatggccgt tgccgccgcc
      721 gagctcgttg ctgacgtgcc acatcaccac gtggtcgcgg gccccgtagc ggtccgcgag
      781 ggcacgaacg atccgcaggg agtactcccg gaagatcggg ctgctggggc accagccgag
      841 gcgggcgccc ggtgagtgct tcaggccgtt gtgctcgacc ggcaggatct cggggtgctc
      901 gtggagcagc cacccgggcg gcgctgcggt cggcgtcgcc agatcgatgg cgatgccgtt
      961 gtcctcgagg agatcgacga tctcgtcgaa ccacgcccag tcgtattcgc cgtcggcgac
     1021 ctcaagaagg ccccaggaga agatcccgag ggagacgagg ttgacgccgg cctcgagcat
     1081 gagcgtcatg tcttcgcgcc aaaccgaacg gtcccactgt tcgggattgt agtcagcccc
     1141 gaagctcaaa ccgtcagttg gccacttgtt gtccacgtgg gaccaggtca tccttttact
     1201 ccttcagaac aaggtcgagc cgccagatcg ctgcaggaac agaccagagc gatgaggggc
     1261 cagatcgcga tggcagcgcc gatgatcgcg agtgaatcca gggccgggga tccggaccct
     1321 cggttgagca gttcacccag catcgctctc cgcgaccggg tttgtcaatc gccaaaacaa
     1381 acaggtcaag cctgagaact agagccgctc agggttctgt acgaggcgta ggccccggcc
     1441 gcccgggccc actccacgaa gtcgatcgac gcaacgctga cctcgaccgg agcgtcggtg
     1501 atctgcaccg tgcgcagccc ttcgtggaag aggtggagcc tgtcggacag gaccgtgtgg
     1561 ctctcgccgg tcagcacgag cttgtcggga tccagcaggt tgacgacgtg tccggcgccg
     1621 gcgccgaggc cgcgggccgc ggtgtcgagc gccgcggccg cgcgagcgtc gccatccctg
     1681 gcggccgagg cgacctcctg gaccgtcgcc gcaccgggaa cggccgccgc gatcgcaccc
     1741 gtggaactgg tgctccagag gcagcctcga tgcccaagag ggcacacagg gccctcggta
     1801 gccaccggaa cgtggccgaa tcgtccagcc gactgatggg cgccgacgat gatcttcaga
     1861 tcggcgacga gcccgaagct gatctccgag ccaagcgaga tcagggccat gctctcttga
     1921 ccacccgcca gttgcggcca ctgctcgaac gccgtcagcg cgacgatgtc ctcctcgatc
     1981 cacacgggaa tcccgcaggc ggcctcgacc cggcgccgcc cgtctccgga gcggaagatg
     2041 gcgacatcaa gcgatcggac ggcgctcccg ctttccggga tcggcgcgca gacggcgatt
     2101 gccgccaccg tcaacccctc cgcgctcgct tccacgaacc actggcaagc ctgatcgaag
     2161 acggcgtcga catcgagatc gggaaggtcg atctggcgct cccagacggc ccggttggca
     2221 aggtcgacga cgacgccgat gacgcttccg agccggacgt gcactcccag aaagtgatag
     2281 cgatcggttc gggcgtagag catctcggcg ggacgaccag tactcgatcg ctgctcacga
     2341 ccgccttcga cgatgaggcc cgcgtcgtcg agatcacgcg tgatcgcggt cagcctcgtg
     2401 cgagagaccc cgagggcgcg agcgatctcg atccggggca gcccaccctt ccagaggatc
     2461 tcattgaagg cagcttgcgt cgtcggatga aggctcggcc accgggagtt caatcgcaac
     2521 cgctcggctg cctgcgtggg agtcgtcgac acctgttcat ccattcggtt tcgcctcacg
     2581 tgtggagccg gtccggtcat tgcggcgtcc gggtcgcctc gtcacctggc tgccacggca
     2641 gtgaccgcca agagtcgggg cacatcacgg ctgccccgga tctccacccg cgcgttgtcc
     2701 agaatgcgag gctcgaaccg aagaatacga cgatggccga cggcatgaac ggagcccaac
     2761 gagaaggcct cgtcaccgtt cttcccgacc acgacgagat cctcgatccg ctgcccggtc
     2821 cggatgtcct cccgcacttc gatcgcctcg atgcacaagg gccggtcgaa gcgcagttcc
     2881 accgtcggct gggcgtcgtc ggacgtgggc tcccacgccg ctctccgagc gtcggctgcc
     2941 gagccgaagc ccgccgggac gacgcccgac gataccgtcg ccgtggtcga cacgccgcgg
     3001 gcagagtagt cggcgatgag ccgtcccagc tggtccagga cgtcgacgtc tggtgcggcg
     3061 atcttccccg cggcagtcgg cggcacgttc agcaggaagc aggcattgcc gcccacggac
     3121 gagtaccaga ctcccatcag ctcctccgca gcgcggacct tgtcgtcctc ctcggggtgg
     3181 tggaaccagc cgggccggat cgacgtgttg acctcggccg gataccaggc cagatcatcg
     3241 agatgacccc gcaacgcgga ccggctaccc aggtcgtcct cgtcgctgtg gacgagccgg
     3301 gcgaactccc cgtcgtcctg gtgctgtgac ttgtcggcga tccgctcctg atcgagcaag
     3361 ctcgcaggga cgacgctcca ttcgtcgctc cgcgtcgaac ctgcctcgtt tccgcaccac
     3421 cgcacgtcgg gcccgcacac gctgatgacc gcgtccggct ggagctcgcg gatgacccgg
     3481 tagtagcgat cccagtcata gacctgaacc ttgccgttgg ctccctcacc gcaggcaccg
     3541 tcgaaccaga cggagaagag ctgcccgtag tccgacagga gttccgtcag ctgtgccacg
     3601 aagaagtcgt cgtaggcggt gcccgtgccg taggtcgcct ccgtgcgatc ccagggcgac
     3661 aggtacatgc caagacggag gttgtgccgg gcagccgcct cggcgacctc acgaacgatg
     3721 tccccccgac cggactgcca tggcgacgcc gcgacagtgt gtttcgttgc ccgactcggc
     3781 cagaggcaga agccgtcgtg gtgcttggcg gtcagaatca cgcccgtcat gcccgccgag
     3841 gcgatggcgg tcatccaccc gtccacgtcg agctccgacg ggtcgaagag agcaggatcc
     3901 tcgtggccga tcccccattc aaccccggta ctggtgttca tgccgaagtg cacgaacgcg
     3961 tagaactcca tctcctgcca cgcgacctgc cgttcgctcg gcgtgatact cgccagacga
     4021 cgccccagtt cgtcgctgat ctgttcgctc tgacctgctc ctaatgtcac ggacactccc
     4081 tactccaacg tatgtggaga ttgtattgtt ggtttacaaa ccacgtgagc ataacttgca
     4141 aaggccgccc agcgccagtc cggccttccg gaaattgcgg caggcgaggg cggggtctgc
     4201 ggcccgccgg gtgcccgtgc ttgccgccct gcgccggccg tcgggcggcc gatcccggtg
     4261 accgcgcccc tggccgctcc gcgtcgaaga acccgttccg gtaggtgacg ccgctcacgc
     4321 ggtctcggcg accagcgcga cggcgtcctg gggaccgagc agaaccgctt cgctgaacag
     4381 cactgcggtg agcagatccc ggtgcacgcc gggcagcgcc acgacgcgct cctcgttgcc
     4441 ggcattgagc aggaacagca cgtcgccgcg ccggaccacc tcgacgccgt cgggcagcgg
     4501 gtccggcccg agcgcgccga ccactccggc cgaggccgcc gcgcgaccca agatcgcgtc
     4561 gagcaccggc tgcgggggca ccgtccccac gtaccaggcc tcgccgccgg acgctgcgcg
     4621 ccgccggagc accgcgggcg agccgtcgag gtccccggtg aacgtcgcga cggcctcgcc
     4681 gtcgtccagg cgcagccgtt cgccccacac ggtcgcggtg aagccgccga gcacctcgga
     4741 gcgcaccggc agcccagcgt
//
LOCUS       DASRKH010000071         2276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_55308, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000071 DASRKH010000000
VERSION     DASRKH010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55308"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1453
                     /gene="ilvB"
                     /locus_tag="VFC06_01180"
     CDS             <1..1453
                     /gene="ilvB"
                     /locus_tag="VFC06_01180"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HZL80549.1"
                     /translation="VAASHIGTDAFQEADIVGITAPITKHNMLIKDPVDIGPAIAQAF
                     YIASHGRPGPVLVDIAKSALQSSAPFVWPDRVELPGFNAGAKPHAKSIQEAARLLATA
                     RRPVLYVGGGVIRAGASEGLRKLVDLSGAAVVTTLMARGALPDSHPQHLGMPGMHGTV
                     PAVASLQKADLVVSLGARFDDRVTGKLDSFAPHATVVHADIDPAEIGKNRHADVPIVG
                     DLKDVLQQLVPALHEEHRKHGKPDLEAWWLQINDWRVTYPLGYAATQDGLSSPQHVIQ
                     RLGALNDPQSTIFTSGVGQHQMWASQFIKYERPNAWINSGGLGTMGFSVPAAMGAKVG
                     QPDQTVWAIDGDGCFQMTNQELTTCALNEIPIKVGIINNSSLGMVRQWQTLFYNARYS
                     NTDLHTGHGTRRVPDFVKLAESMGCVGLRCESDADVDATIKRANEINDQPVVVDFTVS
                     KDAMVWPMVNAGVSNDEIQYARGVAPEWDREEA"
     gene            1456..1977
                     /gene="ilvN"
                     /locus_tag="VFC06_01185"
     CDS             1456..1977
                     /gene="ilvN"
                     /locus_tag="VFC06_01185"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HZL80550.1"
                     /translation="MSNKHTLSVLVENKPGVLARVAGLFSRRAFNIHSLAVGPTEHPE
                     ISRITVVVDVDDLPLEQVTKQLNKLVHVLKIVELDNAKSVRRELLLVKVRCDARQRAD
                     VLQVVELFRAHVVDVAPDSLVIEAIGNPDKLEALLAALSPHDLREIVQSGTVAIGRGA
                     RSITERALDRASA"
     gene            2049..>2276
                     /locus_tag="VFC06_01190"
     CDS             2049..>2276
                     /locus_tag="VFC06_01190"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801492.1"
                     /note="catalyzes the formation of
                     (R)-2,3-dihydroxy-3-methylbutanoate from
                     (S)-2-hydroxy-2-methyl-3-oxobutanoate in valine and
                     isoleucine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HZL80551.1"
                     /translation="MAELFYEKDADLSIVQGMKVAIVGYGSQGHAHAQNLRDSGVEVK
                     VALRDGSRSAIKAENDGFTVLPVSDAAEWADL"
BASE COUNT          405 a          780 c          736 g          355 t
ORIGIN      
        1 ggtggccgcg tcgcacatcg gcacggacgc gttccaagag gccgacatcg tggggatcac
       61 ggcgcccatc accaagcaca acatgctgat caaggacccg gtcgacatcg ggccagcgat
      121 tgcacaggcc ttctacatcg cgagtcacgg ccgccccggc ccggtgctgg tggacatcgc
      181 caagtccgcg ctgcagtcga gcgcgccgtt cgtctggccc gaccgcgtcg aactgcccgg
      241 cttcaatgcg ggcgccaagc cgcacgccaa gtccatccag gaggccgccc gcctgctcgc
      301 caccgcccgt aggcccgtgt tgtacgtcgg tggtggcgtc attcgcgcgg gggcatcgga
      361 ggggctgcgc aaactggtcg acctgtccgg cgcggccgtc gtgaccaccc tgatggcgcg
      421 aggggcattg cccgatagcc acccgcagca cctcggcatg ccggggatgc acggcacggt
      481 tcccgccgtg gcctccctgc agaaggccga cctcgtggtg tcgctgggcg cgcgcttcga
      541 tgaccgcgtc accggcaagc tcgacagctt cgccccacac gccaccgtcg tgcacgctga
      601 catcgacccc gccgagattg gcaagaaccg tcacgcagac gtgccgatcg tcggcgacct
      661 caaggacgtg ctgcagcagc tggtgccggc gctccacgag gagcaccgca agcacggcaa
      721 gcccgacctc gaggcctggt ggctgcagat caacgactgg cgcgtcacgt acccgctcgg
      781 ctacgccgcc acgcaggatg gcctgtcaag cccgcagcac gtgatccaac gcctgggcgc
      841 gctcaacgac ccgcagtcaa ccatcttcac gtcgggcgtg ggccagcacc agatgtgggc
      901 cagccagttc atcaagtacg agcgccccaa cgcgtggatc aactccggcg gccttggcac
      961 gatgggcttc tcggtccccg ccgcgatggg cgccaaggtc ggccagcccg accagacggt
     1021 gtgggccatc gacggcgacg gctgcttcca gatgaccaat caggagctca ccacctgcgc
     1081 gctcaacgag atccccatca aggtcgggat catcaacaac agttcgcttg gcatggtgcg
     1141 ccaatggcag acgctgttct acaacgcccg ctactcgaac accgacctgc acaccggcca
     1201 cggcacccgc cgcgtgcccg actttgtgaa gctcgccgag tcgatgggtt gcgtgggctt
     1261 gcgatgcgag tccgatgccg acgtggacgc gaccatcaag cgcgccaacg agattaacga
     1321 ccagccggtg gtggtggact tcaccgtgtc caaggacgcg atggtgtggc ccatggtcaa
     1381 cgccggcgtg agcaacgatg agattcagta cgcgcggggc gttgcccccg agtgggaccg
     1441 cgaggaggcg tgaccgtgag caacaagcac actttgtccg tactggtcga gaacaagccc
     1501 ggcgtgctcg cacgcgtggc cggattgttc tcgcgacgcg ccttcaacat ccattcgctg
     1561 gcggtgggcc ccaccgagca cccggagatc tcgcgcatca cggtggtggt cgacgtggat
     1621 gacttgccgc tcgagcaggt caccaagcag ctcaacaagt tggtgcatgt cctcaagatc
     1681 gtggagctcg acaatgcgaa gtcggtgcgg cgcgagctgc tgctggtgaa ggtgcgttgc
     1741 gacgcgcgcc agcgtgccga cgtgctccag gtcgtcgagc tcttccgcgc ccacgtcgtg
     1801 gacgtcgcgc cggactcgct cgtcatcgag gcgatcggca accccgacaa gctcgaggca
     1861 ttgcttgcgg ccctgtcgcc gcacgacctg cgcgagatcg tccagtccgg caccgtggct
     1921 atcggccgtg gcgctcgctc catcacggag agggcgcttg accgcgccag cgcctgacaa
     1981 gcactagttt ctagaaagta gcccggttca ctcgcgggca tcccaaccca aggagaagac
     2041 acacgactat ggctgagttg ttctacgaga aggacgccga cctctcgatc gtccagggaa
     2101 tgaaggttgc gatcgtcggt tacggctcgc agggccacgc ccacgcgcag aacctgcgcg
     2161 actccggcgt tgaggtgaag gtcgcgctgc gcgacggctc gcgttcggcc atcaaggcgg
     2221 agaacgacgg cttcaccgtg ctgcccgtgt ccgatgccgc agagtgggcc gacctc
//
LOCUS       DASRKH010000072         2680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_10059, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000072 DASRKH010000000
VERSION     DASRKH010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10059"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VFC06_01195"
     CDS             <1..310
                     /locus_tag="VFC06_01195"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZL80552.1"
                     /translation="LDGAGHLRTFASIVMPNVRPAMATFGIFAFMSSWNAFLWPLVIL
                     RSDELKTLPLALASLQGQFTTQWDVMMAGSVVSIIPMLALYIFAQKYIIQGVAGSGIK
                     "
     gene            345..1616
                     /locus_tag="VFC06_01200"
     CDS             345..1616
                     /locus_tag="VFC06_01200"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HZL80553.1"
                     /translation="MHKSAIAGVGIVAALALTLTACSSSDEPTASSGGATSSAAEPVT
                     ITYSNFISNGGNEDNLAAIVSAFEADNPTITVDVTTLPYADYFTALQTDLAAGTTADV
                     FDIEYATYPTYQSAGAFAPLEGVDSSVYRGDVAAAYQTDGVAYALPTSFSTVVLYFNK
                     DLFDAAGLDYPTSDWTWADEKAAAEALTDTGAGVWGDYQPISYYEFYKALVQAGGQFL
                     SDDGTSVAFDSPEGIAAAQWLVGKSGSTMPTAEQGAGTPDFDTSLWADGKLAMWHSGI
                     WMFGAAADSSFAWDIAVEPGDAANASAVFSNAVAVSANSSHKEAAQMFAEYLTSSQVM
                     VDTRLSAGWELPAISDEALLATYLDQGAPDNRQAVFDSLDGIALPPVIGDNQAEMQDI
                     IGALLTEAAAGRMSVEDALASAASQVNDLLG"
     gene            1705..2619
                     /locus_tag="VFC06_01205"
     CDS             1705..2619
                     /locus_tag="VFC06_01205"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HZL80554.1"
                     /translation="MTPVTSVPARALLDSSRDLILSLQTPEGAYPASPTFSAYKGFCW
                     FRDGAFIADGMSAAGEVASVDAFFAWCARVVLRYRDDIRAAVDAATAGEPLPDERMLP
                     ARFTFDGARAGGDDWWDFQLDGFGTWLWALDAHASRHGTDLSPYVEAVAITVDYLVSS
                     WNRPCFDWWEEHDDQIHISTLGCIGAGLEAASRMGVLDLARVGLAASTAATIRHFVLT
                     DGVVDGHLVKWMGSDAVDGSLTSLIAPLRFIPADHPVARATVEAVEADLAVDAAVHRE
                     VGLDGFDGGPRYRVIGRDEAEGRDQRRE"
BASE COUNT          442 a          960 c          810 g          468 t
ORIGIN      
        1 gctcgacggc gcgggccacc tgcgcacctt cgccagcatc gtcatgccca acgtccgtcc
       61 cgccatggcc acgtttggca tcttcgcctt catgagtagt tggaacgcgt tcctgtggcc
      121 gctcgtcatc ctgcgttccg acgaactcaa gactttgccg ctcgccctgg cgtccctcca
      181 agggcaattc accacccaat gggacgtcat gatggctggc tccgtggtga gcatcattcc
      241 gatgctcgcc ctctacatct tcgcccagaa gtacatcatt cagggcgtag caggctcggg
      301 aatcaagtaa cccgggcacc acccagaaac cgaaggaata tcccatgcac aagtcagcaa
      361 tcgccggggt cggcatcgtt gccgccctcg cactgacgct cactgcgtgc tcgtcaagcg
      421 acgagcccac agcgtcatcg gggggcgcta cgtccagcgc agccgagccg gtcaccatca
      481 cctactcgaa cttcatctcg aatggcggga atgaagacaa cctggccgcc atcgtctctg
      541 catttgaggc ggacaacccc accatcacgg tggatgtcac gacgctgccg tacgccgact
      601 acttcacagc cctccagacg gacctggccg caggtactac ggcggacgtg ttcgacatcg
      661 agtacgccac ctacccgacg taccagtcgg ccggagcgtt cgcacccctc gagggcgtcg
      721 actcctccgt gtatcgcggc gacgtcgccg cggcctacca gaccgacggc gtggcctacg
      781 cgctaccgac gtcattctcg accgtggtcc tgtacttcaa caaggatctc ttcgacgccg
      841 ccgggctcga ctacccgacg tccgactgga catgggccga cgagaaggcc gcggccgagg
      901 cgctcaccga cactggcgcc ggcgtgtggg gcgactacca gcccatctcc tactacgagt
      961 tctacaaggc actcgtgcag gcgggcggcc agttcctcag cgacgacggc acctccgtcg
     1021 ccttcgactc gcctgaaggc attgccgccg cgcagtggct cgtgggcaag tcgggatcga
     1081 cgatgccgac ggcagagcag ggcgctggca ccccggactt cgacacgagc ctgtgggcgg
     1141 atggcaagct cgccatgtgg cactcgggca tctggatgtt cggtgcagcc gctgacagta
     1201 gcttcgcgtg ggacatcgcc gtcgaaccgg gcgacgccgc caacgcatcg gccgtgttct
     1261 ccaacgccgt ggccgtgtct gcgaactcgt ctcacaagga ggcggcacag atgttcgccg
     1321 agtacctcac cagctctcag gtgatggtgg acacgcgtct gagtgcaggt tgggaactac
     1381 cggccatctc ggacgaggcg ttgctcgcga cgtacctcga ccagggcgct cctgacaacc
     1441 gtcaggccgt gttcgactcg ctcgacggca tcgccttgcc gcccgtcatc ggcgacaacc
     1501 aagccgagat gcaggacatc atcggcgctc tactgactga ggccgctgcg ggtcgcatgt
     1561 cggtagagga cgcacttgcg tccgctgcct cgcaggtcaa cgacctgctc ggctgacgca
     1621 tgacgagggc ctggtggcgg cgcccagaat tcgccgccac caggccttgc gagcctaccc
     1681 cgacgtccag aaacgagtgc cctcatgacc ccggtcacat ccgtgcccgc tcgcgccctt
     1741 ctcgatagct cccgcgacct catcctctcc cttcagaccc ccgaaggcgc ttaccctgcc
     1801 agccccacgt tctcggcgta caagggcttc tgctggttcc gcgacggcgc attcatcgcc
     1861 gacggaatgt ctgcggcggg tgaagtggcc tcggtagacg ccttcttcgc ctggtgtgca
     1921 cgcgtcgtcc tccggtaccg cgacgacatt cgcgcggccg tcgatgccgc aaccgcgggc
     1981 gaaccccttc ccgacgaacg catgctgccc gcccgcttca ccttcgacgg cgcgcgcgct
     2041 ggcggcgacg actggtggga cttccaactg gacggctttg gcacctggtt gtgggctctc
     2101 gacgcccacg cctcgcgcca tggcaccgac ctctcgccct acgtcgaggc tgtcgcgatc
     2161 accgtcgact acctggtctc atcctggaat cgcccgtgct tcgattggtg ggaggagcac
     2221 gacgatcaga tccacatctc gaccctcggg tgcatcggag ccggactcga ggccgcctcc
     2281 cggatgggag tgctcgatct tgcccgcgtc ggtctcgcgg cctccaccgc ggccaccatt
     2341 cgtcacttcg tcctgacaga cggcgtcgtc gacggccacc tcgtcaagtg gatgggttcc
     2401 gacgccgtgg acggctcact cacgtcgctg atcgcgcccc tccgcttcat ccctgccgat
     2461 cacccggtag cgcgggccac ggtcgaagcc gtcgaggccg acctcgcggt ggacgccgcc
     2521 gtccaccgcg aggtcggcct cgacggcttc gacggtggcc cgcgctaccg ggtgatcggc
     2581 agggatgaag cggaggggcg cgatcagcga cgtgagtgag ccgtccacgg cgtcggaacc
     2641 catccacttg acgaggtggc cgtcgacgac gccgtctgtc
//
LOCUS       DASRKH010000073         3232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_51225, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000073 DASRKH010000000
VERSION     DASRKH010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51225"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VFC06_01210"
     CDS             complement(<1..258)
                     /locus_tag="VFC06_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZL80555.1"
                     /translation="MMPRPDAVVRPDGSPVHVLVVDDEEVLAELLSSALRYEGWNVVS
                     APDGYGAIRQVKAHDPDVIVLDMMLPDLDGLEVLRRARQLKP"
     gene            459..3113
                     /gene="topA"
                     /locus_tag="VFC06_01215"
     CDS             459..3113
                     /gene="topA"
                     /locus_tag="VFC06_01215"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HZL80556.1"
                     /translation="MARKLVIVESPAKARTISAFLGKGFDVEASIGHIRDLPQPSDLP
                     PEMKKGPYAKFAVDVEHGFDPYYVVYPDKKKKVTELKRLLKDADELYLATDEDREGEA
                     IAWHLLEVLKPKVPVKRMVFHEITAEAIQRAIGQTRDVDEHLVDAQETRRILDRLVGY
                     EVSPLLWRKIGPGLSAGRVQSVATRLVVERERERIAFRSAGYWDVTGRFARGEEGFEA
                     RLSSVDATRVATGRDFGDDGALKSEAVHLDEATARALAAVVDRGTVRAVESKPYRRSP
                     AAPFTTSTLQQEASRKLRLSSRETMRVAQALYENGYITYMRTDSSALSKQAIAAARAQ
                     ATELYGAETVPAKPRHYGKVAKGAQEAHEAIRPAGEHFRRPSELAHLPALQRKVYDLV
                     WKRTIASQMADATGETATVRVAATAEVPAAGLRLAPGSHDLEFTASGTVITFRGFLAA
                     YEEGHDAARYEADAAESRLPALTQGEALVATDVTAAGHETTPPPRYTEASLVKAMEER
                     GIGRPSTYAATMSTIIDRGYVGKKGQALVPSWLAFSVTSLLEQNLTRLVDYDFTAAME
                     ADLDRIAAGSQDRREWLEHFYFGTGESEGLKALVEGLGDIDARAVNSRDLGEGITLRV
                     GRYGPYLETAGPDGEVRRASIPDDVAPDELTLAKARELLETRSDEGRELGTDPVSGNP
                     VVAKNGRFGPYVTEVLPEDAPAKAKPRTASLFKSMQLETIHLEDALRLLSLPRVVCTD
                     ATGAEVTAQNGRYGPYLKKGTDSRSLETEDQLFDVTMEQAEEIFAQPKQRRGAARATA
                     PLKELGADPVSGGSVVVKDGRFGPYVTDGTTNATLRKGDEIEALTAERAFELLAEKRA
                     KGPAPKRAPARKPATRKPAARTAAPKQG"
BASE COUNT          508 a         1159 c         1153 g          412 t
ORIGIN      
        1 gggcttcagc tggcgggcgc ggcgcagcac ctcgagcccg tcgaggtccg ggagcatcat
       61 gtcgaggacg atcacgtcgg ggtcatgcgc cttcacctgg cggatcgcgc cgtaaccgtc
      121 cggggcggag accacgttcc agccctcgta ccgcagggcg gaggagagca gttcggcgag
      181 cacctcctcg tcgtccacca cgaggacgtg gacaggggat ccgtcggggc gtacgacggc
      241 gtcgggcctg ggcatcatga gcccatcctc acccaccgcc ctcggccgtc gctaggagtt
      301 cgctgtgagt cggctgtgag gtcgggggac gaggtgcggc acacggcggc ggggggacgg
      361 cgtgtgcacg cccgtcggaa catgtgccag tattcccctc gggggctgcc gggacggcgc
      421 accgccgttc gtggcccccg ctcaccagga aggtcgccgt ggcacgcaag ctcgtcatcg
      481 ttgagtcgcc cgcgaaggcg cgcaccatca gtgcgttcct cgggaagggc ttcgacgtcg
      541 aggcatcgat cggccacatc cgtgacctgc cccagccgtc ggacctcccg cccgagatga
      601 agaaggggcc gtacgccaag ttcgccgtgg acgtcgagca cggcttcgac ccctactacg
      661 tggtctaccc ggacaagaag aagaaggtca ccgagctcaa gcgcctgctg aaggacgcgg
      721 acgagctcta cctcgccacg gacgaggacc gcgaggggga ggccatcgcc tggcacctcc
      781 tcgaggtgct gaagccgaag gtgccggtca agcgcatggt cttccacgag atcaccgcgg
      841 aggccatcca gcgtgcgatc ggccagaccc gcgacgtcga cgagcacctc gtggacgccc
      901 aggagacccg ccgcatcctc gaccggctgg tcgggtacga ggtgtcaccg ctgctgtggc
      961 gcaagatcgg accgggcctg tccgcgggcc gcgtccagtc ggtggccacc cgtctggtgg
     1021 tggagcggga gcgggagcgc atcgcgttcc gctccgccgg ctactgggac gtcaccggcc
     1081 gtttcgcgcg cggcgaggag ggcttcgagg cgcgcctgtc gtcggtggac gccacccgtg
     1141 tggccacggg ccgcgacttc ggcgacgacg gcgcactcaa gtccgaggct gtccacctgg
     1201 acgaggcgac ggcgcgggcg ctcgccgccg tcgtcgaccg cgggacggta cgggcggtcg
     1261 agtcgaagcc gtaccgccgc agccccgccg ctccgttcac cacctcgacg ctgcagcagg
     1321 aggcctcccg caagctgcgg ctgtcctcgc gcgagacgat gcgggtggcc caggcgctgt
     1381 acgagaacgg gtacatcacc tacatgcgca cggactcctc ggcgctctcc aagcaggcga
     1441 tcgccgccgc ccgcgcccag gccacggaac tgtacggcgc ggagacggtg cccgcgaagc
     1501 cgcggcacta cggcaaggtc gccaagggcg cacaggaggc gcacgaggcg atccggcccg
     1561 ccggcgagca cttccggcgc ccctccgagc tcgcccacct cccggcgctg cagcgcaagg
     1621 tctacgacct ggtgtggaag cgcaccatcg cctcgcagat ggccgacgcc accggcgaga
     1681 ccgccaccgt gcgcgtcgcg gccaccgcgg aggtcccggc cgccgggctg cggctcgcgc
     1741 ccgggtccca cgacctcgag ttcacggcgt cgggcacggt gatcaccttc cggggcttcc
     1801 tcgccgccta cgaggagggc cacgacgccg cgcgctacga ggccgacgcc gccgagtccc
     1861 gcctccccgc cctcacccag ggcgaggcgc tggtcgccac ggacgtcacc gcggccggtc
     1921 acgagaccac cccgccgccg cgctacacgg aggcctcgct ggtcaaggcc atggaggagc
     1981 gcgggatcgg gcggccctcc acgtacgccg ccacgatgtc cacgatcatc gaccgggggt
     2041 atgtgggcaa gaagggccag gcgctcgtcc ccagctggct cgccttctcc gtcaccagcc
     2101 tgctcgagca gaacctcacg cgcctcgtgg actatgactt cacagctgcg atggaggcgg
     2161 acctcgaccg gatcgcggcg ggctcgcagg accgccggga gtggctggag cacttctact
     2221 tcggcaccgg cgaatccgag gggctcaagg cgctggtgga ggggctcggc gacatcgacg
     2281 cccgcgccgt gaactccagg gacctgggcg aggggatcac gctgcgcgtg ggccgctatg
     2341 ggccctacct cgagaccgcc ggcccggacg gcgaggtgcg gcgcgcctcg atccccgacg
     2401 acgtcgcccc ggacgagctg accctcgcaa aggcccgtga actgctcgag acgcgcagcg
     2461 acgagggccg tgagctgggc acggacccgg tgtccggcaa ccccgtcgtg gcgaagaacg
     2521 gccgtttcgg gccgtacgtg accgaggtgc tccccgagga cgccccggcg aaggccaagc
     2581 cccggacggc gtcgctcttc aagtccatgc agctcgagac gatccacctg gaggacgccc
     2641 tgcggctcct ttcgctgccc cgcgtggttt gtaccgacgc gacgggcgcc gaggtcacgg
     2701 cacagaacgg gcggtacggt ccctacctga agaaggggac ggactcgcgc tcgctggaga
     2761 ccgaggacca gctcttcgac gtcaccatgg agcaggcgga ggagatcttc gcccaaccca
     2821 agcagcggcg cggcgcggcg cgcgccaccg ccccactgaa ggagctgggg gcggacccgg
     2881 tgagcggcgg gagcgtcgtc gtcaaggacg ggcgtttcgg cccgtacgtc accgacggca
     2941 ccacgaacgc cacgctgcgc aagggtgacg agatcgaggc actcacggcc gagcgtgcct
     3001 tcgagctgct ggcggagaag cgcgcgaagg gtcccgcccc caagcgggcg ccggcgcgca
     3061 agcccgccac gcgcaagccg gcggcacgaa ctgccgcacc gaagcagggc tgagccgcct
     3121 gaaccgtggt gcgaccgggt cgccgtggtg ccatgattcc acagtgaccg ttgcatcgac
     3181 ccttgccgtc aatgcgcgtg cggacgggtt ccgcgcgcgc ctccagatgg tg
//
LOCUS       DASRKH010000074         4487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_14432, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000074 DASRKH010000000
VERSION     DASRKH010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 4487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4487
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14432"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..1174)
                     /locus_tag="VFC06_01220"
     CDS             complement(149..1174)
                     /locus_tag="VFC06_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HZL80557.1"
                     /translation="MKTPPAALCPSIPDSVSTRLLSLLEREKIADLRRGGLSMRAIGR
                     ELGRSASTVMRELEAHTVNGRYLPHGAHRDAAAARARPKVVKLAQPGRLRDYVSQQLA
                     QQWSPEQIRHRIREDFPDDEDMRVSTETIYQALYVQARGGLKREVADALRTGRTRRKP
                     LRSTQHRTSRFVDPMVMISDRPAQIEDRAVPGHWEGDLIVGERNQSAIITLVERTTRY
                     VMLGYLPGDHSAEAVRDVLVTLIGTLPSHLRGSLTWDQGAEMAAHKAVKIATGVPVYF
                     CDPASPWQRGSNENTNGLLRQYFPKGTDLSVYGLEDLEHVAQKLNSRPRKTLGWKTPA
                     ERLLQLV"
     gene            complement(1396..2388)
                     /locus_tag="VFC06_01225"
     CDS             complement(1396..2388)
                     /locus_tag="VFC06_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007270300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZL80558.1"
                     /translation="MSDLAPLLQSFFTDKLDRHMNASVHTKAAYADAFRLLLLYAHKA
                     TGITPSELMLADLNADLIGGFLQHLETDRGNCAATRNARRAALRSFFTYASYRAPDSI
                     ATISQVLAIPAKRTKRTLVSFLTGPEAEALIGAPDTSTWIGRRDRLLLHLGIQTGLRV
                     SELVGLSIDSIQIGPHSQLECVGKGRKQRVIPLQKNTARLLAAWLRELPVVTGTPLFP
                     THAGTAMTRAAVTKLVARHVQSAAQQCPSLSGKNVTPHTLRHTCAMTLLHAGIDTASI
                     ALWLGHANIQTTQAYLHADLELKRRTLDRLPAIDDRPPARYQASDALLAFLANR"
     gene            complement(2381..3310)
                     /locus_tag="VFC06_01230"
     CDS             complement(2381..3310)
                     /locus_tag="VFC06_01230"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZL80559.1"
                     /translation="MSELRDLLDSYLTTRRALGFKLAAPGKTLNAFVSWMDERGEPTI
                     RRDLATAWVSQFTRRTVSDRLNHIRQFAEHVAWFDPATEVPLVDGNPYGSHRARPLIL
                     APEQIDALLAAAGRLTPTVRAASWQTLLGLLVVTGLRISEARNLNDADVATDESGHGG
                     WARVTDTKFGKSRLVPLHPSTMSAIRRFQRLRDQTFPVPRTAAVFVARRGTRIARSTA
                     GQTFQEIRTMAGLTGGPTSPAVRLHDLRHSFATNTLIGHIRAGGDVDAMMPVLSAWLG
                     HVSPESTYWYLSNTPELAAALAERLHEGGDDHE"
     gene            complement(3307..4245)
                     /locus_tag="VFC06_01235"
     CDS             complement(3307..4245)
                     /locus_tag="VFC06_01235"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZL80560.1"
                     /translation="MVIHCDQRREDVREVLDGFEAWLLRERSTRARTAAAYTARVAGF
                     VEWLPAPLDRSLRALTAATVIEWVNLEAARGLKASTLGKQMVMLRSFLQFCHRSAWTG
                     QDFAGVVPHAAAWRLSSIPDPVPAGMIEALFDSLDLRSGKGLRDRAILLLLTGLGLRA
                     CEIAALRLDDIGWQTGSLRICGKGDRVDELPLPDEVGQALEDYVLHGRGGRVEGDAVF
                     WTVIHPVQPLTANGVCGTVRQICIKAGVEKFGPHRLRQTFATGMLASGATLQEVQGLL
                     RHAHLSTTAIYTKVDKNRLTQFVTEWPTAASGRWGR"
BASE COUNT          768 a         1461 c         1453 g          805 t
ORIGIN      
        1 atcaaggccg ccatctcggc cacgaacggc cccggcattc cgctggatgc cactgccaag
       61 cccgaggcat agcgccccgc gcacagcgcg atacgaaggc ccggttccca ctgggcggat
      121 tcaaggggtc gtcgcaacac cgattgagtt agacaagttg tagcagacgc tcggctggag
      181 ttttccagcc gagcgttttg cgtgggcggc tgttgagttt ttgggcgacg tgttcgaggt
      241 cttcgagtcc atagaccgat aggtcagtgc ctttggggaa atactgtcgt agtagtccgt
      301 tggtgttctc gttggaccct cgttgccaag gcgacgcagg gtcgcaaaaa tagacgggga
      361 ctccggtcgc gattttgact gctttgtggg cagccatctc cgcgccttgg tcccaggtca
      421 acgacccgcg caggtggctg ggcagcgtgc cgatcagggt gacgaggacg tcgcggacag
      481 cctcggcgct gtggtcgccg ggcagatatc caagcatcac gtaacgtgtg gtgcgttcga
      541 cgagcgtgat gatcgcggac tggtttcgct cgccgacgat caagtcacct tcccaatgcc
      601 caggaacggc acggtcttca atctgcgcgg gacggtcgga gatcatgacc atcggatcga
      661 cgaaccggct cgtgcggtgt tgcgtcgagc gcaggggttt gcgacgggtc cgcccggtgc
      721 gcagggcgtc tgcgacctca cgtttgagac cgccgcgagc ttggacgtag agggcctggt
      781 agatcgtctc cgtgctcacc cgcatgtcct catcgtctgg gaaatcttcg cggatccggt
      841 gacgaatctg ctcgggagac cattgttgcg cgagctgctg cgaaacgtag tcgcgaagcc
      901 ggcctggctg ggccaacttc accactttcg gacgcgcccg tgcagctgcc gcgtctcggt
      961 gagcaccatg aggcagatac cggccgttga ccgtgtgtgc ctcgagctcg cgcatgaccg
     1021 tcgacgccga cctacccagc tcccgtccga tcgcccgcat tgacaggccg ccccgtctca
     1081 ggtccgcgat cttctctcgc tccagcaatg acaacagacg cgtcgagacc gaatctggaa
     1141 tcgacgggca cagcgcggca ggtggagtct tcatggactc caccgtcgtc ttcccggttg
     1201 cggagttcac gcgacgccca tcgggataaa accggctctt cgcagttagg gatgtaggag
     1261 tggtctgaag cctccggatt cgagcaggga tcgggcgatg atatgtcgcc ctcgtggtta
     1321 tgttgaccgg ctgtgtgagt gctctggcct cgcatgggtt ggctgctgag tcggtcaaca
     1381 taaccatcgc tggtgtcacc ggtttgcgag gaacgcgagc agagcatcgg atgcttggta
     1441 gcgggctggt ggccggtcat cgatcgcggg caggcggtcg agcgttcggc gtttgagttc
     1501 caggtcggcg tggagatagg cctgcgtggt ctggatgttc gcatggccga gccagagcgc
     1561 gatgctcgcg gtgtcgatcc cggcgtgcag gagcgtcatc gcgcaggtgt gccgcagcgt
     1621 gtgcggggtg acgttcttcc cgctcagaga aggacattgc tgggccgcgg actggacgtg
     1681 tcgggcgacg agtttcgtga ccgccgccct ggtcattgcg gtcccggcgt gcgtcgggaa
     1741 cagcggcgtg cctgtgacga cggggagctc gcggagccaa gcagcgagga gtcgtgcagt
     1801 gttcttctgc aacgggatga cgcgttgctt gcgtcccttc ccgacgcatt cgagttggct
     1861 gtgcgggccg atctgaatgc tgtcgatgct cagcccgacg agttcgctga cccgcagtcc
     1921 ggtctggata cccaggtgta gtaggagtcg gtcacgcctg ccgatccagg tgctcgtgtc
     1981 cggggcaccg ataagtgctt cagcttcggg gccggtgagg aacgatacga gcgttctctt
     2041 cgtgcgcttc gcggggatcg cgaggacctg gctgatcgtg gcgatcgagt cgggggcccg
     2101 gtaactggcg taggtgaaga atgaccgcaa ggccgctcgg cgggcgttcc tcgtggctgc
     2161 gcagttcccg cgatcggttt ccagatgctg gagaaacccg ccgatcaggt ccgcgttcag
     2221 gtcggccagc atcagctcgg atggtgtgat gccggtcgct ttgtgggcgt agagcagcag
     2281 cagacggaag gcgtcggcgt aggcggcctt cgtgtggacg ctggcgttca tatgccggtc
     2341 gagcttgtcg gtgaagaagc tttgcagcag cggcgcgagg tcactcatga tcgtctccgc
     2401 cctcgtgcag ccgctcggcg agggccgcgg ccagttcggg agtgttcgac agataccagt
     2461 aagtcgactc cgggctgacg tggccgagcc aggctgacag caccggcatc atcgcgtcaa
     2521 cgtcaccgcc agcacggatg tgtccgatga gcgtattcgt cgcgaacgag tgcctaaggt
     2581 cgtgcagcct gaccgccggc gaggtcgggc cgcctgtcag tcccgccatc gtgcggatct
     2641 cctggaacgt ctggcccgcc gtggaccggg cgatcctggt gccgcgacgc gcgacgaaca
     2701 ccgccgccgt cctcgggacc gggaacgtct gatcacgcaa ccgctggaac cggcgaatcg
     2761 cactcatcgt ggacggatgc agtggaacga gtcgggactt gccgaacttc gtatccgtca
     2821 cccgcgccca gccgccgtga ccggactcgt ccgtggcgac gtcggcatcg ttcaggtttc
     2881 gggcctcgct gatccgtagc cccgtcacca ccagcaaccc aagcaaggtc tgccacgacg
     2941 ccgcacggac cgttggtgtc agcctgcccg ccgcagccag gagtgcgtcg atctgctccg
     3001 gtgcgaggat cagtggacgg gcgcggtgac tgccataggg gttcccgtcg accagcggga
     3061 cttcggtggc aggatcgaac caggcgacgt gctcggcgaa ctgccgaatg tggttcaacc
     3121 ggtccgacac cgtccggcga gtgaactgcg agacccatgc cgtggccagg tcacggcgga
     3181 tcgttggctc gccgcgctcg tccatccagg acacgaacgc gttcagcgtc ttgcccggcg
     3241 cggccagttt gaacccaagc gcgcggcgtg tggtcaggta actgtccagg aggtcacgca
     3301 actcgctcac cgtccccacc gccccgacgc cgcagtcggc cactcggtca cgaactgggt
     3361 cagccggttc ttgtcgacct tcgtgtagat cgcggtcgtg ctcaggtgcg cgtgccgcag
     3421 caggccctgg acctcctgca gggtggcgcc ggaggcgagc atcccggtcg cgaacgtctg
     3481 cctgagccga tgcgggccga acttctccac cccggccttg atgcagatct gccggacggt
     3541 cccgcagacc ccgttcgccg tcaacggctg gaccggatgg atcaccgtcc agaacaccgc
     3601 gtcgccctcg acccggccgc cgcgcccatg aaggacatag tcttccaacg cctgcccgac
     3661 ctcgtcaggc aaaggcaact cgtcgacacg gtcgcccttg ccacagatac gcaaactccc
     3721 cgtctgccac ccgatgtcat cgagtctgag cgcggcgatc tcgcacgccc gcagaccgag
     3781 cccggttaac agcagcagaa tcgctcggtc ccgcagccct ttccccgagc gcaagtccag
     3841 actgtcgaac agggcctcga tcatgccagc agggaccggg tccgggatcg aggacagccg
     3901 ccatgctgct gcatgcggca ccaccccggc gaagtcctgc cccgtccagg ccgagcggtg
     3961 gcagaactgc aggaacgaac gcagcatcac catctgcttg cccaaagtcg acgccttcag
     4021 cccgcgcgcg gcttccaaat tcacccactc gatcaccgtc gcggccgtca acgcccgcaa
     4081 cgaccgatca agcggagcag gcagccactc cacgaaccct gcaacgcgag cggtatacgc
     4141 cgccgcggtg cgcgcccgcg tcgacctctc ccgcaacagc catgcctcga acccgtccag
     4201 cacctcgcgc acatcctcgc ggcgctgatc acaatgaatc accatctcgg tcctcctcga
     4261 cccgacgcca ggcgatccca acaaccaccc agtcagatca agcgaacccc gcttcggccg
     4321 ccgatggtta tgttgaccgg atcagccgcc cagccccgca cgaccaggga cgccaccaga
     4381 ccggtcaaca taaccacgag ggcgacatac catcgcccgc tccctgctcg aatccggcgg
     4441 cttcagaccg ctgctacacc ctcaattgtg aagagccact aattgtc
//
LOCUS       DASRKH010000075         2234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_13256, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000075 DASRKH010000000
VERSION     DASRKH010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13256"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="VFC06_01240"
     CDS             complement(<1..986)
                     /locus_tag="VFC06_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZL80561.1"
                     /translation="MGTAKLVDVARRAGVSVGTVSNVLNRPDQVRPATREKVEQAVRE
                     LGFVRNDSARQLRSGTSRLLAYVLPDARNPFFTDVARAAQERAREHDLALVLCDADSV
                     AAQEDEFLALLLERRLRGALITSVDYTNPRLWELPGLGLPIVLVDHIEPPDSPIPSVG
                     VDDVAGGRAAVAHLIELGHRRIAFVGTPGSMAQVTGRHAGALAALGDAGLPEDALVVV
                     KTDWLSAQDGRRAAARILELPAEERPTAVFCANDMLALGVLQEASLRGLAVPDDLAIV
                     GYDDIEFASIAAVPLTSVRQPRHDLGTIAVDLILAGDDVVDRHPVLQPELVVR"
     gene            1122..1364
                     /locus_tag="VFC06_01245"
     CDS             1122..1364
                     /locus_tag="VFC06_01245"
                     /inference="COORDINATES: protein motif:HMM:NF039417.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6364 family protein"
                     /protein_id="HZL80562.1"
                     /translation="MNTKLTLRLDDELIRAAKVHSEKSGRSVSKLVADFFALIDAQDR
                     PPNTEPTPRVRSLVGALKGATVDEDDYRRHLEEKHS"
     gene            1361..1789
                     /locus_tag="VFC06_01250"
     CDS             1361..1789
                     /locus_tag="VFC06_01250"
                     /inference="COORDINATES: protein motif:HMM:NF024861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HZL80563.1"
                     /translation="MRVLFDTNVVLDVLLAREPHLEDATRLFSLVDRGAIAGVVCATT
                     ITTIHYIASKALGPAAVAHLLGELLAMFDVAAVDLPVLRAALALGFTDFEDAVLHEAA
                     RAADAQAIVTRNVRDFAAAELPVFEPRELLAALDAAAGPN"
     gene            1896..>2234
                     /locus_tag="VFC06_01255"
     CDS             1896..>2234
                     /locus_tag="VFC06_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80564.1"
                     /translation="MELRDLGLDQGFADRALEQCDPDLSIARVTAVERGGHLVHGEQG
                     EIPAELTGRLQFVAGGSTDLPTVGDWVCVRDRDGGTQANIHAVLPRRSSLRRKVPGKD
                     IDFQLIAANID"
BASE COUNT          342 a          764 c          799 g          329 t
ORIGIN      
        1 cggaccacaa gctcgggctg gagcacgggg tggcggtcga cgacgtcgtc gccggcgagg
       61 atgaggtcga cggcgatggt gcccaggtcg tggcgcggct ggcggacgct ggtcaacggg
      121 actgccgcga tggacgcgaa ctcgatgtcg tcgtacccca cgatcgccag gtcgtccgga
      181 acggccagcc cgcgcaggct ggcctcctgc agcacaccga gcgcgagcat gtcgttggcg
      241 cagaagaccg cggtgggccg ctcctccgcg ggcagctcga ggatccgcgc agctgccctc
      301 cggccatcct gcgccgagag ccaatcagtc ttcacgacca cgagtgcgtc ctcggggagt
      361 ccggcgtcac cgagcgcggc cagcgctccg gcatggcgcc cggtgacctg ggccatgctc
      421 cccggggtgc ccacgaaggc gatccgccgg tgcccgagct cgatcaggtg ggcgacggcg
      481 gcacgcccgc cggcgacgtc gtccaccccg acgctcggga tgggggagtc cggcggttcg
      541 atgtggtcca cgagcacgat cggcagcccg aggccgggaa gctcccagag ccgggggttg
      601 gtgtagtcga ccgaggtgat gagggcaccg cgcaggcggc gttcgaggag gagggcgagg
      661 aactcatcct cctgggcggc gacggagtcg gcgtcgcaga gcacgagcgc gagatcgtgt
      721 tcacgggccc gctcctgggc agcccgcgcg acatcggtga agaacgggtt gcgcgcgtcc
      781 gggaggacgt aggcgagcag gcgactggtg ccggagcgca gctgcctcgc cgagtcgttc
      841 cgcacgaacc cgagctcccg gacggcctgc tcgaccttct cccgagttgc cggccggacc
      901 tggtccgggc gattgagcac gttggagacc gtgccgacag atacgccggc gcggcgcgca
      961 acgtcgacaa gcttcgctgt tcccacggac actcctcttt tgaaacgatt ctaggggatg
     1021 ttggccgtgc ggtcggcgaa gcgtcacggg gcgccgccct ccttgcttcc cactcttccg
     1081 gacgtacaat gtggactgat gctgtacgtc aggaggtccc ggtgaacacc aagctcacgc
     1141 ttcggctgga cgacgaactc attcgtgctg cgaaggtgca ctcagagaag tcgggcaggt
     1201 ctgtgtcgaa gctggtggcc gacttcttcg cgctcatcga cgcgcaggac aggcccccca
     1261 acaccgaacc cactccgcgc gtccgttccc tggtgggtgc gctgaagggc gccaccgtgg
     1321 acgaggacga ctaccgccgc cacctcgagg agaagcactc gtgagggtgc tgttcgacac
     1381 caacgtcgtg ctcgacgtgc tcctggcgcg cgaaccacac ctcgaggacg cgacgcggct
     1441 gttctcgctc gtggaccgcg gcgcgattgc cggcgtcgtc tgcgccacca ccatcaccac
     1501 gatccactac atcgcctcga aggcgctggg accggcggcc gtggcccacc tcctcggcga
     1561 gctcctggcg atgttcgacg ttgcagcggt ggaccttccg gtgctgcgcg ccgcgctcgc
     1621 gctcggattc accgacttcg aggacgccgt gctccacgaa gccgcccggg ccgccgatgc
     1681 acaggccatc gtgacgcgga acgtcaggga cttcgccgcc gctgaactgc cggtgttcga
     1741 accgcgcgag ctgctcgccg ccctggatgc cgcggccggc ccgaactgac cccgtcagcg
     1801 cggcgcccgc caggcgcgtg aggccgacgg agaatcgcgg gaggccggca gtaaatcagg
     1861 tgcggcggcg tggcaggctc tgccacagtg gcggcatgga attgcgcgac ctcggtcttg
     1921 accaggggtt cgccgaccgc gccctcgaac agtgcgaccc ggacctgtcc attgcgcggg
     1981 tgaccgcggt cgagcggggc ggccaccttg tccacggcga gcagggtgag attcctgcgg
     2041 agctgacggg ccgcctccag ttcgtcgccg gcgggtcaac cgacctgccg accgtgggcg
     2101 attgggtgtg cgtgcgcgat cgcgacggcg gcacgcaggc gaacatccat gccgtgctgc
     2161 cgcgccggtc gtccctgcgg cggaaggtgc ccggcaagga catcgacttc cagctgatcg
     2221 cggcgaacat cgac
//
LOCUS       DASRKH010000076         3065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_53729, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000076 DASRKH010000000
VERSION     DASRKH010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53729"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /gene="amrS"
                     /locus_tag="VFC06_01260"
     CDS             <1..1017
                     /gene="amrS"
                     /locus_tag="VFC06_01260"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HZL80565.1"
                     /translation="QCDLCPRHCRLHAGQRGFCFVRARDGDRMVLTTYGRSSGFAIDP
                     VEKKPLAHFYPGTSVLSFGTAGCNLNCRFCQNWDISKSREWDRLTSVASPDAIAAAAS
                     SSRCDSIAFTYNDPVIFAEYAIDTARACHERGIHTIAVTAGYISADARAEFFEPMDAA
                     NIDLKSFTEDFYWKVTGGHLRDVLDTIVWVHDHSDTWMELTTLLIPGHNDSNEEIARM
                     SQWILTELGPDVPLHFSAFHPDFKMLDIPPTPRATLRAARATALDAGLHFVYTGNMHD
                     PGGETTSCYACGHPLIERDWYEILAYRLTEDGRCPACGAAVPGRFGNVAGRWGRRSLP
                     VRMA"
     gene            complement(1027..1929)
                     /locus_tag="VFC06_01265"
     CDS             complement(1027..1929)
                     /locus_tag="VFC06_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZL80566.1"
                     /translation="MRATSIEELRMIAAVEAHGSLSAAAESLGVSQQAVSQRMRALER
                     RWSLPLFERTARGTTLTPHGALVAEWAGSLLAQVDAFDHAVLALRSDRAARVRVGASL
                     TIAEHLVPGWLVTYATEPHAARVELTVVNSATVVDRVRSGADDLGFIETPDVPEGLSV
                     RVIGHDEIGIVVSPRHPWAASASVSAAELAATPLVGREPGSGTRLALERALAAAVGAD
                     AIVEPAAELATTAGIRATLVAGGGVGALSLRAVRDDLDGGRLVRVKIDGLLVSRPLAA
                     IWHPGRPLLAGATRLLALASMAAL"
     gene            2029..>3065
                     /locus_tag="VFC06_01270"
     CDS             2029..>3065
                     /locus_tag="VFC06_01270"
                     /inference="COORDINATES: protein motif:HMM:NF015552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HZL80567.1"
                     /translation="MRIPVMTPHWFTAVMGTGIVGIAAHTLPVTSPALRDISVMAWAL
                     DVALGLTVVTLTAAHYLQDHGALRRYLLDPASAPFFGAMAMGILTMGTSTLLVGAEFI
                     GARSAADIDLTLWWIGTIIGVASASLVPVLAFTRHRVAPDAATPVWLLPVVAPMVSAA
                     SGALLLDHVHGTAAQALTIACYAQFGATAMAALAVTASVWNRLAHHQHRHAATAPALL
                     IVLGPLGQSVTAAGLLADHAPESLGLTRHAMTTFSILYGIPTMGFAMLWLIIAGVIVL
                     DAARRGLPFSLGWWSFTFPIGTCVTGATVLAHHTGAAAFTALAVALYALLLVGWSLAA
                     GGTVRMLAARWA"
BASE COUNT          437 a         1159 c          971 g          498 t
ORIGIN      
        1 cagtgcgacc tgtgtccccg tcactgccgc ctgcacgcgg gacagcgagg attctgcttc
       61 gtgcgcgcgc gggacggcga ccgcatggtg ctcaccacct acggacgcag ttccggcttc
      121 gcgatcgacc cggtcgagaa gaagccactg gcccacttct atcccggcac ctccgtgctg
      181 tcgttcggaa cggcgggctg caacctcaac tgccgcttct gccagaactg ggacatttcc
      241 aagtcccgcg agtgggaccg gctcacgtcg gtggccagtc cggacgcgat cgcggcggcg
      301 gcgtcctcct cgcgctgcga ctcgatcgcc ttcacgtaca acgacccggt gatcttcgcg
      361 gagtacgcga tcgacaccgc ccgcgcctgc cacgagcgcg gcatccatac gatcgcggtc
      421 accgccggct acatcagcgc cgatgcccgg gccgagttct ttgagcccat ggacgccgcc
      481 aacatcgacc tcaagtcctt caccgaggac ttctactgga aggtgacggg cggccacttg
      541 cgcgacgtgc tcgacaccat cgtctgggtg cacgaccaca gcgacacctg gatggagttg
      601 acgaccctcc tcattcccgg tcataacgac tcgaacgagg agatcgcgcg catgtcgcag
      661 tggatcctca ccgaactggg gcccgacgtg cccctccact tctcggcgtt ccaccccgac
      721 ttcaagatgc tcgacatccc ccccacgcct cgtgcgaccc tgcgtgcggc gcgtgccact
      781 gcgctcgatg ccgggctcca ctttgtctac accggcaaca tgcacgaccc ggggggcgaa
      841 acaaccagct gctacgcgtg cgggcacccg ctcatcgaac gcgactggta cgagatcctt
      901 gcctaccgcc tgacggagga cggtcgctgc ccggcctgtg gtgctgcagt tccgggacgc
      961 ttcggtaacg tcgccggacg ctggggtcga cgctcgctcc cggtccgcat ggcctgaggt
     1021 tggtccctag agcgccgcca tcgatgccag cgcaagcagg cgcgtcgcgc ccgccagtag
     1081 cggcctacct gggtgccaga tcgccgccaa gggcctgctc accaggagcc cgtcgatctt
     1141 cacacgcacc aggcgtccgc catccaaatc gtccctcacc gcgcgcaggc tgagcgcccc
     1201 cactcccccg cccgcgacga gggtggcgcg aatgcccgcg gtggtcgcga gttccgcggc
     1261 cggctcaacg atggcgtcgg cacccaccgc tgcggcaagc gccctctcga gggcgagtcg
     1321 ggtgccgctg cccggctccc tgccgaccag cggcgtggcg gcgagttccg ccgccgatac
     1381 cgacgcgctg gcggcccacg ggtgacgcgg cgagaccacg attccgatct cgtcatggcc
     1441 gatgacgcgc acggacaagc cttcgggcac gtcgggcgtc tcgatgaacc ccaggtcgtc
     1501 cgcgcccgaa cgcaccctgt ccaccacggt ggcgctgttg accaccgtga gttccacccg
     1561 cgcggcgtgc ggttcggtcg cataggtgac caaccacccc ggcaccaggt gttccgcgat
     1621 ggtgaggctc gcgccgacgc gcacgcgtgc cgcgcggtcg gacctcagcg ccagcaccgc
     1681 gtgatcgaac gcgtccacct gggccagcag ggagccggcc cactcggcga ccaaggcgcc
     1741 gtggggcgtg agcgtggtgc cccttgccgt gcgctcgaac aggggcagcg accagcgccg
     1801 ttccagcgcc ctcatgcgct gggagacggc ctgctgggag acgccgagcg actccgcggc
     1861 cgcactcaaa ctgccgtggg cttcgactgc cgcgatcatc cgcagttcct cgatcgaggt
     1921 agccctcatc gcactccctt gccgccagct ggcacctaca agcgtggctt gtggatcgcc
     1981 ctcgaagtca ctctaccgcc ggtccgcagc gaacgagacg atcgatgcat gcgcatcccc
     2041 gtcatgactc cccactggtt caccgccgtc atgggcaccg gcattgtcgg aatcgccgcc
     2101 cacaccctcc cggtcacctc gcccgcgttg cgggacatct cggtgatggc ctgggcgctg
     2161 gacgtcgcgt tgggactcac cgtcgtgacc ctcacggccg cccactacct ccaggaccac
     2221 ggcgcgcttc gccggtacct cctcgacccg gcatcggcgc cgttcttcgg cgcgatggcg
     2281 atggggatcc tcacgatggg aacctccacc ctgctggtgg gcgcggagtt catcggcgct
     2341 cggtcggccg cggacatcga cctaaccctg tggtggatcg gcacgatcat cggcgtggcc
     2401 tccgcctcgc tcgtgcccgt cctcgccttc acgcggcacc gagtggcacc cgacgccgcg
     2461 acgcccgtgt ggttgctccc cgtggtggcg ccgatggtgt cggcggcgag cggtgcgctc
     2521 ctgctcgacc acgtgcacgg cacggcggcg caagctctga cgatcgcctg ctacgcgcag
     2581 tttggcgcga ccgccatggc ggcactagcc gtgaccgcgt cggtgtggaa ccggctcgct
     2641 caccatcagc accgacacgc ggccaccgcc cccgcgctgc tgatcgtgct cggcccgctc
     2701 ggccagtccg tgacggcggc gggcctgctc gccgaccacg cgcccgagag tctcggcctc
     2761 acgcgccatg ccatgaccac gttctccatc ctctacggga tccccaccat gggttttgcc
     2821 atgctgtggc tcatcatcgc cggcgtgatc gtgctcgatg ccgctcggcg agggctgccg
     2881 ttctcgctcg gatggtggtc gttcaccttc cccatcggca cgtgcgtcac cggtgccacc
     2941 gtgctggcac accacaccgg cgcggcggcg ttcaccgcgc tcgccgtggc gctctacgca
     3001 ctcctgttgg tcggttggtc gctcgcggca ggcggcaccg tgcgcatgct ggccgcgcgg
     3061 tgggc
//
LOCUS       DASRKH010000077         5239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_14870, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000077 DASRKH010000000
VERSION     DASRKH010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 5239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5239
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14870"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /gene="ftsZ"
                     /locus_tag="VFC06_01275"
     CDS             complement(<1..980)
                     /gene="ftsZ"
                     /locus_tag="VFC06_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HZL80568.1"
                     /translation="MAAPQNYITAIKVVGVGGGGVNAVNRMIEVGLKGAEFIAINTDA
                     QALLMSDADVKLDIGRELTRGLGAGADPEVGRKAAEDHEDEIAEALRDADMVFVTAGE
                     GGGTGTGGAPVVARIARALGALTVGVVTRPFSFEGRRRFDQAEAGIENLRDEVDTLIV
                     IPNDRLLDIQDANLSVLGAFAAADQVLQGGVQGITDLITTPGLINVDFNDVKSVMKGA
                     GTALMGVSSARGENRALAAAEGAIASPLLEASIEGAHGVLLSIAGGSNLGIKEVETAA
                     KLVREAAHPEANIIFGTVIDDALGDELRITVIAAGFDGGAPPVRRHLGALG"
     gene            complement(1104..1937)
                     /locus_tag="VFC06_01280"
     CDS             complement(1104..1937)
                     /locus_tag="VFC06_01280"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HZL80569.1"
                     /translation="MNTASVPVRGRGEDAPAWQPVKQRERADTVTHRMRERLAERRRT
                     ERRGAAVKWGRYGLAAGVVVLIVWLVLRSPVFSLDPAKVELSGVTPEVDATAIHMVLA
                     QYDGSSLALLNVPHVADQLRDLVGVQDATVERVWPAGLRVTITPRHPVAAIASGGGFV
                     LLDADAVKVGESEVAPANLPVLNIPLGTDNDRILDAVLEVVRNLPQDVLDRVESVGAQ
                     TEDTVAFTLRDGPRVEWGSSQDSALKAQVLGVMLTSGAASTADVIDVSAPTLPITRKQ
                     D"
     gene            complement(2149..3513)
                     /gene="murC"
                     /locus_tag="VFC06_01285"
     CDS             complement(2149..3513)
                     /gene="murC"
                     /locus_tag="VFC06_01285"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HZL80570.1"
                     /translation="MRFHFIGVGGSGMSAVARLVAAKGVSVTGTDKRRSAYFAALEDA
                     GVDVRVGHDAALVDGADVVVISTAVRDSNEELARARALGLEVWHRSKALAWALEGRRL
                     VSVAGSHGKTTTSAMVAHMLHRCGVDASYAVGARVFGVEGAVAGGYGGASDIAVVEAD
                     ESDGSFLNYDTEIAIVLNVEPDHLDFFGSFAAVEEAFVRFASRARVLIACIDDPGAAR
                     VADAAAEAGVRVVRYGVWPRPPGARDADVVVSDRDVTWAERAMPLVVPVPGVHQRLNA
                     SAAWAAAVELGIDPVRAADAVASFAGTGRRFELRGEARGMRVIDDYAHHPTEVAAVLA
                     AARADGAGRLVVLFQPALFSRTQALGAEFGRALSIDNAAIILAPVYGDREDPIPGVTS
                     DVIADALTAGDGSTVELADSLEDAADRAAALARPGDTVMTIGSGTVTQAAGWILDKWS
                     RADG"
     gene            complement(3510..4628)
                     /gene="murG"
                     /locus_tag="VFC06_01290"
     CDS             complement(3510..4628)
                     /gene="murG"
                     /locus_tag="VFC06_01290"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HZL80571.1"
                     /translation="MARVLLAGGGTAGHVNPLLATADALLQRGHDVAALGTEGGLEAH
                     LVPAAGIELHFVPRVPLPRRPSLDLLRLPVRLGAAIAAAGRAIDAIDADVVVGFGGYV
                     STPAYLAARRRKVPVVVHEGNARPGLANRLGSRWAAAVALTFEGTPLKGGVVTGLPMR
                     PAIIDLAQLLRDPVTGDPMRARARAAWGWAPDAPTLLITGGSTGAASLNAAAADVVER
                     LVQHGIHVLHLTGKGKSDGAERARGNLSARHREMYVIREYVEDMETAFAAADAVVCRA
                     GANTVCEISALGLPAVYVPLPHGNGEQALNAKGAVDAGAATIVHDSELTAAALELVAE
                     PLVLDRKARDAARTAAESIGVVDGAVRLAEIIERVARR"
     gene            complement(4621..>5239)
                     /locus_tag="VFC06_01295"
     CDS             complement(4621..>5239)
                     /locus_tag="VFC06_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HZL80572.1"
                     /translation="FAVGAAAIVAGGLALISQGDTRSARISVWLDPSTCDIQDNCLQT
                     THGTWALASGGLWGLGPGMSREKWGYLPAADNDFIFAILGEEFGLIGTLLVLIAFAMI
                     VIAVSRIVRRHRDPFAQIAAGAIGAWIVGQAVINIAVVLELLPVTGVPLPLVSSGGSS
                     LVMSLTALGALMAFARSEPGAREAFSARPSVIRRSMAVLSGGRRG"
BASE COUNT          846 a         1873 c         1745 g          775 t
ORIGIN      
        1 cccaacgcgc ccaggtggcg gcgcactggc ggggctcccc cgtcgaatcc cgccgcgatg
       61 acggtgatgc gaagttcgtc gcccagggcg tcgtcgatga ccgtaccgaa gatgatgttc
      121 gcctcggggt gagcggcctc gcgtaccagt ttggctgcgg tctcgacttc cttgattccg
      181 aggttggagc cgccggcgat gctcaacagc actccgtgag caccctcgat cgacgcctcg
      241 agcagcgggg acgcgatggc gccttcggcg gccgcgagcg cgcggttctc gccgcgagcg
      301 ctggagacgc ccatgagggc ggtgcctgcg cccttcatga cggacttcac gtcgttgaag
      361 tcgacgttga tgaggccggg ggtggtgatg aggtcagtga tgccctggac gccgccttgg
      421 agaacctggt cggccgccgc aaaggccccg agcacggaga ggttggcgtc ctggatgtcc
      481 agaagacggt cgttgggaat cacgatgagc gtgtcgacct catcgcgcaa gttctcgatg
      541 ccggcttccg cctggtcgaa ccggcgtcgt ccctcgaagc tgaacggtcg cgtcaccacg
      601 ccgacggtga gcgcgccaag cgccctggcg atgcgtgcca cgacgggcgc gccgccggtg
      661 ccggtgcctc cgccctcgcc tgccgtcacg aacaccatgt cggcgtcgcg aagggcctca
      721 gcgatctcgt cttcgtggtc ctcggcggct ttgcggccca cctcggggtc cgcgcctgcg
      781 ccaaggccgc gggtgagttc gcggccgatg tcgagcttga cgtcagcgtc ggacatcagc
      841 agcgcttggg cgtcggtgtt gatggcgatg aactcggcgc ccttgaggcc cacctcgatc
      901 atcctgttga cggcgttgac tccgccgccc ccgacgccga cgaccttgat cgccgtgatg
      961 tagttctgcg gtgcggccat ggcagtccct tcaacattaa ggttcaactt gagggttagg
     1021 gttatgtcaa gctgatctgc aatgacggta cgtgggccga gcccccgcgg gccgaattgg
     1081 cgcgcgtgtc gcgccagaat cccctaatcc tgtttccgcg taatcgggag cgtcggcgct
     1141 gacacgtcga tgacgtcggc cgtggaggcc gctcctgacg tcaacatgac gccgagcact
     1201 tgagccttca acgccgagtc ctgggaactc ccccactcga cgcgcggacc gtcacgcagg
     1261 gtgaacgcta cggtgtcttc ggtctgtgcc cccaccgatt ccacgcgatc caacacgtcc
     1321 tgaggcagat tgcgcaccac ctcgagcacg gcatcgagga tgcgatcgtt gtcggtcccc
     1381 aacgggatgt tgagaaccgg aagattcgct ggcgcgacct ctgactcgcc caccttcacg
     1441 gcgtcggcat cgagcaacac gaaccccccg ccgctcgcga tcgccgccac cggatgacga
     1501 ggcgtgatcg tgacgcgaag acccgcgggc cacacgcgct ccacggtcgc atcttgaaca
     1561 cccacgaggt cacgcaactg gtccgcgacg tgcgggacgt tgaggagcgc aagggaggag
     1621 ccgtcgtact gcgcgaggac catgtgaatc gccgtggcat cgacctcggg cgtcacgccc
     1681 gatagttcga ccttggccgg atcgagcgaa aacacgggcg accgaagcac gagccagacg
     1741 atcagcacca ccacaccggc ggccagcccg taacgacccc acttcacggc ggcgccgcgg
     1801 cgctcggtgc ggcggcgttc cgcgagccgc tcccgcatcc gatgcgtgac ggtgtcggcc
     1861 cgctcgcgct gcttgaccgg ttgccaggcc ggggcatcct cgccccgacc cctcaccggt
     1921 accgaggccg tgttcacgcg ggacgccgca cgtttcggct cgggtcgcgg ggcaactggc
     1981 gaccgacgga ccggggtctg gcggtcggca gcgggcttgc gcggctccgc cttggcgctc
     2041 cgggtcggtt gagaacgctt ctccggttgc tggcgccttt ccgtgggctg atcgcgacgc
     2101 cgctgcgccg tttgggaacg ctgggtcggc acgctcgggg gccgtcgctc acccatctgc
     2161 ccggctccac ttgtcaagaa tccagcccgc ggcctgcgtc acggtgccgg aaccgatcgt
     2221 catgacggtg tctcccggac gcgccagcgc cgctgccctg tccgcggcat cctcaaggct
     2281 gtcggccagc tcgacagtgg acccgtctcc cgctgtgaga gcgtccgcga tgacgtccga
     2341 tgtcacgccc gggatcgggt cctcacggtc cccgtacacg ggggccagga tgatggcggc
     2401 attatcgata gacaaggcgc gcccaaactc cgcacccagc gcttgggtcc ggctgaagag
     2461 cgcgggctgg aacaacacga ccaaccggcc agcaccgtcc gcgcgcgccg cagcgagcac
     2521 ggccgccacc tccgtggggt ggtgcgcgta gtcgtcgatg acccgcatgc cgcgggcctc
     2581 tccgcgcagt tcgaaacgcc ggccggttcc cgcgaaactc gccacggcat cggccgcgcg
     2641 caccgggtcg atgcccagtt ccaccgccgc cgcccacgcc gccgacgcgt tgagccgttg
     2701 atgcaccccg ggcacaggaa cgacgagcgg catggcgcgc tcggcccagg tcacgtcgcg
     2761 atcgctgacc accacgtccg cgtcgcgcgc gcccggcggt cgcggccaga cgccatatct
     2821 caccacccgc acccctgcct cggctgctgc gtccgctacg cgggccgcgc cgggatcgtc
     2881 gatgcaggcg atcagcacgc gggcgcggga ggcgaaacgc acaaaggcct cctcgaccgc
     2941 cgcgaacgat ccaaagaagt ccaggtgatc cggctcgacg ttgagcacaa tggcgatctc
     3001 agtgtcgtag ttcaggaagg acccatccga ttcgtcagcc tcaaccacgg cgatgtccga
     3061 cgcaccgccg tagcctccgg cgacggcgcc ctcgactccg aacacccgag cccccactgc
     3121 gtagctggca tccaccccgc accggtgcag catgtgcgcc accatggccg atgtggtggt
     3181 cttgccgtgc gatccggcga ccgagacgag gcggcgccct tcgagcgccc aggcgagcgc
     3241 cttggaccgg tgccacacct caagtcccag ggcgcgcgcc cgcgcgagct cctcattgct
     3301 gtcgcgcacc gcagtagaaa tcaccaccac gtcggcgccg tcgacgagcg cggcgtcgtg
     3361 gcccacgcgc acatccacgc ccgcgtcctc gagcgcggca aagtacgcgc tacgacgctt
     3421 gtcggtgccg gtcacggaga cgcccttggc ggccacgagg cgcgccacgg cggacatgcc
     3481 cgagcctccc acgccgatga agtggaatct catcgtcgtg cgacccgttc gatgatctcg
     3541 gcgaggcgca cggcgccgtc caccaccccg atcgactctg ccgcggtgcg cgccgcgtcg
     3601 cgcgccttgc gatccagcac gagcggttcg gcgacgagct caagggccgc cgcggtcagt
     3661 tcgctgtcat gcacgatggt cgccgcgccc gcgtccaccg ctcccttggc gttgagcgcc
     3721 tgctcgccgt tgccgtgggg aagcggaacg tagaccgcgg gcagccccag ggccgaaatc
     3781 tcgcacacgg tgttcgcgcc agcgcggcac acgacggcgt cggccgcagc gaatgcggtc
     3841 tccatgtcct cgacatactc gcgaatcacg tacatctcac ggtggcgggc cgagaggttg
     3901 ccgcgcgccc gctcggcgcc gtcggacttg cccttgccgg tcaggtgcag cacgtgaatg
     3961 ccgtgttgga ccagccgctc gaccacgtct gcggcggcgg cgttgaggct cgcagcgccc
     4021 gtggagccgc cggtgatgag cagggtcggc gcgtcggggg cccagcccca cgctgcgcgc
     4081 gcacgcgctc gcatggggtc acccgtgacg gggtcgcgca agagctgtgc gagatcgatg
     4141 atcgctggcc gcatgggcag cccggtcacg actccgccct tgagaggcgt gccctcaaac
     4201 gtcagcgcca cggcggcggc ccagcgagag ccgaggcgat tggcaagacc gggtcgcgca
     4261 tttccctcgt gcaccacgac gggcacctta cgtcgacgtg ctgccaggta ggcaggagtc
     4321 gacacgtagc ctccaaagcc gaccaccacg tcggcgtcga tggcatcgat cgcgcggccg
     4381 gccgccgcga tcgcggcacc caatcgcacg ggcaagcgca acaggtcgag cgacggcctg
     4441 cgcggtaacg gcacgcgcgg cacaaagtga agttcgattc ccgcggccgg caccaggtgg
     4501 gcctcgagtc ctccctctgt acccagggcc gcgacgtcgt gtccgcgctg cagcagggca
     4561 tcggccgttg ccagcagcgg attgacgtgg cccgcagtgc ccccgccagc caacagcacc
     4621 ctagccacgg cgacctccgg acaacacggc catggagcgg cggatgacgg acgggcgcgc
     4681 cgagaacgcc tcgcgagctc cgggctcgct gcgcgcgaag gccatcagtg ctccgagcgc
     4741 ggtcagcgac atcacgagcg aagaacctcc ggacgacacc aacgggagtg gcactcccgt
     4801 gacgggaagc aactcgagca ccaccgcgat gttgatcacg gcctgaccca caatccacgc
     4861 tccgatcgcg cccgcggcga tctgcgcgaa ggggtcgcgg tgccgccgca caatgcggct
     4921 cacggcaatc actatcatgg cgaaggcgat caggacgagc agggtgccga tgaggccaaa
     4981 ctcctcgcca aggatcgcga agatgaagtc gttgtcagcg gcaggaagat atccccactt
     5041 ctcgcggctc atgccggggc cgaggcccca caagccgccc gaagcgagcg cccacgtgcc
     5101 atgcgtcgtc tgcaggcagt tgtcctgaat gtcgcaggtc gacgggtcga gccagacgct
     5161 gatgcgcgcc gagcgcgtgt cgccttgaga aatcagcgcg agcccaccag cgacgatggc
     5221 cgccgccccg accgcaaag
//
LOCUS       DASRKH010000078         4288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_23647, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000078 DASRKH010000000
VERSION     DASRKH010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23647"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1119)
                     /locus_tag="VFC06_01300"
     CDS             complement(<1..1119)
                     /locus_tag="VFC06_01300"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007588760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrophosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HZL80573.1"
                     /translation="MVRRVALLTAGGFAPCLSSAVGGLIERYTEVAPEAEIIAYQYGY
                     HGLLTGHHVVVDGEARRNAAVLHRFGGSPIGNSRVKLTNVKNLVSRGLVREGENPLEV
                     AAEQLRRDGVDILHTIGGDDTNITAADLAEFLENNGYHLTVVGLPKTIDNDIVPIRQS
                     LGAATAVEETALFAQNVIAEHGSSPRMLIVHEVMGRNCGWLTAAASRKYREWLDEQAW
                     VPSIGLTRERWEIHAIYVPEMAPNLDAEARRLRTVMDELGAVNIFLSEGAGVPEIIAE
                     MEAAGEAVPRDPFGHVRLDAINPGRWFASQFAEHIGADKVMVQKSGYFARSSRANEED
                     LALIRIMIDFAVEAALRGESGVIGHDEENGDELSVIAFP"
     gene            complement(1168..2091)
                     /locus_tag="VFC06_01305"
     CDS             complement(1168..2091)
                     /locus_tag="VFC06_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZL80574.1"
                     /translation="MLYGFVKATLGSALKVFYQPWIRGQQNIPSDGPAILASNHLAVI
                     DSFFLPLMIRREVVFMGKSDYFTGTGIKGKAVAAFMRGVGTIPVDRSGGKASEAALRT
                     GLKRLAEGELFGIYPEGTRSPDGRLYRGKTGVARLALESGAPVIPVAMIGTNIAQPIG
                     RKIPKLHRIGVVVGEPLDFSRYAGMHGDRFVLRSITDEIMYELMRLSGQEYVDIYAAT
                     MKNRMAAAKKEAAKKEKDESVAKPPERVGAPGGRPVPEVEVPQPTEDEIREGLERLEA
                     ARTEMAARGEELHIDNPDSADESGEPDTTSE"
     gene            2179..2580
                     /locus_tag="VFC06_01310"
     CDS             2179..2580
                     /locus_tag="VFC06_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80575.1"
                     /translation="MARTPGDFDPDELDARWNDLTSRLGPLDGTRAIPVEAAPQSPGE
                     LARGPRDYEVPEDDSGFEEPDPELGNPDPATALSWFALGIGVLGLFVVVIAGSPPWVG
                     VLSTALTVGGLVALLVRLPRHTDRDDDGAQV"
     gene            2734..>4288
                     /locus_tag="VFC06_01315"
     CDS             2734..>4288
                     /locus_tag="VFC06_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HZL80576.1"
                     /translation="MIEFHTPQAVHVSADTSISDYLIRYGTERPASTMFRQKVGETWQ
                     DWSAARALGVVRGIAQGLRAAGYGPGDRIAIMSRTRLEWTLADFAIWHIGGIPVPIYE
                     SSSPSQAQWILFDSGAVAAFVETAALAAIVEEARAEDDGHSVRDVWVIDDGAFETLAA
                     QGASLGDATADDAVAAVGLSDLATIIYTSGTTGRPKGVELSHGNFVELCEEVVNLMPE
                     IFLEPDASTLLFLPLAHVFARVVEVVMVIGKIPMGHTPDARQAVADISVFKPTVFLSV
                     PRVWEKIYNSAELKQGKGLKRSIFRRAAKISVSYSRSLEAPGGPSALLKVQHAIADRL
                     VYSKLRELLGGQLHWTISGGAPLGERLGNFYQGIGLHMLEGYGLTETTAPTHINLPKK
                     TKIGTVGPPLPGTTIRIAEDGEILAKGIGVFRAYHNNPAATAEALEGGWFHTGDVGHM
                     DEDGYLAITGRQKELIVTAGGKNVAPAQLEDPLRTHPLISQAVVIGDNQPFIAALITL
                     DAEVLPRWLE"
BASE COUNT          654 a         1509 c         1435 g          690 t
ORIGIN      
        1 ggggaaggcg atgacggaca gttcgtcgcc gttctcctcg tcgtgcccga tgaccccgga
       61 ctcgccccgc agggcggcct cgaccgcgaa gtcgatcatg atcctgatga gggcgaggtc
      121 ctcctcgttc gcccgcgacg agcgggcgaa atagccggac ttctgcacca tgaccttgtc
      181 cgcgccgatg tgctccgcga actgactcgc gaaccagcgc cccgggttga tggcgtccag
      241 ccggacgtgc ccgaacgggt cgcgcggcac ggcctccccg gcagcctcca tctccgcgat
      301 gatctcgggg acgcccgcgc cctccgacag gaagatgttc accgcaccga gttcgtccat
      361 gaccgtccgg agccggcgcg cctcggcatc gaggttcggc gccatctcgg ggacgtagat
      421 ggcatggatc tcccaccgct ccctggtcag gccgatcgat ggtacccagg cctgctcgtc
      481 gagccactcg cggtacttgc gggacgcggc cgcggtgagc cagccgcagt tgcggcccat
      541 gacctcgtgc acgatcagca tgcgcgggct tgagccgtgc tcggcgatca cgttctgcgc
      601 gaagagcgct gtctcctcca ccgccgtcgc cgcccccagt gactggcgga tcgggacgat
      661 gtcgttgtcg atggtcttgg ggagccccac cacggtgagg tggtagccgt tgttctccag
      721 gaactcggcg aggtccgcag cggtaatgtt cgtgtcatcg ccgccgatgg tgtgcaggat
      781 gtccacgccg tccctgcgga gctgttcagc cgccacttcg agcgggttct ccccctcccg
      841 gaccagaccc cgggagacga ggttcttcac gttggtgagc ttcactcggg agttgccgat
      901 cgggctgcca ccgaagcggt gcagcacggc tgcgttcctc cgggcctcgc cgtcgaccac
      961 aacgtggtgg ccagtgagga ggccgtggta gccgtactgg taggcgatga tctccgcctc
     1021 gggcgccact tccgtgtacc gctcgatgag accgcccacg gcggatgaca ggcacggggc
     1081 gaatcccccg gcggtgagca aagctacgcg acgaaccatg tctcgcctct cgtgatcttc
     1141 cgggcgcccg caagcgccct tgccagttta ttcggacgtg gtgtccggtt ccccggactc
     1201 gtccgcggaa tccgggttgt cgatgtggag ttcctcgccc cgcgccgcca tctcggtgcg
     1261 cgccgcttcg aggcgctcca ggccctcgcg gatctcatcc tcggtgggct ggggcacctc
     1321 gacctcgggg accgggcgcc caccgggtgc gcccacgcgc tcggggggct tcgccacgga
     1381 ctcgtccttc tccttcttcg cggcctcctt cttcgcggcg gccatgcggt tcttcatggt
     1441 cgcggcgtag atgtccacgt actcctggcc cgagagccgc atgagttcgt acatgatctc
     1501 atccgtgatg gagcggagca cgaagcggtc accgtgcatc ccggcatagc gcgagaagtc
     1561 gagcggttcg cccaccacca cgccgatccg gtggagcttc gggatcttgc gcccgatcgg
     1621 ctgggcgatg ttggtcccga tcatcgcgac ggggatgacg ggtgccccgg actccagggc
     1681 gagccgcgcc accccggtct tcccgcggta gaggcggccg tccgggctgc gggtgccctc
     1741 gggatagatc ccgaacaact ccccctcggc gagccgcttg agaccggttc gcagtgcggc
     1801 ctccgaggcc ttcccgcccg agcggtccac ggggatggtc ccgacgcccc gcatgaaggc
     1861 cgcgaccgcc ttgcccttga tgccggttcc cgtgaagtag tccgacttgc ccatgaagac
     1921 cacctcgcgg cggatcatca gcgggaggaa gaaggagtcg atcaccgcga ggtggttgga
     1981 ggccaggatg gcggggccat ctgacgggat gttctgctgg ccccggatcc acggctggta
     2041 gaacaccttc agcgcgctgc cgagcgtcgc cttcacgaag ccgtagagca cgtcggccac
     2101 ctccgattga tgtcatcctg cgtctcaggg acgggccaaa ggttacagca ccgggcacac
     2161 gcgcctagag tcggggccat ggcacgcacc cccggcgact tcgatcctga tgagctcgat
     2221 gcccggtgga acgatcttac gtcccggctc ggcccgctgg acggaacacg ggcgatcccc
     2281 gtcgaggccg cgccccagtc cccgggggag ctcgcccgcg ggccgcgcga ctacgaggtg
     2341 ccggaggacg actccggctt cgaggaaccg gacccggaac tcggcaaccc tgacccggcg
     2401 acggccctca gctggttcgc gctggggatc ggcgttctgg ggctcttcgt cgtcgtcatc
     2461 gccggctccc ccccgtgggt gggggtgctc agcacggctc tcacggtggg tggtctggtc
     2521 gcgctcctcg tccgcctgcc ccggcacacc gaccgggacg acgacggcgc gcaggtctga
     2581 ggcttccgtc cgccactcct gaggcgccga tcgcccacgg cactgggcga tccgcacagg
     2641 ttcggtcctc ggtgtccggc ctcgccgggt gcgacgaggg tcacactccc cggtaggctt
     2701 cgatgaacgc atgaggcacc gaaggagccg ttgatgatcg aattccacac cccgcaggcc
     2761 gtgcacgtca gtgcggacac atcgatcagt gactacctca tcaggtacgg caccgagcgc
     2821 ccggccagca ccatgttccg gcagaaggtc ggcgagacgt ggcaggactg gtcggcggcc
     2881 cgggccctcg gcgtcgtgcg cggcatcgcc cagggcctgc gcgccgcggg gtacggcccc
     2941 ggcgacagga tcgcgatcat gtcccgcacc cggctggagt ggaccctggc cgacttcgcg
     3001 atctggcaca tcggcggcat tcccgtgccc atctacgaat cgtcgtcgcc ctcccaggcg
     3061 cagtggatcc tgttcgactc cggagctgtg gccgccttcg tcgagaccgc agcgctcgcg
     3121 gcgatcgtcg aggaggcacg cgctgaggac gacggccact ccgtgaggga cgtctgggtc
     3181 atcgacgacg gggcgttcga gacgctggcc gcgcagggcg cgagcctcgg ggacgcgacc
     3241 gccgacgacg ccgtcgccgc cgtcggcctc tccgacctgg cgacgatcat ctacacctcc
     3301 gggaccaccg gacgccccaa gggcgtggag ctctcccacg ggaacttcgt ggagctgtgc
     3361 gaagaggtcg tcaacctgat gccggagatc ttcctggagc ccgacgcctc caccctcctc
     3421 ttcctgcccc tcgcacacgt gttcgcccgc gtggtcgaag tggtcatggt gatcgggaag
     3481 atcccgatgg gccacacgcc cgacgcgagg caggccgtgg ctgacatctc ggtgttcaag
     3541 cccacggtgt tcctcagcgt cccgcgcgtg tgggagaaga tctacaacag cgccgagctg
     3601 aagcagggca agggactgaa gcgctccatc ttccggcggg cggcgaagat ctccgtgagt
     3661 tactcgcgca gcctcgaggc tcccgggggg ccgagcgcgc tcctgaaggt ccagcacgcg
     3721 atcgcggaca ggttggtcta ctccaagctc cgtgaactgc tcggcggaca gctccactgg
     3781 accatctccg gcggggcgcc gctcggtgag cggctcggga acttctacca gggcatcggc
     3841 ctgcacatgc tggagggcta cgggctgacc gagaccaccg cgcccaccca catcaacctg
     3901 ccgaagaaga cgaagatcgg caccgtcggc ccgccgttgc ccgggacgac gatcaggatc
     3961 gcagaggacg gcgagatcct ggccaagggc atcggggtgt tccgggccta ccacaacaac
     4021 ccggcggcca ccgccgaagc cctcgagggc ggctggttcc acaccggcga cgtcggccac
     4081 atggacgagg acggctacct cgcgatcacc ggccgccaga aggaactgat cgtcacggcc
     4141 ggcgggaaga acgtcgcccc ggcccaactg gaggacccgc tgcgcacaca ccccctcatc
     4201 tcgcaggccg tcgtgatcgg ggacaaccag ccgttcatcg cggcactcat caccctcgac
     4261 gccgaggtgc tcccgcggtg gttggaga
//
LOCUS       DASRKH010000079         2037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_6579, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000079 DASRKH010000000
VERSION     DASRKH010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6579"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="VFC06_01320"
     CDS             complement(<1..900)
                     /locus_tag="VFC06_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HZL80577.1"
                     /translation="MTQPAAPVPASDFVRDLVRQDMQRGTYGGRVQTRFPPEPNGFLH
                     IGHAKAITLDFSIAAEFGGTCNLRFDDTNPDTEEIHFVEGITEDVEWLGFRPAGVFHA
                     SDYFGQLYEWAEHLVMRGLAYVDDQDGDAISAQRGGFGKPGIESPFRDRGPEENLDLF
                     RRMRAGEFADGSRVLRARIDMNHENMQLRDPIMYRIRRGHHFRTGDEWCIYPTYDWAH
                     GQSDAFEGVTHSFCTLEFDSHRPLYDWFLEHLPLPSDQPRQTEFARLELTHTVMSKRL
                     LAALVADGVVDGWDDPRLPTLRGL"
     gene            complement(969..1053)
                     /locus_tag="VFC06_01325"
     tRNA            complement(969..1053)
                     /locus_tag="VFC06_01325"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1017..1019),aa:Leu,seq:tag)
     gene            complement(1077..1544)
                     /gene="bcp"
                     /locus_tag="VFC06_01330"
     CDS             complement(1077..1544)
                     /gene="bcp"
                     /locus_tag="VFC06_01330"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HZL80578.1"
                     /translation="MPLEVGQPAPPFTLATPGGEVSLSELVSGAGTGVVVYFYPAAMT
                     PGCTTEACDFRDTLNSFQGSGYTVVGISPDQPARLATFAEKEGLNFTLASDPDHAVAA
                     AYGAWGEKRLYGKVSVGLIRSTFVVAKDGTVSHVYRNVKATGHVERLRRDLVG"
     gene            1664..1739
                     /locus_tag="VFC06_01335"
     tRNA            1664..1739
                     /locus_tag="VFC06_01335"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1697..1699,aa:Lys,seq:ctt)
BASE COUNT          323 a          667 c          722 g          325 t
ORIGIN      
        1 cagcccgcgg agcgtgggca ggcgggggtc gtcccagccg tccacgacgc cgtcggccac
       61 gagcgcggcg agcagccgct tcgacatgac cgtgtgcgtg agctcgaggc gcgcgaactc
      121 ggtctgccgc ggctggtccg agggcaacgg gaggtgctcg aggaaccagt cgtacagggg
      181 ccggtggctg tcgaattcga gcgtgcagaa ggagtgggtg acgccctcga aggcgtcact
      241 ctgcccgtgc gcccagtcat acgtcgggta gatgcaccac tcgtcgccgg tgcggaagtg
      301 gtgcccgcgg cggattcggt acatgatcgg gtcccgcagc tgcatgttct cgtggttcat
      361 gtcgatgcgg gcccgcagca cccgcgaacc gtcggcgaac tcccccgccc tcatccggcg
      421 gaacaggtcg aggttctcct cggggccgcg gtcgcggaac gggctctcga tgccgggctt
      481 gccgaagcca cctcgctgcg ccgagatggc gtcgccatcc tggtcgtcga cgtaggccag
      541 gccccgcatg accaggtgtt cggcccattc gtacaactgc ccgaagtagt cggacgcgtg
      601 gaagaccccg gcgggccgga atcccagcca ctcgacgtcc tcggtgatgc cctcgacgaa
      661 gtggatctcc tcggtgtcgg gattcgtgtc gtcgaagcgg aggttgcacg tgcccccgaa
      721 ctcggcggcg atcgagaagt ccagggtgat cgccttggcg tggccgatgt gcaggaagcc
      781 attgggttcc ggggggaagc gcgtctgcac ccggccaccg taggtgcccc gctgcatgtc
      841 ctgacggacg aggtcacgga cgaagtcgct ggcggggacg ggagcggctg gctgggtcac
      901 gatcgaattt agcacgtcgg gacaggcctt cccgaactgc ccgtgccggc cgcccgccat
      961 gccggcagtg gtgcgcgaga cgggacttga acccgtacgt ccgaggacac tggaacctaa
     1021 atccagcgcg gctgccaatt acgccactcg cgcccgcccg ccaattctag agggagtcag
     1081 ccgacaaggt cgcggcgcag ccgctcgacg tgcccggtcg ccttcacgtt gcggtacacg
     1141 tggctcacgg tcccgtcctt cgcgaccacg aaggtggagc ggatcaggcc gacgctcacc
     1201 ttcccgtaga gcctcttctc gccccacgcc ccgtaggccg cggcgacggc gtggtccggg
     1261 tccgaggcca gcgtgaagtt gaggccctcc ttctcggcga acgtcgcgag ccgggcgggc
     1321 tggtcaggcg agatgccgac gacggtgtag ccgctcccct ggaacgagtt gagggtgtcc
     1381 cggaagtcac aggcctccgt ggtgcacccg ggcgtcatcg ccgcggggta gaagtacacg
     1441 acaacgccgg tcccggcccc ggagaccagc tccgacaggg acacctcgcc gcccggcgtc
     1501 gcgagggtga acggtggcgc gggctgtccg acctcaagtg gcatgagtgc tcctcttctc
     1561 gccgtccacg gtacaacatg gatgatccga agatcacagt gcgggggcac cccgggcatt
     1621 tgtcgcgtgg gcccaacgct ggtaacgtct ctggagccgc aatgcgccat tagctcaatt
     1681 ggcagagcag ctgactctta atcagcgggt tcggggttca agtccccgat ggcgtaccag
     1741 tgaggggccg gggatcacgc ccggcccctt ttttgcgccc agcggtcacc cacaatggca
     1801 tgtcgtggga ctggccggcg tgagaaggtg ggaccatccc gacgcgatgg aggcgagcgt
     1861 ggctggactg gcgagcggcg accaggggcc gtggctgacc ccgtacgatc ttgacgacgt
     1921 acgccgcaag gtgcccatgg tctacatcga ggccgtgccg ctgcggctcg gtccggacgg
     1981 cgacctcgag ttcgtgggca tgctgctggg cgccacccac gagagcgggc tgcagcg
//
LOCUS       DASRKH010000080         3801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_9988, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000080 DASRKH010000000
VERSION     DASRKH010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9988"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VFC06_01340"
     CDS             complement(<1..334)
                     /locus_tag="VFC06_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HZL80579.1"
                     /translation="MFTGIVQSVGTVTSIEDGGATARLTISSAGLGDDLVHGESIAVN
                     GVCLTVATLYTDTWAADVMRITLETSMLGDLTEGSRSTLNAPCSLPGGLEATSCRGTS
                     MEWPRWSRA"
     gene            complement(334..645)
                     /locus_tag="VFC06_01345"
     CDS             complement(334..645)
                     /locus_tag="VFC06_01345"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HZL80580.1"
                     /translation="MDKIVAEPHRGDGQSRRRAAILGSRQARTVIVEGGRHILTAFMR
                     AGLVDEINVYIAPALLGGGIAAITDLGIASMADALRGEDVTVTTLGVDCLVTAHMTKG
                     K"
     gene            619..1128
                     /locus_tag="VFC06_01350"
     CDS             619..1128
                     /locus_tag="VFC06_01350"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF006767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HZL80581.1"
                     /translation="MGLGDDLVHGESIAVNGVCLTVATLDTDTWTADVMRITLETSML
                     GDLNEGSSVNLERAMLATGRLGGHIMQGHVDGVATLVARDSQADWDDLTFALPLALLR
                     YVVRKGSIALNGVSLTVAELEGDRATVSLIPTTLTGTTFGDYAVGDKANVEVDVIAKY
                     VERMMGDHS"
     gene            1125..2366
                     /gene="ribB"
                     /locus_tag="VFC06_01355"
     CDS             1125..2366
                     /gene="ribB"
                     /locus_tag="VFC06_01355"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HZL80582.1"
                     /translation="MTDATIALVERALADLEAGKPVLVCDSHDREDEADFIMSAERVT
                     ADWVAWGIRHSSGYLCAPMQSSKADALHLPLMVPHSQDPRRTAYTVSVDAASGVTTGI
                     SAADRHHTLQVLSRADAKPDDVIRPGHVLPLRAVPGGVLHRAGHTEAAVDLCRLAGLE
                     PVAVIAELVNDDGTMMRQGDATALADKEGLILMTIADLIYYRRAKKDLPLHIEPQPHR
                     VLLEGEARLPTVHGEFTVKAYRDARTGDEHLAVYKEPTGAEVPIVRVHSECLTGDAFS
                     SLRCDCGPQLDAALVRVQAAGGAVVYLTGHEGRGIGIISKIQAYALQDAGRDTVDANL
                     DLGLPVDRREYGAAAAILADLGLDRVRLLTNNPAKVEGLRASGIVVTERLPHHAGQHA
                     ENETYRRTKVERMGHLLGDDA"
     gene            2363..2833
                     /gene="ribH"
                     /locus_tag="VFC06_01360"
     CDS             2363..2833
                     /gene="ribH"
                     /locus_tag="VFC06_01360"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HZL80583.1"
                     /translation="MSGHGAPNISIDGAGKTVNIVASQWHTEVMDGLVAGALRAVEAA
                     GATARVLRVPGAFELPVVAEKLARGGADVIVCLGVVVRGDTPHFDYVCEAVTRGLTDV
                     ARSHAVAVGFGVLTTDDDAQALARAGLEGSREDKGAEAVEAALATAAVLASIDA"
     gene            2913..3176
                     /locus_tag="VFC06_01365"
     CDS             2913..3176
                     /locus_tag="VFC06_01365"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HZL80584.1"
                     /translation="MTSFNELFETLSARALQRPKGSGTVTLLDKGVHAIGKKLVEEAA
                     ESWMAAEFQSDAETAEEISQLLYHAQVMMIAKGLTLADVEKYL"
     gene            3186..>3801
                     /gene="hisG"
                     /locus_tag="VFC06_01370"
     CDS             3186..>3801
                     /gene="hisG"
                     /locus_tag="VFC06_01370"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HZL80585.1"
                     /translation="MLRIAVPNKGSLSDPAVALLIEAGYKQRRDPRELVLTDSRNEVE
                     FFFLRPRDVAVYVGSGTVHAGITGRDLLLDSGAAATEILELGFGGSTFRYAAPPGEIT
                     SIQELAGKRVATSYEHLVDTHLRSFGIEADVVRLDGAVESSVRLGVADAVADVVSTGT
                     TLKAAGLEVFGEPLLKSEAILVRARSVPEADVAVLAARLRGVLMA"
BASE COUNT          652 a         1227 c         1248 g          674 t
ORIGIN      
        1 tcgcgcgcga ccagcgtggc cactccatcg acgtgcccct gcatgatgtg gcctccaagc
       61 cgcccggtag cgagcatggc gcgttcaagg ttgaccggga gccctcggtg aggtcaccca
      121 gcatcgaagt ctccagggtg atgcgcatga catcggccgc ccaggtgtcc gtgtagagcg
      181 tcgccacagt gaggcacacc ccgttgacag cgatggactc tccgtggaca agatcgtcgc
      241 cgagccccgc cgaggagatg gtcagtcgcg ctgtcgcgcc gccatcctcg atcgaggtga
      301 cggttccgac cgactgaacg atgccggtga acattacttt cccttcgtca tgtgggccgt
      361 gaccaggcaa tccacgccga gggtggtgac ggtgacgtcc tcacctctca acgcgtcggc
      421 catggaggcg attcccaggt ctgtgatcgc agcgatgccc ccgccaagca aggcgggcgc
      481 gatgtacacg ttgatttcgt cgaccaggcc cgcccgcatg aatgctgtca gaatgtgtcg
      541 gccgccttcg acaatgacgg tccgcgcctg cctggatccg aggatggcgg cgcgacggcg
      601 cgactgacca tctcctcggt ggggctcggc gacgatcttg tccacggaga gtccatcgca
      661 gtcaacgggg tgtgcctcac tgtggcgacg ctcgacaccg acacctggac cgccgatgtc
      721 atgcgcatca ccctggagac gtcgatgctg ggtgacctca acgagggctc ctcggtcaac
      781 cttgaacgcg ccatgctcgc taccgggcgg cttggaggcc acatcatgca ggggcacgtc
      841 gatggagtgg ccacgctggt cgcgcgcgac tcgcaagcgg actgggatga cctcacgttc
      901 gccttgcctt tggccctcct gcgctacgtg gtccgcaagg gttcgatcgc cctgaacggt
      961 gtttctctga cggtggcgga gcttgaagga gaccgggcga cggtttccct cattcccacc
     1021 acgctcacgg gtactacgtt cggcgactac gccgtcggcg acaaggccaa cgtggaggtc
     1081 gatgtgatcg ccaaatacgt cgagcgcatg atgggtgacc actcgtgacc gacgcgacaa
     1141 ttgcactcgt cgagcgggca ctcgctgacc tcgaggcagg caagccggtt ctcgtgtgcg
     1201 actctcacga tcgcgaggac gaagcggact tcatcatgtc cgcggagcgt gtgacggcgg
     1261 actgggtggc atggggaatc cggcactcca gcggatacct gtgtgcaccg atgcagagct
     1321 ccaaggcaga tgccctgcac ctcccgttga tggttcccca cagccaagac ccgagacgca
     1381 ccgcatacac ggtatccgtg gacgccgcga gcggtgtcac cacgggaatt tccgcggcgg
     1441 accggcacca cacgctgcaa gtcttgtccc gtgcggacgc caagcccgac gatgtcattc
     1501 gcccgggcca cgtcttgccg ctgcgcgccg ttcccggcgg agttctgcat agagctggcc
     1561 acaccgaggc cgccgtggat ctttgccgac ttgccggcct cgagccggta gcagtgatcg
     1621 cggaactcgt gaatgacgat ggcacgatga tgcggcaggg agatgccacc gcactcgccg
     1681 acaaagaggg tctgatcctc atgaccatcg cggacctcat ctactaccgc cgcgccaaga
     1741 aggacctacc cctacatatc gagcctcagc ctcatcgggt gctccttgag ggcgaggccc
     1801 gcctgcccac cgtgcacggg gagttcacgg tcaaggcata ccgggacgct cgcaccgggg
     1861 atgagcacct tgcggtctac aaggagccca ccggtgcgga agtgccgatc gtgcgggtgc
     1921 attccgaatg cctcacaggg gacgccttct cgtctttgcg ctgcgactgc ggtccacagc
     1981 tcgacgccgc gctcgttcgg gtacaggccg cgggtggagc ggtggtgtat ctcaccggac
     2041 acgaggggcg cggcatcggc atcatctcca agattcaggc ctacgcgctc caagatgccg
     2101 ggcgcgacac tgtcgacgca aaccttgact tgggcttgcc cgtagaccgt cgcgagtacg
     2161 gcgccgcagc cgcgatcttg gcggacctcg gtcttgaccg cgtgcggttg ctgaccaaca
     2221 accccgccaa ggtcgaaggg cttcgcgcct cgggcatcgt cgtcactgag cggttgcctc
     2281 accatgcggg acaacatgcc gaaaatgaga cttatcggcg cactaaggtg gaacgcatgg
     2341 gacatctgtt gggagacgac gcgtgagtgg gcacggcgcg ccaaacatca gcatcgacgg
     2401 tgccggcaag accgtcaaca tcgttgcgtc gcagtggcac acggaggtca tggacgggct
     2461 cgtcgcgggc gcactgcgtg cggtcgaggc cgccggcgcg acggcccgcg tgctccgcgt
     2521 gcccggcgcc tttgaactcc ccgtcgtggc ggagaagttg gcgcgcgggg gcgccgacgt
     2581 catcgtctgc ttgggagtcg tcgtgagggg agacacgccg cacttcgact acgtgtgcga
     2641 agcagtcacc cggggactca cggatgtggc ccgttcgcat gccgtcgcgg tcggtttcgg
     2701 cgttctcacc acggacgacg atgcgcaggc ccttgcgcgt gcgggactcg agggttcgcg
     2761 cgaggacaag ggcgctgagg ccgtcgaggc cgctctggcg accgccgccg tgttggcctc
     2821 catcgacgcc taacccggcg cacggtggtt cttgtggcgc cgtaggtgcg ccccgtcgcg
     2881 ttgagtcccg tcagtcctac taggcttgcc gcgtgacttc cttcaatgaa ctcttcgaga
     2941 ctctctccgc ccgtgcactg cagcgcccga aaggctctgg caccgtgacc ttgcttgaca
     3001 agggcgtgca cgcgatcggg aagaagctgg tggaagaagc agcagagtcc tggatggcgg
     3061 ccgaattcca gtcggacgcc gagacggcgg aagagatctc gcaattgctt tatcacgcgc
     3121 aagtgatgat gatcgccaag ggtctgaccc tcgccgacgt cgagaaatac ctgtaggggc
     3181 cagccatgtt acgtatcgcc gttcccaaca aaggatccct gtccgatccc gccgtagcgc
     3241 ttctgatcga ggccggctac aagcagcgcc gagacccccg cgagcttgtc ctcacggact
     3301 cgcgcaacga ggtcgagttc ttctttctgc ggccccgaga cgtcgccgtc tacgtgggct
     3361 ctggaaccgt tcacgcgggc atcacagggc gagacctgct actcgacagt ggcgccgcgg
     3421 cgaccgaaat tcttgagttg ggctttggcg gatcgacgtt ccgctatgcg gcgccccccg
     3481 gtgagatcac cagcattcag gagttggccg gaaagcgtgt ggccacgtcc tacgaacacc
     3541 tggtcgacac tcacttgagg tcgtttggca ttgaggcgga cgtggtgcga ctcgacggcg
     3601 ccgtggagtc ctccgtgcgc ttgggagtag ccgacgccgt ggccgacgtg gtgtcgacgg
     3661 gcaccacact caaggctgcg ggccttgagg tgttcggcga gcccctcctc aagtcggagg
     3721 ccatcctggt gcgcgccagg tccgtccccg aagccgatgt cgcggtgttg gcggcccggc
     3781 tacgaggcgt cctgatggcg c
//
LOCUS       DASRKH010000081         2100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_32666, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000081 DASRKH010000000
VERSION     DASRKH010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32666"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(330..1160)
                     /locus_tag="VFC06_01375"
     CDS             complement(330..1160)
                     /locus_tag="VFC06_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009358018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZL80586.1"
                     /translation="MSTSIEILDRLRNPNRPLRGLYSGNARAVIERGFKVILTQNWMI
                     LVSGFFEPVFYLMAMGFGLGGLIGTVTGPGGQEIEYSAYIAPALLATSAMNGAIYDST
                     WNVFFKMHFAKLYQGMMSTSLGPLDVAIGEIFMALFRGFLYALGFMAVLYALDIVNGV
                     WALLMIPAAVLVAFGFASFGMGVTSFMKTFQQMDVINFFMLPMFLLSSTLYPISVYPE
                     AIQWFVKVMPLWHGIELMRQLSVGIFTAMTPVHIGYFLVMTVLGLWLTTARLAKLFLR
                     "
     gene            complement(1157..2026)
                     /locus_tag="VFC06_01380"
     CDS             complement(1157..2026)
                     /locus_tag="VFC06_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZL80587.1"
                     /translation="MTEIAPGALTDSNRLAYTGRATPPAVQSASARRFGWWYFAEHML
                     RQMRGYGWPVLVYDVGQPLLYLFAMGVGLGAVVDANAGAVDGVPYLTFVAPALLVSTA
                     VMTAAGEMTYPVMSGFKWQRIYYGPVASPISPGQIALGHILAVSFRFVAQATVFWGLM
                     QLFGASRSGWSWLVIPIGALSALAFAAPLQAYAATLKDEGFQFSFIQRFIVMPMFLFA
                     GTFFELSSMPIYLRWIGWLSPVWHGTELARVANYGRDLAPGMTLVHLGYLLALLTVGL
                     LLANRNYRRRLTE"
BASE COUNT          410 a          679 c          733 g          278 t
ORIGIN      
        1 cctatgtcga ccttccgaca gggcactggc cgatgtggtc gaagccggtc gagctggccg
       61 aggtcatcgc gagggccgcc gggtaaacga ttcaataagc gagaccgcga cgggacaaag
      121 gtgccgcgcc ggcccacggc aagggcggca acggcgtgcg cctgcggcga gacgtcccac
      181 cggtccgagg cctcccggga gcaggcgtag cggattctgc tccgggggct cccgggaggt
      241 tcgggaccac gggatcgcgg ggcgaactgt gcgggcgggg taagccgggg gcccagccgc
      301 gggcggcggg cgccaccaag gggcgagggc taccgcagga acagcttcgc caaccgagcc
      361 gtcgtgagcc acagcccgag cacggtcatc acgaggaagt aaccgatgtg gaccggcgtc
      421 atcgcggtga agatgcccac ggagagctgc cgcatcagct cgatcccgtg ccagagcggc
      481 atcaccttca cgaaccactg gatcgcctcg gggtacaccg agatggggta gagggtggag
      541 gacagcagga acatcggcag catgaagaag ttgatgacgt ccatctgttg gaaggtcttc
      601 atgaagctgg tgacgcccat gccgaagctc gcgaacccga aggccacgag caccgcggcc
      661 gggatcatca ggagtgccca gacgccgttg acgatgtcga gggcgtacaa caccgccatg
      721 aagcccagcg cgtagaggaa gccgcggaac agcgccatga agatctcgcc gatggccacg
      781 tccagtgggc cgagcgaggt ggacatcatg ccttggtaca gcttcgcgaa gtgcatcttg
      841 aagaacacgt tccaggtgga gtcgtagatg gcgccgttca tcgcggaggt ggcgaggagg
      901 gccggggcga tgtatgccga gtactcgatc tcctgtccac ccggcccggt gaccgtgccg
      961 atcaggccac ccagcccgaa gcccatcgcc atcaggtaga acaccggctc gaaaaacccg
     1021 gagaccagga tcatccagtt ctgggtgagg atcaccttga aaccgcgctc gatcacggct
     1081 cgggcgttgc cggagtacag cccacggagc gggcggttgg ggttgcggag ccggtcgagg
     1141 atctcgatgg aggtgctcac tcggtcagcc tccggcggta gttgcggttc gcaagcagca
     1201 gccccacggt gaggagcgcc aggaggtacc cgaggtgcac cagcgtcatg cccggggcga
     1261 ggtcacgccc gtaattcgcc acccgggcga gttccgtgcc gtgccacacc ggggagagcc
     1321 acccgatcca ccggaggtag atcggcatcg aggacagctc gaagaacgtg cccgcgaaca
     1381 ggaacatcgg catcacgatg aatcgctgga tgaaggagaa ctggaatccc tcgtccttca
     1441 gcgtggcggc gtacgcctgc aacggcgccg cgaacgcgag cgccgagagc gccccgatgg
     1501 ggatcaccag ccacgaccac ccactccggc tcgccccgaa gagctgcatc agcccccaga
     1561 agacggtcgc ctgcgccacg aaccggaagc tcactgcgag gatgtgcccg agcgcgatct
     1621 gcccgggcga gatcggcgac gccaccggcc cgtagtagat ccgctgccac ttgaagccgc
     1681 tcatcaccgg gtacgtcatc tcgccggccg ccgtcatgac cgccgtcgac accagcagcg
     1741 ccggcgccac gaacgtcagg tagggcacgc cgtcgacggc cccggcgttc gcgtcgacga
     1801 cggcgcccag ccccaccccc atcgcgaaca ggtagagcag tggctggccg acgtcgtaca
     1861 cgaggaccgg ccagccgtac cccctcatct ggcgcagcat gtgctcggcg aagtaccacc
     1921 agccgaaacg gcgtgcgctc gcggactgga ccgcgggggg cgtggccctg ccggtgtagg
     1981 cgagccggtt ggagtcggtg agggcgccgg gggcgatctc agtcaacgag gctccttccg
     2041 gtcagtcgca ggaacacgtc ctccagcgag gagcgccgca cgagcgaggt gatcgggttg
//
LOCUS       DASRKH010000082         2023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_23632, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000082 DASRKH010000000
VERSION     DASRKH010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23632"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(255..581)
                     /locus_tag="VFC06_01385"
     CDS             complement(255..581)
                     /locus_tag="VFC06_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80588.1"
                     /translation="MVRRAVAERSDLSSGSIFALAMDRHASVRSSVVANPTTPGPILL
                     ALSTDTNATVRDAALAACNERICDAFAIAPSNAPAIESLREQAWWEMTPESSEVVLAK
                     VLFPDA"
     gene            complement(895..1890)
                     /locus_tag="VFC06_01390"
     CDS             complement(895..1890)
                     /locus_tag="VFC06_01390"
                     /inference="COORDINATES: protein motif:HMM:NF013938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80589.1"
                     /translation="MPAALATPKLTVVDEVLERWEQAGRHSTPPETLDALAGSDEWEI
                     RVEVAENAMTPQETLAHLADDETLEVRVAVAKNPATPPKTLDTLAWGEEDVQCAVAEN
                     PSTPPATLTSLAEVPEEALGQILADNPSTPPEVLAALAEAGDDNTRAGVGYNPSTPPE
                     TLVALAQSGDEYTRHAVAQNPSAPPETLTALAESDDEHAQYVVAGNRSTPPETLARLA
                     TGSNENAQVAAARNPSTPVETLVAIAESGNEDVQCGVAENPSLPSKTLAALAQEGDSS
                     ATQTAYRYVGKRICATFDIDKANIAAIDLLRDQAWFEMTPDSAAVVLARTLHPNA"
BASE COUNT          313 a          678 c          657 g          375 t
ORIGIN      
        1 acagcccgtc atccttgatg acgcgcaacg cgtagtcgtc agagaagccg acgaacgtcg
       61 ccaacgcctt gtcgatgttg ctcttctcgg tctgctcaac tacggggctc atggcgtctc
      121 caaccttcgg cggcggattc cccgtcgtcc ctcttgggtt acattcgtcg aaagcctacc
      181 acacagttgc gacatgggaa acgaacagcg gcgcaacgca ttgcgggcgc cccggaccaa
      241 aagtgtctag cgctctacgc gtcggggaac agcaccttgg caaggacgac ctcagaagac
      301 tcaggcgtca tctcccacca tgcctgctca cgcaacgact cgatggcggg cgcattggaa
      361 ggggcaatag cgaacgcgtc gcaaatgcgc tcattgcagg ccgcaagcgc agcgtctcgc
      421 accgtcgcat tggtatccgt ggagagcgcg agcaggatgg gtccgggcgt ggtggggttc
      481 gccaccacac tgctgcgcac gctggcgtgg cgatccatgg cgagcgcgaa gatcgacccc
      541 gaggaaaggt ccgagcgttc agcgaccgcg cgacgcacca tcggattcgc gtcgacacgc
      601 agcgtctcca ctaaggacgg tggtgttgac gggttctctg ccacacgccg tctcacaccc
      661 gcagcggagt cgcgggccaa cgcggcaagc ccgtgcgaga cggccgggtc ttctgctgct
      721 gcgcgtctcg tgcccaaatc gaccggaggc aaccagccag gccggggcag cgccgtggga
      781 ggggttgcgc cacgagtccc accgtgcact ggacagttcg ctgcgccacc ggccacgtga
      841 ccgactttcg gcgcactgca cttggggctc ataggttcac gttatccagt ccgctcacgc
      901 gttcgggtgc agcgtccggg cgagcacgac cgccgctgaa tccggcgtca tctcgaacca
      961 ggcctgatcc cgcagcagat cgatggccgc gatgttggcc ttgtcgatgt cgaaggtcgc
     1021 acagatgcgc ttaccgacgt agcggtacgc cgtctgggtg gcgctcgagt caccctcctg
     1081 ggcgagggcg gccagcgtct tggacgggag gctggggttc tctgcgaccc cgcactgcac
     1141 gtcctcgttg ccgctctcag cgatcgccac cagcgtctct actggggttg acgggtttcg
     1201 tgccgccgcg acctgggcgt tctcgttgct gcccgtcgcg agtcttgcaa gcgtctcggg
     1261 aggggtgctg cggttgcccg ccaccacgta ctgcgcatgc tcgtcgtcgc tttcggcgag
     1321 agccgtgagt gtctcgggtg gcgcgctcgg gttctgtgcc acagcgtgac gcgtgtactc
     1381 gtcgccgctt tgtgccagtg ccacgagtgt ctccggcgga gtggaggggt tgtatcccac
     1441 accggcgcga gtgttgtcgt cacccgcctc tgcgagagcg gccaacacct cgggcggcgt
     1501 ggaggggttg tcggcaagaa tctgtcccag agcctcctcc ggcacttccg cgagcgacgt
     1561 cagggtcgcg ggtggtgtgc tcggattctc cgcgaccgcg cactggacat cctcttcgcc
     1621 ccacgcaagc gtgtctaacg tcttgggagg ggtcgccggg ttcttcgcca cggccacccg
     1681 gacctccaac gtctcgtcgt ccgcgaggtg cgccagcgtc tcctgcgggg tcatcgcgtt
     1741 ctctgcgacc tcaaccctga tctcccactc atcgctcccc gcgagcgcgt ccagcgtctc
     1801 tggcggcgtg gagtgcctcc ctgcctgctc ccacctctcg agtacctcgt cgacgaccgt
     1861 gagtttcggc gtggccagtg cggcgggcaa gagaccacgt gcggcactcc gatgcacggg
     1921 gcacttctcg gcagacccaa acttccgatg gccctcgaca ggtgcgctgc atttgctcat
     1981 ggccggacgg tatccacggc caggctcact ggttcgggaa gag
//
LOCUS       DASRKH010000083         2030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_4697, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000083 DASRKH010000000
VERSION     DASRKH010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4697"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VFC06_01395"
     CDS             complement(<1..525)
                     /locus_tag="VFC06_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80590.1"
                     /translation="MREITDRAPARAIHAGPAIAHTPRRRGPRPGRVVLYAGVVIGAV
                     AMLFPFLWTVITSITPNGTLAAGPTLVVDNPTLEAYTTLFTSMPMWRIVANSFGIAIA
                     STVLQMITGSMAAYAFGRMEFRGKNVLFGLYLATLMVPLQVLVVPLFIEMTKLHVQDT
                     YFALLAPSIAAAFGI"
     gene            complement(518..1480)
                     /locus_tag="VFC06_01400"
     CDS             complement(518..1480)
                     /locus_tag="VFC06_01400"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HZL80591.1"
                     /translation="MPTSAVLAAPSAVTPTADVARRTSASRSGRTRGWKNTAWVLFFL
                     LPSAVPLVAFTLVPMITSAWISLHKWNLISAMQFVGLDNYSKLLSDPGTRRVFGHTLL
                     YIAGYLPLVYVGGLALAMALNQAFRGRSFFRAAYFLPVVTSWVVVALVWQWLLNPKNG
                     LVNHVLGVFHLPQPGWWTDPSWAMPSVILASAWKDLGFVMVILLAGLQSIPADIQEAA
                     RIDGANAWQRFRRITLPLLSPSTFFVLVISLINGFQVFDQVYVMTGGGPAGSSQVVVG
                     QIYDLTFRYGRAGEASALSWLLFILILAITAIQLRGQKKWVTHA"
     gene            complement(1495..>2030)
                     /locus_tag="VFC06_01405"
     CDS             complement(1495..>2030)
                     /locus_tag="VFC06_01405"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HZL80592.1"
                     /translation="AGEIGHIPMSVDGPLCGCGSRGCLEAHIGDDALVRQALEAGVIG
                     PRGSLDGLKIAAGRGDAVAQGIYREAGAVLGRALAGVVHTVDPEVVVLLGEGIDAWPH
                     WEAGFEQSLRSHLMPARRGLPFIVEPWAEDKWALGAAALVLATPFDAAGAAGDQGQLV
                     RDRLQAGGPAIAAGATL"
BASE COUNT          353 a          682 c          724 g          271 t
ORIGIN      
        1 gatgccgaat gcggcggcga tcgacggcgc gagcagggcg aagtacgtgt cctggacgtg
       61 cagcttggtc atctcgatga acagcgggac gaccagcacc tgcaacggga ccatcagggt
      121 cgccaggtac agaccgaaga gcacgttctt gccgcggaac tccatgcgcc cgaaggcgta
      181 ggccgccatc gacccggtga tcatctggag cacggtcgac gcgatcgcga tcccgaacga
      241 gttcgccacg atccgccaca tgggcatcga cgtgaacagg gtcgtgtacg cctcgagagt
      301 ggggttgtcg acgacgaggg tcggcccggc agcgagcgtc ccgttgggcg tgatcgaggt
      361 gatgacggtc cacaggaacg ggaagagcat cgcgaccgca ccgatgacga cgccggcgta
      421 caggacgacc cggcccggcc gcgggccccg ccgccgtgga gtgtgggcga tcgctgggcc
      481 cgcgtggatc gcacgggcag gcgctcggtc cgtgatctca cgcatgggtc acccacttct
      541 tctgcccgcg cagctggatc gcggtgatgg ccaagatgag gatgaacagg agccaggaga
      601 gcgcggaggc ctcaccggcg cgcccgtagc ggaacgtcag gtcgtagatc tggccgacga
      661 cgacctggct cgatcccgca gggccaccgc ccgtcatgac gtagacctgg tcgaacacct
      721 ggaagccgtt gatcagcgag atcaccagga cgaagaacgt cgagggcgac agcagcggaa
      781 gggtgatccg gcggaagcgt tgccaggcgt tggctccgtc gatccgcgcg gcctcctgga
      841 tgtccgcggg gatcgactgc aggccggcga gcaggatgac catgacgaag cccaggtcct
      901 tccaggctga ggcgaggatg accgagggca tcgcccagga cgggtctgtc caccagcccg
      961 gctgcgggag gtggaagacc cccagcacgt ggttcacgag cccgttcttg gggttcagca
     1021 gccactgcca caccagggcg accacgaccc agctcgtcac caccggcagg aagtacgccg
     1081 cccggaagaa cgagcggcca cgaaatgcct ggttcagcgc catcgcgagc gcgaggcccc
     1141 cgacgtagac cagcggcagg tatccggcga tgtacagcag ggtgtggccg aagacccgcc
     1201 tcgtgcccgg atcgctcagg agcttgctgt agttgtccag cccgacgaac tgcatcgcgg
     1261 agatcaagtt ccacttgtgc aggctgatcc acgcggacgt gatcatcggg accaacgtga
     1321 aggccacgag gggtaccgcg ctcggcagca ggaagaagag cacccacgcg gtgttcttcc
     1381 atcctcgagt ccgacccgag cgcgacgcgc tcgtgcggcg cgccacgtcg gctgtcgggg
     1441 tgacggcgct cggcgcggcg aggaccgctg aggtcggcac agccttcggg gccgtcacag
     1501 ggtcgctccg gcggcgatcg cgggaccgcc ggcctgcagc cggtcgcgca cgagctggcc
     1561 ctggtccccg gcagcgccgg cggcgtcgaa cggcgtggcg agcacgagtg ctgcggcacc
     1621 cagtgcccac ttgtcctcgg cccacggctc gacgatgaac ggcaggccac gccgggcggg
     1681 catcaggtgg ctgcgcagcg actgctcgaa gccggcttcc cagtgcggcc acgcgtcgat
     1741 gccctcaccg aggagcacga cgacctccgg gtcgacggtg tgcacgaccc cggccagcgc
     1801 ccgaccgagg accgctccgg cctcgcggta gatcccttgc gcgacggcgt ccccgcggcc
     1861 tgcggcgatc ttgagcccgt cgagcgagcc gcgtggtccg atcacgccgg cttcgagggc
     1921 ctggcgcacc aaggcgtcgt cgccgatgtg ggcctccagg caaccgcgcg agccgcagcc
     1981 gcacagtggt ccgtcgacgc tcatggggat gtggccgatc tcgcccgcgc
//
LOCUS       DASRKH010000084         2281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_41540, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000084 DASRKH010000000
VERSION     DASRKH010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41540"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VFC06_01410"
     CDS             complement(<1..527)
                     /locus_tag="VFC06_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HZL80593.1"
                     /translation="MSHPHPELSLPARLRDGTLRITPLGGLGEVGRNMSVLELNRKLL
                     IVDCGVLFPEDNHPGVDLILPDFTSIRDRLDDVVGLILTHGHEDHIGAVPYLLRQRGD
                     IPIIGSELTLAFIEAKLKEHRITPYTMRVAEGQIEDFGPFECEFVAVNHSIPDGLAVM
                     IRTVAGNVLHTGDFK"
     gene            complement(561..1451)
                     /gene="dapA"
                     /locus_tag="VFC06_01415"
     CDS             complement(561..1451)
                     /gene="dapA"
                     /locus_tag="VFC06_01415"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HZL80594.1"
                     /translation="MDARTAFGTVIPAMVTPFHADGSLDLASAQRLADHLVTEGCDGI
                     VVSGTTGESPTTHGPEKADLVRAVVEAVGGRARVIAGAGSNDTAHAVCMAEAAAQAGA
                     QALLVVSPYYNRPSQDGVAAHIETIASATDLPVMVYDIPGRTGVAIGPECSARIARHP
                     NVVAFKDATGNPVAGCRRGRETGMAVFSGDDALNLTFLTHGAVGVVSVVAHAGARQYR
                     RMVDAVAASDLAAALDAHNAVMPLVDAIMGAGQGAVLAKLAAFEQGLISTPTLRLPLV
                     PATAAQREALLRAMEAVRAL"
     gene            1528..2073
                     /locus_tag="VFC06_01420"
     CDS             1528..2073
                     /locus_tag="VFC06_01420"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZL80595.1"
                     /translation="MADVSVRPALPADSERIGAIHAETLRLAAEAGLGRDLPDEAADG
                     LGPAAFAAQWRAAIDAPPTPLHRVLTALDGQVIVGFAALAPADGDLPHAGAALQAEIV
                     ALEVAPDRVRAGHGSRLLAACAEILRQQNAVAVLTWCVQGDQARTRFLTSAGFAPLGV
                     RRRFAVGDGEITQIAWHTTLG"
     gene            complement(2084..>2281)
                     /locus_tag="VFC06_01425"
     CDS             complement(2084..>2281)
                     /locus_tag="VFC06_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017198017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HZL80596.1"
                     /translation="LLPRVAEHDLLVRHEEVVFGNPGEQLTIRTDSFDRSSFMPGVLL
                     AVRRVPGIPGLTVGLDALIEL"
BASE COUNT          312 a          786 c          870 g          313 t
ORIGIN      
        1 atcttgaagt cgccggtgtg gagcacgttg ccggcaacgg tgcggatcat caccgcgagg
       61 ccgtccggga tcgagtggtt gacagccacg aactcgcact cgaaggggcc gaagtcctcg
      121 atctggccct ccgccacccg catcgtgtaa ggggtgatgc ggtgctcctt gagcttcgcc
      181 tcgatgaacg ccagcgtcag ctccgagccg atgatcggga tgtcgccccg ctgccgcagc
      241 aggtagggca ccgcgccgat gtggtcctcg tgcccgtgcg tgaggatgag gccgacgacg
      301 tcgtcgagcc ggtcgcggat cgaggtgaag tcggggagga tcaggtccac gccggggtgg
      361 ttgtcctccg ggaagaggac cccacagtcg acgatcagca gcttgcggtt gagctcgagg
      421 acgctcatgt tgcggccgac ctcgccgagg ccaccgagcg gcgtgatgcg cagggttccg
      481 tctcgcaggc gggcggggag cgaaagttca ggatggggat gactcacggg tccatcctgc
      541 cattgaccgg agcggtgctc ctacagtgcc cgcacggcct ccatcgcgcg cagcagcgcc
      601 tcgcgctggg cggcggttgc cgggacgagc gggagccgca gggtgggcgt ggagatcagc
      661 ccctgctcga aggccgccag cttggcgagc acggcaccct gccccgcgcc catgatggcg
      721 tccaccagcg gcatgaccgc gttgtgggcg tccagcgcgg cggccagatc ggaggccgcg
      781 acggcgtcga ccatgcggcg gtactggcgc gctcccgcgt gcgcgacgac ggacaccact
      841 cccacggcac cgtgcgtgag gaaggtgagg ttcagggcat cgtcgccgga gaacacggcc
      901 ataccggtct cgcggccgcg gcggcagccg gccaccggat tgcccgtggc gtccttgaac
      961 gcgaccacgt tcgggtggcg cgcgatgcgg gcggagcact cgggcccgat cgccacgccg
     1021 gtgcggccgg ggatgtcgta caccatgacc ggcaggtcgg tggccgatgc gatcgtctcg
     1081 atgtgcgcgg ccacaccgtc ctgcgagggg cggttgtagt agggggagac caccaggagg
     1141 gcctgcgcgc cggcctgggc ggccgcctcg gccatgcaaa cggcatgcgc ggtgtcgttc
     1201 gagcccgcgc cggcgattac ccgcgcccgg ccgccgaccg cctcgacgac ggcgcgcacc
     1261 aggtccgcct tctcggggcc gtgcgtggtg ggcgactcgc ccgtggtgcc ggagacgacg
     1321 atgccgtcgc agccctcggt gaccaagtgg tccgcgagcc gctgggcgga cgccaggtcc
     1381 agcgacccgt ccgcgtggaa gggcgtgacc atggcgggga tgacggtccc gaaggcggtg
     1441 cgtgcgtcca tgcggcccac ggtagtgcct ccgggtggcg gacggggccg ccgggccggt
     1501 gggtgggaca ccggttaggg tggcggggtg gctgatgttt ccgtgcgacc cgccctgccc
     1561 gccgactccg agcggatcgg cgcgatccat gccgagactc ttcggctggc cgcggaggcc
     1621 gggctcggcc gagatctccc ggacgaggcc gcggacggcc tcgggcccgc ggccttcgcc
     1681 gcgcagtggc gcgccgcgat cgacgccccg cccacacccc tccaccgcgt gctcaccgct
     1741 ctggacggcc aggtgatcgt gggattcgcc gcgctcgccc cggcggacgg ggacctgccc
     1801 catgccggcg ccgccctaca ggcggagatc gtggcgctgg aggtggcgcc cgaccgggtc
     1861 cgcgccggcc acggctcccg cctgctggcc gcgtgcgcgg aaatcctgcg ccagcagaac
     1921 gccgtcgccg tgctgacgtg gtgcgtccag ggcgaccagg cacgcacgcg gttcctcacc
     1981 tcggccgggt tcgcgccgct gggggtgcgc cgccggttcg cggtcggcga cggggagatc
     2041 acccagatcg cgtggcacac cacgctgggc tgagccgccg ggtctacagc tcgatcaggg
     2101 cgtccagccc gaccgtgagg cccgggatcc ccggcacgcg ccggacggcg agcaggacac
     2161 ccggcatgaa actggagcgg tcgaacgagt ccgtgcggat ggtgagctgc tcccccgggt
     2221 tgccgaacac gacctcctcg tggcgcacga ggaggtcgtg ttcggcaacc cgggggagca
     2281 g
//
LOCUS       DASRKH010000085         2103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_31952, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000085 DASRKH010000000
VERSION     DASRKH010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31952"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(339..1748)
                     /locus_tag="VFC06_01430"
     CDS             complement(339..1748)
                     /locus_tag="VFC06_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HZL80597.1"
                     /translation="MTKTTGSYPRLTVDTTTSGAVGQAGGVLLTETIAVSGLGRELSA
                     ALARWRKPLAIHDPAKVITDLAVTLALGGDCMADIALLRAEPGLYGQVASDATVSRTI
                     DALAADAPAALKAIDTARAAARKRAWALAGTDAPDHDASAKSPLIVDLDATLVASHSE
                     KQNAAPTFKRGFGFHPLLSFIDHGTDGTGEPLAVMLRPGNAGSNTAADHITVIKGALA
                     QLPGHRPGTRAGRKVLIRIDGAGSTHAVLDWMTAQRLSYSVGFSLPANISDLLEHIPD
                     DVWAPAYDAHDEIRDGAWVAELTDLLKLSGWPKGMRVIVRKERPHPGAQLTITDIDGH
                     RITAFATNTRTGGPDPQLADLEIRHRRRARCEDRIRIAKDTGLRAFPLFDFTQNQIWC
                     AIVALAIEITAWMQMLALTNHPARRWEPKRLRLRLFTIPATLARTGRRVLLHLAAKAP
                     WAELAGQGITRLRALAAPG"
BASE COUNT          303 a          627 c          783 g          390 t
ORIGIN      
        1 agtccgttgg ttacgtgagc acgacggcat tgatttcgtt gaaagcgcgc gtgtcgcaag
       61 tagataccgt ggtattcaag cgggagtgga gtatgccgag ggttttcgtc cgtgcctcaa
      121 gtctcttcgt cctcggacca ttcgtttgct cccctagcct cgatctttca tggggtgccg
      181 gtgcttggtc gtggcggcgt cggggccgtc gtgtcggggt gattctggca tctgggtgtg
      241 acggtgaccc gagtgtcatc tcgggtgggc gccggttcca gggatcgggg atcgtgcggg
      301 ggttgtcggg tcgggacgcg gcggtcagcc gggggcggtc agccgggggc ggccagggct
      361 cgtagtcgtg tgattccttg tccggcgagt tcggcccagg gggctttggc ggccaggtgc
      421 agcaagacgc ggcggccggt gcgggcgagg gtggccggga tggtgaacag gcgcagccgt
      481 agccgtttgg gttcccagcg ccgtgctggg tggttggtca gtgcgagcat ctgcatccag
      541 gcggtgatct cgatggccag ggcgacgatg gcgcaccaga tctggttctg ggtgaagtcg
      601 aacagcggga aggcgcgtag tccggtgtct ttggcgatgc ggatgcggtc ttcacagcgg
      661 gcgcggcggc ggtggcggat ctcgaggtcg gcgagctggg ggtcgggtcc gccggtgcgg
      721 gtgttcgtgg cgaacgcggt gatgcggtgg ccgtcgatgt cggtgatcgt cagttgcgcg
      781 ccggggtggg ggcgttcctt gcggacgatc acccgcatcc ccttgggcca cccagacaac
      841 ttcagcaggt cggtgagctc ggcgacccac gcgccgtcac ggatctcatc gtgcgcgtca
      901 taggccggcg cccacacgtc gtcggggatg tgttcgagca ggtcagagat gttggctggc
      961 agggagaacc ctacggagta ggacaaccgc tgggcggtca tccagtccag gaccgcgtgg
     1021 gtcgagcccg caccgtcgat gcggatcagg accttgcggc cagctctggt cccgggccgg
     1081 tggcctggga gttgggccag ggcgcccttg atgacggtga tgtggtcggc cgcggtgttc
     1141 gacccggcgt tgcccgggcg gagcatcacc gccaacggtt ccccggtccc atcagtgccg
     1201 tggtcgatga acgagagcaa cgggtggaag ccaaaccctc gcttgaaggt cggggcagcg
     1261 ttctgcttct cggagtggga agcgaccagg gtggcgtcca gatccacgat caggggactc
     1321 ttcgcactcg cgtcgtggtc gggggcatcg gtgccggcca gcgcccatgc gcgtttgcgg
     1381 gctgccgcgc gggcggtgtc gatggccttc aacgcggccg gggcgtccgc ggcgagggcg
     1441 tcgatggtcc gcgagaccgt cgcgtcggac gcgacctgcc cgtagaggcc gggttcggcg
     1501 cgcagcaagg cgatgtccgc catgcagtcc ccgcccaggg cgagagtgac cgcgagatca
     1561 gtgatgacct tcgccgggtc gtgaatcgcg agcggcttgc gccaccgggc caacgccgcc
     1621 gacaactccc gcccgagccc actcacggcg atcgtctcgg tcagtagcac cccaccggcc
     1681 tgaccgaccg ctcccgacgt ggtcgtgtcg acggtgagac gtggatagga cccggtagtc
     1741 ttcgtcacct ggaaggtgct cctcgaactg gtctgatgtg gccttcaaca atccacattc
     1801 tcccagttca ggggcacttt tcagttcacc gacacgacct cacttcacca cgccatgaaa
     1861 gcgcgaggct aggtgatgcc gagcacgaca accgatccga ccgagcacgg ccagcccgtc
     1921 agaactcagg cgggccggtc agcgtgcgcg cctgcacccg cgaggaagca cgagactgag
     1981 cccttgccct gccgtagctg agatctggcc cgcctgagtt ctgacgggct ggccgtgctc
     2041 ggtcggatcg gttgtcgtgc tcggcatcac ctagcctcgc gctttcatgg cgtggtgaag
     2101 tga
//
LOCUS       DASRKH010000086         2571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_32479, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000086 DASRKH010000000
VERSION     DASRKH010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32479"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="VFC06_01435"
     CDS             complement(<1..729)
                     /locus_tag="VFC06_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4081 domain-containing protein"
                     /protein_id="HZL80598.1"
                     /translation="MVHNPFMIGRARESTLDGRPAGGRVLADVDVESALAVCALDPVA
                     SVLATARIEAAARDGLRRSGGQLWGYEDDGELVAVCWSGVNLVPVVPGRDPAALDAFA
                     ALGREHGRRSSSIVGADWAVLGLWDRLSPIWPKAREVRADQPSYVIDHDPLIEPDPLV
                     RLSVPDEYHLILPACVRMFVEEVGYSPVSGFDGPYEVRVRSLIDQHRSYVRIDEGEGP
                     REGTPAVVFKAELGAIAGGVAQVQG"
     gene            complement(815..1129)
                     /locus_tag="VFC06_01440"
                     /pseudo
     CDS             complement(815..1129)
                     /locus_tag="VFC06_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
     gene            complement(1132..1800)
                     /locus_tag="VFC06_01445"
                     /pseudo
     CDS             complement(1132..1800)
                     /locus_tag="VFC06_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
     gene            1910..>2571
                     /gene="proS"
                     /locus_tag="VFC06_01450"
     CDS             1910..>2571
                     /gene="proS"
                     /locus_tag="VFC06_01450"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HZL80599.1"
                     /translation="MLLRLSTLFVRTLREDPVDAEVASHKLLVRAGYIRRAAPGIYTW
                     LPLGLRVLAKVELVVREEMAEAGAQEVHFPALLPREPYETTGRWTEYGPNIFRLKDRK
                     GGDYLLAPTHEEVFTLLVKDLYSSYKDLPLALYQIQTKYRDEARPRAGLIRGREFIMK
                     DAYSFDVDDDGLAASYQRQRDAYERVFTRLGLEYVIVAATSGAMGGSRSEEFLTPTPI
                     GE"
BASE COUNT          370 a          950 c          924 g          327 t
ORIGIN      
        1 gccctggacc tgcgcgaccc cgccggcgat cgctcccagc tcggccttga agaccacggc
       61 gggtgtcccc tcgcgcgggc cctccccctc gtcgatccgc acgtacgacc ggtgctggtc
      121 gatgagcgag cgcacgcgca cctcgtacgg gccgtcgaat cccgagaccg gcgagtagcc
      181 gacctcctcg acgaacatgc gcacgcacgc cgggaggatc aggtggtact cgtccggcac
      241 cgagagccgt acgagggggt ccggctcgat cagaggatcg tggtcgatga cgtacgaagg
      301 ctggtcggcg cgcacctcgc gtgccttcgg ccagatcggt gagagccggt cccacaggcc
      361 gaggactgcc cagtccgccc ccacgatcga cgacgagcgt cggccgtgct cgcggccgag
      421 cgcggcgaag gcgtcgagcg cggcggggtc gcggcccggg acgacgggca ccaggttgac
      481 gccggaccag cagaccgcga cgagctcgcc gtcgtcctcg tagccccaga gctgtccgcc
      541 cgagcggcgc aggccgtccc gtgctgcggc ctcgatccgc gcggtcgcga gcaccgaggc
      601 gaccgggtcg agggcgcaca ccgcgagcgc cgactcgacg tccacatcgg cgagaacacg
      661 gcctcccgcg ggtcgaccgt cgagcgtcga ctcgcgcgca cgtccgatca tgaagggatt
      721 gtgcaccacg gacccaccgg tcggcggcgg gcgacgcgcc accgagtccc caccgcgtgc
      781 agcgaggacc cggtgacggc ggtgtcgcgc ggtgtcagcc gacggtgacg accggtgtcc
      841 gcggcgcacc gtcgccgacg ggttccatgg actcggcgag ccgcagggcc tcctcgatca
      901 gcgtctcgac gatctgcgac tccggcacgg tcttgatgac ctcgccgcgg acgaagatct
      961 ggcccttgcc gttgcccgac gccacgccga ggtcggcctc gcgcgcctca ccggggccgt
     1021 tgacgacgca gcccatgacg gcgacgcgca ggggcacgtc gagtccctcc agccccgcgg
     1081 tcacgcgttc ggcgagcgag tacacgtcga cctgcgcgcg cccgcacgac cgaccgcgtc
     1141 gcgcagcacg cccacgaccg cgcggtgcag ctcggcggta cgcgccctgg agaacgtggt
     1201 cgtcggggcg aacgggctca ggcgcgccgc gtgcaggatc tcgtcggagt aggcgttgcc
     1261 gatcccggcg atggtgccct ggtcccgcag catgcccttg acctgctgac ggcgggccgc
     1321 caggagctca cccagccggt cgaccgtgaa gtccaccgac agcggctcga cgccgagcgc
     1381 cgcgatcgcg ggcacctggt gcgggtcggt cacgacgtgg accgcgagcc gtttgcgggt
     1441 cccggcctcc gtgagcgaga agcccgagcc gtcgtcgaag cgcacgcgca gggccagcgg
     1501 agaccggccg ggccgcacgg gggccgccgg cacgtgctcg tgccagcgca gccacccggc
     1561 tcgcgcgagg tggacgacga ggtgcagcgg ctcgccgccg gtgtccggag ctgtcgacag
     1621 gtcgagccac ttgccgtgcc gggagacacc gaccacgcgt cggccgatga gctccgtcgg
     1681 cgccggggcg aacgtcttga gggcgctgat cgcgccgacc tcgacgccgg tcagcacgcg
     1741 gccgaccgtc cgctcgcgca ggaacagcgc gagcgcctcg acctcgggca gctcgggcac
     1801 gggtcccatc gtggcgcacc ggaccggccg tgcgcacccg gtgcgtgccg gtccgggctc
     1861 gcggggccgg gcgcagtgac cctccggcac cggcactagg ctcggcccca tgcttcttcg
     1921 tctgtccacg ctcttcgtcc gtaccctgcg agaggacccg gtcgacgccg aggtcgcgag
     1981 ccacaagctg ctcgtgcgcg ccggctacat ccgccgtgcc gccccgggga tctacacgtg
     2041 gctcccgctc ggcctcaggg tgctcgcgaa ggtcgagctg gtcgtccgcg aggagatggc
     2101 cgaggccggt gcgcaggagg tgcacttccc ggcgctcctg ccgcgcgagc cctacgagac
     2161 gacgggccgg tggaccgagt acggccccaa catcttccgg ctcaaggacc gcaagggcgg
     2221 tgactacctg ctcgcgccga cgcacgagga ggtgttcacg ctgctcgtca aggacctgta
     2281 ctcgtcgtac aaggacctgc ccctcgcgct gtaccagatc cagacgaagt accgcgacga
     2341 ggcacgcccg cgcgcagggc tgatccgcgg ccgcgagttc atcatgaagg acgcctactc
     2401 gttcgacgtc gacgacgacg ggctcgcggc gtcctaccag cgccagcggg acgcctacga
     2461 gcgggtcttc acgcggctcg gcctcgagta cgtgatcgtg gcggcgacgt cgggtgcgat
     2521 gggcggctcg cgcagcgagg agttcctcac cccgaccccg atcggcgagg a
//
LOCUS       DASRKH010000087         3229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_27717, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000087 DASRKH010000000
VERSION     DASRKH010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27717"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VFC06_01455"
     CDS             <1..236
                     /locus_tag="VFC06_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80600.1"
                     /translation="TLLAELRGYYAANEGWVGARVARYVFPTWAVGRIPTLESDIEEW
                     LASNADAPSVLVKIVREGLDDVRRSLAAQAASA"
     gene            complement(266..2407)
                     /gene="malQ"
                     /locus_tag="VFC06_01460"
     CDS             complement(266..2407)
                     /gene="malQ"
                     /locus_tag="VFC06_01460"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HZL80601.1"
                     /translation="MRTVNDEVHDERPSEALATLARACGVATDVHLIDGTHQTATRSA
                     LVAILDAMGIDAHDEGACHASFEELKDYVWTRIVPPVTVIREGKSREIPVHLPHGATV
                     TVKLRTEDGVERPLEQVDKVVEPHSIGSIAVGRATFRIPNDLPLGWHVIEAVFPGRRG
                     RGYVVVTPERLEVPAAMAARRPWGLMTQLYSLRSKRSWGIGDLGDLADLCSLAKIRAG
                     ADFVLINPLHANEAVPPLSPSPYLPSSRRFIAPMYIRVEDIPEVAYVPSQQRAVIEWE
                     SERPSRNNNTDELLDRDEVWRAKVTALEHVFEAPRSPGREAQFEAYCVREGKVLEEYA
                     TWAAISEEYQDGTWPEELATSSAAAVAAWRDEHADRVLFHAWMQWIADEQSAHAQSTA
                     RNAGMSIGVITDLAVGVHPSGADAWSLHRVLAEGMSVGAPPDGFNPQGQDWSQPPWQP
                     RALEASAYVAFRDVVRAAVRHSGAVRVDHILGLFRQWWIPLGHGAGDGSYVQFDHEAL
                     VGILILEAHRAGAIVIGEDLGTVEPWVREYLSSRGVLGTSVLWFERTHDGGFTPPEHY
                     RADALATVTTHDLPTTAMMLAGTQVELRAELGLIDDPVAAAAQAADELSKLVALLRER
                     NWVIEDYEEEDLIAGVHRMVLASPAVLTGVAITDAVGDMRAQNVPGTFEEYPNWRMPL
                     TDRNGVPVLLDDLFEHPRMRRLIGAIRAARG"
     gene            2465..>3229
                     /locus_tag="VFC06_01465"
     CDS             2465..>3229
                     /locus_tag="VFC06_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HZL80602.1"
                     /translation="MHAFINPHLLTASTTDPSSSGGAPWVTWVSSAMPDAPAWVQDLT
                     ASSIAVGFLLMGALIVWIIGRLMIRAVTRSVETGLPFRRRTRQALERARVELPPLDPQ
                     EVRLEAERRLQRAGTIRTVLNSALAVVLLAVLVVMILDQAGLPVAPLLASAGIVGVAF
                     GFGAQSLVKDLLSGLFMLIEDQYGVGDVVDLGPASGLVEEVGLRSTRLRSLDGTVWYV
                     PNGEIARVGNMTRLWSRAFIEVRLSYDTDLDEARDHS"
BASE COUNT          512 a         1090 c         1066 g          561 t
ORIGIN      
        1 ctacactgct ggccgagctg cgcggctact acgccgccaa cgagggctgg gtgggcgcgc
       61 gagtggcccg ctacgtgttc cccacgtggg ccgtcggccg gatccccacc ctcgagtcgg
      121 acatcgagga gtggttggca agtaacgcgg acgccccctc ggtattggtc aagatcgtgc
      181 gtgagggtct cgatgacgtg cggcgatcgc tggccgcaca ggccgcgagc gcgtaaggct
      241 cgcgcctcgg cggtcactcg gacctttagc cgcgggccgc gcgtatagcc ccgatgagtc
      301 ggcgcatgcg cgggtgctcg aacagatcgt caagcaacac cggcaccccg ttgcggtcag
      361 tgagcggcat ccgccagttg gggtactcct cgaaggtgcc cggcacgttc tgcgcgcgca
      421 tgtcgcccac ggcgtctgtg atggcgacgc ccgtgaggac ggcaggcgag gcaagcacca
      481 tccgatgcac ccccgcgatc aggtcttcct cttcgtagtc ctcgatcacc cagttgcgct
      541 ctcgaagcag ggccacgagc ttgctcaact catcggccgc ctgcgccgcc gcagccaccg
      601 ggtcgtcgat caggcccagt tccgcgcgca actccacctg cgtgccagcg agcatcatgg
      661 cggtcgtcgg aaggtcgtga gtggtgacgg ttgccagggc atcggcgcgg tagtgctcgg
      721 gcggcgtgaa gccaccgtcg tgcgtgcgct cgaaccacag caccgacgtg ccgagcacgc
      781 cgcgcgagct caggtactcg cgcacccacg gttccacggt gccgaggtcc tcgccgatga
      841 cgatggctcc tgcacgatgc gcttccaaaa tcagaatgcc gacgagagcc tcgtggtcga
      901 actgcacgta ggagccgtcg cctgcgccgt gacccaacgg gatccaccac tgccggaaga
      961 gccccaagat gtggtcgacg cgcactgccc ccgaatgtcg cacggcggca cggaccacgt
     1021 cgcggaacgc cacatatgcg gaggcctcga gtgctcgcgg ctgccagggc ggctgcgacc
     1081 agtcctgacc ttgagggttg aagccatcgg gcggcgcacc cacgctcatg ccttcggcga
     1141 gcaccctgtg aaggctccag gcatcggccc cggacgggtg gacgcccacg gcgaggtcgg
     1201 tgatgacgcc gatgctcatg cccgcgttgc gggccgtcga ctgcgcgtgc gcggactgct
     1261 cgtcggcaat ccactgcatc caggcgtgga acaacacccg gtcggcgtgt tcgtcccgcc
     1321 atgcggcaac ggccgcggcc gatgaagtcg cgagctcttc gggccaggtc ccatcctggt
     1381 attcctccga gattgctgcc cacgtggcgt attcctcgag caccttgccc tcgcgcacgc
     1441 aatacgcttc aaactgcgct tccctgccag gggaacgcgg cgcctcgaag acgtgctcga
     1501 gcgccgtcac cttggcgcgc cacacctcgt cgcgatccag gagctcgtcg gtgttgttgt
     1561 tgcggctggg gcgctccgac tcccactcga tgaccgcacg ctgttgcgac ggcacgtacg
     1621 ccacctcggg gatgtcctcg acgcggatgt acatgggggc gatgaatcgt cgcgacgacg
     1681 gcaggtatgg ggaggggctc agcggaggca ctgcctcgtt ggcgtgcagg gggttgatga
     1741 gcacaaagtc cgctccggcg cggatcttgg ccagggagca caggtcggcg aggtcaccaa
     1801 ggtcgccgat gccccaggag cgcttggacc gcagcgaata cagctgagtc atgaggcccc
     1861 aaggacggcg ggcggccatc gccgcaggca cctcaaggcg ttcgggcgtc acgaccacgt
     1921 agccgcgacc gcgtcgccca gggaacaccg cctcgatcac gtgccagccg aggggcagat
     1981 cattgggaat gcggaacgtc gcacgaccca ccgcaatcga gccgatgctg tgcggctcca
     2041 ccaccttgtc gacctgttcg agcgggcgct cgacgccgtc ctccgtgcgt agcttcacgg
     2101 tcaccgtcgc gccatgcggc aggtgcaccg gaatctcgcg cgacttgccc tcgcgaatca
     2161 ccgtgaccgg cggcacgatg cgcgtccaca cgtagtcctt gagttcctcg aacgaggcgt
     2221 ggcaggcacc ttcgtcatgc gcgtcaatgc ccatcgcatc caagattgcc accaaggcgc
     2281 ttcgcgttgc ggtctgatgc gttccgtcta tcaggtggac atcggtcgcc accccgcagg
     2341 cgcgcgcaag ggtcgccagc gcctcggacg gcctctcgtc gtgcacttcg tcgttcacgg
     2401 tcctcatcct gccaggttta gcacgcattt cgggaggcct cgccggggcc attagtctgg
     2461 aacgatgcac gccttcatca atcctcacct gctgaccgcc tccacaacag atccctcgtc
     2521 cagcggtggc gcgccatggg tgacgtgggt cagttccgcc atgcccgacg cccccgcgtg
     2581 ggtgcaggac ctgaccgcat cgtcaattgc ggtcgggttc cttctgatgg gcgccctgat
     2641 cgtgtggatc atcggcaggc tgatgatccg cgcggtcact cgtagcgtcg agacggggct
     2701 gccgttccgg cgccgaaccc gtcaagcact tgagcgagct cgcgtggaac ttccgcccct
     2761 cgatccccaa gaggtgcgcc tggaagcgga acgccgactc cagcgcgcgg gcaccattcg
     2821 cacggtgctg aattccgcct tggcagtcgt tcttcttgcc gtgctggtgg tgatgatcct
     2881 tgatcaggcg gggctgcccg tcgcgccgct cctcgcgtcg gctggaatcg tcggcgtggc
     2941 cttcggcttt ggcgcccaat cgctcgtgaa ggacctgcta tccggcctgt tcatgctgat
     3001 cgaggatcag tacggcgtgg gcgatgtcgt ggatcttgga ccggcaagcg gtctcgtgga
     3061 agaggtcggg ctgcgttcca cgcggctgcg atccctcgat ggcacggtct ggtacgtgcc
     3121 caacggtgaa attgcgcgcg tgggcaacat gacgcgactg tggtcgcgcg ccttcatcga
     3181 agtgcgtttg agctatgaca cggatctcga cgaggcgcgc gaccacagt
//
LOCUS       DASRKH010000088         2211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_4682, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000088 DASRKH010000000
VERSION     DASRKH010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4682"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VFC06_01470"
     CDS             complement(<1..301)
                     /locus_tag="VFC06_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HZL80603.1"
                     /translation="MFSNAEEAIAYTREEAVEFIDVRFCDLPGVMQHFNIPVQAFTDD
                     VFTEGLMFDGSSIRGFQAIHESDMKLLADISTAYLDPFRAEKTLNVNMSIVDPYTD"
     gene            complement(429..1136)
                     /locus_tag="VFC06_01475"
     CDS             complement(429..1136)
                     /locus_tag="VFC06_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4191 domain-containing protein"
                     /protein_id="HZL80604.1"
                     /translation="MSNSAPAPTPKPKKRRWYNNFIDAYRLTAGAEPWVGWAMAGGLL
                     ASAGIGVGIGYAVGHPIYVGFLGAMAGLTVAMFILSWRVKKVSYAQIEGQPGASIAVL
                     DQIKRGWNIEQEPVAVNPRTQDLVFRMVGRPGIVLLSEGPSSRVKKMLKDEEHRVGRV
                     VPNVPVHLIEVGRDEGQVSLTKLQTAVRKLPKKLTGEEVASVSHRLRSLGGTRPPIPK
                     GIDPMRARPNRKASRGR"
     gene            1184..>2211
                     /locus_tag="VFC06_01480"
     CDS             1184..>2211
                     /locus_tag="VFC06_01480"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZL80605.1"
                     /translation="MAAASVGDVGGEPVIRGYEVSGPVGFGASGTTLGVRGQDGRPWV
                     ASIVRRDARLGRLRLLRDLHHPHLPAIHDMVEVGDGEAAVVMDLVVGPSLATLVNARS
                     WLRGPEVVTVWRAIADALAAMHQRGLVHGDVSPANIIIGPDGRPVLIDVVGHGGAERG
                     HTGYVPPELDDGDATTASDVWSLARTLSWASGEDRGVLAALGDALADAPARRPSARDF
                     ATWAFVLGTPGNIDVPGAASLAGAQLRAGARQTVLTPARPRPRLRGRWAWTLVTALLA
                     VLGVIGGFVLLSATVAVDTRPAATPRAIRAFPALPAESAEAVTRELLDLHDRVLVERD
                     ATALASVYT"
BASE COUNT          308 a          759 c          787 g          357 t
ORIGIN      
        1 catcggtgta cgggtcgacg atcgacatgt tgacgttcag cgtcttctcg gcccggaacg
       61 gatcgaggta ggccgtcgag atgtccgcca ggagcttcat gtcggactcg tggatcgcct
      121 ggaacccacg gatcgacgag ccatcgaaca tcagcccttc ggtgaacacg tcgtcggtga
      181 acgcttgaac gggaatgttg aagtgctgca tcactccggg caggtcacag aagcgcacgt
      241 cgatgaattc gacggcctcc tcgcgtgtgt aggcgatggc ctcttcggcg tttgaaaaca
      301 tccactgctc ctcagtcatt tctaggcggg gacattccaa cggtagcgac cggcagttac
      361 cccggtgtca ccctgacatg acgggagtgt tacggggagg gggtggacgg tgacgtccgc
      421 cacaccggtc aacgtcccct cgacgccttg cggttgggcc gcgcgcgcat cgggtcgatg
      481 cccttgggga tcggggggcg ggtcccgccg agcgagcgca gccggtgcga gacggaggcc
      541 acctcttccc cagtgagttt cttgggcagc ttgcgcaccg ccgtctgcag cttggtgagg
      601 gacacctgcc cctcatcgcg ccccacctcg atcaggtgga ccggcacgtt cgggaccacg
      661 cgccccaccc tgtgctcctc gtccttgagc atcttcttta cgcgggagga ggggccctcc
      721 gagaggagca cgatgcccgg ccgccccacc atccggaaca cgaggtcctg ggtgcgcggg
      781 ttgaccgcta cgggctcctg ctcgatgttc cacccgcgtt tgatctggtc gagcaccgcg
      841 atcgaggcgc caggctggcc ctcgatctgg gcgtaggaga ccttcttgac gcgccacgag
      901 aggatgaaca tcgccaccgt gaggccggcc atcgccccga ggaacccgac gtagatgggg
      961 tgaccgacgg cgtacccgat ccccacaccg atcccggctg acgcgaggag cccccccgcc
     1021 atcgcccagc cgacccacgg ctctgcgccc gcggtgagcc ggtaggcgtc gatgaagttg
     1081 ttgtaccaac gtcgcttctt gggcttgggc gtgggtgcgg gcgcggagtt cgacatgacg
     1141 tcagattcta tccggtcctg tggatggaag gagcgcgctg tgggtggctg ccgctagcgt
     1201 cggtgacgtg gggggagaac cggtcattcg cgggtacgag gtcagcgggc ccgtgggctt
     1261 cggggcgtct ggcaccacgc tcggtgtccg cggccaggac ggtcggccgt gggtcgcgag
     1321 catcgtgcgc cgggacgctc ggctcggccg cctccgcctg ctgcgggacc tgcaccaccc
     1381 ccacctgccc gccatccacg acatggtgga ggtgggcgac ggcgaggccg ctgtcgtcat
     1441 ggacctggtg gtggggccct cactggccac gctcgtcaac gcgcggtcat ggctgcgggg
     1501 acccgaggtg gtcaccgtgt ggcgcgccat cgccgacgcg ctggccgcaa tgcaccaacg
     1561 ggggctggtc cacggcgacg tctcgcccgc caacatcatc atcgggccgg acggcaggcc
     1621 ggtcctcatc gacgtggtgg gccacggtgg ggccgagcgc ggccacaccg gctatgtgcc
     1681 tcccgagttg gacgacggcg acgccacgac ggcgtcggac gtctggtcgc tcgcccgcac
     1741 gttgtcgtgg gcgagcggcg aggaccgcgg cgtgctcgcc gccctggggg acgccctcgc
     1801 ggacgccccg gcgcggcggc ccagcgcccg ggacttcgcc acctgggcat tcgtgctggg
     1861 gacgcccgga aacatcgacg tcccgggcgc ggccagcctc gcgggcgccc agctgcgcgc
     1921 cggtgcccgg cagacggtgc tgacgccggc ccgtccccgc ccgcgcctcc gcggtcggtg
     1981 ggcgtggacg ctcgtgacgg ccctgctcgc ggtgctgggc gtgatcggcg ggttcgtcct
     2041 cctctcggca accgtggcgg tggacacccg ccccgcagcg acgccccgcg ccatccgcgc
     2101 attcccggcg ttgccggcgg aaagcgccga ggcggtgacg cgcgagctgc tcgaccttca
     2161 cgaccgggtg ctcgtggaac gggatgccac ggcccttgcg tcggtgtaca c
//
LOCUS       DASRKH010000089         3942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_24163, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000089 DASRKH010000000
VERSION     DASRKH010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3942
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24163"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..654)
                     /locus_tag="VFC06_01485"
     CDS             complement(46..654)
                     /locus_tag="VFC06_01485"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HZL80606.1"
                     /translation="MTPALTLERFETPLGDLSVFASEDGVVRAAGFGPGAQVAAQLPL
                     DLLADGWSPGTLPHVREAVEAWLDGDGAAIATVPVSQEGGPFFQDVWEATRGIQPGQP
                     QSYHEVAAAAGRPRAMRAAGTACGRNSIALFVPCHRVIASGGRLGQYGPGGVGIKAAL
                     LAFEAGWSDAAVLRAGREAHPDDAAPAVGVERLGAGDAGGGR"
     gene            complement(805..1011)
                     /locus_tag="VFC06_01490"
     CDS             complement(805..1011)
                     /locus_tag="VFC06_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BldC family transcriptional regulator"
                     /protein_id="HZL80607.1"
                     /translation="MADTLHDPEKLLTPSEVAAIFRVDPKTVTRWAKVGKLSSIRTLG
                     GHRRFRELEVRTLLAEAQDQAHSA"
     gene            1232..1405
                     /locus_tag="VFC06_01495"
     CDS             1232..1405
                     /locus_tag="VFC06_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3073 family protein"
                     /protein_id="HZL80608.1"
                     /translation="MGRGRQKAKDAKIARQLKYTDHSSDLRELERELIGHSSVPAQQS
                     EDDGHDRWSDDDD"
     gene            complement(1454..2560)
                     /gene="purM"
                     /locus_tag="VFC06_01500"
     CDS             complement(1454..2560)
                     /gene="purM"
                     /locus_tag="VFC06_01500"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HZL80609.1"
                     /translation="MSDDGITYASAGVDTHAGDRAVELMKEAVGKTHGPEVMGGVGAF
                     AGLFDASALKNYRRPLLASSTDGVGTKIVIARAMDIHDTIGQDLVAMVVDDIVVCGAK
                     PLVMTDYIATGRVIPERIADIVRGIAEGCLMADTALVGGETAEHPGVMEPDDYDVAGA
                     AIGVVEADALLGPEKVAAGDVVVAMASSGLHSNGYSLVRSVMAHAAWTLDLHLSDLGR
                     TVGEELLEPTRIYAKLCVDLAATVPGLHAFSHVTGGGLAANLARVIPAGLSATVARDA
                     WQLPAIFNTIAGAGKVPMHDLESTLNMGVGMVSVVSASDADAVMAASAAAGVPAWELG
                     RIAADDGRAVSADVVRGAKGVQGGAVYLSGAYRA"
     gene            complement(2557..>3942)
                     /gene="purF"
                     /locus_tag="VFC06_01505"
     CDS             complement(2557..>3942)
                     /gene="purF"
                     /locus_tag="VFC06_01505"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HZL80610.1"
                     /translation="PGEEVAKLTYFGLYALQHRGQESAGIAASDGHKILVFKDMGLVS
                     QVFDEGALEALQGHIAVGHARYSTTGASTWANAQPTLGPTAHGTVALGHNGNLTNIQE
                     LIDLLVSREGMAKRLDLKGGKCTDTAIVTALLGSDQQDTLAETALDLLPHVRGAFSFV
                     FMDEHALYAARDPQGVRPLVLGELPSGGWVVASETAALDIVGARQVREVEPGELLIID
                     DEGVHSERFADSDPKGCIFEYVYLARPDTTIAGRSVHAARLEMGRQLAREFPVDADLV
                     IPTPESGTPAAVGYAQASGIPFGQGLVKNTYVGRTFIQPSQTLRQLGIRLKLNPLREV
                     IEGKRLIVVDDSIVRGNTQRAIVKMLRDAGAAEVHVRISSPPIQWPCFYGIDFPTREE
                     LVAVGHTMEDVCAQLGADSLGHISLEGMLAATKISESRLCTACFTGKYPIGPPADERH
                     QLVAQGAELVG"
BASE COUNT          649 a         1361 c         1307 g          625 t
ORIGIN      
        1 gcagccacat ccgcatcggc gcgtgctgcg gggccgtagc cccgctcatc ggccgcctcc
       61 cgcgtcgccg gcgcccagcc gctccacgcc gaccgccgga gcggcatcgt ccgggtgggc
      121 ctcccgcccc gcacgcagca ccgccgcgtc actccatccg gcctcgaatg cgaggagtgc
      181 ggccttgatt ccgacgccac ctggcccgta ctgccccagc cgtccgcccg acgcgatcac
      241 gcgatggcac ggaacaaaca gcgcgatcga gttgcgtccg caggcggttc ctgccgcccg
      301 catcgcccgc ggcctgcccg ccgctgccgc cacctcgtgg tacgactgcg gctgcccggg
      361 ctggatgccc ctcgtggcct cccacacgtc ctggaagaac ggcccgccct cttggctcac
      421 cggaaccgtg gcgatcgcgg cgccgtcgcc gtccaaccac gcctcgacag cctcccgcac
      481 gtgcggcaac gtgcctggag accacccatc agccagaagg tcgaggggca actgcgccgc
      541 aacctgcgcc ccgggaccga aaccggcggc gcgcactacg ccgtcctcgc tggcaaacac
      601 gctgagatca cccaagggtg tctcgaagcg ctcaagcgtc agggctgggg tcatgggcca
      661 aggttacggg tcagctgaga ccacgagtcg gagccacctg cgctccaggt ggcaccactc
      721 ggcggcgacg cgtacgcggg aggcccgcgg gcatgggaac ggcccccgtc cttgtggggc
      781 gggggccgtg ctcgttttca gaggttatgc ggagtgggct tgatcctgcg cctcggcgag
      841 cagagtacgt acctctagct cacggaagcg cctgtgacca cccagcgtgc gaatcgaaga
      901 gagcttgccg accttggccc aacgggtcac ggtcttgggg tcaacgcgga aaatcgcggc
      961 aacctcactt ggcgtgagca gcttctccgg gtcatggaga gtatcagcca tcattttctt
     1021 tccctcagtt atgccggtag cccagcgggt atcttcacaa catgagactt gcgacataga
     1081 tgattatgac gcggttccgg aaagatttcc cattttgttg gtatgtccga agattacgag
     1141 tggtccaacg aaggttgtag agtatcggca ggacttgaag cggaggaccg cacttttgcg
     1201 cgtcctcagt agtcagaagg gacgggcgat catggggcgc ggccgtcaga aggcaaaaga
     1261 cgcgaagatt gcgcgtcagc tcaagtacac cgaccacagc agcgatctgc gcgagttgga
     1321 acgcgagctc atcggtcact ccagcgtgcc tgcgcaacag tcggaggacg acggtcacga
     1381 ccgttggtcc gacgacgacg actgaacctg agcctgtagc gcctttcgtc ggcccaggaa
     1441 gcccgcgcgc gcactaggcg cggtaggccc cactcaagta cacggcgccg ccctggacgc
     1501 cctttgctcc ccggaccacg tctgcggata ccgcgcgccc gtcgtcggcc gcgattctcc
     1561 ccagctccca cgccgggacg cccgcagcgg ccgaggccgc catcaccgcg tcggcgtcgg
     1621 atgcggagac aacggagacc atcccgacgc ccatgttgag ggtcgattcg agatcgtgca
     1681 tggggacctt gccggcgccc gcgatcgtgt tgaagatcgc tggcagctgc cacgcgtctc
     1741 gagccacggt cgcggagagc cccgcaggga tgacgcgcgc gaggttcgcc gcgagtcccc
     1801 cgccggtcac gtgcgagaac gcgtggaggc cgggcacagt ggccgcgaga tccacacaca
     1861 gcttcgcgta gatgcgggtg ggctccagca gctcctcgcc gacggtccgc cccaggtcgc
     1921 tgaggtggag gtcgagcgtc cacgccgcat gcgccatgac ggaacgcacc agcgaatagc
     1981 cgttcgagtg cagaccggac gatgccatgg cgaccaccac gtcccccgcg gccaccttct
     2041 ccgggccgag cagggcatcg gcctccacta ctccgatggc ggcccctgcg acgtcgtagt
     2101 cgtcgggctc catcacgccc gggtgttcgg cggtctcgcc ccccacgagc gcggtgtccg
     2161 ccatcaggca accctcggcg atgccacgca cgatgtcggc gatgcgctcc gggatcaccc
     2221 tgccggtcgc gatgtagtcg gtcatgacga gcggcttggc gccgcacacc acgatgtcgt
     2281 ccacgaccat cgcgaccagg tcctggccga tcgtgtcgtg gatgtccatc gcgcgggcga
     2341 tgacgatctt ggtgcccaca ccatccgtcg acgaggccag cagcgggcgg cggtagttct
     2401 tgagagcaga cgcgtcgaag agtcccgcaa acgcgccgac tcctcccatc acctcggggc
     2461 catgggtctt gcccaccgcc tccttcatca gttccaccgc gcggtctcct gcgtgggtgt
     2521 cgacccctgc agaggcgtag gtgatgccgt cgtcgctcat cccacgagct ccgcgccctg
     2581 ggccaccaac tggtgacgct cgtccgcggg cggcccgatg gggtacttgc cggtgaagca
     2641 cgcggtgcac agccgggatt ccgagatctt ggtggccgcg agcatgccct cgagcgagat
     2701 gtggcccagg gagtccgcgc cgagctgggc gcacacgtcc tccatggtgt ggcccacggc
     2761 gaccagttcc tcacgcgtgg ggaagtcgat gccgtagaag cagggccact ggatgggcgg
     2821 cgaggagatc cgcacgtgca cctcggccgc gcccgcgtcg cgaagcatct tcacgatcgc
     2881 tcgctgagtg ttgccgcgca cgatcgaatc gtccaccacg atcagccgct tgccctcaat
     2941 gacctcgcga agtgggttga gcttgaggcg gatgcccaat tgacggagcg tctgcgacgg
     3001 ctggatgaag gtgcgtccga cgtaggtgtt cttcaccagc ccctggccaa acgggattcc
     3061 cgatgcctgg gcgtagccga cggcggcggg ggttcccgat tcgggcgtgg ggatcacgag
     3121 gtcggcgtcc acggggaact cgcgggccaa ctggcggccc atctcgaggc gcgcggcgtg
     3181 cacggagcgg ccggcgatcg tcgtgtcggg gcgggcgagg tacacgtatt cgaagatgca
     3241 gcccttgggg tccgagtccg cgaagcgctc ggagtggact ccctcgtcgt cgatgatgag
     3301 cagctcgccc ggctcgacct ctcgcacctg acgcgcgccc acgatgtcga gcgcggccgt
     3361 ctcggacgcc acgacccagc cgccggacgg cagctcgccc agcacgagcg ggcgaacgcc
     3421 ctgcgggtcg cgtgccgcgt acagcgcgtg ctcatccatg aacacaaagg agaaggcgcc
     3481 gcgcacgtgc ggcagcaagt caagtgccgt ctcggcgaga gtgtcctgtt ggtccgaccc
     3541 caacagcgcg gtcacgatgg cggtgtcggt gcacttgccg cccttgaggt cgagccgctt
     3601 ggccatgccc tcgcggctga ccagcaggtc gatcagctct tgaatgttgg tgagattgcc
     3661 gttgtgaccc aacgcgaccg tgccatgtgc cgtgggaccc agcgtcggct gggcgtttgc
     3721 ccaggtcgat gcgcccgtgg tcgagtagcg cgcgtggccc accgcgatat ggccctggag
     3781 tgcctcgagc gcgccctcgt cgaacacctg cgacaccagg cccatatcct tgaagacgag
     3841 gatcttgtgg ccatccgagg cggcaatgcc cgcggactcc tgaccacggt gctgcaacgc
     3901 gtagagacca aagtaggtga gcttggcgac ctcttcgccc gg
//
LOCUS       DASRKH010000090         2165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_36605, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000090 DASRKH010000000
VERSION     DASRKH010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36605"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="VFC06_01510"
     CDS             <1..780
                     /locus_tag="VFC06_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015771428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZL80611.1"
                     /translation="SIASVIDETTKVVAFTAASNVTGIITDVAPIVAKAREVGALTFL
                     DACQAVPHLPVDVQALGVDFVAFSGHKMLGPTGIGALWGRKDLLDVLPAVVFGGGSIK
                     TVTMEATTWLDAPHRFESGTQPVAQAVGLGAAAEYLMAFGMDNVARHEARLAQIMREG
                     VSELPGIRLLGPVGDVTQTDVLGIASVVVDGVHAHDVGQILDDAGIAVRVGHHCNQPL
                     HRRFGVASSTRASAHIYTTEDEARAFVDVLATVRTFFGVRS"
     gene            777..1241
                     /locus_tag="VFC06_01515"
     CDS             777..1241
                     /locus_tag="VFC06_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003071387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HZL80612.1"
                     /translation="MSDLEQMYQQVILDYAKTRRGNGLVTPDAPAVGESHQVNTTCGD
                     DITLRVGLDGDRITSLSWEGQGCSISQASASMLSELMEGADLRTADETIEAFKALMHS
                     KGVPLGDDREDLLEDATAFVGVGKYPARIKCALISWMALRDAIARATVGTEG"
     gene            1247..1573
                     /locus_tag="VFC06_01520"
     CDS             1247..1573
                     /locus_tag="VFC06_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HZL80613.1"
                     /translation="MTDTAHPANVADVEEALRDVIDPELGINVVDLGLIYGVQLDENQ
                     HAIIDMTLTSAACPLTDVLEDQTRECLEGLVDGFRINWVWMPPWGPDKITDDGREQLR
                     ALGFNV"
     gene            complement(1604..>2165)
                     /locus_tag="VFC06_01525"
     CDS             complement(1604..>2165)
                     /locus_tag="VFC06_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HZL80614.1"
                     /translation="PGVVVFDQATSTACGTASSATGPFYCPPDQTLYADTTFFSSLTQ
                     FGFEGGNLAEAYVIAHEYGHHVENELGIFDIADRSGTGTNSDSVKIELMADCLAGVWV
                     GHAATTPDPDTGIPFLEPITQAQLDNALAAAASVGDDRIQETIQGEATPHTFTHGTSE
                     QRTQAFTTGYTNGTVASCDAFGVLNR"
BASE COUNT          361 a          695 c          733 g          376 t
ORIGIN      
        1 tcgatcgcct cggtgatcga tgagaccacg aaggtggtcg cgttcacggc agccagcaac
       61 gtcaccggga tcatcacgga cgtggcccca atcgtggcca aggcccgcga ggtgggcgcg
      121 ctgaccttcc tcgacgcgtg ccaggccgtt ccccatcttc ccgtcgacgt gcaagcgctc
      181 ggcgtggact ttgtggcctt ctcgggccac aagatgctcg gccccaccgg catcggagca
      241 ttgtggggcc gcaaggatct gctggacgtg cttcctgccg tcgtctttgg cggcggatcc
      301 atcaagacgg tgacgatgga agccaccacc tggctcgacg ccccgcatcg cttcgaatcc
      361 ggcacgcaac ccgtggcgca ggccgtgggc ttgggagctg ccgccgagta cctgatggcc
      421 ttcggcatgg acaacgtcgc gcggcacgag gcgcggctgg ctcagatcat gcgtgagggc
      481 gtgtccgaac tgcctggtat tcggctactc ggaccggtgg gcgacgtcac tcagacggac
      541 gtgctgggca tcgcgtccgt ggtggttgac ggcgtgcatg cccacgacgt gggacagatt
      601 ctcgacgacg ccggcatcgc cgtgcgcgtc ggccaccact gcaaccagcc tctgcatcgg
      661 cgcttcggcg tcgcgagctc gacccgcgcg agcgcgcaca tctacacgac cgaggacgag
      721 gcgagggcgt ttgtcgacgt gctcgccacc gtccggacat tctttggggt ccgctcatga
      781 gtgacctcga acagatgtat cagcaggtca tcctcgacta tgcgaagact cgtcgcggca
      841 acgggctggt cacgcccgat gccccggcgg tgggggagtc gcatcaggtc aacacgacat
      901 gtggcgacga catcacgctg cgcgtgggac tcgatggcga tcgcatcacg tcgttgtcgt
      961 gggagggcca ggggtgctcc atctcccagg cctccgcatc gatgctgtcc gagctcatgg
     1021 agggcgccga ccttcgcacc gcggacgaga cgatcgaggc cttcaaggcc ctcatgcaca
     1081 gcaagggcgt tccccttgga gacgatcgcg aagacctctt ggaagacgcc accgcctttg
     1141 tgggcgtagg aaaatacccg gcgcgcatca agtgcgccct catcagctgg atggctttgc
     1201 gcgacgcgat tgcgcgcgcc accgtcggca cggaaggata ggcaccatga ccgacactgc
     1261 acaccccgcc aacgtggccg acgtcgaaga ggcgctccgc gatgtgatcg acccggaact
     1321 cgggatcaac gtcgtcgact tgggcctcat ctacggcgtt caactcgatg agaaccagca
     1381 cgccatcatc gacatgaccc tgacgtcggc ggcgtgcccg ctgaccgacg tgctcgagga
     1441 ccagacgcgc gagtgccttg aggggctcgt cgacggcttc cggatcaact gggtgtggat
     1501 gccgccgtgg ggcccggaca agatcaccga cgacggccgc gagcagttgc gggcgctggg
     1561 cttcaacgta tagcgcgctc taccgtcgag cgggcttgac cgtctagcgg ttcagcactc
     1621 cgaacgcgtc gcatgacgcg acggtgccgt tcgtgtaccc ggtggtgaac gcctgcgtgc
     1681 gctgctcgga cgtgccgtgc gtgaaggtgt ggggggtggc ctctccctgg atcgtctcct
     1741 ggatcctgtc gtcacccact gatgccgccg ccgcgagcgc attgtcgagc tgagcctgag
     1801 tgatcggctc caggaacgga atgccggtgt cggggtccgg ggtggtggcc gcgtggccca
     1861 cccacacgcc agcgaggcag tcggccatca gctcgatctt gacggagtcc gagttggttc
     1921 cagttccaga gcggtccgcg atgtcgaaga tgcccaactc gttctccacg tggtggccgt
     1981 actcgtgggc gatgacgtag gcctcggcca agttgccgcc ttcgaacccg aactgggtga
     2041 gagacgagaa gaacgtcgtg tccgcgtaca gcgtctggtc gggcgggcag tagaacgggc
     2101 cggtcgccga tgatgcggtc ccgcatgcgg tcgatgtggc ctggtcgaag acgaccacgc
     2161 cgggc
//
LOCUS       DASRKH010000091         2183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_45691, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000091 DASRKH010000000
VERSION     DASRKH010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45691"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VFC06_01530"
     CDS             <1..432
                     /locus_tag="VFC06_01530"
                     /inference="COORDINATES: protein motif:HMM:NF014669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015225 - biotin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015878 - biotin transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HZL80615.1"
                     /translation="APIFAGRSAGPAVWTGPTAGFLAGFVAAAFVAGWLVRRMRRRGQ
                     LTVSGVIGACAVASLLVLNLIGWSFFMLRLGTTAADTVAFATPFLVGDIIKVFVAGVT
                     AAAVHRAYPGLLGVPVSPTNVEARETPATPPAVSLGAQSAS"
     gene            449..1153
                     /locus_tag="VFC06_01535"
     CDS             449..1153
                     /locus_tag="VFC06_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZL80616.1"
                     /translation="MIEFKDATVVAPDSGITILKPTTLSLSEKRITIIGANGSGKSTL
                     ARLINGLAIPVSGTVTVDGIDTAKHGARVRRKVGFLFTDPTSQLIMPTAVEDVMLSLR
                     RVIRSKNERVKAALAALEDIGLADKGDVSVNALSGGQRQLLALAGVLTATPQVVVADE
                     PTTLLDLRWKAHVGALLHSLPIQLIEVTHDLEAATRAQRTLVVDDGGVVFDGNPVDAV
                     AHYRDLMYRKSVGKST"
     gene            1150..1815
                     /locus_tag="VFC06_01540"
     CDS             1150..1815
                     /locus_tag="VFC06_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HZL80617.1"
                     /translation="MTHVLGMYRHGNSPLHRLPAWSKVATLIVWSAFTLIIRDPITSG
                     GLAAAALLLLVSVLPAPGPTLRGLGMIVIVAATASAYQVWKGEYVAAIDLGVDLIGLF
                     ALSLAITGSTPMSDMLDLATTAARPLRRILPPAVTGLMFAIMLRAIPEIAGIMRQSRD
                     AARARGIRRGPTAFMIPTATRTVGFALDLGAALHARGIGDDATSDAPRARPMRIATPD
                     PNG"
     gene            1879..>2183
                     /locus_tag="VFC06_01545"
     CDS             1879..>2183
                     /locus_tag="VFC06_01545"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HZL80618.1"
                     /translation="MTFSEGTRLDPNRVQRRGAGGSRGGVAIGGGIGGGIGGIIIVIL
                     ALIFGIDPSALNLDPGTQGLGGAAGAQDDTAQQDFAASCTSGADANADADCRVLAVI"
BASE COUNT          316 a          786 c          719 g          362 t
ORIGIN      
        1 gccccgatct ttgccggccg atccgccggg cccgccgtgt ggaccggccc cacggccgga
       61 ttcctcgcag gcttcgtggc cgcagccttc gtcgcgggct ggctggtgcg caggatgcgc
      121 cgacgcggcc aactcacggt gtctggcgtg atcggcgcgt gcgccgtcgc ctcgctgctg
      181 gtcctcaacc tcatcggctg gagcttcttc atgctgcggc tgggaaccac ggcggccgac
      241 actgtcgcgt tcgccacgcc gttcctggtc ggcgacatca tcaaggtgtt cgtcgccggg
      301 gtgacggccg ctgcggtgca ccgcgcgtac ccggggctgc tcggcgtacc ggtttccccc
      361 accaacgtcg aggcacgaga gacgcccgcc acacccccag cggtctccct cggcgcccag
      421 agcgcctcgt agcgcttacc ctttcctcat gatcgaattc aaggacgcga ccgttgtcgc
      481 gcccgattcc gggattacca tcctgaagcc caccacgctg agcctgagcg agaagcgcat
      541 caccatcatc ggtgccaacg gctcgggcaa gtcgacgctc gcgaggctga tcaacggcct
      601 ggcaatcccc gtgtcgggga ccgtcacagt ggacggcatc gacacggcca agcatggagc
      661 tcgggtgcga cgcaaggtcg gcttcctgtt caccgacccg acgtcgcaac tcatcatgcc
      721 caccgccgtc gaggacgtca tgctgtcgct gcgccgcgtg attcgatcca agaacgagcg
      781 cgtgaaggct gcgttggcgg ccctcgagga catcggcctg gcagacaagg gcgacgtgtc
      841 ggtcaacgcg ctgtcgggcg gacagcgcca gttgctggcc cttgcgggcg tgctcacggc
      901 gacgccccag gtggtcgtgg ccgacgagcc cacgacgctc ctcgatctgc gctggaaggc
      961 gcacgtgggc gcgctgctgc actccttgcc gatccagctg atcgaggtga ctcacgacct
     1021 cgaggccgcg accagagcgc aacgcaccct ggtggtcgac gacggcggcg tggtattcga
     1081 cggcaacccg gtcgatgcgg tggcgcacta tcgggacctc atgtaccgca agtccgtcgg
     1141 gaagtcgacg tgacgcacgt cctgggcatg taccggcacg gcaactcgcc gctgcaccga
     1201 ctgcccgcgt ggtccaaggt cgccacgctg atcgtgtggt cggccttcac gctgatcatc
     1261 cgtgacccga tcacctccgg cggtctggcc gccgctgcgc tcctgctgct cgtgagcgtc
     1321 ttgcccgcgc caggacccac cctccgcggc cttgggatga tcgtcatcgt ggccgcgact
     1381 gcgagcgcct accaggtgtg gaagggcgag tacgtggccg cgatcgacct cggcgtcgac
     1441 ctcatcgggc tctttgctct gtccctggcc atcaccggat cgaccccgat gtccgacatg
     1501 ctcgatctcg ccacgaccgc cgcacgtccg ctgcggcgca tccttcctcc tgccgtcacg
     1561 ggactcatgt tcgcgatcat gttgcgcgca attccggaga tcgcgggcat tatgcgccag
     1621 tcgcgagacg ccgcgcgcgc tcgcggcata cggcgcggcc ctaccgcttt catgattccc
     1681 acggcgacgc gcacggtcgg cttcgcgctc gacctgggtg ccgcgcttca cgcccgcggc
     1741 atcggggacg acgccacgtc cgacgcaccg cgtgcgcggc cgatgcggat cgccacacca
     1801 gacccgaacg ggtagttttg gggcacggcg ttcctgtggg ggccgtcggc cacgtgcagg
     1861 gctcagccgg gagacaccat gacattcagt gagggcactc gcctcgaccc gaaccgcgtg
     1921 cagcgccgag gcgcgggcgg ctcacgcgga ggcgtcgcca tcggcggcgg catcggcggc
     1981 ggcatcggcg gcatcatcat cgtgattctc gcgctgatct tcggaatcga tccctctgcg
     2041 ctcaacctcg atccgggcac ccagggcctg ggcggcgcgg ccggggcgca ggatgacacc
     2101 gcgcagcagg acttcgcggc cagttgcacc tccggtgccg acgcgaacgc ggacgccgac
     2161 tgccgcgtgc ttgccgttat cga
//
LOCUS       DASRKH010000092         2073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_7129, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000092 DASRKH010000000
VERSION     DASRKH010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7129"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VFC06_01550"
     CDS             <1..367
                     /locus_tag="VFC06_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80619.1"
                     /translation="MMAELAESGTMPATAPLAAAAATVAVSAPAQAAAAVATATASSG
                     PRPVVSLAEADQTLCTDCKTCYQEMPEIFEPVRVIVDGEARTVARIIPGALEQLQVTP
                     DVEARIKKIVANCDSEIIR"
     gene            364..>2073
                     /locus_tag="VFC06_01555"
     CDS             364..>2073
                     /locus_tag="VFC06_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZL80620.1"
                     /translation="MSTVTGGDIRQYLSAREALESTRQQLEELEKSEAVGSFNSQLAV
                     LRQHLASNPRQFRDMFISSGMGAVAAEFQKDELGAEFTRTLWDMLVRDDDGSTVVMRF
                     LWDLPLRLKRKFIRALDAHMTERYPMFAGLSEAWPAGNNIRPYIREPEVRATDFELVN
                     QGYLGYRSIGYSQREVDLFVWLEALRDKQCADSPCELGVLLEGRKERKGGCPVQIHIP
                     SMLDLLGKGKFREALELLEGCNPLPNVTGRVCPQEVQCQGVCAHLKRPIEIGQLEWFL
                     PQREKAVEPDSIVARYGGRPDPWRKAAKPPIAIVGSGPSGLINAFLLAAEGFPVTVFE
                     AFHELGGVLRYGIPEFRLPNELIDDVVAKIRLLGGRFVTNFVVGKTASLQDLRDAGFW
                     RIFVGSGAGLPQFMNVPGEHLLNVMSANEFLTRVNLMQARREDYDTPLPEVRGKRIMV
                     VGGGNTAMDAARTAHRLGGRVTIAYRRTQSEMPARVEELHHALEEGIELAVLRAPSEF
                     VGDDRTHFVKAAVLDIMQLGEPDASGRRRPVATGEKQTVRTDLVIMALGNASNPIIKD
                     SQPD"
BASE COUNT          338 a          722 c          722 g          291 t
ORIGIN      
        1 gatgatggcg gagctggccg agtccggcac gatgcccgcc accgcaccgc tcgcggcggc
       61 cgcggccacc gtggccgtga gtgcgcccgc gcaggccgcc gccgcggtgg ccactgcgac
      121 ggcgtcgtcg ggaccgcggc cggtggtctc cctggcggag gccgaccaga ccctgtgcac
      181 cgactgcaag acgtgctacc aggagatgcc ggagatcttc gagcccgtgc gcgtgatcgt
      241 cgacggcgag gcgcggacgg tggcccggat catcccgggg gcgctcgagc agctgcaggt
      301 gaccccggac gtcgaggcgc gcatcaagaa gatcgtggcc aactgcgact ccgagatcat
      361 ccgatgagca ccgtgaccgg cggggacatc cgccagtacc tcagcgcgcg ggaggcgctc
      421 gagagcacga ggcaacagct cgaggagctg gagaagtccg aggccgtggg atcgttcaac
      481 tcccagctcg ccgtgctgcg ccagcacctc gcgagcaacc cgcgacagtt ccgcgacatg
      541 ttcatcagct ccgggatggg cgcggtggcc gcggagttcc agaaggacga actcggtgcc
      601 gagttcaccc gcaccctgtg ggacatgctg gtgcgggacg acgacggttc caccgtggtg
      661 atgaggttcc tctgggacct gccgctgcgc ctgaagcgca agttcatccg cgcgctcgac
      721 gcccacatga ccgagcggta cccgatgttc gcgggcctgt cggaggcctg gccggcgggg
      781 aacaacatcc ggccgtacat ccgcgaaccc gaggtgcgcg cgacggactt cgagctcgtc
      841 aaccagggct acctgggcta ccggagcatc ggctacagcc agcgggaggt ggacctgttc
      901 gtctggctcg aggcgttgcg cgacaagcag tgcgccgaca gcccgtgcga actcggtgtc
      961 cttctcgagg gccggaagga gcgcaagggc ggctgcccgg tgcagatcca catcccctcg
     1021 atgctcgacc tgctgggcaa gggcaagttc cgggaggccc tggaactcct cgagggctgc
     1081 aaccccctcc cgaacgtgac cgggcgggtc tgcccccagg aggtgcagtg ccagggcgtg
     1141 tgcgcccacc tcaagcggcc gatcgagatc ggccagctgg agtggttcct gccccagcgg
     1201 gagaaggccg tcgagcccga ctcgatcgtg gcccggtacg gcgggcgccc ggacccctgg
     1261 cgcaaggccg cgaagccccc gatcgccatt gtgggttccg gtccctcggg cctgatcaac
     1321 gcgttcctcc tcgcggcgga gggcttcccc gtcaccgtgt tcgaggcctt ccatgagctc
     1381 ggcggcgtcc tccggtacgg catccccgag ttccggctgc ccaacgagct catcgacgac
     1441 gtcgtcgcca agatcaggct gctcggcggc cggttcgtca cgaacttcgt ggtgggcaag
     1501 accgccagcc tccaggacct gcgcgatgcc ggcttctggc gcatcttcgt gggcagcggc
     1561 gcgggcctcc cccagttcat gaacgtgccc ggggaacacc tgctcaacgt catgtccgcg
     1621 aacgagttcc tcacccgcgt gaacctgatg caggcgcggc gggaggacta cgacacccca
     1681 ctgccggagg tgcgcggcaa gcgcatcatg gtcgtcggcg gcggcaacac cgccatggat
     1741 gcggcccgaa ccgcccaccg cctcgggggt cgcgtcacca tcgcgtaccg ccgcacgcag
     1801 agcgagatgc ctgcccgggt ggaggagctg caccacgcac tcgaggaggg catcgagctg
     1861 gcggtgctcc gcgcccccag cgagttcgtg ggcgacgaca gaacgcactt cgtcaaggcg
     1921 gccgtgctcg acatcatgca actcggggag ccggacgcgt ccggacgtcg ccgcccggtc
     1981 gcaaccgggg agaagcagac cgtgcgcacc gacctcgtga tcatggcgct cggcaacgcg
     2041 tcgaacccga tcatcaagga ctcgcagccg gac
//
LOCUS       DASRKH010000093         3074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_45664, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000093 DASRKH010000000
VERSION     DASRKH010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45664"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VFC06_01560"
     CDS             <1..316
                     /locus_tag="VFC06_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80621.1"
                     /translation="PRTRDEHVLAHKRKLQRRVHSVAERIEDRGQVEDGTLPLSAPRL
                     EREVSRLDRTGRRVVSALLFGALLIAGAIVRPEDAGLGTGLMVASSLPFIHALFGGRR
                     GR"
     gene            378..1172
                     /locus_tag="VFC06_01565"
     CDS             378..1172
                     /locus_tag="VFC06_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HZL80622.1"
                     /translation="MRRDVTAQLTVDVSRPSELAMSFAVADRSGGVEETLDVRLDGAP
                     LEASVIGDAHGTRLHVIRAEVGVLSIAYAATIEGREPPAPSAERDRLRYLRPSRYCES
                     DELAATANAEFGGLTGYALLEGVSSWVGTRLDYVPGSSLPTDGAVRTLLARQGVCRDY
                     AHLCVALLRASGVPARLVSVYAPGLDPMDFHAVCEAYVDGMWCAVDATALAPRSSLVR
                     IATGRDAADTAFMTVVSGHADLIDMVVGAVADVLPDDDMMAATHLG"
     gene            1567..2619
                     /locus_tag="VFC06_01570"
     CDS             1567..2619
                     /locus_tag="VFC06_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018779698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HZL80623.1"
                     /translation="MKALVYKGPGEKSWEDVPRPVIKDSTDVVVKMVATTICGTDLHI
                     LKGDVPEVEAGRILGHEGIGVITEVGPGVTQLAVGDRVILACVSSCGKCSNCRKSLFS
                     HCLDPEGVAGIGWIFGYMIDGTQAEYVRVPFAENSVYKVPEGMTDAEGILLSDILPTG
                     FEMGVQYGHVQPGDVVAVIGSGPVGLAAVMTARLYGPSKIIAVDLDAARLARAKDFGA
                     TDTVNSGDADWKEQVLALTDGAGVDVAIEAVGIPLTFTMATQVVRPGGNVANIGVHGE
                     AVDLPLNELWIRNIGISMGLVNTNTLGMLLKLVAEHKLPADKFVTHEFTFDQMIEAYD
                     VFGHAADHDALKVLIR"
     gene            2759..>3074
                     /locus_tag="VFC06_01575"
     CDS             2759..>3074
                     /locus_tag="VFC06_01575"
                     /inference="COORDINATES: protein motif:HMM:NF013883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_function: GO:0003997 - acyl-CoA oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZL80624.1"
                     /translation="MECTSANRLAGRFADTTHRMRVASKNRGDTLDAFNTLGGHVHFV
                     ACAHMERVILASFMAAIEECHNAEVRDVLNRACSLYVLQSIFEDRAWFMEYHRITAVR
                     SKA"
BASE COUNT          522 a          981 c         1031 g          540 t
ORIGIN      
        1 gccccgcacc cgtgatgagc acgtcctcgc ccacaagcgg aaacttcagc gccgtgtgca
       61 cagcgttgcc gagcggatcg aagatcgcgg ccaggtcgag gacggcaccc tgccgctgag
      121 cgcgccgcgg ttggagcgcg aggtttccag gttggaccgc acggggcggc gcgtcgtatc
      181 ggccctgctg ttcggcgccc tgctcattgc cggagccatc gtgcgaccgg aagacgctgg
      241 cctgggcacg gggctgatgg tcgcgtcgtc gcttccgttc attcacgcgc tgttcggcgg
      301 gcgtcgaggg cgctgacctc ccgtagggga gagcccctat cgccacgaac gagtgcaccg
      361 cgtgacacga tggcggcatg cgccgcgatg tcacagcaca gttgactgtc gatgtgtcgc
      421 gcccgagcga gttggcgatg tccttcgcgg tggccgaccg cagcggaggc gtcgaggaga
      481 cacttgatgt gcgactcgac ggtgcaccgc ttgaggcgag tgtgatcggc gatgcacatg
      541 ggacgcgact ccacgtgata cgcgccgagg tgggcgtcct gtccatcgcg tacgccgcaa
      601 ccatcgaggg ccgcgagccg cccgcgccat cggcggagcg agaccgcttg cgctaccttc
      661 gccccagccg gtactgcgag tccgatgagt tggcggcgac ggcgaacgcc gagtttggcg
      721 gcctgaccgg ctacgcgttg cttgaagggg tgagctcgtg ggtcggcacg cggttggact
      781 atgtgcccgg atcgagcctt cccaccgacg gtgccgtcag gacgctgcta gcccgtcaag
      841 gcgtgtgccg tgactacgcg cacctctgtg tggcgctcct cagagcgagc ggggttcccg
      901 cgcggctcgt ctccgtctat gcgcccggct tggatccgat ggactttcac gccgtgtgcg
      961 aggcgtacgt cgacggcatg tggtgtgcgg tcgacgccac ggcgctggcg ccgcgcagct
     1021 ccctggtgcg catcgcgacc ggcagggatg cggcagacac ggcgttcatg acggtggtct
     1081 cgggccacgc cgacctgatc gacatggtgg tgggtgcggt ggccgatgtg ttgccagacg
     1141 acgacatgat ggccgctact cacttgggtt gatgcggtag tcgtgcgagc ggctccgctg
     1201 agctccacaa gatgagcgca agcgagacgc cctgctcacg cacgtacaac ggcggcgggc
     1261 tgcgaccacg ctagggggcc ggctctcgcg cgcgggcgca atcttttgct agccgtcaca
     1321 tcaaccgatg ccaacctcat cacagtattc gacgggaacc gtactagaag ttgcatctgg
     1381 ccgatatcgg gcgcattctg aaacagtccg caggccctcg cggcccaaac ctcgcctcct
     1441 ggctccccca gcatcagact tggcttcttc gcgtgagccc cacacgccat tgggaagaag
     1501 accatgactg caccagtaga tgccacagac acccccagcg cgaccggctc cacgagcggg
     1561 cacacgatga aggcactcgt gtacaagggc cccggagaga agagctggga agacgttccc
     1621 cgccctgtga tcaaagactc cacggacgtc gttgtgaaga tggtcgccac gaccatttgt
     1681 ggaacggacc tgcacattct caagggggat gtgccggaag tcgaggcggg ccgcatcctc
     1741 ggtcacgagg gcattggcgt catcaccgag gtcggcccag gcgtcactca gctggcggtc
     1801 ggcgaccggg tcatcctggc gtgcgtgagc tcgtgtggca agtgttccaa ctgccgcaag
     1861 agcctgttca gccactgcct cgaccccgaa ggcgtcgctg gcatcggctg gatcttcgga
     1921 tacatgattg acggcacgca ggccgagtac gtgcgcgttc cgttcgccga gaactcggtc
     1981 tacaaggttc cggaagggat gacggacgcg gagggaatcc tcctgtctga catcctgccg
     2041 acgggattcg agatgggcgt ccagtacggg cacgttcagc cgggcgacgt cgtcgccgtg
     2101 atcggttccg gtcccgtagg cctcgccgcc gtgatgacgg cgcgactgta tggaccgtcg
     2161 aagatcatcg ccgtcgacct tgacgcggcg cgcctggccc gtgccaagga cttcggcgca
     2221 accgacaccg tgaactccgg tgacgccgac tggaaagagc aggtccttgc cctcacggac
     2281 ggcgcgggcg tcgacgtcgc aatcgaggcg gtagggattc cgttgacatt cacgatggca
     2341 acccaggtgg tgcgccccgg cggtaacgtc gccaacatcg gcgtccacgg tgaggctgtg
     2401 gatttgcccc tcaacgaact gtggatccgg aacatcggca tctccatggg gctcgtcaac
     2461 acgaacacgc ttggcatgct gctcaagctg gttgccgagc acaagttgcc cgcggataag
     2521 ttcgtcaccc acgagttcac cttcgaccag atgatcgagg cctacgacgt gtttgggcac
     2581 gccgctgacc acgacgcact caaggtgttg atccgctagc gccggggccc tcaacatttt
     2641 cgcagacgtg acgtgatgaa ggcgtgcgcc gcctggtgtg cgctgatctg atcgtcgagg
     2701 tgtcgactcc tgccggtgtg gtattcgcca tcgccgtacc gcgcaggagt gggcgctgat
     2761 ggagtgcacc tccgcgaacc gcctggccgg gcggttcgcc gacaccacac accgcatgcg
     2821 ggtggcaagc aagaaccgcg gggacacgtt ggacgccttc aacaccctcg gcggccacgt
     2881 gcatttcgtc gcctgcgccc acatggagcg cgtgatcctc gcgtccttca tggcggcgat
     2941 cgaggagtgc cacaacgccg aggtgcgcga cgtgctcaat cgcgcttgca gcctgtatgt
     3001 gttgcagagc atcttcgagg accgtgcgtg gttcatggaa taccaccgca tcactgcggt
     3061 gcggtccaag gccg
//
LOCUS       DASRKH010000094         2412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_213, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000094 DASRKH010000000
VERSION     DASRKH010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VFC06_01580"
     CDS             <1..429
                     /locus_tag="VFC06_01580"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZL80625.1"
                     /translation="PLMRAMGRRRREEVLARSSFGEVARTFEPGAEIPGLPDWECIPT
                     PGHTPGHVSYFRPRDRVLISGDALMTLKVNTLQGLLLQQSGLSGPPWYTTWSKHAARE
                     SIRTLARLEPTVLVGGHGAPLTDPGTAARLRAFAGLGATA"
     gene            complement(490..858)
                     /locus_tag="VFC06_01585"
     CDS             complement(490..858)
                     /locus_tag="VFC06_01585"
                     /inference="COORDINATES: protein motif:HMM:NF018574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HZL80626.1"
                     /translation="MDLESLVEQQRQKTAETWSVLLDHGLREGSPLDVETFFFADDEA
                     AAVSLAEALIAEGTTASTESFLARAGLFGKKTVWSVQADSALPAASLEALSDLTEAMV
                     RHAVRAGAVYDGWGAQIPES"
     gene            complement(885..1169)
                     /locus_tag="VFC06_01590"
     CDS             complement(885..1169)
                     /locus_tag="VFC06_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80627.1"
                     /translation="MDSTPEPSNGHAQAWRWWLAAFGLAVVASPVMVWGIFGTPATWA
                     AIAAAVALGLGMVCFVVATILKSRADGVHPLRVMGRALWAPIRFLIEFTF"
     gene            1454..1777
                     /locus_tag="VFC06_01595"
     CDS             1454..1777
                     /locus_tag="VFC06_01595"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZL80628.1"
                     /translation="MPVTLSCGYWGHAPGHVSYFRPRDRVLISGDALMTLRVNTVSGL
                     LLQQPGLSGPPWCTTWSQRAARESIRTLARLEPSVLAGGHGAPPTDPGPAARLRAFAG
                     LAGAV"
     gene            1794..>2412
                     /locus_tag="VFC06_01600"
     CDS             1794..>2412
                     /locus_tag="VFC06_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015884380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HZL80629.1"
                     /translation="MLTVPVPTSRPLPKVRASALTGRGWLNTGGRDVTLADLRGKVVV
                     LDFWTFCCINCLHVLDELRTLEEQYRDVLVIVGVHSPKFLHEADPVALAAAVDRYEVR
                     HPVLDDPDLTTWSAYTARAWPTLVVIDPEGYVVAHMAGEGHLHNLEVLVAELVRTHEA
                     TGTLHRGDGPYVPPEPTSGTLRFPAKAIALPGGNLLVADAGHHSLA"
BASE COUNT          365 a          911 c          791 g          345 t
ORIGIN      
        1 ccgctcatgc gggcgatggg ccggcggcgt cgcgaggaag tgctcgcccg gtcgagcttc
       61 ggcgaggtcg cccggacgtt cgaacccggc gccgagatcc ccggtctgcc ggactgggag
      121 tgcatcccga cgcccggcca caccccgggg cacgtctcct atttccgacc acgtgaccgc
      181 gtcctgatca gcggcgacgc cctcatgacc ctcaaggtca acaccttgca ggggctgttg
      241 ctgcagcagt cgggcctctc gggaccgccc tggtacacga cgtggagcaa gcacgccgca
      301 cgggagtcga tccgcacgct cgcgcgtctc gagccgaccg tgctggtcgg cggccacggc
      361 gcacccctga ccgatcccgg cacggcggct cgtctgcggg cgttcgcggg gctgggcgcg
      421 acggcctgag ggaaccgcgc agtccaggct tgggtggagg catcagcccc ggacggaggg
      481 tcgctcgccc taggactcag ggatctgggc gccccagccg tcgtagacgg caccggcgcg
      541 gacggcatgc cgcaccatgg cctccgtcag atcggacagc gcctccagcg aggcggcagg
      601 cagggccgag tcggcctgca cggaccacac cgtcttcttc ccgaagagcc cggccctggc
      661 cagaaacgac tccgtcgagg cggtcgtccc ctcggcgatg agagcctcgg cgagcgagac
      721 ggcggcggct tcgtcgtcgg cgaagaagaa ggtctcgacg tcgagcggcg aaccctctcg
      781 gagcccgtgg tcgagcagca cagaccacgt ctcggccgtc ttctgcctct gctgctctac
      841 cagcgactcc aggtccacgg gcctcaatct agggtgctcg gccgctagaa cgtgaactcg
      901 atcaggaagc ggatcggcgc ccacagagcc cggcccatga cacgcaacgg gtggacgccg
      961 tcggctctgg acttcaggat cgttgcgacg acgaagcaga ccattcctag cccgagagcc
     1021 acggctgcgg cgatggccgc ccaggtcgcc ggggtgccga agatgcccca caccatgacc
     1081 ggactggcga caacagcgag tccgaaggcc gccagccacc atcgccaggc ttgagcgtgg
     1141 ccgttcgatg gttcgggcgt gctgtccatg gactccatcc tcctcaccgc ttgacgcgca
     1201 agtggccgtt tcggcagtcg cgcgcaatcg aagcccggcc tcgaacagcg ccaacgccag
     1261 cacgtagtac acgaccaccc acgccggcag catccgccga cgctgctcac gagcgcgcgc
     1321 cgcctcgatc accgactcga ccaactcccg cgggaacgcc ttcgccagca cccccacact
     1381 gatccgatcc gccaacccac cgtcgtcgtc ctgcaccatc tgctgcccag ccctagccat
     1441 ccgcccaccc tacatgcccg tcacactaag ttgcgggtat tggggccacg ccccgggtca
     1501 cgtctcctac ttccgacctc gtgaccgcgt cctgatcagc ggtgacgccc tgatgaccct
     1561 cagggtcaac accgtgtcgg ggctcctgct ccagcagccg ggcctctccg ggccgccgtg
     1621 gtgcacgacg tggagccagc gcgcggccag ggagtcgatc cgcactctcg cgcgtctcga
     1681 acccagcgtg ctggccggcg gccacggcgc acccccgact gatcccgggc cggcggctcg
     1741 gctgcgggcc ttcgctgggc tcgccggagc ggtgtgacct cgctgcacgc ctggtgttga
     1801 ctgtcccggt gcccacgtcc cgaccgctgc ccaaggtccg agcgagcgcg ctgacgggac
     1861 gcggctggct caacaccggt ggccgcgacg tcacgctggc tgatctgcgc ggcaaggtcg
     1921 tcgtgctcga cttctggacc ttctgctgca tcaactgcct gcacgtcctc gacgagctgc
     1981 gcaccctcga ggagcagtac cgcgacgtgc tcgtcatcgt cggggtgcac tccccgaagt
     2041 tcctgcacga ggcggacccg gtcgcgctcg cggccgcagt cgaccgctac gaggtgcgtc
     2101 acccggtcct cgacgaccct gacctgacga cgtggtcggc gtacacggcg cgggcctggc
     2161 ccacgctcgt cgtgatcgac cccgaggggt acgtcgtcgc gcacatggcg ggcgaggggc
     2221 acctgcacaa cctcgaggtc ctggtcgcgg agctggtgcg cacgcacgag gccaccggga
     2281 cgctgcaccg cggcgacggc ccgtacgtcc cgcccgagcc cacgtcgggc acgctgcgct
     2341 tccccgcgaa ggcgatcgcg ctcccgggcg gcaacctcct cgtggccgac gccgggcacc
     2401 acagccttgc cg
//
LOCUS       DASRKH010000095         2489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_12271, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000095 DASRKH010000000
VERSION     DASRKH010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12271"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..2088)
                     /locus_tag="VFC06_01605"
     CDS             complement(70..2088)
                     /locus_tag="VFC06_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HZL80630.1"
                     /translation="MFRRLFWINLVLAIPVVLFSAMVQDWLGLSLSFPGSSWIPPVVG
                     TVIFIWGGRPFFTMAVPEIRNRQPGMMLLIALAITVAFLASLASTFGVGSLDFWWELA
                     TLIVVMLLGHWQEMKAVGQASGALAALAELLPDEAERVVGDDVETVRIADLVTGDVVV
                     VRPGARVPADGTIAQGSASMDESTITGESAPVRRAEGGRVVAGTVATDGSLRVEVTAV
                     GDDTALAGIQRMVTQAQESRSGARKLADRAAQALFYIALGSALVTFTVYAVQGDFSTG
                     IVRAVTVLVIACPHALGLAIPLVIAISTAKSAQAGVLVKDVLALETMRSISSVLFDKT
                     GTLTEGSHTVAEVEPLDDWDEERVLAVAAAVERDSEHPIGRAIVAAAAERDITVPDAS
                     DVDTLPGKGVVGTVDGDRVGVGGRSMLAELDIESPAAAVETRERWDGEGSPTLYVTVG
                     GTVVGLFTTHDPVRAASRATVDALHRQGVSVALITGDSQAVADRVARELGIDEVFAEV
                     LPQDKDSAVEKLQAGGGKVAMVGDGVNDAPALARANVGIAIGAGTDVAMEAAGVVLAS
                     SDPRGVLAIVALSRASHRKMIQNLVWATAYNALAIPVAAGVFSGIGFTLNPAAAAIAM
                     SLSTIVVALNAQLLRRVDLSYEALAPAGAAGAPHSEHQDHPEGAASSV"
BASE COUNT          396 a          846 c          863 g          384 t
ORIGIN      
        1 gcgtggaggc gtgcgatgcg gcgggtgata gagcggggtg cgatagtgat ggcgatgagt
       61 ccgccctcgt cacacggacg atgccgcccc ttcagggtga tcttggtgct cggaatgggg
      121 cgccccggcg gcgcctgcgg gcgcgagagc ctcgtaggac aggtccacac ggcgcagcaa
      181 ctgggcattg agcgccacca cgatggttga caggctcatg gcgatcgccg cggcggccgg
      241 gttgagcgtg aagccgatgc cggagaacac gccggccgca acgggtattg ccagcgcgtt
      301 gtacgccgtc gcccacacga gattctgaat catcttgcgg tgggaggcgc gggacagcgc
      361 gacgatagcg agcacgccgc gtggatcgct cgacgcgagc acgactccgg cggcttccat
      421 cgccacatcg gtgccggccc caatcgcgat gccgacgttg gcgcgggcga gcgcgggtgc
      481 gtcgttaacg ccgtcgccca ccatcgcgac cttccccccg cccgcctgca gcttctccac
      541 ggcggagtcc ttgtcttgag gcaacacctc cgcgaagacc tcgtcgatgc cgagttcacg
      601 cgcgacgcgg tcagcgaccg cctgggagtc tccggtgatg agagccaccg acacgccttg
      661 gcggtgcagc gcgtcgaccg ttgcccgcga cgcggcccgc acggggtcgt gcgtcgtgaa
      721 gagccccacg acggtaccgc cgacggtgac atacagcgtg gggcttccct cgccgtccca
      781 gcgttcgcgg gtttcgaccg cggccgccgg ggactcgata tccaattccg cgagcatcga
      841 ccggccgccg acgcccacgc gatcgccgtc gacggtgccg acgacgccct tgccgggaag
      901 cgtgtcgacg tccgacgcgt cgggcacggt gatgtcgcgt tcggcggccg ccgcgacgat
      961 ggcacgccca atggggtgct cgctgtcgcg ctccacggcg gccgcgacgg cgagcacgcg
     1021 ctcctcgtcc cagtcgtcga gcggctccac ctcggccacc gtgtggctgc cctcggtgag
     1081 agtgccggtc ttgtcgaaga gcaccgatga gatggaacgc atggtctcga gcgccaggac
     1141 atccttgacg agcacgccgg cttgggcgga ctttgcggtg gatatggcga tgaccagggg
     1201 aatggccagc cccagcgcgt gggggcatgc gatcaccagc acggtcacgg cgcgcacgat
     1261 gccggtcgag aagtctccct gcactgcgta gaccgtgaac gtcaccaggg cggaaccgag
     1321 tgcgatgtag aacaacgcct gcgcggcgcg atcggcgagc ttgcgtgccc cggaccgcga
     1381 ctcctgcgcc tgggtcacca tgcgctggat ccctgccaac gccgtgtcgt cacccactgc
     1441 ggtcacttcg acacgcagcg agccgtcagt cgcgactgtg cccgcgacga cgcggcctcc
     1501 ctccgcgcgt ctgacgggcg ccgactcgcc cgtgatcgtc gattcgtcca tgctggcgga
     1561 accttgagcg atcgtgccgt cggcgggaac gcgggcaccc ggtcggacca cgaccacgtc
     1621 gccggtgacg aggtccgcga tgcgcacggt ctcgacgtcg tcgcccacca cgcgttccgc
     1681 ctcgtcggga agcagctcag ccagcgcggc gagcgcgccg ctggcctgcc ccacggcctt
     1741 catctcctgc cagtggccca ggagcatcac cacgatgagc gtggcgagct cccaccagaa
     1801 gtcgaggctc ccgacgccga aggtgctcgc gagagacgcg aggaaagcga ccgtgatggc
     1861 gagtgcgatg agcagcatca tgcccggttg gcggttgcgg atctcgggta cggccatggt
     1921 gaagaacggg cgtccacccc agatgaagat gacggtgccg accaccggcg gaatccatga
     1981 cgatccgggg aaactcaggc tcaggccgag ccagtcctgc accatggccg agaagagcac
     2041 caccgggatg gcgagcacga ggttgatcca gaacagccgg cggaacaggt ccgcatggcc
     2101 gctgtggtcc gcgtgggcac tgtggtcggt gtgatcggtg tgggcaccct ggtccgcatg
     2161 cccgccgtgg tccggttccg cgccgtgggc ctcgttgctc gcgtgcgcgc cgtggtttgc
     2221 gtggtccatg tgatcgtctt ccgtcatgat tctcaggcta gaccgtcctt ctcactgaaa
     2281 ggtcaagagg ctacttgccg ccgaagggtc gggacctaag gccctgacca tcgcccctgg
     2341 acagaggaac acttcgatgg tgatcgaaat gacaatgccc cgctgggcgc ccgcaagtga
     2401 gtccgaggcc gtggacctcc tggcggcagc cacagagccc actcgctggc ggattctcgc
     2461 cgccctcgcc tcggcgggca cccggtgtg
//
LOCUS       DASRKH010000096         2283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_23524, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000096 DASRKH010000000
VERSION     DASRKH010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23524"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="VFC06_01610"
     CDS             complement(<1..605)
                     /locus_tag="VFC06_01610"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZL80631.1"
                     /translation="MILTIVGCSGSVAGPESAASCYLLEANDGARPWRVVFDLGSGAV
                     GPLQRYVELGAIDAVLLSHSHPDHAADAQALSVALRYGYPDPLRDALPLYGPAGIGEV
                     LSELDAASAFVVTELHPTPVQTVSLAIGPLTITVAPAWHPVPALAFRVDGPAEDGGRA
                     TFTYTGDTDRTEPIDALAEGVDLLLAEAGWAQGDTPEGRDGA"
     gene            complement(602..1408)
                     /gene="murI"
                     /locus_tag="VFC06_01615"
     CDS             complement(602..1408)
                     /gene="murI"
                     /locus_tag="VFC06_01615"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HZL80632.1"
                     /translation="MSDAPIGVFDSGVGGLTVARAIMDMMPHESIHYVGDTAHGPYGP
                     LPISEVREHALTIMDALVEDGVKLLVIACNTASAAVLRDARERFSRERGVPVVEVIVP
                     AVRRAASVTRSGRVGVIGTVATITSGAYDDALAAAPGVQVTSQACPRFVSFVEQGITS
                     GPEVIDVAREYLAPLQAAEVDTLILGCTHYPMLQGALSYVMGEQVTLVDSATETAVDV
                     YRALVAAGLERTSTEPPEHRFYATGPTESFEVLAQRFLGPIVRAVEPLRT"
     gene            complement(1401..2024)
                     /locus_tag="VFC06_01620"
     CDS             complement(1401..2024)
                     /locus_tag="VFC06_01620"
                     /inference="COORDINATES: protein motif:HMM:NF020989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2017 family protein"
                     /protein_id="HZL80633.1"
                     /translation="MRGFEARGDAAVAELDSEERAVVARVIADVGLLLDGEPFGMEPE
                     RVEDSEEELFRHLRGFEAALSDPEDPAVLRILPNAAPTDREVADEFRRLTEPELRATK
                     VDRLRVMWLVLNEDGPEWEVPEDQAMATAAALTDVRLVLAARLGVDTDDDADALHREL
                     EIAEHALATGGASDPVLNPERVWLGMLYQALTWLQESLMSYVMRDDV"
     gene            complement(2021..>2283)
                     /gene="clpS"
                     /locus_tag="VFC06_01625"
     CDS             complement(2021..>2283)
                     /gene="clpS"
                     /locus_tag="VFC06_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020013279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HZL80634.1"
                     /translation="DVVVESRLDDAWVTIVWNDPVNLMSYVTHVFQSYFGWPRDRAEA
                     WMREVHTAGKTVVSSGGREKMEIDVQAMHSFGLWATMQRSGQ"
BASE COUNT          384 a          800 c          753 g          346 t
ORIGIN      
        1 gcgccatccc tcccttcggg agtgtcgcct tgagcccagc ccgcttccgc aagcagcaag
       61 tccacacctt cggccagcgc gtcgatcggc tcggtccggt cggtgtcccc tgtgtacgtg
      121 aaggtggcgc gtccgccgtc ctccgccggc ccatcgacgc ggaacgcgag tgctggcacg
      181 ggatgccatg cgggagccac ggtgatggtg agcggaccga tcgcaagcga cactgtctga
      241 acaggcgtcg gatgaagctc cgtcacgacg aaggcgctcg ctgcatccag ctcgctcaac
      301 acctcgccga ttcccgccgg cccgtacaac gggagggcgt cccgcaacgg atccgggtag
      361 ccgtagcgca gcgccacgga cagcgcctga gcgtcggccg cgtggtcggg atgtgaatgg
      421 ctcagcagga ccgcatcgat cgcgcccaac tccacgtagc gctgcaaggg gccgacggcg
      481 ccggagccga gatcgaagac gacgcgccac ggtcgcgcgc catcattcgc ctcgagcagg
      541 tagcaggacg ccgcggactc cggcccggcg acggaccccg agcaacccac gatggtgaga
      601 atcatgtcct gagcggttcc accgcgcgga cgatgggccc gaggaagcgt tgggcgagca
      661 cctcgaacga ctcggtggga cccgtcgcgt agaagcgatg ctcggggggc tcagtcgatg
      721 tgcgttcgag ccccgcggcg acgagcgcgc ggtaaacatc caccgcggtc tccgtcgcgg
      781 agtcgacgag cgtcacctgc tcccccatga cgtaggacag ggcgccctgc agcatcggat
      841 agtgcgtgca gcccaggatg agggtgtcga cttctgccgc ctgcaacgga gccagatact
      901 cgcgggccac gtcgatgacc tcgggaccgg acgtgatccc ctgctccacg aaggacacga
      961 aacgggggca cgcctgtgag gtcacctgta cgcccggagc ggcggcgagt gcgtcgtcgt
     1021 aggcgccgga cgtgatggtc gccaccgtgc cgatgacgcc gacgcgaccc gacctggtca
     1081 cgctcgcggc gcgtctcacg gcgggaacaa tgacctcgac caccggcacg ccgcgctcgc
     1141 gggagaaacg ctccctggcg tcgcggagca ccgcagcgga cgccgtgttg cacgcgatga
     1201 ccagcagctt caccccgtcc tcgacgagcg catccatgat ggtgagggcg tgctcgcgca
     1261 cctcggagat gggcaagggc ccgtacggtc cgtgagcggt gtcgcccacg tagtgaatcg
     1321 actcgtgcgg catcatgtcc atgatcgcgc gggcgacggt gagtccgccc acgcctgagt
     1381 cgaagacacc gatcggcgcg tcagacatcg tccctcatca cgtagctcat cagcgattcc
     1441 tgaagccagg tcagcgcctg gtaaagcatg cccagccaca ccctctccgg attgaggaca
     1501 gggtctgagg cgcctcccgt cgcgagcgca tgctcagcga tttcgagttc ccgatgcagc
     1561 gcgtccgcgt catcgtccgt gtcgacgccg agccgggcgg cgagaaccag ccgcacgtcg
     1621 gtcagcgccg ccgccgtggc catggcctgg tcctcgggca cctcccattc gggcccgtcc
     1681 tcgttcaaga ccaaccacat cacccgcagg cgatccacct tggtggcgcg cagttcgggc
     1741 tcggtaagcc ggcggaactc gtcggcgact tcgcggtcgg tgggagcggc gttgggaagg
     1801 atccgcagca ctgcggggtc ttcggggtcg ctgagcgccg cttcgaagcc gcgcaaatgc
     1861 ctgaacagct cctcctcgga gtcctcgaca cgctccggct ccatgccgaa gggctccccg
     1921 tcgagcaaga ggccgacgtc ggcaatcact cgcgcgacca cggcgcgttc ctcggagtcc
     1981 agttctgcca cggcggcgtc accgcgggct tcaaaccctc tcactggccg ctccgctgca
     2041 tcgtggccca caggccgaag gagtgcatcg cctgcacgtc gatctccatc ttttcgcgac
     2101 cgccggagga cacgaccgtc ttgcccgcgg tgtgcacctc gcgcatccac gcctccgctc
     2161 ggtcacgcgg ccacccgaag tagctctgga atacgtgggt cacgtaggac atcaggttga
     2221 ccgggtcgtt ccacacgatc gtcacccagg catcgtcgag tcgggattcg acgacgacgt
     2281 cgg
//
LOCUS       DASRKH010000097         3477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_4053, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000097 DASRKH010000000
VERSION     DASRKH010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4053"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VFC06_01630"
     CDS             <1..382
                     /locus_tag="VFC06_01630"
                     /inference="COORDINATES: protein motif:HMM:NF015559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HZL80635.1"
                     /translation="GIEALSRGAAQATFVESATPAVRVIEANVKELGLRARATVVRDR
                     ALPYLKRVAAQWTIAFLDPPYDIAEADLAPVLEALVTRMAPGATVVLEMSSRRGTPEW
                     SRGLQVVQSKAYGDTTVYFAECVR"
     gene            393..875
                     /gene="coaD"
                     /locus_tag="VFC06_01635"
     CDS             393..875
                     /gene="coaD"
                     /locus_tag="VFC06_01635"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HZL80636.1"
                     /translation="MTTAVFPGSFDPITLGHVDIAVRARQLFDRVVIAVAHNSTKSPL
                     LDVDVRVRLAQLAVSHVDGIEVAATDGLLVDFCEAHGAVAIVKGLRGGADYDVERPMA
                     LMNRSLSGVETVFLTGDSSLSHIASSLIRDVARHGGDISEYVPPGVESAVLEALRSEN
                     "
     gene            970..1551
                     /locus_tag="VFC06_01640"
     CDS             970..1551
                     /locus_tag="VFC06_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006215061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HZL80637.1"
                     /translation="MEERVKHTPKADTEFRIHVRDLVRRPGTHREYSRDLTVVDALGT
                     EVIGVPVGAAATLKVQCEVVTDGIWVGGTFAAQATGECGRCLDEVKVDLEVPVQGLFL
                     YPDAPREEGDDADDVFEFDGDAIDCEGVVRDAVVTSLPFTPLCEPDCPGLCDQCGARL
                     ANDPDHAHEILDPRWSALESVKDSIELSDKEES"
     gene            1554..1748
                     /gene="rpmF"
                     /locus_tag="VFC06_01645"
     CDS             1554..1748
                     /gene="rpmF"
                     /locus_tag="VFC06_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HZL80638.1"
                     /translation="MAVPKRKMSRSNTRSRRSQWKTTAANLTTCPSCKGDKLSHAACP
                     ACGAYNGRQYAEALRTEHEA"
     gene            1770..2465
                     /gene="rnc"
                     /locus_tag="VFC06_01650"
     CDS             1770..2465
                     /gene="rnc"
                     /locus_tag="VFC06_01650"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HZL80639.1"
                     /translation="MSAPGPQAAAILTERLGVSIDPELLVLSLTHRSFAYENGGLPTN
                     ERLEFLGDSVLSIAVTDRLYRDHPDLPEGDLAKIRASCVSQRALAIVAREIELGSFVL
                     LGKGELSTGGRDKDSILCDTLEAIFGAVYLTHGIEVSREVILRLVGPSLIQASTAGDA
                     LDWKTALQEACSATGLPGPVYEVTGVGPDHARTFTATVLVDGIARGTGTGSSKKVAEQ
                     EAAEAARGALSAT"
     gene            2465..3406
                     /gene="mutM"
                     /locus_tag="VFC06_01655"
     CDS             2465..3406
                     /gene="mutM"
                     /locus_tag="VFC06_01655"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HZL80640.1"
                     /translation="MPELPEVETVRSGLADLVVGSTVTGVQVFRPSCVRLQPGGEGEF
                     AMCLQGMVLDAVVRRGKFLWIPLAEAEDAGGERTPAKALSAHLGMSGQFRVFEGDPPE
                     PHSHCRARLHLESPDGPRTVDFLDQRTFGYLVAEQMQPTPDGLAGGHGSALPAVPNTV
                     AHIARDALDPGAVDAHIVSRMRRGSRGIKQVLLDQTVVSGVGNIYADEALWAARIHPQ
                     RPAQAVSGPQARALLAAVRTVMTRALAHGGTSFDALYVDVNGESGYFSRELAAYGRAG
                     QPCERCGGLISREHIGGRSTHWCPRCQRRYGPQASRD"
BASE COUNT          557 a         1137 c         1171 g          612 t
ORIGIN      
        1 cggaatcgag gcgctgagca ggggagcggc ccaggcgacg ttcgtggagt cagccacgcc
       61 cgccgtgcgc gtcatcgagg cgaacgtcaa ggagctcgga ctccgggccc gcgccaccgt
      121 ggtgcgcgat cgggcgctgc cgtatctgaa gcgtgtggcg gcgcagtgga cgatcgcgtt
      181 tctcgacccg ccctacgaca tcgccgaggc ggacctggcg cccgtcctcg aagcgctcgt
      241 gacgcgcatg gcgccggggg ccaccgtcgt gctggagatg tcgtcccgcc gcggcacccc
      301 ggagtggtcg cgcggactgc aggtggtcca aagcaaggcg tatggcgaca ccacggtcta
      361 ctttgccgag tgcgtgcgat aagttgccga tcatgaccac agccgtcttt ccaggatcct
      421 ttgacccgat caccttgggt cacgtcgaca tcgccgtgcg cgcacggcaa ttgtttgacc
      481 gagtggtgat cgcggtggcg cacaactcca ccaagtcgcc gttgctggac gttgacgttc
      541 gcgtgcgcct ggcccaattg gcggtttctc acgtcgatgg catcgaggtc gccgcgacgg
      601 acgggctgct ggtggacttc tgcgaggcgc acggagcggt ggcgatcgtc aagggcttgc
      661 gtggcggcgc cgactacgac gtcgaacgcc ccatggcgct gatgaaccgc tcgctgagcg
      721 gcgtcgagac cgtcttcctc acgggagaca gctccctgtc acacatcgcc tcctcgctca
      781 ttcgagacgt cgcaaggcac ggcggagaca tctccgaata cgtgccgccg ggcgtcgagt
      841 cggccgtatt ggaagcgctc cgcagcgaga actagcggcg ccgcttgagt tcacggcctg
      901 cgatcgcgta gtatcgtctg ctggcccgcc ggtcactacc gggccgaggt cccgaaggac
      961 cgttaggaca tggaggagcg tgtgaaacac actcccaaag ccgacaccga atttcgcatt
     1021 cacgtgcgcg acttggtgcg caggccaggt actcatcgcg agtactcccg tgatctgacg
     1081 gttgtcgatg cccttggcac tgaagtgatt ggggttcctg tgggagccgc cgcgacactg
     1141 aaggttcagt gcgaggtggt gactgacgga atctgggtcg gcggcacctt cgcagcgcaa
     1201 gcgacggggg agtgcggtcg ctgtctggat gaggtgaagg ttgacctcga ggttccagtg
     1261 cagggtctgt ttctctaccc tgatgccccg cgtgaagagg gtgacgacgc ggacgacgtg
     1321 ttcgaattcg acggcgacgc catcgactgc gaaggcgtgg tcagggatgc ggtggtcacc
     1381 agtctgccgt ttacccccct gtgtgagccg gattgtccgg ggctgtgcga ccaatgtggc
     1441 gctcggctcg cgaacgatcc ggaccacgcg catgagatac tcgaccctcg ttggtcggca
     1501 ctagaaagtg tgaaagacag catcgagctg tctgacaagg aagagagtta gctgtggctg
     1561 ttcccaagcg caagatgtcg cgcagcaata cccgctcgcg tcgttcgcag tggaagacaa
     1621 ctgctgccaa cctcacgacg tgcccttcgt gcaagggcga caagttgtcg cacgcggcct
     1681 gcccggcctg cggcgcgtac aacggtcgcc agtatgccga ggcgttgcgc accgagcacg
     1741 aggcttaagg tctcttctcg ggtttacccg tgagcgctcc tggcccccaa gcggcggcca
     1801 tcctgactga gcggctcggt gtctcaatcg accccgagct gcttgtcctg tcgctcaccc
     1861 accggtcttt cgcgtacgaa aacggcggac tgcccacgaa cgagcgcctc gaatttcttg
     1921 gcgactccgt gttgagcatc gccgtgacgg accgtttgta tcgcgaccat cccgacctcc
     1981 ccgagggtga cctcgcgaag attcgtgcgt cgtgtgtttc ccagcgtgcc ctcgccatcg
     2041 tcgcccgcga gatcgagctg ggctcctttg tgttgctcgg caagggcgaa ctgtcgaccg
     2101 ggggccgcga caaggattcg atcctgtgcg acaccctcga agccatcttc ggcgcggtct
     2161 acctgaccca cgggatcgag gtgtcgcgtg aggtcatcct gaggctcgtc gggccctccc
     2221 tgatccaggc ctccacggcg ggcgatgcct tggactggaa gaccgctctt caagaggcct
     2281 gctccgccac gggtcttccc ggaccggtct acgaggtgac aggcgtggga ccggaccacg
     2341 ctcgcacctt caccgccacc gtcctcgtcg acggcattgc ccgtggcacg ggcacgggct
     2401 cgtccaagaa ggtcgccgaa caagaggccg ccgaggctgc ccgcggcgcc ctgtccgcga
     2461 cgtagtgccc gaacttcccg aggtcgagac ggtccggtca gggctggcgg atctggtggt
     2521 gggatccacg gtcacgggcg tccaggtctt tcgcccatcg tgcgtccgcc tccaaccggg
     2581 tggcgaaggc gagttcgcga tgtgcctcca gggcatggtg cttgacgccg tcgtgcgtcg
     2641 cgggaaattc ctgtggatcc cgctcgccga ggcggaggac gcgggcggcg agcgcacccc
     2701 cgcgaaggcg ctcagcgccc acttgggcat gtcgggacag ttccgcgtct tcgagggcga
     2761 ccctcccgag ccccacagcc actgtcgggc acgactgcac cttgagtctc ccgacggccc
     2821 tcgcaccgtg gacttcctgg accaacgcac gttcggatac ctggtggccg aacagatgca
     2881 gcccacgccc gacgggctcg cagggggcca cggcagtgca ctgcccgccg ttcccaacac
     2941 ggtggcgcac atcgccaggg acgccctcga cccgggggca gttgacgctc acatcgtttc
     3001 ccgcatgcga cgaggatccc gcggcatcaa gcaggtgctc ctggaccaga cggtggtgag
     3061 cggagtcggc aacatctatg ccgacgaggc gctgtgggcg gcccgcatcc acccccagcg
     3121 tccggcccaa gcggtgtcgg gtccccaggc tcgcgccctc ctggcggcgg tacgaacggt
     3181 gatgacgcga gcgctcgcgc acggtggcac cagttttgac gcgttgtacg tcgacgtcaa
     3241 cggcgagtcg ggatattttt cgcgggagtt ggccgcctat gggcgtgcgg ggcagccgtg
     3301 cgagcgttgc ggcggactga tctcgcgtga gcacatcggc ggccgttcga cgcactggtg
     3361 tccccgctgc cagcgcaggt acggacctca ggcctcccgg gactaggact ccccggacgt
     3421 ctgtcccggc gacgcgcccg ggactccgaa gcggccgccg cacggttgcc tgtagga
//
LOCUS       DASRKH010000098         3179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_5198, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000098 DASRKH010000000
VERSION     DASRKH010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5198"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..468)
                     /locus_tag="VFC06_01660"
     CDS             complement(19..468)
                     /locus_tag="VFC06_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005270005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HZL80641.1"
                     /translation="MPINVEYAGREYPPQAAYEVSREKIREFAVAVGATHPAHTVPSA
                     ARALGYPDVIATPTFAVLVAQRAEAQLIEDPAAGIDFSRVVHAEERFVHHRPIHAGDR
                     LVTVLHVDSVVERAGLAMVTTRAEIADDAGTPVATVISTLAVRGVDE"
     gene            complement(484..654)
                     /gene="rpmG"
                     /locus_tag="VFC06_01665"
     CDS             complement(484..654)
                     /gene="rpmG"
                     /locus_tag="VFC06_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HZL80642.1"
                     /translation="MASKTADVRPKITLACVECKERNYITKKNRRNDPDRLDMKKFCP
                     RCGKHTSHRETR"
     gene            complement(733..809)
                     /locus_tag="VFC06_01670"
     tRNA            complement(733..809)
                     /locus_tag="VFC06_01670"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(773..775),aa:Met,seq:cat)
     gene            complement(875..947)
                     /locus_tag="VFC06_01675"
     tRNA            complement(875..947)
                     /locus_tag="VFC06_01675"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(912..914),aa:Thr,seq:ggt)
     gene            1178..1381
                     /locus_tag="VFC06_01680"
     CDS             1178..1381
                     /locus_tag="VFC06_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80643.1"
                     /translation="MPIDDALRMEITDADVRAAKKDWLAARDGVDPDRDVERAFWYYT
                     RLISTQAQQIADEVRKPGYRRAT"
     gene            complement(1674..1755)
                     /locus_tag="VFC06_01685"
     tRNA            complement(1674..1755)
                     /locus_tag="VFC06_01685"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1719..1721),aa:Tyr,seq:gta)
     gene            1914..2411
                     /locus_tag="VFC06_01690"
     CDS             1914..2411
                     /locus_tag="VFC06_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006887761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HZL80644.1"
                     /translation="MASEASFDVVSKVDRQEVDNALNQAVKEVAQRYDFKNIGASIGW
                     SGEKILMVANSEERVLAILDVLQTKLIRRGISLKSLDTGDGKPRASGKEYRLEASLKE
                     GLSSEVAKKIGKLVRDEGPKGVKSTITGDEVRISAKSRDDLQEVIALLKGADLDAALQ
                     FTNYR"
     gene            complement(2408..>3179)
                     /locus_tag="VFC06_01695"
     CDS             complement(2408..>3179)
                     /locus_tag="VFC06_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZL80645.1"
                     /translation="HGAEVRQHVRVTGIEVAADGGAVVHVVDQAPHSEARVTTLRASA
                     VVVTAGAWTAGLLAGVPGFDALPPLVVTQEQPAHFPVRDTALVADDGWPSFIHHVDPA
                     AHAEARLPDAPDGGYPVAGVYGHHTPGEGVKVGHHGTGPVVDPDAREPLPHVRAELAE
                     PARLGGEVPGVGVDAPAPISCTYTTTPDERFLLDRVGPVVIGAGFSGHGFKFTPGIGQ
                     VLADLATDVRPEVPGVDLGAFALARFTADAPAPEAVEV"
BASE COUNT          494 a         1110 c         1114 g          461 t
ORIGIN      
        1 gctcggggcg agcggccgtc attcgtccac cccgcggacc gcgagcgtcg agatcacggt
       61 cgcgaccggg gtgccggcgt cgtcggcgat ctcggcacgg gtcgtgacca tcgctaggcc
      121 ggcgcgttcg acgaccgagt cgacgtgcag aacggtcacc aagcgatccc ccgcgtggat
      181 cggacggtgg tggacgaagc gctcctcagc gtgcacgacg cggctgaagt cgatccccgc
      241 agcggggtcc tcgatcagct gggcctcggc ccgctgggcg acgaggaccg cgaaggtcgg
      301 cgtcgcgatg acgtccgggt agcccagggc ccgggccgcc gacgggacgg tgtgcgccgg
      361 atgggtcgct cctaccgcaa ccgcgaactc ccggatcttc tctcgggaga cctcgtaggc
      421 cgcctggggt gggtactcgc ggccggcgta ctcgacgttg atcggcacgg gagcggcgtg
      481 gcgtcagcgg gtctcgcggt gcgacgtgtg cttgccgcaa cgcgggcaga acttcttcat
      541 gtcgaggcgg tcggggtcgt tgcgccggtt cttcttggtg atgtagttcc gttccttgca
      601 ctccacgcag gcaagcgtga tcttcggtcg gacgtctgcg gtcttgctgg ccacggttgc
      661 tcacctctcg tgccgctcgc gcggtacttg cttctctgca cacgccggcc ggcgcgtggt
      721 cgtgttcctt ggtggtagcg ggggcgggac tcgaacccgc gacaccacga ttatgagccg
      781 tgtgctctaa ccacctgagc taccccgcct tgtatcgatg tgcggaggcc gctccacgga
      841 cgagccgtga cacgaacccc cgacacacca acagagcccc gaaagggaat cgaacccttg
      901 accttctcct taccatggag acgctctacc gactgagcta tcggggcagc gcgagcaaga
      961 ttacacggcc gcaggcgtgg ccgcgaaatc cggccccgcg gccggcccgg acgcgctcct
     1021 gtcggcctgt ttccggcgcc gtcgccgggt cgaccagccg catcgtgaga cgcgccggac
     1081 gccaggtggg tgacgttcgc gctgtccgct gcaccagccg ggtgaactcg tcgccccacg
     1141 ctgtgaactg gacgtctgta tcggggagag tacgcgggtg cccatcgacg atgcgctccg
     1201 catggagatc acagatgccg acgtgcgcgc ggccaagaag gactggctcg ccgcacgtga
     1261 cggggtcgac cccgaccgcg acgtcgagcg agccttctgg tactacacgc gcctcatctc
     1321 gacccaggcg cagcagatcg ccgacgaggt ccgcaagccc ggctaccgcc gcgcgacgtg
     1381 agcagggcct gagaaccgcc tgaggtctgg cgccctcgtc gtcctgctcg ccgtcgcgga
     1441 caccaaggac ggcgtcgacg ccgagctcca gagcgcggtc gaccagctca aggccgccgg
     1501 cgcgttcgac accctgtacg acggagccgt cgtctcggtc acgcccggaa ccaacgcctg
     1561 cgcgacctgg cctgcggtct cccggacgct cccggaggct cccggaggct cccggaggct
     1621 cccagacagc gaacggggcg ccctctcatc gagaggacgc cccgttcgtt ctgtggcggg
     1681 tgagggattc gaacccccgt aggcgttgcc agctgattta cagtcagccc cctttggcca
     1741 ctcgggtaac ccgccagggt tgtacgcagg tgtcacttca cagtcgcaac gccgcgcacg
     1801 gcgtgcgggt ggaaggatag caactccacc ggggtgttcg cgcagcatcc cgacacgcag
     1861 acgttcagcg tcacgactcg acccgccgcg tgcggctcga cagggaggac accatggcga
     1921 gcgaagcgtc gttcgacgtc gtcagcaagg tcgaccggca ggaggtcgac aacgcgctca
     1981 accaggccgt caaggaggtc gcgcagcggt acgacttcaa gaacatcggg gcgtccatcg
     2041 gctggagcgg cgagaagatc ctcatggtcg cgaactccga ggagcgcgtg ctcgcgatcc
     2101 tcgacgtgct gcagaccaag ctcatccggc gtgggatctc gctgaagtcg ctcgacacgg
     2161 gcgacggcaa gccgcgcgcc tccggcaagg agtaccgcct cgaggcctcg ctcaaggagg
     2221 gcctgagcag cgaggtcgcc aagaagatcg gcaagctcgt gcgcgacgag ggccccaagg
     2281 gcgtcaagtc gaccatcacg ggcgacgagg tgcggatctc ggcgaagagc cgggacgacc
     2341 tccaggaggt catcgcgctg ctcaaggggg cggacctcga cgccgccctg cagttcacga
     2401 actaccgcta gacctcgacc gcctcgggag cgggcgcgtc ggctgtgaac cgggcgagcg
     2461 cgaacgcgcc gaggtccact cccggcacct cagggcgcac atcggtcgcc aggtcggcga
     2521 gcacctggcc gatgccgggc gtgaacttga agccgtgccc cgagaacccg gcaccgatca
     2581 cgaccggtcc gacgcggtcc agcaggaacc gctcgtcggg ggtcgtcgtg taggtgcagc
     2641 tgatcggtgc gggcgcgtcg accccgacgc ccgggacttc accgccgagc cgggccggct
     2701 cggcgagctc cgcgcgtacg tgcgggagtg gttcccgggc gtcggggtcg acgacggggc
     2761 cggtcccgtg gtgaccgacc ttcacgccct caccgggcgt gtggtggccg tacacgcccg
     2821 ccaccgggta gccgccgtcg ggcgcgtccg gcagccgggc ctcggcgtgc gcggccgggt
     2881 cgacgtggtg gatgaagctg ggccaaccgt cgtcggcgac cagtgccgtg tcacgaaccg
     2941 ggaagtgcgc aggctgctcc tgtgtcacga cgagcggcgg cagcgcgtcg aaccccggca
     3001 cgcccgcgag cagcccggcc gtccacgcgc cggccgtcac gacgaccgcg ctcgcccgga
     3061 gcgtggtcac gcgcgcctcg gagtgcggcg cctggtcgac gacgtgcacg acggctcccc
     3121 cgtcggcagc cacctcgatc cccgtcacac gcacgtgctg acgcacctcg gcaccgtgc
//
LOCUS       DASRKH010000099         2967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_5612, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000099 DASRKH010000000
VERSION     DASRKH010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5612"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            412..753
                     /locus_tag="VFC06_01700"
     CDS             412..753
                     /locus_tag="VFC06_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HZL80646.1"
                     /translation="MFDSVSSVALNSALDGLALRQRTIAENVANINTPGYQAKRVQFE
                     TALARAVEQGSGAATSTVARSLEPTRTDGNNVNLDTETTLNVDTNLRYQLATQALDQT
                     FSTVRIAMRTS"
     gene            753..1157
                     /locus_tag="VFC06_01705"
     CDS             753..1157
                     /locus_tag="VFC06_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HZL80647.1"
                     /translation="MSIFGAIGIASSGMTVNRKWLDAVSDNLANVSTVRRTSEAAYQA
                     KSVIVSEIPTANGGGVQVSGIALGDAVGRVVSDPTNPLADAQGYVRQPDIDMSSQMTQ
                     LIMAQRGYQANAAVVDRAKATYEAALQIGRSS"
     gene            1154..1477
                     /locus_tag="VFC06_01710"
     CDS             1154..1477
                     /locus_tag="VFC06_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HZL80648.1"
                     /translation="MSIPAVSALSAGISPVTPTAYLSSAGGAGGTTGASATGFASALG
                     SVDQLQQMQSTTDALAVKAVTGSLTDVHDYTIAAAESKLTLELTAAVRNKAVDAFNEI
                     MRMQA"
     gene            1477..>2967
                     /gene="fliF"
                     /locus_tag="VFC06_01715"
     CDS             1477..>2967
                     /gene="fliF"
                     /locus_tag="VFC06_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HZL80649.1"
                     /translation="MPTQVTAVWGRFASTLKQFTMAQRTLAVIGLAVLLLGGFAVTSW
                     VSKPTMSPLFTSLSATDASAVVDQLTSEGVAYQLADGGSTVLVPNDKLYAMRLKLAAA
                     GLPANADGGGYSLLDSMGMTSSEFQQQVTYQRALEGELAKTVGALQGVDAATVKLAIP
                     QDTVFVATKADPTASVFIRTKAGVTLSTDQVQAIVHLVSAGIENMKPTDVAVIDSTGK
                     VLSTVGGELMSGSDGQQTGDYEARVSAAVQVLLDQVVGVGKSAVTMNAVLDYDKTQRT
                     SESFTAAPNTPALASSKKSESYTGNGSTATGVLGPDNIAVPSGNSTAGTYASASEDVT
                     NAVDKVTELTTTAPGSVRRQSLAVAIDAKAAAAIDMTQLTAMLSAAAGIDATRGDTIA
                     VQALAFDTSAAQTAQDALAAADTQSAKAASASLVRQGAIAGVILLVVLALVIAGARRS
                     RRSRRESLDIGELSRLDRDVDPLGIGAGADGLPALPAGMAPVPAMPD"
BASE COUNT          483 a         1113 c          990 g          381 t
ORIGIN      
        1 ccccgcatcc ctgcagaccc gcgccgaggc gctcctcgac cgacagatca gcgtgggccg
       61 ccgcatcgcc gaggctgcca acctcagccg ccgccaggcc gtcgccgccg agggcatgcg
      121 cagccgcccg ccggccgtcc cggtgtacat cgacaccgag ggctgagccg gcccggggca
      181 cgcgtcgagg ggtgcagcgg gtgacgtgcc gaccggacag gtgcgacacc gtgggacaca
      241 cggtgacgta gtcgttcggc caggcgagtg aacgtcaaca tctgccgctc ggaacccctc
      301 acccacccag ccaggtgccc gatggggacg ccgagcacgg atcgctcacg cagcaggcca
      361 cggaccggcc gacccgagca ccatcgtcgg tgaggtggac gcttcatgtc cgtgttcgac
      421 tctgtgagct ccgtggcgct gaacagcgcc ctggacgggc tcgcgctgcg tcagcgcacc
      481 atcgcggaga acgtcgcgaa catcaacacc cccggctacc aggccaagcg cgtccagttc
      541 gagaccgctc tcgcccgcgc cgtcgagcag gggagcggcg cggcgacctc gaccgtcgcc
      601 cgctcgctcg agccgacgcg caccgacggc aacaacgtga acctcgacac cgagacgacc
      661 ctcaacgtcg acacgaacct gcgctaccag ctcgcgaccc aggcgctcga ccagacgttc
      721 tcgacggtcc gcatcgcgat gaggacgagc tgatgagcat cttcggggcg atcgggatcg
      781 ccagctcggg catgaccgtc aaccgcaagt ggctcgacgc ggtgagcgac aacctcgcga
      841 acgtctcgac cgtgcgccgg acgagcgagg ccgcgtacca ggccaagtcg gtgatcgtga
      901 gcgagatccc gaccgcgaac ggcggcgggg tccaggtctc cggcatcgcg ctcggcgacg
      961 ccgtcgggcg cgtcgtctcc gatccgacca acccgctcgc cgacgcgcag ggctacgtgc
     1021 gtcaacccga catcgacatg tcgtcccaga tgacccagct gatcatggct cagcgcggct
     1081 accaggcgaa cgccgccgtg gtcgaccgcg ccaaggccac ctacgaggca gccctccaga
     1141 tcggacgttc ctcatgagca tccctgccgt gtccgcgctc agcgcgggca tctcgccggt
     1201 caccccgacc gcctacctca gctccgcggg cggcgccggt ggcacgacgg gcgcgtctgc
     1261 gaccggcttc gcctcggccc tcgggtcggt cgaccagctc cagcagatgc agtcgacgac
     1321 cgacgcgctc gccgtcaagg ccgtcacggg cagcctcacc gacgtgcacg actacacgat
     1381 cgcggccgcc gagtcgaagc tcacgctcga gctgaccgcg gcggtccgta acaaggccgt
     1441 cgacgcgttc aacgagatca tgcggatgca ggcctgatgc ccacccaggt cacggccgtg
     1501 tggggccgct tcgcgtcaac gctcaagcag ttcacgatgg cgcagcgcac gctcgcagtc
     1561 atcgggctcg cggtcctcct gctcggcggg ttcgcggtga cgagctgggt gtcgaagccg
     1621 accatgagcc cgttgttcac gagcctgtcc gcgaccgacg cgagcgcggt cgtcgaccag
     1681 ctcacgtccg agggcgtcgc ctaccagctc gcggacggcg gctccacggt cctggtcccc
     1741 aacgacaagc tctacgcgat gcggctcaag ctcgcggccg cggggctacc cgcgaacgcc
     1801 gacggcggcg gctactcgct cctcgacagc atgggcatga cgtcgtcgga gttccagcag
     1861 caggtcacct accagcgcgc gctcgagggc gagctcgcca agacggtcgg tgccctgcag
     1921 ggcgtcgacg cggcgaccgt caagctcgcg atcccgcagg acaccgtgtt cgtcgcgacc
     1981 aaggcggacc cgaccgcgtc ggtcttcatc cgcaccaagg ccggcgtgac gctgagcacc
     2041 gaccaggtcc aggcgatcgt gcacctcgtc tcggcgggca tcgagaacat gaagcccacg
     2101 gacgtggccg tcatcgactc gaccggcaag gtcctgtcga ccgtcggtgg cgagctcatg
     2161 tcgggttcgg acggtcagca gaccggcgac tacgaggctc gggtcagcgc agcggtccag
     2221 gtgctcctcg accaggtcgt cggcgtcggc aagtccgccg tcaccatgaa cgcggtcctc
     2281 gactacgaca agacgcagcg gacgagcgag tcgttcaccg cggcgcccaa caccccggcg
     2341 ctcgcgtcgt cgaagaagag cgagtcgtac acgggcaacg ggtcgaccgc gacgggcgtg
     2401 ctcggaccgg acaacatcgc ggtgccgagc gggaactcga cggccggcac gtacgcgtcg
     2461 gccagcgagg acgtcaccaa cgcggtcgac aaggtcaccg agctcacgac gaccgcgccg
     2521 ggcagcgtcc ggcgccagtc gctcgcggtc gcgatcgacg cgaaggccgc agcggccatc
     2581 gacatgaccc agctgaccgc gatgctcagc gccgccgcgg gcatcgacgc cacgcgcggc
     2641 gacaccatcg cggtgcaggc cctcgcgttc gacacgtccg cggcgcagac cgcccaggac
     2701 gcgctcgccg cggccgacac gcagtcggcg aaggctgcgt ccgcctcgct cgtccgccag
     2761 ggcgcgatcg ccggtgtgat cctcctcgtc gtcctcgcgc tcgtcatcgc cggggctcgt
     2821 cgctcgcgcc gcagccgccg cgagtcgctc gacatcggtg agctgtcgcg gctcgaccgt
     2881 gacgtcgacc cgctcgggat cggcgccggc gccgacggac tgcccgcgct gcccgccggg
     2941 atggcacccg tgcctgccat gcccgac
//
LOCUS       DASRKH010000100         2215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_3718, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000100 DASRKH010000000
VERSION     DASRKH010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3718"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VFC06_01720"
     CDS             complement(<1..206)
                     /locus_tag="VFC06_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HZL80650.1"
                     /translation="MPELPEVETVRDGLARHVLDRTVTAVEVRREYSVRRHEGGPLDF
                     AGRLTGRRFEAAVRRGKFLWLPLDP"
     gene            complement(246..938)
                     /gene="rnc"
                     /locus_tag="VFC06_01725"
     CDS             complement(246..938)
                     /gene="rnc"
                     /locus_tag="VFC06_01725"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HZL80651.1"
                     /translation="MSIASAQLVEKLGIHLDPELLVLALTHRSFAHEAGGIPTNERLE
                     FLGDTVLGLVVTEALYRDHPGQPEGVLAKMRAATVSQRGLAAVARDLDLGAYVLLGKG
                     ELSTGGPDKDSILSDTLEALFGAVYLAHGLEPARELVMSLVGPTLVAAAELGAGLDWK
                     TSLQELSAELGLGAPTYEVDGVGPDHARTFTARAVVGDEVRGSGTGAAKKLAEQHAAA
                     DAYRALDALRPA"
     gene            complement(1003..1200)
                     /gene="rpmF"
                     /locus_tag="VFC06_01730"
     CDS             complement(1003..1200)
                     /gene="rpmF"
                     /locus_tag="VFC06_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HZL80652.1"
                     /translation="MAVPKRKMSRSNTRARRSQWKTTATALTTCPQCKANKQPHMACP
                     TCGAYNNRQYAEAVRTEHEVG"
     gene            complement(1203..1796)
                     /locus_tag="VFC06_01735"
     CDS             complement(1203..1796)
                     /locus_tag="VFC06_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HZL80653.1"
                     /translation="MGTWKVQRAPHLDPRSPFVLDTHELGRRPGSMRTVQRTVKAPED
                     LGTVVIGVPAGTDLELDLRLEAVMEGVLVSGSVRGRAIGECIRCLDETVEDLDVTLQE
                     LYVYPERAAVAVEDGDDEEDVRKLDGDLIDVEPALRDAVVPALPFQPLCRPDCPGLCS
                     ECGAHLADDPDHSHEILDPRWAALGGLQSTYDETKES"
     gene            complement(1941..>2215)
                     /locus_tag="VFC06_01740"
     CDS             complement(1941..>2215)
                     /locus_tag="VFC06_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80654.1"
                     /translation="QSTSSEAVLVRARQRASELVDAEAVVVAARERAAEILRGAEVTA
                     EGLRRDADDYCDRRLADFEIDLGKLLSQVQGGRARLADRLGGPGEG"
BASE COUNT          315 a          793 c          789 g          318 t
ORIGIN      
        1 gggtccagcg ggagccacag gaacttgccg cgtcggaccg cggcctcgaa gcgtcgaccg
       61 gtcaggcgcc cggcgaagtc gagcgggccg ccctcgtggc gtcgcacgct gtactcgcgg
      121 cggacctcga ccgcggtcac cgtgcggtcc aggacgtgcc gggcgagccc gtcgcggacg
      181 gtctcgacct cgggaagctc aggcacggcg aggcacgctc acgcgatcgc tcggggcgct
      241 gccgctcagg ccggtcgcag cgcgtcgagc gcgcggtagg cgtccgccgc ggcgtgctgc
      301 tcggcgagct tcttggccgc gccggtgccg ctgccgcgca cctcgtcgcc gacgaccgcg
      361 cgggccgtga acgtgcgggc atggtcgggt cccacgccgt cgacctcgta ggtcggtgcg
      421 ccgagaccga gctcggccga gagctcctgg agactcgtct tccagtcgag gcctgcgccg
      481 agctctgccg cggccacgag ggtcgggccc acgagcgaca tgacgagctc gcgtgccggc
      541 tcgagaccgt gtgcgaggta gaccgcgccg aacagcgcct cgagcgtgtc agacaggatc
      601 gagtccttgt ccggaccgcc ggtcgacagc tcgcccttgc cgagcagcac gtacgcgccc
      661 aggtccaggt cgcgtgccac agcagcgagc ccacgctgcg agaccgtcgc ggcgcgcatc
      721 ttcgcgagca caccctcggg ctggccgggg tggtcgcggt agagcgcctc ggtgacgacc
      781 aggccgagca cggtgtcccc gaggaactcg agccgctcgt tcgtcgggat gccgccggcc
      841 tcgtgcgcga acgagcggtg ggtgagcgcc agcacgagaa gctcgggatc caggtggatc
      901 ccgagcttct cgacaagctg ggcggacgcg atgctcatgc cccggccacc acggaggtga
      961 tcggacgcag agcgacgatc gcgaccgctg ctgcgctgag cgtcagccga cctcgtgctc
     1021 ggtgcgcacg gcctcggcgt actggcggtt gttgtacgcg ccgcacgtcg ggcacgccat
     1081 gtgcggctgc ttgttggcct tgcactgcgg gcaggtggtg agcgcagtgg cagtggtctt
     1141 ccactgcgac cgacgcgcac gggtgttgct gcgcgacatc ttgcgcttcg gaacagccac
     1201 ggctagctct ctttcgtctc gtcgtacgtg ctctgcaggc caccgagggc cgcccaccga
     1261 gggtcaagga tctcatgcga atggtcgggg tcgtccgcga ggtgcgctcc acactcggag
     1321 cacagacccg ggcagtccgg ccggcacagc ggttggaacg gcagtgcagg caccacagcg
     1381 tcccggagcg cgggctcgac gtcgatcagg tcgccgtcca gcttgcgcac gtcctcctcg
     1441 tcgtcgccgt cctcgaccgc gacagccgcg cgctcagggt agacgtacag ctcctggagc
     1501 gtgacgtcga ggtcctcgac cgtctcgtcc aggcagcgga tgcactcccc gattgctcgg
     1561 ccgcggacgg atccggagac caggacccct tccatgaccg cctcgagccg aagatccagc
     1621 tccaggtcgg tcccggcagg gacgccgatc accacggtac cgagatcctc gggggccttg
     1681 accgtgcgct gcaccgtccg catcgatccc ggacgtcgac cgagctcatg ggtatcgagc
     1741 acgaagggcg aacggggatc gaggtgaggc gcgcgctgca ctttccaggt tcccatcgtc
     1801 aggttctcgg tcacgccacg accggcagga ccaacggtca agcctacggc actggaggcc
     1861 gcctgcccaa acggccgggc gctgaccggc ccttcgaccg gtctgggacc ggtctgaggc
     1921 cggctggtca gctcggtccg tcagccctcg cccggaccac cgagccggtc ggcgagcctg
     1981 gcacgcccgc cctgcacctg gctgagcagc ttgccgaggt cgatctcgaa gtcggcgagc
     2041 cgacggtcgc agtagtcgtc cgcgtcgcgg cgaaggccct cggccgtcac ctcggcgccg
     2101 cgcaggatct ccgcggcgcg ctcacgcgcc gcgacgacca cggcctcggc gtccacgagc
     2161 tccgaggccc gctggcgggc gcgaacgagc acggcctcgg agctcgtgga ctgtc
//
LOCUS       DASRKH010000101         2178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_19882, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000101 DASRKH010000000
VERSION     DASRKH010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19882"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VFC06_01745"
     CDS             <1..380
                     /locus_tag="VFC06_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011931544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZL80655.1"
                     /translation="CEQLTAVGNVRLVLDRATRASAVAEELRRVGLDGESLVKPVRDL
                     SGGQRRRVAIVRALMADADLIVLDEPFKGLDTEAKTLVMSYVRERCDHRTMLLITHDP
                     AEAEWFGIRPIELARRGTSEPSR"
     gene            507..1547
                     /locus_tag="VFC06_01750"
     CDS             507..1547
                     /locus_tag="VFC06_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZL80656.1"
                     /translation="MRYRTLGNSGAIVSTFALGTMTFGTEADEATSHAILDDYVAAGG
                     NFIDTADVYSAGVSEEIIGRWLAAHPAEREQVVIATKGRFPMGVGPNDLGTSRRHLTR
                     ALDASLRRLGTDQIDLYQMHAWDAVTPLEETLRFLDDAVRAGKIAYYGFSNFLGWQLT
                     KAVHVARASGFTPPVTLQPQYSLLVREIESEIVPAALDANIGLLPWSPLAGGWLTGKY
                     RRDADPTGATRLGENPERGMEAWEPRNKQERTWRILETVAEIATAQGVNQAQVSLAWL
                     EAQPAVTSVILGARTTAQLADNLGAASVVLTAGELGRLDEVSAPVVSDYPYGTAGAAQ
                     RHRANDGIRVLR"
     gene            1725..>2178
                     /locus_tag="VFC06_01755"
     CDS             1725..>2178
                     /locus_tag="VFC06_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80657.1"
                     /translation="MRSTPSPIRKRIAGVALVGAIAGAGVVAAAAAANAADSTPTATT
                     SSTAATDTPAAGGAVDESKSVRSDEHLLTGTDAASATAAALAKYPGATIQRVETDSAG
                     VYEAHLITAAGERLIVQMDATFTVTGNDTGGPAGGTRGADPTDAPSTTT"
BASE COUNT          355 a          834 c          716 g          273 t
ORIGIN      
        1 tgtgcgagca gctcaccgcg gtcggcaacg tgcgtctggt cctggatcga gccacccgtg
       61 cgtccgccgt cgcggaggag ctgcgccgcg tgggcctgga cggcgagtcc ctcgtgaagc
      121 cggtacgtga cctctcgggt gggcagcgtc gacgggtcgc gatcgtccgg gccctgatgg
      181 ccgacgcgga cctcatcgtc ctcgacgagc ccttcaaggg gctggacacc gaggcgaaga
      241 ccctggtgat gtcctatgtc cgcgagcgct gcgaccaccg gacgatgctc ctgatcaccc
      301 acgaccccgc tgaggccgag tggttcggga tccgcccgat cgagctcgcg cgacgcggga
      361 cctccgaacc ttctcgctag ccggccatcg ccggtcaccg cggacactcg ccgcggcatt
      421 tgttcgatgg gtgtagtaat accggagggc cgacggacgc catcggggac cgcacggcac
      481 cctgttcgac aacgaaaggt caacccatgc gctaccgcac actcgggaac agcggagcga
      541 tcgtctcgac gttcgctctg ggaaccatga ccttcggcac ggaggctgac gaggcgacct
      601 cgcacgccat cctcgacgac tacgtcgctg cgggcggcaa cttcatcgac acggccgacg
      661 tgtacagcgc gggggtctcg gaggagatca tcgggcgctg gctcgcggcg caccccgccg
      721 agcgcgagca ggtcgtgatc gccacgaagg gccgcttccc gatgggcgtc ggtcccaacg
      781 acctcggcac ctcccggcgc cacctcaccc gcgcgctcga cgcgtccctg cggcggctgg
      841 gcacagacca gatcgacctc taccagatgc acgcctggga cgccgtgacg ccgctcgagg
      901 agaccctgcg gttcctcgac gacgccgtcc gcgccggcaa gatcgcctac tacgggttct
      961 cgaacttcct cggctggcag ctgaccaagg ccgtgcacgt ggcacgcgcc agcggcttca
     1021 cgccgcccgt cacgctccag ccccagtaca gcctgctcgt gcgggagatc gagtccgaga
     1081 tcgtgcccgc agccctcgac gcgaacatcg gcctgctgcc gtggtcgccg ctcgcgggtg
     1141 gctggctcac tggcaagtac cggcgcgacg ccgacccgac gggcgccaca cggctcggcg
     1201 agaacccgga gcgcgggatg gaggcgtggg agccccgcaa caagcaagaa cgcacctggc
     1261 ggatcctcga gaccgtcgcg gagatcgcca cggcccaggg cgtcaaccag gcacaggtgt
     1321 cccttgcgtg gctggaggcg cagcccgccg tcacctcggt gatcctgggt gcccgcacga
     1381 ccgcccagct ggccgacaac ctgggcgccg cctccgtcgt gctcaccgcg ggcgagctcg
     1441 ggcggctcga cgaggtgagc gcacccgtcg tctccgacta cccctacggc acagccggcg
     1501 ccgcccagcg ccaccgcgcc aacgacggca tccgcgtcct gcgctgacga gcgctaccca
     1561 cgaagggccc gtgacctgca gcgatgtgag tcacgggccc ttcgccatcc tgtgggccga
     1621 gccgcggact cacacgctgc gcacaccgga gaccacggtc agcggtcagg ttcggtgcac
     1681 atcctgatcg ggcaaagccc tcaggcccaa ccccaggaga tctcatgaga agcaccccgt
     1741 cccccatccg caagcgcatc gccggagtcg cgctcgtcgg cgccatcgcc ggcgcaggag
     1801 tcgtggcagc cgctgcggcc gccaacgcgg ccgacagcac gcccacggcc acgacctcga
     1861 gcaccgccgc cacggatacg cccgccgcgg gcggcgccgt cgacgagagc aagagcgtcc
     1921 ggtccgacga gcacctgctc accggcaccg acgcggccag cgccaccgcc gcggcgctgg
     1981 ccaagtaccc cggcgccacc atccagcgcg tcgagaccga cagcgccggc gtctacgagg
     2041 cccacctgat caccgcggcc ggagaacggc tgatcgtcca gatggatgcc acgttcaccg
     2101 tcaccggcaa cgacaccggc ggcccggccg gcggcacccg cggcgccgac cccacggatg
     2161 cgccgtccac aaccaccg
//
LOCUS       DASRKH010000102         2342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_18785, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000102 DASRKH010000000
VERSION     DASRKH010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18785"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="VFC06_01760"
     CDS             complement(<1..571)
                     /locus_tag="VFC06_01760"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HZL80658.1"
                     /translation="MSAQLDDEVALRSIVVAQGVALEGAHPFEVLAWAGETFGPGLVV
                     ASSMGDEVLVHLAGRAVPGVDVIFLDTDYHFAETIGTRDAYAETTDITLRTILPLRTV
                     AEQDAEHGPRLHDRDPNLCCALRKVEPLERGLAPYRAWVTGMRREDAPTRTDITVVGW
                     DAKRAKVKLNPLAAWTQDDIDAYVAEHGVL"
     gene            complement(568..738)
                     /locus_tag="VFC06_01765"
     CDS             complement(568..738)
                     /locus_tag="VFC06_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80659.1"
                     /translation="MSAADVNSSGERVTAYYCPYCAGEDLRPAGERHGEWECRGCTRV
                     FAVRFIGLAVRS"
     gene            complement(735..>2342)
                     /locus_tag="VFC06_01770"
     CDS             complement(735..>2342)
                     /locus_tag="VFC06_01770"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HZL80660.1"
                     /translation="AFGDREPLNANEELKAADDGLNVRARIETVYARAGFASIPAEDL
                     RGRMRWWGLYTQRRPGIDGGRTATLEPHELDDEYFMLRVRCDGGRLTLRQLRVIGEIS
                     TELARGTADITDRQNIQLHWVRIEDVPEIWRRLEAVGLTTQEACGDVPRVILGSPVAG
                     VAADEIIDGTPAIEAIHRRYIGDPAFSNLPRKFKTAISGSPHHDVVHEINDVSFVGVV
                     HPELGPGFDLWVGGALAANPMLAVRLGAWVPLEAVPEVWEGVIGIFRDYGYRRLRNRA
                     RLKFLVADWGAAVFRDVLEQEYLGRRLDDGPEPPAPPHGRRDHVGVHPQRDGRFYVGA
                     APLVGRVSGPMLTSLVDLAERAGSDRVRLTVEQKVVVLDVAGDRVDDLVAALEQLGLQ
                     VTGASTFRRGTMACTGLEFCKLAIVETKARGAALVDELERRLPTFEHPLTINLNGCPN
                     SCARIQVADIGLKGVLAGGDEGFQIHLGGALGIDGHLGRTVRGLRITAEDLPDYVERV
                     ATRFEAQRADDEEFAQWALRADEAELR"
BASE COUNT          318 a          846 c          857 g          321 t
ORIGIN      
        1 gcaggacacc gtgctcggcg acgtaggcgt cgatgtcgtc ctgcgtccag gccgcgagcg
       61 ggttcagctt gaccttggcg cgcttggcgt cccagccgac gaccgtgatg tcggtgcgcg
      121 tcggtgcgtc ctcgcggcgc atgcccgtga cccaggcgcg gtacggcgcg agcccgcgct
      181 cgagcggctc gaccttgcgc agcgcgcagc aaaggttcgg gtcccggtcg tggagccggg
      241 gcccgtgctc ggcgtcctgc tccgcgaccg tgcgcagcgg caggatcgtg cgcagcgtga
      301 tgtcggtcgt ctcggcgtac gcgtcgcggg tcccgatcgt ctccgcgaag tggtagtcgg
      361 tgtcgaggaa gatcacgtcg acgcccggga ccgcccgacc ggccaggtgc acgaggacct
      421 cgtcgcccat cgaggacgcg acgacgagcc ctggcccgaa cgtctcaccg gcccaggcca
      481 gcacctcgaa cgggtgcgcg ccctcgagcg cgacgccctg cgcaacgacg atcgagcgca
      541 gcgcgacctc gtcgtccagc tgcgcgctca tgagcgcacc gccagaccga tgaaccggac
      601 cgcgaagacc cgcgtgcacc cgcggcactc ccactcgccg tggcgctcac ccgcggggcg
      661 caggtcctca ccggcgcagt acgggcagta gtacgccgtg acgcgctcac cggagctgtt
      721 gacgtccgcg gcgctcaccg gagctccgcc tcgtcggcgc gcagcgccca ctgggcgaac
      781 tcctcgtcgt cggcgcgctg ggcctcgaac cgcgtcgcga cgcgctcgac gtagtcgggc
      841 aggtcctccg cggtgatccg cagcccgcgc accgtccggc cgaggtggcc gtcgatgccg
      901 agcgcgccgc cgaggtggat ctggaagccc tcgtcgccgc ccgcgagcac gcccttgagg
      961 ccgatgtcgg cgacctggat ccgcgcgcac gagttggggc agccgttgag gttgatggtg
     1021 agcgggtgct cgaacgtggg gagccgtcgc tcgagctcgt cgacgagggc ggcgccgcgt
     1081 gccttggtct cgacgatcgc gagcttgcag aactccaggc ccgtgcaggc catcgtgccg
     1141 cgccggaacg tcgaggcgcc ggtgacctgc aggccgagct gctcgagcgc cgcgaccagg
     1201 tcgtcgaccc ggtcgcccgc gacgtcgagc acgacgacct tctgctcgac cgtgagccgg
     1261 acacggtcgc tgcccgcacg ctcggcgagg tcgacgagcg acgtcagcat cggcccgctg
     1321 acccgcccga ccaacggcgc cgcaccgacg tagaagcggc cgtcgcgctg ggggtgcacg
     1381 cccacgtggt cacgacgccc gtgaggtggc gcggggggtt cggggccgtc gtcgaggcgc
     1441 cggcccaggt actcctgctc gagcacgtcg cggaacaccg cggcacccca gtcggcgacc
     1501 aggaacttca ggcgcgcacg gttgcgcaga cgccggtagc cgtagtcgcg gaagatgccg
     1561 atcacgccct cccacacctc gggcactgcc tcgaggggga cccaggcgcc gagacggacg
     1621 gcgagcatcg ggttggccgc cagcgccccg ccgacccaca ggtcgaaccc ggggccgagc
     1681 tcggggtgca cgacaccgac gaacgacacg tcgttgatct cgtgcacgac gtcgtggtgc
     1741 ggtgaaccgc tgatcgcggt cttgaacttg cgcggcaggt tcgagaaggc ggggtcgccg
     1801 atgtaccggc ggtggatcgc ctcgatcgcg ggggtgccgt cgatgatctc gtcggcggcg
     1861 actccggcga ccggcgagcc gaggatgacg cgcggcacgt ctccgcacgc ctcctgggtc
     1921 gtcagcccga cagcctcgag ccggcgccag atctccggca cgtcctcgat gcgcacccag
     1981 tgcagctgga tgttctgccg gtcggtgatg tcggccgtgc cgcgggccag ctcggtcgag
     2041 atctcgccga tcacgcggag ctggcgcagg gtgagccggc ccccgtcgca gcggacccgc
     2101 agcatgaagt actcgtcgtc gagctcgtgc ggctcgagcg tcgcggtccg gccgccgtcg
     2161 atcccgggcc ggcgctgcgt gtacaggccc caccagcgca tgcgaccccg caggtcctcg
     2221 gccgggatcg acgcgaagcc ggcccgggcg tagacggtct cgatgcgggc gcggacgttg
     2281 aggccgtcgt cggccgcctt gagctcctcg ttggcgttga gcggctcgcg gtcgccgaag
     2341 gc
//
LOCUS       DASRKH010000103         2340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_13999, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000103 DASRKH010000000
VERSION     DASRKH010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13999"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VFC06_01775"
     CDS             complement(<1..267)
                     /locus_tag="VFC06_01775"
                     /inference="COORDINATES: protein motif:HMM:NF012576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZL80661.1"
                     /translation="MSTDVLDKSASCRADRAVEPLARRIDLSRTQPSLSRPILGAKVG
                     AEAPRRAGRRPSGRTHAQADRRTVATIGDVARAAGVSTSTVSYVL"
     gene            175..456
                     /locus_tag="VFC06_01780"
     CDS             175..456
                     /locus_tag="VFC06_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Beta-galactosidase C-terminal domain"
                     /protein_id="HZL80662.1"
                     /translation="MCARQIDSARKRFDGSIRTARRTFVKNVRGHGAVGPSRISKRFD
                     DGLFLLNSAADPRTVVLDGMHRDLLTGTDTTGAVDLGPEGAVALIERTS"
     gene            453..>2340
                     /gene="yicI"
                     /locus_tag="VFC06_01785"
     CDS             453..>2340
                     /gene="yicI"
                     /locus_tag="VFC06_01785"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HZL80663.1"
                     /translation="MKFNDGYWQVLPGVTILRPQAVDDVVVEPETLTVYAATGPLEKR
                     GDTLNRPLVTVSFHSPMDDVVGVTIEHFQGGVNRGPRFELATGESDVKVAGPERSGAP
                     VATFRSGALTARVATEGEWNVDFVAGDRPLTTSTPRSVGVITDAEGRHFVREQLTLGV
                     GEHVYGLGERFGAFVKNGQTVDIWNADGGTASDQAYKNVPFYLTDAGYGVFVDQPEKV
                     SFEVGTEVVSRAQFSVEGQSLRYYVIYGPTPKDILRKLTALTGRPARVPEWSYGLWLS
                     TSFTTDYDETTVTRFIDGMAERDLPLSVFHFDCFWMRRFHWCDFVWDPATFPDPEGML
                     ARLKAKGLKICVWINPYIAQRSALFAEGRELGYLVKNPDGSVWQWNLWQAGMGLVDFT
                     NPEATAWYQGKLKGLLDMGVDAFKTDFGERIPTDVVWHDGSDPAGMHNYYTHLYNKAV
                     FELLEEEKGEGEAVLFARSATAGGQQFPVHWGGDCESTFVSMAESLRGGLSLAMSGFG
                     YWSHDIGGFEGTPDPAVFKRWLAFGLLSSHSRLHGSDSYRVPWAFDDEAVDVTRRFTK
                     LKLSLMPYLARVGGEAHTAGTPVMRPMILEFPDDRGTAAIDTQYMLGDALLVAPVFNA
                     VGDVS"
BASE COUNT          356 a          772 c          822 g          390 t
ORIGIN      
        1 gagcacgtac gagactgtcg aggtcgagac tcccgcggct ctcgccacgt cgccgatcgt
       61 tgccaccgtg cgcctgtctg cttgggcgtg cgtccggccg gatggccgtc gtccggcgcg
      121 tctgggcgcc tccgcgccta ctttagcgcc gagtatgggg cgcgagaggg acggttgtgt
      181 gcgcgacaga tcgattcggc gcgcaagcgg ttcgacggct cgatccgcac ggcacgacgc
      241 acttttgtca agaacgtccg tggacacggc gcggtcggac cctctagaat atcgaaacgc
      301 ttcgacgatg ggctgttcct gctcaactcc gctgccgatc cgcgcaccgt cgtgctggac
      361 ggcatgcacc gagatctgct cacaggtact gacaccacgg gcgcggtcga cctcggtccc
      421 gagggcgccg tcgcgctgat cgagaggacg tcatgaagtt caacgatggc tactggcagg
      481 tgctgcccgg tgtcacgatc ctgcgaccgc aggcggtcga cgacgtggtc gtggagccgg
      541 agaccctcac ggtgtacgcc gcgaccgggc cgctcgagaa gcggggcgac accctgaacc
      601 gtccgctcgt gacggtgtcc ttccactcgc ccatggacga cgtcgtcggc gtgacgatcg
      661 agcacttcca gggtggcgtg aaccgggggc ccaggttcga gctcgcgacc ggggagtcgg
      721 acgtcaaggt ggccgggccc gagcgctcgg gtgcgccggt cgcgacgttc cgttcggggg
      781 cgttgacggc gcgcgtcgcg accgagggcg agtggaacgt ggacttcgtg gcgggcgacc
      841 gcccgctcac gacgtcgacc ccccgcagcg tcggcgtgat caccgatgcc gagggacggc
      901 acttcgtgcg cgagcagctg acgctcggtg tgggcgagca cgtctacggg ctcggcgagc
      961 ggttcggcgc gttcgtcaag aacggccaga cggtcgacat ctggaacgcg gacggcggca
     1021 cggcgtcgga ccaggcctac aagaacgtcc ccttctacct gaccgacgca gggtacggag
     1081 tcttcgtcga ccagcccgag aaggtctcgt tcgaggtggg caccgaggtg gtgtcgcgcg
     1141 cccagttcag cgtcgagggt cagtcgctgc gctactacgt catctacgga ccgactccca
     1201 aggacatcct gcgcaagctc acggcgctga ccgggcgtcc cgcgcgggtg cccgagtggt
     1261 cgtacggcct gtggctgtcg acgtcgttca cgacggacta cgacgagacc acggtcacgc
     1321 gcttcatcga cggcatggcc gagcgtgacc tgcccctgtc ggtgttccac ttcgactgct
     1381 tctggatgcg ccgcttccac tggtgcgact tcgtgtggga ccccgcgacg ttcccggacc
     1441 ctgagggcat gctggcccgg ctcaaggcca agggtctgaa gatctgcgtg tggatcaacc
     1501 cgtacatcgc acagcgctcg gcgctgttcg ccgagggacg cgagctcggc tatctcgtga
     1561 agaacccgga cgggtccgtc tggcagtgga acctgtggca ggccggcatg gggctcgtcg
     1621 acttcaccaa cccggaggcg accgcctggt accagggcaa gctcaagggg ctgctcgaca
     1681 tgggggtcga cgcgttcaag accgacttcg gcgaacggat tcccacggac gtcgtctggc
     1741 acgacggctc ggacccggcg gggatgcaca actactacac gcacttgtac aacaaggccg
     1801 tcttcgagct gctcgaggag gagaagggcg agggcgaggc cgtgctgttc gcccgctcgg
     1861 ccacggccgg cggccagcag ttcccggtgc actggggcgg cgactgcgag tcgaccttcg
     1921 tgtcgatggc ggagtccctg cgcggcggcc tgtcactggc gatgtcgggc ttcggctact
     1981 ggagccacga catcggcggc ttcgagggca cccctgaccc cgcagtgttc aagcggtggc
     2041 tcgccttcgg cctgctttcg tcgcacagcc gcctgcacgg ttcggactcg taccgggtgc
     2101 cgtgggcgtt cgacgacgag gcggtcgacg tcacgcgccg cttcaccaag ctcaagctgt
     2161 cgctcatgcc gtacctcgcg cgggtcggcg gcgaggcgca caccgcggga acgccggtca
     2221 tgcggccgat gattctcgag ttccccgacg accgcgggac ggcggcgatc gacacgcagt
     2281 acatgctcgg cgacgcgctg ctcgtcgcgc ctgtgttcaa cgccgtcggc gacgtgtcct
//
LOCUS       DASRKH010000104         2186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_39951, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000104 DASRKH010000000
VERSION     DASRKH010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39951"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VFC06_01790"
     CDS             <1..285
                     /locus_tag="VFC06_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80664.1"
                     /translation="ASAYLTLVTSPTHRHLVYNVCSGVSVGGTEILAHICEAMGIPVP
                     ATHADPTRYRPVDAQKVTGSSERLRKEFGWEPRYSLAQSIGDAVAYVADA"
     gene            complement(655..1506)
                     /locus_tag="VFC06_01795"
     CDS             complement(655..1506)
                     /locus_tag="VFC06_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HZL80665.1"
                     /translation="MSMADEAYRACQNAARAQAQATGKPVATDAVLTRHALESFLHRL
                     TLTEHADSFVLKGGVLLAAYDYRRTTRDVDSNAVAVDLTAGHVEQITRDLAQVEANDG
                     VDFETDSIRVETIREGAGYPGLRMRIQAMIATWRGVVAWDISTGDPIVPAPRTITIDR
                     VIGEPIRMLGYSVEMIIAEKGVTILERGSTSTRWRDYVDIVQLCSRGFDSNELRRSAA
                     AVAQYRQVTLGPVGPHLADYDDSGQRRWAAWRRKEDHEDDSDESLEAQLAFVARYLDP
                     VFERDRT"
     gene            complement(1503..2045)
                     /locus_tag="VFC06_01800"
     CDS             complement(1503..2045)
                     /locus_tag="VFC06_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80666.1"
                     /translation="MTTSHTEQFSRATLHRRAKSGDYTRIARGIYRPADAPPASPEWI
                     EAATRSPHATICLASALAHHNLTDAIPDTLEIALPRGTRPPATDSAITWRLFDTGTFD
                     IGRTQIPIPGTDMSIGLYSPERTLVDAFRLRGLIGYETATEALKTWLSSGGKPARLAE
                     IAVRLPRARGPVMRALEYLS"
BASE COUNT          358 a          683 c          730 g          415 t
ORIGIN      
        1 gcgagcgcgt acttgactct cgtcacgtcg ccaacccatc gccacttggt ctacaacgtc
       61 tgttccggtg tgtcggtcgg gggtaccgag atccttgcgc atatttgtga ggccatgggc
      121 atccctgtgc ccgctactca cgccgatccg acgcgctatc gacccgttga cgcgcaaaaa
      181 gtcaccggct ccagtgagcg tctgcgcaag gaattcggat gggagccgcg gtactcgctc
      241 gcgcaatcaa tcggagacgc cgtcgcgtac gtcgccgacg cgtaagagca acgactgaac
      301 cagcatcgcc cctggactgc ggcaccgttt cgcttcccta gaccgcgcct acgcgcagcg
      361 tctcgatgaa ccccagtatc tgtccctgca aggccgggaa ggctccgtcg agcggaacct
      421 catccttggc cgcctccgcg agttgatcga gcgtgtccgc aatcgtggcc gcagcccggc
      481 tcagccccca cgaggtgaac tcggcgagca gatcgtccct ggtgatgtcc gtgtgccgat
      541 acccacagcg aggatcacca tgcgggcgag gtccaccagg tccgcttcgg aggcgtgcct
      601 ggtgagcgtc tcggcaaccc gcccgaggct cacagtcggc agaatgtccg caactcacgt
      661 ccggtcgcgt tcgaataccg gatcgaggta gcgggcgaca aacgccagtt gcgcctcgag
      721 ggattcgtct gagtcgtctt cgtgatcttc tttgcgtcgc caggctgccc acctgcgttg
      781 gcccgagtcg tcgtagtcgg ccaagtgagg cccgacagga cccagggtga cctgtcggta
      841 ttgcgccacg gccgcagcgg agcgcctcaa ctcgttgctg tcgaaccctc gcgaacacaa
      901 ctggacgata tccacgtagt cgcgccatcg ggtgctggtc gaaccgcgct cgaggatcgt
      961 gacgcccttc tcggcgatga tcatctcaac gctgtacccg agcatccgga tcggctcacc
     1021 gatcacccgg tcgatggtga tggtgcgcgg ggcgggcacg atggggtccc cggtggatat
     1081 gtcccaggcc acgacgccgc gccaggttgc gatcatcgcc tggatgcgca ttcgaagtcc
     1141 ggggtacccg gcaccttcgc ggatggtctc gacacggatg gagtcggtct cgaagtcgac
     1201 accgtcgttt gcctcgacct gagccagatc gcgggtgatc tgctcgacgt gtcccgcggt
     1261 caggtcgacc gccaccgcgt tggagtccac gtctcgggtg gtgcggcggt agtcgtaggc
     1321 agcgagcaat acgccgccct tgagcacgaa tgagtctgcg tgctcggtca gggtaagtcg
     1381 gtgcaggaag gactccagcg cgtggcgtgt caagacggcg tcggtcgcga ccggcttacc
     1441 ggtcgcttga gcctgtgctc gcgccgcatt ctggcatgcg cggtaggcct cgtcggccat
     1501 gctcatgaca ggtactccag cgctcgcatc actggtccgc gggcgcgtgg cagccgtacg
     1561 gcgatctcgg ccagccgtgc gggcttgccc ccggagctga gccatgtctt gagtgcttcg
     1621 gtcgccgtct cgtagccgat caggccccgc aggcggaatg catcaaccag tgttcgttcg
     1681 gggctgtaga gcccgatgct catgtcggtg ccggggatcg gaatctgagt gcggccgata
     1741 tcgaaggtgc cagtgtcgaa caggcgccat gtgatcgcgg agtcggtggc aggaggacgg
     1801 gtgccgcggg gaagggcgat ctctagtgtg tctgggatgg cgtcggtgag attgtgatgg
     1861 gccagcgcgg acgccaggca gatcgtcgcg tgcggactgc gagtagccgc ctcgatccac
     1921 tcgggactcg cgggtggcgc gtcggctggg cggtagatcc cgcgcgcgat gcgcgtgtag
     1981 tcgcccgact tggcgcggcg gtgcagtgtt gcgcgcgaga actgctcggt gtgcgacgtc
     2041 gtcatggtgc gccccttccg agcattgaaa caaaacgtat gtagataagc gtatctgtag
     2101 gcagtatgtc tcgcaagggg gcggcggtga cctacaccgt tccgccacgc aggttgttga
     2161 taaaccccga ctggcctatc cattta
//
LOCUS       DASRKH010000105         2686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_14683, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000105 DASRKH010000000
VERSION     DASRKH010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14683"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(283..1680)
                     /locus_tag="VFC06_01805"
     CDS             complement(283..1680)
                     /locus_tag="VFC06_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HZL80667.1"
                     /translation="MSTTTAPRPTAQPEATTPVEDRRAVDRLGGVAVVVPVLLTALVL
                     VAGALGRIPENMIGGLAVITGLGMLLGPLGNRLPGISRVGGGALVCLMVPSLLVYLGF
                     FSENTLAATSALMKQANFLYFVICTLVVGSIFGMRRSMMLSGLARIFAPLLVGTLAAV
                     GVGTGVAMLFGYSFHRALFYLVVPIIGGGIGEGILPLSAAYSGALGGSAETYVAELIP
                     AAVVGNIVAIILAGALRRLGERRPQLDGGGMLVRPRPASDRPAAATVQAQAAAGAASS
                     VATVRGEHASPTSSAPTAYGMGVLVICSMFVLGTLAEPLVHLPAPVLVIVFSVILKVA
                     GLVPESVEDSARAVYSIICGHFVYPLMIGLGMLYVPLDNVLKVLGVGYVVTCAAVVLT
                     MAGAGFLVGRATGMFPIDAALVTVCHSGLGGTGDVAILSASQRMVLMPFAQISTRIGG
                     VTTVVAAASLIRLIS"
     gene            1938..>2686
                     /locus_tag="VFC06_01810"
     CDS             1938..>2686
                     /locus_tag="VFC06_01810"
                     /inference="COORDINATES: protein motif:HMM:NF012608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HZL80668.1"
                     /translation="MTVLATPSAAYTLTLRVELSSSPGTLGRLTTVIGAAGANIGSVD
                     VVSRGRHTLIRDIQVFCRDEAHGTQVMAAARTVTGTTVASVIDRTFALHRGGKIEITP
                     RFALDAREDLSIAYTPGVGRISSAIAQEPELVWDLTIKRNSVAVLTDGSAVLGLGDLG
                     PCAALPVMEGKAMLFKQFAGIDAYPICANAHTIEELVALGVMIAPGFGGINLEDISAP
                     RCFEVERRLQDLVDIPVFHDDQHGTATVVLAA"
BASE COUNT          462 a          972 c          889 g          363 t
ORIGIN      
        1 gatacggagg ccgcccaacc cggatggctg aaccttgcca tcgccggact tgccatcctg
       61 ctcctgggca ccgccgcact gctctacgtg cttcaccgac gcgcacccct gcgcagagac
      121 cggtgaccgg ctgaccttca acctcgcgtc ttcatggtgg ggtggcgcgg gtgggtgccg
      181 ccgtcgccgg cacggccagc tcgccgccgc gtcccgagcc ggagatcgcc cacggcggct
      241 cacgatgcca cccgggccac caggacgccc tttctggctc gctcagctga tgagccggat
      301 gagcgaggcc gccgccacga cggtggtgac ccctccgatg cgggtcgaga tctgcgcgaa
      361 cggcatgagc accatgcgct gcgacgccga caggatcgcg acgtcgccgg tcccgccgag
      421 cccgctgtgg cacacggtca cgagtgcggc gtcgatcggg aacatgccgg tcgcgcgccc
      481 cacgaggaat ccggcgcccg ccatggtcag gaccaccgcc gcgcacgtca ccacataccc
      541 cacgccgagg accttcagca cgttgtccag cggcacgtac agcatcccga ggccgatcat
      601 gagcgggtag acgaagtgcc cgcagatgat cgagtacacg gcccgcgccg agtcctcgac
      661 gctctccggc acgaggcctg caaccttcag gatcaccgag aagacgatga ccaggaccgg
      721 ggcgggcagg tgcacgagcg gctccgcgag ggtgccgagg acgaacatcg agcagatgac
      781 gagcacaccc atcccgtagg ccgtcggggc cgacgacgtc gggctggcgt gctcgccgcg
      841 cacagtggcc accgagcttg cggcgccggc ggctgcctgc gcctgaacgg tcgccgcagc
      901 cggccggtcg gatgcgggcc ggggccgcac gagcatcccg ccaccgtcga gctgtggtcg
      961 ccgctcaccg agccgacgca gcgcgccggc caggatgatc gccacgatgt tgccgacgac
     1021 cgcggccggg atgagctcag cgacgtacgt ctcggccgat ccgccgaggg cacccgagta
     1081 cgcggccgac agaggcagga tcccctcgcc gatgccacct ccgatgatcg gcacgacgag
     1141 atagaacagt gcccggtgga agctgtagcc aaacagcatc gcgaccccgg tgccgactcc
     1201 gacagcggcg agcgtgccca ccagcagcgg cgcgaagatc ctcgccagcc ccgacagcat
     1261 catcgagcgg cgcatgccga agatgctgcc gacgacgagc gtgcagatca cgaagtagag
     1321 gaagttcgcc tgcttcatca acgcactggt cgccgcgagg gtgttctcgc tgaagaaccc
     1381 gagatacacg agcagcgacg gcaccatcag acagaccaga gccccgccac cgacccggga
     1441 gatgccgggc agccggttgc cgagaggacc gagcagcatc cccaggcccg tgatcacggc
     1501 cagtccgccg atcatgttct ccgggatcct gccgagcgcg cccgcgacca gcaccagcgc
     1561 ggtcaacagc actgggacga cgaccgccac cccaccgagt cgatcgaccg cacgtcgatc
     1621 ctcaacgggt gtggtggcct cgggctgagc tgtggggcgt ggtgcagtcg tcgtcgacat
     1681 gggtcctccg gggatgagca cggcgtcgtt gccatcgacg ggcgagcccg tcgatgtgtg
     1741 tctagtgccc ggggtgtcgc acgtcacgct tgcgtacaca acggtcgtcg cgggcctacc
     1801 tccacgctcc cctgcgcacg acgtcgcgac cgttgtgtac gcaagcgtga ccgcgcatct
     1861 ggcgcgagct agaacggaac ggatggcggc acgccgtcct cggcaacgac gccgagccca
     1921 tcgccccgag gatcaccatg accgttctgg ccaccccgtc cgccgcctac accctcactc
     1981 tgcgagtcga gctcagcagc agccccggca cgctcggccg actgacgacc gtgatcggtg
     2041 ccgccggtgc gaacatcggg agcgtcgacg tcgtctcccg cgggcggcac acgctcatcc
     2101 gggacatcca ggtcttctgc cgcgacgagg cccacggcac gcaggtcatg gcggcggccc
     2161 gcacggtcac cggcacgacg gtcgcgtcgg tcatcgaccg cacgttcgcg ctccaccggg
     2221 gcggaaagat cgagatcacc ccgcgcttcg cactcgacgc acgcgaggac ctctcgatcg
     2281 cctacacccc tggggtcggc cggatctcgt cagcgatcgc acaggagccg gagctcgtat
     2341 gggacctgac gatcaagcgc aactcggtcg cggtgctgac ggacggctcg gccgtgctcg
     2401 ggctgggcga cctcggaccg tgtgctgccc ttccggtcat ggaaggcaag gcgatgttgt
     2461 tcaagcagtt cgcggggatc gacgcgtacc cgatctgcgc gaacgcccac acgatcgagg
     2521 agctggtcgc gctgggggtg atgatcgcac ccggcttcgg cggcatcaac ctcgaggaca
     2581 tcagcgcacc gcgctgcttc gaggtcgagc gccggttgca ggacctcgtc gacatccccg
     2641 tcttccacga cgaccagcac ggcacggcca ctgtcgtact cgcggc
//
LOCUS       DASRKH010000106         2945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_59310, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000106 DASRKH010000000
VERSION     DASRKH010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59310"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1509
                     /locus_tag="VFC06_01815"
     CDS             <1..1509
                     /locus_tag="VFC06_01815"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HZL80669.1"
                     /translation="YLERVSPEGQYQRGTERVDLETRTEEVLVADGEPFTVEIQSTIH
                     GPIISEAMPSTEVASGSPVPAGSPTGGLDGYAVSLAWTALTPGRTLEALIDLDRAATP
                     ADVAAAAAKFEVPAQNIVYATTAGDIGHQAAGRIPIRSLAVSGAVPVDGTWPRPGWDP
                     AYDWEGFLSAENLPSTLNPAEGFIVAANQAVTVSGGGAFLTADFDYGFRAQRLRSLLI
                     AQIEAGEKVTVEGANALMMDSAHPLAGVMVPAILRLNIEQEFVREGVRELRTWREQGY
                     PTDVDSAGAAYFNTVWANVLALTFADELPADVVTSGDSRWIQVVTLLMDDPRNAWWDD
                     VSTVRVTETRDEILVRAVENARYQLTNTLGKDAERWRWGDLHQARFEHPVLTPATTHG
                     VLAWLTNPTPFEVSGTSASVNSTSWVASMEGGDRADFTMTAAPSMRMVVDMSDLDAST
                     WVVTTGTSGHPTSSHYADQVGAWAAGRYYPWVSSREAVEEAAASTLTLRPAK"
     gene            complement(1547..2212)
                     /locus_tag="VFC06_01820"
     CDS             complement(1547..2212)
                     /locus_tag="VFC06_01820"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HZL80670.1"
                     /translation="MREPLRLPQTVGYETEDAKHALRQVIRRHRTLRSRGELETLGEA
                     LATSALQAIGDARNVALYVSVGGEPPTLPLLERLRARDVAVLLPALGPGLARSWSFYR
                     GLDDLQTRAPGRPPEPSGAVLGAEAIRSVDVVITSALAVDGYGGRLGQGGGWYDRMMK
                     MLDPETPTFAMLFDDELVSTQSLPQDENDVRIKAVITPTRVFLIAGSRLERDTLAAVA
                     GPN"
     gene            2282..>2945
                     /locus_tag="VFC06_01825"
     CDS             2282..>2945
                     /locus_tag="VFC06_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdenumtransferase MoeA"
                     /protein_id="HZL80671.1"
                     /translation="MRTVNDHLKACLSAVGQLPPLAVQLPDAVGCVLAEDVRAPSDLP
                     VADLAALDGYALRAADVAGADRGPVRLRVMDEVRAGASDAVTLVPGSVVRIASGAPLP
                     RGADAVVPLEGTDQGDAVVAVLRAVAAGVNVRPRAIDTRSGDIVLAAGERVGARQVAL
                     LAAVGRGRVLVHPKPRVVIVSVGDELVEPGTPAQSGQVFDANGHALATGVQDAGAVAL
                     RVG"
BASE COUNT          392 a         1074 c         1069 g          410 t
ORIGIN      
        1 tacctggagc gtgtctcccc ggagggccag taccagcgtg gcaccgagcg cgtggacctc
       61 gagacgcgca ccgaggaggt gctggtggcc gacggggagc ccttcaccgt ggagatccag
      121 tccaccatcc atggaccgat catctccgag gcgatgccca gcaccgaggt ggcctccggg
      181 tctcccgtgc cggcgggcag ccccacgggt ggcctggacg gctacgcggt gtcgctcgcc
      241 tggacggccc tgacgcccgg gcgcaccctg gaggccctca tcgacctgga ccgggccgcc
      301 acccccgcgg acgtcgccgc cgcggccgcg aagttcgagg tcccggcgca gaacatcgtc
      361 tacgccacca cggccgggga catcggccac caggccgccg gccgcatccc catccgctcg
      421 ctcgcggtct ccggcgcagt ccccgtggac gggacctggc cgcgtccggg gtgggacccg
      481 gcctatgact gggaggggtt cctctccgcc gagaacctcc cgagcacgct gaaccctgcc
      541 gagggcttca tcgtggccgc aaaccaggcc gtcaccgtga gcggcggggg cgcgttcctc
      601 acggcggact tcgactacgg cttccgcgcg cagcggctgc ggtcgttgct gatcgcgcag
      661 atcgaggccg gtgagaaggt caccgtggag ggcgccaacg cgttgatgat ggattccgcc
      721 cacccgctgg ccggcgtcat ggtgcccgcg atcctgcggc tcaacatcga gcaggagttc
      781 gtgcgggagg gcgtgcgcga gctgcgcacc tggcgggagc aggggtaccc gacggacgtg
      841 gactcggcgg gagccgccta cttcaacacg gtgtgggcca acgtgctcgc cctgaccttc
      901 gcggacgaac tgccggcgga tgtggtcacc tccggggatt cacgctggat ccaggtggtc
      961 acgctcctca tggacgaccc ccgcaacgcc tggtgggacg acgtctccac cgtgcgggtc
     1021 accgagaccc gcgacgagat cctggtccgc gccgtcgaga acgcccgcta ccagctcacg
     1081 aacaccctcg gcaaggacgc ggagcggtgg cggtggggcg acctccacca ggcccggttc
     1141 gagcaccccg tcctcacgcc ggcgacgacg cacggcgtgc tcgcgtggct caccaacccg
     1201 accccgttcg aggtctcggg gaccagcgcg tcggtcaact ccaccagctg ggtggcctcc
     1261 atggagggcg gcgaccgcgc cgacttcacc atgacggccg ccccgtccat gcggatggtg
     1321 gtggacatgt ccgacctcga cgcctccacc tgggtggtga cgacggggac gtcgggccac
     1381 ccgacgtcgt cgcactacgc cgaccaggtg ggtgcctggg ccgcgggccg gtactacccc
     1441 tgggtgtcca gccgggaggc cgtggaggag gccgcggcga gcacgctcac gttgcggccg
     1501 gcgaagtagc accgcgcccg gagccatcag accgcgcccg gagtcgtcag ttcgggccgg
     1561 cgaccgccgc gagggtgtca cgctcgaggc gcgaacccgc gatgaggaaa acccgcgtgg
     1621 gcgtgatgac ggccttgatc cgcacgtcgt tctcgtcctg cggtaggctc tgcgtggaca
     1681 cgagctcgtc gtcaaagagc atggcgaacg tcggcgtctc ggggtcgagc atcttcatca
     1741 tgcggtcgta ccagccgccg ccctgaccca gccggccgcc gtacccgtcc accgcgagtg
     1801 ccgaggtgat gaccacgtcc accgaccgga tggcctccgc ccccagtacc gctccggacg
     1861 gctccggcgg gcgcccgggc gcgcgggtct ggaggtcgtc cagcccgcgg tagaaggacc
     1921 agctgcgcgc cagtccgggc ccgagggcgg gcagcagcac cgccacgtcc cgggcgcgca
     1981 ggcgctccag cagtggcaac gtcggtggct ccccgcccac ggatacatag agcgcaacat
     2041 tgcgcgcgtc gccgatggcc tgcagggcgc tggtggccag cgcctctccg agcgtctcga
     2101 gctccccgcg gctccgcagg gtgcggtgcc gtcgaatcac ctggcgtaac gcgtgcttgg
     2161 cgtcttctgt ttcgtatcca actgtctggg gtagccgcaa aggctctcgc atgggaccca
     2221 ttgtgtcagg tggggccgac accatcccat cctgtccggc gagtcccgat agccttcacc
     2281 tatgcgcacc gtcaacgacc acctcaaggc gtgcctctcc gccgtgggcc agctgccgcc
     2341 gctcgccgtc cagctgcccg acgccgtggg ctgcgtgctc gccgaggatg tccgggcgcc
     2401 ttcggacctc cccgtggctg acctcgccgc gctggacggg tacgcgctgc gggccgccga
     2461 cgtcgccggg gccgatcggg gaccggtgcg gctccgcgtc atggacgagg tgcgggccgg
     2521 ggcgagtgac gccgtcaccc tggtccccgg ctccgtggtg cgcatcgcgt ccggcgcgcc
     2581 gctgccgcgg ggggccgacg ccgtcgtgcc gctcgagggg accgaccagg gcgacgccgt
     2641 cgtggcggtc ctgcgggccg tggcggccgg ggtgaacgtg cggccgcgcg cgatcgacac
     2701 ccgcagcggt gacatcgtgc tcgccgcggg cgagcgggtg ggcgcccggc aggtcgcgct
     2761 gctcgcggcg gtcgggcggg gacgcgtcct cgtgcacccc aagccccgcg tggtcatcgt
     2821 gtccgtgggg gacgagctgg tggagcccgg gacgccggcg cagtccgggc aggtcttcga
     2881 cgccaacggc cacgccctcg ccacgggcgt gcaggacgcc ggcgccgtcg cgctgcgggt
     2941 gggtg
//
LOCUS       DASRKH010000107         2083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_50062, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000107 DASRKH010000000
VERSION     DASRKH010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50062"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1395)
                     /locus_tag="VFC06_01830"
     CDS             complement(<1..1395)
                     /locus_tag="VFC06_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80672.1"
                     /translation="MLAKFRSAFVRNAKRDDGEVLLSAIVVMVVVGMFIMFMTTQAWG
                     ISVNAKHTQREALAQEAVDIALSQAIYTLNENYGVVSDNGSYDTANGAYSRTLPAASS
                     PASADLDSFNATKWTSFNGSPAGATKYGIQAQARWWIDTFDVSYDKPNTVDQNRVRFG
                     GVIGNLTVEARVVTPDETVVYGYRAITVPLYQFQARAVQSSSDGVGLAYVLSPLSMFQ
                     HGVFGTQSVQDLGEDGFVKAWSPIASSGDITLDDPVDSTNLNDDQQRRYDADLMLFGQ
                     GRCLEYDTTSGSVVGECSSVKTLNTGFEVVPDTNLVSQMQEFCSTQGSEDFIASQQAF
                     PSLTANDTTYCFRDFVMDANVNLPTNGASGRNVARIFVERNVIVKDGHHFLDENGTDS
                     FDAPATDPQVAIYTLSTAVTLEGNSSGSSHSNLYLYAPNATCSPGMRADDPVTTSVDE
                     SLGRSWDMYLGSIVC"
     gene            complement(1469..>2083)
                     /locus_tag="VFC06_01835"
     CDS             complement(1469..>2083)
                     /locus_tag="VFC06_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80673.1"
                     /translation="IMSTAFVALLATAWFVPGGLADRYMPIPGIVPGTFVSEDGTIVD
                     IPAGEPLSESVLAEVKAGAEGVAVETGPSGINKYLGDTFSLTVTHYIYITPQQISCDG
                     VEFFDGFLVAGDAVLDADDKEDPAQCEMLRTLDGARALVDQLIADYPFPNETVARLEI
                     NVDKPIEQADSALPFDESEIVYQSAHPEPHVMTADEKQTDAGSA"
BASE COUNT          383 a          640 c          660 g          400 t
ORIGIN      
        1 gcacacgatg gaccccaaat acatgtccca actgcggccg agagactcat cgacggaggt
       61 ggtgacgggg tcgtcggctc tcatacctgg gctgcacgtc gcgttgggcg catacaggta
      121 caagttggag tgggacgagc ccgacgagtt gccttccagc gtgaccgcgg tggacagcgt
      181 gtagatggcc acctgcgggt cagtcgccgg agcgtcaaac gagtccgtgc cgttctcgtc
      241 aaggaagtgg tgcccgtcct tcacgatgac gttgcgctcc acgaagatgc gcgccacgtt
      301 gcgtccgctc gcaccgttcg tcggaaggtt gacgttggcg tccatcacga agtcgcggaa
      361 gcagtacgtg gtgtcgttcg cggtcagtga cgggaacgcc tgctgtgagg cgatgaagtc
      421 ttcggaaccc tgcgttgagc agaactcctg catctgtgac acgaggttcg tgtccgggac
      481 gacctcgaag ccggtgttca acgtcttgac cgaggagcac tcgccgacga cggagcccga
      541 tgtggtgtca tattccaggc agcggccctg cccgaagagc atcaagtcgg cgtcgtaccg
      601 acgctgctga tcgtcgttca agtttgttga gtccaccggg tcgtcaaggg tgatgtctcc
      661 gctggaagcg atcggcgacc aggccttcac aaagccgtcc tcacccaggt cctgcacgct
      721 ctgcgtgccg aagaccccgt gctggaacat cgacaagggc gacagcacgt atgccaggcc
      781 cacgccgtcg ctcgaggatt gcaccgcacg agcctggaac tggtagagcg gcaccgtgat
      841 ggcgcggtag ccgtacacga ctgtttcgtc aggggtcacc acgcgggctt cgaccgtgag
      901 gttgccgatc acaccgccga agcggacgcg gttctgatcg accgtgttgg gcttgtcgta
      961 cgacacatcg aacgtgtcaa tccaccagcg tgcctgagcc tggatgccgt acttcgtggc
     1021 gcccgccggg ctgccgttga aggaagtcca cttggtcgcg ttgaaggagt ccaggtcggc
     1081 gctggccggc gacgacgcgg caggaagcgt acggctgtac gcaccgttcg ccgtgtcata
     1141 cgacccgttg tcagagacga ccccgtagtt ctcgttcagg gtgtagatcg cctgggaaag
     1201 ggcgatgtcg acagcctcct gggccagagc ctcacgctgg gtgtgcttgg cgttcacaga
     1261 gatgccccac gcttgcgtgg tcatgaacat gatgaacatg cccaccacaa ccatgacgac
     1321 aatggctgat agcaagactt cgccgtcgtc gcgcttcgca ttgcgtacga atgcgcttct
     1381 gaacttggcg agcatgccgt cccctcccgt gctgaaagtg ccgttgtggc accacaagcc
     1441 taccgcaccg gtacgacacg gattctggct aggcacttcc ggcgtctgtt tgcttctcgt
     1501 ccgcggtcat gacatgcggc tccgggtggg cgctctggta gacgatctcg gattcatcga
     1561 agggcaaagc ggagtccgcc tgctcgatcg gcttgtcgac gttgatctcg agtcgcgcaa
     1621 ccgtttcgtt cgggaacggg tagtcggcaa tcaactggtc gaccaatgcg cgagcgccat
     1681 caagggtccg cagcatctcg cactgcgccg gatcttcctt gtcgtcggcg tcgagcaccg
     1741 cgtctcctgc gacgaggaac ccgtcgaaga actcgacccc gtcgcaggat atctgctgag
     1801 gcgtgatgta gatgtagtgg gtaaccgtca gactgaaggt gtcgccgagg tacttgttga
     1861 tgcccgacgg tccggtttcc accgcgactc cctctgcccc cgctttgact tccgcgagca
     1921 cagactcgga caacggctcg cccgccggga tgtccacgat ggtgccgtcc tccgaaacga
     1981 acgtgccggg cacaatcccg gggatcggca tgtaccggtc cgccagccct ccagggacga
     2041 accacgccgt cgcgagaagc gccacaaacg cggtggacat gat
//
LOCUS       DASRKH010000108         2226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_63266, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000108 DASRKH010000000
VERSION     DASRKH010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63266"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /gene="purH"
                     /locus_tag="VFC06_01840"
     CDS             complement(<1..885)
                     /gene="purH"
                     /locus_tag="VFC06_01840"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136433.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HZL80674.1"
                     /translation="MSHELTPLAPVADATGPATRRPLRRALVSVYDKTGLVELATALH
                     AAGVELVSTGSTAATIAGAGVPVTKVEELTGFPECLDGRVKTLHPRVHAGILADTRRP
                     EHVAQLAELGIEPFDLVVVNLYPFSATVASGAGPDECVELIDIGGPSMVRAAAKNHPS
                     VAVVIDPARYADVATAAAEGGFTLAERRGLAADAFAHTAAYDTAVAAWFAGEYAPDAE
                     AARSGFPAFAGAAWTRARVLRYGENPHQGAALYLADDGAAGRGLAGATQLHGKEMSYN
                     NYVDADAAWRAAHDHDGPA"
     regulatory      complement(929..1022)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01750"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ZMP/ZTP riboswitches appear to regulate genes
                     involved in one-carbon metabolism; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="5-aminoimidazole-4-carboxamide
                     ribonucleotide (ZMP)/
                     5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl
                     5'-triphosphate (ZMT)"
                     /db_xref="RFAM:RF01750"
     gene            complement(1027..1827)
                     /locus_tag="VFC06_01845"
     CDS             complement(1027..1827)
                     /locus_tag="VFC06_01845"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZL80675.1"
                     /translation="MSRAPLSLANTARAEQTRPVENRPDAPRGARERARAEVMSELLA
                     SARARLGTDGAANLSLRAVARDLGVSSSAVYRYVESRDALLTLLIVEAYDAVGQVAEG
                     AAARARAAGSPPAQTWLAVARGVRGWALANRHSFELIYGTPVPGYQAPQDTVAAALRI
                     WAVIVSIMLEALDDGSLTPAGPDFDPDGLVTAEVFAFAGMGAPGDVPTESARGIVRSF
                     TLFASLIGALTAELFGHLHRVADDFAGVFDAMIATAAAGVGLHIDLLG"
     gene            1898..>2226
                     /locus_tag="VFC06_01850"
     CDS             1898..>2226
                     /locus_tag="VFC06_01850"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZL80676.1"
                     /translation="MSHHVILGKGPIGSTLARQLADAGHEVLVLSRSGAPSANGTPDA
                     SAESPIRHAAVDSADASALTRAARGADVLYNCVNPAYHRWPTDWPPIADAVLTAAEST
                     GAVLVTTG"
BASE COUNT          302 a          821 c          818 g          285 t
ORIGIN      
        1 ggccgggccg tcgtggtcgt gggccgcgcg ccacgccgca tcggcgtcga cgtagttgtt
       61 gtagctcatc tccttgccgt gcagctgggt cgcgccggcg aggccgcggc ccgcggcgcc
      121 gtcgtcggcc aggtagagcg cggcgccctg gtgcgggttc tcgccgtagc gcagcacgcg
      181 cgcacgcgtc cacgccgcac cggcgaatgc ggggaagccc gatcgggccg cctcggcgtc
      241 gggcgcgtac tcccccgcga accaggcggc gacggcggtg tcgtaggccg cggtgtgtgc
      301 gaacgcgtcg gccgcgaggc cgcgccgctc ggcgagcgtg aagccgccct cggccgcggc
      361 agtcgcgacg tcggcgtacc gcgcggggtc gatgaccacc gcgacgctcg ggtggttctt
      421 cgcggcggca cgcaccatgg acggcccgcc gatgtcgatg agctcgacgc actcgtccgg
      481 tccggcgccc gatgcgacgg tggccgagaa cgggtagagg ttgacgacga cgaggtcgaa
      541 cggctcgatg ccgagctcgg cgagctgcgc cacgtgctcg ggtcgccgcg tgtccgcgag
      601 gatcccggcg tgcacgcgcg ggtggagcgt cttgacccgg ccgtcgaggc actcggggaa
      661 cccggtcagc tcctcgacct tcgtcaccgg caccccggcg cccgcgatgg tggcggcggt
      721 cgagccggtc gagacgagct cgacgcccgc ggcgtgcagt gccgtcgcga gctcgacgag
      781 cccggtcttg tcatagacgc tgacgagcgc ccgccggagg gggcggcgcg tggcagggcc
      841 ggtcgcgtcg gcgacggggg cgagcggggt gagttcgtga gacatgggtg acctcccggg
      901 tcgttgcggt ctcgtgagac ttcgtggacc cgagcaccca ggcgaacggt gcgacggcgt
      961 gatgctgtgg ccgctccccg gtggttcgtt ccacctcagg tgcgccagtc acggccaggg
     1021 ctgagtctac ccgaggaggt cgatgtgcag gccgactccc gcggccgcgg tcgcgatcat
     1081 cgcgtcgaac accccggcga agtcgtccgc gacccggtgc aggtggccga acagctcggc
     1141 cgtcagcgca ccgatgagcg acgcgaagag cgtgaacgac cggacgatgc cgcgcgcgga
     1201 ctcggtcggg acgtcacccg gcgcgcccat gcccgcgaac gcgaacacct cggccgtcac
     1261 gagcccgtca gggtcgaagt ccggtcccgc cggcgtcagc gacccgtcgt cgagcgcctc
     1321 gagcatgatc gagacgatca ccgcccagat ccgcagcgcc gccgcgacgg tgtcctgcgg
     1381 cgcctggtac ccgggtaccg gggtgccgta gatcagctcg aacgagtgcc ggttcgcgag
     1441 cgcccagcca cgcacccccc gggcgacggc cagccaggtc tgcgccggcg gcgagcctgc
     1501 cgcgcgcgcg cgtgccgcgg ccccctcggc gacctgaccg acagcgtcgt aggcctcgac
     1561 gatcagcagc gtgagcagcg cgtcccgcga ctcgacgtag cggtagaccg ccgacgacga
     1621 gacgccgagg tcacgcgcca ccgcacgcag tgagaggttc gcggcgccgt cggtgccgag
     1681 ccgcgcgcgc gctgacgcga gcagctcgct catgacctcg gcgcgggcac gttcacgggc
     1741 gccgcgcggc gcatcggggc ggttctccac cggacgagtc tgctcggcgc gagcagtgtt
     1801 cgcaagcgag agcggtgctc ttgacatggc ccgtatcgac tgcgagcatt gctccatacc
     1861 gagagcaccg ctctcccctt cgcgacagga gcacgtcatg tcccaccacg tcatcctcgg
     1921 caagggcccc atcggctcga cgctcgcccg tcagctcgcg gacgccgggc acgaggtgct
     1981 cgtcctcagc cgttcgggcg caccgagcgc gaacgggacg cccgacgcga gcgccgagtc
     2041 cccgatccgc catgccgcgg tcgacagcgc cgacgcctcg gcgctcaccc gcgcggcccg
     2101 gggcgccgac gtcctctaca actgcgtcaa ccccgcctac caccgctggc cgaccgactg
     2161 gcccccgatc gcggacgccg tcctgacagc ggcagagtcc acgggggcag tcctcgtcac
     2221 cacggg
//
LOCUS       DASRKH010000109         2418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_14214, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000109 DASRKH010000000
VERSION     DASRKH010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14214"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VFC06_01855"
     CDS             <1..399
                     /locus_tag="VFC06_01855"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZL80677.1"
                     /translation="GLLDQVVAAGVPCALVTMSIGALVDAFLDEAGDVFAAVVMGDQV
                     RNGKPDPEAYLTAARRLEKDPRQCVAIEDSPAGVRSAAASGAVTLAVRRHVALPLLPG
                     VSRLASLDGVGLDGLAHFFAGQVQDDFAEG"
     gene            complement(396..1241)
                     /locus_tag="VFC06_01860"
     CDS             complement(396..1241)
                     /locus_tag="VFC06_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HZL80678.1"
                     /translation="MSRTPGLSPSRANDFAQCPHMYRLRVIDRVPEPPQRATTLGTLV
                     HLVLERLFDVPAAQRDPDAAHALLEPAWDDLVADRPELAELVADAAERSQFFGDARAR
                     VSTYFIVENPTRLEPHGREVRLESSLDGGPAIRGVIDRIDRAPDGALRIIDYKSGKAP
                     SSRYGEQADFQMKFYAMLVERTEGTRPALMRLLYLRDGGVKELVPTEADIAAVEAKVR
                     STWTAITMYAAAGAFPTSPSRLCAWCSFQTLCPEFGGTPPPLDPDAVERATGVRPESP
                     ETALP"
     gene            1468..2193
                     /locus_tag="VFC06_01865"
     CDS             1468..2193
                     /locus_tag="VFC06_01865"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HZL80679.1"
                     /translation="MKEVVLTLWGGHTSFESSDMTPTVNGVTAAAGPAMNLVIAGTAS
                     ATMAWTGLGGVGGTVVSYVAWANLLLAVFNLLPGIPMDGGRVLESIVWAITRSHHRGT
                     VVAAWGGRVVAVAVVVYSIAGPLLSGGRPSLTSVIVAVVVFSILWPAASSALAFSSTM
                     LRRDGVSAAGLMRGAVAVPYTYSVHEALGAAATAGVEEIVVLGADDAPAGRFPIEQAQ
                     AVPESARPTTLRATGSRTRRRRR"
     gene            complement(2294..2418)
                     /locus_tag="VFC06_01870"
                     /pseudo
     CDS             complement(2294..2418)
                     /locus_tag="VFC06_01870"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="site-2 protease family protein"
BASE COUNT          371 a          780 c          874 g          393 t
ORIGIN      
        1 gggttgcttg accaggtggt cgccgcgggc gtgccctgcg cgctggtgac catgtccatc
       61 ggggctctgg tggacgcatt tctggacgag gccggcgacg tgttcgcggc ggttgtcatg
      121 ggggatcagg tgcgcaacgg caagcccgat cccgaggcct acctgacggc ggcccggcgg
      181 ctcgagaaag atccgcgtca gtgtgtcgcg atcgaggact ctcccgccgg tgtccgcagc
      241 gccgcggcat cgggggcagt gacgctcgcg gtgcggcgtc acgtcgcgct acccctctta
      301 ccaggagtct cccgcttggc cagcctcgat ggtgtcggtc ttgacggact cgcccacttc
      361 tttgccggcc aggtgcagga cgatttcgca gagggttagg ggagggccgt ctcgggcgac
      421 tccgggcgca cccccgtggc gcgttcgacg gcatccgggt ccaatggggg aggagtcccg
      481 ccgaactcgg ggcagagtgt ctgaaaggag caccacgcgc acagtcgtga cggcgacgtg
      541 ggaaacgcgc ccgccgccgc gtacatagtg atcgcggtcc aggtggagcg aaccttcgcc
      601 tcgacggcgg caatgtcggc ctcggtgggc acaagttcct tgacgccgcc gtcgcggagg
      661 tacagaagcc gcatcagcgc gggtcgcgtg ccttcggtgc gttccaccaa catcgcatag
      721 aacttcatct ggaagtccgc ctgttcgccg taccgggagc ttggcgcctt gccggacttg
      781 tagtcgatga tccgcagcgc accgtcgggc gccctatcga tcctgtcgat gaccccgcga
      841 atggcgggcc ccccgtcgag agatgactcg agtcgcacct cacggccgtg gggttccagg
      901 cgcgtcgggt tctccacgat gaagtacgtg gacacgcggg ccctggcgtc gccgaagaat
      961 tgcgagcgct ccgccgcgtc cgcgacgagc tcggcaagct cggggcgatc agcgacgagg
     1021 tcatcccagg cgggttcgag cagcgcatgc gcggcatcag ggtcgcgctg ggcggccgga
     1081 acgtcaaaaa gccgttcgag aaccaagtgc accagggtgc ccagcgtggt ggcacgttgg
     1141 ggaggttccg gcacccggtc gatgacgcgg agccggtaca tgtgagggca ttgcgcgaag
     1201 tcgttggcgc gcgacgggga caggccaggt gttctcgaca tgggtccagc gtaggaccgg
     1261 aggccgacac ctcctagagc ggctagcgtg atcccatgga agagcgacgg acgcgagggc
     1321 tcgtagtggg ccgcgtgctt ggcgcgcacg tgatcatcca gcctggcacg ttggtgatga
     1381 ttgcgctgct cgcgtacttc ttcgcgtccg cgtcgggcga aacgacggtg cgcaccttgt
     1441 cgatcggcgc gaccaaacga gcgcgccgtg aaggaggtcg ttctcaccct gtggggcggc
     1501 cacacgagtt tcgagtcgag cgacatgacg ccgacagtca acggcgtgac cgcggcggcg
     1561 ggacccgcca tgaacctcgt catcgccggg accgcaagcg cgactatggc atggacgggc
     1621 ctcggcgggg taggcgggac ggtcgtgtcc tacgtcgcct gggcaaacct gctgctcgcc
     1681 gtgttcaacc tgttgcccgg catcccgatg gatggcgggc gggtcctcga gtcgatcgtc
     1741 tgggcgatca cgcgtagcca ccaccgtggc accgtcgtgg cggcgtgggg cgggcgggtc
     1801 gtcgccgtcg ccgtcgtcgt gtattcgatc gcgggccccc ttctgagcgg aggccgaccg
     1861 tcgctgacca gtgtcatcgt cgccgtcgtc gtgttctcga tcctgtggcc cgcagcgtcg
     1921 tcggcgttgg ccttttcctc gacgatgctc cgtcgagatg gggtcagcgc ggccgggctc
     1981 atgcgcgggg ctgttgcggt gccgtacacg tactcggtgc acgaggcgct cggcgccgcc
     2041 gccaccgcgg gagtggaaga gatcgtcgtc ttgggtgccg acgatgcgcc agcgggcagg
     2101 ttcccgatcg agcaggccca ggccgttccc gagtcggccc gcccgacgac gctgcgggcc
     2161 acaggatcga gaacacgacg acggcgacga tgacgcttgt cagcgacggt cggcctccac
     2221 tcagaagggg gcccgcgatc gaaaccacga cgacgacgac cgcgacgacc cgcccgcccc
     2281 acgccgcgac gacggtgcct cgatggcggc tgcgcgtgat cgcccagacg atcgactcga
     2341 ggacccgtcc accatccatc gggatgccgg gcaacaggtt gaacacggcg agcagcaggt
     2401 ttgcccaggc gacgtagg
//
LOCUS       DASRKH010000110         2859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_58901, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000110 DASRKH010000000
VERSION     DASRKH010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58901"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="VFC06_01875"
     CDS             <1..806
                     /locus_tag="VFC06_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80680.1"
                     /translation="VISKWHTVGPDFSGELNAVLSEMGSTPVEDRIPVLAFGSNACPA
                     QLRHKAEQSGFRLDIPLLEVVVAGVTVGHSATLSSRGYFPMTGVPRSSSIARGHLMLL
                     DEHQLMAMDRSEPGFQRVLLSSRDGYALECGGVRLATTYMYVAREGHLIDKDNGSTLY
                     DMDHEALLSRLVSSPGDLRALLGDDPEEIVARARRSTSGWLHTVVDTLHAAGWVERRD
                     PANLLECHKDRRPMYFGAQESPTRVLWHTESDMADREQNSVSVSPILLW"
     gene            1067..2080
                     /locus_tag="VFC06_01880"
     CDS             1067..2080
                     /locus_tag="VFC06_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80681.1"
                     /translation="MRTIDWAEFARTIDAEGDALAALALAGEWLDSAERGRALASDDG
                     SKVTYRWIKALDVAQWHHNEGETISGCGRRLGVTRQRAQQLLWLVESEQKFDVPDRAA
                     DDARARARVLARVFSESVAILRHAPGKTVHEMAEQISCDEVDVSLVLGPLAAFTINPS
                     LSTRLGRSKWSDDQIASTLRDCYAANDNTPLTKRAYAELTAKNSPPPPSQALIAKRFG
                     TWNEALVHAGLPSNDRPHEYTWAHTREEALGNMVEFVLATGKTSVGAYSAWSEKRDDV
                     IRFPHAVVGEGQSWTDAIRQVFAVVSRPPHVAAYRSVLLARAGEQAAALAGKFDADFA
                     ARL"
     gene            2382..>2859
                     /locus_tag="VFC06_01885"
     CDS             2382..>2859
                     /locus_tag="VFC06_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80682.1"
                     /translation="MHTVTADFQDEVHVEDPDLAALVEIRAAYGTPVAINAADTERWF
                     VISRGNLRDRDYIRFTTAMGRTVEGTARKQPDGTFCCHFETINKLLVQKWYEQGQVLE
                     RRGLPELTYEAIGIIDAISKLNGEPAMRLAIFNSLSAGEAVDLLPDFGHEIPWWGVW"
BASE COUNT          524 a          906 c          932 g          497 t
ORIGIN      
        1 atgtgatctc gaagtggcac accgtcggac cggacttctc gggggaactc aacgccgtcc
       61 tgtctgagat gggctccacg ccagtcgagg accgcatccc agttcttgcg ttcggctcga
      121 atgcctgccc cgcccagttg cggcacaagg cagagcagtc aggcttccgg ctcgacatcc
      181 ccttgctcga agtcgtggtc gcgggggtca ccgtggggca ctccgcgacg ctttcgtctc
      241 gcgggtactt cccgatgacg ggggtgccgc gctcgtcatc gatcgccagg ggccacctca
      301 tgctgctgga cgagcaccag ttgatggcga tggacaggtc cgagcccggc ttccaacggg
      361 tgctgctgtc tagccgtgac ggctacgcgc tcgagtgcgg cggtgtgcga cttgccacga
      421 cctacatgta tgtcgcccgc gagggtcacc tgatcgacaa ggacaacggc tcgacgctgt
      481 acgacatgga ccacgaagcg ctgctgtcgc ggctcgtgtc atcgccgggg gacttgcggg
      541 cgctgctcgg agacgaccct gaggagatcg tcgcgcgggc caggcgctcc acgtccgggt
      601 ggctgcacac ggtggttgac accctgcacg ctgcgggctg ggtcgagagg cgtgacccag
      661 cgaatctctt ggagtgccac aaggatcgcc ggccgatgta cttcggcgca caggagtctc
      721 cgacacgggt gctttggcac acggagtcgg atatggcgga cagggagcaa aacagcgtct
      781 ccgtttcccc catactgctg tggtaggtaa tcagcgcggc gcgcctctta cgggggtggc
      841 gtgggcgatg ccatcggctt tggcaccagg cgcatagcgc tagtcaccat agaaagttcc
      901 ttccgcaaga agtgtggtag gcttcccgta ttcgtcgtca cagagacgcg cggtggagga
      961 agggcaagtc aatgaggcat ctctcgcaga agcagagcct caccctcaca ggtgggtggg
     1021 gcagcgctgc gtccaggagg gtcgggtagt cgtgggacgc agaggcatgc ggacaatcga
     1081 ctgggccgaa tttgcccgga cgattgacgc cgaaggcgac gccctagcgg cgcttgcgct
     1141 agcgggggag tggctggact ccgctgagcg cggacgagca ctcgcctccg acgacggctc
     1201 caaagtcacc taccgctgga tcaaagccct tgatgtcgcc caatggcacc acaacgaggg
     1261 cgagacgatc tcgggttgcg gccggcgcct tggcgtcacc cgccagcgcg cacagcaact
     1321 cctgtggctc gtcgagtcag aacagaagtt cgacgtgcca gaccgcgccg cagatgacgc
     1381 acgcgctcgc gccagggtgc tggcccgagt gttctcggag tccgtcgcga tcctgcgcca
     1441 cgcccccggc aagacggttc acgaaatggc cgaacagatc tcctgcgacg aagttgacgt
     1501 gtcgctcgtg ttggggccgc tggcggcctt cacgatcaac ccatcgctgt cgacgcgcct
     1561 tgggcgctcg aagtggtccg acgaccagat cgcgtccacg ctgcgggact gctacgccgc
     1621 gaacgacaac acgccgctga cgaagcgggc ctacgcggag ttgaccgcga agaactctcc
     1681 gcctccgccc tcacaggcgc tcatcgccaa gaggttcgga acgtggaacg aagcgctcgt
     1741 gcacgcgggc ctgccgagca acgatcgccc ccacgagtac acatgggccc acactcgcga
     1801 agaggcgctc gggaacatgg tcgagttcgt gctcgcaacg ggcaagacat ccgttggcgc
     1861 ctactccgca tggtccgaga agcgtgatga cgtgatccgg ttcccgcacg ccgttgtcgg
     1921 tgaaggccag tcgtggactg acgccattcg ccaggtcttc gcggtcgtct ctcggccgcc
     1981 gcacgtcgcc gcgtaccgca gtgtgctgct cgctcgcgcg ggagagcagg ccgcggcact
     2041 ggccgggaag ttcgacgcag actttgccgc gagactctaa ctccaatcaa ggaaggggcc
     2101 accatgccca agatgaacgg agccgtcaag gctgcggtag ttattacgct gtgcgcgttc
     2161 gtcgccagcc tcgccttgcc gctcttcgcc ggctaaaccc acaccgcacg tcgcggtctt
     2221 tgtcgcgccc gaaagcggca ggtgcgtgcg tggatacctt tcttggtgag gccaacgacg
     2281 gccccgggaa ctgcgagaga taacttcgcc gtcctggctc aaaagtgtgg tagtcttcca
     2341 cgcatacaga ccagcaacgg taggggagac aggggaacga aatgcacacc gttacagcag
     2401 actttcagga cgaagtgcac gttgaggacc ctgatctcgc tgctctcgtt gagatccgtg
     2461 ctgcatacgg cacgcccgtg gcgatcaacg ccgcagacac cgagcggtgg ttcgtaatct
     2521 cgcgagggaa cttgcgtgac cgtgactaca tccgcttcac cacggcgatg ggtcgcaccg
     2581 tcgagggcac tgcccgcaag cagccggacg gcaccttctg ctgccatttc gagacgatca
     2641 acaagttgct cgtgcagaag tggtacgagc agggccaggt gctcgaacgt cgaggtctcc
     2701 cggaactcac gtacgaggcg atcggcatca tcgacgcgat cagcaagttg aatggcgagc
     2761 ccgctatgcg actcgcgatc ttcaactccc tttccgccgg tgaagccgtc gacttgctgc
     2821 ccgacttcgg ccacgagatc ccctggtggg gcgtatggg
//
LOCUS       DASRKH010000111         3591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_10878, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000111 DASRKH010000000
VERSION     DASRKH010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10878"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VFC06_01890"
     CDS             <1..313
                     /locus_tag="VFC06_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005055244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HZL80683.1"
                     /translation="SHSISAGLDYPGVGPQHAWLADIGRAEYWPVTDADAMEAFRLLS
                     RTEGILPAIESAHALAGAMKLGRELGPDATLLVNLSGRGDKDVETAAHYFDLFDEEPQ
                     Q"
     gene            310..1110
                     /gene="trpA"
                     /locus_tag="VFC06_01895"
     CDS             310..1110
                     /gene="trpA"
                     /locus_tag="VFC06_01895"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HZL80684.1"
                     /translation="MSALTDRLDAIRGEGRSALIGYLPVGFPSVERSVRAMVAMVEAG
                     VDIVEVGLPYSDPVMDGPMIQLAAERALQGGTHVSDAFTAVKAVTDAGAPAVVMSYYN
                     PMLQYGLERFAADLAAAGGSGAITPDLTPDSAGLWLAAARARELDTIFLVAPSSTQER
                     LHMTAAATTGFVYAASLMGVTGERASVGAGAEQLVAATRAAGAQRVCVGLGVSTGAQA
                     ADVARFADGVIVGSALVRCLVEHDNEDAALDALRTKTAELARGVRGDA"
     gene            1107..1973
                     /gene="lgt"
                     /locus_tag="VFC06_01900"
     CDS             1107..1973
                     /gene="lgt"
                     /locus_tag="VFC06_01900"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HZL80685.1"
                     /translation="MIHLSFPSPPVEWAAYQLGPLTIRAYSLWILAGIFIALWLTTKR
                     WVERGGKSEVVSDVAFWAIPFGILGGRLYHLITTPEPYFGAGGHPMNAFKIWEGGLGI
                     WGAVALGSLGAYIGARRHGVKFTAFMDAAAPGLLIAQAIGRMGNYFNQELFGGPTDLP
                     WAVQIDPAFRPEGFEQFTTFHPTFLYEMLWNLAGAALIIYLDRRLDLRGGRVFWLYVM
                     VYVSGRLWIEMVRIDTAVHVLGLRINVWVSIGVLLLGIAMFVMWGRRQAALPRLPREE
                     LAADQREAVVTD"
     gene            2092..>3591
                     /locus_tag="VFC06_01905"
     CDS             2092..>3591
                     /locus_tag="VFC06_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015771503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HZL80686.1"
                     /translation="MATHGGDEPTSGLYDPAYEHDACGVAFVATLRGHAGHDIVDLGL
                     TALKNLEHRGAVGAETETGDGAGILTQVPHEFLQAATDLDLPEQGSYGVGIAFLTPGK
                     EAEEVAAFEAAAVAEQALILGWRDVPVVLDSLGPSARAAAPTFRQVFVVADHLSDIAL
                     DRRLYRVRKRAENNGGPFFASLSARTLTYKGMLTTYQLEQVYPDLLDLLFSSELALVH
                     SRFSTNTFPSWPLAQPLRLIAHNGEINTVRGNRNWIAAREGTLQSDLLGEMKDLMPVC
                     TPSASDTASFDEVLELLHLAGRPLPHAVLMMMPEAWQNNTEMDIERRAFYEYHSALIE
                     PWDGPAALHFTDGTLIGATLDRNGLRPGRFWVTDDGIVVCGSEAGLLDIDPATIVRKG
                     RLQPGRMLLVDTAAGRIVEDDEVKSALAAENPYAQWVADNAVRLAELPDREHVTHSRA
                     SVVRRQRTFGYTEEELKILLEPMAKTAAEPIGAMGSDTPIAVLSNRPKLL"
BASE COUNT          572 a         1180 c         1193 g          646 t
ORIGIN      
        1 ttcgcactcg atttcggccg gtctcgacta cccaggggtg ggcccgcaac atgcgtggct
       61 cgcggacatc gggcgcgccg aatattggcc ggtgacggac gccgacgcga tggaggcctt
      121 ccgcctgttg tcgcgcacgg aaggcattct tcccgcgatc gagtcggcgc acgctctcgc
      181 gggagccatg aagcttggcc gcgagctggg tcccgacgcc accttgctgg tgaacctttc
      241 cgggcgtggc gacaaggacg tggagaccgc tgcccactat ttcgacttgt tcgacgagga
      301 gccccagcag tgagcgcact gaccgaccgg ctcgatgcca ttcgcggcga gggccgctcg
      361 gcgcttatcg gctacctgcc cgtgggcttt ccctccgtcg agcgttccgt acgggccatg
      421 gtcgcgatgg tggaggcggg agtcgacatc gtcgaggtgg gcctgccgta ttccgaccct
      481 gtgatggacg gcccgatgat tcagttggcc gccgagcgtg cgcttcaggg tggcacccac
      541 gtttccgacg cgttcaccgc ggtgaaggcc gtgaccgatg cgggtgcgcc tgccgtggtc
      601 atgtcctact acaacccgat gctgcagtac ggcctggaac gcttcgccgc agaccttgcc
      661 gcggcaggcg gttccggcgc gatcacgccc gacctgacgc ccgattccgc tggcctgtgg
      721 ctcgcagcag ctcgggcgcg cgagctcgac accatcttcc tggtcgcacc gagctcgacg
      781 caggagcgcc tgcacatgac cgccgccgcg accaccggct tcgtctatgc cgcctcgctc
      841 atgggcgtga cgggggagcg cgcctccgtg ggcgccggcg ccgagcagct tgtggcagcg
      901 accagagcgg cgggcgcgca gcgagtgtgt gtcggcctag gcgtatcgac cggggcacag
      961 gcagcagatg tggcgcgctt tgccgacgga gtgattgtgg gctccgcatt ggtgcgctgc
     1021 ctggtggaac acgacaacga agacgcggct ctcgatgcgt tgcgcaccaa aaccgcagag
     1081 cttgctcgcg gagtaagggg tgatgcgtga ttcacctgtc ctttcccagc cctccggtgg
     1141 agtgggccgc ttatcaactc ggcccgctca cgatccgcgc ctactcgcta tggatcctcg
     1201 cgggcatctt catcgccttg tggctcacca cgaaacgatg ggtcgagcgt ggcggcaagt
     1261 ccgaggtagt cagcgatgtc gctttctggg cgatcccgtt cggcatcctc ggcggccggc
     1321 tgtatcacct catcaccacg ccggagccgt attttggcgc gggcggacac cccatgaacg
     1381 ctttcaagat ctgggagggc gggctcggca tctggggagc tgtcgccctt gggtcgctcg
     1441 gcgcctacat cggggcccgt cgccatggcg tgaagttcac cgcgttcatg gatgccgccg
     1501 cgcccggtct ccttatcgcg caagccatcg gccgcatggg caactacttc aaccaagagt
     1561 tgttcggtgg gccgaccgac cttccgtggg ccgtacagat tgatcccgcc tttcgtccgg
     1621 aaggctttga gcagttcacg accttccacc ccacgttcct ttacgaaatg ttgtggaacc
     1681 tcgcgggtgc ggcattgatc atctacctcg atcgacgcct cgatttgcgt ggcggcaggg
     1741 tcttctggct ctacgtgatg gtgtacgtgt ccggccgcct gtggatcgaa atggtgcgca
     1801 tcgatactgc tgtccacgtg ctgggtcttc gcatcaacgt ctgggtgtcg atcggagtct
     1861 tgttgctcgg catcgccatg tttgtgatgt ggggaaggcg ccaagcggct ctcccgcggc
     1921 tgccaagaga ggagctcgcg gcggaccagc gagaagcggt cgttacggac taagggttcg
     1981 ggcgggccta ggtggacgcg gtagtctgac ctttcgtgtg tacaccgggt cgagggcgcc
     2041 ccgaaatagc gaagttcacg gccacgagcc gggacggcaa aggacggtgt gatggccacg
     2101 catggtggtg acgagcccac gtcgggactg tatgacccgg cctacgagca tgacgcgtgt
     2161 ggagtggcct ttgtggcgac gctacgcggt cacgcggggc acgacatcgt cgatctcggc
     2221 ctcactgcgc tcaagaacct agaacaccgc ggcgccgtag gcgccgagac ggaaacgggc
     2281 gacggggcgg gcattctgac gcaggttcct cacgagtttc tgcaggcggc tacagacctc
     2341 gaccttcccg agcaaggctc ctacggcgtc ggcattgcct tcctgacgcc cggcaaggag
     2401 gcggaggagg tggcggcctt cgaggccgcc gcagtcgcgg agcaggcctt gatacttggc
     2461 tggcgcgatg tgccggtcgt gctcgactcg ctcggcccgt ccgctcgtgc cgcagcgccc
     2521 acgttccgcc aggtgtttgt ggtagcggac cacctcagcg acatcgcctt ggaccggcgg
     2581 ctctaccggg tccgcaagcg cgcggaaaac aacggcggac cgttcttcgc ctcgctatcg
     2641 gcgcgcacgc tgacctacaa gggcatgctc accacctacc agctcgagca ggtctacccg
     2701 gaccttctcg acctgctgtt ctcgtcggag ctcgccctgg tccactcgcg tttctctacc
     2761 aacacgttcc cgtcctggcc tctggcccag ccgctgcgcc tcatcgcgca caacggcgag
     2821 atcaacaccg tgcgcggcaa ccggaactgg atcgccgcac gcgaaggaac cctgcagtcc
     2881 gacttgctgg gtgaaatgaa ggacctgatg ccggtgtgca cgccctcggc gtcggacact
     2941 gcatcgttcg acgaggtgct ggagttgctg cacctggccg gtcgcccgct accgcacgcc
     3001 gtgctgatga tgatgccgga ggcgtggcag aacaacaccg agatggacat cgagcgccgc
     3061 gcgttctacg agtaccactc ggcgctcatc gagccgtggg acggccccgc cgcgctgcac
     3121 ttcacggacg gcacactgat cggcgccacc ctggaccgca acggactgcg tccggggcga
     3181 ttctgggtca ccgacgacgg catcgtggtg tgcggatccg aggcgggtct gctggacatc
     3241 gaccctgcca ccatcgtgcg caagggacgc ctgcagcccg gccgcatgct cctggtcgac
     3301 accgctgcgg gacgcatcgt cgaggacgat gaggtgaagt ccgcgcttgc cgccgagaac
     3361 ccctacgccc agtgggtcgc cgacaacgcc gtgaggctgg ccgagctgcc cgaccgcgaa
     3421 cacgtgacgc actcgcgcgc ctcggtggtg cgccgtcagc ggaccttcgg ctacacggaa
     3481 gaagaactca agatcctgct cgagccgatg gcaaagacgg cggcagagcc gatcggcgcc
     3541 atgggatctg acacccccat cgcagtgctc tcgaaccggc caaagctgct g
//
LOCUS       DASRKH010000112         2016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_20311, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000112 DASRKH010000000
VERSION     DASRKH010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20311"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VFC06_01910"
     CDS             complement(<1..530)
                     /locus_tag="VFC06_01910"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HZL80687.1"
                     /translation="MDARTGALRAITTSLTTTAALALVSLPAQAETDHVVGPGDTVSR
                     LAAKYGVPTSSIIAVNGLDARAMIYIGEHLIIPTSSPQPTSVTHVVVRGDTAWDLATA
                     YGVTVSDLSRANSLNVAATIRIGQTLVIPGATASPAAPTRSSASPSAASTAYAVRSGD
                     TLWSIARTFGTSVATTG"
     gene            670..999
                     /locus_tag="VFC06_01915"
     CDS             670..999
                     /locus_tag="VFC06_01915"
                     /inference="COORDINATES: protein motif:HMM:NF037332.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv2175c family DNA-binding protein"
                     /protein_id="HZL80688.1"
                     /translation="MSVPECAEVLGVPLSRVREYLRERYLIAVRRGENNALYLPAGQI
                     IDGEAGPEILATVRGTVMVLADAALSDDVIVEWLMTVNDELGESPLDALRSGKRAPVR
                     RLAQTVF"
     gene            complement(1033..1479)
                     /locus_tag="VFC06_01920"
                     /pseudo
     CDS             complement(1033..1479)
                     /locus_tag="VFC06_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012398383.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(1487..>2016)
                     /locus_tag="VFC06_01925"
     CDS             complement(1487..>2016)
                     /locus_tag="VFC06_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015780200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HZL80689.1"
                     /translation="RIPGMIMISERPAEADDRAVPGHWEGDLILGEGSRSAVGTLVER
                     STRLTVLLHLPDGKSAEQVETAMRKAITAVPSLLARTITWDQGAEMSKHAEFTTKTGI
                     PIYFCDPHSPWQRGTNENTNGLLRQYLPKGTDLSVVNLDELAAIADNLNSRPRKTLGY
                     MTPSEKFAELLASTT"
BASE COUNT          314 a          559 c          752 g          391 t
ORIGIN      
        1 cctgtcgtcg ccaccgaggt gccaaaggtg cgcgcgatcg accacaaggt gtcgccgctg
       61 cggaccgcgt aggcggtgga agccgccgag ggtgaggccg acgagcgggt cggcgcggca
      121 ggggaggcgg tggcgccggg aatcacgaga gtctgcccga tgcggatggt cgcggccacg
      181 ttgagggaat tggcgcggct caggtccgag acggtgacgc catatgcggt ggccaggtcc
      241 caagcggtgt cgccgcgcac gaccacgtga gtgacggagg tgggttgcgg agaagacgtg
      301 gggatgatga ggtgctcgcc gatgtagatc atggcccgcg cgtccaggcc attcaccgcg
      361 atgatgctcg acgtaggcac gccgtacttt gcggcgagcc gtgacaccgt gtctcccggg
      421 cccaccacgt ggtcggtctc cgcttgcgcc ggcagcgaga cgagcgcgag cgcggcggta
      481 gtggtcagtg aggtcgtgat cgccctcaag gctccggtgc gcgcgtccat ggcggctccc
      541 tgcaatcggc gccatcgcga tactcacggt gcgcatgtga ctgatgtgac acatgtgact
      601 gtagctcttc gatccatcct gcacaaggga ccccgagcga atcgctacgc tgggcgtgtg
      661 agtgaatgga tgagtgttcc cgaatgcgcc gaagtactgg gcgtgccgtt gtcccgcgtc
      721 agggagtacc tgcgagagcg atatctgatc gccgtgaggc gtggcgagaa caacgcgctc
      781 tatctccctg ccgggcagat catagatggc gaagcaggac cggagattct tgccacggtg
      841 cgcggcaccg tcatggtgtt ggccgacgcg gcgctgagcg acgacgtgat cgtcgaatgg
      901 ctgatgaccg tgaacgacga actgggggag tcgcccctcg acgcgttgcg atccggcaag
      961 cgcgccccag tgcggcgcct cgcgcagacc gtcttctagc ggcgtccgtc caaaactgac
     1021 cgcgcgggcc gactaggtgt tctggctcat gacgttggtg acgccggttc ggaggcgtga
     1081 ggctgggggt cggtctccga tggcagtgtg gtttcgatgg tagttgtagt ggatgttcca
     1141 gaccttgatc gcgtgggcgc gttgggcttc tgaggtccag atgcgggcgt agaggacctc
     1201 ggcggccagg gtccggttgt agcgttcgac tttgccgttg tggcggggtg tgtaggggcg
     1261 aatgtattgg tggcgggacg cggtcgcagc gatcacccgt gtgaagtctt tggcccggta
     1321 gttcgcaccg ttatcggtca cgaccgtgac gacccgttcg atgccgtggg cacggaagaa
     1381 gacgcgggcc cgggcgtaga acccgatcgt ggtggaggcc tgctcgttgg gtaggtgttc
     1441 ggtgtaggcc agacgtgagt agccgtcaac gatcgagtgg gcggattcaa gtggtggagg
     1501 caaggagttc tgcgaacttc tctgatggtg tcatatagcc gagtgtcttg cggggtcggg
     1561 agttgaggtt gtctgcgatg gcggctagtt cgtcgaggtt gacgacgctc agatcggtgc
     1621 ctttgggcag gtactggcgc agcagcccgt tggtgttctc gttggtgccg cgctgccatg
     1681 gcgagtgggg gtcgcagaag tagatcggta tcccggtttt cgtggtgaac tcagcgtgct
     1741 tggacatctc cgcgccctgg tcccacgtga tcgtgcgggc cagcaaggac ggcactgcgg
     1801 tgatcgcctt gcgcatcgcg gtctcgacct gttcggcgct cttgccgtcc ggcaggtgga
     1861 gcagcacagt caggcgcgtg gagcgctcca cgagcgtccc gacagcgctg cggcttcctt
     1921 cgccgaggat caggtcacct tcccagtgcc cgggaacggc acgatcgtcg gcctcggcgg
     1981 gacgttcgct gatcatgatc atgcccggga tgcggc
//
LOCUS       DASRKH010000113         2434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_23444, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000113 DASRKH010000000
VERSION     DASRKH010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23444"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="VFC06_01930"
     CDS             complement(<1..695)
                     /locus_tag="VFC06_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HZL80690.1"
                     /translation="MSDQPVSVIPLLHALASTGGSDLHCKVGSAPRIRVDGRLRKLQV
                     PPLTAEDTRNMVAQVLPEDLVAQFRDIHEADFAFSLSGVGRFRVNTYVARGTDCLVFR
                     RVAVGAQPMAELGIPEVVADLALEPRGLVLVTGPTGSGKTTTLASMVDLINTFREVNI
                     ITIEDPIEVLHSDKKSIISQREVRSDTLDFMNALRGAMRQDPDVILVGEMRDIETVRA
                     ALSAAETGHLVLAT"
     gene            complement(1025..1270)
                     /gene="rsrA"
                     /locus_tag="VFC06_01935"
     CDS             complement(1025..1270)
                     /gene="rsrA"
                     /locus_tag="VFC06_01935"
                     /inference="COORDINATES: protein motif:HMM:TIGR03988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol system anti-sigma-R factor"
                     /protein_id="HZL80691.1"
                     /translation="MPKRQCQEALDKLFEFIDGELSDDDLLRIGAHLKECPPCEAEHQ
                     INEKIRLLVSQAHGEKAPHQLRERVLSTLKMARDEYK"
     gene            complement(1342..2019)
                     /locus_tag="VFC06_01940"
     CDS             complement(1342..2019)
                     /locus_tag="VFC06_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZL80692.1"
                     /translation="MTMLAERPITLGTARPAQRVFPGLGRRPVGSAPMTTHTDGFDFE
                     TDALSFMDQLYAAALRMTRNSADAEDLVQETYAKAFAGRDKFTPGTNLKAWLYRIQTN
                     AFINTYRKKQREPKRTDSENVEDWQLAAAAEHQSAGLASAEQLALDSIGDDDVRNALS
                     ELSGDFRMAVFLSDVEGFAYKEIAEIMGTPVGTVMSRLHRGRRMLREKLRDYAAERGI
                     YDHRAGS"
     gene            2201..>2434
                     /locus_tag="VFC06_01945"
     CDS             2201..>2434
                     /locus_tag="VFC06_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80693.1"
                     /translation="MGPLLKDAGVKVPVETLVRVHSAATAATAAVLAISKSPRSAGVA
                     LTGLAAVTVATSVPFWRLPDGEERDAALNEFMKN"
BASE COUNT          414 a          770 c          772 g          478 t
ORIGIN      
        1 gtggcgagca cgaggtgtcc ggtctcagct gcggacaacg cggctcgcac ggtctcgatg
       61 tcacgcatct cacccacgag aatcacatcg gggtcttgac gcatggctcc gcgcagggcg
      121 ttcatgaaat ctagggtgtc cgagcgcacc tcgcgctggc tgatgatcga cttcttgtcg
      181 ctgtggagta cctcgatcgg gtcctcgatc gtgatgatgt tgacctcgcg gaacgtgttg
      241 atgaggtcaa ccatcgacgc caacgtggtg gtctttccgg atccggtggg ccccgtgacg
      301 agcaccagtc cgcgcggctc gagcgcgagg tccgcaacca cctcgggaat acccagctct
      361 gccatgggct gtgcgcccac ggcgactcgg cggaacacca ggcagtctgt tccgcgcgcc
      421 acgtaggtgt tgacgcggaa gcgtcccact ccggacaggg agaacgcgaa gtcagcctca
      481 tggatatcgc ggaactgcgc caccaggtcc tcgggcagga cttgggccac catgttgcgc
      541 gtgtcttccg ccgtcagcgg cggcacctgc aacttgcgca gacggccgtc gacgcggatg
      601 cgcggcgcgg acccgacctt gcagtgaagg tcggagcctc ccgtggacgc gagggcgtgc
      661 aggaggggga taacggatac gggctggtcg ctcacgtttg ctccatcggc atcacgggga
      721 cttgacttga gaaggctaac gcgttgagtg tatcgatgcc tccgcgacgc ctctcgaagg
      781 cggacgatgt ggtttggcgc ttcgtgtgga atgatggagg gtcgctcgaa cgaaacccaa
      841 ggagtcacca tgagcaagcg aggccgcaag cgcaagaccc gcgcgaagtc cgccgccaat
      901 cacggcaagc gtcccaacgc ttaattcgtg cggggctttc aggtcctgag ataacaaagc
      961 ccgcgtcaat cgacgcgggc tttgtttgtg gtgctcgcgc acaagcgcgg caacgacact
     1021 cctgctactt gtactcgtct cgagccatct tgagggtgct gagcactcgc tcacgcaact
     1081 ggtgaggcgc cttttctccg tgcgcttgag acacgagcaa cctgatcttc tcgttgatct
     1141 ggtgctcggc ctcgcacggc gggcactcct tgaggtgtgc gccgatgcgc aacaagtcat
     1201 cgtcggacaa ctcgccgtca atgaactcga atagtttgtc gagagcctct tggcattgcc
     1261 gtttaggcac ggtgcacctc catcactgac gcacccccgg cgcaccaacg cgccgtcgtc
     1321 ttcatctgat ttcccatggt cttacgaccc tgccctgtgg tcgtagattc cacgttcggc
     1381 cgcgtagtcg cgcagcttct cgcggagcat ccggcggccg cggtgcagcc gggacatcac
     1441 agtgcctacg ggggtgccca tgatctcagc gatctccttg taggcgaagc cttcgacgtc
     1501 gctcaagaac acggccatcc ggaagtcccc cgacagctcc gagagcgcat tgcgcacgtc
     1561 gtcgtcaccg atgctgtcca gagccagttg ctcggccgat gccagcccgg cggattggtg
     1621 ctctgccgcg gcggccaact gccagtcctc gacgttttcg gaatcggtgc gcttcggttc
     1681 gcgctgcttc ttgcggtacg tgttgatgaa cgcgttggtc tggatgcgat acagccacgc
     1741 cttgaggttg gtgccgggcg tgaacttgtc ccgaccggcg aacgccttgg catacgtttc
     1801 ctgcaccagg tcttcggcgt ctgcgctgtt gcgcgtcatt ctgagcgcgg cggcatacaa
     1861 ctggtccatg aaggacaggg catcggtctc gaagtcaaaa ccgtcggtgt gggtggtcat
     1921 tggggctgag cctaccgggc gccggcccaa gccaggaaag acgcgctgcg cgggacgtgc
     1981 agtccctagc gtgatcggcc tttccgcaag catcgtcatg cctgtcagaa cgcgagacga
     2041 cgggtcctca ttccccatcg tgtacatcgg ctcgcacttg ggtaggctgg gaagcatgat
     2101 gcgtgcacta gctcgtccct tgttggcctc ctggtttgtc tacgggggtg tccgcgacgc
     2161 tttggaaccg caagcgcgaa ctgctgcggc tgagcccatc gtgggacctc tcctgaagga
     2221 cgctggcgtc aaggtgccgg tggagaccct tgtcagggtc cacagcgccg ccaccgccgc
     2281 taccgccgcc gtgctggcca tctccaagag cccgcgctcc gcaggagtgg ccctcaccgg
     2341 ccttgccgca gtcaccgtgg ccaccagcgt gcccttctgg cggcttcctg atggcgaaga
     2401 gcgcgacgcc gccctgaacg agttcatgaa gaac
//
LOCUS       DASRKH010000114         2240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_25090, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000114 DASRKH010000000
VERSION     DASRKH010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25090"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1965
                     /locus_tag="VFC06_01950"
     CDS             <1..1965
                     /locus_tag="VFC06_01950"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HZL80694.1"
                     /translation="LLGVSLAVAAVPEGLPTILTVVLALGVQRMAAHHAIVKDLSSVE
                     TLGSASVICSDKTGTLTKGEMTIGCVVTASGEVTVTGAGYRPDGQVVHDGVPLTAGSD
                     LWNETALVLGGGSLANNATLREEDGEWVIQGDPTEAAFMVAEIKLGTQDDRTGRFQRQ
                     GEIPFTSERKMMTSLEADAQRAGRLAVVTKGAPDVLLDRCSHVQVGDRHELLDIAGRA
                     RILRDVERLSDEAFRTLGVAYRPLDVTTPPALDDSLEQGLVYAGTAGIIDPPRPEAAV
                     AIAEAHRAGVRVIMITGDHPRTASRIARDLGIVEREHVAVTGVELDRLDADQLRETVR
                     YRSVYARVTPRHKLRIVAALQADHQVVAMTGDGVNDAPALKAADIGVAMGLTGTEVTK
                     EAANMILADDNFATIVQAVREGRGIFLNIKKFLRYLLSSNMGEVLTVFFGVVLAGVLG
                     LAHVDGTVALPLLATQILWINLLTDSAPALAMGVDTETEDVMSRPPRSLQDRAIDGRM
                     WVGVLLIGLVMATVTLLTIDLFVAGGLIEGTHSLNNARTAGFTVLVLAQLFNALNARS
                     ETATAFHRLFANAWLWGAIALSTVLQVAVVHLPVLNEAFTTAPLSLNQWLVCVAMAST
                     VLWVSEIRKLVLRLVDRRSHQRALVEERAA"
     gene            1962..>2240
                     /locus_tag="VFC06_01955"
     CDS             1962..>2240
                     /locus_tag="VFC06_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010550114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HZL80695.1"
                     /translation="MTSRAVQGGNQVDRLLFRFMLLSLAAAVVTIALKVGAAVITGSV
                     GFLSDAMESGVNLVAAVVALFALRVAARPSDASHHFGHGKAEYLSALVE"
BASE COUNT          323 a          826 c          757 g          334 t
ORIGIN      
        1 ctgctggggg tctcccttgc agttgccgcg gtgcccgagg gtctgcccac gatcctcacc
       61 gtcgtcctcg cgctcggcgt gcagcggatg gcggcccacc acgcgatcgt caaggacctc
      121 tcctcggtcg agaccctggg ctcggcgtcg gtgatctgct cggacaagac cgggaccctg
      181 accaagggcg agatgaccat cgggtgcgtg gtcaccgcgt cgggcgaggt cacggtgacg
      241 ggcgccgggt accggcctga cggccaggtc gtccacgacg gcgtccccct gacggcgggg
      301 agcgacctgt ggaacgagac cgccctcgtg ctcggcggcg gcagcctggc gaacaacgcc
      361 acgctgcggg aggaggacgg cgagtgggtc atccagggag accccaccga ggccgccttc
      421 atggtcgccg agatcaagct cggcacgcag gacgaccgga cgggacggtt ccagcgacag
      481 ggtgagatcc cgttcacctc cgagcgcaag atgatgacca gcctcgaggc cgacgcccag
      541 cgcgcgggcc ggctcgcggt ggtcaccaag ggcgccccgg acgtcctgct cgaccggtgc
      601 tcgcacgtgc aggtcggcga ccggcacgag ctgctcgaca tcgcaggcag ggcgcggatc
      661 ctccgcgacg tggagcgcct gtccgacgag gccttccgca cgttgggcgt ggcctaccgg
      721 ccgctcgacg tcaccacccc accggcactc gacgactccc tggagcaggg cctcgtctac
      781 gcgggcacgg cggggatcat cgatccgccc cgccctgagg cggccgtggc catcgccgag
      841 gcccatcgcg ccggggtccg ggtcatcatg atcaccggtg accatccccg gactgcgagc
      901 cgcatcgccc gcgacctcgg catcgtcgag cgcgagcacg tggcggtgac cggtgtcgag
      961 ctggaccgcc tcgacgcgga ccagctccgg gagaccgtcc gctaccggtc cgtgtacgcc
     1021 cgggtgaccc cgcggcacaa gctgcgcatc gtcgccgcgc tccaggccga ccaccaggtc
     1081 gtggccatga ccggagacgg cgtcaacgac gcgccggccc tgaaggccgc cgacatcggc
     1141 gtcgcgatgg gtctgaccgg caccgaggtc accaaggaag cggcgaacat gatcctcgcc
     1201 gacgacaact tcgcgaccat cgtccaggcc gtccgcgagg gccgcggcat cttcctgaac
     1261 atcaagaagt tcctgcgcta cctgctgtcg tcgaacatgg gcgaggtcct gacggtcttc
     1321 ttcggcgtcg tcctcgccgg ggtcctcggg ctggcccacg tcgacggcac ggtcgcgctc
     1381 cccctgctgg cgacccagat cctgtggatc aacctgctca ccgacagcgc acccgccctg
     1441 gccatgggcg tcgacaccga gaccgaggac gtgatgagcc gcccgccgcg gtcgctccag
     1501 gaccgcgcca tcgacggccg catgtgggtc ggcgtcctcc tcatcggtct cgtcatggcg
     1561 acagtcacgc tgctcaccat cgacctgttc gtggccgggg gtctcatcga gggcacccat
     1621 tcgctgaaca acgcgcggac agcaggcttc acggtcctcg tgctcgcgca gctgttcaac
     1681 gccctcaacg ctcgctccga gaccgcgacc gccttccacc ggctgttcgc gaacgcgtgg
     1741 ctgtggggag ccatcgcgct gtcgacggtg ctccaggtcg ccgtcgtcca cctgcccgtc
     1801 ctcaacgagg cgttcacgac cgcgccgctc tccctcaacc agtggctcgt ctgcgtggcg
     1861 atggcgagca cggtgctgtg ggtcagcgag atccggaagc tcgtcctgcg actcgtcgac
     1921 cggcgcagcc accagcgggc actcgtcgag gagcgcgcag catgacgagc cgggccgtcc
     1981 agggcggcaa ccaggtggac cggctgctct tccggttcat gctgctctcg ctggcggcgg
     2041 ccgtcgtgac catcgccctc aaggtcggcg ccgccgtcat caccgggtcg gtcgggttcc
     2101 tgtccgacgc catggagtcc ggcgtcaacc tcgtcgccgc ggtcgtggcg ctgttcgcgc
     2161 tgcgcgtggc cgcccgcccg tccgacgcga gccaccactt cggtcacggc aaggccgagt
     2221 acctgtccgc tctcgtcgag
//
LOCUS       DASRKH010000115         2612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_9967, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000115 DASRKH010000000
VERSION     DASRKH010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9967"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="VFC06_01960"
     CDS             <1..674
                     /locus_tag="VFC06_01960"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HZL80696.1"
                     /translation="DFPAPRFVAHGLPSQGYPWPWAVVTWVPGQTADAVPLYADAGAA
                     VGRALAQVHTPSPDDAPFNTEQSIPMADRDPKVRERVTALAQAPGPSGETMDVAAALA
                     IWDAALAAREHTEWVWSHADFHGANVLSHDGAFGGIVDWGSMAMCDPAVDLGFVHTLM
                     PRAGVDAAFAEYGRLTGRVDDDFLARARGIALSKCVGVAMSPRPVTTAMGWRGLVALG
                     VASTA"
     gene            727..2022
                     /locus_tag="VFC06_01965"
     CDS             727..2022
                     /locus_tag="VFC06_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZL80697.1"
                     /translation="MASRTAWLVFATASAAYFMAVLHRTAFGVAGVQALDRFGIEATA
                     LSLLAVIQITMYAAMQVPAGNLLDRFGPAAVIVAGSLVMAVGQAMMAFAPNYGWALAA
                     RLLIGAGDAPVFIAAARLVAHHFPPRRVPVMVQITGLIGQAGQLATAIPLAFVLHWAG
                     WMPAFGSLAIVGAVVAVAVWSVLIRPGNASFSPERSASISVRQAMATSMRTPGVRLGF
                     WSHFLGPFSANTVALLWGFPYFTTAQGRSTAEASLLLTCMVIASITVAPIIGTLTGRH
                     PLRRTWLVLGGAGVVALAWVAILVPTTPRPLWQLIAFAFVVGAGGPMSLIGMDFAHTY
                     AARERLGSATGFVNMGGFVSTIISVLMVGVLLQSVSPAGVSEYSLGEYRVAFAALAVP
                     WVIGVAGVFISRRDTRAVMAADGVAVPTVRDVWRRYRAR"
     gene            complement(2143..>2612)
                     /locus_tag="VFC06_01970"
     CDS             complement(2143..>2612)
                     /locus_tag="VFC06_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HZL80698.1"
                     /translation="NTDANSTCLAAMSPRGDMADKHVELASVLVESAIASGARLIVVG
                     GFSSLRPAPGAPRFIEGDVPEEYRSEAQAGHAVLELLQAADPALDWTFVSPAATFGAF
                     APGEATGIYRLGGDVAMFDEDGKSYISAPDFAKAILDMIDGNEHHREHVSVIG"
BASE COUNT          354 a          936 c          879 g          443 t
ORIGIN      
        1 gggactttcc ggcgccccgc tttgtcgcgc acggcctgcc aagccagggc tacccgtggc
       61 cgtgggccgt cgtcacctgg gttccgggtc agacggccga tgccgtcccc ttatatgccg
      121 acgcgggggc ggccgttggc agggccctgg cccaggtgca caccccctct ccggatgacg
      181 cgccgttcaa caccgagcaa tccatcccga tggcggatcg cgacccgaag gtccgcgagc
      241 gcgtgaccgc gctcgcccag gcgccggggc cgagcgggga gacgatggac gtggccgcgg
      301 cactcgcgat ctgggatgcc gcgctcgccg cgcgcgagca caccgagtgg gtctggtcgc
      361 acgcggactt ccacggcgcc aacgtcctga gccacgacgg cgcgttcggt ggcatcgtcg
      421 actggggctc gatggccatg tgcgacccgg ccgtggacct cggattcgtc cacaccttga
      481 tgccgcgggc gggcgttgac gccgccttcg ctgaatatgg ccgcctcacc gggcgagtag
      541 acgacgactt cctcgcccgc gcacgcggca tcgcgctcag caaatgcgtc ggcgtcgcca
      601 tgtcccctcg acccgtcacc acagcgatgg gctggcgcgg actcgtggcg cttggggtag
      661 cttccaccgc gtagcctgag tcttccggac accataagga ccctcgtgcg caagactcgc
      721 cctcccatgg ccagccgtac ggcatggctc gtattcgcca ccgcctcggc cgcctacttc
      781 atggctgtgc tgcaccgcac cgccttcggc gtggccggcg tccaggcgct cgaccgcttt
      841 ggcatcgagg ccaccgcgct gtcgctgttg gccgtcatcc agatcacgat gtacgcggcg
      901 atgcaggtcc ccgcagggaa tctgctggat cgcttcggac ccgcggccgt gattgtggcc
      961 ggatcgctcg tcatggccgt gggccaggcc atgatggcgt tcgcccccaa ctacggctgg
     1021 gcacttgcgg cgcgcctgct gattggcgcg ggagacgccc cggtcttcat cgccgccgcg
     1081 cgcctcgtcg cccaccattt cccgccgcgg cgagtaccag tcatggtgca gatcaccggg
     1141 ctgatcggtc aggctgggca actcgcaacg gccatccccc tggcgtttgt gctgcactgg
     1201 gccgggtgga tgcccgcgtt cggctcactg gccatcgtgg gcgcggtcgt cgccgtcgct
     1261 gtgtggtcgg tgctcatccg ccccgggaat gcctccttct caccggaacg ctccgcgtcg
     1321 atctcggtgc ggcaggccat ggccacgtcg atgcgcacgc ccggcgtacg gctcggattc
     1381 tggagccact tcctggggcc cttctccgcc aatacggtcg cgctgctgtg ggggttcccc
     1441 tacttcacca ctgcccaggg ccgctccacg gcggaggcca gcctgctgct gacctgcatg
     1501 gtgatcgcga gcatcaccgt ggcgccgatc atcggaactc tcacgggccg ccacccattg
     1561 cgacgcacct ggctggtgct cggcggagcg ggggtcgtgg cgctcgcatg ggtcgcgatc
     1621 ctggtgccca cgacaccgcg gccgctgtgg cagctcatcg cgttcgcctt cgtggtgggc
     1681 gcgggcggcc cgatgtcgct catcggcatg gatttcgcgc acacctacgc ggcgcgcgag
     1741 cggctgggca gcgccaccgg attcgtcaac atgggtggat tcgtctcgac catcatcagc
     1801 gtgctcatgg tgggcgttct gctccagtcg gtgtcccccg ctggtgtctc cgaatactcg
     1861 ctcggcgagt accgcgtcgc gttcgccgcg ttggcggtcc cgtgggtcat cggcgtggcg
     1921 ggggtcttca tcagccgacg cgacaccagg gccgtcatgg cggccgacgg cgtggccgtg
     1981 cctaccgtgc gcgacgtctg gcggcgctac cgggctcgtt gagccctccg aactgcgtgg
     2041 acaccgtcgc gccagcaccg cgaatccgca ttgcgccccc atcgcctgcg ggcgaagcaa
     2101 acaccatgtc gattggcggt gctcacgcga cacctcgacg cgttagccga tgacggagac
     2161 gtgctcccgg tggtgctcgt tgccgtcgat catgtcgagg atcgccttgg caaagtcggg
     2221 cgcggagatg taggacttgc cgtcctcgtc gaacatcgcg acgtcgccgc caagacggta
     2281 gatgccggtc gcctcgccgg gcgcgaacgc gccaaatgtc gctgccgggc tcacgaaggt
     2341 ccagtccaag gcagggtccg cggcctgcag caactcgagg accgcatgac cggcctgcgc
     2401 ctcggaacgg tactcctcag gaacgtcgcc ctcgatgaac cgcggggcgc ctggcgccgg
     2461 ccgcagggac gagaacccac ccaccacgat caggcgcgcg cccgacgcaa tggcggactc
     2521 caccaacacc gacgccaact ccacgtgctt gtccgccatg tcgccgcgcg gcgacatggc
     2581 ggccaagcac gtggagttgg cgtcggtgtt gg
//
LOCUS       DASRKH010000116         2548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_36439, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000116 DASRKH010000000
VERSION     DASRKH010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36439"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..682)
                     /locus_tag="VFC06_01975"
     CDS             complement(14..682)
                     /locus_tag="VFC06_01975"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HZL80699.1"
                     /translation="MTVTLVRETTLWDAGARLVAGVDEVGRGALAGPVSVGVAVLKRC
                     TTWPTGLADSKELSPKAREALATVIPDFGVGRAVGHASAAEIDEHGIIAALRLAGTRA
                     LASLASDGIVPDAILLDGRHDWLTPPVADLFSDDAETAAIAPVTMVVKGDRLCASIAA
                     ASIVAKVERDELMRAAHASHPAYGWEGNKGYGAAGHMAALRELGPSPLHRVSWNLPEQ
                     VGVS"
     gene            complement(705..1346)
                     /gene="lepB"
                     /locus_tag="VFC06_01980"
     CDS             complement(705..1346)
                     /gene="lepB"
                     /locus_tag="VFC06_01980"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HZL80700.1"
                     /translation="MWGWIREILIVLGSALILSLLLKTFVFQSFWIPSGSMENTLQVR
                     DRILVSLWRPGPLDLRHGDVVVFRDPGGWLGPTAEPAPTGLAKGWSEFATFVGLLPQD
                     AGEHLVKRVIGLPGDTVACNTADDKVTVNGVELVENYIKPGTESCTHAWEVIVPEDRV
                     WVLGDNRSNSADSRAHLGDPGGGMVPIDNVVGTAFVTVWPLDHWRMLDNPYPG"
     gene            complement(1406..1753)
                     /gene="rplS"
                     /locus_tag="VFC06_01985"
     CDS             complement(1406..1753)
                     /gene="rplS"
                     /locus_tag="VFC06_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HZL80701.1"
                     /translation="MNKLDGVNATQLRDDIPAFRAGDTLKVHVKVVEGTRSRIQMFQG
                     IVIARQGTGVGATFTVRKVSFGVGVERMFPLHAPTIDKIEVDRRGDVRRAKLYYLRGL
                     RGKAARIREKREN"
     gene            complement(1912..>2548)
                     /gene="trmD"
                     /locus_tag="VFC06_01990"
     CDS             complement(1912..>2548)
                     /gene="trmD"
                     /locus_tag="VFC06_01990"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HZL80702.1"
                     /translation="LGRAREAELVQANVHDLREWAAIKADGTPDVHRTVDDSPFGGGA
                     GMVMKADVWGRALDDVLRPGGVLVIPSPAGIPFTQALAADLAQADQIAFACGRYEGID
                     ARVGEHYEAAGFRVLELSLGDYVLNGGEVAAFAMIEAITRLIPGFMGNAESIVEESHS
                     DGLLEYPSYTRPASWRGLEVPDVLLSGHHGKIESWRREQQVKRTRERRPDL"
BASE COUNT          429 a          877 c          823 g          419 t
ORIGIN      
        1 aactgaggcg cgttcagctc acgccgacct gctcgggcag gttccacgac accctgtgca
       61 gggggctcgg gccgagttcc cgcaaggccg ccatgtgccc cgcggcgccg tagcccttgt
      121 tgccctccca cccgtaagcg gggtgcgacg cgtgcgccgc gcgcatgagt tcgtcgcgct
      181 ccaccttggc gacaatcgag gctgccgcga tcgacgcaca cagccggtcg cccttgacga
      241 ccatggtgac gggagcgatg gccgcagtct cggcatcgtc cgagaacagg tctgcgacag
      301 gcggggtcag ccagtcgtgg cggccgtcga gcaggatcgc gtcgggcacg atgccgtcgg
      361 acgccagaga ggcaagggcg cgagtccccg ccagacgcag cgcggcgatg atgccgtgtt
      421 cgtcgatctc cgcagcgctc gcatgcccga ccgcgcggcc cacgccaaaa tcggggatca
      481 ccgtcgcgag cgcctcgcgc gccttggggc tcagttcctt ggagtccgcg agccccgtgg
      541 gccacgtggt gcaccgcttc agtacggcaa cgccgacgct cacgggaccg gcgagcgctc
      601 ccctgcccac ctcgtcgacg cctgcgacca ggcgcgcgcc cgcatcccac aaagtggtct
      661 cgcgaacaag ggtgacagtc atgcctcgac cctaagggtg ccagctagcc ggggtaggga
      721 ttgtcgagca tcctccagtg atccaacggc cataccgtca cgaacgctgt gcccacgacg
      781 ttgtcgatcg gcaccatgcc gccgccgggg tcccccaggt gggcgcgcga gtcggccgag
      841 ttggaacggt tgtcgcccag cacccagact cggtcctcgg gaacgatgac ctcccacgcg
      901 tgggtgcagc tttcggtgcc cggcttgatg tagttctcga cgagttcgac gccgttgacg
      961 gtgaccttgt cgtcggcggt attgcacgcc acggtgtctc ccggcaggcc gatgacccgc
     1021 ttcacgaggt gctcgcccgc gtcttgaggc agcaggccca caaacgtggc gaactcgctc
     1081 caacccttgg caagacccgt aggtgccggc tccgctgtcg ggccaagcca gccgcccggg
     1141 tcgcggaaca ccacaacgtc gccatgacgg aggtcgagcg gtccgggtcg ccacagcgac
     1201 accagaatgc ggtccctcac ctggagggtg ttctccatcg acccggaggg gatccagaag
     1261 gattgaaaga cgaaggtctt gagcagcagc gacaggataa gcgcgctgcc cagcacgatc
     1321 aggatctcgc ggatccaccc ccacacacga cgaccggcgg aaaggtcaga cctttccgcc
     1381 ggtgcgacgt gtgaaggaga aatgactagt tctcgcgctt ctcgcgaata cgtgctgcct
     1441 tgccgcgcag gccgcgcagg tagtacagct tggcgcgacg cacgtcgccg cgacggtcga
     1501 cctcgatctt gtcgatggtc ggtgcgtgca gggggaacat gcgctccacc cccacaccaa
     1561 aggaaacctt gcggactgtg aaggtcgcgc cgacgccggt tccctggcgg gcgatcacga
     1621 tgccctggaa catctggatg cgtgagcggg tgccttcgac aaccttcacg tgaaccttga
     1681 gcgtgtcacc ggcacggaat gccgggatgt cgtcgcgaag ctgtgtggcg ttgacgccgt
     1741 cgagcttatt catgtgtcac tcctcgcttc tgccacaggt catccacgat ctcttgcctt
     1801 gcggcactcg ataaaggatt gtgcgctgcg ggaaccgctg aaccccctgt ggcaggtctc
     1861 agctccgcaa gcactaacga actattgtgc cacaccgtcg tcacggtcgg ctcacaggtc
     1921 cggccggcgc tcgcgggtgc gcttgacctg ctgttcgcgc cgccacgact cgatcttgcc
     1981 gtggtgaccg ctgagcaaca catcaggcac ctcgagacct cgccacgacg caggccgagt
     2041 gtatgagggg tactcgagaa gtccgtcgga gtggctttcc tcgacgatgc tctcggcgtt
     2101 gcccatgaag cccgggatga gcctggtgat ggcctcgatc atcgcgaacg cggccacctc
     2161 tcccccgttg agcacgtagt cgccgagcga gagctccaag acccggaatc ccgccgcctc
     2221 gtagtgctcg cccacgcgcg cgtcgatgcc ttcgtagcgg ccgcacgcga acgcgatctg
     2281 atccgcctgg gcaagatcgg ccgcaagcgc ctgggtgaag gggattcctg cgggactggg
     2341 gatgacgagc acgccgcccg gccgcagcac gtcgtcaagc gcgcgccccc acacgtcggc
     2401 cttcatcacc atgccagctc cccctccgaa gggggaatcg tccacggtgc ggtgaacatc
     2461 gggcgtgccg tcggccttga tggcggccca ctcgcgcaga tcgtgcacgt tcgcctggac
     2521 cagctcggct tcgcgggccc tgcctagc
//
LOCUS       DASRKH010000117         2390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_31107, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000117 DASRKH010000000
VERSION     DASRKH010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31107"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1436)
                     /gene="purH"
                     /locus_tag="VFC06_01995"
     CDS             complement(<1..1436)
                     /gene="purH"
                     /locus_tag="VFC06_01995"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015770970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HZL80703.1"
                     /translation="MTYTPIRRALVSVFDKTGLEALARGLHAAGVTIVSTGSTAATIA
                     AAGVPVTEVATLTGFPECLDGRVKTLHPRIHAGILADRTNPGHVAELEALGVEAFDLV
                     VVNLYPFVDTVASGASVAECIEKIDIGGPSMVRAAAKNHGSVAVVVDPAAYPHVLEAT
                     TAGGFTADERRLLAMAAFRHTASYDVAVATWFAEQFDDAETPAWRGETWTLAHTLRYG
                     ENPHQRAAVYLTAGAQGLAQAEQLGGKEMSYNNYQDTDAAIRAAYDHARPAVAIIKHA
                     NPCGIATGTDIADAHAKAHACDPVSAYGGVIAANGTLTLAAAEQIAPVFTEVVIAPDF
                     EPAALELLSKKKNIRLLTVTSAPPRAFELRPVSGGVLAQQTDRIDAAGDDPATWTLAT
                     GEAVDAATLADLAFAWRSVRAVKSNAILLASGGASVGVGMGQVNRVDSCRLAVERAGD
                     RARGSVAASDAFFPFADGLQVLLDAGVRA"
     regulatory      complement(1459..1544)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01750"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ZMP/ZTP riboswitches appear to regulate genes
                     involved in one-carbon metabolism; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="5-aminoimidazole-4-carboxamide
                     ribonucleotide (ZMP)/
                     5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl
                     5'-triphosphate (ZMT)"
                     /db_xref="RFAM:RF01750"
     gene            complement(1548..2174)
                     /gene="purN"
                     /locus_tag="VFC06_02000"
     CDS             complement(1548..2174)
                     /gene="purN"
                     /locus_tag="VFC06_02000"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HZL80704.1"
                     /translation="MQQQFRVVVLVSGGGSNLAALLDASRDPAWGATIVGVGADRPGT
                     GGVGIARAAGIPTFELAVRDLPTRADWDAALTDAVAALRPDLVVSAGFLKLCGPVFLE
                     RFGDRYINTHNALLPSFPGIHGPRDALAYGVKLTGATLFLVDAGVDTGPILAQVAVPV
                     LADDDEETLTERIKVAERAQLVESVGRLAREGWRVDGRRVSFGGRATP"
     gene            complement(2184..>2390)
                     /locus_tag="VFC06_02005"
     CDS             complement(2184..>2390)
                     /locus_tag="VFC06_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80705.1"
                     /translation="VDDTVTSVPDDDALPLADAAPAPATDPTATDPTAAIDPTAATAV
                     TSPTAASAAGAGPGRTLLVEDPRR"
BASE COUNT          311 a          839 c          924 g          316 t
ORIGIN      
        1 gcccggacgc ccgcgtcgag gaggacctgc aggccgtccg cgaacgggaa gaacgcatcc
       61 gacgccgcga ccgagccgcg cgcgcggtca ccggcccgct cgactgccag ccggcacgag
      121 tcgacgcggt tgacctgccc catcccgacc ccgaccgacg caccacccga ggcgagcagg
      181 atcgcgttgg acttcaccgc gcgcaccgac cgccacgcga acgcgaggtc ggcgagcgtc
      241 gcggcgtcga ccgcctcgcc ggtcgcgagc gtccacgtcg ccgggtcgtc gccggccgcg
      301 tcgatccggt cggtctgctg cgcgaggaca ccacctgaga cgggtcgcag ctcgaacgcg
      361 cgcggcggcg cggacgtgac ggtcagcagg cggatgttct tcttcttgct gagcagctcg
      421 agcgcggccg gctcgaagtc cggcgcgatg acgacctcgg tgaacaccgg cgcgatctgc
      481 tcggccgcgg cgagcgtgag ggtgccgttc gccgcgatca cgccgccgta cgcggacacg
      541 gggtcgcacg cgtgggcctt ggcgtgcgcg tcggcgatgt cggtcccggt cgcgatgccg
      601 cacgggttcg cgtgcttgat gatcgcgacg gcggggcggg cgtggtcgta ggcggcgcgg
      661 atcgcggcgt ccgtgtcctg gtagttgttg tagctcatct ccttgccgcc gagctgttcg
      721 gcctgcgcga ggccctgcgc gcccgcggtg aggtagacgg cggcccgctg gtgggggttc
      781 tccccgtacc gcagcgtgtg ggcgagcgtc caggtctcgc cacgccaggc cggagtctcg
      841 gcgtcgtcga actgctcggc gaaccaggtg gcgaccgcga cgtcgtagga cgcggtgtgg
      901 cggaacgccg ccatcgcgag cagccgccgc tcgtccgcgg tgaaaccgcc cgccgtcgtg
      961 gcctcgagga cgtgggggta cgcggccggg tcgacgacga cggcgaccga accgtggttc
     1021 ttggcggcgg cgcgcaccat cgagggcccg ccgatgtcga tcttctcgat gcactcggcc
     1081 acgctcgccc ccgacgcgac ggtgtccacg aaggggtaga ggttcaccac gacgaggtcg
     1141 aacgcctcga cgccgagggc ctccagctcg gccacgtgcc ccgggttggt gcggtccgcg
     1201 aggatgccgg cgtggattcg gggatggagg gtcttcacgc ggccgtcgag gcactccggg
     1261 aagcccgtga gggtcgccac ctccgtgacg ggcacccccg cggccgcgat cgtggcggcg
     1321 gtggacccgg tggagacaat ggtgacgccg gcggcgtgca gcccgcgggc aagggcctcg
     1381 aggcctgtct tgtcgaatac ggagacgagc gcgcggcgga tgggcgtgta agtcatcggg
     1441 aggctcctcg tgaggtggca cgggcaccca ggcgtgcgat gtccgatccg atggtccggc
     1501 cgctccccgg tggtactcca ccttcgccag tcgcgaccgc ccccagtcta cggcgtcgcg
     1561 cggcccccga atgagacgcg gcgtccgtcg acgcgccaac cctcacgggc cagccggccc
     1621 acggactcca cgagctgtgc ccgctcggcc accttgatgc gctcggtgag cgtctcctcg
     1681 tcgtcgtccg cgaggacggg cacggccacc tgcgcgagga tcggaccggt gtccacgccg
     1741 gcgtcgacga ggaagagcgt ggcacccgtg agcttcaccc cgtacgcgag ggcatcccgt
     1801 ggcccgtgga tccccgggaa ggacggcagc agggcgttgt gggtgttgat gtaccggtcc
     1861 ccgaaccgct cgaggaacac cggaccgcag agcttcagga acccggcgga gaccaccagg
     1921 tccgggcgga gggcggcgac ggcgtcggtg agcgccgcgt cccagtcggc gcgcgtgggg
     1981 aggtcccgga ccgccagttc gaaggtgggg atgcccgccg cgcgcgcgat gcccactccc
     2041 ccggtgcccg gccggtccgc gcccacgccc acgatggtgg caccccacgc ggggtcgcgg
     2101 gaggcgtcca ggagcgcggc gaggttggaa cccccgccgg agacgagcac gacgacgcgg
     2161 aactgctgtt gcaccacacc agcctagcgg cgcgggtcct cgaccaggag cgtgcgtccg
     2221 gggcccgccc cggccgcgga ggcggccgtc ggggaggtca cggccgtcgc ggccgtcggg
     2281 tcgatcgccg cggtcgggtc ggtggcggtc gggtcggtgg cgggcgcggg agcggcgtcc
     2341 gccaggggga gggcgtcgtc gtcgggaacg ctcgtgacgg tgtcgtccac
//
LOCUS       DASRKH010000118         2077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_14980, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000118 DASRKH010000000
VERSION     DASRKH010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14980"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="VFC06_02010"
     CDS             complement(<1..791)
                     /locus_tag="VFC06_02010"
                     /inference="COORDINATES: protein motif:HMM:NF037721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HZL80706.1"
                     /translation="MRRRVLGATLSALAVAMVLLGIPLGVFAFQLVRDDALRDLDSRT
                     AAAARSIEARYDSGETIDESILDNFLGTASGAPRHIAVRLPDGTEITAGEEPQPSFRG
                     SAVTDSDIIVLVSIARADVFWEAARAVGLVLVVGGVAVLAGAVMASWQANRLAAPLVY
                     LAASAEQIGSGQTRPLIELSGVEEIDLVAEELVRSADRMAARLSAERQFAADASHQLR
                     TPLTALTMRLEEISASTTQDEVRVEAEASLEQVDRLVGVVDELLGR"
     gene            complement(795..1469)
                     /locus_tag="VFC06_02015"
     CDS             complement(795..1469)
                     /locus_tag="VFC06_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009055079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZL80707.1"
                     /translation="MARIVLVEDDPTISEPLTRALGREGYEVEWHGTGQGGVGAAHSA
                     DLVILDLGLPDIDGVDVARRIREQGLSVPILMLTARADEVDLVVGLDAGADDYVTKPF
                     RLAELLARVRALLRRNADAELAGEVSAGGVRVDVSGHRAYLDDKELQLSGKEFDLLQV
                     LAAHAGSVVSRDQLMREVWDADPHAPSKTLDMHVSWLRRKLGDDAAKPRFITTVRGMG
                     FRFEND"
     gene            1552..>2077
                     /locus_tag="VFC06_02020"
     CDS             1552..>2077
                     /locus_tag="VFC06_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80708.1"
                     /translation="MKTAPDALRTRIRSVVDPLGRVGWSGTPEFVVVVLVALALAAAT
                     TASLGIHHPYTTLPLAALFTWGSWLAVRPHATQDSPGARLASQWALLGVVLWIVVGIA
                     LSGEYLIVTRDPGFLTLTGVWLTDHASSDIPTLGALQVADAQQNVIADAWQAWNLRGD
                     VVQPQGAKALPALIS"
BASE COUNT          357 a          741 c          654 g          325 t
ORIGIN      
        1 cgccccagca gctcgtccac cactcccacg aggcgatcga cctgttcgag agaggcctcc
       61 gcctctaccc gcacctcgtc ctgcgtcgtg gaggccgaga tctcctccag ccgcatcgtc
      121 agggccgtga gcggcgtccg caattggtgt gacgcgtcgg cggcaaactg gcgctcggca
      181 gacagacgcg ccgccatccg gtccgcactg cgcaccagtt cctcggcgac gaggtcgatc
      241 tcctccacgc cgctcaattc gatgagcggc cgggtctgac ccgaaccgat ctgctccgcg
      301 gacgcggcca agtacaccaa gggcgcggct aagcgattgg cctgccacga tgccatgacg
      361 gcgcccgcga gaacagcgac gcctccgacg acgagcacca agccgaccgc cctagcggcc
      421 tcccagaaca cgtcggcccg agcgatcgag accaacacga tgatgtcgga atccgtgacg
      481 gcgctcccgc ggaacgatgg ctggggttcc tctcccgccg tgatctcggt cccgtccggc
      541 aggcgcacgg caatgtggcg tggcgccccg gacgccgtgc cgaggaagtt atcaagaatg
      601 gactcgtcaa tcgtctcgcc cgagtcgtag cgcgcctcga tggaacgggc ggccgcggca
      661 gtccgagaat ccaggtctcg cagggcatcg tccctgacga gttggaaggc gaataccccc
      721 aaggggatgc ccagcagcac catggccacc gccaaggcgg agagcgtggc ccctaggacg
      781 cggcggcgca ccgctcagtc gttctcgaaa cggaagccca tgccccgcac cgtggtgatg
      841 aagcgcggct tcgcggcatc gtcgcccagc ttgcggcgga gccaggacac atgcatgtcg
      901 agagtcttcg acggggcgtg agggtcggcg tcccacacct cgcgcatgag ctgatcgcgc
      961 gacacgaccg aaccggcatg cgcggcgagc acctgaagaa ggtcgaactc cttgccgctg
     1021 agctgaagtt ccttgtcgtc gagatacgcg cggtgtcctg agacgtccac cctgacgcct
     1081 cccgcagaga cctctcccgc cagctcagca tcggcgttgc gtcgcagcaa cgcgcgcacc
     1141 cgggcaagca gttcggcgag gcgaaacggc ttcgtgacgt agtcatcggc gccggcgtcg
     1201 aggccgacca ccaggtctac ctcgtccgcg cgcgcagtca gcatcaggat cgggacggag
     1261 agtccctgtt ccctgatgcg cctggcgaca tccacgccgt cgatgtcggg aagccccaag
     1321 tcgagaatca ccaggtcggc cgaatgagcg gcccccaccc caccttgacc ggtgccgtgc
     1381 cactccactt cataaccctc gcgccccaaa gcccgggtca agggttctga aatggtgggg
     1441 tcatcttcga ccaagactat tctcgccatg gggcaacatt acccgcgccg gccgactttg
     1501 caatgccggg cggcgccgca cgcaacacct tcggattgat agtgtccgga cgtgaagact
     1561 gcgcccgacg ccctgcgtac gcgaatacgg tctgttgtcg accccttggg ccgcgtggga
     1621 tggagcggaa cccccgagtt tgtcgtggtg gtgctggtcg cgctcgccct ggccgccgcc
     1681 acgacagcct cgctgggaat ccaccacccg tacacgaccc tcccgctcgc agcgctgttc
     1741 acctggggat cttggctggc ggtcaggcca cacgccacgc aagactcacc cggagcacgc
     1801 ctggccagcc agtgggcgct cctcggcgtg gtgctgtgga tcgtcgtcgg catcgcgctc
     1861 tcgggcgagt acctgatcgt cacgcgtgac cctggcttcc tcactctcac tggggtgtgg
     1921 ctcaccgacc acgcgtcgtc ggacatcccc acccttggcg ccctgcaggt cgccgatgct
     1981 cagcagaatg tgatcgccga cgcttggcag gcctggaacc tgcgcggcga cgtcgtgcaa
     2041 ccacagggag cgaaggcctt gccggccctc atctcgg
//
LOCUS       DASRKH010000119         2140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_37236, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000119 DASRKH010000000
VERSION     DASRKH010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37236"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VFC06_02025"
     CDS             complement(<1..233)
                     /locus_tag="VFC06_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80709.1"
                     /translation="MSTAAAEAKRKAQAEARAQARRRAVGWTVAGVVVVAMFAALVAY
                     ILREGAIDDVVVAGPSGAPVISSDGGLGVGSSGV"
     gene            complement(325..1128)
                     /locus_tag="VFC06_02030"
     CDS             complement(325..1128)
                     /locus_tag="VFC06_02030"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HZL80710.1"
                     /translation="MAKKDLGSDRVALAKKQAQAQVRAQERRTAVIWIVIGVILVALF
                     AALVAYIVRQSDVAAVGNGEQSTPAVASENYGFPVGNTGAVGEGLDDSRVRVDIYLDF
                     MCPICGFFEETQGPTLDSLRADGTADVYYHPISILDRASQNTEFSTRSASAAALVAQE
                     SPENFLAFLKAMFVNQPAENTTGLTDAEIQAIAAAAGVPDDVVAKIPDHAYSSWVRSA
                     TEQASVDGLKGTPTVAINGVMQDAQANPDDLNWSTEGALLKFVQDAAGK"
     gene            complement(1139..1672)
                     /locus_tag="VFC06_02035"
     CDS             complement(1139..1672)
                     /locus_tag="VFC06_02035"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HZL80711.1"
                     /translation="MSQTAAAEGTSAKARWRLVQPWISLLVRLAMGIIMIAAAVPKMM
                     DVDESVRAVRAYRLIPEAFVPFVGTALPYFEFVFGLVLIVGVLVRWSAILWLALMAVF
                     VFGVAWAWAKGYSIDCGCFGGGGDVAEGTTDYPGHMLERAGFVALGVFLFVFPRTAIS
                     LDAWMRGESLVATSRPE"
     gene            complement(1749..>2140)
                     /locus_tag="VFC06_02040"
     CDS             complement(1749..>2140)
                     /locus_tag="VFC06_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80712.1"
                     /translation="PWHIVRALVELLPSLLAAAAIGIGVATLGWWLVSSGAAAPVAAD
                     TQVWGHAMALAVGALAFIGALWWGLWSWRTREGAYRVTEALAPTTGVSAAWIVVALIV
                     VASFVVAVYLGADPWWWPFPQMPQAAG"
BASE COUNT          410 a          759 c          640 g          331 t
ORIGIN      
        1 accccgctgc ttcccactcc gagcccgccg tcgctgctga tcacaggagc tccggagggc
       61 cccgccacca ccacgtcgtc aatagcgccc tcacgcaaga tgtacgccac gagcgcggcg
      121 aacatggcaa cgaccacgac gcccgcgacg gtccagccca ccgcacgccg ccgagcctgg
      181 gcgcgcgcct cagcctgcgc cttgcgcttc gcctcggctg ccgctgtgga caatcccgcg
      241 gacttcgacc cgatgaccta cccccttcga tctcggcttc tgccgggatc gacgcggaaa
      301 ctcgaccggc cgaaaggcga gtcgctactt ccctgcggcg tcttgcacaa acttcagcaa
      361 cgcaccctca gtggaccagt taaggtcatc aggattggcc tgagcatcct gcatgacgcc
      421 gttgatcgcg acggtgggtg tgcccttcaa tccgtcgaca ctcgcctgct cggtggcgct
      481 acgcacccac gacgagtagg cgtggtccgg gatcttcgcg accacgtcgt ctggcactcc
      541 cgccgccgcg gcgatcgctt ggatctccgc atcggtcagg cccgtggtgt tctctgcggg
      601 ctggttgacg aacatcgcct tcaagaacgc caggaagttt tcgggcgact cctgcgcgac
      661 gagcgccgca gccgatgcag aacgcgtcga gaattcggtg ttctgtgaag cacggtcgag
      721 gatcgagatc gggtggtagt acacgtccgc cgtgccgtcc gcacgcaacg agtcaagcgt
      781 cggtccttga gtctcctcga agaatccgca aatggggcac atgaagtcga ggtagatgtc
      841 cacccgcacc cggctgtcgt cgagaccctc gccgacagcc cccgtgttgc ccaccgggaa
      901 gccatagttc tcagacgcca cggcgggcgt ggactgttcg ccgttcccga cagcggccac
      961 atcgctttgg cgcacgatgt aggcgactag cgccgcaaac agggccacca gaatgacgcc
     1021 gatcacaatc cagatcaccg cagtgcggcg ctcttgggcg cgcacctgtg cctgggcttg
     1081 cttcttggcc aatgcgaccc tgtcagaacc taggtccttc tttgccatgg gggactcctt
     1141 attcgggtcg gctcgtggcg accaaggact cgccgcgcat ccatgcgtcg agcgagattg
     1201 ctgtacgggg gaacacaaac aggaaaacgc caagcgccac aaatcctgca cgctcaagca
     1261 tgtgcccagg atagtcggtg gtgccctcgg cgacgtcgcc cccgccgcca aagcagccac
     1321 agtcgatgct gtagcccttt gcccacgccc aggccactcc aaacacgaac accgccatca
     1381 aggccagcca cagaatcgcc gaccagcgca cgagcactcc aacgatgagg acgagcccaa
     1441 acacgaactc aaagtacgga agtgccgtgc cgacgaacgg gacgaaggcc tcgggtatca
     1501 ggcgatacgc gcgcaccgcg cgcaccgact cgtcgacgtc catcatcttg gggaccgcgg
     1561 cggcgatcat gatgattccc atcgcgaggc gaacaagcag actgatccat ggctggacca
     1621 ggcgccaacg cgccttcgcc gaggtgccct cagcggccgc cgtctgtgac atgacgttct
     1681 cccttgctcg tccggtgaac gcattctagg tcgctcaagt tccctggtgg cggggccgga
     1741 gtcccgacct aaccggcggc ctggggcatt tgcgggaatg gccaccacca agggtcggca
     1801 cccaggtaga ccgccaccac gaacgaagcg acgacgatga gcgccaccac gatccacgcg
     1861 gcgctcactc ccgtcgtcgg agcgagtgct tcggtcacgc gatacgcccc ctcgcgcgtg
     1921 cgccatgacc acagtcccca ccacagcgcg ccgatgaaag ccagtgcccc cacggcaaga
     1981 gccatggcgt gcccccacac ctgcgtgtcg gccgcgacgg gcgcggcggc gccgctagaa
     2041 accaaccacc agccgagcgt ggctacgccg attccgatcg ccgctgcggc gagcagcgaa
     2101 ggcaagagtt cgacgagggc tcgcacgatg tgccacggga
//
LOCUS       DASRKH010000120         3050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_63856, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000120 DASRKH010000000
VERSION     DASRKH010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63856"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="VFC06_02045"
     CDS             complement(<1..731)
                     /locus_tag="VFC06_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80713.1"
                     /translation="MSGSLPVTDHGDVEPEVRASAQDSWAPSRPSRPPVTGSFPAVRP
                     DDLTPTKPQMPLSAHSAVARQIPPAAPEHTAAPEPEHTAAPEPEQTAEPEQVAEPEPT
                     ADSIPTPIEADEDEIIAPILPPPDTEIMPRAGGDESKVGDAPASGTTPWSPTESSEWP
                     SEREFSPAPTDSVRQLGASAISNASLQAAGAPTPISGVPRVPEHENEFPSFGDEEPFV
                     PRFEPSASTTPMPTPTPTVSFAEPAP"
     gene            1530..2909
                     /gene="radA"
                     /locus_tag="VFC06_02050"
     CDS             1530..2909
                     /gene="radA"
                     /locus_tag="VFC06_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HZL80714.1"
                     /translation="MATPKKTAPSYRCTECGWTAVKWVGRCGECQQWGTVVEVGVAAG
                     PRTKASAIATPARPIGDVPSSESARHPTRVAEFDRVLGGGLVAGAAVLLAGEPGVGKS
                     TLLLEVAARAAREGTDVLYISGEESAGQVRLRAERMGALEPRLKLAAETDLGVVLAHL
                     AVEAAGLVIIDSIQTIASASIEGSAGGVSQVREVASALVQAAKISGVPMVLVGHVTKD
                     GSVAGPRLIEHLVDVVCQFEGDAHSPLRLLRALKNRFGSVDEVGCFQLVEAGIEEVPD
                     PSGLFLSQTGEPIAGTCATITIDGRRALPAEIQALVAPSALSNPRRATSGMDSARVAM
                     MLAVLHRRVGVALVADDVYVSTVGGAKVAEPAADLALALALASARADRPVRKGFIAVG
                     EVALSGAVRPVSAIGTRLSEAARLGFTHAIVPVGVDASAREAVAGRMTVIAVETLADA
                     VRAGVTPAQ"
BASE COUNT          415 a          942 c         1174 g          519 t
ORIGIN      
        1 ggagcgggct ccgcgaaact cacggtcggc gtcggcgtcg gcatcggcgt tgtgctcgcc
       61 gacggctcga agcgcggcac gaacggttcc tcgtctccga acgacgggaa ctcattttcg
      121 tgctcaggaa cgcggggaac gcccgatatg ggcgtggggg cgccagcggc ttgcaggctt
      181 gcattcgaga tcgcggaggc gccgagttgc ctcactgaat ctgtaggtgc cggggagaac
      241 tctctctcgc ttggccactc agaggattcg gtcgggctcc aaggcgtggt ccccgacgcc
      301 ggtgcgtcgc ccaccttgct ctcgtcacct cccgcgcgcg gcatgatttc cgtgtccggc
      361 ggaggcagga tcggggcgat gatttcatcc tcgtctgcct caatcggggt ggggatcgag
      421 tccgcagtcg gctcgggctc tgcaacttgc tcgggctctg cagtctgctc aggctccggt
      481 gcggcagtgt gctcaggctc cggtgcggca gtgtgctcag gcgcggctgg gggaatctgt
      541 cgcgcaaccg cagaatgggc gctgagcggc atctgcggtt tggtgggcgt gaggtcgtcg
      601 ggacgcacgg cggggaagga cccggtcacg ggtgggcgcg acggacggct tggcgcccac
      661 gagtcctgtg cgctcgcacg cacctccggc tcgacgtcgc cgtgatcggt gacggggagg
      721 gacccgctca agtacgggct cgtgcgaatg acagagggcg gcggcgtcgt cggctctgcc
      781 agcggagggt cttcgggcgc gggcggcgca ggctcgaact tttgccaagg ctcgaacgcc
      841 ggcggaggca cgaaggctgg ggcgggcttg aattctgccg gaggctcgga cgctcgcggc
      901 ggcgtccgga ccgccgctga ctcgggttcg tcgctggtcg gctcgggctc gactgccgcg
      961 gtttcgcgcc ggaccccggc gcggtcgcca cgcacaccga agaaggcagc cgcagcggcg
     1021 ggcgacgggc tagatcccgt gggcgggact gccgcggagg gatcggcgaa cgaagcatcg
     1081 gtggctgtcg gagtcggagt cagagtcgga gtcagagtcg gagtcggagt cggagtcggc
     1141 gtcagcgaag gcgcaggcgc aggcgtgggc gcaggcgcag gcgcagcggt gaacggccaa
     1201 gcggtgagcg gcgcgctggg aagcgcggac tcaggtgcag ctgcaggtgc cggtgcgggt
     1261 gcagcctcag acgcgatcgg cgttggcggg tcctcgcgct cggctacggc ttcaggggcc
     1321 ggctccgcct cggattctgc gggtaccact gggtcgggct ccgcctcggg agcgttgtcg
     1381 ctggtctcag cgggctccgg cacgggattc accggcagcc acgggggacg gtactcgtca
     1441 ctagccatgg gcccaggtta tgcccagggg ggtgcacgcg gctgttcgtg cggcccgcgc
     1501 gtgtcagatc gggcaggtag cgtgacgcca tggccactcc caagaagacc gcgccctcct
     1561 accggtgcac cgaatgcggg tggaccgcag tcaagtgggt cggccgctgc ggcgagtgcc
     1621 aacagtgggg caccgtggta gaggtgggtg tcgcagccgg gccacgtacc aaggcctccg
     1681 ccatcgccac accggctcgg cccatcggcg acgttccttc cagtgagtcg gcgcgccacc
     1741 ccacgagggt ggctgaattc gatcgcgtcc tcggcggcgg gctggtggcc ggtgcggcag
     1801 tgttgcttgc aggggagccg ggcgtgggca agtcgacgct gcttctcgag gtggcggcac
     1861 gggcggctcg tgagggcacg gacgtcctct acatctctgg cgaggaatcc gcggggcagg
     1921 tgcggttgcg tgccgagcgc atgggggcgc ttgagccgcg actcaagctg gccgccgaaa
     1981 ctgacttggg cgtcgttctc gcgcacctgg cggtggaggc cgccggtttg gtgatcatcg
     2041 actcgatcca gaccatcgcg tcggcgtcga tcgagggatc tgcgggaggt gtgtcccaag
     2101 tgcgcgaggt ggcatccgcg ctggttcagg cggcaaagat ctcgggggtc cccatggtcc
     2161 tcgtgggtca cgtcaccaag gatggctccg tggcgggtcc gcgactcatt gagcacctgg
     2221 tggacgtggt gtgccagttc gagggcgacg cgcactcacc attgaggctg ctgcgtgccc
     2281 tcaagaatcg ttttggatcc gtggatgagg tgggctgctt ccagttggtg gaggcgggca
     2341 tcgaggaggt ccccgatccg tccggcctgt tcctctctca gaccggagag cccatagccg
     2401 gaacgtgcgc gaccatcacg atcgacggtc gacgtgcgct ccccgcggag atccaggcgc
     2461 tcgtggctcc ctccgcgttg tcgaatccgc gtcgggcgac gtccgggatg gattccgcgc
     2521 gcgtggccat gatgctcgcg gtgctgcacc ggagggtggg cgtcgcgctc gtcgcggacg
     2581 acgtctacgt ctcgaccgtg ggcggagcca aggtggctga accggcggcg gacctcgcgc
     2641 tggccctggc tctcgcatcc gcacgtgcgg accggcctgt gcgcaaggga ttcatcgccg
     2701 tcggggaagt ggcgctgtct ggcgcggtgc gccccgtgag cgccatcggc acccgcctgt
     2761 ctgaggccgc caggctgggc ttcacccacg cgatcgttcc ggtgggagtc gacgcatcgg
     2821 cgcgcgaggc cgtcgcgggg cgcatgacgg tgatcgccgt ggaaaccctc gccgacgccg
     2881 tgcgagctgg cgtgacgccg gcgcagtaac gggctagctc cgcagtgttg ctaactgccc
     2941 agtgttgcta actgcccagt gtggctagct ccgcagtgtg gctaactgcc cagtgtgaag
     3001 gtgacgtcgg gtgcttcgat cccctggatc gtgagcacgg cgtgataggt
//
LOCUS       DASRKH010000121         2107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_36579, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000121 DASRKH010000000
VERSION     DASRKH010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36579"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..1005)
                     /locus_tag="VFC06_02055"
     CDS             complement(145..1005)
                     /locus_tag="VFC06_02055"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HZL80715.1"
                     /translation="MSIVAPAVKTPAARTPSSGRDLPVAIVVGVTLIAVVIASLFFRK
                     EAFVLVAVLAVGAGLWELAQAFGRRGIHLPLLPLLVGDVGIIVSAYTAGAEALFVAFL
                     LTVGGVVVWRVLDGNGEPALRDSTAGTFAAAYLPFLAGFVMLLLAMPDGASRVLLFIL
                     LCVASDTGGYAVGSLIGRHPLAPSVSPHKTWEGLLGSVVLACFVGVVGLQLLFHGDPM
                     VGVFLGVATVFTASIGDLAESLLKRDLELKDMGSLLPGHGGVLDRIDSMLLTAPAVYL
                     ILNVLQPVVS"
     gene            complement(1005..1562)
                     /gene="frr"
                     /locus_tag="VFC06_02060"
     CDS             complement(1005..1562)
                     /gene="frr"
                     /locus_tag="VFC06_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HZL80716.1"
                     /translation="MIDETLLEAEEKMDKAVEVAKEDFATIRTGRANAAMFSRIFVDY
                     YGTPTPLQQLASFNVTEARTILIAPFDKSSMTAVEKALRDSDLGVNPSNDGNVIRVML
                     PALTEERRKDFVKLARAKAEDARISVRNIRRRAKDELDRMVKDGASGEDEVARAEKEL
                     EALTKKHVDVIDTVLASKENELLEV"
     gene            complement(1616..>2107)
                     /locus_tag="VFC06_02065"
     CDS             complement(1616..>2107)
                     /locus_tag="VFC06_02065"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0044210 - 'de novo' CTP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine monophosphate kinase"
                     /protein_id="HZL80717.1"
                     /translation="MLGTVMNCLALQDFLEQAGVVTRVQTAITMGQVAEPYIPLRAIR
                     HLEKGRVVIFGAGAGMPYFSTDTVSVQRALETHCQEVLMGKNGVDAVYSADPRTDPNA
                     VRLEHLTFTDALVNGLEVMDATALSLCRDNDVRMRVFGMTEPGNVTRALQGEKIGTLV
                     TAN"
BASE COUNT          335 a          719 c          723 g          330 t
ORIGIN      
        1 ccctctgctc gggcgtcagg tcgacgaagt ggcgcggcgg cttgccccgt gcgcctcgcg
       61 tcggcgcggc aagattcagc gtgacgggtg tcccgctcat gccgggcacc ccctctcggt
      121 cgatagctct gggacgtgca cgggtcagct cacgaccggc tgcagcacgt tgaggatcag
      181 gtagacggcc ggcgccgtca ggagcatcga gtcgatgcgg tccagcactc cgccgtggcc
      241 cggcaggagc gagcccatgt ccttcagctc gaggtcgcgc ttgagcaggg actccgcgag
      301 gtcgccgatc gacgccgtga agactgtggc cacgcccagg aagacgccca ccatcgggtc
      361 gccgtggaac agcagctgca gcccgacgac gccgacgaag caggcgagca cgaccgagcc
      421 gagcagaccc tcccaggtct tgtgcgggct caccgagggt gcgagcgggt ggcggccgat
      481 gaggctcccg actgcgtacc ccccggtgtc gctcgcgacg cacaagagga tgaagagcag
      541 cacacgtgat gctccgtcgg gcatcgcgag caggagcatg acgaagcccg caaggaacgg
      601 caggtacgcc gccgcgaagg tccccgcggt cgagtcgcgc agggcaggtt cgccgttgcc
      661 gtcgagaacc cgccacacga cgacgccgcc gacggtgagg aggaaggcga cgaagagcgc
      721 ctctgcgccc gccgtgtagg ccgagacgat gatgccgacg tctcccacca ggagcgggag
      781 cagcgggagg tggatccccc gacgaccgaa cgcctgcgcg agctcccaga gacccgcacc
      841 cacagcaagg accgcgacga ggacgaaggc ctccttgcgg aagaacaacg acgcgatcac
      901 gacggcgatc agcgtcactc cgacgacgat ggcgacgggc aggtctcgcc cactgctcgg
      961 ggtgcgggcg gcgggcgtct tcacggcggg tgcgacgatc gacatcagac ctcgagcagc
     1021 tcgttctcct tggaggcgag cacggtgtcg atcacgtcga cgtgcttctt cgtgagggcc
     1081 tcgagctcct tctcggcgcg cgcgacctcg tcctcacccg atgcgccgtc cttcaccatg
     1141 cggtcgagct cgtccttcgc gcgccgacgg atgttgcgga ccgagatcct cgcgtcctcg
     1201 gccttcgccc gagcaagctt gacgaagtcc ttgcggcgct cctcggtgag ggcaggcagc
     1261 atcacgcgga tgacgttgcc gtcgttcgac gggttgacgc cgaggtccga gtcgcgcagg
     1321 gccttctcga cggcagtcat cgaggacttg tcgaacggcg cgatgaggat cgtgcgggcc
     1381 tcggtcacgt tgaacgacgc cagctgctgc agcggcgtcg gtgtgccgta gtagtcgacg
     1441 aagatcctgg agaacatcgc ggcgttcgcg cgtcccgtcc ggatcgtcgc gaagtcctcc
     1501 ttcgcgacct cgaccgcctt gtccatcttc tcctcggcct cgaggagggt ctcgtcgatc
     1561 accggtgctc cttcatgtgc gtcacgtgcg tgggctgtgt ccgggcgaac ctgggtcagt
     1621 tcgcggtgac cagcgtgccg atcttctcac cctgcagcgc gcgcgtgacg ttgccgggct
     1681 cggtcatccc gaacacgcgc atccgcacgt cgttgtcccg gcacaggctc agcgcggtcg
     1741 cgtccatgac ctcgagcccg ttgacgagcg cgtcggtgaa cgtcaagtgc tcgagccgga
     1801 cggcgttcgg gtcggtgcgc gggtccgcgc tgtagaccgc gtcgaccccg ttcttgccca
     1861 tgagcacctc ctggcagtgc gtctccaggg cgcgctgcac cgagacggtg tccgtcgaga
     1921 agtacggcat ccccgcgccc gcgccgaaga tcacgacgcg acccttctcg aggtggcgga
     1981 tcgcgcggag cgggatgtac ggctcggcca cctggcccat cgtgatcgcg gtctgcacgc
     2041 gtgtcaccac gccggcctgc tcgaggaagt cctgcagtgc caggcagttc atgaccgtgc
     2101 ccagcat
//
LOCUS       DASRKH010000122         2172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_14127, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000122 DASRKH010000000
VERSION     DASRKH010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14127"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..160)
                     /locus_tag="VFC06_02070"
     CDS             complement(14..160)
                     /locus_tag="VFC06_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80718.1"
                     /translation="MGILAGAVVLLAVPAALVPWHPKGSLRAIVSFLAAATVVGIAWA
                     IMNP"
     gene            430..999
                     /locus_tag="VFC06_02075"
     CDS             430..999
                     /locus_tag="VFC06_02075"
                     /inference="COORDINATES: protein motif:HMM:NF024734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HZL80719.1"
                     /translation="MAVTTKLERLREVALDQHGLVTTAQAAEEGVSNAELSMMVARDR
                     LERVAHGVYRVPQVPQTEFDQYQLAVLWTGVPEACLSHDTALAAWNISDINPDRIHVT
                     VSRHRRIRRAGGERYEIHYEDVDAHGVTWWQGIRTVDVPTAIGQAISSGVPTYLIRQA
                     LDRAGRTSRLPAKDRERLANELEARDGGA"
     gene            986..1933
                     /locus_tag="VFC06_02080"
     CDS             986..1933
                     /locus_tag="VFC06_02080"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HZL80720.1"
                     /translation="MVEPEPSRLTQALDGLSPKAKPPGSARILNAWITQAQDALGVAG
                     SRVGWLVAATVVSAALQRAVDAKGSPLFLLKGGTMLQYRLPGISRATQDVDGLIRGDL
                     DQFLATLDDVLGEPWGPLTLVRGEVETIDVPHRLVKPRRFHITVLANGVTWRRIQVEI
                     APDEGGAGAEPDRIASPSLAGFGLPTPDGLASLSMRYQIAQKVHACTDPHDPPAHVND
                     RPRDVVDLLLLRDLVAATGSPTLDEIRYAIEDVFASRAREAKATGGSSRAWPTTVTAH
                     QHWGPSFGRAAESAGLSISMSDAVAQVNDWLALVEQARA"
BASE COUNT          377 a          702 c          727 g          366 t
ORIGIN      
        1 ccgcgacgat gaatcagggg ttcatgattg cccacgcgat tcctacgacc gtggctgcgg
       61 cgagaaacga gacgatggcg cgcaacgatc ctttggggtg ccacggcaca agcgcggcgg
      121 gcacggcgag caacacgacc gcaccggcga gtatgcccac ggcaagcccg atgtcgggat
      181 ggcgcccaaa aacgctgggc cgatgctgct gaggttcaga cacgtcactc aagtgcaacc
      241 cgcgatccgc atgcgccaag tgggttcccc catggacgtg gagcgtactc cgctacccgt
      301 cagggagggc ttcggtccaa gccgtgcggt gcgcctatct tccttgaata acgcatagtc
      361 atattgaggg atcgagtcca ttgacaaggc gctcgtgcca aaaccgtcac acggtgttaa
      421 gattttgaca tggccgtgac cactaagctc gagcgtttgc gcgaggtcgc gctcgatcag
      481 cacggcctcg tcaccacggc ccaggccgcc gaagagggtg tgtcgaacgc cgaactgtcc
      541 atgatggtgg cccgcgaccg gctcgaacga gtggcgcatg gcgtctaccg ggtccctcag
      601 gttccccaga cggagttcga tcaataccag ctcgccgtgc tgtggaccgg agtcccggag
      661 gcgtgcctca gccacgacac cgcgctcgcc gcatggaaca tcagcgacat caatcccgat
      721 cgcatccacg tcacagtgtc gcgccatcga cgcatccgca gggctggcgg cgagcgctac
      781 gagatccact acgaggatgt cgatgcacat ggcgtcacct ggtggcaggg gatccgcacg
      841 gtggacgtgc ccacggcgat cggccaggcc atttcgtcgg gcgttccgac gtacctcatt
      901 cgtcaggcgt tggaccgcgc gggccgcacc agccgtctgc cggccaagga tcgcgagcga
      961 ctagcaaacg agttggaggc tcgcgatggt ggagcctgaa ccgagcaggc tgacgcaggc
     1021 actcgacggc ctgagcccca aggccaagcc gccaggctcg gcgcgcattc tcaacgcgtg
     1081 gattactcag gctcaggacg cgctgggcgt ggcggggtcg cgagtcggtt ggctggtggc
     1141 cgcgaccgtc gtgtctgcag cgctccaacg cgccgtcgac gcgaagggct cacccctgtt
     1201 cttactcaag ggcggcacga tgcttcagta ccgcctgccg ggcatctccc gtgccactca
     1261 agacgtcgat ggtttgatcc gtggggacct cgaccagttc ctcgcgacct tggacgacgt
     1321 actgggtgaa ccgtggggtc ccctgacgct cgtgcgcggc gaggtggaga ccatcgacgt
     1381 cccgcatcgg ctggtgaagc cgcgccggtt ccatatcacc gtcctcgcga atggtgtgac
     1441 gtggcggcgc atccaggtgg agatcgctcc cgacgaaggt ggagcgggcg cggagcctga
     1501 ccggatagcc tccccatcgc tcgctgggtt tgggctgccc acgcccgacg gcctggcgag
     1561 cctgtccatg cgttaccaga tcgcgcagaa ggtccacgcg tgcaccgatc cacacgatcc
     1621 gcctgcgcat gtcaacgacc ggccgcgcga cgtggtggat ctgttgttgt tgcgcgacct
     1681 cgtggccgcc accggatcgc cgacgctcga tgagatccgg tatgccattg aggacgtgtt
     1741 cgctagccgt gcgcgcgagg ccaaggcgac cggaggctcg tcgagggctt ggcccacgac
     1801 tgtcactgca caccagcact gggggcccag cttcggcagg gcggcggaat ccgctggcct
     1861 atcgatctca atgtccgacg ccgtggccca ggtcaacgac tggcttgccc tcgtggagca
     1921 agcgagggcg taggcggcga tcgaggtgcg cacaatctct aggcacggct ccaggccgct
     1981 gggggaggac ccggtctcgg cgatcggcac agtggaaatc taggtccctg ccgccgcgat
     2041 ctgctggacg aaggcgttgg tccaggcgac ggcttcttcg acggttggca acacgtcgag
     2101 gccttcggcg gcttccgcgt acagcgtgtc cctctgctga ccgacgacga cggtgggtgg
     2161 ccaggcctgt tg
//
LOCUS       DASRKH010000123         2232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_50784, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000123 DASRKH010000000
VERSION     DASRKH010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50784"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..>2232
                     /locus_tag="VFC06_02085"
     CDS             182..>2232
                     /locus_tag="VFC06_02085"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019812 - type I site-specific
                     deoxyribonuclease complex [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HZL80721.1"
                     /translation="MLRPNIILDLLANFTAYATDTKKRRIKIIARYQQYEGTNKIVER
                     VVAGHPKKGLIWHFQGSGKSLLMLFAARKLRLHPALKNPTVLIVVDRIDLDTQISSTF
                     YAADTPNLVKADSRAELARLLAQDVRKIIITTIFKFGEADGVLNDRANIIAMVDEAHR
                     TQEGDLGRKMRDVLPNAFLFGLTGTPINRADRNTFYAFGADEDTSGYLSRYGFEESIR
                     DGATMPLHFEPRLLELHIDKEAIDQTFKELTGELSDLDKDVLATTAAKMAVLVKSPER
                     IQTVCADIARHFQEKVAPSDFGAQVVTFDRESCLLYKRALDEYLPPEVSDVVMTVNSG
                     EDEYAPFRRERDAEEKLLDRFRDTADPLKMLIVTSKLLTGFDAPILQAMYLDKPLRDH
                     TLLQAICRTNRPYGDAKTHGLIVDYLGIFDDVAQAIQFDEEGITRVVTNITELVSKFP
                     AAMQSCLAYFAGVDRSVTGYEGLIAAQECLPNNDVRDAFAADYSYLSRLWEAISPDPV
                     LTQHESDFRWLTYVYESVKPSTGTGRLLWHVLGPKTIELIHENVHVDAIRDDLDTLVL
                     DADLLEAVLGAPDPNKKAKEIEIKVARRLRNRMHDPRFKALSERLEALKERHEQGLLL
                     SIDFLKALLELARDVVAAEQTAEPIDDEALGKAALTELFQEARNEETPIMVEKVVSDI
                     D"
BASE COUNT          454 a          759 c          647 g          372 t
ORIGIN      
        1 atcttgatgc ggcgcttctt cgtgtcggtg gcgtaggccg tgaagttggc aagcaggtcg
       61 agaatgatgt tcggtcggag catggaggcg acggcctcct cgatccggtg cagggccgtg
      121 gtgggtcgac gccgacgaaa agaccccggc cctgcaccgg atcgaggagg ccgtcgcctc
      181 catgctccga ccgaacatca ttcttgacct gcttgcgaac ttcaccgcct atgccaccga
      241 cacgaagaag cgccgcatca agatcatcgc ccgctaccag cagtacgagg gcaccaacaa
      301 gatcgtcgag cgcgtggtgg cagggcaccc gaagaagggc ctcatctggc acttccaggg
      361 ctcgggcaag tcgctcctca tgctcttcgc cgcccgcaag ctccggctgc atcccgccct
      421 caagaacccc accgtgctga tcgtggtaga ccgcatcgac ctcgacaccc agatctccag
      481 caccttctac gccgccgaca cccctaacct ggtgaaagcg gatagccgcg ccgagcttgc
      541 gcgactgctc gcccaggacg tgcgcaagat catcatcacc acgatcttca agttcggcga
      601 ggccgacgga gtcctcaacg accgcgccaa catcattgcc atggtcgacg aggctcaccg
      661 cacccaggaa ggcgacctcg gccgtaagat gcgcgatgtg ctgcccaacg ccttcctctt
      721 cggcctcacc ggcacgccca tcaaccgggc cgaccgcaac accttctacg ccttcggcgc
      781 cgacgaggac acgtccggct acctgagccg ctacggcttc gaggagtcca tccgcgacgg
      841 cgccaccatg ccactgcact tcgagccgcg cctgcttgag ctgcacatcg acaaggaagc
      901 catcgaccag accttcaagg aactcaccgg cgagctcagc gatctggata aggatgtgtt
      961 ggcgacgacc gccgccaaga tggcggtgtt ggtgaagtcc ccggagcgca tccagacggt
     1021 ctgtgccgac atcgcccggc acttccagga aaaagtggcg ccgagcgact tcggagccca
     1081 ggtggtcacc ttcgaccgcg agagctgcct gctctacaag agggcgctcg acgagtatct
     1141 gccgccggag gtctccgacg tggtcatgac ggtcaacagc ggggaggacg agtacgcccc
     1201 cttccgccgc gagcgcgacg ctgaggagaa gctcctcgac cgcttccgcg acaccgccga
     1261 tccgctcaag atgctgatcg tcacttcgaa gctcctcacc ggcttcgacg cgcccatcct
     1321 tcaggcgatg tatctggaca aaccgctccg cgatcacaca ctgctgcagg ccatctgccg
     1381 caccaaccgc ccctacggcg acgccaagac tcacggtctg atcgtcgact acctgggcat
     1441 cttcgacgat gtagcccaag ccatccagtt cgatgaggag ggcatcacgc gggtggtcac
     1501 caacatcacc gagttggtgt cgaagttccc tgcggcgatg cagtcctgtc ttgcctactt
     1561 cgccggcgtt gatcgcagcg tcaccggcta cgagggcctg attgccgccc aggagtgtct
     1621 accgaacaac gacgtgcgtg acgccttcgc tgccgactac agttacctca gtcgactctg
     1681 ggaggccatc tcgcctgacc ctgtgctgac gcagcacgag agcgacttcc gctggctgac
     1741 ttacgtctat gagtcagtga agccgtccac gggcacggga agactcctct ggcacgtgct
     1801 gggtcccaag accatcgaac tgatccacga gaatgtccac gtggacgcca tccgcgacga
     1861 cctcgatacc ctcgtgctcg acgccgatct gcttgaagcg gtactgggcg cacccgaccc
     1921 gaacaagaag gccaaggaga tcgagatcaa ggtagcccgt cgattgcgga accgcatgca
     1981 cgacccacgt tttaaggccc ttagcgagcg gctggaagct ctgaaggagc gtcacgagca
     2041 gggactgctc ctcagtatcg actttctgaa ggccctcctc gaactggccc gcgacgtcgt
     2101 cgctgcagaa cagaccgccg agcccatcga cgacgaggcg ctgggcaagg ccgcgctcac
     2161 cgaactcttc caggaggcca ggaatgaaga gaccccgatt atggtcgaga aggtcgtgag
     2221 cgacatcgac ga
//
LOCUS       DASRKH010000124         2665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_23316, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000124 DASRKH010000000
VERSION     DASRKH010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23316"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VFC06_02090"
     CDS             <1..209
                     /locus_tag="VFC06_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3052 family protein"
                     /protein_id="HZL80722.1"
                     /translation="TDALVDAQTVLDDGGVIWVLTPKAGRGGHIDHGEIEEAATTAGL
                     HATSTFSIAPDWSATRLGNRGRGK"
     gene            206..688
                     /locus_tag="VFC06_02095"
     CDS             206..688
                     /locus_tag="VFC06_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013601380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HZL80723.1"
                     /translation="MTVSPGSEQVVPELGKPAPDFASVDTHGTPVRLADLRGTPVVLV
                     FVPFAFSGTCTGELCAIRDSIELFDDAGVRLLVVSCDATYSLRAWQEQEGYTFDLLSD
                     FWPHGEIARTYSVFDDERGVAIRGSFLIDADGVLRWALVNPRTQARDLEAYRAALALL
                     "
     gene            726..799
                     /locus_tag="VFC06_02100"
     tRNA            726..799
                     /locus_tag="VFC06_02100"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:760..762,aa:Val,seq:tac)
     gene            844..1893
                     /locus_tag="VFC06_02105"
     CDS             844..1893
                     /locus_tag="VFC06_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HZL80724.1"
                     /translation="MRATVIHGPFDVRLETVPDPRIVLPTDAVVRVVAACVCGSDLWP
                     YRGVRPTKLPHRIGHEFVGVVEEVGADVRSVRPGQFVIAPFAISDNTCVHCRNGVHTS
                     CAQLGWWGEPDHDGIPSDAGQGEYVRVPLADGTLVATPDHPDAALLPSVLTLSDVMGT
                     GHHAALAGGVRPGSTVVVVGDGAVGLCAVLAARRLGAERIIAMSRHSTRQDLAVRFGA
                     TDIVAERGAEGVARVMDLLDGVGADAALECVGTKESMQQALDSARPGGRVGYVGVPAG
                     GPELPVQQMFSSNVGVLGGVAPVRAYISELMGDVWSGTIEPGLVFDLVVPLDDVAEAY
                     KAMDERRAIKSMLLP"
     gene            1916..>2665
                     /locus_tag="VFC06_02110"
     CDS             1916..>2665
                     /locus_tag="VFC06_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HZL80725.1"
                     /translation="MIDHPTAAPTLAEVVGVLDRLYPPGTAESWDAVGLVTGDPAQPV
                     RRVLYAVDPVDAVVDEAVAWGADLVVTHHPLLLRGVHSVAATTFKGALLHRLIRSGIG
                     LYAAHTNADAAPHGVAEALAAAVGLVDLEPLEPIAAEALDKHVVFVPVADAGRLVDAL
                     ALAGAGAIGAYTRCAWTATGEGTFTPSAEARPALGRAGEIATVVEARVEMVAPRRLRS
                     AVVAAMRAAHPYEEPAFDVLELARWDGPTGIG"
BASE COUNT          330 a          964 c          971 g          400 t
ORIGIN      
        1 tcaccgacgc cctcgttgac gcccagaccg tcctcgacga cggtggggtg atctgggtcc
       61 tcacaccgaa ggcaggtcgc ggcggtcaca tcgaccacgg cgagatcgag gaggccgcaa
      121 cgacggccgg gctgcacgcg acgagcacgt tctcgatcgc gccggactgg tcggcgaccc
      181 ggctcggcaa ccgcgggcgc ggcaagtgac ggtgtcgccc ggcagcgagc aggtcgttcc
      241 ggagctcggg aagccggcgc cggacttcgc ctcggtcgac acgcacggca ccccggtccg
      301 gctcgcggac ctgcgcggga cgccagtcgt cctcgtcttc gtgccgttcg cgttctccgg
      361 gacctgcacg ggtgagctgt gcgcgatccg cgacagcatc gagctgttcg acgacgccgg
      421 cgtgcgcctg ctcgtggtct cgtgcgacgc gacgtactcg ttgcgcgcat ggcaggagca
      481 ggaggggtac accttcgacc tgctctcgga cttctggccc cacggcgaga tcgcgcgaac
      541 ctacagcgtg ttcgacgacg agcgaggcgt ggcgatccgc ggcagcttcc tcatcgacgc
      601 cgacggcgtg ctgcgctggg cgctcgtcaa cccgcgcacc caggcccgtg acctcgaggc
      661 gtaccgcgcg gcgctcgccc tgctctgaca cacctgtgcg ccggtagtct ggttcaccgt
      721 cgcaggggcc cgtagctcag ttggtcagag cgccgcgctt acaccgcgga ggtcgccggt
      781 tcgagcccgg ccgggcccac ccctcaccgg catcgccctg atcgtcttca gaaaggccac
      841 cccgtgcgcg ccactgtcat ccatggtccc ttcgacgtcc gcctcgagac ggtccccgac
      901 ccgcgcatcg tcctgccgac cgacgccgtc gtgcgcgtcg tggcagcgtg cgtgtgcggc
      961 tccgacctct ggccgtaccg cggggtccgg cccaccaagt tgccgcaccg gatcggccac
     1021 gagttcgtcg gggtcgtcga ggaggtcggt gcggacgtcc gttcggtgcg ccccggccag
     1081 ttcgtgatcg cgcccttcgc gatcagtgac aacacctgcg tgcactgtcg caacggggtc
     1141 cacacctcgt gcgcgcagct cggctggtgg ggcgagccgg accacgacgg gatcccttcc
     1201 gacgccggtc agggcgagta cgtgcgcgtc ccgctcgcgg acggcacgct ggtggcgacc
     1261 ccggaccacc cggacgcggc cctgctgccc agcgtcctga cattgagcga cgtcatgggc
     1321 accggccacc acgcggcgct cgcgggcggg gtccgaccgg gctcgaccgt cgtcgtcgtc
     1381 ggtgacggtg cggtcgggct gtgcgcggtc ctcgcggcgc gacgtctggg tgccgagcgg
     1441 atcatcgcga tgtcgcggca cagcacccgc caggacctcg cggtgcggtt cggggcgacc
     1501 gacatcgtcg cggagcgcgg cgccgaggga gtggcgcgcg tcatggacct gctcgacggg
     1561 gtcggtgcgg acgccgcact cgagtgcgtc gggacgaagg agtcgatgca gcaggcgctc
     1621 gactccgcac gtccgggtgg acgggtcggg tacgtcggcg tcccggccgg tggtcccgag
     1681 ctgcccgtgc aacagatgtt ctcgtccaac gtcggcgtcc tcggcggtgt cgcgccggtg
     1741 cgcgcgtaca tctccgagct catgggcgac gtgtggtcgg gcacgatcga gccggggctc
     1801 gtcttcgacc tcgtcgtccc gctggacgac gtcgccgagg cgtacaaggc catggacgag
     1861 cggcgcgcga tcaagtcgat gctcctgccg tagcccggcg ggtagcgttc acgacgtgat
     1921 cgaccacccg acagctgctc ccacgctcgc cgaggtcgtc ggcgtgctcg accggctcta
     1981 cccacccggg acagctgagt cgtgggacgc cgtggggctc gtcaccggtg acccggcgca
     2041 gcccgtgcgt cgcgtgctct acgcggtgga cccggtcgac gccgtcgtcg acgaggcggt
     2101 cgcgtggggt gcggacctcg tcgtgaccca ccacccgctg ctgctgcggg gtgtccactc
     2161 ggtcgcggcg acgaccttca agggcgcgct gctgcatcgg ctcatccggt ccgggatcgg
     2221 gttgtacgcc gcccacacca acgccgacgc cgcaccccac ggtgtcgccg aggcgctcgc
     2281 ggccgccgtc ggtctcgtcg acctggagcc gctcgagccg atcgcggccg aggccctcga
     2341 caagcacgtc gtgttcgtgc cggtcgcgga cgcgggccgc ctggtcgacg cgctggcgct
     2401 cgcgggggcc ggggccatcg gtgcgtacac gcgctgcgcg tggaccgcga cgggcgaggg
     2461 caccttcacg ccgtcggcgg aggctcgacc ggccctgggg cgcgccggtg agatcgccac
     2521 ggtcgtcgag gcccgcgtcg agatggtcgc acctcgccgg ttgcgctccg cggtcgtcgc
     2581 ggcgatgcgg gccgcgcacc cctacgagga gccggcgttc gacgtcctcg agctcgcgcg
     2641 ctgggacggc ccgacgggga tcgga
//
LOCUS       DASRKH010000125         2627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_35673, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000125 DASRKH010000000
VERSION     DASRKH010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35673"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1275)
                     /locus_tag="VFC06_02115"
     CDS             complement(<1..1275)
                     /locus_tag="VFC06_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80726.1"
                     /translation="MTGAVASRMAPTAFAWWTSRSTRTKLLIRALVSVAVLMWVSLVL
                     AQPADAVGSLSAGTVDDETVKGFSSDVGNYLVTNRWSPVSFTPSGFLGFGSVATYGAY
                     TIQTILVGAGVFLARIFMGMIYLTSGGDLVMNSADTIDRIFAKVGNGLLGNDGGAMLD
                     GSGIMLIGVLSVILIGVTFWQYVRQGGARRAGQTFLLGFGALMLLVVMSSAAASNAKV
                     TEAAAASAGTGGRGGSTLSASMTLAHGPEPAFASPKWFITRGNVFSAFVGDTTQKIFT
                     SISDSFDASPTSSRDECERYVAAMHATYVERSGPQAATHLIVAFDQLLATTLWDNERA
                     ASMGGATESSDAAWCRYSELEASAPMIDQVQLSRKAGLYSDIVGYDASEGLAGDAPSK
                     DGTAVTAAGEWTDGGQELAADVFGPAFASGDSS"
     gene            complement(1275..1886)
                     /locus_tag="VFC06_02120"
     CDS             complement(1275..1886)
                     /locus_tag="VFC06_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80727.1"
                     /translation="MNPMQGAELGYVWLNRVFVKKATFSRIGSTDIRLSRFDVIVISV
                     LTLVFLALSIPIIYGLMKIPGLDSRVNLWPLSLLSLVAAWVAGRRLAKASPFSRFTGE
                     NIFDWAVVTADKSDTVLGRIVGHRVATNEVTTWISGQPRPVVAVEWIGSARAPDAPPR
                     TADSEDDDLVDVTLRPYVESTDWVRESRRGAQRANGFAQRSNG"
     gene            complement(2043..2426)
                     /locus_tag="VFC06_02125"
     CDS             complement(2043..2426)
                     /locus_tag="VFC06_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80728.1"
                     /translation="MNTVLMTGAMLPMAGAPTLELSVTPTTAALLRGVALFIVVAVVG
                     FVLIKKMNFMGGQRGSQGGKMQVSGIVVALLVAAILMDIDLFVTLVNLAISLVYELGQ
                     IIKGFLSDISNTGGGTVDTALDGTP"
     gene            complement(2430..>2627)
                     /locus_tag="VFC06_02130"
     CDS             complement(2430..>2627)
                     /locus_tag="VFC06_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80729.1"
                     /translation="TASNMRAAERGWDWDIFAVPEDGVDSTRTVDQDEAARQANAGLA
                     EMLDRSRRFAARGKQDSSEED"
BASE COUNT          482 a          838 c          852 g          455 t
ORIGIN      
        1 cgaagagtca cccgaggcga aggccgggcc gaagacgtcc gcagcgagtt cctgtccgcc
       61 atccgtccac tcgcctgccg ccgtgaccgc cgtaccgtcc ttgctgggcg cgtcgccggc
      121 gagaccctcg gaagcgtcgt agccaacgat gtcggagtaa aggcccgcct tgcgagacaa
      181 ctgcacctgg tcaatcatcg gagccgacgc ttccaactcg gagtagcggc accaggccgc
      241 atcgctcgac tctgtcgccc cgcccattga tgcggcccgc tcgttgtccc acagcgtcgt
      301 ggcaaggagt tggtcgaacg cgacgatgag gtgcgtggcg gcctgcgggc ccgagcgctc
      361 gacgtacgtc gcgtgcatcg ctgcgacata ccgctcgcac tcgtcacgcg aggaggttgg
      421 cgacgcatcg aaggagtccg agatggaggt gaagatcttc tgagttgtgt cgcccacgaa
      481 ggcggagaac acgttgccgc gagtgatgaa ccacttcggt gatgcgaagg cgggctccgg
      541 cccatgggcg agcgtcatcg aggcagacag cgtcgaccca cctcgcccac cagtcccggc
      601 acttgcggcc gcagcctccg tgaccttcgc gttcgacgcg gcagcggagg acatgaccac
      661 gaggagcatg agcgcgccga atccgaggag gaaggtctga cccgcacgac gcgcaccgcc
      721 ctggcggacg tactgccaga acgtcacgcc gatcaggatg acgctgagga cgccgatgag
      781 catgatgccg ctgccgtcca gcatcgcccc gccgtcgttg cccagcagcc cgttgccgac
      841 cttcgcgaag atccggtcga tcgtgtcggc cgagttcatg accaggtcgc cacccgaggt
      901 gaggtagatc attcccatga agatgcgcgc caagaacacg cctgcgccca ccaggatcgt
      961 ctggattgtg taggccccgt aggtcgcgac tgaaccaaaa ccgaggaaac ctgatggcgt
     1021 gaacgacacc ggggaccacc tgttggtgac caggtagttg cctacgtccg aggagaaccc
     1081 tttgaccgtc tcgtcgtcca cggtgcccgc agagagcgag cccacggcgt cggccggctg
     1141 cgcgagaacg agactgaccc acatcagcac cgcgacgctc acaagcgcgc ggatgagcag
     1201 tttcgtccgc gtcgagcggg acgtccacca ggcgaacgcc gtcggtgcca tccgtgaggc
     1261 cactgcgcca gtcatcagcc gttgctcctt tgtgcaaatc cattcgcgcg ttgcgcgccg
     1321 cgccgcgact cccggaccca gtccgtggac tcgacgtagg gccggagtgt cacgtcgacc
     1381 aagtcgtcgt cttctgagtc ggccgtgcgg ggcggtgcgt caggtgcgcg cgccgagcca
     1441 atccactcga cggcgactac gggccggggc tggccggaga tccatgtcgt cacttcgttg
     1501 gttgccacgc ggtggccgac gattcggccc agcactgtgt ccgacttgtc tgccgtgacc
     1561 acggcccagt cgaagatgtt ctctcccgtg aaccgcgaga agggggatgc cttcgccagg
     1621 cggcgtccgg cgacccaggc ggcgaccagg ctcagaagcg agagcggcca aaggttgact
     1681 cgcgagtcca ggcccgggat cttcatgagc ccgtagatga tggggatgga gagcgccagg
     1741 aagaccaaag tgagcacact gatcacaatg acgtcgaagc gggacaggcg aatatcggtg
     1801 gacccgatcc gcgagaatgt ggccttcttg acgaagacgc ggttgagcca cacgtagccc
     1861 aactcggcac cctgcatggg gttcattcag tcctcctcgt cgggcacatg gtagccacgg
     1921 aaaactgggt caattcgtgt ctgaatctgc gtggccgtaa acgcaacaac gggcgcccct
     1981 ttcggaggtg gccgagtggg cgcccgttgt tgcgtctagg tagggtggta tccgcccggt
     2041 cgttaagggg taccgtcaag agccgtgtcg acagtgcctc cgccggtgtt ggagatatcg
     2101 ctgaggaagc ccttgatgat ctggccgagt tcgtagacca ggctgatcgc aaggttgacg
     2161 agtgtcacga acaggtcgat gtccatcagg attgcggcga ccagcagggc cacgacgatg
     2221 ccgctgacct gcatctttcc gccctggctg ccgcgctggc cgcccatgaa gttcatcttc
     2281 ttgatgagca cgaagcccac cactgcgacg acgatgaaca gagccacgcc acgcaggagc
     2341 gccgcagtgg tgggagtcac ggagagttcc aaagtgggag cccccgccat cgggagcatt
     2401 gctccggtca ttagtacggt gttcatccct cagtcctcct cggaagagtc ttgctttccc
     2461 cgcgccgcga agcggcgtga gcggtcgagc atttcggcga gtcctgcatt tgcttgtcgt
     2521 gccgcctcgt cttggtcgac ggtgcgtgtg gaatcgacgc catcttctgg cactgcgaag
     2581 atgtcccagt cccaacctct ttcggcggcg cgcatgttgc ttgccgt
//
LOCUS       DASRKH010000126         2135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_62719, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000126 DASRKH010000000
VERSION     DASRKH010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62719"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            233..571
                     /locus_tag="VFC06_02135"
     CDS             233..571
                     /locus_tag="VFC06_02135"
                     /inference="COORDINATES: protein motif:HMM:NF020265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HZL80730.1"
                     /translation="MTSALHFGENEFITDWSKAVDTKKRRTETIQVRVTREDKDLIAR
                     AAQESDVDVSAYVTRTMRAAAQDTLAQRLHRTLSPKDFARVQEILEAPAQRIPELVAL
                     AAEPSPFADR"
     gene            581..1111
                     /locus_tag="VFC06_02140"
     CDS             581..1111
                     /locus_tag="VFC06_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80731.1"
                     /translation="MAGYTQPVALDHKRHRIDAFVSTSETQTRWLRRHGVSAHRGDFA
                     KVIVTTPVGSDSVVGYYTLHGGALSVSVAPTKLGQGNGNHDIPIALIGRLAVHVDHTG
                     QRLGRHLLLDALRRSVAIATSDLGLRGVLVNCEDAKAREFYLHVIPAFEPLPSDDLTL
                     VVSMKSLRASLTGPDN"
     gene            complement(1690..>2135)
                     /locus_tag="VFC06_02145"
     CDS             complement(1690..>2135)
                     /locus_tag="VFC06_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HZL80732.1"
                     /translation="LMLVEKFRATHGDGDDKELRAEISRAVDASPSLRNKRDLIEDFV
                     SRVTVDGAIDDEWQAFVAARREAELDAIIAAESLRADATRAFVETAFRDGALRTTGTA
                     ITKVLPPASRFSEDGGHGEKKQRVVEKLGAYFERFFGLGHDGGRD"
BASE COUNT          391 a          732 c          632 g          380 t
ORIGIN      
        1 gttcacagta gtcgcggcat gcgacatcat cccgattcgt gcaccagact caagtggcag
       61 ttcccgggac ttgaccccgt ttccactcct tcactcggag ctcgcgacaa cagagccgca
      121 tgaggcgcgc gactggccgc cgtgcacaga gcggcgtact gcgggtgacc cccacgcacc
      181 gataccgttc cgcgtcggga cgacaatatg tgtctgcggg cccggggatg tattgacatc
      241 agcactacac tttggggaga atgagttcat caccgattgg agcaaggctg tggacaccaa
      301 gaagcggcgc accgagacca tccaggttcg cgtcacgcgc gaggacaagg acctgatcgc
      361 acgggcggct caagagtccg acgttgacgt gagcgcatac gtgactcgca ccatgcgggc
      421 agctgcgcag gacacgctcg ctcagcgact ccaccgcacc ttgtcgccca aggatttcgc
      481 tcgcgtccaa gagatccttg aagcgcccgc acagcggatc cccgagctcg tggctctggc
      541 cgccgaaccc tcgccgttcg ccgaccgata gaagggccaa gtggccggct acacccaacc
      601 cgttgcactc gaccacaagc gtcaccgcat tgacgcgttc gtcagcacct cggaaacgca
      661 aacccggtgg ctgcggcgtc acggcgtctc cgcccatcgc ggtgacttcg caaaggtcat
      721 cgtcaccacc ccggtcggca gcgacagcgt cgttggctac tacacgctgc acggcggcgc
      781 gctttccgtg tccgtcgcgc ccaccaaact cggccaaggc aacggcaacc acgacatccc
      841 gatcgccctc attggccgtc tcgcagtcca tgtggaccac acaggtcagc gacttggcag
      901 gcacctactg ctcgacgcgc tccgccgcag cgttgcgatc gccacgagcg accttgggct
      961 gaggggcgtc ctggtcaact gtgaggacgc aaaggcacgc gagttctacc ttcacgtcat
     1021 accggcgttc gagccgctgc cgtcagacga cctcaccctc gtggtatcca tgaagtcgct
     1081 tcgcgcgtca cttactggac ccgacaactg acccctgtct ccgagccacc agatcgcgcc
     1141 ttgctgggtc gcatggatca acggcgatca ctctggtcag tagttgcggc cagcgagctc
     1201 ccagttcgac gagtcggaca aagcctcgcc ccacaagtac atctggaagt cctcgcagcg
     1261 aatcctggtg gacttacggc accccgctgc ctcaagaccg gtggcgaccc agtggtgcgc
     1321 ctatcgtgca ccgtgcgtgt atcgcaggcc cacttcccaa cggccgtccg aacgatgccg
     1381 cggtttactg ccttccccgt tgtcacgtcg cggtcgagtc acgaggccta ctccaagacc
     1441 aggtgaacca cgtacgcctc cactgcgcga gtggggatgc gggtgcgacc gccgatgcgg
     1501 aggcagggca ggtgcccgtc acgcagcgac gcgccgccag ggcttgggca tgctgacatc
     1561 cttccagcgg agacgaatcc ccacaggtca ggagtcaacc aaaccggggg cagtcccttg
     1621 agagctcgga aattcttgat gcgcactgac tcgatcttca ttcgatgttc cccccagtag
     1681 caaccatgtc tagtctcgac ccccgtcgtg tcccaacccg aagaacctct caaaatacgc
     1741 tccgagtttc tcgactacac gctgcttctt ctcgccgtga ccgccgtcct cggagaatcg
     1801 cgacgctggc ggcagcacct tcgtgatcgc cgtacctgtc gtgcggagtg cgccgtcacg
     1861 gaatgcggtc tcgacgaatg cgcgggtggc gtcagcgcgc aggctttcgg cggcgatgat
     1921 tgcgtcgagc tctgcctcgc gtctcgcggc gacgaaggcc tgccactcgt cgtcgatcgc
     1981 cccgtcgacg gtgacacggc tgacgaagtc ctcgatgagg tctcgcttgt ttcgcagcga
     2041 cgggctggcg tcaacggcgc gggagatttc ggcgcgcagt tccttgtcgt cgccgtcgcc
     2101 gtgggtggcg cggaacttct cgaccagcat gagga
//
LOCUS       DASRKH010000127         2372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_2987, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000127 DASRKH010000000
VERSION     DASRKH010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2987"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1278..1703
                     /locus_tag="VFC06_02150"
     CDS             1278..1703
                     /locus_tag="VFC06_02150"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZL80733.1"
                     /translation="MTKDRQRFIAHGLVERDGAFLVLRRRDGRYFGGQWDIPGGTVEA
                     GETPAVAAARECLEETGLRATVGPKLSHFQNRDTEGGDLTFNTVTFRLHLVDEDTEVR
                     LSPEEHDDYRWMQPTNPDGLPLVWHVGRTLGVLTLHQQR"
     gene            complement(1779..>2372)
                     /locus_tag="VFC06_02155"
     CDS             complement(1779..>2372)
                     /locus_tag="VFC06_02155"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HZL80734.1"
                     /translation="VYGVPKPLGITEDRREVLSFVEGMVPTYPMPGWAWAETALESSA
                     RLLRQVHDATAGVDLQGPWRSPVREPVEVVCHNDFAICNLVFDGEAVVGVIDWDFASP
                     GPRLWDIAYLAYRIVPLSSGDRRDGFTDEERDRRLERLLDAYGCDAKPRDVITVLHER
                     LLALAAFSDDMAERLGKPELGEHASLYRHDAAHLPAT"
BASE COUNT          375 a          783 c          809 g          405 t
ORIGIN      
        1 gtacgggggc cgactgtggg ctcctgctgg tctgtggggt gtcctgcgac atggcggtca
       61 tcctttcaaa ggctccgttt accctctaac catgacccgg atcacacggg cccgcaaccg
      121 ggggccccca ccgtccagag gccgtccgga cgtcgcttgt cggtcgggag ctcgcgccac
      181 tagtttgttg tgtttgctgt tcccccacgc gacgactgtc aacgaccaag agtcactggg
      241 tgaaagggga gtgaggctcc cagctcaccc gccgtgcctt ctgtgggcgg cggtttcccc
      301 gcgagtccag accttcctcc gtggcctgca gggcggccgg taggcccgcc ccggggcaag
      361 tctcagaacg atcgccaagt gaggagacct cgggcaggtg cgagatcttc accaggttct
      421 cggccgctcg acctgaatct cctgggtctc gaggtggtgg gagctgcccg gggtcgtact
      481 cgttcttcgg tgagggcggc tggagccctt cgtttccttg cgtactacca ggacgcccgc
      541 ctcgcccact ggcgcgttgc ttccgattgg cagcagcctg ttcacttgcg ccacgatcag
      601 cgagaccacc aggactccag ccgcgttgtt cggctgtttg tgtcggacct tcttcccgga
      661 gccccggccc ttggcgctct gcgtgaagga tgcctatgtg cgagagcgtg cggtgtgtgt
      721 gatccgtgcg gcgagcctct ccacccgctc tcgggtggcg cctcgcgcta gatcgggtag
      781 cttgtggtcg agggagaccg ccgtgccggt aggcgcggcg gggatgaggg cggggagtcc
      841 ctcaccccct tcccgcacgg cggcctggcc aggcaggaaa aatgcccggg gccggccgcc
      901 gtgcgggagt acatagagcc gcgagctcgc acagcgacga tgcacgcccc gcgcgcgttt
      961 ccaccgccac cgaatcgagc ccggcggggt gaccgggccg tgcccgcgtg gacgccatcg
     1021 cctcacccag caccagggcc tcgacccgct ccgcgtgccg ggcggccagg acaagggcga
     1081 cggcggcctt gtccagccag cccaggacgc cgcgcgcgtg ctgccagccg cgtcgagccc
     1141 ggcgagcagg tcggggacaa ggtcgtcaat cggacatccc ggtaccccgc gcgtcgtaga
     1201 ggatcaggcg gcccatgctc gcgagagggt gtccagccaa gaccgagaag tgtgtccctg
     1261 gtggcaagat gcgttccgtg accaaggaca ggcagcgctt catagcgcat gggctggtcg
     1321 agcgcgacgg tgcttttctc gttctacgcc gtcgtgacgg tcgctacttc ggaggccagt
     1381 gggacatccc gggaggcacc gtcgaagcgg gagagacccc ggctgtggcg gcagctcgcg
     1441 agtgcctgga agagactgga ctgcgcgcca ctgtggggcc gaagttgtcg cacttccaga
     1501 accgcgacac cgagggcggc gacctcacgt tcaacacggt gacgtttcgg ctgcacctcg
     1561 tcgatgaaga caccgaggtc cgcctgagtc ccgaggagca tgacgactac cggtggatgc
     1621 agccaaccaa ccccgacggg ctgccgctgg tctggcacgt cggaaggacc ctcggcgttc
     1681 tgacccttca tcaacagcgt tgaaccacaa tgtgccgtcg cggacgcatc atctgccgcg
     1741 tctagtgcgg tccatcgtcc cgacgaccgt aggttcggtc aggtcgcggg gaggtgggcc
     1801 gcgtcgtgcc gatagagact ggcgtgttcg cccagttctg gcttgcccag tcgctccgcc
     1861 atgtcgtcgc tgaaggcggc gagagcgagc aggcgctcgt gaagcactgt gatgacgtcg
     1921 cgcggtttgg cgtcgcagcc gtaggcgtca aggagccgtt ctaggcgacg gtcgcgttcc
     1981 tcgtctgtga atccgtctcg ccgatcgcca gacgagagag ggacgatgcg ataggccagg
     2041 taggcgatgt cccacaggcg agggcccggc gaagcgaaat cccagtcgat gacgccgacg
     2101 acggcttccc cgtcgaagac caagttgcag atggcgaagt cgttgtggca tacgacctcg
     2161 accggctcgc gtaccgggga ccgccacggg ccttgcaggt ccacacctgc ggtcgcgtcg
     2221 tgaacctgcc gaagcaagcg cgcggaggac tccagcgcgg tctcggccca ggcccaccca
     2281 ggcatcgggt aggtcggcac catcccctct acgaaactga gcacctccct ccggtcctcg
     2341 gtaatgccga gtggctttgg aacgccgtac ac
//
LOCUS       DASRKH010000128         2682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_14324, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000128 DASRKH010000000
VERSION     DASRKH010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14324"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1186
                     /locus_tag="VFC06_02160"
     CDS             <1..1186
                     /locus_tag="VFC06_02160"
                     /inference="COORDINATES: protein motif:HMM:NF015706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HZL80735.1"
                     /translation="IKTTTDPFVGRVSVVRVYSGVLRSDQMVHISGHLSQFSGKPANP
                     QWHADHDEEERVGALGRPLGGSQVPVDKAVAGEVVTVARLTKAETGDTLSDPANPAVI
                     TPWTMPEPLYPTAILNLKKGDEGKLMQGLQRLQAEDPTVRVEVDATTGQLVLWTMGEQ
                     HLQVMLDRLRNRSGVEVKTVPVLVALRETLKDKCEVQGKHVKQSGGHGQYAVADLRFE
                     PLPQGSGFEFVDEVVGGTVPRQYIPSVEKGIVNQMAKGITGYPMVDVRAVLYHGKAHA
                     VDSSDAAFQMAGALSVQEAAKKGGITILEPVDTVTVECDDEFVGGVMSDLSTRRGRVK
                     GTDQVGEGRTAVTAEVPTFELVRYATALRSLARGTGTFSREYLHHEPAPSHLTEKLVA
                     GA"
     gene            1404..1856
                     /locus_tag="VFC06_02165"
     CDS             1404..1856
                     /locus_tag="VFC06_02165"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZL80736.1"
                     /translation="MTYASQVRARREALHLSQRELAARSGVKQPLISAIESGRRQPSA
                     ATRTALDRVLVVRPSQVLREMRGQALEIVARHHGSAPRVFGSVARGTDGPDSDVDLLV
                     TFPEDAGITDLLAMEDELTELLTVRVEVVSAGSGGRVIDRAVREAIPL"
     gene            1853..2215
                     /locus_tag="VFC06_02170"
     CDS             1853..2215
                     /locus_tag="VFC06_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HZL80737.1"
                     /translation="MTPSEHPVPASRLRVWLDDLAGFGRDADYLVALGRDAYLADTAQ
                     GRLLRNAGERLLIKVATVVERFPEDFKSDHHNIEWVDITRMRNLIAHQYDKVNHDLMW
                     VTLQRDIPALMERLGLSA"
     gene            complement(2336..>2682)
                     /locus_tag="VFC06_02175"
     CDS             complement(2336..>2682)
                     /locus_tag="VFC06_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80738.1"
                     /translation="RATPRALAQRPALPRNFEQLATPVVAHFRIGVAKERIQPRFWFP
                     NRQLAPSMRLGMLIEGSNRPKPGSSALRGRIRRFHERSVDSLGCVEARAHVSVFSREC
                     SLQNPPRVLPGG"
BASE COUNT          432 a          916 c          927 g          407 t
ORIGIN      
        1 catcaagacc accacggacc cgttcgtggg ccgcgtctcc gtggtgcgcg tctactccgg
       61 cgtgctgcgc tccgaccaga tggtgcacat ctccggccac ctgtcccagt tctccggtaa
      121 gcccgcgaac ccgcagtggc acgccgatca cgacgaagag gagcgcgtcg gcgccctcgg
      181 ccggccattg ggtgggtccc aggtgccggt ggacaaggcc gtggcgggtg aggtggtcac
      241 cgtggcgcgg ctgaccaagg cggagacggg cgacaccctc tccgacccgg cgaaccctgc
      301 cgtcatcacc ccgtggacga tgcccgagcc gctgtacccc actgcgatcc tgaacctgaa
      361 gaagggcgac gagggcaagc tcatgcaggg cctgcagcgc ctccaggccg aggaccccac
      421 ggtccgcgtg gaggtggacg ccaccaccgg gcagctcgtg ctgtggacca tgggcgagca
      481 gcacctccag gtgatgctcg accggctgcg gaaccgctca ggtgtcgagg tcaagaccgt
      541 tccggtgctc gtggccctcc gcgaaaccct caaggacaag tgcgaggtgc agggcaagca
      601 cgtcaagcag tccggtggcc acggccagta cgcggtggcg gacctccgct tcgagcccct
      661 gccccagggc agcggcttcg agttcgtgga cgaggtggtg ggcggcaccg tgccgcgcca
      721 gtacatcccg tccgtggaga agggcatcgt gaaccagatg gcgaagggca tcaccggtta
      781 cccgatggtg gacgtccgcg ccgtgctgta ccacggcaag gcccacgcgg tggactcctc
      841 cgacgccgcc ttccagatgg ccggcgcgct cagcgtgcag gaggccgcga agaagggcgg
      901 gatcaccatc ctcgagcccg ttgacacggt gacggtggag tgcgacgacg agttcgtcgg
      961 cggcgtgatg tccgacctct ccacccgccg tgggcgcgtc aagggcaccg accaggtcgg
     1021 tgaaggacgc accgcggtca ccgcggaggt tcccacgttc gagctggtcc gctacgccac
     1081 cgcgttgcgc tcgctcgccc ggggcaccgg caccttctcg cgggagtacc tgcaccacga
     1141 gccggcgccg tcgcacctga cggagaagct ggtggccggg gcgtaggccg caaccgctgg
     1201 caaccgatac gccccccggc gggtgtatcg ccaattgcga gttattgcgg cttcgagcgc
     1261 ccaatcggcc ccaattgagg ccatcaagcg atacagccgg ggcgagccgg cccggactgt
     1321 atcggttgcc aacgtgttgc cagccgtcgc attcgcgcac caaacatctt ggtccgcgat
     1381 aacaccggcg ttatcatgtg ggtgtgacgt acgcgagcca ggtcagggca cgtcgggagg
     1441 cgcttcacct ttcgcagcgt gagttggcgg cgcgctcggg tgtgaagcag ccgctgattt
     1501 cggcgatcga atccggcagg cgtcagccca gcgccgcaac ccggacggcg ctggaccggg
     1561 tgctggtggt ccggccgtcc caggtgctac gggagatgcg cgggcaagcg ctggaaatcg
     1621 tcgcgcggca ccacgggagc gcaccgcgag tgttcggttc ggtggcgcgg ggtacggatg
     1681 gtccggactc cgacgtcgac ctgctggtca ccttccccga ggacgccggg atcaccgacc
     1741 tgctggccat ggaggacgaa ctcactgagc tcttgacggt tcgggttgaa gttgtgtcag
     1801 ccgggtcggg cggacgggtc attgaccggg ccgttcgcga ggcgatcccg ctgtgacgcc
     1861 ctcggagcac ccggtccccg cgagccggct ccgcgtctgg ctcgatgatc tggccgggtt
     1921 cggccgagac gccgactacc tggtcgcctt gggacgcgat gcctatctgg ccgacaccgc
     1981 gcagggccgg ctgctgcgga acgccggcga gcgcctgctc atcaaggtgg cgacggttgt
     2041 ggagcggttt cccgaggatt tcaaatcgga ccaccacaac atcgagtggg tcgacatcac
     2101 gcggatgcgg aacctgatcg cccaccagta cgacaaggtc aaccacgacc tcatgtgggt
     2161 caccctccag cgcgacatcc cagcgttgat ggaacggctc ggcctgtccg cctgaccggt
     2221 gcccgctcgc ggccgccgtg cctgcaggag catgctgtcc tcgcccgcgt ccggcccacc
     2281 acctcatctg aagcgccgtc cgccgcctcc gccagccgcc tgcccctcgc ggtcctcagc
     2341 ctcccgggag aactcgaggc ggattctgca acgagcactc ccgggagaat acggacacgt
     2401 gcgcacgcgc ctcgacacag cccagagagt ccaccgaccg ctcgtggaac ctccggatgc
     2461 gccccctcag cgcgcttgag ccgggtttcg ggcggttgga gccctctatg agcatgccca
     2521 gccgcatcga gggcgccaac tgccggtttg ggaaccagaa tcgcggctga atacgttcct
     2581 tggcgacgcc gatgcggaaa tgggccacca ccggcgtcgc caactgctcg aaattgcgcg
     2641 ggagcgcggg gcgttgggcg agggcgcggg gcgttgcgcg gg
//
LOCUS       DASRKH010000129         3522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_41089, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000129 DASRKH010000000
VERSION     DASRKH010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41089"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="VFC06_02180"
     CDS             <1..774
                     /locus_tag="VFC06_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HZL80739.1"
                     /translation="VLLRRGQIPTTTSDQRLLSGDGDTSWLHDDPWRVMRIQSEFVEG
                     FGALAEIGPAVSVFGSARVAIDHPDYAIGEAVGRGLVEAGYAVITGGGPGIMAAANKG
                     ASQAGGLSIGLGIELPFEQGMNQWVDLGISFRYFFARKMMFVKYAQGFVVLPGGFGTL
                     DELFEALTLVQTHKVTSFPIVLIGSSYWSGLLEWIAGPVVDRGMINPADLELLKIVDD
                     ADEAVRIVCERGAELRAQELDAVRREADARRRAAEGNGS"
     gene            788..1738
                     /gene="folP"
                     /locus_tag="VFC06_02185"
     CDS             788..1738
                     /gene="folP"
                     /locus_tag="VFC06_02185"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HZL80740.1"
                     /translation="MMGAVIGGIPAAGADVRLRGRTFGPDHPVVMAVVNRTPDSFYAP
                     ARHDEAGADAAVARAVEEGADLVDLGGVRAGRGPRVDVAEEIARVVPLIERVRARHPD
                     LLVSVDTWRAEVARAAALAGADLLNDTWAGHDPQLVEVAAEFGIGVVCSHTGGARPRT
                     DPFRVAYPRRDPGAGLPTESADPLDGVVDDVVRVLSAAAARAVGLGIDPAGVLVDPTH
                     DFGKNTWHSLHLVRRTQALVALGHPVLVALSRKDFVGETLDLPPDERLEGTLAATSVA
                     AWLGARVFRVHDVRATRRTLDMIAAIRGDAVPARAVRGLA"
     gene            1735..2994
                     /locus_tag="VFC06_02190"
     CDS             1735..2994
                     /locus_tag="VFC06_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80741.1"
                     /translation="MSAQAVDVAERTPGSVWSARVDPRRWPWWGRALGAYLATRVFSA
                     VVLVVVAQFQVENYWTPAKPSYLRFTGLMWDATWYRTIAEQGYPAVLPHGADGLVQQN
                     PWAFFPLFPFLVRGVMILTRAPWYVTAPLVALALGALAAVVIYRLIERGAPRAVAERP
                     GLPLATVVLLGLFPSAAVLQIGYTESLALLLVAASLLAIVERRYAWASLAVLALGFTR
                     AVALPMAVVVVVHGLVRWRASRRGEDRLTLPDVAGLGLLAGTAAISGVAWPLICGWVT
                     GEPDGYLQTQGSWRGVRDIQPFVPWRYVSRFWFGDWAPLVLVVGFGLVIACLVVPAAW
                     RLGNELHVWSAAYLLYIVGAIEPGSSLARFLLLAFPLGAVAAGLVTRPPWRRRVWFGL
                     VAALMAGLQIVWIYNTWRLTPPSGWPP"
     gene            3192..3359
                     /locus_tag="VFC06_02195"
     CDS             3192..3359
                     /locus_tag="VFC06_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012877752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3117 domain-containing protein"
                     /protein_id="HZL80742.1"
                     /translation="MAAMKPRTGDGPLEVTKEGRGIVMRVPLEGGGRLVVELNATEAR
                     ELGEALTSVVS"
BASE COUNT          404 a         1220 c         1331 g          567 t
ORIGIN      
        1 gtgctcctgc gccgcggcca gatcccgacg acgacgtccg accagcggct gctctcgggc
       61 gatggcgaca cgagctggct ccacgacgac ccgtggcggg tgatgcgcat ccagagcgag
      121 ttcgtcgagg gcttcggggc gctcgccgag atcggacctg cggtcagcgt gttcggttcg
      181 gcccgggtcg cgatcgacca cccggactac gcgatcggtg aggccgtggg gcgggggctg
      241 gtcgaggcgg ggtacgcggt catcaccggc ggtggcccgg gcatcatggc cgccgcgaac
      301 aagggcgcca gtcaggccgg ggggctgtcc atcggcctcg ggatcgagct gccgttcgaa
      361 cagggcatga accagtgggt cgacctcggg atcagcttcc ggtacttctt cgcgcgcaag
      421 atgatgttcg tcaagtacgc ccagggcttc gtggtgctgc caggcgggtt cgggacgctc
      481 gacgagctgt tcgaggcgct cacgctcgtg cagacccaca aggtcacgag cttcccgatc
      541 gtcctcatcg gcagctcgta ctggtccggt ctgctcgagt ggatcgccgg ccctgtggtc
      601 gaccgcggca tgatcaaccc ggccgacctc gagctcctca agatcgtcga cgacgccgac
      661 gaggccgtgc gcatcgtgtg cgagcgaggc gccgagctgc gcgcccagga gctcgatgct
      721 gtgcggcgag aggccgacgc tcggcggcgg gcagccgagg gcaacggctc gtgaccgtcg
      781 cggcgcgatg atgggcgcag tcatcggcgg catccccgcc gcgggagctg acgtgcgcct
      841 gcgcgggcgg acgttcggcc cggaccaccc cgtcgtgatg gcagtcgtca accgcacgcc
      901 ggactcgttc tacgcaccgg cacgccatga cgaggcgggt gccgacgccg cggtcgcgcg
      961 cgccgtcgag gagggtgccg atctcgtcga cctcggtggg gtccgggccg gtcgcggtcc
     1021 ccgcgtggac gtcgcggagg agatcgcccg tgtcgtgccg ctcatcgagc gcgtccgtgc
     1081 acgccacccc gacctgctcg tgagcgtcga cacgtggcgg gccgaggtcg cgcgagccgc
     1141 tgcgctcgcg ggggccgacc tgctgaacga cacctgggca ggccacgacc cgcagctcgt
     1201 cgaggtggcc gccgagttcg gtatcggcgt cgtctgctcg cacaccggcg gcgcaaggcc
     1261 gcggaccgat ccgttccggg tcgcgtaccc gcgtcgggac cccggtgccg ggctcccgac
     1321 cgagtcggcg gacccgctcg acggcgtggt cgacgacgtc gtgcgggtgc tctccgccgc
     1381 ggccgcgcgt gcggtcgggc tcgggatcga cccggcaggc gtcctggtcg acccgacgca
     1441 cgacttcggc aagaacacct ggcactcgtt gcacctggtg cggcgcacgc aggccctcgt
     1501 cgcgctcggt caccccgtgc tggtcgcgct ctcccggaag gacttcgtgg gggagaccct
     1561 cgacctgccg cccgacgagc gactcgaggg cacgctcgcg gccacgtcgg tcgcggcctg
     1621 gctcggtgcc cgggtgttcc gcgtgcacga cgtgcgcgcg acccgtcgga cgctcgacat
     1681 gatcgcggcc atccgcgggg atgcggtccc cgcccgagct gtgcggggac tcgcgtgagt
     1741 gcacaggctg tggacgtcgc cgagcggacg cccgggtcgg tgtggtcggc aagggtcgat
     1801 ccgcgccggt ggccgtggtg gggtcgcgcg ctcggtgcgt acctcgcgac gagggtgttc
     1861 agcgctgtcg tgctcgtggt cgtcgcgcag ttccaggtcg agaactactg gacgcccgcc
     1921 aagccgtcgt acctgcgttt caccggtctg atgtgggacg cgacctggta ccgcacgatc
     1981 gctgagcagg gctacccggc ggtgctgccg cacggcgcgg acggactggt gcagcagaac
     2041 ccgtgggcgt tcttcccgct gttcccgttc ctcgtgcgcg gggtcatgat cctgacgcgg
     2101 gcgccctggt acgtcaccgc gccgctcgtg gcgctcgcgc tcggggcgct cgccgccgtc
     2161 gtgatctacc ggctcatcga gcggggcgcg ccgcgcgccg tggcggagcg cccgggcctt
     2221 cccctggcga ccgtcgtgct gctcgggctg ttcccgtccg ccgcggtcct gcagatcggg
     2281 tacaccgagt ccctcgcgct gctgctcgtc gcggcgtcgc tgctggcgat cgtcgagcgc
     2341 cggtatgcat gggcctcgct cgccgtgctg gcgctcgggt tcacgcgtgc ggtcgctctc
     2401 ccgatggcgg tcgtcgtcgt cgtccacggt ctggtgaggt ggcgggcgag ccggcgcggc
     2461 gaggacaggc tgaccctgcc cgacgtcgcg gggctggggc tgctcgccgg caccgcggcg
     2521 atctccggcg tcgcatggcc cctgatctgc ggctgggtga ccggcgagcc ggacgggtac
     2581 ctgcagaccc agggctcgtg gcgcggggtc cgagacatcc agccgttcgt cccgtggcgc
     2641 tacgtgtccc ggttctggtt cggcgactgg gcgccgctcg tcctcgtcgt cggcttcggg
     2701 ctcgtcatcg cgtgcctcgt cgtcccggcg gcctggcgac tcggcaacga gctgcacgtg
     2761 tggtccgcgg cctacctgct gtacatcgtc ggggcgatcg agccgggcag cagcctcgcc
     2821 cgcttcctgc tgctcgcgtt cccgctcggt gcggtggccg ccgggctcgt gacgcggccg
     2881 ccgtggcgtc gacgcgtctg gttcggtctc gtcgccgcac tcatggccgg gctgcagatc
     2941 gtctggatct acaacacctg gcggctgacc ccgccgagcg ggtggccgcc gtgagctgcg
     3001 cgcggcactg acgtccgcgc tcggtgctct cacctgggtg ggtgcacgtc tcgaccccag
     3061 caggcagtgc gggtgaacta gactgctccg tggacatccg gttgctgagg ccgtcgacgt
     3121 gcgcgctcac cctcgggcag agcgctcacg cgatgagcga ccggtcgaag cgacgttaag
     3181 gagatccccg catggccgcg atgaagccga ggaccggtga cggcccgctc gaggtcacca
     3241 aggaggggcg gggcatcgtc atgcgcgttc cgctcgaggg tggcggccgt ctggtcgtcg
     3301 agctcaacgc caccgaggcg cgtgagctgg gcgaggccct gacgtctgtc gtcagctgac
     3361 acgttggcac gcgtgagggg tccgctggcg cacacgagca tcgggcgggc gttgcctgcg
     3421 gtggtcgtcg aggtcgggtc ggtcgcgggg acagccctgc tgctcgagcc ggacgtcgac
     3481 gcgctcgctg tgccggtcgc gcctgccgtg ccgggggaga gt
//
LOCUS       DASRKH010000130         2169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_29848, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000130 DASRKH010000000
VERSION     DASRKH010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29848"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VFC06_02200"
     CDS             complement(<1..552)
                     /locus_tag="VFC06_02200"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482342.1"
                     /note="catalyzes the formation of L-threonine from
                     O-phospho-L-homoserine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HZL80743.1"
                     /translation="MRYISTRQRPGDHPLAFCDILLEGLAPDGGLYVPDAYPRVDGET
                     LDRWRAVLADGGYPALAHAVLGLFIDDIPSDDLLAICRRAYTVEKFGTEEIVPVREIG
                     DGLWLARLSGGPTAAFKDMAMQLLGELFEYELGRRGTVLNILGATSGDTGSAAEYAMR
                     GKRGITVFMLTPRGRMTPFQQAQV"
     gene            complement(641..1114)
                     /locus_tag="VFC06_02205"
     CDS             complement(641..1114)
                     /locus_tag="VFC06_02205"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HZL80744.1"
                     /translation="MTTTLEAQTTPQIFELHTTGAWQGQFRTDVAVRGFGFTVAEPEV
                     LGGDNSGPNPLEYVLGGFEGCIAVVVEVVTRERGITLNDLRLNISGSIDLRGFGGVPG
                     VSPSFLTIDGAVHVFADIDEPTFRDLIDETERRCPLFNLFRDAGVTPDIRWSLNI"
     regulatory      complement(1383..1474)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            1759..>2169
                     /locus_tag="VFC06_02210"
     CDS             1759..>2169
                     /locus_tag="VFC06_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80745.1"
                     /translation="MLALAIAVGLELVHTVNDPWKEPLERFTALQADVVLLGALIVWA
                     LLVFLLALTGRLWISAGVTLTLATMVGFADYQKMRLRSEPLYPSDVTYLGQFRFLVES
                     VGTGRALGVAAALLAIPAVAWGVARLVGRRMRPGM"
BASE COUNT          301 a          723 c          820 g          325 t
ORIGIN      
        1 cacctgcgcc tgctggaacg gcgtcatgcg cccgcggggc gtcagcatga agacggtgat
       61 gcccctcttg ccgcgcatgg cgtactcggc cgcggagccg gtgtccccgc tggtggcgcc
      121 caggatgttg aggaccgtgc cgcggcgccc cagctcgtac tcgaacagct cgccgaggag
      181 ctgcatcgcc atgtccttga aggcggcggt ggggccgccc gagaggcggg cgagccacag
      241 tccgtcgccg atctcccgca ccgggacgat ctcctcggtg ccgaacttct ccacggtgta
      301 cgcccgccgg cagatggcga gcaggtcgtc ggaggggatg tcgtcgatga agaggcccag
      361 caccgcgtgc gcgagggcgg ggtagccgcc gtcggcgagg acggcgcgcc agcggtcgag
      421 cgtctcgccg tcgacgcgcg ggtaggcgtc cgggacgtag agcccgccgt cgggcgcgag
      481 gccctcgagc aggatgtcgc agaacgcgag cgggtggtca ccgggccgtt gacgggtcga
      541 gatgtagcgc accctcccag cgtaggtcag cgcgtgggga cgttggggtc acggggcccc
      601 gccggctctc caccagcggg gccccggtcc aacgcgggcg tcagatgttc agcgaccacc
      661 ggatgtccgg cgtcacgccg gcgtcacgga agaggttgaa cagggggcac cggcgctcgg
      721 tctcgtcgat gaggtcgcgg aacgtgggct catcgatgtc cgcgaacacg tggacggcgc
      781 cgtcgatcgt caggaacgac ggcgaaaccc ccgggacccc gccgaaaccg cgcaggtcga
      841 tggaccccga gatgttcagc cggaggtcgt tgagggtgat accgcgctcc cgcgtgacca
      901 cctcgacgac gaccgcgatg cagccctcga acccgccgag gacgtactcg agggggttgg
      961 ggccggagtt gtcgccgccg agcacctcgg gctcggccac ggtgaagccg aagccgcgga
     1021 cggcgacgtc ggtgcggaac tggccctgcc aggctcccgt ggtgtggagc tcgaagatct
     1081 ggggggtggt ctgggcctca agggtggtgg tcatggtgca ctgctttctc tggcggcacg
     1141 ccggggcacg ggtcggctcg acgggccaag gattgggaac gcctcgtgga ggcggaattg
     1201 gcggcggtca cccgcccgtg gtgccctctc ggcgccggtg gcgcggactc gatcgtcagg
     1261 gcacgtgcgg cctcaagccg cggcgcacat tcggaggagc cgcgccgacc tacagtgcat
     1321 cgtcgcgcgg cgtgccacag tctgggcggt ggggtactcg tcgaggtggc gagtcatggg
     1381 atcctccatc gatcctggca ttagcaccgg tccgcaggag cggcggttgc tgcagcgtca
     1441 gggagccagg tctctcagct gctctggata ggtgctgtaa ccgtagggcc aaaggcccac
     1501 gctccgcacc aacctgtcca catcctggga ccggcggctg gagagggttt gcgccgaggg
     1561 taagggtttg gagcccgccc agcgcacgtt cacggccccg gacaggcggg aatagaatcg
     1621 actgccatga acccgtcgcc gcgccacgcg tccccgaggg gtcgggatac gggacctgac
     1681 gccgggggac cgccgcgccg cgcgccgtcg tcgccccggc ttggggggcg aatcgcgctg
     1741 tacctggccg cgagcctggt gctcgcactc gcgatcgcgg tgggcctcga gctggtgcac
     1801 accgtcaacg acccctggaa ggagccgctc gagcggttca ccgcgttgca ggcggacgtg
     1861 gtcctgctcg gtgcgctgat cgtgtgggcc ctcctcgtgt tcctcctcgc cctcaccggg
     1921 aggctgtgga tctcggccgg cgtcaccctc acgctggcca ccatggtcgg cttcgcggac
     1981 taccagaaga tgcggctgcg gagcgagccg ctctatccct ccgacgtcac ctacctgggc
     2041 cagttccgct tcctggtcga gagcgtgggg accgggcgcg cgctcggcgt ggcggctgcg
     2101 ctcctcgcga tccccgccgt cgcctggggg gtggcgcggc tggtggggcg ccgcatgcgc
     2161 ccggggatg
//
LOCUS       DASRKH010000131         2791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_41161, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000131 DASRKH010000000
VERSION     DASRKH010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41161"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..344
                     /gene="rpmB"
                     /locus_tag="VFC06_02215"
     CDS             153..344
                     /gene="rpmB"
                     /locus_tag="VFC06_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HZL80746.1"
                     /translation="MAANCDVCAKGPSFGNSVSHSHQRTRRRWNPNIQRVRAVVAGTP
                     KRLNVCTSCLKAGKVQRAV"
     gene            390..956
                     /locus_tag="VFC06_02220"
     CDS             390..956
                     /locus_tag="VFC06_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZL80747.1"
                     /translation="MGLRAAHPVWHRERMVGQLDEVTIRTAAEADAVAIANVHVHSWR
                     EAYEGIVPAEHLASLDPVARAAEWTQFLRNGPHDHVRTWLAESPSGVLGFVTLGPARD
                     EDANRGDHEIYSIYLEPGAWGHGVARDLLRTVITEVGGRSALTLWVLAANDRARHFYR
                     RNGFQADGTERFEDVGGAELLEVRYRRG"
     gene            complement(941..1993)
                     /locus_tag="VFC06_02225"
     CDS             complement(941..1993)
                     /locus_tag="VFC06_02225"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HZL80748.1"
                     /translation="MSSPDEPTVADLSEDALLALIFPQLPLGDATLVPPGDDAAVVAA
                     PDGRVVVSTDVLVEDRHFRRRWSTGEDVGRRAAAQNLADIAAMGARPTALVVALVAPA
                     DLPASWVEGLARGLAAVCAPLGVGVVGGDLSSGPVAVVAVTVHGDLAGRDPVLRSGAR
                     PGDVVAHAGVRGWSAAGLALLDAGRGELDAGLVGAYLRPEPPLAAGPLAASAGATAML
                     DVSDGLLRDAGRIARASDVVLDLDDPAEAFAADLARLAGAADALGAQARDWVLTGGED
                     HGLLATFPAGTAVPAPFRVVGQVRAVPAGDQEAAQVTRVLVAGVAPGPGSPGWDHFAR
                     ESAAGQPGLAAGQPRR"
     gene            2081..2638
                     /locus_tag="VFC06_02230"
     CDS             2081..2638
                     /locus_tag="VFC06_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011692354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3515 family protein"
                     /protein_id="HZL80749.1"
                     /translation="MTDLPTRSAGTPGHVGHDRRTPVASPRHARRLPAAAGLVLALAA
                     CAPGVSVAEAPHGADPVCASVVLALPDRLGDLPRQRTTSQASAAWGTAAAPVVLRCGV
                     EVPGPTTVHCVSVETPDGPSIDWLAVADDPAAQGTTDWTFTTYGRDPAVEVRVPGSVA
                     STRSTSFLDQLGPAIAQVEPTRSCL"
     gene            complement(2664..>2791)
                     /locus_tag="VFC06_02235"
     CDS             complement(2664..>2791)
                     /locus_tag="VFC06_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase A"
                     /protein_id="HZL80750.1"
                     /translation="TMPGFTPFSMYPRMWQATGLSYPDLIDELLQLALHRPTGLR"
BASE COUNT          390 a         1086 c          994 g          321 t
ORIGIN      
        1 ggctagtctt tccaggttgc ccggccgtgt ccgggcgcag gtggcgcacc cgtgctcgac
       61 ggacagcatc cgctggacgg cagcgtgtcg cgccccaccc cagaccaaga caggccgctc
      121 tgcggcgtgc atgaccgaac caggagaaga ccgtggctgc taactgcgac gtctgcgcca
      181 agggcccgag cttcgggaac agcgtctcgc actctcacca gcggacccgt cgccgctgga
      241 acccgaacat ccagcgcgtg cgtgccgtcg ttgccggcac cccgaagcgg ctgaacgtct
      301 gcacgtcgtg cctcaaggcc ggcaaggtgc agcgcgcggt ctgaccgcca ccacagactc
      361 gacccgaagg cccgcgaggt tcgttcctcg tgggccttcg tgctgcccac ccggtgtggc
      421 atcgtgaacg catggtcgga cagctcgacg aagtgacgat ccggactgct gccgaggcag
      481 acgccgtggc gatcgcgaac gtccacgtgc actcctggcg ggaagcctac gagggcatcg
      541 tccccgccga gcacctggcc tcgctcgacc cggtcgcacg cgccgccgag tggacgcagt
      601 tcctgcgcaa cggaccgcac gaccacgtgc ggacgtggct tgccgagtcg ccgtcgggcg
      661 tcctcggctt cgtcacgctg ggaccggccc gcgacgagga cgcgaaccgc ggcgaccacg
      721 agatctactc gatctacctc gagcccggcg cgtgggggca cggggtcgcc cgtgacctcc
      781 tgcgcacggt catcaccgag gtcggcggcc gatcggcgct gaccctgtgg gtgctcgcgg
      841 cgaacgaccg ggcacgccac ttctatcgcc gcaacggctt ccaggccgac ggcaccgaac
      901 gcttcgagga cgtcggcggc gccgagctcc tcgaggtgcg ctaccgccgt ggctgacccg
      961 cagccagccc cggctggccc gccgcggact cgcgcgcgaa gtggtcccag cccggtgagc
     1021 ccggcccggg cgcgacaccc gcgacaagca cgcgggtgac ctgcgcggcc tcctgatctc
     1081 ctgcgggcac cgcacggacc tgacccacga cccggaacgg cgccggcacc gccgtgccgg
     1141 cagggaacgt cgcgagcagc ccgtggtcct ctcccccggt caggacccag tcacgtgcct
     1201 gggcgccgag cgcatccgcg gcacccgcca gacgcgcgag gtcggccgcg aacgcctcgg
     1261 ccgggtcgtc gaggtcgagc acgacatcgc tcgcgcgcgc gatccggccg gcgtcgcgca
     1321 gcagcccgtc ggacacgtcg agcatcgcgg tggcacccgc gctcgcggcg agcggccccg
     1381 ccgccaacgg cggctcgggc cgcaggtacg cgccgacgag gcccgcgtcg agctcgccgc
     1441 gcccggcgtc gagcagggcc agtcccgcag cggaccatcc ccgcactccc gcgtgcgcga
     1501 cgacgtcgcc cggccgcgcg ccggaccgga gcacagggtc acgacccgcc aggtcgccgt
     1561 gcaccgtgac ggcgacgacc gcgaccggcc ccgaggacag gtcgccaccg acgaccccga
     1621 caccgagggg cgcgcacacg gccgcgaggc cccgcgcgag gccttcgacc cacgacgccg
     1681 gaaggtccgc gggcgcgacg agcgcgacca cgagcgcagt cggcctcgca cccatcgccg
     1741 cgatgtccgc gaggttctga gcggccgcac gacggcccac gtcctcaccg gtggaccagc
     1801 gccggcggaa gtgccggtcc tcgacgagca cgtcggtcga gacgaccacg cgaccgtccg
     1861 gcgcggcgac gaccgcggcg tcgtcccccg gcgggacgag cgtggcgtcg ccgagcggca
     1921 gctgcgggaa gatcagggcg agcagcgcgt cctcggacag gtcggcgacg gtcggctcgt
     1981 caggggaact cacgcccgcc accgtatccc gtcggcgtcc tgccgccggt cggccgccgt
     2041 ggccggcatc agccgccgtg ggacgcgtta gcgtgacggg gtgaccgacc tgccgacccg
     2101 ctccgccggg acacccgggc acgtcggaca cgatcggcgg acccccgtcg ccagtccgcg
     2161 gcacgcgcgg cgcctgcctg ccgctgcggg cctcgtcctg gccctcgccg cgtgcgcacc
     2221 cggggtctcg gtcgccgagg ccccgcacgg cgccgacccg gtctgtgcga gcgtcgttct
     2281 cgcgcttccc gaccgcctcg gcgacctgcc gcgacagcgc acgacgagcc aggccagcgc
     2341 cgcctggggc accgcagccg cgcccgtggt gctccggtgc ggcgtcgagg tcccgggccc
     2401 gacgacggtg cactgcgtga gcgtggagac gcccgacggc ccgagcatcg actggctcgc
     2461 tgtcgctgac gaccctgcag cgcagggcac cacagactgg acgttcacga cctacggccg
     2521 cgacccggca gtcgaggtcc gcgtacccgg gtcggtcgcg agcacgcgct cgacgtcgtt
     2581 cctcgaccag ctcggtccgg ccatcgcgca ggtcgagccg acgaggtcgt gcctctgagc
     2641 ctcgcccgcc gggggccgag gcctcagcgc aggccggtgg ggcggtgcag cgcgagctgc
     2701 agcagctcgt cgatcaggtc cgggtaggag aggccggtcg cctgccacat ccgcgggtac
     2761 atcgagaacg gcgtgaaccc cggcatggtg t
//
LOCUS       DASRKH010000132         2728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_49945, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000132 DASRKH010000000
VERSION     DASRKH010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49945"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /gene="ruvC"
                     /locus_tag="VFC06_02240"
     CDS             complement(<1..524)
                     /gene="ruvC"
                     /locus_tag="VFC06_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017887356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HZL80751.1"
                     /translation="MRILGVDPGLTRCGVGVIEAGRPPRLVAVGVIRTEASQSADLRL
                     MTISLGIEEWLDTHHPDAVAIERAFAQHNVPTVMGVAQVAGLAMVAAARRGIPVGLHT
                     PSEVKAAVTGYGNAPKAQVQHMVARILGLAEAPKPADAADALALAICHSWRQGSPTAP
                     GYTDQTDAQRAWADA"
     gene            complement(524..1285)
                     /locus_tag="VFC06_02245"
     CDS             complement(524..1285)
                     /locus_tag="VFC06_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005868502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZL80752.1"
                     /translation="MSGHSKWATTKHKKAAIDAKRGKLFARLIKNIEVAARTGGGDPS
                     GNPTLFDAILKAKKNSVPADNIDRAVKRGSGQEAGGADYQNIMYEGYGPGGVAVLVEC
                     LTDNRNRAASDVRVAFTRNGGSMADPGSVSYLFSRRGVVVVSKTPGLTEDDVLMAVLD
                     AGADEVEDIGDSFEIMSEATDVVAVRTALQEAGLDYDSAEVQFVPATEVEVDIDGARK
                     IMKLIDALDDSDDVQNVYANFNASDEVMAALDDED"
     gene            complement(1441..2034)
                     /gene="pdxT"
                     /locus_tag="VFC06_02250"
     CDS             complement(1441..2034)
                     /gene="pdxT"
                     /locus_tag="VFC06_02250"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HZL80753.1"
                     /translation="MTTIGVLALQGDVREHVVSLEAVGARAVPVRRLSELEHVDGLVL
                     PGGESTTIDKLLRAFGLFEPLRARIAGGLPVYGSCAGMILLADRVLGAGPDQRTLGGI
                     DMTVRRNAFGRQVDSFEADVDVAGLAGGPVHAVFIRAPWVEEVGEDVEVLASVSLPGG
                     AATHIVAVRQGVLLATSFHPEMDADHRLHELFVGMVG"
     gene            complement(2031..2561)
                     /locus_tag="VFC06_02255"
     CDS             complement(2031..2561)
                     /locus_tag="VFC06_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HZL80754.1"
                     /translation="MTYFSDEWVRGADGMLHRRGARVMVFDGAGRVLLQRGHDMDEPA
                     RHWWFTPGGGIHRGEDPRAAAARELAEETGIVVAALDLEGPVAERSAIFDFAREWVHQ
                     DEVFFTARIAGADLDSSGWTEVERQFLVEQRWVPVEEIGALDGELFPEELPAFAAALL
                     AGWDGAVRQLRREDTR"
     gene            complement(2558..>2728)
                     /locus_tag="VFC06_02260"
     CDS             complement(2558..>2728)
                     /locus_tag="VFC06_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005565691.1"
                     /note="with PdxT forms pyridoxal 5'-phosphate from
                     glutamine, either ribose 5-phosphate or ribulose
                     5-phosphate, and either glyceraldehyde 3-phosphate or
                     dihydroxyacetone phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HZL80755.1"
                     /translation="FKSGAPARRAAAIVKATTFFDDPTVIAEVSRGLGEAMVGINVDD
                     IPTSHRLAERGW"
BASE COUNT          397 a          983 c          953 g          395 t
ORIGIN      
        1 gcgtcggccc acgcccgctg cgcgtcggtc tggtcggtgt acccgggcgc cgtcgggctg
       61 ccctggcgcc atgagtggca gatcgccagt gccagcgcgt cggccgcgtc ggccggcttc
      121 ggcgcctcgg cgagcccgag gatgcgggcg accatgtgct gcacctgcgc cttgggggcg
      181 ttgccgtagc ccgtcacggc cgccttcacc tccgacggcg tgtggaggcc aacggggata
      241 ccgcggcgcg cggccgccac catggccagc cccgcgacct gcgccacccc catgacggtg
      301 ggcacgttgt gctgggcgaa cgcgcgctcg atggcgacgg cgtcggggtg gtgggtgtcc
      361 agccactcct cgatgccgag ggagatggtc atgaggcgca ggtccgcgga ctgggaggcc
      421 tccgtgcgga tcacgccgac ggccaccagc cgcggcgggc gccccgcctc aatgacaccg
      481 acaccgcatc gggttagccc cggatcgacg ccgaggatgc gcatcagtcc tcgtcgtcga
      541 gcgcggccat gacctcgtcg gaggcgttga agttggcgta cacgttctgg acgtcgtcgg
      601 agtcgtcgag ggcgtcgatc agcttcatga tcttccgggc gccgtcgatg tccacctcca
      661 cctcggtggc agggacgaac tgcacctcgg cggagtcgta gtcgaggcct gcctcctgca
      721 gggcggtgcg gacggcgacg acgtccgtgg cctcggacat gatctcgaac gagtcaccga
      781 tgtcctccac ctcgtcggca ccggcgtcca ggacggccat gagcacgtcg tcctccgtga
      841 gacccggggt cttggagacc acgacgacgc cgcggcggga gaacaggtag gagacggacc
      901 ctgggtcggc catgctgccc ccgttgcggg tgaaggccac tcggacgtcg gaggcggccc
      961 ggttgcggtt gtcggtcagg cactcgacga ggacggccac gccaccgggg ccgtagccct
     1021 cgtacatgat gttctggtag tccgctccgc cggcctcctg gccggacccg cgcttgaccg
     1081 cgcggtcgat attgtcagcc gggaccgagt tcttcttggc cttcaggatg gcgtcaaaaa
     1141 gggtggggtt gccggagggg tcacccccac cggtacgggc cgccacctcg atgttcttga
     1201 tgaggcgggc gaagagcttg ccgcgcttgg cgtcgatcgc ggccttcttg tgcttggtgg
     1261 tggcccattt ggaatgaccg gacaacgcgt tctccttccc gacctggcgg caccggcgcg
     1321 cgcagatcgg ctctgggttt cacaacgggg cgcgagcccc acgggctgcc tcgattctac
     1381 ccgcagccac cgccgcccac ggtcaggccc accgtgacgc cgacgcaggg tggcggtgac
     1441 tcagccgacc atcccgacga agagctcatg caggcggtgg tccgcatcca tctccgggtg
     1501 gaaggacgtc gcgaggagca caccctggcg cacggcgacg atgtgggtgg ccgcgccacc
     1561 gggcaggctc acgctggcga gcacctcgac gtcctccccc acctcctcca cccacggggc
     1621 gcggatgaag acggcgtgaa cgggcccgcc cgcgagcccc gccacgtcga cgtcggcctc
     1681 gaacgagtcc acctgccgcc cgaaggcatt gcgccgcacg gtcatgtcga tgcccccgag
     1741 ggtgcgctgg tcgggcccgg caccgaggac gcggtcggcg aggaggatca tgccggcgca
     1801 cgagccgtac acgggcagcc ccccggcgat ccgcgcccgc aacggctcga agagcccgaa
     1861 cgcccgcagg agcttgtcga tggtggtcga ctcgcccccc ggcagtacca gtccgtccac
     1921 gtgctcgagc tcggacagcc ggcgcaccgg aaccgcccgt gcacccacgg cctcgaggct
     1981 aacgacgtgc tcgcgcacgt cgccctggag ggcgaggacg ccgatcgtcg tcatcgggtg
     2041 tcctcacgcc gcaactggcg tacggcgccg tcccagccgg ccagcagggc ggccgcgaac
     2101 gcgggaagct cctccgggaa gagttcgccg tcgagggcgc cgatctcctc gaccggcacc
     2161 cagcgctgct ccaccaggaa ctgccgttcg acctcggtcc atccactcga gtcgaggtcg
     2221 gcaccggcga tccgggcggt gaagaacacc tcgtcctggt gcacccactc gcgcgcgaag
     2281 tcgaagatgg cgctcctctc cgccaccggg ccctcgaggt ccagggctgc cacgacgatg
     2341 ccggtctcct cggcaagttc ccgggccgct gccgcgcgcg gatcctcccc ccggtggatg
     2401 cccccacccg gggtgaacca ccagtggcga gccggctcgt ccatgtcgtg gccgcgctgg
     2461 aggaggaccc tacccgcccc gtcgaagacc atgacgcgag cgccgcggcg atggagcatg
     2521 ccgtcggcgc cgcgcaccca ctcgtcggag aagtaggtca ccacccgcgc tcggcaagtc
     2581 ggtgggaagt ggggatgtcg tccacgttga tgcccaccat cgcctccccg aggccacgcg
     2641 agacctcggc gatgacggtg gggtcgtcga agaacgtcgt cgccttcacg atcgcggccg
     2701 cacgcctggc gggggcgccc gacttgaa
//
LOCUS       DASRKH010000133         2510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_18988, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000133 DASRKH010000000
VERSION     DASRKH010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18988"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..1384
                     /locus_tag="VFC06_02265"
     CDS             167..1384
                     /locus_tag="VFC06_02265"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010155029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HZL80756.1"
                     /translation="MAKIKVQGKVVELDGDEMTRIIWQFIKERLIHPYLDIDLEYYDL
                     SIENRDATDDQVTHDAAHAIKKHGVGVKCATITPDEARVAEFGLKKMWVSPNGTIRDI
                     LGGVVFREPIIISNIPRLVPGWTKPIVIGRHAHGDQYKATNFKVPGAGQLTITFTPAD
                     GSEPIEHVVANYGADGGVAMGMYNFNKSIEDFARATFAYGLQRGYPVYLSTKNTILKQ
                     YDGAFKDIFQDVFDRDFKAQFDVQGLTYEHRLIDDMVASAMKWEGGYVWACKNYDGDV
                     QSDTVAQGFGSLGLMTSVLMTPDGLTVEAEAAHGTVTRHYRQHQEGKPTSTNPIASIF
                     AWTGGLKHRGKLDGTPEVTGFAEALEDVVIKTVESGKMTKDLAALVGPDQEWLTTEGF
                     LEALDENLAARLG"
     gene            1433..2419
                     /locus_tag="VFC06_02270"
     CDS             1433..2419
                     /locus_tag="VFC06_02270"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HZL80757.1"
                     /translation="MTVPVNVTVTGAAGQIGYALLFRVASGQLLGPDVPVRLRLLEIP
                     QAVRAAEGTAMELEDCAFPLLAGIDIFDDAKAAFDGVNVGLLVGARPRGAGMERGDLL
                     AANGGIFGPQGEAINAGAADDVRILVVGNPANTNALIASAHASDVPAERFNAMMRLDH
                     NRALAQLAVKTGVPVRDIDNVIIWGNHSAKQYPDIAHAVVDGKRATDIVNDDAWVRDT
                     FVPTVAKRGAAIIEARGASSAASAANAAMEHVNNWVNGTPEGVWTSVAMPSDGSYGVP
                     EGLVCGFPAVSKNGAWEIVQGLEIDAHAREMIDVSVAELIEERDAVKELGLL"
BASE COUNT          461 a          823 c          814 g          412 t
ORIGIN      
        1 cctgcgttgt ctcgtcttcc atgtctctcc tcgtgtatcc gcaaacgaat gctctcgaac
       61 gcatcctggc atgcgcatcg gcaaacgtcg tgggggacgc tcgggtgtcg tgcgtggact
      121 agcctggtcc aggtaccaaa gccgcgttgt tgaggagagt cgccgcatgg caaagatcaa
      181 ggttcagggc aaagtcgtcg aactcgatgg cgacgagatg acaaggatta tctggcagtt
      241 catcaaggag cgcctgattc atccgtatct ggacatcgac cttgagtact acgacctcag
      301 catcgagaat cgtgacgcca cggacgatca ggtcacgcac gatgctgcgc acgccatcaa
      361 gaagcacggc gtgggcgtca agtgcgccac gattacgccg gacgaggcgc gcgtcgcgga
      421 gttcggtctc aagaagatgt gggtgagccc caacggcacg atccgggaca tcctgggcgg
      481 cgtggtgttt cgcgagccca tcatcatctc caacattcct cggttggttc cgggctggac
      541 caagccgatc gtcatcggcc gtcacgcgca cggcgaccag tacaaggcca ccaacttcaa
      601 ggtgccgggt gcgggccagc tcaccatcac cttcaccccc gcggacggct cggagccgat
      661 cgagcatgtg gtggccaact acggcgccga cggcggtgtg gccatgggca tgtacaactt
      721 caacaagtcc atcgaggact tcgcgcgtgc cacgtttgcc tacggcctgc agcgcggcta
      781 cccggtctac ctgtccacca agaacacgat cctcaagcag tacgacggcg cgttcaagga
      841 catcttccag gacgtgttcg accgcgactt caaggcccag ttcgacgtgc agggcctcac
      901 gtacgagcac cgcctcatcg acgacatggt cgcctcggcc atgaagtggg agggcggcta
      961 cgtctgggca tgcaagaact acgacggcga tgtccagtcg gacaccgtgg cgcagggctt
     1021 cggctcgctg ggcctcatga cgtccgtgct gatgacgccc gacggtctga cggtggaggc
     1081 tgaggccgcg cacggcaccg tcacgcgcca ctaccgccag caccaggagg gcaagcccac
     1141 ctcgaccaac ccgatcgcgt cgatctttgc gtggacgggt ggcctcaagc accgcggcaa
     1201 gttggacggc acccccgagg tgaccggctt cgccgaggcc ctcgaggacg tcgtcatcaa
     1261 gaccgtagag tccggcaaga tgaccaagga tctcgctgcg ctggtcggcc ccgaccaaga
     1321 gtggctcacc accgaaggct tcctcgaggc gctcgacgag aacctcgccg cccgcctcgg
     1381 ctaactaccc accaccagcc cagggcatcg tcgcccaagg aaggttacga ccatgactgt
     1441 ccccgtcaat gtcactgtca ccggagccgc cggccagatc ggctacgcgc tcctcttccg
     1501 ggtggcgtcc ggccaactgc tggggcccga cgtcccggtg aggctgcgcc tactggagat
     1561 tccgcaggcg gtgcgtgccg ccgagggcac ggccatggaa ctcgaggact gcgccttccc
     1621 cctgctcgca ggcatcgaca tttttgacga tgcgaaggcg gcgttcgacg gcgtcaacgt
     1681 ggggctcctg gtgggagcac gcccgcgcgg tgcagggatg gagcgcggcg acctgctcgc
     1741 ggccaacggc ggcattttcg gtccccaggg ggaggcgata aacgccggcg ccgcggacga
     1801 cgtgcgcatc ctcgtggtcg gcaaccccgc gaacaccaac gctctcatcg cgtccgccca
     1861 cgcgtccgac gtccctgccg aacgcttcaa cgcgatgatg cgactcgatc acaatcgcgc
     1921 tctcgcgcag ttggccgtca agactggcgt cccggtgcgg gacatcgaca acgtcatcat
     1981 ctggggcaac cactccgcca agcagtaccc cgacattgcc cacgcggtgg tggacggcaa
     2041 gcgcgcgacc gacatcgtca acgacgacgc gtgggtgcgt gacactttcg tgccgaccgt
     2101 ggccaagcgt ggtgcggcga tcatcgaggc ccgcggcgcg tcgtcggcgg cctctgccgc
     2161 gaacgcggcg atggagcacg tcaacaactg ggtgaacggc actcccgagg gtgtgtggac
     2221 gtcggtggcg atgccgtccg acggctccta cggcgtgccc gagggccttg tgtgcggatt
     2281 cccggcggtg tcgaagaacg gcgcgtggga gatcgttcag ggcctcgaga tcgatgcaca
     2341 cgcccgcgag atgatcgatg tctcggtcgc cgagctcatc gaggagcgcg acgcggtcaa
     2401 ggagttgggg ctgctctagc ggcccaccgc acgatccaag aaagggccgt cgcttgcgcg
     2461 acggcccttt cttgatggtg gaacggcgac gacggaccgg aatgcggcgt
//
LOCUS       DASRKH010000134         3196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_27805, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000134 DASRKH010000000
VERSION     DASRKH010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27805"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..651)
                     /gene="rplI"
                     /locus_tag="VFC06_02275"
     CDS             complement(205..651)
                     /gene="rplI"
                     /locus_tag="VFC06_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HZL80758.1"
                     /translation="MAKLILTHEVSGLGTAGDVVEVKDGYATNFLVPRKLATPWSAGA
                     EKSIDSMRQARRGRELASVEDAQAARDSLQANPVTVEANAGPSGRLFGAITTAEIADA
                     IGTRALVDKRRIQIGQPIKALGDYQVKVSLHDGVVATVDVKVVAAS"
     gene            complement(663..899)
                     /gene="rpsR"
                     /locus_tag="VFC06_02280"
     CDS             complement(663..899)
                     /gene="rpsR"
                     /locus_tag="VFC06_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HZL80759.1"
                     /translation="MAKIDTRKPRKKVNPLKAAKVKTIDYKDTALLRKFISDRGKIRS
                     RRVTGVTVQEQRQIARAIKVARELALLPYAGSGR"
     gene            complement(946..1437)
                     /locus_tag="VFC06_02285"
     CDS             complement(946..1437)
                     /locus_tag="VFC06_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015884655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HZL80760.1"
                     /translation="MAGDTQITVVGNLTGDPELRFIQSGAAVANFTVASTPRTFDRQS
                     NDWKDGDTLFMRCSLWREAAENVAESLTKGMRVVVTGRLVSRSWEANGEKRTVMEMQV
                     DEVGPSLRYATAKVTRTPRTGGQGGGGFSGGGQGGGSANTPAGGAANDPWASAPSSDE
                     PPF"
     gene            complement(1472..1762)
                     /gene="rpsF"
                     /locus_tag="VFC06_02290"
     CDS             complement(1472..1762)
                     /gene="rpsF"
                     /locus_tag="VFC06_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HZL80761.1"
                     /translation="MRKYEMMVILDPEVDERTVKPSLEKYLAVVTNDKGTVDSLDVWG
                     RRRLAFPIKKKSDGVYAVINFTAESTTAKELERQLGLNETILRLKLLRPDAH"
     gene            1980..>3196
                     /locus_tag="VFC06_02295"
     CDS             1980..>3196
                     /locus_tag="VFC06_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate--amine ligase"
                     /protein_id="HZL80762.1"
                     /translation="MKRVLVVGIGGDIGVYAFQRAAHEKYGWQPVVISQVTTTFMKHS
                     RLVKGIIEPDVHTPDALIARLNDFAADHPDDELILFTNLDWHVRVLAQRRAEIDSRWL
                     MPLCDLATFDRVSSKVAFAAACDAVGVRTPRTLSVVFAPQAAPADAAGSDVVRAEEAI
                     AKIGDLGLEYPLIGKPSASADWFKAAFPGKLKIHHFANESELVEVLSHLEELAYPSEF
                     LVQEFVPGDETHMRSLTAYRDRQGSVTLLAGGQVLLEEHTPGTLGIPAAILTGVDEEA
                     FESARRILDATDYYGFANFDYKVSSRDGSRVFFEVNPRIGRNNFYVTAAGVNVADAVA
                     RDLLPDHYAAHAGLDGDPTAVPSGEILYSVMPYRVLLRYLRDPELRSRVRAAKKRSGM
                     FHPLEYRGDASLTR"
BASE COUNT          538 a         1048 c         1033 g          577 t
ORIGIN      
        1 gtggatagaa atctagtaac atcagttgac ggatatctac caggcgtgtt aggggcacac
       61 agcatgggga aaactccggc cgtaaatcat cggcgtgtcg cctctcggcg tgtcgcggcc
      121 gtccgcgccg ggcacgacaa agccccggga ttcctccctc gccaaggcga gatgaggggc
      181 attcccgggg cgtgtcgaat cgccctaaga ggcggcaacc accttgacgt ctaccgtcgc
      241 gacgacgccg tcgtgcagcg acaccttgac ctggtaatcg cccagcgcct tgatcggctg
      301 accgatctga atgcggcgct tgtcgaccaa ggcgcgggtg ccgatggcgt ccgcaatctc
      361 ggccgtcgtg atcgcgccga agaggcgacc ggacgggccg gcgttcgcct cgacggtcac
      421 cgggttggcc tgcagtgaat cgcgggccgc ctgggcgtct tcgacgcttg cgagctcgcg
      481 accgcggcgc gcctggcgca tcgagtcgat tgacttctcg gcacccgcgg accacggagt
      541 ggccagcttg cggggaacca ggaagttggt ggcgtagccg tccttgacct cgacgacatc
      601 gcccgcagtg ccgaggccgg agacctcgtg cgtgagaatg agttttgcca tgtcagttcc
      661 ccttaacggc cggagccggc gtaggggagc agtgcgagtt cgcgcgcaac cttgatggcg
      721 cgggcgatct ggcgctgttc ctgaacggtg acacccgtga cgcggcgcga acggatcttt
      781 ccgcggtccg agatgaactt gcgcagcagc gcggtgtcct tgtagtcgat cgtcttgacc
      841 ttggcggcct tgagcgggtt gacctttttg cgcggcttgc gcgtgtcaat ctttgccatg
      901 cttgtctttc cttacatcaa gaagtggggc gactcgtcgt cgcgtttaga acgggggctc
      961 gtccgaggac ggggccgatg cccacgggtc attggctgca ccgccggcgg gggtgttggc
     1021 tgagccgcca ccctgaccgc caccgctgaa gccgccgccg ccctgaccgc cggtacgcgg
     1081 agtgcgcgtg accttggccg tggcgtagcg gagggacggg ccgacctcgt cgacctgcat
     1141 ctccatgacg gtgcgctttt caccattggc ttcccacgag cgcgacacga gcctgccggt
     1201 cacaacgacc cgcatgccct tggtgagcga ctcggccaca ttctcagcgg cctcgcgcca
     1261 caaggagcag cgcatgaaca gggtgtctcc gtccttccag tcgttcgact gacggtcgaa
     1321 cgtgcgggga gtggacgcga ccgtgaagtt cgccacggcg gcccctgact gaatgaatcg
     1381 cagttcgggg tctccggtga ggtttcccac cacggtgatc tgagtgtcgc ctgccatgtc
     1441 ttatccctgt cgtctcgtgg agttgccggt gctagtgagc gtccggacgc agcagcttca
     1501 aacgcaggat ggtctcgttc agaccaagct gacgttcaag ctccttcgcc gtcgtggact
     1561 ccgcggtgaa gttgatgacc gcgtagacgc catcgctctt cttcttgatg gggaaggcca
     1621 aacggcgacg gccccataca tcgaggctgt cgactgtgcc cttgtcgttg gtaaccacgg
     1681 cgaggtactt ctcaagcgac ggcttgaccg tacgctcgtc gacctcgggg tcaaggatga
     1741 ccatcatttc gtacttgcgc ataaataacc cacctcctct ggactaaacg gctacggacg
     1801 ttccgtagca ggagggtgac gcatgctgtg acgaggctcc ccgattgcgg gaggcccgag
     1861 cgccacgagc gacaaggata ccgtgcgggg gagtcaggcg aaagtcggac tgtggaaaga
     1921 ctccctccgc gcctcgggaa cgtcactaga atgaggcgct acacgatgag gaggaacggg
     1981 tgaagcgagt cctggtcgtg ggcatcggcg gcgatatcgg cgtctatgcc ttccaacgcg
     2041 ccgcgcatga gaagtacggg tggcagcccg tggtcatctc ccaggtgacc acgacgttca
     2101 tgaagcattc gcggctggtc aagggcatca tcgagcccga cgtacacact cccgatgccc
     2161 tcatcgcccg cctcaacgac ttcgccgcag accaccccga cgacgagctc atcctcttca
     2221 cgaacctcga ttggcacgtc cgcgtgctgg cgcagcgacg ggccgagatc gactcccgat
     2281 ggctcatgcc gttgtgcgat ctcgccacct tcgaccgcgt gtcgtccaag gtcgccttcg
     2341 ccgccgcgtg cgacgcggtg ggagtgcgca cgccccgcac gctgtccgtg gtgttcgcac
     2401 cgcaagcggc gcccgccgac gccgccggtt ccgacgtcgt ccgggccgag gaagcgatcg
     2461 ccaagatcgg cgacctggga ctcgaatacc cgctcatcgg caagccgtcc gcgagcgccg
     2521 actggttcaa ggccgcgttc cccgggaagc tgaagatcca ccactttgcc aacgagtctg
     2581 aactggtgga ggtgctgagc catctggagg agctcgcgta cccgagcgag ttcctggtgc
     2641 aggagttcgt tcctggcgac gagacccaca tgcgctcgct caccgcgtac cgcgaccggc
     2701 agggaagcgt gacgctcctg gccggcgggc aggtgctgct tgaggagcac accccgggga
     2761 cgctcggcat tcccgccgcc attctcaccg gggtagacga ggaggccttc gaatccgcgc
     2821 gccgcattct cgacgccacc gactactacg gcttcgccaa cttcgactac aaggtcagca
     2881 gtcgtgacgg atcgcgcgtg ttcttcgaag tcaacccgcg catcggccgc aacaacttct
     2941 atgtcacggc ggcgggggtg aacgtcgccg acgcggtcgc cagggacctg ctccccgacc
     3001 actacgcggc ccacgcggga ctcgacggcg acccgactgc ggtgccgtcc ggcgagatcc
     3061 tgtactccgt gatgccgtat cgcgtcctgc tgcggtacct tcgtgacccc gagctccgct
     3121 cccgcgtccg cgccgccaag aagcgctcgg gcatgttcca cccgctggag tatcgcggcg
     3181 acgccagcct cacgcg
//
LOCUS       DASRKH010000135         2353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_21071, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000135 DASRKH010000000
VERSION     DASRKH010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21071"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1304)
                     /locus_tag="VFC06_02300"
     CDS             complement(<1..1304)
                     /locus_tag="VFC06_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HZL80763.1"
                     /translation="MTDFPMPEPGVDPSGSTTASGAPAVSDRNSLSLGSNGPLLLHDV
                     HLVDTLAHFNRENVPERRPHAKGAGAFGEFETTSDVSAYTRAALFQQGAKTRMLARFS
                     TVAGELGSPDTWRDVRGFALKFYTTAGNYDMVGNNTPVFFIRDPMKFPHFIRSQKRLP
                     DSGLRDNHMQWDFWTLNPESSHQVTYLMGDRGLPKSWRNMNGYSSHTYMWVNAAGERF
                     WVKYHFHTQQGIENLDGAEAERIAGADADFHRRDLYNAIKGGDFPKWKLSVQVMPYED
                     ARNYRFNPFDLTKVWPHSDYPLIEVGVMTLNENPKNYFAQIEQAAFAPANVVPGIGFS
                     PDKMLLGRVFAYADAHRARIGTNFDQLPVNRPIVPVNVYHADGHMNFLDTGDQAVYAP
                     NTAGRSYSDFEGPVPEGWEADGDMVRQAYKLHAEDDDFGQAGT"
     gene            1549..2187
                     /locus_tag="VFC06_02305"
     CDS             1549..2187
                     /locus_tag="VFC06_02305"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HZL80764.1"
                     /translation="MTVNDDEVPAADKLVVTMWGTYGAGMEEIAARVAELLGVALRQQ
                     AFSSEEIQAAQDTGGKDSAIWFLVGATGHNPRMFDRLLPGVKKEHERMAARMATEVLA
                     DAAQGGVIQGRTATVVLADWPKVLHVKLDGPVRDRIARAASKHNISLKDAADRQQWED
                     DVRSQMSIDLVDWSPLDIDLFDLVLNTIQLDTETCAQMIVAAAKVKAARLAG"
BASE COUNT          360 a          743 c          851 g          399 t
ORIGIN      
        1 gtgccggcct ggccgaagtc gtcgtcctcg gcgtgcagct tgtaggcctg gcgcaccatg
       61 tcgccgtcgg cctcccaacc ttcgggaacc ggcccctcga agtcggagta cgagcggccg
      121 gcggtgttgg gcgcgtacac cgcctggtcc ccggtgtcga ggaagttcat gtgcccgtcg
      181 gcgtggtaga cgttcacggg cacgatggga cggttcaccg ggagctggtc gaagttggtc
      241 ccgatgcgcg cgcggtgggc gtccgcgtag gcgaacacgc ggccgagcag catcttgtcc
      301 ggcgagaagc cgatgccggg aaccacgttc gcgggggcga aagctgcctg ctcgatctgc
      361 gcgaagtagt tcttcgggtt ctcgttcagg gtcatcacac ccacctcgat gagtgggtag
      421 tcggagtgcg gccacacctt cgtgaggtcg aacgggttga accggtagtt ccgggcgtcc
      481 tcgtacggca tcacctggac ggacagcttc cacttcggga agtcgccccc cttgatggcg
      541 ttgtagaggt cgcggcggtg gaagtccgcg tccgcgcctg cgatgcgctc cgcctcggca
      601 ccgtcgaggt tctcgatgcc ctgctgggtg tggaagtggt acttcaccca gaagcgctcg
      661 ccggcggcgt tcacccacat gtacgtgtgg gagctgtagc cgttcatgtt gcgccacgac
      721 ttcggcaggc cgcggtcacc catgaggtag gtgacctggt gggacgactc ggggttgagt
      781 gtccagaagt cccactgcat gtggttgtcc cggaggcccg agtcgggcag gcgcttctgg
      841 gagcggatga agtgcgggaa cttcatgggg tcccggatga agaataccgg ggtgttgttc
      901 ccgaccatgt cgtagttgcc cgcggtggtg tagaacttca gggcgaagcc gcgcacgtcg
      961 cgccaggtgt cgggggagcc gagctcgccg gccaccgtgg agaagcgtgc cagcatgcgc
     1021 gtcttcgcgc cctgctggaa cagcgccgcg cgggtgtagg ccgagacgtc cgacgtcgtc
     1081 tcgaactcgc cgaaggcgcc ggcgcccttg gcgtggggac ggcgctcggg gacgttctcg
     1141 cggttgaaat gggcgagggt gtccacgagg tggacgtcgt gcaacaggag tgggccgttc
     1201 gagcccagcg agagggagtt gcggtcactg acggcggggg cgccgctggc cgtggttgat
     1261 ccggacgggt ccacgcccgg ttccggcatc gggaagtcgg tcatcacatc tctccttggg
     1321 atggtcaatt cgatccacct cacgttaccc gcccgctccg cgaatcggca gggacaatgg
     1381 tcgccgattc ccgggcgccg gtatcccgct gtccgtcacc gcccgtcggc gggatcaccc
     1441 gaatgcgtcc accgatcggc tgacgagccc gaatgcgccc aactaggatg gcaatgtccc
     1501 catttctcgt ggtgatgtcg cgctgatggc gcggttgagg aggacgtcat gacggtgaac
     1561 gacgacgagg ttccggccgc cgacaagctg gttgtcacca tgtggggaac ctatggggcg
     1621 ggcatggagg agatcgcggc acgggtggcc gaattgctcg gggtcgccct ccgccagcag
     1681 gcgttctcca gcgaggagat ccaggccgcc caggacaccg gcggcaagga ttcagccatc
     1741 tggttcctcg tgggcgccac gggacacaac ccacgcatgt tcgaccgcct cctgccgggc
     1801 gtgaagaagg aacacgagag aatggccgcc cgcatggcca cggaggttct ggccgatgct
     1861 gcccagggcg gcgtgattca gggccgcacc gccacggtgg tcctggccga ctggccgaag
     1921 gtgctgcacg tgaagctcga cggacccgtg cgggaccgga tcgcgcgcgc cgcgagcaaa
     1981 cacaacatct ccctgaagga tgccgccgac cggcagcagt gggaggacga cgtccgctcc
     2041 cagatgtcga tcgacctagt cgactggagc cccctcgaca tcgacctttt cgacctggtc
     2101 ctcaacacga tccagctgga caccgagacc tgcgcccaga tgatcgtggc cgcggcgaag
     2161 gtgaaggcag cccggctcgc cgggtagcga ggggtggtcg aggccgtcgt ccgcgccggg
     2221 ccgggcctca cccgcgccac gcgcgcgggc gtgacggccg ggttcttggc ctcggtgctg
     2281 gtcgcggcga cgtcgtggtc ctccgccgcc gtccccgcgg tccgtgccga gccgctgcgg
     2341 ccgggctggg agc
//
LOCUS       DASRKH010000136         2153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_45732, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000136 DASRKH010000000
VERSION     DASRKH010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45732"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1473)
                     /locus_tag="VFC06_02310"
     CDS             complement(<1..1473)
                     /locus_tag="VFC06_02310"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HZL80765.1"
                     /translation="MAIFTANDFTSVGARQYRVRSGDIVPLARGLWTDEVRAAPTQVV
                     AENWIEIVGKMLPGAVISDRSAFAMRPVDGALFVSHRRTSPLELPGLTVYPDGKADDR
                     RDGDIPINRQGNLYASSPARALIDNAETRGRPGAVPRRLTREELHEQIVKIVSTYTPR
                     QVENLLAAVDGDANKTAAETVRVFVDAARGRINTVRSSSRAMNAAQRGEAFDHERVAL
                     FRQVIADLQLMAPVQRFVTEQRRAQMVPFYEAYFSNYIEGSTLTIDEAEQVVFHNADV
                     GKPEDAHDIAATYEIVADHAEMSRRPSDADQFMDSLRDRHRVMMAAHPTRQPGQWKTQ
                     RNQAGATVFVYPGHVPGTLRAGWEEGAVLTDPFQRAVYMMFMVSEVHPFADGNGRSAR
                     VAMNAELVPANLHRIIIPTVLRSDYMSGLLRTTAGNGPDGLHRVLSFAQSWVARGQFA
                     DLEIADSYLRATHALVDAGHAEREGIHLRLLKTGELEEWGD"
     gene            1765..>2153
                     /locus_tag="VFC06_02315"
     CDS             1765..>2153
                     /locus_tag="VFC06_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017792456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HZL80766.1"
                     /translation="MHEIVCPHCGKAFTIDEAGYADIVSQVRTKEFDAALHERLELAE
                     KDKQNAVELAKAQLESELQRAAAAKDSEIQALKAKLEADDVAKQLAVRDAVAVVEKQR
                     DELKGGLERAQLEMKLAETSLKDKYET"
BASE COUNT          402 a          691 c          696 g          364 t
ORIGIN      
        1 gtccccccac tcctcgagtt cgccggtctt cagcagacgg aggtggatgc cctcgcgttc
       61 ggcgtgaccc gcgtcgacaa gcgcatgggt cgcgcgcaag taactgtctg cgatctcgag
      121 atccgcgaac tggccgcggg cgacccagct ttgggcgaag ctcaacacgc ggtggagacc
      181 atcgggtccg ttcccggcgg tagtccgcaa aagccccgac atgtagtcgc tacgcagcac
      241 ggtcgggatg atgatgcggt ggaggttggc gggaaccaac tcagcgttca tcgccacgcg
      301 cgcagagcgg ccgttcccgt ccgcgaacgg gtgcacctcg ctgaccatga acatcatgta
      361 cacggcgcgc tggaacgggt cggtgagcac cgcaccttcc tcccagcctg ccctgagcgt
      421 gcctggcacg tggcccggat acacgaacac cgtggcgccc gcttggttgc gctgggtctt
      481 ccactggccg ggttgcctgg tcgggtgggc cgccatcatc acacggtggc gatcgcgaag
      541 agagtccatg aactggtcag cgtcgcttgg gcgccgtgac atctctgcgt ggtcggcgac
      601 gatctcgtag gtggccgcga tgtcgtgcgc gtcctcgggt ttgccaacgt cggcgttgtg
      661 gaacacgacc tgctccgcct cgtcgatcgt cagggtcgac ccctcgatgt agttcgagaa
      721 gtacgcctca tagaaaggca ccatctgggc gcgacgctgt tctgtgacga agcgctgcac
      781 cggcgccatg agctgcaggt cggcaatgac ctgccggaac agggcgacgc gctcgtggtc
      841 gaaggcctcg ccgcgctggg ctgcgttcat tgcacgcgaa ctggaacgca cagtgttgat
      901 gcgtcctcgg gcggcatcga caaagacgcg cacagtctcg gctgccgtct tgtttgcgtc
      961 gccatcgacg gcagcaagaa ggttctccac ctgccgcggc gtgtaggtgc tgacgatctt
     1021 gacgatctgt tcgtgtagtt cctcacgagt caggcgacgc ggcacggcgc caggcctgcc
     1081 tcgagtctcg gcattgtcga tgagcgcgcg cgccgggctt gacgcataga gattgccctg
     1141 ccggttgatc gggatgtcgc catcacggcg atcgtcggcc ttgccatctg ggtagaccgt
     1201 gagccccggc agctcgagcg gactggtgcg gcgatggctt acgaacagcg caccgtcgac
     1261 gggccgcatc gcgaatgcag acctgtctga aatgacagcg ccgggcagca tcttgccgac
     1321 gatttcgatc cagttctctg ccacaacctg agtaggggct gcgcgaacct catcggtcca
     1381 caggccgcgg gcaagcggaa cgatgtcacc agagcggact cggtactgtc gagcaccgac
     1441 cgacgtgaag tcgttcgccg tgaagatcgc catctcgctc tccgatcact tccctcgcta
     1501 aattgcgcct catgccagcg attcaagtat ttttctgcct aaatgatacc gctagtcggg
     1561 tagaaagtgc cgaaatagcg gcccgggcaa gcagggatcg ccacaatact tcagcgcgcg
     1621 agcgcgtcga tcccgtgtgg gtgctgcgaa gcccgacatc ggcgcggccc acgaggccga
     1681 tttccacagg cccagcgtcg gcgagtgccc ggcccgagcg tcggccgcac cccttaaact
     1741 gcgaccaaag aggggaccca caccatgcat gagatcgtct gcccgcactg cggcaaagcc
     1801 ttcactatcg acgaggcggg ctatgccgac atcgtctccc aggtgcgcac caaggagttc
     1861 gacgccgcac tccacgagcg gctcgaactg gccgagaaag acaagcagaa cgccgtcgag
     1921 ctggccaagg ctcagctgga aagcgagctt cagagggccg ccgccgccaa ggactccgag
     1981 atccaggcgc tcaaggccaa gctcgaggcc gacgacgtgg ccaagcaact cgccgtgaga
     2041 gacgcggtcg ccgtcgtcga gaagcagcgc gacgagctca agggcgggct cgagcgcgcg
     2101 caactcgaga tgaagctcgc cgagacctcg ctgaaggaca agtacgagac gca
//
LOCUS       DASRKH010000137         2069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_18659, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000137 DASRKH010000000
VERSION     DASRKH010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18659"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VFC06_02320"
     CDS             <1..658
                     /locus_tag="VFC06_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HZL80767.1"
                     /translation="QDLADLATVEESRVARLQGQVGTLRAEVDQLTEEQARLTGLQIG
                     SPGVGYDVASGKVAVTGPGITVRMTDAPADSQNLPDVRPDDLVVHQQDLQAVVNALWA
                     GGAEAMSLMDQRVISTSAFQCIGNVLSLHGRRYSPPYVVRAVGDPQKLRAALAVSPAV
                     QIYLEWVDAVGLGWQVTTAQDPLALPAYEGIAELKYAQVPRGVEVLPGLDAATSGSGS
                     "
     gene            655..1458
                     /locus_tag="VFC06_02325"
     CDS             655..1458
                     /locus_tag="VFC06_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HZL80768.1"
                     /translation="MTPPARVGLPRHGRRAPRTSAGSVALAGLGVVGELLITAGVLLL
                     AFLVWQLWWTDVEGNRAQAEIVRNLDWSQAPTAAPSAAPTPGATAGPVIAAPRRDEEP
                     PVEAEPDLRTTFATLQVPRWDGEPERPVSEGVDKATVLDVAGIGHYPGTAMPGGIGNF
                     AMAGHRTTYAKPFNRVAELQVGDPIVVRTESTWYVYRVTSTEIVRPQDVAVIAPVPGK
                     PGVAPTERSITVTTCHPMFSARQRFIVHGVLDYWAPVADGVPTEIAGGA"
     gene            1458..1616
                     /locus_tag="VFC06_02330"
     CDS             1458..1616
                     /locus_tag="VFC06_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80769.1"
                     /translation="MYGWLWRHLPGPTPVRVVILVVVAAAVLAACFTWLFPAIAPLMP
                     FNDTTVGV"
     gene            1618..>2069
                     /locus_tag="VFC06_02335"
     CDS             1618..>2069
                     /locus_tag="VFC06_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865063.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HZL80770.1"
                     /translation="MTRILVVDNYDSFVYTIVGYLAQLGAETVVVRNDAVPPVEERTG
                     FDGVLISPGPGTPAEAGASLQVIRDCAAAGIPMLGVCLGHQALGEVFGGTVTHAPELM
                     HGKTSQVFHSGVGVLAGLPSPFTATRYHSLAVVDGTFSDELEVTATVE"
BASE COUNT          278 a          755 c          731 g          305 t
ORIGIN      
        1 ccaggacctc gccgacctcg caaccgtcga ggagtcccgt gtcgcgcggc tgcaggggca
       61 ggtgggcacg ctgcgcgccg aggtcgacca gttgacagag gagcaggcgc gcctcaccgg
      121 cctgcagatc ggctcgcccg gggtcgggta cgacgtcgcc tccggcaagg tggccgtcac
      181 tggccccggg atcaccgtca ggatgaccga cgcgcccgcc gactcgcaga acctcccgga
      241 tgtgcgcccg gacgacctcg tcgtccacca gcaggacctc caggccgtgg tcaacgcgct
      301 ctgggcgggc ggcgccgagg ccatgagcct catggaccag cgggtgatct ccacgagcgc
      361 cttccagtgc atcgggaacg tcctgtcgct gcacggccgc aggtactctc ccccgtacgt
      421 cgtccgcgcg gtcggcgacc cgcagaagct gcgggcggcg ctcgccgtgt cgcccgccgt
      481 gcagatctac ctcgagtggg tcgacgcggt cggcctcggc tggcaggtca cgaccgctca
      541 ggacccgctc gccctgcccg cgtacgaggg gatcgctgag ctcaagtacg cgcaggtccc
      601 ccggggcgtc gaggtgctcc cgggcctcga tgcggcaacc tcgggttcag ggtcgtgacc
      661 ccgccagcgc gcgtcggcct tcctcggcac ggccgacgcg cgccgcggac gagtgcgggc
      721 tcggtagcgc tcgccggcct cggcgtcgtc ggcgagctgc tcatcacggc cggggtcctg
      781 ctgctcgcgt tcctcgtgtg gcagctgtgg tggaccgacg tcgagggcaa ccgcgcgcag
      841 gccgagatcg tccgcaacct cgactggtcg caggccccga ccgcggcgcc gtccgcggcg
      901 ccgacccctg gcgcgaccgc cgggccggtc atcgctgcgc cccgacgcga cgaggagccg
      961 cccgtcgagg cggaaccgga cctccgcacg acgttcgcga cgctgcaggt gccgcgctgg
     1021 gacggtgagc ccgagcgtcc cgtcagcgag ggcgtcgaca aggcgacggt gctcgacgtc
     1081 gccggcatcg gccactatcc cgggacggcg atgccgggtg ggatcggcaa cttcgccatg
     1141 gccggtcacc ggacgacgta cgccaaaccg ttcaaccgcg tcgcggagct gcaggtcggt
     1201 gacccgatcg tggtccggac cgagtcgacc tggtacgtgt accgcgtcac gtcgacggag
     1261 atcgtccgtc cgcaggacgt cgcggtcatc gctccggtcc ccgggaagcc tggggtcgcg
     1321 ccgaccgagc gatcgatcac ggtcaccacg tgccacccga tgttctcggc ccgtcagcgg
     1381 ttcatcgtgc acggcgtgct tgactactgg gcaccggtcg cggacggtgt ccccactgag
     1441 atcgccggag gagcctgatg tacggctggt tgtggcggca cctgcccggg ccgacgccag
     1501 tccgggtcgt gatcctcgtg gtcgtcgcgg cggcggtcct cgcggcatgc ttcacatggc
     1561 tgttcccggc gatcgcgccg ctcatgccgt tcaacgacac gactgtaggg gtgtgagatg
     1621 acccggatcc tggtggtcga caactacgac tcgttcgtct acacgatcgt ggggtacctc
     1681 gcgcagctcg gcgccgagac cgtcgtggtc cgcaacgacg ccgtgccccc cgtcgaggag
     1741 cgcacgggct tcgacggcgt cctgatctcc cccggaccgg gcacccccgc cgaggccggt
     1801 gcgagcctgc aggtcatccg cgactgtgcg gcagccggga tcccgatgct gggcgtgtgc
     1861 ctcgggcacc aggcgctcgg cgaggtgttc ggcgggaccg tcacgcacgc acccgagctc
     1921 atgcacggca agacgagcca ggtcttccac agcggtgtcg gcgtgctcgc cggtctcccg
     1981 agcccgttca ccgcgacgcg ctaccactcg ctcgcggtcg tcgacggcac gttctccgac
     2041 gagctcgagg tcaccgccac ggtcgagag
//
LOCUS       DASRKH010000138         3465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_16059, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000138 DASRKH010000000
VERSION     DASRKH010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16059"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="VFC06_02340"
     CDS             <1..883
                     /locus_tag="VFC06_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HZL80771.1"
                     /translation="IWPAELLAANGQGSKGGHVSDFGELQLPTEVVSSEFLTMESNKF
                     SSSRGHVIYVRDMLSRYQPDALRYFIAVAGPETSDADFTWAEFKRRTNDELVAGWGNL
                     VNRTATLLHKNIGSIPALVDMQDIDRAALDASEAGFDEVGRLIGAHRQRQAVSEAMRI
                     VGDANKYLADTEPWKLKTTDPERMKTVLGVAAQLVSDANTMLAPFMPHGAQKVHEALG
                     GTGIFAPMPRIAEVEDLDVADRSYPVIEGDYSVQHNTWHRKPLIAGTVVPEPSPIFTK
                     LDDDMVEAELERMRAEA"
     gene            880..1731
                     /locus_tag="VFC06_02345"
     CDS             880..1731
                     /locus_tag="VFC06_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HZL80772.1"
                     /translation="MSDVAWATPPEALPAPVVDNHCHLDFPYRGAVERSAAQILDEAG
                     RVGVTRAVQIGCDIDSARWTAEAIQADARLVGGVALHPNEAPLHAAGEHASGLDYDEA
                     LAEISRLARLPRMRVIGETGLDYHHTGPEGRDAQLRSFRDHIALAKELDLVLQIHDRD
                     AHADVLDVLERDGAPERTVFHCFSGDAAMAQVCAYRGYYVSFAGNITFKNAGSLRAAL
                     AVTPVENVLVETDAPFLTPHPQRGRVNSPSQVATTMRTIGEVLGCELEKACRTIDATS
                     ERLYGPW"
     gene            1921..2628
                     /locus_tag="VFC06_02350"
     CDS             1921..2628
                     /locus_tag="VFC06_02350"
                     /inference="COORDINATES: protein motif:HMM:NF019142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HZL80773.1"
                     /translation="MGTTVIVGAIAAATFTSAGAANIADTWLDRDRAVAEVPVAEISA
                     ASSEVEIQTVTTQNEIANGVVQQSDPHAKVGSQRVVQAGSPGVELVSYTVTTVNGVET
                     DRLPGISVLVTAPTDEIVAVGSLTIPAATDVQRGTNRALGQQMAAALYGWSGDQWSCL
                     DNLWARESGWSHTAGNQSSGAFGIPQALPGSKMAVYGADWQTNPATQIKWGLAYIQGR
                     YGTPCGAWGHFTAQNWY"
     gene            2635..>3465
                     /gene="rsmA"
                     /locus_tag="VFC06_02355"
     CDS             2635..>3465
                     /gene="rsmA"
                     /locus_tag="VFC06_02355"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HZL80774.1"
                     /translation="MAALLGPSEVRTLAASLGAAPTKKWGQNFVVDAGTVRRIVRIAG
                     VKPGDHVVEVGPGLGSLTLALLEAGARVTAIEIDPTLAEALPATVAARAPDDAERLTT
                     IQADAMTVTELETAPTLLVANLPYNVSVPVILRFLEFFPSLHTVLVMVQSEVADRLAA
                     PPGSRTYGIPSAKAAWYCKVRRAGNVGRSVFWPVPRVDSALVLMERREVPLTTATRLD
                     VFAVIDAAFAQRRKGLRGALASIAGSPTASEAALREAGIEPLTRGEQLDVAAFARIAE
                     A"
BASE COUNT          570 a         1156 c         1163 g          576 t
ORIGIN      
        1 gatctggccc gccgaattgc tggccgcaaa cggtcaggga tcgaagggcg gacacgtatc
       61 cgacttcggg gaactgcaat tgcccaccga agtggtgtcg agcgaattct tgacgatgga
      121 gtcgaacaag ttctcgtcgt cccgcgggca cgtcatctac gtgcgcgaca tgctttcccg
      181 gtatcagccc gatgcgctcc gctacttcat cgcggtcgcc ggtccggaga cctcggatgc
      241 cgacttcacg tgggccgagt tcaagcgccg caccaacgac gagttggtgg ccggctgggg
      301 aaacctggtc aaccgcaccg cgacgttgct ccacaagaac atcggctcga ttccggcgct
      361 cgtcgacatg caggacatcg accgcgcggc gctagacgcc tcggaagcgg ggttcgacga
      421 ggtgggcagg ctcatcggtg cccaccgcca gcgtcaggcc gtgagcgagg cgatgcgcat
      481 cgtcggcgat gccaacaagt acctggcgga caccgagccc tggaagctca agaccaccga
      541 ccctgagcgc atgaagacgg tactgggcgt cgcagcacag ctggtgagcg acgcgaacac
      601 gatgctcgcg ccgttcatgc cgcacggcgc acagaaggtg cacgaggcgc tcggcggcac
      661 cgggatcttc gccccgatgc cgcgcatcgc cgaggtggag gacctggacg tcgcggatcg
      721 cagttacccc gtgatcgagg gcgactactc ggtgcagcac aacacttggc atcggaaacc
      781 cctcattgca ggcaccgtgg tgcctgagcc gagcccgatc ttcaccaaac tcgacgacga
      841 catggtggaa gccgagctcg agcggatgcg cgccgaggcg tgagcgacgt cgcgtgggcg
      901 acgccgcccg aggcgcttcc cgcgcccgtg gtggacaacc attgccacct cgactttccc
      961 tatcggggtg cggtcgagcg ttccgcggcg cagattcttg acgaggcggg cagggtgggt
     1021 gtcacgcgag ccgtccagat cgggtgcgac atcgactccg cacggtggac ggccgaggcg
     1081 atccaagcgg acgcgcgcct ggtcgggggc gttgcgttgc accccaacga ggctcccctt
     1141 catgccgccg gcgagcatgc cagcggcctc gactacgacg aggcgctcgc cgagatctcg
     1201 cgactagcac gcctgccgcg catgcgggtg atcggcgaga ccgggctcga ctaccaccac
     1261 accgggcccg agggacgcga cgcgcagttg cgctccttca gggaccacat tgcgctcgcc
     1321 aaggaactgg acctggtgct ccagatccat gaccgcgacg cccacgcgga cgtgctcgac
     1381 gtcttggagc gcgacggcgc ccccgaacgg actgtctttc attgcttctc cggagacgcg
     1441 gcgatggcgc aagtatgtgc atatcgcggc tactacgtga gcttcgcggg caacatcacg
     1501 ttcaagaacg cgggctcgtt gcgcgccgcg cttgccgtca cgccggttga gaatgtgctt
     1561 gtcgagactg atgcgccctt cctgacgccg cacccgcagc gaggtcgggt gaattcgccg
     1621 tcgcaagtgg cgacgacgat gcgcaccatc ggcgaggtgc ttggctgcga gcttgagaag
     1681 gcgtgcagga caatcgacgc gacctcggag cggctctacg gtccctggtg agccttatac
     1741 cactctgtgc cgcgtttctc ttgaatttgc gccgacgtgt gtcgatgtga gagaaatggg
     1801 ggttgaccac cgcgtgccca acttagggaa cctatgacgt atcggacgct tccgcctgct
     1861 ccgggcagtc gccgtgagcg acgcttgcga acgagcgcgc gcctgcgtac ctatcgtcag
     1921 gtgggtacca cggtcatcgt cggcgcgatc gccgcggcca ctttcacctc cgcgggcgcc
     1981 gcaaacatcg ccgatacctg gctcgaccgc gaccgtgcgg tggccgaggt cccggtcgct
     2041 gagatcagtg cggccagctc cgaggttgag atccagaccg tgaccacgca gaatgagatc
     2101 gcgaacggcg tcgtccagca atctgacccc cacgccaagg tgggctctca gcgggtcgtc
     2161 caagccggtt cacccggcgt cgagctggtg agttacaccg tcaccacggt caacggcgtg
     2221 gagaccgacc ggcttccggg catttcggtg ctggtgacgg cccccaccga tgagatcgtg
     2281 gccgtcggct ccttgaccat cccggccgcg acagacgtcc aaaggggcac caaccgcgcg
     2341 ctgggtcagc agatggcggc cgctctgtac ggttggtcgg gcgatcagtg gtcgtgcctc
     2401 gacaacctgt gggcgcgcga gtctggctgg tctcacaccg ccggaaacca gtcgtctggc
     2461 gcctttggta tcccgcaagc cctcccgggc agcaaaatgg cggtctacgg cgccgattgg
     2521 cagaccaacc ccgccaccca gatcaagtgg ggcctcgcct acattcaggg ccgctacggc
     2581 actccttgtg gcgcgtgggg tcacttcacc gcccagaact ggtactagcg gtctgtggcc
     2641 gccctgctcg ggccctctga ggtccgcacc ctcgccgcat cgcttggagc ggcgcccacc
     2701 aagaagtggg gacagaactt cgtcgtcgat gccgggacgg tgcggcgcat cgtccgcatt
     2761 gccggcgtga agcccggtga ccacgtcgtc gaggtcggtc ctggattggg atcgctgacg
     2821 ctcgcgctcc ttgaggcggg ggcccgcgtc accgcgattg agatcgatcc cacgcttgcc
     2881 gaggcgttgc cggccaccgt ggccgcgcgg gcgcccgacg acgcggagcg gttgaccacg
     2941 atccaggcgg atgccatgac cgtgacggag ctcgagaccg caccgacgct gttggtggcc
     3001 aatcttccct acaacgtctc cgttcccgtc atcctgcgct ttctcgaatt ctttccgtcg
     3061 ctgcacacgg tgctggtgat ggtgcagtcc gaggttgccg accgcctggc cgcgcccccc
     3121 ggttcccgca cgtatggcat cccgagcgcc aaggccgcct ggtactgcaa ggtccgccgc
     3181 gcggggaacg tgggacggtc cgtgttttgg ccggtgccga gggtcgattc ggcgctggtg
     3241 ctgatggagc gcagggaggt gcccctcacc acggcgacgc ggctagacgt cttcgccgtc
     3301 attgacgcgg cgttcgctca gcgccgcaag gggctgcggg gggcactggc ctcgatcgcg
     3361 ggttcgccga cggcctccga ggccgcacta cgtgaggccg gcatcgagcc gctcacgcgg
     3421 ggagaacaac tggacgtggc cgccttcgcg cgcatcgccg aggcg
//
LOCUS       DASRKH010000139         3405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_31523, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000139 DASRKH010000000
VERSION     DASRKH010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31523"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1162)
                     /locus_tag="VFC06_02360"
     CDS             complement(<1..1162)
                     /locus_tag="VFC06_02360"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZL80775.1"
                     /translation="MTAAHPGTRTRALSLRARVLVGLAAIALVAVLAAAAVTLTTQSY
                     LVSQVDDRLLAFSGPSHGTGLGDADDYPVPSAAEEDGEGSIATPSTATTDWWNERPSD
                     AVRGVLTDTGTLVVQFQPNVYSDETFDAPLINPADLSRGSLTYITAASAGGHEYRVLA
                     KPLSSGWEITAVSLDGVESTTRRLVMIEGFGIGALLMGLGLVGWWVIRLGITPMGRMV
                     DASTRIANGDFDVRLEGATSGTESAALATSLNTMIGRLTDALSERERSEARLRVFVAD
                     ASHELRTPLTTVLGYAQLFRKGALARKADQADAWGRTEAEANRMKRLVEDMLELARYD
                     AEPEMHMAPTRRFMRFASASVRPHASAWSALRASAPLRNSCAYPSTVVSGVRN"
     gene            complement(1159..1845)
                     /locus_tag="VFC06_02365"
     CDS             complement(1159..1845)
                     /locus_tag="VFC06_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZL80776.1"
                     /translation="MNQRRILVVDDEDNLRTMLVAALKFEGYQVGQAADGRAGLRAAK
                     ELRPDLIVLDVMMPELDGFGMLKRLRESGDRTPVIFLTAKDTSADAVEGLGLGADDYL
                     AKPFSLEELVARVESVMRRVDASPSASSVMSLADLTLNDVAHEVTRHGEEIHLSPTEY
                     KLVAYLMANTGRVMSRGQLLSNVWGYSPDDDPSVVETYIGYVRKKVDAYEPKLIHTVR
                     GVGYTMKVPQ"
     gene            2053..2685
                     /locus_tag="VFC06_02370"
     CDS             2053..2685
                     /locus_tag="VFC06_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HZL80777.1"
                     /translation="MSVFVFIAADLVAMAVLVFGLYYPRHRRRDMIAPLLAINVGVLG
                     VTYAMATAQLSLGFGLGIFAVLSIIRLRSAEMDHAEIAYYFTGIALGLLGGFPSISIS
                     VSFTLMAVLLLVLTVGDSSRLFANNRQHILVLDRAVPHEGDAKALAEAVLGATVKRIT
                     IRKVDFVLDTTVCDVRYEVGTPQAPDSPPMESLPTSSVPTSSGAGAEVSR"
     gene            2682..>3405
                     /locus_tag="VFC06_02375"
     CDS             2682..>3405
                     /locus_tag="VFC06_02375"
                     /inference="COORDINATES: protein motif:HMM:NF020916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HZL80778.1"
                     /translation="MSWTSIVASLNAVSLDAMNEEAALQTRMDRKYIVDGDTWAGVLV
                     EHAADLRALDVSGSRAAHYRSLYYDTPEMASYMGAARQRPRRFKVRVREYVDSGTSVV
                     EVKLRTPRGATVKHRQLADGIPTAGDVIAFASSFDLIAPYAPDLAARLQTTYTRTTLL
                     HPQGRITIDAQVIASDATGNHDAVDYGSRLIIETKCIGAAGDIDRSLWARGIRPVQLS
                     KYATSMAALHPDLPANRWHRTLT"
BASE COUNT          533 a         1205 c         1099 g          568 t
ORIGIN      
        1 agttgcgcac gccgctcacc acagtgctgg gatacgcgca gctgttccgc aagggtgcgc
       61 tggcgcgcaa ggcggaccac gccgatgcgt ggggccgcac cgaggccgag gcgaaccgca
      121 tgaagcgcct ggtgggggcc atgtgcatct ccggctcggc gtcataacgg gccagttcca
      181 gcatgtcctc caccaggcgc ttcatgcggt tcgcctctgc ctcggtgcgg ccccacgcat
      241 cggcctggtc cgccttgcgc gccagcgcac ccttgcggaa cagttgcgcg tatcccagca
      301 ctgtggtgag cggcgtgcgc aactcatggg aggcatcggc cacaaacacg cgcagcctgg
      361 cctccgagcg ctcccgctct gacaaggcgt cggtgagacg accgatcatg gtgttcagcg
      421 acgtcgcgag tgcggcggac tctgtcccgc tcgtcgcgcc ctcgagccgc acgtcaaagt
      481 cgccgttggc aatccgcgtg gacgcgtcga ccatgcggcc catcggggtg atgcccagtc
      541 gaatcaccca ccacccgacc aggccaagac ccatgagcag cgccccgatg ccgaagccct
      601 cgatcatcac gagccgccgg gtggtcgact cgacgccatc gagtgacacc gccgtgatct
      661 cccagccgct cgacagcggc ttggccagca cgcggtactc atggccaccc gcggaggccg
      721 cggtgatgta ggtcagggag ccgcggctca gatccgccgg attgatgagc ggggcgtcga
      781 acgtctcgtc gctgtagacg ttgggctgga actgcaccac gagcgttccc gtgtcggtga
      841 gcacgccgcg gaccgcgtcg gacgggcgct cgttccacca gtcagtggtg gcggtcgacg
      901 gcgtcgcgat gcttccctcg ccgtcctctt cggcggcgct cggcaccgga tagtcgtccg
      961 cgtcgcccag cccggtcccg tgcgacgggc ccgagaaggc cagtagccgg tcgtccacct
     1021 ggctcaccag gtagctctgc gtggtgagag tcacggccgc tgcggccagg accgcgacca
     1081 aagcgatcgc agccaagccc accagcacgc gggcgcgcag cgagagcgca cgagtgcgcg
     1141 tccctgggtg ggcagcggtc attgcggcac tttcatggtg tagccgacgc cacgcaccgt
     1201 gtggatcagc ttgggctcgt acgcgtcgac cttcttgcgc acgtagccga tgtacgtctc
     1261 gaccaccgaa gggtcgtcgt cgggcgagta gccccacacg ttgctgagga gctgcccgcg
     1321 cgacatgacg cgcccggtgt tggccatgag gtacgccacc aacttgtatt cggtgggcga
     1381 caggtgaatc tcttcgccgt ggcgcgtgac ctcgtgggcc acatcattca gcgtcaggtc
     1441 ggcaagcgac atcacggacg atgcgctggg cgaggcgtcc acgcgacgca tcacggactc
     1501 gacgcgcgcc accaactcct cgagcgagaa cggcttggcg aggtagtcgt cggcgccaag
     1561 gcccaggccc tcgacggcgt cggcggaggt gtccttggcg gtgaggaaga tgacgggcgt
     1621 gcggtcgccc gattcgcgga gccgcttgag catgccaaag ccgtccagct ccggcatcat
     1681 gacgtccagc acgatgaggt cggggcgcaa ctccttcgcg gcacgcaggc ccgcccgccc
     1741 gtcggcggcc tgacctacct ggtaaccctc gaatttgagg gcggccacga gcatggtgcg
     1801 gaggttgtct tcgtcgtcca ccacgaggat gcgtcgctgg ttcatgggac tcagtcaagc
     1861 acccgatgct ccattttcgc gcagagtgcg cgcggaggcg tctgagtccc gccggctttg
     1921 catcgccttg ccaaccgccc ggggcgcccg ctcaggttca tctgcagtcg ctctgcgcta
     1981 cctctgaggt ccgtcgagtg acatagagcc tatgaggcac caccgcggtg cccgcctttc
     2041 caggaggttc tcatgtcagt cttcgtcttc atcgccgcag accttgtcgc catggcggtc
     2101 ttggtctttg ggctgtacta cccccgccac cgcaggcgcg acatgatcgc cccgttgctc
     2161 gccatcaacg tcggcgtgct cggcgtcacg tacgcgatgg cgaccgccca actgtcactc
     2221 ggcttcggcc tcggcatctt cgcggtgctg tcgatcattc gcctgcggtc cgcagagatg
     2281 gaccacgcgg agatcgcgta ctacttcacc ggaatcgcgc tcggcctgct gggcggcttc
     2341 ccgtcgatct cgatcagcgt gagcttcacc ctcatggcag tgctgctgct ggtgctcacg
     2401 gtgggcgaca gttccaggct gttcgccaac aaccgccagc acatcctggt gctcgaccgc
     2461 gccgtgccgc atgagggaga cgccaaggcc ctcgccgagg cagtgctggg cgccaccgtc
     2521 aagcgcatca ccatccggaa ggtggacttc gtgctggaca cgaccgtgtg tgacgtccgc
     2581 tacgaggtcg gcacgccgca ggcccccgac tcgcccccga tggaaagcct gcccacgtcg
     2641 tccgtgccga cctcgtccgg tgcgggcgcc gaggtgtcgc gatgagttgg accagcatcg
     2701 tcgcctcgct caacgcggtc tccctcgatg cgatgaacga ggaggccgcg ctccagaccc
     2761 gcatggaccg caagtacatc gtggacggcg acacctgggc gggggtgctc gtcgagcacg
     2821 ccgccgacct ccgcgcgctc gacgtttccg gctcccgagc cgcccactac cgctcgctgt
     2881 actacgacac cccggagatg gcgagctata tgggggccgc ccgtcagcgc ccgcgccgct
     2941 tcaaggtgag ggttcgcgag tacgtggact ccggcacctc tgtcgtggag gtgaagttgc
     3001 gcaccccgcg cggagccacc gtgaagcacc gtcagttggc tgacggcatc ccgacagccg
     3061 gagacgtcat cgcctttgcc tcgtccttcg acctgattgc cccgtacgcg ccagacctcg
     3121 cggcgaggct gcagaccacg tacacgcgga ccaccctgct gcatccgcag ggccgcatca
     3181 cgatcgatgc ccaggtcatc gcgtcggacg ccacgggcaa tcacgacgcc gtcgactatg
     3241 gctcgcgcct catcatcgag accaagtgca tcggcgcggc tggcgacatc gaccgctccc
     3301 tgtgggcccg tggcattcgc ccggtgcaac tgtccaagta cgcgacctcc atggccgcgc
     3361 tccaccccga tctccccgcc aaccgctggc accgcacctt gaccc
//
LOCUS       DASRKH010000140         4029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_35033, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000140 DASRKH010000000
VERSION     DASRKH010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35033"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="VFC06_02380"
     CDS             <1..513
                     /locus_tag="VFC06_02380"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HZL80779.1"
                     /translation="TLADSLAGVLTLLWIPFLASFLYLLELADDGWQRVFIVVLAVVG
                     NDTGALVFGMLFGKHKLLERVSPAKTWEGAIGGVFVGTAAASVAAWFFFEGQWYIGAA
                     VGAAATLAAIVGDLAESALKRDIRVKDMSQALPGHGGILDRIDSLLFAAPVGYVVFAI
                     FLGTLGGGGL"
     gene            510..1634
                     /gene="rlmN"
                     /locus_tag="VFC06_02385"
     CDS             510..1634
                     /gene="rlmN"
                     /locus_tag="VFC06_02385"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HZL80780.1"
                     /translation="MTDAVKPPVIQMAAPKRGKPPVHFADLGADERAAAVKELGQPAF
                     RASQLNTHYFTHLTVDPDEMSDLPADARVAMAEALFPPLMTPVRTLEADKGATVKTLW
                     TLHDGVKVESVLMKYPERATLCVTSQAGCGMACPFCATGQMGLTRNLSAAEIIEQVRL
                     AARASRDGVLGGDPVRLSNVVFMGMGEPLANYKAVMTAVRALVAPAPHGFGLSARHVT
                     VSTVGLVPAIDKLTAEGIPVTLAVSLHAPDDELRNELVPINNRYSVDQTLDAARRYFD
                     ATGRRVSIEYALIRDMNDQAYRADLLGRKLMERGTGWVHVNPIPLNPTPGSIWTASDP
                     KVEKAFVDTLASHGIPVTIRDTRGSDIDGACGQLAIKEES"
     gene            1631..2188
                     /locus_tag="VFC06_02390"
     CDS             1631..2188
                     /locus_tag="VFC06_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HZL80781.1"
                     /translation="MRDLFPHAGFMRTGYHLVQVDEFFDRARAAYERPMLDDGGLSAL
                     DVRRAAFDITRGGYKTAAVDAALDRLETAFAARAREQFVRAHGHEAWMNQLAERAQVL
                     YPRLRRPKGERFRQPVTSRHGYDAREVDALIDRLIAFFDTGEQLTAEDLRHATFKRRG
                     KRGAYDERTVDAFVARAVDVLMGAQ"
     gene            2185..3351
                     /gene="dxr"
                     /locus_tag="VFC06_02395"
     CDS             2185..3351
                     /gene="dxr"
                     /locus_tag="VFC06_02395"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HZL80782.1"
                     /translation="MTRRTVALLGSTGSIGTQALDVIGRNPDLFEVTALAAGGSDPAA
                     IAAQAAATRPAIVAVANAAAVDDVRSALSAAGVHGVEVTGGAQAVVDAAASGADVVLN
                     GMTGSVGLRPTLAALAVGSTLALANKESLVAGGALVKAALQRADQIVPVDSEHSALAQ
                     ALRGGSRGEVKRLVLTASGGAFRGLIREELAHVTVEQALKHPNWLMGPVVTINSASLV
                     NKALEVIEAHWLFDMPYAQIDVVIHPQSIVHSMVEFVDGSTIAQASPPDMRLPIALGI
                     GWPDRVADAIPGLDWSRASAWEFEPLDDDAFPAISLARHAAAASDLHPAVFNAANEQC
                     VAAFVAGALPFLGIVDTVRAVVEEYSAPGDVTLEAVDAAESWARARADALIAAR"
     gene            3380..>4029
                     /locus_tag="VFC06_02400"
     CDS             3380..>4029
                     /locus_tag="VFC06_02400"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HZL80783.1"
                     /translation="MLQVLFIVVFAIGLMLSIALHEMGHLIPAKRFGVKVSEYFVGFG
                     STLWSTKRGETTYGIKAFPLGGYVRLVGMVPPGDVVKPVKVHGWMRDLIDDARASSEV
                     EIGDDRDRAFYRLSWWKKAVVMFGGPAVNLAIAALLFTVVFSGIGSHESTLTVRDTVA
                     CVPATGTVDCAPGDPVSPAVAAGIQPGDTFVSVDGVTVNSWADVADYVSQRPGETVA"
BASE COUNT          637 a         1301 c         1375 g          716 t
ORIGIN      
        1 accctcgccg actccttggc aggtgtgctg acgctgctgt ggatcccctt cctcgcatcg
       61 ttcctgtact tgctggagtt ggcggacgac ggttggcagc gcgtgttcat cgtggtgctg
      121 gcagtggtcg gcaacgatac cggcgcgctg gtgtttggga tgctgttcgg aaagcacaaa
      181 cttctcgagc gagtgagccc tgccaagacg tgggagggcg ccatcggcgg cgtgttcgtc
      241 ggcaccgctg cggcctctgt tgccgcgtgg ttcttctttg agggccagtg gtacattggc
      301 gcagccgtgg gcgctgcggc gacccttgcc gcgattgtgg gagaccttgc ggagagtgcg
      361 ctcaagcgcg acatccgggt gaaggacatg tctcaagcgc tcccgggtca cggcgggatt
      421 cttgaccgga tcgactcgtt gttgttcgcc gcacccgtgg gttacgtggt gttcgcgatc
      481 ttccttggca cgttgggagg cggtggccta tgacagacgc tgtgaagccg cccgtcatcc
      541 agatggccgc gccgaagcgg ggcaagccgc ccgtgcactt cgccgacctt ggcgccgacg
      601 agcgtgcggc cgccgtcaag gagttggggc aacccgcgtt cagagcgtcg cagttgaaca
      661 cccactactt cacacacctc accgttgatc ccgacgagat gagcgacctg ccggccgatg
      721 ctcgcgtggc aatggcggag gcgttgttcc cgccgctcat gactccggtg cgcacgctcg
      781 aagccgacaa gggggccacg gtcaagacgt tgtggacact tcacgatggc gtcaaggtcg
      841 aatccgtcct catgaagtac ccggagcggg ccactctgtg cgtgaccagc caggcgggtt
      901 gcggcatggc gtgtccgttc tgcgcgacgg gtcagatggg actcacgaga aacctctccg
      961 ccgcagaaat catcgaacag gtgcgcctcg cagcgcgcgc ctcgcgcgac ggagtgctcg
     1021 gcggagatcc cgtgcgactg tcgaacgtgg tgttcatggg catgggcgag ccgctagcca
     1081 actacaaggc ggtcatgacg gcagtgcgtg cgctggtggc gccggccccg cacggtttcg
     1141 ggctttccgc gcgccacgtc accgtgtcga cggtgggact ggtgccggca attgacaagc
     1201 tgacggccga ggggattcct gtgacgttgg ccgtgtctct ccacgcgccg gatgacgagc
     1261 tgcgcaacga gttggtgccc atcaacaacc gttacagcgt ggaccagacg ctcgacgctg
     1321 cgcgcaggta tttcgacgcc accggcaggc gcgtcagcat cgagtacgcg ctcatccgtg
     1381 acatgaatga ccaggcatac cgcgccgacc tcttggggcg caagctcatg gagcgcggaa
     1441 ccgggtgggt tcacgtcaac cccattccgc tgaacccgac cccgggcagc atatggacgg
     1501 catccgaccc caaggtcgag aaagctttcg tcgacacgct cgcgagtcac ggaatcccgg
     1561 tcaccatccg cgatactcgt ggaagcgaca tcgatggcgc atgtggccaa ttggccatca
     1621 aggaggagtc atgagggacc tgtttccgca cgccggattc atgcggaccg ggtaccacct
     1681 cgttcaggtg gatgagttct tcgaccgggc gcgagccgcc tatgagcggc cgatgcttga
     1741 tgacggcgga ttgtccgcgc tcgacgtgcg tcgtgccgcg ttcgacatca cgcgtggcgg
     1801 ctacaagacc gccgctgtgg atgccgccct cgatcgactc gagaccgcgt tcgcggcgcg
     1861 tgcgcgcgaa caattcgtcc gtgcgcatgg ccacgaggct tggatgaacc agctcgctga
     1921 gcgcgctcag gtgctgtacc ccagactgcg ccgacccaag ggcgagcgct ttcgtcagcc
     1981 cgtcacatcg cggcacggct acgacgcccg cgaggtcgat gccctcatag atcggctgat
     2041 cgccttcttc gacaccggcg aacaactcac cgctgaggat ctcaggcacg ccacgttcaa
     2101 gcgtcgcggg aagcgcggcg cttacgacga acgcaccgtc gacgctttcg tggcgcgtgc
     2161 ggtcgacgtg ctgatgggcg cccagtgacc cgtcgcactg tcgcgctgct gggatccacg
     2221 ggttccatcg gcacacaggc gctcgacgtg atcggccgca accccgacct cttcgaagtc
     2281 accgcgcttg cggcgggcgg cagcgatccc gctgcgatcg ccgcccaagc cgcggccacg
     2341 cgtccggcga tcgtggcggt cgcgaacgcc gcagcggtgg acgacgtgcg gtccgccttg
     2401 agcgccgcag gcgtccacgg cgtcgaagtg acgggcggcg cccaggccgt ggtggacgcg
     2461 gcggcatcgg gcgcggacgt ggtgctgaat ggaatgaccg gctcggtggg cctgcggccc
     2521 acgctggccg ccctcgccgt gggctcgact ctcgcactcg ccaacaagga gtcgctggtg
     2581 gctggcggag cgctcgtcaa ggctgcgctg cagcgagcag accagatcgt gccggtcgac
     2641 tcggagcatt ccgcgctcgc acaggccctg cgaggcggtt cccggggcga ggtcaagagg
     2701 ctcgtgctca cggcatcggg cggcgccttc aggggcctga tcagagaaga actggcccac
     2761 gtgaccgtgg aacaggcgct caagcacccc aactggctca tgggccccgt ggtgaccatc
     2821 aactcggcga gcctggtcaa caaggcgctt gaggtgatcg aggctcactg gctcttcgac
     2881 atgccgtacg cacagatcga tgtggtgatt caccctcaat cgatcgtgca ctccatggtc
     2941 gagtttgtgg acggatccac gatcgcgcag gcctcaccac ccgacatgcg cctcccgatc
     3001 gcgctcggaa tcggatggcc cgatcgcgtg gcggacgcga ttccggggct tgactggtcc
     3061 cgcgcatcgg cgtgggagtt tgagcccttg gacgacgacg cttttcccgc gatttccctg
     3121 gcccgacacg cggccgcggc gtcggacctg catccagccg tcttcaacgc ggcgaacgaa
     3181 cagtgcgtcg cggcgtttgt tgcaggagcg ctgccgttcc tgggcatcgt cgacacggtg
     3241 cgtgccgtgg tggaggagta cagcgccccc ggggacgtca ctctcgaagc cgtggacgcg
     3301 gcggagtcgt gggcgcgggc gcgggcagac gccctcatcg ccgcccgcta acgtcccggc
     3361 actggcttac agtggtgcca tgctccaggt gctcttcatc gtcgtgttcg cgatcggtct
     3421 catgctgtcc atcgccctgc acgaaatggg ccacctgatc ccggccaagc gctttggcgt
     3481 caaggtcagc gaatatttcg tgggcttcgg ctcgacgctg tggtccacga aacgaggcga
     3541 gaccacgtac ggcatcaagg ccttcccgct gggcggctac gtacggctcg tgggcatggt
     3601 gcccccgggc gacgtggtga agccggtcaa ggtgcacggc tggatgcgcg acctgatcga
     3661 cgacgccagg gcgtcctctg aagtggagat cggcgacgac cgcgaccggg ccttctatcg
     3721 cctgtcttgg tggaagaagg cggtcgtgat gtttggcggc cccgccgtca acctggcgat
     3781 tgcggcgctc ctattcaccg tcgtcttctc cgggatcggg tctcatgagt cgaccctgac
     3841 ggtgcgcgac accgtggcgt gcgtgcccgc taccggcacc gtcgattgcg cgccggggga
     3901 ccccgtgtcg cccgcggtcg ccgccgggat tcaaccggga gacacgttcg tgtcggtgga
     3961 tggcgtcacg gtcaactcgt gggcagatgt ggccgactac gtgtcgcagc ggccgggcga
     4021 gaccgttgc
//
LOCUS       DASRKH010000141         3178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_11894, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000141 DASRKH010000000
VERSION     DASRKH010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11894"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..322
                     /gene="rpsQ"
                     /locus_tag="VFC06_02405"
     CDS             35..322
                     /gene="rpsQ"
                     /locus_tag="VFC06_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011775587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HZL80784.1"
                     /translation="MSSKKHATATVEHRTQRKTRRGYVVSDKMEKTITVEVEDRVKHA
                     LYGKVLRRSSKVKAHDETNTAGIGDLVLIMETRPMSASKRWRLVEILEKAK"
     gene            384..755
                     /gene="rplN"
                     /locus_tag="VFC06_02410"
     CDS             384..755
                     /gene="rplN"
                     /locus_tag="VFC06_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HZL80785.1"
                     /translation="MIQQESRLRVADNTGAKEILCIRVLGGSHRRYAGIGDVIVATVK
                     DAIPGGNVKKGEVVKAVIVRTVKETRRTDGSYIKFDENAAVILKGKDTDPRGTRIFGP
                     VGRELRDRKFMKIVSLAPEVI"
     gene            757..1098
                     /gene="rplX"
                     /locus_tag="VFC06_02415"
     CDS             757..1098
                     /gene="rplX"
                     /locus_tag="VFC06_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HZL80786.1"
                     /translation="MAKIKKGDLVVVIAGRDRGQQGHVLEVLRDEDRVVVEGIQRVQR
                     HIKPGTRRDNMEGGIVTVESPLHLSNVMLVDPETKKGTRVGFRAVQVERDGRTRTKRV
                     RVAKRSGKDID"
     gene            1098..1661
                     /gene="rplE"
                     /locus_tag="VFC06_02420"
     CDS             1098..1661
                     /gene="rplE"
                     /locus_tag="VFC06_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HZL80787.1"
                     /translation="MATATTSQARLKARYRAEIVAALNEQFGYTNINQVPGLTKVVVN
                     MGVGDAAKDSKLMEGAIRDLTAITGQKPQVTKARKSIAQFKLREGQPIGAHVTLRGDR
                     MWEFLDRLLSTALPRIRDFRGLSPKQFDGHGNYTFGLTEQSMFHEIDQDKIDRVRGMD
                     ITVVTTATNDDEGRSLLKQLGFPFKEK"
     gene            1664..1849
                     /locus_tag="VFC06_02425"
     CDS             1664..1849
                     /locus_tag="VFC06_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HZL80788.1"
                     /translation="MAKTSLKVKAGRKQKFAVRAYTRCQKCGRPHSVYRKFGLCRVCF
                     REMAHRGELPGITKSSW"
     gene            1904..2302
                     /gene="rpsH"
                     /locus_tag="VFC06_02430"
     CDS             1904..2302
                     /gene="rpsH"
                     /locus_tag="VFC06_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003974254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HZL80789.1"
                     /translation="MTMTDPIADMLTRLRNANMAYHNSVSMPHSKLKANIAKILEEQG
                     YINGSAVADAEVGKTLTLSLKYGPNRERSLAGVRRISKPGLRVYAKSTNLPKVLGGLG
                     VAILSTSSGLLTDREAEKKGVGGEVVAYVW"
     gene            2320..2859
                     /gene="rplF"
                     /locus_tag="VFC06_02435"
     CDS             2320..2859
                     /gene="rplF"
                     /locus_tag="VFC06_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HZL80790.1"
                     /translation="MSRIGKLPVPVPSGVDVTIDGAAVTVKGPKGTLQHTVASPIVAE
                     LGEAVIDVKRPNDERISKSLHGLTRTLIANMVTGVTEGYEKKLEIVGTGYRVVAKGTD
                     LEFALGFSHPVFVSAPEGITFTVESPTKFAVSGIDKQQVGEVAANIRKIRKPEPYKGK
                     GVRYAGELVRRKAGKTGKK"
     gene            2859..>3178
                     /gene="rplR"
                     /locus_tag="VFC06_02440"
     CDS             2859..>3178
                     /gene="rplR"
                     /locus_tag="VFC06_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HZL80791.1"
                     /translation="MGITVKGKGKTISRTRRHFRLRKKIAGTSVRPRLVVTRSARHMV
                     AQVVDDTAGTTLVSASTLEVDVRAMKGDKTAKATKVGQLVAERAKSAGIEAVVFDRGG
                     NKYHG"
BASE COUNT          684 a          968 c          996 g          530 t
ORIGIN      
        1 cccgcggcta agtcgtaagt caagaggtaa aaatatgtcg agcaaaaagc acgctacggc
       61 gacagtagag caccgcacgc aacgcaagac gcgtcgcggc tacgtcgtgt cagacaagat
      121 ggagaagacc atcaccgttg aggtcgaaga ccgcgttaag cacgcgctct acggcaaggt
      181 tttgcgccgt tcctcgaagg tcaaggcgca cgacgaaacg aacaccgccg gtatcggcga
      241 cctcgtgctc atcatggaga cccgcccgat gtcagcgtcc aagcgctggc gcctggtgga
      301 gatcctcgag aaggccaagt aaccaacgtt cgaccaggct cgccgctcac ggcgacgaga
      361 accagacgac aggagaagaa gacatgattc agcaggagtc gcgactgcgt gtcgccgaca
      421 acacgggtgc caaggagatc ctctgcatcc gcgttctcgg cggatcgcac cgtcgctacg
      481 ccggcatcgg tgacgtcatc gttgccacgg tcaaggatgc aatccccggc ggaaacgtca
      541 aaaagggtga agtcgtcaag gccgtgatcg ttcgcaccgt caaggagacc cgtcgtaccg
      601 acggttcata catcaaattc gatgagaacg ccgccgtcat cctcaagggc aaggacacgg
      661 accctcgcgg gacgcgcatc tttggccctg tgggtcgcga gctgcgtgac cgcaagttca
      721 tgaagatcgt gtcgcttgct ccggaggtga tctgacatgg cgaagatcaa gaagggcgac
      781 ctcgtcgtcg tcattgcagg acgtgaccgt ggccagcagg gccacgtgct ggaagtgctg
      841 agggacgagg accgtgtcgt cgtcgaaggc atccagcgcg tacagcgtca catcaagccg
      901 ggaacgcgtc gcgacaacat ggagggtggc attgtcaccg tcgagtcgcc gctgcatctg
      961 tccaacgtga tgctcgttga cccggagacc aagaagggca ctcgcgtggg cttccgcgcg
     1021 gtgcaggtcg agcgtgacgg ccgtactcgc accaagcggg tacgcgtcgc caagcgctcg
     1081 ggtaaggaca tcgactgatg gcgaccgcta cgacatccca ggctcgcctc aaggcgaggt
     1141 accgcgcaga gatcgttgct gcgctcaatg agcagttcgg ctacacgaac atcaaccagg
     1201 ttcccggtct caccaaggtc gtcgtgaaca tgggtgttgg cgacgcagcc aaggacagca
     1261 agctcatgga aggtgccatc cgcgacctga cggccatcac cggccagaag ccgcaggtga
     1321 cgaaggcccg caagtccatc gcgcagttca agctgcgtga aggtcagccg atcggcgcgc
     1381 acgtgacgct gcgcggcgac cgcatgtggg aattcctcga ccgactgctc tcgactgcat
     1441 tgcctcgtat ccgcgacttc cgtggtctga gccctaagca gttcgatgga cacggcaact
     1501 acacgtttgg cctcacggag cagtcaatgt tccacgagat cgatcaagac aagattgacc
     1561 gggtgcgcgg catggacatc actgttgtca cgaccgccac caacgacgac gagggtcgct
     1621 cgctgctcaa gcagctgggc ttccccttca aggagaagta gcgatggcga agacctccct
     1681 caaggtgaag gcgggccgca agcagaagtt tgcggtgcgt gcttacaccc ggtgccagaa
     1741 gtgcggacgt ccgcactccg tgtaccgcaa attcggcctc tgccgcgtgt gcttccgtga
     1801 aatggcgcac cgtggcgagc tccccggcat caccaagagc agctggtaaa ccactacgcc
     1861 gcaggtcctt cgggaaacca cgacgaggaa gagtgactca ctcatgacaa tgaccgaccc
     1921 gatcgcagac atgctcacgc ggctgcgcaa cgccaacatg gcgtaccaca actcggtgtc
     1981 catgccccat tcgaagctca aggccaacat cgccaagatc ctcgaggagc agggatacat
     2041 caatggctcg gcagttgccg acgccgaggt gggcaagacg ctcacgctgt ctctgaagta
     2101 cggccccaac cgtgagcgtt cgctcgcggg ggtccgccgc atctcgaagc ccggacttcg
     2161 cgtttacgcg aagtcaacca acctccccaa ggttcttggt ggactcggtg tggcgattct
     2221 gtccacttcc tccggtctgc tgaccgaccg cgaggccgaa aagaagggcg tgggcggcga
     2281 agtcgttgcc tacgtctggt agccggaaag gagagattta tgtctcgcat tggcaagctc
     2341 cccgttccgg ttccctccgg agtggatgtc accatcgacg gcgctgccgt gactgtgaag
     2401 ggccccaagg gcactctgca gcacaccgtg gcgtctccca tcgtcgccga attgggcgaa
     2461 gcagtgatcg atgtcaagcg cccgaacgat gagcgcatct ccaagtcgct tcacggcctg
     2521 actcgcacgc tgatcgccaa catggtgacc ggtgtgaccg agggctacga gaagaagctc
     2581 gagattgttg gcaccggtta tcgcgtagtg gcgaagggca cggacctcga gttcgccctg
     2641 ggcttctcgc accctgtctt cgtctcggcg cctgagggaa tcacgttcac cgtggagtcc
     2701 cccaccaagt tcgccgtgtc gggaatcgac aagcagcagg tgggcgaagt cgcggccaac
     2761 attcgcaaga ttcgcaagcc cgagccttac aagggcaagg gtgtccgcta cgcaggcgaa
     2821 ctggttcgcc gcaaggccgg aaagacgggt aagaagtaat gggtatcacg gtgaagggca
     2881 aggggaagac gatctcgcgt acgcgtcgtc acttccgcct gcgcaagaag atcgccggca
     2941 cgtccgtccg cccgcgcctc gtggtgacgc gttccgcgcg tcacatggtg gcgcaggttg
     3001 tggacgacac ggcgggcacg acgctcgtat cggcgtcgac cctcgaggtc gacgtgcgcg
     3061 cgatgaaggg cgacaagacg gccaaggcca ccaaggtcgg ccaactcgtc gctgagcgcg
     3121 ccaagtctgc aggcatcgag gcggtcgtgt tcgaccgtgg cggaaacaag taccacgg
//
LOCUS       DASRKH010000142         2145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_63276, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000142 DASRKH010000000
VERSION     DASRKH010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63276"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="VFC06_02445"
     CDS             <1..853
                     /locus_tag="VFC06_02445"
                     /inference="COORDINATES: protein motif:HMM:TIGR03062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HZL80792.1"
                     /translation="GMTTLATGASALAAGTSTLAGGATQVADGTVQLEDGAQTLADGL
                     STGAVAIPDDSTGVRTQRAATLASPVALESSSIAAAEGFGEGFAPFFIPLALFVGSLI
                     TWLLLRPLPSRALATPASGRRATLAGFLPAMLIGVGQVAVMLAVIHYGVGLQMATTGG
                     TIAFTLLIVAAFLALQQMLTAVMGPAAGKVAILALLMLQLASSGGTYPVETTPAFFQA
                     IHPLLPMSYAVTGLREVITGGMDARLWGAVAYLGAVLIGSLAITSWRAGQLRTWSLER
                     LHPAITI"
     gene            866..1498
                     /locus_tag="VFC06_02450"
     CDS             866..1498
                     /locus_tag="VFC06_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZL80793.1"
                     /translation="MTTSATPDPAPAPPPSPVPVPGAPDTAPPRRGTRGSIVDAAVAL
                     IADRGFSATSVDDIAAAAGVAKGSIYYNFGSKTALFEAVIVEGVDRLTTALRASVVGL
                     DGPRAIEALVTELLIQVQTHPDFAKLVVAEVFRTGRDWQHSIRQVREESMAVFADVVH
                     ATWPDRDPSLTAAAIFGATLVAGLEWLAFQPHRPAADVRAAVLATVLHGS"
     gene            complement(1587..1787)
                     /locus_tag="VFC06_02455"
     CDS             complement(1587..1787)
                     /locus_tag="VFC06_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BldC family transcriptional regulator"
                     /protein_id="HZL80794.1"
                     /translation="MSAPITGQLLTPGEVAVMFRVDPKTVTRWAQAGKLSAVRTLGGH
                     RRFHEAEVRELLTGVPQQRADS"
     regulatory      complement(1837..1911)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          297 a          786 c          742 g          320 t
ORIGIN      
        1 gggcatgacg acgctcgcca cgggcgcgtc cgcgctcgcg gccggcacga gcacgctggc
       61 cggcggcgcg acccaggtcg ccgacgggac ggtccagctc gaggacggtg cgcagacgct
      121 cgccgacggg ctgtcgaccg gtgccgtcgc gatccccgac gactcgaccg gtgtccgcac
      181 ccagcgcgcg gcgaccctcg cgtcacccgt cgcgctcgag tccagcagca ttgccgccgc
      241 cgaggggttc ggcgagggct tcgcgccctt cttcatcccg ctcgccctgt tcgtgggctc
      301 gctcatcacg tggctcctgc tgcgcccgct cccctcgcgg gcgctcgcca cgccggcgtc
      361 cggacggcgt gcgacgctcg ccggcttcct gcccgcgatg ctcatcggcg tcggacaggt
      421 cgcggtgatg ctcgccgtca tccactacgg cgtcgggctg cagatggcga ccacgggcgg
      481 cacgatcgcg ttcacgctgc tgatcgtcgc tgcgttcctc gcgctgcagc agatgctgac
      541 tgccgtgatg ggcccggctg cggggaaggt ggccatcctg gcgctcctga tgcttcagct
      601 cgcgtcgtcg ggcggcacct accccgtcga gacgacaccc gcgttcttcc aggcgatcca
      661 ccccctgctc ccgatgagct acgcggtcac gggactgcgc gaggtcatca cgggcgggat
      721 ggacgcccgg ttgtggggtg ccgtcgccta cctcggggct gtgctcatcg ggtcgctcgc
      781 gatcacgtcg tggcgcgccg ggcagctgcg cacctggagc ctcgaacggc tccacccggc
      841 catcaccatc tgacaggctg cccccatgac gaccagtgcg acacctgacc ctgcaccggc
      901 tcccccgccg tcgccggtgc cggtgccggg tgcgcccgac acggcaccgc cgcggcgcgg
      961 cacgcgcggg agcatcgtcg acgcggccgt cgcgctcatc gccgaccgcg ggttctcggc
     1021 gacgtcggtc gacgacatcg cggccgcggc gggtgtggcg aagggcagca tctactacaa
     1081 cttcggctcc aaaacggcgc tgttcgaggc ggtcatcgtc gagggggtgg atcgcctgac
     1141 aaccgcgctg cgcgcttccg tcgtcggtct ggacgggcca cgggcgatcg aggcgctcgt
     1201 gaccgagctg ctgatccagg tccagaccca ccccgacttc gccaagctcg tcgtggccga
     1261 ggtcttccgg acgggtcgcg actggcagca ctcgatccgg caggtgcgcg aggagtcgat
     1321 ggcggtgttc gccgacgtcg tccacgcgac ctggccggat cgcgacccgt cgctgaccgc
     1381 cgccgcgatc ttcggcgcaa cgctcgtggc cggcctcgag tggctcgcgt tccagccgca
     1441 ccgccccgca gccgacgtgc gggcggcagt gctggccacg gtgctccacg ggagctgacg
     1501 cgaaccgcac gatgcggcgt gcgaacccct gacatgacga cgggcccggg gatcactccc
     1561 caggcccgat gtcatgctgc gaagaatcag ctgtcggcgc gctgctgcgg cacacccgtg
     1621 aggagctcgc ggacctcagc ctcgtgaaaa cgacggtgcc cgccgagggt ccggacggcc
     1681 gagagcttgc cggcctgggc ccagcgcgtg accgtcttcg ggtcgacgcg gaacatcaca
     1741 gccacctcgc ccggggtcaa gagctggcct gtgatcgggg cggacatacc ggcgcggtga
     1801 gatgtggtga gagacatgat gggattgctc ctgtcgttcg gtagctcggc tggccgtgga
     1861 ggccccgtgg ctttgcgtcc ccgcctcgcg gcgggtttgc ccttgttcgc tgacatgacg
     1921 actatggccg aatccggaca tcagtgcaac aaacggccgg gtgaaaacgc acgattcgcc
     1981 cgcgcaaccg ggtgaatcct cgaccagcct gccgacgcga cctgcggccg gcccccggca
     2041 cggcacatat gccgcgagac tcaacaatcg cccagcccgg ccgatgaagc atgaggaccc
     2101 aggcccctgg gaccgagcac cggacggcga cacgtgcccg agacc
//
LOCUS       DASRKH010000143         2061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_32148, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000143 DASRKH010000000
VERSION     DASRKH010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32148"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1484
                     /gene="acnA"
                     /locus_tag="VFC06_02460"
     CDS             <1..1484
                     /gene="acnA"
                     /locus_tag="VFC06_02460"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HZL80795.1"
                     /translation="QVSLTLADGTQTELDHGHVVIASITSCTNTSNPSVMLAAALLAR
                     NAHKRGLTSKPWVKTSMAPGSQVVTDYYEKAGLWPDLEALGFHLVGYGCTTCIGNSGP
                     LPTEISDAVNEHDLAVVSVLSGNRNFEGRINPDVKMNYLASPPLVIAYALAGTMDFDF
                     ETEPLGQDLEGNDVFLRDIWPGPAEVEAVVGSSINESMFSTNYADVFAGDERWRGLPT
                     PDGKTFEWEQDSTYVRKPPYFEGMSAKPEPVQDIAGARVLALLGDSVTTDHISPAGSI
                     KADSPAGAYLADHGVERRDFNSYGSRRGNHEVMIRGTFANIRLRNLLLAASHNGAGVE
                     GGFTHNFLTDAQDTIYDAAMGYADAGVPLVVLAGKEYGTGSSRDWAAKGTRLLGVRAV
                     IAESFERIHRSNLIGMGVLPLQFPEGKSARDLGLDGTETFGIAGVSAFSAGEHPRSLT
                     VVATAADGSTSEFDAVVRIDTPGEAEYFRNGGILQYVLRSLVG"
     gene            1565..>2061
                     /locus_tag="VFC06_02465"
     CDS             1565..>2061
                     /locus_tag="VFC06_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HZL80796.1"
                     /translation="MLERIESPRDVRSLTAAELSELAAEIRKYLVDSVSRSGGHLGPN
                     LGVVELTLALHRVFDSPFDSIVFDTGHQSYVHKLLTGRRDFSTLRKRGGLAGYPSRAE
                     SDHDIVENSHASTSLSWAAGIARGHTLRGETDRTTVAIIGDGALTGGMAWEALNSLAD
                     CNDRVV"
BASE COUNT          357 a          665 c          674 g          365 t
ORIGIN      
        1 agcaggtatc gctcacgctt gcggacggga ctcagaccga gctagaccac ggtcacgtgg
       61 tcatcgcgtc catcacgtcc tgcaccaaca cgtcgaaccc gtcggtgatg ctcgcggcgg
      121 cgctgctggc ccgcaacgca cacaagaggg gtctcacgtc caagccttgg gtgaagacgt
      181 cgatggctcc cggttcccag gtcgtgaccg actactacga gaaggcgggc ctgtggcccg
      241 acctcgaggc gctcggcttc cacctggttg ggtacggctg caccacgtgc atcggcaact
      301 ccggcccgct gcctaccgag atctcggacg cggtcaacga gcacgacctc gccgtggtct
      361 ccgtgttgtc agggaaccgc aacttcgagg gccgcatcaa ccccgacgtc aagatgaact
      421 acctggcgtc gcctccactg gtgatcgcct acgcgttggc ggggacgatg gacttcgatt
      481 ttgagacgga gccgctcggc caggaccttg agggcaacga cgtgttcctg agggacatct
      541 ggcccgggcc ggccgaggtc gaggccgtgg tgggatcgtc gatcaacgag tcgatgttct
      601 ccaccaacta cgccgatgtg ttcgccggcg atgagcgttg gcgcgggctt cccacgcccg
      661 acggcaagac gttcgagtgg gaacaggact cgacgtacgt gcgcaagccc ccatacttcg
      721 aaggcatgag tgcgaagccc gagccggttc aggacatcgc cggcgcccgc gtgctcgcgt
      781 tgctcggcga ctcggtcacc accgaccaca tttcccccgc gggttcgatc aaggccgata
      841 gccccgccgg cgcatacctc gccgatcacg gagttgagcg ccgtgacttc aacagctacg
      901 gctcgcgtcg cggcaaccac gaggtcatga ttcgcggcac ctttgccaac attcgtctca
      961 ggaacctgtt gctagcggca tcccacaacg gcgcaggcgt cgagggcggc ttcacgcaca
     1021 acttcctcac cgatgcccag gacacgattt acgacgccgc gatgggctac gccgatgctg
     1081 gtgttccgct cgtggtgctc gcgggcaagg agtacggaac gggctcaagt cgcgactggg
     1141 cggcgaaggg cacccggttg ctgggcgtgc gagctgtcat cgccgagagc tttgaacgca
     1201 ttcaccggtc gaacctcatc gggatgggcg tgttgccgct gcagttcccg gagggcaagt
     1261 cggcccggga ccttgggctg gacggcaccg agaccttcgg catcgcggga gtgagtgcct
     1321 tcagcgcggg cgagcaccct cggtcactca cggtggtcgc caccgcagct gacggctcga
     1381 cctcggagtt cgacgcggtg gtgcggatcg acaccccagg ggaggccgaa tacttccgca
     1441 acggcgggat tctgcaatac gtgttgcgtt cgcttgtcgg atagtccggt cgcgcggaag
     1501 cgaaactcgt tcgtcagcgt tgtgaggaca tacagtgaga cgacctcgga agtcaggagc
     1561 aagcgtgctg gagcgcattg agtcaccgcg tgatgtgcgc tccctgaccg ccgcggagtt
     1621 gagcgaactc gctgcggaga tccggaagta cttggtggac tccgtgtcgc gttccggcgg
     1681 gcacctcgga cccaacctgg gcgtcgtcga gttgacgctg gctctgcatc gggtgttcga
     1741 ctctcccttc gacagcatcg tgttcgatac gggtcaccag tcttacgttc acaaactcct
     1801 caccggcagg cgcgacttct ctacattgcg caagcgcggg ggactggcgg gttatccgtc
     1861 gcgcgccgaa tccgaccacg acatcgtcga gaactcgcat gcgtccacgt ccctgtcctg
     1921 ggcggctggg atcgcccgcg gccacacctt gcgaggcgag acggatcgaa cgaccgtcgc
     1981 catcatcggc gacggtgcgc tcaccggcgg catggcgtgg gaggcgctca attcgctcgc
     2041 ggactgcaac gaccgcgtgg t
//
LOCUS       DASRKH010000144         3126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_61077, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000144 DASRKH010000000
VERSION     DASRKH010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61077"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="VFC06_02470"
     CDS             complement(<1..480)
                     /locus_tag="VFC06_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80797.1"
                     /translation="MTTGVSFTVRLPIRSTAGQRRVVARRVAVDDALTKHVLGELIES
                     GKERSDPDLLAFSVDVLTEALEATAPERCVGPESVSDHIATAYRALSQYRDIRRTAER
                     RGWAGDKNAMRSVARREDQGVHAHVPLGRKTAFGDDTEAMVDTLLDLVASGEGRVCVD
                     "
     gene            complement(477..2795)
                     /locus_tag="VFC06_02475"
     CDS             complement(477..2795)
                     /locus_tag="VFC06_02475"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HZL80798.1"
                     /translation="MSVQITDKQLLDEIAAAKAQADNEERGRIERAEANTEGRAAKAD
                     SGVDVLRGILGARGWNERAGQIRMTQYVDEAHDDEDGGVPKRVGIVGVVAPVGTGKTL
                     GYLAPALARGERVIIATSTKALQDQIIGEELPRLREDMINAFGRHITYSVMKGKANYA
                     CLQHVDETIARFASHLGTSSDNDLLGGASGDTELDGLVRISRHTRLAMARGDVVNFDT
                     DLMLQRLSEDTRRGVTAKHACPIRRIPLEDCNVCVEGSGAEGHCQKIEQIAKDDRDVW
                     RSAYCRAMLADIVVMNTALLVEEVTKQNNKASPAVPSVLEGVGMVVIDEAHHAPGIVT
                     QSFSREINRKSTIAMARALDKRIADLDIETMTTMFFNSIDAADEEIKKISEFSEYAAK
                     TCNAFAEQASALLDRKARGATETKGKSQAAVTEFRDDVIDTIKEIGGLAARKSVRGET
                     SVHSARLSVDFLPDGSDFTLTAVPIDVSFFRERLVSAMSGVGTHSVRPKSKHKTIVLC
                     SGTMNATTPRLLGFGGGSIVKTVESPFDPQRARLCVPADLPLPKTEEWEAAAWAKAEQ
                     AILATEGRTLFLCTSNKMVQAFSLRAKSLPFTVLKQGDGSRADLVRRFAQDEHSVLVA
                     TMGYWEGIDVPGPALSLVVLDKIPFPVPSDPIATARREWVEAEGGNAFSIVDVGHAST
                     MLAQGSGRLIRSESDAGGVLILDSRLANARYGSMALSQIERGWLFYRSLDPFLDWMKA
                     VTAAHALGDTESVLDYRRDAEPIRARRKEFVR"
BASE COUNT          510 a         1031 c         1031 g          554 t
ORIGIN      
        1 gtcaacacac acccgcccct caccggaggc caccaggtcg agcaaggtat cgaccatggc
       61 ttccgtgtcg tccccgaacg cagtcttgcg tccgagcgga acgtgcgcgt ggacgccttg
      121 gtcctcccgg cgggcgaccg agcgcatcgc gttcttgtcc ccggcccacc cgcggcgctc
      181 ggcggtacgg cgaatgtcgc ggtactgaga cagcgctcgg taggcggtgg cgatgtggtc
      241 agacacggac tctgggccaa cgcaccgctc gggcgccgtc gcttccagcg cctcggtcag
      301 cacgtcgaca gagaatgcca ggaggtcggg gtcggaccgc tccttaccgc tctcgatgag
      361 ttccccgagc acgtgcttgg tgagcgcgtc atcaacggca actcgtcggg caacaacgcg
      421 ccgctgcccc gccgtggacc tgatgggcaa gcgcactgtg aaggaaacac cggtggtcat
      481 cgcacaaact ccttgcgtcg ggcgcgaata ggctccgcgt cccggcggta gtccagaacg
      541 gattcggtgt caccgagcgc gtgagcggca gtgacggcct tcatccagtc gaggaatggg
      601 tcgaggcttc ggtagaacag ccagccgcgt tcgatctgcg acagcgccat ggacccgtag
      661 cgagcattgg caagacgtga gtcaaggatg agcactcccc cagcgtcgga ctccgagcgg
      721 atgagtcggc ctgagccctg ggcgagcatc gtggatgcgt gccctacgtc aacgatggag
      781 aacgcgttgc ctccctcggc ctcgacccac tcgcggcgtg cggtggcaat cgggtcgctg
      841 ggaaccggga agggaatctt gtcgaggacc acgagggaca gtgccggtcc gggcacgtca
      901 atgccctccc agtaccccat cgtggcgacc agcactgagt gctcatcctg cgcgaaccgg
      961 cgaaccaggt ccgcacgcga accgtcaccc tgcttgagca ccgtgaacgg gaggctcttg
     1021 gctcgcagag agaacgcctg caccatcttg ttcgaggtgc acaggaacag ggtgcgaccc
     1081 tcggtggcga ggatggcctg ctctgccttc gcccacgcgg ccgcctccca ctcttcggtc
     1141 ttgggtagcg gcaggtcggc gggcacgcac aggcgcgcac gctgcgggtc gaaaggcgac
     1201 tcgacggtct tgacgatgga cccgccgccg aagcccagca gccgcggcgt ggtggcgttc
     1261 atcgttcccg agcacagcac gatcgtcttg tgcttcgact tgggtcgcac cgagtgcgtg
     1321 ccgacaccgc tcatcgcgga tacgagacgc tcccggaaga aggacacgtc gatcggcacg
     1381 gccgtgagcg tgaagtcgga tccgtcaggg aggaagtcaa cggacagccg cgcggagtgc
     1441 accgacgtct cgccgcgtac agacttgcgt gccgcgagcc cgccgatctc cttgatggtg
     1501 tcgatgacgt cgtcgcggaa ttcggtcacg gcggcctggg acttgccctt ggtctccgtg
     1561 gccccacgag ccttccggtc cagcagcgcg gatgcctgct cggcgaacgc gttgcacgtc
     1621 ttggcggcgt actcggagaa ctcgctgatt ttcttgatct cctcgtcggc cgcgtcgatc
     1681 gagttgaaga acatcgtcgt catcgtctcg atgtccaggt cggcgatcct cttgtcgagg
     1741 gcgcgcgcca tggcgatggt ggacttgcgg ttgatctccc gcgagaacga ctgggtcacg
     1801 atgccgggcg cgtggtgcgc ctcgtcaatc acgaccatgc cgacaccttc gagcacggac
     1861 ggcacggcgg ggctggcctt gttgttctgc ttggtgactt cctcgacgag cagcgcggtg
     1921 ttcatgacga cgatgtcggc gagcatggcc cggcagtacg cggaacgcca cacgtcacgg
     1981 tcgtccttgg cgatctgctc gatcttctgg cagtgccctt cggcgccgga accctccacg
     2041 cacacgttgc agtcttcaag cgggatgcgg cggatcgggc aggcgtgctt cgcggtaaca
     2101 ccacggcggg tgtcctcgct gagacgctgc aacatcaggt ctgtgtcgaa gttgacgacg
     2161 tcgccgcggg ccatggccag gcgggtgtgc cgggagatgc gtaccagtcc gtcaagttcg
     2221 gtgtcgccag acgcgcctcc gaggagatcg ttgtcggagg aggtgccgag gtgggaagcg
     2281 aagcgtgcga tcgtctcatc gacgtgctgg aggcaagcgt aatttgcctt acctttcatc
     2341 accgagtagg tgatgtggcg cccgaaggcg ttgatcatgt cctcgcgcag tcgcggcagt
     2401 tcctcgccga tgatctggtc ctgcaacgcc ttggtcgagg tggcaatgat tacgcgctca
     2461 ccgcgagcca gtgcgggagc caagtagccg agggtcttgc ctgtgccgac gggcgcgacg
     2521 acacctacga taccgacgcg ctttggcacg ccgccgtctt cgtcgtcatg cgcctcgtcg
     2581 acgtactggg tcatccggat ctgtccggca cgctcgttcc aaccgcgtgc gccaaggatt
     2641 cctcgcagca catccacgcc agagtcagcc ttcgccgcac ggccttcggt attcgcttcg
     2701 gcgcgctcga tacgtccgcg ctcttcgttg tcggcctgcg ctttcgctgc ggcgatctcg
     2761 tcgagcaact gcttgtctgt gatctgtacg gacacgtcga cgccactccc tcaagaaccg
     2821 cgcaaaccgt gtgcctgcac cccagaagac taccacacac ttggaggcgg tcagggagcg
     2881 ccacgggccg tgtcgcgtgc tcagagaatc gaatgtgagc cgcacccgtg acccacccgg
     2941 gggcagttgc ccaccgcact ccttggctac gcgcccgcga ctagcgtcgg cgccgtccgc
     3001 caccgcgccg ggagccacca cgtcgacgcg acagtggcgt cctgcgcatc attttgccca
     3061 ggcccgccat gagcccgccc cgacgggagg cgggggcctc tgccggctgc gaggcgccct
     3121 gctggc
//
LOCUS       DASRKH010000145         2577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_2523, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000145 DASRKH010000000
VERSION     DASRKH010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2523"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VFC06_02480"
     CDS             <1..253
                     /locus_tag="VFC06_02480"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HZL80799.1"
                     /translation="SYGPRMDLGSWPTDTEARVVAVDLEHATRLRLSELGLRTGAIVR
                     VTHRAAFGGRVVAIGTDRFAVDGRTCTRIELETPGGARL"
     gene            250..2340
                     /gene="feoB"
                     /locus_tag="VFC06_02485"
     CDS             250..2340
                     /gene="feoB"
                     /locus_tag="VFC06_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HZL80800.1"
                     /translation="MTSCHDASGGLADARTGLAADPSAPSVVLVGNPNVGKSTLFNAL
                     TGARQQVTNAPGTTVEVQLGTWRGLVGTASGAGADEPAAVRVLDLPGTYSLLARSPDE
                     RVTAETVAGRGALGVPDLAVVLVEAGALSRSLYLLAQVAGAGQALVVALTMTDVAATR
                     GVHVDPEQLATLLGVPVVPIDPRTGTGLDRLTRVVSDALTAGVPPVLLPDAAPACACN
                     GDAACEPGCACDGPVDVDLDLDAELASADRLFTWVDGVQRALAGREVTGASGAVPGVP
                     SPARPAPVRTWSDRVDRVLLDPWFGVPVFLAVMWALFQLATAAAAPLMAAVSSLVGGP
                     VAESVRNLLPGPAWVEGFVVDGVLAGVGTVLSFAPLMGIMFLAIAVLEDSGYLARAAF
                     VADRAMRAIGLDGRAMLPLVVGFGCNLPALAATRTLPNARQRLLTGLLVPYTSCAARL
                     TVYILLASVFFPAHAGTAIFLMYVASITLVVAGGLVLRATAFRDLRREPFVLALPAYQ
                     RPRVRALLLSAWVRVRMFVTDAGKIIVITLAVVWVLMAVPVTGGHQIADVPVADSLYG
                     RAALAISPVFAPAGFDDWHASAALLTGFVAKEVVVGSFAQSYAVDAPTDAAVAGDLGS
                     RLRTTFESSSGGHAGAAAAAFMVFVLAYTPCLATVAEQRRLFGSSWTLSALGVQLAIA
                     WTAAVVVFQLGRLM"
     gene            2351..>2577
                     /locus_tag="VFC06_02490"
     CDS             2351..>2577
                     /locus_tag="VFC06_02490"
                     /inference="COORDINATES: protein motif:HMM:NF020581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoC-like transcriptional regulator"
                     /protein_id="HZL80801.1"
                     /translation="MSMLTDVLREARGGATTDRIAVNLGIDAGLAEAAVDHWVRLGIV
                     SRSGDVLGGAGCHGCEPTPSTSPDRLTMPSRT"
BASE COUNT          266 a          975 c          939 g          397 t
ORIGIN      
        1 gtcgtacggt cctcgtatgg acctggggag ctggccgacc gacaccgagg cgcgagtcgt
       61 cgccgtcgac ctcgagcacg cgacccggct gcggctgagc gagctcgggc tgcgaaccgg
      121 tgcgatcgtg cgggtcaccc accgtgccgc gttcggcgga cgcgtcgtgg cgatcggcac
      181 cgaccggttc gcggtcgacg gtcgcacctg cacgcgcatc gagctcgaga ccccgggcgg
      241 tgcccggctg tgacctcgtg ccacgacgcg tccggcggtc tcgccgacgc ccgcaccggt
      301 ctcgccgcgg acccgtccgc accgtccgtg gtgctcgtcg gcaacccgaa cgtcggcaag
      361 tcgaccctct tcaacgcgct cacgggcgct cgtcagcagg tcaccaacgc cccgggcacc
      421 acggtggagg tccagctcgg gacctggcgg ggcctggtcg ggacggcgag cggtgccggg
      481 gcggacgagc ccgcggcggt gcgtgtcctc gacctgcccg ggacgtacag cctgctcgcg
      541 cggtcgcccg acgagcgcgt gaccgccgag acggtcgcgg gccgcggtgc gctcggcgtg
      601 ccggacctcg ccgtcgtcct cgtcgaggcc ggggcgctgt cacgctcgct gtacctgctc
      661 gcccaggtcg cgggcgccgg ccaggccctc gtcgtcgccc tgaccatgac cgacgtcgcc
      721 gccacgcgcg gtgtgcacgt cgaccccgag cagctcgcga cgctcctcgg ggtgcccgtc
      781 gtccccatcg acccgcgcac cggaaccggt ctcgaccgcc tcacgcgcgt cgtctccgac
      841 gcgctcaccg ccggcgtccc gcctgtcctg ctgcccgacg ccgcaccggc ctgcgcgtgc
      901 aacggggacg cagcgtgtga gccgggctgc gcgtgcgacg ggcccgttga cgtcgatctc
      961 gacctcgacg ccgagctggc gagcgccgac cggctgttca cgtgggtcga cggcgtgcag
     1021 cgggcgctcg cgggccgcga agtcaccggc gcgtccggcg ccgtcccggg cgtcccgtcg
     1081 ccggcccggc ccgctcccgt gcgcacgtgg tccgaccgcg tcgaccgcgt gctgctcgac
     1141 ccgtggttcg gcgtcccggt gttcctcgcc gtcatgtggg cgctgttcca gctcgcgacg
     1201 gccgcggcgg cgccgctcat ggcggccgtg tcgtcactcg tcggcggacc cgtcgcggag
     1261 agcgtgcgga acctgctgcc cgggcccgcg tgggtcgagg gcttcgtggt cgacggcgtc
     1321 ctcgcgggcg tcgggaccgt gctgtcgttc gcgccgctga tggggatcat gttcctggcg
     1381 atcgcggtgc tcgaggacag cggctacctc gcgcgggccg cgttcgtcgc ggaccgggcc
     1441 atgcgggcca tcgggctcga cgggcgtgcg atgctcccgc tcgtcgtcgg tttcggctgc
     1501 aacctgccgg ccctggccgc gacccgcacg ctgcccaacg cgcgacagcg gctgctcacc
     1561 gggctgctcg tgccgtacac gtcgtgcgcg gcccgtctga ccgtctacat cctgctcgcg
     1621 agcgtgttct tcccggcgca cgcgggcacg gccatcttcc tcatgtacgt cgcgagcatc
     1681 acgctcgtcg tcgccggcgg cctcgtgctg cgcgcgaccg cgttccgcga cctgcgccgc
     1741 gaaccgttcg tcctcgcact gcccgcctac cagcgcccgc gcgtccgtgc gctgctgctc
     1801 tcggcgtggg tccgcgtgcg gatgttcgtg accgacgccg gcaagatcat cgtcatcacg
     1861 ctcgcggtcg tctgggtgct catggccgtc ccggtcacgg gtgggcacca gatcgccgac
     1921 gtccccgtcg cggacagcct gtacggccgg gccgcgctgg cgatctcccc ggtgttcgcg
     1981 ccggccgggt tcgacgactg gcacgcgtcc gcggcgctcc tgaccgggtt cgtcgcgaag
     2041 gaggtcgtcg tcgggtcgtt cgcgcagtcc tacgcggtcg acgcgcccac cgacgcggca
     2101 gtggccggcg acctgggcag ccgcctgcgt acgacgttcg agtcgtcgtc cggtggacat
     2161 gccggggccg ctgccgcggc gttcatggtc ttcgtgctcg cctacacccc gtgcctcgcg
     2221 accgtcgcgg agcagcgccg gctgttcggg tcgagctgga cactcagtgc gctcggcgtg
     2281 cagctcgcga tcgcgtggac ggccgccgtc gtggtcttcc agctcggccg gttgatgtga
     2341 cgggccgacg atgagcatgc tgaccgacgt cctgcgcgag gcgcgcggcg gggccacgac
     2401 cgaccggatc gccgtcaacc tcgggatcga cgccgggctg gcggaggcgg ccgtcgacca
     2461 ctgggtgcgg ctcggcatcg tgagtcgatc gggcgacgtg ctaggcggcg cgggctgtca
     2521 cgggtgcgag ccgacgccta gcacgtcgcc cgatcgactc acgatgccga gccgcac
//
LOCUS       DASRKH010000146         2409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_13983, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000146 DASRKH010000000
VERSION     DASRKH010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13983"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..703)
                     /locus_tag="VFC06_02495"
     CDS             complement(116..703)
                     /locus_tag="VFC06_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 domain-containing protein"
                     /protein_id="HZL80802.1"
                     /translation="MTTSPATHATEHRAHARADEEDRPVHLTVTLDLPADPPTAMRML
                     ADPDYVHAKVRATGALDQQADVVEGENGAFTVTTRRSLPTDQIPANLRAFVGSSLHVR
                     QVEAWDTPRPDGGRRGTVVVEITGAPVRLTGTMVLAARGSTSTLTYDGDLKAGIPLFA
                     GAVESAAADAIRDALAVEGVVAARWLEGHGTGGEA"
     gene            830..2323
                     /locus_tag="VFC06_02500"
     CDS             830..2323
                     /locus_tag="VFC06_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HZL80803.1"
                     /translation="MSDLASRHASLDASDLEWLHLLVGDWQVVSDLAFADLVLWLPTT
                     DGDFIAIAQCRPSTGATVHYDDVVGSRPPDGQRPQLQRSLTEVRALRSREPRWFGSYA
                     VREEAVPVVHDGRAIAVIARQTNLGGARTPSRLELNYVEAADDLMGMIARGHFPAVNA
                     PTGPRRGAPRVGDGLIRLNSEGEVLYASPNALSCFHRLGAIGDLVGRSLVEVTTDVID
                     EPIAFDESMPLVLMGRAPWRTDLELHGVALSLRAVPLTEEGQRLGAVVLCRDVSELRR
                     RERELMTKDATIREIHHRVKNNLQTVAALLRLQSRRMSSPEARDALAEAMRRVATIAL
                     VHETLSQTLDEIVPFDDLVGRSLRLAADVASAGSTVRTVVHGTFGLVPAEDATALALV
                     LTELVTNAVEHGLSSRGGGTVDVTAEREGSNLKVTVADDGVGISAGAGAGTGLGTQIV
                     QTLVTNELRGTIDWGPREGGGTQVVLDIVLRNPGRDAATSGPESGGW"
BASE COUNT          319 a          860 c          877 g          353 t
ORIGIN      
        1 gtacggcact ggtcacgtgt caaggacggg ttcgtccgaa agggtgtggt agcgatccca
       61 tgggcacatc gttgcaggtc agagccctgc gcgccgcgag cgtgttatcg aattgttacg
      121 cctcgccacc cgttccgtga ccttcgagcc agcgtgccgc gacaaccccc tcgacggcga
      181 gcgcgtcgcg gatcgcgtcg gctgcggcag attcgacggc gccggcgaac agggggatgc
      241 cggccttgag atcgccgtcg tacgtcaggg tcgacgtcga gccgcgcgct gcgagcacca
      301 tcgtccccgt caggcgcacc ggggcccccg tgatctcgac gacgaccgtc ccacgccgtc
      361 cgccgtcggg ccgaggtgtg tcccaggcct cgacctgacg cacgtgcagg ctcgacccga
      421 cgaacgcgcg caggttggcc gggatctggt cggtcggcag cgaccggcgg gtcgtgacgg
      481 tgaacgcacc gttctcgcct tcgacgacgt cggcctgctg gtcgagcgcg ccggtcgccc
      541 ggaccttggc gtggacgtag tccggatcgg cgagcatgcg catcgcggtc ggtgggtcgg
      601 ccggtaggtc gagcgtgacg gtcaggtgca caggacggtc ctcctcatcg gcgcgtgcgt
      661 gcgcgcggtg ctcggtcgcg tgcgtcgcag gcgacgtggt caccggcccc gacgaacgtc
      721 gcaggtcgca ggcccttgcg cgcgcgcccg gcaccacgct actgcgccgt gggcgcgctc
      781 gccgcgagcc ggcgagccgg tgcgacctag gctggtcctc gtgcccaccc tgagcgacct
      841 cgcgtcgcgt catgcctcgc tcgatgcttc cgacctcgag tggttgcacc tgctcgtcgg
      901 ggactggcag gtcgtgtccg acctcgcgtt cgccgacctc gtgctctggc tgccgacaac
      961 cgacggcgac ttcatcgcca tcgcccagtg ccgccccagc acgggtgcga cggtccacta
     1021 cgacgacgtc gtgggctctc ggccgcccga cggtcagcgc ccgcagctgc agcgttcgct
     1081 caccgaggtg cgtgcgctgc gctcgcgcga accgcgctgg ttcggctcgt acgcggtgcg
     1141 cgaggaggcg gtccccgtcg tgcacgacgg acgcgcgatc gccgtcatcg cacggcagac
     1201 gaacctcggt ggagcccgca cgcccagccg gctcgagctc aactacgtcg aggccgccga
     1261 cgacctcatg ggcatgatcg ctcgagggca cttcccggcg gtcaatgccc ccaccggccc
     1321 gcgccggggt gcgccgcgcg tgggcgacgg cctcatccgg ctcaactcgg agggcgaggt
     1381 cctctacgcg agcccgaacg ccttgagctg cttccaccgg ctgggcgcga tcggcgacct
     1441 cgtcgggcgc tcgctcgtcg aggtgacgac cgacgtgatc gacgagccga tcgcgttcga
     1501 cgagtcgatg ccgctcgtgc tcatgggccg cgcgccgtgg cgcaccgatc tcgagctgca
     1561 cggggtcgcg ctgtcgctgc gcgccgtccc gctcaccgag gagggtcagc ggctcggtgc
     1621 cgtggtgctg tgccgcgacg tctcggagct ccgccgccgc gagcgcgagc tcatgacgaa
     1681 ggacgcgacc atccgggaga tccaccaccg ggtcaagaac aacctgcaga ccgtcgcggc
     1741 actcctgcgg ctccagtcgc gccgtatgag cagccccgag gcccgcgacg cgctggccga
     1801 ggccatgcga cgcgtcgcga cgatcgccct ggtccacgag acgttgtcgc agaccctcga
     1861 cgagatcgtg ccgttcgacg acctcgtggg tcgcagcctg cggctcgcgg ccgacgtcgc
     1921 gtctgcgggc agcacggtgc ggaccgtggt gcacggcacc ttcggtctcg tccccgcgga
     1981 ggacgcgacg gcgctcgcac tggtcctgac cgagctggtc acgaacgcgg tcgagcacgg
     2041 tctgtcgtcg cgcggcggcg ggacggtcga tgtcacggcc gaacgggagg gctccaacct
     2101 caaggtcacg gtggccgacg acggggtcgg gatctctgcg ggcgcgggcg ccgggaccgg
     2161 gctcggcacg cagatcgtgc agacgctcgt caccaacgag ctgcggggca cgatcgactg
     2221 gggaccgcgt gagggcggcg ggacgcaggt cgtgctcgac atcgtcctgc gcaacccggg
     2281 tcgcgacgct gcgacgtcgg ggccggagtc gggcggctgg tgacgcgggc cgggtttgcc
     2341 cggcctggag gcgccggctc acgacccacg gccgtcgcga cacgacaaag ccgccggcgc
     2401 tcgtggtga
//
LOCUS       DASRKH010000147         2052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_3982, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000147 DASRKH010000000
VERSION     DASRKH010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3982"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1532
                     /locus_tag="VFC06_02505"
     CDS             <1..1532
                     /locus_tag="VFC06_02505"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HZL80804.1"
                     /translation="LPIPVRDRRFWVIQALVLGIVVTHLALERDPFLAAESQFYLLSV
                     SILLVPVVYAGMRFGVSGALPTTAWALMLSAPEILGHRTPTRVGILVQFAIIFMIAFV
                     VGLRADRERAAAGATEQANARLSRINATASAVAQSLDLGRVLDETLRAKLDRSTRQVA
                     WIELLPGPNTPRVTVVAASQVVVPSELDEIQRGLTEAVCLSGQVQREPGRDQAARSAA
                     APIISDGIVVGALGVTYPSEPIPPDEYRVLEAVGTQLGTALSNIRHHASAREGLVALS
                     IANENLETYVELATEAQEEERKRLSRELHDDTMQSLVLTLAQIDTAMASELPDEAEAR
                     LADAKGILAETLDNIRRFCRDLRPSLIDDLGLVDAVDWLVSDLRSRVGMTVGLEVHEP
                     RRRLSSRDELLVFRIVQEALHNVERHAQAAWVGVVLDFRDGRLVAQVVDDGRGFASSG
                     RVEREKLDQGLGLRGMHERTKLLRGSLVVESRPGRGTTVRLSVPVAPAGRPVACSSLV
                     D"
     gene            complement(1583..1912)
                     /locus_tag="VFC06_02510"
     CDS             complement(1583..1912)
                     /locus_tag="VFC06_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80805.1"
                     /translation="MGFVDPGLLGGLRGRGAAGEPFVALVGWLVADAVRDLAPSRRAH
                     TRLDVVRVVSASTLVVFAIGVALWHARHPGDESGEAIIFAMASGVSGAMIATGAQVAT
                     GLAERGA"
BASE COUNT          328 a          696 c          712 g          316 t
ORIGIN      
        1 ggctgccgat ccccgtacgg gatcgccggt tctgggtgat ccaggcgctc gtgcttggca
       61 tcgtagtgac acatctcgcc ctggaacgcg accccttcct ggcggcggag tcccagttct
      121 acctgttgtc ggtgtcgata ctccttgtcc cagtggtgta cgccggcatg aggttcgggg
      181 tgagcggagc tcttccgacg acggcctggg cactgatgct ctctgcgccg gagatcctcg
      241 gacaccgcac accgacgcga gtggggatct tggtccagtt cgcgatcatc ttcatgatcg
      301 cgttcgtcgt cggtctgcgc gcggatcggg agcgcgcggc ggccggggcg accgagcagg
      361 ccaacgcacg gctgtcgcgg atcaatgcga ccgcgtcggc ggtggcacag tcgctcgacc
      421 tcggccgggt gcttgacgag acgctgcggg ccaagctcga tcgatccacg cgccaggtgg
      481 cgtggatcga gctgctgccg ggcccgaaca cgccgagggt cacggtcgtt gcggccagcc
      541 aggtggtggt gccgagtgag ctcgacgaga tccagcgggg gcttaccgag gcggtgtgtc
      601 tcagcggaca ggtgcagcgg gaaccgggtc gggatcaggc cgcaagaagc gccgccgccc
      661 cgatcatctc tgacgggatc gtcgtcgggg cgctgggagt gacgtacccg agcgagccga
      721 tccctcccga tgagtaccga gtgctggaag ccgtcggcac gcagctgggc acggcgctca
      781 gcaacatccg tcaccacgcg agcgcccgcg aggggctggt cgcgctgtcg atcgccaacg
      841 agaacctcga gacgtacgtc gagctggcca ccgaggctca ggaagaagag cgcaaacgcc
      901 tctcacgcga gctgcacgac gacacgatgc agtcccttgt cctcaccctt gcgcagatcg
      961 acacggccat ggcttcggag ctgcctgacg aggcggaggc tcggctcgca gatgcgaagg
     1021 gcatcctcgc cgagaccctg gacaacatcc gtcggttctg ccgggacctg cgcccctccc
     1081 tgatcgacga cctcggcctt gtcgacgcgg tcgactggct cgtgagtgat cttcggtcac
     1141 gggtgggcat gaccgtcggt ctcgaggtcc atgaaccgcg tcgccgcctc agcagccgag
     1201 atgagctcct ggtcttccgg atcgtgcagg aggccctgca caacgtggaa cgccatgccc
     1261 aggcggcatg ggtcggggtc gtgctcgact tccgcgacgg ccggctcgtg gcccaggtgg
     1321 tggacgacgg tcggggcttt gcctcgagcg gtcgtgtcga gcgcgagaag ctcgaccagg
     1381 ggctcgggct gcgtggcatg cacgagcgga ccaagctgct caggggatcg ctggttgtcg
     1441 agagccgacc cggtcgagga accacggtgc ggctgtctgt ccccgtcgca cccgcgggtc
     1501 gcccggttgc gtgcagctct ctcgtcgact gagtgcgccg tcgcgagacg gcagccgtgg
     1561 ccgccctacc ggaaggacgc ccctacgctc cgcgctccgc gaggcccgtc gcgacctgtg
     1621 cgcccgtcgc gatcatcgcg ccgctcaccc ccgacgccat cgcgaagatg atcgcctcgc
     1681 cgctctcgtc cccggggtgc cgcgcgtgcc agagtgcgac gccgatcgcg aagaccacga
     1741 gcgtcgacgc ggagacaacg cggacgacat cgagtcgcgt gtgcgcgcgg cgagacggcg
     1801 ccaggtcgcg cacggcgtcg gcgacgagcc acccgacgag cgcgacgaac ggttcgcccg
     1861 ccgccccgcg accccgaaga ccccccagca gtcctggatc aacgaacccc aaccagaact
     1921 acagagcacc tgaacaacga actgtctcac ttgacttgac agataccgag tccccgccca
     1981 gggcgagggt gacagcgagg tcggtgatga acttcgccgg gtcgtggatc gcgagcggcc
     2041 tgcgccaccg ac
//
LOCUS       DASRKH010000148         2095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_47507, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000148 DASRKH010000000
VERSION     DASRKH010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47507"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VFC06_02515"
     CDS             complement(<1..182)
                     /locus_tag="VFC06_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017200434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZL80806.1"
                     /translation="MNLEVDVDDGRPIFVQIAEQIEGQIVDGTMPEESQVPSINELAA
                     FHRINPATALKGVNVLV"
     gene            276..881
                     /gene="orn"
                     /locus_tag="VFC06_02520"
     CDS             276..881
                     /gene="orn"
                     /locus_tag="VFC06_02520"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020013235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HZL80807.1"
                     /translation="MPDAPLVWIDCEMTGLDLATDALIEVACIVTDADLNPLGEGVSV
                     IIKPPSDAVEVMTDFVRQMHVKSGLLPELDGGLTMEDAQRQLVDYVTAQAKGFRAPLA
                     GNTVGMDKAFLDRDMPELMGLLHYRVVDVSSIKELVRRWYPRVFFNSPDKTGGHRALG
                     DIEDSIQELRYYREAIFSPQPGPDSVAAKAAAAAVIEAQSD"
     gene            969..1044
                     /locus_tag="VFC06_02525"
     tRNA            969..1044
                     /locus_tag="VFC06_02525"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1002..1004,aa:His,seq:gtg)
     gene            complement(1096..1353)
                     /locus_tag="VFC06_02530"
     CDS             complement(1096..1353)
                     /locus_tag="VFC06_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012903125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HZL80808.1"
                     /translation="MEILWALVVGAVLAVIASLLQRGKSGMPWWGNWIAGIGGAVIGF
                     WLAAQLGVAATPGVDWIRWIISIIVAVILLGAGGRFFAKKS"
     gene            1448..>2095
                     /locus_tag="VFC06_02535"
     CDS             1448..>2095
                     /locus_tag="VFC06_02535"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HZL80809.1"
                     /translation="MIAALTGAGLSAAAGLNAFIPLVLVGIFARFTTFIELPENLMWL
                     QEWPTIIIALLLLAVELVLDKIPGVDHVNDLIQTAVRPLVGGVIFAASASAAELDVGQ
                     FWTDNPMIAGIVGAVITLVVHTGKSASRPAVNASTGGTGAPLASFAEDATSVALSLLA
                     IFAPILVTVVFFGLGFAFYYIITSGRRRRRNREILQAQERLEREAAHERGEKVAWW"
BASE COUNT          365 a          700 c          642 g          388 t
ORIGIN      
        1 accagcacat tcactccttt gagtgccgtc gccggattga tccggtgaaa cgcggcgagc
       61 tcattaatcg atggcacttg agactcctcg ggcatggttc cgtcgacgat ctgtccttcg
      121 atctgttccg cgatctggac aaagatgggg cgaccatcgt ccacgtcgac ctccaggttc
      181 atgggttagt tggtgatgta actcaccaac ccgcacggtg tggatttcca acccgggcac
      241 catccccgca gcaggttggc cctacagtgg gagtcatgcc cgatgcaccc ttggtctgga
      301 tcgactgcga aatgacgggt ctcgatctcg ccaccgacgc ccttatcgaa gtggcctgca
      361 tcgtgaccga tgcggacttg aacccccttg gcgagggcgt gtccgtcatc atcaagccgc
      421 cctccgacgc ggtcgaggtc atgacggact ttgtgcgcca gatgcacgtg aagtccggcc
      481 tgttgccgga acttgacggc ggcctcacga tggaggacgc ccagcgacag ctggtcgact
      541 acgtcaccgc gcaggccaag gggttccgcg cgccgcttgc gggcaacacc gttggcatgg
      601 ataaggcgtt cctcgaccgc gacatgccgg aactgatggg gttgctgcac taccgcgtcg
      661 tggacgtcag ttcgatcaag gaactggtgc gtcgctggta cccccgggtg ttcttcaact
      721 cgcccgataa gacgggtggc caccgcgcgc tcggcgacat cgaggactcc atccaggagc
      781 ttcggtacta cagggaagcg atcttctccc ctcagccggg gccggactcg gtcgccgcca
      841 aggccgcggc cgccgccgtc atcgaggctc agtccgactg atcgaggtcg gaaccttcac
      901 gcgaacatgc gcgcgtgcac cacggcacgc gcctcgcggt agactttccg ctggccctcg
      961 ggccgatggt ggctatagct cagttggtag agcaccgcgt tgtggtcgcg ggggccgcgg
     1021 gttcaagtcc cgttagccac cccacgacga agcccccccg ctcacgcggg ggccttctct
     1081 cgtttgcggc tgccgctaac tcttctttgc gaagaatcgg ccgccagcgc ccagcaagat
     1141 cacggccacg atgatcgaga tgatccagcg gatccagtcg acgcccgggg tcgcagcgac
     1201 gcccagttgc gcggcgagcc aaaatccaat cactgcgccg ccgatgcccg cgatccagtt
     1261 gccccaccac ggcatacctg actttccccg ctgcaacaac gaggcgatga cagccagcac
     1321 ggcgcccacg accaatgccc aaagaatctc catgatgaca acctttcgac tcgagtcggg
     1381 ccagtcccga ccccccaaca ggctatcgtg agggcgtgat tgacgtagga acacccggga
     1441 ggcacgagtg atcgccgcac tgaccggcgc aggcctgtct gcggcagcgg ggctcaatgc
     1501 gttcatcccc ctcgtgttgg tgggcatctt cgctcgcttc accactttca ttgagttgcc
     1561 cgagaacctc atgtggctac aggagtggcc cacgatcatc atcgcgctgc tccttctcgc
     1621 cgtggagttg gttctcgaca agatccccgg tgtcgaccac gtcaacgacc tcatccagac
     1681 ggcggtgcgc ccgctcgtcg gaggagtgat cttcgcggcg agcgcctctg ccgcagagtt
     1741 ggacgtcggc cagttctgga cggacaaccc gatgatcgcc ggcatcgtcg gcgcggtgat
     1801 caccctcgtc gtccacaccg gcaagtctgc gtcgcgcccg gctgtcaacg cctcgaccgg
     1861 aggcacgggt gcaccactgg cgtccttcgc cgaggatgcc acgtcggtgg cgttgagcct
     1921 gctcgcgatc ttcgcgccga tcctcgtcac cgtggtgttc tttgggctcg ggttcgcgtt
     1981 ctactacatc atcacgtcgg gacgccgccg gcggcgcaat cgcgagatcc tgcaagcaca
     2041 ggagcggctc gagcgcgaag cggcgcacga acgaggcgag aaggtcgctt ggtgg
//
LOCUS       DASRKH010000149         2588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_41343, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000149 DASRKH010000000
VERSION     DASRKH010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41343"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2057)
                     /locus_tag="VFC06_02540"
     CDS             complement(<1..2057)
                     /locus_tag="VFC06_02540"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HZL80810.1"
                     /translation="MSTTANKAPATTDLVTVMIDGIETQVEKGTLIIRAAEQVGIEIP
                     RFCDHPALKPAGACRQCLVDVASPAGPEGKLRAFPKPQTSCTTPVTEGMVVNTQLTSE
                     EAERAQKGVMELLLINHPLDCPVCDKGGECPLQNQSLSHGYGESRFVDVKRVFEKPLV
                     LSSEILLDRERCIMCQRCTRFSQEIAGDAFIDLQNRGAMQQIGTFNEDVLGFGGYGPV
                     GAAAEDESGKAFSSYYSGNTIQICPVGALTSAAYRFRSRPFDLVSSPGISEHDSSGSA
                     IRTDHRRGKILRRLAQDDPVANEYWITDKDRFAFTWQNQADRITQPMVRKDGELVEVS
                     WPEALAAAAEGLTAAAGKTGTSADKKAKGVGVLPGGRITMEDAYAYGKFARLVLDTND
                     VDSRTRISSEEEQSFLGASVAGTGLGVTFAELEKAPTVLLVGFEPEDEGGVVFLRLKK
                     SVGHQNVITVGAYRSRGAEKLGATLLPAVPGHEATAVAALKGSKAAYKQLREPGSIII
                     VGERAAGSAGTLTAVVALAKRAKAKVAWIPRRAGERGAVEAGALPGLLPGGRDVTVAA
                     GRDAVARVWGASSLPRTKGRDADAIIAAVGAKALDALVVGGVDDSDLTEDLLKAIDKA
                     KFTVSLEVRRTAVVDAADVVLPVAPPSEKSGTFVNWEGRLRDFDTAIRTDAMSDHRVL
                     DLLA"
     gene            complement(2054..>2588)
                     /locus_tag="VFC06_02545"
     CDS             complement(2054..>2588)
                     /locus_tag="VFC06_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HZL80811.1"
                     /translation="CELLEMAGGMRDGHELKFWTPGGSSTPIFTQDHLDTPLDYESVG
                     AAGSMLGTRALQIFDETTCVVRAVERWTEFYKHESCGKCTPCREGTYWLALILHRLEA
                     GQGTMADIDQLLGTCDSIAGRAFCALGDGATSPVISSVTLFREEYERHVTDGGCPFDR
                     AASALFDYDSAPEGAHA"
BASE COUNT          405 a          872 c          870 g          441 t
ORIGIN      
        1 gcgagaaggt cgagcacacg gtggtcagac atcgcgtcgg tgcgaatcgc ggtgtcgaag
       61 tctcggagtc gaccctccca gttgacgaac gttcccgact tctcgctggg cggcgcgacg
      121 ggcaacacca catcagcggc gtcgactacc gcggtgcgac gcacctcgag gctcaccgtg
      181 aacttcgcct tgtcgatcgc cttcaacagg tcttccgtaa ggtcgctgtc gtcaacaccg
      241 ccgacaacga gcgcgtcgag tgccttcgcg cccacggcgg cgatgatcgc gtcggcgtcg
      301 cggcccttgg tgcgcggcag cgacgacgcc ccccacaccc tggccacggc atcccggccc
      361 gcggcaacgg tcacgtcgcg gccccccggg agcaggcccg gaagggcgcc cgcctcgacg
      421 gccccgcgtt cgcctgcgcg ccgcggaatc caggcaacct tggccttcgc gcgcttcgcg
      481 agcgccacca cggcggtgag ggtgcccgcc gaaccagcgg ctcgctcacc cacgatgatg
      541 atcgaaccgg gctcgcgaag ctgcttatag gcggccttgg agcccttgag cgcggccacg
      601 gcagtggcct catggcctgg cacggctggc agcagcgtcg cgcccaactt ctccgcaccg
      661 cggctgcggt atgcgccgac cgtgatgacg ttctggtgtc ccacagactt cttgaggcgc
      721 aggaacacca cgccgccctc atcctcgggc tcgaagccca ccagcaagac ggtgggcgcc
      781 ttttccagtt cggcgaacgt gacgcccaat ccggtgccgg caacgctggc gcccaggaag
      841 gactgctcct cttccgacga gatgcgggtg cgcgagtcca cgtcgttggt gtcgagcacc
      901 agccgcgcga acttgccgta ggcgtaggca tcttccatcg tgatgcggcc gccgggcagg
      961 acgcccacac ccttggcctt cttgtcggcg gacgtgccgg tcttgcctgc tgcagccgtg
     1021 agaccctcgg cggcggcggc gagcgcctcc ggccaggaca cctcgaccag ttcgccgtcc
     1081 ttacggacca ttggctgggt gatgcggtcg gcctggttct gccacgtgaa cgcgaagcga
     1141 tccttgtcgg tgatccagta ctcgtttgcc accgggtcgt cctgggccag gcggcgcaga
     1201 atcttgccgc gacggtggtc ggtacggatc gcgcttccgg agctgtcgtg ctccgagatg
     1261 ccggggctgg acaccaggtc gaacgggcgc gagcggaaac gatacgccgc gctcgtgagt
     1321 gcgcccacgg ggcaaatctg gatggtgttg cccgagtagt acgagctgaa ggctttgccc
     1381 gactcgtcct cggcggcggc gcccacgggg ccgtagccgc caaagcccag cacgtcctcg
     1441 ttgaacgtgc cgatctgctg catggcgccg cggttctgca agtcgatgaa cgcgtcgccg
     1501 gcgatctctt gagagaagcg ggtgcagcgc tggcacatga tgcaccgctc gcggtcgagc
     1561 aggatctcgc tgctgagcac gaggggcttc tcgaagacgc gcttgacgtc cacgaagcgg
     1621 ctctcaccat agccgtggct cagggattgg ttctgtaggg ggcactcgcc gcccttgtcg
     1681 cacaccgggc agtccagtgg gtggttgatg agcagcagct ccatcacgcc cttctgtgcc
     1741 cgttctgcct cttccgacgt gagctgggtg ttgacgacca tgccttcggt caccggtgtg
     1801 gtgcacgagg tctgcggctt ggggaaggcg cgcaacttac cctcggggcc agcggggctg
     1861 gccacgtcca cgaggcattg gcggcacgcg ccagccggct tcagagccgg gtggtcgcag
     1921 aagcgcggaa tctcgatacc gacctgttcg gcggcacgga tgatgagcgt gcccttctcc
     1981 acttgcgtct cgatgccgtc gatcatgacg gtcaccaggt cggtcgtggc gggagccttg
     2041 ttcgcggtcg tgctcatgcg tgggccccct cgggtgccga gtcgtagtcg aacagggccg
     2101 atgcggcgcg gtcgaacggg cagccgccgt cggtcacgtg gcgctcgtat tcctcgcgga
     2161 acagcgtgac ggagctgatg acaggcgacg tggcaccatc accaagcgcg cagaacgcgc
     2221 ggccggcaat cgagtcgcac gtgcccaaca gctgatcgat gtcggccatg gtgccctgac
     2281 ctgcctcaag cctgtgcagg atcaacgcaa gccaatacgt gccctcgcga cacggggtgc
     2341 acttgccgca ggattcgtgc ttgtagaact cggtccagcg ctccacggcg cgcaccacgc
     2401 acgtagtctc gtcaaagatc tgcagggccc gggtgccgag catcgacccg gccgcgccca
     2461 ccgactcgta gtcgaggggg gtgtccaggt gatcttgagt gaagatcgga gtcgaggatc
     2521 cgccgggggt ccagaacttc agttcgtggc cgtcccgcat gccaccggcc atctcgagca
     2581 gttcgcac
//
LOCUS       DASRKH010000150         3742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_12669, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000150 DASRKH010000000
VERSION     DASRKH010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12669"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VFC06_02550"
     CDS             complement(<1..323)
                     /locus_tag="VFC06_02550"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HZL80812.1"
                     /translation="MNPLSIPSPPVSVWQLGPFPLRAYAMAILLGIAVATVILQRRYE
                     RRGGPGERVLDLVLWMVPFGIIGGRVYHVISSPDAYFGPGGDPWKVFAIWNGGLGIWG
                     AIALGG"
     gene            complement(320..1117)
                     /gene="trpA"
                     /locus_tag="VFC06_02555"
     CDS             complement(320..1117)
                     /gene="trpA"
                     /locus_tag="VFC06_02555"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HZL80813.1"
                     /translation="MSSQSEVALDRRTQSGGAGFIGYLTVGYPDLETSIRAGRALIDA
                     GVDIIELGLPYSDPGMDGPVIQYTGQLALEAGTRTRHVMRAVQELAGSVPILVMTYFN
                     PVHRYGVEGFARDLASAGGAGLITPDLIPDEAAAWIAAATQHDLDRVFLVAPSSTDER
                     LRLTADACRGFVYAASTMGVTGERLRVDDTAEVLVARTRAVGAERVCVGLGVSNAAHA
                     REVGSYADGVIVGSALLRALGDGVASMRHLAVELADAAHASRLPGRP"
     gene            complement(1114..2337)
                     /gene="trpB"
                     /locus_tag="VFC06_02560"
     CDS             complement(1114..2337)
                     /gene="trpB"
                     /locus_tag="VFC06_02560"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HZL80814.1"
                     /translation="MPAQSLGSLSGPYFGDFGGRFVPEALIAALDDLDAAWTRLKDDP
                     AFIAELAELHRTYTGRPSILTDAKRFSALAGGARILLKREDLNHTGSHKINNVLGQAL
                     LTRAIGKHRVIAETGAGQHGVATATAAALLGLECTVYMGAEDVRRQALNVARMRLLGA
                     EVIPVVAGSRTLKDAINEAFRDWVTNVGTTNYIFGTAAGPHPFPTIVRDLQKIIGEEA
                     REQVLALTGRLPDVVMACVGGGSNAIGIFNAFLDDPGVRLIGLEAAGEGIETGRHAAS
                     IAGGEPGVLHGARSYLLQDEDGQTRESHSISAGLDYPGVGPEHSYLATIGRAEYWPVT
                     DAEAMDAFAALCHTEGIIPAIESAHALAGAIRLGNQAGEAAKDLVILVNLSGRGDKDV
                     STAAEWFDLLQEGQA"
     gene            complement(2347..3174)
                     /gene="trpC"
                     /locus_tag="VFC06_02565"
     CDS             complement(2347..3174)
                     /gene="trpC"
                     /locus_tag="VFC06_02565"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015771499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HZL80815.1"
                     /translation="MSILDDIIAGVREDLAARQALVSLDELRERASRAPSAKDGVAAL
                     RNPSGAVAIISEVKRSSPSKGALSQILDPAALAVQYELGGASAISVLTEQRRFGGSLS
                     DMDAVRAAVDVPVLRKDFVVSPYQIWEARAHGADLVLLIVAALEQKVLTSLVERVHSL
                     GMTALVEAHDRLEAMRAVNAGARVVGINARNLKTLEVRRDVFSSVVEVVPSSVIKVAE
                     SGVRGPHDVIEYARAGANAVLVGEALVTHANPRQAVAEMVAAANHPALSQRDPASGN"
     gene            complement(3189..3449)
                     /locus_tag="VFC06_02570"
     CDS             complement(3189..3449)
                     /locus_tag="VFC06_02570"
                     /inference="COORDINATES: protein motif:HMM:NF041681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HGxxPAAW family protein"
                     /protein_id="HZL80816.1"
                     /translation="MTDEGYTLPPAAAPRNHGHTTAAWALVAGVVLGTLVLGIGIALG
                     HTTLAVIGGAIVAASLLLSLAMRSRGLGQPLGEKKPRDWYAD"
     gene            complement(3446..>3742)
                     /locus_tag="VFC06_02575"
     CDS             complement(3446..>3742)
                     /locus_tag="VFC06_02575"
                     /inference="COORDINATES: protein motif:HMM:NF021079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trp biosynthesis-associated membrane protein"
                     /protein_id="HZL80817.1"
                     /translation="AALEAAAADVTGVPASAGEVGVHPVAWVAVTGLVVATIVALYAV
                     RAAGSWPRDRSRFERAPQAVAIDPRTRAMDDWDALGRGEDPSGIDGTLDPKEDR"
BASE COUNT          528 a         1321 c         1361 g          532 t
ORIGIN      
        1 ccgcccagcg cgatggcgcc ccagatcccg agtccgccgt tccagatggc gaacaccttc
       61 catgggtcgc cgcccggccc gaagtacgcg tcgggggagg agatcacgtg gtagacacgg
      121 ccgccgatga tgccgaacgg caccatccac agcacgaggt cgaggacgcg ctccccggga
      181 cccccgcgcc gctcgtaccg ccgctgcagg atcaccgttg ccaccgcgat accgagcagg
      241 atggccatcg cgtaggcgcg cagcgggaag ggtccgagct gccacacgga gaccgggggg
      301 ctggggatgg acagcgggtt cacgggcgcc ccggcagccg tgacgcgtgg gccgcgtcag
      361 ccagttcgac cgcgaggtgg cgcatcgagg cgacgccgtc cccgagtgcc cgcaggagtg
      421 cggaccccac gatgacgccg tcggcgtagg agccgacctc gcgggcgtgg gccgcgttcg
      481 acacgcccag gcccacgcag acgcgttcgg cgccgacggc gcgggtgcgg gccacgagga
      541 cttcggccgt gtcgtccacc cggaggcgct ccccggtcac ccccatggtg gaggcggcat
      601 acacgaagcc gcggcaggcg tcggccgtga ggcgcagccg ctcgtcggtc gacgacggcg
      661 cgaccaggaa cacccggtcg aggtcgtgct gtgtggcggc ggcgatccag gcggcggcct
      721 catccgggat gaggtccggc gtgatgagtc ccgccccgcc ggcggaggcg aggtcccggg
      781 cgaacccctc caccccgtag cggtgcacgg ggttgaagta cgtcatgacg aggatgggca
      841 ccgagcccgc cagctcctgc accgcccgca tcacgtgccg ggtgcgggta cccgcctcga
      901 gggcgagttg gccggtgtac tggatgacgg ggccgtccat gccgggatcg gagtacggca
      961 gtccgagttc gatgatgtcc acgcccgcgt cgatgagcgc cctgcccgcc cggatcgacg
     1021 tctcgaggtc cgggtacccc acggtcaggt acccgatgaa gccggcgccg ccggactgcg
     1081 tgcggcggtc gagtgccacc tcggactgcg aactcatgcc tggccctcct gcagcaggtc
     1141 gaaccactcg gcggcggtgg acacgtcctt gtcgccacgg cccgagaggt tgacgaggat
     1201 gaccagatcc ttcgccgcct ctcccgcctg gtttccgagc cggatggccc cggcgagcgc
     1261 gtgggcggac tcgatcgcgg ggatgatccc ctcggtgtgg cacaacgccg cgaacgcgtc
     1321 cattgcctcg gcgtcggtga ccggccagta ctccgcccgc ccgatggtgg cgaggtacga
     1381 gtgctcgggt ccgacgcccg ggtagtcgag tcccgcggag atggagtggg actcgcgggt
     1441 ctgcccgtcc tcgtcctgca gcaggtagga tcgcgcaccg tgcaggacgc cgggttcgcc
     1501 cccggcgatc gaggccgcgt ggcggcccgt ctcgatgccc tcgcccgccg cctccagccc
     1561 gatgaggcgc acccccgggt cgtccaggaa ggcgttgaag attccgatgg cgttcgagcc
     1621 gccaccgacg cacgccatga cgacgtcggg gagccggccg gtgagtgcga gcacctgctc
     1681 gcgggcctcc tcgccgatga tcttctgcag gtcgcggacg atcgtgggga agggatgcgg
     1741 acccgccgcg gtcccgaaga tgtagttggt ggtcccgacg ttggtcaccc agtcgcggaa
     1801 cgcctcgttg atggcgtcct tcagtgtcct ggacccggcc accaccggga tgacctcggc
     1861 ccccaggagc cgcatgcgag cgacgttgag cgcctgccgg cgcacgtcct ccgcacccat
     1921 gtagacggtg cactccagcc cgaggagtgc tgccgcggtg gcggtcgcga cgccgtgctg
     1981 gccggccccg gtctcggcga tcacgcggtg cttcccgatc gcgcgggtga ggagtgcctg
     2041 cccgagcacg ttgttgatct tgtgcgagcc ggtgtggttg aggtcctcgc gcttcagcag
     2101 gatgcgggca ccgcccgcga gggcggagaa gcgcttggcg tccgtgagga tggagggacg
     2161 gccggtgtag gtgcggtgca gctctgccag ttcggcaatg aacgccgggt cgtccttgag
     2221 cctcgtccag gcggcgtcga ggtcgtcgag cgcggctatg agggcctcgg ggacgaaacg
     2281 cccgccgaaa tcgccgaagt acggtccgct gagcgacccg agggactgtg ctggcatgtt
     2341 tccttcctaa ttcccgctgg ccgggtctcg ctggctcagc gcggggtggt tggcggcggc
     2401 caccatctcg gccacggcct ggcgggggtt cgcgtgggtc acgagcgcct cccccacgag
     2461 cacggcatta gcgccggccc tggcgtattc gatcacgtcg tgcgggcccc gcacgccgga
     2521 ctccgccacc ttgatcacgg agctgggcac cacctccacc acggacgaga acacgtcgcg
     2581 gcgcacctca agggtcttca ggttgcgggc gttgatgccc accacgcggg cgcccgcgtt
     2641 gaccgcgcgc atcgcctcga gccggtcatg ggcctcgacc agcgccgtca tcccgagcga
     2701 gtgcacgcgc tccaccagcg acgtgagcac cttctgctcc agcgcggcga cgatgaggag
     2761 gacgaggtcc gcgccatggg cgcgtgcctc ccagatctgg taggggctca ccacgaagtc
     2821 cttgcgcagc acggggacgt cgaccgcggc ccggacggcg tccatgtcgg acagcgaacc
     2881 accgaaacgc cgctgttccg tcaggacgga gatcgccgag gccccgccga gttcgtactg
     2941 gaccgcgagc gcggcagggt cgaggatctg ggacagcgct cccttggacg ggctggaccg
     3001 cttcacctcg gagatgatgg ccacggcgcc ggaggggttg cgcaacgccg cgacgccgtc
     3061 cttggcggag ggcgcccgtg aggcgcgctc cctcagctcg tcgaggctga cgagcgcctg
     3121 ccgtgcggcc aggtcctccc ggacgcctgc gatgatgtca tccagtatgg acatggtctc
     3181 gcccgcgcct agtcggcgta ccagtcgcgc ggcttcttct ctccgagtgg ctggccgagg
     3241 cccctggacc tcatcgccag cgagaggagg agcgaggccg ccacgatcgc gccgccgatg
     3301 accgccagtg tggtgtgccc gagggcgatg ccgatcccga gcacgagggt gcccaggacg
     3361 acgcccgcga ccagtgccca tgcggccgtc gtgtggccgt ggttccgcgg cgcagccgcc
     3421 ggcggcagtg tgtagccctc gtcagtcatc tgtcctcctt ggggtccaga gtaccgtcaa
     3481 tccccgatgg gtcctcgccg cggccgaggg cgtcccagtc gtccatcgcg cgggtccggg
     3541 ggtcgatcgc gaccgcctgg ggggcccgtt cgaaccggga cctgtcgcgc ggccaggagc
     3601 ccgcggcccg caccgcgtac agcgccacga tcgtggcgac gacgagcccc gtgacggcca
     3661 cccacgcgac cgggtggacg ccgacctccc ccgcggaggc gggaacgccg gtgacgtcgg
     3721 ccgcggccgc ctcaagcgcg gc
//
LOCUS       DASRKH010000151         2015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_4578, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000151 DASRKH010000000
VERSION     DASRKH010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4578"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VFC06_02580"
     CDS             <1..242
                     /locus_tag="VFC06_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZL80818.1"
                     /translation="VGALSELAGDRRSPVLVLVTHHVEEIPPGFTHLLLLRAGRVHAA
                     GAIDETLTAENLSGAFGLDLVVDRVGDRWTARASR"
     gene            357..815
                     /locus_tag="VFC06_02585"
     CDS             357..815
                     /locus_tag="VFC06_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HZL80819.1"
                     /translation="MSGWLWWVGGALLLGIAEIVSVQLVFLMFAGGALAGALASVLGA
                     TEPVQIGVAAVTSMLLLFALRPWLLRRLRRRVPLVETNAAALVAREAVVLSTTTEDGG
                     RVKLGGEVWSARAAHGGEVFAPGAEVHVVRIEGATAVVSAVGGSARTPHS"
     gene            887..>2015
                     /locus_tag="VFC06_02590"
     CDS             887..>2015
                     /locus_tag="VFC06_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015771463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HZL80820.1"
                     /translation="MDQPGYIILALVILFVVIVLVRAVRIVPQATALIIERLGRYSRT
                     LDAGLHLLIPFVDRVRAGVDLREQVVSFPPQPVITSDNLVVSIDTVIYFQVTEPKSAV
                     YEIFNYIIGIEQLTVTTLRNVIGSMDLEQTLTSRDQINGQLRGVLDEATGKWGIRVNR
                     VELKSIDPPASIQGSMEQQMRAERDRRAAILTAEGVKQSQILTAEGDKQGAILRAEGS
                     AQAAILTAEGEARAILQVFDAVHRGDADPKLLAYQYLQILPKLAASPANKVWIIPSEL
                     TGALSQLTQGFAGSFTGQPDGTSAPARPVGASPLGADLPPVVLTSPAEALAEARRQSA
                     LATADATSAGTLSGRPFDPSAELGQRPGAAGGVPSHRSGRPA"
BASE COUNT          278 a          707 c          722 g          308 t
ORIGIN      
        1 tcgtcggcgc gctctccgag ctggcggggg accgccggtc gccggtgctc gtgctcgtca
       61 cgcaccacgt cgaggagatc cccccgggct tcacccacct gctgctgctg cgtgcgggtc
      121 gcgtgcacgc cgccggggcc atcgacgaga cgctgaccgc ggagaacctg tccggggcct
      181 tcggtctcga cctcgtcgtg gaccgcgtcg gcgaccgctg gaccgcacgc gcgtcccgat
      241 agcgggaaag gtcgcccaag acctcacgga aaggcaaagc gaccacctag gatcggttgt
      301 gacggccgta ctgcgcatcg atgcgacggt gcgcccgccg ggacctggag gtcgacatga
      361 gcggttggct gtggtgggtg ggcggcgcgc tgctgctcgg gatcgccgag atcgtgtcgg
      421 tccagctggt cttcctgatg ttcgccggcg gagcgctcgc cggggccctc gcgagcgtgc
      481 tgggcgccac cgagccggtg cagatcggcg tcgccgcggt gacgtccatg ctgttgctct
      541 tcgcgctccg gccatggctc ctgcgtcgcc tgcgtcgccg ggtcccgctc gtcgagacga
      601 acgccgcggc gctcgtcgcg cgcgaggccg tggtcctgtc gaccacgacc gaggacggtg
      661 gccgcgtgaa gctcgggggc gaggtctggt cggcgcgggc cgcgcacggg ggcgaggtct
      721 tcgcgcccgg ggccgaggtt cacgtcgtcc ggatcgaggg cgcgacagcc gtcgtgagcg
      781 ccgtcggcgg ctctgcccgg acgcctcaca gctgattgcg cggtcgacct gaccgcgccc
      841 gaactgccgt gcgagagtcc tcggacggtc acaccgagga gaacacatgg atcaacccgg
      901 gtacatcatc ctggcgctcg tcatcctctt cgtcgtcatc gtgctggtgc gtgcggtgcg
      961 gatcgtcccg caggccaccg cgctcatcat cgagcggctg gggcggtact cgcgcacgct
     1021 cgacgccggc ctgcacctgc tcatcccgtt cgtcgaccgg gtccgcgcgg gcgtcgacct
     1081 gcgtgagcag gtcgtgtcgt tccccccgca gcccgtgatc acgtccgaca acctcgtcgt
     1141 gagcatcgac accgtcatct acttccaggt gacggaaccg aagtcggcgg tctacgagat
     1201 cttcaactac atcatcggta tcgagcagct gacggtcacc acgctgcgca acgtcatcgg
     1261 gtcgatggac ctcgagcaga cgctgaccag ccgcgaccag atcaacggcc agctgcgcgg
     1321 tgtgctcgac gaggcgaccg gcaagtgggg catccgggtg aaccgggtcg agctcaagtc
     1381 gatcgacccg cccgcaagca tccagggctc gatggagcag cagatgcgtg ccgagcgcga
     1441 ccgtcgtgcc gcgatcctca cggccgaggg cgtcaagcag tcgcagatcc tcacggctga
     1501 gggtgacaag cagggtgcga tcctgcgggc cgaggggtcc gcccaggcgg cgatcctcac
     1561 ggccgagggt gaggcccgcg cgatcctgca ggtcttcgat gcggtgcacc gtggtgacgc
     1621 cgacccgaag ctgctcgcgt accagtacct gcagatcctg cccaagctcg cggccagccc
     1681 ggcgaacaag gtctggatca tcccgtccga gctcaccggt gccctgtccc agctcacgca
     1741 ggggttcgcg gggtcgttca ccggtcagcc ggacggcacg tccgcgccgg cacgtccggt
     1801 gggcgcctca ccgctcggtg cggacctgcc gccggtcgtg ctgaccagcc cggccgaggc
     1861 gctggccgag gcacggcggc agtccgccct ggcgacggct gacgcgacga gcgcggggac
     1921 gttgagcgga cgtccgttcg acccgtccgc cgagctcggc cagcgacccg gagcagcggg
     1981 tggcgtgccg tcgcaccggt cggggcgtcc ggctc
//
LOCUS       DASRKH010000152         2351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_54486, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000152 DASRKH010000000
VERSION     DASRKH010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54486"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VFC06_02595"
     CDS             <1..510
                     /locus_tag="VFC06_02595"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HZL80821.1"
                     /translation="GLIHGVQTNIHRRDVAWADHDEATQSTNAGSDERTPPAPERRRQ
                     GGPVSRIEMSHEEFEDLVRDAMDTLPEWTVPILEELAVLVEDAPKPGTTRPGTTLLGL
                     YRGIPVTAHGGRVPGSMPGTITLYRLPLLAVCASREDVPARVLKVLGHEVGHAMGISE
                     SELRELGWF"
     gene            571..>2351
                     /locus_tag="VFC06_02600"
     CDS             571..>2351
                     /locus_tag="VFC06_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HZL80822.1"
                     /translation="MAQSRPTRSRTASSKSTGRAPARKNPRSRAPARKQPKYAWPVRA
                     LKGIGSGSARMVGGGVRRMGQGARDVPHEVRRDGIALVLIIAAILVSASEWFQLDNWF
                     GHVMHRIAAGTFGVMALVLPVLLVAMAIRLFRTPQEGDANYRIVFGSVLLALAVGGII
                     HIANDAPSPSDGLVPVQEAGGILGFLIGTPLAQLLTPPLAITVLALLAIAFTLFVIGK
                     PLREVVAGAVGLVRGLWSGSDDDADLDLPEHGTLEPKKRSSLLKKAADARKRRLDGGY
                     EADEAFASAATRERRDFENMVHPGSAARHEDTSTREDATVPTPMHGSPRHGTGAHGIV
                     SPYAPSGDPELDAMDAADADSKLTTKIERTAPHTSVVPAGEQGKLENSNPYVLPALEV
                     LAKGAPHKTRSSANDRVVASLTSVLDQFGVDAVVTGFMRGPTVTRYEVELGQGVKVEK
                     ITQLSKNIAYAVASPDIRILSPIPGKMAIGIEIPNTDRETVSLGDVLRSPVAFKNDHP
                     MAIGIGKDVEGGYVMANLAKMPHLLVAGATGAGKSSFVNSMITSILMRATPQEVRMVL
                     VDPKRVELSIYEGIPHLITPIITDPKKA"
BASE COUNT          385 a          837 c          768 g          361 t
ORIGIN      
        1 ggtctcatcc atggggttca aactaacatt caccgccggg acgtagcctg ggcagatcat
       61 gatgaagcga cgcagtccac caacgcgggg agcgacgagc gcacgccgcc cgcgcctgag
      121 cgcaggcgcc agggaggacc agtgagtcgc atcgagatgt ctcacgagga gttcgaagac
      181 ctcgtcaggg acgccatgga tacgcttccg gagtggaccg tcccgattct tgaggagctc
      241 gcggtgctcg tggaggacgc gcccaagccc ggcaccacgc gcccagggac caccctgctg
      301 ggcctgtatc ggggcattcc cgtgacggcc catggcggtc gcgtgcccgg ctcgatgccc
      361 ggcaccatca cgctgtatcg gctgcccctc ctggcggtgt gtgcgagccg cgaggacgtt
      421 cctgcacgcg tgctcaaggt gctcggccac gaggtcggcc acgccatggg aatctcggaa
      481 tctgaactgc gcgaactcgg ctggttctaa gcgcgcttcc cggggcgcct cctgaccggc
      541 gtctgcctcg cgtcgtagcc tgggggcact atggctcagt ctcgccccac gcgctcacgc
      601 accgcctcgt ccaagtcgac ggggcgcgct cccgcgcgca agaaccctcg cagcagggcc
      661 ccggcccgca agcagccgaa gtacgcgtgg ccggtgcgcg ccctcaaggg catcggctcg
      721 ggctctgccc gcatggtggg cgggggagtg cgacgcatgg gtcagggcgc tcgcgacgtc
      781 ccgcacgagg tacgccgcga cgggatcgcg ctggtcctga tcatcgcggc catcctggtg
      841 tctgcgagcg agtggttcca gctcgacaat tggtttggcc acgtgatgca ccgcatcgcc
      901 gccggcacgt tcggcgtgat ggcgcttgtg cttccggtgt tgctggtcgc catggccatt
      961 cgcctgttcc gcacgcccca agagggcgac gcgaactacc ggatcgtgtt cggctccgtg
     1021 ctgctggcgt tggccgtggg cggcatcatc cacatcgcga acgacgcccc atccccgtcc
     1081 gatggcttgg ttcccgtgca agaggccggc ggcatcctcg gtttcctcat cggcacccca
     1141 ctcgcgcaac tgctgacgcc gccactcgcc atcaccgtgc tcgctttgct ggcgatcgcc
     1201 ttcaccctgt tcgtgatcgg caagcctctg cgtgaggttg tcgcgggcgc tgtgggcctg
     1261 gtgcgcggcc tgtggtccgg cagcgacgac gacgccgacc tcgacctgcc ggagcacggc
     1321 acgcttgagc ccaagaagcg cagcagcctg ctcaagaagg ccgccgatgc gcgcaagcga
     1381 cgcctcgacg gcggctacga ggccgacgag gccttcgcct cggcggccac ccgcgagcgc
     1441 agagacttcg agaacatggt gcaccccggc agcgccgcga gacacgagga cacctctacc
     1501 cgcgaggatg cgacggtccc cacgccgatg cacggctcac cgcggcacgg cacgggcgcc
     1561 cacggcatcg tctccccgta cgcgccctcc ggcgatccgg agctggacgc catggacgcg
     1621 gccgatgccg actccaagct caccaccaag atcgagcgca ccgcgccgca cacctccgtg
     1681 gtgccggccg gggagcaggg caagttggag aattccaacc cttacgtgct gcccgcgctc
     1741 gaggtgttgg ccaagggcgc accccacaag acccgctcga gcgccaacga ccgcgtggtc
     1801 gcgtcgctca cgagcgtgct cgaccagttc ggggtggacg cggtcgtcac gggcttcatg
     1861 cgcggcccca cggtcacgcg ctacgaggtg gagctgggcc agggcgtcaa ggtcgagaag
     1921 atcacccagc tgtccaagaa cattgcctac gcggtggcaa gccccgacat ccggatcctg
     1981 tcgcccatcc cgggcaagat ggcgatcggc atcgagatcc cgaacacgga ccgcgagact
     2041 gtctcccttg gcgacgtgtt gcgctcgccg gtggcgttca agaacgacca ccccatggcg
     2101 atcggcatcg gcaaggacgt cgagggcggc tacgtgatgg ccaacctcgc caagatgccc
     2161 cacctcctgg tcgcgggcgc cacgggcgcc ggcaagtcat cctttgtgaa ctcgatgatc
     2221 acgtccatcc tgatgcgcgc cacgccccaa gaggtgcgca tggtgctcgt ggaccccaag
     2281 cgcgtcgaac tgagcatcta cgagggcatc ccccacctga tcacgcccat catcaccgac
     2341 cccaagaagg c
//
LOCUS       DASRKH010000153         2700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_48577, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000153 DASRKH010000000
VERSION     DASRKH010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48577"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(258..1454)
                     /locus_tag="VFC06_02605"
     CDS             complement(258..1454)
                     /locus_tag="VFC06_02605"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HZL80823.1"
                     /translation="MDDDGIETDAWAADQGPAADALLPASAAWRDGDNPGRRQFLSLG
                     DLPLEADPMGVLPGATLAYETWGQLNAARDNAIYVAHALTGDSHVWGPSEPGHHTGGW
                     WNAMVGPGRPIDPERHFVVSANVLGGCQGSSGPASAHPADGKPWGSRFPYVTLRDMVA
                     AEIALADHLGVDRWRLITGPSMGGMRAIEWAVTVPERVGAIAPVGSTAATTADQIAWS
                     TSQVAAILADPKFRGGDYYDAADGDGPHVGLGIARMIAHTTYRSEGELADRFGRSFQG
                     DGDPIGKGGLFAVQSYLEHHGRKLARRFDANTYVRLAQAIQSHDVGRGRGGVETALAR
                     YTGPALVIAVDSDRLYPLKNSQLLDLGLSGSQGVKVVHSPVGHDGFLVESGQLNAFIA
                     EFEAEL"
     gene            1630..>2700
                     /locus_tag="VFC06_02610"
     CDS             1630..>2700
                     /locus_tag="VFC06_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80824.1"
                     /translation="MTYRLFDRWTHPVVLACVIALIAAAVVASSPRAAGADTYPGLSD
                     IAAAKASVADASATVAELDAAVVKLEQARTDAENAALLAADVYSTAKGDAERSQRQSV
                     AASARADDAVAALDNARSDLGVIAMEAYRSGGSMTSVEAILGADGFDDVIARSEDYGR
                     AAAEVDSVVQRVRATEVVAGTMSDFAADAAQQAADAADAAAVALTKAEDSRRVAEQAV
                     ADAQATREDAVVRLAQLQNTTVQLEQQRQAGLASERQRVQRARLAAALTAAENAAAEK
                     VAAEKAAAEQTAATQPPPAAAPPATTPPATTPPATTPPATTPPAPTPPPVDPSGSWSS
                     SASQGQSAAVFAQTLLGSPYELG"
BASE COUNT          433 a          962 c          907 g          398 t
ORIGIN      
        1 ctgcgagttc tgcgacttga ccggtgagcg caaagatgcc cgcgccaacc atgacgccgg
       61 tgcccatcgc caccgcgccg cgcagggaga gtgatccctc tcgatagtta tctgccatat
      121 gccaggttcg gcgtgcgtcg tgcgcaggat attcccgcgc acgccgccgc cgtcaccgcc
      181 atcgtagcgt cggccacggc gccgagagag cgaagagtcc gagggaaagg cagacccagg
      241 tgggcaaact gtccttgcta cagctcggcc tcaaactccg cgatgaacgc attgagctgc
      301 cctgactcca ccaggaaccc gtcgtgaccc acgggcgagt gcaccacctt cacgccctgt
      361 gagccggaca agccgaggtc gaggagttgc gaatttttga gcgggtagag ccggtcggag
      421 tccaccgcga tcaccagggc cggcccggtg taacgcgcca gcgccgtctc gacgccgccg
      481 cggcctcgcc ccacatcgtg cgactggatc gcctgcgcca gccgcacgta cgtgttggca
      541 tcgaagcggc gcgcgagctt gcgcccgtgg tgttccaggt acgactgcac cgcgaacagc
      601 ccgcccttgc cgatcgggtc gccgtctccc tggaacgagc gcccgaagcg atcggccagt
      661 tcgccctcgg agcggtacgt cgtgtgcgcg atcatgcgcg cgatgccgag ccccacatgg
      721 ggtccgtcgc catcggccgc gtcgtagtag tcgccgcccc ggaacttggg gtcagccaag
      781 atcgcggcga cttgagaggt ggaccacgcg atctggtcag ccgtggtggc cgcagtcgag
      841 cccaccggcg cgatggcccc gacgcgctcg ggtacggtca cggcccattc gatggcgcgc
      901 atgccgccca tggacggacc cgtgatgagc cgccaccgat ccacgcccag gtgatcggcc
      961 aacgcgatct ccgccgccac catgtcgcgc aacgtcacgt aggggaagcg cgagccccac
     1021 ggcttgccgt cggcagggtg agcggacgcc ggcccggagg agccctgaca gccgccgagc
     1081 acgttcgccg agaccacgaa gtgacgctcg ggatcgatgg gtcgccccgg gccgaccatg
     1141 gcgttccacc agccacccgt gtggtgtccg ggctcggaag gcccccacac gtggctgtcc
     1201 cccgtgaggg cgtgcgccac gtagatcgcg ttgtcgcgtg cggcgttgag ttgtccccag
     1261 gtctcgtacg cgagggtcgc cccaggcagc acccccatgg ggtcggcctc aaggggaagg
     1321 tcgcctagcg acaggaattg gcggcgcccg ggattgtctc cgtcgcgcca cgccgcagag
     1381 gcggggagga gcgcgtcggc cgccggaccc tggtccgccg cccacgcatc tgtttcgatc
     1441 ccgtcgtcat ccatgccgac accaccctag ggcaggacgc cgccgcgtgg agagtcgcga
     1501 gtcggcgggc tcaagtccga cagccatgcc gccgatagaa gatacgcgcg tggatattgc
     1561 cacatcaccc ttccgcgcct tgcgagcgcg gcctacgctg gccaaacatc gggcaaagga
     1621 gtgcgaaagg tgacataccg gctgtttgac cgctggaccc accccgtggt cttggcctgc
     1681 gtgatcgcgc tgatcgccgc ggccgtggtg gccagttcgc cgcgcgccgc aggagccgac
     1741 acctaccccg gcctttcaga cattgctgcg gccaaggcct ccgttgccga tgcctccgcg
     1801 actgtcgccg aactggacgc cgccgtggtg aagttggagc aggctcgcac cgatgccgag
     1861 aatgccgcac ttctggccgc cgacgtctac tcgacagcca agggcgacgc ggaacgttcc
     1921 cagcgccagt ccgtcgctgc ttccgcgagg gcggacgatg ctgttgcggc gttggacaat
     1981 gcgcgcagcg atctcggcgt catcgccatg gaggcctacc gttcgggcgg ttccatgacc
     2041 tctgtcgagg cgatcctcgg tgccgacggc tttgacgacg tcattgcccg ctccgaggac
     2101 tacggccgtg ccgctgcgga ggtcgattcc gtcgttcagc gggtacgcgc caccgaggta
     2161 gtcgcgggaa ccatgagcga cttcgccgcg gacgcggctc agcaagccgc cgacgccgcc
     2221 gatgccgccg ccgtggcgct taccaaggcc gaggactcgc ggcgcgtggc cgagcaggcc
     2281 gttgccgacg cccaagcgac tcgcgaagac gcggttgttc gattggctca gctccagaac
     2341 accaccgttc agcttgaaca gcagcgccag gcggggctcg cctccgagcg acagcgcgta
     2401 caacgggcgc ggttggcggc ggcgctaacg gccgccgaga atgccgcagc ggagaaggtg
     2461 gcggcggaga aggcggcggc ggaacaaacc gcagctacgc agccacctcc cgcagcggcg
     2521 cctcccgcga cgacaccgcc cgcgaccaca cctcctgcga ccacgcctcc cgcgaccacg
     2581 cctcctgcgc cgacgccgcc cccggtggat ccctccggat catggtcgtc gagcgcatcg
     2641 cagggtcagt cggccgccgt gttcgcccag acgttgctgg gctcgccgta tgagttgggc
//
LOCUS       DASRKH010000154         2953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_835, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000154 DASRKH010000000
VERSION     DASRKH010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_835"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..1467)
                     /locus_tag="VFC06_02615"
     CDS             complement(10..1467)
                     /locus_tag="VFC06_02615"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80825.1"
                     /translation="MGAHRPRRRSRRGRLVIARVVVDSPLPHLDRPFDYAIGEPLEAV
                     VRVGSRVRVPFAGRLSSAVVVELADTESPHALKSIKSAGAVPSFTQQALDLASSIAQR
                     YGGSLWDVLRLMAPARVASVEKLDWKSMEAVHLDPELLAGEVGRAAEHGLPTVQGSRS
                     AWVAPPATSATAPVEALLAWALAAVADGGSAIVAVPDARAAQAVAARAVESGMRRWTA
                     RSGGHFVLLDADDGPSVRFGSYLAAMHGLARLVIGTRSAAWQPVPDLKAIAIWDEGST
                     TFAEPRAPYPQARTVAAMRAQDTGASFLVAGYALSPDAVALVEHGFARRVGGAPEREA
                     LPRIEIIGPERREREGGQGKHWMPGSVWTPLLGAAQDGVAGILVPQSGYASGLRCARC
                     SAWAECADCGGDLQRSSVEGTPRCRECALEAAHWHCPECQGYRLTPAGLGVERLADQV
                     ARMAPGVPLAVSSSGTGIVADGAVTSGLVVATPAA"
     gene            complement(1422..2585)
                     /gene="metK"
                     /locus_tag="VFC06_02620"
     CDS             complement(1422..2585)
                     /gene="metK"
                     /locus_tag="VFC06_02620"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HZL80826.1"
                     /translation="MSLRLFTSESVTEGHPDKVCDQVSDAILDEMLRQDPKSRVAVET
                     MVTTGLVHVAGEVTTEAYVDIPSTIRSVVRGIGYTSSSVGFDADSCGVSVSIGEQSPD
                     IWLGVGQATDTGVIELGAGDQGLMFGYACKDTAELMPLPIAMAHALTFRLAQVRKEGI
                     VEGLRPDGKAQVTIGYDGDTPISVDTVVVSTQHREGWNIDDLREEVEAGVIAPVLARY
                     DLDSSGHRSLINPTGRFEIGGPMGDAGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVD
                     RSAAYAMRWVAKNIVAAGLADRCEIQVAYAIGTAMPVGLYVETFNTHTVDPDRIIKAV
                     REVFDLRPSALIAQLDLRRPIFRSTASYGHFGRELPDFTWERTDRADALAAAV"
     gene            complement(2645..>2953)
                     /locus_tag="VFC06_02625"
     CDS             complement(2645..>2953)
                     /locus_tag="VFC06_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HZL80827.1"
                     /translation="VETDDILRSLVESKAPHQLVVGFAAETGDDAGSVLDHGRAKALR
                     KGADLLVVNPVGASAGFGDVPNSVTILDAAGDVVATSDGTKMVVAHAILDQVAARLPR
                     "
BASE COUNT          501 a         1025 c          976 g          451 t
ORIGIN      
        1 ggctggcggc tacgcggctg gcgtggcaac gacaagcccc gaggtgacgg caccgtccgc
       61 gacgatgccc gtgccggacg aggagaccgc gagcggtacg ccgggcgcca tcctcgcgac
      121 ttggtcggcg agtcgctcca ctccgaggcc tgcgggcgtg agccggtagc cctggcattc
      181 ggggcaatgc caatgggccg cctcgagcgc gcactcgcgg cagcgcggcg tgccctcgac
      241 cgatgatcgc tgcaggtcgc caccacagtc ggcgcactcc gcccacgcgg agcatcgcgc
      301 gcagcgaagg ccgctcgcgt agcccgactg gggcacgagg atgcccgcca cgccgtcttg
      361 agcagcccca aggagggggg tccacacgga gccaggcatc caatgcttgc cttgaccccc
      421 ttcgcgctcc cgtcgctcgg ggccgatgat ctcgatgcgg ggtagggcct cgcgttcggg
      481 cgcaccaccc actctccgcg cgaagccgtg ttcgacgagc gccaccgcgt ccggcgacag
      541 cgcgtagccg gccacaagga acgacgctcc ggtatcttgg gcgcgcatcg ccgcgacggt
      601 gcgggcctgc gggtatgggg cgcgcggttc ggcgaacgtg gtcgatcctt catcccagat
      661 ggcgatcgcc ttgaggtcgg gaaccggttg ccacgcggct gacctcgtac cgatcaccag
      721 cctggccaat ccgtgcatcg ccgccaggta cgaaccgaat cgcaccgacg ggccatcgtc
      781 cgcgtcgagc aacacgaaat gcccgcccga acgcgcggtc caccggcgca tccccgactc
      841 gactgcccgg gcggcgacgg cctgggctgc gcgagcatca ggaaccgcca cgattgcgga
      901 gcccccgtcc gcgacggcgg cgagcgccca cgcgagcaac gcctcgaccg gggccgtggc
      961 cgatgttgcg ggcggggcga cccatgcgga acgactcccc tgcaccgtcg gcaatccatg
     1021 ctcagcggct ctgcccacct caccagccag cagttccgga tccaggtgaa cggcctccat
     1081 ggacttccag tcgagcttct ccaccgaggc cacgcgcgcg ggcgccatga gccgcagcac
     1141 gtcccacaac gaacccccat accgctgcgc tattgacgac gccaggtcca gcgcctgctg
     1201 agtgaacgac ggcaccgcgc cagccgactt gatcgacttg agtgcatgag gtgattcggt
     1261 atcggcaagc tccacgacca cggcggagct caagcgaccc gcgaacggga ccctcactcg
     1321 gctacccacg cggacgacgg cttcgagtgg ctcgccaatg gcgtagtcga aaggcctgtc
     1381 gaggtgcggt agcgggctat cgacgacgac ccgcgcgatc actagacggc cgcggcgaga
     1441 gcgtcggcgc ggtcggtgcg ctcccacgtg aaatcgggca attcccgccc aaagtgcccg
     1501 tagctggcgg tcgagcggaa gatcggccga cgcagatcaa gctgcgcgat gagcgctgac
     1561 gggcgcaggt cgaacacctc tcgcaccgcc ttgatgatgc gatccggatc cacggtgtgc
     1621 gtgttgaacg tctcgacgta caggcccaca ggcattgccg tgccgatcgc atacgcgacc
     1681 tggatctcgc agcggtctgc cagcccagca gcgacgatgt tcttggccac ccacctcatg
     1741 gcgtacgcgg ccgagcggtc caccttcgac gggtccttgc cggagaaagc gccgccaccg
     1801 tgtcgcgcca tgccgccgta cgtgtcgacg atgatcttgc gcccggtcag gccagcgtcg
     1861 cccatggggc cgccgatttc aaaacggccg gtggggttga tgagcgagcg gtggccggag
     1921 gagtcaaggt cgtagcgcgc gaggacgggt gcgatcactc cggcctcgac ctcttcgcgg
     1981 agatcgtcga tgttccagcc ttcgcggtgc tgggtggaca ccaccacggt gtcgaccgaa
     2041 atcggggtgt cgccgtcata cccgatagtg acctgggcct tgccgtcggg ccggaggccc
     2101 tcgacgatcc cttccttacg cacctgagcg agccggaatg tcagggcatg cgccatcgcg
     2161 atgggcagcg gcatcaactc ggcggtgtcc ttgcacgcgt aaccgaacat caggccctgg
     2221 tcgccggcgc ccagttcgat cacgccggta tcggtcgcct gtcccacgcc cagccagatg
     2281 tcggggctct gctcgccgat cgacacggag actccgcacg aatccgcgtc aaagccgacg
     2341 ctcgaggacg tgtagccgat gcccctcacg accgatcgga tggtcgaggg aatgtcgacg
     2401 taggcctcgg tcgtgacctc gccggcgaca tggactagac ctgtcgtcac catagtttcg
     2461 accgcgaccc gtgacttggg atcctggcgc agcatctcat cgagaatggc gtcggagacc
     2521 tggtcacaga ccttgtcggg gtgtccctcg gtcacagatt cggacgtgaa gaggcgcaga
     2581 gacatagcgc tcagcatact tgccggatcc gacatcgggc cgcgccgtga ggcggccggg
     2641 tccgctatcg cgggagccgc gcagccacct ggtcgaggat ggcgtgagcg acgaccattt
     2701 tggtgccatc gctcgtggcg accacgtcgc cagccgcgtc cagaatcgtt accgaattgg
     2761 ggacgtcgcc gaagccggca ctcgcgccta cgggattcac gaccagcaga tccgctccct
     2821 ttcgcaaggc cttggcgcgt ccatgatcga gaacgctgcc cgcgtcatca ccggtctcgg
     2881 cggcgaaacc cacgacgagt tggtggggag cctttgactc tacgagagac ctcaggatgt
     2941 cgtcggtctc tac
//
LOCUS       DASRKH010000155         2041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_13066, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000155 DASRKH010000000
VERSION     DASRKH010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13066"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VFC06_02630"
     CDS             complement(<1..342)
                     /locus_tag="VFC06_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZL80828.1"
                     /translation="MSEPAPRLPSARVDPVRTTWPHQLSHAPTLRVGDVLAALRADFP
                     SLTSSKLRFLDAQELVAPSRAPSGYRQYSHADVERVRYVLRQQRDAYAPLGVIKERLD
                     TLDSGLAHEPLS"
     gene            complement(351..740)
                     /locus_tag="VFC06_02635"
     CDS             complement(351..740)
                     /locus_tag="VFC06_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZL80829.1"
                     /translation="MSLGSHVPADEAAPVRLSAQDQVAVDALAEDHALLVVHHGPNAG
                     ARFLLDVDLTTAGRSVSCDIFLDDVTVSREHAHFVRVGNTFLMRDAGSLNGTYVNRER
                     FAEAELHEGDEVQIGKYRLTFYPGHRH"
     gene            complement(797..1384)
                     /locus_tag="VFC06_02640"
     CDS             complement(797..1384)
                     /locus_tag="VFC06_02640"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HZL80830.1"
                     /translation="MPPAPLRSDSWWRLNATIPNAITVARIVLIVVFVVLLGRHEDAW
                     AITALAVAGVSDFLDGYLARRLEQTSALGRVLDPAADRILTVAVVIGLAWRDIVPWWL
                     VAILLARDAVMAVALVWLARRRLATPTVTFLGKSATAALYVFLPLTYLAYERWDAVHT
                     VALWGAVAAAVAYWGSAAQYLSQARRAGSHDVSTP"
     gene            complement(1419..1565)
                     /locus_tag="VFC06_02645"
     CDS             complement(1419..1565)
                     /locus_tag="VFC06_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80831.1"
                     /translation="MSDDTTPSRHEVYEQIRANELARGASEDDAARVAAEKVDQVLEL
                     KRAE"
     gene            complement(1679..>2041)
                     /locus_tag="VFC06_02650"
     CDS             complement(1679..>2041)
                     /locus_tag="VFC06_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZL80832.1"
                     /translation="AARERGVEATWIVADLTAWVADEPADLVTVSFLHEPAHVRHAVW
                     RAAASAVAGGGTLLITGHRPDVPGASGPPPETRFTADEVIAALGAPWRAHVSEAQRDG
                     VGHHAGHVMTDLIITLTR"
BASE COUNT          364 a          693 c          648 g          336 t
ORIGIN      
        1 tgacagcggc tcgtgggcaa ggccggagtc cagcgtgtcc agtcgttcct tgatcacccc
       61 gagtggcgca tatgcgtctc gctgttgacg caagacatag cgaacccgct caacgtccgc
      121 gtgggagtac tgcctgtacc ccgacggggc ccgactgggt gccaccaact cttgagcgtc
      181 caaaaagcgg agcttggagg aagtgagcga cggaaagtcg gcgcgcagcg ctgccaagac
      241 gtctcccacc cgcaacgtgg gcgcatgcga caactggtgc ggccacgtcg tgcggaccgg
      301 gtcaaccctt gccgacggca atcgcggcgc cggttcgctc atcacgacgc ctagtgccgg
      361 tgacccgggt agaacgtcaa gcggtacttt ccgatctgta cctcgtcacc ctcatgcaac
      421 tcggcctcag cgaaccgttc gcggttgacg taggttccgt tcagggagcc cgcgtcgcgc
      481 atcaggaacg tgttgcccac tcgcacgaag tgagcgtgct ctcggctcac cgtgacatcg
      541 tcgagaaaga tgtcgcatga gaccgaacga cccgcagtgg tgaggtcgac gtcaaggagg
      601 aatcgagccc ccgcgttggg gccgtggtgc accacgagca aggcgtgatc ttcagccagc
      661 gcgtcaaccg ccacctggtc ctgagcgctg aggcgcacag gggctgcctc atcggcgggc
      721 acatgggagc cgagtgacat cgtcctctcg accggcgact cgtcgtatga catggcgcct
      781 cctttcatcg accattctag ggtgtagaca cgtcatggga accggcccgc ctcgcctggg
      841 aaaggtattg cgcagcggat ccccagtacg ccaccgcagc ggccacggct ccccacagcg
      901 cgacggtgtg cacggcgtcc caccgctcgt acgccaggta ggtcaaaggc agaaatacgt
      961 acagcgcggc cgtggccgac ttgcccagaa acgtcacggt gggagttgcc agccgtcgcc
     1021 gtgcgagcca caccagcgcg acggccatca cggcgtccct ggccagcaag attgcgacca
     1081 gccaccacgg gacgatgtca cgccaggcga ggccgatgac gacggcgacc gtgaggattc
     1141 gatccgcggc aggatccagc acgcgaccga gcgctgatgt ctgttccaaa cgcctcgcga
     1201 gatagccatc gaggaagtcg ctcacgcccg cgacggcgag ggcggtaatg gcccacgcgt
     1261 cctcgtggcg ccccaagagc acgacaaaca cgacaatgag tacgatgcgc gccaccgtga
     1321 tcgcgttggg aatggtcgcg ttcaggcgcc accacgagtc ggaacgaagt ggtgcgggcg
     1381 gcatggactc atcgtagggg cccgtacccc ccgaagaact attcggccct cttcaactcc
     1441 aggacctggt cgaccttctc cgcggcgacg cgcgcggcat catcttcgga agctcctcgc
     1501 gccagctcgt ttgcgcgaat ctgttcgtaa acctcgtgcc gactgggtgt ggtgtcgtct
     1561 gacatggtgc ctcccttcgt ggctacttcg aacgtacccc gctggggcat ttgccgccac
     1621 actccgcggc cggtttcgtg aagtagccgc gcacgtcggc gaggggcctc tcggtgccct
     1681 accgcgtgag cgtgatgatc agatccgtca tcacgtggcc ggcatggtga ccgacgccgt
     1741 cccgttgcgc ctcgctcacg tgagctcgcc atggcgcgcc aagtgcggcg attacctcgt
     1801 ccgcagtgaa gcgcgtctcg ggcggtggcc cggaggcgcc tggtacgtct ggccggtgac
     1861 ccgtgatcaa gagcgttccc cctccagcca ccgcgcttgc cgcggctcgc cacaccgcat
     1921 gacgcacgtg tgcaggctcg tgcaggaagg acaccgtcac caggtccgcg ggctcatcgg
     1981 ccacccatgc ggtcaagtcc gcgacaatcc aggtggcctc cactcccctc tcccgcgcgg
     2041 c
//
LOCUS       DASRKH010000156         2105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_5310, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000156 DASRKH010000000
VERSION     DASRKH010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5310"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /gene="rapZ"
                     /locus_tag="VFC06_02655"
     CDS             complement(<1..913)
                     /gene="rapZ"
                     /locus_tag="VFC06_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HZL80833.1"
                     /translation="MADPTDTPGTPTVPQGIPMRDEQTPLPAGERPEILIITGMSGAG
                     RTRAAAALEDLNWYVVDNLPPRLLMTLAAMMRTGDGISRLAAVVDVRSREFFTEMVSV
                     IGQIRQTGMDYRVIFLEASDGELVRRFDTVRRPHPLQGDGTVLDGIAEERKLLQSLKN
                     RADVIIDTSALSVHDLARKIRELVDDPTEKRLHLTVMSFGFKYGLPLDADHVVDVRFL
                     SNPYWVSELRHLTGHDKEVRSYVLALPGATEFIDRYVAAIEPVLDGYVDELKPYLTIA
                     VGCTGGKHRSVAISEAIAERLRRAGHTV"
     gene            complement(964..>2105)
                     /gene="uvrC"
                     /locus_tag="VFC06_02660"
     CDS             complement(964..>2105)
                     /gene="uvrC"
                     /locus_tag="VFC06_02660"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HZL80834.1"
                     /translation="DLQASVQVFHVRQGRIRGQRGWVTDRIDDASDGELIERLLVQVY
                     GAHTEAGGEAPRAIPREVLVPHLPDDVEALASWLRGARGGAVSVRVPRRGDKRALMDT
                     VHENARQALAAEKLRRGADLATRSRALEELQRFLVLADPPLRIECYDISHTGGTLQVG
                     SMVVFEDGVPKKRDYRVFNVRGADGTGARDDTEALAEVLTRRFERGRRELTAPRSGEV
                     AEPEPVLERESFAYDPGLLVIDGGLPQVNAAHRTLGELGVDVPVVALAKRLEEVWVPG
                     AEFPVILPRGSEALYLLQRLRDEAHRFAITRHRKRRSAAMTASVLDTVPGIGPGRAKA
                     LLKHFGSLKQLRGADVEEVGRVPGIGPALAHVVVEHFAAERTAAH"
BASE COUNT          306 a          739 c          739 g          321 t
ORIGIN      
        1 ggacggtgtg ccccgcccgc cgtaggcgct ccgcgatcgc ctcggatatc gccacggagc
       61 ggtgcttccc gccggtgcac ccgacggcta tggtcagata gggcttcagt tcgtcgacgt
      121 agccgtcgag caccggttcg atcgcggcga catagcggtc gatgaactcc gtggcgccgg
      181 ggagggcgag gacgtaggaa cggacctcct tgtcatgacc cgtcaggtgc cgcaattcgc
      241 tcacccagta gggattcgag aggaaccgga cgtccaccac gtggtcggcg tccagcggga
      301 ggccgtactt gaagccgaac gacatcacgg tgaggtgcag gcgcttctcc gttgggtcgt
      361 ccaccagttc gcggatcttg cgggccaggt cgtggacgga gagcgcggac gtgtcgataa
      421 tgacgtctgc ccggttcttc agcgactgga ggagcttgcg ctcctcggcg atgccgtcca
      481 ggaccgtgcc gtcgccctgg agcgggtggg gacggcgcac ggtgtcaaag cgtcgcacca
      541 gctccccgtc ggatgcctcg aggaagatga cccggtagtc catgccggtc tgccggatct
      601 ggccgatcac ggagaccatc tcggtgaaga actcccggga ccggacgtcc acgaccgccg
      661 cgaggcgaga gatcccgtcc cccgtccgca tcatggcggc aagcgtcatg agcaaccggg
      721 gcgggaggtt gtcgaccacg taccagttga ggtcctcgag cgccgccgcc gcgcgggtac
      781 ggccggcgcc cgacattccc gtgatgatca ggatttccgg tcgctcaccg gcggggagcg
      841 gcgtctgctc gtcccgcatc gggatccctt ggggaacagt cggtgtgccc ggtgtgtcag
      901 tgggatcagc catgctccaa gcatgccacg gcctctcgcg gccgcgacgg gtctcggccg
      961 ggctcagtgg gcggcggtgc gctccgccgc gaagtgctcc acgaccacgt gcgcgagtgc
     1021 agggccgatc ccgggcacgc ggcccacctc ctccacgtcc gcgccgcgca actgcttgag
     1081 ggagccgaaa tgcttgagga gggccttcgc cctgcccggg ccgatgccgg gcacggtgtc
     1141 cagcacggag gccgtcatgg cggctgacct gcgcttacgg tgacgcgtga tcgcgaaccg
     1201 atgcgcctcg tcgcgcagac gttgcagcag gtagagggcc tcgctaccgc gcggcagtat
     1261 gacggggaac tccgctccgg gcacccacac ctcctcgagg cgcttcgcga gtgcgaccac
     1321 ggggacgtcg acgccgagtt cgccgagcgt ccggtgggcg gcattcacct ggggcagccc
     1381 gccgtcgatc accaggagtc ccggatcgta ggcgaacgac tcgcgttcca ggaccggttc
     1441 cggctccgcc acctccccgg acctgggtgc ggtgagctcg cgccgtcccc gttcgaagcg
     1501 gcgtgtgagc acctccgcaa gcgcctcggt gtcgtcgcgt gccccggtgc cgtcggcgcc
     1561 ccggacgttg aacacccggt agtcccgctt cttcgggacg ccgtcctcga acaccaccat
     1621 cgagcccacc tggagcgtcc cgcccgtgtg cgagatgtcg tagcactcga tgcggagggg
     1681 tgggtccgcg agcaccagga accgctgcag ctcctcgagg gcgcgcgaac gggtcgcgag
     1741 gtcggccccg cggcgcagtt tctccgccgc gagggcctgc cgggcgttct cgtggaccgt
     1801 gtccatgagt gcacgcttgt cgccccgccg cgggacacgc accgacaccg cgccgccccg
     1861 cgcaccgcgc agccacgagg ccagcgcctc gacgtcgtcg ggcaggtgcg gcacgaggac
     1921 ctcccgcggg atggcgcgcg gcgcctcgcc acccgcctcg gtgtgggcac cgtacacctg
     1981 cacgaggagc cgctcgatga gctcgccatc gctcgcgtcg tcgatgcggt cggtgaccca
     2041 cccgcgctgt ccgcggatgc gaccctgccg cacatggaac acctggacgg acgcctggag
     2101 gtcgt
//
LOCUS       DASRKH010000157         2795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_14550, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000157 DASRKH010000000
VERSION     DASRKH010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14550"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..383)
                     /locus_tag="VFC06_02665"
     CDS             complement(33..383)
                     /locus_tag="VFC06_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80835.1"
                     /translation="MATRQKRRDFGSVRQVQPSGRWQASYLNPQDKSRRINAPTTYAK
                     EDRAEAWLSSERSLIEKGIWTSPLERAAASVTVEVFGREWLDEHRVRESTRVLYSDRL
                     EDHVFPHLVLQSRL"
     gene            complement(764..1936)
                     /locus_tag="VFC06_02670"
     CDS             complement(764..1936)
                     /locus_tag="VFC06_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013348199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1249 family transposase"
                     /protein_id="HZL80836.1"
                     /translation="MTGAGSGHSGSCVLCGTRLKRNGVTSAGTTRWRCPKCGSSITRR
                     RPDVTRAADLDRFLAWLMGRATQRDVDGRTGRRFRDQHAWCWNVAPTIAVTGEIYDEI
                     QLDGTYLSDGWCLLVAIDGATGQVIAWQWCDTEKTAAWTALLERIPPPRVVVVDGGSG
                     LASALKACWPNTRIQRCLVHVQRGVRTYLTTRPRTDAGRSLRVLSLALTRIRTIEEAG
                     RWQVNLNAWHQAYGPLINTRTYLKQVDIRPAWARPHATWWWTHDRLRKAYQLLARLTR
                     QGVLFTYLDPNLEALVIGSTTNRIEGGTNHPIKDLLRRHRGMTSDHQRRAVEWWCYLH
                     SPDPRAPRDLIKPEHYAPVPARQIKSEEPDGPAAYDTVTTAEEGLWTRTGWAGRSR"
     gene            complement(2120..>2795)
                     /locus_tag="VFC06_02675"
     CDS             complement(2120..>2795)
                     /locus_tag="VFC06_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007540329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZL80837.1"
                     /translation="DMSEYMEKHTVSRLIGAPPGYVGYEEGGQLTEAVRRRPYAVVLF
                     DEIEKAHPEVFNSLLQILDDGRLTDGHGRTVNFTNTIIVMTSNLGSDIVLAWDGADRS
                     VLEAQLHEVLKRAFRPEFLNRLDDVVVFDRIDRTAMEGIVDAELAKAVARIEQAKGIT
                     LTVSPALRASLAKDGFDPQFGARPLKRLIQTRLLNELAKDIVSGHLTEGQHVTAGWDG
                     TTVELT"
BASE COUNT          502 a          850 c          880 g          563 t
ORIGIN      
        1 aacaatgtca ctacaaagcc cgcggccaca ctctacaaac gcgactgtag tactaggtgt
       61 gggaagacgt ggtcttcgag gcggtcactg tagagaacgc gtgtcgactc ccggaccctg
      121 tgttcatcga gccactcgcg cccgaagacc tcgacggtga ccgaggcggc cgcacgctcg
      181 agcgggcttg tccagattcc cttctcaatc aggctcctct cgctgctgag ccacgcctcg
      241 gcgcggtcct ccttcgcgta agttgtgggg gcgttgatac ggcgactctt gtcttgtggg
      301 ttgaggtaac tcgcttgcca gcgtcctgac ggctgaacct gtcggacgct cccgaagtcg
      361 cggcgctttt gtcgtgtagc catggtggca ctccttaaca gttgactatt tcgaatctac
      421 cgcggaagtg gggcccgaat ttctgtcaca tgcaatctgt atgcaataag gggtgtctcc
      481 aaatggccat agatgtccac agttgtccac gacgggttca aacaaagtac ctggtaatag
      541 cacattcgcc gcgctggatc gccttggact cccaatgtaa gaagaattac cgcaagctgc
      601 ttcggaagac tcgccaccag cgtcgcaata agaagtagac gccaacaacg ctaagtctgt
      661 aaacaacgct gagcccgcgc ccgttagggg tgcgggcttt gtgggttagc ggcggaaatg
      721 tgtgtctggc cccggcgtgt cgcggctgcc ctggggtggt ggtttagcgt gagcgtcctg
      781 cccatccggt gcgggtccat aggccttcct cggctgttgt gacggtgtca taggccgcgg
      841 ggccgtcggg ctcctcggat ttaatctgtc gagcggggac aggggcgtag tgctctggtt
      901 tgatgaggtc tcgtggggct cgcggatcag gtgagtgcag atagcaccac cactcgactg
      961 cgcggcgttg gtggtcgctg gtcatgccgc ggtggcgacg caggaggtct ttaatggggt
     1021 ggttggtgcc accctcgatc cggttcgttg tcgagccgat caccaaggct tccaagttgg
     1081 ggtcgaggta ggtgaagagc actccttgac gggtcagcct ggcaagcagc tggtaggcct
     1141 tgcgcagccg gtcgtgagtc caccaccagg tggcgtgggg tcgggcccag gcgggacgaa
     1201 tatcgacctg tttgaggtag gtccgggtgt tgatcagcgg accgtaggcc tgatgccaag
     1261 cgttcaggtt gacttgccaa cggcctgctt cctcgatggt gcggatccgt gtcaatgcca
     1321 gggacaagac cctaagtgac ctgccggcgt cggtgcgtgg tcgtgtcgtc agataggtgc
     1381 ggacacctcg ttgaacgtgg acgaggcagc gttgaatcct ggtgttcggc cagcacgctt
     1441 tcaaagcgga agctagcccc gatccgccat caacaacgac gactcgcggc ggtgggatac
     1501 gttccagcag ggccgtccag gcggccgttt tttccgtgtc gcaccactgc caagcgatca
     1561 cctgcccggt ggcgccgtcg atggcgacca acaggcacca gccgtcactg agatacgtcc
     1621 cgtcgagttg gatctcgtca tagatttccc cagtcacagc gatggtcggt gccacgttcc
     1681 aacaccaggc atgttggtcc cgaaaccggc gaccggtgcg accatccacg tcgcgttgag
     1741 tggctcgacc catgagccat gccaggaacc gatccaggtc tgctgcccga gtcacatccg
     1801 ggcgtctacg ggtgattgag gaaccgcact tggggcaccg ccatctcgtg gtccccgcac
     1861 tcgttacacc gttgcgtttc agcctcgtcc cacacagcac gcacgaccct gaatgtccac
     1921 tacccgctcc agtcatgcct ccaatggcat aacacaggcc cgaaaacgcc taccgaccag
     1981 ggagaacaca ccctcagcag acacacttat ccgccgctaa cccggtttcg tctggaagag
     2041 ggagcaccat gggcgccatc aatcgcgagt ggcacgaggc gcaccgcatg ccgcctcgcg
     2101 cgaccgacga cctgcggcct caggtgagct cgaccgtcgt gccgtcccaa ccggcggtca
     2161 cgtgttgccc ctccgtcagg tgtccggaga cgatgtcctt ggccagttca ttgagcagtc
     2221 gggtctggat gagccgcttg agggggcgtg cgccgaactg tgggtcgaag ccgtccttcg
     2281 ccagggatgc gcgcaatgcg ggcgacaccg tgagcgtgat gcccttcgcc tgctcgatgc
     2341 gcgcgactgc cttcgcaagt tcagcatcga caatcccctc catggcggtc cggtcgatgc
     2401 ggtcgaagac caccacatcg tccaggcggt tgaggaattc gggacggaag gcgcgcttca
     2461 gcacctcgtg aagctgcgcc tcgaggacgc tgcggtctgc tccgtcccac gcgagcacaa
     2521 tgtcggatcc caagttcgag gtcatcacga tgatggtgtt cgtgaagttc acggtgcgtc
     2581 cgtggccgtc ggtgaggcgg ccgtcgtcca ggatctggag cagcgagttg aacacctccg
     2641 ggtgggcctt ttcgatctcg tcaaacagca ccacggcgta gggccggcgg cgcaccgctt
     2701 cggtgagctg gccgccttcc tcgtagccca catatcccgg cggggcaccg atgagccggg
     2761 aaaccgtgtg cttctccatg tattcgctca tgtcc
//
LOCUS       DASRKH010000158         2257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_53839, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000158 DASRKH010000000
VERSION     DASRKH010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53839"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1097
                     /locus_tag="VFC06_02680"
     CDS             <1..1097
                     /locus_tag="VFC06_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HZL80838.1"
                     /translation="FVARSRELGMEVALDIALQCSPDHPWVTEHPEWFTTRADGTIAY
                     AENPPKKYQDIYPLNFDNDPEGIYVAIRDMLEVWVSHGVTLFRVDNPHTKSLQFWQRL
                     LTEFRAAHPDVVFLAEAFTRPAMMRTLGAVGYHQSYTYFAWRNTKQELAEYLQEVAAE
                     TSHLVRPAFWPTTHDILTPFMQQGGTAAFALRAVLAATGSPTYGIYSGYEFIENIARP
                     GVEEQIDNEKYEFKPRDWNRANDAGIAFLLGELNRSRHQHPALQQLHQLTVHPTSNDS
                     VLAYSKRIEARFSPTGEDDVVLVVVNLDPFLTHESLVYLDMEALGLDPSSPFRVHDEL
                     NGQDHQWNATAWVRLDPQASSAHVMSVGPL"
     gene            1037..>2257
                     /locus_tag="VFC06_02685"
     CDS             1037..>2257
                     /locus_tag="VFC06_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HZL80839.1"
                     /translation="MGAPRPAGLERPRHVRRAPVTHQPIPVPTSAILAAVRTHGRAPV
                     EQDPEWFKTAVFYEVVLRAFADSDGDGTGDLRGLITHLDYLAWLGVDCLWLPPFYPSP
                     LRDGGYDVADYTAIATQYGTMAEFAELMEAAHSRGMRVIVDLVMNHTSDAHPWFQASR
                     SDPTGPYGDFYVWTDDNTEYSDARIIFVDTEVSNWTFDTVRREYYWHRFFSHQPDLNF
                     ENPDVHRALFDVVRYWCKVGVDGFRLDAIPYLYEEAGTNCENLPQTHAFLRSLRAMVD
                     EEFPGTIMLAEANQWPSDVVEYFGTDGHPECHLCFHFPVMPRIYYALREQKARAIRDI
                     LRDTPPIPAGGQWGTFLRNHDELTLEMVTPEERAAMYGWYAEDSRMRANVGIRRRLAT
                     LLGNSRAEIQLAHAL"
BASE COUNT          379 a          836 c          698 g          344 t
ORIGIN      
        1 ccttcgtcgc ccgctcccgc gagctcggca tggaggtggc gctcgacatc gcgctgcagt
       61 gctccccgga ccacccctgg gtcacggagc acccggagtg gttcaccacc cgggccgacg
      121 gcaccatcgc gtacgcggag aacccgccca agaagtacca ggacatctac ccgctcaact
      181 tcgacaacga ccccgagggc atctacgtcg cgatccggga catgctggag gtgtgggtgt
      241 cgcacggcgt gacgctgttc cgcgtcgaca acccgcacac caagtccctc cagttctggc
      301 agcggctcct cacggagttc cgggcggcgc acccggacgt cgtgttcctc gccgaggcgt
      361 tcacccgtcc cgccatgatg cgcacgctcg gcgcggtggg ctaccaccag tcgtacacgt
      421 acttcgcatg gcgcaacacg aagcaggaac tggcggagta cctgcaggag gtggcggcgg
      481 agacctccca cctggtgcgc cccgccttct ggccgaccac ccatgacatc ctcaccccat
      541 tcatgcagca gggcggcacc gcggcgttcg cgctgcgcgc cgtgctggcg gccaccggtt
      601 cgcccaccta cggcatctac tccggctacg agttcatcga gaacatcgcg cggcccggcg
      661 tcgaggagca gatcgacaac gagaagtacg agttcaagcc gcgtgactgg aaccgtgcga
      721 acgacgccgg gatcgccttc ctgctcgggg agctcaaccg gagtcgccat cagcacccgg
      781 cgctgcagca gttgcaccag ctgacggtcc acccgacatc gaacgactcc gtcctcgcgt
      841 actcgaagcg catcgaggcc aggttctcgc ccacgggcga ggacgacgtg gtcctcgtcg
      901 tcgtgaacct cgacccgttc ctgacgcacg agtcgctcgt ctacctcgac atggaggccc
      961 tgggcctcga cccgtcgagc cccttccggg tgcatgacga gctcaacggc caggaccacc
     1021 agtggaacgc gaccgcatgg gtgcgcctcg acccgcaggc ctcgagcgcc cacgtcatgt
     1081 ccgtcgggcc cctgtgacac accagcccat cccggttccc acctccgcga tcctggccgc
     1141 ggtgaggacc cacggccgcg ccccggtgga gcaggacccg gagtggttca agaccgccgt
     1201 gttctacgag gtggtgctgc gcgccttcgc cgactccgac ggcgacggga cgggagacct
     1261 gcgcgggctc atcacccacc tggactacct cgcctggctg ggtgtcgatt gcctctggct
     1321 gccaccgttc tacccgtcgc cgctgcggga cgggggctac gacgtcgccg actacaccgc
     1381 gatcgccacc cagtacggga ccatggcgga gttcgccgaa ctgatggagg cggcgcattc
     1441 ccgcgggatg cgcgtgatcg tggacctcgt aatgaaccac acctccgacg cccacccatg
     1501 gttccaggcc tcccgctcgg acccgaccgg cccgtacggc gacttctacg tgtggacgga
     1561 cgacaacacc gagtactcgg acgcccgcat catcttcgtg gacaccgagg tctccaactg
     1621 gaccttcgac acggtgcgcc gcgagtacta ctggcaccgg ttcttctccc accagccgga
     1681 cctcaacttc gagaacccgg acgtgcaccg cgccctcttc gacgtggtgc gctactggtg
     1741 caaggtgggc gtggacggct tccgactcga tgcgatcccc tacctctacg aggaggcggg
     1801 caccaactgc gagaacctgc cccagaccca cgccttcctg aggtcgctgc gtgcgatggt
     1861 ggacgaggag ttcccgggca cgatcatgct cgccgaggcc aaccagtggc cgagcgacgt
     1921 ggtggaatac ttcgggaccg acgggcaccc ggagtgccac ctgtgcttcc acttccccgt
     1981 gatgccgcgc atctactacg ccctgcgcga gcagaaggcg cgcgcaatcc gcgacatcct
     2041 ccgcgacacg ccgccgatcc ccgccggcgg gcagtggggc acgttcctgc ggaaccacga
     2101 cgagttgacg ctcgagatgg tcacgcccga ggaacgtgcc gcgatgtacg gctggtacgc
     2161 cgaggactcc cgcatgcgcg ccaacgtcgg aatccgccgc cggctcgcca cgctgctcgg
     2221 gaactcgcgc gccgagatcc agctcgccca cgcgctc
//
LOCUS       DASRKH010000159         2389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_111, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000159 DASRKH010000000
VERSION     DASRKH010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VFC06_02690"
     CDS             complement(<1..372)
                     /locus_tag="VFC06_02690"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZL80840.1"
                     /translation="MSLLPTGTPLVLLNAFPLDREQWEPLLAALDASVGDIITFDSPG
                     IGDMPASPSDPSIDLIADAAVAALREATGSQDAIWVGCSMGGYVAMAVLERHPDAVAG
                     LGLIGTRAGADTAQAHARRLEA"
     gene            complement(369..1094)
                     /locus_tag="VFC06_02695"
     CDS             complement(369..1094)
                     /locus_tag="VFC06_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZL80841.1"
                     /translation="MTVLPADRADIEIVTEDGLTLVGELALPPSGRVAVTLITLHPLP
                     THGGFMDSHVLRKASWRLPYLADVAVLRFNTRGTSSPRGTSQGQFDEGVGEGADVRAA
                     VQFAVDRGLPHRWLVGWSFGTELALMHGADLDVEGAVLLSPPLSRARREDLEHWARTE
                     KPVTVLVPEFDDFLPPDQAVTGFAPLTQARVIPVEGAKHLWVGEKYVRIVLDLVIGAV
                     VPGAVSLPTSVPVTAVVPSGERP"
     gene            complement(1608..>2389)
                     /locus_tag="VFC06_02700"
     CDS             complement(1608..>2389)
                     /locus_tag="VFC06_02700"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HZL80842.1"
                     /translation="SHVPRPWNVADPRDVAVAQKIDALANRIFMDPYAVGQYPAILRE
                     STSSITDWSFVHEGDLEAIKGTLDVLGVNYYSSHMVRFNDAPHQPSGEDGHGSTLHRC
                     WPGADDAEFMPLVGKRTTMGWNVDPSGFHSHLMRMHRDMGLPMIVTENGASWPDEVSE
                     DGRIRDVDRYTYLHDHMGALLKARDEGADIRGYMAWSLMDNFEWAYGYSKRFGLLRVD
                     YNSQVRTWKDSAYWYRETLRTRAIAPLDAIESLVETPPRVY"
BASE COUNT          420 a          771 c          802 g          396 t
ORIGIN      
        1 ggcctccagg cgccgcgcgt gggcctgtgc ggtatcggcg ccggctctcg tcccgatcag
       61 tccgagccct gcgacggcat cggggtgacg ctcgagtacg gccatggcga cgtacccgcc
      121 catcgagcag cccacccaga tcgcgtcttg cgagcccgtc gcctcgcgca gggccgccac
      181 ggcggcgtct gcgatgagat cgatcgacgg gtcgctgggt gacgccggca tgtcgccgat
      241 gccgggagag tcaaaagtga tgatgtcgcc cacggacgca tcgagggcag caagcagggg
      301 ttcccactgc tcgcgatcca gcgggaaggc attgaggagg acgagggggg ttccggtggg
      361 caacaaactc atggtctctc tcctgagggg acaactgcgg tgacgggaac ggaggtcggg
      421 agggacacgg cgcctggaac cactgcgccg atcaccagat cgaggacgat ccgcacgtac
      481 ttctccccca cccacaagtg cttggctccc tcgacgggga tcacgcgggc ttgggtcagc
      541 ggtgcgaaac cggtgacggc ttggtcaggg ggaaggaagt catcgaactc gggaacgagc
      601 acagtcacgg gcttctcagt gcgtgcccag tgctcgagat cctcccgcct ggcgcggctg
      661 agtggcggcg acaacaacac cgctccctcg acatccaggt cggcgccgtg catgagggca
      721 agctcggtgc caaaactcca gcccaccaac cagcggtgcg gtagccctct gtcgactgcg
      781 aactgcacgg ctgcgcgcac gtctgcgccc tcgcccacgc cttcgtcaaa ctggccctgc
      841 gaggttccgc gcggcgacga cgtgccgcgg gtattgaacc tgagcaccgc cacgtccgcg
      901 aggtacggca atcgccacga cgccttgcgc aacacgtgcg agtccatgaa cccgccgtgg
      961 gtgggcagag gatgcagggt gatcagggtg acggcgaccc gacccgacgg gggcaacgct
     1021 agttcgccca caagcgtgag gccgtcctcg gtgacaatct cgatgtccgc gcggtcagcg
     1081 ggcaacaccg tcatggcgcc aacctccgtc agctcagcca cgtgttctcc caaaggtctg
     1141 ccagcaggcc ctgtgccagt gtcgtcgatc ctgacccgcg gcatcggcgc caaacagtcc
     1201 gtctgccgcc cacgccacga tgtgttcctg cgtccctggc acgtctcgac cgcacgcggg
     1261 gcaggtgtag gtcttgtccg acggacgtgg aatggccacc caatagtcct cgccccgcgg
     1321 gccccgctgg cgctgggcgc caaggcctcc cctcgctcgt tccaggtcga gctccgggtg
     1381 gggctgtccg taggggcgct tggtggatcg gcgcttggac ggcatggtcc gagtctacgg
     1441 aacctttggc cttagcagct caagggaaag aagccagcgc cgcgctcgag ggggggttga
     1501 gtgcggcgcc ggacagttaa cagacgcggg gtggcgtctc taccagcgac tctaggaaag
     1561 ggagccggcg ccgcgctcga gggggggttg agcgcgacgc cggacaacta atagacgcgg
     1621 ggtggcgtct ctaccagcga ttctatcgcg tcgagtgggg caattgcacg ggttcgcagg
     1681 gtctcccggt accaataggc ggaatccttc caagtacgca cctgggagtt gtagtcaacc
     1741 ctcaaaaggc cgaagcgctt ggagtatccg taggcccact cgaagttatc catcaggctc
     1801 caggccatgt agccacggat atcggcaccc tcgtcacgcg ccttcagaag cgcgcccatg
     1861 tggtcgtgca ggtaggtgta gcggtcgacg tcgcgaatac ggccgtcttc gctcacctcg
     1921 tccggccatg aggcgccgtt ctcggtgacg atcatgggga ggcccatgtc gcgatgcatg
     1981 cgcatcaggt gtgagtggaa gccgctcgga tcgacgttcc atcccatcgt ggtgcgcttg
     2041 cccaccaggg gcatgaactc cgcgtcatcc gcaccgggcc agcaacggtg cagggtggac
     2101 ccgtggccgt cctcgcccga cggctggtga ggcgcgtcgt tgaagcggac catgtgactc
     2161 gagtagtaat tgacgcccag cacgtccagc gtgcccttga tcgcctccag gtctccctca
     2221 tgcacaaacg accagtcggt gatgctcgac gtagattcac gcaggatcgc cggatactgg
     2281 cccactgcat agggatccat gaagatcctg ttggcaaggg catcgatctt ctgcgcgacg
     2341 gcgacgtctc gggggtcggc cacattccac ggccgaggga cgtgagagt
//
LOCUS       DASRKH010000160         2195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_59920, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000160 DASRKH010000000
VERSION     DASRKH010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59920"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..1179)
                     /locus_tag="VFC06_02705"
     CDS             complement(58..1179)
                     /locus_tag="VFC06_02705"
                     /inference="COORDINATES: protein motif:HMM:NF021567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2293 domain-containing protein"
                     /protein_id="HZL80843.1"
                     /translation="MSSSRPTVSAPRHRRPSLFVMDQEYLTKLERRVTSAMEAALQHD
                     KFVSPLEVLVGIGWLSRSSIEAWRHARIACLEQEAAVTTEKLALVLEVLDRHATRQGL
                     IPVESLYVAASRDRSHLHFTSGGNESLERAYRTHWFSPEVSVTKRDRITHRTSRAPDL
                     VVISALQAWTCTICGGTGSLLFMEGDGPLCLSCADMDHLVFLPAGDAALSRRAKKASR
                     LRAVVVRFSRSRKRYERQGVLVEEAALADAERLCLADEDARARRAARDRERRSEEDGE
                     LRDTWAREISRLFPSCPAWRAARISSHAVERGSGRVGRSAAGRAVDERAVRSAVVASI
                     RHEDTDYDELLMSGTERAVAREQVRRSVDEVLERWRSVP"
     gene            complement(1282..1947)
                     /locus_tag="VFC06_02710"
     CDS             complement(1282..1947)
                     /locus_tag="VFC06_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80844.1"
                     /translation="MLEQFLAEQREKLAPRTIARYEEVIELLRHCLNGYAYQSLYGLE
                     KKRWEKAFEDGDEEAFSKLFGPEKIIENYGEFLGYFMIRKVMASQELLKASGTVTKKL
                     AKWLAERGLVTDDDSAVAVERATEASVDLPKAEKLSSLLYEQTRSVPRFNPDDIADED
                     WVEDYLMIDRVEPDALWLEGEVGPVIVPGVGRIATAGWSVNIVMARLEGVWRVVEVGN
                     VYP"
BASE COUNT          412 a          723 c          652 g          408 t
ORIGIN      
        1 gagcggagtt gcccacttgc gctatgcgtt gttggagctt gcgctcgttg tcagggttca
       61 cgggacggac cgccagcgct cgagaacctc atcgacgctt cgccgtacct gctcgcgggc
      121 gactgctcgc tcagtacctg acatgagcaa ctcgtcgtag tcggtgtctt cgtgacgaat
      181 cgaagcgaca acggccgacc ggacagcccg ctcatcgacg gcacggcccg cggctgagcg
      241 ccccacgcga ccacttcccc gctctacagc atggctggag atcctagctg cccgccacgc
      301 cgggcagctg ggaaagagcc ggctgatctc tcgcgcccag gtatctcgca gctccccatc
      361 ctcttcggat cgccgctcac gatcccgggc agctcgccgt gctcgggcgt cttcatcagc
      421 cagacaaagt ctctcggcgt cggccagcgc agcttcctcg acgaggacac cttggcgctc
      481 gtatcgcttg cgcgaacggc tgaagcgcac aacaaccgcg cggagacgac tcgctttctt
      541 cgctcgcctg ctcaaagccg cgtctcccgc cggaagaaag acgagatggt ccatgtcggc
      601 gcagctcagg cagagtgggc catctccttc catgaacaac agcgaacctg ttcccccgca
      661 gatggtgcac gtccatgcct gcagtgcaga gatgaccacc agatcaggcg cgcgactcgt
      721 gcgatgcgtg atccggtcgc gtttggtcac tgacacctca ggcgagaacc agtgcgtgcg
      781 atacgcgcgt tcgagagact cgttgccacc gctcgtaaaa tgaaggtgtg aacggtctcg
      841 cgacgcggct acatacaaag actccacggg tatgaggcct tgccgggtgg cgtggcgatc
      901 caacacttcc aataccaagg cgagcttttc tgtcgtcacc gcggcctctt gctccaagca
      961 ggcgatacgt gcatgtctcc aagcctcgat cgaggatcga gacagccagc cgataccgac
     1021 cagaacctcc agtggtgaaa cgaacttgtc gtgctgcagc gccgcctcca tggcagacgt
     1081 cacgcggcgc tcgagcttgg tcaggtactc ctggtccatt acgaacagcg aagggcgtcg
     1141 atggcgcggg gcagagacgg tggggcggga tgagctcatg taagcacctg gcgaactcgt
     1201 cgcagcttga cggagacctt cacagcacgg cgaaagggga aacccactgt atacagccga
     1261 ctggaacgga gatcccgaca ctcaggggta cacgttgccg acctcgacca cgcgccaaac
     1321 gccttctagt cgagccatca caatgttcac cgaccatccc gccgttgcga tcctgccgac
     1381 gccgggaact atgacaggtc cgacctctcc ttcgagccac agcgcatcgg gctcaactcg
     1441 gtcgatcatg agatagtcct caacccagtc ctcatcggcg atgtcatccg gattgaaccg
     1501 tggcaccgag cgggtctgct cgtaaagcaa gctgctcaac ttctcggcct tcggcaaatc
     1561 gaccgacgct tcggtcgcac gttcgacggc aactgcgctg tcgtcatcgg ttaccagacc
     1621 tcgctcggcg agccacttgg ccagcttctt ggtcacggtg cctgatgcct tgagcagttc
     1681 ctggctggcc atcaccttac ggatcatgaa gtagccgagg aactccccgt agttctcgat
     1741 gatcttctct gggccgaaga gcttggagaa cgcctcctcg tcgccatcct cgaaggcttt
     1801 ctcccaacgc ttcttctcaa gcccataaag tgactggtag gcgtagccgt tgagacagtg
     1861 tcgaagcagt tcgatgacct cctcgtacct cgcgatcgtg cgaggagcga gcttctcccg
     1921 ctgctcagcg aggaactgtt cgagcacgat gtcaatcgag ggtgcatgac ctgccatgcc
     1981 gtccatcctg gcagaccacg gccgggacca agtgccgggc tgatggaagg atcgcctttg
     2041 gggacctcag atttcccatg ggggctggcc tgtcaagggg gtcggcggcg cggcgctggt
     2101 acggcccgcc cccacgcgtc aagggcgctg cgcgtccctg cgggaccggc tgcgccggcc
     2161 cttgacccgc gggtccctgc cggtcctggg caggg
//
LOCUS       DASRKH010000161         2425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_45393, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000161 DASRKH010000000
VERSION     DASRKH010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45393"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="VFC06_02715"
     CDS             <1..717
                     /locus_tag="VFC06_02715"
                     /inference="COORDINATES: protein motif:HMM:NF025199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4191 family protein"
                     /protein_id="HZL80845.1"
                     /translation="APPAPKQRWYKTLWQAYSVTAKTDKALPWILGSILLAPLIVGIV
                     VGVLSDGLAAKVYGPLTGIMIGAVAALFVLTKRFEKQMFQQMDGVMGGSLAVAQSITK
                     GWQFAEEPIAVDQKTRAVIFQGVGNGGVVLLAEGGNSARRQVTQTVARLGKLVPGVPV
                     TPIYVGHGEGEVELKRLTKVIRATTTKHFGRGLRKGLSSADAETVRARLRAMGGPQMA
                     VPKGIDPTKARADRKAMRGR"
     gene            931..2364
                     /gene="glnA"
                     /locus_tag="VFC06_02720"
     CDS             931..2364
                     /gene="glnA"
                     /locus_tag="VFC06_02720"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HZL80846.1"
                     /translation="MFKNAEEALAYVKEEGVEYVDIRFCDLPGVMQHFNIPAHQFTED
                     VFADGAMFDGSSIRGFQTINESDMKLLPDVATAYIDPFRVAKTLIINFSIVDPFTNEP
                     YSRDPRNVAAKAEAYLKTTGIGDTAFFAPEAEFFIFDSVRFATNQHEGYYHIDSSEAA
                     WNTGIEYEEDGTPNRGYKTRYKGGYFPVSPADQFADLRDEMCTVLGQVGLDLERAHHE
                     VGTAGQQEINYRFNTLLHAADDLMKFKYVVKNVAWAAGKTVTFMPKPLFGDNGSGMHV
                     HQSIWEDGTPLFFDEKGYGGLSDIARWYIGGILKHAPSLLAFTNPTVNSYHRLVPGYE
                     APVNLVYSARNRSAAVRIPITGSNPKAKRIEFRAPDSSGNPYLAFAALLMAGLDGIKN
                     RIEPPAPVDKDLYELPPEEHARIPQVPSSLEGVLNSLEADHAFLLEGGVFTPDLIETW
                     IDLKRTQEIDPLRFRPHPHEFELYYDC"
BASE COUNT          454 a          846 c          711 g          414 t
ORIGIN      
        1 gcaccccccg cacccaagca gcgttggtac aagactctct ggcaggccta ctcggtcacc
       61 gcgaagaccg acaaagcact cccgtggatc ctcggcagca tcctcctcgc gcccctcatc
      121 gtgggcatcg tcgtgggtgt cctttccgac ggactggccg ccaaggtcta tggccccctc
      181 accggcatca tgatcggcgc cgtagcggcg ttgttcgtgc tgaccaagcg cttcgagaag
      241 cagatgttcc agcagatgga cggagtcatg ggcggctcgc ttgcggtcgc tcagtcgatc
      301 accaagggct ggcagtttgc cgaggagccc atcgctgtcg accagaagac ccgcgcggtg
      361 atcttccagg gcgtcggcaa cggcggagtg gttctgctgg cagaaggcgg caactcagca
      421 cggcgacagg tcacgcagac cgtggcccgg ctaggcaaac tcgttcccgg cgtgccggtc
      481 accccgatct acgtggggca tggcgaaggc gaagtggagc tcaagcgtct caccaaggtg
      541 attcgcgcca ccacgaccaa gcatttcggt cggggtttgc gcaaggggct ttcttcggcc
      601 gatgccgaga ccgtgcgggc gcgcctgcgc gccatgggcg gcccgcaaat ggccgtgccg
      661 aagggcatcg accccaccaa ggcccgcgcc gaccgcaagg cgatgcgcgg ccgctaggcc
      721 cacccccaga gcgccattgt cgtcgaatct ccatcactgg ggcgccgcag cgtcgactca
      781 caggaccttc ggcccggttt ctggcccgtc cccgcacggc cgatggcgcg tcgtaacgtg
      841 cgcgtaacac acggaatacg cacgggtaat gcactgccac taagttgggt gcagccacag
      901 ttggcttgac ctacctcaag gagcaatccg atgttcaaga acgccgaaga ggcgctcgcc
      961 tatgtgaagg aagagggcgt cgagtacgtc gacatccgct tctgcgacct gcctggcgtg
     1021 atgcagcact tcaacattcc ggcccaccag ttcaccgagg acgttttcgc ggatggcgcc
     1081 atgttcgacg gatcgtcgat ccgtggattc cagaccatca acgagtccga catgaagctg
     1141 ctgcccgacg tggccacggc ctacattgac ccgttccgcg tcgcgaagac gctgatcatc
     1201 aacttctcga tcgtggaccc gttcacgaac gagccctact cgcgcgaccc ccgcaacgtc
     1261 gccgcgaagg ccgaggccta cctcaagacc accggcatcg gtgacaccgc gttcttcgca
     1321 cccgaggccg aattcttcat cttcgactcc gtgcgattcg cgaccaacca gcacgagggt
     1381 tactaccaca tcgactcctc cgaggccgcg tggaacaccg gcatcgaata cgaggaggac
     1441 ggcaccccca accgcggtta caagacccgc tacaagggtg gctacttccc cgtcagcccc
     1501 gccgaccagt tcgctgacct gcgcgacgag atgtgcaccg tcctcggcca ggtgggtctt
     1561 gacctggagc gcgcacacca cgaggtgggc accgcaggtc agcaggagat caactaccgc
     1621 ttcaacacgc tgctgcacgc cgctgacgac ctgatgaagt tcaagtacgt cgtcaagaac
     1681 gtggcctggg ccgccggcaa gacggtgacc tttatgccca agccgctctt cggagacaac
     1741 ggttccggca tgcacgtgca ccagtcgatc tgggaagacg gcaccccgtt gttcttcgat
     1801 gagaagggct acggcggcct gtccgacatc gcgcgctggt acatcggcgg catcctgaag
     1861 cacgcgccct cgctgctcgc gttcactaac ccgacggtca actcgtacca ccgcctcgtg
     1921 cctggctacg aggcccccgt caacctggtg tactcggctc ggaaccgctc cgccgcggtg
     1981 cgtatcccga tcacgggatc gaaccccaag gccaagcgca tcgagttccg cgcgcctgac
     2041 tcgtcgggca acccgtacct cgcgttcgcc gcgctcctca tggccggcct cgacggcatc
     2101 aagaaccgca tcgagccccc ggcccctgtg gacaaggacc tgtacgagct tcctcccgag
     2161 gagcacgccc ggattcctca ggttccctcc tcgcttgagg gcgtgctcaa tagcctcgag
     2221 gccgaccacg cattcctcct cgagggcggc gtgttcacgc ccgacctcat cgagacgtgg
     2281 atcgacctca agcgcacgca ggagatcgac ccgctgcgtt tccgtccgca cccccacgaa
     2341 ttcgagctgt actacgactg ctaggtacta gtagccgctt tacctggggc ttcgtcattt
     2401 taacaccgcc ccagtccgca gagag
//
LOCUS       DASRKH010000162         2314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_18005, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000162 DASRKH010000000
VERSION     DASRKH010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18005"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="VFC06_02725"
     CDS             complement(<1..785)
                     /locus_tag="VFC06_02725"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZL80847.1"
                     /translation="MARSSWHDNTRHGDTRHDDQPARPDDDTVGAATRAIADATAALT
                     KLLGNQLASVSTELGDAIATSLRDAARGLADASETVERRSGGQRAEERRRARVDRTRA
                     DLLAAAGRVFAAQGYEGASVGDIAAEAGYTKGALYAHFGSKSDLFVALARERLDCDRP
                     TAPTAGSATGDLADELSAGVAAADDDPAMLLALEMLAYAVRHPEARAELGALFDASLQ
                     GLAERVRDDRLARDRSAGREPDATPGVTRDDLDTALGLLAVSNV"
     gene            879..1856
                     /locus_tag="VFC06_02730"
     CDS             879..1856
                     /locus_tag="VFC06_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZL80848.1"
                     /translation="MEPSTVLSIRGLSKRYPGRRGVQANDGIDLDVLAGQVVGLLGHN
                     GAGKTTLVNQVVGLVRPDAGTIWLDGIDAVADPDGARRLASIQAQANVPITGLTPRTA
                     IQLVGRIRGGSRRDVAARTQGLIEALDIGSWADMPSQKISGGIARLTAFCMTAVVPGR
                     IVVLDEPTNDVDPVRRRQLWDAIRTLADDGAGVLLVTHNVREAERVVDQLVILDQGRV
                     IAADTPAGLAASTRGVLTVELDLVPSTGGDIGWPPGVTETTRERLRAAGTVPAELAAD
                     VVRWAQAQVDAGSVERYALAAASLEDVYVRLVGSDRAPVATDAELEVVA"
     gene            1853..>2314
                     /locus_tag="VFC06_02735"
     CDS             1853..>2314
                     /locus_tag="VFC06_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZL80849.1"
                     /translation="MTAVRQGLLLLQWQLRRGSTVMPLLVVVQIAMAVATVVGYGLLV
                     GDPTPDVALYLATGAPTITLITVGLVMTPQMLGQAKTEGSLDWMRTLPVPRWTFLASD
                     LLMWTLIALPGMVLGIVAGVMRFDVQLSIAPWLVAAALLVSLTSASVGYAMA"
BASE COUNT          280 a          839 c          854 g          341 t
ORIGIN      
        1 acgttgctga ccgcgaggag cccgagggcg gtgtcgaggt cgtcgcgcgt cacgcccggg
       61 gtcgcgtcgg gctcgcgacc tgcgctgcgg tcccgggcga gccggtcgtc gcgcacgcgc
      121 tcggccagtc cctgcaggga cgcgtcgaac agcgcgccga gctcggcgcg cgcctcgggg
      181 tgacggacgg cgtacgccag catctcgagc gcgagcagca tcgccgggtc gtcgtccgcg
      241 gcggccacgc ctgcggacag ctcgtcggcg aggtcgccgg tcgctgagcc ggcggtgggg
      301 gcggtcggtc ggtcgcagtc caggcgttcg cgagcgaggg cgacgaagag gtcgctcttg
      361 gacccgaagt gcgcgtagag cgcgcccttg gtgtagccgg cctcggccgc gatgtcgccg
      421 actgaggcgc cctcgtaccc ctgcgcagcg aagactcgac cggccgcggc cagcaggtcg
      481 gcgcgggtgc ggtcgacgcg ggcccgccga cgctcctcgg cgcgctggcc tcccgaccgg
      541 cgctcgaccg tctcggacgc gtcggcgaga cctcgcgccg cgtcgcgcag gctcgtggcg
      601 atcgcgtcgc ccagctcggt gctgacactc gcgagctggt tgccgagcag cttggtgaga
      661 gccgcggtcg cgtcggcgat ggcgcgggtc gccgcgccga ccgtgtcgtc gtccgggcgc
      721 gcgggctggt cgtcgtgccg ggtgtcgccg tgccgggtgt tgtcgtgcca gctggagcgt
      781 gccatggggc gatcatacgc cgaagacccc ttctatatac cgaccggtat ctgctacgtt
      841 ctggcatata ccaatcagta catcgacagg agacggtcat ggagccgagc accgtgctca
      901 gcatccgcgg gttgagcaag cggtacccgg ggcgccgtgg cgtccaggcg aacgacggga
      961 tcgacctcga cgtcctggcg ggtcaggtcg tcggtctgct cggtcacaac ggtgcgggca
     1021 agacgactct cgtcaaccag gtcgtcggcc tcgtacgacc cgacgcgggc acgatctggc
     1081 tcgacggcat cgacgcggtc gccgacccgg acggggcgcg gcggctcgcc tcgatccagg
     1141 cgcaggcgaa cgtcccgatc accgggctca cgccgcgcac cgcgatccag ctcgtcggac
     1201 gcatccgcgg cggcagccgg cgcgacgtcg ccgcgcgcac ccaggggctc atcgaggccc
     1261 tcgacatcgg cagctgggcc gacatgccgt cgcagaagat ctccggcggc atcgcgcgcc
     1321 tcaccgcgtt ctgcatgacc gcggtcgtgc ccggccgcat cgtcgtgctc gacgagccga
     1381 cgaacgacgt cgaccccgtg cgccggcggc agctctggga cgcgatccgc accctggccg
     1441 acgacggcgc gggcgtgctc ctcgtgaccc acaacgtgcg cgaggccgag cgcgtcgtcg
     1501 accagctcgt gatcctcgac cagggcaggg tgatcgcggc cgacaccccc gcggggctcg
     1561 cggcgtcgac gcgcggcgtc ctcacggtcg agctcgacct cgtcccgtcc acaggtggcg
     1621 acatcggctg gccgccgggt gtgaccgaga cgacccgcga gcgtctgcgc gccgccggga
     1681 cggtgcccgc cgagctggcc gccgacgtcg tgcgctgggc gcaggctcag gtcgacgcgg
     1741 gttccgtcga gcggtacgcg ctcgcggcgg cgtcgctcga ggacgtctac gtgcgcctcg
     1801 tcgggtccga ccgtgccccc gtggccaccg atgccgagct ggaggtcgtg gcatgaccgc
     1861 cgtgcgtcag ggcctgctcc tgctgcagtg gcagctgcgt cgcgggtcga ccgtcatgcc
     1921 cctcctcgtc gtcgtgcaga tcgccatggc cgtggccacc gtcgtcggct acgggctcct
     1981 cgtgggcgac ccgacgcccg acgtcgcgct ctacctcgcg acgggtgcgc cgacgatcac
     2041 gctcatcacc gtgggcctcg tcatgacgcc gcagatgctc ggccaggcca agaccgaggg
     2101 cagcctcgac tggatgcgca cgctccccgt gccgcgctgg accttcctcg cgagcgacct
     2161 gctcatgtgg acgctcatcg cgctccccgg gatggtgctc ggcatcgtcg cgggtgtcat
     2221 gcggttcgac gtgcagctct cgatcgcgcc gtggctcgtc gcggccgcgc tgctcgtctc
     2281 gctgacgtcg gcgtcggtcg ggtacgcgat ggcg
//
LOCUS       DASRKH010000163         2132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_45854, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000163 DASRKH010000000
VERSION     DASRKH010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45854"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VFC06_02740"
     CDS             <1..439
                     /locus_tag="VFC06_02740"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HZL80850.1"
                     /translation="KANPDAKLCLETEFPDRPDGLILFSDATGYTVPQDQIQILDTGL
                     IYTETANGACQFGEVFTTDGRITALNLSLVADPGVFILYNISYTWNDAVYQENADVYK
                     RLADEIFAPLDAEKMASLNAAVDVEGRKAEDVAAEYLKEIGLL"
     gene            complement(536..1399)
                     /locus_tag="VFC06_02745"
     CDS             complement(536..1399)
                     /locus_tag="VFC06_02745"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZL80851.1"
                     /translation="MSDRKETDGPNTLGRAAIVAGGMAGVPAVGVRESSAASTRPSRN
                     RPIGLILAPLGILALAAALLLYYRGLGLDSEDNFQQASALDWSVKLWPQTVEQVTIAF
                     WSTVLVVLIAVPAGIALTRPAMRRYSPAVLSIANTGQALPAYGLLVIFLVMLGQGLTT
                     VIWALVLYALLPVLRNTMVGLDGVDKATIEAGRGMGMTKFRALVRIELPLAVPIIIAG
                     VRTAMIINIGMATLAFLIGGGGLGITISSGLKLQQDPVLLVGAGLVAIVALSFDWLGA
                     VAERYLKPRGL"
     gene            complement(1396..>2132)
                     /locus_tag="VFC06_02750"
     CDS             complement(1396..>2132)
                     /locus_tag="VFC06_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HZL80852.1"
                     /translation="GQAQRVGVARALGADPDVMLMDEPFGAIDPITRDRLQNEFLRLQ
                     AVLGKTIVFVTHDIDEAMKMGDRIAILGERSAVRQIDTPERILALPADDFVDDFIGTG
                     STLKGLHFERVSGLDLKPYPTMTLDEPFEAGLATLRADGREWLLLLDGERRPVRWLRA
                     ADLKRGGAGRDLLTRGQHVRSRVEENATLHDTLEEMLKSSTGQACVVDGKGRFVGAVE
                     INHLTDVIRRLRQEAQAHYDSLEGSS"
BASE COUNT          363 a          720 c          717 g          332 t
ORIGIN      
        1 caaggcgaac cccgacgcca agctctgctt ggagacggag ttcccggatc gacccgacgg
       61 gctgatcctc ttcagtgacg cgaccgggta cacggttccg caggaccaga tccagatcct
      121 ggacaccggc ctgatctaca cagagaccgc caacggcgcg tgccagttcg gcgaggtttt
      181 cacgaccgac ggtcgcatca cggcgctgaa cctcagcctc gtggctgacc cgggggtctt
      241 catcctctac aacatctcct acacctggaa cgacgccgtc taccaggaga acgcagacgt
      301 ctacaagcgc ctcgcggatg agatcttcgc gccgctcgac gcggagaaga tggcatcgct
      361 caacgccgcg gtcgacgtcg aaggcagaaa ggccgaggac gtcgcggccg agtacctcaa
      421 ggagatcggc ctcctgtagg cccacgcacc acgacgaaac tgggccccca cctgtcgaag
      481 gtgggggccc agtttcgtgt gagaggtccg gtggtgcgag ggccgtgcgg cgcccctaga
      541 gcccgcgagg tttcaggtac cgctcggcaa ctgcgccgag ccagtcgaag ctgagcgcga
      601 cgatcgccac gaggccggct ccgacgagga gcactgggtc ctgctggagc ttcaggccgg
      661 agctgatggt gatcccaagc ccaccgcctc cgatcaggaa ggccagcgtc gccatcccga
      721 tgttgatgat catggcagtt cgcacgccgg cgatgatgat cgggacggcc agggggagct
      781 cgatccgcac gagcgcccga aacttggtca tgcccatccc gcgaccggcc tcgatcgtcg
      841 ccttgtcgac cccgtccagc cccaccatgg tgttgcgcag cacaggcagg agcgcgtaca
      901 ggacgagcgc ccagatgacc gtggtgagcc cctggccgag catcacgagg aagatgacga
      961 gcagcccgta ggcgggcaac gcctgcccgg tgttggcgat gctcagcacg gccgggctgt
     1021 agcggcgcat cgccggtcgg gtgagcgcga tacctgcggg cacggcgatc agcacgacga
     1081 ggactgtcga ccagaacgcg atggtgacct gttcaaccgt ctgcggccag agcttgacgc
     1141 tccagtcgag cgcgctggcc tgctggaagt tgtcctccga gtcgaggccg aggccgcggt
     1201 agtagagcaa cagcgccgcc gccagggcga ggatgccgag cggcgccagg atcaggccga
     1261 tgggccggtt ccgggacgga cgggtgctgg cggcggaact ttcgcggacg ccgaccgcgg
     1321 gaacgcctgc catgccgccg gcgacgatgg ccgcacgtcc cagggtgttc ggcccgtcgg
     1381 tctctttgcg gtcgctcacg aggacccctc caggctgtca tagtgcgcct gtgcttcctg
     1441 tcggagcctg cggatgacgt cagtgaggtg attgatctcc acggcgccca cgaaccgacc
     1501 cttgccgtcg accacacagg cctgcccggt ggaggacttc agcatctcct ccagggtgtc
     1561 gtgaagggtc gcgttctcct cgacccggct gcgcacgtgc tgccctcgag tgagcaggtc
     1621 gcggcccgca ccgccccgct ttaggtccgc cgctcgcagc caccgcactg gacgtcgctc
     1681 accgtccagc agcaggagcc actcgcgccc gtcggctcgt agcgtcgcca ggccggcctc
     1741 gaacggctcg tccagcgtca tcgtcgggta cggcttgagg tcgaggccgc tcacccgttc
     1801 gaagtgcaga cccttgagcg tggagccggt gccgatgaag tcgtcgacga agtcgtcagc
     1861 aggcagggcc agaatccgct cgggggtgtc gatctggcgg acggcgctgc gctcgccgag
     1921 gatggcgatc cggtcgccca tcttcatggc ctcgtcgatg tcgtgggtaa cgaagacgat
     1981 cgtcttgccg agcacggcct gaagccgcaa gaactcgttc tgtagccggt ccctggtgat
     2041 cgggtcgatg gcaccgaagg gctcgtccat cagcatcacg tccgggtccg caccgagggc
     2101 gcgtgccacc ccgacccgct gggcctgccc gc
//
LOCUS       DASRKH010000164         2360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_54053, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000164 DASRKH010000000
VERSION     DASRKH010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54053"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1252)
                     /locus_tag="VFC06_02755"
     CDS             complement(<1..1252)
                     /locus_tag="VFC06_02755"
                     /inference="COORDINATES: protein motif:HMM:NF017930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N'-diacetylchitobiose phosphorylase"
                     /protein_id="HZL80853.1"
                     /translation="MRYGHFDDAAREYVITRPDTPRSWSNYLGSRLYGGIVTQNGGGY
                     SFFRSGGTGRLLRMRFNGVPQDEPGRFVYLRDDATGEYWSATWQPVGKPLDGPDAYRA
                     QVRHGLGYSIFEASYAGIDSELTMFVPQDQAFEHWALTITNPGPEPRALSVFSYAELA
                     NEWNYRQDLENLQYSQYVVLASYRDGFIHRRNSTRDAFSECWFALSGAEVASFDTDRD
                     VFLGPYRTQSAPLAVERGECSGSETVGDNACASLHARLELAPGESRQVVFTLGVGSPD
                     APWSDGTETVRPGREVVAEYARPERIAAELEAIRTGWADRLAPFQVRTPDPQLDSMVN
                     VWHAYQTHMTFNWSRGVSLIEAGDRDGLGYRDTAQDILSVVHAIPEAARERLDLLITG
                     QTAEGGALPLIKPLTHTPGKEPTPR"
     gene            complement(1291..>2360)
                     /locus_tag="VFC06_02760"
     CDS             complement(1291..>2360)
                     /locus_tag="VFC06_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013115401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HZL80854.1"
                     /translation="FNFTVDNLGPHGLPLIGRADWNDCLNLNCFSTEPGESFQTTENQ
                     AGGVAESVFIAAQFVYAGPEYAELAERRGLTEVAAKARQAVEDMRATVLRHGWDGEWF
                     LRAYDFYGNKIGTDEKPEGKIWIEPQGFAVMAGIGTDEPADGSRSVAVRALDSVNEML
                     GTDHGLVLQYPAYTSYQIELGEVSTYPPGYKENGGIFCHNNPWVIIGETVVGNGARAF
                     DYYKRITPAYREDISDVHKLEPYVYAQMIAGKQAVRHGEAKNSWLTGTAAWNFVAVSQ
                     YLLGVRPGYDGLIVDPQIGPEVPQFTVTRSARGATYEITVTNSGTPGARGSLVVDGIP
                     IEGNTVPYAPAGSTVTVEVTV"
BASE COUNT          329 a          792 c          869 g          370 t
ORIGIN      
        1 gccggggcgt cggctccttg ccgggcgtgt gcgtaagggg cttgatgagc ggcagtgcgc
       61 cgccctcggc ggtctggccc gtgatgagca ggtcgaggcg ctcgcgggcc gcctcgggga
      121 tcgcgtggac caccgagagg atgtcctggg ccgtgtcgcg gtacccgaga ccgtcgcggt
      181 cgcccgcctc gatgagggac acgccccgcg accagttgaa cgtcatgtgc gtctggtagg
      241 cgtgccagac gttgaccatc gagtcgagct gcgggtcggg tgtgcggacc tggaacggcg
      301 cgagccggtc ggcccacccg gtgcggatcg cctcgagctc ggcggcgatg cgctcgggac
      361 gggcgtactc ggccacgacc tcgcgcccgg gacgcacggt ctcggtgccg tcgctccacg
      421 gcgcgtcggg cgagccgacc ccgagcgtga agacgacctg gcgggactcg cccggcgcga
      481 gctcgaggcg cgcatgcagc gacgcgcacg cgttgtcgcc gacggtctcg ctgcccgagc
      541 actcgccgcg ctcgaccgcg aggggcgcgg actgcgtccg gtacgggccg aggaacacgt
      601 cacggtcggt gtcgaacgac gccacctcgg cgcccgagag cgcgaaccag cactcgctga
      661 aggcgtcgcg ggtcgagttg cgacggtgga tgaacccgtc gcggtagctc gccagcacga
      721 cgtactggct gtactggagg ttctcgaggt cctggcggta gttccactcg ttggccagct
      781 cggcatagga gaagaccgac agcgcgcgcg gctcggggcc gggattggtg atcgtgaggg
      841 cccagtgctc gaaggcctgg tcctgtggga cgaacatcgt cagctcggag tcgatgcccg
      901 cgtaggacgc ctcgaagatc gagtagccca gaccgtgccg gacctgcgcc cggtacgcgt
      961 cgggcccgtc gagcggcttg ccgaccggct gccaggtcgc cgaccagtac tcgccggtcg
     1021 cgtcgtcgcg caggtagacg aagcggcccg gctcgtcctg cgggacgccg ttgaagcgca
     1081 tccgcaggag cctgccggtg ccacccgacc ggaagaagct gtacccgccg ccattctgcg
     1141 tgacgatccc gccgtacagc cgcgacccga ggtagttgct ccacgaccgg ggggtgtccg
     1201 ggcgcgtgat cacgtactcg cgagcagcgt cgtcgaagtg tccgtaccgc atcctggcgt
     1261 gtcctcctgc cgtgccgtcc gacccgaggg tcagacggtg acctcgaccg tgaccgtcga
     1321 gccggcgggc gcgtacggga ccgtgttgcc ctcgatcggg atgccgtcga cgacgagcga
     1381 gccgcgggcc ccgggcgtgc cggagttcgt gacagtgatc tcgtacgtgg cgccccgcgc
     1441 ggagcgggtc acggtgaact gcgggacctc gggaccgatc tgcgggtcga cgatgagccc
     1501 gtcgtacccg gggcggaccc cgagcaggta ctggctgacc gcgacgaagt tccaggcggc
     1561 cgtcccggtg agccacgagt tcttggcctc accgtgccgc acggcctgct tgcccgcgat
     1621 catctgggcg tacacgtacg gctcgagctt gtggacgtcg gagatgtcct cgcggtaggc
     1681 gggcgtgatc cgcttgtagt agtcgaacgc gcgcgcaccg ttgccgacga ccgtctcgcc
     1741 gatgatgacc caggggttgt tgtggcagaa gatgccaccg ttctccttgt acccgggcgg
     1801 gtacgtggag acctcgccga gctcgatctg gtagctcgtg tacgcggggt actggagcac
     1861 gaggccgtgg tcggtgccga gcatctcgtt gaccgagtcg agcgcgcgga ccgcgacgga
     1921 ccggctgccg tcggccggct cgtcggtgcc gatcccggcc atgaccgcga agccctgcgg
     1981 ctcgatccag atcttgccct cgggcttctc gtcggtcccg atcttgttgc cgtagaagtc
     2041 gtaggcgcgc aggaaccact cgccgtccca gccgtggcgc agcaccgtgg cccgcatgtc
     2101 ctcgacggcc tgacgggcct tcgcggcgac ctcggtcagc ccgcgacgct cggccagctc
     2161 ggcgtactcc ggcccggcgt acacgaactg cgcggcgatg aagaccgact cggcgacccc
     2221 gcccgcctgg ttctcggtcg tctggaagga ctcgcccggc tcggtcgaga agcagttgag
     2281 gttgaggcag tcgttccagt cggcgcggcc gatgagcggc aggccgtgcg gcccgaggtt
     2341 gtcgaccgtg aagttgaacg
//
LOCUS       DASRKH010000165         4208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_8620, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000165 DASRKH010000000
VERSION     DASRKH010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8620"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /gene="yidC"
                     /locus_tag="VFC06_02765"
     CDS             complement(<1..863)
                     /gene="yidC"
                     /locus_tag="VFC06_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HZL80855.1"
                     /translation="MEWFDGLLYPIMVAVAWIMVQFHTLLTAIGLDPASGAAWGLSIV
                     GLVIVIRIVLIPLFVKQIKASRGMQMLSPEMQAIQKRYKGKSDPASREAMSRETMELY
                     KKHGTNPFASCLPILAQSPIFFALFRVLYSLPKLSTGTYAKSTIGPLDATIAGQVEHA
                     TIFGAPLSGYFMAKDGGATVKIVTVVLIIAMSLTTFLTQRQLTMKNMPPAALEGPMAQ
                     QQKMLLYFMPLIFAFSGINFPIGVLIYWTTTNLWSMGQQFYTIRRMPAPGSAAELALK
                     ARQAKKAAAKG"
     gene            complement(888..1196)
                     /gene="yidD"
                     /locus_tag="VFC06_02770"
     CDS             complement(888..1196)
                     /gene="yidD"
                     /locus_tag="VFC06_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HZL80856.1"
                     /translation="MTKVSMGSVARAVVRFPGRVLLVLIRGYQLYISPMRPPTCRFYP
                     SCSQYAVIAVTRHGAIRGTGLAAWRLLRCNPWNIGGVDDVPPAHDRQCHHHHVASATS
                     "
     gene            complement(1208..1570)
                     /gene="rnpA"
                     /locus_tag="VFC06_02775"
     CDS             complement(1208..1570)
                     /gene="rnpA"
                     /locus_tag="VFC06_02775"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HZL80857.1"
                     /translation="MLPAAHRMRRPAEFEQAVRRGARGGRESLVVHLATRTDPGPGPV
                     VGFVVSKSIGNAVTRNKVKRRLRALMRERLDSMPSDAGVVVRALAPAATRGYADLGAD
                     LDTALETAARRARRAVRG"
     gene            complement(1582..1719)
                     /gene="rpmH"
                     /locus_tag="VFC06_02780"
     CDS             complement(1582..1719)
                     /gene="rpmH"
                     /locus_tag="VFC06_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013428281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HZL80858.1"
                     /translation="MTKRTFQPNNRRRAKTHGFRLRMRTRAGRAILAARRRKGREELS
                     A"
     gene            2247..3722
                     /gene="dnaA"
                     /locus_tag="VFC06_02785"
     CDS             2247..3722
                     /gene="dnaA"
                     /locus_tag="VFC06_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HZL80859.1"
                     /translation="MSSQDDQPARVWAQAVAELEHSPDITPRQIAFVKLAKPLGLLDG
                     TMLLAVGNDLTKEYLETRVRTEVTEALTSALGREARFAITIDVDLASDEPAISATRPA
                     ATNEVARPHDAPSHRDTVHDDDDGHSHDSQPFRPGSGRRPSTPSEPARLNKNYQFETF
                     VIGSSNRFAHAAAVAVAEAPAKAYNPLFIYGDSGLGKTHLLHAIGHYAQNLYPSVRVR
                     YVNSEEFTNDFINSIRDDKAGAFQRRYREVDVLLIDDIQFLQGKEQTMEEFFHTFNTL
                     HNANKQVVITSDLPPKQLNGFEDRMRSRFEWGLITDVQPPDLETRIAILRKKAGNERL
                     KAPDDVLEYIASRISTNIRELEGALIRVTAFANLNRQQVDRSLAEIVLKDLITDGTAA
                     EITSTAIIGQTAAYFGLSIEDLCGSSRSRVLVTARQIAMYLCRELTELSLPKIGQAFG
                     GRDHTTVMHANRKIRELMAERRSIYNQVTELTNRIKQQNRG"
BASE COUNT          790 a         1482 c         1312 g          624 t
ORIGIN      
        1 atgcccttgg cggccgcctt cttggcctgg cgcgccttga gcgccagctc ggccgcagag
       61 ccgggagccg gcatccgacg gatcgtgtag aactgctgac ccatcgacca caggttggtc
      121 gtcgtccagt agatcaggac accgatcggg aagttgatgc cggagaacgc gaagatcaac
      181 ggcatgaagt agagcagcat cttctgctgc tgcgccatcg ggccctcgag cgccgcgggc
      241 ggcatgttct tcatcgtcag ctggcgctgc gtgaggaacg tcgtcagcga catcgcgatg
      301 atgaggacga ccgtcacgat cttgacggtc gccccgccgt ccttcgccat gaagtagccc
      361 gacagcggtg caccgaagat ggtggcgtgc tccacctggc cggcgatggt ggcgtcgagc
      421 gggccgatgg tcgacttggc gtaggtaccc gtcgagagct tcggcagcga gtacagcacc
      481 cggaagagcg cgaagaagat gggcgactgc gcgaggatgg gcaggcacga cgcgaacggg
      541 ttcgttccgt gcttcttgta cagctccatc gtctcgcggc tcatcgcctc gcgggacgcg
      601 gggtcggact tgcccttgta cctcttctgg atcgcctgca tctcggggct gagcatctgc
      661 atgccgcggg acgccttgat ctgcttgacg aacagcggga tcagcacgat ccggatgacg
      721 atcacgagcc cgacgatcga cagcccccac gcagcgccgg aggccgggtc caggccgatc
      781 gccgtgagca acgtgtggaa ctggaccatg atccaggcca ccgcgaccat gatcgggtag
      841 agcaggccgt caaaccactc catgtggcag agctcctcgt tcggtgatca ggacgtggcc
      901 gaggccacgt gatggtgatg acactgccgg tcgtgagcag gcgggacgtc gtcgacgcca
      961 ccgatgttcc aggggttgca gcgcagcaga cgccacgccg cgagcccggt cccccggatc
     1021 gcgccatgcc gggtgacggc gatcacggcg tactgcgagc acgaagggta gaaccggcag
     1081 gtcggtggtc tcatcgggga gatgtacagc tggtaaccac ggatgagcac gagcagcaca
     1141 cggcccggga accggaccac cgcccgcgcg accgagccca tcgagacctt ggtcatggga
     1201 tgactgctca tccccgcacc gcacgccgcg cccgacgggc ggctgtctcg agcgccgtgt
     1261 ccagatccgc gccgaggtcg gcatagcccc gcgtcgcggc aggagcgagt gcccgcacga
     1321 ccactcccgc gtcggacggc atgctgtcga gccgctcacg catgagagca cgcagacgac
     1381 gcttgacctt gttccgcgtc acggcgttcc cgatggactt ggacacaacg aaaccgacca
     1441 ccggaccagg tccggggtcg gtccgtgttg cgaggtgcac gacgagcgac tcgcgcccac
     1501 cacgcgcacc gcggcgcacc gcctgctcga actccgcagg gcggcgcatc cggtgcgctg
     1561 ctggcagcac cggcgcagac gtcaggccga gagctcttcg cgacccttgc gccgacgagc
     1621 ggcgaggatc gcgcggcctg cacgtgtgcg catgcgcaga cggaagccat gggtcttggc
     1681 ccggcgccgg ttgttcggct ggaacgtgcg cttggtcacg agtctctcca actacttcgg
     1741 ggcatcgatc gcgcacactt acgcgaccag gtctgagtgc cgagctcgac tgctccggat
     1801 cagcgcgcag cacgcgcgat tccggcgcca gggcgcgcca gcaggagcca tcaggaaggc
     1861 ttggcaacgc tacgccggac gagggtgtcg ggtcaaatgc tggacacctt gatgtcggcg
     1921 atcggcgcgc ctcgccgcta cgactgccgt caccgatcat cccatgatcc gcgtcggctg
     1981 tcgcgcaccg acacgcgccg ccgcgccacg tcacccgagc ggactatcgc agcaccccgt
     2041 caggacggct acgcttgccc gtcatcgccc tcttcgcggc gctgcgcggg ctcaaccact
     2101 cccacgcacc agccgcggtc caccggtcgt gccgtccata ccaccggtat gccggtcgcc
     2161 gcacacaggc tgtggacaaa ggtgtggatc gcgcacagcc gtgcgaaact cgaccacgcg
     2221 agcgacgacg ggtagtgagg gaaacagtgt catcacaaga cgatcagccg gcccgtgtct
     2281 gggcgcaggc ggtcgctgag ctcgagcaca gcccggacat cacgccccgg cagatcgcct
     2341 tcgtgaagct ggccaagccg ctgggcctcc tcgacggcac catgctcctg gcggtcggca
     2401 acgacctgac gaaggagtac ctcgagactc gcgtgcgcac cgaggtcacc gaggccctga
     2461 cctccgcgct cggccgcgag gcgcgcttcg ccatcaccat cgatgtcgac ctggcgtcgg
     2521 acgagccggc catctccgcg acgcggcccg ccgcaacgaa tgaggtggcc cgtccgcacg
     2581 acgcgccgag ccaccgcgac accgtgcatg acgacgacga cgggcactcc cacgactccc
     2641 agccgttccg cccggggtcc ggtcgcaggc cgtcgacccc gtcggaaccc gcccgcctga
     2701 acaagaacta ccagttcgag accttcgtca tcggctcgtc caaccgcttc gcgcacgcgg
     2761 cggcagtggc tgtcgccgag gcgcccgcca aggcctacaa cccgttgttc atctacggcg
     2821 actccggact cggcaagacg cacctgctcc atgccatcgg gcactacgcg cagaacctct
     2881 acccgtccgt gcgcgtccga tacgtgaact ccgaggagtt caccaacgac ttcatcaact
     2941 cgatccgcga cgacaaggcg ggtgcgttcc agcgccgcta ccgcgaggtc gacgtcctcc
     3001 tcatcgacga catccagttc ctgcagggca aggaacagac gatggaggag ttcttccaca
     3061 cgttcaacac cctgcacaac gcgaacaagc aggtcgtcat cacgtccgat ctaccgccga
     3121 agcagctcaa cggcttcgag gaccggatgc gctcgcgctt cgagtggggc ctcatcaccg
     3181 acgtccagcc cccggacctc gagacccgga tcgccatcct ccgcaagaag gccggcaacg
     3241 agcgcctcaa ggcacccgac gacgtgctcg agtacatcgc gtcgaggatc tcgaccaaca
     3301 tccgcgagct cgagggcgcc ctcatccgag tcactgcctt cgccaacctc aaccgtcagc
     3361 aggtcgaccg ttcgctcgcc gagatcgtgc tcaaggacct gatcaccgac ggcacggccg
     3421 cggagatcac ctcaacggcg atcatcggcc agaccgccgc gtacttcggc ctcagcatcg
     3481 aggacctgtg cgggtcgtcg cgctcacgtg tcctcgtgac cgcacggcag atcgcgatgt
     3541 acctctgccg tgagctcaca gagctctcgc tgccgaagat cggccaggcg ttcggcggtc
     3601 gagaccacac gaccgtcatg cacgccaacc gcaagatccg tgagctcatg gctgagcgac
     3661 gcagcatcta caaccaggtg accgagctga ccaaccggat caagcagcag aaccgcggct
     3721 gatcgggccg atccacccac cagcgccatc cgcaggcttc ccgacggcac atcacgtacg
     3781 ggtgtagtcg cgctcccccg cgccatcgat cagtcctctt cgccgcggcc cgacatctcg
     3841 gcgcaccaga gccgggacga ccggacatca tgcgacaagt ctcaccacaa gcacaggaca
     3901 tgtcccttga cggcgacctt cgacatccgg gtaaaacagc cgtgggacga atattcacac
     3961 ctgtgtacag acctgtggac ggcggtggac gacacgccgg tggcatgtgg acggatgaca
     4021 gctcttcggt ggacggcaga tgagcgcagg acgcccgtcc accggcccgg caacttgtcc
     4081 cccgcccgtg cacacccact gtccacagca gaaactcgcg atgacctgca gcgagacccg
     4141 tcgtccccag gattcacaaa ggcgaagacg actacgagag ttctctcact gggaaatcca
     4201 cacaccag
//
LOCUS       DASRKH010000166         4073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_50037, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000166 DASRKH010000000
VERSION     DASRKH010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50037"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1172)
                     /locus_tag="VFC06_02790"
     CDS             complement(<1..1172)
                     /locus_tag="VFC06_02790"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZL80860.1"
                     /translation="MLHELSITSLGVISSARLPLRPGLTVVTGETGAGKTMVLTGLGL
                     ILGGKATPDAVRVGAEEAVAEAIYDVPETSEGRALAVDAGAVFDEDGTVTVVRTVGST
                     TRSRSILGGRTVPQALLAQVGEELVTVHGQADQVRLRTPARQRQLLDAYAGPLHGEAL
                     AQYRTAWAAWREAADALARLLESEDLERARLKLLSDDLAALDEAAVQPGERAELEATG
                     KVLAHAEALRSLAVTAHDVLAGETEVTAAASLDAARRALDEAATHDPSLQALATRVAD
                     YLYGATDAAQELASYLDRLEAEPARLDAMHQRLSIISGAERRFRRAADELEPLRAGIA
                     EELGAGAVWEDRLEAARATHDDAKARMLQCAEKVTAGRHTAAERLAAHVQAELSLLA"
     gene            complement(1175..2020)
                     /locus_tag="VFC06_02795"
     CDS             complement(1175..2020)
                     /locus_tag="VFC06_02795"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HZL80861.1"
                     /translation="MTRRILIVANPHRPETLEAAVLARAELEQRGIEVCSDNRDGPID
                     AVMVFGGDGTMLRAAREARGTGAPLLGVNMGHVGFLAELERGDVAHAVNCIARGEFEV
                     EDRRTVEVVVRQPDGTVQSGWALNEAAIERSELLRTLDVAVEVDGHPLSSFGCDGVVI
                     STATGSTAHAFSGGGPVVWPDVEALLLVPLAAHALFARPLVVGPRSLFAVEVLERSPA
                     EGLVVLDGAETIAVARGGRIEVRLGTERVRLARTSDAPFTERLVRKFGLDTKGWRGAN
                     RAPKE"
     gene            complement(2017..2838)
                     /locus_tag="VFC06_02800"
     CDS             complement(2017..2838)
                     /locus_tag="VFC06_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017834276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HZL80862.1"
                     /translation="MRLDRAVYARGLARSRSHAQELISQGAVLVDGEPETKPSRIVAD
                     GVAIALAGAEDHYVSRAAHKLIGALDAFGALGLDVAGRRALDAGASTGGFTQVLLERG
                     VSHVTAVDVGHGQMSPLVAADPRVDVVEGLNVRDMEQGSPGSGADLVVADLSFISLTL
                     VIHALRTVAADHADLLLMVKPQFEVGKNGLDKHGVVVSSALHARAVTAVCESAQREGL
                     VVRAVERSPLPGPSGNVEFFVWFSQSWQARGAAQERVLAGHALSAKIAELVEGSP"
     gene            complement(2851..3021)
                     /locus_tag="VFC06_02805"
     CDS             complement(2851..3021)
                     /locus_tag="VFC06_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80863.1"
                     /translation="MSKFEEVREPLGEVHYPPEVEQALEAVSEVPEDLDEQAEFFDKI
                     QESLASRLREES"
     gene            complement(3023..4039)
                     /locus_tag="VFC06_02810"
     CDS             complement(3023..4039)
                     /locus_tag="VFC06_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HZL80864.1"
                     /translation="MPMPESMLKGTDGPLRDGFDAALVDLDGVAYRGLHAIPTAPGAL
                     QAARDAGMSLVFVTNNASREPHEVADHLTSLGIPTDADEVLTAAQAVARLMAADLEPG
                     ATVLAVGGKGLRSALTEYGFTLVASADDSPDAVVQGFVPEVSWRELAEAAYAVQAGAR
                     FFASNLDLTIPNERGIAPGNGSLVGTVVAATGVIPTAAGKPEPTMFHLAAASVASERP
                     LAIGDRLDTDLKGARAAGMPGLLVFTGVSSPADAVLAPRDQRPTYLGETLECLAEPHP
                     VPRREGATWSVGLASAWVDGRQLIVSGTGIDAVRAACAAAWAAADGGVQVDGGSIPDF
                     GIGF"
BASE COUNT          689 a         1444 c         1299 g          641 t
ORIGIN      
        1 gccagcaggg acaattccgc ctgaacgtgc gccgcgagcc tctcagcggc cgtgtgacgc
       61 cccgcagtga ccttctcggc acactgaagc atccgggctt tcgcgtcgtc gtgcgtcgcc
      121 ctggcggctt cgagacggtc ctcccagacc gcgccggcac caagctcttc ggcaatgccc
      181 gcgcgcaacg gctcgagctc atctgcggcc cgtcgaaacc ggcgctcggc tccactgatg
      241 atcgaaagcc tctggtgcat ggcgtcgagc cgcgccggtt cggcctccag acgatccagg
      301 taggacgcga gttcctgagc ggcatcggtg gcgccataca ggtagtcggc aacgcgggtc
      361 gcgagtgcct ggagtgaggg atcgtgcgtg gcggcctcgt cgagcgcgcg tcgcgccgca
      421 tcgagtgacg ctgcggcggt gacctcggtc tcccctgcca agacatcgtg cgccgtcacc
      481 gcgagcgaac gcagcgcctc ggcgtgcgcc aacaccttgc ccgtggcctc gagttcggcc
      541 ctttctcccg gttgcacggc ggcctcgtcg agcgcggcca ggtcatcgct cagcagcttc
      601 agcctggcgc gctccaagtc ctccgactcg agcaggcgcg ccaaggcgtc ggccgcctcc
      661 cgccacgccg cccacgccgt gcggtactgg gcgagcgctt cgccatggag tggccccgcg
      721 tacgcgtcca acagctggcg ctgacgcgct ggggtgcgca gccgcacctg atcggcctgg
      781 ccgtgaacgg tcacgagttc ttcgcccact tgagcgagga gcgcctgggg cacggtgcgc
      841 ccgccgagga tcgaccggga ccgggtcgtg gatccgacgg tgcgcacgac cgtgacagtt
      901 ccgtcctcgt cgaagaccgc ccctgcgtcg acggccaggg ctcgcccctc agacgtctct
      961 ggtacgtcat agatcgcctc ggcaacagcc tcttcggcgc ccacgcggac ggcgtcgggc
     1021 gtggccttgc ctcccaagat gaggcccaaa ccggtcagca ccatcgtctt gcccgcgccc
     1081 gtctcacccg tcaccacggt caacccgggg cgcaatggga ggcgcgcgga agagatcact
     1141 ccaagcgagg tgatcgacaa ctcgtgtaac acgcctactc cttgggcgcc cggttcgcgc
     1201 cgcgccagcc cttggtgtcc aagccgaact tgcgcaccag gcgctcggtg aacggtgcgt
     1261 cggacgttcg ggcgagcctg acacgctctg ttcccaggcg cacctcgatc cggccgccac
     1321 gggccacggc aatggtctcg gcgccgtcga gcaccaccag tccctcggcg ggcgagcgtt
     1381 ccagcacctc gacggcgaac agtgaacggg gccccacgac caaggggcgc gcgaacagcg
     1441 cgtgagccgc caagggcacc aacagcagcg cctcaacatc cggccacacc acggggccgc
     1501 ctccggagaa ggcgtgcgct gtggaaccgg tggcggtgga gatgacgacg ccgtcacagc
     1561 caaagctgga cagcgggtgg ccgtcgacct cgacggcaac atcgagagtt cgcagcagtt
     1621 cggagcgctc gatggcggcc tcgttaaggg cccaaccaga ttggaccgtg ccatcgggct
     1681 gacgcaccac tacctcgacg gtgcggcgat cctccacctc gaattcgccc ctggcgatgc
     1741 aattcacggc gtgcgcgaca tccccacgct caagttcggc gagaaagccc acgtggccca
     1801 tgttcacgcc cagcaacggc gcgccggtgc cgcgggcttc cctggcggcg cgcagcatcg
     1861 tcccgtcccc tccgaagacc atgacggcgt cgatcggtcc gtcacggttg tcggagcaca
     1921 cctcaatacc gcgctgctca agttccgcgc gtgcgagaac tgcggcctcg agtgtctcgg
     1981 gccgatgcgg gttcgcaaca atcaggatgc gacgggtcat ggacttcctt cgacgagctc
     2041 ggcaatcttg gcactcagcg catgcccggc gagcacgcgc tcttgcgccg cccccctagc
     2101 ctgccatgac tgactgaacc atacgaagaa ttccacattg ccacttggac caggcaacgg
     2161 gctgcgctcg acggctctga cgacgagtcc ctcgcgttgg gcgctttcgc agaccgcagt
     2221 gacggctcgg gcgtgcagtg ccgacgagac gacgaccccg tgcttgtcga ggccgttctt
     2281 tcccacttca aactgcggct tcaccatcag gagtaggtcc gcatgatccg ctgcgaccgt
     2341 gcgcaaggcg tgaatgacca gcgtcagcga gatgaacgag aggtccgcga ccacaaggtc
     2401 ggcccccgag ccgggagagc cctgctccat gtctcggacg ttcaggccct cgaccacgtc
     2461 gaccctaggg tcggcagcaa caagaggtga catctgccca tgccccacat cgaccgcggt
     2521 gacgtgactg actccgcgtt ccaggagcac ttgggtgaat ccgcccgttg aggcgccggc
     2581 gtcaagcgca cgcctgccgg ccacgtcgag gccgagcgcg ccgaaagcgt cgagtgctcc
     2641 gatgagtttg tgggcggcac gagaaacata gtgatcttca gcgccagcca gtgcgatggc
     2701 gacaccatcc gcgacaatgc gactcggctt ggtctcgggc tcaccgtcga cgagcacggc
     2761 gccctgcgaa atcaactctt gggcatgcga ccgcgatcgc gcgagcccgc gcgcgtagac
     2821 ggctcggtca agccgcatcg gcggcgaggc tcaggattcc tcgcgcaagc gcgaggcgag
     2881 ggactcttgg atcttgtcga agaactccgc ctgctcgtcc aggtcctccg ggacctcgct
     2941 gacggcctct agcgcctgtt ccacctcggg gggatagtgc acctcaccca atggctcgcg
     3001 gacttcctcg aacttgctca tgtcaaaacc ctatcccgaa gtcaggaatg cttccaccgt
     3061 caacctgtac cccgccatcg gcagctgccc aggccgcagc gcacgcggca cggacggcgt
     3121 caatgccggt gccggagacg atcagttgtc ggccatccac ccacgccgat gccaaaccta
     3181 cgctccacgt cgcgccttcc cggcgcggga ctgggtgtgg ttctgccagg cattcgaggg
     3241 tttcgccgag atatgtgggg cgctgatccc tgggcgcaag caccgcgtct gcaggagagg
     3301 acactccggt gaagacaagg agccccggca tcccggccgc cctcgcgccc ttgaggtctg
     3361 tgtcgagccg gtcgccgatg gcgagcggcc tctcggaggc aaccgacgcg gccgcaaggt
     3421 gaaacatcgt gggctccggc ttgccggccg cggtggggat caccccggtc gcggcgacga
     3481 cggtgcccac caaggagccg tttccgggtg caatgccccg ctcattggga atcgtcaggt
     3541 cgaggtttga ggcaaagaac ctggcgcctg cctgcaccgc ataggcggct tcagcaagct
     3601 ctcgccacga tacttcgggg acgaaaccct gcaccacggc gtcgggactg tcatcagcgg
     3661 acgcgaccag tgtgaacccg tactcggtca atgcggagcg caatcccttg cctcccacag
     3721 ccagcaccgt ggcgccgggc tcaaggtctg cggccatcag gcgcgccacc gcctgcgccg
     3781 cggtcagcac ctcgtccgcg tccgtgggga tgccaaggct ggtgagatgg tctgccacct
     3841 cgtgcggttc acggctcgcg ttgttcgtca caaacacgag gctcatgccg gcgtcacgcg
     3901 cagcctgcag ggcccccggc gccgtcggga ttgcatgaag gccgcggtaa gcgacgccgt
     3961 ccaagtccac caaggcggcg tcaaaaccat cccgcaacgg gccatcggtg cccttcaaca
     4021 tcgactcagg catcggcatc gtcctctagg tcatagacaa taacttcctc ttc
//
LOCUS       DASRKH010000167         2498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_260, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000167 DASRKH010000000
VERSION     DASRKH010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2151
                     /locus_tag="VFC06_02815"
     CDS             <1..2151
                     /locus_tag="VFC06_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HZL80865.1"
                     /translation="QVTNPPLDAIREEIVTAIGGAIGPEPNLLAATPEHARKVILDFP
                     VIDNDELAKIIHIDADPKLRGVFSSRRIQGLYNVHNGGTGLDARLEEIFVEVDRALED
                     GVSFLILSDRDSDADHAPIPSLLLTSAVHHHLVRHHNRTQLSLVVEAGDVREVHHVAL
                     LVGYGVGAVNPYLAMETVEDLVSRGYITDITADQAVKNLIKALGKGVLKIMSKMGIST
                     IASYRGAQVFEAVGLSRELVNRHFAGTPSQIDGVGLDVIAREVGQRHADAYPASGQRP
                     AHRRLNSGGEYQWRRDGEEHLFDPETVFKLQHSTRTRQYTVFNEYAERVNNQSKRLMT
                     LRGLLDFASDREPIPIEEVEHIRDIVKRFTTGAMSFGSISQEAHETLAVAMNKLGGKS
                     NTGEGGESPERLYDPKRRSAIKQVASGRFGVTSEYLVNADDLQIKLAQGAKPGEGGQL
                     PGAKVYPWIAETRHSTPGVGLISPPPHHDIYSIEDLKQLIHDLKNANPKARVHTKLVS
                     EIGVGTIAAGVAKCKSDVVLISGSDGGTGASPLNSLKHAGAPWEIGLADTQQTLVRND
                     LRDRIVVQVDGQLKTGRDVVMATLLGAEEFGFATAPLVVSGCVMMRVCHKDTCPVGVA
                     TQNPELRDRFTGKPEFVETFFEFIAQQVRELLARLGFRSIEEAVGHVEMLDTRAAIDH
                     WKAKGLDLAPILAVPPAGANLHQSSAARVSNIST"
     gene            complement(2043..>2498)
                     /locus_tag="VFC06_02820"
     CDS             complement(2043..>2498)
                     /locus_tag="VFC06_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015936893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HZL80866.1"
                     /translation="GLADTQQTLVRNDLRDRIVVQVDGQLKTGRDVVMATLLGAEEFG
                     FATAPLVVSGCVMMRVCHKDTCPVGVATQNPELRDRFTGKPEFVETFFEFIAQQVREL
                     LARLGFRSIEEAVGHVEMLDTRAALDWCRFAPAGGTARMGARSSPFAFQ"
BASE COUNT          467 a          826 c          793 g          412 t
ORIGIN      
        1 caagtgacga acccgcccct tgacgcgatc cgcgaagaaa tcgtcaccgc catcgggggt
       61 gccatcggcc ccgagccgaa cctgctcgca gcgacgcccg agcacgcccg caaggtcatc
      121 ctcgactttc cggtgatcga caacgacgag ctcgccaaga tcattcacat cgacgcggac
      181 cccaagttgc ggggcgtgtt ctcttcgcgc cgcattcaag gcctctacaa cgtgcacaac
      241 ggcgggaccg ggcttgacgc gcgcctcgag gagatctttg tcgaagtgga tcgtgcgctg
      301 gaggacggtg tcagcttcct gatcctgtcc gaccgcgact cggacgcgga ccacgcgccg
      361 attccgtcgc tgttgctgac gtccgccgtg caccaccacc tggtgcgcca ccacaaccgg
      421 acgcagttgt ctctggtcgt ggaggccggg gacgtgcgcg aggtgcacca cgtcgcgctg
      481 ctggtcggct acggcgtggg cgcagtgaac ccctaccttg cgatggagac cgtcgaggat
      541 cttgtgagcc gcgggtacat caccgacatc accgcggacc aggccgtcaa gaacttgatc
      601 aaggcgctcg gcaagggcgt cctgaagatc atgtcgaaga tgggaatctc gacgatcgcc
      661 tcgtaccgtg gtgctcaggt cttcgaggca gtgggtctgt cgcgagagct ggtcaacagg
      721 cacttcgccg gcacacccag ccagatcgac ggagtgggcc tcgacgtcat cgcacgcgaa
      781 gtggggcagc gtcacgccga cgcctatccc gcatcgggcc agcgtcccgc ccaccgtcgc
      841 ctcaactcgg gtggtgaata ccagtggcgc cgcgacggcg aggagcactt gttcgacccc
      901 gagacggtat tcaagcttca gcattcgacg cgcacgcgcc aatacaccgt cttcaacgag
      961 tacgcggagc gcgtcaacaa ccagtccaag cgcctcatga cgctgcgtgg actccttgac
     1021 ttcgcgtcgg atcgggagcc cattccgatc gaggaagtgg aacacatccg cgacatcgtc
     1081 aagcgcttca ccacgggcgc catgtcgttc ggatcgatct cgcaagaagc gcacgagacg
     1141 ctggccgtcg cgatgaacaa gttgggcgga aagtccaaca ccggcgaagg gggagagtct
     1201 cccgagcggc tgtacgaccc caagcgccgc agtgccatca agcaggtggc ctcgggccgg
     1261 tttggcgtca ccagcgagta cctagtgaac gccgacgacc tccagatcaa gcttgcccaa
     1321 ggcgcgaagc ccggcgaggg tggacagctt cccggcgcga aggtgtaccc gtggatcgcc
     1381 gagacgcgtc actccacgcc tggcgtgggc ctcatctctc cgccgccgca ccacgacatc
     1441 tactcgatcg aggatctcaa gcagttgatc cacgacctga agaacgcaaa ccccaaggca
     1501 cgggtgcaca ccaagctcgt ctccgagatc ggtgtcggga cgatcgccgc aggtgtggcc
     1561 aaatgcaagt cggacgtggt gctcatctcc ggttcggacg gcggcaccgg cgcgagcccg
     1621 cttaactcgc tcaagcacgc gggcgcaccg tgggagatcg gcctggccga cacccagcag
     1681 acgctcgtcc gcaacgactt gcgcgaccgt atcgtcgtgc aggtcgacgg gcagttgaag
     1741 accgggcgcg acgtggtcat ggcgacgttg ctcggcgccg aggagttcgg ctttgcgacc
     1801 gctccgctgg tggtctccgg ttgcgtgatg atgcgcgtgt gtcacaaaga cacgtgcccc
     1861 gtcggcgtcg ccacccagaa ccccgagctg cgcgaccgct ttaccggcaa gccggagttt
     1921 gtggagacgt tcttcgagtt catcgcccag caggtgcgcg aactgcttgc tcgcctgggc
     1981 ttccggtcca ttgaagaggc ggtgggtcac gtcgagatgt tggacacccg cgcggcgatc
     2041 gatcactgga aggcgaaggg actggacctc gcgcccatcc tcgccgtccc gcctgccgga
     2101 gcgaacctgc accagtcgag cgccgcgcgg gtgtccaaca tctcgacgtg acccaccgcc
     2161 tcttcaatgg accggaagcc caagcgagca agcagttctc gcacctgctg ggcgatgaac
     2221 tcgaagaacg tctctacgaa ctccggcttg ccggtgaagc ggtcgcgcaa ctcggggttc
     2281 tgggtggcga cgccgacggg gcacgtgtcc ttgtgacaca cgcgcatcat gacgcaaccg
     2341 gagaccacca gcggagcggt cgcgaagccg aactcctcgg cgccgagcag tgtcgccatg
     2401 accacgtcgc gtccagtctt caactgtccg tcgacctgga cgactatgcg gtctcgcaag
     2461 tcgttgcgga cgagcgtctg ttgggtgtcg gcaagtcc
//
LOCUS       DASRKH010000168         2030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_45903, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000168 DASRKH010000000
VERSION     DASRKH010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45903"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="VFC06_02825"
     CDS             <1..593
                     /locus_tag="VFC06_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018763797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZL80867.1"
                     /translation="PDLDIVPVFGDISKQEDVDRIVAAAGERIDCLANNAGIMDNFTP
                     IHECSDAVWERVFRINVTGMFMLTRAVVPFMLKQGAGSIVNISSEAGLRASAAGAAYT
                     ASKHAVIGLTKSGAFMYKNAGIRVNSVAPGAVKTNIEAPFESAFSGPIIQKAMANIPT
                     FAEASHLASAICYLLSDDAVNLTGVILPSDGGWSVV"
     gene            complement(656..1324)
                     /locus_tag="VFC06_02830"
     CDS             complement(656..1324)
                     /locus_tag="VFC06_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HZL80868.1"
                     /translation="MAVSSDDGPPPTPGQGVGPWPGGSDAWPDGDHWDPELLEHGDAR
                     NVVDRYRYWRMDAIVADIDRRRHPLHVAIENFTHDSNIGSVVRTANAFAVAEVHIVGR
                     RRWNRRGAMVTDRYQHLRHHDTVADFLAWAGEAGMIVIGIDNIPGSEPLEGETLPRET
                     VMVFGQESAGLSEEMVAGCERVLHITQYGSTRSMNVGAAAAIAMWSWVVQYAGPPGEG
                     EVSR"
     gene            complement(1325..1924)
                     /locus_tag="VFC06_02835"
     CDS             complement(1325..1924)
                     /locus_tag="VFC06_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HZL80869.1"
                     /translation="MDPIMTTLVIVGVLLVAVVLWFIATYNAFVRLRNLVEEAWRQVD
                     VELHRRYDLIPNLVETVKGYASHERGVFEEVTLARAAAAGGAPTREQQAANENALTAA
                     IGRLFAVAEAYPDLKANANFQALQAELTNTEDRIAAGRRYYNANVRQFNTKLETFPAS
                     IVGNMTGFRRATYFEAEGTSRDVPRVDFGQGGGSGAPRP"
BASE COUNT          311 a          718 c          681 g          320 t
ORIGIN      
        1 accccgacct cgacatcgtc cccgtgttcg gtgacatctc gaagcaggag gacgtggaca
       61 ggatcgtcgc cgccgccggg gagcgcatcg actgcctggc caacaacgcg ggcatcatgg
      121 acaatttcac ccccatccac gagtgcagcg acgcggtctg ggagcgtgtg ttccgcatca
      181 acgtcacggg catgttcatg ctcacccgcg cggtcgtccc gttcatgctc aagcagggtg
      241 ccggctccat cgtcaacatc tcgtccgagg ccggactgcg cgcctcggcc gccggcgcgg
      301 cgtacaccgc gtccaagcac gccgtcatcg gcctcaccaa gagcggcgcg ttcatgtaca
      361 agaacgccgg catcagggtg aactcggtgg cgccgggcgc ggtgaagacg aacatcgagg
      421 caccgttcga gtccgcgttc agcggcccga tcatccagaa ggcgatggcg aacatcccga
      481 ccttcgccga ggcctcgcac ctcgcctccg ccatctgcta cctgctcagc gatgacgcgg
      541 tgaacctcac cggcgtcatc ctgccctccg acggcggctg gtccgtggtc tgaccgggag
      601 ggatgacgcc gagggggagg tttcacaccc acggtgcggc gcgcgccacg cggtctcagc
      661 gcgaaacctc cccctcgccg ggcggcccgg catactgcac cacccaggac cacatcgcga
      721 tcgcggccgc cgcgcccacg ttcatcgagc gggtggagcc gtactgggtg atgtggagaa
      781 cccgctcgca ccccgccacc atctcctcgg acaaccccgc cgactcctgg ccgaacacca
      841 tcacggtctc gcgcgggagc gtctccccct caaggggctc cgatccggga atgttgtcga
      901 tcccgatcac gatcatgccg gcctcccccg cccacgcgag gaagtcggcc acggtgtcgt
      961 ggtgtcggag gtgctggtaa cggtcggtga ccatcgcgcc gcgccggttc cagcgccgtc
     1021 ggcccacgat gtgcacctcc gccacggcga acgcgttggc ggtgcgcacc acggacccga
     1081 tgttcgagtc gtgcgtgaag ttctcgatcg ccacgtgcag cgggtggcgg cgccggtcga
     1141 tgtccgcaac gatcgcgtcc atccgccagt agcggtaccg gtcgacgacg ttccgggcgt
     1201 cgccgtgttc gagcagttcc gggtcccagt ggtcaccatc cggccaggcg tcggaccctc
     1261 ccggccacgg cccgacgccc tgcccggggg tggggggcgg gccgtcgtcg gaggaaacgg
     1321 ccacctacgg ccgcggagcc ccggagccgc cgccctggcc gaagtccacg cgggggacgt
     1381 cgcgcgacgt gccctcggcc tcgaagtagg tggcgcggcg gaagccggtc atgttgccca
     1441 cgatcgaggc ggggaaggtc tcgagcttcg tgttgaactg gcgcacgttg gcgttgtagt
     1501 agcgccgtcc tgccgcgatc cggtcctcgg tgttggtcag ttcggcctgc agggcctgga
     1561 agttggcgtt cgccttcagg tccgggtagg cctcggcgac ggcgaacagc cgcccgatcg
     1621 cggcggtcag cgcgttctcg ttggcggcct gctgttcacg ggtgggcgct ccaccggcgg
     1681 ccgccgcgcg cgcgagcgtg acctcctcga agacaccacg ctcgtggctg gcgtagccct
     1741 tgacggtctc caccaggttg gggatgaggt cgtagcggcg gtggagttcg acgtccacct
     1801 ggcgccacgc ctcctccacc aggttccgca gccggacgaa ggcgttgtac gtggcgatga
     1861 accagagcac cactgccacg agcagcacac ccacgatgac aagcgtggtc atgatcgggt
     1921 ccatgtcgaa tcctcccggg agttcaatgg tggcttcatc caaccacagc ggcgccgtcc
     1981 ggcgactgcc ggccggtgga agaacgtgtc gctacagccc gaggtccgtg
//
LOCUS       DASRKH010000169         2211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_4468, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000169 DASRKH010000000
VERSION     DASRKH010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4468"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2052)
                     /locus_tag="VFC06_02840"
     CDS             complement(<1..2052)
                     /locus_tag="VFC06_02840"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HZL80870.1"
                     /translation="MWCANGHERVEYEQRVVALTEGSNICPACGYGAQVRYTHPDLSA
                     RLVREEDRNCSAASLVRVLVTCADGHERVEYEQPLLRLRDGHVACPACKRLKTIAVTH
                     PDLALRIVHPEDRRRGSRTPTKVLVSCANGHPEERYMQPVCNLTGGQNGCPVCGMHSI
                     AGARHRERLAEIVEGILESIDELSDAELVHICQRWRLNNVKGGLGQALLSRNVETVRR
                     TLADLTGGEEPNGAAVESDGLDETVFTVAATTDTDAEDDALADEAGQPAHADGSEVTP
                     ERFLAATDTAVRLSHRDGPDNVRFLIDSRVAHAWRRVFAGDGMGEFVDGLREAELNEF
                     TRIVVETFLRQYDAAVEMAMPSGWQFRPERDAEVVQPNLMQRHLACLVAEQRQVGNWS
                     STGAGKTVSAVLAARHIEARTVVVICPNGTVEGWARTAQSCFADNQVATRTLSPTWEG
                     SGPRWVIINFDMLPGHEGEAQALIDSLDPDMLVVDEVHWAKDRRGTPTSQRREVLMGI
                     RRRLRADAAVLGMSATPVVNDLSEGASLIELIDGVRPNVDTRRVTITNVISLHEHMLR
                     VGFRAIIDPGVGLDVSHPEIDLGSDVADSIANRGGSHPAGIDMILAEAYREHVVATAL
                     DCRARGRGLVIYSEYVQDIVDPLREALRSVGLTVALYTGAEKTTIADLTSSGVDVLIG
                     TS"
BASE COUNT          379 a          755 c          689 g          388 t
ORIGIN      
        1 ggacgtgccg atgagcacat cgaccccgct cgaagtcagg tcggcgatgg tcgtcttctc
       61 ggcccccgtg tacagggcga ccgtcagacc gacggaacgc aacgcctccc ggagcgggtc
      121 gacgatgtcc tgcacgtact ccgagtagat gacaaggccg cgcccgcgag cgcgacagtc
      181 gagtgcggtc gccacgacgt gctccctgta cgcctccgcg aggatcatgt cgatgcctgc
      241 ggggtgtgag ccgcctctgt tggcgatgct gtcggcaacg tcgctgccaa ggtcaatctc
      301 ggggtgtgac acgtccaggc cgacgcccgg gtcgatgatg gcacggaagc cgactcgaag
      361 catgtgctcg tgaagcgaga tgacgttggt gatcgtgacc ctgcgggtgt ctacgttcgg
      421 acgcactccg tcgatcaact cgatcaggga ggcaccttcg gacaggtcgt tcacgaccgg
      481 tgtggctgac attccgagca ctgcggcgtc agcgcgcagt cggcgcctga tacccatcag
      541 cacttcgcgg cgttgcgacg taggtgtgcc gcgacgatcc ttcgcccagt gcacctcatc
      601 gacgacgagc atgtccgggt ccagactgtc gatgagagcc tgagcctcac cctcatgacc
      661 gggcagcatg tcgaagttga tgatgaccca tcgggggccg gagccctccc acgtgggaga
      721 cagcgtgcgc gtcgccactt ggttgtcagc gaagcaggac tgcgcagtgc gcgcccaccc
      781 ttcgacagtc ccgttcgggc agatgacgac gacagtgcgc gcctcgatgt gacgtgcggc
      841 gagcaccgct gagaccgtct tgccggcacc agtagacgac cagttgccca cttgtcgctg
      901 ctcggcgacc aggcacgcga gatgccgctg catcaggttc ggttgcacca cctcggcgtc
      961 acgctcggga cggaactgcc agccggacgg catcgccatc tccacggcgg cgtcgtactg
     1021 gcgcaagaac gtctcgacga cgatgcgggt gaactcgttg agttccgcct cgcgcagacc
     1081 gtcgacgaac tcgcccatac cgtccccggc gaacacccgc ctccacgcgt gagcgactcg
     1141 tgagtcgatg aggaacctca cgttgtcggg gccatcacga tgggacaggc gcaccgccgt
     1201 gtcggtcgca gcgaggaacc gctccggtgt cacctccgaa ccgtcagcgt gcgcaggctg
     1261 tccggcttcg tcggcgagag cgtcgtcctc cgcgtccgtg tccgtagtgg cggccacagt
     1321 gaacaccgtc tcgtcgagac cgtcggactc gacggcggca ccgttcggct cctcaccacc
     1381 cgtcaggtcg gccaacgtcc gccgcaccgt ttcgacgttg cgagacagca gcgcctgccc
     1441 gagcccgccc ttgacgttgt tcagacgcca cctctgacag atgtgcacca gttcggcatc
     1501 cgacagttcg tcgatggatt cgagaatccc ctccacgatc tctgccagac gctcccggtg
     1561 cctcgcgccc gcaatgctat gcatgccaca taccgggcaa ccattttgac cgccagtcag
     1621 gttgcacacc ggctgcatgt accgctcttc ggggtgccca ttggcgcacg acacgagcac
     1681 cttggtcggg gtgcgcgacc cacgacgacg gtcctcagga tgcacgatgc gcagcgccag
     1741 gtccgggtgc gtcactgcga tggtcttgag acgcttgcat gccgggcacg ccacgtgccc
     1801 gtcccgtagg cggagcaacg gctgctcgta ctcgacgcgc tcgtgaccgt cggcgcatgt
     1861 cacgagaacg cggacgagcg atgctgccga gcagttccta tcctcctctc gtaccagccg
     1921 agcgctcaag tcggggtgcg tgtaccgcac ttgtgcgcca tacccgcacg ccggacaaat
     1981 gttggagccc tcagtgaggg ccacaacccg ctgctcgtac tcgacacgct cgtgaccgtt
     2041 ggcgcaccac acgagaacct tcttaggtga acgcagcgga acgttgcgtt tcgattggtc
     2101 gaccaggcgt gctgcgaggt cgggccgcgc cttgcccaac ttcgcccagt acccacatgc
     2161 cgggcaagtg ttcgccccct cagtcaactt gccgaccgct tgcatatact c
//
LOCUS       DASRKH010000170         2597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_27759, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000170 DASRKH010000000
VERSION     DASRKH010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27759"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..283
                     /locus_tag="VFC06_02845"
     CDS             44..283
                     /locus_tag="VFC06_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80871.1"
                     /translation="MESGTWRLVVDGECFDVVERTPGVYDLTWLTGPNADYGFTAAAS
                     DGKPMGRVQLEEHARVFLRQVDPQTGHVEAGETLR"
     gene            complement(348..644)
                     /locus_tag="VFC06_02850"
                     /pseudo
     CDS             complement(348..644)
                     /locus_tag="VFC06_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612271.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            720..951
                     /locus_tag="VFC06_02855"
                     /pseudo
     CDS             720..951
                     /locus_tag="VFC06_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007053875.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1199..2095
                     /locus_tag="VFC06_02860"
     CDS             1199..2095
                     /locus_tag="VFC06_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZL80872.1"
                     /translation="MVQRNTLIHRLGRDRIASAGAVTVAVAVVAAVFAPVLAGLSGND
                     PYAYDLSALDATGAPAGWGGGISAAHWFGVEPLTGRDLFSIVVHGARTSLLVGVGASV
                     VAVMLGVLVGITAGYFGGWFDVAASRVIDVLFGFPGMIFMIAVGAIAPAAFPRVLLLV
                     LVIGFFGWPSIARVVRGQTLALRTQGYVRASVALGAGPGHVLLRQILPGLTATIVVFS
                     TMSIPGTIGAEAALSFLGVGVPPPTPSWGRSIGDAVTWFSTDPFYLLFPGVALFVVTL
                     AFNVFGDGLRDALDPRSSGLTR"
     gene            2140..>2597
                     /locus_tag="VFC06_02865"
     CDS             2140..>2597
                     /locus_tag="VFC06_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZL80873.1"
                     /translation="MARADVVHTVRFVASKVAGLALVLLIVSALTFAVFVLLPADPAQ
                     LSCGRPCTPQRLEAARGYMGLDRSAWNQYADFLRGIVAGRTFGAGPTAITCDAPCLGY
                     SFRLNESVTTLIVSRLPVTFSIAIGAAVLWFVGGVAAGVVAAIRRGTLTDR"
BASE COUNT          339 a          885 c          912 g          461 t
ORIGIN      
        1 agaacgagca aactgtcaca agaggacacc cgaaggaggc ggaatggagt cgggaacgtg
       61 gcgactggtc gtagacggtg agtgcttcga cgtcgtagag cgaacgccgg gcgtgtacga
      121 cctcacctgg ctgacggggc ccaacgcgga ctacggcttc accgcggccg cctccgacgg
      181 caagcccatg ggccgagtgc agctcgagga gcacgcccgg gtcttcctcc ggcaggtcga
      241 cccgcagacc gggcacgtgg aggcggggga aacactgcgc tagcctcgcg ccttcacagc
      301 cgagtggctc gaggccgtgt cgctgaactg caaagtgctc ctgacctgtt ggcaaggccg
      361 gtgtcgttgg cgatgcggat gcggtcctcg cagcgggccc ggcgccggtg gcgcagctcg
      421 aggtcgggca gctggccgcg gacggtgttg gtcgcgaacg cggtgatccg catcccctcg
      481 acgtcctcaa accgcagctg ggcgccgggg tggggtcgtt ccttgcgcac gatcacgcgc
      541 atgcccttgg gccagccggt caggtccatc aggtcggtca gctcggcgac ccaggcgccg
      601 tcgcggatgg cgtcgtgggc gtcgtaggcc ggtgtccaga cgtcgtcggg gaggggggcg
      661 cgggtagacc ctcctcgtgg cgcgcaacgc cgcaggtcag cggggggcct gactggttac
      721 gtctggatgg cgtcgaccag ctcgtcctgt cgttgacggc gcgcgggctg acgaccggtg
      781 aggtcgccgc gcacttcatg gacgtctacg gcgcgaacgt cccgagagac acgatctcca
      841 ggatcacgga gaaggtcgcc ggggagatgg tcgagtggaa gcaccggccc ctggaccgcg
      901 tctacccggc gatgcgtcat cgacgcgatc gtgctgaagg tccgcgacgg ctccaaagcc
      961 tgcgaagtcc ctcctcacat gcccagacac cacttgacga ggccacggtc ggtggtgacg
     1021 tatggtcagg gcattccgca tcaggctgtg tggcgcgacg ggcgcccgcg gatcggtctg
     1081 agctggcagg tagaggggaa tcgtcgtggt tgagaggtgt tgtcggtgct cgcgccgcca
     1141 ctctcacccg acgccgccgt aaaggacctg catgtcgctc gaccacacct cccctgggat
     1201 ggtgcagcgc aacaccctca tccatcgcct gggccgtgac cgcatcgcca gtgccggcgc
     1261 ggtgaccgtt gccgttgccg tcgtcgccgc agtgttcgcc cccgtgctgg cgggactgtc
     1321 cggcaatgat ccgtacgcct atgacctctc cgcgctcgac gcaaccgggg cgccagcggg
     1381 gtggggcggc ggcatcagcg ctgcgcactg gttcggcgtc gaaccgctca ctggtcgtga
     1441 cctgttctcg atcgtggtgc acggcgcgcg gacttccttg ctcgtcggtg tcggcgcgag
     1501 cgtggtagcc gtgatgctcg gagttctcgt cgggatcacg gccgggtact tcggcggatg
     1561 gttcgacgtc gcggcgagcc gggtcatcga cgtgctgttc gggttccccg gaatgatttt
     1621 catgatcgca gtgggcgcga tcgctccggc cgcattcccg cgggtcctcc tgctcgtgct
     1681 ggtgatcggg ttcttcggct ggccgtcgat cgcccgcgtc gttcgcggac agaccctcgc
     1741 gctgcgcacc caggggtacg tccgtgcctc cgtcgccctc ggcgccggcc cgggccacgt
     1801 cctgctgcgt cagatcctgc ccggcctgac agcgacgatt gtggtcttct cgacgatgag
     1861 cattcccggg acgatcggcg ccgaggctgc actgtccttc ctcggggttg gcgtcccgcc
     1921 gccaaccccg tcgtggggca ggtcgatcgg cgatgcggtg acctggttct cgacagaccc
     1981 gttctacctc ctgttccccg gtgtcgcgct gttcgtggtc acccttgcct tcaacgtgtt
     2041 cggcgacggg ctgcgcgacg cgctcgaccc ccgctcaagc gggctcacac gatgaccgcg
     2101 ctcgcgcggg tcccctccgc acctgcgtcc tcccgtggga tggcccgggc cgatgtcgtg
     2161 cacaccgttc gcttcgtggc gagcaaggtc gccgggctcg cgctcgtcct gctgatcgtc
     2221 tcggccctga ctttcgccgt gttcgtcctc cttccggccg atccggccca gctttcgtgc
     2281 ggccgcccgt gcacgccgca gcgactcgag gcagcacgcg ggtacatggg gctcgaccgg
     2341 tccgcgtgga accagtacgc ggacttcctg cgcgggatcg tcgccggacg caccttcggc
     2401 gccggcccga ctgcgatcac ctgcgacgcg ccctgcctcg ggtactcgtt ccggctgaac
     2461 gagtcagtga ccacgctgat cgtcagccgc ctgccggtga cgttctccat cgcgatcggc
     2521 gcggcggtgc tgtggttcgt cggcggagtc gccgccgggg tggtcgccgc catccgccgc
     2581 gggactctca ccgaccg
//
LOCUS       DASRKH010000171         2096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_36016, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000171 DASRKH010000000
VERSION     DASRKH010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36016"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VFC06_02870"
     CDS             <1..181
                     /locus_tag="VFC06_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZL80874.1"
                     /translation="SSISDTFGVSTARASQHLQLLARAGLVEVTVDGPWRWYNLPRSP
                     GGQMVDWLRRLERSY"
     gene            complement(175..1473)
                     /locus_tag="VFC06_02875"
     CDS             complement(175..1473)
                     /locus_tag="VFC06_02875"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HZL80875.1"
                     /translation="MPGIVIVGAQWGDEGKGKATDLLGSRVDYVVKFNGGNNAGHTVV
                     IGDQKFALHLLPSGILTPGVIPVIGNGVVVDIGVLFHELDELLERGVDPSKLLISNAA
                     HVIAPYARTLDQVSERFLGKRKIGTTGRGIGPAYADKINRLGIRIGDLFDEAVLRDKV
                     EGALAAKNHLLVKVYNRRAIEVDEVVDELLGYRERLAPMVADTSLILNQALDAGKNVL
                     FEGGQATMLDVDHGTYPFVTSSNATAGGACTGSGVGPTKIDSVIGIVKAYTTRVGEGP
                     FPTELLDTWGERLRDVGHEFGTTTGRPRRCGWYDAVIARYASRINGLTDFVLTKLDVL
                     TGLDQIPVCVAYDVNGKRFDELPQDQADFAAAVPIYETFPGWTEDISKVKSFEELPQA
                     AQDYVKALEDISGCRMSAIGVGPERDQILVRHDLLHGRRQ"
     gene            complement(1580..>2096)
                     /locus_tag="VFC06_02880"
     CDS             complement(1580..>2096)
                     /locus_tag="VFC06_02880"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HZL80876.1"
                     /translation="EDGHSAEINDKLYSTPEDGIRTIEALGTGEKGRYLTALTFGNVH
                     GAYKPGAVKLRPEILGEIQAAAGAKVGKDKPFDLVFHGGSGSTAEEIALAVSHGVIKM
                     NVDTDAQYAYTRPVVDHMMKNYDGVLKIDGEWGNKKVYDPRAWGKAAEAGMAARVVQA
                     CQQLGSAGRKI"
BASE COUNT          335 a          709 c          662 g          390 t
ORIGIN      
        1 cagctcgatc agcgacacgt tcggcgtctc gaccgcacgg gcgtcgcaac atctacaact
       61 gctcgcgcgg gcggggctgg ttgaagtcac ggtcgacggc ccctggcggt ggtacaacct
      121 tccgcgctca cctggcggcc agatggtcga ctggctgcga agactcgaac gctcctactg
      181 acgccggccg tgcaggaggt cgtgcctcac cagaatctgg tcgcgttcgg gtcccacgcc
      241 aatggcagac atacggcagc cggagatatc ctcgagcgcc ttcacatagt cctgtgccgc
      301 ctgcggcaac tcctcgaagc tcttcacctt cgagatgtcc tcggtccagc cggggaacgt
      361 ctcgtagatc gggacggcgg ccgcaaagtc ggcctggtcc tgcgggagct cgtcaaagcg
      421 cttgccgttg acgtcgtatg ccacgcacac cggaatctgg tccaggccgg tcagcacgtc
      481 gagcttggtg agcacgaagt ccgtgagtcc gttgatgcgg gacgcatagc gagcaatcac
      541 ggcgtcgtac cagccgcaac ggcgcgggcg ccccgtcgtg gtgccaaact cgtggccaac
      601 gtcccgcagg cgttcgcccc acgtgtccag cagttccgtg gggaacggac cctcgcccac
      661 acgcgtcgtg tacgccttga cgatgccgat gacggagtcg atcttggtag gaccgacgcc
      721 cgagccggtg caggcccctc ccgccgtcgc gttcgatgac gtcacgaacg ggtaggtgcc
      781 gtggtcgacg tcgagcatcg tcgcctggcc gccctcgaac aggacattct tgccggcgtc
      841 gagcgcctgg ttgaggatca gcgacgtgtc ggccaccatc ggcgcgagcc gctcgcggta
      901 gcccagcaac tcgtcgacca cctcgtccac ctcgatcgcc cggcggttgt acaccttgac
      961 cagcaggtgg ttcttggccg ccagcgcgcc ctcgaccttg tcgcgcagca cggcctcgtc
     1021 aaagaggtcg ccgatgcgga tgcccaggcg attgatcttg tcggcgtacg ccgggccgat
     1081 gccgcggcct gtggttccga tcttgcgctt gccgaggaac cgctccgaca cctggtccag
     1141 cgtgcgcgcg tacggcgcga tcacgtgcgc cgcgttcgag atcagcagtt tcgaggggtc
     1201 gacgccgcgc tcgagcagtt cgtcgagttc atggaagagc acgccgatgt ccacgaccac
     1261 gccgttgccg atcaccggaa tgacgccggg cgtcagaatt ccggatggca gcaagtgcag
     1321 ggcgaacttc tggtccccga tcacaacggt gtgtccggcg ttgtttccgc cattgaactt
     1381 cacgacgtag tcgacgcgag agccaagaag gtcggtggcc ttccccttgc cctcgtcgcc
     1441 ccactgggct cctacgatca cgatgcctgg catcgcgcac tcccctcacc cagtcgaagc
     1501 cggggcttgg ttgctaccgg aacatgcgaa cgcccccggt gtctgacgac tccgggggcg
     1561 ttccgcgaaa tcgttgtacc tagatcttcc taccagcgga gccaagctgc tggcatgcct
     1621 ggaccacgcg ggccgccatg cctgcctcgg ccgccttgcc ccatgcacgg gggtcgtaga
     1681 ccttcttgtt gccccactcg ccgtcgatct tcagaacgcc atcgtagttc ttcatcatgt
     1741 ggtcgacgac gggacgcgtg tacgcgtact gcgcgtcggt gtcgacgttc atcttgatga
     1801 caccgtgcga gacggccagt gcgatctctt cggccgtgga cccggagcca ccgtggaaca
     1861 cgaggtcgaa cggcttgtcc ttgccgacct tcgcgccggc agcggcctgg atctcgccca
     1921 ggatctccgg gcgcagcttc acagcaccag gcttgtacgc gccgtgaacg ttgccgaacg
     1981 tgagggccgt caagtagcgg cccttctcgc cggtaccgag agcctcgatg gtccggatgc
     2041 cgtcttcggg ggtcgagtac agcttgtcgt tgatctcggc cgagtgtccg tcttcc
//
LOCUS       DASRKH010000172         2053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_46401, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000172 DASRKH010000000
VERSION     DASRKH010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46401"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            313..1932
                     /locus_tag="VFC06_02885"
     CDS             313..1932
                     /locus_tag="VFC06_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80877.1"
                     /translation="MTLPVTVSDVVAEHVTFEVECIDRMYLNVYVPGLQYPAGLVGYV
                     HHQLGLPIASTAPLGKITERFVAAVHRFARDQQVPWVDFAKGQRKDDVMHEYQRRFVA
                     AGRTEGVLFVGRAQEKTNLFRTEKRHNAEGRPYPWIVRASGVVNHFYFYCVDADFGPF
                     FLKFCSYFPYNARLCINGNHWAQRQAGKAGLGFVPMDNAFAAVDDPATLQAICDRLGP
                     EQIDALLRKWLAILPHPFSPADRAAGYRYDISILQAEFSLTQMLDRPVTGRIFFEQVI
                     RDNLDVGRPDHVSLIFDRRIVAKGKHPTPGRFRTRVITTGVTPSLHVDYKHTTIKQYH
                     KEGRALRTETTINNTRDFGIGKRLTNLPALREVGFTANRRLLRVERLDHDPTTGTAAL
                     TAVTEPVTTPTGTRVPGLRLGAQRSHALLSALLIFRLQPDGFTNRDLRALTAELRGLP
                     PEHVTTGQMTYDLRRLRVHGLIERIPHTHRYHLTETGLRTATFLTRVHDRYLPTGLAH
                     LNGPGDNRLRRAQTAYQHALEDLARQAGLPA"
BASE COUNT          360 a          764 c          611 g          318 t
ORIGIN      
        1 ggcgtcagga gccgggatct tctctaccgc cctggccaag gcgaggtcga cagggccacg
       61 cagccccgcc gggagggcgt cagagtcagt ggtcggcatc agggctaacc tccctcaagg
      121 gcccgttcgt gttcttcgag catcgcaaga tctaacgggg cttcgcccca gcccgacgag
      181 tcatggcgcg tggtgccctt tgggtgggct ccggccggtg ggatgacggt cgccgcggac
      241 ggcgccgtgt tgtaaccgcc ctgtggcggt ggttggctga catgagccgt tccacctgac
      301 cggagcccga tcatgacgtt acctgtcacc gtgtccgatg tcgtggccga gcacgtcact
      361 ttcgaggtgg agtgcatcga ccggatgtac ttgaacgtgt acgtgcccgg actgcagtat
      421 ccggccgggc tggtcgggta tgtccaccac cagttggggc tgccgatcgc ctcgaccgcg
      481 ccgctgggca agatcaccga gcggttcgtt gccgcggtcc accgtttcgc ccgggaccag
      541 caggtgccgt gggtcgactt cgccaagggt cagcgcaagg acgacgtgat gcacgaatac
      601 cagaggaggt tcgtggccgc tggtcgaacc gagggtgtgc tgttcgtcgg ccgggcgcag
      661 gagaagacca acctgttccg caccgagaag cggcacaacg ccgagggccg gccctacccg
      721 tggatcgtgc gggccagcgg ggtggtcaac cacttctact tctactgcgt ggacgctgac
      781 ttcggcccgt tcttcctcaa gttctgctcc tacttcccct acaacgcccg gctctgcatc
      841 aacggaaatc actgggctca gcgccaggcc ggcaaggccg ggctcgggtt tgtcccgatg
      901 gacaacgcgt tcgccgcggt cgatgaccca gctacgttgc aggccatctg cgaccggctc
      961 gggccggagc agatcgatgc gctgctgcgc aagtggctcg cgatccttcc gcacccgttc
     1021 agccccgccg accgggccgc cgggtaccgg tacgacatct ccatcctgca ggccgagttc
     1081 tcgctgaccc agatgctcga ccggcccgtc acgggacgga tcttcttcga gcaggtcatc
     1141 cgcgacaacc tcgacgtcgg ccgtcccgac cacgtgtcac tgatcttcga ccggcgcatc
     1201 gtggccaagg gcaaacaccc gacaccgggg cggttccgca cccgagtgat cacgacgggg
     1261 gtcaccccga gtctgcacgt ggactacaag cacacgacca tcaagcagta ccacaaggaa
     1321 ggcagggcac ttcggaccga gaccaccatc aacaacaccc gcgacttcgg aatcgggaag
     1381 cggttgacca acctgcctgc gctacgcgag gtcggcttca ccgccaacag acgcctgctg
     1441 cgcgtcgaac gactcgacca cgacccgacc accggaaccg ccgccctgac cgctgtcacc
     1501 gaaccggtca ccacccctac cggcacccgc gtgcccgggt tacgcctcgg agcgcaacgc
     1561 tcccacgccc tgctatccgc gctgctgatc ttccgactgc aaccagacgg cttcaccaac
     1621 cgcgacctac gggccctcac cgccgagctg cgcggcctgc cacccgagca cgtcaccacc
     1681 ggccagatga cctacgacct gcgtcgcctg cgcgtgcacg gcctgatcga acgcatcccc
     1741 cacacccacc gctaccacct caccgagacc ggactgcgca ccgcgacgtt cctcacccgc
     1801 gtccacgacc gctacctacc taccggcctg gcccacctca acggccccgg cgacaacaga
     1861 ctccgccgcg cccagaccgc ctaccagcac gccctcgagg acctcgcccg acaagcaggc
     1921 ctccccgcgt gacgttgacc ccaaccacca gaacacgact cagaattccg gctccgccgg
     1981 ccctagccct ctagagggag tcagccgaca aggtcgcggc gcagccgctc gacgtgcccg
     2041 gtcgccttca cgt
//
LOCUS       DASRKH010000173         2725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_33945, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000173 DASRKH010000000
VERSION     DASRKH010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33945"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2725)
                     /gene="rpoB"
                     /locus_tag="VFC06_02890"
     CDS             complement(<1..>2725)
                     /gene="rpoB"
                     /locus_tag="VFC06_02890"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015776389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HZL80878.1"
                     /translation="EFAEYPVVLETLEKDTVKTQEEALVDIYKKVRPGEPPTVEAGKN
                     LLENYYFNDKRYDLAKVGRYKINKKLGLEAELSASVLRLDDIVAAIKYICALHKGATT
                     MPGRRNGEDVELRVETDDIDHFGNRRIRAVGELIQNQVRTGLSRMERVVRERMTTQDV
                     EAITPTTLINIRPVVASIREFFGTSQLSQFMDQNNPLAGLTHKRRLSALGPGGLSRDR
                     AGMEVRDVHPSHYGRMCPIETPEGPNIGLIGSLATYARINPFGFVETPYRKVVDGVVT
                     DDVVFLTADDEDRHIIAQASALVDEDGNFTEDHVLVRSSGGEPALVPIDEVDYMDVSA
                     RQMVSVATALIPFLEHDDANRALMGANMQRQAVPLIRSEAPLVGTGMELRAAVDAGDV
                     VVASKAGVVTEVSADAVHVAADDGSTQVYRIQKFRRSNQGNSYNQRVLVDEGQRVEFG
                     GVLADGPATDKGELSLGRNLLVAFMSWEGYNYEDAIILSQRLVADDVLTSIHIEEHEI
                     DARETKLGPEEITRDIPNVADDVLANLDERGIIRIGAEVTSGDILVGKVTPKGETELT
                     SEERLLRAIFGEKAREVRDTSLKVPHGESGIVIGVREFNADDDDELPPGVNQMVRVYI
                     AQRRKITDGDKLAGRHGNKGVISRILPVEDMPFMPDGTPVDIILNPLGVPGRMNMGQV
                     LELHLGWIAAQGWDASEAAAKGLEWVKNLPDGAIQGAPGSRVGTPVFDGVHTDELSGL
                     LECTLPNRDGEVMVNAQGKAQLVDGRSGEPFPDPISVGYMYILKLHHLVEDKIHARST
                     GPYSMITQQPLGGKAQFGGQRFGEMEVWALEAYGAAYALQELLTIKSDDVAGRVKVYE
                     AIVKGENVPEPGVPESFKVLIKEMRSLCLNVEALDAEGNAIELRDNDDEIYR"
BASE COUNT          388 a          919 c          924 g          494 t
ORIGIN      
        1 cggtagatct cgtcgtcgtt gtcccggagt tcgatggcgt tgccttcggc gtcgagggcc
       61 tcgacgttca ggcacagcga ccgcatctcc ttgatgagga ccttgaacga ctcgggaacc
      121 ccgggctccg ggacgttctc gcccttgacg atggcctcgt agaccttcac gcgtcccgcc
      181 acgtcgtcgg acttgatggt cagcagctcc tggagcgcgt aggcggcgcc gtacgcctcc
      241 agtgcccaga cctccatctc gccgaaccgc tgcccgccga actgcgcctt gccgcccagc
      301 ggctgctggg tgatcatcga gtacgggccg gtggaccggg cgtggatctt gtcctcgacc
      361 aggtggtgca gcttcaggat gtacatgtat ccgaccgaga tcgggtccgg gaacggttcg
      421 ccggatcggc cgtcgacgag ctgggccttg ccctgcgcgt tgaccatcac ctcgccgtca
      481 cggttcggca gggtgcactc cagcaacccg gagagctcgt cggtgtggac accgtcgaac
      541 acgggcgtgc cgacccgcga gcccggggcg ccctggatgg cgccgtccgg gaggttcttc
      601 acccattcga ggcccttggc ggccgcttcg gaagcgtccc agccctgcgc ggcgatccac
      661 ccgaggtgga gttcgagcac ctggcccatg ttcatgcggc ccggcacgcc cagcgggttg
      721 aggatgatgt caaccggcgt gccatccggc atgaacggca tgtcctccac cggcaggatg
      781 cgcgagatga cgcccttgtt gccgtggcgg ccggccagct tgtcgccgtc ggtgatcttg
      841 cgccgctgcg cgatgtacac ccgcaccatc tggttgacac ccgggggcag ttcgtcgtcg
      901 tcgtccgcgt tgaactcgcg gacgccgatc acgatgcccg actcgccgtg cggcaccttc
      961 agcgacgtgt cgcgcacctc gcgcgccttc tcgccgaaga tcgcacggag cagccgctcc
     1021 tcggacgtca gctcggtctc gcccttcggg gtgaccttgc cgaccaggat gtcgccggac
     1081 gtgacctcgg cgccgatgcg gatgatgccg cgctcgtcca ggttcgccag cacgtcgtcc
     1141 gcgacgttcg ggatgtcgcg cgtgatctcc tcggggccaa gcttggtctc gcgggcgtcg
     1201 atctcgtgtt cctcgatgtg gatcgaggtg agcacgtcat cggccacgag ccgctgggag
     1261 aggatgatcg catcctcgta gttgtagccc tcccaggaca tgaacgcgac cagcaggttg
     1321 cgccccagcg agagctcgcc cttgtccgtc gccggcccgt cagccagcac cccgccgaac
     1381 tcgacccgct ggccctcgtc caccagcacg cgctggttgt acgagttgcc ctggttggag
     1441 cgccgaaact tctggatgcg gtacacctgc gtggagccgt cgtcggccgc cacgtggacg
     1501 gcgtccgcgg acacctccgt gacgacgccc gccttcgagg cgacgacgac gtcaccggcg
     1561 tcgacggcgg cgcgcagctc catcccggtg cccacgaggg gcgcctcgga acggatcagc
     1621 ggcacggcct gacgctgcat gttcgctccc atgagggcgc ggttcgcgtc gtcgtgctcc
     1681 aggaacggga tcagggcggt cgcgaccgac accatctggc gcgccgagac gtccatgtag
     1741 tcgacctcgt cgatcgggac gagcgccggc tcgccacccg aactccgcac caggacgtgg
     1801 tcctcggtga agttgccgtc ctcgtcaacc agtgcggagg cctgggcgat gatgtggcgg
     1861 tcctcgtcgt cggcggtcag gaacacgacg tcgtcggtca cgaccccgtc gaccaccttg
     1921 cggtacggcg tctccacgaa cccgaacggg ttgatgcggg cgtaggtggc caaggagccg
     1981 atcaggccga tgttcgggcc ttcaggggtt tcgatgggac acatgcggcc gtagtgcgac
     2041 gggtggacgt cgcggacctc catgccggcg cggtcgcgcg acaggccacc ggggccgagg
     2101 gccgagaggc ggcgcttgtg ggtcaggccc gcgagcgggt tgttctggtc catgaactgc
     2161 gagagctggg acgtcccgaa gaactcgcga atcgaggcga ccacgggccg gatgttgatg
     2221 agcgtcgtgg gcgtgatcgc ctcgacgtcc tgcgtggtca tgcgttcgcg aaccacccgc
     2281 tccatccggg acagtccggt gcggacctgg ttctggatga gctcgccgac ggcgcggatc
     2341 cggcggttgc cgaagtggtc gatgtcgtcg gtctcgacgc gcagttccac gtcctcgccg
     2401 ttgcggcgcc cgggcatggt ggtcgcaccc ttgtgcagcg cacagatgta cttgatcgcc
     2461 gccacgatat cgtcgagccg cagcacggac gcggatagtt ccgcctcgag gccgagcttc
     2521 ttgttgatct tgtagcggcc gaccttggcc aggtcgtagc gcttgtcgtt gaagtagtag
     2581 ttctccagca ggttcttgcc ggcctcgacc gtgggcggct cgcccgggcg caccttcttg
     2641 tagatgtcga ccagggcctc ctcctgggtc ttgaccgtgt ccttctcgag ggtctcgagc
     2701 accaccggat actcggcgaa ctcgg
//
LOCUS       DASRKH010000174         3111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_10551, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000174 DASRKH010000000
VERSION     DASRKH010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10551"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VFC06_02895"
     CDS             <1..248
                     /locus_tag="VFC06_02895"
                     /inference="COORDINATES: protein motif:HMM:NF025200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4192 family protein"
                     /protein_id="HZL80879.1"
                     /translation="APQAGALDALERLVAHVTWLCPRDMAPAMTVLGLARWWHGDESG
                     AAHAVALALGDDPRYRLAELIKCAIDAHMPPGWLTAA"
     gene            392..1675
                     /locus_tag="VFC06_02900"
     CDS             392..1675
                     /locus_tag="VFC06_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZL80880.1"
                     /translation="MPSDRSGVGSAKRAGSSRATGESGHNSAPTPKKEKTTSATAPTE
                     DIAPKVAPRATKAAAAVRSKVNKAKAAAAAAPSPDEPEAVPEEAEFKPDAKPVIEGPE
                     EIVGFVISGEEDDAPVQQVMTAGATADPVKDYLKQIGKVALLNAEQEVDLAKRIEAGL
                     FAEEKLGAGEKITPKNRRELEWIANDGRHAKNHLLEANLRLVVSLAKRYTGRGMLFLD
                     LIQEGNLGLIRAVEKFDYTKGYKFSTYATWWIRQAITRAMADQARTIRIPVHMVEVIN
                     KLARVQRQMLQDLGREPTPEELARELDMTPEKVVEVQKYGREPISLHTPLGEDGDSEF
                     GDLIEDSEAVVPADAVGFTLLQEELSKVMDTLSDREAGVVGMRFGLTDGQPKTLDEIG
                     RVFGVTRERIRQIESKTMSKLRHPSRSQVLRDYLD"
     gene            complement(1722..1916)
                     /locus_tag="VFC06_02905"
     CDS             complement(1722..1916)
                     /locus_tag="VFC06_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80881.1"
                     /translation="MNINSYSDIYLQTQSAGLAERNEQRRRQLEAIGADVPAPRRTVR
                     MPQWFAGSLKFIGYHRPATS"
     gene            complement(2053..2856)
                     /locus_tag="VFC06_02910"
     CDS             complement(2053..2856)
                     /locus_tag="VFC06_02910"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HZL80882.1"
                     /translation="MEDAVYWQAMALDAATSRAMDAQGGERVALITTALAQATSLYKD
                     GIDEQPEMCRELLVSACRAQATLTRLGEAPDPRLEARLHEIQKIVESDAVGEPYALVG
                     DAFVRETASETGALDAWRAAMSLIETEPVQVAVPRRLEHEARYRLAVALADAGEKAEA
                     VALLRRVVHEAPPHGIALVARWAREALKRLGADSTPTSGPLEGLTPRELEVLALVAEG
                     LTNREIGARLFMSPKTASVHVSAILAKLGASNRTEAATLYTTRLARDPD"
BASE COUNT          563 a          977 c         1049 g          522 t
ORIGIN      
        1 tggctccaca agcaggggca ttggacgccc tggaacgact ggtcgcccac gtgacgtggt
       61 tgtgtcctcg cgacatggcc cccgccatga cggtcttggg cttggcgcga tggtggcacg
      121 gcgacgagtc gggagcggct catgccgtgg cgttggcact gggcgatgac ccccgttatc
      181 gcctcgcgga actgatcaag tgtgcgatcg atgcccacat gccgccggga tggctcaccg
      241 cggcgtgagt gttacgcggc gtatgcgtag ttttgcgtcg cggggaagcg tggagcggaa
      301 agtcgacgtt gacagaggcg ttagacttta atggatttgc aggcggtgat gagagccgcg
      361 gcagtccccc aatgtgctcg agaggactct tgtgccgtct gatcgttccg gagtaggaag
      421 cgcaaagcga gcgggttcgt cccgcgccac cggagagtca ggtcacaatt cggctcccac
      481 cccaaagaag gagaagacca cgtccgctac tgcccccacc gaggacatcg ctcccaaggt
      541 tgcgccccgc gccaccaagg ccgcggccgc cgtgcgctcg aaagtcaaca aagctaaggc
      601 ggcagccgcg gctgcgccgt cccctgatga gccggaggcg gttcccgaag aggccgagtt
      661 taagcccgac gccaagcccg tgatcgaagg gccggaagaa attgtcggct tcgtcatctc
      721 gggcgaggaa gacgacgcgc ccgttcagca ggtgatgacg gccggagcca cggccgaccc
      781 cgtgaaggac tacctcaagc agatcggcaa ggtcgcgctg ctgaacgcag agcaagaggt
      841 cgatctcgcc aagcggatcg aggccggact gttcgccgaa gagaagctcg gcgcggggga
      901 gaagatcacc ccgaagaacc gtcgcgaact cgagtggatc gcgaacgatg gccgccacgc
      961 gaagaatcac ctgcttgagg ccaacctgcg cctcgtggtg tcactcgcca agcgctacac
     1021 gggtcgcggc atgctgttcc tcgacctcat ccaggagggc aaccttggcc tgatccgtgc
     1081 ggtcgagaag ttcgactaca ccaagggcta caagttctcc acctacgcga cgtggtggat
     1141 ccgccaggcg atcacccgcg ccatggccga ccaggcccgc accatccgca tcccggtgca
     1201 catggtcgag gtcatcaaca agcttgcccg cgtgcagcgt caaatgctcc aggacctggg
     1261 ccgcgagccc acccctgagg aactggcccg cgagctcgac atgacgcccg agaaggtggt
     1321 cgaggtccag aagtatggcc gcgaacccat ctcgctgcac accccgcttg gcgaggacgg
     1381 cgatagcgaa ttcggcgacc tcatcgagga ctccgaggcc gtcgtacccg cggacgccgt
     1441 gggattcacg ctgctgcagg aagagctcag caaggtcatg gacacgttgt ccgaccgcga
     1501 ggccggagtg gtgggaatgc gcttcggact caccgacggt cagcccaaga ctttggatga
     1561 gattggccgt gtgttcggag tcacccgcga gcgcatccgc cagatcgagt ccaagacgat
     1621 gtcgaagctg cgccaccctt cacgctctca agtgctgcgc gactacctgg actagacgag
     1681 acctcgacga tcggtcgagg tctcgacttc gcgcactccg ctcaggatgt ggcggggcgg
     1741 tggtagccga tgaacttcag cgagcccgcg aaccactggg gcatgcgcac ggtgcgtcgc
     1801 ggagccggga catcggcccc aatcgcctca agttgacggc gacgctgctc gttgcgctcg
     1861 gcgaggcccg cggactgcgt ctgaaggtag atgtcgctgt agctgttgat gttcatgatc
     1921 gctccccttg ttgtgaccga cccgaggccg atgtgctgac aagtgagaat ctgctcccta
     1981 aggcgggtgc agaacatcgg gagaatgccc tatcttttgg gcgggcggtg ccttagttcc
     2041 gcgggcgggt ccttagtccg ggtcgcgtgc cagccgtgtc gtgtaaagcg tggcggcctc
     2101 ggtgcggttc gatgcgccga gtttggcgag tatcgcggac acatggacgc ttgcggtctt
     2161 gggggacata aagagacgcg cgccgatctc gcggttggtc aggccctccg cgaccagcgc
     2221 gaggacctcg agttcgcgcg gcgtgagtcc ctcgagcggg cctgatgttg gggtgctatc
     2281 ggcgccgagc cgctttagtg cctcccgagc ccagcgtgcg acgagggcga tgccgtgggg
     2341 cggggcctca tggaccactc gtcgaaggag ggcgaccgcc tcggctttct cgccagcatc
     2401 ggcaagcgcc acggcgaggc ggtaccgcgc ctcgtgctcc agccgtctgg gcaccgccac
     2461 ctgcaccggc tcagtctcaa tgagcgacat ggccgcgcgc caggcgtcga gcgcgccagt
     2521 ttcgctcgcc gtctcccgaa cgaaggcgtc gccgacaagc gcgtagggct cgcccacggc
     2581 atcgctctcg acgattttct ggatctcatg caagcgagcc tccaaccgcg ggtctggcgc
     2641 ctcgccgagc cgggttagcg ttgcctgagc gcggcaagcg gacaccaaca actcgcggca
     2701 catctccggc tgctcgtcga ttccatcctt gtagagcgac gtcgcctgcg cgagtgcggt
     2761 cgtgatcagc gcgacgcgct cgccgccttg agcgtccatg gcacgagacg tcgcggcatc
     2821 aagggccata gcctgccagt atacggcgtc ctccatgaga cccgcaaggt cgcgacgtgc
     2881 ctcagtttct ttgagcgcga gggatgggtt ggtgcctcag catctttgag cgcgtagccc
     2941 ctcgtgcggg ggcaggcttg cctgatggta gaaggcaaga ttccgatggc cgcgaggcga
     3001 caagtcacga acaagctccg gaccgtttac gcgagagcgt cgaagcggga caaggccagg
     3061 atcctcgatg aggtgatgtc gacgacgggg atgggccggt ccacggcgcg g
//
LOCUS       DASRKH010000175         2694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_14129, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000175 DASRKH010000000
VERSION     DASRKH010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14129"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VFC06_02915"
     CDS             <1..479
                     /locus_tag="VFC06_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HZL80883.1"
                     /translation="DTDDDLKARVGWIAYFMSGVRHLHGRRLRALVRLDDRPPTAVRL
                     RSLLVGNCGRLPGGLTLLPDAVLDDGWLDVAAIDTRGGVAGWAQLFGEVVLQGVGVRA
                     ELPARIGRIDHARARRVLVRVSTPEHVQVDGDVIGRAQALSARIQAGGLVVRVPRP"
     gene            complement(548..1522)
                     /gene="pheA"
                     /locus_tag="VFC06_02920"
     CDS             complement(548..1522)
                     /gene="pheA"
                     /locus_tag="VFC06_02920"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HZL80884.1"
                     /translation="MSVPRYAYLGPAGTFTEAALRQVVGPDEAEYVPQVDVVSAIEAV
                     RSGDADFAVVAIESTVEGGVTATLDSLAVGEPLVLLREVLVPVDFTLAAAPGTALADV
                     RRVAAHPHAWVQCRRWLTAHLPGAVHVPATSNTAPAALIADSLADTSEGDPAAGLQLG
                     FDAALVPPAAVGSYGLAVLAEHVADNPSLVTRFVVVGRPGEVPPATGADKTTLDVHLP
                     SNDAGALLTMLEQFAVRGVNLSRIESRPVGDSLGRYSFSIDAEGHIAEERMGEALMGL
                     HRVCPHVRFLGSYPRADDVRAVVHIGTSDRDFRDAREWLGQLRDGRAH"
     gene            1593..>2694
                     /locus_tag="VFC06_02925"
     CDS             1593..>2694
                     /locus_tag="VFC06_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80885.1"
                     /translation="MPMPPRATRSARATRARRFSTVGVLAAVGGLLALLTLPASTAVA
                     TTRTAATGTAASAGTGSPVVLVGVTGLRWDDLGSLTTPALWSLSREAAVGTTVVRSVR
                     PSSCPADGWLAVSAGTRAADLPAEDGTCRRLHDPVQGSAVPGWADYLESAATELFSAH
                     VGLLGDTLAAGGATVTGIGPGAAIALADATGTAVGEQVRLPSDAAKLRQLVRESLTTS
                     DLVVVDAGSVRDPGYTTQDRLPADTADAGAPVVVGPDGEDLTSDLDAAVAIMEPTRSE
                     QAQAIDARVRAVLTAIDTSKTDPTVLLVSLADSGRSPQLQLAIASGPAAEGDGGTYAG
                     ALLGSRSTRQPGYLQATDVTPTLLSALGLRAEA"
BASE COUNT          340 a         1024 c          997 g          333 t
ORIGIN      
        1 cggacaccga tgacgacctc aaggcgcgcg tcgggtggat cgcctacttc atgtcggggg
       61 tgcggcacct gcacggtcgc cggttgcgag ccctcgtgcg gctcgacgac cggccgccga
      121 ccgcggtgcg gctgcgcagc ctgctcgtcg gcaactgcgg acgtcttccc ggcgggctca
      181 cgctcctgcc cgatgcggtg ctcgacgacg gctggctcga cgtcgcagcg atcgacaccc
      241 gcgggggcgt cgccgggtgg gcccagctgt tcggcgaggt cgtgctccag ggcgtcggcg
      301 tgcgcgccga gctgcccgcg aggatcggcc gcatcgacca tgcccgggcc cgccgggtgc
      361 tcgtccgcgt ctccacaccc gagcacgtgc aggtcgacgg cgacgtgatc ggcagggctc
      421 aggccctgtc ggcccgcatc caggcgggtg ggctcgtcgt gcgcgtgccg cggccctgag
      481 cactgcccga cccctgagca ctggccgacc ggaggtcagc gcgcgccgag atcagtgcgc
      541 gctgaggtca gtgcgcgcgg ccgtcccgca gctgcccgag ccactcgcgc gcgtcgcgga
      601 agtcccggtc ggacgtgccg atgtgcacga ccgcgcgcac gtcgtccgcg cgcgggtagg
      661 agcccaggaa ccgcacgtgc gggcacaccc ggtgcagccc catgagcgcc tcacccatgc
      721 gctcctcggc gatgtggccc tcggcgtcga tcgagaacga gtaccggccg agcgagtcac
      781 cgacgggccg cgactcgatg cgtgacaggt tcacgccccg gaccgcgaac tgctcgagca
      841 tcgtgagcag cgcaccggca tcgttgctcg ggaggtgcac atcgagcgtc gtcttgtcgg
      901 ctcccgtcgc cggcgggacc tcgcccgggc gcccgaccac gacgaagcgc gtgaccagcg
      961 acgggttgtc cgcgacgtgc tcggcgagca ccgcgagacc gtaggacccc acggccgcgg
     1021 gcggcaccag cgccgcgtcg aacccgagct ggagcccggc cgccgggtca ccctcggacg
     1081 tgtccgcgag cgagtcggcg atgagcgccg cgggagcggt gttggacgtc gcgggcacgt
     1141 gcaccgcgcc gggcaggtgc gcggtgagcc agcgtcggca ctgcacccag gcgtgcgggt
     1201 gcgcagccac ccgtcgcacg tccgcgaggg cggtcccggg cgcggccgcg agcgtgaagt
     1261 cgaccgggac gagcacctcg cgcagcagca cgagcggctc gccgactgcg agcgagtcca
     1321 gcgtcgccgt gaccccgccc tcgaccgtgc tttcgatcgc gacgaccgcg aagtccgcat
     1381 cgcccgagcg cacggcctcg atcgcggaca cgacgtccac ctgcgggacg tactcggcct
     1441 cgtccggacc gacgacctgg cggagggccg cctccgtgaa cgtcccggca gggccgaggt
     1501 aggcatagcg cgggacggac atctgccctc cggtggtgcg gggatgcggc ggcgaaagcg
     1561 gtgcgagcct agtgcgaccg taggcttgag cggtgccgat gcccccccgc gcgacccggt
     1621 ccgcccgcgc gacccgtgcg cggcggttct cgacggtcgg cgtgctcgcg gccgtcggag
     1681 gactcctcgc gctgctcacc ctccctgcgt ccaccgcggt cgcgacgacc cgcaccgcgg
     1741 cgaccggcac cgcggcgtcg gcggggaccg ggtcaccggt cgtgctcgtc ggcgtgaccg
     1801 gtctgcgctg ggacgacctc ggctcgctca cgacccccgc gctgtggagc ctgtcgcgcg
     1861 aggccgccgt gggcacgacg gtcgtgcgca gcgtgcggcc gtcgtcgtgc cccgcggacg
     1921 gatggctcgc ggtgtctgcc ggcacgcgcg cggcggacct gccggccgag gacggcacgt
     1981 gccgacgtct gcacgacccg gtccagggct cggccgtgcc gggctgggcc gactacctgg
     2041 agtccgccgc gacagaattg ttctccgcac acgtggggct gctcggtgac actctcgccg
     2101 ccgggggcgc gacggtcacc ggcatcggcc cgggtgcggc gatcgccctc gctgacgcga
     2161 ccggcacggc cgtcggcgag caggtgcggc tgccgtccga cgcggccaag ctcaggcagc
     2221 tcgtgcgcga gtcgctcacg acctcggacc tcgtcgtcgt cgacgccggg tcggtgcgcg
     2281 accccggcta cacgacgcag gaccggttac cggccgacac ggcggacgcc ggtgcgcccg
     2341 tcgtggtcgg gcccgacggc gaggacctca cgtccgacct cgacgccgcc gtcgcgatca
     2401 tggaaccgac acgcagcgag caggcgcagg ccatcgacgc ccgcgtgcgc gccgtgctca
     2461 cggcgatcga cacgtcgaag accgacccca ccgtgctcct cgtctcgctc gccgactccg
     2521 ggcggagccc ccagctgcag ctcgccatcg cgagcggacc ggccgccgag ggcgacggcg
     2581 gcacgtacgc cggggcgctg ctcggttcgc gatcgacgcg ccagcccggc tacctgcagg
     2641 ccaccgacgt caccccgacc ctgctgagcg ccctgggact gcgcgccgag gcac
//
LOCUS       DASRKH010000176         2698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_9221, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000176 DASRKH010000000
VERSION     DASRKH010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9221"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VFC06_02930"
     CDS             <1..395
                     /locus_tag="VFC06_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HZL80886.1"
                     /translation="ISRAVAAIAEAHHDDFGLAWPIQVAPAHVHIIATGKEAEVFDAA
                     RELAVALEADGVEVLYDDRPRVSAGVKFADSELLGVPFVVVVGRGLAEGTVEVRERRA
                     GNRRAVPVADAVAVVARTVRDALAAAAI"
     gene            complement(399..1277)
                     /locus_tag="VFC06_02935"
     CDS             complement(399..1277)
                     /locus_tag="VFC06_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80887.1"
                     /translation="MTTRLLGLLLALVALTGCGVRLDSPDPVPSSPDAAEVVRQREAL
                     RARQFAAVEPEGEHADLVAAVATHASAQLDALGGVWVAWPAGDGPTPTADATADVVIG
                     PGAAGLLDSLTATTPDVAAAALAGGDPEIATLYAAIATARTVDADRLAVALGTPGAVT
                     PLPGSLDTPDPAVARALDAAAYRLETLAARERAADAAAEAERFVARAGELRSLAEGIV
                     AANGWLGTAADPRAPYYPVTEDDAATLHRDLTVLLVAAVGDSDDRAGMLDAALSCALE
                     ASRRGQELGALPGLAS"
     gene            1365..1817
                     /gene="rimP"
                     /locus_tag="VFC06_02940"
     CDS             1365..1817
                     /gene="rimP"
                     /locus_tag="VFC06_02940"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HZL80888.1"
                     /translation="MARELQDTLTSHLGPVVAGAGLLLEGVKVASAGRRKLVRAVVDL
                     PDGPGGVSSDALIEVSRLISQALDEADLVPGSYTLEVTTPGVDRPLTEPRHFRRAVGR
                     LVEVTTPAGTRAGRLTEVTPDTIVLGDAVIALADISAALTQVELTRED"
     gene            1819..>2698
                     /gene="nusA"
                     /locus_tag="VFC06_02945"
     CDS             1819..>2698
                     /gene="nusA"
                     /locus_tag="VFC06_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HZL80889.1"
                     /translation="MDIDMNALRMVETDRGIPMPVLLSAIEEALLVAYHRQPGAIPEA
                     RVEVDRKTGAVLVMATEFDEEGNAIGEFDDTPSGFGRIAAATARSVIMQRLRDAEDTQ
                     IMGVYRGKAGDTIAGVIQADRSTRNVMVNVGDLEAVLPVHEQVAGEKYNHGDRIRCYV
                     VDVSRGPKGPSITLSRSHPNLVRRLFALEVPEVADGTVEIAAVAREAGHRTKMAVMSP
                     IPGFNAKGACIGPMGQRVRAVMSELQGEKIDIVTWSDDPAEFVAAALSPARVTSVEIV
                     SYDTRTARVVVPDYQLS"
BASE COUNT          361 a          987 c         1006 g          344 t
ORIGIN      
        1 gcatctcgcg cgcggttgcc gcgatcgccg aggcgcacca tgacgacttc ggcctcgcct
       61 ggcccatcca ggtggcgccc gcccatgtcc acatcatcgc gaccggcaag gaggccgagg
      121 tcttcgacgc cgcgcgcgaa ctggccgtcg cgctcgaggc cgacggtgtc gaggtgctgt
      181 acgacgaccg gccgcgcgtg agcgcgggcg tcaagttcgc ggactcggag ctgctggggg
      241 tgccgttcgt cgtcgtggtc ggccggggac tcgccgaggg cacggtcgag gtgcgggagc
      301 ggcgcgcagg gaaccgccgc gcggtgccgg tcgcggacgc cgtcgccgtc gtggcacgga
      361 ccgtgcggga cgcgctcgcc gccgcggcga tctgaccgtc aggacgccag ccccggcagc
      421 gcgccgagct cctggccgcg ccgggacgcc tcgagcgcgc agctgagcgc ggcgtcgagc
      481 atgcccgcgc ggtcgtccga gtcgccgacg gcggcaacga ggagcaccgt caggtcgcgg
      541 tgcagggtgg cggcgtcgtc ctctgtcact gggtagtacg gtgcccgcgg gtccgctgcc
      601 gtgcccagcc acccgttcgc cgcgacgatc ccctcggcaa gcgaccgtaa ctccccggcc
      661 cgcgcgacga accgctccgc ctcggccgcc gcgtccgctg cccgctcccg cgcggcgagg
      721 gtctccagcc ggtaagccgc ggcgtccagg gcccgggcga cggccggatc gggggtgtcc
      781 agcgacccgg ggagcggggt gaccgccccg ggcgtgccga gggcgacggc gaggcggtcc
      841 gcgtccaccg tcctcgccgt ggcgatggcg gcgtagaggg ttgcgatctc cgggtcgccg
      901 ccagccaggg cggcggcggc gacgtccggc gtggtggcgg tgagcgaatc cagcaggccc
      961 gcggcccccg ggccgatgac gacgtcggcg gtggcgtccg ccgtcggggt ggggccgtcc
     1021 ccggcgggcc aggccaccca cacgcccccc agtgcgtcca gctgggcact ggcgtgggtg
     1081 gcgaccgcgg cgacgaggtc cgcgtgctcg ccctccggtt caacggccgc gaactgtcgc
     1141 gcgcgcagcg cctcgcgctg gcgcaccacc tccgcggcgt ccggcgagga gggcacggga
     1201 tcgggcgagt cgaggcgcac gccgcacccg gtgagcgcca cgagggcgag gaggaggccc
     1261 agcagtctcg tcgtcacccc ccgatcctgc caccgaccgg cccggtgcac ccgcatcggc
     1321 ccacaggcta ccatgggggt cgagaacttc agaaggaggt cgagatggca cgcgagctgc
     1381 aggacacgct cacttcacac ctcggtccgg tcgtggccgg cgcgggactc ctcctcgagg
     1441 gcgtgaaggt ggcctcggcc ggacggcgca agctggtacg cgccgtcgtc gacctcccgg
     1501 acggccccgg cggcgtctcg tcggacgcgc tgatcgaggt ctcccgcctg atctcccagg
     1561 ccctcgacga ggccgacctc gtcccgggca gctacacgct cgaggtcacc accccgggtg
     1621 tggaccggcc gctgaccgaa ccacgccact tccggcgggc cgtggggcgg ctcgtcgagg
     1681 tcaccacgcc ggcgggcacg cgggccggcc gcctgaccga ggtgacgccg gacaccatcg
     1741 tgcttgggga tgcggtgatc gccctcgctg acatcagcgc ggctctcacc caggtggaac
     1801 tcacgaggga ggactgacat ggacatcgac atgaacgccc tgcggatggt cgagaccgac
     1861 cgcggcatcc cgatgcccgt gctcctgtcc gccatcgagg aggcgctcct ggtggcgtac
     1921 caccgccagc cgggtgcgat cccggaggcg cgggtggagg tggaccgcaa gaccggcgcg
     1981 gtcctggtga tggccaccga gttcgacgag gagggcaacg cgatcggcga gttcgacgac
     2041 accccctccg gtttcggccg gatcgccgcc gccaccgccc gctccgtcat catgcagcgg
     2101 ctgcgggacg ccgaggacac ccagatcatg ggcgtctacc gtgggaaggc gggcgacacg
     2161 atcgccgggg tgatccaggc cgaccggtcc acccgcaatg tcatggtcaa tgtgggcgac
     2221 ctcgaggcgg tcctgccggt gcacgagcag gtcgcgggcg agaagtacaa ccatggcgac
     2281 cggatccgct gctacgtggt ggacgtctcc cgcggcccca agggcccctc gatcaccctg
     2341 tcgcgctccc acccgaacct cgtgcgccgc ctcttcgcgc tggaggtccc cgaggtggcc
     2401 gacgggacgg tggagatcgc cgcggtcgcg cgtgaggccg ggcaccgcac caagatggcg
     2461 gtcatgtccc ccatcccggg cttcaacgcg aagggcgcgt gcatcggccc gatgggccag
     2521 cgtgtgcgcg ccgtcatgag cgaactgcag ggcgagaaga tcgacatcgt cacctggtcg
     2581 gacgaccccg cggagttcgt ggcggcggcg ctctcgccgg cgcgggtcac ctcggtggag
     2641 atcgtcagct acgacacgcg gactgcccgc gtcgtggtcc ccgactacca gctctccc
//
LOCUS       DASRKH010000177         3838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_4284, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000177 DASRKH010000000
VERSION     DASRKH010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4284"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /locus_tag="VFC06_02950"
     CDS             complement(<1..967)
                     /locus_tag="VFC06_02950"
                     /inference="COORDINATES: protein motif:HMM:NF024586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HZL80890.1"
                     /translation="MTSADISRSRWPQTVARRFLDAARKLRPPVRSRQFWLVQAGVLF
                     VAFLDLVVLDHMGFQLPLRIPNAMITALLLIPVVYAALNFGVRGAVRTALWATTLLIL
                     DWISLHHGTHTDMWIEVGNLATLNAVAVVVGQRVEREGRARLRAKGALRGSEIAEARY
                     RALFDEQHAPVLVTDSAGVVTEANAAATSLLGTGAPGRLLSDLVDAPVTAILDGTVAR
                     LSVGERVFVAKARTLEVGPGERLVQIVLVDTTEEERRLREQRAYAAHLVTVQEDERRK
                     LAQDLHDDPVQTLTYLVRMLELHNDDPRLPPELAARARRNGELAAG"
     gene            complement(977..1639)
                     /locus_tag="VFC06_02955"
     CDS             complement(977..1639)
                     /locus_tag="VFC06_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZL80891.1"
                     /translation="MVTMETDVIRVVVVDDHPLHRDGTRQILEAYPDLQVVGEATSGE
                     VALALVNQLRPDVVLMDIRLPGMNGIEATRALTRDHPDVRVLMVSAYDEDVYVRSALE
                     AGAAGYLRKTAPGKELVGAVRAVAGGATVLQSGLTTRLLIASRQPEQGPVPLTERELA
                     VLRLLAEGLRNRELAARLGISPRTVERHCASVYAKLGVGSRTEAVVLAISTKLLSVGD
                     DQ"
     gene            1991..2845
                     /locus_tag="VFC06_02960"
     CDS             1991..2845
                     /locus_tag="VFC06_02960"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HZL80892.1"
                     /translation="MFDDDQYLQNAKEFVDAADWDSAPKVRIELGEMFFEPEDVRLEA
                     GKPYVIELVNTGKVKHEFAAPKFFRSVATRKVENPGSEVKVPYFTEIEVLAGESIELF
                     VIPTVPGSFEMVCEIEGHREAGMEGTITVTGSAPTVPVPVIGSVKAAPWLQNGPALIE
                     AAAATWDTTAQTIQIEAGEDGATMFFKPNNIVLKVGTPYVLEFVNVGEIKHEFTADEF
                     FPTIAFRKAEDAFGEYKAPMLKEAEVKAGSQLDLYLIPTVPGTYDIICEIEGHAEAGM
                     FGTITVTE"
     gene            2942..3790
                     /locus_tag="VFC06_02965"
     CDS             2942..3790
                     /locus_tag="VFC06_02965"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZL80893.1"
                     /translation="MQERTTLPEEMPAGPPPGATPDKHPMLSRRNTLAALGVGVAGGA
                     LAMVSYRDDASAATISMASAEKTAEHAWCMVIDLRACDGCKLCTLACQKRHNLRDEQT
                     WINVYEMKDSSGGTFNMPRPCMMCEDPPCMYICPVGANIRTSEGLDLVDTDICVGSRA
                     CMAACPYEARYFNWTDPLPQKRMPMANSPQMPVPQKGTVGKCVFCADRLPYGELPACV
                     SGCPMGALYIGDLVTDTAVNGQGTTVVLSEFLHDNDAVRYKEELGTNPRVYYILGHGQ
                     DLGGGE"
BASE COUNT          731 a         1265 c         1237 g          605 t
ORIGIN      
        1 cccccgcggc cagctcacca ttgcgacggg cccgcgcagc cagctccggt ggcaatctcg
       61 ggtcatcgtt gtgcagctcg agcatgcgca cgaggtaggt cagagtctgc accgggtcgt
      121 catgcaggtc ctgggccagc ttgcgccgct cgtcctcctg cacggtgacc aggtgggcgg
      181 cgtaggcccg ttgttcgcga aggcggcgct cctcctcggt cgtatctacg agcacgatct
      241 gaaccaggcg ttcgccgggg ccgacctcga gcgtgcgagc cttggccacg aacacgcgct
      301 cgcccaccga tagccgcgca actgtgccat ccagaatcgc cgtgaccggg gcgtcgacga
      361 ggtccgacag gaggcgtccg ggcgcaccag tcccgagcag ggatgtggcc gcggcgttgg
      421 cctcggtgac cacccctgct gagtcggtga ccagcaccgg cgcatgctgc tcgtcgaaca
      481 gggcacggta gcgtgcctcg gcaatctcac tgccgcgcag cgcccccttc gcccgcaacc
      541 gcgcccgccc ctcgcgctca acccgctgcc ctacaacgac agcgaccgcg ttgagggtgg
      601 ctaggttgcc gacctcgatc cacatgtccg tgtgcgttcc gtggtggagg gagatccagt
      661 cgaggatcaa gagtgtggtc gcccacagtg cggtgcggac ggctccacga acaccgaagt
      721 tcaatgccgc gtagacgacc gggatcagta gcagcgcggt gatcattgcg ttcgggattc
      781 ggagcggtag ctggaaaccc atgtggtcga gaacgaccaa gtccaggaag gctacgaaca
      841 agactcccgc ctggacgagc cagaactgtc gggatcggac cggcggacgc aacttgcggg
      901 cggcatccag gaaacgtctg gcgacggtct gaggccacct cgagcgcgag atgtcagcgc
      961 tggtcatcgc cgacgctcac tggtcatcgc cgacgctcaa caacttggtc gagatggcaa
     1021 gcacgaccgc ctcggttcgc gaacccaccc ccagcttggc gtagacgctc gcgcagtggc
     1081 gctccacggt ccgcgggctg atccccagcc gtgccgcgag ctcccggttg cgcaggccct
     1141 ccgcgagcag tctcaggact gccagctctc gctcggtcag gggaacgggg ccctgctcgg
     1201 gctggcgtga cgcgatcagc aaccgagtcg tgagaccgga ctggagcacg gtcgccccac
     1261 cggccacggc tcgcaccgcc ccgaccagct ctttgccggg ggcggtcttg cgcaggtacc
     1321 ccgcagcccc tgcctccagc gcactgcgga cgtacacatc ctcgtcatac gcactgacca
     1381 tcagcactcg cacatccggg tggtcccggg tgagcgcgcg ggtggcctcg ataccgttca
     1441 ttcccggtag ccggatgtcc atcagcacca cgtcggggcg caactggttg accagggcga
     1501 gggcaacctc gccggaagtg gcctcaccaa ccacctgcag atcggggtac gcctcgagaa
     1561 tctggcgggt gccatcacga tgcagcgggt ggtcatcgac caccaccacg cggatcacgt
     1621 cggtttccat ggtcaccatg gtctacaggc tacgcgtcat cgcaccgcgg actggcggaa
     1681 aacacccatg cgttatgagt ggaacgacag ccggcactag ggggaaaccc actggcccca
     1741 catgccgcga cccacacata ctggcgtcga cggttgaccg aaacggtgga ccgcgaagag
     1801 tttgtaaggg agcgtcatgg tgcagagcac ggtaattcaa cgggccacgg gaacgcggag
     1861 gagccttcga ctgcttactg tactgggcgt aaccctggca gttctcgcga ccggagcgtg
     1921 tggcacagcc acgactgagg aaccggcagc cggtggagct gccacgccag cggccccagc
     1981 ggccccagcg gtgttcgacg acgaccagta cctgcagaac gccaaggagt tcgtcgacgc
     2041 ggccgattgg gactcggcgc cgaaggtgcg catcgagctc ggtgagatgt tcttcgagcc
     2101 ggaggacgtc cggctcgaag ctggcaagcc ctacgtgatc gagttggtta acaccgggaa
     2161 agtcaagcac gagttcgccg cccccaagtt cttccggtcg gtcgcaaccc gcaaggtcga
     2221 gaaccctggt tccgaagtga aggttccata cttcaccgag atcgaagtct tagccggcga
     2281 gagcatcgag ctcttcgtga ttccgacggt cccgggctcg ttcgagatgg tctgtgagat
     2341 cgaagggcac cgcgaggcag ggatggaagg cacgatcacg gtcaccggat ctgcgccgac
     2401 cgttcccgta cctgttattg gcagcgtcaa ggcagctccg tggctccaga acggcccggc
     2461 cctcatcgaa gcggcggcgg cgacctggga caccacggcg cagaccatcc agatcgaagc
     2521 cggcgaggac ggcgcgacga tgttcttcaa gccgaacaac atcgtcctga aggtcggcac
     2581 cccctacgtg cttgagttcg tgaacgtcgg cgagatcaag cacgagttca ccgctgacga
     2641 gttcttcccc acgattgcgt tccgcaaggc agaagacgcc tttggtgaat acaaggctcc
     2701 gatgctcaaa gaagccgagg tcaaggccgg ctcgcagctg gatctgtacc tgatcccgac
     2761 cgtgccgggg acctacgaca tcatctgcga gatcgaggga cacgcggagg ccgggatgtt
     2821 tgggacgatc acggtcaccg agtagccgcg taggaaccgc acgagaagtg acggagcctg
     2881 cgccgcagtc cggtgactcc atcaaggaag tagatccaga gcgactttga aggagaacac
     2941 catgcaggaa cgtacgacgc tcccggagga aatgccagca ggtcctcctc cgggagccac
     3001 ccccgacaaa cacccgatgc tgagccgccg caacaccctc gccgccttgg gtgtcggcgt
     3061 ggccggcggc gccctggcca tggtcagcta ccgggacgat gcctcggcgg cgaccatatc
     3121 catggcgtcg gccgagaaga cggccgagca tgcctggtgc atggtcatcg acctgcgcgc
     3181 gtgtgacggg tgcaaactgt gcaccctcgc gtgccagaaa cgccacaacc tgcgcgacga
     3241 gcagacctgg atcaatgtct acgagatgaa ggacagttcc ggcgggacgt ttaacatgcc
     3301 gcggccctgc atgatgtgcg aagaccctcc gtgcatgtac atctgcccgg tcggggcgaa
     3361 cattcgcacc tccgaaggtc tggacctggt cgacaccgat atctgtgtcg ggtcccgtgc
     3421 ctgcatggcc gcctgcccgt acgaggcgcg ttacttcaac tggaccgacc cgctgcccca
     3481 gaagcggatg ccgatggcca actcaccaca gatgccggtg ccgcaaaaag gcaccgtggg
     3541 caagtgcgtg ttctgcgccg accggctgcc gtacggggag ttacctgcct gcgtctccgg
     3601 atgcccgatg ggtgcgctgt atatcggcga cctggtcacc gacaccgccg tcaacggcca
     3661 gggcaccacc gtggtgctct cggagttcct gcacgacaac gacgccgtgc ggtacaagga
     3721 ggagctcggg accaacccgc gcgtctacta catcctcggc cacggccagg atcttggagg
     3781 cggggaatga acaacgttgc acgcgaggcg gcactgcgcc cgttcggcaa gcccagcc
//
LOCUS       DASRKH010000178         2847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_54384, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000178 DASRKH010000000
VERSION     DASRKH010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54384"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /locus_tag="VFC06_02970"
     CDS             complement(<1..1175)
                     /locus_tag="VFC06_02970"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HZL80894.1"
                     /translation="MPSITATIDGVERRVQQGTTGLDVYAEHRDVVVMRVAGDLRDLA
                     REIQADDVVEAVLASEPDGLNVLRHSATHVMAQAVQGLRPGAKLGIGPFITDGFYYDF
                     DVEEPFTPEDLKALEKAMQRIVKEGQTFRRRAVTQDEALAELADEPYKCQLLNRAVGV
                     DGAEGVSVEVGAGETTIYDNLNRQGETVWKDLCRGPHVPSTKVLANGWSLMRSAAAYW
                     LGSEKNPQLQRIYGTAWPTKDELLAYKDRLAEAERRDHRRLGADMDLFSFPDEIGSGL
                     AVFHPKGGIIRMEMEEYSRKRHIEEGYEFVYSPHITKAHLFQTSGHLDWYADGMYPPM
                     HMDEERDAAGNITKQGQDYYLKPMNCPMHNLIFRSRGRSYRELPLRMFEFGTVYRNE"
     gene            complement(1302..1826)
                     /locus_tag="VFC06_02975"
     CDS             complement(1302..1826)
                     /locus_tag="VFC06_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80895.1"
                     /translation="MATANKTLDGLALKAGGVAKRLAEARHDAQLAKGEATVLAHDAK
                     EAARLAKKAKSHATKATDAVKKVKLDLKKAEAALKKAQLKAKQKAARDAKKARAKAKV
                     DARKDLATAAPAPAPEPTVERESPEEATTSADHADPVTVAAEEPATPADEKVDDKFDG
                     ALDAVANQAQKVNH"
     gene            2006..2353
                     /locus_tag="VFC06_02980"
     CDS             2006..2353
                     /locus_tag="VFC06_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HZL80896.1"
                     /translation="MTINPVASNPDHYSVLFENERVRVLKYHDEPGTLTLPHDHPDSV
                     MITASSFRRELIQGERTAEVELPAGRAVWLPAQSHAGHNIGETDTDVFFVELKEPSPV
                     DAAAAGRALGPVD"
     gene            2575..>2847
                     /locus_tag="VFC06_02985"
     CDS             2575..>2847
                     /locus_tag="VFC06_02985"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZL80897.1"
                     /translation="MDTSTDGAVVHLFRVEAAVRATLKKNPVDIEAMFDGVVGRYDIT
                     NTIITAGHDNYWRWRATRALGSGPGDTVLDVAAGTGRSTRSLQRAGA"
BASE COUNT          430 a          955 c          888 g          574 t
ORIGIN      
        1 ttctcgttgc ggtacacggt gccaaactca aacatcctca gcggcagttc gcggtacgaa
       61 cgtccgcgcg accggaagat caggttgtgc atcgggcagt tcatgggctt caggtagtag
      121 tcctggccct gcttggtgat gtttcccgcc gcgtcacgtt cctcgtccat gtgcatcggg
      181 gggtacatgc cgtcggcgta ccagtccaag tgccccgagg tctggaagag gtgagccttg
      241 gtgatgtggg gcgagtagac gaactcgtag ccctcctcga tgtggcgctt gcgcgagtac
      301 tcctccatct ccatacggat gattccgccc ttggggtgga acaccgcgag ccccgagccg
      361 atctcgtcgg ggaaggagaa caaatccatg tccgcgccaa gcctgcggtg atcgcggcgt
      421 tcggcctcgg cgagccgatc cttgtacgcg agcagttcgt ccttcgtggg ccatgcggtg
      481 ccgtagatcc gctgcagttg cgggttcttc tcgctgccaa gccagtacgc ggcggcgctg
      541 cgcatgagcg accacccgtt cgcgagcacc ttggtgcttg gcacgtgcgg accgcggcac
      601 aggtccttcc acacggtctc gccttgacga ttgaggttgt cgtagatcgt ggtctcgcct
      661 gcacccacct cgaccgacac gccctcggcg ccatcgactc ctaccgccct gttcagcagc
      721 tggcacttat acggctcgtc ggcgagttcg gccaacgcct cgtcctgggt cacggcgcgg
      781 cgccggaagg tctgaccctc cttgacgatg cgttgcatgg ccttttcgag cgccttcaag
      841 tcctcgggag tgaagggctc ctcgacgtcg aagtcgtagt agaagccgtc ggtgatgaag
      901 ggaccgattc ccagtttggc tccaggtcgc agcccctgca ccgcctgcgc catcacgtgg
      961 gtcgcggagt ggcgcagcac gttgaggccg tcgggctccg acgccagaac tgcctccacc
     1021 acgtcgtccg cctggatctc acgggcgagg tcgcgcaaat cgccggcaac ccgcatcacc
     1081 acgacgtcac ggtgctcggc gtacacgtcg aggcctgtcg tgccctgttg gacccgacgc
     1141 tcgacgccgt caatggtcgc tgtgatgctc ggcacgtgaa gtctccttag agtgagtggc
     1201 gcgcggcgcg tgctccccga cgatgctacc gacccgaggc ctcatgtgga cacacggacg
     1261 gccccggcgg attgcttcac cggggctgtg cgcgcgtact tttagtggtt caccttctgc
     1321 gcttggttcg cgacggcgtc gagggctccg tcaaacttgt cgtcgacctt ctcgtcggct
     1381 ggcgtcgcgg gctcctctgc ggccacggtc accggatccg cgtggtccgc tgacgtggtt
     1441 gcctcttcag gtgattcacg ttcgactgta ggctccggcg cgggcgccgg ggctgccgtc
     1501 gcaagatcct tgcgagcgtc gaccttggcc ttggcgcgcg ccttcttggc gtcgcgcgcc
     1561 gccttctgct ttgctttcag ctgcgccttc ttcagtgccg cttcggcctt cttgaggtcc
     1621 aatttgacct tcttgaccgc atcggtcgcc ttcgtcgcgt ggctctttgc cttcttggcg
     1681 aggcgcgcgg cctccttggc atcgtgagcg agaaccgtcg cttcgccctt cgccaactgc
     1741 gcgtcgtgtc ttgcttccgc aagacgcttc gcgacgcctc cggccttcaa tgccagtcca
     1801 tccagagtct tgttcgcggt ggccatcagt cttcgtgagc tcccttgttg aacgtgtgct
     1861 tagcgctcac tgtagaccgc tcgggatccg cacggccgac ggtggtcccc gatggattcg
     1921 gacatcggga tcgaacacga tggcgtctcc ggaattcgcg cgaactcccc ctgcgcgacg
     1981 ccgcgccaac ctgtaccttc gagtcatgac gatcaaccct gttgcgtcca acccggatca
     2041 ctactcggtg cttttcgaga acgagagggt cagggtgctc aagtatcacg acgagccagg
     2101 aaccctcacg ctgccccatg atcaccccga ttcggtgatg atcacagcga gcagcttccg
     2161 cagggaactc atccaaggcg agcgcacggc agaggtcgag cttcccgccg gccgcgccgt
     2221 atggctcccc gcccaatcgc acgctggcca caacatcggc gagacggaca cggacgtgtt
     2281 ctttgtcgag ctcaaggaac cgtcgcctgt cgacgccgct gccgcagggc gcgctctggg
     2341 cccggtggac tagccctcaa gggcttcacc acacacgtcg tggtccgccc gccctggggt
     2401 ccttggcgac gacgcgtcgg cgcgccggtg cgccggtgcg gcaactcaag cccgtgacca
     2461 gtcgcacgcg gcgtgcaggc ctcctcatca ggggttttgg caagtgccgg gggctgccgt
     2521 cccgaagcga ctctcgatcc gtgttcgatg tccgaagagc ccggctacaa taatgtggat
     2581 acgtcaacgg acggcgctgt cgtccaccta tttcgtgtgg aggcggcagt gcgagccacg
     2641 ctcaaaaaga atcccgtgga catcgaagcg atgttcgacg gcgtcgttgg acgctacgac
     2701 atcaccaaca cgatcatcac tgccggtcac gacaactact ggcgttggcg cgccactcgc
     2761 gcccttggct ctgggccggg cgacaccgtg ctggatgtcg ccgcgggcac cgggcgctcg
     2821 acgcgcagtc tccagcgagc cggcgcc
//
LOCUS       DASRKH010000179         3123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_35440, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000179 DASRKH010000000
VERSION     DASRKH010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35440"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /locus_tag="VFC06_02990"
     CDS             complement(<1..992)
                     /locus_tag="VFC06_02990"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HZL80898.1"
                     /translation="MPHYLDHAATTPMRPAAREAWIEASLRVGNASSLHASGRSARSL
                     LEDARERVAAALGAHPTEVIFTSGGTEADNLAIKGAFWARRDADPARVRVVTTAVEHH
                     AALDPVRWLAAHQGAEGVELPVDDHGVLRIDALATELEAHADSTALISTQWVNNEMGA
                     IQPIAKVVALARETGIPVHCDAVQGIGHLAFDFSASGLATAAVSSHKVGGPVAVGTLL
                     ARRGAALAPLTHGGGQERRLRSGTVDVAAAWAFAVALDEAVAAVEAEAARLAALGGRL
                     AQAIVSLAPGATVHGPREGAPHIVHAIIPGASSEAMLVALDLAGVEVSPGAACTA"
     gene            1062..1889
                     /locus_tag="VFC06_02995"
     CDS             1062..1889
                     /locus_tag="VFC06_02995"
                     /inference="COORDINATES: protein motif:HMM:NF004824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HZL80899.1"
                     /translation="MATWFITGCSTGIGRAAAEAVADAGHTVVATARRPETLDDLVAA
                     HPDTAIALPLDVTDDASVATAVDAALERVGHIDVLVNNAGIGYFSTIEESDEADVRTM
                     MEANFWGAARVTTALLPHLRERRAGHIFTVSSIAGVRGSAGLGYYCATKFAVSGFMEA
                     LAAEVGHLGIKVTLIEPGPVRSLWIPSGEKKDDVLPDYADVMEPFWDRIRGLPGKQPG
                     SPEALAEAMLDLADAPVPPLHFIAGAHALAGARTKIERLTVEIDAWEDLTRSVDRPA"
     gene            complement(1905..2864)
                     /locus_tag="VFC06_03000"
     CDS             complement(1905..2864)
                     /locus_tag="VFC06_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HZL80900.1"
                     /translation="MTDTIVAVLVEHRGNEIAQPTFEALTLARSIGSPVAVWMGEEPP
                     ADAVTALGEYGAAEVRVLAIGERARHPKTASAVLAAATGDAALVLVVSTFLNKEIATH
                     LALATGAGVVVDASGAALVDGRIEAQQTVFAATWNVRTRINADRAIVAVRPNTTQATP
                     ADTAGAAEVVGVLAVLPELRETIVSVTPVTREEGVPLSEAAVVVAGGRGTSGDYTLVR
                     ELADVLRGAVGASRDATDEGWISHEHMVGQTGVTVTPVLYVACGISGAVHHRGGMQAS
                     GTIVAVNIDPDAPIFEIADFGVVGDLHDVLPQAIEALKAHRQG"
     gene            complement(2861..>3123)
                     /locus_tag="VFC06_03005"
     CDS             complement(2861..>3123)
                     /locus_tag="VFC06_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010149099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HZL80901.1"
                     /translation="LVSVTDEANKPRYPNFKLIMAARTKPVATWSLADIGVDPASVGA
                     LGSRTTVIDASPQPPRENRVQVTDKGDGGTQLAQFLIERGLA"
BASE COUNT          468 a         1141 c         1067 g          447 t
ORIGIN      
        1 gccgtgcagg ccgctccggg agatacctcg accccggcaa gatccagggc gaccagcatg
       61 gcctccgagg aagcgcccgg aatgatcgca tgcacgatgt gcggggcgcc ttcgcgcggc
      121 ccgtggacag tggccccggg tgccagcgag acgatcgcct gcgcgagccg gcccccgagc
      181 gcggccaacc gagccgcctc cgcctcgacc gccgccaccg cctcgtcaag cgcgacggcg
      241 aaggcccacg ccgccgcgac gtcgaccgtg ccagaccgca gccgccgctc ctgcccgccg
      301 ccgtgggtga gcggcgccaa cgcggcgccg cggcgagcca gcaaggtccc aacggcgacc
      361 ggcccgccca ccttgtggga actgacggcg gccgtggcaa gcccactcgc ggagaagtcg
      421 aaagcaaggt gcccgatgcc ctggaccgcg tcgcagtgca ccggaatgcc ggtctcccga
      481 gccagcgcca ccaccttggc gatcggctgg atcgcgccca tctcgttgtt gacccactga
      541 gtcgagatca gggcggtcga gtcggcgtgc gcttcaagtt cggtcgcgag cgcgtcgatc
      601 cgtagcaccc cgtggtcgtc caccggaagc tcgacaccct ccgcgccttg gtgagccgcg
      661 agccaacgca ccgggtcgag tgcggcatgg tgctccaccg cggtggtgac cacgcgcacg
      721 cgggcggggt cggcgtctcg acgcgcccag aacgcgccct tgatcgccag attgtccgcc
      781 tccgtgccgc ccgacgtgaa gatcacctcg gtggggtgcg ctcccaacgc ggccgcaacc
      841 cgctcgcgag cgtcctcaag caaggaccgc gcggaacgtc ccgatgcgtg caggctcgac
      901 gcattgccca ccctcagcga cgcctcgatc cacgcctcgc gcgcggccgg acgcatcggc
      961 gtggtcgcgg cgtgatcaag gtagtggggc acgggaccat cgtaggtgga ggcgcattcc
     1021 gggtagcgtg acagcagacc ccccgcggaa ggactccccc catggctacc tggttcatta
     1081 ctggctgctc caccggcatc ggccgcgcgg cggcagaggc ggtcgcagac gccggccaca
     1141 ccgtcgtcgc gaccgcgcgc cgcccggaga ccctcgacga tctggtcgcg gcgcatcccg
     1201 acaccgcgat cgcgctgccc cttgatgtca ccgacgacgc ctccgtggcg acggccgttg
     1261 acgccgcgct ggagcgcgtt ggtcacatcg acgtgctggt caacaacgcg ggcatcggct
     1321 acttctcgac catcgaggag tccgacgagg cggacgtgcg gaccatgatg gaggccaact
     1381 tctggggcgc cgcgcgggtc acgacggccc tgcttccgca cctgcgcgag cgccgcgcgg
     1441 gtcacatctt cacggtctcc tcgatcgcgg gcgtgcgtgg ctccgccggc ctgggctact
     1501 actgcgccac caagttcgcg gtgtccggct tcatggaggc ccttgccgcc gaggtgggcc
     1561 acctggggat caaggtcacg ctgatcgagc ccgggcccgt gcgctcgctg tggattcctt
     1621 ccggcgagaa gaaggacgac gtcctccccg actacgccga cgtgatggag cccttctggg
     1681 accgcatccg cggactaccc ggcaagcagc ccggctcccc agaggccctc gccgaggcga
     1741 tgcttgacct cgccgacgcg ccggtgccgc ccctgcactt catcgcgggc gcccacgcgc
     1801 tcgcgggcgc gcgcaccaag atcgagcgcc tcacggtgga aatcgacgcc tgggaggacc
     1861 tcacgcggtc ggtggacagg cctgcctagc acacgacgcc gggcctaacc ctggcggtgc
     1921 gccttgagcg cctcaattgc ctgtggcagc acgtcgtgga ggtctccgac gacgccgaag
     1981 tcggcgatct cgaagatggg cgcatcgggg tcgatattca ccgcgacgat cgttcccgag
     2041 gcctgcatgc cgccgcggtg gtgcaccgca ccggagatcc cgcacgcgac gtagagcacg
     2101 ggagtgacgg tcacgccggt ctggcccacc atgtgctcgt gactgatcca gccctcgtcg
     2161 gtggcgtcac gagaggcgcc tacggcgcct ctcagcacgt ccgccagttc acgcaccagg
     2221 gtgtagtcac cgctcgtgcc gcgcccaccg gcgaccacga ccgcggcctc cgagaggggc
     2281 acgccctcct cccgcgtcac gggggtgacc gacacgatgg tctcgcgcag ttccggcaga
     2341 accgcgagca cgccaacgac ctcggcggcc ccggcggtat cggccggggt ggcctgggtg
     2401 gtgttgggcc gcacggcaac gatcgcgcgg tccgcgttga tgcgggtgcg cacgttccac
     2461 gtcgccgcaa agaccgtctg ttgcgcctcg atgcgcccgt caaccaaggc ggcgcccgag
     2521 gcgtccacga ccacgccggc gccggtcgcg agcgcaaggt gtgtcgcaat ttccttgttg
     2581 aggaaggtcg acacgacgag cacgagtgca gcgtcgccgg tcgccgccgc cagtaccgcc
     2641 gacgcggtct tggggtgacg cgcccgctcg ccaatcgcga ggacgcgcac ctccgctgcg
     2701 ccatattcgc ccaaggcggt cacggcatcg gccggaggct cctcacccat ccagacggca
     2761 accggcgacc cgatcgatcg cgccagtgtc agcgcctcga acgtcggctg ggcaatctcg
     2821 tttccgcggt gctccacaag cactgccacg atcgtgtcgg tcatgccagc cccctttcga
     2881 tgaggaactg cgccagttgc gtgccgccgt cgcccttgtc ggtgacctgc acacggttct
     2941 cccggggcgg ttggggcgaa gcgtcgatca ccgtggtgcg cgacccgagg gcgccgacgc
     3001 tcgcgggatc cacgccgatg tcggcgagcg accacgtggc gaccggcttg gtgcgggcgg
     3061 ccatgatgag tttgaagttg gggtagcgag gcttgttggc ttcgtcggtg accgagacga
     3121 gcg
//
LOCUS       DASRKH010000180         2146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_66007, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000180 DASRKH010000000
VERSION     DASRKH010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66007"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VFC06_03010"
     CDS             complement(<1..225)
                     /locus_tag="VFC06_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZL80902.1"
                     /translation="MAYSDLAALLAMVAAGVGDAVGPAMLRAVTTHRHPDHLGALAEL
                     VRRTGVRHTPGGIALALANAGQTHLLVGDPL"
     gene            284..>2146
                     /gene="uvrA"
                     /locus_tag="VFC06_03015"
     CDS             284..>2146
                     /gene="uvrA"
                     /locus_tag="VFC06_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HZL80903.1"
                     /translation="MDDRLLVSGAREHNLKNVSLELPRNRLIVFTGLSGSGKSSLAFD
                     TIFAEGQRRYVESLSSYARQFLGQMDKPHVDFIQGLSPAVSIDQKATNRNPRSTVGTI
                     TEVYDYLRLLFARAGTQTCPVCGERITAQTAQQIVDRLTALPEGTRFQLLAPIVRGRK
                     GEYTELFAELQSKGFARAKVDGQVVPLSDPPALEKNTKHTIDVVVDRLVVREGSRQRF
                     TDSVETALLLAEGQAVADFVDVDEHHPDRQLRFSERRACPNEHPLALAEIEPRTFSFN
                     APFGSCPECSGLGTHLEVDPELVVPDEDRTLAEGAISVWPGEKSQYYFRLVSALATDL
                     KFATDVPWRALPERAREALLYGKDYEVHVKFRNRWGRDRSYTTGFEGAVHYINRKHGE
                     TESDSSREKYQGFMREVPCPSCKGARLRPEVLAVKVGGLSIAELCELSIDTAKEFLDH
                     VELTEREASIAAQVLREVHARLGFLLDVGLDYLTLSRAAGTLSGGEAQRIRLATQIGS
                     GLVGVLYVLDEPSIGLHQRDNHRLIETLTRLRDLGNTLIVVEHDEDTIRAADWVVDIG
                     PGAGEHGGEIVHSGSYAGLLAAKASLTGDYLSGRRQIPMPAVRRPRDPDRVLTVT"
BASE COUNT          342 a          753 c          723 g          328 t
ORIGIN      
        1 gagcgggtcc ccgacgagga ggtgggtctg gccggcattc gcgagcgcga gtgcaatgcc
       61 gccgggagtg tggcgcacac cggtacgacg cacgagttcc gccagcgcgc cgaggtggtc
      121 cgggtgacgg tgcgtggtga cggcccgaag catggccggc ccgacggcgt cgcccactcc
      181 cgccgcgacc atcgcgagca acgcggcgag gtccgagtac gccatgcggg acactccttc
      241 gaacaattgt gcgaactggg cggtcgtggc ctactatttc acagtggatg atcgattgct
      301 ggtcagcggg gcgcgcgagc acaacctgaa gaatgtctcc cttgaactgc cacggaacag
      361 gctcatcgtg ttcaccgggc tatccgggtc gggcaagtcg tcgctcgcgt tcgacaccat
      421 cttcgccgag gggcagcggc gctacgtcga gtcgctgtcc tcgtacgcgc gccagttcct
      481 cggtcagatg gacaagccgc acgtcgactt catccagggg ctctccccgg ctgtgtcgat
      541 cgaccagaag gccaccaacc gcaacccgcg ttcgaccgtg ggcaccatca ccgaggtgta
      601 cgactacctg cgcctcctgt tcgcgcgcgc cgggacacaa acctgcccgg tgtgcggaga
      661 gcggatcacg gcgcagaccg cccagcagat cgtcgaccgg ctgaccgccc tcccggaggg
      721 cacccgcttc cagctcctcg cccccatcgt gcggggccgc aagggcgagt acaccgagtt
      781 gttcgcggag ttgcagtcga agggcttcgc ccgcgccaag gtggacgggc aggttgtccc
      841 actctccgac ccgcccgccc ttgagaagaa caccaagcac accatcgacg tcgtcgttga
      901 ccggctggtc gtgcgcgagg gcagccggca gcgcttcacg gactccgtgg agaccgccct
      961 tctcctcgcc gagggacagg ccgtggcgga tttcgtcgac gtggacgagc accacccaga
     1021 ccgccagttg cggttctcgg agaggcgcgc gtgcccaaac gagcaccccc tcgcgctggc
     1081 cgagatcgag ccccggacgt tctcgttcaa cgcgcccttc ggctcatgtc ccgaatgttc
     1141 cgggctgggc acccaccttg aggtggaccc ggagctcgtg gtccccgatg aggaccgcac
     1201 cctcgcagag ggcgcgatct ccgtgtggcc gggggagaag tcgcagtact acttccggct
     1261 ggtctccgcg ctggccacgg acctgaagtt cgccaccgat gtcccgtggc gtgcgctgcc
     1321 ggagcgcgcg cgggaggccc tgctttacgg caaggactac gaggtgcacg tcaagttccg
     1381 caaccgctgg ggccgcgacc ggagctacac caccggtttc gagggcgccg tccactacat
     1441 caaccggaag cacggcgaga cggagtccga ctcctcccgc gagaagtacc agggcttcat
     1501 gcgggaggtg ccgtgcccga gctgcaaagg cgcgcggctg cggcccgagg tgcttgccgt
     1561 gaaggtcggc gggctgtcca tcgcggagct ctgtgagctg tccatcgaca ccgcgaagga
     1621 gttcctcgac catgtcgagc tcacggagcg ggaggcctcg atcgccgcgc aggtgctcag
     1681 ggaggtccac gcccgcctcg gcttcctgct cgacgtgggc ctcgactacc tcacgttgtc
     1741 ccgcgcggcc ggtacgctct ccggcggcga ggcgcagcgc atccggctcg ccacccagat
     1801 cggctcgggc ctcgtgggag tgctgtacgt cctcgacgag cccagcatcg ggctgcacca
     1861 gcgggacaac caccggctca tcgaaacact gacccggctg cgtgacctcg gcaacaccct
     1921 gatcgttgtc gagcacgacg aggacaccat ccgcgccgcg gactgggtgg tggacatcgg
     1981 gcccggcgcc ggggagcacg gcggcgagat cgtccactcc ggtagctatg cggggctgct
     2041 cgccgcgaag gcctcactca ccggggacta cctgtcgggc cgtcgccaga tcccgatgcc
     2101 cgccgtgcgc cgtccgcggg accccgaccg ggtcctcacg gtgacg
//
LOCUS       DASRKH010000181         3139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_62547, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000181 DASRKH010000000
VERSION     DASRKH010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62547"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="VFC06_03020"
     CDS             <1..620
                     /locus_tag="VFC06_03020"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HZL80904.1"
                     /translation="RELHDDILQSIAVATGRIDSVAAAGLPEETGSRLLDIHKILTDA
                     MANLRRYCRDLRPSLLDDLGLAEAIDWLVGDLGRRTGMDVDLIVEGTLQRLKGRDELL
                     VFRIVQEALHNVERHSEASEARVWLIFSEHHLRAAVTDNGRGMAPPDRDGDERFGAGL
                     GIRGMDERTKLLRGRLTFTSTRGQGTTLILTVPLRERAPAAGAPA"
     gene            complement(1010..1324)
                     /locus_tag="VFC06_03025"
     CDS             complement(1010..1324)
                     /locus_tag="VFC06_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HZL80905.1"
                     /translation="MTAIDPIHPGEVPLEEFLEPLGVSQNRLAVAIGLPPRRINEIVH
                     GKRRVTADTALRLSRYFGTSARFWLNLQTRYDLEIEKDQLGASLDETHPFAETHAGPP
                     AA"
     gene            complement(1906..2484)
                     /locus_tag="VFC06_03030"
     CDS             complement(1906..2484)
                     /locus_tag="VFC06_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80906.1"
                     /translation="MPVIEARLPFPLTGLDCDNGGEFINHALIGWAADRDIYFTRARP
                     YRSNDNAHVEQKNGDVVRRHAFHYRYDTTTELRLLNELYALVRLRLNLFTGTTKAIGY
                     RANRNGRNIRVYDKPRTPYQRVTDTGILAPATHTELQELFASLNPAQLTRDITAIQTQ
                     LIHLAAAKTTAHTTSVSRAKPREARTHDSRAS"
     gene            complement(2378..>3139)
                     /locus_tag="VFC06_03035"
     CDS             complement(2378..>3139)
                     /locus_tag="VFC06_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80907.1"
                     /translation="GARREITKKYAREYASASKKVRGRLLDELVATTGWSRANARRQV
                     AAAGQRKGPRRAVARAPRPRTYGYDTLKLLIEVWTLAGQPSGKYLAATMGLWLPKLIE
                     HGELDTARLNDHTQAQLLAVSGATIDRLLKPTRDAMRLTGLSGTKPGPLLRTSITVRK
                     AGDEHELVPGFVEIDLVLHCGASLQGEFLRTMTVTDVHTGWTGEHRAAQRGPPVGPRS
                     DARDRGAAPVPADRARLRQRRRVHQPCPDRLGRGP"
BASE COUNT          435 a          997 c         1151 g          556 t
ORIGIN      
        1 cccgggaact gcacgacgac atcctgcagt cgatcgcggt cgccacgggg cgcatcgatt
       61 ctgtcgcagc cgccggactg cccgaggaga ccggttcgcg gctgctcgac atccacaaga
      121 tcctcaccga cgccatggcg aacctccgac ggtactgccg ggatcttcgg ccctccctgc
      181 tcgacgacct cgggttggcg gaggcgatcg actggctcgt tggcgacctc ggtcgccgca
      241 ccggcatgga cgtcgacctc atcgtcgagg gcaccctgca gagactgaaa ggtcgtgacg
      301 agctgctcgt cttccgcatc gtccaggaag cactccacaa cgtcgagcgg cactccgagg
      361 ccagcgaggc gcgcgtctgg ttgatcttct ccgagcatca cctgcgcgcc gctgtcacgg
      421 acaacgggcg tggcatggcg cctcccgatc gcgacggcga cgagcgtttc ggtgcggggc
      481 tgggcatccg cggcatggac gagcggacga aactgctccg cggccgactc accttcacta
      541 gcacccgcgg acagggaacc accctgatcc tcacagtccc gctgcgcgag cgcgctccag
      601 ccgcgggtgc accggcctga ccggcacggc accgaccagt ggcccactcg ggcaggttcc
      661 cgtgtgaacg gaggccagtt cacggtggcg gtgcgctggc agatgacagg tggttgtgcc
      721 ggcggaggat gctgacctcg ccccggtctt ccgagcagtc tgctcggccg cggcccggtc
      781 ggtgtcctgg cggcgggcct ggttctgcgg ggcgcggacg caggcgtggg gccgggcaag
      841 ggtgcgccaa ctggtcgcgg tcttgaccgg cccgaggacc atggcgggcg ggcggaaggg
      901 ccacacgatc tccgtggccc ttcgccgcga cacggtggca tgagacctcg cgttctggct
      961 gtacgggtga gcatggcggg tctgcgtgtt cggcgcgtcg gccgggtcgc taagccgccg
     1021 gtgggccggc gtgggtctcg gcgaagggat gagtctcgtc gagggaggcc ccgagctggt
     1081 ccttctcgat ctcgaggtcg tatcgggtct gcaggttgag ccagaagcgg gcgctcgtgc
     1141 cgaagtagcg ggaaaggcgc agggcggtgt ccgcggtgac ccggcgcttg ccgtggacga
     1201 tctcgttgat ccggcggggc gggaggccga tcgcgactgc cagccggttc tgactcacgc
     1261 cgagaggctc gaggaactcc tcaagcggga cctcgccggg gtggatcggg tcgatggcgg
     1321 tcatggtcac ctcctggtgg tagtcgacga tctcgacgtc gtggggccct gcgtccgtcc
     1381 aggtgaagcc gatccgccac tgctgattgg tccgtttgcc gtgctgaccg gaccgctgtt
     1441 agacggcgct acgtggggga gtggtgcacg actcgggatg ctcggagcac cacgtgccga
     1501 agtctgggca gttgcgggtg aggcgtcgtt accggcggaa agcgtccatc tcgctttggt
     1561 cgcgccaacc cggaccctcg gaacccaacg aagcggtcgg gaggtgccgg gcccgtggcg
     1621 ggaatgcggg tgcggctcag atcgtcccga gatcctggcc acgctggggg aggcggtcgc
     1681 gcagtcgcct tagaacactg gatggaggag gcgaacatca ggttgatggg gtggtcgaag
     1741 ttctcaacgt tcgcccgaca caggtcgcgg ttgtccgcgt cggctccgag tggctcgtcc
     1801 gaggccctgc gtctggcgcc atgaggttgt gcgggtggtg ctactcaacg acgacctcct
     1861 tctcccgtgt gccgggttga cgccgggcag ccgtagtgcc tcatgtcaag atgctcgcga
     1921 atcatgtgtg cgtgcctcac gtggttttgc tcgcgaaacg ctggtggtgt gtgcggtggt
     1981 cttggcggcg gcgaggtgga tcagctgggt ctggatcgcg gtgatgtccc gggtcagttg
     2041 ggcggggttg aggcttgcga agagttcctg aagctcggtg tgcgtggcgg gggcgaggat
     2101 gccggtgtcg gtgacgcgct ggtagggggt gcggggcttg tcgtagacgc ggatgttgcg
     2161 cccgttccgg ttcgcccggt atccgatggc tttggtggtc ccggtgaaca ggttcagccg
     2221 gagcctgacg agggcgtaga gctcgttcag cagccgcagt tcggtggtgg tgtcgtaccg
     2281 gtagtggaac gcgtgccggc gtacgacgtc cccgttcttc tgttcgacgt gggcgttgtc
     2341 gttggaccgg taggggcggg cacgggtgaa gtagatgtca cggtccgcgg cccagccgat
     2401 cagggcatgg ttgatgaact cgccgccgtt gtcgcagtcg agcccggtca gcgggaacgg
     2461 gagccgcgcc tcgatcacgg gcatcgcttc gaggacccac cggtgggccc cgttgcgcag
     2521 cgcgatgttc tccggtccac ccggtgtgca cgtcggtgac ggtcatggtg cgcaggaact
     2581 cgccctgcag cgaggcgccg cagtgcagca ccaggtcgat ctcgacgaac ccgggcacca
     2641 gttcgtgctc gtcgccggct ttgcggacgg tgatcgaggt gcgcagcaac ggacccggct
     2701 tcgtgcccga gagcccggtc aaccgcatcg cgtcccgggt gggtttgagc agtcggtcga
     2761 tcgtcgcccc ggacacggcc agcagctgcg cctgggtgtg atcgttcaac cgtgcggtgt
     2821 cgagctcacc atgctcgatc agtttgggca gccacagacc catcgtggcc gcgaggtact
     2881 tccccgaggg ctggccggcg agcgtccaca cctcgatcag cagcttcagc gtgtcatacc
     2941 cgtacgtccg cggccgcggc gcccgcgcca ccgcccgccg cggacccttc cgctgccccg
     3001 cggcggccac ctgccggcgg gcattcgccc gcgaccagcc cgttgtcgcg accagctcgt
     3061 ccagcaaccg cccccggacc ttcttcgacg cggacgcgta ctcgcgggcg tacttcttcg
     3121 tgatctcacg cctggcacc
//
LOCUS       DASRKH010000182         2472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_6519, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000182 DASRKH010000000
VERSION     DASRKH010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6519"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VFC06_03040"
     CDS             <1..293
                     /locus_tag="VFC06_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007032582.1"
                     /note="ATP-binding protein; PstABCS is an ATP dependent
                     phosphate uptake system which is responsible for inorganic
                     phosphate uptake during phosphate starvation; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HZL80908.1"
                     /translation="SLAVRPRVLLMDEPCSALDPTSTRVIEDTMTELAREVTIVIVTH
                     NMQQAARVSQQCAFFLAAEGTPGVIVEHGDTSAMFGSPLDQRTADYVGGRFG"
     gene            432..1577
                     /locus_tag="VFC06_03045"
     CDS             432..1577
                     /locus_tag="VFC06_03045"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HZL80909.1"
                     /translation="MALRLGTVNGRTRAAVATLAAAVLLVGSATTVLADPTAPSDRDV
                     RAAQQAVTAAQMSVAEIEVRLAQQSVERDAAVVAVQAAGEKYTQAESDRLAAADTATE
                     AATRSAAADAQAETARRSLVAIARQTARSGGSMDTIEAVLSADGFRQVVDRSTSAAVE
                     RARQDQLDADRQARADAAAEAQRRAATPAAPAVAAPAVTTVAAPKPATTTPPTTTTTT
                     PPAAAAPAVTPPAAPVVSDPLPPAPTPTGAYGLGTGRSRGTADQGAAAVAWASAQVGL
                     PYVYGSAGFESFDCSGLTMRSWQAAGVNLGRTSRDQYKQVLKISYDDMRPGDLVFYST
                     DQNNPDAIYHVAMWIGGNQVVEAPRAGVPVRITSMRWAATMPFAGRA"
     gene            complement(1665..2162)
                     /locus_tag="VFC06_03050"
     CDS             complement(1665..2162)
                     /locus_tag="VFC06_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013115868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HZL80910.1"
                     /translation="MEFDVTIEIPKGQRNKYEVDHATGRIRLDRMLFTSTRYPDDYGF
                     IEGTLGEDGDPLDALVLLEEPTFPGCLIRCRALGMFRMRDEAGGDDKVLCVPTGDQRA
                     AWRQDIDDVSDFHRLEIQHFFEVYKDLEPGKSVEGAHWTDRVEAEAEIERSRQRAIDT
                     GYYLH"
BASE COUNT          356 a          904 c          871 g          341 t
ORIGIN      
        1 ggtccctcgc ggtacgcccg cgcgtgctgc tcatggacga gccgtgctcg gcgctcgacc
       61 cgacgtcgac gcgcgtcatc gaggacacga tgaccgagct cgcgcgcgag gtcaccatcg
      121 tgatcgtcac gcacaacatg cagcaggccg cccgggtctc gcagcagtgc gcgttcttcc
      181 tcgctgccga ggggacgccg ggggtcatcg tcgagcacgg tgacacgagc gcgatgttcg
      241 gttcgcccct cgaccagcgc accgcggact atgtcggggg acgtttcggg tagttcgtcc
      301 agaaccacaa ccgtgtgact cgtgtgacag gtgtgactgg tgttcacttc tgggatctgc
      361 gcaggtagcg tgccctacgg gcctcctccc gaggcctcaa ggttcaccgc gcggcagacg
      421 aaggacgtga cgtggcactt cgactcggaa cggtcaacgg acgcacgcgc gcggcagtcg
      481 cgacgctggc ggccgctgtc ctcctcgtcg gctccgcgac cacggtcctc gcggacccga
      541 cggcgccgag cgaccgggac gtgcgcgccg cgcagcaggc ggtcaccgcg gcgcagatgt
      601 cggtcgccga gatcgaggtg cggctcgcgc agcagagcgt cgagcgcgac gccgcggtcg
      661 tcgcggtcca ggcggccggt gagaagtaca cgcaggctga gtctgatcgg ctcgccgccg
      721 ccgacacggc gaccgaggcc gccacgcggt ccgccgcggc ggacgcccag gccgagaccg
      781 cacgccgctc gctcgttgcg atcgcccggc agacggcgcg gtcaggcggc tcgatggaca
      841 cgatcgaagc agtcctgtcc gccgacgggt tccggcaggt cgtcgaccgc agcacgagcg
      901 cggccgtcga gcgcgcacgc caggaccagc tcgacgccga ccgccaggct cgcgcggacg
      961 cagccgccga ggcgcagcga cgcgcggcca cccccgctgc tccggcggtc gctgcccccg
     1021 cggtgaccac cgttgcggcg cccaagccgg cgaccacgac gccgcccacg acgaccacga
     1081 cgacgccacc cgcggccgcc gcacctgccg tcaccccacc agccgccccg gtcgtctcgg
     1141 atccgttgcc tcccgcgccg accccgacgg gcgcgtacgg gctcggcacg ggcaggtcgc
     1201 gcggtacggc ggaccagggg gcggctgccg tcgcctgggc atcggcgcag gtgggcctgc
     1261 cctacgtcta cggctcggcg gggttcgaga gcttcgactg ctcaggcctg acgatgcgct
     1321 cgtggcaggc cgccggtgtc aacctcggca ggacctcacg cgaccagtac aagcaggtcc
     1381 tcaagatcag ctacgacgac atgcgcccgg gtgatctggt cttctactcg accgaccaga
     1441 acaaccccga cgcgatctac cacgtcgcga tgtggatcgg cggcaaccag gtcgtggagg
     1501 ctccgcgcgc aggcgttccg gtccggatca cctcgatgcg ctgggccgcg acgatgccgt
     1561 tcgccggacg cgcctgaccc gaggactgct cgccccgccg gcaggagcca cgcggcgcgg
     1621 gcacacgcag gtccggcacg gttccccgcg ccggaccgga cagctcagtg caggtagtac
     1681 ccggtgtcga tcgcgcgctg acgcgaccgc tcgatctcgg cctcggcctc gacccggtcg
     1741 gtccagtgcg cgccctccac ggacttaccc ggctcgagat ccttgtacac ctcgaagaag
     1801 tgctggatct cgaggcggtg gaagtcggac acgtcgtcga tgtcctgacg ccacgcggcg
     1861 cgctggtcgc ccgtcggcac gcacaggacc ttgtcgtcgc cgccggcctc gtcgcgcatg
     1921 cggaacatgc cgagagcacg acaccggatc aggcaaccgg ggaacgtcgg ctcctcgagg
     1981 agcacgagcg cgtcgagcgg gtcaccgtcc tcaccgaggg tgccctcgat gaagccgtag
     2041 tcgtcggggt accgggtcga ggtgaagagc atgcggtcga ggcggatccg cccggtcgcg
     2101 tggtccacct cgtacttgtt gcgctgcccc ttggggatct cgatcgtgac gtcgaactcc
     2161 acagctctcc tccgcaggtg cgaggtcgtc gcgccgcgtc agcacgagtc ggcacgcacg
     2221 cgtgtccgca gggcgggtcg cggtgcggcc cgccgctctt gtgtgcttag tgtgacgcac
     2281 cggagggcct ggtgcgcccg cccggacggg acggagagga cgggacatgg catcgggagt
     2341 gcgcgtggtc gccacgacgg cgctcgtcgt cgttctagcg ggcggcgcgt acgcgacggc
     2401 cgacgcctac gacgtcgtgc cgggcatggt cacgctcgcc cccgtcccgg cgcccgcgaa
     2461 cccgttcccg ac
//
LOCUS       DASRKH010000183         2493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_44713, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000183 DASRKH010000000
VERSION     DASRKH010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44713"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1659)
                     /locus_tag="VFC06_03055"
     CDS             complement(<1..1659)
                     /locus_tag="VFC06_03055"
                     /inference="COORDINATES: protein motif:HMM:NF022113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqB family beta-propeller domain-containing
                     protein"
                     /protein_id="HZL80911.1"
                     /translation="MRRAWVLFAACLALAACTSIPTSGGVQAGDSDVVQPGPIFPFLQ
                     GPAPNATPRSIVQGFLTASAGGSVSGFDVAREFLTGPAAIDWDPLANVTVFDSRQVVP
                     SYNEDTGIFTYSVPVAAQVDPSGVMVTAAADVRQDLEFHVGTDEFGRYRIDGLDGGIV
                     MSAADFDRFFRPVSLYFAAADGTTMVPDVRWFSNNDQIATATARELVAGPSVWLAGGV
                     QTGFPPGSALGVESVVVNDGVASVALALGSAGDPTQRSLAQQQMLKTLTQLPAVQNVV
                     TTVGAVPIGGDDSVTLEVAALPGERALVVADERLGLWDGETVSVTAPDMGVVPASAHG
                     FALGYEGATVAFVQDGSVQISTALAAQEGLVSAAEGAPPTPDAVVPSTELIPGTAMVA
                     PSFDANGWVWSGEAASKGVLVVATPGQDVAEIDAAWLAGSSIQSVAVSRDGARMAILS
                     RSGVEQVLEVVSIARDEDGRPLAVGKPLGVGSTVRLSIDLEWVDDTSVVALGQESGEM
                     SLAQIGGWNTDFTSYTGATAITARNGVRTLLAVASKGELVARSGN"
     gene            complement(1656..>2493)
                     /locus_tag="VFC06_03060"
     CDS             complement(1656..>2493)
                     /locus_tag="VFC06_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZL80912.1"
                     /translation="FNDMATSLQRQITRMEDLSRLQRRFVSDVSHELRTPLTTVRMAS
                     DVLHEARADFAPSVARSAELLSTQLDRFEALLADLLEISRIDAGAAQLDFEDHDLGDV
                     VRDQVETLALAASDQHVQLRLWMQEGPHIASMDSPRVGRIVRNLLSNAIEHAQEGPVD
                     IAVSSTARTVAVVVRDYGVGLSASQVQRVFDRFWRGDPARARTMGGTGLGLSIAREDA
                     LLHDGVLEVSGRPGEGASFRLMLPSGSRGLGLREPLPLDMNEADFPEVGRRVSLASEA
                     EL"
BASE COUNT          450 a          881 c          804 g          358 t
ORIGIN      
        1 gttgcccgac ctggccacca actcgccctt agacgccacc gcgagcaggg ttcgcacccc
       61 gttgcgcgcc gtgatggccg tcgcgcctgt gtacgacgtg aagtcggtgt tccaaccgcc
      121 gatctgggcc aacgacatct ccccagactc ctggccaagg gccactaccg acgtgtcatc
      181 cacccattcc agatcgatgg acagcctgac agtggacccg acgcccagcg gcttgcccac
      241 cgcgagcggc cgtccgtcct cgtcacgcgc gatcgacacc acctcgagca cttgctccac
      301 gcccgaccga ctgaggatcg ccatccgggc gccgtcacga gagacagcca cagactgaat
      361 cgaggacccg gcaagccacg cggcgtcgat ctccgccaca tcttggcccg gagtcgcgac
      421 aaccaacacc cctttggagg cggcctcgcc gctccatacc cagccgttgg cgtcgaacga
      481 cggcgcgacc atcgccgtgc ccggaatgag ttcggtggac ggaaccacgg cgtcaggcgt
      541 gggaggcgca ccctcggcag ccgacaccag gccctcctgc gccgcgaggg cggtgctgat
      601 ctgcacggag ccgtcctgga caaaggcaac ggtggcgccc tcatacccca gcgcgaagcc
      661 atgcgcgctt gcaggcacca cccccatatc gggcgctgtg acggacaccg tttctccgtc
      721 ccagagaccg agccgttcgt cagcaacaac aagcgcgcgc tcccccggaa gtgcggccac
      781 ctcaagcgtg accgagtcgt cgccgccgat cggcacggca cccaccgtcg tcaccacatt
      841 ctggaccgca ggcaattggg taagggtctt cagcatctgc tgctgggcga gcgaacgctg
      901 ggtggggtcc ccggcggacc ccagggcgag cgccacgctc gccacgccgt cgttcactac
      961 caccgactcg acgccgagcg cggacccggg cgggaaacct gtctgcactc cacccgccaa
     1021 ccacacagac gggcccgcaa cgagctcccg cgctgtcgca gttgcgatct gatcgttgtt
     1081 ggagaaccac cgcacgtccg gcaccatcgt ggtgccgtcc gccgcggcaa agtacaggct
     1141 cacgggccgg aagaaacggt cgaagtcggc ggccgacatc acgatgccgc cgtcgagccc
     1201 gtcgatgcga tacctgccaa actcgtcggt gccaacgtgg aactcaaggt cctgacggac
     1261 gtcggcagct gccgtgacca tcactccgga gggatcgacc tgcgccgcca ccggcacgga
     1321 gtaggtgaag atgccagtgt cttcgttgta cgacggcacc acctggcgag agtcgaagac
     1381 agtgacgttc gcgaggggat cccagtcgat agcggccggg ccggtgagga attcgcgcgc
     1441 cacatcgaaa ccactgacgg acccaccggc cgatgccgtc aggaaccctt ggacgatgga
     1501 gcgtggcgtc gcgttgggcg cgggtccctg taggaacgga aaaatcggcc cgggctgaac
     1561 gacgtcggag tcccctgcct gcacgccgcc gctcgtgggg atcgacgtgc acgccgcgag
     1621 cgcgaggcat gcggcaaaca ggacccacgc gcgcctcaca gttccgcctc cgatgcaagc
     1681 gacacgcgcc ttcccacttc gggaaagtcg gcttcgttca tgtcgagcgg caacggttcg
     1741 cgaagcccaa gcccgcgaga gccgctcggc agcatgagcc tgaacgacgc gccctcacct
     1801 ggccgcccag acacctccag gacgccgtcg tgtagtagcg cgtcctcgcg agcaatcgac
     1861 agaccgaggc cggtgccgcc catcgtgcgg gcgcgggccg gatcgccgcg ccagaaccgg
     1921 tcaaagaccc gttgcacctg ggaggcggac aggccgacgc cgtagtcgcg caccacgacc
     1981 gcgacggtgc gcgcggtgga cgacaccgcg atgtcgacgg gcccctcttg cgcgtgctcg
     2041 atcgcgttgg acagcagatt gcgcacgatg cgccccacgc gcggagagtc catcgatgcg
     2101 atgtgggggc cctcctgcat ccacagcctc agctgaacat gctggtccga tgccgcgagt
     2161 gcgagggtct cgacctggtc cctcacgacg tcgcccaggt cgtggtcttc gaagtcgagt
     2221 tgcgcggcgc ctgcgtcgat gcgggaaatc tccaggaggt ccgcgagcag cgcctcgaag
     2281 cggtccaatt gcgtggacaa gagttcggct gaccgcgcga cggagggcgc gaagtcggcg
     2341 cgcgcctcgt gcaacacgtc cgatgccatg cggaccgtcg tgagtggggt gcgcagttcg
     2401 tgggagacgt ccgagacgaa ccgccgttgc agacgtgaca ggtcctccat gcgggtgatc
     2461 tgtcgctgca gtgacgtggc catgtcgttg aac
//
LOCUS       DASRKH010000184         2170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_43048, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000184 DASRKH010000000
VERSION     DASRKH010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43048"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="VFC06_03065"
     CDS             complement(<1..526)
                     /locus_tag="VFC06_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019618020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
                     /protein_id="HZL80913.1"
                     /translation="MPLAVELHGTIVGTLEGDARNFDFIPSAEGIDCFGANSAVLSVA
                     IPLVPRQRTDQSGRRRNWFAELLPEGNQYDYMLQQAGLRAGDTPGFLARYGRDVAGAL
                     QLWDLDDPTEPAEPGVRPVTAAQIRGLLEDPIGSPLANAPQVGKSSLGGVQPKIVLAR
                     TDEGWAQALGGHPTT"
     gene            complement(510..755)
                     /locus_tag="VFC06_03070"
     CDS             complement(510..755)
                     /locus_tag="VFC06_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZL80914.1"
                     /translation="MSKRTARLHAPADFGLAIQQARLERGMSQKQLAEQLAVPQSTIS
                     EIEAGKSTIYLRRLLSLARATGIEFTATWGGDDAARG"
     gene            1024..1320
                     /locus_tag="VFC06_03075"
     CDS             1024..1320
                     /locus_tag="VFC06_03075"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZL80915.1"
                     /translation="MAVKTQADIQLEVAIGDGIRRARLLANMDQATMARRANVSLSAV
                     KGLESGKGTTLLTLLRVLRVLGREDIFEQLQTQGPPRHGRYGGRERQRASARRP"
     gene            1320..>2170
                     /locus_tag="VFC06_03080"
     CDS             1320..>2170
                     /locus_tag="VFC06_03080"
                     /inference="COORDINATES: protein motif:HMM:NF025041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HZL80916.1"
                     /translation="MVFKHVDELVVSIWGNEVGVLYRNPQREGQCLFEYSPSWVRGGV
                     NLAPMGMRFNAADPQGPPAHRTDEDRATWHGIPALLADSVPDDFGNRVIDAALAREGV
                     SRGDISAMDRLAYVGERGMGALIFEPSRTHHFKPTVIDLSDLVEKARETLRGSLGATS
                     RSEALTDIISTGTSAGGARAKAVIAWNRETNEVRVGNLPLPAEATGFEQWLIKFDGIT
                     KGDYNLSDPIGQTRAEHVYALMAENAGIDMSQTLLLDEGPRSHFMTRRFDRPGTDGER
                     LHMQTLNA"
BASE COUNT          396 a          688 c          694 g          392 t
ORIGIN      
        1 gcgtggtggg gtggccgccg agcgcctggg cccacccctc atctgtgcgc gccagcacaa
       61 tctttggctg cacaccgccg agggacgact tccccacctg gggcgcgttg gcaagcggtg
      121 atccgatcgg gtcttccagc aggccacgga tctgagctgc cgtgaccggc ctgacgccgg
      181 gttccgcggg ttcggtgggg tcatcgaggt cccacagctg caaggcgccc gccacgtcac
      241 gcccgtagcg ggccaggaac ccgggcgtat cgccagcacg gagtccagcc tgctgcaaca
      301 tgtagtcgta ctgattgccc tctggaagca gttcagcgaa ccagttccgg cgccggcccg
      361 actggtctgt tcgctgccgt ggaacgagcg ggatcgcgac tgaaagcacg gcgctgttcg
      421 cgccgaagca gtctatcccc tcggcgctgg ggatgaagtc gaagttccgt gcgtcgccct
      481 caagggtgcc gacgatcgtc ccgtgaagct caaccgcgag cggcatcgtc gcctccccag
      541 gtcgcggtga actcgatccc cgtggctcgt gcgaggctca acaggcggcg cagatagatg
      601 gtcgacttcc ctgcctcgat ctcgctgata gtgctctgcg ggacggcgag ttgctcggcg
      661 agctgcttct gcgacatccc gcgctcaagc ctcgcctgtt ggatggccag cccgaagtcc
      721 gcgggggcat ggagccttgc ggtgcgcttc gacatggcgg tcccttcgtt tcgtgatttc
      781 acgataccgc atgcctatcg gaatatcacg atattcagcg cgtatcgtgt tattccgata
      841 cccggcgagc ggtgtgccgc tactgtgttc ggtggcccat tgaccccatt gaccgcacct
      901 ccctgatccg cctcctctct caggggcaaa ccctcaagat cagttgtcac caactcctgc
      961 atcagaccca agagttgaaa atgcatctac catgtcagta tatggggtgt tacagttgaa
     1021 aacatggctg ttaaaacaca ggcggacatc cagttggagg tcgccattgg tgacggcatc
     1081 cgtcgggcgc gactgctcgc aaatatggac caggcgacca tggcgcggcg tgccaacgtt
     1141 tcgctcagcg ccgtgaaggg ccttgagtca ggcaagggga ccacgcttct gacccttctg
     1201 cgcgtgctgc gcgttcttgg tcgcgaagac atctttgagc aactccagac tcaaggtccg
     1261 ccacgccacg ggcgatacgg cgggcgcgag cgacagcgtg ccagtgctag gaggccctga
     1321 tggtgttcaa gcacgtggac gaactcgtcg tgagcatctg gggcaacgaa gtcggcgtcc
     1381 tgtaccggaa cccgcagcgt gaggggcaat gcttgttcga atacagtccg tcctgggtgc
     1441 ggggcggcgt gaacctggct ccgatgggga tgaggttcaa cgcggcggac ccccaggggc
     1501 caccggcgca ccgcaccgat gaagacaggg ccacctggca cggcattccg gcgctattgg
     1561 cggattcggt gccggacgac tttggcaacc gcgtgatcga tgcggcgctg gccagggagg
     1621 gcgtatcgcg gggcgacatc agcgccatgg atcgtctcgc atatgtgggg gagcgcggca
     1681 tgggcgcgct catcttcgag ccgtctcgga cacaccattt caagccaact gtcatcgatc
     1741 tgtcagacct cgtggagaaa gcacgcgaga cgctacgcgg aagtcttggc gccacctcac
     1801 gcagcgaagc attgaccgac atcatctcga cgggcacctc ggctggaggt gcacgagcca
     1861 aggccgtcat agcctggaac cgcgagacca acgaggtgcg cgtaggcaac ctgcccttgc
     1921 ctgcggaggc caccggcttt gagcaatggc tgatcaagtt cgacgggatc accaagggcg
     1981 actacaacct gagcgacccc atcggccaga cgcgcgccga gcatgtctac gcgctgatgg
     2041 ccgagaacgc tggcattgac atgtcgcaga ctcttctgct tgacgagggc ccgcgatcgc
     2101 acttcatgac gcgccgcttc gatcggcccg gcaccgacgg cgagcgactc cacatgcaga
     2161 cgctcaacgc
//
LOCUS       DASRKH010000185         2315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_52980, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000185 DASRKH010000000
VERSION     DASRKH010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52980"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..1462)
                     /locus_tag="VFC06_03085"
     CDS             complement(44..1462)
                     /locus_tag="VFC06_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80917.1"
                     /translation="MSPTNLPLSAVLASLPDDVAAPAREALNSLLLDEGEDGEWDVVS
                     QLELQTFLWYSLPTKWLVDSREHHEVAWALGDFYRAAGKQRFADLCRAQHTHLLIDLW
                     ETDEDRARKELHRLMDLSGVKPPSIDSLTWGALMGLTETVAYHTASYALERAIEDGRL
                     TPGGRGWRGHAVRITTAALGRGHSERPALSVAGAVRQERRDHWLADVQRRGLVLTEDL
                     ADAVTAPSVDDLPDDVVTPPLWGGGGPPRLITTRSDVVALEGVAASLEPLVWFLGEIG
                     DGITLTQTGNLPRALVLAADERYDWFDLEPRSRLRSMWDLNELVALNELVTELRWVTR
                     TGQKLRLSVKGRTMRDDPLGLARAVFGYLFNQRDWHGDGAVATATVLLGAYETLTTDE
                     VYDRILHYLGTRWAREGAPLRERQDVFGASRRFDTLGAGFGWFAPLPRRDFRLHRPLN
                     AVGRAACVTGLRHVVAGPWLRM"
     gene            complement(1504..2232)
                     /locus_tag="VFC06_03090"
     CDS             complement(1504..2232)
                     /locus_tag="VFC06_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HZL80918.1"
                     /translation="MTIRIGTSGWSYDHWDGVLYPPGLPARDRLGVYTANFTTVELNA
                     SFYRWPREATFASWNRRLPPYFHMTVKAPRGLTHAKRLYSPEVWVERICRSWHALRDH
                     RGMLLVQLHPAHERDDARLDWFLSRFPDWVRIAVEFRHPSWVHDDVFRLLEHRRATYV
                     VTSGAGLPCVLRATSDTVYVRLHGPDTNHLYGGSYSDDDMRWWADRIREWDASGHDVY
                     AYFNNDGGGNAVRNAWTLRHFCGA"
BASE COUNT          355 a          786 c          840 g          334 t
ORIGIN      
        1 gggcctgtgg acaaccggcc ggtaaacccg gatcggccga ttgctacatc cgcaaccagg
       61 gtcccgccac cacatgccgc aggccggtca cgcacgccgc gcgtcccacc gcgttgagtg
      121 gccggtggag gcggaagtcc cgccgcggca gtggcgcgaa ccacccgaac ccggccccca
      181 gtgtgtcgaa cctccgcgac gccccgaaca cgtcctgccg ttctcgcagt ggcgcgccct
      241 cacgtgccca gcgcgtgccg aggtagtgga ggatccggtc gtagacctcg tccgtggtga
      301 gggtttcata ggcgccgagc agcaccgttg ccgtcgccac cgcgccgtcc ccgtgccagt
      361 cacgctggtt gaacaggtac ccgaacaccg cgcgcgccaa ccccagcgga tcgtcccgca
      421 ttgttctccc cttcacggac aaccggagct tctggccggt cctcgtcacc caccgcagct
      481 cggtgaccag ttcattcagc gcgacaagct cgttcaggtc ccacatcgag cggaggcggg
      541 agcgcggctc gaggtcgaac cagtcgtacc gctcgtccgc ggccagcacg agcgcgcgcg
      601 gcaggttgcc ggtctgcgtg agggtgatgc cgtcgccgat ctcgcccagg aaccacacca
      661 gcggctccag tgacgccgcc accccctcga gggcgacgac gtcggaccgt gtggtgatga
      721 ggcgtggcgg tccgccgcca ccccacagcg ggggcgtgac gacgtcgtcg ggcaagtcgt
      781 cgacggacgg cgcggtgacg gcgtcggcaa ggtcctcggt gagaacgagc ccgcgacgct
      841 ggacgtcggc gagccaatgg tcgcgccgct cctgccggac cgcgccggcc acgctgagcg
      901 cgggcctctc ggagtgcccc cgaccgaggg cggccgtcgt gatccggacg gcgtgccctc
      961 gccagccgcg gccacctggg gtgaggcggc cgtcctcgat cgcgcgctcg agggcatagc
     1021 tggcggtgtg gtaggcgacg gtctcggtca ggcccatcag ggccccccag gtgagggagt
     1081 cgatcgatgg tggcttgacc cccgagaggt ccatgagccg gtggagttcc ttgcgggccc
     1141 tgtcctcgtc ggtctcccac agatcgatga gcaggtgggt gtgctgggcg cggcagaggt
     1201 cggcgaagcg ctgctttccg gccgcccggt agaaatcgcc gagcgcccat gccacctcgt
     1261 ggtgctcccg gctgtccacc agccacttcg tgggcagcga ataccagagg aaggtctgca
     1321 gctcgagttg cgagaccaca tcccactcgc cgtcctcacc ttcgtcgagg agcagcgagt
     1381 tgagcgcctc ccgggcaggc gccgcgacgt cgtcgggcag ggaggcgagg acggcgctga
     1441 gcgggaggtt ggttggactc acggcacaag gacagcacat cagtgacgac cggcgcacgt
     1501 ccctcacgca ccgcagaagt gccgcagcgt ccacgcgttg cggacggcgt tgccgccgcc
     1561 gtcgttgttg aagtaggcgt agacgtcgtg gccggaggcg tcccactccc ggatgcggtc
     1621 agcccaccag cgcatgtcgt cgtcggagta ggagccgccg tagaggtggt tcgtgtccgg
     1681 gccgtggagg cggacgtaga cggtgtccga cgtcgcccgc agcacgcacg gcaagcccgc
     1741 gccgctcgtg accacgtagg tggcgcggcg gtgctcgagc aggcggaaga cgtcgtcgtg
     1801 cacccaggag gggtggcgga actcgacggc gatccgcacc cagtcgggaa agcgggagag
     1861 gaaccagtcg aggcgggcgt cgtcgcgctc gtgggcgggg tgcaactgga ccagcagcat
     1921 cccgcggtgg tcccgcaggg cgtgccagga gcggcagatg cgctcgaccc acacctccgg
     1981 ggagtagagg cgcttcgcgt gcgtcaggcc ccgcggcgcc ttcacggtca tgtggaagta
     2041 cggcggcagc cggcggttcc aggaggcgaa ggtcgcttcc cgcggccagc ggtagaagct
     2101 cgcgttcaac tccacggtgg tgaagttcgc ggtgtacacg ccgaggcggt cccgcgcggg
     2161 caggccgggt gggtagagca ccccgtccca gtggtcgtag ctccagccgg aggtgccgat
     2221 gcggatcgtc atgaggtcac tgtagggtcg ctggcatgaa cgtcaccttc gacggcgtcc
     2281 tggtccggac cgagaccagt gtggacgcgt ggcac
//
LOCUS       DASRKH010000186         2184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_22976, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000186 DASRKH010000000
VERSION     DASRKH010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22976"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VFC06_03095"
     CDS             complement(<1..263)
                     /locus_tag="VFC06_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80919.1"
                     /translation="MSAPATWSGRTATGARAVVGWWTRPAPLARVAVLRAAVYLFLGY
                     DVLFLTNDVIGHGYAPALYQPTLLGRVLPFPTPSHTTGVLLLVV"
     gene            complement(260..784)
                     /locus_tag="VFC06_03100"
     CDS             complement(260..784)
                     /locus_tag="VFC06_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80920.1"
                     /translation="MPSPPDQSPTPANPVLPHPVEPAAPVRVLGRSARTWRLAVAGAG
                     LAVLASAQLVDTNDWFPLGSLSQYASAHDPAGTVRSVHLDAVDSTGATVPVPLNPTGV
                     GVGRAEIEGQLGRILADPALLQSISDAHAALHPDEPAYVEILVVRSTRQLQGGRPVGD
                     AVLDTLTTWQVVQP"
     gene            1097..>2184
                     /gene="groEL"
                     /locus_tag="VFC06_03105"
     CDS             1097..>2184
                     /gene="groEL"
                     /locus_tag="VFC06_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013115935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HZL80921.1"
                     /translation="MAKIIAFNEEARRGIERGLNILADTVKVTLGPKGRNVVLDKKWG
                     APTITNDGVSIAKEIDLDDPFEKIGAELVKEVAKKTDDVAGDGTTTATVLAQALVREG
                     LRNVAAGANPIALKKGIEKAVEAVIAQLLLQAKDVETKEQIAATASISAGDTAIGELI
                     AEALDKVGKEGVITVEESSALGLELELTEGMRFDKGYLSAYFVTDPERQEAVLEDAYV
                     LLVESKISNVKDLLPLLEKVIQAGKPLFIIAEDVEGEALATLVVNKIRGTFKSVAVKA
                     PGFGDRRKAMLQDMAILTGGQVVSETVGLKLDTVGLEVLGTARKIVVTKDETTIVEGS
                     GEAAQIAGRVSQIRAEIENSDSDYDREKL"
BASE COUNT          377 a          749 c          774 g          284 t
ORIGIN      
        1 acgacgagca ggaggacccc ggtggtgtgc gacggcgtcg ggaacggcag cacgcgaccc
       61 aagagcgtcg gctggtagag cgcgggggcg tacccgtgcc cgatcacgtc gttcgtgagg
      121 aacaggacgt cgtagccgag gaacaggtac acggccgcgc gcagcaccgc gacgcgcgcg
      181 agcggtgccg gacgcgtcca ccagccgacg acagcgcgcg cgcccgtggc ggtgcgcccg
      241 ctccaggtcg caggcgcgct catggctgca ccacctgcca ggtcgtcagc gtgtcgagga
      301 cggcgtcgcc gaccgggcgt ccaccctgca gctgccgcgt cgagcgcacg accaggatct
      361 cgacgtaggc gggctcgtcg gggtggagcg ccgcgtgcgc atcggagatc gactgcagca
      421 gcgccgggtc ggcgaggatc cggccgagct ggccctcgat ctccgcgcgt ccgacgccga
      481 cgccggtcgg gttgagcggt accgggaccg tcgcgcccgt cgagtcgacc gcgtcgaggt
      541 gcaccgagcg gaccgtgccg gccgggtcgt gcgcggacgc gtactgcgag agcgagccga
      601 gcgggaacca gtcgttggtg tcgacgagct gggccgacgc gagcaccgcg agtcccgcgc
      661 cggcgactgc gagccgccag gtccgggcgg atcggcccag gacccgcacg ggcgcggccg
      721 gctcaacggg gtgggggagg acggggttcg cgggtgtcgg gctctggtcg ggcgggctcg
      781 gcatggcccg cacactacgg ctgaccgggt gtggacggcc accctgcctc gccatctgcc
      841 aggcgcgaac acgagtcgcc cgatccgcgc gaacatgccg atcaggcccg tgcggagagc
      901 cgtgtgcttg tgcgtcgtct tgcactctcc gggggtgagt gctaaccatg gacttagcac
      961 tctccggtgg agagtgacag aatcaaccgg ctgaccggtg aggttcgcac ccagacggga
     1021 catgaccgtc cgggggcgtg ccgtccgtcg cgggcatcgt cagccgaacc gagccacagt
     1081 gaaggatcac agccccatgg ccaagatcat tgccttcaac gaggaggccc gtcgcggcat
     1141 cgagcgagga ctcaacatcc tcgccgacac cgtcaaggtc accctcggcc cgaagggccg
     1201 caacgtcgtc ctggacaaga agtggggcgc ccccacgatc accaacgacg gcgtctccat
     1261 cgccaaggag atcgacctcg acgacccgtt cgagaagatc ggtgccgagc tcgtcaagga
     1321 ggtcgccaag aagaccgacg acgtcgccgg cgacggcacc accaccgcca cggtcctggc
     1381 ccaggcgctc gtgcgcgagg gtctgcgcaa cgtcgcggcc ggcgccaacc cgatcgccct
     1441 gaagaagggc atcgagaagg ccgtcgaggc cgtcatcgcc cagctcctcc tgcaggccaa
     1501 ggatgtggag accaaggagc agatcgctgc gaccgcctcc atctcggccg gcgacaccgc
     1561 gatcggcgag ctcatcgccg aggcgctcga caaggtcggc aaggaaggcg tcatcacggt
     1621 cgaggagtcc agcgcgctgg gcctcgagct tgagctgacc gagggcatgc gcttcgacaa
     1681 gggctacctg tcggcgtact tcgtcacgga cccggagcgc caggaggccg tcctcgagga
     1741 cgcctacgtc ctgctcgtcg agtccaagat ctcgaacgtc aaggacctgc tgcccctcct
     1801 ggagaaggtc atccaggccg gcaagccgct gttcatcatt gctgaggacg tcgagggcga
     1861 ggccctggcc acgctcgtcg tcaacaagat ccgcggcacg ttcaagtccg tcgccgtcaa
     1921 ggccccgggc ttcggggacc gccgcaaggc gatgctccag gacatggcga tcctcaccgg
     1981 cggccaggtc gtctccgaga ccgtcggtct caagctcgac acggtcggcc tcgaggtcct
     2041 cggcacggcg cgcaagatcg tcgtcaccaa ggacgagacg accatcgtcg agggttcggg
     2101 cgaggcggcg cagatcgccg gccgcgtctc ccagatccgc gccgagatcg agaactccga
     2161 ctcggactac gaccgcgaga agct
//
LOCUS       DASRKH010000187         2463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_3313, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000187 DASRKH010000000
VERSION     DASRKH010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3313"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VFC06_03110"
     CDS             <1..492
                     /locus_tag="VFC06_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80922.1"
                     /translation="RRARVAMPLAMVCFAVPYVAMLFTVQWLQPAGQEGFADLAYFVG
                     LLLLGAVVVAVVIPIVVFSLGRVLDAAALAWGTARAALAFGGAGLALGLVLAALMSWA
                     QDVSMVGATANVALPAAIGGLGTRLLLPVALAHRWVVVLAWVLAALPVAGVVALVLSV
                     RFT"
     gene            606..1526
                     /locus_tag="VFC06_03115"
     CDS             606..1526
                     /locus_tag="VFC06_03115"
                     /inference="COORDINATES: protein motif:HMM:NF013255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZL80923.1"
                     /translation="MNTVTVVAGANGFVGSQICRALLKNGETVRAVVRRAGSMVAHPG
                     LEEWVGDFADPVFATNVCAGARSVVTTVHPMASDFETQRKVGVDGTSDFARAARDAGV
                     ELLIHISTAGVYERESDTGDVKESSRLVSDAAGPYAVTKRDAEASLENVTGLTRVFIR
                     PPAILGRGESSTWNSILPAQIRDDEAARHAVPDRTFAWVHVNDLASFAADIATRRVAT
                     SNDPEVGPVPGSTTAVNVAAPAAKVRDYYVAVTTALGVAPIWDDQPAWTGRLVADRAR
                     AWGWSPTVGLEQAMVELVAPLSGTGRAPSH"
     gene            complement(1537..2211)
                     /locus_tag="VFC06_03120"
     CDS             complement(1537..2211)
                     /locus_tag="VFC06_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HZL80924.1"
                     /translation="MCGRYADFLADQDLADAFSLAITTNDERLLPPSYNVAPMQRVRV
                     IRSADGAATLDVAKWGLVPSWAKDPSIGNRMINARVETIAEKPSFRTAFARRRCIVPA
                     SGYFEWQTDASGKRPFYIHPADGSPLAFAGLVEAWRPTGDDDWLITCAIVTTAARDEM
                     QQIHDRQPVMMRGDAWRTWLDPASAKDELLDAALADAPELAWHEVGKAVGNVRNNVPE
                     LVEPAR"
BASE COUNT          358 a          773 c          870 g          462 t
ORIGIN      
        1 cggcgagcgc gcgtggcgat gcccctcgcc atggtgtgtt tcgcggtgcc ctacgtggcg
       61 atgctcttca cggttcagtg gctccagccg gcgggtcagg aggggttcgc cgacctcgca
      121 tactttgttg gcctgctgct cttgggcgcg gtggtcgtgg cggtcgtcat ccccatcgtg
      181 gtttttagcc tgggcagggt gctcgatgca gcagcgctcg catgggggac ggcgcgcgcg
      241 gccctggctt tcggcggcgc cggcctagct ttggggctcg tgttggcggc gctcatgtcg
      301 tgggcgcagg acgtttccat ggtgggcgca accgccaatg tggcgttgcc cgcggcgatc
      361 gggggacttg gcacacgcct cttgcttccc gtggcgttgg cacatcgctg ggtcgtggtg
      421 ctcgcctggg tcctcgccgc tttgccggtg gcgggggtcg tggcgctcgt tctctccgtg
      481 cgtttcacgt aactcgctag ccggcgggct ccaccagctc gggagagttg ttgcgcactt
      541 tgcccatacg ggcccgtgcg tgagaccgcg ccccgactgg tgtcgaggcc tgcgatgatg
      601 ctgaggtgaa cactgtcact gttgtcgcgg gtgcgaatgg ttttgtgggc tcgcagatct
      661 gccgcgcgct gctcaaaaac ggcgagaccg ttcgagccgt ggtgcgtcga gcaggatcta
      721 tggtggcgca tccggggctc gaggagtggg tcggcgactt cgctgacccc gtgttcgcga
      781 ccaacgtgtg tgctggtgcg cgttcagtgg tgacaacggt gcacccgatg gcgagcgact
      841 tcgagaccca gcgcaaggtg ggggtcgacg gtacgtctga cttcgcccgc gccgcgagag
      901 acgccggtgt cgagctcttg atccacatct cgaccgcggg tgtgtacgaa cgtgagtccg
      961 acacagggga tgtgaaggag tcttcgcggt tggtctctga tgccgcagga ccgtacgcgg
     1021 tgaccaagcg tgacgccgag gcttctctcg agaatgtcac tgggctcacc cgcgtgttta
     1081 tccgtccgcc ggcaatcctg ggaaggggcg agtcatcgac ctggaattcg atactgcccg
     1141 cccagattcg cgacgatgag gcggcacgtc atgccgtgcc ggatcggacc tttgcatggg
     1201 tgcacgtcaa cgacctggcc tccttcgccg ccgacatcgc gaccaggcgc gtggcgacgt
     1261 cgaacgatcc ggaggtcggg cctgtgccag gctcgaccac cgctgtcaac gtggccgcgc
     1321 cagccgcaaa ggtgcgcgac tactacgttg cggtgactac ggcattgggc gtcgctccga
     1381 tctgggatga tcagcccgcc tggaccggac gattggtcgc ggaccgcgcg agggcctggg
     1441 gctggagccc gacggttggg ctcgaacagg caatggttga gttggtggcg ccgctgagcg
     1501 ggaccggacg ggcccccagc cactagccga gtccgtctac cgggctggct ccaccagttc
     1561 aggcacgttg ttgcgcacgt tgcccacggc cttgcccacc tcgtgccacg ccagctccgg
     1621 ggcgtcggcc agggccgcgt ccagcaactc gtccttcgcc gacgccgggt ccagccaggt
     1681 gcgccaggca tcgccccgca tcatcacggg ttggcggtcg tggatctgct gcatctcgtc
     1741 gcgcgcggcg gtggtgacga tcgcgcacgt gatgagccag tcgtcgtccc cggtgggtcg
     1801 ccaggcctcg accaggcccg cgaaggccag gggcgagcca tcggcgggat gaatatagaa
     1861 cggccgcttg ccagacgcgt cggtctgcca ttcgaagtag ccgctcgccg ggacgatgca
     1921 gcggcgccgg gcaaaggcgg tgcgaaacga cggcttctcg gcgatcgtct cgacccgcgc
     1981 gttgatcatg cgattgccga tggaggggtc cttggcccac gagggcacca gaccccactt
     2041 cgcgacatcg agcgtcgcgg cgccgtcggc ggagcgaatg acccgcaccc gttgcatggg
     2101 ggcgacgttg tatgaggggg gcagcagacg ctcgtcattt gtggtgattg ccaacgaaaa
     2161 cgcgtcggct aaatcttggt ccgcaaggaa gtccgcgtag cgcccgcaca tgcctctatt
     2221 gtgcctccct gtgacaaagt gcaccctttg acaagccggg gccgtgcggt cgctcgcgtg
     2281 ccccccacgg tgcggcattg gtcacacgac gcgcagggca ctatgacccg ctttgcacca
     2341 catacggggg tgtaaaaggg acaaagtggc cccgttgtga gcatcctcac acgagccccg
     2401 cacgggcccg gtaggcactg caagcgcttg gtaatggggg tccccggtgc catcgttgag
     2461 tca
//
LOCUS       DASRKH010000188         4375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_657, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000188 DASRKH010000000
VERSION     DASRKH010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..1801)
                     /locus_tag="VFC06_03125"
     CDS             complement(68..1801)
                     /locus_tag="VFC06_03125"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017884992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HZL80925.1"
                     /translation="MTDSLRVDGGRPLVGEIHVRGAKNFVSKAMVAALLGSTPSVLRN
                     VPEIRDVNVVSSLLRLHGVDVDYDVVTGVLTMDTSTLTPADAAQIEAHAGSSRIPILL
                     CGPLLHRLGEAVIPDLGGCRIGDRPIDFHLEILTKFGATVAQGEDGLHLTAPHGLRGM
                     RYELEFPSVGATEQLLLTAVLAEGVTTLHNAAVEPEIKDLIDTLQKMGAIISVDTDRS
                     ITIEGVEHLKGYDHSSLNDRIEVGSWAAAALATRGDILVRGAQQRHMGMFLNVFRKVG
                     GEFDVTTEGIRFFHPGTDLKPIALQTDVHPGFMTDWQQPLVVALTQAKGLSIVHETVY
                     ENRFGFTDALVKMGAIISVDTDRSITIEGVEHLKGYDHSSLNDRIEVGSWAAAALATR
                     GDILVRGAQQRHMGMFLNVFRKVGGEFDVTTEGIRFFHPGTDLKPIALQTDVHPGFMT
                     DWQQPLVVALTQAKGLSIVHETVYEHRFGFTDALIQMGAQVQLYKECLGDMKCRFGQR
                     NFRHSAVISGPTPLKAANIEVPDLRGGFSHLIAALAAEGTSTVSGIGIIDRGYENFRA
                     KLAALGASVEA"
     gene            1886..2782
                     /locus_tag="VFC06_03130"
     CDS             1886..2782
                     /locus_tag="VFC06_03130"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HZL80926.1"
                     /translation="MTPTDRPRVLCVGLATLDVIQLVEGLPGSNEKVVALDSLTAAGG
                     PAANAAVAAAHLDGDVVLVTALAEHPVSDIILFDLAACGVTVRTVSVDSPPVVASILV
                     SAGTGDRAIISPTASASGAQTPSLPEAEIAELLEGVTAVHLDGYYPSLAIPIASAARE
                     RRIPVLLDAGSHKPHTDAVLEHVDVAVVSGDFAPRGMVPDPVSVLAYLVERGPTFAAV
                     TRGARGIVFRGPQSRGAVPVERVNVVDTLGAGDFFHGALAVAVAQAGASDQEFSHQLA
                     WASRVAGASLGSFGTRRWLSDS"
     gene            complement(2818..3477)
                     /locus_tag="VFC06_03135"
     CDS             complement(2818..3477)
                     /locus_tag="VFC06_03135"
                     /inference="COORDINATES: protein motif:HMM:NF013424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HZL80927.1"
                     /translation="MKRLSRIPQHIGIIPDGNRRWAAHKGLKKQDGYEFGLQPGLELH
                     EMCLELGVKELTVYGFTVDNTKRPSLQIRAFQKACVDAVEYLATKDADLLVVGNTQSR
                     FFPKALLPYTERTRFGRGRIKVNLLANYGWKWDLDYLVQNSAGHSSGNIIDGIASSGV
                     SRIDLIIRWGGGRRLSGFLPIQSVYSDFYVVDELWPDYRSEQFYEALGWYQDQDVTLG
                     G"
     gene            complement(3614..>4375)
                     /locus_tag="VFC06_03140"
     CDS             complement(3614..>4375)
                     /locus_tag="VFC06_03140"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HZL80928.1"
                     /translation="LVCGSAILLFAVHVRPWGYAPLVAGVVLAWTVDRALARHLALIA
                     FGQVIISTISLKADLSDAGIARFVVVLGLAVLLPFAISRWAWGERIVRFPMRGGWPWT
                     RAQWAYIGVVILAGYLILPYYFIGSGAYRNWPDVSGPNEIARLFVGVNAVGIWDELFF
                     VATIFTLLQRHFPVWLANTLQAAVFVSFLWELGYREWGPVLTVPFALIQGWIFSWARS
                     LPYVITVHLLFDAVVFGVLVHAHRPELLDIFVTAP"
BASE COUNT          763 a         1453 c         1409 g          750 t
ORIGIN      
        1 acgcaggccg ccatccggta cagcctgccg cggggctgcc gcagaggaat cctcttgacc
       61 atggtcccta cgcctcgacg ctcgcgccca gtgccgccaa cttggcgcgg aagttttcgt
      121 agccacgatc gatgatgccg atgccggaca cggtggacgt tccttcggcc gcgagcgcgg
      181 cgatcaggtg ggagaagccg ccgcgcaggt ccggcacctc gatattggcg gccttgagcg
      241 gcgtggggcc actgatgacc gcggagtgcc ggaaattccg ctggccgaag cggcacttca
      301 tgtcgcccag gcattccttg tacaactgca cctgcgcgcc catctggatc aaggcgtcgg
      361 tgaagccaaa gcggtgctcg tacacggtct cgtgcacgat gctgaggccc ttggcctggg
      421 tcagcgccac cacgagcggc tgctgccagt cggtcatgaa gccggggtgg acgtcggtct
      481 gcagcgcaat cggcttgagg tcggttccgg ggtggaagaa gcgaattcct tcggtcgtga
      541 cgtcgaattc gccgcccacc ttgcggaaca cgttcaagaa catgcccatg tgacgctgct
      601 gcgctccccg caccaggatg tcgccacggg tcgcgagcgc ggcggcggcc caggatccga
      661 cctcgatgcg gtcgttgagc gacgaatggt cgtagccctt caagtgctcg acgccctcga
      721 tcgtgatgga acggtccgtg tcgaccgaga tgatcgcgcc catcttgacg agcgcgtcgg
      781 taaaaccaaa gcggttctcg tacacggtct cgtgcacgat gctgaggccc ttggcctggg
      841 tcagcgccac cacgaggggt tgctgccagt ccgtcatgaa gccggggtgg acgtcggtct
      901 gcagcgcaat cggcttgagg tcggttccgg ggtggaagaa gcggattcct tcggtcgtga
      961 cgtcgaattc gccgcccacc ttgcgaaaca cgttcaagaa catgcccatg tgacgctgct
     1021 gcgctccccg caccaggatg tcgccacggg tcgcgagcgc ggcggcggcc caggatccga
     1081 cctcgatgcg gtcgttgagc gacgaatggt cgtagccctt caaatgctcg acgccctcga
     1141 tcgtgatgga acggtctgtg tccaccgaga tgatcgcgcc catcttctgg agcgtgtcaa
     1201 tgaggtcctt gatttctggc tcgaccgccg cgttatgcag cgtcgtgaca ccctcggcca
     1261 gcaccgccgt cagcaacaac tgctcggtgg cgcccaccga cgggaattcg agttcgtacc
     1321 tcatgccgcg caagccatgc ggcgcggtca ggtggagccc gtcctcacct tgggcgaccg
     1381 ttgcgccgaa cttggtgagg atttccaggt ggaagtcgat gggccggtcg ccgatgcggc
     1441 agccgcccag gtcagggatg accgcctcac ccagccggtg aagcagcggc ccacacagca
     1501 ggatcgggat gcgcgacgag ccggcgtgcg cctcgatctg cgcagcatcc gcgggggtca
     1561 gggtcgacgt gtccatggtg agcacgccgg tcaccacgtc gtagtcgacg tccacgccgt
     1621 gcagcctcag cagcgacgac accacattca cgtcgcgaat ctcggggacg ttgcgcagca
     1681 cggagggcgt ggagcccaac aacgcggcca ccatcgcctt ggatacaaag ttcttggcac
     1741 cgcgtacgtg aatttcgccc accaggggcc gaccgccatc gacgcgcaga gagtccgtca
     1801 tggtcactat cgtgccccat gtttgctccg tcccgcgcct cctccgctcg gaccctcgac
     1861 gtgcccctcg tcctagactc gtcgcgtgac acccactgac cggccgcgag tcctgtgcgt
     1921 cggcctcgcg acgctcgacg tcattcagct ggtggagggc ctgccgggca gcaacgagaa
     1981 ggtcgtggcc ctggactcgc tcaccgccgc gggcggaccg gccgcgaatg ccgcggtcgc
     2041 ggcagcccat cttgacggcg acgtcgtgct cgtcaccgcg ctggcagagc acccagtgag
     2101 cgacatcatc ctttttgacc tcgccgcgtg cggcgtgacg gtccgcacgg tttcggttga
     2161 ctcgcctccc gtcgtggcgt cgatcctggt gtccgccggc accggcgacc gtgcgatcat
     2221 ctcgcccact gcgtcggcct cgggggcgca gaccccgtca cttccggaag ccgagatcgc
     2281 cgaactgctt gagggcgtga ccgcggtgca cctcgacggc tactacccgt cgctcgccat
     2341 tccgatcgcg tccgcggccc gcgaacgccg catcccggtc ctgctcgacg cgggcagcca
     2401 caagccccat acggacgcgg tgcttgagca cgtcgacgtc gccgtcgtgt ccggagactt
     2461 cgcgccgcga ggaatggtgc cggaccctgt gagcgtgctg gcgtacctgg tcgaacgcgg
     2521 gccgacgttt gccgccgtca cgcgaggggc gcggggcatc gtcttccgcg ggcctcagag
     2581 ccgcggcgcg gtgccggtcg agcgtgtcaa cgtcgttgac accctgggtg ccggcgactt
     2641 cttccacggc gctctcgccg ttgccgtcgc tcaggcgggc gcgtcggatc aggagttctc
     2701 ccaccagctc gcgtgggcga gccgcgtcgc aggtgcatcg ctcggcagct tcggcacccg
     2761 gcggtggctc agcgactcct agcctcacgc cttcacggag ccgccgatgg cgaccggcta
     2821 accgccgagc gtgacgtctt ggtcctgata ccagcccaag gcctcataga actgctcgct
     2881 gcggtagtcg ggccacagct cgtccaccac gtagaagtcc gaatacaccg actggatcgg
     2941 caggaatccg ctgagcctgc gcccgcctcc ccagcggatg atcaagtcga ttcgtgagac
     3001 gccagatgag gcgatgccgt cgatgatgtt gccgcttgaa tggccggcac tgttctgcac
     3061 caaatagtcg agatcccact tccagccgta gttggccagg aggttgacct tgatgcggcc
     3121 gcgcccgaat cgggtcctct cggtataggg cagcagcgcc ttggggaaga atcgcgactg
     3181 ggtgttgccg acaaccagaa gatccgcatc tttggttgcc aggtattcca cggcgtccac
     3241 gcacgccttt tgaaatgccc ggatctgcag ggaggggcgt ttggtgttgt cgaccgtgaa
     3301 gccgtagacg gtgagctcct tcacgcccag ctccagacac atttcgtgca actcgagtcc
     3361 tggctgaagg ccgaactcat acccgtcctg cttcttcaat cccttgtgcg cggcccaccg
     3421 tcgattgccg tccggaatga tgccaatgtg ttgcgggatt cgactcaggc gtttcatcac
     3481 tgcctgctgc gtactgtagc gacgggcgtg ccgaatcgtt gcgtcatggg ctcaatcctg
     3541 ccacgcgaga tcttcaaggc tagccaaagt gctcgtcagt gttgagcaat tgattcaccg
     3601 agccgttgag cggctagggt gcggtcacaa agatgtcgag cagttctggg cgatgggcat
     3661 gcaccagcac gccgaacacc acagcatcga acagcaggtg caccgtgatg acgtacggca
     3721 gcgaccgcgc ccacgagaag atccacccct ggatgagcgc gaacggcacc gtgagcaccg
     3781 ggccccactc tcggtaaccc agctcccaca ggaacgagac gaagacggcg gcctggaggg
     3841 tgttggccag ccacacggga aaatgccgtt gtagcagggt gaaaatcgtg gccacgaaga
     3901 agagttcgtc ccagatgccg acggcgttga cgccgacaaa gagccgggca atttcgttcg
     3961 gcccgctcac atccggccag ttccggtacg cccccgagcc gatgaagtag tacggcagga
     4021 ttagataccc ggcaaggatc acaaccccga tgtacgccca ctgggctcgc gtccatggcc
     4081 agcctccgcg catcgggaag cggacgatgc gctcgcccca cgcccaccgc gagatcgcga
     4141 acggcagcag gaccgctagc cccaagacga ccacaaacct cgcgatcccc gcgtcggaga
     4201 ggtcggcctt gagcgagatg gtcgagatga tgacctgacc gaacgcgatc agcgcgaggt
     4261 gtcgcgcgag cgcgcggtcg acggtccagg cgagcacgac gccggcgacc agcggcgcgt
     4321 agccccacgg ccgcacgtgg accgcgaaca gcaagattgc cgagccacat acgag
//
LOCUS       DASRKH010000189         3068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_44046, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000189 DASRKH010000000
VERSION     DASRKH010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44046"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="VFC06_03145"
     CDS             <1..693
                     /locus_tag="VFC06_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019618730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase (NADP(+))"
                     /protein_id="HZL80929.1"
                     /translation="GFLFAPAGAALSEGIATRASAIAEHRLLNPGITSGYRSRRDAVL
                     AAPGVTVLAEGDLRVDDNGQGWATPTIVSVPVSVLKEHREELLDESFGPLSIVVEYDS
                     VEDLANVAEDLFEGNLTGTVHAAAGENSPALRSLVDWISRSAGRVLFGGWPTGVAVTP
                     AMQHGGPWPATTNDSGTSVGTAAIARFTRPVAYQSVPQELLPEPLRDDNPWGVPQHRS
                     EAGQSASWGNLA"
     gene            complement(969..2234)
                     /locus_tag="VFC06_03150"
     CDS             complement(969..2234)
                     /locus_tag="VFC06_03150"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HZL80930.1"
                     /translation="MTSMTSLTPAAKPERLPGSLFGGIDTHKNTHHVAVVDGVGRPVA
                     DREFATTPTGYTEIVALFHSHGAVEQVGVEGTGSYGAGISRVLSAAEFTVMEIARPNR
                     RHRRLKGKSDPIDAHQAALAVIAGTEAAIPKRGDGEVESLRILMSERHSAVKARSQAM
                     NQIHGLLITAPDQVRQDYRALGGEKLVDVLSRSRPAPGADPTMVARHTLRRLAIRHTL
                     LRDEITVINNQLDTLIRILNPGLLALSGVGVVTAATLLTTAGDNPERLASAAAFAALC
                     GAAPIPASSGQRVRHRLSRGGNRKANSALHRIVLLRMRHQEPRTAAYFARRRAEGLSD
                     RDIVRCLKRHIANEVFRTLISPIPATPAGDLLRQRRQALGIPITTLAATLEIPYQRLR
                     RLEIGTRGDTKLEQQAHHALDQISSRKAA"
     gene            complement(2477..>3068)
                     /locus_tag="VFC06_03155"
     CDS             complement(2477..>3068)
                     /locus_tag="VFC06_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZL80931.1"
                     /translation="LTARGLTGVQLVTSDAHSGLKDAIAANLPGAAWQRCRTHYAANL
                     MSVTPKSMWPAVKAMLHSVYDQPTAEDVHSQFDRTLDYVTEKLPAVAEHLEGARADIL
                     AFTTFPKDIWAQIWSNNPNERLNREIRRRTDSVGIFPNRDSVIRLVGAVLAEQTDEWT
                     ESKRYLGLEVLTRCRLTPVPDTDAEEVDAGPLALTA"
BASE COUNT          481 a          902 c         1090 g          595 t
ORIGIN      
        1 ggtttcctgt tcgctcctgc gggcgcagcg ctctcggagg ggatcgcgac ccgcgcgtcc
       61 gctattgcgg agcatcggct tctgaacccg ggaatcacct ccggctatcg ttctcggcgc
      121 gacgccgttc tcgccgctcc cggtgtgacg gtgctcgctg agggcgacct gcgcgtcgac
      181 gataacggcc aagggtgggc gactcccacc attgtctcgg ttccggtgag tgtgctcaag
      241 gagcatcgcg aggagttact tgacgagtct ttcggtccgc tttccatcgt ggtcgagtac
      301 gacagtgtgg aggaccttgc gaatgtcgcc gaggatctct tcgagggcaa cctgactggc
      361 acggtgcacg ccgccgccgg cgagaactcg cccgcgctgc gaagcctcgt cgactggatt
      421 tcccgcagtg ctggtcgggt gttgttcggc ggttggccga ccggtgtggc cgtcaccccc
      481 gccatgcagc acggaggccc gtggcccgca acaaccaacg actcgggcac ctcggtcggt
      541 accgcggcga tcgcccgctt cacgcgtccg gtggcgtacc agagtgtgcc gcaggagttg
      601 ctaccggagc cgctgcgcga tgacaacccg tggggtgtac cgcagcatcg ttcagaagcg
      661 gggcagtcgg cttcatgggg gaacttggcg taatacggcg ttgcagagcg cgcggaaggc
      721 cgggggagcg agtctgaacg agacggtgat gccccggctc cgacgtgacg atcagagggc
      781 cctagtccgg gctccacctc accgctcacg gtctcgagtc agtcccggga atcttggggc
      841 gggagcgcat cgtcagcgcc gtcgccttcc gcgacggcca cgatccggtt caccatgccg
      901 ctgaagatca gaccgtggaa cggcagcatg gacggtcaag tcccgggaag tggatgctcc
      961 tattggtgtc aagccgcttt tcgggagctg atttggtcga gcgcgtggtg tgcttgctgt
     1021 tcgagtttgg tgtcgccgcg ggtcccgatt tcgagtcggc gtagccgctg gtaggggatt
     1081 tcgagggtgg cggcgagggt cgttatcggg atgccgagcg cttgtcgccg ttggcggagc
     1141 aggtcaccgg ctggtgtcgc ggggatcggg ctgatgaggg tgcggaagac ctcgttggcg
     1201 atgtgtcgct tcaggcagcg gacgatgtcc cggtcggaga gcccttcggc gcgacggcga
     1261 gcgaagtagg ctgcggtgcg cggttcctgg tgtctcatcc gcaggagcac gatccggtgc
     1321 agcgcactgt tcgctttccg gttccctcca cgggacaggc ggtggcggac tcgttgtccg
     1381 gacgacgcgg ggatcggggc ggctccgcag agcgcggcga acgctgccgc ggaggctagc
     1441 ctttcggggt tgtcgcccgc cgtggtcaga agggtggctg cggtgacgac gccgacgccg
     1501 ctgagcgcga gcaggcctgg gttaagaatc cggatgaggg tgtcgagttg gttgttgatc
     1561 accgtaatct cgtctcgaag cagcgtgtgc cggatggcga gacgtctcag ggtgtgcctg
     1621 gcgaccattg tgggatcagc tccaggagct ggccggcttc ggctgagaac atccacgagc
     1681 ttctcgccgc ccagggcccg gtagtcctgg cgaacctggt cgggtgccgt gatcaggagt
     1741 ccgtggatct ggttcatggc ctgcgatcgg gctttgaccg cagaatgccg ctcgctcatc
     1801 agtatccgca gcgattcgac ctctccatcg cctcgtttgg gaatcgcggc ctcggtgccg
     1861 gcgatgacgg ccagagccgc ctggtgcgca tcgatcgggt ctgacttgcc tttgagcctg
     1921 cggtgccgcc gattcggccg cgcgatctcc atcacggtga actccgccgc tgacaggacg
     1981 cgggagatac ccgctccgta ggaacccgtc ccctcgacac cgacttgttc gacggccccg
     2041 tgcgagtgga acaacgcgac gatctcagtg taaccggttg gcgtagtcgc gaactcgcga
     2101 tccgcgacgg gccggccgac cccgtcaacc acagcaacgt gatgggtgtt cttgtgggtg
     2161 tcgatcccgc caaacaatga ccccggcaag cgctcgggct tggctgctgg cgtgagtgat
     2221 gtcatgctcg tcatggacga gttcctttcc gttgatgagt gaacagtcat cgcgggctgg
     2281 gtggggcaga caacacaaag gtgagacgac ttcccggtca ggctcctatc aagtcatgtc
     2341 ctgcccggcc acgcgacagt ggcccggcag cgtggacaaa tccacataaa gacaacccgt
     2401 gaagggtgtc ggtgtaacaa gcgagtcaca cgccgccgga cacgcatcca tcatctctgt
     2461 gtagcgggtt ccttcgttag gcggtgaggg cgaggggccc ggcgtccacc tcctcggcgt
     2521 cggtgtcagg gacgggggtg aggcggcagc gggtgaggac ttcgagtccg aggtagcgct
     2581 tggactcggt ccattcgtcg gtttgctcgg ctaacacggc tccgacgaga cggatgacgc
     2641 tgtcgcggtt ggggaagatc cccaccgaat ctgtgcggcg acggatctca cggttgagac
     2701 gctcgttggg attgttcgac cagatctgtg cccagatgtc tttggggaaa gtggtgaagg
     2761 cgaggatgtc agcccgggcg ccctcgaggt gttctgcgac cgcggggagt ttctcggtga
     2821 cgtagtccag ggtgcggtcg aactgggagt ggacgtcttc ggcagtgggt tggtcataga
     2881 cggagtgcag cattgctttc acggccggcc acatgctctt cggcgtgaca gacatcaggt
     2941 tcgcggcgta atgggtgcgg caacgctgcc aggcagctcc cggtagattc gctgcgatcg
     3001 cgtccttcag cccgctatgg gcgtcggagg tgaccaactg aacaccggtc agaccgcggg
     3061 cggtcagg
//
LOCUS       DASRKH010000190         2589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_29227, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000190 DASRKH010000000
VERSION     DASRKH010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29227"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VFC06_03160"
     CDS             <1..543
                     /locus_tag="VFC06_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80932.1"
                     /translation="RIAGDQPVQRYEADPVAAPHWYANGNNIVWSFVVPVGLLLAGPV
                     AYGLSAIARRLRRRPRLPRTMAPGVGRWLAASAFAVVGTWVVFVAYLRQVADYAFNYR
                     TNPAFTFGVYGVEWTLALATAFLLAGTVIRVSQQRRTGAVLSVPAAITTGGTVTGVFI
                     LLVLAAYWSGFPDAFGGFGS"
     gene            750..2465
                     /gene="leuA"
                     /locus_tag="VFC06_03165"
     CDS             750..2465
                     /gene="leuA"
                     /locus_tag="VFC06_03165"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HZL80933.1"
                     /translation="MPLAKYRSFHELNTVSLPDRQWPSRTITKAPRWLSTDLRDGNQA
                     LIEPMNPARKRMMFDLLVSMGYKEIEVGFPSASQTDFDFVRALLEDGAIPEDVTISVL
                     TQSREELIHRTIDAVLGIPRASVHLYNATAPVFREVVFRNDRAATLDLAVTGTRDVMA
                     YAEKVLPDDVVFGYEYSPEVFVDTELDFALEVCEAVMDTWEVGPGREIILNLPATVER
                     CTPNVYADQIEWMSRHLSRREHVCVSVHPHNDRGTAVASAELAVMAGADRVEGCLFGQ
                     GERTGNVDLITLALNLFSQGIDPMIDLSDIDEVRRTVEFCTQMDVPARQPYGGDLVYT
                     AFSGSHQDAIKKGFEARQKAAADAGVGVDALIWEMPYLPVDPKDVGRSYEAVIRVNSQ
                     SGKGGIAYLLSSTRNLELPRRLQIELAGIVQKHTDTFGGEISAEKLWEIFADEYLPAS
                     AAEGLTPWGRFSMRGTTVTTADEGKRAVLTVTVVDHGEEKVLAATGNGPIDAFTEALR
                     HVGVDVKVLDYVEHALSAGGDAYAAAYVEAEVDGQVLWGVGIDPSITTASFKAIVSAV
                     NRALR"
BASE COUNT          383 a          914 c          894 g          398 t
ORIGIN      
        1 cgcatcgccg gcgaccagcc ggtgcagcgc tacgaggcgg accccgtcgc cgcgccccac
       61 tggtacgcga acgggaacaa catcgtctgg tccttcgtgg tgccggtggg actcttgctg
      121 gcgggcccgg tggcgtacgg gttgagtgcg atcgcgcgcc ggctgcgccg tcggccccgg
      181 ctcccgcgga ccatggcgcc gggggtgggt cgctggttgg cggccagcgc gttcgcggtg
      241 gtgggcacgt gggtggtgtt cgtggcgtac ctccgccagg tggctgacta cgccttcaac
      301 taccggacga acccggcctt caccttcggg gtgtacggcg tggagtggac actcgccctc
      361 gccaccgcat tcctccttgc cggcaccgtg atccgcgtct cccagcagcg ccgcacgggc
      421 gctgtcctgt ccgtcccggc agcaatcacc accggtggca ccgtgacggg cgtgttcatc
      481 ctgctcgtgc tcgcggccta ctggagcggg ttcccggacg ccttcggcgg tttcggcagc
      541 tgagcatcca gcggcccgga cgtgactcca cgcccgatcg ggggtaacct gcggagatgc
      601 acgcggttgc actgctcctt ccatgccgcg gcggggcccc atcgggccgg cccctcgccg
      661 cggtgtgatt cacgccggcc gagaggctca aacccaagga aacagcagtg aaaacccagt
      721 cccacatccg cgcccagcag ccctccggca tgccgttggc gaagtaccgg tccttccatg
      781 aactgaacac cgtctcgctc ccggaccgtc agtggccgtc ccggacgatc acgaaggccc
      841 cccgctggct ctccaccgac ctgcgggacg gcaaccaggc cctcatcgag ccgatgaacc
      901 cggcgcgcaa gcgcatgatg ttcgacctgc tcgtctcgat gggctacaag gagatcgagg
      961 tgggcttccc gtcggcgtcc cagacggact tcgatttcgt ccgcgccctc ttggaggacg
     1021 gcgcgatccc ggaggacgtc accatctccg tgctgacgca gtcgcgggag gagctgatcc
     1081 accgcaccat cgatgcggtc ctgggcatcc cgcgcgcgag cgtgcacctc tacaacgcga
     1141 ccgccccggt gttccgcgag gtggtgttcc gcaacgaccg cgccgcgacc ctggacctcg
     1201 ccgtcaccgg gacgcgggac gtgatggcgt acgcggagaa ggtcctgccg gacgacgtgg
     1261 tgttcggcta cgagtactcc ccggaggtct tcgtggacac cgagctcgat ttcgcgctcg
     1321 aggtgtgcga ggcggtcatg gacacgtggg aggtgggccc gggccgggag atcatcctga
     1381 acctgcccgc caccgtggag cgctgcaccc ccaacgtgta cgcggaccag atcgagtgga
     1441 tgagccgcca cctgtcccgg cgggagcacg tctgcgtctc cgtccacccg cacaacgacc
     1501 gcggcaccgc cgtcgcctcc gcggaactgg ccgtcatggc cggcgcggac cgcgtcgagg
     1561 gctgcctgtt cggacagggc gaacgcacgg gcaacgtcga cctcatcacc ctggcgctga
     1621 acctgttcag ccagggcatc gacccgatga tcgacctgag cgacatcgac gaggtgcggc
     1681 gaacggtcga gttctgcacc cagatggacg tcccggcccg gcagccgtac ggcggggacc
     1741 tggtgtacac ggccttctcc ggctcgcacc aggacgccat caagaagggc ttcgaggctc
     1801 ggcagaaggc cgcggccgac gccggggtcg gggtcgacgc gttgatctgg gagatgccgt
     1861 acctgccggt cgacccgaag gacgtcgggc gcagctacga ggccgtcatc cgggtcaact
     1921 cgcagtccgg caagggcggc atcgcctacc tgctgtcctc cacgcggaac ctggagctgc
     1981 cgcgccgcct ccagatcgag ctggccggga tcgtgcagaa gcacacggat actttcggcg
     2041 gcgagatcag cgcggagaag ctctgggaga tcttcgccga cgagtacctg ccggcctcgg
     2101 ccgcggaggg cctcacgccg tggggacgct tctccatgcg tggcacgacc gtgacgacgg
     2161 cggacgaggg caagcgcgcc gtcctcaccg tcaccgtggt tgaccatggt gaggagaagg
     2221 tgctcgcggc caccggcaac ggccccatcg acgccttcac ggaagcgctc aggcatgtcg
     2281 gcgtggatgt caaggtgctc gactacgtgg agcacgcgct gtccgcgggc ggggacgcgt
     2341 acgcggccgc ctacgtggag gccgaggtgg acggccaggt gctgtggggc gtcgggatcg
     2401 acccgtccat caccaccgcc tcgttcaagg cgatcgtctc cgcggtcaac cgcgccctcc
     2461 ggtagcgggc agctgggctc gttgtcgggc cggtggggcc gccgtaccct gaaggggtgt
     2521 ccgacggcct cgccagcccg cccgccttcc cgctcgtcga cgacgtcgcc gcgcgggcgc
     2581 tccacctcg
//
LOCUS       DASRKH010000191         2203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_17369, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000191 DASRKH010000000
VERSION     DASRKH010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17369"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..254)
                     /locus_tag="VFC06_03170"
                     /pseudo
     CDS             complement(1..254)
                     /locus_tag="VFC06_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013115419.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
     gene            159..674
                     /locus_tag="VFC06_03175"
     CDS             159..674
                     /locus_tag="VFC06_03175"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HZL80934.1"
                     /translation="MRGVGTAQLLAPRGRHRLEVAARVPRRQPVHTTQSELLAQRTGV
                     CQDFAHLMIAALRVHGLPARYASGYLETMPPPGREKLRGADASHAWVSAWVPGAGWVE
                     IDPTNDKFIDDRYVVLGWGRDYSDVPPLRGVIFTEGPGAKPSVSVDLVPTAAPVSPVD
                     TVPANSEGPSE"
     gene            complement(1427..1972)
                     /locus_tag="VFC06_03180"
     CDS             complement(1427..1972)
                     /locus_tag="VFC06_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015884067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZL80935.1"
                     /translation="MTSDSHETPPAIEPDTKDWTWVLDERCPECGYAAPDVDPTAIGT
                     TVRALAPRWVGALSRRDARDRPAPATWSVLEYSAHVRDVHLVFGGRLASMLEHDDPLF
                     DSWDQDAAALDDRYDLQAPAVVADELADAADALADAFDAVTAEEWDRPGRRSNGSVFT
                     VRSLGLYFLHDVVHHLHDVGA"
BASE COUNT          280 a          835 c          750 g          338 t
ORIGIN      
        1 gcgccggggc cctcggtgaa gatgacgccc cgcagcggcg gcacgtcgga gtagtcacga
       61 ccccagccga ggacgacgta ccggctgtcg atgaacttgt cgttcgtcgg gtcgatctcg
      121 acccagccgg accccggcac ccaggcggac acccaggcgt gcgaggcgtc ggcaccgcgc
      181 agcttctcgc gccccggggg cggcatcgtc tcgaggtagc cgctcgcgta ccgcgccggc
      241 agcccgtcca cacgacccag tccgagctgc tcgcgcagcg caccggggtc tgccaggact
      301 tcgcgcacct catgatcgcg gcgctgcgtg tgcacgggct gccggcgcgg tacgcgagcg
      361 gctacctcga gacgatgccg ccacccgggc gcgagaagct gcgcggcgcc gacgcctcgc
      421 acgcctgggt ctccgcctgg gtgcccggag ccgggtgggt cgagatcgac ccgacgaacg
      481 acaagttcat cgacgaccgg tacgtcgtcc tcggctgggg tcgcgactac tcggacgtgc
      541 cgccgctgcg gggcgtcatc ttcaccgagg gccccggcgc caagccgtcg gtgtcggtcg
      601 acctcgtgcc caccgctgct ccggtctccc cggtcgacac cgtccccgcc aacagcgaag
      661 gcccctcgga gtaagcgtcc gaggggcctt gctgggccgc gggtgccccg ccggcgaacc
      721 ggcggtgctt cgtcgcggat gactctgcgg ctccgtctcg gcgatgatcc tccggttgcc
      781 cggtctctcg gtgtcgagac gtcctgcggt cggtctcccg gtccctgcgg tcctgctgcg
      841 tcgtcatccg cagctcctcg ggttgccccg cgaagacggt ccgccgttct ccgttcgacc
      901 acggcctcca ggtccccgtc atcctcggtc agtgtccccc ttgcgggagt ccttaccggt
      961 ggatctcggt ccttcgaggt cgttggctca ggtctagtcc tcccgcaccg ggcccgcaag
     1021 gggtaccacc aactttcttt ttcccccggt cggtgcacat gttcatccac aggttggcct
     1081 gcataaacgc gccatcagca cagccctgtg gacaacatct gtggacgaca cgcgggggac
     1141 gccacgccgg tcgctcttga gggggcgacc gctcgtccgc ccccgagttc gtcaccggaa
     1201 gttttcccga tcgccgcgcg tcgctcaccg ggctccgaca gtgcctcccg tagcctggaa
     1261 ctcagcgaag gcccctcgga gtaagcgtcc gaggggcctt gctgtgtctc cgagggccgt
     1321 tcaggcccgg ttcgagcgcc tcacacgccc gcaggcatgg gacctgtgga cacatctgtg
     1381 gacaggggtt gtggacaaga cccctgcgaa gtcgtgatcg agaggctcag gcgcccacgt
     1441 cgtggaggtg gtgcaccacg tcgtgcagga agtacaggcc gagcgagcgg accgtgaaga
     1501 ccgacccgtt cgaccgtcgt ccgggcctgt cccactcctc cgccgtcacg gcgtcgaacg
     1561 cgtcggccag cgcatcggcc gcgtcggcga gctcgtccgc gaccaccgcc ggggcctgca
     1621 ggtcgtagcg gtcgtccagg gccgccgcgt cctggtccca gctgtcgaac agcgggtcgt
     1681 cgtgctcgag catgctcgcg agccggcccc cgaagaccag gtgcacgtcc cgcacgtggg
     1741 cgctgtactc gagcaccgac caggtcgcag gagccggccg gtcccgcgcg tcgcggcggc
     1801 tcagcgcgcc gacccaccgc ggcgcgagcg cacggacggt cgtaccgatg gccgtcgggt
     1861 cgacgtccgg cgcggcgtac ccgcactccg ggcaccgctc gtccaggacc caggtccagt
     1921 ccttcgtgtc cggctcgatc gcgggcgggg tctcgtgcga gtcagacgtc atgccagcac
     1981 gctatcgacc actcccggtc gctgcacacc cccgacgggt tgggcgacga gctccgccgt
     2041 caggtcagcg cgacccgcgg ccgcctggct tgccgcgccg ggcggcgggc ttcgcgcgcc
     2101 cgccggaggc tccgcgcgga ccccgcgcgc gctcgtcgcc cgggacccgc ggcgtcggct
     2161 tgcgccgacc gtcgccggcc ttcgcaccgg cggcacccct cgc
//
LOCUS       DASRKH010000192         2046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_483, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000192 DASRKH010000000
VERSION     DASRKH010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..1372)
                     /locus_tag="VFC06_03185"
     CDS             complement(173..1372)
                     /locus_tag="VFC06_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007273073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HZL80936.1"
                     /translation="MATRHEITRKYAKEYERASKKDRGRMLDELVAVTGWSRANARRA
                     VAAAARRRGPARAVQRKPRARTYGYDTLKVLIEVWTLIGEPCGKYLAPIMAATLAQLE
                     AFGELGKVAERLNAQVREQLVTMSPATIDRILKPTKDARYPAAKSATRPGATLRSSIV
                     VRQSMDKMEKAPGFFEIDLVAHCGHTLKGEHAWTLTATDVYLGWTVNIAIRNRAHARV
                     VAAIAQVADRLPYPMVGLDCDNGGEFINHALIAWCAERMIFMTRARAHTSNDNAHVEQ
                     KNGDIVRKSAFRYRYDTPDELALLNELWGYVNLRKNLFLPTRKANGWRSTKAGRNTRT
                     YDEPKTPYQRLTEEAGFLGEETEHRLQELYAQTNPADLTRNINRIQQALIVSAKDKTI
                     ALRAEVS"
     gene            1772..>2046
                     /locus_tag="VFC06_03190"
     CDS             1772..>2046
                     /locus_tag="VFC06_03190"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HZL80937.1"
                     /translation="MATVIDCYTKACIGYAMAEHMRADLVIDALEMASRNYPLAAGAI
                     FHSDRGTQYTSQAFANKTAELGIRRSVGRTGVCFDNAQAESFSGLSIS"
BASE COUNT          299 a          686 c          675 g          386 t
ORIGIN      
        1 cggatgacct ctggcgccac ggccctcctc cggtacggtc gcgggtcgtc agtgtgactg
       61 tctcggggcc ggtcggaggg ccgcgccgac cagtccagtt ggttgccggg tgccgtggtg
      121 cctcatgtca atgtgctcgc gaaacggggt gcgcgtccct cacgtagttt gctcacgaaa
      181 cctcggccct cagggcgatg gttttgtcct tggcggagac gatgagggct tgctggatcc
      241 ggttgatgtt ccgggtcagg tccgccggat tggtctgggc gtagagctcc tgtaggcggt
      301 gttcggtttc ttcgccgagg aagccggcct cctcggtcag ccgctggtaa ggggtcttgg
      361 gttcgtcgta ggtgcgggtg ttgcgtccgg ccttggtgct gcgccacccg ttggccttcc
      421 tcgtgggcag gaacaggttc ttgcgcaggt tcacataccc ccacagctcg ttgagcagcg
      481 ccagctcatc gggggtgtcg tagcggtagc ggaaggcgga cttgcgcacg atgtcgccgt
      541 tcttctgctc cacgtgcgcg ttgtcgttgg aggtgtgcgc ccgcgcgcgg gtcatgaaga
      601 tcatccgctc ggcgcaccag gcgatcaggg cgtggttgat gaactctccg ccgttgtcac
      661 agtccagccc gaccatcggg tacggcagcc ggtcagcgac ctgcgcgatg gccgcaacga
      721 cccgggcgtg ggcgcggttg cggatggcga tgttcacggt ccacccgagg tagacgtcgg
      781 tagcggtcag cgtccaggcg tgctcaccct tgagggtgtg cccgcagtga gcgaccaggt
      841 cgatctcgaa gaaccctggg gccttctcca tcttgtccat cgactgacgt accacgatcg
      901 acgaacgcaa cgtggccccg ggacgggtcg cggacttcgc cgcggggtac cgggcgtcct
      961 tggtgggttt gaggatccgg tcgatcgtgg ccgggctcat cgtcaccagc tgctcacgca
     1021 cctgagcgtt gagccgctca gcgaccttgc ccagctcacc gaaggcctcc agctgggcca
     1081 aggtggcggc catgatcggg gccaggtact tcccgcacgg ctccccgatc agcgtccaca
     1141 cctcgatcag gaccttgagg gtgtcatacc cgtacgtgcg cgcccgtggc ttgcgctgga
     1201 ccgcccgggc cgggcccctg cgcctggccg ccgccgcgac cgcccgccgg gcattggccc
     1261 gcgaccagcc cgtgaccgcg acgagctcgt cgagcatccg gcccctgtcc ttcttcgaag
     1321 cgcgctcgta ctccttcgcg tacttcctcg tgatctcatg acgtgtcgcc atcgacaacc
     1381 gttgacccat cccccaagca caccgcagcg cccgaagcag gacccaggtt tcgcgagcag
     1441 ttctcccgtg aggcacgcgc cccacttcgc gagcacttta ggtgagtctc gtcgacccat
     1501 taactcagaa ctacacgttt ggtattctta tcgtatgcca cgggccggga tgccgagctg
     1561 acccgcctag gcgtgcccgt cggcccggag ctcgtccgga cgctgatgcg ggagctggac
     1621 ctgcggccct gccagccgcg gccgtggcgg ccgaccacga ccgtcgccgg cgacgccgcg
     1681 acggtcccag acctgctgtg ccgcgacttc accgccgacc ggccgggggt gaagctggtt
     1741 ggggacatcc acctggcagg gctggatatt tttggccacc gttatcgact gctacaccaa
     1801 ggcctgcatc ggctacgcga tggccgagca catgagagcc gacctggtca tcgacgcgct
     1861 ggagatggcg agccgcaatt accccctcgc cgccggggcg atattccact ccgaccgggg
     1921 cacccaatac acctcgcagg cgttcgccaa caagactgcg gaactcggga tccgccgctc
     1981 ggtcgggcgc accggggtat gcttcgacaa cgcgcaggcc gaatcattta gtgggctatc
     2041 catttc
//
LOCUS       DASRKH010000193         2674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_54189, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000193 DASRKH010000000
VERSION     DASRKH010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54189"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VFC06_03195"
     CDS             complement(<1..264)
                     /locus_tag="VFC06_03195"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HZL80938.1"
                     /translation="MIVNALTIAGTDPSGGAGIQGDLKTFSALGVYGTAVVTTLVAQN
                     TCGVAAIHEVPASFVTAQLENLLDDVRIDVVKIGLLGTADVVEA"
     gene            complement(261..890)
                     /gene="thiE"
                     /locus_tag="VFC06_03200"
     CDS             complement(261..890)
                     /gene="thiE"
                     /locus_tag="VFC06_03200"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HZL80939.1"
                     /translation="MPLDLTVYLVIGTQGAGRRTMRATVEAAVAGGVSAVQLRDKRAS
                     GRELLALARELLDTLDGTGVPLLVNDRLDVALAAGAHGVHLGQSDLDVRDARRLAGPG
                     FLIGLSVSRAQEIAEVQALPAGTVDYLGIGPVFPTSTKDDAADALGLDAVATLRTGTT
                     LPCVGIGGITQRNARSVWATAMDGLAVVSAICDAADPRAATMVLKSSRP"
     gene            complement(894..1721)
                     /gene="thiM"
                     /locus_tag="VFC06_03205"
     CDS             complement(894..1721)
                     /gene="thiM"
                     /locus_tag="VFC06_03205"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HZL80940.1"
                     /translation="MAATLLPQDASRTASSEDAIRVLDELRRNGPLVHCMTNIVVAGF
                     TANVLLAVGASPAMVENSEESAELAQVADALLVNLGTLSPDKVTAMRAAVAAAGRAGT
                     PWVLDPVAVGALAYRTWFAAELVAGGPAVIRGNPSEVLSLAGATGAAGRGVDSTVSSG
                     DALVRATELARSAQTTVAVSGAVDYLTDGITVLEVHTGHPMMTQVTGVGCALGALVAA
                     CCAVESSPLIASGAATTILTVAAEVAASTSSGPGSFAVGLLDALANLDAATLRAHVG"
     gene            complement(1724..>2674)
                     /gene="thiC"
                     /locus_tag="VFC06_03210"
     CDS             complement(1724..>2674)
                     /gene="thiC"
                     /locus_tag="VFC06_03210"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019484264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HZL80941.1"
                     /translation="QCEQGVDYMTVHAGVLLRYVPLTAGRVTGIVSRGGSIMAAWCLA
                     HHQENFLFTHFVELCAILRRYDVTFSLGDGLRPGSIADANDDAQLAELRTLGELTPIA
                     KSHGVQVMIEGPGHVPMHKIAENVRLEEELCEEAPFYTLGPLATDIAPAYDHITSAIG
                     AAMIAQAGTAMLCYVTPKEHLGLPDRDDVKTGVITYKIAAHSADLAKQHPRAQERDDA
                     LSRARFEFRWDDQFALSLDPDTARAFHDETLPAQAAKTAHFCSMCGPKFCSMRISADV
                     RTYAQENGLTSTEAIEAAMAVKSAEFALRGNSVYLPIEAI"
BASE COUNT          395 a          913 c          993 g          373 t
ORIGIN      
        1 ggcctcgacg acgtcggccg tgccgagcag gccgatcttg acgacgtcga tgcggacgtc
       61 gtcgagcaga ttctcgagct gagccgtgac gaagctcgcg gggacctcgt ggatcgcggc
      121 cacgccacag gtgttctggg cgaccagggt cgtgacgacc gccgtgccgt agacgcccag
      181 ggcagagaac gtcttgaggt ccccctggat cccggccccg ccgctcgggt cggtacctgc
      241 gatggtcaac gcgttgacga tcacgggcgc gagctcttca ggaccatggt cgcggcgcgt
      301 gggtcggccg cgtcgcagat cgccgacacg acagccagcc cgtccatcgc agtcgcccag
      361 accgagcgcg cgttgcgctg ggtgatgccg ccgatgccca cgcacggaag ggtcgtcccg
      421 gtgcgcaagg tcgcgacggc gtcgagcccg agcgcgtcgg cggcgtcgtc cttggtggag
      481 gtggggaaga ccgggccgat gccgaggtag tcgaccgtgc cggcaggcag ggcctgcacc
      541 tcggcgatct cctgtgcgcg cgagaccgag aggccgatca ggaagcccgg accggccagg
      601 cggcgtgcgt cccggacatc gagatcggac tgaccgagat ggacgccgtg cgcgccggcc
      661 gcgagggcga catcgagccg gtcgttgacc aggagcggga caccggtgcc gtcgagcgtg
      721 tcgaggagct cgcgcgcgag ggcgagcagc tcgcgtccgg acgcccgctt gtcgcggagc
      781 tgcacggcgc tcacacctcc ggccacggcg gcttcgacgg tcgcgcgcat cgtgcgcctg
      841 cccgcaccct gcgtgccgat gacgaggtag acggtgaggt cgagaggcac gactcagccg
      901 acgtgcgcgc gcagggtcgc ggcgtcgagg ttcgcgagag catcgaggag gccgaccgcg
      961 aagctgcccg ggcccgacga cgtcgaggcc gcgacctcgg ctgcgaccgt gaggatcgtc
     1021 gtcgcggccc ccgacgcgat caagggtgag ctctcgacgg cgcagcacgc cgccacgagc
     1081 gcgcccagag cgcagccgac gccggtcacc tgggtcatca tcggatggcc cgtgtgcacc
     1141 tccagcaccg tgatgccgtc cgtgaggtag tcgaccgcgc cactcaccgc gacggtggtc
     1201 tgcgcgctgc gggccagctc ggtggcgcgc acgagcgcgt cgcccgagct gacggtggag
     1261 tcgacgccgc gaccagcggc gcctgtggcg ccggcgagcg agaggacctc cgacgggttc
     1321 ccgcggatga ccgcgggacc gccggccacc agctcggcgg cgaaccaggt ccggtaggcg
     1381 agcgccccga ccgcgaccgg gtccaggacc cagggcgtgc cggcacgacc cgctgccgcg
     1441 acggccgcgc gcatcgcagt gaccttgtcc ggtgacagcg tcccgaggtt caccagcagg
     1501 gcgtccgcga cctgggcgag ctcggcggac tcctcggagt tctcgaccat cgccggcgac
     1561 gcccccacgg cgagcaggac gttcgcggtg aaccccgcta cgacgatgtt ggtcatgcag
     1621 tggacgagtg gaccgttccg gcgcagctcg tcgagcacac ggatcgcgtc ctcggacgac
     1681 gcggtccgag acgcgtcctg gggcaggagc gtggcggcca cggctagatc gcctcgatcg
     1741 ggaggtagac cgagttgcct cggagcgcga actcggcgga cttgacggcc atggccgcct
     1801 cgatcgcttc cgtgctcgtg aggccgttct cctgcgcgta cgtccggacg tcggcggaga
     1861 tccgcatcga gcagaacttc gggccgcaca tcgagcagaa gtgggccgtc ttcgcagcct
     1921 gcgccggcag ggtctcgtcg tggaacgccc gcgcggtgtc cgggtcgagg gacaacgcga
     1981 actggtcgtc ccagcggaac tcgaagcggg cccggctgag cgcgtcgtcg cgctcctgag
     2041 cccgggggtg ctgcttcgcg aggtctgccg agtgcgccgc gatcttgtag gtgatgacgc
     2101 cggtcttcac gtcgtcgcgg tcgggcaggc ccagatgttc cttgggggtg acgtagcaca
     2161 gcatcgcggt gccggcctgg gcgatcatcg ccgcgccgat cgcggaggtg atgtggtcgt
     2221 acgcgggcgc gatgtcggtc gcgagcggac cgagcgtgta gaacggagcc tcctcgcaga
     2281 gctcttcctc gagccggacg ttctccgcga tcttgtgcat cggcacgtgg ccggggccct
     2341 cgatcatcac ctgcacgcca tgggacttcg cgatcggcgt gagctcgccc agcgtgcgca
     2401 gctcggcgag ctgggcgtcg tcgttggcgt cggcgatcga ccccggacgt agcccgtcgc
     2461 cgagcgagaa cgtcacgtcg tagcgccgca ggatcgcgca gagctcgacg aaatgggtga
     2521 acaggaagtt ctcctgatgg tgcgcgagac accaggcggc catgatcgag ccgccgcgcg
     2581 agacgatccc cgtcacgcgc cccgcggtca gcggcacgta ccggagcagc acgcccgcgt
     2641 ggaccgtcat gtagtcgacg ccctgctcgc actg
//
LOCUS       DASRKH010000194         2276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_10135, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000194 DASRKH010000000
VERSION     DASRKH010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10135"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="VFC06_03215"
     CDS             complement(<1..917)
                     /locus_tag="VFC06_03215"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HZL80942.1"
                     /translation="MRHHQGMEKLTIDGVYAAAETLRDLLPPTPAWSYPVLDAAVGSR
                     AIVKHENVQPTGAFKVRGGLNLLATLSPTERAAGLITVSTGNHAQSLAYAASRSGVAA
                     TIVMPRSTPFGKVEAVRSWGARALLEGDDMAQAADFAVDLAAREGLHFVNPGNTPAIV
                     YGHGTVYLELLQSHPEIETLYVPVGSGSGLAGALLVRDAIAPGTRVVGVQAEGAAAAY
                     ESWRAGTVKTVEAHTFAAGLATGSGFELPQSIIRERLDDFLLVSDDDLRHAMSLMATA
                     AHTLCEGAGAGGLAARQSSNNLRGADAST"
     gene            984..1898
                     /locus_tag="VFC06_03220"
     CDS             984..1898
                     /locus_tag="VFC06_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZL80943.1"
                     /translation="MIDVTRLQVLVALAKYGTASAAAEALHYSQPTVSHHLKRLEAET
                     GAVLMRRVGRGLVLTDEGVRLAARGEEILGLMSRASTELASAVSLQSGHVRLAIFPSG
                     MATLAPRIVTELAERHPGLQLDIAEAEPPEAERLLIEGSVDLAITFTYPDYECSDAVT
                     SEVMGEDPLYLVTPPRKHVDGTMAVAGLAAFQDEDWMAGCERCRGYLVSACAQADFTP
                     TIRFASDDYVAVQALIAVGHGVTMLPGLALAAHRHPDVEVTRVEGAVRSLRLLSLGRP
                     PLPPALDAASRVAREVVTAVVGASSLQR"
     gene            2067..>2276
                     /locus_tag="VFC06_03225"
     CDS             2067..>2276
                     /locus_tag="VFC06_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80944.1"
                     /translation="MIVNDLDTGKGIVRWRESGDPDAPITAIWHHGTPGIAGLAPAND
                     ALACVEVVDNHGPTLRRPPFGHGGTR"
BASE COUNT          342 a          811 c          782 g          341 t
ORIGIN      
        1 ctggtggacg catccgcgcc acgaaggttg tttgaggact gacgagctgc caagccgcct
       61 gcgcccgcgc cctcgcacaa ggtgtgcgcc gcggtcgcca tgagcgacat cgcgtggcgc
      121 aggtcgtcgt ccgagaccag caggaagtcg tcgagccgct cgcgtatgat cgactgcggg
      181 agctcaaacc cggaacccgt ggcgagcccc gcggcaaagg tgtgggcctc cacggtcttc
      241 acggtgccag cgcgccaaga ctcataggca gctgcggcgc cctcggcctg cacgcccacc
      301 acccgcgtcc cgggcgcgat cgcgtcgcgc accagcagcg cgccggccag gcccgagccc
      361 gagcccacgg gcacgtacag cgtctcgatc tcggggtgcg actggagcag ctccaagtac
      421 acggtcccgt ggccgtagac gatcgcgggc gtgttgcccg ggttcacaaa gtgcaggccc
      481 tcgcgcgcgg ccaggtcgac cgcgaagtct gcggcctggg ccatgtcgtc gccctccagc
      541 agggcgcgcg cgccccagga gcgcactgcc tccaccttgc cgaacggcgt gctgcggggc
      601 atcacgatgg tggccgcgac gccggatcgg gaggcggcgt acgccaggga ctgggcgtga
      661 ttgccggtgg agacggtgat cagcccggcc gcgcgctcgg tgggggagag cgtcgccagc
      721 aggttgagac cgccgcgcac cttgaaggcg ccggtgggct ggacgttctc gtgcttgacg
      781 atggcgcggg agcccacggc cgcgtcgagc accgggtagg accaggcggg cgtggggggc
      841 agcaggtcgc gcagcgtttc ggccgcggcg tagacgccat caatagtgag cttctccatg
      901 ccttgatggt ggcgcacgaa cgatgcatca gtccaatgat agttatcgac tataaccata
      961 gacgccgttg ataggatgag tgcatgatcg acgtgacccg cctccaagtt ctcgtggccc
     1021 tggccaagta cggcaccgca tcggccgctg ccgaagcgct ccactacagc cagcccaccg
     1081 tctcccacca cctcaagcgc ctcgaggccg agacgggcgc cgtgctgatg cggcgcgtcg
     1141 gccggggact ggtgttgacc gacgagggcg tgcgcctcgc ggctaggggc gaggagatcc
     1201 tcggcctcat gtcgcgcgcg tccaccgagc tcgcgtcggc cgtgtccctc cagagcggcc
     1261 acgtgcggct cgcaatcttc ccctccggca tggcgacgct cgccccgcgc atcgtcaccg
     1321 aactggccga gcgccacccc ggactgcaac tcgacatcgc ggaggccgag cctcccgagg
     1381 cggaacggct cctgatcgag ggctccgtgg acctggccat caccttcacc taccccgact
     1441 acgagtgcag cgacgccgtc acatcggagg tcatgggcga ggaccccctg tatctggtca
     1501 cgcccccgcg caagcacgtc gacggcacga tggcggtcgc gggtctggcc gccttccaag
     1561 acgaagattg gatggcgggt tgcgagcggt gccgcggcta cctggtgagc gcgtgcgccc
     1621 aggcagactt cactccgacc atccgcttcg cctcggacga ctacgtggcc gtccaggcgc
     1681 tcatcgccgt gggccacggg gtcacgatgc ttcccggcct cgcgctggcc gcgcatcgtc
     1741 atcccgacgt cgaggtgacg cgcgtcgaag gcgcggtgcg ctcgttgcgg ctgctgtcgc
     1801 tcggccgccc gccgttgccg ccggcgctcg acgccgcgag ccgcgtggcg cgggaggtcg
     1861 tcactgccgt cgtcggggcg tcgtccctgc aaaggtagga caatcctggg aactgagtcc
     1921 cgcgtcgagc gccgccaggg cgcctatcct gacgacacgc ccgtgccctt cgcaaccact
     1981 ttccccctgg ttccccgtcc gctttcagcg tgtgcggagg cgcgggttcc cccatgcccg
     2041 aatggggggc ggcgtagggt cggaccgtga ttgtcaacga cctcgacaca ggcaagggca
     2101 tcgttcgctg gcgcgagtcg ggcgatcccg acgcacccat caccgcgatc tggcaccacg
     2161 gcacgcccgg gatcgccgga ctcgcgccag cgaacgatgc ccttgcctgt gtcgaggtcg
     2221 ttgacaatca cggtccgacc ctacgccgcc ccccattcgg gcatggggga acccgc
//
LOCUS       DASRKH010000195         2990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_41997, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000195 DASRKH010000000
VERSION     DASRKH010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41997"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VFC06_03230"
     CDS             <1..365
                     /locus_tag="VFC06_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HZL80945.1"
                     /translation="DSAVRRYVADAGPLLERLHRLTRADCTTRNQRKAIRLAAAYDDL
                     EDRIALLREDEELRAIRPELDGTQIMEILGVPAGPVVGRAYKYLLDVRMEEGPIGADA
                     ARERLLRWWAAEGGRDAG"
     gene            355..1461
                     /locus_tag="VFC06_03235"
     CDS             355..1461
                     /locus_tag="VFC06_03235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HZL80946.1"
                     /translation="MRDDATPRPATPPAPHTHRGARDVRETDGSLLPRRTAARRYALR
                     RSFTEFVRDQGLDEAAALTYNSVVSLLPAVLVMLAVLGLVGQGEPARRAILDFIHELL
                     PGDVADVLSEPIQDFTSKGGAGWILVLTLVVALWFASNYVSAFGRAMNRVYQVEEGRP
                     AWQLNLFGYMLTAVLITMLAVVVSLLVVSGRFAAALVRTLELDSSTVVMWQVGRWPIL
                     LVLVITAIALLYNLTPNIHPPGFHWLSPGVICALVVGGLATGGMALFLRNFPNYNYYY
                     GALTGVLLFLLWLFSINAALLFGARLNAELERGRELERGFGAEREILLPPRSTMTSAR
                     RAVKYEGLIARATALRVSRGETSDPDSVPPEPWA"
     gene            complement(1514..2593)
                     /locus_tag="VFC06_03240"
     CDS             complement(1514..2593)
                     /locus_tag="VFC06_03240"
                     /EC_number="5.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011775542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004512 - inositol-3-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006021 - inositol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HZL80947.1"
                     /translation="MSSIRVAIVGVGNCASSLVQGIHFYRDADPDTKVPGLMHVVFGD
                     YHVHDIDIVVAFDVDAKKVGLDLSDAIGASENNTIKFCDVPHLGVTVQRGTTLDGLGK
                     YYRETIDESDAEPVDVVKVLKDAKVDVVVNYLPVGSEQAARFYAQCAIDAGCGFVNAL
                     PVFIASTEEWARKFEDAGLPIIGDDIKSQVGATITHRVLAKLFEDRGVELMRTYQLNV
                     GGNMDFKNMLERDRLQSKKLSKTQAVTSNIDIKLEDRNIHVGPSDYVAWLDDRKWAFV
                     RLEGKTFGDVPLNLEYKLEVWDSPNSAGVIIDAIRATKIGLDRGIGGPLLSPAAYFMK
                     SPPEQRDDDTGRDQVEAFIRGEVER"
BASE COUNT          447 a         1002 c         1041 g          500 t
ORIGIN      
        1 ccgactccgc ggtgcgccgc tatgtggccg acgccggccc cctgctcgag cggctgcacc
       61 ggctcacgcg cgcggactgc accacccgca accagcgcaa ggccatccgt ctcgccgccg
      121 cctacgacga cctcgaggac cgcatcgccc tcctgcgcga ggacgaggag ctcagggcca
      181 ttcggcccga gctcgacggt acccagatca tggagatcct cggcgtcccc gccggccccg
      241 tggtggggcg agcctacaag tacctgctcg acgtgcggat ggaggagggc ccgatcggcg
      301 ccgacgccgc ccgcgagcgc ctgctccggt ggtgggccgc cgagggtggt cgcgatgcgg
      361 gatgacgcca cgccccgccc cgcgacgccg cccgcaccgc acacgcaccg gggcgcgcgt
      421 gacgtccgtg agactgacgg ctccctcctt ccccggcgga ccgccgcccg gcggtatgcg
      481 ctccggcgct ccttcaccga gttcgtccgc gaccagggcc tcgacgaggc ggccgcgctc
      541 acgtacaact cggtggtctc actgctgccg gcggtgctcg tgatgctcgc cgtgctcggg
      601 ttggtgggac agggcgaacc cgcgcggcgg gccatcctcg acttcatcca cgaactcctg
      661 cccggtgacg tggcggatgt cctctcggaa ccgatccagg acttcacctc caaggggggc
      721 gcggggtgga tcctggtgct cacgctggtg gtggcgctct ggttcgcctc gaactacgtg
      781 agcgcattcg gccgggcgat gaaccgggtg taccaggtgg aggagggacg ccccgcctgg
      841 cagctgaacc tcttcgggta catgctgacg gccgtgctca tcaccatgct ggccgtggtc
      901 gtgagcctgc tggtggtgag cggccgattc gcggcggcgc tcgtgcgcac gctggagctc
      961 gacagctcga ccgtggtgat gtggcaggtg ggcaggtggc ccatcctcct ggtgctggtg
     1021 atcaccgcga tcgcgctgct ctacaacctc accccgaaca tccacccccc ggggttccac
     1081 tggctgagcc cgggagtgat ctgcgcgctg gtggtgggcg ggctcgcgac gggcgggatg
     1141 gcgctcttcc tccggaactt cccgaactac aactactact atggcgccct gaccggcgtg
     1201 ctcctcttcc tgctgtggct cttctccatc aatgcggcac tgctcttcgg tgcgcggctc
     1261 aacgcggaac tggagcgggg ccgcgagctc gagcggggtt tcggggcgga gcgggagatc
     1321 ctcctcccgc cgcgcagcac gatgacgagc gcgaggcggg cagtgaagta cgaggggctc
     1381 atcgcccgcg ccaccgcgct gcgggtgagc cgtggcgaga cctcggatcc ggactccgtc
     1441 ccgccggaac cctgggcctg acccgccggg ccccatcagg ggccgagggt gcctggccca
     1501 ggggccgaga gtgctagcgc tccacctcgc cgcggatgaa ggcctccacc tggtcgcggc
     1561 cggtgtcgtc gtcgcgctgc tccgggggcg acttcatgaa gtacgcggcc ggggagagca
     1621 gcggaccacc gatgccgcgg tccagaccga tcttggtggc gcggatggcg tcgatgatca
     1681 cgccggcgga gttgggggag tcccacacct cgagcttgta ctcgaggttc aggggcacgt
     1741 cgccgaaggt cttgccctcc aggcggacga aggcccattt gcggtcgtcc agccaggcca
     1801 cgtagtcgct cgggccgaca tggatgttgc gatcctcgag cttgatgtcg atgttggagg
     1861 tcacagcctg ggtcttggag agcttcttgg actgcaggcg atcccgctcg agcatgttct
     1921 tgaagtccat gttgccgccg acgttgagct ggtaggtccg catgagttcc accccgcggt
     1981 cctcgaacag cttcgcgagc acccggtggg tgatcgtggc gcccacctgg gacttgatgt
     2041 cgtcgccgat gatcggcagg ccggcatcct cgaacttccg ggcccactcc tcggtggagg
     2101 cgatgaagac gggcagggcg ttcacgaagc cgcacccggc gtcgatggcg cactgcgcgt
     2161 agaagcgggc ggcctgctcg gacccgaccg ggaggtagtt gaccaccacg tcgaccttgg
     2221 cgtccttgag gaccttgacg acgtcgaccg gctcggcgtc cgactcgtcg atcgtctcgc
     2281 ggtagtactt cccgagtccg tcgagggtgg tgccgcgctg caccgtcacg cccaggtgag
     2341 ggacgtcgca gaacttgatg gtgttgttct cagaggcgcc gatggcgtcg gagaggtcca
     2401 gcccgacctt cttggcgtcg acgtcgaaag cgacgacgat gtcgatgtca tggacgtggt
     2461 agtcaccgaa cacgacgtgc atgagaccgg ggactttggt gtccgggtcg gcgtcccgat
     2521 agaagtgaat gccttggacc aacgacgacg cgcagttgcc gacacccacg atggcaacgc
     2581 ggattgagct cattgatgct cctagagtga tgggactgct gatcgaatcc cgagatggtc
     2641 aacccttcga tcatagccac cgttttcggc gtgccccagc gagggtccgc ccgcgggggc
     2701 gtggggttcg tcgcagtcgt cgccaccatc gccggccaca ccctctcgag acgtcacggg
     2761 cgagggcttc tgctggacga cgtcgccccg cgagtgtgag atcggtcgct ggatcgcccc
     2821 aaacggccgc ggcccgccca tacttgtgga agaacaggca tgtcgccagg gcgacgagga
     2881 catgtcgagg cctcgggacg cgcgcttccg tcgccgagag gaagcaggag cagtggctga
     2941 tcccaaagga acgtcccagt acccaccggt gcgccgcacc tcggtgaagg
//
LOCUS       DASRKH010000196         2296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_47720, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000196 DASRKH010000000
VERSION     DASRKH010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47720"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VFC06_03245"
     CDS             complement(<1..393)
                     /locus_tag="VFC06_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HZL80948.1"
                     /translation="MSINSLGSQSTLNVGEKSYEIYRLDAVPGLEKLPYSLKILAEAL
                     LRTEDGANITADHVKALAQWDPNATPDTEIQFTPARVVMQDFTGVPCVVDLATMREAV
                     VELGGDPSVINPLAPAEMVIDHSAGAKGL"
     gene            complement(443..1633)
                     /locus_tag="VFC06_03250"
     CDS             complement(443..1633)
                     /locus_tag="VFC06_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HZL80949.1"
                     /translation="MTQTIRLTVGAPAHGGHCIARHEGRAVFVRGGLPGEVVQARVDD
                     PAADARFWNAEVTEVLEASPDRVEHPWPEAAVGGVGGAELGHVALPAQREWKLAVVRE
                     SLSRFAGIDYEGDVRAAPGDDERGGLRYRTRVSATAGEDGRATMAVPGSGVRHTLKRM
                     PLAVQEAEEALLATKTEPGQLIEVATTAFGETYVSLGKGDPTLLTETVTVGDRSFAYA
                     LRAGEFWQVHREAPAVLLGEVMRLVGDAPRVLDLYSGAGLFAVPFAAEGRDVTAVELA
                     ATHSKGKSSLQQNLRGYPGATAVAGDTRRTVAAMAAADQDWSRGAVVLDPPRSGAKAA
                     TIEAIASLEPARIVYVACDPVALGRDAKTLAAAGYVVEEIQSFDLFPMTHHIETIASF
                     ARAK"
     gene            complement(1630..>2296)
                     /locus_tag="VFC06_03255"
     CDS             complement(1630..>2296)
                     /locus_tag="VFC06_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HZL80950.1"
                     /translation="RRLLHIVALVVAVGVLAVTAVFNFANGAWIAIVLIVLVVIMMHA
                     IKRHYGAVRADIALDREAKAPSLPSATHGIVLVAQMHRPALHALAYAKAARHSSLKAV
                     TVQIDRGAAVALQRRWASMDLGAPLVVLDSPYRDLVSPMLEYVKNMHRASPREAVFVY
                     VPEYVVGRWWEQLLHNRSTARLRVQLLNVPNVVVSAVPWMLESARREGPTDDLVIEGP
                     EPQ"
BASE COUNT          373 a          791 c          751 g          381 t
ORIGIN      
        1 caaccccttg gcgcctgcgg agtgatcaat caccatctcc gcaggcgcca aggggttgat
       61 gacggaaggg tcgccgccca attccacgac ggcctcgcgc atcgtcgcaa ggtcgaccac
      121 gcaagggacg ccggtgaagt cctgcatcac cacacgggcg ggggtgaact ggatttccgt
      181 gtccggagtg gcgttggggt cccactgcgc caaggccttg acgtggtcgg ccgtgatgtt
      241 ggcgccgtcc tcagtccgta gcagggcctc ggccaggatc ttcagcgagt acgggagctt
      301 ctccaagcct ggcactgcgt cgaggcggta gatctcgtac gacttctccc ccacattgag
      361 ggtggactgt gatcccaggc tgttgatgct cattccgctc cttgattcgt ggcgtctcat
      421 cctaccgata cgggcatgtt cgctattttg cgcgcgcgaa gctggcgatt gtttcgatgt
      481 ggtgcgtcat ggggaacagg tcgaaggact gaatctcctc caccacgtag cccgctgcgg
      541 caagggtctt ggcgtcccgg ccgagcgcca cggggtcgca cgccacatag acgatgcgcg
      601 cgggctccag cgaagcgatc gcctcaatcg tggcggcctt cgcccccgac cgcggcgggt
      661 cgagcacgac cgcgccgcgc gaccaatctt ggtcagccgc ggccatggct gcgaccgtgc
      721 gacgggtgtc gccggccacg gcggtggctc cggggtagcc acgcaggttc tgctggaggc
      781 tggacttgcc cttggagtga gtggccgcca actccacggc cgtcacgtcc cggccctcag
      841 ccgcgaacgg gacggcgaac agccccgccc ccgaatacag gtcgagcacg cgtggcgcat
      901 cgcccacaag gcgcatgacc tcgccaagaa gcacggccgg agcctcgcgg tgcacttgcc
      961 agaactcgcc cgctcgcaag gcgtaggcga aactgcggtc tccgaccgtg accgtctcgg
     1021 tcaggagcgt ggggtcgccc ttgccgagcg acacgtacgt ctcgccaaag gcggtggtgg
     1081 ccacttcaat gagttggccc ggctctgtct tagtcgccag cagagcctct tcggcctcct
     1141 gcaccgcgag cggcatcctt ttcagagtgt gcctgacacc gctgccgggt accgccatgg
     1201 tcgcccggcc gtcttcgccc gcggtcgccg acaccctggt ccggtatcgc agcccgccgc
     1261 gttcatcgtc gcccggcgcg gcgcgcacgt cgccctcgta gtcgattccc gcgaagcggg
     1321 acaaggattc acgcaccacc gcgagcttcc actcgcgctg ggccggcaac gccacgtgac
     1381 cgagctccgc gcccccgaca ccgccgacag cggcctcggg ccacgggtgc tcgaccctgt
     1441 caggcgaggc ttcgagaacc tccgtgacct ccgcgttcca gaaccgtgcg tccgcggctg
     1501 ggtcgtccac tcgcgcctgc accacctcac cgggaaggcc accgcgtaca aacaccgcgc
     1561 gcccttcgtg ccgtgcaatg cagtggccgc cgtgcgcggg ggcaccgacg gtaaggcgaa
     1621 tggtctgggt cattggggct ccggtccttc gatgacgagg tcgtctgtag ggccctcgcg
     1681 gcgcgcggat tcgagcatcc acggcaccgc gctcaccacc acgttgggca cattgagcaa
     1741 ttggacgcgc aatcgcgccg tcgaacgatt gtggagcagt tgctcccacc atcggcccac
     1801 gacgtattcg ggcacgtaga caaagaccgc ttcgcgcggc gaggcgcggt gcatgttctt
     1861 gacgtactcg agcatcgggc tcacgaggtc acgatacgga gaatcgagca ccacgagcgg
     1921 cgcacccagg tccatgctgg cccagcggcg ctgcaatgcg acggccgctc cccggtcgat
     1981 ctgtactgtc acggccttga ggctgctgtg gcgcgccgcc ttggcgtacg cgagggcgtg
     2041 gagggccggc cggtgcattt gggcgacgag cacgatgccg tgcgtcgccg acggaaggct
     2101 gggagccttc gcttcgcggt cgagcgcgat gtccgccctc acggcgccat agtgacgttt
     2161 gatggcatgc atcatgatca cgaccagcac gatcagcacg atcgcaatcc acgcgccatt
     2221 ggcgaagttg aacacggcgg tcaccgcgag cactcctaca gccaccacca aggcgacgat
     2281 gtggagaagc cggcgc
//
LOCUS       DASRKH010000197         2591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_55455, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000197 DASRKH010000000
VERSION     DASRKH010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55455"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..301
                     /locus_tag="VFC06_03260"
     CDS             101..301
                     /locus_tag="VFC06_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80951.1"
                     /translation="MTAAFEDAHSAADKVDAVPELEETVACRMTLGVQAVTKMAPSVT
                     RGTDERDRPDGGGDAGMSATVE"
     gene            complement(380..583)
                     /locus_tag="VFC06_03265"
     CDS             complement(380..583)
                     /locus_tag="VFC06_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80952.1"
                     /translation="MDRSEKAREKRLRRLAASQGYRLDKSRRQDWRAADCGAYWLVCA
                     ETDILATWEAGISLDEVEAWLTS"
     gene            complement(974..2236)
                     /gene="ltrA"
                     /locus_tag="VFC06_03270"
     CDS             complement(974..2236)
                     /gene="ltrA"
                     /locus_tag="VFC06_03270"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HZL80953.1"
                     /translation="MSELKSAGKSFEISKWEVWEAWEKVKANQGAPGVDGCSIEEFEK
                     DLKNGLYRVWNRMSSGSYFPPPVRAVEIPKSHGGGTRILGVPTVADRIAQTVVARRLE
                     ARTEPVFHPDSYGYRPRRSALHAVETCRRRCWKSKWVIDLDIQKFFDSVPWDLVVKAV
                     RAHTEDPWVVLYVKRWLQAPVQQSDGTMLVRDRGTPQGSAVSPVLANLFLHYAFDAWM
                     ARRFPGVAFERYVDDAVVHCVSEAQARLVLTAITARMEQVGLRLHPDKTKIVYCQDSN
                     RRGSYEHTEFSFLGFTFRRRGAWGNQGTLFCSFQPAISKDALNRINAEVRSWRLHNRT
                     GHDLAGLAARINPIVRGWMQYYGAFYRSAIYPLLQRINAYLVRWIRKKYKRLRAKKKA
                     FRCWRGIVKRAPRLFAHWEWVPSVPSVW"
BASE COUNT          464 a          906 c          758 g          463 t
ORIGIN      
        1 ggccggcatg gacttctcca cgttcaagtc atcaatcatg gactgagccg cagactcaat
       61 cgccgagccg gccggcgcca ccgtgacgac cggaatgaac atgactgccg cctttgagga
      121 tgcccactcc gcagccgaca aagtcgatgc tgtccccgaa ctcgaagaaa ccgtcgcgtg
      181 caggatgacg ctcggcgtgc aggcggtgac gaagatggcc cccagcgtga cccgcggcac
      241 ggatgagcgc gaccgtccag atggcggcgg ggatgcgggg atgtcggcga ccgtcgagta
      301 ggcggccgag tatccttggt gaagggcttg gacgctgagc tgttgcgggc atggtcgccc
      361 gccgttcact ggcagcggct cacgacgtca gccacgcctc cacctcgtcc agagatatgc
      421 cggcctccca cgtcgcgagg atgtcggtct ccgcgcagac aagccagtag gcgccacagt
      481 cagccgccct ccagtcctgc cgccggctct tgtccagccg gtatccttgg cttgcggcca
      541 ggcggcggag ccgcttctcc cgcgccttct ctgatctgtc cacgtctgtc cacgcccgcc
      601 taccagatcg cccagatgac gacgactacg gccgcccaga tcgccgcgcc gacgaggccg
      661 gccagcggcc agcttgcgag cttctgacga gtagcgttgg ccatggcgct gtcaatacgg
      721 acggtgccta cgggtcgtgt ggtgtgcaac cgacgcgcgg ccccgcctgc gcgcagtgtc
      781 ggccggtgag cacgccgggt ggagtcgagg ccgggaagtt cacccgtttg gcggtagtga
      841 gtgtccgacg gaactcgggc ctgcctcaaa tccacacgat ctagcgaggt cgggtggccc
      901 ggggcatttc agccccgggc tcccgcagat cccggcgtaa cagtctcccg ttaccgggct
      961 cttgtcatcc tgatcaccag acagacggga cggaaggaac ccattcccag tgcgcgaaca
     1021 ggcgcggggc tcgcttgacg atcccccgcc aacaccggaa ggccttcttc ttcgcccgca
     1081 gccgcttgta cttcttgcgg atccaccgca ccaggtaggc gttgatgcgt tgcaggagcg
     1141 ggtagatcgc cgaacggtag aacgcaccgt aatactgcat ccaaccgcgc acgattgggt
     1201 tgatgcgtgc cgccagtccg gccaggtcgt ggccggtgcg gttgtgaagt cgccaggacc
     1261 gcacctccgc gttgatcctg ttcagggcat ccttgctgat cgcgggctga aaggaacaaa
     1321 acagcgtccc ttggttgccc catgctccgc gccggcggaa ggtgaacccc aagaaactga
     1381 actccgtgtg ctcgtaggag ccccggcggt tgctgtcctg gcagtacaca atcttcgttt
     1441 tgtcggggtg caaccgcaac ccgacctgtt ccatccgcgc cgtgatcgcc gtcagcacca
     1501 ggcgtgcctg ggcttcgctg acgcagtgca cgaccgcatc atccacatag cgctcgaatg
     1561 cgacgcccgg aaacctacgg gccatccacg catcgaaggc gtaatggagg aacagattgg
     1621 ccagcacggg cgataccgcg gatccctgtg gggttccccg gtctcgcacc agcatggtcc
     1681 cgtcggactg ctgcaccggc gcttggagcc accgtttgac gtacagcacc acccacgggt
     1741 cctcggtgtg agcccgtacc gctttgacga cgagatccca cggcacgctg tcgaagaact
     1801 tctggatgtc caaatcgatc acccatttgc tcttccagca gcgcctgcgg cacgtctcga
     1861 ccgcgtgcag cgccgaacgc cgcggccggt agccgtacga gtcgggatgg aagaccggtt
     1921 cggtcctcgc ctccaaccta cgcgcgacca ccgtctgggc gatccgatct gccacggtgg
     1981 gcacccctaa aattctggta cccccgccat gcgacttggg tatttccacc gcccgcaccg
     2041 ggggcgggaa gtaactcccc gacgacatcc gattccagac ccggtacaga ccgttcttca
     2101 ggtccttctc gaactcctcg atcgagcacc cgtccactcc cggcgcaccc tgattggcct
     2161 tgaccttctc ccaggcctcc catacttccc acttcgaaat ctcaaacgac ttgcctgccg
     2221 acttcaattc gctcacgcga ctccttccgg acaattcccg gttgatcgaa cgaacaaacc
     2281 acgaatgacc tgaccccttg gctccgctcc caccgcgtca agcaggaact tcacagctac
     2341 tacgagtcag tccgccggcg caccccgcat cggtactcga tccctcacgg tttcagccgc
     2401 tcgggacact ccctctcgcc cactcctcac aaagcggaca gtatcgagac gcgccttctc
     2461 ccgttccctg cggaagcagc ctgatcaggc tcacgttgcc tatatgccgg acgccgcctg
     2521 gccaataggc gggcacccgc caggcttatc ccgagattgt gtggacaccc cggtttagac
     2581 gtcatctgca g
//
LOCUS       DASRKH010000198         2642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_57916, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000198 DASRKH010000000
VERSION     DASRKH010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57916"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VFC06_03275"
     CDS             <1..307
                     /locus_tag="VFC06_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015780465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZL80954.1"
                     /translation="AILQTLTHGDKRVRDLTEQLGLAQSTVSAHLACLKDCGLVDSRP
                     VGRASMFSLTTAREVLEVLAAAEHLLGATGYRVALCPVYGLGHQDQPLQQAIEVSAL"
     gene            337..2280
                     /locus_tag="VFC06_03280"
     CDS             337..2280
                     /locus_tag="VFC06_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HZL80955.1"
                     /translation="MAPSDDHDDHDVVERIWDVSEIRAGISAGVLLAAAFALSRAGQG
                     GWALGLNLAALLVGASTFVPGALVRVLRGRIGVGTLMTIAALGAVVLGRVGEAATLAF
                     LYSLSEGLEEYSLAKTRGGLRALLSLVPQTATVRRDGVERTVAPDELAVGDVMLVKPS
                     ERLATDGVIRSGHAALDVSAITGESIPVEAGPGSEVFAGSINGTGALEIDVTTTAENN
                     SLARIVHIVEAEQSRKGATQRLADTVARPLVPAIMVTSVLIAVIGSVAGDPALWIERA
                     LVVLVAASPCALAISVPVAVISAVGAASKFGVLIKGGAALETLGKVRVIALDKTGTLT
                     RNHPAVVEVATVGHTREDVLDLAAALEARSEHPLARAILNVAAHVPAAVDVVAVPGSG
                     LTGTIEGRTIRLGRPGWIDPGLLSGDIARMQRAGATAVLVEDDGCVMGAIAVRDELRP
                     EASAVVAELRRHGYSITMLTGDNATTALAVAADAGIDQVHADLRPEGKSALVARMRRD
                     GITAMVGDGVNDAPALATADLGIAMGAMGSDVALETADVALMGEDLRNLPQAFAHARY
                     ARAIMLQNVGLSIALIAILIPLALAGVLGLAAVVLVHELAEVVVIANGVRAGRAQRLL
                     IGGTASVPEPAVAAARVRLGLES"
     gene            complement(2384..>2642)
                     /locus_tag="VFC06_03285"
     CDS             complement(2384..>2642)
                     /locus_tag="VFC06_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HZL80956.1"
                     /translation="FEIGGAWLVWVGAGAVALGLSGLVVTLQPSADFGRILAAYGGVF
                     IAGSLAWGMLVDGFRPDRWDIAGALICLVGVAVLMYAPRPS"
BASE COUNT          387 a          938 c          926 g          391 t
ORIGIN      
        1 ggcgatcttg cagaccctga cccacggtga caaacgggtt cgggacctca ccgagcagct
       61 ggggttggcg cagtcgacgg tcagcgcgca cttggcctgt ttgaaggact gcggcctggt
      121 cgactcgcgg cccgtcggtc gggcgtcgat gttctccctg accaccgcgc gagaggtgct
      181 agaggtcctg gcggccgccg agcacctgct cggagcgacc ggataccggg tcgccttgtg
      241 ccccgtctac ggccttggtc atcaggacca gccgctacag caggcgatcg aggtgagtgc
      301 cctgtgagtg acgcgtgctg cggcgaggag accacggtgg cgcccagtga cgaccatgac
      361 gaccacgatg ttgtggagcg catctgggac gtcagtgaga tccgcgcggg gatcagcgcc
      421 ggcgtgctgt tggcggcggc cttcgccctg agccgtgctg gccagggcgg gtgggcgctg
      481 gggctcaacc tggctgcgct cctggtcggg gcaagcacgt tcgttcccgg cgccctggtg
      541 cgcgtgcttc ggggcaggat cggcgtcggc accctgatga cgatcgcagc tctcggcgcg
      601 gtcgtgctcg gccgggtcgg cgaggccgcg acgctcgcct tcctgtactc gctcagcgag
      661 ggtctggagg agtactccct ggccaagacc cgcggcggcc tcagggcgtt gctctcgctt
      721 gtcccgcaga cggcgaccgt ccgccgagac ggtgtcgagc ggacggtcgc accagatgag
      781 ctcgcggtgg gcgacgtcat gctcgtcaag ccgagcgagc ggctggccac cgacggcgtc
      841 atccgctccg gccacgccgc gctggacgtc tcggccatca ccggtgagtc gatacccgtg
      901 gaagcaggtc cgggcagcga ggtcttcgcc gggtccatca acggcaccgg ggctctggag
      961 atcgacgtca ccacgaccgc ggagaacaac tccctggccc ggatcgtcca catcgtcgag
     1021 gccgagcagt cccgcaaggg cgccacccag cgcctggccg acaccgtggc caggccgctc
     1081 gtcccagcga tcatggtcac gtcggtcctc atcgcggtca tcggatcggt cgccggagac
     1141 ccagcgctgt ggatcgagcg cgccctcgtc gtgcttgtcg cggcctcacc gtgcgcgctg
     1201 gccatctcgg tgcccgtcgc ggtgatttcg gccgtcggcg ccgcgtcgaa gttcggcgtc
     1261 ctgatcaagg gtggggccgc actggagact ctgggcaagg tgcgcgtgat cgcgctggac
     1321 aagaccggca ccttgacccg caaccatccc gccgtggtcg aggtcgccac cgtgggccac
     1381 acccgcgagg atgtcctcga cctcgctgcc gcgctcgagg cccgcagcga gcaccccctg
     1441 gcccgagcca tcctcaacgt cgcggcgcat gtccccgctg cggtcgacgt cgtggcagtc
     1501 cccggttccg gtctgaccgg aaccattgag ggccggacca tccggctcgg gcggcccggc
     1561 tggatcgacc cgggcctgct gtccggtgac atcgcccgga tgcagcgcgc cggagccacc
     1621 gccgtcctgg tcgaggacga cggctgcgtc atgggagcga tagccgtccg cgacgagctt
     1681 cgacccgagg cgtccgcggt cgtcgcagaa ctgcggcggc acgggtactc catcaccatg
     1741 ctcaccggcg acaacgccac caccgccctg gccgtcgccg ccgacgccgg catcgaccag
     1801 gtccacgccg acctgcgccc ggagggcaag tcggcactcg tcgcacggat gcgacgcgac
     1861 gggatcaccg ccatggtggg cgacggcgtg aacgacgccc ccgccctggc taccgcggac
     1921 ctcggcatcg ccatgggcgc catgggatcc gacgtcgcgc tcgagaccgc cgacgtggcg
     1981 ctcatggggg aggacctgcg caacctgccg caggcattcg cccacgcgcg gtacgcccgc
     2041 gccatcatgc tgcagaacgt cgggttgtcc atcgccttga tcgcgatcct catcccgctc
     2101 gccctcgccg gtgtgctcgg tctggcggct gtggtcctgg tgcacgagct cgctgaggtg
     2161 gtcgtgatcg ccaacggcgt gcgcgctggt cgcgcgcaga ggctcctgat cggcggcacc
     2221 gcgagcgtcc cagagcctgc ggtcgccgcc gcgcgggtga gactcggtct ggagtcctag
     2281 cggacgaggt cagcacgaga acgaccagcg tcgctcgcgc ggctgatggg taccacctgg
     2341 tggaacgcca actcacgtgg tgctctgccc gctccggacg tggtcagctc gggcgcggcg
     2401 cgtacatgag gactgcgacg ccgacgaggc agatgagcgc accggcgatg tcccagcggt
     2461 ctggacggaa cccgtcgacg agcatccccc acgccaggga tccggcgatg aagactccgc
     2521 catatgcggc caggatgcgc ccgaagtcag cggacggctg cagggtgacg acaagcccgg
     2581 acagaccgag cgcaaccgcg ccggccccga cccagaccaa ccacgcacca ccgatctcga
     2641 ac
//
LOCUS       DASRKH010000199         3742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_14231, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000199 DASRKH010000000
VERSION     DASRKH010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14231"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..847)
                     /locus_tag="VFC06_03290"
     CDS             complement(197..847)
                     /locus_tag="VFC06_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80957.1"
                     /translation="MEATVDAMGPLVGGLDDTGLADVAGIYLVGGASGLPLVPRLLRE
                     RFGRRVHRSPYPAASTAIGLAIAADDAAGFSLVDRLSRGFGVFREASGGRALSFDPIL
                     SRDERVSATAEVVVTRHYRAAHNVGWYRFVEYSSVDERGEPRGDLVPFAEVVFPFDPA
                     LQQAGDLRAVEVERRERGPLVEERYTIDTHGIVEVQITDLVTGYSLTRSFSARDGG"
     gene            complement(958..1815)
                     /locus_tag="VFC06_03295"
                     /pseudo
     CDS             complement(958..1815)
                     /locus_tag="VFC06_03295"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
     gene            1844..3556
                     /locus_tag="VFC06_03300"
     CDS             1844..3556
                     /locus_tag="VFC06_03300"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HZL80958.1"
                     /translation="MASIVGKKRGNQTYYYLVESARVDGKPRIVEQQYLGSAEEVIAR
                     LSGAPAGSPERTQHKKFGDLAAVWGILGRLGVAEAIDAVCGSRRSDAGASVGTYLALA
                     TLNRIVAPCSKLAFEPWWATTAGPRFTKVPVAATDHRRFWDAMDALEPTKLAAAEAAI
                     STAMVTEFGLDLSGLALDMTNFATFIDSANEKAPIAQRGHAKQKRNDLRLVGLGLVVT
                     RDGAIPVTSHTYAGNRNDVSQFTTVLDELTSRHGALLDHQGGGGERQQPPTVVFDAGQ
                     NSAANFTHLAETGLHFVGSLPPSDFPDLLALPARRRRRVDPEKVPGLSAYDTRALVFG
                     TDRRVVLTHSTSLHAKQSAGFDQTLAKATRALNELAATLARGKTRRNRTAVLATISTI
                     TSPRWVNRVLTTDLTGTTPAKLRLTWHIDPAARKTLETELFGKRLLVTDHDDWTVSEV
                     VSGYRSQNDVESGFRQLKDPHVVSFSPMFHWTDSKIRVHVFYCVLALAVAHLMRREAH
                     HAGIDLSVRELLTTLGGIEETVLLYPTGNKGRPRAQRILTDQNPTQQRLYELFNLHTY
                     APRR"
BASE COUNT          654 a         1340 c         1232 g          516 t
ORIGIN      
        1 acagggcgtt gcgaccctcg ccggcgccga ggtccacccc gcgcccgggg ggcaactccg
       61 cgagttcggc cacgacgaac ccgttcggcg cggcggacca caccgtaccc ccggctcggt
      121 cagcttcgcg gtatcgcgcg tcccaggtct ggctcgtcac aggccgagta ttccaccacc
      181 gcgtcccggc cggctctcag ccgccgtccc gggcgctgaa ggaccgcgtc aacgagtacc
      241 ccgtcaccag gtcggtgatc tgcacctcga cgatgccgtg ggtgtcgatc gtgtacctct
      301 cctccaccag cgggccgcgc tcacggcgtt ccacctcgac ggcgcgcagg tcgccggcct
      361 gctgcagggc cgggtcgaac gggaagacga cctcggcgaa cggcacgagg tcgccgcgcg
      421 gctcaccccg ctcgtcaacc gaggagtact cgacgaaccg gtaccaaccc acgttgtgcg
      481 cggcgcggta gtgcctcgtg acgaccacct ccgctgtcgc cgagacccgc tcgtcacggc
      541 tcaggatcgg atcgaagctc agtgcccgcc cgcccgacgc ctcccggaac acgccgaacc
      601 cccgcgagag ccggtcgacg agcgagaagc ccgccgcgtc gtcggccgcg atggcgagcc
      661 cgatggcggt ggatgcggcc gggtaggggg agcggtggac gcgccgaccg aagcgctcac
      721 gcaggaggcg ggggaccagc ggcaggccgc tcgcgccacc caccaggtag atgcccgcga
      781 cgtcggcgag cccggtgtcg tcaagcccgc ccacgagcgg gcccatcgcg tccacggtgg
      841 cctccaccag gtgtgcggcg gcctcgtaga acccgacgac gggacggtca ccggcatgcc
      901 gtccacctcg agggcgacgc gcttggactg tggggccaga tgctccttgg cgtcgcggca
      961 ctcctcgagc agggacgcga gcgcgcgtgg ttcgaggtcc gcgcgcgtga tgccggccgc
     1021 gtccagagcg aggccggcga gtacggcgtc gaagtcgtcg ccacccaggt ggttgagccc
     1081 gatcgagtcg aggacctcgt ggctggtgcc gtccacgtgc acgagcgagg catcgaaggt
     1141 gccgcccccg aggtcgtaga cgaggacgcg ggtgcggcgg gagttcagcg tgcggccccg
     1201 gcggtgcgag tactcgaaac cggccgcgga cggctcgttg accatcgcgg tgacggtgaa
     1261 gcccgcccct ttgaaggctt caagcgtgag gaaccgctgc gcgccgtggg cgtgggcggg
     1321 gacggcgatc acggtccgcg tggtgtcgag ttcgccgagg ctggagcgcg taccgagggc
     1381 ggtcctgagt gcggttgcga agccggtgag cacgtcgagc agcgggatgt cctggtcacc
     1441 gatgcgcagg gtggtggcgg tgttgacgtc cggttcggcc agcgcccgct tgaaggaccg
     1501 cagcagggga gcgccgccgg cggcggcggc gagcgcgtcg aagccgtaca ggagggcgcc
     1561 acccgcaagg gcgacgaccg acgggaagta ctcgtgggcg tcaccgtcag gatcgaggaa
     1621 gctcacgacc gggtagttgc cccggtccac ggaggccacg atggtgcgag ttgtcccgaa
     1681 gtcgattccc actggcatgg cttcgagcct agcgggagtt tccggggtag ttcgatcgtg
     1741 agtggaccgg gtgaggttgg tactggctga agaccggtgt tgacctgcgc agatgcgcgt
     1801 gtcggtgctg gacatcaggg ctcggagtat gtattaccca tatatggcat cgatcgtggg
     1861 gaagaagcgc gggaaccaga cctactacta cctcgtggaa tcagcccggg tcgatggcaa
     1921 acctcggatc gttgaacagc agtacctggg cagcgccgag gaagtgatcg cccgactctc
     1981 cggtgccccg gccggcagcc cagaacgcac ccagcacaag aagttcggcg acctcgccgc
     2041 ggtctggggg atcctggggc gcctcggtgt ggccgaggcg atcgatgcgg tgtgcggatc
     2101 gcggcgttcg gacgcgggcg cgtcggtggg gacctacctg gccctggcca ccttgaaccg
     2161 gatcgtcgcg ccgtgcagca agctggcctt cgaaccctgg tgggccacca cagccggccc
     2221 acggttcacc aaggtgcccg tggcagcaac agaccaccgc cggttctggg acgcgatgga
     2281 cgccctggag cccaccaagc tcgccgccgc cgaagcggcg atcagcaccg cgatggtcac
     2341 cgagttcggt ctggacctgt cggggttggc gttggacatg acgaacttcg cgaccttcat
     2401 cgattcggcc aacgagaagg ccccgatcgc ccaacgtggc cacgccaagc agaaacgcaa
     2461 cgacctgcgc ctggttgggc tcgggctcgt ggtgacccgc gacggcgcga tcccggtcac
     2521 tagccacacc tatgccggga accgcaacga tgtcagccag ttcaccaccg tgctggacga
     2581 gctcacctcc cgccacggcg ccctgctcga tcaccaaggc ggcggcgggg aacgacagca
     2641 gccgccgacc gtggtcttcg acgccggaca gaactccgcc gcgaacttca cccacctggc
     2701 cgagacaggg ctgcacttcg tcgggtcgct accgcccagt gacttccccg acctactcgc
     2761 cctaccagcg cgccgacgcc gccgggtgga ccccgagaag gtcccggggc tgagcgccta
     2821 cgacacccgt gccctggtct tcggcaccga ccggcgggtc gtcctgactc actcgacgag
     2881 cctgcacgct aaacagtccg ccgggttcga ccagaccctg gccaaggcca cccgggcgct
     2941 gaacgagctc gcggccacgc tggcccgcgg caagacccgc cgcaaccgca ccgccgtcct
     3001 ggccacgatc agcacaatca ccagcccccg ctgggtcaac cgggtcctga ccaccgacct
     3061 gaccgggacc accccggcga agctgcggct gacctggcac atcgaccccg ccgcccggaa
     3121 aacactggaa accgaactgt tcggcaaacg cctcctggtc accgaccacg acgactggac
     3181 cgtctccgag gtcgtctcgg gctaccgctc ccagaacgac gtcgagtcag gattccggca
     3241 actcaaagac ccccacgtgg tcagcttctc cccaatgttc cactggactg actccaaaat
     3301 ccgggtccac gtcttctact gcgtcctcgc cctggccgtc gcgcacctga tgcgccgcga
     3361 agcccaccac gccggcatcg acctcagcgt ccgcgaactc ctcaccaccc tgggcgggat
     3421 cgaagaaacc gtcctgctct acccaaccgg caacaaaggc cgaccccgcg cacaacgcat
     3481 cctcaccgac cagaacccca cccaacagcg cctctacgaa ctcttcaacc ttcacaccta
     3541 cgcaccgcga cgctgacatg ggtaatacaa cccaccaggc cccacaccca cgctgacctg
     3601 cacatttacc actcgccgcc gcgatagccc ggaaactccc gctagggccg ccgaccgaca
     3661 caagagcgcg gtggctgagt acaatcgcgc cgttgagcgg agaaacgacc aactcaccga
     3721 ggtacagcgc aaatgcaagg tt
//
LOCUS       DASRKH010000200         2407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_32038, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000200 DASRKH010000000
VERSION     DASRKH010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32038"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..679
                     /locus_tag="VFC06_03305"
     CDS             215..679
                     /locus_tag="VFC06_03305"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HZL80959.1"
                     /translation="MDVSGQIAELAGPAAQSAGMIVDGVDVAKAGSRTRVLVTVDLPD
                     DAIGAAELDTVAEASRAIGAALDEAGVPAGAYVLEVSTPGTDRILTERRHFLRARTRL
                     VELDLTDGTETTGRLSDLVEDTLVLDNDGVEARIALADVTCGRVQVEFKKLK"
     gene            693..1682
                     /gene="nusA"
                     /locus_tag="VFC06_03310"
     CDS             693..1682
                     /gene="nusA"
                     /locus_tag="VFC06_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012877982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HZL80960.1"
                     /translation="MDIDMATLRGLEHERDLKLDVLIDAIEQALLSAYHKTPGALTHA
                     RAFVDRDSGQVTIHAREEGEDGELGPEFEHTPHDFGRIATATARQVIVQRLRQAGDDA
                     VLGQYADKEGTLVSGVIQQSADPRNVHVDVGDVEALLPGHEQVPTETYVHGERLRGYV
                     LTVARGAKGPSITLSRTHPGLVRKLFEMEVPEIADGTVEIMSLAREAGHRSKMAVRAT
                     VPGVNAKGACIGPMGARVRAVMSELRGEKIDIVDWDDDPATFVGNALSPSRVVSVTVV
                     DAEARSARVIVPDYHLSLAIGKEGQNARLAARLTGWRIDIRSDEAPEPQGDPN"
     gene            1766..2005
                     /locus_tag="VFC06_03315"
     CDS             1766..2005
                     /locus_tag="VFC06_03315"
                     /inference="COORDINATES: protein motif:HMM:NF016204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HZL80961.1"
                     /translation="MGCRGQDARSVLVRVVLTDGRLAVDESATAPGRGAWLHPDGVCL
                     SHATKRHAFGRAFRTAGFDASGLIEHPAFRDNHGP"
     gene            2123..>2407
                     /locus_tag="VFC06_03320"
     CDS             2123..>2407
                     /locus_tag="VFC06_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013398733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HZL80962.1"
                     /translation="MAKPRVHEIAKELGIPSKDLIAKLNELGEYVKGPSSTLEGPVVR
                     KAKEAFPAPTTPAVPEPASKPAASKPSPKPAAPKPATPEPEPEPQAPEPAA"
BASE COUNT          466 a          793 c          798 g          350 t
ORIGIN      
        1 gagtggggaa gggggagggg ccggtttccc aacgcacgcc acatgcggag acaagcatcg
       61 ccaccgccac ggccacagca acagccgtaa agcccctcag tcgtcgaccc gccatggcgg
      121 atagtctcgc acgcgctcgc cgggggccct agaatcatcg cgtaggtcaa aattcagcaa
      181 cgggcgtgcg gacagtacgc ggggaggcaa acgcatggat gtgagcggcc aaattgccga
      241 actcgcggga cccgcggcac agtccgccgg gatgattgtt gacggcgtcg acgtagccaa
      301 ggcgggctcg cgcacgcgag tgcttgtcac cgtggatctt ccggacgacg ccatcggcgc
      361 ggcagaactc gataccgtcg ccgaagcgtc ccgcgccatc ggcgcagcgc tcgacgaggc
      421 tggcgtgcca gcgggcgcct acgtgctcga ggtatcgacg ccgggcacgg accggatcct
      481 cacggagcgg cgccactttc tgcgcgcacg cacccgactg gtcgagttgg acctgaccga
      541 cggaaccgag accacaggac gactgagcga cctcgtcgag gacacgctcg tgcttgacaa
      601 cgacggcgtc gaggcgcgca tcgcgctggc cgacgtgacg tgtggccgcg tccaggtcga
      661 gttcaagaaa ctgaagtaga agcgagacac acgtggatat cgacatggcg acccttcggg
      721 gtctcgaaca tgagcgcgac ctcaaacttg acgtcctcat tgacgcgatc gagcaggcgc
      781 ttctgagcgc gtatcacaag acaccgggcg cgttgactca cgctcgggcc ttcgtggacc
      841 gcgactcggg ccaagtcacc attcacgccc gcgaggaggg ggaggacggc gagctcggcc
      901 ccgagttcga gcacactccg cacgacttcg ggcggatcgc gaccgcgacc gcgcggcaag
      961 tgattgtgca gcggcttcgc caagcgggag acgacgccgt cctgggtcag tacgcggaca
     1021 aggaaggcac actcgtttca ggcgtgatcc agcaaagcgc cgacccccgc aacgtgcatg
     1081 tggatgtggg cgacgtcgaa gcgctgctgc cgggccacga gcaggtgccg acggaaacgt
     1141 acgtgcacgg cgagcgcctc cgcggctacg tgctgacggt cgcacgcggc gcgaagggac
     1201 cctccatcac cctgtcccgc acccacccag ggctcgtgcg caagctgttc gaaatggaag
     1261 tgcccgagat cgcagacggc accgtggaga tcatgagtct ggcccgcgag gctggccacc
     1321 gctccaagat ggccgtccgc gcgaccgtgc cgggcgtcaa cgccaagggc gcgtgcatcg
     1381 gcccgatggg cgcccgagtg cgtgccgtca tgagcgaatt gcgcggcgaa aagatcgaca
     1441 tcgtcgattg ggacgatgac ccggccacgt tcgtcggcaa cgcattgtcg ccgtcgcgag
     1501 tggtgtcggt gacggtagtg gacgcagagg cccgttccgc tcgcgtgatc gtgccggact
     1561 atcacttgtc gctcgcgatc ggcaaagagg gccagaatgc gcggctcgcc gcacggctca
     1621 ctggctggcg tattgatatc cgatctgacg aagcgccgga gccccaaggt gacccgaact
     1681 gaccgagcgc ggtagactag acgcgccgaa cgtcgagcca cgctctgacg cgcgccccac
     1741 aggacacgtt cctgtgcgaa cgtgcgtggg atgtcgaggg caagacgctc ggtcggttct
     1801 tgtgcgagtg gtgctgaccg acggccgtct tgcggtcgac gaatcagcga ccgcgcctgg
     1861 tcgtggagcc tggctccatc cggacggggt ttgcctctcg cacgcgacga agcgccacgc
     1921 ttttggccgc gcatttcgca cggcaggttt cgacgcgtcg gggctcatcg agcatccggc
     1981 gttccgcgac aaccacggac cgtagcccac ccatcgaccg gacaggatga aagccaatgg
     2041 tcatccaatg agtacccgta aatgagtacc ccgcagtaac gatggttcga gcctgtcttg
     2101 gtcggaccga gacaggagaa ttgtggctaa gccccgcgtt catgagatcg cgaaggagct
     2161 cggtattccg agcaaagacc tcattgcaaa actgaacgag ctcggcgaat atgtcaaggg
     2221 tccctcgtcg accctcgaag ggcccgtcgt gcgtaaggcc aaagaggcct tccccgcgcc
     2281 gacgacccca gccgttccgg agcctgcgag caagcccgca gcgtccaagc cgtcccccaa
     2341 gcccgccgcg cccaagccgg cgacccccga acccgaaccg gagccgcagg cccccgagcc
     2401 cgcagcc
//
LOCUS       DASRKH010000201         2141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_16963, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000201 DASRKH010000000
VERSION     DASRKH010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16963"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VFC06_03325"
     CDS             complement(<1..232)
                     /locus_tag="VFC06_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80963.1"
                     /translation="MTAEDPTPQPENPDVAAGTPADAADALDYVVLTTPEGHADGVVR
                     CQRCGATEVEYSASKGNTALMMLLVEVVEWMST"
     gene            complement(229..1410)
                     /locus_tag="VFC06_03330"
     CDS             complement(229..1410)
                     /locus_tag="VFC06_03330"
                     /inference="COORDINATES: protein motif:HMM:NF024813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HZL80964.1"
                     /translation="MGFIQITVGAAWDSLKSSFGDQFLDFLVPPQGLTDTAAVFPAVR
                     QATNAGRGRNTAASENIISNGSVIVVPEGYGLLTFQDGALTSVVAESGAFKWDSEDQN
                     SKSVFAGDGIFSSTFKTSWERFKFGGRPGSQQLAFFVNLKEIPNNKFGTQGPLYWDDA
                     YLNAQAGAVTRGTYVLKIVDPVLFIKNVVPASYITAEGKVLDLDEPDSDVAEQLFTEV
                     VGSLAGAFSRYVNDDDKGHRITKIQSDSVGFARSLADEVETNYQWGTGRGLEITKAAI
                     VSIEYDEDTTALLSDVRKADALSGGRADTFLKQSVARGVEAAGESGGGGTGLAMMGMG
                     MGAIGNLVQPTPQAPAAAGTGRPDGAAGAVQADARRRPHHAGGLRRVEEEGPGPVGGD
                     S"
     gene            1612..2040
                     /locus_tag="VFC06_03335"
     CDS             1612..2040
                     /locus_tag="VFC06_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HZL80965.1"
                     /translation="MPAIPSDRAAHFIVSAVVLRDRRGRIVTVRKAGTGRFMLPGGKP
                     EPGESAAQAAIRECAEELGLELNPEHLTVLGVFTADAANEPGLTLEGSVFEHPLPGEP
                     HAAAEIAELRWLDPALPLPDDLAPLLEHHVLPMLAAATGP"
BASE COUNT          328 a          730 c          713 g          370 t
ORIGIN      
        1 aggtggacat ccattcgacc acctcaacca acaacatcat caacgcggtg ttgcccttcg
       61 aagcggagta ttccacctcg gtggctccgc accgttggca gcgcaccacg ccatcggcgt
      121 gcccctcggg cgtggtgagc accacgtagt cgagcgcgtc ggccgcgtcg gcaggcgtgc
      181 ctgcggcgac gtcggggttc tctggttgcg gcgtgggatc ctcggcggtc atgagtcccc
      241 gcctacaggc ccaggacctt cttcttcaac gcgtcgaagt cctcctgcgt gatgaggccg
      301 tcgtcgagca tctgcttgta ctgcaccagc tgcgccgtcg ggtctgccgg tgcctgcggc
      361 agcgggcgcc tgaggggtgg gctggaccag gttgccgatg gcgcccatgc ccatgcccat
      421 catcgccaag ccggttccgc cgccacccga ttcccccgcg gcctcgacgc cgcgcgccac
      481 agactgcttg aggaacgtgt cggcacggcc cccggacagg gcgtccgcct tccggacgtc
      541 ggacagcagt gccgtggtgt cctcgtcgta ctcgatggag acgatggcgg ccttggtgat
      601 ctccaggcct cggccggtgc cccactggta gttggtttcc acttcgtcgg cgagcgaacg
      661 cgcgaagccg acagagtcgg actggatctt ggtgatccgg tggcccttgt cgtcgtcgtt
      721 cacgtagcgc gagaacgcgc cggcgagcga gccgaccact tcggtgaaga gctgctcggc
      781 gacgtcgctg tcgggctcat ccaggtcgag gaccttcccc tcggcggtga tgtagctggc
      841 gggcaccacg ttcttgatga acagcaccgg gtccacgatc ttcagcacgt acgtgccgcg
      901 cgtcactgcg cccgcttggg cgttgaggta ggcgtcatcc cagtagaggg ggccctgcgt
      961 gccgaacttg ttgttcggaa tctccttgag gttcacaaag aacgcgagct gctggctgcc
     1021 cgggcgaccg ccaaacttga agcgctccca cgaggtcttg aaggtggagc tgaagatgcc
     1081 gtcgcccgcg aacacggact tggagttctg gtcctcgctg tcccacttga aggcgcccga
     1141 ctcggccacc acgctcgtga gggcaccatc ctggaaagtg agcagtccgt aaccctccgg
     1201 cacgacgatc acggagccgt tggagatgat gttctcgctg gctgcggtgt tgcgtccacg
     1261 gcccgcgttg gtggcctgcc gaactgcggg gaacacggcc gccgtgtcgg tgaggccttg
     1321 gggtggcacc aggaagtcga ggaactggtc gccgaatgag gacttcaggg agtcccacgc
     1381 ggctccgacg gtgatctgga tgaaccccat ggtcgctcct gtctgccgcc gcggtctccc
     1441 gcacggcatc gcccactcat agagcccgca gcgcccggcg cccgtcgcct gacatcggcg
     1501 aaccctcgtt cggcaatcta gcaggaacgg gtccacagga ccacggttgc cggggctcgc
     1561 gccgggcgaa tgccgggacg attcatcggt atgccgtggc accatgaccc catgcctgct
     1621 atccccagtg atcgcgccgc ccacttcatt gtcagcgccg tggtgcttcg cgatcgccga
     1681 ggacgcatcg tcaccgtccg caaggcgggc acaggccgct tcatgctgcc cggcggcaag
     1741 cccgaacctg gcgagtccgc cgcgcaggcc gcgatcaggg agtgcgccga ggagctcggc
     1801 ctcgaactca accccgagca cctgaccgtg ctgggcgtct tcacggccga tgccgctaac
     1861 gagcctgggc tgaccctgga gggctccgtg ttcgagcacc cgctccccgg agaaccgcac
     1921 gccgccgccg agatcgcaga gctgagatgg ctcgacccgg cgctgccgct gcctgacgac
     1981 ctcgcgccgc tgctggagca ccacgtgctg ccgatgcttg cggcggcgac cggaccctag
     2041 ccaggtgcgg gcgatctcgc tcaggcgacg atgtcatacc tggcccttcc gccgatgcgc
     2101 ggagtgcggg tggggtcgac agatcgcggc ggggtgaacc a
//
LOCUS       DASRKH010000202         2191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_58790, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000202 DASRKH010000000
VERSION     DASRKH010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58790"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..145)
                     /locus_tag="VFC06_03340"
     CDS             complement(<1..145)
                     /locus_tag="VFC06_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HZL80966.1"
                     /translation="MIVVTRLNGPQFGVNPDLLQRIDSAPDTILTLIDGTKYIVEESM
                     TEVI"
     gene            210..386
                     /locus_tag="VFC06_03345"
     CDS             210..386
                     /locus_tag="VFC06_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80967.1"
                     /translation="MTCTREHSSATKHTHLAPATTVGAGRTSAAAAPARCGAAAADEG
                     SEVGDAGGVADVSA"
     gene            complement(376..1554)
                     /locus_tag="VFC06_03350"
     CDS             complement(376..1554)
                     /locus_tag="VFC06_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HZL80968.1"
                     /translation="MLRSLFSGISGLRSHQTMLDVTGNNIANVNTTGFKSSQTQFQDA
                     LSQVVQNASGAQAGTGGTNPAQVGLGVRVAGVTTNFTQGASQLTGRSTDMMIQGDGFF
                     VVRKGADTFYTRAGGFDFDATGQMVLPGEGALVQGWAAVNGVIDTNSPLTDLRVPAGT
                     LMGAIATTSATYEGNLQSDAATGTTIERAVKAYDSTGVEREITLTFTKSAAGWSLAAA
                     DGTSTAAPGAMTFAADGSLTSPTGFTLGSVSVDLSTVTGFAGVQTAAASTQDGHSAGT
                     LQAFSLGADGTITGSFSNGLKQTVGRIALGNFTNPAGLEKAGGSLFRTTVNSGNAQVG
                     AAGTGGRGGLAGGALEMSNVDLSSEFTNLIIAQRGFQANSRVITTSDELLQELVNLKR
                     "
     gene            complement(1583..2059)
                     /locus_tag="VFC06_03355"
     CDS             complement(1583..2059)
                     /locus_tag="VFC06_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HZL80969.1"
                     /translation="MSIDVTPITWGSAAAAASTGTATASTAAAPGTLDKEAFLKLLVA
                     QLRNQDPSKPMDSSELMAQTTQLSTMEQLTALTKTSQESFALQMRMAAASLVGRQVTY
                     ADESGATVTGAVTSVSYAGSVPTVTVGGKVVALDAVSSVTAPATATPAAPASSTTV"
BASE COUNT          295 a          734 c          816 g          346 t
ORIGIN      
        1 cgatcacctc ggtcatcgac tcctcgacga tgtacttcgt gccgtcgatg agcgtgagga
       61 tggtgtctgg cgcactgtcg atgcgctgca gcaggtcggg gttcaccccg aactgcggcc
      121 cgttgaggcg cgtcacgacg atcacagcga gtccgtccct ggtacgactt caggcccatc
      181 cgtgggcctt gtacccactg gatcggctga tgacctgcac tcgtgagcat tcctcggcca
      241 ccaagcacac ccatctcgca cccgcgacca cggtcggggc cggacgcacg tcggccgcgg
      301 cggccccggc gaggtgcggg gccgccgcgg ccgacgaggg cagcgaggtc ggggacgccg
      361 gcggcgtcgc cgacgtcagc gcttgaggtt gacgagctcc tggagcagct cgtcggacgt
      421 cgtgatgacg cgcgagttcg cctggaaacc gcgctgggcg atgatcaggt tggtgaactc
      481 gctcgacagg tccacgttgg acatctcgag cgcgccgccc gcgagcccgc cgcgaccgcc
      541 cgtgccggcg gcgccgacct gcgcgttgcc cgagttgacc gtcgtgcgga acagcgaccc
      601 gccggccttc tcgagcccgg ccgggttcgt gaagttgccg agcgcgatcc ggccgacggt
      661 ctgcttgagg ccgttgctga acgagcccgt gatggtgccg tcggcgccga gggagaacgc
      721 ctgcagcgtg ccggccgagt ggccgtcctg cgtcgacgcc gcggcggtct gcacaccggc
      781 gaagcccgtg actgtggaca ggtcgaccga caccgaaccg agcgtgaagc cggtcggcga
      841 cgtgagcgat ccatcggcag cgaacgtcat cgcgcccggc gccgccgtcg aggtcccgtc
      901 ggccgcggcg agcgaccagc cggcagcgga cttggtgaac gtcagcgtga tctcgcgctc
      961 gacgcccgtc gagtcgtacg ccttcacggc ccgctcgatg gtcgttccgg tcgcggcgtc
     1021 ggactgcagg ttgccctcgt aggtcgccga cgtcgtcgcg atcgcgccca tgagcgtgcc
     1081 ggccgggacc cgcaggtcgg tcagcgggct gttcgtgtcg atgacgccgt tcacggccgc
     1141 ccagccctgc acgagagcgc cctcaccggg caggaccatc tggccggtcg cgtcgaagtc
     1201 gaagccgccc gcccgcgtgt agaacgtgtc ggcacccttg cggaccacga agaagccgtc
     1261 gccctggatc atcatgtccg tgctgcggcc cgtgagctgc gaagcgccct gcgtgaagtt
     1321 cgtggtcacg cccgcgacgc gcaccccgag accgacctgc gcggggttcg tgccacccgt
     1381 gccggcctgg gcgccgctgg cgttctgcac gacctggctc agcgcgtcct ggaactgcgt
     1441 ctgggaggac ttgaagcccg tcgtgttgac gttggcgatg ttgttgccgg tcacgtcgag
     1501 catggtctgg tgactgcgca gaccgctgat gccggagaag agcgagcgga gcatggggag
     1561 atcccttcgg tggtggactg cgtcagacgg tcgtggagga tgcgggtgct gcgggggttg
     1621 cggtggctgg tgcggtgacc gacgacacgg cgtcgagcgc gacgaccttg ccgccgacgg
     1681 tcacggtggg gacggacccg gcgtacgaga cggacgtcac cgcgccggtg acggtcgccc
     1741 cggactcgtc ggcgtaggtg acttgccggc cgacgagcga cgccgcggcc atgcgcatct
     1801 gcagcgcgaa gctctcctgc gacgtcttgg tcagcgcggt gagctgctcc atcgtggaca
     1861 gctgcgtggt ctgggccatg agctccgagc tgtccatcgg cttgctcggg tcctggttgc
     1921 gcagctgcgc gacgaggagc ttgaggaacg cctccttgtc gagcgtgccc ggggccgccg
     1981 ctgtggaggc ggtggcggtg cccgtcgagg ccgccgcggc cgccgatccc caggtgatgg
     2041 gtgtgacgtc gatgctcatg ggtgctccgt gatcagacga gaaggtcgag gacgccgggc
     2101 cgagcggccg agcgggtggg atcggggact gcggtgcggc gcgccggtcc cgcgctgtcg
     2161 gcgtccccgc gcggcgccga gcgacgacct g
//
LOCUS       DASRKH010000203         2068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_40116, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000203 DASRKH010000000
VERSION     DASRKH010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40116"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VFC06_03360"
     CDS             complement(<1..516)
                     /locus_tag="VFC06_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome C reductase"
                     /protein_id="HZL80970.1"
                     /translation="MSEKHITSGETAGRANGFENPGLEPHRPRMSDTDPRAAKRAEMQ
                     VVGLLVVSIVGAIGGVAGFFLFPAGESLGTLRLGNTALGLGLGLGLLGIGLAAVHWAK
                     SLMNDTEQVEARHDLSSDATTRAAAVANLEAGVKDANIARRPLLKGALASALALAPLP
                     FVLPLIGNLTPD"
     gene            complement(513..1289)
                     /locus_tag="VFC06_03365"
     CDS             complement(513..1289)
                     /locus_tag="VFC06_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZL80971.1"
                     /translation="MNALTSARRSRHAPALLVLLALLLTGGLYALMSPAADAEVGSAS
                     DIDGGQKLFQANCSTCHGLNAEGNDIGPSLIGVGAASVDFQVSTGRMPMASNSVQAPR
                     KEPMFDAQQTSMLAAYVASLGAGPGIPSAEAVDPTLGNPANGMQLFRTNCAMCHNAVG
                     AGGALSEGKYAPPLYKATPTQIYTAMLTGPQAMPVFNEASITPEGKRDIIAYLMEQRE
                     TAPGGLTLGSLGPVSEGLWAWVLGLGSLIGIAVWIGAKSS"
     gene            complement(1309..1941)
                     /locus_tag="VFC06_03370"
     CDS             complement(1309..1941)
                     /locus_tag="VFC06_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HZL80972.1"
                     /translation="MSSHSAVAPSVHVHVDRPNPVSVGTIVWLASELMFFAGLFAMYF
                     THRSVNYDQWPGGTELLNLPFSFANTTVLVLSSVTCQMGVWAAERFRPRRTGSLLQVG
                     QWGVVEWFTLTYVMGAVFVAGQIYEYAELFSVGLDVSTSYGSVFFITTGFHGLHVVGG
                     LIAFLFVLGRTFTAKRFGHHEATTAIVTSYYWHFVDVVWIALFTTIYLIR"
BASE COUNT          339 a          658 c          757 g          314 t
ORIGIN      
        1 gtccggggtg aggttgccga tgagtgggag gacgaacggc agtggcgcca gtgccagggc
       61 cgaggcgagg gcgcccttga gcaggggacg ccgggcgatg ttcgcgtcct tgacgccggc
      121 ctcgaggttg gccaccgctg cggcgcgcgt cgtggcgtcg ctggaaaggt cgtggcgagc
      181 ctccacctgc tcggtgtcgt tcatcagcga cttcgcccag tgcaccgcgg cgagcccgat
      241 gcccagcagc ccgagcccga ggcccagccc gagggcggtg tttccgaggc ggagggtccc
      301 gagcgactcg ccggcgggga acaggaagaa cccggcgaca ccgccgatgg cgccgacgat
      361 cgacacgacg agaaggccga ccacctgcat ctcggcccgc ttcgccgccc gtggatccgt
      421 gtccgacatg cgcgggcggt gcggctccag cccggggttc tcgaatccgt tggcccggcc
      481 ggcggtttcg ccgctcgtga tgtgcttctc gctcacgacg acttcgctcc gatccacacg
      541 gcgattccga tcagcgagcc gagtccgagg acccacgccc acaaaccctc gctcacggga
      601 ccgagggagc cgagggtgag gcccccgggt gcggtctccc gctgctccat gaggtaggcg
      661 atgatgtcgc gcttgccctc cggggtgatg gaggcctcgt tgaagaccgg catcgcctgg
      721 gggccggtga gcatggccgt gtagatctgg gtgggggttg ccttgtacag cgggggagcg
      781 tacttgccct cgctcagggc gccgccggcg ccgacggcgt tgtggcacat tgcgcagttg
      841 gtccggaaga gctgcatgcc gttcgccggg ttcccgagcg tggggtccac cgcctcggcg
      901 gacgggatcc ccgggccggc gccgagcgag gcgacgtagg cggcgagcat gctggtctgc
      961 tgggcgtcga acatcggctc cttgcggggg gcctgcacgg agttggatgc catcggcatc
     1021 cggccggtgc tgacctggaa gtccaccgac gcggcgccca ccccgatgag ggacggaccg
     1081 atgtcgttgc cctcggcgtt caggccgtga caggtcgagc agttcgcctg gaagagcttc
     1141 tggccgccgt cgatgtccga cgcggagccg acctcggcgt ccgcggcggg actcatcaac
     1201 gcatacaggc cgccggtcag caacagcgcc aggaggacga gcagcgccgg ggcgtggcgg
     1261 ctcctcctcg cggaggtgag ggcattcatg agtggcgtgt cctttgcgtc agcggatgag
     1321 gtagatcgtg gtgaagagtg cgatccagac gacgtccacg aagtgccagt agtaggacgt
     1381 cacgatcgcg gtcgtcgcct cgtggtgccc gaaccgcttc gcggtgaagg tccggccgag
     1441 cacgaagagg aaggcgatga gtccgccgac cacgtgcagg ccgtggaacc cggtggtgat
     1501 gaagaagacg gaaccatagg acgtggacac gtccagtccc acggagaaca gctcggcgta
     1561 ctcgtagatc tggccggcga cgaagacggc gcccatgaca tacgtgagcg tgaaccactc
     1621 gacgaccccc cactggccga cctggagcaa tgagcccgtg cggcggggcc ggaagcgctc
     1681 cgccgcccac acccccatct ggcaggtcac ggaggagagc acgaggacgg tggtgttggc
     1741 gaaggagaac gggagattca gcagttcggt tccgcccggc cactggtcgt agttcaccga
     1801 ccgatgcgtg aagtacatgg cgaacaggcc ggcgaagaac atgagctcgc tggcgagcca
     1861 gacgatggtc ccgaccgaca cggggttcgg gcggtccacg tgcacgtgga cggagggggc
     1921 tacggctgaa tgcgacgaca cgctgtcatt attgcgtatc cgtgtgcgtt gtcaccttct
     1981 gagtgggcca ttggtcccga attccacgga tcgaatttcc cggcggccct ccgcggaccc
     2041 gaggccaccg aaaccggcct ccgcgggc
//
LOCUS       DASRKH010000204         2796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_24096, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000204 DASRKH010000000
VERSION     DASRKH010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24096"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(249..977)
                     /locus_tag="VFC06_03375"
     CDS             complement(249..977)
                     /locus_tag="VFC06_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80973.1"
                     /translation="MDDNMKQKILEQIEEQHLEPTPGWRFRLEHALKHFAVAFLFAVT
                     ALGLAGSVYILVQNGTLDDLRLGPRWAHPAFFDFPWQLLVLVVVLGILSFVVLRRTAR
                     LYRVARWWLVAALIVAVVAGAGAAYATNLTRFTFRAGPGQELFRRGGNPFGGMMGGAV
                     IGVVVSRTTQRWVVDSLTGERWLVVVTEETYFPSGSDIPVGTVVRVVGRHQKTTIYAV
                     GIRPVLGMDDMMGPGGMIIIPGTP"
     gene            complement(1027..1599)
                     /locus_tag="VFC06_03380"
     CDS             complement(1027..1599)
                     /locus_tag="VFC06_03380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZL80974.1"
                     /translation="MRAQEPTDEQLVRQALTDTDAFGMLVERYERPLSSYAGRLIGRQ
                     EAEDVVQQAFLQAYRSLAGFDPHLTFSAWMYRIVHNTAVNMARKRHGTVPTELELDGE
                     MVEILTGDTDVPAEALHRELEDFVAKGLNQLDQRSRDILLLTVVEGKSYSEIADILMV
                     AVNSVGPSVSRAKDKLRKLLDANPAQRSAL"
     gene            1858..>2796
                     /locus_tag="VFC06_03385"
     CDS             1858..>2796
                     /locus_tag="VFC06_03385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper ion binding protein"
                     /protein_id="HZL80975.1"
                     /translation="MKTKDLGITGMTCAACAIAVERSVKKLDGVASATVNSATDKLTV
                     EYDETRVDDGALKASIEKAGYGVAQPSAAKTVVIPIRGMTCASCSAANERALRKLAGV
                     TSASVNLTTEKATVVYDPTLVRVSAMKNAITAAGYTPLDVEVTAANVDEDAQRKAHET
                     RSLWQRFIVSALFAAPLLYLAMAPMVPWLAWSIPKWLSPMNVPLRYALVEIALVIPSI
                     VAGYWFYIVGFRAIWHLAPNMDSLIAMGTSAAILYSGYSTWRIAQGSVGAVKELYFET
                     AGVIITLILLGRSLESVSKGRTSQAIKKLMNLAPRTA"
BASE COUNT          529 a          901 c          835 g          531 t
ORIGIN      
        1 gccgagtgac atgcgtcacc tcccatgcgg tggtatccct tacagcatga gcaggtttgg
       61 ctcgaacgca accctcctgt ttggggttct tctttgagat gcgctgctca ttccgcccga
      121 gtatgttttt ccaatacccc cgaacatgtt agtccctcgc atccttttgt ccaagatgcg
      181 aggtgttctt caatgcgagg ggtgttctga ggggtgtact gaggggtgca tctacaggtg
      241 gcgttctcct acggtgtgcc ggggatgatg atcatgccac cgggacccat catgtcgtcc
      301 atgccgagca cggggcgaat gccgaccgca tagatcgtgg ttttctggtg ccgcccgacg
      361 acccggacaa ccgtccccac ggggatgtcc gaaccggagg ggaaataggt ctcctcggtg
      421 acgacaacca gccagcgctc gccggtaagg ctgtcgacga cccaccgctg ggtcgtgcgc
      481 gagacgacga cgccgataac ggcaccaccc atcatcccgc cgaaggggtt tcctccacgt
      541 cggaacagct cctgtcccgg cccggcgcgg aaggtgaacc gcgtgaggtt cgtcgcgtag
      601 gcggcgcctg ccccggccac gaccgcgacg atgagcgcgg cgaccagcca ccaccgtgca
      661 acacgataca ggcgggctgt gcgtcgcagg acgacgaacg agaggatacc gaggacgacg
      721 acgaggacca ggagctgcca ggggaagtcg aagaaggccg gatgcgccca ccgcggacca
      781 agccgcaggt cgtcgagcgt gccattctgg acgaggatgt acacactgcc cgccaatccc
      841 aaggcggtca cagcaaacag aaacgccacg gcgaaatgct tcagggcgtg ctcgagccgg
      901 aaccgccagc ccggcgtggg ttcgaggtgc tgctcctcga tctgctcaag tatcttctgc
      961 ttcatattgt cgtccatact catcctctct taattgtgaa gaccatcgtg ccaggcacca
     1021 aggtcttcac aatgcggacc gttgtgccgg atttgcatcc agcagtttcc gcagtttgtc
     1081 tttggcccga ctcacgctgg gtcccaccga gttgaccgcg accatcagaa tatccgctat
     1141 ctcgctgtaa ctcttgcctt cgaccaccgt cagtagcagg atatctctgc tgcgctggtc
     1201 cagctggttg agaccctttg ccacgaaatc ttccagctcc cggtgcagtg cctcggctgg
     1261 gacgtccgta tcgccggtca ggatctcgac catttcgcca tccagctcca gctcggtagg
     1321 cacagttccg tgccgcttgc gtgccatgtt caccgccgta ttgtggacga tgcggtacat
     1381 ccaagcactg aacgtcaggt gtgggtcgaa tccggcaagg ctgcggtacg cctgcagaaa
     1441 cgcctgctgg accacgtctt ccgcttcctg ccgcccgatg aggcggcccg cgtatgacga
     1501 caagggtcgc tcataccgct cgaccagcat tccaaaggcg tccgtgtccg tgagcgcctg
     1561 tcgaaccagc tgctcgtccg tcggctcctg agcgcgcatc tccatacata tatgatacca
     1621 aggaatgtcc ggttccgtac acgcaatatt gcggagaggc ggcaggcatg cccccgtacg
     1681 acactccgac tgctcagcga cgtcacacga ccgcgttgcg acaacatgcc gggcgttcac
     1741 aattgactcg ttgcagattc cggtgacccg gataggattg aggtacaccc ctgggggggt
     1801 ggggtactga ccgacgttga acatgctgcc actcgtggcg gcgaccggga ggaacagatg
     1861 aagacaaaag accttggaat cactgggatg acctgcgcgg cgtgtgcaat agccgtcgag
     1921 cgctccgtga agaagctgga cggcgttgcc tcggctacag tcaactctgc cactgacaag
     1981 ctgaccgtcg agtatgacga gacccgcgtc gacgacggag cgttgaaagc ctccatcgag
     2041 aaggccgggt atggcgtcgc ccagccgtcc gctgccaaga ccgttgtgat cccgatacgc
     2101 ggcatgacgt gcgcatcgtg ctccgccgcc aatgagcgcg cactccgcaa gctcgcgggc
     2161 gtgaccagtg cctcagtcaa cctgaccacg gagaaggcga ccgtcgtgta tgatcccacc
     2221 ctggtccgtg tgtccgctat gaaaaatgcc atcaccgcag ccggctacac gccgctcgac
     2281 gtggaagtca ccgcggcgaa cgtcgacgag gacgcgcaac gcaaggcgca cgagacccgc
     2341 tctctgtggc agcgcttcat cgtctccgct ctgttcgcgg cgcccctgct gtacctggcg
     2401 atggcaccca tggtgccgtg gctcgcctgg tccatcccga agtggctcag ccccatgaac
     2461 gttccccttc gctacgcgct cgtggagatc gccctggtca tccccagcat cgtggcgggc
     2521 tactggttct acatcgtcgg gttccgcgcc atctggcacc tggccccgaa catggactca
     2581 ctcatcgcga tgggcacgtc ggcggccatc ctctacagcg gctactcgac atggcgcatc
     2641 gcgcagggtt cggtcggcgc cgtgaaggag ctgtacttcg agaccgccgg cgtcatcatc
     2701 acgctcatcc tgttgggaag gtcgctggaa tccgtctcca aggggcgcac gtcgcaggcg
     2761 atcaagaagc tgatgaacct tgcgcccagg actgcc
//
LOCUS       DASRKH010000205         2038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_13729, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000205 DASRKH010000000
VERSION     DASRKH010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13729"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..240
                     /locus_tag="VFC06_03390"
     CDS             106..240
                     /locus_tag="VFC06_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80976.1"
                     /translation="MAGADARTAKRQDREAVRTGDADLAEYNAMLGGLADSDAPPRDP
                     "
     gene            1511..>2038
                     /locus_tag="VFC06_03395"
     CDS             1511..>2038
                     /locus_tag="VFC06_03395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZL80977.1"
                     /translation="MGDPKGDGDLPEDDAVAARLARLETILQLAPVGIGLVDFEGRTT
                     MTNSALHRMLGFTAQEWMADPGLWLRQLDPSDRDEALADKQRMLLAGTEAEARSDTYR
                     LLRRDGTTVWVRDDAMVLYDQDGTPTFQGVLVDVTQEKDLEERLGHQAFHDPLTGLPN
                     RRLFRERVDHALAGRA"
BASE COUNT          353 a          606 c          709 g          370 t
ORIGIN      
        1 ggattggggc gccgatgcga ttactgacca gcagtatggc ggctccattg cgtggggcct
       61 cggcgagttt ccagtgctgg tgttggccat cggcgtgctc gtggcgtggc gggggccgat
      121 gcccgtacgg ccaagcgtca ggatcgggag gcggtgcgca ccggggacgc agacctcgcg
      181 gagtacaacg cgatgttggg cgggctcgcc gactcggatg ccccgccgcg cgatccgtaa
      241 cgggcgggca ctgcatgacc gcggctcact ttggcggcga tgcctatcca cagattttgt
      301 ccacaggcat gtgagcgaag tggaaagtcc ctgcaaaggc cggtggataa agctgtgtac
      361 agggcgtgga ggaaaatcga ggtccgtgcg ccttgcgctc gcgggcacgg gtccgctaat
      421 actggggcat cgggaaaatc actactcgtt cagccggggt ttcgacgccg gtgatccgta
      481 gtcagtgccg atgctgggga actggtgtcg tggtggctgc ggggcgtgtg ggggtggact
      541 cgggcgcttc ggacaacggt ggtccaaggt gtgaggcagg gctcaggcct cgcgtcacac
      601 actgtgggtg acggcacagg gaccggcggc tcctcgcgtc gaccaatagg tcgttttcct
      661 cggaagacga agccgtatcg tcaatcacgg ctgtcggtag cgccccacgg ggcgatgtcg
      721 agaacttgag gtcatggcag ggcccctcga acgcttactt cgaggggccc ttcgcctgtc
      781 cccagccgca gctcggtgaa tgcccgtttc gtcgccagcg caccctcgct cgcggctctc
      841 gcgccgggta cttctccaca gattttgtcc acaggccggt ggattgtcga atgccagacg
      901 ggaaaatcgg tggaaaaacc tgtgcaataa atgtggaaaa agaaaacctc gatttcctgt
      961 tgcgctcccc aaattcgagg agtagacatt gcttattcgg atcgcacgaa gaggaacgga
     1021 gtggttgggg caacctggcc gcagcgggaa tctcgtcgat gcgaagtggt tcacaggatg
     1081 ttgcgctagc cctaagggat ctcctcggag agaacagtgg gaccgccggt aacggggtgg
     1141 tagcgacggg ccgtgaatcg cagagtgagg agggtcccgc ttgcggggtt cgccgattgg
     1201 gttcgttacc cggcggctgc ggtcggcgct tgcgtcggtc gtgatcctcg tctcaacagc
     1261 aaggcccccc ggacgcttac tccggggggc ctttgctgtg agacgaggcg tacggactgc
     1321 ctcgttcacc acaacgttat tgccgtagcc cgccgtaccg ctgaagcgac acgcgcggtc
     1381 ttcgaaatcg cccgtcggtc atgcccgcga cgttctctcc gggaggccga gtgacccgga
     1441 agtccgtatt gttcaaatat gggtcccggc ggccgatggg acagtgagtt cttagcagag
     1501 gaggtgggtc atgggtgacc ccaagggcga cggcgacctg cctgaggacg acgccgtggc
     1561 ggcgcgcctg gcgcggctcg agaccatcct ccagctcgcc ccggttggca ttggcctcgt
     1621 cgacttcgag ggtcgcacga ccatgacgaa cagcgcgctg caccggatgc tcggattcac
     1681 ggcccaggag tggatggcgg atcccggcct gtggctgcgc cagctcgacc cgtccgaccg
     1741 cgacgaggcg ttggcggaca agcaacgcat gctccttgcc ggaaccgagg cggaggcgcg
     1801 ctcggatacc taccggctcc ttcgccggga cggtacgacg gtatgggtac gcgacgacgc
     1861 catggtgctc tacgaccagg acggcacccc aacgttccaa ggcgtgctcg tggatgtcac
     1921 ccaggagaag gacctcgagg aacgcctcgg tcatcaggcc ttccacgacc cgctcaccgg
     1981 cctgccgaac cgcaggcttt ttcgcgagcg agtcgaccac gcgctcgctg gtcgggcc
//
LOCUS       DASRKH010000206         2257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_18035, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000206 DASRKH010000000
VERSION     DASRKH010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18035"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..154)
                     /locus_tag="VFC06_03400"
     CDS             complement(<1..154)
                     /locus_tag="VFC06_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZL80978.1"
                     /translation="MGDTRVILEMRHIIKEFPGVRALDDVTMSVQAGEIHSICGENGA
                     GKSTLMK"
     gene            783..1220
                     /locus_tag="VFC06_03405"
                     /pseudo
     CDS             783..1220
                     /locus_tag="VFC06_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037930.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(1310..1453)
                     /locus_tag="VFC06_03410"
     CDS             complement(1310..1453)
                     /locus_tag="VFC06_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80979.1"
                     /translation="MRRTQAAWVLTGELPTWETSPSTATSAPPAPEVRAAYAARRDLW
                     AQR"
     gene            1506..>2257
                     /locus_tag="VFC06_03415"
     CDS             1506..>2257
                     /locus_tag="VFC06_03415"
                     /inference="COORDINATES: protein motif:HMM:NF020897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZL80980.1"
                     /translation="MSPRDPQQVERRHVPPTVARQSSLRDQNLSVVTRTLFASGGATS
                     RAGLAERTGLTRATVSRLCQELLDAGILAEQRPAENVRPGRPGTPLAPASRTLAGVGI
                     EVNIDYMAGRALDLAGNVLAEFKKPGTYADSDPAAVLPELGDLAVGLVEQVRATGARI
                     VGTTLALPGLVESTTNTLLLAPNLNWRMVEPVPLLGSRWSPRRRDPLARAPRPGLDVP
                     RHRDHRRRTRLLPRINRRGAAGPRPRGLRSCGV"
BASE COUNT          368 a          821 c          717 g          351 t
ORIGIN      
        1 ccttcatgag cgtggacttg cccgctccgt tctccccgca gattgagtgg atctccccag
       61 cctgaaccga catcgtcacg tcgtcgagcg ctcggacacc ggggaattcc ttgatgatgt
      121 gtcgcatctc gaggatgact ctggtgtcac ccatgctcgg cctcccgaac gtcgtcgtga
      181 tcagaggcta gagcgcgcgg caacaacgac aacacgctct acctacctgc aggctcgcaa
      241 gcctgtgccc aaatgtagcg gagcgagggc gatgttggcg cctccaccag catctcgaac
      301 gtgtaacggg agattccccg cgatggcacg cggaccacag gggacctccg tcccggctga
      361 ccgaagtccg gcggtcgcgc gcggcgagta tggcgctcag catctcggac gccgtgaacc
      421 ggatcgacat tgccgggcac tgcgcgccct tcacgcgttt gggctcccgt cccgcggtct
      481 agctccccgg gccctcatcc tccgatacga aatcgacacc tccggggacg tgcgcagcgg
      541 cctttcgtcc catacgtttc ttcatgaaac aaaccggacg acgaagccac acccacggca
      601 ttcgtccaac tcggcacgtt gatccgggcg gcgatgcggg cgacatcctc acgggcatcg
      661 cgatgccgac ccgcccactg gagcgctgga acaacgacgg cgccaacctc gagactctgc
      721 tgggcgatgt aggctaccag acctcgctgc agttcgccga caacaaggtg gaccagcaga
      781 tggaactcgc cctctccccc tacctctcga tcgccgagaa catcttcctc ggcaacgagc
      841 aggccaaggg cggggtcatc gactggaaca ggaccaacgc cgaagccgcc aagctgctcg
      901 caatggtcgg cctcgactcc gacccagtga cccgcgtcgt tgagatcggc gtcgggcgcc
      961 aacagcttgt cgagatcgcc aaggcgctgt ccaaggacgt gaagctcatc atcctcgacg
     1021 agcccaccgc ggcgctcaac gacgaggact ccgccaacct gctcgccctc atggtcggcc
     1081 ggtcgctcga gaaccgttgc ccggaccacg agtccgcgat cggcgccgag gtgctgcgcg
     1141 tcgaggactg gacagtccac cacccgatcc agatggagcg caaggtggtc gacaacgcct
     1201 ccctgagcgt ccgctccggg aggtcgtcgg gctcgcgcga gcgtggcccc gacgctcgtg
     1261 acgacggcgg agtggtcacc cgccgtcagc gtgacggccg tcccgttggt caccgctgcg
     1321 cccacaggtc acggcgcgcg gcgtaggcgg cccgcacctc gggggccggg ggcgcggacg
     1381 tggcggtgga cgggctggtc tcccaggtcg ggagttcgcc ggtcagcacc cacgcggcct
     1441 gggttctcct catcgagatt tgttttatca cgaaacttac gcccgtttcg gggtatcgtc
     1501 aagacatgag tcctcgagac ccgcagcagg tggagcgccg tcacgttcca ccgaccgtag
     1561 cgcgccaatc gtccctgcgg gaccagaatc tgtccgtcgt cacccggaca ctgttcgcct
     1621 ccgggggcgc cacgtcccgg gcggggctcg ccgaacgcac cggcctgacg cgcgccacag
     1681 tgtcccggct gtgccaggag ttgctcgacg cgggcatcct cgccgagcag cgcccggcgg
     1741 agaacgtgcg tcccggccgg cccgggacgc cgctggcccc cgcatcgcgg accctggccg
     1801 gcgtcggcat cgaggtcaac atcgactaca tggcgggccg cgcgctggac ctggccggga
     1861 acgtgctcgc ggagttcaag aagccgggga catacgccga ttcggatccg gcggccgtgc
     1921 tgcccgaact cggcgacctc gccgtcggtc tggtcgaaca ggtccgggcc accggggcgc
     1981 ggatcgtcgg cacgacactg gccctccccg ggctcgtcga gtcgacgacg aacaccctgc
     2041 tgctcgcccc caacctgaac tggcggatgg tggagcccgt cccgttgctg ggttcgcgat
     2101 ggtcacctcg acgccgcgac cctctcgccc gggcgcctcg ccccggactc gacgttcctc
     2161 gacatcgaga tcaccggcgc cgcacccgac tcctcccccg catcaaccgg cggggcgctg
     2221 cgggccctcg accccgtggt ctccgatcct gcggagt
//
LOCUS       DASRKH010000207         2383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_43911, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000207 DASRKH010000000
VERSION     DASRKH010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43911"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="VFC06_03420"
     CDS             <1..747
                     /locus_tag="VFC06_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZL80981.1"
                     /translation="AFTYPGTDRPVLEGVDLVLPAGSTVAVVGENGAGKTTLVKLLCR
                     FYDATAGTVRVDGVDLRDIDTDEWRTRIAAGFQDFSRFELLARENVGVGNLPDIESQT
                     AVLAGLRRARAEGVLDRLDDGLETQLGTSHTDGAQLSGGQWQKLALGRAMMREAPLLL
                     VLDEPTSALDAQAEHELFEQYAVNARRVGGLTGAITLLVSHRFSTVRMADLILVVADG
                     RVTESGSHDELVALGGLYAELFDLQAAAYR"
     gene            992..>2383
                     /locus_tag="VFC06_03425"
     CDS             992..>2383
                     /locus_tag="VFC06_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HZL80982.1"
                     /translation="MTTISSGQGATRHREASPIDAIADAYVATSAALDPLAATAMGIA
                     GHDHEMTDLSPAGHAARAEAARSTLVALDAHAPSDATDEVTLAAMQERLGLELELDAA
                     GETLADLNNIASPVQGLRDVFDLMPTGSIEGWENIAARLGALPGAVDGYVESLRESAS
                     RGRVAAIRQVRDAIAQAGDLADPKTSFFTSFVHGPTVDLVLDDSSSCSLVRAELERGA
                     TAARGAYATLTAFLRDELAPQAPAADAAGRDRYALWSRYFLGATVDLEETYQWGLEEL
                     ARVIAEQERVAEQIAGPGATVEQAVAVLDADPALTLHGTAELRAWMQETSDAAIAALN
                     GTHFDIPVPVQTLECRIAPTQNGGIYYTGPSDDFSRPGRMWWSVPPSVTTFNTWRRLA
                     SWTSGHGEGWALYAERLMADLGFLDNPGDRLGMLDGQRMRAARVVLDIGVHLGLPAPE
                     RWGGGTWDADKAWE"
BASE COUNT          334 a          879 c          846 g          324 t
ORIGIN      
        1 gcgttcacgt acccgggaac ggaccgaccc gtcctcgagg gcgtcgacct ggtgctgccg
       61 gcgggctcca ccgtcgccgt cgtcggcgag aacggcgcgg gcaagacgac gctcgtcaag
      121 ctgctgtgcc gcttctacga cgcgaccgcg gggacagtgc gcgtcgacgg agtcgacctg
      181 cgggacatcg acaccgacga gtggcgcacc cgcatcgccg ccgggttcca ggacttctcg
      241 cgcttcgagc tgctcgcccg agagaatgtg ggcgtcggga acctcccgga catcgagtcg
      301 cagaccgccg tgctcgcggg gctgcggcgc gcccgcgcgg agggggtgct cgaccggctc
      361 gacgacgggc tcgagacgca gctcgggacg tcccacaccg acggtgccca gctctccggc
      421 ggccagtggc agaagctcgc cctcggccgc gccatgatgc gcgaggcgcc gctcctgctt
      481 gtcctcgacg aaccgacctc cgctctcgat gcgcaggccg agcacgagct gttcgagcag
      541 tacgcggtca acgcgcgccg ggtcggcggg ctcacgggcg cgatcaccct gctcgtgtcg
      601 caccggttct cgaccgtgcg gatggccgac ctcatcctcg tcgtcgcgga cggtcgggtc
      661 acggagtccg gttcgcacga cgagctcgtc gcgctcgggg ggctgtacgc cgagctgttc
      721 gacctgcaag cggcggccta tcggtgagcg ggacctcgcc gcaccggtcg gcttcaggtt
      781 gagtctgcgg gcggccactg actcgggtcg ccatcttcga gcggcgcacg tcgcaggcgg
      841 agctcctcat cgccgcggtg gtcttcggca tcgcgcagga gccgatcacc aacctgctcg
      901 aggcacgggc gcgcaaggtg caggacgagg ccaccgacac cgacaagccg gagtgagggt
      961 gggtcgctca ccctggcgat aaggtcaagc aatgaccacg atctcctccg gacagggtgc
     1021 gacacggcac cgcgaagcct ctcccatcga cgcgatcgcc gacgcctacg tcgccacgtc
     1081 tgcggccctc gacccgctcg cggcgaccgc catggggatc gcgggccacg accacgagat
     1141 gaccgacctg tccccggcag gccacgcggc ccgcgccgag gccgcgcgca gcactctcgt
     1201 cgctctcgac gcgcacgcgc cgagcgacgc gaccgacgag gtcacgctcg ccgcgatgca
     1261 ggagcgcctc gggctcgagc tcgagctcga tgctgccggc gagacgctcg cggacctcaa
     1321 caacatcgcc tcacccgtcc agggcctgcg cgacgtgttc gacctcatgc cgacggggtc
     1381 gatcgagggc tgggagaaca tcgccgcgcg cctgggcgcc ctcccgggcg ccgtggacgg
     1441 ctacgtcgag tcgctgcgcg agtccgcatc gcgcgggcgc gtcgccgcga tccgccaggt
     1501 gcgggacgcg atcgcgcagg cgggcgacct cgccgacccc aagacgtcgt tcttcacgtc
     1561 gttcgtgcac ggtcccacgg tggacctcgt gctcgatgac tcgtcctcgt gctcgctcgt
     1621 gcgcgccgag ctcgagcgcg gcgcgaccgc cgcccgcggg gcgtacgcga cgctcacggc
     1681 tttcctgcgc gacgagctgg ccccgcaggc ccccgcggcc gacgccgcag gccgcgaccg
     1741 ctacgcgctg tggtcgcggt acttcctcgg tgcgacggtc gacctcgagg agacctacca
     1801 gtgggggctc gaggagctcg cacgcgtgat cgccgagcag gagcgcgtcg ctgagcagat
     1861 cgcggggccc ggggcgaccg tcgagcaggc cgtcgcggtg ctcgacgccg acccggcgct
     1921 cacgctgcac ggcacggccg agctgcgggc gtggatgcag gagacgtcgg acgcagcgat
     1981 cgccgcactc aacggcacgc acttcgacat ccccgtcccc gtgcagaccc tcgagtgccg
     2041 catcgctccg acgcagaacg gtggcatcta ctacaccggc ccgagcgacg acttcagccg
     2101 ccccggtcgc atgtggtggt ccgtgccgcc gtcggtcacg acgttcaaca cgtggcgccg
     2161 gctcgcgagc tggacctcag ggcacggcga gggctgggcg ctctatgccg agcgcctcat
     2221 ggccgacctc ggcttcctcg acaaccccgg cgaccggctc gggatgctcg acgggcagcg
     2281 catgcgtgcc gctcgcgtcg tcctcgacat cggcgtgcac ctcggtctgc cggcgcccga
     2341 gcgctggggc ggcgggacgt gggacgcgga caaggcgtgg gag
//
LOCUS       DASRKH010000208         2298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_55127, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000208 DASRKH010000000
VERSION     DASRKH010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55127"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VFC06_03430"
     CDS             <1..266
                     /locus_tag="VFC06_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HZL80983.1"
                     /translation="KVTKKHDVATTPHQRAVAHEAVRPRPKITMNAEFKRIKPAALSR
                     HILALTGELEVLAQVKKSPRAMSPVNHTWNDSDWRRKPREATS"
     gene            complement(869..1252)
                     /gene="rplL"
                     /locus_tag="VFC06_03435"
     CDS             complement(869..1252)
                     /gene="rplL"
                     /locus_tag="VFC06_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HZL80984.1"
                     /translation="MAKLTTEQLIDAFKELTLIELSEFVKAFEETFDVTAAAPVAAAA
                     AAPAAAAAEEVEEQVDFDVVLATVGDKKIQVIKEVRALTSLGLGEAKALVDEAPKTIL
                     EGVKKEDAEKAKAQLESAGATVELK"
     gene            complement(1307..1828)
                     /gene="rplJ"
                     /locus_tag="VFC06_03440"
     CDS             complement(1307..1828)
                     /gene="rplJ"
                     /locus_tag="VFC06_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HZL80985.1"
                     /translation="MATPDKVATVKALADQFRSAQAVYVTEYRGLSVAQLKQLRKGLS
                     GTATYAVVKNTLAARAAKDAGLEGLETLFVGPTAIAFVTGDPVEAAKGLRDFSKESDK
                     LVLKGGILEGNLLSAADLSKLASLESREVLLAMAAGAFKASLFGAAYLLQAPLSQAVR
                     TVDALREKQDTAA"
BASE COUNT          403 a          771 c          724 g          400 t
ORIGIN      
        1 ccaaggtcac caagaaacac gatgtcgcga ccaccccgca ccagcgtgca gtcgcccacg
       61 aggctgtccg gcctcgaccg aagatcacca tgaacgcaga attcaaacgg atcaagcctg
      121 cagccctgtc gcgccacata ctggccctga ccggcgagct cgaagtactg gcccaagtca
      181 agaaatctcc ccgcgccatg tccccggtca accacacctg gaacgactcc gactggcgga
      241 ggaaaccacg tgaggcaacg tcataacctt cccggaggta ttgacatgag gcaacagggc
      301 gcggactata gacgatgccc ggcctgctgg tcgaggcctg catcggcgcg tctggggacg
      361 ggcgactttc caccgtacag cgggcactcg ctcgcagagg ctcggcccgc gccctccgac
      421 tcgcgcgccc tccgactcgc gcgccctccg actcgcgcgc cctccgactc gcgcgccctc
      481 cgactcgcgc gccctccgac tcgcgcgccc tctgactccc gcgcccgttc gaaccggctg
      541 gatcatcgac gcgccagttt cggggtcgtc caccgctcca acgccggcgt gtagtgctcg
      601 ccgccggttc gtagcgtctg ccgccccacc atgacatgcc ggatatgaac agttctgacc
      661 gcgtaggcgt ccgacccgcc ggcacggtgc gctcagactc tgcgcgttgc ctacttcgcg
      721 gtcaggagtg tccagccggc gcaggcaggc acagcgacgg cgcaggcagg cacagcgacg
      781 ggcccctccc gccaccggcc tcagcaacgg cacaggccgg gccacccgaa ggtgacccgg
      841 cctgtgcgcg agttgcgtga agtgcgaact acttgagttc gaccgtggcg ccggcggact
      901 cgagctgagc cttggccttc tcggcgtctt ccttcttgac accctcgagg atcgtcttgg
      961 gagcctcgtc gacgagagcc ttggcctcgc ccaggccgag ggacgtgagg gcacgcacct
     1021 ccttgatgac ctggatcttc ttgtcgccaa cggtggccag aacgacgtcg aagtcgacct
     1081 gctcttcgac ctcttcggca gcggcggcgg ccggagctgc agcagcggcg gcaaccggag
     1141 cggctgcggt gacgtcgaag gtctcttcga atgccttcac gaactcggag agctcgatga
     1201 gcgtgagctc cttgaatgcg tcaatgagct gctcggtggt gagcttcgcc ataatgatgt
     1261 ccttttcttt gtacctgcgg gaagcagtgt gaggtggggt gcagccttag gctgcggtgt
     1321 cctgcttctc gcgcagggcg tcgacggttc gcaccgcctg agacaggggt gcttggagga
     1381 ggtacgccgc gccaaagagc gacgccttga aagcaccggc tgccatggca agtagcacct
     1441 ctcgagactc gaggctcgcg agcttggaga ggtccgcggc ggagagcaga ttgccctcca
     1501 ggatgccgcc cttaaggacg agcttgtcgc tctccttaga gaaatcacga agacccttcg
     1561 cagcctccac cgggtcaccg gtgacgaatg cgatggccgt gggacccacg aagagggttt
     1621 cgaggccctc taggccggca tccttggccg cacgcgccgc aagcgtgttc ttcacgaccg
     1681 cataggtcgc tgtaccgctg aggcccttgc gcagctgctt gagctgagca acggacaagc
     1741 cgcggtactc ggtgacgtag acggcctgtg cactacggaa ctggtccgca agcgccttga
     1801 ctgtcgccac cttgtctggc gtcgccatag ttgtccttcc tgtggttgac caccgggcct
     1861 gctccacaca atgaaaaagc cccgcgcagg cggggctgca gcgccacatc cacacacacg
     1921 aagggcggcc acatgggccg cccaaagtct gcgcgcgagg cgccgtcaac ttctcctgcg
     1981 ctggtctccg tttttgcgga acttcggccg ttcccggttt cccgggcccg acgaccggcg
     2041 atcttgggcg tgggccatca tacgggacga agtccacgcc gtgcaaacct gcgacgtgcc
     2101 tcagtttctt tgagcgcgag ggatgggttg gtgcctcagc atctttgagc gcgtagcccc
     2161 tcgtttgggg gcaggcttgc ctgatggtag aaggcaagat tccgatggcc gcgaggcgac
     2221 aagtcacgaa caagctccgg accgtttacg cgcgagcgtc gaagcgggac aaggccagga
     2281 tcctcgatga ggtgatgt
//
LOCUS       DASRKH010000209         2116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_56177, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000209 DASRKH010000000
VERSION     DASRKH010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56177"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VFC06_03445"
     CDS             <1..352
                     /locus_tag="VFC06_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80986.1"
                     /translation="FVISTYDKNSLLSLQRNDSYDGILGEAKSSSVILKYFADASNLK
                     PRELAPELNDRGVRRKGYRWDRFKAAWSRFYFEDRVEPITPAELKEAQDHHVHEVEHM
                     ASSAKTEEPTVTEV"
     gene            complement(401..1915)
                     /locus_tag="VFC06_03450"
     CDS             complement(401..1915)
                     /locus_tag="VFC06_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HZL80987.1"
                     /translation="MSPFIRRVKTSSGATAVQIVEKRRGVRTILRHIGSAHTDAELAL
                     LMVVAQQSLHEGQDTLDLGLEAAPVPRSEARVLSSSSELLWAVLTDAYQALGFDGLDD
                     DAFRALVLARIVEPTSKADTVRVLNEIGAPAPHVNTLHTALKRASERDYRDQIARSCM
                     THSLKTTGPAMFVLYDVTTLHFENDDEDALRRVGMSKEHRVDPQVQVGLLVDPAGLPL
                     EMHLFEGNKAETTTLIPVLRAFQDRHHISDVVVVADAGMLSAGNLNALEDAGLAFVVG
                     SRLTKAPYDLEEHFTAHGNHFADGQILESSRVMGTGPAARQRRVVYQWSLKRNKRDDR
                     NINLMIAKAEKIAAGTIPLRKARFLKVSGARKELDQATIDRARQLAGIKGYVTNLPQS
                     VMPGSGVIGAYHDLWRVEQSFRMTKSDLKARPVFHHRRDAIEAHLTIVFAALAITRYL
                     TSATGVSIKKLVRTLQPLRSVRIGIGPHELTAEPQINAAARTILNRLPPITVGH"
BASE COUNT          377 a          658 c          697 g          384 t
ORIGIN      
        1 gttcgtcatc tccacctatg acaaaaactc gctgctcagc ctgcagcgca acgattccta
       61 cgacggtatc ctgggcgagg cgaaaagcag ctcggtgatc ctcaagtact ttgccgacgc
      121 cagcaacctc aagccgcgcg aactggcccc cgagctcaac gaccgtggcg tgcgccgcaa
      181 gggctaccgc tgggatcgct tcaaggcggc ctggtcgcgc ttctacttcg aggaccgcgt
      241 ggagccgatc acgcctgccg aactcaagga ggcgcaggac catcacgtac atgaggtgga
      301 gcacatggct tcttcggcca agaccgagga gcccacggtc accgaagtct gatctccacg
      361 atgtcgaggc ggcgcgcacc cagtcccgac tctcacaggt ctagtgcccc acggtgatgg
      421 ggggtaggcg gttgaggatg gtgcgggcgg cggcgttgat ctggggttcg gcggtgagtt
      481 cgtgggggcc gatgccgatg cggactgatc gcaggggttg cagggttcga acgagtttct
      541 tgatcgacac cccggtcgcg gaggtgaggt atcgcgtgat ggcgagggct gcgaacacga
      601 tggtcaggtg cgcttcgatt gcgtcgcgcc ggtggtggaa tacggggcgg gctttcaggt
      661 cggacttcgt catgcggaag gattgctcga cgcgccatag gtcgtggtag gcgccgatga
      721 cgccgctgcc aggcatgacg gactggggca ggttcgtaac gtagcccttg atacccgcca
      781 gctgcctcgc gcggtcgatc gtggcctggt cgagttcctt acgcgccccc gacactttca
      841 agaagcgtgc tttgcgcaaa gggatggtgc ccgcagcgat cttctcggcc ttggcgatca
      901 tgaggttgat gttgcggtcg tcgcgcttgt tgcgtttcag cgaccactgg tacacgaccc
      961 tgcgctggcg agccgccggt ccggtgccca tcacacgtga ggactcgagg atctgcccgt
     1021 cggcgaagtg gttgccgtgg gcggtgaagt gctcctcgag gtcgtagggg gccttcgtca
     1081 gccgggaccc gacgacgaac gcgagcccgg cgtcctcgag ggcgttcaaa ttgccggcgg
     1141 acagcatgcc cgcgtcggcg accactacca cgtcactgat gtggtgacgg tcctggaagg
     1201 ctcgcaggac ggggatgagg gtggtcgttt cggccttgtt gccctcgaac aggtgcattt
     1261 ctaacggcaa ccctgccggg tcgacgagga gaccgacttg gacttgcggg tccacgcggt
     1321 gttccttgct catccccaca cggcgcaggg cgtcctcgtc atcattctca aaatgcaacg
     1381 tcgtcacgtc gtacaacacg aacatcgccg gaccggtggt cttgagcgag tgcgtcatgc
     1441 aggagcgggc gatctggtcg cggtagtcac gttcactggc gcgcttgagc gccgtgtgga
     1501 gggtgttcac gtgcggggcg ggggcaccga tctcgttcag cacccgcacg gtatcggctt
     1561 tggaagtcgg ctcgacaatc cgggccagca cgagggcacg gaacgcgtcg tcgtcgagcc
     1621 cgtcaaaacc caatgcttgg taggcgtctg tgaggaccgc ccacagcagc tcgctactac
     1681 tggacagcac ccgcgcttca ctgcgcggca caggggcagc ctcaagtccg agatcgaggg
     1741 tgtcttgccc ttcatgcaag gactgctgcg cgacgaccat cagcagcgcc agctcagcat
     1801 cggtatgggc ggacccgatg tgcctgagga tcgtgcgcac cccgcgacgc ttctcgacga
     1861 tctgaaccgc cgtcgccccc gaggacgtct tcacacggcg aatgaacggg ctcaccggcc
     1921 aagactaccg ccccactagt gccccaaatc agcccacccc aagccatcta gcaggacaaa
     1981 cactcaagaa cccgaaacac ctgtgagagt caggcccgag tgtccgtagc cctccggcct
     2041 actccaccgc acccgccctc ccgactctca caggtctagt gccccacggt gatggggggt
     2101 aggcggttga ggatgg
//
LOCUS       DASRKH010000210         2373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_38861, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000210 DASRKH010000000
VERSION     DASRKH010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38861"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(193..1773)
                     /locus_tag="VFC06_03455"
     CDS             complement(193..1773)
                     /locus_tag="VFC06_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015884054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HZL80988.1"
                     /translation="MRLRTVLAAAATTCLLVALAGPAVAGASGGQHPTPGTGSLTRAD
                     RADLQRWATDTWASFAAMTDESTGLPADNIDADLSAASRSGYTSPTNIGAYLWSTVVA
                     EKLGIIGHRETRTRLTRTLTTLSTMERHAPSGMFYNWYDEATGNKLTVFPDGEHVDPF
                     LSSVDNGWFAAALMVVRNAEPAVRKQADALLAGMDFGAFYDANQFGGTRPGMVRGGFW
                     VDPPSQNACPGTLPDGTPVQFTCNHYDILDSEPRIVTYIGISRGQIPPEAYFATYRTF
                     PASCDWSWPEQRPVGETRSYLGLDVFEGAFTYRGMHIVPTWGGTMFEELMPDLFVPES
                     SWGTKSWAVNHPLAVRAQIEHGLDDAGYGYWGFSPASNPFGGYSVYGVDEIGMDPGGY
                     PSDREGTNVDKGYDGCRDATNPTPTFGDGVVTPHASFLALPYAPRATVDNLRGIEDTL
                     GAYGAGGFYDSVATRSGTAAKRYLSLDQGIIMGSLGNVLGENLLHDAFSKGAVERTVK
                     PLLALEQFGSGLVAETTP"
     gene            complement(1925..>2373)
                     /locus_tag="VFC06_03460"
     CDS             complement(1925..>2373)
                     /locus_tag="VFC06_03460"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HZL80989.1"
                     /translation="HIAIEPDVEDLVVHHDLAPWNLVLAEDRLVLIDWDGCGPGSRLW
                     DLAYAAHGFVPLAPPDAGGPGTDAAASALRELADGYGLDDDGRVRLAGLLARRTWSMY
                     DLLDRGHRTGTQPWARLWDEGHGTSWRNDAEWIEAHGAALRTALTR"
BASE COUNT          328 a          829 c          875 g          341 t
ORIGIN      
        1 gacgatcgcg accggtgtcg aggccgagcg gcaggacgag gcccgcgcgt actaccgggc
       61 gcagcgtgcg tcggaggacg cgccgctcgc ggagaagcag ctaggcaagc gctgacctgc
      121 acgcgacgga cgcggccctc accccgggga ctcaggggat gagggccgcg cccgtcggcg
      181 gcgcggctcg cgtcagggcg tcgtctcggc gacgagtccc gacccgaact gctccagcgc
      241 gaggagcggc ttcaccgtcc gctcgaccgc acccttgctg aaggcgtcgt gcagcaggtt
      301 ctcgccgagc acgttgccga gcgaacccat gatgatgccc tggtcgagcg acaggtagcg
      361 cttggccgcg gtgccgctgc gggtcgccac ggagtcgtag aagccgcccg caccgtaggc
      421 gccgagggtg tcctcgatgc cgcgcaggtt gtcgaccgtg gcgcgcgggg cgtacggcag
      481 cgcgaggaac gacgcatgcg gtgtgacgac gccgtcaccg aacgtcgggg tcgggttggt
      541 cgcgtcgcgg cacccgtcgt agcccttgtc gacgttcgtg ccctcgcggt cggacgggta
      601 cccgcccggg tccatcccga tctcgtcgac gccgtagacg ctgtagccgc cgaacgggtt
      661 gctcgcgggc gagaagcccc agtagccgta gccggcgtcg tccaggccgt gctcgatctg
      721 cgcgcggacc gcgagcgggt ggttgaccgc ccagctcttg gtgccccagc tcgactccgg
      781 cacgaacagg tccggcatga gctcctcgaa catggtcccg ccccacgtcg ggacgatgtg
      841 catgccgcgg tacgtgaacg cgccctcgaa cacgtcgagg cccaagtagc tgcgggtctc
      901 gccgaccggg cgctgctcgg gccacgacca gtcacagctc gccgggaacg tgcggtacgt
      961 cgcgaagtac gcctcgggcg ggatctgccc gcgcgagatg ccgatgtacg tcacgatccg
     1021 gggctcggag tcgaggatgt cgtagtggtt gcaggtgaac tgcaccgggg tgccgtcggg
     1081 cagggtcccc gggcacgcgt tctgcgacgg cgggtcgacc cagaagccgc cgcgcaccat
     1141 gccgggccgg gtcccaccga actggttcgc gtcgtagaac gcgccgaagt ccatcccggc
     1201 gagcagcgcg tcggcctgct tgcggactgc gggctcggcg ttgcgcacga ccatgagggc
     1261 cgccgcgaac cagccgttgt cgacgctcga gaggaacggg tcgacgtgct cgccgtccgg
     1321 gaacaccgtg agcttgttgc ccgtggcctc gtcgtaccag ttgtagaaca tcccgctcgg
     1381 cgcgtgccgc tccatggtcg agagggtcgt cagggtccgg gtcagccgcg tgcgcgtctc
     1441 gcggtgaccg atgatgccga gcttctccgc gacgacggtg ctccacaggt acgcgccgat
     1501 gttcgtcggc gacgtgtacc cggaccgcga cgccgccgac aggtcggcgt cgatgttgtc
     1561 ggccgggagg cctgtgctct cgtcggtcat cgctgcgaac gacgcccagg tgtcggttgc
     1621 ccagcgctgg agatccgcgc ggtccgcacg ggtcagcgag ccggtgcccg gggtcggatg
     1681 ctgcccgccg gacgcgccgg ccacggccgg cccggcgagg gcgaccagga ggcaggtcgt
     1741 ggcggccgca gccaggactg tacggagtct catcagtgag tccttgtcgt cagtgacggg
     1801 tgcacgggtg cacgggtgca cgggtgcacc gggcgctgaa cgcgccgcgc gacgcctcgt
     1861 tgcgtgacga ccatcacacc cgttcgggtg acgtgcaacc gttcgggtga ctcgccccgc
     1921 gcagtcagcg ggtgagagcc gtgcgcagcg cggcgccgtg cgcctcgatc cactcggcgt
     1981 cgttccgcca cgacgtgcca tgaccctcgt cccacagccg cgcccacggc tgtgtcccgg
     2041 tgcgatgccc ccggtcgagc aggtcgtaca tgctccaggt gcggcgcgcg agcagaccgg
     2101 cgaggcgcac gcgtccgtcg tcgtccagcc cgtagccgtc ggcgagctca cggagggccg
     2161 acgcggccgc gtccgtcccc ggcccgccgg cgtccggcgg agcgagcggc acgaatccgt
     2221 gcgccgcgta ggcgaggtcc cacaggcggc tgcccggccc gcacccgtcc cagtcgatga
     2281 gcaccagccg gtcctcggcg agcacgaggt tccacggcgc gaggtcgtgg tgcacgacga
     2341 gatcctcgac atcgggctcg atcgcgatgt gcc
//
LOCUS       DASRKH010000211         3443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_53492, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000211 DASRKH010000000
VERSION     DASRKH010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53492"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(252..917)
                     /locus_tag="VFC06_03465"
     CDS             complement(252..917)
                     /locus_tag="VFC06_03465"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HZL80990.1"
                     /translation="MTNAWREQVAPDWAAALEPVAPLLADIGGFLRAELESGRTYLPA
                     ASAILNAFTRPLADVRVLIVGQDPYPTPGDPMGLSFSVPRGRPMPRSLANIARELKAD
                     TGEDLASGDLTAWADQGVMLLNRCLTVRPGESGSHRGHGWEAFTEHAIRTLAARGGPL
                     VAILWGKDAQGLAPMLGDVPRIESAHPSPLSASRGFFGSRPFSQANALLKTQGAPPVV
                     WGT"
     gene            1005..1301
                     /locus_tag="VFC06_03470"
     CDS             1005..1301
                     /locus_tag="VFC06_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005274838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3263 domain-containing protein"
                     /protein_id="HZL80991.1"
                     /translation="MSAVSVNAPSPGVASLSERDERILAFERQWWKFAGSKEDAVREE
                     FGMSATEYYQVLNALIDSEPALAHDPMLVKRLRRMRASRQRGRAGRAIGASHQR"
     gene            1311..1904
                     /locus_tag="VFC06_03475"
     CDS             1311..1904
                     /locus_tag="VFC06_03475"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HZL80992.1"
                     /translation="MPDFPVDEFDEAAEERGPMGVHRKRKRILWAIAAPLLIFIGAGA
                     LAYGVVVYLWRAQGNEGIPPIDSGPRATIVTTLAPTTDVTASPTVSATPSTTPSPTPT
                     VEPVQFGASVSVLNGARIAGLAGANQTKLEAAGFTAASAGNITGTKPPENTVRYADAG
                     LESTAAKVAEVLGITNIERGVVSDGDITVLLVTDPAA"
     gene            2128..>3443
                     /gene="groL"
                     /locus_tag="VFC06_03480"
     CDS             2128..>3443
                     /gene="groL"
                     /locus_tag="VFC06_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012243936.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HZL80993.1"
                     /translation="MAKIIAFDEEARRGMERGMNALADAVKVTLGPKGRNVVLEKKWG
                     APTITNDGVSIAKEIELDEPFEKIGAELVKEVAKKTDDVAGDGTTTATVLAQALVTQG
                     LRNVAAGANPIALKRGIEKAVEAVTRELMAAAKDVETKEEIAATAGISAGDPAIGDLI
                     AEAMDKVGKEGVITVEESSALGLDLELTEGMRFDKGFLSAYFVTDSERQEAVLEDTYV
                     LLVENKISNIKDLVPVLEKVMQTGKALAIIAEDVESEALATLVVNKLKGTFKAVAVKA
                     PGFGDRRKAMLQDMAILTGGTVISESVGLSLENTTLDLLGQARKVVVTKDETTIVEGA
                     GSEEQLNGRVKQIRAEIENSDSDYDREKLQERLAKLAGGVAIIKAGAATEVELKERKH
                     RIEDAVRNAKAAVEEGIVAGGGVALIQAGIRAFATLELEGDEATGAR"
BASE COUNT          676 a         1159 c         1092 g          516 t
ORIGIN      
        1 attctcgacg aactgagcag catcgaggag accgtgctgc tctaccacga cggcgccaag
       61 ggccgcccgc gcgcccaacg cctcctcacc gatcattcca agaccgcggc caaactcgcc
      121 aaactgttca acctcgacac acacgcccca acccgataac ccgcagaaca ggatcaccta
      181 ggtaatacac cccaaacacc ccctgaccag cacaaacacc accaaaaccg gtcacacctc
      241 ggaaactcgc gctaggtgcc ccagacgacc gggggagccc cttgggtctt gagcaacgcg
      301 ttggcttgag aaaatggccg cgacccaaag aatccccgcg acgcgctgag cgggctgggg
      361 tgggccgact cgatgcgagg gacgtccccg agcatcggcg ccaacccttg ggcgtccttg
      421 ccccacaaga tcgcgacgag cggcccgccg cgagcggcca aggtgcgaat ggcgtgctcc
      481 gtgaaggcct cccacccgtg acctcggtgg gaccctgact ctcccggccg caccgtcaga
      541 cacctgttga gcagcatcac gccctgatcg gcccacgctg tgaggtcgcc gctggcgagg
      601 tcttctcccg tgtcggcctt gagttcgcgc gcgatattgg cgagggaccg cggcatcggc
      661 cgcccgcgcg ggaccgagaa ggacaggccc atcgggtcgc cgggcgtcgg gtacgggtcc
      721 tggccgacaa tgagcacccg gacgtctgcc aggggcctcg tgaacgcgtt gaggatggcg
      781 gacgccgctg gcaggtaggt tcgacccgac tccaattcgg cccgcaggaa cccgccgatg
      841 tccgccagga gcggcgcgac cggctcgagc gcggccgccc aatcgggtgc cacctgctcc
      901 cgccacgcgt tggtcatggc ctcagcgtat ccacgcgaga agcctgcgcg gcggagcggt
      961 cgcaatcgaa tgacaaccat gtgattcgcg ggtactctgg ggacatgtct gcagtctcgg
     1021 ttaacgctcc ttctcctggc gttgcgtctc tcagcgagcg cgacgagcgc atcttggctt
     1081 tcgagcgcca atggtggaag ttcgccggct ccaaggaaga cgcggtgcga gaggagttcg
     1141 gcatgtccgc cacggagtac taccaggtgc tcaacgcgct gatcgactct gaaccggcgc
     1201 tcgcccacga ccccatgctc gtcaagcggc tgcgccgcat gcgggcatcc cgccaacgcg
     1261 gccgcgcggg tcgggccatc ggtgcctcgc accagcggta acgtgtgcac gtgcccgact
     1321 tccctgtaga cgaattcgac gaggccgctg aagagcgcgg ccccatgggc gtgcaccgca
     1381 agcgcaagcg catcttgtgg gcaatcgcgg ctcccctcct catcttcatc ggcgcgggcg
     1441 cgctcgcgta cggcgtggtc gtgtacctct ggcgggcaca aggcaacgaa ggcatcccgc
     1501 ccatcgactc cggtccccgc gcgacgattg tcacgacgct ggcaccaacg accgatgtca
     1561 ccgcatcgcc caccgtgtcg gcaacgccgt caaccacccc gagtcccacg cccacggtgg
     1621 aacccgttca gttcggcgcg tcggtgtcgg tactcaacgg cgcgcgtatc gctgggctcg
     1681 cgggagcgaa tcagacgaag cttgaagcgg cgggcttcac agccgcgtcg gcaggcaaca
     1741 tcaccgggac caagccgccc gaaaacaccg tcaggtatgc ggatgcgggc ctggagagca
     1801 ctgcagccaa ggtggccgag gtgctcggga tcaccaacat cgagcgtggc gtggtgtccg
     1861 atggcgacat caccgtgttg ctcgtgactg acccggccgc ctagcaatcc cctccaggca
     1921 ttcttcgccg cgcgcgaaag tgggctgcgc catttgcact ctcgggggga gagtgccaaa
     1981 atctcattag cactcgcact acgagagtgc taagacttcg gcgccaaaga tgaggcgttc
     2041 cgacgatgtg atctgaacat cgttgtgggc gtcgtccgtc gcgggcatca gcggcgccac
     2101 aacccatcga aagaggaata cacacccatg gcaaagatca ttgccttcga tgaggaggcc
     2161 cgtcgcggta tggaacgggg catgaatgcc ctggccgatg ccgtgaaggt caccctcggt
     2221 cccaagggcc gcaacgtggt gctcgagaag aagtggggcg cccccaccat cactaacgat
     2281 ggcgtgtcca tcgccaagga gatcgagctc gacgaaccct tcgagaagat cggtgctgag
     2341 ctcgtcaagg aggtcgccaa gaagaccgac gacgtcgctg gcgacggcac caccaccgcc
     2401 accgttctcg cccaggccct ggtcacccag ggtctgcgca atgtcgccgc aggcgccaac
     2461 ccgatcgccc tcaagagggg tatcgagaag gccgtcgagg ccgtcacgcg cgaactcatg
     2521 gcagccgcca aggacgtcga aaccaaggaa gagatcgccg caaccgctgg catctcggcc
     2581 ggcgaccccg ccatcggcga cctcatcgcc gaagccatgg acaaggttgg caaggaaggc
     2641 gtcatcaccg tcgaggaatc ctcggcgctg ggactcgacc ttgaactgac cgaaggcatg
     2701 cgcttcgaca agggcttcct gtcggcctac tttgtgaccg actcggagcg ccaagaggcc
     2761 gtcctcgaag acacctacgt gctgctggtc gagaacaaga tctcgaacat caaggacctc
     2821 gtcccggtgc tcgagaaggt catgcagacc ggcaaggcgc tcgcgatcat cgccgaggac
     2881 gtcgagtccg aggctctcgc cacgctcgtg gtcaacaagc tcaagggcac gttcaaggcc
     2941 gtcgccgtca aggcgcccgg ctttggcgac cgtcgcaagg cgatgcttca ggacatggcc
     3001 atcctcacgg gtggaaccgt catctcggag tcggtgggtc tctcgctgga gaacaccacg
     3061 ctcgaccttc ttggccaggc ccgcaaggtg gttgtcacca aggacgagac gaccatcgtc
     3121 gagggcgcgg gcagcgaaga gcagctcaac ggccgcgtca agcagatccg cgccgagatc
     3181 gagaactcgg actccgacta cgaccgcgag aagttgcagg agcgtctggc caagctggcc
     3241 ggcggcgttg caatcatcaa ggccggagcc gcgaccgagg tcgagctcaa ggagcgcaag
     3301 caccgcatcg aggatgcagt gcgcaacgcc aaggccgctg tggaagaggg catcgtcgcc
     3361 ggtggtggcg tcgcactcat ccaggccggc atcagggcgt tcgctactct cgagctcgag
     3421 ggtgacgagg cgaccggtgc tcg
//
LOCUS       DASRKH010000212         2071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_32443, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000212 DASRKH010000000
VERSION     DASRKH010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32443"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..114
                     /locus_tag="VFC06_03485"
                     /pseudo
     CDS             1..114
                     /locus_tag="VFC06_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016667411.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(105..1607)
                     /gene="lepA"
                     /locus_tag="VFC06_03490"
                     /pseudo
     CDS             complement(105..1607)
                     /gene="lepA"
                     /locus_tag="VFC06_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011692084.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
     gene            1599..2071
                     /locus_tag="VFC06_03495"
                     /pseudo
     CDS             1599..2071
                     /locus_tag="VFC06_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011692084.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
BASE COUNT          391 a          676 c          651 g          353 t
ORIGIN      
        1 gacatctccc gcaagcgcaa gctgctggag aagcagaagg ccggaaagaa gcgcatgaag
       61 aacatcggct cggtcgaggt gccgcccgaa gcgttcatcg ccgcgctgag cacgtccttg
      121 cgcatcgctc gaatggtctc gcgggcgatg atgcgggagc cgatcgccgc ctggatcggc
      181 acctcgaact gctggcgctc gatcaggtcc ttgagcttgc cgaccatcga cagcccgtac
      241 ttgtacgccg cgtccttgtg gaccactgcg ctgaaggcgt cgaccggttc gccctgcagc
      301 aggatgtcca ccttgaccag gtcggaagcc ttctcgccgg cgggttcgta gtccaaggat
      361 ccatagccgc gagtgcgcga cttgagctga tcgaagaagt cgaacaccac ctcggcgagc
      421 ggcaacgtgt agtgcaactc cacacgcaac tcactgaggt aattcatcgt gcccatcacg
      481 ccgcggcgct cctggcacag ctccatgacg gtgccgatga actcgctcgg caccagaatc
      541 gacgcgttca ccatcggttc gcggacttcc atgatcttgc ccgcagggaa ctcggagggg
      601 ttggtgacga tgtggacctt gccgtcctcc atctccacct cgtacaccac gttgggcgcg
      661 gtagagatga ggtcgatatc gaactcgcgc tcgaggcggt cgcgcacaat ctcgagatgc
      721 agcaggccta gatatccgca acggaagccg aagccgagcg ccaccgaatt ctcgggctcg
      781 aagacgagag acgcatcgtt gagctgcaac ttgtcgagag cgtcgcgcag gatcgggaag
      841 tccgaaccgt caagcgggaa cagcccggaa tacaccatgg ggcgcggatc ccgataccca
      901 ccaagagact ccttggcacg gttgttcgcg agcgtgaccg tatctcccac cttcgagagc
      961 cgcacgtcct tcacaccggt gatgaggtag cccacctccc ccacgcgaag gcccttggtg
     1021 ggggtcggct cggggctgat gacgccgatc tcgagcagct cgtgggtggc gagggtcgac
     1081 atcatggcga tcttgtcgcg cgccttgaga gcgccgtcga tcacccggac gtaggtgatg
     1141 acgccccggt aggtgtcgta cacggagtcg aagatcatgg cgcgcgtggg cgcatccgga
     1201 tcgcccacgg gcggcggaat gtcccgcacc acgcggtcca acaacgcccc cacaccctgg
     1261 cccgtcttcc cgctcacacg cagcacgtcg tccacatcgc aaccgatgag gtgggcgagt
     1321 tccgcggcgt agcgatcggg gtcggccgac ggcaggtcga tcttgttcaa caccgggatg
     1381 atcgtgaggt cgttctccat cgccatgtag aggttggcga gggtctgtgc ctcgatgccc
     1441 tgcgcggcgt cgaccagcag caccgctccc tcacacgctg cgagtgagcg ggacacctcg
     1501 taggaaaagt cgacgtggcc gggcgtgtcg atcatgttga gcgcatgggg cttcccatcg
     1561 acgctccacg gcatgcgcac cgcttgcgac ttgatggtga tgccgcgcag ggcatcgagg
     1621 cacagactct cgccaacctc tacatggcga tggagaacga cctcacgatc atcccggtgc
     1681 tgaacaagat cgacctgccg tcggccgacc ccgatcgcta cgccgctgaa ctcgcacacc
     1741 tcatcggttg cgacgcggac gacgtgctgc gcgtgagcgg gaagactggc cagggcgtgg
     1801 gggagttgct cgaccgcgtg gtgcgggaca tcccgcagcc cgtcggcgat cccgacgcgc
     1861 ccacccgtgc catgatcttc gactccgtgt acgacaccta tcggggcgtc atcacgtacg
     1921 tccgggtgat cgacggcgct ctcaaggcgc gcgacaagat cgccatgatg tcgaccctcg
     1981 ccacccacga attgcttgag attggcgtca tcagccccga gccgacaccc accaagggcc
     2041 tccgcgtggg ggaagtgggc tacctcatta c
//
LOCUS       DASRKH010000213         2005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_14691, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000213 DASRKH010000000
VERSION     DASRKH010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14691"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="VFC06_03500"
     CDS             complement(<1..1033)
                     /locus_tag="VFC06_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80994.1"
                     /translation="MAGNHRVVALNLGTANIVAVQGLVDDAPQGKRTAVVEKIATMPT
                     PTGAVRANGVYNPAAITEALRELWRRDGLQTKNVIVGLDSRRIQSGLKWIDHYHPEDL
                     AYAAKAAWVNAQQILPSKVDAQTSTIHHYVLRERDIENKATGLTTSQILVLIIAVPTV
                     EAQALANIVEDTGMELVGMDLTALGMLRAANTPTYDDRRVVHMLVDIGETFVTMVLHN
                     NGNVRAIDTTSGSAGDSVSRAIGEELSRMSRDLGASTLDPTHIKRTLGEMVKSNAEDP
                     VTKRALAATVHQADGVFRDMDTFVSGYIRRVNETPAITAPGSAPAHNNEERGLMAIML
                     TGGGSILYGL"
     gene            1151..>2005
                     /locus_tag="VFC06_03505"
     CDS             1151..>2005
                     /locus_tag="VFC06_03505"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HZL80995.1"
                     /translation="MAAASESIQINPRFLVSIGALSETRLQQLTETHRLIEEDAKEKG
                     QQPRGMISWLLDEESVTMEQVMMAFAAMQGLSFFRLEDITPPRHVLDLIPHHIAREHR
                     IVPVSVNDNRVRVVIVNPTGRAKNALELAIGHIELEYAIATRNVIDTKVAEYYSAASE
                     AATIMIETEVTSGDMPSLDAIIEGSGSSDQGVSKNVRLLLTEGLQLGASDIHVDPYGK
                     GSRVRYRVDGQLRVSKDLVYPPEVARLIVSRIKVMANLDIAERRLPQDGRIELMVNGQ
                     PIDLRVSVL"
BASE COUNT          364 a          647 c          623 g          371 t
ORIGIN      
        1 cgagcccgta aaggatgctg ccgccgcctg tgagcatgat cgccatgaga ccgcgctctt
       61 cgttgttgtg tgccggcgcg ctgcctggag ccgtgatcgc aggcgtctca ttgactctgc
      121 ggatgtaacc ggacacgaac gtatccatgt cacggaacac gccgtcggcc tggtgcacgg
      181 tggcggccag agcgcgcttg gtgaccgggt cctcggcgtt gctcttgacc atttcgccca
      241 gggtccgctt gatgtgagtt gggtccagcg tggaagcgcc caggtccctt gacatccggc
      301 tgagttcctc gccgatcgcg cggctcacag agtcgccggc cgaaccggag gtcgtgtcga
      361 tagcgcggac gttgccgttg ttgtgcagca ccatcgtcac gaacgtctcg ccaatgtcta
      421 cgagcatgtg gacgacgcgg cggtcgtcgt aggtgggcgt gttcgcggcg cggagcatgc
      481 ccagcgccgt gaggtccatt cccacgagtt ccattccggt gtcttccaca atgttggcca
      541 aggcctgcgc ttcgacggtt gggacggcaa tgatcagcac caggatctgg ctcgtggtca
      601 agccggtcgc cttgttctcg atgtcacgct cgcgcaggac gtagtggtgg atggtggacg
      661 tctgagcgtc gaccttgctg ggcagaatct gctgtgcgtt cacccacgcc gccttcgccg
      721 cgtatgcgag gtcctcgggg tggtagtggt caatccactt cagccccgac tggatgcgtc
      781 gcgagtccag tccgacgatg acgttcttcg tttgcagccc gtcacggcgc cacaactccc
      841 tgagcgcctc ggtaatggcg gcggggttat agaccccgtt cgcacgcaca gcgcctgtcg
      901 gggtcggcat ggtcgcgatc ttctcgacca ctgcggtcct cttgccttgc ggggcgtcgt
      961 ccacgagacc ctgcaccgcg acgatgttcg ccgtgcccag gttcagggct acgacccgat
     1021 gattgccagc catgtccacc tccaaaggcc accacacttg gtagcaacgc aatactagag
     1081 caaaactgtg gcaggtttta cgctctacga tggacgccta ggcactgacg tgatcgcagg
     1141 agaggacaaa atggcagccg caagcgagag catccagatc aacccaaggt ttctggtgtc
     1201 gattggcgcg ctaagtgaga cccggctgca gcaattgacg gagactcacc gcctcatcga
     1261 agaggacgcg aaggagaagg gccagcagcc gcgcggcatg atctcgtggc tgctcgacga
     1321 agagtcagtc accatggagc aggtgatgat ggcgttcgcc gccatgcagg ggctctcgtt
     1381 cttccggctc gaggacatca cgccaccacg ccacgtgctg gacctaatcc cccaccacat
     1441 cgcccgcgaa caccgcatcg tgccggtgag cgtcaatgac aaccgggtgc gcgtagtcat
     1501 cgtcaacccg accggccggg ccaagaacgc gctcgaactg gccatcggcc acatcgaact
     1561 cgaatacgcg atcgccacac gcaacgtcat cgacacgaag gtcgccgagt actactctgc
     1621 ggcgtccgag gccgcgacca tcatgatcga aacggaggtc acgtccggcg acatgccgtc
     1681 gctcgacgcc atcatcgagg gttcgggctc ctccgaccaa ggcgtgtcca agaacgtgcg
     1741 cctgctgctc accgaggggc tgcagttggg cgcctccgac attcacgtcg acccctacgg
     1801 aaagggttcg cgcgtccggt atcgcgtcga cggccaactg cgagtgtcaa aggacctggt
     1861 ctacccgccc gaggtcgccc gactcatcgt cagccgcatc aaggtcatgg ccaacctcga
     1921 catcgccgaa cgccgactcc cgcaggacgg ccgcatcgag ttgatggtga acggccagcc
     1981 gatcgacctg cgcgtctccg tgctg
//
LOCUS       DASRKH010000214         4189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_25197, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000214 DASRKH010000000
VERSION     DASRKH010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25197"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VFC06_03510"
     CDS             complement(<1..365)
                     /locus_tag="VFC06_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80996.1"
                     /translation="MLSVASLLLLGVFVARLVDVQLINAAPLAEEALAQRLVTADVTP
                     ARADIVDRNGVVLATSVERYNVWVNQVKIATWKRTEQGKVLAEGPLDAARILAPILGI
                     NESELAADLVGDKTFEYLA"
     gene            complement(524..919)
                     /locus_tag="VFC06_03515"
     CDS             complement(524..919)
                     /locus_tag="VFC06_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL80997.1"
                     /translation="MSAAPLRSNARPAAAPTSAPQRATHLRAVTAPRHARSIAPFAWL
                     CIGIVLVALSSVLALNTTMAEGSYRSRDLKIEIAQLHQQRAAALIQLEANAAPAALAG
                     RATELGMQPAGRIGFVTLESGKVLEAGGQ"
     gene            complement(916..1887)
                     /gene="rsmH"
                     /locus_tag="VFC06_03520"
     CDS             complement(916..1887)
                     /gene="rsmH"
                     /locus_tag="VFC06_03520"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HZL80998.1"
                     /translation="MGTAGYRHTPVMLDRCVELLSPALEEPGSVAIDATLGMGGHTEA
                     LLAARPHARIVGIDRDADAIALASERLNGAGDRFVAHHATYDDIQGALARLGADRADA
                     ILFDLGVSSLQIDETDRGFTYARDAPLDMRMNRDDPVTAASLLATETEEELTRILHVY
                     GEERFARKIARSIVRRRETDPIERSEQLVDLVRACIPAATRTPGSNPAKRTFQALRIA
                     VNSELDVLEAALDAAIDAVRVGGRIVVLSYHSLEDRIVKHAFAAGTEVRVPPGMPVIP
                     DDAQPYLRLLTRGAEKASEAEIEVNPRAKSVRLRAVERTRVTPRRAP"
     gene            complement(1990..2454)
                     /gene="mraZ"
                     /locus_tag="VFC06_03525"
     CDS             complement(1990..2454)
                     /gene="mraZ"
                     /locus_tag="VFC06_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HZL80999.1"
                     /translation="MSELTHGLGPTGVFLGTFTPRLDDKGRLILPSKFRGRLASGLVA
                     TRGLDRCVFLFPVDEFMQVHERLRKAPLENKQARDYLRNFLGHAQDDVPDKQGRILLG
                     SPLRDYAALTRDVAVVGMGSRVEVWDAATWQNYLAEGEEAYSATAEALLNDM"
     gene            2649..3680
                     /locus_tag="VFC06_03530"
     CDS             2649..3680
                     /locus_tag="VFC06_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZL81000.1"
                     /translation="MSHPLPTEAELAEVAEITARMRESIGTVLAGLDQAVTATLAVVL
                     AEGHLLMEDVPGVGKTTLARALALSLDCTVGRLQFTPDLLPSDVTGVSIFRADDHQFE
                     FRPGPIFANIVIADEINRASPKTQAALLESMQERSVTVDGQTRPLPRPFVVVATQNPI
                     EMEGTYPLPEAQRDRFMAQVSVGYPSARDEMSMLDTHDGVDPLTLVRPVTDTETLARL
                     IAVARRVYAAPAIKQYIVDLVTATRNDSSLRLGASPRAALQLLAAAKARAAMAGRTHV
                     LPDDIKALAVAVLAHRLIPSTEARLAGKTAAKILSEAVARTPVPTEARDLPPHREQAV
                     SAETRIRAV"
     gene            3683..>4189
                     /locus_tag="VFC06_03535"
     CDS             3683..>4189
                     /locus_tag="VFC06_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81001.1"
                     /translation="MAPRPSSDTVLTSRGVGLAAAGVVVGALGVGLAAPPLVYVGVAM
                     LAAVAVGGLWLVLSINGFLRRFPFAHREVVPHPLTVGVPGRVTVTISSSSGSRGSALR
                     RALADSLDIREQAAAELTGGMSTRATVSRTAKAFSLSYSLLPARRTSTPRGDQGTGCH
                     GRPEPRSSR"
BASE COUNT          654 a         1469 c         1374 g          692 t
ORIGIN      
        1 ttggccaggt actcaaaggt cttgtcgccc acgaggtcgg ctgcgagctc tgactcgttg
       61 atgccgagaa tgggcgcgag gatccgcgcg gcatcgagcg gcccctccgc aagaaccttg
      121 ccctgctcgg tgcgcttcca cgtggcgatc ttgacctggt tgacccacac gttgtagcgc
      181 tcgaccgacg tcgccagcac taccccgttg cggtcaacta tgtcggcccg cgccggtgtc
      241 acgtccgcgg tgacgagccg ctgggcgagc gcctcctccg cgagcggcgc agcgttgatc
      301 agttgcacgt cgacgagtcg cgccacaaac acgccgagca gcagcagcga agcgacggaa
      361 agcaccgtct gtcgacggcc gggctcgcct acctttggtc cgccgggaac ctgcctccga
      421 ggactgcggg tgggtgcttg gcgtcggggg cgtggcgctc cgtcgcggcg ctccggagcg
      481 gcgcgggacg attgcggtcg gcgcctcgcg tcgtgggggg tcactactgg ccccccgcct
      541 caagcacctt gcccgattcg agcgtgacaa agccgatgcg gcccgccggc tgcattccca
      601 gttcagtggc acggcccgcg agggctgcgg gcgccgcatt cgcctcaagt tgaatcaacg
      661 cggccgcgcg ctgctggtga agctgcgcga tctcgatctt caggtcgcgg gagcggtagg
      721 agccttcagc catcgtcgtg ttgagtgcga gcacggacga cagcgccacg agcacaatgc
      781 cgatacatag ccaggcgaag ggcgcgatgc tgcgggcgtg gcggggcgcc gtcaccgctc
      841 gcaagtgagt ggcgcgctgc ggtgcggacg ttggcgcggc ggctgggcgc gcgttcgacc
      901 tcagcggcgc ggcgctcatg gcgccctcct gggcgtgacg cgtgtgcgtt ccacggcgcg
      961 caggcgcacc gacttggccc gagggttgac ttcgatctcc gcctcggagg ccttctcggc
     1021 ccccctggtg agcaagcgca agtacggctg ggcgtcgtcc ggaatgaccg gcatgcctgg
     1081 gggaaccctg acctcggtgc cggcggcgaa cgcgtgcttc acgatccggt cctcaagcga
     1141 gtgatagctg agcaccacaa tgcggccgcc gacgcgcacc gcatcgatcg ccgcgtcaag
     1201 tgccgcctcg aggacgtcca gttcgctgtt cacggcgatg cgcagtgcct ggaaagtccg
     1261 cttggcaggg ttgcttcccg gcgtgcgagt ggcggcgggg atacaggccc gcaccaggtc
     1321 aaccagttgt tcggagcgtt cgatcgggtc ggtctcgcgt cgccgcacga tcgagcgtgc
     1381 gatcttccga gcgaatcgct cctcgccgta gacgtgcaga attcgcgtca actcttcctc
     1441 ggtctcggtc gcgagcaaag aggcagccgt gacggggtcg tcgcggttca tgcgcatgtc
     1501 gagcggcgcg tcacgggcgt acgtgaagcc cctgtctgtc tcgtcgatct ggagcgagct
     1561 gacccccagg tcgaacagga tcgcgtcggc acggtccgca cccagcctcg cgagcgcgcc
     1621 ctggatgtcg tcgtacgtgg cgtgatgggc gacaaagcga tcccccgcgc cgttcagccg
     1681 ctcggacgcg agcgcgatgg cgtcggcgtc gcggtcaatg ccgacgatgc gcgcgtgggg
     1741 tctggccgcc agtagtgcct cggtgtgtcc gcccatcccc aacgtggcgt cgatggcgac
     1801 ggagccgggc tcttcgaggg caggcgagag caactcgacg caacggtcga gcatgaccgg
     1861 cgtgtgccgg tatcccgctg ttcccatgtg cccttcccgt cccgatgccg cgtcttgggg
     1921 tctccctccg aatctctgcc ggggggaagt ccgtcagagc gtttcgggag gaggccccac
     1981 gacgcggcgc tacatgtcgt tcagcagcgc ctcggcggta gccgaatacg cctcctcgcc
     2041 ttccgcgagg tagttctgcc aggtggccgc gtcccacact tcgacgcgcg accccattcc
     2101 caccactgcc acgtcgcggg tcagcgcggc gtagtccctc agcggggagc ccaacaaaat
     2161 gcgtccctgc ttgtccggca cgtcgtcctg ggcgtgaccc aggaagttgc gcaggtagtc
     2221 gcgcgcctgc ttgttctcga gcggtgcctt gcgcaggcgc tcgtgcacct gcatgaactc
     2281 gtccaccggg aacaggaaga cgcaccggtc gagaccacga gtggccacga ggcccgatgc
     2341 gagccttccc ctgaacttcg agggaaggat caatcggccc ttgtcgtcga gcctgggcgt
     2401 gaacgtgcca aggaagaccc cggtcggtcc tagaccgtgc gtcaattccg acaccacgac
     2461 tcacctcctc gcgccacgac tcgccagttc gccccacatt actccacttc tctccacaat
     2521 tttggcgaat atgacaaagt gcacttccct gagctgtatt acaccaggta aataaccaag
     2581 acacgcggtg gggggaccat gcacgagagt cttcgcttag gctcagggga gacatcgaag
     2641 gagcgttcat gagccacccc ttgcccaccg aggccgaact cgcagaggtc gccgagatca
     2701 cagcgcgcat gcgcgagtcg atcggaacgg tgctcgcggg cctcgatcag gcggtcaccg
     2761 caacgctcgc ggtcgtgctc gccgagggac acctcctcat ggaggacgtg ccaggcgtcg
     2821 gaaagacgac gctggcaagg gccctcgcgc tgagcctcga ctgcaccgtg ggaaggctgc
     2881 agttcacgcc cgacctgctg ccgtcggacg tgaccggcgt gagcatcttt cgggcggacg
     2941 accatcagtt cgagttccgc ccgggcccga tcttcgccaa catcgtcatc gccgacgaga
     3001 tcaaccgagc aagccccaag acccaagccg cactcctcga gtccatgcag gagcgctccg
     3061 tgaccgtcga cgggcagact cgtccactcc cccgaccctt cgtcgtcgtc gccacccaga
     3121 acccgatcga gatggagggg acctatcccc tgcccgaggc ccagcgcgat cgcttcatgg
     3181 cccaggtgtc cgtcggctac ccgtccgcgc gcgacgagat gtcgatgctc gacactcacg
     3241 atggagtcga cccgttgacg ctggtgcgtc ccgtcaccga cacggagacc ctcgcgcgcc
     3301 tcatcgcggt agcccgccgg gtgtatgcgg ctcccgcgat caagcagtac atcgtggatc
     3361 tggtgacggc caccagaaac gactcttcgc tacgactcgg cgcgtctccg cgcgccgcgc
     3421 ttcagctact cgcggcggcc aaggcgcggg ctgcgatggc cggccggacc cacgttcttc
     3481 ccgacgacat caaggcgctc gcggtggcgg tgctggccca ccgcctcatc ccgagcaccg
     3541 aggcgcgcct cgcgggcaag accgccgcga agattctcag cgaagcggtc gcccgcacgc
     3601 ctgtgcccac cgaggcgcgc gatcttccgc cgcatcgcga acaggcggtg tccgccgaaa
     3661 cacgcatccg agccgtctag cgatggctcc acgccccagt tccgacacgg tgctcacgtc
     3721 acggggcgtc ggcctcgccg ccgcgggcgt cgtcgtcggc gccttgggcg tcggactcgc
     3781 cgcaccgccc ctcgtgtacg tcggagtcgc gatgctggcc gccgtcgctg tgggcgggct
     3841 gtggctcgta ctctccatca acgggtttct caggcgcttc ccgttcgcgc accgcgaggt
     3901 ggtaccgcac cccctcacgg tgggcgtgcc cggtcgcgtc accgtcacca tcagctcctc
     3961 atcggggagt cgcggcagtg ccctgagaag ggcgctcgcc gattcactcg atatccggga
     4021 acaggcggcc gccgaactca ccggcggaat gagcaccagg gccacggtga gccgcaccgc
     4081 caaggccttc tcgctgtcct attcgctcct tcccgcgcgc cgcacttcaa ctcctcgcgg
     4141 cgaccaaggc acgggctgcc atggccggcc ggaaccacgt tcttcccga
//
LOCUS       DASRKH010000215         2086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_367, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000215 DASRKH010000000
VERSION     DASRKH010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="VFC06_03540"
     CDS             complement(<1..709)
                     /locus_tag="VFC06_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81002.1"
                     /translation="MAILSIGAPRRAGAVSLEQLLANELNPLRATLDSLNANCFIAGL
                     DLTLVYRNRKANQTLQDLGPAIRQAFGLSVDELLGGSIHRFHKDPARVERILADPKAL
                     PRAATFSFGGITLRTLISAITDSAGTRHGYVVIWDNVSERNSAADSAFLSVESATGVL
                     QEITQRIDRVAAMTSEQATTAAAATEQLRAAVAEIARSSNDASEQSTQAVAATVDGLG
                     GLLTGVVGGTGDLSHGCA"
     gene            complement(877..1965)
                     /locus_tag="VFC06_03545"
     CDS             complement(877..1965)
                     /locus_tag="VFC06_03545"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HZL81003.1"
                     /translation="MDTPRPVTEQTLPHDVLVALSNSRDLLSTWWPAAVVLGLAVSLV
                     VVGVLRRHRGRRAWPVHTAAGIAVVLAATLGVNTWVGYVPSVTAAALLLTEHESVGTA
                     SNGAVAPVAIAAPAALRMPTSTTWVYTPPGYDPNGSTRYPVVYLVHGTPGTSADWMSG
                     GDIAHVMDVLIAHHLVRPMIVVAPDVNGVGRADTECLDSTRGGSQVETYLGRVVVPWV
                     DAHYATAADWSHRAIGGMSSGGFCALDQGLRHPELYGAIIALEGYDNPGSGGRAMLAT
                     SAEITAHSPGKYLPTTTFEHPVAVFLSVGGAARGGDASEASGLAAQLTARGQSVLFRI
                     EPGQNHTWTMARTAIPYGLELVSAALAP"
BASE COUNT          319 a          692 c          768 g          307 t
ORIGIN      
        1 gcgcgcagcc gtggctgaga tcgcccgttc ctccaacgac gccagtgagc agtccaccca
       61 ggccgtcgac ggtggcagcg acggcctggg tggactgctc actggcgtcg ttggaggaac
      121 gggcgatctc agccacggct gcgcgcagct gctcggtggc tgctgctgcg gtcgtcgcct
      181 gctcggacgt catggcggcc acccggtcga tacgctgggt gatctcctgc agcacgccgg
      241 tcgccgactc gacgctcagg aaggccgagt cggcggcgga gttgcgctct gagacgttgt
      301 cccagatcac gacatagcca tggcgggtgc ccgccgaatc cgtgatcgcg ctgatcaggg
      361 tccggagagt gatcccaccg aacgagaacg tcgccgccct cggtagagcc ttggggtcgg
      421 ccaagatgcg ctcgacccgg gctgggtcct tgtggaagcg atggatcgag ccgccgagca
      481 gctcgtccac gctcaggccg aacgcctggc ggatggcggg gccgaggtcc tgaagggtct
      541 gattcgcctt acggttccga tagaccagtg tcaggtccag acccgcgatg aagcagttgg
      601 cgttcagact gtccaacgtc gcgcgcaacg ggttcagctc gttggcgagc agctgctcca
      661 gcgaaaccgc cccagcccgg cgaggtgcgc cgatcgacaa gattgccatg ttagctgtcc
      721 ttctcgtggc agaacggtgc ggtccgcgct ccaatcggca gtccggtcgc accatgaagg
      781 atttgacccc gacgaagacc acgtcgtctt gtgcggcagc gggtgacccg tgacccgcga
      841 ccgcgacacc tgcaaggccc ggaacgtcgt cgaacgctac ggcgcgagcg cggccgagac
      901 gagctcgagg ccgtacggga tcgcggtgcg ggccatcgtc caggtgtggt tctggcccgg
      961 ttcgatgcgg aacagcacgg actggccgcg cgcggtgagc tgcgcggcca gtccgctggc
     1021 ctcggacgcg tcgccaccgc gcgcagcacc gcccacgctg aggaacaccg cgacggggtg
     1081 ctcgaacgtc gtcgtgggca ggtacttccc gggggagtgc gccgtgatct cggcgctcgt
     1141 cgcgagcatc gcgcgccccc cgctgccggg gttgtcgtag ccctcgagcg cgatgatcgc
     1201 cccgtacagc tcagggtggc gcagaccctg gtcgagcgcg cagaagccgc ccgacgacat
     1261 cccgccgatc gcgcggtggc tccagtcggc cgccgtcgcg tagtgggcgt cgacccacgg
     1321 cacgacgacc cggcccaggt acgtctcgac ctgcgagccg ccccgggtcg agtcgagaca
     1381 ttcggtgtcc gcccggccga cgccgttcac gtcgggagcg acgacgatca tcggacgcac
     1441 gaggtggtgc gcgatgagca cgtccatgac gtgggcgatg tcgccgccgg acatccagtc
     1501 ggccgacgtg cccggcgtgc cgtggacgag gtagacgacg gggtagcgcg tggacccgtt
     1561 cgggtcgtag ccgggcggtg tgtagaccca cgtcgtcgag gtgggcatgc gcagtgcggc
     1621 cggggcggcg atggccacgg gggcgacggc gccgttcgac gccgttccga cggactcgtg
     1681 ctcggtgagc agcagcgcgg ccgcggtgac cgatgggacg tacccgaccc aggtgttgac
     1741 cccgagggtc gccgcgagga ccacggcgat ccctgccgcg gtgtgcacgg gccacgcgcg
     1801 ccgtccccgg tgacgccgca gcacccccac gacgacgagc gacaccgcga gcccgaggac
     1861 gacggcagcc ggccaccacg tggacaggag atcgcggctg ttgctgaggg cgacgagcac
     1921 gtcgtgcggg agcgtctgct ccgtgaccgg gcgaggggtg tccacgccac cattggctcc
     1981 ccatgtcatg ggagcggccg gtgaggttca ggggagattc atgggagttg tgctcgcgtg
     2041 acccgtgagt gccgcgaacg acgtgggtga gcgcgatgat gagcgc
//
LOCUS       DASRKH010000216         2611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_6741, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000216 DASRKH010000000
VERSION     DASRKH010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6741"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VFC06_03550"
     CDS             complement(<1..378)
                     /locus_tag="VFC06_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HZL81004.1"
                     /translation="MKRDAARAGGRAHRDNVTSLSLYDETAQAAAAAVLDKYSTSFGA
                     GTRLLGRQMRTHIRSIYAMVRVADEIVDTYRGDDAGAMLDGFEREVNAALDSTFSANL
                     VAHAFAVSARHVGIGRNLTEPFFA"
     gene            complement(375..1457)
                     /locus_tag="VFC06_03555"
     CDS             complement(375..1457)
                     /locus_tag="VFC06_03555"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HZL81005.1"
                     /translation="MSGLLTTDALQEFVSSRLDAVQATLDDHVITATAELPHAGSHYR
                     DLWQTLGNQFGGGKNLRPSLTLAAYAGLGGEDDDAILPVAAAMEMLHIAMLVHDDVLD
                     HDEVRRGRPNVAGTRRAELAGSRLSPTRVDDHVMAAALLGGDLALASAYDLISRAPLP
                     AHHRLACLDLITRAIRTTVAGELLDMYGDLIDPANASSLLVAELKTATYSCVVPLRAG
                     AQLAGADPTMVNHMERLGASLGLSFQLVDDDLGVFGDPAVTGKSVLSDLRAGKRTELL
                     RLGFHNTDEAGREILRASVGNPDLDEQGAAAVRKVLVESGARERALKVARQHARTARR
                     IASERISAPLSGFLIGLIDSLEERSS"
     gene            complement(1635..>2611)
                     /locus_tag="VFC06_03560"
     CDS             complement(1635..>2611)
                     /locus_tag="VFC06_03560"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HZL81006.1"
                     /translation="RLLIQIGIAAAALIGLILVVIALFSLFNSGTREPEPTATLVTIP
                     ILEGVEQASAETRLTALELTPIFQEEMSADIAAGRVIRTDPPAGEQVDPLTNPEVTVF
                     ISTGPDEITVPEVRGLEREAAITELENAGLVISTFETDHDPDIAADRATKTEPAAGQV
                     VTPDTEVILYISDGKVELPELRNGTESEAQQILVALGLIVNLQDVETTGANPGTVVEQ
                     DPLPGLVDQGTTVTIKVAKAPTTVEVPDVVGKTKAEAQTLLSNAGLNVKVSEEPSLTV
                     AEGKVISQNPSAGIKVAIGTTVTITVSSGPVPETTGPAPPGPPGPPAR"
BASE COUNT          405 a          847 c          909 g          450 t
ORIGIN      
        1 ggcgaagaac ggctcggtga gattccggcc gatgccgacg tggcgggcgc tcacggcgaa
       61 ggcatgcgcc acgaggttgg cgctgaaggt gctgtcgagc gcggcgttga cctcgcgctc
      121 gaagccgtcg agcatggcgc cagcgtcgtc gccgcggtag gtgtccacga tctcgtccgc
      181 gacgcgcacc atcgcgtaga tcgagcgaat gtgggtccgc atctggcggc cgagcagcct
      241 ggtgcccgcc ccgaaggacg tggagtactt gtccagcacg gccgccgccg cggcctgggc
      301 ggtttcgtcg tacaggctca gggaggtgac gttgtcgcgg tgggcgcggc ctccggcgcg
      361 ggcggcgtcc cgcttcacga cgaacgctct tccaacgagt cgatcaggcc gatgaggaag
      421 ccggacaggg gcgcggaaat ccgctccgac gcgatgcgcc tggcggtgcg cgcgtgctgg
      481 cgcgccactt tgagcgcgcg ttcgcgggct ccggactcca ccagcacttt gcgcactgcg
      541 gcggcgccct gttcatcgag atccggattg cccacggagg cgcgcaggat ctcgcgccca
      601 gcttcgtccg tgttgtggaa gccaagccgc agcagttcgg tgcgcttgcc ggcgcgcagg
      661 tcggatagca ccgacttgcc cgtcacggca ggatccccaa acacgccaag gtcatcgtcc
      721 accaactgga acgacagccc cagggaggcc cccagcctct ccatgtggtt caccatcgtg
      781 gggtcggcgc cagcgagttg cgcgcccgcg cgaagtggca ccacgcagga gtaggtggcg
      841 gtcttgagtt ccgcgaccag cagggaactg gcgttggcag ggtcgatcag gtcgccgtac
      901 atgtcgagga gctcgcccgc gaccgtggtg cggatcgcac gggtgatgag atcgaggcac
      961 gcgaggcggt ggtgcgctgg cagaggcgcc cgggagatca ggtcgtatgc ggaggccaac
     1021 gcgaggtcgc cgcccagcag tgcggccgcc atcacgtggt cgtccactcg ggtgggactc
     1081 agtcgcgagc cggcgagctc cgctcggcgg gtgcccgcaa cgttgggtcg gccccggcgc
     1141 acctcgtcgt ggtcgagaac atcgtcgtgg accagcatgg cgatgtggag catttccatg
     1201 gccgcggcca cgggcaggat cgcgtcgtcg tcctcgccgc ccagccccgc gtaggccgcg
     1261 agagtgaggg aggggcgcag gttcttgccc ccgccaaact ggttgccgag cgtctgccac
     1321 aggtcgcggt aatgagaacc cgcgtgcggc aactctgccg tggccgtgat gacgtgatca
     1381 tcgagcgttg cctgcaccgc gtcgaggcgc gaggacacga actcttgcag ggcgtcggtg
     1441 gtcagcagtc cgctcatagt gcctgataag acaccggcgg acgcaaagtc attcccgaac
     1501 tggccaacaa tgtggccgct cgcgtctgct ccggtgactc tcgctcccat gacggcatgg
     1561 acggctcccc cgctatgcca ctcatgctac gcgtgcatcg tccctgctcg cggcaggaaa
     1621 ggcgcccgcc gaggctaccg tgccggcggt ccgggcggtc cgggcggtgc gggaccggtc
     1681 gtttcgggta ctggccccga cgacaccgta atggtcaccg tcgtgccgat cgcgaccttg
     1741 attccggcag acgggttctg cgaaatgacc ttcccttcgg ccaccgtcaa ggagggctct
     1801 tcgctcacct tgacgttgag ccccgcgttg gacaacaaag tctgcgcctc cgccttcgtc
     1861 ttgccgacca cgtcgggaac ctcgacggtg gtgggggcct tggcgacctt gatcgtgacc
     1921 gtcgttccct ggtcgaccag gcccggcagc gggtcctgct ccaccacggt gccggggttg
     1981 gccccggtag tctccacgtc ctgcaggttg acgatcaggc caagcgctac gagtatctgt
     2041 tgagcctcag actcggtgcc gttgcgcagt tccggcagtt cgaccttgcc gtccgaaatg
     2101 tacaggatga cctcggtgtc gggcgtcacg acttgtccgg cagcgggctc cgtcttggtg
     2161 gcgcggtccg ccgcgatgtc gggatcatgg tcggtctcga aggtgctgat caccagacca
     2221 gcattctcaa gttcggtgat cgcggcctcg cgctcgaggc cacgcacctc gggcacggtg
     2281 atctcgtccg gtccggtcga tatgaagacg gtcacctcgg gattggtaag cgggtcgact
     2341 tgctcaccgg cgggcggatc ggtacgaatc acgcgaccag ccgcgatatc agcggacatc
     2401 tcctcttgga agatgggcgt cagttcgagc gccgtcaggc gcgtctcggc acttgcctgt
     2461 tcgacgccct caagtatggg gattgtcacg agcgttgcgg tcggttccgg ctccctggtc
     2521 cccgaattga acagcgagaa cagggcgatc accacgagga tgaggccgat cagcgcggca
     2581 gcggcgatcc cgatctggat caggagccga c
//
LOCUS       DASRKH010000217         2124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_41673, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000217 DASRKH010000000
VERSION     DASRKH010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41673"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VFC06_03565"
     CDS             <1..268
                     /locus_tag="VFC06_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019134872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HZL81007.1"
                     /translation="GKLDDVPIVDVRRFETELLDHLRRNTDVLTTIATSGKLEAATEA
                     ALDAGVDDFRNGFLKGDGTPLVGAVDADEAVQIDQEQIVRQKKA"
     gene            271..1164
                     /locus_tag="VFC06_03570"
     CDS             271..1164
                     /locus_tag="VFC06_03570"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019134871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HZL81008.1"
                     /translation="MAGTQRVYKQRIKSTQALKKMFRAQELIAASRIGRARDRMHHAM
                     PASRAITHAVSALATHSTVSHPLLSQRADTNRVAVLLVASDRGMAGAYSASVIREAER
                     LVERLEAAGKEVALYVVGRRAVSYYSFRRRPLTGQWTGGSDSPAVEVADEVAGTLLAA
                     FQAPADEGGVSELHVVYTQFVNMVTQRPRVIRMLPLEVVEGVAPAGTDDVLPLYEFEP
                     SPEEVLDALLPRYVRSRIYTCLLQAASSELAARQRAMHTATQNAEDLIRLYTRLANQA
                     RQSEITQEISEIVSGADALAS"
     gene            1249..1989
                     /locus_tag="VFC06_03575"
                     /pseudo
     CDS             1249..1989
                     /locus_tag="VFC06_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116311.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
     gene            complement(1978..2124)
                     /locus_tag="VFC06_03580"
                     /pseudo
     CDS             complement(1978..2124)
                     /locus_tag="VFC06_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792927.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; incomplete; partial in the middle of a
                     contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
BASE COUNT          357 a          752 c          719 g          296 t
ORIGIN      
        1 gggcaagctc gacgacgtcc cgatcgtcga cgtccggcgc ttcgagaccg agctcctcga
       61 ccacctgcgc cgcaacaccg acgtgctgac gacgatcgcg acgtcgggca agctcgaggc
      121 cgcgaccgag gcggcactgg acgcgggggt cgacgacttc cgcaacggct tcctcaaggg
      181 cgacgggaca ccgctcgtcg gtgccgtcga tgccgacgag gcagtgcaga tcgaccagga
      241 gcagatcgtc cggcagaaga aggcctgagg atggccggta cccagcgcgt ctacaagcag
      301 cggatcaagt cgacccaggc gctgaagaag atgttccgcg cccaggagct catcgctgcg
      361 tcgcggatcg gtcgtgcgcg cgaccgcatg caccacgcga tgccggcatc gcgtgcgatc
      421 acgcacgccg tctcggcgct cgcgacgcac tccacggtgt cgcacccgct gctctcgcag
      481 cgcgccgata cgaaccgggt cgcggtgctg ctggtcgcgt ccgaccgcgg gatggcgggc
      541 gcgtactccg cgagcgtcat ccgcgaggcc gaacggctgg tcgagcggct cgaggccgcc
      601 ggcaaggagg tcgcgctgta cgtggtcggg cgccgcgcgg tctcctacta ctcgttccgc
      661 cggcgtccgc tgacaggtca gtggacgggc gggtcggact cgcctgctgt cgaggtggct
      721 gacgaggtcg cgggcacgct gctcgcggcc ttccaggctc ccgcggacga gggcggcgtg
      781 agcgagctgc atgtcgtgta cacgcagttc gtcaacatgg tgacccaacg cccgcgcgtg
      841 atccggatgc tgccgctcga ggtcgtcgag ggcgtcgcac cggcgggcac ggacgacgtg
      901 ctcccgctct acgagttcga gcccagccca gaggaagtgc tcgacgcgct gctcccgcgc
      961 tacgtgcgca gccgcatcta cacctgcctg ctgcaggccg catcgtccga gctcgcggcc
     1021 cggcagcgtg ccatgcacac cgcgacccag aacgccgagg acctcatccg cctgtacacc
     1081 aggctggcga accaggcccg gcagtccgag atcactcagg agatcagcga gatcgtgtcg
     1141 ggtgccgacg cgctcgcctc ctgacccaca gccagacagc accacagcag gaccgcacga
     1201 cccagaccac gccggagtcc gctccggtcg agcgaagcga ggcaggacat gaccgccacc
     1261 accgtcgacg gcacgccgac caactcgggc acgcccggag tcggccgcgt cgcccgggtg
     1321 atcggccccg tcgtggacat cgagttcccg tcggaccaga tccccgagat gtacaacgcc
     1381 ctcacggtca agatcgacct gtcgtcgcag ggcgaggacg agaagtcctt cacgatgacc
     1441 ctcgaggtcg cccagcacct cggcgactcg ctcgtgcggg cgatcgcgct caagccgacc
     1501 gacggcctgg ttcgtggcgc gctcgtgacc gacaccggcg cgccgatcag cgtcccggtc
     1561 ggggacatca ccaaggggca cgtgttcaac gtgaccggtg acgtcctcaa cctggcgccc
     1621 ggcgagaagc tcgagatcac cgagcgctgg ccgatccacc gcaagccccc ggccttcgac
     1681 cagctcgagt ccaagaccca gatgttcgag acgggcatca aggtcatcga cctgctgacg
     1741 ccgtacgtgc tcggcggcaa gatcggcctg ttcggcggtg cgggcgtcgg caagacggtc
     1801 ctcatccagg agatgatcca gcgcgtcgca caggaccacg gcggcgtctc ggtgttcgcc
     1861 ggtgtcggtg agcgcacccg tgagggcaac gacctcatcg ccgagatgga ggaggcgggc
     1921 gtcttcgaca agactgcgct cgtcttcggg cagatggacg agccgccggg cacgcgtctg
     1981 cacgtcgcgg aagtactccg ccatcgtcag ggccgacagt gcgacgcgca gacgcgtgcc
     2041 cggcggctcg tccatctgcc cgaagacgag cgcagtcttg tcgaagacgc ccgcctcctc
     2101 catctcggcg atgaggtcgt tgcc
//
LOCUS       DASRKH010000218         2114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_40643, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000218 DASRKH010000000
VERSION     DASRKH010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40643"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..928)
                     /gene="dapF"
                     /locus_tag="VFC06_03585"
     CDS             complement(<1..928)
                     /gene="dapF"
                     /locus_tag="VFC06_03585"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HZL81009.1"
                     /translation="MTGAPSPLAVERATTLHVTKGHGTENDFVLLDDRDGGLDLSATL
                     VRALTDRHAGVGGDGVIRLVESSRVPEGEAVLRADASARWFMDYRNADGSVAEMCGNG
                     VRVFAAYLERLGLWDPFGGELAVGTRAGVRWVRRIPAVAGFASVDDGPWYAVDMGPWS
                     LPGGPRAVEAGYDAEVAVVGLDDVRPALGVDVGNPHTVLALAGETELAAADLTRAPVV
                     VPVPPHGTNVELVVALGEQVGPDGAVVGRVRMRVHERGVGETRSCGTGACAAALAVRA
                     WAGDGAPDVWLVDVPGGTVRVSVLAGGHVELAG"
     gene            complement(925..1881)
                     /gene="miaA"
                     /locus_tag="VFC06_03590"
     CDS             complement(925..1881)
                     /gene="miaA"
                     /locus_tag="VFC06_03590"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HZL81010.1"
                     /translation="MSLVIAVVGPTATGKSDLGLALAEQLGGEIVNADAMQLYRGMDI
                     GTAKLTLAERRGIPHHQLDVLDVDQDASVADYQRGARADLAAIDVRGARAVAVGGSGL
                     YVRALLDHMDFPGTDPDLRARLEERVEVEGSRALHAELERVDPVAAAGIGPRNARRIV
                     RALEVIGLTGRPYSASLPQRVYEVPALQLGLDCDRAVLDARIEVRVGHMWDAGLVEEV
                     RALATHGIGRTASRAVGYAEVLAVLRGELSEAEARAAVAAGTRRLARKQMGWFGRDPC
                     VHWLDARDPSLVGRALDLVAAADAGTLGPATADPSPRRSLGS"
     gene            complement(1878..>2114)
                     /locus_tag="VFC06_03595"
     CDS             complement(1878..>2114)
                     /locus_tag="VFC06_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81011.1"
                     /translation="VLADLADGGPAARARLGDLDGAVAGELAVTGWGPWQVLLGAVGA
                     GEVDAAVLAAGAPFGAQHVVATWVPRTTRAGERT"
BASE COUNT          279 a          860 c          740 g          235 t
ORIGIN      
        1 gtccggcgag ctcgacgtgc ccgccggcca ggacgctcac gcggaccgtg ccgcccggca
       61 cgtcgacgag ccagacgtcg ggtgcaccgt cgcccgccca cgccctcacc gcgagcgccg
      121 cggcgcacgc gccggtcccg cacgagcgcg tctctccgac cccgcgctcg tgcacgcgca
      181 tccgcacacg acccacgacc gcgccgtcgg gaccgacctg ctcgccgagc gccacgacca
      241 gctcgacgtt cgtgccgtgg ggcggcaccg ggaccacgac cggcgcacgc gtgaggtccg
      301 ccgccgcgag ctccgtctcc cctgccaggg cgagcaccgt gtgcgggttg ccgacatcga
      361 ctccgagcgc cgggcgcacg tcgtcgagcc ccaccaccgc gacctcggcg tcgtagcccg
      421 cctcgaccgc ccgggggccg cccgggaggc tccacgggcc catgtcgacc gcgtaccacg
      481 ggccgtcgtc cacgctcgcg aacccggcca ccgccgggat ccggcggacc cagcgcacac
      541 ccgcacgcgt gccgaccgcg agctcgccgc cgaaggggtc ccacagcccg agccgctcga
      601 ggtaggcagc gaagacccgc actccgttgc cgcacatctc cgcgacggaa ccgtccgcgt
      661 tgcggtagtc catgaaccag cgcgcggacg cgtcggccct gagcaccgcc tcgccctcgg
      721 ggacccgcga gctctcgacg agccggatga cgccgtcgcc cccgaccccg gcatgccggt
      781 ccgtgagcgc gcgcacgagc gtcgccgaca ggtcgagccc accgtcgcgg tcgtcgagca
      841 gaacgaagtc gttctcggtg ccgtggccct tggtcacgtg cagggtggtg gcccgttcga
      901 cggccagggg gctcggtgcg ccggtcatga gcccagacta cggcgcggcg aagggtcggc
      961 cgtggcgggt ccgagggtgc ccgcgtcggc cgcagccacg agatcgagcg cgcggccgac
     1021 cagggacggg tcgcgcgcat cgagccagtg cacgcagggg tcccgcccga accagcccat
     1081 ctgcttgcgc gcgagccgcc gggtcccagc cgcaactgcc gcacgcgcct cggcctcact
     1141 cagctcaccg cgaagcaccg cgagcacctc ggcgtagccg accgcacgcg acgcggtgcg
     1201 cccgatgccg tgcgtggcga gcgcccggac ctcctcgacg agcccggcgt cccacatgtg
     1261 ccccacacgc acctcgatcc gtgcgtcgag caccgcacgg tcgcagtcga ggccgagctg
     1321 cagtgccggc acctcgtaga cgcgctgcgg gaggctcgcc gagtacggcc ggcccgtgag
     1381 cccgatgacc tcgagcgccc gcacgatgcg ccgcgcgttg cgaggaccga tccccgccgc
     1441 tgcaaccggg tcgacacgtt cgagctcggc gtgcagcgcg cgcgatccct cgacctcgac
     1501 ccgctcctcg agccgcgcgc gcaggtccgg gtcggtaccg gggaagtcca tgtggtcgag
     1561 cagcgcgcgc acgtagaggc ccgagccgcc caccgcgacg gcccgcgcac cccgcacgtc
     1621 gatcgcggcg aggtcggccc gcgctccgcg ctggtagtcg gcgaccgagg cgtcctggtc
     1681 cacgtcgagc acgtcgagct ggtggtgcgg gatgccgcgt cgctcggcga gcgtgagctt
     1741 cgcggtcccg atgtccatgc cccggtagag ctgcatcgcg tcggcgttca cgatctcgcc
     1801 gccgagctgc tcggcgagcg cgaggccgag gtccgacttg ccggtcgccg tggggccgac
     1861 gaccgcgatc acgaggctca tgtccgctca cccgcgcgcg tcgttcgcgg gacccaggtc
     1921 gccacgacgt gctgggcgcc gaagggtgcg cccgccgcga ggaccgccgc gtcgacctcg
     1981 cccgctccga ctgcaccgag cagcacctgc cagggacccc acccggtcac cgcgagctcg
     2041 cccgcgacag ccccgtcgag gtcgccgagc cgcgcgcggg ccgccgggcc gccgtcggcc
     2101 aggtccgcga gcac
//
LOCUS       DASRKH010000219         2126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_53489, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000219 DASRKH010000000
VERSION     DASRKH010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53489"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VFC06_03600"
     CDS             <1..355
                     /locus_tag="VFC06_03600"
                     /inference="COORDINATES: protein motif:HMM:NF014864.4"
                     /note="regulates arginine biosynthesis when complexed with
                     arginine by binding at site that overlap the promotors of
                     the arginine biosynthesis genes; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HZL81012.1"
                     /translation="LSRDLLDIGALKVRGASGFMVYTTQASLEASGDTDGRLNRLCAE
                     LLYSADGSGNIAILRTPSGGAQYFASGIDGHDDPDVVGTVAGDDTVFVISRDVEGGPK
                     LAQKFLDRASGVVHD"
     gene            380..1582
                     /locus_tag="VFC06_03605"
     CDS             380..1582
                     /locus_tag="VFC06_03605"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015771401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HZL81013.1"
                     /translation="MTERIVLAYSGGLDTSVAIGWIADATGAEVVAVAVDVGQGGEDL
                     EVIRQRALDCGAVEAYVADARDEFAEEYCMPALKANALYHDKYPLVSALSRPVIVKHI
                     VQAAREFGATTMAHGCTGKGNDQVRFEVGIASLAPDLKCIAPVRDLALTRDKAIDYAE
                     RNNLPIATTKKNPFSIDQNVWGRAVETGFLEDIWNAPTKDVYEYTDDPSFPPVPDEVI
                     ISFEKGIPVALDGQKVTPLQAIQELNRRAGAHGVGRIDIVEDRLVGIKSREVYEAPGA
                     IALIEAHREMENVTLEREQARYKRVIQQQWTDLVYDGMWSSPLKKSLDAFIEDTQQYV
                     TGDIRMVLHGGKATITGRRSDIGLYDFNLATYDEGDTFDQSAARGFIEIYGLAAKQAA
                     ARASRAGL"
     gene            1660..>2126
                     /locus_tag="VFC06_03610"
     CDS             1660..>2126
                     /locus_tag="VFC06_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018135604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HZL81014.1"
                     /translation="MAKAEGTNEGALWGGRFSGGPAEALAKLSVSTHFDWRYADDDLA
                     ASIAHAHALQRASLLTDAETAKMVDALTALRVDVAAGKVLPSEGDEDVHGALERLLIE
                     RIGPDVGGKLRAGRSRNDQIATLPRMYLRRHARIIAAAVLDVVDALLEQADRH"
BASE COUNT          382 a          723 c          706 g          315 t
ORIGIN      
        1 cctgtcgcgc gacctgctcg acatcggcgc gctgaaggtg cggggagcat cgggcttcat
       61 ggtctacacg acgcaggcct cgctcgaagc gtctggcgac acggacggtc gcctcaatcg
      121 cctgtgcgcc gagctgctgt actccgctga cggctccgga aacatcgcca ttcttcgcac
      181 gccgtccggg ggcgcccagt acttcgcctc cgggattgac ggtcacgacg accccgacgt
      241 cgtcggcacc gtggccggag acgacaccgt gtttgttatt tccagggacg tcgaaggcgg
      301 cccgaaactt gcccagaagt tcctcgatcg cgcctctggc gtcgttcacg actgacccat
      361 caggaaagag actcatcaca tgaccgaacg cattgtcctg gcatactcag gcggccttga
      421 cacctccgtc gcgatcggct ggatcgcgga cgcgaccggc gcagaggtgg tggccgtcgc
      481 ggtggacgtg ggccagggag gcgaagacct cgaggtgatt cgccagcgcg cgctcgactg
      541 cggcgccgtg gaggcgtatg tggccgacgc tcgcgacgag ttcgcggagg agtactgcat
      601 gcccgcgctc aaggcgaatg cgctctacca cgacaagtac ccgctcgtct cggcgctcag
      661 ccgacccgtc atcgtcaagc acatcgtgca ggcggcgcgc gaattcggcg ccaccaccat
      721 ggctcacggc tgcaccggca agggcaacga ccaggtgcgc ttcgaggtgg gaatcgcctc
      781 gctcgcgccg gacctcaagt gcatcgcgcc cgtgcgcgac ctcgccctga cgcgcgacaa
      841 ggccatcgac tacgccgagc gcaacaacct gcccatcgcg accaccaaga agaacccctt
      901 ctcgatcgac cagaacgtgt ggggccgcgc cgtggagacg ggcttcttgg aggacatctg
      961 gaacgccccc accaaggacg tgtacgagta cacggacgac ccctccttcc cgccggtccc
     1021 cgacgaggtc atcatctcgt tcgagaaggg catccccgtc gcgctggacg gccagaaggt
     1081 cacgccgctg caggcgatcc aggagctgaa ccgtagggcc ggagcacacg gagtgggccg
     1141 catcgacatc gtcgaggacc gcctggtggg catcaagagc cgcgaggtct acgaggcgcc
     1201 cggcgcgatt gcgctgatcg aggcgcaccg cgagatggag aacgtcacgc ttgagcgcga
     1261 gcaggcacgc tacaagcgcg tcatccagca gcagtggacc gacctggtct acgacggcat
     1321 gtggtcctcg cccctcaaga agtcgctcga cgcgttcatc gaggacacgc agcagtacgt
     1381 caccggggac atccgcatgg tgttgcacgg cggcaaggcg accatcacgg gacgccgctc
     1441 cgacatcggc ctgtacgact tcaaccttgc cacgtacgac gagggtgaca ccttcgatca
     1501 gagcgcggcc cgcggattca tcgagatcta cgggctggcg gccaagcagg ccgccgctcg
     1561 cgcctccagg gcaggcctct agcagccagt atcgggaacc cgccgcaccc cacggggcgg
     1621 cgggttcctg gcacacttgt gatggacgaa gggaccgcga tggcaaaggc cgaaggcacc
     1681 aacgagggcg cactgtgggg tggccgcttc agcggcggac ccgccgaggc gctcgccaag
     1741 ctgagcgtgt cgacccactt tgactggcgc tatgccgacg acgacctggc cgcgtcgatc
     1801 gcgcacgccc acgccctgca gcgggccagc ctcctgaccg acgccgagac cgccaagatg
     1861 gtcgacgcgc tgacggcgtt gcgagtggat gtcgctgccg gcaaggtgtt gccgtcggag
     1921 ggggatgagg acgtgcacgg tgccctcgag cgactgctga tcgaacgcat cggcccggat
     1981 gtgggcggca agctgcgtgc cgggcgttcc cgtaacgacc agatcgcgac gctgccccgc
     2041 atgtatctgc gtaggcacgc gcgcatcatc gccgcagccg tgctggacgt cgtggacgcc
     2101 ctgctggagc aggcggaccg ccactt
//
LOCUS       DASRKH010000220         3436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_18441, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000220 DASRKH010000000
VERSION     DASRKH010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18441"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..843)
                     /locus_tag="VFC06_03615"
     CDS             complement(181..843)
                     /locus_tag="VFC06_03615"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HZL81015.1"
                     /translation="MLEATGPGQVWSWDITDLRTPWRGVAFKAYSIIDIFSRKLVGCR
                     VEEREVDDLAVEMFEDAFGRHGIPDAVHADSGPAMRSGALKDLLGDLGVTQTHNRPRV
                     SNDNPFSESEFRTMKYRPNYPGTFATIDEARTYVDWYVPWYNQNHKHSGIALFSPDDV
                     HDGTWRDAWARRDATQQAYYEKHPERFRRRPHTPTPADIVGINRLKKADNETDIERLH
                     AA"
     gene            complement(1199..1441)
                     /locus_tag="VFC06_03620"
     CDS             complement(1199..1441)
                     /locus_tag="VFC06_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81016.1"
                     /translation="MTVPPEKRTGIERQRARERAAHEAEVATLTARVRELEQANEALG
                     KAIGLLHQMSEQEPAVSRPTTDPSDSSTPRTTSSDS"
     gene            1696..1845
                     /locus_tag="VFC06_03625"
                     /pseudo
     CDS             1696..1845
                     /locus_tag="VFC06_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558364.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1985..2956
                     /locus_tag="VFC06_03630"
     CDS             1985..2956
                     /locus_tag="VFC06_03630"
                     /inference="COORDINATES: protein motif:HMM:NF014898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZL81017.1"
                     /translation="MTLSDMAGRVQIYGPLEGYVPGFEAALLRSGFTSASVVNQMYLV
                     AHLSRWLESGGIDLHDLTGVHVDAFLQERKATHTALFTRRALKHLLGWLAESGVITPG
                     VVVPPPPEMSAVLGRFETYLLVERRIQARATEAYVSRVHRFLTTHAPNKALAGVSAAD
                     VTRALLAEGETRAPASVKKFRNALRAFFRFCFVTGELDHDLTGATLVPRQSLPSLLPM
                     GAKPAQIQTLLASCDRQSTAGRREYAVILLLARLGLRAGEVAKLRLEDIHWYHGEILI
                     RGKGSTEENLPLRSTWATRSRTTWLTLGPLMRCTARCSAHSTLHSGR"
     gene            3198..>3436
                     /locus_tag="VFC06_03635"
     CDS             3198..>3436
                     /locus_tag="VFC06_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81018.1"
                     /translation="MTAWADFAADYLRLRRQLGADLDWPEHLLGQFCAQLDATGCEVL
                     AVTDAIAWCSALPAGVTIAPPSRASSRMRTVRGFAT"
BASE COUNT          545 a         1118 c         1126 g          647 t
ORIGIN      
        1 cctccgagtc cggccctggc gcgctgagag cgtcaggaca ttccgttgct cgcaagtccg
       61 ggcatcatcc ccgttgttgc tgccgccggt tgacggctgc aattcatact gtcgcagatt
      121 ccggaatgat ttagggattg aatcgcgtat tttgtccgga tcagggaatc tcgcgcgatg
      181 tcaagctgcg tggagtcgtt cgatgtcggt ttcgttgtct gccttcttga gccggttgat
      241 gcccacgatg tcggctggcg ttggtgtgtg gggtcgccgc cggaaccgct ctgggtgctt
      301 ctcgtagtac gcctgctggg ttgcgtcgcg ccgagcccag gcgtctcgcc aggtgccgtc
      361 gtggacgtcg tccggggaga acaacgcgat cccggagtgc ttgtggttct ggttgtacca
      421 gggcacgtac cagtcgacgt aggtgcgcgc ctcgtcgatc gtcgcgaagg tgccagggta
      481 gttcgggcgg tacttcatgg tgcggaactc cgactcagag aacggattgt cgttgctcac
      541 gcgcggcctg ttgtgagtct gagtgacgcc gaggtcaccc aggaggtcct tcagcgctcc
      601 ggagcgcatc gcgggcccgg agtcggcgtg caccgcgtcg gggatcccgt gtcgaccgaa
      661 cgcgtcctcg aacatctcga cggcgaggtc atcgacctcg cgttcctcga cccggcagcc
      721 gacgagcttg cgggagaaga tatcgatgat cgagtacgcc ttgaacgcga caccgcgcca
      781 cggggtgcgc agatcggtga tgtcccacga ccacacctgc cccggacccg tcgcttccag
      841 caccggcgct tcccgcggcg tccggctgct gttgccacct cgcgcggcgc gaccgggcgg
      901 ccggactggt cctcgatacc ctgcgcgatt cgccaccagg aacggcggga ggcgagcatc
      961 atcccgtcgt cccatgcgga tgcgaacgag tgatcgacgg agtggccgtc cgcccagccg
     1021 gccgtgatcc gcgccgcgac caccgcccgg tcagcagcgc cgattcgcgg tggatacgcc
     1081 cggtccttgt gcggtatcgg ctcagcaacc cgctcccgcg ggttcgaccg gtagtgccac
     1141 gttgacctgg acaaaccggc gagtgcgagt gcctgccgct gcgacccgat gatcgccgtc
     1201 agctgtccga cgaggtggtt ctcggcgtcg acgaatcgga cggatcggtc gtcggccggc
     1261 tcacggcggg ctcttgctcg ctcatctgat gcaagagccc gatagctttt cccagggcct
     1321 cgttggcctg ctcaagctca cgcactcgcg cagtcagtgt cgcgacctcg gcctcgtgcg
     1381 ctgcacgctc ccgggctcgc tgtctctcga tgccggtgcg cttctcaggt gggacagtca
     1441 caagaccacc ctctcgcgga atcaaccctc ggtcgagatc accgtcgaac accgccgacc
     1501 gccaccgccg gagacgatca taagagaccc cctgcccctc gagccagatc gccttccgcc
     1561 catacagctg gagctcgtac tgatgcacga actcacggat ctgctccgtc gtgaaccccg
     1621 gactgatcga catacccgcc aactccctcg ctgatcatga atgactcaca accagcctgg
     1681 cgaagaggga gggaactcga cgagggccgc tactggtgct cggcttcgag catgtaccgg
     1741 atcgcccggg ccgcgggcca gagccgcgaa cggcgccgcc aggccaccca ccccgccaag
     1801 gtcaaacccg aactgctggc cgacggcccc tcgcaggtgt ggacctgtta tgccgatgtc
     1861 aggattatgc cgatgttgct gtctgtggct tgatggttgg cggtggccgc ggtggaaatg
     1921 tcggcataat catggctgtg tgatcggctt gtgtcctcaa tgacgaggag acaaggagaa
     1981 cgtgatgaca ctttcagata tggcaggccg ggtgcagatc tatggcccgc tggagggata
     2041 cgtcccgggg ttcgaggcgg cgttgctgcg gtcgggattc acgtcggcat cggtcgtgaa
     2101 ccagatgtat ctggtcgctc acctgagccg ttggctcgag agcggcggta ttgacctaca
     2161 cgacctgacc ggtgtccacg tggacgcgtt tcttcaggaa cgcaaagcaa cacacacagc
     2221 gttgttcact cggcgggcat tgaagcatct gctgggctgg ctcgcggaat cgggagtgat
     2281 cacgccgggc gtagtggttc ccccgccgcc ggagatgtcg gcggtcttgg ggcggttcga
     2341 gacgtatctg ctggtcgagc ggcgcataca ggcgcgcgcc acggaggcct atgtgtcgcg
     2401 ggtgcaccgg ttcctgacca cgcatgcacc gaacaaggct cttgccgggg tgagcgctgc
     2461 ggatgtcacg cgggcattgc tggcggaggg cgaaacgcga gcaccggcat cggtgaagaa
     2521 attcaggaac gccctccgcg cattcttccg gttctgtttc gtcaccggag aactcgatca
     2581 cgatctgacc ggggcgacac tcgtgccccg ccagtccctg ccgtcgctac tccccatggg
     2641 cgcaaaacca gcccaaatcc agaccctgtt ggcctcatgc gatcgtcagt caacagccgg
     2701 gcggcgtgaa tacgcggtga tcctcttgtt ggcgcggttg ggcctgcgcg cgggagaagt
     2761 cgccaagctc cggctggaag acatccactg gtaccacggt gagatcctga ttcgagggaa
     2821 aggctccacg gaggagaatc tgcccctccg gtcgacgtgg gcgacgcggt cgcggactac
     2881 ctggctcacg ctcggcccgc tgatgcgatg caccgcgagg tgttctgcac actccacgct
     2941 ccacagcggc cgttgacatc gccggcggtc tgggtcattg tgacccgtgc ctgcacccgc
     3001 gcgggccttg agccgtttgg tccccaccag ctgcgccatg gtctcgccga acagatggtc
     3061 gctgccgaag ttccctttgc cgcgatcggt caggtccttc gtcacgtcga ccccgcgacg
     3121 accgcgagct acgcccgcgt ggatgtggca cggctgcgga ctctggccca gccgtggccg
     3181 gcaggcggtg acctgttatg accgcctggg ctgatttcgc cgccgattat cttcgcctgc
     3241 gccgccagct cggcgccgat ctcgactggc ccgagcacct gctgggccag ttctgcgccc
     3301 agctggacgc tacgggatgc gaggtcctcg ccgtgactga cgcgatcgcc tggtgcagtg
     3361 cccttccggc gggcgtgacg attgcgccgc cgtcccgggc atcgagccgg atgagaacag
     3421 tccgcgggtt cgcgac
//
LOCUS       DASRKH010000221         2674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_13607, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000221 DASRKH010000000
VERSION     DASRKH010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13607"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="VFC06_03640"
     CDS             <1..978
                     /locus_tag="VFC06_03640"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZL81019.1"
                     /translation="LQATALRVRPSSLSGYVVDVQRHIIPAIGKHRLARLEPEHVEHL
                     YAELFAKGLNAGTVHHVRRTLNKALNDAVRRGRLLRNPVALANTPRYDPPDVEPLTVD
                     EARRLLVAAADEPNGAAFMLAISLGLRRGEVLGLMWDDVDLDAGRLSVRHQLERRNWK
                     HGCADPDRCDDQPSRCPQRTGGGLVLTELKTKHSRRTLPVPPPLLLALRKHRQRQREA
                     RIHAGTMWVDSGLVFTNLTGRPVSPHDHSLHWTAFLERSGVRPARLHDARHTAATFLL
                     VQGVDQRVVMDMLGWTSPTMTARYQHVVPELVEEANRRMSELLWGDGAS"
     gene            1218..1706
                     /locus_tag="VFC06_03645"
     CDS             1218..1706
                     /locus_tag="VFC06_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZL81020.1"
                     /translation="MVTQSGGAGASTYMPESEDLAEIIDFVRVLGERGVEVPEPQPAL
                     VDREGRRVEIPGPVFEALRQVVTAMSRGQGVTIAPHNTMLTTQEAADFLGISRPTLVR
                     LLTDGEIPFELRGRHRRVMLAHLLDYQDRSRTDRRAALDGMVREGEAAGLYDLHPTTV
                     TR"
     gene            1716..2264
                     /locus_tag="VFC06_03650"
     CDS             1716..2264
                     /locus_tag="VFC06_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HZL81021.1"
                     /translation="MAFPVLLDTNVLFGGYLCDTILRLAEAELFRPLWSEGVLEELSR
                     NLIIRGLRPDQVDRRIGQMRRAFPDAMVEGYEPLVAAMTCDPKDRHVLAAAVRANAEI
                     LVTFNLGDFPESSTAPYDITAVHPDDFLLDQLDLYPGLTVAALHAQSAAYAQPTRAVE
                     DVLDQLAKSGVPRFASQARRHL"
     gene            complement(2433..2517)
                     /locus_tag="VFC06_03655"
     tRNA            complement(2433..2517)
                     /locus_tag="VFC06_03655"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2481..2483),aa:Ser,seq:gga)
BASE COUNT          433 a          985 c          846 g          410 t
ORIGIN      
        1 ctccaggcca ctgcgctgcg ggtgcgaccc agctcgctgt cggggtacgt cgtcgacgtg
       61 cagcgacaca tcatcccggc gatcggcaag caccggctgg cccgcctcga gcccgagcac
      121 gtcgagcacc tgtacgccga gctcttcgcc aaggggctta acgcgggaac cgtccaccac
      181 gtgcgccgca ccctgaacaa ggccctcaac gacgccgtcc gacgcggacg cctgctccgc
      241 aaccccgtcg ccctcgccaa cacgccccgg tacgacccgc ccgacgtcga acctctcact
      301 gtcgacgagg cccggaggct gctcgtcgcc gcagccgacg agcccaacgg cgccgcgttc
      361 atgctcgcga tctccctcgg cctgcgccgc ggtgaggtcc tcggcctcat gtgggacgac
      421 gtcgacctcg acgccggccg actgtccgtg cgccaccagc tcgaacgtcg caactggaag
      481 cacggctgcg ccgaccccga ccgctgcgac gaccagccgt cacgctgccc ccagcggacc
      541 ggcggaggcc tcgtcctcac cgagctcaag accaagcact cccgccggac gctccccgtc
      601 ccgccgcccc tgctcctcgc gctccgcaag caccgccaac gccagcgcga ggcccgcatc
      661 cacgcgggaa ccatgtgggt cgactccggg ctggtgttca ccaacctgac cggacgcccc
      721 gtgtcgccgc acgaccactc gctgcactgg acggcgttcc tcgaacgctc aggcgtacgc
      781 ccggcccgcc tgcacgacgc gcgacacaca gccgcgacgt tcctgctcgt ccagggtgtc
      841 gaccagcgcg tcgtcatgga catgctcggg tggacctcgc ccacgatgac ggctcggtac
      901 cagcacgtcg tgcccgagct ggttgaggag gcgaaccggc ggatgagcga gctgctgtgg
      961 ggcgacggcg cctcgtagct caggcgagcg tgatgctcga cttggacagg gctctgcgag
     1021 tgcactcttg gcgctacttg tgccgtgact tgaacgcgcg gtctcagggg gcgcgtaggt
     1081 gctgacctgc gtgtacgcga cctggcgcgc tggacatgaa cggctacatg aacgacccgc
     1141 cgagcgcagt gcgcacgtcc tcatctggcg tacgcgtctt ctatcggtta cgcttttttc
     1201 gaataggagg cgatgtcatg gtcacgcagt caggcggtgc cggcgcgagc acgtacatgc
     1261 cggagtccga ggacctcgcg gagatcatcg acttcgtgcg tgtcctgggc gagcgcggag
     1321 tcgaggtgcc cgagccccag cccgcgctgg tcgacagaga aggccgacgg gtggagatcc
     1381 cgggtccggt gttcgaggcg ctgcgccagg tcgtgacggc catgtctcgc ggccagggcg
     1441 tgacgatcgc cccacacaac accatgctca cgacccagga ggcggccgac ttcctaggga
     1501 tctcacgtcc aacgttggtg cgcctgctga cggacggcga gatccccttc gagctgcgtg
     1561 gtcgacaccg cagggtcatg ctcgcgcacc tcctggacta ccaagatcga tcgcgcacgg
     1621 accgacgggc cgcacttgac ggcatggtcc gcgaaggcga agcggccggt ctgtacgacc
     1681 tccacccgac caccgtcacg cggtaggcgc ggagggtggc gttcccggtc ctgctcgaca
     1741 ccaatgtcct gttcggcggc tacctctgtg acaccatcct gcgcctggcg gaagccgagc
     1801 tcttccggcc gctgtggtcc gagggcgtgc tcgaagaact gagccgcaat ctcatcatcc
     1861 gggggttgcg cccagatcag gtcgatcggc gtatcggcca gatgcgccgc gctttccccg
     1921 acgcgatggt cgaaggctac gagccgctcg tcgcggcgat gacgtgcgac cccaaggacc
     1981 gtcatgtcct ggctgccgca gttcgagcca atgccgagat tctcgtcacg ttcaacctcg
     2041 gcgacttccc ggagtcgagc acggcgccgt acgacatcac ggcagtgcac cccgacgact
     2101 tcctgcttga ccagctcgac ctgtaccccg ggctcaccgt ggcggccctt cacgcccaga
     2161 gcgctgccta tgcccagccg acgcgtgcgg tggaagacgt gcttgaccag ctggccaaga
     2221 gcggcgttcc aaggttcgct tcgcaggctc gccgccacct gtagagcatc cggtgcgccg
     2281 cttccccgca gagggcgaac gccggtcacc ctcgcggtcg aatcgcctcg aactgctacc
     2341 acaactgcta ccgcgacccg ccgagcaccc tctcccgatc atgacgaagg gcccgccacc
     2401 cacatcgccg caggtcacgg gcccttcatg cgcggaggat gggggattcg aacccccgag
     2461 ggctttcacc caacacgctt tccaagcgtg cgccataggc cactaggcga atcctcccgg
     2521 ccgacagagg atacccgaga tggcaacggg ctcgtgcgcg tgcgcgcgat cgcgaccagt
     2581 ccgcgctcag tcgccgggcg gctcacgcat cgcgagccgt gtgagcgact gcgacacccc
     2641 ggcttcccgc ttgagtcgtc ggacgaaccg gtcg
//
LOCUS       DASRKH010000222         2497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_17080, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000222 DASRKH010000000
VERSION     DASRKH010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17080"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VFC06_03660"
     CDS             <1..219
                     /locus_tag="VFC06_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81022.1"
                     /translation="EQGTAVVTFKVPKSIEGDTTLTITGDTTGTVVMLDVTETKKPKS
                     NNGNGVGNAFAYGHDKEHVFAPYLPLGG"
     gene            363..536
                     /locus_tag="VFC06_03665"
     CDS             363..536
                     /locus_tag="VFC06_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1508 domain-containing protein"
                     /protein_id="HZL81023.1"
                     /translation="MYFEIYKDKAGEFRFRIKATNGNILAASEGYKAKASAVNAIDRI
                     KADAAGAETTDNS"
     gene            729..>2497
                     /locus_tag="VFC06_03670"
     CDS             729..>2497
                     /locus_tag="VFC06_03670"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865091.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HZL81024.1"
                     /translation="MLNAVGYASLDALIDEAVPESIRQDDILVLPPALTERESLAVLG
                     RFAQQNVQAVQMIGQGYYDTITPAVIRRGILSNPAWYSAYTPYQAEISQGRLEAMLNF
                     QTMVSDLTALPLANASLLDEATAVGEAVLLMQRAAKHGGAVVLDDECFPQTLEVALAQ
                     AEALGIEARVEPITEEWKPAADVTGVVIQNPGMSGVLRDLGPLIAAAHETGALVTVAC
                     DILSLTIVTPPGEIGADIAVGSAQRFGVPLFYGGPHAAFMAVREGLERQLPGRLVGVS
                     VDAAGNAGYRLTLQTREQHIRRDKATSNICTAQALLAVAASMYAVYHGPGGLRDIAME
                     VHSHAVTLYDALTAVGFHPMHEAFFDTITLSMPRGADSVVARAAAAGINIRKVDDDRV
                     AIACDQKTDLAILDKLVEAVIATKRHPVYSAPRVAIPRGMRRNSEYLTHPVFNSHRSE
                     AALTRYMRKLSDQDLALDRTMIPLGSCTMKLNAAVEMEPILWPGFANIHPFAPADQTM
                     GYRHMIEQLEDWLAEITGYAAVSVQPNAGSRGEYAGLLAIRNYHLSRGESGRNICLIP
                     DSAHGTNAASAVLVGFKVVVVKTA"
BASE COUNT          457 a          819 c          797 g          424 t
ORIGIN      
        1 gagcaaggaa cggccgtggt gacgttcaag gtccccaagt ccatcgaggg agacaccacg
       61 ctcaccatca cgggtgacac caccggcacg gtcgtgatgc tcgatgtcac ggagacgaag
      121 aagccaaagt cgaacaacgg caacggcgtc ggcaatgcct tcgcctacgg ccacgacaag
      181 gaacacgtgt tcgcgcccta cctccccttg ggaggttgac ccggagaccc ccattcgcct
      241 ctgatgctct cgacgctcgt cccctttccg ggggcgggcg tcgagagtcc ctggtcaacc
      301 accgtcttgc ctcctaggat ggtttgcacg acgaacaaca ccagacgcaa aggagctccg
      361 gagtgtactt cgagatctat aaagacaagg cgggcgaatt ccgattccgc atcaaggcca
      421 cgaacggcaa cattcttgct gctagcgagg gctacaaggc gaaggcctcg gccgtgaacg
      481 cgatcgaccg catcaaggca gatgcggctg gcgcggagac gacggacaat tcctagtgct
      541 gatgagaacg gcgccccttc ctgtgaggat gggcgccgtt ttcgtctccc tttcacggcc
      601 cggaaaacga actcgatcgg cgccgcgcgg gcagtgacct accgtcgcac cgcgcagtaa
      661 gacagaatga ccctgtgacc acctttgccg atcgccacat tggaccagag gacgagtctg
      721 tccagcggat gctcaacgca gtggggtatg cctccctcga tgcgctgata gacgaggctg
      781 tgccggagtc gatccgccaa gacgacatcc tcgtgcttcc tcccgccctg acggaacgcg
      841 agtccctcgc tgtactcggg cgctttgcgc agcagaacgt gcaagccgtg cagatgatcg
      901 gtcagggtta ctacgacacg atcaccccgg cggtcatccg acgcgggatc ctgtctaacc
      961 cggcgtggta ctcggcctat acgccgtacc aagcggagat ctcgcagggc cgcctcgagg
     1021 cgatgctcaa cttccagacg atggtgtccg acctcaccgc gctgccgctt gcgaacgcat
     1081 cgttgcttga tgaggccacc gcggtgggcg aggccgtgct gttgatgcag cgcgcggcca
     1141 agcacggcgg ggccgttgtt cttgacgacg agtgcttccc tcagacgctc gaggtcgcct
     1201 tggcacaggc cgaggccctc ggcatcgagg cgcgagtcga gcccatcacg gaagagtgga
     1261 agccggccgc cgacgtcacg ggtgtggtca tccagaaccc cggaatgtcg ggagtgctgc
     1321 gcgacctcgg gccgctgatt gccgcagccc acgaaaccgg cgcgctcgtg acggtggcgt
     1381 gcgacatcct gtcgctcacc atcgtgaccc ctcccggtga aattggcgcg gacatcgcgg
     1441 tgggttccgc ccaacgtttt ggtgtgccgc tgttttatgg cgggccgcat gccgcgttca
     1501 tggcagtgcg cgagggcctt gagcgtcaac tgcctggtcg cctggtgggc gtgtccgtcg
     1561 acgccgcggg caacgccggt taccgcctga cgctgcagac gcgcgagcag cacatcaggc
     1621 gagacaaggc cacgtcgaac atttgcaccg cgcaggcgct gctggccgtc gcggcgtcga
     1681 tgtatgcggt ctaccacggt cccggtgggc tgcgcgatat cgcgatggaa gtgcactcgc
     1741 acgccgtcac gttgtacgac gctttgaccg ccgtgggttt ccacccgatg cacgaggctt
     1801 tcttcgacac gatcacgctg agcatgccgc gcggcgcgga ctcggtggtg gcgcgcgcgg
     1861 cggctgcagg catcaacatc cgcaaggtgg acgacgacag agtcgccatc gcgtgcgacc
     1921 agaagacgga cctggcgatc ctggacaagt tggtcgaggc cgtcatcgca accaagcgtc
     1981 acccggtcta ctcggcgccc cgcgtggcca tcccccgggg catgcggcgc aactcggagt
     2041 acttgacgca cccggtgttc aactcgcacc gctcagaggc ggcgctcacg cgctacatgc
     2101 ggaagctctc ggatcaggac ctggcgcttg accgcaccat gatcccgctc gggtcctgca
     2161 ccatgaagct caacgcggcg gtggaaatgg agccgatcct gtggccggga tttgcgaaca
     2221 tccacccctt cgctcccgcc gatcagacca tgggctaccg gcacatgatc gagcagctcg
     2281 aggattggct cgcggagatc acgggctacg cggccgtttc ggtacagccc aacgccggct
     2341 cgcgcggcga gtacgccggt ttgctggcca tccgcaacta tcacctcagc cgcggagagt
     2401 cggggcgaaa catctgcctc atccccgact cggcgcacgg caccaatgcg gcgtcggccg
     2461 tcctggtcgg cttcaaggtg gtggtcgtga agacggc
//
LOCUS       DASRKH010000223         3183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_62591, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000223 DASRKH010000000
VERSION     DASRKH010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62591"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..637)
                     /locus_tag="VFC06_03675"
     CDS             complement(8..637)
                     /locus_tag="VFC06_03675"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HZL81025.1"
                     /translation="MTPLSHIDPRTMPPAEAKSAWRSAVRRVRLQRGERRMGEHADSF
                     RDQVLSLPEVQGIKCASIYASRAYEPGTLPLILALHERGVEVLMPRLGDGLVRGWGVF
                     TSADDISERAPGRPPEPSGDFLPSSRLADADLVVVPALGVDTLGTRLGQGAGWYDRAL
                     ADARPGVPLVALVFDDEFHDADMRPLPHESHDVPVTIAATPSLVARIRA"
     gene            739..1959
                     /gene="glp"
                     /locus_tag="VFC06_03680"
     CDS             739..1959
                     /gene="glp"
                     /locus_tag="VFC06_03680"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HZL81026.1"
                     /translation="MTSRSLEEHRSDVLALLAPLSPLDLTLADALGCVLAVDVVAREA
                     VPRLPIAARDGFAVRASDTRGARMRPVTLPVTHDVLPGHGPTRLMPLTAARIARGGAL
                     PYGADAVVAHPTTAAVSVFLDAEVAAGEGVVEAGGHWESGDTVVEAGTRIGAGHIASM
                     AACGFATARVRPSPRVAVVAVGSELRGHVASLHPSVAPAEPTVDASGPLLAAMLASAG
                     ARVVRTLAVPDDAPLLRQAINDAALQADLVVTLGGVSDDWHDVVGPMLKHAYDGQLRR
                     VRLMPGDQQGFGKVGDGDAAPVVLLALPGDPVDAASAFAGYGIDAIMRMRGLDVVRPT
                     AMVQQGWKAPFGFAQVVPVRRAEGTRDRVSPVGDPANPLLRDIAAADGVALVAEDTVE
                     VKKGDALPILWWQR"
     gene            1956..2546
                     /locus_tag="VFC06_03685"
     CDS             1956..2546
                     /locus_tag="VFC06_03685"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HZL81027.1"
                     /translation="MRPSEPLLERDGLRLRLLRARDEREWLSLRHRNREWLRQWEASS
                     PPGTHETPMSFRAYVRRERASWRARRTFPMVIERDGELVGRASLGRIEWGPERGGTLG
                     YWVANHVAGQGIAPAAVVLLCEYAFAQGLHRIEIAIRPENAASLAVVSKLQLREEGMR
                     ASYLFIDGAWRDHRIFAVTASEKRTGTWWTGAVEPS"
     gene            2605..>3183
                     /locus_tag="VFC06_03690"
     CDS             2605..>3183
                     /locus_tag="VFC06_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81028.1"
                     /translation="MPPAGLLAIIAVGLVLAYVLPQRIRERSDYALVRTEDRYSADMR
                     VVRATAARVAPADAASASSEIPLLVTGAARVRVATMSQAHMSRPSGPLDRAAAHAQRE
                     SLHLRRDRLGSMAQRAARARRRAFFSGVATLVTVIAWVLSATTGTPAWIAGLATGAMV
                     TVVGASVRAGAAERAALADTRAVDREVRAAATA"
BASE COUNT          466 a         1028 c         1153 g          536 t
ORIGIN      
        1 gaggatttta cgcgcgaatt cttgctacta atgacggggt cgccgcgatg gtcacgggaa
       61 cgtcgtgcga ttcgtgtggc aaaggacgca tgtcggcgtc gtggaactca tcgtcaaaca
      121 ccaaggcgac gagggggacg cccggccttg cgtccgcgag agcgcggtca taccagcctg
      181 cgccttggcc cagccgggtg cccaaggtgt cgacgcccag cgcgggcacc accaccagat
      241 cggcgtcggc gaggcgggac gacggcagga agtccccgga cggctccggt ggcctgcccg
      301 gcgcccgctc cgagatgtcg tccgcgcttg tgaagacgcc ccagccgcgc accaagccgt
      361 ctcccaggcg aggcatcagt acctcaacgc cgcgctcatg gagggcgagg atgagcggga
      421 gggtgcccgg ttcataagcg cgcgaggcgt agatcgacgc gcatttgatt ccctgcacct
      481 cgggaaggct gaggacctga tcgcggaaag agtcggcgtg ttcgcccatg cgtcgctcgc
      541 cgcgctggag tcgaacacgg cgcacggcgc tacgccacgc ggacttcgct tctgctgggg
      601 gcatggtgcg tgggtcaatg tgggacaacg gtgtcatgac gccattgtgt caaacccagg
      661 tggaccccgt gtgaccggcg aactcgtgat aatgcgccga ggttgcacgg tgttgtcgcc
      721 gcacaggaca atgccagggt gacttcccgc agcctcgaag agcatcgatc cgacgttctt
      781 gcgctgctcg cacccctttc acccctggac ctcacgcttg cggacgcctt ggggtgtgta
      841 cttgccgtcg acgtcgttgc ccgggaggcg gttccacgac tacccatcgc cgcaagagac
      901 ggcttcgccg tgagagcgtc ggacacgcgg ggagcccgga tgcgtcccgt gaccctgccg
      961 gtgactcacg acgtgcttcc ggggcacggc cccacgcgcc tgatgcccct gacggccgcc
     1021 aggatcgccc gtgggggagc cctcccgtat ggtgccgatg ccgtggtggc ccatcccacg
     1081 acggctgctg tgtcggtgtt tctcgacgcg gaggtcgcgg ccggcgaggg agtcgtggag
     1141 gcgggcggcc attgggaatc gggcgacacg gtggtcgagg cgggaaccag gatcggcgcg
     1201 ggccacatcg cgtcaatggc ggcgtgcgga ttcgccacgg cgcgggtccg tccctcgccg
     1261 cgcgtggccg tagtcgctgt gggttctgag ttgcggggcc acgtcgcaag ccttcacccc
     1321 tcggtcgcgc ccgcagagcc caccgttgac gcgtcggggc cgctgcttgc cgcgatgctc
     1381 gcgtccgcgg gggcgcgtgt ggtccgcacg cttgccgtgc cggacgatgc cccgttgctg
     1441 cgccaggcca tcaacgacgc tgctctccag gcggacctgg tggtgaccct tggaggcgtc
     1501 tccgatgact ggcatgacgt ggtggggccc atgctgaagc acgcgtacga cggtcaactc
     1561 cgccgcgtga ggctgatgcc gggcgaccag caggggttcg gcaaggtcgg cgacggcgac
     1621 gccgcgcctg tggtcttgct tgccctgccg ggagacccgg tcgacgcggc ctccgcgttc
     1681 gccggatatg gcatcgacgc gatcatgcgg atgcgcggac tcgacgtggt gcggccgacg
     1741 gccatggtcc agcaggggtg gaaggcgccc ttcggattcg ctcaggtggt gccggtgcgt
     1801 cgcgccgagg gcacgaggga tcgcgtgtca ccagtggggg atcccgcgaa tccgcttctg
     1861 cgtgacatcg cggccgccga cggtgtggcg ctcgtggcgg aagacactgt cgaggtcaag
     1921 aagggtgatg cgctccccat tctgtggtgg cagcgatgag gccaagcgag ccgcttctgg
     1981 agcgcgatgg cctgcgactg aggttgctca gggctcgcga cgagcgggag tggctctcat
     2041 tgcgccaccg caacagggaa tggttgcgtc agtgggaggc gtcgtcgcca ccaggcaccc
     2101 acgagacacc catgtccttt cgtgcgtacg tgcgtcggga acgcgcatcc tggcgcgctc
     2161 gccggacatt tcccatggtg attgagaggg acggcgaact cgtgggtcgc gcctcgctgg
     2221 gtcgcatcga atggggtccc gaacgcggcg gaacgctcgg ctactgggtc gcgaaccacg
     2281 tcgcgggtca aggtattgca cccgcggccg tggtgttgtt gtgcgagtac gcattcgcgc
     2341 agggcctgca ccgcatcgag attgcgatcc gccccgagaa tgccgcatca cttgccgtag
     2401 tgagcaagct gcagctacga gaagagggca tgcgcgcgtc gtatttgttc atcgacggcg
     2461 cgtggcgcga ccatcgcatc tttgcggtga cggcgagcga gaaacgcacc ggaacctggt
     2521 ggacgggcgc cgtagagccg tcgtgagact gacacgccgc gctaggtccc tgagacccgc
     2581 ccgacgcggc cgtaccgtcg tgtcatgcct cctgcaggac ttctcgcgat cattgccgta
     2641 ggactggtgc tcgcctacgt cctgccccag cgcatccgcg aacgaagcga ctacgcgttg
     2701 gtccgcacgg aggaccgcta ctccgccgac atgcgcgtgg tgcgggccac tgcggcgcgt
     2761 gtggctccgg cggacgccgc gagtgcctca tcggaaattc ctttgctggt gacgggtgcg
     2821 gctcgcgtgc gcgtcgccac catgagtcag gcacacatgt cgcgaccgag cgggccgctc
     2881 gaccgcgctg ccgctcacgc gcagcgcgaa agccttcact tgcgtcggga ccggctgggc
     2941 tcgatggccc agcgcgcggc tcgggcccgg cgacgcgcgt tcttctccgg cgttgccacc
     3001 ttggtcaccg tgattgcgtg ggtgctgtcg gcgaccactg ggactcctgc gtggatcgca
     3061 ggcctggcga cgggcgcgat ggtgacggtg gtgggcgctt ctgtgcgtgc aggtgctgcc
     3121 gagcgcgcgg ccctggccga cactcgcgcc gttgaccgcg aggtgcgggc agcggcaacg
     3181 gcg
//
LOCUS       DASRKH010000224         2823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_4264, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000224 DASRKH010000000
VERSION     DASRKH010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4264"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VFC06_03695"
     CDS             <1..427
                     /locus_tag="VFC06_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81029.1"
                     /translation="AADEDEQVRVYAALNRQMPPEVLESLAADKAWKVRLYALTNTSL
                     PEAAAAAMVRDRRKEVRRLALTNPKIPEVTVLRASISDRSEDVREWAWAISLTRMCHR
                     LGVDPHNFDAVEELCRQEWWEMTPESPAVVVALALSPNA"
     gene            553..1284
                     /locus_tag="VFC06_03700"
     CDS             553..1284
                     /locus_tag="VFC06_03700"
                     /inference="COORDINATES: protein motif:HMM:NF013938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZL81030.1"
                     /translation="MGRLAPTTFDRVATRSAALAARALVDANDDPEERAFLAADPDTS
                     PEALVLMADDDIGPVRNAVAANPNTPPEVCVRLSTDEEHWTRKGVAENPSTPPDVLVV
                     LSADEDWSVRRAAVRNPNVTVDLLLRLSADESEYVRDAVADHPSVPTDVLARLARDEE
                     PTVRWRIARNPNTPLVAFVPLLSDEQDVVRRAAAEASTPRICAMLGIDDDNTAAIDYL
                     REQEWWTLTPDDPAVVVALALSPNV"
     gene            1333..1908
                     /locus_tag="VFC06_03705"
     CDS             1333..1908
                     /locus_tag="VFC06_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81031.1"
                     /translation="MNTLTVAPWTESMNTMPLSDLAVLSGVSLPALSQYKSGTKPMTE
                     ATAMKIAVAAAAGPDSVILAHGLSSFPAMGAVIKREHEADPSWCVSVLAQGIQQAKSH
                     LVTDADIEAFHNKPVHVASPQWNALLHAAAVISWQQFKPGKPWWAELVPLSEPWSPID
                     HGKYVAQVWLATPILLREMNLIVGNGLTGAV"
     gene            1914..2603
                     /locus_tag="VFC06_03710"
     CDS             1914..2603
                     /locus_tag="VFC06_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HZL81032.1"
                     /translation="MQEFDKDTIESLLRELGKRLSARGVHAEVFVLGGSAMAMAYSSR
                     RVTMDIDAIFEPVEEVEREVEAMAKDMRLPKGWLNNAVRATLNGVLDDDSPWSAFEAE
                     GVKVSIASPEYILALKSMIHSRELDRDIEDAAVLCNLLGIDREDKIEAITRRYFGKDS
                     VFGAQELRLERIIDRAETLRAAGVRPPGGVSADQMKFCEPSNAGGPCGHWMKISRRYC
                     RLSAGHGGQHR"
BASE COUNT          513 a          875 c          973 g          462 t
ORIGIN      
        1 cgccgccgac gaggacgagc aggtgcgggt gtacgccgcg ctgaatcgac agatgccgcc
       61 cgaagtcttg gagtccctgg cggcggacaa ggcgtggaag gtgcgcctgt acgcgctgac
      121 caacacgtcg ctgccggagg ccgccgctgc ggcgatggtg cgtgaccggc gcaaggaggt
      181 ccggaggttg gcgctgacca acccgaagat ccccgaggtg acggtcctgc gtgcgtccat
      241 cagcgaccgt agcgaagacg tgagggagtg ggcgtgggca atttcgctga cgcggatgtg
      301 tcaccgtctc ggggtcgacc cgcacaactt cgatgcagtc gaggaactgt gccgccagga
      361 gtggtgggag atgacaccgg agagtcccgc cgtcgtggtg gcgctggccc tcagtccgaa
      421 tgcgtagccc ggggatacct tgaccacatg agtcccacct gcagcgcacc cgtcgatgga
      481 caccgcaagc cggggtctgc cgcgaagtgc cccgtgcatg gcccggccgt cggagtgcgc
      541 tctgtaatcg aggtggggcg cctcgcaccg acgacctttg accgggtggc gacccgaagt
      601 gctgcactgg ccgcccgggc gctggtcgat gcaaatgatg acccggaaga gcgggccttt
      661 ctcgcggccg acccggatac ctccccggag gctctggtgc tgatggccga cgatgacatc
      721 gggccggtga ggaacgccgt ggcggcgaac ccgaacactc caccagaggt gtgtgtgcgc
      781 ctgtctaccg atgaggagca ttggacacgc aagggtgtgg ccgagaaccc gagcactccg
      841 cctgatgtgc tggtagtgct ttccgcggac gaggactggt cggtgcgtcg tgccgccgtc
      901 aggaatccga atgtcaccgt ggacctactg cttcggttgt ctgccgatga gagcgagtac
      961 gttcgggatg ctgtggccga ccacccgagc gtccccaccg atgtgctggc gcgtctcgct
     1021 agggacgagg agcccacggt gcgctggcgc attgccagga acccgaacac tccactggtt
     1081 gcattcgtgc cattactctc agacgagcag gacgtcgtgc ggcgggccgc ggccgaggca
     1141 agcaccccgc gcatctgcgc gatgctcggc atcgacgacg acaacaccgc ggcgatcgac
     1201 tatctgcggg agcaggagtg gtggacgctg acgcctgacg atcctgccgt cgtggtggcg
     1261 ctcgcactca gtccgaacgt gtagcgcccg ctcggggtag acgtcttgcg agagatagcg
     1321 taggatataa ctatgaatac gctgaccgtt gccccgtgga ccgagagcat gaacaccatg
     1381 cccctgtccg atcttgccgt gctgtccggt gtttccctgc ccgcactgtc gcagtacaag
     1441 tccgggacca agccgatgac cgaggcgacg gccatgaaga tcgcggtggc cgctgccgcc
     1501 ggacccgact ccgtcatcct ggcgcacggc ctgtcgtctt tccccgccat gggcgccgtc
     1561 atcaagcggg aacacgaggc ggacccgtca tggtgcgtca gcgtcctcgc gcaggggatc
     1621 caacaagcca agagccacct cgtcaccgac gccgacatcg aggcgttcca caacaagccg
     1681 gttcacgttg cgtcaccgca gtggaacgcg ctgcttcacg cggccgccgt catctcgtgg
     1741 cagcagttca agcccggtaa gccgtggtgg gccgaactgg tgccgctgtc ggagccgtgg
     1801 tctcccatag atcacggcaa gtacgtggcg caggtgtggc tcgctacccc catcctcctg
     1861 cgggagatga acctcatcgt cggcaatggg ctgacggggg cggtgtagcg accatgcagg
     1921 aattcgacaa ggacaccatc gagtcgctgc tgagggaact cgggaagcgc ctgtccgccc
     1981 ggggcgtgca cgcggaggta ttcgtgctgg gcgggtccgc tatggcaatg gcgtacagca
     2041 gcaggcgcgt cacgatggac atcgatgcga tcttcgagcc cgtggaggaa gtggagcggg
     2101 aagtcgaggc gatggccaag gacatgaggt tgccgaaagg gtggctcaac aatgccgtcc
     2161 gggcgacgct gaatggagtg ctggacgacg actcaccgtg gagcgccttc gaggcggaag
     2221 gcgtgaaggt gagcatcgcg tcgcccgagt acatcctggc gctcaagtcg atgattcaca
     2281 gcagggaact cgaccgcgac atcgaagacg ccgccgtgct gtgcaacctg ctgggcatcg
     2341 accgcgagga caagatcgaa gcgatcacga gacggtactt cggcaaagac tcggttttcg
     2401 gcgcgcagga gttgcgactc gaacgcatca ttgaccgggc cgagacgcta cgtgccgcag
     2461 gggtgcgccc gccgggaggg gtgagcgccg accagatgaa gttctgtgag ccgtctaatg
     2521 ctggcggccc ttgtgggcac tggatgaaga tctctcgtcg ttactgccgg ctgtctgccg
     2581 ggcacggggg gcagcaccgt tgagtgcggc gacgttcgtc gcggtagtgg cgactgtcct
     2641 gtgcccaaca cgtagcccgc ggataccctg aacgcatgag ccgcccccag tgcactgcac
     2701 ctgttgaagg tcaccgcaag cctgggtccg ccgccaagtg tccagtgcac ggccagtcca
     2761 ccgagacgcc gcgagccaag gccccgtcaa tccgactcgt gatcgaagac aggacgcatg
     2821 acg
//
LOCUS       DASRKH010000225         2077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_16454, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000225 DASRKH010000000
VERSION     DASRKH010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16454"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..>2077
                     /gene="yicI"
                     /locus_tag="VFC06_03715"
     CDS             21..>2077
                     /gene="yicI"
                     /locus_tag="VFC06_03715"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HZL81033.1"
                     /translation="MLRGVQIVGMRGDGDSLTAFVATGRIRDRGDTLNRPLLTVTLTA
                     AGDGVIRVRLAHHLGGVPARPRFALDDAGAGAVVDGRTLHADRLSATFGDDDGDWLLT
                     FREGDRVLTSSIPRSIAHVSGPDGAFVHEQLTLDVGEYVYGLGERFGPFAKNGQSVDI
                     WNEDGGTCSELAYKNIPLYVSSRGYGVFVDSPDRVSFEVASEVVSRVQFSVPGEVLEY
                     LVIAGPTPADVLDRYTALTGRPPAVPDWSYGTWLSTSFTTNYDAETVLSFVDGMAERG
                     LPLSVFHFDCFWMRGFHWSDFVWDPDTFADPAGLIRALHERGLRVCVWINPYLAQRGH
                     LFEEGREAGYLVKRPDGSVFQWDQWQAGMALPDFTNPDATAWYVSKLRALLEMGVDAF
                     KTDFGERIPTDVVWHDGSDPQRMHNYYTHLYNKAVFDLLREVRGEGEAVLFARSATAG
                     GQQFPVHWGGDSESTFASMAETLRGGLSLAMSGFAYWSHDIGGFEGTPDAEVFKRWVA
                     FGMLSSHSRFHGSGSYRVPWQFDEEAVGVTRVFSRLKLSLMPYLAAAAVAAQERGTPL
                     MRPMVLAFPEDPACATIDTQYMLGPDLLVAPVFSASGDVAVHLPEGTWTHLLTHETVT
                     GPRWVRENHGPLSLPLYVRPGAVIARSSQTDRPEGYADDVVLQAFELGEAEVAVRVPE
                     RAAA"
BASE COUNT          265 a          739 c          747 g          326 t
ORIGIN      
        1 agacccggcc gggcctcgac gtgctccgcg gcgtgcagat cgtggggatg cggggcgacg
       61 gggactccct caccgccttc gtcgccaccg gacgcatccg tgaccgcggc gacaccctga
      121 accgcccgct cctcaccgtc accctcacgg ccgccggcga cggcgtcatc cgggtccggc
      181 tcgcccacca cttgggcggc gtgcccgccc gcccgcggtt cgcgctcgac gacgccggcg
      241 cgggcgccgt cgtcgacggc cgcaccctcc acgcggaccg cctgtcggcg accttcggcg
      301 acgacgacgg cgactggctg ctcacgttcc gcgagggcga ccgggtgctc acctcctcga
      361 tcccgcgctc gatcgcgcac gtcagcggcc cggacggcgc gttcgtccac gagcagctca
      421 ccctcgacgt cggcgagtac gtctacggcc tgggggagcg gttcgggccg ttcgccaaga
      481 acggccagtc ggtcgacatc tggaacgagg acggcggcac gtgctccgag ctggcgtaca
      541 agaacatccc gctgtacgtc tcctcgcggg ggtacggcgt gttcgtggac tccccggacc
      601 gggtgtcctt cgaggtggcg agcgaagtgg tgtcgcgcgt gcagttctcc gtcccgggcg
      661 aggtgctcga gtatctggtc atcgccgggc cgacgccggc ggacgtgctg gaccgctaca
      721 ccgccctgac gggtcggccc ccggccgtcc ccgactggtc ctacggcacc tggctgtcga
      781 cgtcgttcac caccaactac gacgcggaga cggtgctgtc gttcgtggac ggcatggcgg
      841 agcggggcct gccgctctcg gtgttccact tcgactgctt ctggatgcgg ggcttccact
      901 ggagcgactt cgtctgggac ccggacacgt tcgcggaccc cgcgggcctg atccgcgccc
      961 tgcacgagcg cggcctgcgc gtgtgcgtgt ggatcaaccc ctacctcgcc cagcgcggcc
     1021 acctcttcga ggagggccgt gaggccgggt acctcgtgaa gcggccggat gggtcggtgt
     1081 tccagtggga ccagtggcag gcgggcatgg cactgccgga cttcaccaac cccgacgcca
     1141 ccgcgtggta cgtctcgaag ctgcgtgccc tgctcgagat gggggtggac gccttcaaga
     1201 cggacttcgg cgagcggatc cccacggacg tggtctggca cgacggctcg gacccccagc
     1261 ggatgcacaa ctactacacc cacctgtaca acaaggcggt gttcgacctg ctccgcgagg
     1321 tgcggggcga gggtgaggcg gtgctgttcg cgcgctccgc gacggcgggc ggccagcagt
     1381 tcccggtgca ctggggcggg gacagcgagt ccacgttcgc ctccatggcc gagacgctgc
     1441 gcggcggcct gtcgttggcg atgtccggtt tcgcctactg gagccatgac atcggcggct
     1501 tcgagggcac cccggacgcc gaggtgttca agcgctgggt cgcgttcggc atgctgagct
     1561 cccacagccg gttccacggc tcggggtcct accgggtgcc gtggcagttc gacgaggagg
     1621 ccgtgggggt cactcgggtc ttctcgcggc tgaagctgtc cctgatgccc taccttgcgg
     1681 ccgcggccgt ggcggcgcag gagcggggga cgcccctgat gcggcccatg gtgctcgcgt
     1741 tccccgagga cccggcctgc gccacgatcg acacccagta catgctcggg cccgacctgc
     1801 tggttgcccc ggtgttctcc gcgtccggtg acgtggcggt ccacctcccc gaggggacct
     1861 ggacccatct gctcactcac gagacggtga ccggaccgcg gtgggtgcgg gagaaccacg
     1921 gcccgctctc actgccgctg tacgtgcggc ccggcgcggt gatcgcccgc agttcgcaga
     1981 cggaccgccc ggaggggtac gcggacgacg tcgtgctgca ggccttcgaa ctcggcgagg
     2041 ccgaggtggc cgtgcgggtg cccgagcggg cggcggc
//
LOCUS       DASRKH010000226         2307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_252, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000226 DASRKH010000000
VERSION     DASRKH010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="VFC06_03720"
     CDS             complement(<1..853)
                     /locus_tag="VFC06_03720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZL81034.1"
                     /translation="MATYGFLSSLPPTECGLATFADALAGAMTTPADTAIGVRVVDAA
                     EFDLPTTANISVVATLVNGDSASVAAAATALNAADVVVVQHEYGIYGGSDGAEVLDLM
                     AGLHVPIIAVLHTLLDQPLPHQKQVLEAVMRRADVSVVMSQAGLDRIRDIYDADASRI
                     VLVPHGVTPLTGPREPSEDAPIVLTWGIIGPGKGIEWGIRAMTHLSDMPARPTYRIVG
                     GTHPKVLAHAGEAYRKSLIASARRLGVENAVEFHGEYLDRDALSRHVRAASVVLLPYD
                     STSQVTSG"
     gene            complement(1089..1962)
                     /locus_tag="VFC06_03725"
                     /pseudo
     CDS             complement(1089..1962)
                     /locus_tag="VFC06_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(2010..>2307)
                     /locus_tag="VFC06_03730"
     CDS             complement(2010..>2307)
                     /locus_tag="VFC06_03730"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HZL81035.1"
                     /translation="SEPPLPADRVIIAGDDAIDAEAMTAAFATHGISKILCEGGPTLA
                     SWLLAANAVDDICLTRSAVAGGDDQPASPRIPMCFALTRRLEGDGFTMERWIRD"
BASE COUNT          374 a          711 c          809 g          413 t
ORIGIN      
        1 cgccggaggt gacttgagag gtggagtcgt aggggaggag cactaccgag gcggcgcgga
       61 cgtgcctgga gagggcatcg cggtccaagt actcgccgtg gaactcgacc gcgttttcga
      121 cgccgagccg ccgagccgac gcgatgaggc tcttgcggta cgcctctccg gcgtgggcga
      181 gcaccttggg gtgggtgccg ccgacgatcc ggtaggtggg cctcgccggc atgtccgata
      241 ggtgggtcat ggcgcggatg ccccactcga tgcccttgcc gggcccaatg atgccccagg
      301 tgagaacgat gggggcgtcc tcgctgggct cgcgcggccc ggtgagggga gtcaccccgt
      361 gggggacaag cacgatacgg gacgcgtcgg cgtcgtagat gtcgcgaatg cggtccaggc
      421 cagcctgcga catgaccacg gagacatcgg cgcggcgcat gactgcctca agcacttgct
      481 tctggtgtgg gaggggctgg tcgagcaggg tgtgtagcac cgcgatgatc ggcacgtgca
      541 gtccggccat gaggtccagc acctcggcgc cgtcggagcc gccgtagatg ccgtactcgt
      601 gttggactac gacaacgtcc gcggcgttga gggcggtggc ggccgcggcg acgctcgcgg
      661 aatcgccgtt gacgagagtg gccacgacgg agatgttggc ggtggtgggg aggtcgaact
      721 cggcggcgtc cacgaccctg actccgatgg cggtgtccgc gggagtggtc atggcgcccg
      781 ccagagcgtc ggcgaaggtc gccaggccgc attctgtcgg tggcagagaa ctcaagaatc
      841 cataggtggc cattcctcca gcctcgcctt gcctcatgct cgctattgcc aagtgcgcct
      901 gttcccactg tacgtctagg acgctggcga cgcctggcgg cagcgtcccg gcgctgagcg
      961 tcgattgcgg gctcggggtg agcgtggcgc ttggggtccc ctcgagggtg ttccgcagca
     1021 agtgctcacg tgcggagcaa gatggatttc gcgtggacga cgccgcgatc ccgtctaggg
     1081 cgtgtctgtt aattgtggat ccggtggtag gtgatcagcg atgccagtag gacgccgccg
     1141 aggtaggtcg tggcgtactt gtcgtagcgg gtcgcgatcc cgcgccactg tttgattctg
     1201 ttgaagcctc gctcgacggt gttgcgctgg cggtagatgt ccttgtcgaa tgccggtggc
     1261 ctgccgcctg ctgatccctt gcgtttgcgg tgttcaacct ggtcagagcg ctcggggatg
     1321 gtgtgaggga tgcgtcgttt gcgcagcgac tgtcgggtcg aggggtgcga ataggccttg
     1381 tctgccagca gccgaaacgg ccgccggtca gaggttctgt gcaggtcgag caggggcagc
     1441 aggtgcggat tgtcgccagc ttgaccggtc gagagcatca tcgtgacagg tgcgcagccg
     1501 gcatcggtca atgcgtggac cttggtcgtc aggccgccgc gcgaacgccc gatcgcatgg
     1561 tcaggcggtt cggcgggcaa attcttgtaa ctcgatccat ccctttttta ccccgcgggg
     1621 cgctccggca gcatgctgat ggaccttcgc gctggtcgag tccaccgaga gcaacacatc
     1681 cagatcatcg ggccctacgc cgctcgcctt cttcaccgcg gcgaacatct gcgcataggt
     1741 tccatcggct gaccagcggc gatgccgttc ccagaccgat tgccaggcac cgaagccctc
     1801 cggcagatca cgccacggcg aacccgtgcg gaaccgccag cagatcgctt ccaaggtcag
     1861 tcggtggtcg ttccacggac gaccccgacg accaccatct gccgggaccg cagtctccag
     1921 cacggaccac aactcatcac tgatcacacc cactcgtacc atacgatcca gcctcgacca
     1981 cccgaagccc aatcattagc agacacgccc tagtccctga tccaccgctc catcgtgaac
     2041 ccgtcgcctt cgagccggcg ggtgagcgcg aagcacatgg ggatgcgggg cgaggcgggt
     2101 tgatcgtcgc cgcccgccac cgctgaacgc gtgaggcaga tgtcgtcaac cgcgttggcg
     2161 gccaagagcc atgaggcgag agtgggtccg ccctcgcaaa ggatcttgga gatgccgtgt
     2221 gtggcgaagg cggcggtcat cgcttccgcg tcgatcgcat catccccggc aatgatcacg
     2281 cgatcggccg gtagcggggg ctccgag
//
LOCUS       DASRKH010000227         2639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_18432, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000227 DASRKH010000000
VERSION     DASRKH010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18432"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1531
                     /locus_tag="VFC06_03735"
     CDS             <1..1531
                     /locus_tag="VFC06_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZL81036.1"
                     /translation="RRAAYLLYHHRDLLERRGVLLIGPSTSFLRYIDHVLPSLGETGA
                     VSTTLAGLVRGIGPTVREPDDVAALKGRADMAGVIRAAVRERQRVPRTDQEFRIDGRP
                     VVFKRSDVREAQSRARRDGKPYNEARATFAKEMISRLSRQVADYLGPETEESDRQEIA
                     RDLREDRDIRVAINLCWLPVTPVELLRDLLSKRHLLDTCASRHSVAERDLLFRSADTP
                     LTDADVALLDEAAELLGDLPHGASPKHDRKAELRYAKEVLANVGGDGLVTAEMLADRM
                     RAPTLRRSVAERASEDRSWTYGHVVVDEAQELSPMAWRMLLRRCPSRSFTIVGDVAQT
                     SSPAGTQWWPETMDPQFQGGWNLRELTISYRIPAAVAIEAQSFARAAGLPVSEMSAAR
                     EVDDAVATIEVAHPGDVVERTASVAAEEVKLFAERGGGLVAIIAPADMVDALTAVAPS
                     GVEVLSARDAKGLEYDAVVIADPVAVGIVAQDLYVALTRPTRRLVLVHHGSLPTGLTA
                     H"
     gene            1618..>2639
                     /gene="serA"
                     /locus_tag="VFC06_03740"
     CDS             1618..>2639
                     /gene="serA"
                     /locus_tag="VFC06_03740"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HZL81037.1"
                     /translation="MAHVRALLLETLHPQATEILNAADIAVDNRAGALDEDELIEALQ
                     GVHLLGLRSKTHVTERVLREATDLIAVGAFSIGTNQIDLAAAARLGIAVFNAPFSNTR
                     SVVEMALSEMISLTRRLPEHNRQMHAGLWSKSAIGAHEIRGRTLGIVGYGNIGSQLSV
                     IAEALGMNVIFFDNAEKLALGNATKMPTLESLLNEADIVTLHVDGRSGNSGFFGPDQF
                     AAMKHGALLLNLSRGFIVDLDALKAGLESGQIGGAAVDVYPVEPKVKGDSFTSPLQNI
                     PNVILTPHIGGSTEEAQRDIGIFVATRLRDFVHNGSTSLSVNLPNLSLELQQGSHRIV
                     HLHINTP"
BASE COUNT          430 a          933 c          849 g          427 t
ORIGIN      
        1 acgccgcgcc gcctacctgc tgtaccacca ccgggacctg ctggagcggc gcggcgtgct
       61 gctcatcggc ccctcgacat cgttcctgcg gtacatcgac cacgtgttgc cctcgttggg
      121 cgagacgggt gcggtctcga ccacgctggc gggactggtg cgcggcatcg gcccgacggt
      181 gcgagagccc gacgacgtgg ccgcgctcaa gggtcgcgcc gacatggctg gcgtgattcg
      241 cgcggccgtc cgcgagcgcc aacgcgtgcc ccgcacggac caggagttcc gtatcgacgg
      301 ccgtcccgtg gtgttcaagc gctccgacgt gcgcgaggcc cagtcgcgag cgcgtcgcga
      361 cggcaagccc tacaacgagg cccgcgccac gttcgccaag gagatgatca gtcggctctc
      421 tcgccaggtg gccgactacc tgggcccgga gaccgaggag tcggatcgcc aagagatcgc
      481 ccgcgatttg cgcgaggacc gcgatattcg cgttgccatc aacctgtgct ggctgcccgt
      541 cacccccgtg gaactgctgc gcgacctgct ctccaagcgc cacttgctcg acacgtgtgc
      601 ctcgcggcat tccgtcgccg aacgtgactt gctgttcagg agcgccgaca cgcccctcac
      661 ggacgcggac gtggcactcc tggacgaggc ggccgaactg ctcggggatc tcccgcacgg
      721 ggcttccccc aagcacgacc gcaaggccga attgcgctac gccaaagagg tcttggccaa
      781 cgtcggcggc gatggcctcg tgacggctga gatgctggcc gaccgcatgc gggcccccac
      841 gctacgacgc tcagtcgccg agcgcgcgtc cgaggaccgt tcctggacct acggccacgt
      901 ggtggttgac gaggcgcaag aactcagccc gatggcgtgg cggatgctgc tgcgacgttg
      961 cccgtcgcgg tcctttacca tcgtgggaga cgtggcacag acgtcgtcgc ccgcgggcac
     1021 gcagtggtgg ccggagacga tggacccgca attccagggg ggctggaacc tgcgcgaact
     1081 cacgatcagc taccggatcc ccgctgcggt cgcgatcgag gctcagagtt ttgcgcgcgc
     1141 ggccggactg cccgtcagcg agatgagtgc cgcccgggag gtcgacgatg ccgtcgcgac
     1201 catcgaggtg gctcacccgg gcgacgtagt ggaacgcacg gcctccgtag cggccgaaga
     1261 ggtgaagttg ttcgccgagc gcgggggcgg ccttgtcgcg atcatcgcgc ccgccgacat
     1321 ggtcgacgcg ctgaccgcgg tggccccgtc aggcgtggag gtcttgtcgg cacgcgacgc
     1381 caagggtctc gagtacgacg ccgtggtcat tgccgatccg gtcgcggtgg gcatcgtcgc
     1441 ccaggacctc tacgtggctc ttacgcgccc cacgcgcagg ctcgtgctcg tgcaccacgg
     1501 ttcgcttccc acggggctta ccgcgcacta gcggtgtgcc agcggtgcgg cgccaggttt
     1561 gctggccaca ctcccgcggt ggcaccaagt tggtatgcgg gtcataagtg cgagatcatg
     1621 gcacacgtgc gcgcactcct ccttgaaaca cttcaccccc aggccaccga aatcctcaac
     1681 gccgctgaca tcgcggtaga caaccgcgca ggcgcgctcg atgaggacga actcattgaa
     1741 gccctgcagg gcgtgcacct cctcggactc cgctccaaga ctcacgtcac cgaacgcgtc
     1801 ttgagggagg ccaccgacct catcgcggtg ggcgccttct cgatcggcac caaccagatc
     1861 gacctcgccg ccgccgcccg cttgggcatc gcggtgttca acgccccgtt ctcgaacacg
     1921 cggtccgtcg tggagatggc cctgtccgag atgatctcgc tgacgcgccg ccttcccgag
     1981 cacaaccggc agatgcacgc cggcctgtgg tcgaagtcgg ccatcggcgc ccacgagatt
     2041 cgcggacgca cgctcggcat cgtcggctac ggcaacatcg gctcacagct gtcggtgatc
     2101 gcggaagccc tcggcatgaa cgtgatcttc ttcgacaatg cggagaagtt ggcgctcggc
     2161 aacgccacca agatgccgac gctggagtcg ctgctgaacg aggccgacat cgtcaccctc
     2221 cacgtcgacg gccgctccgg caactcgggc ttctttggcc ccgaccagtt cgccgcgatg
     2281 aagcacggcg cactgctcct caacctgtcg cgcggattca tcgtggatct cgacgcgctg
     2341 aaggcgggtc tcgagtcggg ccagattggc ggcgctgccg tcgacgtcta ccccgtggag
     2401 cccaaggtca agggcgactc gttcacctcg ccgctgcaaa acatcccgaa cgtgatcctc
     2461 accccgcaca tcggcggctc gaccgaagag gcacagcgcg acatcggcat ctttgtcgcc
     2521 actcggctgc gggacttcgt ccacaacggc tccacgtcgc tgtccgtcaa ccttcccaac
     2581 ctgtcgctcg aattgcagca gggctcccac cggatcgtgc acctgcacat caacacgcc
//
LOCUS       DASRKH010000228         4802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_17908, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000228 DASRKH010000000
VERSION     DASRKH010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17908"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..166
                     /locus_tag="VFC06_03745"
     CDS             <1..166
                     /locus_tag="VFC06_03745"
                     /inference="COORDINATES: protein motif:HMM:NF022819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3180 family protein"
                     /protein_id="HZL81038.1"
                     /translation="SHTPRREAAIAAGLAALGGAVMVAAGVIAERWCRIGPDDDNDEG
                     TDAVEAGVAH"
     gene            171..650
                     /locus_tag="VFC06_03750"
     CDS             171..650
                     /locus_tag="VFC06_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HZL81039.1"
                     /translation="MTWFNPDDVVWTPASRRLIPARLIVVAIAFVPPLVATVVLATLV
                     WSGFWWFTAGLVLIVAWCAWISVRLVLAHGWAEREDDLLVKRGRLWRSVTVVPYGRMQ
                     YVEVSSGPLARAFGIATVQLHTASPGTDASLNGVPAADAARLRDRLTSRGEARLAGL"
     gene            647..2239
                     /locus_tag="VFC06_03755"
     CDS             647..2239
                     /locus_tag="VFC06_03755"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HZL81040.1"
                     /translation="MSTLDRVYAAPREWTRVHRVSPLLGGWAVFAGVAGYWFYNMQPG
                     EVAGDEARQLHVSILLLAAAAVTGALIAIGFGYIGWYFHTFRITDEAIDQRKGVVFRQ
                     QRQARLDRLQAVDVVQPLMGRLFGFAKITVEVAGGAKSGIALQFLRLADAEALRNEIL
                     ALAAGYRADVAARHDGAGHPDGSERPDGAESGTRSGGLREELEQFTLAPAPGSARTAV
                     AAAEERLLVAVPVPRLLASIVLSWGTIASASMVLIFVVGSVVVAALVPEIDIVGAFLG
                     GGFVGLLSGAAGMVSVLLASLNRGVNFRLGISADGVRIAHGLLETRKQTVPPGRVQAV
                     HFKQSLLWRKRDWWNIVVNVAGYQDNQQAVSTLLPVGTREDALAALWSVLPDLGDPDP
                     EGTVSRALSGSGADGGFVTAPVSAKWVDPFQRRFRGVLATETALFIRRGWLARELIVV
                     PHERTQSLALTQGPIQRALGLANVAVHSTKGVVSPVAQHLDVADAVALLTAQARRARA
                     RRKVQTPEQWMEKVGLLDDGAE"
     gene            2259..3122
                     /locus_tag="VFC06_03760"
     CDS             2259..3122
                     /locus_tag="VFC06_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HZL81041.1"
                     /translation="MSVGVVGAGRVGASIGASLRLAGHLIVGASGVSEASVTRIDTML
                     PGVPRIDVEEVVRRSDLVFLAVPDDAIAPLVEGLAAVGAWRGGQLVAHCSGRLGVDAL
                     DAAAREGAIPLAIHPAMTFTGTSLDLDRMVGATFAVTAPAAFLPIAQALVVEMGGEPV
                     VLEDAARPLYHAALVHGANHVVTLVAQASALLLAAGVDDPGRLLGPLVHASVDGALAD
                     APGAVSTLTGPVVRGDAGTVASHVGALASRPEAAHAYRAMARATADLALSSGRIGPAD
                     YAAVIAALGDD"
     gene            3124..4005
                     /gene="panC"
                     /locus_tag="VFC06_03765"
     CDS             3124..4005
                     /gene="panC"
                     /locus_tag="VFC06_03765"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015772361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HZL81042.1"
                     /translation="MLIIRTAAELAEMAPARKGKKQGRRAVVMTMGALHEGHLALVRE
                     ARAAADQVVVTVFVNPLQFDDAADLERYPRDLDADAALLEPLGVDVIYAPSVEDVYPG
                     GDPMVTVSAGAINDVFEGSRRPGHFDGVLTVVLKLIQLTKPDVAYFGQKDAQQVIAIR
                     AMCRDLNVPVEIAVVPTVRAEDGLALSSRNAHLSEAERADALVLSRAVAEAQQVLDEG
                     GPLSLALTVARAAFAQVPGVVPDYIGAMDPASAQPVPDDYRGDVVIAVAAQVGGARLI
                     DNVMTRVGPREVVAPAS"
     gene            4021..4524
                     /gene="panD"
                     /locus_tag="VFC06_03770"
     CDS             4021..4524
                     /gene="panD"
                     /locus_tag="VFC06_03770"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HZL81043.1"
                     /translation="MTFKTLQRPMMISKIHRATVTQADLHYVGSITIDATLMEAADLM
                     PGQQVDVVDVTNGSRLTTYAIPGEPGTGVLCINGAAAHLVHEGDLVIIIAYGLIGDEE
                     AHQYEPHVVFVDAANHVMDVCDEPGKVPGDSGLEPSGLPISHFRSGGWKAYQSGGILT
                     SPTPDVE"
BASE COUNT          659 a         1622 c         1704 g          817 t
ORIGIN      
        1 gtcgcacacg ccccgcaggg aggccgcgat cgcggcgggc ctggccgcgc tcggtggggc
       61 cgtgatggtg gcagcgggcg tgatcgccga gcggtggtgt cgcatcggcc cggacgacga
      121 caatgacgag ggtaccgatg cggtcgaggc cggggtggca cactagccac atgacgtggt
      181 ttaaccctga cgacgtggtg tggacacccg catcgcgacg actcatccct gcccgcctga
      241 tcgtggtcgc tatcgcgttc gtgcctccgc tggtggcgac cgtcgttctc gccacgctcg
      301 tgtggagcgg cttctggtgg ttcaccgccg gtctggtgct gatcgtcgcg tggtgcgcgt
      361 ggatcagcgt gcggctcgtg ctggcgcacg gctgggcgga acgcgaggac gacctgctgg
      421 tcaagcgcgg gcgcctgtgg cgttccgtca cggtcgtgcc gtatggccgc atgcagtacg
      481 tcgaggtgtc ctccggcccg cttgcaaggg ccttcgggat tgcgaccgtg cagttgcaca
      541 ccgcctcccc gggtaccgac gcttcgctga acggcgttcc cgcagccgac gctgcgcgac
      601 tgagggaccg cctcacgtcg aggggcgagg cccggctggc ggggctgtga gcaccctgga
      661 tcgcgtctat gcggcaccac gcgagtggac gcgcgttcac cgcgtctctc cgcttctggg
      721 tggctgggcg gtcttcgcgg gtgtggccgg ctactggttc tacaacatgc agccaggcga
      781 ggtggcgggc gacgaggccc ggcagttaca cgtctcgatc ctgctgcttg cggcggccgc
      841 ggtgactggc gcgctgatcg cgatcggttt cggctacatc ggctggtact ttcacacgtt
      901 ccgcatcacc gatgaggcga tcgatcagcg caagggcgtg gtgtttcggc agcagcgcca
      961 ggcgcggctc gaccggctac aggccgtcga cgtggtgcag cccctcatgg gtcgcctctt
     1021 tggctttgcc aagatcacgg tcgaggtcgc cggcggcgcg aaatccggga tcgccctgca
     1081 gttcttgcgc cttgccgatg ccgaggcgtt gcgcaacgag attctcgcct tggcggccgg
     1141 ataccgcgcc gacgtggccg cgcgtcacga cggcgctggg catcccgacg gctctgagcg
     1201 tcccgacggc gcagagtccg gcacgcggtc agggggactg cgcgaggagc tcgaacagtt
     1261 cacgctggcg ccagcgcccg gttcggcgcg caccgccgtg gccgccgccg aggaacgcct
     1321 cctcgtcgcc gttccggtgc cgcggttgct cgcctcgatc gtgttgtctt ggggaaccat
     1381 tgcgtcggcc tccatggtcc tcatcttcgt cgtcggctcc gtggtggtgg ccgccttggt
     1441 ccccgagatc gacatcgtgg gagcgttcct gggaggcggc ttcgtgggtc tactttccgg
     1501 cgcggccgga atggtgtcgg tcctcctcgc gtcgctcaac cggggcgtga actttcgcct
     1561 cgggatttct gccgatggcg tccgcatcgc gcacggtctg cttgaaacgc gcaagcagac
     1621 tgtcccgccg ggccgggtgc aggccgtgca cttcaagcag tcgctgctgt ggcgcaagcg
     1681 tgactggtgg aacatcgtcg tcaacgtggc ggggtatcaa gacaatcagc aggccgtctc
     1741 gacgctcctg cccgtgggta cccgcgagga cgcgcttgcc gcactgtgga gcgtcctgcc
     1801 cgacttgggc gaccccgatc ctgagggcac cgtgtcgcgc gcgctgtcgg gcagcggcgc
     1861 cgacggtggc ttcgtcaccg cgcccgtgtc cgccaagtgg gtggacccgt ttcagcgtcg
     1921 cttccgcggc gtgctcgcca cggagacggc gttgttcatc cgtcgtggct ggctcgcgag
     1981 ggaattgatt gttgtcccac acgagcgcac ccaatcgctc gcactcacgc aggggcccat
     2041 ccaacgcgcg ctcggcctcg ccaacgtcgc ggtccactcc accaagggag tggtgtcccc
     2101 ggtggcgcag cacctggatg tcgccgacgc ggtggccctg ctcaccgcgc aggcgcgcag
     2161 ggctcgcgcc aggcgcaagg tacagactcc ggaacagtgg atggaaaagg tggggctcct
     2221 cgatgacggc gccgaatagc gcatcgaagc ccggacgcat gagcgtcggg gtcgtgggcg
     2281 ccggccgagt cggcgcctcc atcggcgcat cgcttcgcct cgccgggcac ctgatcgtcg
     2341 gcgcctccgg cgtgtccgag gcctcggtga cgcgcatcga cacgatgctt cccggcgtgc
     2401 cccgcatcga cgtggaggag gtggtgcgcc gctccgacct cgtgttcctg gccgttcccg
     2461 acgacgcgat cgccccactc gtggaaggac tcgctgcggt gggggcgtgg cgcggcggtc
     2521 aactcgtggc gcactgttcg ggccgcttgg gcgtcgacgc acttgacgcc gccgcccggg
     2581 agggtgccat ccccttggcg attcacccgg ccatgacctt cacggggacg tctctcgacc
     2641 tcgatcgcat ggtcggagcg acctttgcgg tgaccgcacc cgccgcgttc ctgccgatcg
     2701 cgcaggcgct cgtggtggag atgggcggcg agccggtggt gctcgaagac gcggcgcgcc
     2761 cgctctatca cgcggcactc gtgcacggcg caaatcatgt ggtgaccctg gtggcgcagg
     2821 cctccgctct actcctcgcc gcgggcgtcg acgatcccgg gaggctgctc ggaccgctgg
     2881 tgcacgcctc ggtcgatggt gcgctcgcgg atgcccccgg cgctgtctcg accctcacgg
     2941 gtccggtggt gaggggggac gctggtaccg tcgcgtccca cgtgggggcg ctcgcatcgc
     3001 gacccgaggc ggctcacgcg tatcgtgcga tggcgcgggc aaccgccgac ctcgccctct
     3061 cgtccgggcg catcggcccc gcagattacg cggcggtcat cgccgcctta ggagacgact
     3121 gacatgctca tcattcgcac cgcggcggaa ctggccgaga tggcgccagc acgcaagggc
     3181 aagaagcagg gccggcgggc cgtcgtcatg acgatgggcg cgctgcacga aggccacctc
     3241 gcgcttgtgc gcgaggcgcg cgcggccgcc gaccaggtgg ttgtcacggt gttcgtcaac
     3301 cccctccagt tcgacgacgc cgcggacctc gagcgttacc cccgcgacct cgacgcggat
     3361 gccgccctgc tcgagccgct cggcgtggac gtcatctacg cgccgtccgt cgaggacgtg
     3421 tatccgggag gcgatccgat ggtgacggtg tcggcaggcg ccatcaacga cgtctttgag
     3481 ggctcacgcc gcccgggcca cttcgacggc gtactgaccg tggtgctcaa gttgattcag
     3541 ctcaccaagc ccgacgtggc gtacttcggt cagaaggacg cgcagcaggt gatcgccatt
     3601 cgcgcgatgt gtcgcgacct caacgtgccg gtggagatag cggtggtgcc cacggtgcgc
     3661 gctgaggacg gcctggcgct gtcgagccgc aacgcgcacc tctccgaggc ggagcgtgcg
     3721 gacgcgctgg tgctgtcccg agcggtggcc gaggcccagc aggtgcttga cgagggtggg
     3781 ccgctgtcac tcgcactgac ggtggcgcgg gccgcgttcg cccaggtccc gggggtggtg
     3841 cccgactaca tcggcgcgat ggatcccgcg tcggcccaac ccgtcccgga cgactaccgt
     3901 ggagacgtgg tgatcgcggt cgcagcccaa gtgggcggcg cgcgtctcat cgacaatgtc
     3961 atgacgcgcg ttggcccccg cgaggtcgtg gcccccgcgt cgtagacgga gaggcacagc
     4021 atgacgttca agacgttgca acggcccatg atgatttcga agattcatcg cgccaccgtg
     4081 acccaggcgg acctccatta cgtgggctcc atcacgatcg acgccacgct gatggaggcc
     4141 gcagacctga tgccgggtca acaggtggac gttgtcgacg tgacgaacgg ctcgcgcctg
     4201 accacgtatg ccatcccggg cgagccgggc accggtgtcc tctgcatcaa cggcgcggcg
     4261 gcgcatctgg tgcacgaagg cgatctggtc atcatcatcg cgtatgggct cattggcgac
     4321 gaagaggccc accagtacga gccgcacgtc gtgttcgtgg atgccgcgaa ccacgtcatg
     4381 gacgtctgcg acgagccggg caaggtcccg ggcgactccg ggctcgagcc ctccggcctg
     4441 cccatttccc actttcggtc cggcggctgg aaggcatacc agtccggcgg gattctcacg
     4501 agccctactc cggacgtcga gtagaaggga ggcatacccg gcgggcctag gctgtggcca
     4561 tgacgcgctt cgtgcaaaga ctgtccgccc caacgccgtg gtggtccacc ccggcgtcgg
     4621 ggcggacagc ttgagcacga agcgcgtcat ggccacagcc taggcccgcc gagtgtgccg
     4681 ctggctctac tcgacgtctg gcgccggggt cgtcggaacc cgtcccgatg tgtaggcctt
     4741 ccagccgccg gcccggaaat cagcgatccg taggccggag ggctcgagcc cggagctgtc
     4801 tc
//
LOCUS       DASRKH010000229         2321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_13410, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000229 DASRKH010000000
VERSION     DASRKH010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13410"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VFC06_03775"
     CDS             complement(<1..240)
                     /locus_tag="VFC06_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81044.1"
                     /translation="MDFEYLLDVTPALLGKLPLVLGVVVASAAGGFTLAVLVTALRLR
                     RVPLLHPLLEGYVSFMRCTPGIIHILLVYYGLPVLL"
     gene            complement(279..1115)
                     /locus_tag="VFC06_03780"
     CDS             complement(279..1115)
                     /locus_tag="VFC06_03780"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZL81045.1"
                     /translation="MRIPTLTALATIGLLALSACSASSGDPAAPTSSTARVVTVAVEN
                     NSKPLSYTDTSGKLTGYEVELLRAVDERLPEYSFEIESSSSEDANLVGLDTGKFALVG
                     GGYYRTAAREAKYLYGQQNVGVSAILIWKRAGDTSINTIDDLATKKLAPVTPNGGIYN
                     LLVDYTNAHPNAPINIPVAENVSMAERVKGVASGQYDALVIPNNLDVASIAKELQLDV
                     TRADDPIEVRPTYVLFNKAETEVAGAVDQALIELKADGTLSKLSEQFFNEDVFTYTIT
                     KP"
     gene            complement(1135..1965)
                     /locus_tag="VFC06_03785"
     CDS             complement(1135..1965)
                     /locus_tag="VFC06_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HZL81046.1"
                     /translation="MQTFRQSRQPRPALILASTQAAGAQDSTQLKLCGIRKSFGDHAV
                     LRGIDLTIAKGDVVALIGPSGTGKTTLLRCINFLERADDGTVTIGDHTVNTRSATERE
                     ILAHRRKTAMVFQNYNLLRNKTILQNVTEGLIVAQKKPRAVAEEIAHAEIESVGLSDH
                     LHKYPYQLSGGQQQRIGIARAMALQPDVMLLDEPTSSLDPERSLEVLRVLQDVACTGM
                     TMIIATHEMSFARHVSSRVVFMEHGRVVEQGSPTQIFAHPHHERTRAFVRDMENPFPA
                     "
BASE COUNT          377 a          629 c          850 g          465 t
ORIGIN      
        1 cagcagcaca gggagtccgt agtagacgag caggatgtgg atgatgcccg gcgtgcagcg
       61 catgaaggag acgtagccct ccagcagtgg atggaggagg ggaacgcggc gcagtcgcag
      121 cgcggtcacg aggactgcga gggtgaatcc cccggctgct gacgccacaa ccacccccag
      181 cacgagagga agcttgccga gtagggccgg ggtcacgtcg agcaggtact cgaagtccat
      241 gatcgcggct cctctcgtgc tgggggtgga aggtggggtc agggcttggt gatggtgtag
      301 gtgaagacgt cctcgttgaa gaactgctcg gagagctttg acagcgtgcc gtcggccttg
      361 agttcgatga gtgcctggtc gaccgcgccg gccacctcgg tttccgcctt gttgaagagc
      421 acatacgtgg gacgcacctc gatggggtcg tcggccctgg tgacgtcgag ctggagctct
      481 ttggcgatgc tggcgacgtc caggttgttc ggtatgacga gggcgtcgta ttggcctgac
      541 gcgacgccct tgacccgctc ggccatggag acgttctccg ctacgggaat gttgatcggg
      601 gcgttcgggt gcgcgttggt gtagtcgacg agcaggttgt agatcccacc gttcggtgtc
      661 accggtgcga gcttcttggt ggccaggtca tcgatcgtgt tgatcgaggt gtccccggcg
      721 cgcttccaga tcaggatggc cgagaccccg acgttctgct ggccgtagag gtacttcgcc
      781 tcgcgggcag cggtcctgta gtagccgccg ccgaccaggg cgaacttgcc ggtgtcgagg
      841 ccgacgaggt tggcgtcctc cgatgaggag ctctcgatct cgaaggagta ctcggggagc
      901 ctctcgtcga ctgcccgcag gagttccacc tcgtacccgg tcagctttcc ggaggtgtcg
      961 gtgtaactca ggggcttgga gttgttctcg accgcgacgg tgacgactcg ggcggtcgat
     1021 gaggtgggcg cggcggggtc gccgctggaa gcggagcagg cgctgagtgc cagcaggccg
     1081 atagtggcca gtgcggtaag ggttgggatg cgcaatttgg ttgtcctttc ggggtcaggc
     1141 ggggaagggg ttctccatgt cgcggacaaa ggctctggtc cgctcgtggt ggggatgggc
     1201 aaagatctgg gtggggctgc cctgttcgac gacccggccg tgctccatga acacgacgcg
     1261 gctcgagacg tgccttgcga agctcatctc gtgggtggcg atgatcatcg tcatgccggt
     1321 acatgcgacg tcctggagta ctcgcaggac ttcgagggac cgctctggat ccagcgacga
     1381 cgtgggttcg tcgagtagca tgacatcggg ctgcagtgcc atggcgcggg cgatgccgat
     1441 ccgctgctgc tggcccccgg acagttggta cgggtacttg tggaggtggt cgctgaggcc
     1501 gacgctctcg atctccgcgt gggcgatctc ctcggcgacc gcacggggct tcttctgggc
     1561 gacgatgagg ccttcggtga cgttctgcag gattgtcttg ttgcgcagca ggttgtagtt
     1621 ctggaagacc atggctgtct tgcggcggtg cgcgaggatc tcgcgctcag tggcactgcg
     1681 ggtgttgacg gtgtggtcgc cgatcgtcac tgtgccgtcg tcggcccgtt cgaggaagtt
     1741 gatgcagcgc agcagggtcg tcttgccggt gccgctcggt ccgatgagtg ccaccacgtc
     1801 gcccttggcg atggtcaggt cgatgccacg aaggactgca tggtccccga agctcttgcg
     1861 gattccgcac aacttgagtt gtgtggaatc ctgcgcaccg gccgcttggg tcgacgccag
     1921 gataagggca gggcgcggtt gcctcgactg gcggaaagtt tgcatgctca cggctgtgct
     1981 cctcattgga gtggtattcc cgtagggagg ggttccggca gggtgtgcct ttccggaaat
     2041 gggcatggca gcgtggggcc catgcctgcg gggcgcgcag aattcgataa actaacgcgt
     2101 gctagcggat tgaattcgcg ggaaggcccg gctgcggcgt tcgatgactc cgagtaaccg
     2161 ggtcaggcga aaaggccagc agtcagagcg ggcagctcag gcggcaactg ggatgagatc
     2221 ccgtaccgga accctcaacg acaacagcac atgcacatcg acgtagacgc cgacagcacg
     2281 tggtctcggt gggttcgggt ggtgctcacg aacaggatat t
//
LOCUS       DASRKH010000230         2354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_32026, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000230 DASRKH010000000
VERSION     DASRKH010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32026"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VFC06_03790"
     CDS             <1..258
                     /locus_tag="VFC06_03790"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HZL81047.1"
                     /translation="GDDAIDAEAMTAAFATHGISKILCEGGPTLASWLLAANAVDDIC
                     LTRSAVAGGDDQPAAPRIPMCFALTRRLEGGGFTMERWIRD"
     gene            complement(255..1688)
                     /gene="gndA"
                     /locus_tag="VFC06_03795"
     CDS             complement(255..1688)
                     /gene="gndA"
                     /locus_tag="VFC06_03795"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HZL81048.1"
                     /translation="MVLAQIGVTGLAVMGRNLARNFARNGFTVAVHNRSSQKMTSLVE
                     EHGDEGSFIGSESLEDFVASLEKPRKIVIMVQAGAATDAVIDALVPLVDKGDIIVDAG
                     NAYFPDTRRREAALSEKGIHFVGSGVSGGEEGALLGPSIMPGGSAESYTSLGPILEKI
                     SAKVDGVPCCTHVGPDGAGHFVKMVHNGIEYADMQLIGEAYAMLRSGLELEPDEIAEI
                     FAEWNKGDLESFLIEITAEVLAQKDAATGKPLVDMIVDEAGQKGTGRWTVQSALDLGV
                     PVTGIAAATFARAISSQRVQREAGRKVLSASAGEYHIGNRDGFIEDVRVALYASKVVA
                     YAQGFDQIQAASDEYGWNIDRGAMARIWRGGCIIRARFLNRITEAYERNPELSSLLVD
                     PYFTDMVADGLSGWRRIVAQAALAGVPAPAFSSSLSYYDSLRAERLPAALIQGQRDFF
                     GAHTYKRIDKEGSFHTLWSGDHSEVDA"
     gene            1814..>2354
                     /locus_tag="VFC06_03800"
     CDS             1814..>2354
                     /locus_tag="VFC06_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3000 domain-containing protein"
                     /protein_id="HZL81049.1"
                     /translation="MNDTAVPAAFREALLSLQGARTRRDVRLVETPAPSRIAPFAVAL
                     NGTVLPEDLETTGRFVVLHDPAGQDAWNGTFRVIALVQAQVEAEVGQDDLWADAAWSW
                     LDEALADVPHDQFGGTVTKVVSRSFGQLAERDDQVTVELRVSWSPADSDLAPHVAAWT
                     ELLAMCAGVPPAPEGVSVLP"
BASE COUNT          409 a          786 c          737 g          422 t
ORIGIN      
        1 ggggatgatg cgatcgacgc ggaagcgatg accgccgcct tcgccacaca cggcatctcc
       61 aagatccttt gcgagggcgg acccactctc gcctcatggc tattggccgc caatgcggtt
      121 gacgacatct gcctcacgcg ttcagcggtg gcgggcggcg acgatcaacc cgctgcaccc
      181 cgcatcccca tgtgcttcgc actcacccgc cggctcgaag gcggcggatt cacgatggag
      241 cggtggatca gggactaggc gtcaacctcc gagtggtcgc cggaccacag cgtgtggaag
      301 gatccctctt tgtcgattcg cttgtaggtg tgagcgccaa agaagtcgcg ctggccctgg
      361 ataagcgccg caggcaggcg ctccgcccgc aaggagtcgt agtacgacag ggacgacgag
      421 aacgccggtg cgggcacgcc tgccagcgcc gcttgcgcga caatgcggcg ccaaccggag
      481 agtccgtcgg cgaccatgtc ggtgaaatac ggatccacca gcagactgga aagctcgggg
      541 tttcgctcat acgcctcggt gatgcggttc aggaaccttg ccctgataat gcaccccccg
      601 cgccagatgc gcgccatcgc gccgcggtcg atgttccagc cgtattcgtc ggaagccgcc
      661 tgaatctggt cgaagccctg ggcgtacgcc accacctttg acgcgtagag cgcgacgcga
      721 acgtcttcga tgaagccatc gcgattgccg atgtggtact cgccggctga tgcggacagc
      781 accttgcgtc cggcctcgcg ctgcacccgc tgcgaagaga tggcacgcgc aaacgtcgcc
      841 gcggcgatgc cggtcacggg gacgcccaga tcaagggcac tctggacggt ccagcggccg
      901 gtgcccttct gcccagcctc gtccacgatc atgtccacga gcggcttgcc ggtcgcggcg
      961 tccttctgcg cgagtacctc ggccgtgatc tcgatgagga aactctcgag gtcgcccttg
     1021 ttccactctg cgaagatctc cgcgatctcg tcgggctcca gttcgagtcc cgagcgcaac
     1081 atcgcgtagg cctcgccaat cagctgcatg tccgcatact cgatgccgtt gtgaaccatc
     1141 ttcacaaagt ggccagcgcc atccgggccc acgtgggtgc aacacgggac gccgtccacc
     1201 ttggcggaaa tcttctcaag gatcggtccg agcgacgtgt acgactctgc agatccaccg
     1261 ggcatgatcg acggtccgag cagtgcgccc tcttcgcctc cggaaacgcc cgagccgacg
     1321 aagtggatgc ccttctcgct cagggctgcc tcgcgtcggc gagtgtctgg gaagtaggca
     1381 ttgccggcat cgacgatgat gtcgcccttg tccacgagtg gcacgagcgc gtcaatgacg
     1441 gcatccgtcg ccgcgcccgc ctgcaccatg atgacaatct tgcggggctt ctcaagtgag
     1501 gccacaaagt cttcgagaga ctccgagcca atgaaggagc cttcgtctcc gtgctcttcc
     1561 acgagggacg tcatcttctg ggaactgcgg ttgtgcacgg ccactgtgaa gccgttgcgg
     1621 gcaaagtttc tcgcgaggtt ccgacccatc actgcaagtc cggtcacgcc aatctgggca
     1681 agcaccatgt cacttccttg tccgtccgag gtacgccaac ccaccctaga cgggaatgcc
     1741 tgccgcgggg tacccgcgtc cccgagggcg tggcgtaccc ggccgcggga tgcccgcgcc
     1801 ataggctcaa gccatgaatg acacggccgt tccggccgcg ttccgtgagg cgttgttgtc
     1861 cttgcaagga gcgcgtacgc gtcgcgacgt gcggttggtc gaaactcccg cgccgtcgcg
     1921 catcgcgccg ttcgcggttg ccctcaacgg caccgtgttg ccagaggacc ttgagaccac
     1981 cggacgcttc gtcgtgcttc atgatcccgc cggtcaagac gcctggaatg gcactttccg
     2041 ggtgatcgcg ctcgttcaag cccaggtgga ggcggaggtg ggccaagacg acctgtgggc
     2101 agatgccgcg tggtcatggc tcgacgaagc cctcgcagac gtcccccacg accaattcgg
     2161 cggaacggtc accaaggtag tgtcacgctc gttcggacag ttggccgaac gggacgacca
     2221 agtcaccgtc gagcttcgcg tctcgtggag tcccgccgat tcagaccttg ccccgcacgt
     2281 cgccgcgtgg accgaactgc tggccatgtg cgcaggcgtt ccccccgcgc cggagggggt
     2341 ttcagtactt ccag
//
LOCUS       DASRKH010000231         2683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_37206, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000231 DASRKH010000000
VERSION     DASRKH010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37206"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..158
                     /locus_tag="VFC06_03805"
     CDS             <1..158
                     /locus_tag="VFC06_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HZL81050.1"
                     /translation="VDQINTYDVLVNEDVVFTADALEALLAGPVKGGSVKAVATETEA
                     AAEAEEN"
     gene            160..465
                     /gene="rplW"
                     /locus_tag="VFC06_03810"
     CDS             160..465
                     /gene="rplW"
                     /locus_tag="VFC06_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010532530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HZL81051.1"
                     /translation="MTALGKDPRDILIKPVVSEKSYGLMDQGKYTFEVDPRANKTEIK
                     IAVEQIFGVKVASVNTINRKGKTRRTRFGLGKRKDIKRAIVTVAGDGAIDIFSGQAS"
     gene            482..1321
                     /gene="rplB"
                     /locus_tag="VFC06_03815"
     CDS             482..1321
                     /gene="rplB"
                     /locus_tag="VFC06_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012397691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HZL81052.1"
                     /translation="MAIRKYKPTTPGRRGSSVADFVEVTRSTPEKSLVRPLTKKGGRN
                     NTGRITTRHQGGGHKRAYRLIDFRRHDKDGVPATVAHIEYDPNRTARIALLHYADGEK
                     RYIVAPDKLKQGDSIEAGADADIKPGNNLPLRNIPVGTVIHAIELRPGGGARIARSAG
                     AKVQLVAKDGPYAQLRMPSGEIRNVDARCRATIGEVGNSEQSNINWGKAGRMRWKGKR
                     PTVRGVVMNPVDHPHGGGEGKTSGGRHPVSPWGKPEGRTRSANKESDKLIVRRRRTGK
                     NKR"
     gene            1334..1612
                     /gene="rpsS"
                     /locus_tag="VFC06_03820"
     CDS             1334..1612
                     /gene="rpsS"
                     /locus_tag="VFC06_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006823225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HZL81053.1"
                     /translation="MPRSLKKGPFIDGHLQKKVDVQNEAGTKNVIKTWSRRSVITPDF
                     LGHTLAVHDGRKHVPVFVTESMVGHKLGEFAPTRTFRGHIKDDRKGRR"
     gene            1641..2006
                     /gene="rplV"
                     /locus_tag="VFC06_03825"
     CDS             1641..2006
                     /gene="rplV"
                     /locus_tag="VFC06_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HZL81054.1"
                     /translation="MEAKAQTRYLRTTAQKARRVVNLVRGKNAVEASASLKFQPQAVA
                     IDVRKLIDSAIANARQKAEAASERFDEHELVITAITVDEGPTMKRIRPRAQGRANQIL
                     KRTSHISVTVAAPVKEGAK"
     gene            2006..>2683
                     /gene="rpsC"
                     /locus_tag="VFC06_03830"
     CDS             2006..>2683
                     /gene="rpsC"
                     /locus_tag="VFC06_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HZL81055.1"
                     /translation="MGQKVNPHGYRLGITTDHRSRWFADSTKVGERYRDYVGEDVKIR
                     DMMKKDLERAGVSKVEIERTRERVRVDLHTARPGIVIGRRGAEADKLRSSLEKLTGKQ
                     VQLNILEVKNAEIDSQLVAQGIAEQLASRVAFRRAMRKGIQSALRGGAKGIRVQCSGR
                     LGGAEMSRSEFYREGRVPLHTLRANIDYGFFEAKTTFGQIGVKVWIYKGDMTEKEWAA
                     EQAKAPRA"
BASE COUNT          570 a          834 c          840 g          439 t
ORIGIN      
        1 ccgttgatca gatcaatacg tacgacgtcc tcgtgaacga agacgtcgtg ttcaccgctg
       61 acgcccttga ggcgttgctc gcgggaccgg tcaagggtgg gtcggtcaag gccgtcgcca
      121 ccgagaccga ggccgcggcc gaggcggagg agaactgata tgaccgcact aggcaaggac
      181 ccccgcgaca ttctgatcaa gcctgtggtc tctgaaaaga gttacggcct gatggaccag
      241 ggcaagtaca ccttcgaggt ggacccccgc gcgaacaaga ccgagatcaa gatcgccgtc
      301 gagcagatct ttggtgtcaa ggtcgcgtcc gtgaacacga tcaaccgcaa gggcaagacg
      361 cgtcgcacgc gtttcggcct gggcaagcgc aaggacatca agcgagcgat cgtcaccgtt
      421 gccggtgatg gcgccattga tatcttcagc ggccaggcga gttaggtaag ggacaggaac
      481 catggctatt cgcaagtaca agccgacgac cccgggccgt cgtggctcgt ctgtcgccga
      541 cttcgtcgag gtgactcgtt cgacgcctga gaagtcgctc gtgcgcccgc tcaccaagaa
      601 gggtggacgc aacaacaccg gtcgcatcac cacccgtcac cagggtggcg gacacaagcg
      661 cgcctaccgt ctgatcgact tccgtcgtca tgacaaggac ggcgtgcccg caaccgttgc
      721 tcacatcgag tacgacccca accgcacggc gcgcatcgcg ctcctgcact acgcggacgg
      781 cgagaagcgc tacatcgtcg cgcccgacaa gttgaagcag ggcgactcga tcgaggccgg
      841 cgctgacgcc gacatcaagc cgggcaacaa tctgccgctg cgcaacatcc ccgtcggtac
      901 cgtgattcac gcaatcgagc ttcgccccgg tggcggcgcc aggatcgcgc gttcggcagg
      961 agcaaaggtg cagctcgtcg ccaaggatgg tccttacgcc cagttgcgta tgccgtccgg
     1021 cgagatccgc aacgtggatg cgcgttgccg cgccacgatc ggcgaggtcg gcaactccga
     1081 acagtcgaac attaactggg gtaaggccgg ccgcatgcgc tggaagggca agcgcccgac
     1141 cgtccgtggt gtcgtcatga acccggttga ccacccgcat ggtggtggcg agggcaagac
     1201 gtccggtgga cgtcacccag taagcccgtg gggtaagcct gagggccgta cccgcagtgc
     1261 caacaaagaa tccgacaagc tcattgtgcg ccgtcgtcgc acaggcaaga acaagcgcta
     1321 aggagcgatc gacatgcctc gtagcttgaa gaagggcccc ttcatcgatg gccaccttca
     1381 gaagaaggtg gacgttcaga atgaagccgg taccaagaat gtcatcaaga cctggtcgcg
     1441 acggagtgtt atcactcctg acttcctagg tcacacgctt gccgtgcacg atggccgaaa
     1501 gcacgtcccg gtgttcgtca ccgagtcgat ggtcggtcac aagctgggcg aattcgcacc
     1561 cacacgcacg ttcaggggcc acatcaagga cgaccgcaag ggccgccgct aaggcgcccg
     1621 gcaaacagga gtagatcatc atggaagcca aggcgcagac gcggtacctc cgcaccactg
     1681 cccagaaggc acgccgcgtg gttaacctcg tgcgcggtaa gaacgcggtc gaggcatctg
     1741 cctcgctcaa gttccagccg caggcggtgg ccatcgacgt ccgcaagctc atcgattcag
     1801 cgatcgcaaa cgcccgtcag aaggccgaag ccgcaagtga gcgttttgac gagcatgagc
     1861 tcgtcatcac cgcaatcact gtggacgagg gcccgacgat gaagcgcatt cgccctcgcg
     1921 cacagggtcg agccaaccag attctcaagc gtacgagcca catcagtgtg actgtggcag
     1981 cgcccgtcaa ggaaggggcc aagtaatggg tcagaaggtc aacccgcacg gctaccgcct
     2041 gggcatcacc accgaccacc gctcgcgttg gttcgccgac tccacgaagg tcggcgagcg
     2101 ctaccgcgac tacgtaggcg aagacgtcaa gattcgcgac atgatgaaga aggaccttga
     2161 gcgcgctggc gtgtccaagg tcgagatcga gcgcactcgt gagcgcgttc gcgtcgacct
     2221 tcacactgcg cgtcctggca tcgtgatcgg tcgccgcggc gccgaagccg acaagttgcg
     2281 ctcctctctt gagaagctca ctggcaagca ggtccagctc aacatcctcg aggtgaagaa
     2341 cgctgagatc gactcccagt tggtggctca gggcatcgcc gagcagctgg cttcacgtgt
     2401 tgctttccgt cgcgccatgc gcaagggcat tcagtcggcc cttcgcggcg gcgccaaggg
     2461 tattcgcgtg cagtgctccg gccgtctcgg tggagcggaa atgtcacgct cggagttcta
     2521 tcgtgagggc cgcgtgcctt tgcacacgct ccgcgcgaac atcgactacg gattcttcga
     2581 ggccaagacg accttcggtc agattggcgt caaggtctgg atctacaagg gcgacatgac
     2641 ggagaaggag tgggcggccg agcaggccaa ggctcctcgt gct
//
LOCUS       DASRKH010000232         2739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_20980, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000232 DASRKH010000000
VERSION     DASRKH010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20980"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            465..2414
                     /locus_tag="VFC06_03835"
     CDS             465..2414
                     /locus_tag="VFC06_03835"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HZL81056.1"
                     /translation="MLSSDAVTALAGLARVATGEFTIDELLHDLCTVAARALDVDGAG
                     VMLNGREGLIFIHAAPERVVDVEALQEILQRGPSRDSMLEREAIVLADITTSERWPEF
                     AEASAAAGLRAVVAMPLLARGEAWGVLDVYRNVAWSWTEEEVLAVSLFSGIATAYLVM
                     AADRDMARVAQRDTEHSATHDELTGLPGRVLLYSLLEKALVGAARRGTAVAVFFIDLD
                     RFKDINDVLGHAAGDAVLSEVAARLTSALRGNDIVARPSGDEFVVVCEDLPRDPAKLD
                     VELHALGHRLVTVLSRPMHLLGNDLMISASIGVAATSDHETAQKLIGNADSAMYRAKQ
                     RGRGRLVVGGGDPHSGLDSRCQLQGELEHALDRDELRVYYQPIVTVDPQHSVVAVEAL
                     LRWQHPKYGLLTAGAFISLAERSGAIHAIGRWVIEEACAQLAAWQRLLGALAPHQVFV
                     NLSPREIGDTELDAALAASLDHHGISATSIGLEIIEYDFTDALLVPRLTEHHERGHPL
                     SVDDLGIGYSSLARLVDFPVQYAKIDRSFVRGLPHDQRRKALIEAITVVAHQLGLTVV
                     AEGVETQEQVIDLTEVGCDLLQGYYLGRPQPAHALTSHWTSARDRTLAGERIAAGNRG
                     EPAEDWPTKEASPEGGAGGEAAVLM"
     gene            2514..>2739
                     /locus_tag="VFC06_03840"
     CDS             2514..>2739
                     /locus_tag="VFC06_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81057.1"
                     /translation="MDWLSSDLYGAYREWMAVAEDERMYRVVADVSRAAELSAQRLGG
                     MGDRWRHTQAVAARASSASPAVAPNDRDLLV"
BASE COUNT          450 a          899 c          897 g          493 t
ORIGIN      
        1 gcagcggccc cgatccccag tgctacagga cccgccgtcc ccgctatctc agcgactcct
       61 ttgggggtga ccttcatgcg cccgccgggc gtcagttggt cctaactaat gcccgacgcc
      121 cgctggggct ccgagccacc cctttgcgtc acagacgatc tccgtcatga tcaccaccgt
      181 acccgctgct acccgggggt cgtcttattc cactctttcc gggctagggc ctaccgagcg
      241 gggcacccaa gctcggtagg cgcgccgcga cgcgcgcact cctaaggagg ccaccccgtc
      301 cccgtcttct cgcatgtttg gtgactcaac gcgacccccc ttcgccgtca cccctttggt
      361 gaattcacgg gcacggttga catcaggtag tcacacggac acgatcggta gcaggatggc
      421 tcggtgtgcc acttagggcg gcctatccgg ctatagggga cgcgatgctt tcgtcggacg
      481 cagtgaccgc cttggccggc ctggcgcggg tggcaactgg cgagttcacc atcgatgagc
      541 tgttgcatga cctgtgcaca gtcgcggcgc gcgcgttgga cgtcgacggc gccggggtaa
      601 tgcttaatgg acgtgaaggc ttgatattca ttcacgccgc cccggagcgg gtcgtcgacg
      661 tggaggcgtt gcaagagatc ctgcagcgcg gtccgtcccg agacagcatg ctcgagcgcg
      721 aggccatcgt tctggctgac atcaccacga gcgagcggtg gccggagttc gcggaagcgt
      781 cggcagcggc aggcctccgg gcggtcgtgg caatgccctt gctggcccgc ggcgaggcat
      841 ggggtgtgct cgacgtttac cgcaacgtcg cgtggtcgtg gacggaggag gaggtcctgg
      901 cggtaagctt gttcagcggc attgccactg cctacctcgt gatggccgcc gatcgcgaca
      961 tggctcgcgt ggcgcagcgg gacacggagc actcggccac ccatgatgaa ctgacggggc
     1021 ttcccggccg ggtgctgctc tacagcttgc ttgagaaggc cttggtcggc gctgcccgac
     1081 gcgggacggc ggtggccgtt ttcttcattg acttagaccg gttcaaggac atcaacgacg
     1141 tcttgggcca cgccgccggc gacgcagtgc tctccgaggt cgcggcccgg ctgacaagcg
     1201 cgcttcgtgg caatgacatc gtcgccaggc catccggcga cgagttcgtc gtggtctgcg
     1261 aagacctccc acgcgaccca gccaagctcg acgttgagct ccacgcgctg gggcatcggc
     1321 tcgttactgt tctgagccgg cccatgcacc tattggggaa cgatctcatg atctcagcca
     1381 gcatcggcgt cgccgcgacc agcgatcacg agaccgctca gaaattgatc gggaacgccg
     1441 acagcgcaat gtaccgggcg aagcagcgcg gccgcggcag gctcgtagtg ggcggcggtg
     1501 acccccactc cgggttggac tcccggtgcc agctccaagg cgagcttgag cacgctcttg
     1561 accgcgacga gctgcgcgtg tactaccagc caattgtcac cgtcgaccca caacactcag
     1621 tggtcgcagt cgaggccctg ctgcgctggc aacatcccaa gtatgggttg ctgaccgccg
     1681 gggcctttat cagcctcgcc gaacgcagcg gagcgatcca cgccatcggc aggtgggtga
     1741 tcgaagaggc ctgcgctcaa ttagcggcgt ggcagcgcct gcttggggca ctcgcgccgc
     1801 accaggtgtt tgtcaatctt tctcctcgtg agatcggtga caccgagctc gatgccgctc
     1861 tcgctgcatc actggaccac cacgggatct cagcgaccag catcggccta gagatcatcg
     1921 agtacgactt tacggatgct ctgctggtgc cgcggctcac cgagcaccac gaacgcggac
     1981 atcccctgtc cgtggacgac ttgggcatcg gctattcctc gctggcccgt ctcgtcgact
     2041 tcccggtcca gtacgccaag atcgaccgat cattcgtgcg gggcctccct cacgaccaac
     2101 gtcgcaaggc cctcatcgaa gcgatcaccg tcgtcgccca ccagctcggt ctcacagttg
     2161 ttgccgaagg tgtcgagacc caagaacagg tcatcgacct cacagaggtt ggctgcgatc
     2221 tgctgcaggg ctactacctc ggccgcccgc aaccggcgca cgccctaacc tcccattgga
     2281 cttctgcacg cgaccggaca ctggcgggcg agcgcatagc tgctggaaat cggggggagc
     2341 cggccgagga ctggccgacg aaggaggcca gcccggaggg cggcgccggc ggcgaagccg
     2401 ccgtcttgat gtaggtagag aaactcttca cagggcctct ggtcgccccg tgtcccgctg
     2461 tcgccttcca ccgcgcggag gcgttgatcg cgggagcgga cggtgagtgc gccatggatt
     2521 ggctgtcgag cgacttgtac ggcgcgtacc gtgagtggat ggcggtggcc gaggacgagc
     2581 gcatgtaccg ggtcgttgcc gacgtgtcgc gggccgcgga attgtcggcg cagcgtctgg
     2641 gcggcatggg tgatcggtgg cggcacacgc aggctgtcgc tgctcgcgcc agctcagcca
     2701 gtccagcagt ggcgccgaac gatcgtgatc tacttgtcg
//
LOCUS       DASRKH010000233         2226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_57913, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000233 DASRKH010000000
VERSION     DASRKH010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57913"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="VFC06_03845"
     CDS             complement(<1..676)
                     /locus_tag="VFC06_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81058.1"
                     /translation="MSTQTTGAPELPANARQRSSAPKVGLKALATKNIRQSGIAIAFV
                     AIIGLFSILTGGLLLSPGNLTNIVLQYSYVLILSIGMLIVIVAGHIDLSVGSLLGLAG
                     AVSAFLVIKEGMPWWTGMLAAIAVGLIAGAWQGFWVARVGIPAFIVTLAGMLIFRGLT
                     FLVLNNISLSPFPSQYQKVASGFLNGLLGGPGYDVFTLLVFALAVAGYAVSLWRGRAA
                     KIEYNQP"
     gene            complement(677..2188)
                     /gene="mmsA"
                     /locus_tag="VFC06_03850"
     CDS             complement(677..2188)
                     /gene="mmsA"
                     /locus_tag="VFC06_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HZL81059.1"
                     /translation="MRSITKKFPGVTALDDVNLTVERGEIHAICGENGAGKSTLMKVL
                     SGLHPHGTYEGSIILDGVEVQFRDLNESEEKGIVIIHQELALSPFLSIAENIFLGNEM
                     VKNGLVDWNETNHRAGVLLARVGLRENPTTPINQIGVGKQQLVEIAKALSKDVRLLIL
                     DEPTAALNDTDSDHLLDLIRQLKELGITSIIISHKLNEIAAIADNTTIIRDGHTVETL
                     DMSDPEATQSRIIRGMVGRPLDQRFPDHIPTIGEEVFRIEDWTVHHPVQHERRVVDSA
                     DLTVRAGEVVGLAGLMGSGRTEMAMSIFGRSYGREISGKVFVRGKEVNTKGVPEAIEA
                     GIAYVSEDRKVYGLNLIQDIRTNISSAGLGKLVGRLFINGNQELHVAEKYRADLNIKT
                     PSVLNLVNSLSGGNQQKVSLSKWLYTEPEVLILDEPTRGIDVGAKYEIYVIINNLVDS
                     GKGVLVISSELPELLGMCDRIYTLSAGRITGCLDADEADQESLMHLMTMEEEA"
BASE COUNT          369 a          688 c          754 g          415 t
ORIGIN      
        1 ccggctggtt gtactcgatc ttggcggcgc gcccgcgcca caaggacacc gcgtaccccg
       61 ccaccgccag cgcgaacacc agcagggtga acacgtcata gccgggaccg ccgagcaggc
      121 cgttgaggaa gccgctggcg accttctggt actgcgacgg gaagggggag agcgagatgt
      181 tgttcaggac caggaaggtg aggccgcgga agatgagcat gcccgcgagg gtgacaatga
      241 aggccgggat gccgacccgc gccacccaga agccctgcca cgcgccggcg atgaggccga
      301 ccgcgatggc cgcgagcatg ccggtccacc acggcatccc ctccttgatg accaggaacg
      361 ccgaaaccgc gccggcgagt ccgagcagcg agcccaccga caggtcgatg tggcccgcaa
      421 cgatcacgat cagcatgccg atcgagagga tcagcacata ggagtactgc aggacgatat
      481 tggtgaggtt gccggggctc aggagcaacc cgccggtgag gatcgagaag aggccgatga
      541 tggccacgaa cgcgatcgcg attccggact ggcggatgtt cttggtcgcc agcgccttca
      601 ggccgacctt cggtgcgctt gaccgttgcc gggcgtttgc gggcagttcg ggggcgcccg
      661 tcgtctgggt gctcattcag gcctcttctt ccatcgtcat gaggtgcatc agggattcct
      721 ggtccgcctc gtccgcgtcc aggcagccgg tgatcctccc ggccgagagg gtgtagatcc
      781 ggtcgcacat ccccaggagt tcggggagct cggaggagat gacgagcacc cccttgccgg
      841 agtcgaccag gttgttgatg atcacgtaga tctcgtactt ggcgccgaca tcgataccgc
      901 gcgtcggctc gtcgaggatg agcacctcgg gctcggtgta cagccacttc gagagggaca
      961 ccttctgctg gttcccaccg gagagcgagt tcaccaggtt cagtaccgag ggcgtcttga
     1021 tgttgaggtc cgcccgatac ttctcggcga cgtggagttc ctggttgccg ttgatgaaca
     1081 gccggcccac cagcttgccc agcccggcgg aggagatgtt ggtgcggatg tcctggatga
     1141 ggttgaggcc gtagaccttg cggtcctcgg agacgtaggc gatgccggcc tcgatggcct
     1201 ccggcacgcc cttggtgttg acctccttgc cccgtacgaa cactttgccg gagatctcgc
     1261 ggccatagga gcgcccgaag atgctcatgg ccatctcggt gcgcccggag cccatcaggc
     1321 ccgccaggcc cacgacctca ccggcgcgca cggtcaggtc ggcggagtcg acgacgcggc
     1381 gttcgtgctg caccgggtgg tggaccgtcc agtcctcgat acggaagacc tcctcgccga
     1441 tggtggggat gtggtccggg aagcgctggt ccagcggacg gccgaccatg ccccggatga
     1501 tgcggctttg ggtggcctcc gggtcggaca tgtcgagcgt ctcgaccgta tgcccgtcgc
     1561 ggatgatcgt ggtgttgtcc gcgatcgcgg cgatctcgtt cagcttgtgg gagatgatga
     1621 tcgacgtgat gccgagctcc ttgagctggc ggatcaggtc gagcaggtgg tccgaatcgg
     1681 tgtcgttgag ggccgcggtg ggctcgtcaa ggatgagcag ccggacgtcc ttggacagcg
     1741 ccttcgcgat ctcgaccagt tgctgcttgc cgacgccgat ctggttgatc ggggtggtcg
     1801 ggttctccct gaggcccacg cgggccagca gcacgccggc cctgtggttg gtctcgttcc
     1861 agtcgaccaa cccgttcttg accatctcgt tgccgaggaa gatgttctcg gcgatcgaga
     1921 ggaaggggct cagcgcgagc tcctggtgga tgatgacgat gcccttctcc tcgctctcgt
     1981 tgaggtcccg gaactggacc tcaacgccat cgaggatgat gctgccctca taggtgccgt
     2041 gcgggtgcaa cccggacagc accttcatca gggtggactt accggcgccg ttctccccac
     2101 agatggcgtg gatctcgcca cgttcaaccg tcaggttgac gtcgtccaga gccgtcacac
     2161 ccgggaactt cttcgtgatg ctgcgcatct cgagaatggt gtcgctcacg cgttctcctc
     2221 ttcgtg
//
LOCUS       DASRKH010000234         2102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_24493, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000234 DASRKH010000000
VERSION     DASRKH010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24493"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            392..1021
                     /locus_tag="VFC06_03855"
     CDS             392..1021
                     /locus_tag="VFC06_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81060.1"
                     /translation="MWLAPSHPGSLLFLGTAYTVIGTAVIEYPPTHVAKGTTVSQEEW
                     GIYLVGLAVVFLIGFLFSAVAITTRQWILTRFGDPDRALGRAAAALLREIIRPQTPEL
                     QHKRTVLAAIRAIHERAPRAGITDLAALEHFVASAEPRASLELARNTRALIVDHVHGN
                     LISLDRPVEQREWSGTVFGSRLIAGEATGFVFTITLLTAVVGLVRALVQ"
     gene            complement(1080..1760)
                     /locus_tag="VFC06_03860"
     CDS             complement(1080..1760)
                     /locus_tag="VFC06_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HZL81061.1"
                     /translation="MGTTDRVAARERQWDGALDRSDYSDGFVRGRDELADAARDAQWE
                     RVLGLLRDPKRRLMADHWRVGGASWFTPLHQAAWSGAPEAVVAELLELGASRALVTAD
                     GRTARDIAAAHGHDALVPLLEPESRNPANADVLAILDRRLVDLVEHRIRPCLTVGLRP
                     FPTVTLTELEWGTRVWLPVPGMYGGFSVALMCNYLDVQSWSRVVGGSGQAHVITAQGT
                     VLVDEGLI"
BASE COUNT          350 a          736 c          664 g          352 t
ORIGIN      
        1 aggtgcacgc gtggtggaac ccttgagtag cccctcgatt cacccagcca cggccagcgc
       61 cgaagttatg gccacaaccg ccggcgccga ttccgcggct cgcgctccag atggtctcga
      121 tgcgccgtgc gaatccctcg caggtccgat ggactcggat tcgaacaagt ccattcgcag
      181 cctttggagc agactacgcg acccgcgtga cacagtccgc gccgacttac tgcgcgtcct
      241 agcacgggtg ttgtcgccgt cacgaaggtt ccgtgccggt cgtgaccgca gacgactcac
      301 gcggctagga tttgacgatt cgctcatcaa ctcactcgag gcagcatggt cgtcccgcga
      361 agcagacagg actgcccgac ggtcggtgtc catgtggctt gcaccgtcgc atccagggtc
      421 cctgctgttt ctcggaaccg catacaccgt catagggacc gctgtcatcg agtatccccc
      481 cacacacgtc gcgaagggca ccaccgtctc gcaggaggaa tggggcatct accttgtggg
      541 cctggcggtc gtcttcttga tcgggttcct cttctcggcg gtcgccatca ccacacggca
      601 gtggatcctc acgcgcttcg gcgacccgga ccgcgccctg ggacgggccg cggccgctct
      661 cctacgagag atcatcaggc cgcagacgcc agaactgcag cacaagcgca ccgtccttgc
      721 cgcgattcgc gccattcacg agcgcgcccc acgtgccggg atcactgatc tggccgcgct
      781 cgagcatttc gtcgcctccg ccgagcctcg cgcctcactg gaactcgcga ggaacactcg
      841 cgcgctgatt gtcgaccacg tacacggcaa tctgatcagc ctggaccgcc ccgtcgaaca
      901 acgcgaatgg tctgggaccg tgttcggaag ccggttgatc gcgggcgaag cgaccgggtt
      961 tgtcttcaca atcacactgc tgaccgcggt cgttggcctc gtcagagcct tagtccaata
     1021 gtccgttgca tgggcttcct ggccagtcgc tcggggcaga gcaggcacag gtggtgacgc
     1081 tagatgagac cttcatcgac gaggacagtg ccctgggccg tgatgacatg ggcttggccg
     1141 gagccaccca ccacgcgact ccagctctgc acgtcgaggt agttgcacat caacgcgacc
     1201 gagaacccgc cgtacattcc gggaacgggc aaccagacgc gagtgcccca ctccagctcg
     1261 gtgagcgtca ccgtcgggaa gggtcgaagt ccgacagtga ggcatgggcg gatccgatgc
     1321 tctacgagat cgaccaagcg ccggtcgagg atggcgagga cgtccgcgtt ggcgggattg
     1381 cgcgactcgg gttcaagcag cgggaccagc gcatcgtgcc cgtgcgccgc ggcgatgtct
     1441 cgggcagtgc gcccatcggc ggttaccagt gcgcgggatg cgcctagttc cagcagctcc
     1501 gcgacaacgg cctctggcgc tcccgaccag gctgcttggt gcagcggcgt gaaccacgag
     1561 gcgcctccga cccgccagtg atcggccatc agtcttctct tggggtcccg cagcagcccc
     1621 aggacgcgtt cccactgtgc gtcgcgggca gcgtcggcaa gctcgtcgcg cccgcggacg
     1681 aacccgtccg agtagtcgct gcggtccaga gcgccgtccc actgtcgttc cctggcggct
     1741 acccgatcgg tggtgcccac gtgcctcctt ggagttgggc ccaagctctc gcccgcggca
     1801 agtctgccgt agctctcggc ggcgcgggga tgcgcttccg gcacccagtc ggcaaccccg
     1861 gggcgactgg cggcgagctg gctgagcgcc aagaccgatc acgcgcgcaa gagcctctcc
     1921 gcagctcgga ccacctccgc tgctgcccgc cgatcacggc ggcccgctcg ccgtcggtcg
     1981 cgcgcgtcac aagcaccagc gccacgcgac gcacggcatt cggctcgccg tgcggcgacg
     2041 acgacttcct tgtcgagtga gccgacgaga gtccccgagt cactgccgag cctgatcagg
     2101 ac
//
LOCUS       DASRKH010000235         2277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_31967, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000235 DASRKH010000000
VERSION     DASRKH010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31967"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..470)
                     /gene="rnpB"
                     /locus_tag="VFC06_03865"
     ncRNA           complement(102..470)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFC06_03865"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            538..1076
                     /gene="yaaA"
                     /locus_tag="VFC06_03870"
                     /pseudo
     CDS             538..1076
                     /gene="yaaA"
                     /locus_tag="VFC06_03870"
                     /inference="COORDINATES: protein motif:HMM:NF015820.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
     gene            1092..1271
                     /locus_tag="VFC06_03875"
     CDS             1092..1271
                     /locus_tag="VFC06_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81062.1"
                     /translation="MAKHTRGLLTAHLLSFETAPDSAQDVADAAAGLLGVSLVDVSLE
                     ARTKGPDELTVVVAG"
     gene            complement(1268..1609)
                     /locus_tag="VFC06_03880"
     CDS             complement(1268..1609)
                     /locus_tag="VFC06_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81063.1"
                     /translation="MAFLHAVLAIANLARNKGVLLRLMGSGTLEWPQSYQETWLRRPR
                     EWVDGKQILVVELSKMGVPSRVEPRVTLKGATHDLPNLPLYRASVDEFCKKVNEFVAE
                     RGIVLQTERLR"
     gene            complement(1692..>2277)
                     /locus_tag="VFC06_03885"
     CDS             complement(1692..>2277)
                     /locus_tag="VFC06_03885"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HZL81064.1"
                     /translation="LNAVGRIQAAKAADAVYDIGRRTWPSAAKVSRVIASPMVRTQET
                     GAAIGRRLGMHPEADDRLREIHFGAWEGLTAEEISAADPHAMHRWRFGEIPAEGGESF
                     GDVGSRVDGLLVDLAAQHAELASVGGDEERTWVAVSHAVAIKCAVGVSMGMPANRWGA
                     VWPVPASLTMLQLSISTTGQIVERHLMSLGSPTD"
BASE COUNT          368 a          811 c          721 g          377 t
ORIGIN      
        1 caatctctcg gcgcacggcg cgtgaaagtt cgggcggtgc ggacaccgtg cggacaacgc
       61 agaggtgcgt ggagcaaact acctggtaaa gcacccgagg gcggatgagt cggccgatac
      121 gccgggttct gtcgtcgtgc acggggcacg acgggcggcc atccatctac gacgtacgtt
      181 gccgcacgcc tctagcaacc tacccagatg ctcgggcgag cagccctcaa acacatcctg
      241 tttggtcttg ctccgggtgg ggtttgcctt gaccgccgct gtcaccagcg acgcggtgag
      301 ctcttacctc accttttcac ccttacctcc ggccgaaacc ggaggcggtt attttctgtg
      361 gcactttccc gcgggtcgcc ccgggtggct attaaccatc accctgctct acggagcccg
      421 gacgttcctc ggtgcgggtt tccccgcaac gcgaccgcct ggccaactca tccgcgactc
      481 aagcataggg tgtagcccgt gctgctgctc ctcccgccct cagagggcaa gacccctgtg
      541 acgtcgggag acccggtcga cctgacgaaa ctcagccatc cgatgctggc cgacgcgcgc
      601 cgacgggtgg gtgacgcact ggccaaggtg agcggccagc gcaacgccat gaaggcgctg
      661 gacgctggcc cctcgctcgc ggacgacgtc gcccgcaaca cccgactgtg ggcggagcca
      721 acgggcccgg cggcctcggt ctacacgggc gtgctgtacg aggcggcggg catggcggcg
      781 tgggacgacg cgacgatgct tcgcgcacac gagcgcatca ggatcatctc cgccctgtgg
      841 ggcgcggtct cccccgccga cctcatctcc gcctaccggc gtcgatggcg accgcgctgg
      901 gccgcatcgg gccgctggga gcgttctgga ggaagcacct cgacgttccc ctcaccgctg
      961 tggccgacgg ctccgtcgtc gtcgactgcc ggtcgagttc ctacgccgtc gcgtggacgc
     1021 cccgggaaac cccgtgggtc gccgtcaagg tgctgcgcga cctcgacggc aagcgcagcg
     1081 tcgtgagtca catggccaaa cacacgcgcg gtttgctcac ggcgcacctc ttgagcttcg
     1141 agaccgcccc cgattcagct caggacgtcg ccgatgctgc ggcggggctg ctcggggtat
     1201 cgctcgtcga cgtgtcgctc gaggcgcgga ccaaggggcc cgacgaactc accgtggtcg
     1261 tcgcgggcta gcggagccgt tccgtctgga gcacgattcc gcgctccgcc acgaactcgt
     1321 tgaccttctt gcagaactcg tcaacgctcg cccgatagag cggcaggttc ggcaggtcgt
     1381 gagtggctcc cttgagcgtc acccgcggtt caacgcgcga aggcaccccc atcttggaca
     1441 gctcgaccac caggatctgc ttgccgtcca cccattcccg aggccgacga agccaggtct
     1501 cttggtacga ctgcggccac tccaaagtcc cagagcccat cagccgaagc agcacgccct
     1561 tgttgcgagc gaggttcgcg atggcgagca ccgcgtgcag gaaggccacc gccgccgacg
     1621 tgggttcacg tccacacggt cacgggttcc cgccatgcat ccgacatcaa tgatcttctt
     1681 ttcggctgac cctagtctgt gggcgagccc agggacatga gatgcctttc cacgatctga
     1741 ccggtcgtcg agatactgag ctgaagcatc gtcagcgacg ccggaactgg ccagaccgca
     1801 ccccaacggt tggccggcat gcccatcgac acgcccacgg cgcacttgat cgcgaccgca
     1861 tgggacacgg caacccacgt gcgctcttcg tcgccgccga cgctcgccaa ctccgcatgc
     1921 tgcgccgcga ggtcgacaag gaggccgtcg actcgcgagc ccacgtcgcc gaacgactcc
     1981 ccgccctcgg cgggaatctc accgaaccgc caccgatgca tggcgtgcgg atcggccgct
     2041 gagatctcct ctgcagtgag gccctcccac gcgccaaagt ggatctctcg cagccggtcg
     2101 tccgcctcgg gatgcatccc cagacgtcgg ccgattgcgg cgcccgtctc ttgggtgcgc
     2161 accatgggcg acgcgatgac gcggctcacc ttggcggccg acggccaggt gcgacgcccg
     2221 atgtcgtaca cggcgtcggc ggccttggcg gcttgaatgc gccctaccgc gttcaag
//
LOCUS       DASRKH010000236         2220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_26568, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000236 DASRKH010000000
VERSION     DASRKH010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26568"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1952)
                     /locus_tag="VFC06_03890"
     CDS             complement(<1..1952)
                     /locus_tag="VFC06_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZL81065.1"
                     /translation="MTTTSDRSSDIAGSFLVNAGRFFTPGTISDDLRTVTREGGRDAD
                     SFYRDRWSHDKIVRSTHGVNCTGSCSWKVYVKDGVITWETQQTDYPSVGPDSPEYEPR
                     GCPRGAAFSWYTYSPTRVRYPYVRGVLLEAYRAAKLVHADPVDAWKAVVSDPETRRRY
                     QQARGKGGLVRASWDEAKEIVAAAHVHTIKDHGPDRVFGFSPIPAMSMASHAAGARFI
                     SMLGGSMLSFYDWYADLPVASPQVFGDQTDVPESADWWNASYLIMWGANVPVTRTPDA
                     HFMAEARYRGQKVVTVSPDYADNTKFADEWMSPHPGTDGALAMAMGHVVLKEFFIERQ
                     VPRFVDYVKKFSDLPFLVSLREKNGAWVPDKFVTAADLGDTSENAQFKTVLIDSATGA
                     PHVPNGSLGHRFGDEGIGKWNLDLEGVEPALSFYQATGTEVAELLLPRFDLGQEVNEG
                     GGVHHRGVPAARLETVHGKRLVTTVFDLMLAQYGVGRDGLPGEWPTGYDDPTPYTPAW
                     QETITGVPAAMAERIGREFAQNAEDSGGRSMIILGAGTNHWFHSDETYRTFLALITLT
                     GCQGVNGGGWAHYVGQEKVRPFTGWATLAFGLDWVRPPRQMIGTAFFYVNSGQWRYDG
                     MGPEALSSPLGEGTFKGLQSIDTIAQS"
     gene            complement(1949..>2220)
                     /locus_tag="VFC06_03895"
     CDS             complement(1949..>2220)
                     /locus_tag="VFC06_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81066.1"
                     /translation="STLGDSITIGTCPYASLIAEHPVICDIHTELLATVLNAAGQGVG
                     VEAMDVWVKPTLCRARLDRPDISPARSITVSPADLTEPTRERDQQ"
BASE COUNT          361 a          689 c          771 g          399 t
ORIGIN      
        1 gattgcgcga tcgtgtcgat ggattgaagg cccttgaagg tgccctcgcc aagcggggag
       61 gacagcgcct ccggtcccat gccgtcgtag cgccattggc cggagttgac gtagaagaac
      121 gcggtgccga tcatctggcg aggggggcgc acccagtcga gaccgaaggc caacgtggcc
      181 cagcccgtga acgggcggac cttctcctga cccacgtagt gtgcccagcc gccaccgttg
      241 acgccctggc agccggtgag cgtgatgagg gcgaggaacg tacggtatgt ctcgtcggag
      301 tggaaccagt ggttggtgcc cgctccgagg atgatcatcg atcggccgcc cgaatcttcg
      361 gcgttttgcg caaattcacg gccgatgcgc tcggccatgg cggcagggac gccggtgatg
      421 gtctcttgcc acgctggcgt atagggcgtc gggtcgtcgt agcctgtggg ccactcgcct
      481 ggtagaccgt cgcgacccac gccgtactgc gcgagcatca ggtcgaacac ggtggtgacc
      541 agtcgcttgc cgtggacggt ctcgagcctc gctgcgggta cgccgcggtg gtggacgccg
      601 ccgccctcat tgacctcctg gcccaggtca aaacgcggca gcagcagttc cgcgacctcg
      661 gtgcccgtgg cctggtagaa ggacagtgcg ggctccacgc cctcgaggtc gaggttccac
      721 ttgccgatgc cctcgtcgcc aaagcggtgc ccgagcgagc cgttcggtac gtgaggtgca
      781 cccgtcgccg agtcgatcag cacggtcttg aactgggcgt tctcgctggt atcgccgagg
      841 tcggcagcgg tcacgaactt atcgggaacc caggcgccgt tcttctcgcg caggctcacc
      901 aggaacggca ggtcgctgaa ctttttcacg tagtccacaa agcgcgggac ctggcgttcg
      961 atgaagaact ccttgagcac cacatggccc atcgccatag ccagcgcgcc gtcggtgccg
     1021 ggatgcgggc tcatccactc gtctgcaaac ttggtgttgt cggcatagtc gggggagacg
     1081 gtgacgacct tttgaccgcg gtagcgcgcc tccgccatga agtgcgcgtc gggcgtgcgg
     1141 gtgacgggga cgttcgcccc ccacatgatc aggtacgacg cgttccacca gtctgcggac
     1201 tcgggaacgt ctgtttggtc gccaaatacc tggggtgacg cgaccgggag gtcggcgtac
     1261 cagtcgtaga acgacaacat cgacccgccg agcatgctga tgaagcgggc acccgcggcg
     1321 tgagaggcca tggacatcgc ggggatgggg gagaagccga agaccctgtc ggggccgtgg
     1381 tccttgatgg tgtggacgtg ggcggcggcc acgatctcct tggcctcgtc ccatgaggcg
     1441 cgcacgagcc cgcccttgcc gcgcgcttgc tggtagcgtc ggcgcgtctc ggggtcgctc
     1501 acgacggcct tccacgcgtc gaccgggtcg gcgtggacca gctttgccgc cctataggcc
     1561 tcgaggagca cgccgcgcac gtacgggtag cgcacgcgcg tgggggagta ggtgtaccaa
     1621 gagaaggcgg cgccacgagg gcagccgcgc ggctcgtact cggggctgtc aggccccacc
     1681 gagggatagt cggtctgctg cgtctcccac gtgatcacgc cgtctttgac gtagaccttc
     1741 caggagcacg atccggtgca gttcaccccg tgtgtgctgc gcacgatctt gtcgtggctc
     1801 cagcggtcgc ggtagaacga gtctgcgtct cgcccgccct cgcgggtgac ggtgcgcagg
     1861 tcatccgaaa tggtgcctgg cgtgaagaac cggcctgcgt tcaccaagaa actgcctgca
     1921 atatcgctgg agcggtcgga cgtcgtcgtc attgttgatc cctttcacgg gtcggttccg
     1981 tcaagtctgc tggagaaacg gtaatgctgc gagcggggga gatgtccggg cggtcaagcc
     2041 tggccctgca cagcgtcggc ttgacccaca cgtccatcgc ctcgacgcct acgccctggc
     2101 ccgccgcgtt gaggactgta gcgaggagtt cggtgtgaat gtcgcagatg acgggatgct
     2161 cggcgatcag cgacgcgtag gggcacgtgc cgatcgtgat ggaatcaccc agggtcgatt
//
LOCUS       DASRKH010000237         2773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_15361, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000237 DASRKH010000000
VERSION     DASRKH010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15361"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="VFC06_03900"
     CDS             <1..865
                     /locus_tag="VFC06_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81067.1"
                     /translation="INGVDTDSEKAAKKATAASGRHRDYEATARDDLATLSAAVESAK
                     TEAAESSEGRMLDGLRESKRTLQGKVRQADTLSGRLAVLRKSIRDTTESMRGRQDAIK
                     DDVERVDALDQVVTACRPQGIPSMLLDGVLAPIEVEATRILNEVPGGEGMALRFEQAR
                     ALKSRVGAREVLDIVVTLPSGVERPIESLSAGESVRVSLSLVFAMISVLGARRGGDAL
                     DTVFLDEPLGPLDQEAVPAFVQVLRSAVTAGSLSSVMVVTHDQRVIDALPQRIWVERS
                     EVGDSVATITT"
     gene            897..1436
                     /locus_tag="VFC06_03905"
     CDS             897..1436
                     /locus_tag="VFC06_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81068.1"
                     /translation="MRDLRALLSQTRREISKRQLDEGNQDPELLAQAALQSLFTETSV
                     GAPKVVAPGVLALGGQVQEHAPGSTRHKCQLFTVLPDGEGSTEMWAWDDSIETFMYSE
                     TAKVATRRRSVALHAVTEGAIVVRHSMTHDGFRHNRTAVAAWRVSSVDDDGNARIEQL
                     SGWVPSTLPLSHDTSGDYR"
     gene            1436..>2773
                     /locus_tag="VFC06_03910"
     CDS             1436..>2773
                     /locus_tag="VFC06_03910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HZL81069.1"
                     /translation="MSNKPRLVEINTFFDEMPESNIEHETKGGRLATLLVGAVAFLAI
                     VLIVPTIIVLPMAFAIDAVMQEARGAWHAVPATVPEVPSTLPGFTYVYDSAGNVVAQF
                     YAENRQPVTFDEVSPLLVDAIVSVEDRRFWDHNGVDGNSVVRAAYANYIAGGAVQGAS
                     TLTQQLVENLKLLSAGSAEERDLARAQTLGGKVVEAKTALAMEAQMSKNEILEAYMNV
                     VYFGAGSYGVAAAADRFFGVDVADLSVAQAATIAGMVREPSGLDPFRFPERAQSRRDT
                     VLLAMRETGAITLWEYVTATNSPLDTSGGNQQPNGCTTSAYPFYCSIVREQLLVDGRL
                     GRTQPQRDNAIAQGHLAVYTALDPAVMASIDASIAAAFDDSNRVATSVAVVEPGTGLV
                     KGFGQNRQWGDGEGETEIPYVLSGFQPGSAFKAVIMAAALENGFNAHTAMSTRS"
BASE COUNT          491 a          928 c          887 g          467 t
ORIGIN      
        1 catcaacgga gtcgacaccg acagcgagaa ggccgcaaag aaggcgaccg ccgcatccgg
       61 gcgtcaccgt gactacgagg ccaccgccag agacgacctc gccaccctgt ccgccgcagt
      121 ggagtccgca aagaccgaag ccgcggagag cagcgagggt cggatgcttg acggtctgcg
      181 cgagtccaag cgcaccctgc agggcaaggt ccgccaggcg gacacgctca gcgggcgact
      241 ggctgtactg cgcaagtcca tccgcgacac cacggagtcc atgcgtggac gtcaggatgc
      301 gatcaaggac gacgtggaac gtgtcgacgc tctcgaccag gtcgtcacgg cctgccgccc
      361 ccagggcatc ccgtcgatgc ttctcgacgg agtgcttgca cctatcgagg tggaggcgac
      421 ccgcatcctc aacgaggtcc ccggtggcga agggatggcg ctgcgcttcg agcaggcacg
      481 cgctctcaag tcgcgtgtcg gagcccgtga agtcctcgac atcgtggtga cgctgccttc
      541 cggggtggaa cgtcccatcg agtcgctgtc cgccggtgag tccgttcgcg tctccctgtc
      601 gctcgtcttc gcgatgatct ctgtcctggg tgcacgccgc ggtggcgacg ccttggacac
      661 cgtgttcctt gacgagccgt tgggcccgct cgaccaggag gctgtgcccg cattcgtgca
      721 ggtgctgcgc tcggccgtca ccgcaggctc cttgtcgtcg gtcatggtcg tcacgcacga
      781 ccagcgcgtg attgacgctc tgccccagcg catctgggtg gagcgttcgg aggtgggtga
      841 ttccgttgcc accatcacca cgtagcggct tcaagtcgga cctgtccgaa ctgcctatgc
      901 gagacctccg tgccctgctg tcgcagacgc gtcgggagat ctcgaaacgc caactcgacg
      961 agggcaacca ggaccccgaa ctgctcgcgc aggcggcgtt gcagtcgctg ttcaccgaaa
     1021 cgtccgtggg cgctccgaag gttgtcgcac ctggcgtgct ggccctcggc ggtcaggttc
     1081 aagagcacgc accaggcagc acccgtcaca agtgccagtt gttcacggtc ttgcctgacg
     1141 gcgagggctc cacagagatg tgggcgtggg acgactcgat cgagacgttc atgtattccg
     1201 agaccgccaa ggtggccacc cgccgccggt cggtcgcgct tcacgcagtc accgagggcg
     1261 ccattgtcgt gcgccactca atgacgcacg acgggttccg acacaaccgc acggcagtgg
     1321 ccgcgtggag agtctcatcg gttgacgacg atggcaacgc tcgtatcgag cagttgtccg
     1381 ggtgggtgcc ctcaacgctt ccactttcac atgacacatc gggggattac cgctaatgag
     1441 caacaagcca cggctcgtag agatcaacac cttcttcgac gagatgcccg agagcaacat
     1501 cgaacacgag acgaagggcg ggcgccttgc gacgctactg gtcggtgccg tcgcgttcct
     1561 cgcgatcgtg ctgatcgtcc cgacgatcat cgttctgccg atggcgttcg ctatcgacgc
     1621 ggtgatgcag gaagcgcgcg gcgcctggca cgcggtgccc gcgaccgtcc ccgaagtccc
     1681 gtcgacgcta cccggcttca cgtacgtgta cgacagtgcg ggcaacgtcg tcgcccagtt
     1741 ctacgccgag aaccggcagc ctgtcacatt cgacgaagtg tccccactgc tggtcgacgc
     1801 gatcgtgtcc gtcgaggacc gccgcttctg ggaccacaac ggggttgacg gcaactctgt
     1861 ggtccgcgca gcatacgcga actacattgc cggaggggcc gtgcagggag cctccacgct
     1921 gacgcagcaa ctggtcgaga acctgaaact cctcagtgcg ggctccgccg aggagcgcga
     1981 cctggccagg gcgcagaccc tcggcggcaa ggtcgtcgaa gcgaagacgg cactggcgat
     2041 ggaagcgcag atgtcgaaga acgagatcct tgaggcgtac atgaacgtgg tgtacttcgg
     2101 cgccggctcg tacggtgtcg ccgcggccgc cgaccgattc ttcggtgtcg acgttgcaga
     2161 cctctccgtc gcgcaagcag ccaccatcgc cggaatggtg cgtgagccct ccggtctgga
     2221 tccgtttcgg ttccccgagc gcgcgcagag ccgacgcgac accgttctgc tggcgatgag
     2281 ggaaacgggc gccatcacct tgtgggagta cgtgaccgcg accaacagcc ccttggacac
     2341 ctctggtggc aaccagcagc ccaacggctg caccacgtct gcctacccgt tctactgctc
     2401 catcgtgcgc gagcaactgc tggtcgacgg acggctcgga cgcacgcagc cgcaacgcga
     2461 caacgcgatc gcccaaggcc acctggctgt ctacaccgca ctggaccctg ccgtcatggc
     2521 ctccatcgac gcctcgatcg ccgccgcgtt cgacgacagc aatcgtgtcg ccacttccgt
     2581 agcagttgtc gagcccggca cgggcctcgt gaagggcttc ggccagaacc gtcagtgggg
     2641 cgacggcgag ggggagacgg aaattccgta cgtgctgtca ggcttccagc ccgggtccgc
     2701 cttcaaggcg gtcatcatgg ccgccgcgct cgagaacggc ttcaacgccc acaccgcaat
     2761 gtctactcgg tcc
//
LOCUS       DASRKH010000238         2570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_1321, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000238 DASRKH010000000
VERSION     DASRKH010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1321"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..841)
                     /gene="folP"
                     /locus_tag="VFC06_03915"
     CDS             complement(35..841)
                     /gene="folP"
                     /locus_tag="VFC06_03915"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HZL81070.1"
                     /translation="MGVLNVTPDSFSDGGRYLDADAAIARGVALARDGADIVDIGGES
                     TRPGAERVAADEELYRVLPVVTALIAEGVPVCIDTMRAATARAAVEAGACLINDVSGA
                     LADPRMAGAVASTGVDFVAMHWRAHSPQMDDLDHYDDVVAEVASELAARVDALMAAGV
                     DPDRIVLDPGLGFAKVSDSNWPLLARVEEWSAGHRVLIGASRKRFLGAAISRDGNVDP
                     AAELREHATTAITTLAAAAGVWAVRVHDARAACDAIAVVCAWQDAAKATR"
     gene            complement(896..1468)
                     /gene="folE"
                     /locus_tag="VFC06_03920"
     CDS             complement(896..1468)
                     /gene="folE"
                     /locus_tag="VFC06_03920"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HZL81071.1"
                     /translation="MSEGIDTDRIEAAVREILVAIGEDPDRDGLRDTPARVARSYEEF
                     FAGLSQNPADVLSTVFELGHEEMILVKDIELYSMCEHHLVPFHGVAHVAYIPGTDGRI
                     TGLSKIARLVDVFAKRPQVQERLTTQVADALVDELGARGVLVVVEAEHLCMSMRGVRK
                     PGSRTVTSAVRGVMRDGATRAEAMSLIVGK"
     gene            complement(1478..>2570)
                     /locus_tag="VFC06_03925"
     CDS             complement(1478..>2570)
                     /locus_tag="VFC06_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZL81072.1"
                     /translation="MDGFDVSTNVIMIAATNRPDILDPALLRPGRFDRQIGIDAPDLK
                     GRLKVLEVHAKGKPIAPEVDLSAVAKRTPGFTGADLANVLNEAALLTARRSATAIGAS
                     ELDEAIDRVIAGPQKRTRVMNDHDKAVTAYHEGGHAVVAAAMNYTDPVTKVTILPRGR
                     ALGYTMVMPVEDRYSKTRNQLLDNLAYAMGGRVAEEVVFGDPSTGASNDISQATEIAK
                     QMVTEFGMSTRIGSVRLAGASGEVFLGRDMGHGRDYSDHLASVVDDEVRKLMDHAMEE
                     ATKALQANRGVLDTLAEELLEKETLTEAELAVLFKKIKKAPERAKWISGEWAPKRTPK
                     RRIAKAPVRPITEPKDTVADVATDLSNKA"
BASE COUNT          379 a          896 c          856 g          439 t
ORIGIN      
        1 gcgcatgtaa ggcgtcgaca tccaggtcat ggcttcatcg tgtcgccttt gcggcatctt
       61 gccaagcgca aacgaccgcg atcgcgtcac acgccgcacg cgcatcgtgc acgcgcaccg
      121 cccacactcc ggcggcagcc gcgagcgtcg tgatcgccgt cgtcgcgtgc tcccgaagtt
      181 ctgcggcagg gtccacattg ccgtcgcgcg agatcgccgc acccaggaaa cgcttgcgcg
      241 acgcgccgat aaggactcgg tgcccggccg accactcctc cacccgcgcc agcagcggcc
      301 aattcgagtc cgagaccttc gcgaatccga gccccgggtc gagcacgatg cgatccgggt
      361 ccacgcccgc agccatcagg gcgtcgacgc gggcggcgag ctccgacgcg acctcggcca
      421 ccacgtcgtc gtagtggtcg agatcatcca tctgcgggct gtgggcgcgc cagtgcatcg
      481 ccacgaagtc gacgcccgtg ctcgcgaccg cgccggccat gcgcgggtcc gcgagggctc
      541 cggagacgtc gttgatgagg caggcgcccg cctccaccgc ggcccgagcg gtcgcggccc
      601 gcatcgtgtc gatgcacacc ggcacgccct cggcgatgag agcggtcacg acgggcagca
      661 cccggtacag ttcctcgtcc gccgcgacgc gctccgctcc tgggcgggtg gactccccgc
      721 cgatgtcgac aatgtcggcg ccgtcgcgag ccaaggccac gccgcgggcg atcgcggcat
      781 cggcgtccag atagcggccg ccgtcgctga acgagtcagg ggtgacgttg agaacgccca
      841 tgacaagggt gcggtctgcc acgggaagtc cgggcgtctg gcgcgccggc atcggctact
      901 tcccgacgat gaggctcatg gcctcggcac gcgtggcgcc gtcgcgcatc acgccgcgca
      961 cggcggaggt cacggtgcgg gacccgggct tgcgcacgcc acgcatcgac atgcacaggt
     1021 gctccgcctc gaccacgacg agcacgccgc gggcgccgag ctcgtcgacc agggcgtcgg
     1081 ccacttgcgt ggtgaggcgc tcttgaacct gcggacgctt ggcgaacaca tccacgagcc
     1141 gcgcgatctt ggacagcccc gtgatgcggc cgtcggttcc cggaatgtag gccacgtgcg
     1201 cgacgccgtg gaagggcacc aggtggtgct cacacatcga atacagctcg atgtccttga
     1261 ccaggatcat ctcctcgtgg cccaattcga acacggtgct cagcacgtcc gcgggatttt
     1321 gggacaggcc ggcgaagaac tcctcgtagg agcgcgccac gcgtgccggc gtgtcccgca
     1381 ggccgtccct gtcgggatcc tcgccaattg ccacgagaat ctcgcgcacg gccgcctcga
     1441 tgcggtcggt gtcgatgccc tcactcatgc gacgttccta agccttgttc gacaagtcgg
     1501 tggcaacatc cgcgacggtg tccttgggct cggtaatggg ccgcacgggc gccttcgcga
     1561 tacgacgctt gggggtgcgc ttgggcgccc attccccact gatccacttg gcgcgctcgg
     1621 gagccttctt gatcttcttg aagagcacgg cgagttcggc ctcggtgagc gtctccttct
     1681 cgaggagttc ctccgcgagc gtgtcgagca cgccccggtt tgcctggagt gccttggtgg
     1741 cctcctccat ggcgtggtcc atgagtttgc gcacctcgtc atccaccacg gaggcaaggt
     1801 ggtcggagta gtcgcgtccg tgtcccatgt cgcgaccgag gaagacctcg ccagaggcgc
     1861 cagcgagccg caccgagcca atcctcgtcg acatgccgaa ctcggtcacc atctgcttgg
     1921 cgatctccgt ggcctgcgag atgtcgttgc tcgcgcccgt cgacgggtct ccgaacacga
     1981 cttcctcggc cacacgaccg cccatggcgt acgccaggtt gtcgagcaac tggttgcgcg
     2041 tcttcgagta gcggtcctct accggcatca ccatcgtgta gcccaaagct cggccgcgcg
     2101 gcaggatcgt caccttggtg acggggtccg tgtagttcat ggctgccgcc accacggcgt
     2161 ggccgccctc gtgatatgcc gtcaccgcct tgtcgtgatc gttcatcacc cgcgtgcgct
     2221 tctgtgggcc ggcgatgacg cggtcgatgg cctcgtccaa ttcgctcgcg ccgatcgcgg
     2281 tggcactgcg tcgcgccgtc agcagggctg cctcgttgag tacgttcgca aggtccgcgc
     2341 ccgtgaagcc gggcgtgcgc ttggcgaccg cgctgaggtc gacctccggc gcgatgggct
     2401 tgcccttggc gtgcacctcg agcaccttga gccggccctt gaggtcgggg gcgtcgatgc
     2461 cgatctggcg gtcgaagcgt cccggccgca acagcgccgg gtcgaggatg tcggggcggt
     2521 tggtcgcggc aatcatgatg acgttggtgc tgacgtcgaa gccgtccatc
//
LOCUS       DASRKH010000239         3145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_10616, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000239 DASRKH010000000
VERSION     DASRKH010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10616"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2969
                     /gene="rpoC"
                     /locus_tag="VFC06_03930"
     CDS             <1..2969
                     /gene="rpoC"
                     /locus_tag="VFC06_03930"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HZL81073.1"
                     /translation="TTTNSPVGMVLDAVPVIPPDLRPMVQLDGGRFATSDLNDLYRRV
                     INRNNRLKRLLDLGAPDIIVNNEKRMLQEAVDALFDNGRRGRPVTGPGNRPLKSISDM
                     LKGKQGRFRQNLLGKRVDYSGRSVIVVGPQLKLHQCGLPKQMALELFKPFVMKRLVEL
                     NHAQNIKSAKRMVERSRPEVWDVLEEVIREHPVLLNRAPTLHRLGIQAFEPQLVEGKA
                     IHLHPLVCAAFNADFDGDQMAVHLPLSSEAQAEARVLMLSSNNILKPSDGRPVTMPSQ
                     DMIIGLYHLTLLKEGSKGEGLAFGSESEGLMALDSGIIDLGTAITLRVDGDTVPPAGW
                     VAPDGWTEGEAMSLKTTLGRAIFNGLLPISFPYVNRDVDKKVLADIVNTLAERYEKVE
                     VAASLDALKAAGFHWATRSGVTIAITDVATPANKQEILDRYETLAAKVQDQYETGLIT
                     NPERRQELIEIWTRATNEVDVEMRKSFPKYNTVQTMVGSGARGNWMQVRQIAGMRGLV
                     SNPKGEIIPRPIKSNYREGLSVLEYFIATHGARKGLADTALRTADSGYLTRRLVDVSQ
                     DVIVREHDCGTERGLTMPIIERDAAGHTQWHERVATSVFARTIATDVVGADGTVLAKA
                     GVDAGDVLIDALIAAGVEEVKVRSVLTCESAVGTCALCYARSLATRELVDIGEAVGII
                     AAQSIGEPGTQLTMRTFHTGGVASAEDITQGLPRVQELFEARTPKGEAPIAEVAGRLK
                     VDDSEAMRRLVITPDSGDEEIAYPVTKRSRLLVQDGDHVEVGQQLVFGAVDPKNVLRI
                     LGPRATQKHLVDEVQQVYRSQGVEIHDKHIEVIVRQMLRRVTVLEAGETNLLPGELAE
                     RARFERLNRETVTKGGKPASARPELMGITKASLATESWLSAASFQETTRVLTEAALSG
                     KSDPLLGLKENVILGKLIPAGTGLHRYRGATVEPTEEAKSNLFPTFGYEEWDGQFFSE
                     GTGEAVRLEEFNFPRD"
BASE COUNT          557 a         1015 c         1025 g          548 t
ORIGIN      
        1 tgacgacgac gaacagcccg gtgggcatgg tcctggacgc cgtcccggtc atccctccgg
       61 acctgcgtcc gatggtgcag ttggacggtg gccgcttcgc gacctccgac ctcaatgacc
      121 tgtaccgccg tgtcatcaac cgcaacaacc gcttgaagcg gctgctcgat ctcggtgctc
      181 ccgacatcat cgtgaacaac gagaagcgca tgctgcagga ggctgtggac gcgctgttcg
      241 acaacggtcg tcgcggtcgc cccgtcacgg gtcccggaaa ccgtcccctc aagtccatct
      301 cggacatgct caagggcaag cagggtcgtt tccgtcagaa cctgctcggc aagcgtgtcg
      361 actactcggg ccgttcggtc atcgtggtcg gcccgcagct gaagctgcac cagtgtggtc
      421 tgcccaagca gatggccctg gaactgttca agccgtttgt catgaagcga ctggtggagc
      481 tcaaccacgc gcagaacatc aagtctgcaa agcgcatggt cgagcgcagc cgcccggagg
      541 tgtgggacgt cctcgaagag gtcatccgcg agcaccccgt actgctgaac cgcgcgccga
      601 cgcttcaccg cctgggcatc caggcgttcg agcctcagct cgtggagggc aaggccatcc
      661 acctgcaccc gctcgtgtgt gccgcgttca acgccgactt cgacggtgac cagatggcag
      721 ttcacctgcc gctgagctcc gaggcgcagg ccgaggcccg cgtcctcatg ctgtcgagca
      781 acaacatcct gaagccgtcg gacggccgac cggtgaccat gccttcgcag gacatgatca
      841 tcggcctgta ccacctgacg ctgctcaagg aaggctcgaa gggcgaggga ctcgcattcg
      901 gctccgagtc agagggcctc atggctctcg actccggcat catcgacctc ggcacggcga
      961 tcactctccg cgtcgacggc gacactgtgc cgcctgctgg ctgggttgct cccgatggct
     1021 ggacggaggg tgaggcgatg agcctcaaga ccaccctcgg tcgcgcgatc ttcaacggcc
     1081 tgcttccgat ctcgttcccg tacgtgaacc gcgacgtcga caagaaggtt cttgccgaca
     1141 tcgtcaacac tctggcggag cgctacgaga aggttgaggt cgcggcttcg ctcgacgctc
     1201 tgaaggcggc cggcttccac tgggcgaccc gttcgggtgt cacgattgcc attaccgacg
     1261 tggccactcc tgccaacaag caggagatcc tcgatcgcta cgagacgctc gccgcgaagg
     1321 tgcaggacca gtacgaaacc ggtctgatca ccaacccgga gcgccgtcag gagctcatcg
     1381 agatctggac gcgtgcgacc aacgaggtcg acgtcgagat gcgcaagtcc ttcccgaagt
     1441 acaacacggt gcagacgatg gtcggctccg gtgctcgtgg taactggatg caggtgcgtc
     1501 agatcgccgg tatgcgtggc ctggtatcga accctaaggg tgagatcatc ccgcgtccga
     1561 tcaagtcgaa ctaccgcgag ggcctgtcgg ttctcgagta cttcatcgca acgcacggtg
     1621 cccgcaaggg cctggccgac accgcgctgc gtaccgccga ctcgggttac ctgacccgac
     1681 gtttggtgga cgtgtcgcag gacgtcatcg tgcgtgaaca cgactgcggg accgagcgtg
     1741 gtctgaccat gccgatcatc gagcgtgacg cggcgggcca cacgcagtgg cacgagcgcg
     1801 tggcgacgtc ggtgttcgca cggacgattg ccacggacgt agtcggcgcg gatggcacgg
     1861 tgctcgcgaa ggcgggtgtc gacgccggtg acgtgctcat cgacgcgctg atcgctgcgg
     1921 gtgtcgaaga ggtcaaggtc cgttcggtcc ttacctgtga gtctgcagtg ggcacgtgtg
     1981 ccctctgcta cgctcgctcg cttgcgacgc gtgagctcgt cgacatcggc gaggccgtgg
     2041 gtatcatcgc ggcccagtcg atcggtgagc ccggcacgca gttgacgatg cgtaccttcc
     2101 acaccggtgg tgttgcctct gcggaggaca ttacgcaggg tctgccccgc gtccaagagc
     2161 tgttcgaggc ccgcaccccc aagggtgaag ccccgatcgc ggaggtcgca ggccgtctca
     2221 aggtcgacga ctcggaggcc atgcgccgcc tggtcatcac gcccgacagc ggtgacgaag
     2281 agatcgcgta cccggtcacc aagcgttcgc gcctgctggt gcaggacggc gaccacgtcg
     2341 aggtgggaca gcagttggta tttggtgctg tcgacccgaa gaacgtgttg cgcatcctcg
     2401 gaccgcgtgc cacccagaag cacttggtgg acgaggtcca gcaggtatac cgctcgcagg
     2461 gtgtggagat ccacgacaag cacatcgagg tcatcgtgag gcagatgctg cgtcgcgtga
     2521 ccgtgcttga agccggtgag accaacctgc ttccgggtga gcttgcggag cgcgcgcgct
     2581 tcgagcggct caaccgcgag acggtcacca agggcggcaa gcccgcctcg gcccgtccgg
     2641 agctcatggg tatcaccaag gcgtcgcttg cgaccgaatc gtggctgtcc gccgcatcgt
     2701 tccaggagac cactcgcgtc ctcaccgagg cggcgctgtc cggcaagtcg gacccgctgc
     2761 tcggcctcaa ggagaacgtg atcctcggaa agctgatccc cgccggcacc ggcctgcacc
     2821 gctaccgcgg tgccacggtc gagccgaccg aggaagccaa gtccaacctc ttcccgacgt
     2881 ttgggtacga ggagtgggac ggtcagttct tctcggaggg caccggcgag gccgtgcgtc
     2941 tcgaggagtt caacttcccg cgcgactagt cgcaactgcg cgttggcccc cgccgctcat
     3001 gacgagcgac gggggccttc gtgcgttctg cggcgattct tcacctcgcg ggagtgacat
     3061 aggggacatg cagttcactg acagttgcgg ctgtgcggac gacggggagg gcgacaccgt
     3121 cgatcaggcc cgactctcac aggtc
//
LOCUS       DASRKH010000240         2558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_4636, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000240 DASRKH010000000
VERSION     DASRKH010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4636"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2265)
                     /locus_tag="VFC06_03935"
     CDS             complement(<1..2265)
                     /locus_tag="VFC06_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HZL81074.1"
                     /translation="MKAANKAQSDTGLGDYVDRLRFPADPWRLLETSFGSSDIGTSET
                     LFSVGNGYLGFRGNVEEGRDFHSHGTFINGFHETWNIHHAEEAYGFARTGQTIVNVPD
                     AKVIRLYVDDEPLTLSVADLIEYDRSLDMRDGVLTRDLLWRTPAGNRVRVRSRRMVSF
                     TDRHVAVMTFDVMLLDKEAPIAISSQLLNREAGLDEYRANPDPTGATDPRRADAFQGR
                     VLEPRVHKEEDGRIHLGYECANSGMTMAVAMEHTVDYDGDYECHSEVDEDYGKHLFRT
                     QLKPGETFSLTKLVSYHTSRGVSARELADRCNRTLDRVSDEGADKQFEEQREWLDDFW
                     ARSDVEVGGDAGLQQAIRWSLFQLAQAGARGDGNGISAKGVTGSGYGGHYFWDAEVYV
                     VPFFTYTSPVMARNALRYRYIMLKPAEQRAAELAQRGALYPWRTINGEEASAYYAAGT
                     AQYHIDADIAYAIDKYVRATGDDEFLAREGIDILVQTARMWADLGFWRKTAGNEPSFR
                     IHGVTGPDEYTTVVNDNLYTNVMARFNLRCAAESVRKLADTWPDQHAKMVARLGLAQE
                     EIEEWERAAEAMAILWDENLGIHPQDALFNEREVWDLANTPLEKRPLLLHFHPLVIYR
                     FQVLKQADVVLALFLQGEHFSLEEKLSDFEYYDPITTGDSSLSGVVQSIIAAEVGYHE
                     LALKYFWDGVFVDIGNLHSNTSDGVHVASAGGAWSALVYGFGGLRDHGAMLHFDPRLP
                     DAWPSLTFRLTQRGT"
     gene            complement(2262..>2558)
                     /locus_tag="VFC06_03940"
     CDS             complement(2262..>2558)
                     /locus_tag="VFC06_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HZL81075.1"
                     /translation="LKVAGLADRFEVVVDGNVSAAEGLPGKPAPDTYQYAAKLLGVAD
                     AVSVVVEDAISGVQAGAAGDFALVIGVDRGAGADDLVRAGADIVVGDLAELVMP"
BASE COUNT          438 a          840 c          825 g          455 t
ORIGIN      
        1 ggtaccgcgc tgcgtcagcc ggaaggtgag cgacggccac gcgtcgggaa ggcgcgggtc
       61 gaaatgcagc atcgccccgt ggtcgcgaag cccgccgaag ccatacacga gcgccgacca
      121 cgccccgccg gccgatgcga cgtgcacgcc gtccgacgtg ttggagtgca ggttgccgat
      181 gtcgacgaac acgccatccc agaagtactt gagagcgagt tcgtggtaac ccacctcggc
      241 cgcaatgatg gactggacga cgccggacag ggacgagtcg cccgtggtga tcgggtcgta
      301 gtactcgaag tcggagagct tctcctccag cgagaagtgc tcaccctgga ggaagagcgc
      361 gagcaccaca tcggcctgct tgagcacctg gaagcggtag atgacgagtg ggtggaagtg
      421 gagcagcaac gggcgcttct ccagcggggt gttcgccagg tcccacacct cgcgctcgtt
      481 gaacagcgcg tcctgagggt ggatgccaag attctcgtcc cacaggatgg ccatggcctc
      541 cgcggctcgc tcccactcct cgatctcttc ttgagccagg cccaggcgcg ccaccatttt
      601 ggcgtgctgg tctggccagg tgtcggccag tttgcgcacc gactcggccg cacaacgcag
      661 gttgaagcgt gccatcacgt tcgtatacag gttgtcgttg accaccgtcg tgtactcgtc
      721 cggccccgtc acgccgtgaa tgcggaacga cggctcgttg ccggcggtct tgcgccagaa
      781 tccgaggtcg gcccacatcc gggcggtctg caccaagatg tcgatgccct cgcgcgccaa
      841 gaattcgtcg tctccggtcg cgcgcacgta cttgtcgatc gcatacgcga tgtcggcatc
      901 gatgtggtat tgcgccgtgc cggccgcata atacgcggag gcctcctcgc cattgatggt
      961 gcgccacgga tacaaggcgc cgcgttgggc gagctcggcc gcccgctgtt cggccggctt
     1021 gagcatgatg tagcggtatc tgagcgcgtt gcgcgccatc accggggacg tgtaggtgaa
     1081 gaacggaacc acgtagacct cggcgtccca gaaatagtgg ccaccatatc cggagccggt
     1141 cactcccttg gcggagatgc cgtttccgtc gccgcgcgcg cctgcttgag cgagctgaaa
     1201 cagggaccac ctgatggcct gctggagtcc cgcgtcgccg ccgacctcga cgtctgagcg
     1261 cgcccagaag tcgtcgagcc actcgcgctg ctcctcgaac tgcttgtccg ctccttcgtc
     1321 gctcacgcgg tcgagcgtgc ggttgcagcg gtcggccaac tcgcgcgccg acaccccgcg
     1381 cgacgtgtgg tagctgacta acttggtcag ggagaaggtc tcaccgggct tgagttgggt
     1441 tcggaacagg tgcttgccat agtcctcatc gacctcgctg tggcactcgt agtcaccgtc
     1501 gtagtcgacc gtgtgctcca tggccacggc catcgtcatg ccggaattgg cacattcata
     1561 gcccaggtgg atgcgcccgt cttcctcctt gtgcacgcgc ggttcgagaa cccgcccctg
     1621 gaaggcgtcc gccctacggg ggtccgtcgc gcccgtggga tccgggttgg cacggtattc
     1681 gtccaggcca gcctcgcgat tgaggagctg agaggagatg gcgatgggcg cctccttgtc
     1741 aaggagcatc acgtcgaacg tcatgaccgc aacgtggcga tccgtgaagg acaccattcg
     1801 ccgcgaccgc accctcaccc tgttgccggc gggcgtgcgc cacaacaggt cgcgggtgag
     1861 aaccccgtcg cgcatgtcga gcgaccggtc gtactcgatc aggtccgcga cagacagcgt
     1921 caggggttcg tcgtcaacgt agagcctgat gaccttcgca tcgggaacgt tgacgatcgt
     1981 ctggcctgtg cgcgcgaagc cataagcctc ttcggcgtga tggatgttcc atgtctcgtg
     2041 aaagccgttg atgaaggtgc cgtgagagtg gaagtcgcgg ccctcctcga cgttgccgcg
     2101 aaagccgagg tagccgttgc ccaccgagaa cagggtctcg ctggtgccga tgtcgctact
     2161 gccaaagctg gtttccagga gccgccacgg gtcggcagga aatcgcagcc gatccacgta
     2221 gtcgcccaac ccggtatcgg attgcgcctt gtttgcggcc ttcatggcat gaccaactcc
     2281 gcgaggtctc ccaccacgat gtcggcgcca gcccgaacca ggtcgtcggc gcccgctccc
     2341 ctatccacgc cgatcaccaa ggcaaagtca ccagccgcgc cggcctggac gcccgagatg
     2401 gcgtcctcca ccacgacgga caccgcgtcg gccacgccca gcagcttcgc ggcgtactgg
     2461 taggtgtcgg gcgcaggctt gccaggaaga ccctcggctg cggagacgtt gccgtccacc
     2521 actacctcga aacggtcggc gaggcccgcg accttgag
//
LOCUS       DASRKH010000241         2622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_62413, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000241 DASRKH010000000
VERSION     DASRKH010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62413"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..1532)
                     /locus_tag="VFC06_03945"
     CDS             complement(138..1532)
                     /locus_tag="VFC06_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HZL81076.1"
                     /translation="MTSTSPDAVPYPLVVRTVALDDLAHPGPPAVGAHAGTDDATDLL
                     DLLPADAPLAWVRRGDGLVGWGEALRITVTGPDRFADAEHAWHDVLAHAVVRDEVGLP
                     GTGPVAFGSFAFDEDSAAGGVVVVPRVVVGRRAGRMWLTTIDSGATLRPAPTLADVAG
                     TRTPVPSPGAVVYGDGAVPADRWPDVVGLGIAAIRAGELDKVVLARDVCARTTDPLDV
                     RWPLRRLAQQYPSCWTFSVDRMLGATPELLVRSEKGLVTSRVLAGTIRRTGDENADVA
                     HAAILAHSSKDLEEHEYAVASVAHALEPFCTSINVPDVPFVLHLPNVLHLASDVTAVL
                     AGSAPDGPGTSPGAAGLAGSTGVHPSSLALAAALHPTAAVCGTPTDAARDLIARIEGM
                     DRARYAGPVGWFGADGDGEWGIALRSAQVDPDDPRRIRLFAGCGIVAASDPAAELAES
                     EAKLVPMRDALASD"
     gene            1592..2287
                     /locus_tag="VFC06_03950"
     CDS             1592..2287
                     /locus_tag="VFC06_03950"
                     /EC_number="2.1.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="demethylmenaquinone methyltransferase"
                     /protein_id="HZL81077.1"
                     /translation="MPRATLDKDPRAVAAMFDAVARRYDVTNDVISLGQDRYWRRATL
                     AAVGAQRGEQVLDLAAGTGTSSEPLADAGVRVVPCDLSFGMLEVGKRRRPDLGFVAGD
                     ATALPFADESFDAVTISFGLRNVVDTAGALAELLRVTRPGGRLVVCEFSRPTWAPFRT
                     VYTEYLMRALPPVARAVSKEPDAYVYLAESIREWPDQQGLGRLIKAAGWGQVAFRNLS
                     GGIVALHRGVRPA"
     gene            2438..>2622
                     /locus_tag="VFC06_03955"
     CDS             2438..>2622
                     /locus_tag="VFC06_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZL81078.1"
                     /translation="MGKSVVNAGHDDADVIVVGAGPAGSSAAYHCASAGLDVLLLDKA
                     SFPRDKICGDGLTPRAV"
BASE COUNT          343 a          920 c         1015 g          344 t
ORIGIN      
        1 ggccgggtgt cctcccttcg ccccccggga gacggtcgat gcggaccccg catccgcgtc
       61 ccgtcacagg tacggccggc accctcgggt gccggccgta ccttcgtgcg ccgctcgggg
      121 cgtgcgggtg cgtggtgtca gtcggacgcg agcgcgtcgc gcatgggcac gagcttggcc
      181 tcggactcgg cgagctcggc agcagggtcg gacgcggcga cgatgccgca cccggcgaac
      241 agccggatgc gccgcgggtc gtcggggtcc acctgcgccg accgcagcgc gatgccccac
      301 tcgccgtcgc cgtcggcgcc gaaccagccg accggtccgg cgtaccgtgc gcggtccatg
      361 ccctcgatcc tggcgatgag gtcgcgcgcg gcgtccgtgg gggtcccgca caccgcagcg
      421 gtcgggtgca gcgccgcggc gagcgcgagg ctcgacgggt ggacgcccgt ggatccggcg
      481 agccctgcgg cgcccggcga cgtgcccgga ccgtcgggag ccgagccggc cagcacggcc
      541 gtgacgtccg aggcgaggtg caggacgttc ggcaggtgca ggacgaacgg cacgtcgggc
      601 acgttgatcg aggtgcagaa cggttcgagg gcgtgcgcga cggacgcgac cgcgtactcg
      661 tgctcctcga ggtccttcga ggagtgcgcg aggatcgcgg cgtgcgcgac gtcggcgttc
      721 tcgtccccgg tgcgccggat ggtcccggcg agcacgcgcg aggtgacgag ccccttctcg
      781 gagcgcacga gcagctcggg cgtcgcgccg agcatcctgt cgaccgagaa cgtccagcac
      841 gacgggtact gctgggcgag gcggcgcagc ggccagcgca cgtcgagcgg atccgtggtg
      901 cgtgcgcaga cgtcgcgcgc gagcacgacc ttgtcgagct cacccgcgcg gatcgcggcg
      961 atgcccagtc cgacgacgtc cggccagcga tccgccggga cggcgccgtc gccgtacacg
     1021 acggcgcccg gcgaggggac gggtgtgcgg gtgccggcca cgtcggcgag cgtgggcgcg
     1081 gggcgcaggg tcgcgccgga gtcgatcgtc gtgagccaca tgcggcccgc gcgccgtccg
     1141 acgacgacgc gcggcacgac gacgacgcca cccgcggccg agtcctcgtc gaacgcgaac
     1201 gacccgaacg cgaccggtcc ggtcccgggc aggccgacct cgtcgcgcac gaccgcgtgc
     1261 gcgagcacgt cgtgccaggc gtgctcggcg tccgcgaacc ggtcgggccc cgtcacggtg
     1321 atccgcaggg cctcacccca cccgacgagc ccgtcgcccc gccggaccca cgcgagcggg
     1381 gcgtcggcgg gcagcaggtc gagaaggtcg gtggcgtcgt cggtaccggc atgggcgccg
     1441 acggccgggg ggccggggtg ggcgaggtcg tcgagcgcaa ccgtccgtac gaccaaggga
     1501 tacgggacgg catcgggcga ggtgctggtc atcgtgtgcc agggtacgtc ccccggacgc
     1561 gacacccctg ccgacctgag acgatgtggt catgccccgc gccaccctcg acaaggaccc
     1621 ccgtgccgtc gccgcgatgt tcgacgccgt cgcgcgccgc tacgacgtga cgaacgacgt
     1681 catctccctc gggcaggacc gctactggcg ccgggcgacc ctcgctgcgg tcggggcgca
     1741 gcgcggcgag caggtgctcg acctcgctgc gggcaccggg acgtcgagcg agccgctcgc
     1801 cgacgccggc gtgcgcgtcg tgccgtgcga cctgtcgttc gggatgctcg aggtcggcaa
     1861 gcgccggcgc cccgacctgg ggttcgtcgc gggcgacgcg accgcgctgc cgttcgcgga
     1921 cgagtcgttc gacgccgtga cgatctcgtt cggcctgcgc aacgtcgtgg acaccgcggg
     1981 cgcgctcgcg gagctcctgc gcgtcacccg gcccggcggg cgtctcgtgg tgtgcgagtt
     2041 ctcgcgcccg acgtgggcgc cgttccggac ggtctacacc gagtacctca tgcgcgcgct
     2101 gccgcccgtc gcgcgggcgg tgtccaagga gcccgacgcc tacgtctacc tggccgagtc
     2161 gatccgtgag tggccggacc agcagggcct cgggcggctg atcaaggccg ccggatgggg
     2221 tcaggtcgcg tttcggaacc tttcgggcgg gatcgtggca ctgcatcgcg gtgtgcgtcc
     2281 ggcgtgagac cgaggggccc gctgaggtgc gcgcacgagg cgtgacgggt gatgtgagca
     2341 gggtcacggc tgtccagcga tggtgaggta agccttcgca gacacgtcgt gggacggtgg
     2401 ttagactcgg caagtcgtag caggaaagcc ttcacaaatg gggaagtccg tggtcaacgc
     2461 agggcacgat gacgctgatg tcatcgtcgt cggcgctggc cccgccgggt cgagcgcggc
     2521 gtaccactgc gcatcggcgg gcctggacgt gctgctcctc gacaaggcgt cgttcccgcg
     2581 ggacaagatc tgcggcgacg gcctgacgcc ccgcgcggtc aa
//
LOCUS       DASRKH010000242         2375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_58319, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000242 DASRKH010000000
VERSION     DASRKH010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58319"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="VFC06_03960"
     CDS             complement(<1..606)
                     /locus_tag="VFC06_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005055596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HZL81079.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAISKVLHDKYPKIN
                     PFTPFADIDKAPEERERGITINIAHIEYETEKRHYAHVDAPGHADYIKNMITGAAQMD
                     GAILVVAATDGPMAQTREHVLLAKQVGVPYLMVALNKVDMIPAADEELLELVEMEVRE
                     LLSKQGFDGDNVPVIRVSALKALEGDPEWVAKVQELMDAVDE"
     gene            complement(731..>2375)
                     /gene="fusA"
                     /locus_tag="VFC06_03965"
     CDS             complement(731..>2375)
                     /gene="fusA"
                     /locus_tag="VFC06_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HZL81080.1"
                     /translation="GAKPLVMQLPIGSEGDFVGIVDLIEMNAKVWPGDSKGDVTLGAK
                     YEVQPIPADLQAKAEQYRAELLEAVADASDELLEKYLGGEELTIPEIKKGIRKMVLGS
                     ELYPVLCGSAFKNRGVQPMLDAVVDYLPNPMDMPAIEGHALGDVDKIVERHADAKEPF
                     AALAFKIMTHPFFGRLTYIRVYSGHANSGAALLNATKDKKERIGKIFQMHANKENPVD
                     FVTAGHIYAVIGLKDTTTGDTLCDAQHPVVLESMTFPEPVIHVAVEPKTKGDQEKMSL
                     AIQKLAEEDPTFRVRLDIDSGQTIISGMGELHLDIIVDRMRREFKVDANVGKPQVAYR
                     ETIRRPVMKYDYVHKKQTGGSGQFAKVQINLEPLPADSELQYEFVNAVTGGRVPREYI
                     PSIDAGIQDAMQLGIQAGYPVVGIRATLVDGAYHDVDSSEMAFKIAGSMVFKEASRKA
                     DPALLEPIMAVEVRTPEDYMGDVIGDLNSRRGQIRQMSDAVGVKVIDSLVPLSEMFGY
                     VGDLRSRTQGRAVYSMTFDSYAEVPRNVAEEIIAKTRGE"
BASE COUNT          401 a          730 c          737 g          507 t
ORIGIN      
        1 ctcgtccacc gcgtccatga gctcctgaac cttggcaacc cactcggggt cgccctcaag
       61 tgccttgagc gccgaaaccc ggatgacggg gacattgtcg ccgtcgaagc cctgcttcga
      121 gagaagttca cgaacttcca tctccacgag ctcgaggagt tcctcgtccg ccgcggggat
      181 catgtcgacc ttgttgaggg ccaccatcag gtacggcacg ccgacctgct tcgcgagcaa
      241 cacgtgctca cgcgtctggg ccatcgggcc gtcggttgct gcaaccacga ggattgcgcc
      301 gtccatctgg gcggcaccgg tgatcatgtt cttgatgtag tccgcgtgcc cgggtgcgtc
      361 gacgtgagcg tagtgacgct tctcagtctc gtactcgatg tgcgcgatgt tgatcgtgat
      421 accgcgctcg cgctcttcag gggccttgtc aatgtctgcg aaaggcgtga aggggttgat
      481 cttggggtac ttgtcgtgca gcaccttcga gatcgcggcg gtcagcgtcg tcttaccgtg
      541 gtcgacgtga ccgatcgtgc cgatgttcac gtgcggcttg gtccgctcgt acttggcctt
      601 agccatgctg gcttcctcct gtaaatatga gactcgggta gtgaataaga tacgcgcttg
      661 ggccgggggc cgtcgccggg gtcgtcttcc ctactggtcg ttattgtttc tgatctttcg
      721 accgttggga ctactcgccc cgggtcttgg caatgatttc ctcggcaacg ttccggggaa
      781 cctcggcgta gctgtcgaac gtcatcgagt acacggcacg tccctgggtg cgagaccgca
      841 ggtcgcccac gtagccgaac atctccgaca acggaaccaa agaatcaatg accttcacgc
      901 ccaccgcgtc cgacatctgt cggatctggc cacggcgtga gttgaggtcg ccgatgacgt
      961 cgcccatgta gtcctcgggc gtacgaacct cgacggccat gatcggttcg agaagtgcgg
     1021 ggtcggcctt cctggaggcc tccttgaaca ccattgagcc agcaatcttg aacgccattt
     1081 cagacgagtc aacgtcgtgg tacgcgccgt caaccagcgt ggccctgatg cccacgaccg
     1141 ggtatcccgc ttggattccc agctgcatgg cgtcttggat gccagcgtcg atcgacggga
     1201 tgtactccct cggcacgcgg ccgccggtca ctgcgttgac aaactcgtac tgaagctcgg
     1261 agtctgcggg cagcggctca aggttgatct ggaccttggc gaactgaccc gaaccaccgg
     1321 tctgcttctt gtgcacgtag tcatacttca tgacaggacg gcgaatcgtc tcgcggtacg
     1381 ccacctgggg cttacccacg ttggcgtcca ccttgaactc gcgccgcatg cggtcaacga
     1441 tgatgtcgag gtgaagctcg cccatgccgg aaatgatggt ctggccagag tcgatgtcga
     1501 gcctgacgcg gaacgtcggg tcctcttcgg ccaacttctg gatggccagc gacatcttct
     1561 cttggtcgcc cttggtcttg ggctccacgg ccacgtgaat cacgggctcg gggaacgtca
     1621 tcgactcgag cacgacgggg tgctgcgcgt cgcacaaggt gtcgcccgtg gtggtgtcct
     1681 tgaggccgat gaccgcgtag atgtggccag cggtcacgaa gtcgaccggg ttctccttgt
     1741 tggcgtgcat ctggaagatc ttcccgatgc gctccttctt gtccttggtg gcgttcagca
     1801 aggcggcacc ggagttggcg tggccggagt acacgcgaat gtacgtgagg cgaccgaaga
     1861 acgggtgcgt catgatctta aacgcgagag ccgcaaaggg ctcctttgcg tcggcgtgac
     1921 gctccacgat cttgtcgacg tcaccgaggg cgtgaccctc gatggcaggc atgtccatcg
     1981 ggttaggcag gtagtccacg accgcgtcga gcatgggctg cacgccgcgg ttcttgaacg
     2041 ccgagccaca caggacgggg tagagctcgg agccaagcac catctttcgg atgcccttct
     2101 tgatctccgg gatcgtcagc tcttcgccgc cgaggtactt ctcaagcaac tcatcgctcg
     2161 cgtcagccac tgcctcgaga agctcggcgc ggtactgctc ggccttcgcc tggaggtctg
     2221 cggggatcgg ctggacctcg tacttggcgc ccaaggtcac gtcgcccttg gagtcgcctg
     2281 gccacacctt ggcattcatc tcgatgaggt ccacgatgcc gacgaagtcg ccctctgaac
     2341 cgatcgggag ctgcatcacg agcggcttgg cgccg
//
LOCUS       DASRKH010000243         2126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_49201, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000243 DASRKH010000000
VERSION     DASRKH010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49201"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..587)
                     /locus_tag="VFC06_03970"
     CDS             complement(69..587)
                     /locus_tag="VFC06_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81081.1"
                     /translation="MIRADARRLAVVPDWAAGRGLVEVARHPASTPGNPRINLAHYPD
                     HPQNPDGTPAVPKPRAGSLLEQVFLSIGPAAELWLVAACAQGVERIESKMRSIVDLAA
                     LRGSLLACHALEAAVQAGRFAWGDVESIAEFIAATGTHDEAPVFAGEACSTQPGTGPW
                     AGFITTRIEVAR"
     gene            complement(584..1618)
                     /gene="istA"
                     /locus_tag="VFC06_03975"
     CDS             complement(584..1618)
                     /gene="istA"
                     /locus_tag="VFC06_03975"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HZL81082.1"
                     /translation="MEILEAFDKTGCAHSAAGLVGCDPKTVRRLVARREAGVGADALI
                     GRPRLVDGFTGVVEQLVDGSDGVIRADKAHEKLVACGYGGSERTTRRAVAAAKARWRL
                     EHARSNRPWITVPGMWCQFDWGDGPKVMDVNGRLRSTVLFVAWVAWSRFRVVIPCWDQ
                     TSQSLATCLDSMFRLVGGVSDYVLTDNAKTVTVQHIAGVPVRHPLMVDVGRHYGTTVH
                     TCVPYDPQSKGGVEASVKIAKDDLLPKKTNLRGEYASMDELAQACSEFMAKVNGRVHQ
                     ATRRRPVEALTAEREFLHPVPVEPHTAALGQARVVAPDQTVSFGSVRYSVPPKLQGAR
                     CGSRCRGTRS"
     gene            1774..>2126
                     /locus_tag="VFC06_03980"
     CDS             1774..>2126
                     /locus_tag="VFC06_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81083.1"
                     /translation="MTARTEINWPPTRRLRWPWTRGERTRRRRLTGALTASSALALGL
                     AMAAVPATATQPIRDVQQGTGINCELLPVGEGDTSLFLGAFFSWGGDVVDGFGEVVTP
                     AMAAWAYQGTGELDAG"
BASE COUNT          345 a          809 c          678 g          294 t
ORIGIN      
        1 cggcaggcgc atcctgcgca gggcggcatc gagggcgtcg ggcagggcgg gcgcactggt
       61 gtcggtgatc atcgggccac ctcgatccgg gtcgtgatga acccggccca aggcccggtc
      121 ccgggctggg tggagcaggc ctcgccggcg aacacgggtg cctcgtcgtg ggtgccggtg
      181 gcggcgatga actccgcgat cgactcgacg tcaccccaag cgaaccggcc ggcctgcacc
      241 gctgcctcca gcgcgtggca ggccagcagg gaaccgcgca gcgcggccag gtccactatg
      301 gagcgcatct tcgactcgat gcgctccacg ccctgcgcgc aggcggcgac cagccacagc
      361 tcggcggccg gaccgatcga gaggaacacc tgctccaaca acgacccggc ccgcggtttc
      421 gggaccgccg gggtgccgtc cgggttctgg ggatggtcgg gatagtgcgc caggttgatc
      481 cgcgggttgc ccggcgtgga cgccgggtgg cgggccacct ccaccagacc gcggccggcc
      541 gcccagtcgg gcaccaccgc caggcggcgg gcgtccgcgc ggatcacgac ctcgtccccc
      601 tgcaccgcga cccacaccgg gcgccctgca gcttcggcgg caccgaatag cgcaccgacc
      661 cgaacgagac cgtctgatcc ggggcgacca ccctggcctg ccccaacgcg gccgtgtgcg
      721 gctcgaccgg caccgggtgc aggaactccc gctccgccgt cagggcctcc accgggcggc
      781 ggcgggtggc ctggtggacc cgcccgttga ccttggccat gaactccgaa cacgcctggg
      841 cgagttcgtc catggaggcg tactcgccgc gcaggttcgt cttcttcggc aacaagtcgt
      901 ccttcgcgat cttcaccgac gcctccaccc cacccttgga ctgcgggtcg tacggcacgc
      961 acgtgtggac ggtcgtgccg taatgccggc ccacgtccac catcaacgga tgccgcaccg
     1021 gcacccccgc aatgtgctgc acggtcaccg tcttggcgtt gtcggtcaac acgtagtcgc
     1081 tcaccccgcc gaccagccgg aacatcgagt ccaagcaggt cgccaacgac tgtgacgtct
     1141 ggtcccagca cgggatcacc acccggaacc tggaccacgc gacccacgcc acgaacaaca
     1201 ccgttgatcg cagccggccg ttgacgtcca tcaccttcgg gccatccccc cagtcgaact
     1261 ggcaccacat ccccggaacc gtgatccacg gccggttcga gcgggcatgc tcaaggcgcc
     1321 accgggcctt ggccgccgcg accgcccgcc gggtcgtccg ctccgacccg ccatacccgc
     1381 atgccaccaa cttctcgtgg gccttgtccg cccggatcac accatccgac ccgtcaacca
     1441 gctgctccac caccccggtg aacccgtcca ccagccgcgg gcgtccgatc agggcgtccg
     1501 cgcccacccc ggcctcccgg cgggccacca gccgtcgtac cgtcttcggg tcacacccca
     1561 ccaggcccgc agcactatgc gcgcaccccg tcttatcgaa agcttccaag atttccatcg
     1621 tctccacacc gttcttcttc acgtgtcccc ggaccccttc cgcagtgcaa tcagcactgc
     1681 agggctaccc ggacaggcac ccggacacgg accgacacgc cgtcagtcca cgacacggca
     1741 gggagatcag ctggccacga aacgggagat cacgtgaccg ccagaacgga gatcaactgg
     1801 ccgcctacaa ggaggttacg gtggccatgg acacgcggtg agcggacacg cagacgcagg
     1861 ctcacagggg cactcacggc ctccagtgca ctggccctgg ggctggcgat ggcggccgtg
     1921 cccgcgacgg ccacacagcc catccgggac gtccagcagg gaactggcat caactgcgaa
     1981 ctcctaccgg tgggggaggg cgacaccagt ctcttcctcg gggcgttctt ctcatggggc
     2041 ggtgacgtcg tcgatggctt tggcgaggtg gtcacccccg cgatggccgc gtgggcgtac
     2101 cagggcaccg gtgagctgga cgccgg
//
LOCUS       DASRKH010000244         2205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_58759, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000244 DASRKH010000000
VERSION     DASRKH010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58759"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /gene="nuoK"
                     /locus_tag="VFC06_03985"
     CDS             complement(<1..292)
                     /gene="nuoK"
                     /locus_tag="VFC06_03985"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HZL81084.1"
                     /translation="MSLTNYLVLASILFAIGATTVLLRRNAIIVFMGVELMLNATNLL
                     LVTFSRLHGNLTGQVLAFFVMVVAAAEVVVGLAIIVAIFRTRRSASVDDVNLL"
     gene            complement(289..1221)
                     /locus_tag="VFC06_03990"
     CDS             complement(289..1221)
                     /locus_tag="VFC06_03990"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HZL81085.1"
                     /translation="MSVLATAVPAVLTGALPMSLTSAGVTTSAEAVLFWVLGPVMVLA
                     ALGLLFARKAVHAAMAVVVVMISLAFLYVAQDAPFLGVVQVVVYTGAIMMLFLFVLML
                     VGVDASDSLVETIRGQRWIGLLAGAGLGVVLVGVAVRATYPKAAGLQTPNADSNPVGI
                     ARTVFSDYAFALEVVGALLVIAALGALVLTHRQRLVPRIGQKERADSRVAKGERLTPL
                     PAPGVYARHNAMDVPALDPRGNPIEESVSRVLRVRGQEQGPRVLARLGSAATGTLVES
                     PHEDAPPPGAAPQGPPDALPVTRVDPATRTEEGL"
     gene            complement(1218..1865)
                     /gene="nuoI"
                     /locus_tag="VFC06_03995"
     CDS             complement(1218..1865)
                     /gene="nuoI"
                     /locus_tag="VFC06_03995"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HZL81086.1"
                     /translation="MAAPSAYKSLIEPRTGIAEAVAPVAGFGVTLSNMFRPTVTEQYP
                     FERVPTQPRYHGRHQLNRYADGLEKCIGCELCAWVCPADAIYVEGADNTPDEQFSPGE
                     RYGRVYQINYLRCIFCALCIEACPTRALTMTNEFELAGPTRAGLIFEKQDLLAPRGDG
                     MLAAPHPMVPNTTDKNYYRGEVTGTTPEQTAWVAEHRPDDPTLPAASTTAAGETR"
     gene            complement(1906..>2205)
                     /locus_tag="VFC06_04000"
     CDS             complement(1906..>2205)
                     /locus_tag="VFC06_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HZL81087.1"
                     /translation="QGVRQFSSLDLRSILFGLAGVVLLGVLVSFLIPEKKPQPGPGAG
                     GPPVVFDPFAGGYPVPPMPGQVLPPSPRAQRAAARAASVADQTETPQVTQEVHGG"
BASE COUNT          348 a          754 c          812 g          291 t
ORIGIN      
        1 tcaacaggtt gacgtcgtcg accgaggccg agcggcgggt tcggaagatg gccacgatga
       61 tcgccaggcc gacgacgacc tcagcggcgg cgacgaccat cacgaagaag gccagcacct
      121 gccccgtgag gttgccgtgc agtcgcgaga acgtcacgag cagcaggttc gtcgcgttca
      181 gcatgagctc gacgcccatg aacacgatga tcgcgttgcg gcgcagcagc accgtcgtcg
      241 cgccgatcgc gaacaggatc gaggcgagca cgaggtagtt cgtcaggctc acagcccctc
      301 ctccgttcgc gtggcgggat cgacccgggt gaccggcagt gcatcgggcg ggccctgcgg
      361 agccgcacca ggcggcggtg cgtcctcgtg gggcgactcg accagggtcc cggttgcggc
      421 cgaaccgagg cgcgcgagga cccgcggtcc ctgctcctga ccacggacgc gcagcacgcg
      481 cgagaccgac tcctcgatcg ggttgccccg cgggtcgagc gcgggcacgt ccatcgcgtt
      541 gtgccgcgcg tacacgccgg gggccggcag cggggtgagc cgctcaccct tcgcgacgcg
      601 ggagtcggct cgttccttct ggccgatgcg cgggacgaga cgctgacggt gcgtgaggac
      661 gagcgcgccg agcgccgcga tcaccagcag cgcgccgacg acctcgagcg cgaacgcgta
      721 gtcgctgaac accgtgcggg cgattcccac cgggttcgag tccgcgttgg gggtctgcag
      781 cccggcggcc ttcgggtacg tcgcccgcac ggccacgccg acgaggacga caccgaggcc
      841 ggcacccgcg agcaggccga tccagcgctg gccgcggatc gtctcgacga gcgagtcgga
      901 cgcgtcgacc ccgacgagca tgaggacgaa gaggaacagc atcatgatcg cgcccgtgta
      961 caccacgacc tggaccacac cgaggaacgg ggcgtcctgc gcgacgtaga ggaacgcgag
     1021 cgagatcatc acgacgacga ccgccatcgc ggcgtggacg gccttgcggg cgaacagcag
     1081 gcccagggcc gcgaggacca tgaccgggcc gagcacccag aacagcacgg cctcggccga
     1141 ggtcgtgacg ccggcgctcg tgagcgacat cggcagggcg ccggtcagca ccgccggaac
     1201 ggcggtcgcg agcacgctca tcgggtctcc cccgctgcgg tggtcgatgc ggcgggcagc
     1261 gtggggtcgt ccggtcggtg ctcggcgacc cacgccgtct gctcgggcgt cgtgcccgtg
     1321 acctcgccgc ggtagtagtt cttgtccgtg gtgttcggga ccatcggatg cggagccgcg
     1381 agcatcccgt cccccctcgg tgcgagcagg tcctgcttct cgaagatgag cccggcccgg
     1441 gtcgggcccg cgagctcgaa ctcgttggtc atcgtcagcg cgcgcgtcgg gcacgcctcg
     1501 atgcacagcg cacagaagat gcagcgcagg tagttgatct ggtagacgcg gccgtaccgc
     1561 tcgccgggcg agaactgctc gtcgggcgtg ttgtcggcgc cctcgacgta gatcgcgtcc
     1621 gcggggcaca cccaggcgca cagctcgcag ccgatgcact tctcgagccc gtccgcgtac
     1681 ctgttgagct ggtggcgacc gtggtaccgc ggctgggtgg gcacgcgctc gaacgggtac
     1741 tgctccgtga ccgtcggccg gaacatgttc gagagcgtga caccgaagcc ggcgaccggg
     1801 gcgaccgcct cagcgatccc cgtgcggggc tcgatgagcg acttgtacgc gctcggggca
     1861 gccacgtcac ctgccttcgg gcgaggcgtg acgtccttcg acgggtcagc caccgtgcac
     1921 ctcctgcgtg acctgcgggg tctcggtctg gtccgcgaca gaggcggccc gggcggccgc
     1981 gcgctgcgcg cggggggacg ggggaagaac ctggccgggc atgggcggga ccgggtagcc
     2041 acccgcgaac gggtcgaaca ccacgggtgg cccacctgcg cccgggcctg gttgtggctt
     2101 cttctccggg atgaggaagg acacgaggac gccgaggagc accacaccgg cgagcccgaa
     2161 caggatcgag cgcaggtcca ggctcgagaa ctgccggaca ccctg
//
LOCUS       DASRKH010000245         2213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_24413, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000245 DASRKH010000000
VERSION     DASRKH010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24413"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..975)
                     /gene="secF"
                     /locus_tag="VFC06_04005"
     CDS             complement(<1..975)
                     /gene="secF"
                     /locus_tag="VFC06_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HZL81088.1"
                     /translation="MASFAQFGNELYTGARSYNIVGRRKLWFTIAITGMLLSFLVLGT
                     KGINPGIEFRGGSEFTLTNVTSNSGDPARAVLAEAGVTEAARITTVGTDSLRIQTEEL
                     TADQIATVRQGLADAYDLDLTGVTSTFIGPTWGADVTSKAIQGLVIFLILVSVVMAAY
                     FRTWKMSASAVLALVHDLVITVGLYALVGFEMTPASVIGFLTILGYSLYDTVVVFDKV
                     RENTARLLDQDRVTYEEAANLALNQTLVRSINTSVVGILPVASILFVGTLLLGAGTLR
                     DIALALFIGMIVSTISSIFIATPLLVWLRNREDKVGNHTARVLRARAQR"
     gene            complement(975..>2213)
                     /gene="secD"
                     /locus_tag="VFC06_04010"
     CDS             complement(975..>2213)
                     /gene="secD"
                     /locus_tag="VFC06_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015771650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HZL81089.1"
                     /translation="FDYYVLDCTDPANLSGGDAASPAEAVIACAQNGSAKYVLGPADV
                     EGTQITKAASGLETTTTGATTNNWIVTMSFDAEGKQAFATATTRLMSLPSPQDQFAIV
                     LDGLVVSAPSVSNPIANGEAMISGTFTRESAATLANQLNFGSLPLDFEVQSEEQISAT
                     LGLEQLQRGILAGLIGLVLVLIYMLLQYHALAAVAAASLGVAAFITYLTITLLGWTTG
                     YRLSLAGVAGLIVAIGITADSFIVYFERIRDEVREGRGLSDAVEYGWARAKRTIFASD
                     GVNFLAAFVLYFLAVGGVRGFAFTLGLTTVVDLLVVMAFTHPVMRLLTRTRYFGEGSR
                     WSGLDPEHLGATRATYRGRGSFARPAPAAKVAATAEPTEAGEVPPSGRTGMTIAERRA
                     AEAARVKEGARVAAGKEDQD"
BASE COUNT          372 a          713 c          786 g          342 t
ORIGIN      
        1 gcgctgggcg cgtgcccgca gcacccgggc cgtgtggttg ccaaccttgt cctcgcggtt
       61 tcgcagccac acgaggagcg gcgtcgcaat gaagatcgac gagatggtgg acacgatcat
      121 gccgatgaac agggcgagcg cgatgtcccg cagcgtaccg gcccccaaca acagtgtccc
      181 gacgaagagg atcgaggcca cgggcaggat acccacgacc gacgtgttga tcgaacgcac
      241 cagcgtctgg ttgagggcga ggtttgcggc ctcctcgtag gtcacgcgat cctggtcgag
      301 cagccgggcg gtgttctccc tgaccttgtc gaagaccacc accgtgtcgt agagcgagta
      361 ccccaggatg gtgaggaacc cgatcacgga tgccggggtc atctcgaagc ccacgagcgc
      421 gtacaacccg accgtgatga ccaggtcgtg gaccagcgcg agtacggcag aggccgacat
      481 cttccatgtg cggaagtagg ctgccatgac cacggacacc aggatgagga agatgaccag
      541 tccctggatg gccttgctgg tgacgtccgc accccacgtg ggtccgatga atgtcgaagt
      601 caccccggtg agatcgaggt cgtaggcgtc ggcgaggccc tggcggaccg tggctatctg
      661 gtccgcggtg agttcctcgg tctggattcg cagcgaatcg gtgcccaccg tggtgatgcg
      721 cgcggcctcc gtgactcccg cctcggccag cacggcgcgg gcaggatcac cggagttcga
      781 ggtgacgttg gtaagcgtga attcggaccc cccccggaac tcgatcccgg ggttgatgcc
      841 cttggtgccg agcaccagga acgagagcag cattccggtg atcgcgatcg tgaaccagag
      901 cttgcgccgc ccgacgatgt tgtaggaacg cgcacccgtg tacagctcat tgccgaactg
      961 ggcgaaactg gccatcagtc ctgatcctcc ttgcctgccg ccacgcgtgc cccttccttc
     1021 acccgcgccg cctcagccgc gcgccgttcc gcgatcgtca ttccggtgcg gcccgacggc
     1081 ggcacctccc cggcctcggt gggctccgcc gtggccgcaa cctttgccgc cggcgcgggc
     1141 cgggcgaagg agccccggcc acggtaggtc gcccgggtcg cgccgaggtg ttcggggtcg
     1201 agcccggacc agcgactgcc ctcaccgaag tagcgagtgc gcgtgaggag ccgcatcacg
     1261 gggtgggtga aggccatgac cacgagcagg tcgaccacgg tggtgaggcc gagggtgaag
     1321 gcgaagccgc gcacgccgcc gacggcgagg aagtagagca cgaacgccgc caggaagttg
     1381 accccgtcgg aggcgaagat ggtgcgcttg gcccgcgccc agccgtactc gacggcgtcg
     1441 ctgaggccgc ggccctcgcg gacctcgtcg cggatgcgct cgaagtagac gatgaaggag
     1501 tccgccgtga tgccgatcgc gacgatcagg ccggcgacgc ccgcgaggga gagccggtaa
     1561 cccgttgtcc agccgagcag ggtgatcgtc aggtaggtga tgaaggccgc gacgccgagg
     1621 ctcgccgccg ccacggccgc gagggcgtgg tactgcagca gcatgtagat cagcacgagc
     1681 acgagtccga tcaggccggc gaggatcccg cgctgcagct gctcgagacc gagggtggcc
     1741 gagatctgct cctcgctctg cacctcgaag tcgaggggca gggacccgaa gttgagctgg
     1801 ttggcgagcg tggccgccga ctccctggtg aacgtgccgg agatcatggc ctcgccgttg
     1861 gcgatcgggt tgctcaccga gggggccgag acgaccaggc cgtccaggac gatcgcgaac
     1921 tggtcctgcg gggacggcag cgacatgagc cgggtggtgg cggtcgcgaa ggcttgctta
     1981 ccctcggcgt cgaagctcat cgtcacgatc cagttgttgg ttgtggcgcc cgtggtggtg
     2041 gtctccagcc ccgaggccgc cttcgtgatc tgcgttccct cgacgtcggc cgggccgagg
     2101 acgtacttcg cgctgccgtt ctgggcacag gcgatcacag cctccgccgg ggaggccgcg
     2161 tcgccccccg agaggttggc cgggtcggtg cagtccagca cgtagtagtc gaa
//
LOCUS       DASRKH010000246         2553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_18095, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000246 DASRKH010000000
VERSION     DASRKH010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18095"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            476..832
                     /locus_tag="VFC06_04015"
     CDS             476..832
                     /locus_tag="VFC06_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81090.1"
                     /translation="MNTLITRSALSGSLSFGLVPGIAWSAQGATYWISPYLTSTTYDY
                     TPSNWVMFTQGRGPTAIDAHEHAGDRRGSIGVRAVDSGGSTTQITWDWNREDEVHLYR
                     SQGVAKYKICKSVGDL"
     gene            842..1471
                     /locus_tag="VFC06_04020"
     CDS             842..1471
                     /locus_tag="VFC06_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81091.1"
                     /translation="MTDVAGRVGRAGGLRGLVVLMFVVVGGCSSSPSTPFEEAEPAFR
                     EHIEVAIEDARVGGASDAQLQILNEALDTEAISFEAAHSAAVAAVDCMNAAGLSARYK
                     EQSRPSGPATPGYVADIGLDDDGTNEAILSRCDAQEDFWVNLLYQTQPSSVALEDARI
                     ESLAPAFRECLESNGIDVDASATGSELQRQAIDVLVETEGAIDCLSVGN"
     gene            complement(1953..>2553)
                     /locus_tag="VFC06_04025"
     CDS             complement(1953..>2553)
                     /locus_tag="VFC06_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81092.1"
                     /translation="YQPYPFSNGRAGMAILSPAAAAAIGWPVVPTTLIVDAPKPIDST
                     QAAAVNRALQTVAGSGFLWLDVENGPRKQEALFTGALAFVAVALLIITATSIALALAR
                     SDARRDDFTLASLGAAPRTAKAITAWQGALIVGSATTIGLATALAWTWANNHALAQHG
                     YTTPWLWLIAGWVALPAITGALSWLLTRAARAIHYRLAA"
BASE COUNT          483 a          787 c          833 g          450 t
ORIGIN      
        1 gccagaaccg gccgcctcga cggcgacact cacctgttcc acgaaacttg ccacatcggc
       61 gcctccggtg ccggactgat ccccggcacc ggaggagcaa cctaatacga gcaggcttcc
      121 catcaagacg gtggccaaag agaaccgcct gaacgggcgg ctttgcggcg ataggcggtt
      181 caaatgccta gtggtcgccg tgatagacct tcatcgcagt cacggtccca tcgtaccggc
      241 cagtctgcga ggctgtgttg ccgccccagg agatgctcga aagatcccaa acccaactag
      301 gaagagcccg caatgcggcg ccgatctgcc aggcaccctt ccacgaggcg cccaggactt
      361 ttcacgggag tttcccatga tccccgtcgt cccgtcgccg cccgacgggc acagtgctag
      421 tttcgaagaa tacttctgga agtcgcaatg gagcggctga tcttgaggag gagtgatgaa
      481 tacactgatc accaggtcgg cattgtccgg ctcactctcg ttcggcttag ttcctggaat
      541 cgcctggagc gcgcagggag cgacgtactg gatttcccca tacctgacca gtaccacgta
      601 cgactacacc cctagcaatt gggtgatgtt tactcaaggc cggggaccta ccgccatcga
      661 cgcccacgag cacgcgggcg accgtagagg cagtatcggc gtacgcgctg tggactcagg
      721 tggctcgacc acgcagatta catgggactg gaatcgtgaa gacgaagtgc atctgtatcg
      781 ctcacaggga gtcgcgaagt acaagatctg caagagtgtc ggtgacttgt agttggcgac
      841 gatgaccgac gtggctggca gggtgggtcg cgcaggcggc ctccgtgggc tcgtcgtgct
      901 tatgttcgtc gttgtcggcg ggtgttcgtc gtcgccgtcg acaccgttcg aggaggcgga
      961 gcccgcgttc cgtgaacaca tcgaggtcgc tattgaggac gctcgcgtgg ggggcgcgag
     1021 tgacgcccaa ctccagatat tgaatgaggc actagacacc gaggcaatct cgttcgaggc
     1081 ggcgcactct gccgcggtcg cggcggttga ctgcatgaat gctgccggcc tcagcgcccg
     1141 gtacaaggaa cagagccgac catcgggccc ggcgacccct ggctacgtcg ctgacattgg
     1201 gttggacgac gacggaacca acgaggccat tctgagtcgt tgcgatgcgc aagaggactt
     1261 ctgggtgaac ctgctttacc agacgcagcc ttcctccgtg gccctggaag acgcgcgcat
     1321 cgagtcattg gctcctgcgt tccgggagtg cctcgagagc aacggcatag acgttgatgc
     1381 cagtgccaca gggagcgagt tgcagcgcca ggccatcgac gtgcttgttg agaccgaagg
     1441 cgccatcgac tgcctcagcg tcggcaacta gtcccgcgtc ggctgtcgtc cagccttgcg
     1501 tggtcgcact tcggcgctgt ggcggtcggc caaggtccgc acccacccca cactcaacta
     1561 ggaagagccg gcaattcgcg cacgacatgc ggcgtgacag aatcttcagc cgcgtcgagc
     1621 cgtcgattcg ccgacatcgt gatcgggcgc cccatagtca aacctcctgg caacgaaggt
     1681 atccaggagg gcagacaacc agccggcgct gctcgccgcg ggaggtacgt agcgaccctc
     1741 cgagacggcc acacatcgac cgcgacgttg ttcagcgaag caactgcccc atctggacaa
     1801 tgtcccgccc cagcgacacc gtgtcgcggg gcacgctggc tcgccaccca catccgacat
     1861 cgagataact cacatggagg gtggacagcg ccgcggtgag tgcggggcgt ttacccgacg
     1921 atcgcggtag tcacgcgaag tgaaatatcc cgtcaggcag cgagcctgta gtggatggct
     1981 cgtgccgccc gcgtgagtag ccacgagagc gcccctgtga ttgcggggag tgcgacccat
     2041 cctgcaatca gccatagcca cggcgtggtg tagccgtgct gtgcgagggc gtggttgttg
     2101 gcccaggtcc atgcaagagc ggtggcaagt ccgatggtgg ttgccgagcc cacgatgagt
     2161 gctccttgcc acgcggtgat cgcctttgcc gtgcgtggcg cggccccaag cgatgcgaga
     2221 gtgaagtcgt cgcggcgcgc gtcggaccgc gcgagcgcca gtgcgatcga ggtcgcggtg
     2281 atgatgagta gcgccacggc gacgaatgcc aatgctccgg tgaagagggc ctcttgtttg
     2341 cgtggaccgt tctcgacgtc aagccacagg aacccggagc ccgcgaccgt ttggagtgca
     2401 cggttgactg ccgccgcctg tgttgagtcg atcggcttcg gcgcgtccac aatgagcgtg
     2461 gtgggcacca cgggccagcc gatggccgct gccgcagcag gtgagaggat cgccatcccg
     2521 gcgcgcccgt tgctgaaggg gtacggctgg tac
//
LOCUS       DASRKH010000247         2094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_54193, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000247 DASRKH010000000
VERSION     DASRKH010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54193"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..931
                     /locus_tag="VFC06_04030"
     CDS             173..931
                     /locus_tag="VFC06_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein N(5)-glutamine
                     methyltransferase"
                     /protein_id="HZL81093.1"
                     /translation="MSDLVAQLREAGCVFAEEEAAILFAAASDATELAALVARRIAGE
                     PLEQVVGWAEFAGMRIPVTPGVFVPRRRTELVARIAERVAPASGTVVDLCCGAGAIGI
                     ALATWREDLTVIVADIDPGAVDLARRALSPLGGTAVASDMDASLPPELMGSVDVVASC
                     PPYVPTRQIALMPHEARDHEPRKALDGGKDGARMQARVFQAAARLLVPGGVCVVETSD
                     HVVDATVAAATAAGFAATVEEDDVLGAVVVVATA"
     gene            979..1140
                     /locus_tag="VFC06_04035"
     CDS             979..1140
                     /locus_tag="VFC06_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81094.1"
                     /translation="MALFMPMLRESAEMMYEFDEPFVVSSAEFERVFGWGATSWDDAV
                     AQMGAAVRA"
     gene            1150..>2094
                     /locus_tag="VFC06_04040"
     CDS             1150..>2094
                     /locus_tag="VFC06_04040"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HZL81095.1"
                     /translation="MSTLTRADVDRVRLGSLLIAGPADPGRTVLSIVEHFLAMQAQDA
                     PGVAWSLALRNGGTESQTAHALAERDVVRTWPMRGTLHLIPSRDAKWMLDLMGGKALA
                     GAARRRQVLDLPENVAYRAVDILAARVAEAEGPVARKDCLMALADAGIALENQHGYHL
                     LWFASQLGQLAGGPPQGKEQTCVSLDTWAPLHRAPSREEALALVAAAYVRGHGPITER
                     DLMRWTGLGLHECRVGLTTAEGILSVDTGAGPAWVTGFALEAPAIAPTRLLPGFDEFM
                     LGYAADGRAIDPAHHRAVVPGNNGMFKPTVVDCGRVVAL"
BASE COUNT          290 a          695 c          769 g          340 t
ORIGIN      
        1 gtgttggtgc gcagccgctc ggtcatgagg cgcatcgact cggcggcttc cggcgtgccc
       61 cactcgccgc gggtcccggg gaagccccac gtcatcccac gtacatccat tttcggtcgg
      121 gaagtcgtca tggcctcacg ctaacgcgac gctcggaagg tagcgtcgtc gtatgagcga
      181 tctggtcgcg cagctgcgcg aggccggctg cgtgttcgca gaggaagagg ccgccatcct
      241 gtttgcggcc gcgtccgacg ccaccgaact cgcggccctg gtcgcgcgcc ggatcgccgg
      301 cgagccgctc gagcaggtcg tggggtgggc cgaattcgcg ggcatgcgca tcccggtcac
      361 gcctggggtg tttgtgccgc ggcgacgcac cgaactggtg gcgcggatcg cggagcgcgt
      421 cgcgccagca tcgggcacgg tggtcgacct gtgttgcggc gcgggggcga tcgggattgc
      481 tctcgcgacg tggcgcgaag acctcacggt gatcgttgcc gacatcgacc ccggggcggt
      541 agacctcgcg cggcgcgcgc tctcgccgct gggcggcacc gcggtggcgt cggacatgga
      601 cgccagcctg ccgcctgagc tcatgggctc tgtcgatgtg gtggcgtcgt gtccgcccta
      661 cgtccccacc cgtcagatcg cgttgatgcc gcacgaggcg cgggaccacg agccccgcaa
      721 ggccctggac ggcggcaagg acggagcccg catgcaggcg cgagtgtttc aggccgccgc
      781 ccgactgctc gtgccgggcg gggtgtgcgt ggtcgagacc agcgatcacg tggtcgacgc
      841 gaccgtcgcc gccgccacgg cagccgggtt cgccgcgacc gtcgaggagg acgacgtgct
      901 gggcgcggtg gtggtcgtgg cgacggcgta atcgcatccg ccgtgtgcgc cgagaggcga
      961 ggccttgccg tgcgcggcat ggcactcttc atgccgatgc tgcgcgagag cgcggagatg
     1021 atgtacgagt tcgacgaacc gttcgtcgtc tcgtccgcag agtttgagcg ggtctttggc
     1081 tggggagcaa cttcgtggga cgacgctgtg gctcagatgg gagcggcggt acgggcgtag
     1141 cgtaggaata tgtcaacgct gacgcgggcc gacgtcgatc gtgttcgcct cggctcgctc
     1201 ctgatcgccg gccccgcgga cccgggccgc accgtgctca gcatcgtcga gcatttcttg
     1261 gcgatgcagg cgcaagacgc ccccggcgtg gcctggtcgc tcgcccttcg caacggaggc
     1321 accgagtcac aaactgccca cgcactggcc gaacgcgacg tagtccgcac ctggccgatg
     1381 cgcggcaccc ttcacctcat tccgtcgcgc gacgccaagt ggatgctcga cctcatgggc
     1441 ggcaaggcgc tcgcgggcgc ggcgcggcgg cggcaagtgc tggacctgcc ggagaacgtc
     1501 gcctaccgcg cggtcgacat cctcgccgct cgggtggccg aggccgaggg cccggtcgcg
     1561 cgcaaggact gcctgatggc gcttgccgat gccggcatcg cgcttgagaa tcagcacggc
     1621 tatcacttgc tgtggtttgc ctcgcaactg ggtcaattgg ctggcggccc tcctcagggc
     1681 aaagagcaga cgtgtgtcag cctggacacg tgggcgcctc tgcatcgtgc cccgtcgcgg
     1741 gaagaggccc ttgcgctggt agcggccgct tatgtgcgcg gccacggacc gatcacggaa
     1801 cgcgacctga tgcgctggac cgggctggga ttgcacgagt gtcgcgtcgg actcacgacc
     1861 gcggagggca tcctctcggt ggacaccgga gcgggcccgg cgtgggtcac cgggttcgcg
     1921 ctggaggccc ctgcgatcgc gcccacgcga ctgcttccag ggttcgacga gttcatgctc
     1981 ggctacgcgg ccgacgggcg cgccatcgac cctgcccacc acagagcggt ggtgccgggc
     2041 aacaacggaa tgttcaagcc cacggtcgtg gactgcggtc gtgtggtcgc actg
//
LOCUS       DASRKH010000248         2290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_5827, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000248 DASRKH010000000
VERSION     DASRKH010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5827"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..953
                     /locus_tag="VFC06_04045"
     CDS             6..953
                     /locus_tag="VFC06_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZL81096.1"
                     /translation="MTAVLECAHLVRTFGDLRAVEDVSLTIAPGETYGLLGPNGAGKT
                     TTISMICGLLAPTAGTVRVLGREITPAASDAKAAIGLVPQDIALYPDLSARENLRFFG
                     RLQGLRGAALEARIDETLDVVGLRDRAKDRIDTYSGGMKRRANIAVGMLHAPTLLVLD
                     EPTVGVDPQSRNSILEAIANLGTAGMAVLYTTHYMEEAERLCDRIGIIDVGRLIAEGT
                     RAELVAGLGSASRVTVRAEGTAPALEAWRAALATLPGVTVSATATGADLACADSTATV
                     AAAFQRAGAHGVAISGVEVAAPDLESVFLHLTGKGLRDE"
     gene            953..2155
                     /locus_tag="VFC06_04050"
     CDS             953..2155
                     /locus_tag="VFC06_04050"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZL81097.1"
                     /translation="MAAPNVLTQALLVARLDLRRRLRDRSVLIQVILAPVLLALIIGG
                     AFSGDSGGLRADIWLADADGSELSTQIVAALAGQSEPGGEGVSFVAKPGLDAAAAEAA
                     VDADEADAVVVIPAGFGAAVRAGEAAELRVLGDAGDDVVAGIARSVADGIANGVQTQR
                     AAIATTLTAAAQLGEEVSADDVARVATATPAAITVRDSRFENTFSMMSFFAPGMAMIF
                     LFFVMGAAARSLVTERREGTLPRMLAGPTSPTAVLLGKALAVLTLGFTSLLMVWGITS
                     LGFGVDWGDPLGVLLVITGAVVAIAGISLVITGLARTESQSEALTIVFALVLSVLGGS
                     FVYTATGFLAEVRKFTPNGQALMAFSDLAAGNASPADVLPAVLLLLGGGLVTGAIGLL
                     AIRRGLAP"
BASE COUNT          291 a          867 c          799 g          333 t
ORIGIN      
        1 ggtacatgac cgccgtgctc gaatgcgctc acctcgtccg gacgttcggg gacctgaggg
       61 cggtggagga cgtcagcctc accatcgcgc ccggggagac ctacggcctg ctcggcccga
      121 acggcgcggg caagaccacc accatctcga tgatctgcgg gctgctggcc cccaccgccg
      181 gcacagtgcg cgtgctgggc cgcgagatca ccccggcggc gtccgatgcc aaggcagcga
      241 tcggtctggt gccgcaggac atcgcgctct acccggacct ctcggcccgg gagaacctcc
      301 gcttcttcgg ccggctgcag ggcctgcgcg gcgccgcgct cgaggcccgc atcgacgaga
      361 cgctcgacgt cgtcggcctc cgcgaccggg cgaaggaccg catcgacacc tactccggtg
      421 gcatgaagcg ccgcgccaac atcgcggtgg ggatgctgca cgcccccacg ctgctggtgc
      481 tggacgaacc cacggtgggc gtggacccgc agagccgcaa ctccatcctc gaggcgatcg
      541 cgaacctcgg gaccgcgggc atggccgtgc tctacaccac ccactacatg gaggaggccg
      601 agcggctctg cgaccgcatc ggcatcatcg acgtcggccg cctgatcgcc gagggcaccc
      661 gggccgagct cgtcgcgggc ctcggctccg ccagccgggt gaccgtccgg gccgaaggca
      721 ccgcccccgc actggaggcg tggcgggccg cgctcgccac actccccggg gtcaccgtca
      781 gcgccaccgc gaccggcgcg gacctcgcct gcgccgactc cacggccacc gtcgcggcgg
      841 ccttccagcg cgccggggcg cacggggtgg cgatcagcgg ggtcgaggtg gcggccccgg
      901 acctggagtc ggtgttcctg cacctcactg gcaagggcct ccgggacgag tgatggccgc
      961 cccgaacgtc ctcacccaag cgctgctcgt cgcccgcctg gacctcaggc ggcgcctgcg
     1021 cgaccgctcc gtgctcatcc aggtgatcct cgcccccgtg ctcctcgccc tcatcatcgg
     1081 cggcgcgttc agtggcgaca gtggcggcct gcgggccgac atctggctgg ccgacgccga
     1141 cggctccgaa ctctccacgc agatcgtggc cgctctggcc ggccagtccg agcccggtgg
     1201 tgagggcgtc tcgttcgtcg cgaagcccgg cctggacgcc gccgcggcgg aagcggccgt
     1261 cgacgcggac gaggcggacg ccgtcgtcgt gatccccgcg ggcttcggcg ccgccgtcag
     1321 ggccggcgag gcggccgagc tcagggtgct cggcgacgcg ggtgacgacg tcgtcgcggg
     1381 catcgcgcgg agcgtcgccg acggcatcgc gaacggggtc cagacccagc gcgccgccat
     1441 cgccaccacg ctcaccgcgg ccgcgcagct gggcgaggag gtgtccgccg acgacgtagc
     1501 gcgcgtggcc accgccaccc cggcggcgat cacggtgcgg gactcccggt tcgagaacac
     1561 cttcagcatg atgtcgttct tcgcgccggg catggcgatg atcttcctct tcttcgtgat
     1621 gggcgccgcc gcgcgcagcc tcgtcaccga gcgacgcgag ggcacgctcc cccggatgct
     1681 cgccgggcct accagcccga ccgcggtcct gctcggcaag gccctcgcgg tgctcaccct
     1741 gggcttcacg agcctcctga tggtctgggg gatcacgtcg ctggggttcg gcgtggactg
     1801 gggtgacccg ctcggtgtgc tgctggtgat caccggcgcg gtggtggcga ttgccggcat
     1861 ctcgctggtc atcaccggcc tcgcccgcac cgagtcccag tcggaggcgc tcaccatcgt
     1921 gttcgcgctc gtgctgagcg tgctcggcgg gtccttcgtg tacaccgcga ccgggttcct
     1981 cgccgaggtt cggaagttca cccccaacgg gcaggcgctg atggcgttct cggaccttgc
     2041 cgccgggaac gcgagcccgg cggacgtgct ccccgccgtc ctcctcctcc tgggcggcgg
     2101 cctggtcacg ggagcgatcg gcctgctggc gatccgcagg gggctggctc cgtgaacgcc
     2161 gcccttgcca tcgcggtcct cgagatccgg cgcttcctgc gggaacgcat cacgctgttc
     2221 accaccatcc tgatgccgac ggcgctcatg ctcatcatcg gcaccgcctt cgggtcaggc
     2281 acgtccgaac
//
LOCUS       DASRKH010000249         2033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_17464, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000249 DASRKH010000000
VERSION     DASRKH010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17464"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="VFC06_04055"
     CDS             <1..634
                     /locus_tag="VFC06_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015881763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HZL81098.1"
                     /translation="WVVHPGALSLGDVRVEPDLSNAGPFMAAALAAGGTVRIPGWPAV
                     TTQPGALYPELLERMGGRCVLDGDVMTVTGTGEVRGITADLSPAGEITPTIAALCAIA
                     AGPSRLTGIGHLRGHETDRLVAIAAEVQRVGGTCEAGDDSLTFGGVPSPDDASGGLSG
                     AVLETYHDHRMATFAAVVGLVVPGIQVINVGTTAKTMPDFPRMWADMLES"
     gene            complement(631..1614)
                     /locus_tag="VFC06_04060"
     CDS             complement(631..1614)
                     /locus_tag="VFC06_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZL81099.1"
                     /translation="MTDALRIAVVGAGGWGAQHARIVSRRRDTELVGIVGRDPDRTAA
                     RAATFDTAPFTDLDTMLDEARPDLVTVCIPNEAHFEPTMHLLQRGMPLLVEKPLVFDL
                     AEADALLAEADRQGLFFAINLNHRYAEPVMRAKAAIDAGELGDVVFATWRFGGEPNFG
                     DSPHANLIETQVHGLDMLEQLCGPISSVAAQMTDMTHPGTYTTLAVALQFASGAVGSF
                     VGSYDSSYAYPDAHVVEVNGTSGRLIIEDTVKRLTVSAAGDETRKVWEPGYFNDEART
                     FEYTFDRHMDALLAAFRAGGLPPVHAREGRRALALAMGIIASFEKGVRVAV"
     gene            1708..>2033
                     /locus_tag="VFC06_04065"
     CDS             1708..>2033
                     /locus_tag="VFC06_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HZL81100.1"
                     /translation="MTDWSHYDESDAKVRPSRSSRPRSKRRPAHESAVEGTVIGVDRG
                     RYTVQIGEAAVMAVKAREVGRRGLVIGDVVAVVGDTSGTPDTLARVVRREERRNSLRR
                     TADDDAV"
BASE COUNT          321 a          692 c          700 g          320 t
ORIGIN      
        1 gtgggtggtg cacccaggag ccctgtcgct gggcgacgtg cgcgtggagc ccgacctgtc
       61 caacgccgga cccttcatgg ctgcggcgct cgcggcgggc ggcacggtgc gaatccccgg
      121 ctggcccgca gtcaccacgc aacccggcgc gctctacccg gagttgctgg agcgcatggg
      181 cgggcggtgc gtgttggacg gcgacgtcat gaccgtgacc ggcacgggtg aggtgcgcgg
      241 catcaccgcc gacctgtccc ccgccggcga gatcaccccc acgattgcgg cgctgtgcgc
      301 gatcgctgca gggccctcca ggctcaccgg catcgggcac ctgcgcgggc acgagactga
      361 ccggttggtc gcgatcgccg ccgaggtgca gcgggtcggc ggcacgtgcg aggcaggcga
      421 cgattccctg acgtttggcg gagtgccctc cccggacgat gcttctggcg ggctgtcggg
      481 cgccgttctg gagacctatc acgaccaccg gatggccacc ttcgcagccg tcgtcggcct
      541 cgttgtccct ggtattcagg tcattaacgt gggcacgacg gccaagacga tgccggactt
      601 cccgcgcatg tgggctgaca tgctggaaag ctagaccgcg acccgcacgc ccttctcaaa
      661 cgatgcgatg atgcccatcg cgagcgcaag cgcgcgtcgt ccctccctcg cgtgaaccgg
      721 gggcagcccg cccgcgcgga acgcagccaa cagggcatcc atgtgccggt cgaaggtgta
      781 ttcgaaggtg cgagcctcgt cgttgaagta cccgggctcc cacaccttgc gcgtctcgtc
      841 gcccgcggcg gacacggtca atcgcttcac ggtgtcctcg atgatgagcc tgccgctggt
      901 gccattgacc tcgaccacgt gggcgtcggg gtaggcgtag gacgagtcgt aggacccgac
      961 aaacgagccc accgcacccg aggcgaattg cagggctacc gcgagcgtcg tgtacgtccc
     1021 ggggtgcgtc atgtcggtca tctgcgccgc gacggaactg atcgggccgc acagctgctc
     1081 aagcatgtcg aggccgtgca cctgcgtctc gatcaggttg gcgtgggggc tgtcgccaaa
     1141 gttgggctcg ccgccaaagc gccacgtggc gaacacgacg tctccgagct cgcccgcgtc
     1201 gatcgccgcc ttggctcgca tcacgggctc cgcgtagcgg tggttgaggt tgatcgcgaa
     1261 gaacaggcct tggcgatcgg cctcggcgag tagcgcatcc gcctccgcaa ggtcgaatac
     1321 cagtggcttc tctaccaaga gcggcatgcc gcgctggagg aggtgcatcg tcggctcgaa
     1381 gtgagcctcg ttgggaatgc acaccgtaac gaggtcgggg cgagcctcgt cgagcatcgt
     1441 gtcgaggtcg gtgaacgggg ccgtatcgaa ggtggcggcg cgagcggccg tgcggtccgg
     1501 atcgcgcccc acgatcccca ccaactcggt gtcacgtcgt cgcgacacga tgcgcgcgtg
     1561 ctgggcaccc caacctccgg cgcccaccac cgcaatcctc agtgcatcgg tcatcgtcga
     1621 atcctccatc gcattcgcag gaacgctact cacactaacc gcctgggctc gctagtctcg
     1681 gagcatgacg acatcaccgg cggccgcgtg acggactgga gccactacga cgagtccgac
     1741 gccaaggtgc ggccctcgcg tagttcgcga cctcgctcca agcgccgccc cgcccacgaa
     1801 agcgccgtcg agggcacggt gatcggcgtc gaccgcggtc gctacaccgt ccagatcggc
     1861 gaggccgccg tcatggccgt caaggctcgc gaggtgggac ggcgcggact ggtgatcggc
     1921 gacgtggtgg cggtcgtcgg cgatacctca ggcacgcctg acaccctggc gcgggttgtc
     1981 cggcgcgagg agcgcagaaa ctccctacgg cgcacggcgg atgacgacgc tgt
//
LOCUS       DASRKH010000250         2079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_31334, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000250 DASRKH010000000
VERSION     DASRKH010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31334"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..1389)
                     /gene="tyrS"
                     /locus_tag="VFC06_04070"
     CDS             complement(124..1389)
                     /gene="tyrS"
                     /locus_tag="VFC06_04070"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HZL81101.1"
                     /translation="MTEHILDDLAWRGLISQTTGDDELRAAIDAGPLTLYCGFDPTAP
                     SLHIGNLVQILTQRRFQNAGHHSLMLVGGATGLIGDPKMAGERTLNPVDVVHEWVERI
                     RQQIEPFMDFEGDNPARMVNNYDWTKDLSAIELLRDIGRHYRLGTMIAKDTVARRLNS
                     DEGISYTEFSYQLLQGMDYLELYRRYGCTLQTGGSDQWGNLTSGTELTRKAEGVSVHA
                     LATPLITKADGTKFGKTESGTVWLDPALTSPYAFYQFWLNQSDADVVGYLKVFTFLSR
                     EQIEELEAKVEAEPFRREAQRTLAWEVTCLVHGEVAAQGAVDASRALFGQGELTALDA
                     GTLDGCAQELGGASVAGGTLVVDALVEAGVTDSKSAARRAIQEGGAYVNNVKVADADA
                     ALDPADALAGGWFVLRRGKKTIGLVRTSP"
     gene            complement(1386..>2079)
                     /locus_tag="VFC06_04075"
     CDS             complement(1386..>2079)
                     /locus_tag="VFC06_04075"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZL81102.1"
                     /translation="WTLLRPDPLVLARTREHDASMHSVQPDPTPAEARARSRGIATGA
                     LVMIVSQMVMIAVMTMTPIHMRSHGHDVSAVGFVIGVHVAAMYLPSPLSGWLVDRWGA
                     GTVAMLSAVTFVGAGAVGAWSPPASIPALTLSLALLGLGWSFGLNAGTAMVTTAAPLA
                     NRARIQGNVDFLIALAGATGGALSGVVVAGAGFATLALGGGAVALLLVPVLAVTRAAH
                     PRQRAAGTMAAS"
BASE COUNT          356 a          707 c          666 g          350 t
ORIGIN      
        1 gtgttggcaa caggtgtgac attacggcct gatcacaccg caagtcaaat cgcttgcatc
       61 tcgggcgtgt cgtgggcatc gttcctggta cgtggcctgt tgcgccgacg cgggtgcgac
      121 ccgctaaggg gacgttcgca caagtccgat cgtctttttt ccgcgcctga gcacgaacca
      181 tccgcccgcc aaggcgtccg cgggatcgag cgccgcatcg gcgtcggcaa ccttcacgtt
      241 gttgacgtag gcaccgccct cttggatcgc ccttctggcg gccgatttcg agtccgtgac
      301 ccccgcctcc acgagcgcgt cgaccaccag cgttcctccg gccacagacg cgccccccag
      361 ctcctgcgcg caaccgtcta gcgtgccggc gtcgagtgcc gtcaactcac cttggccgaa
      421 cagtgcccgc gacgcatcga cggcgccctg agcggcgacc tcgccgtgca cgaggcacgt
      481 cacctcccac gcgagggtgc gctgcgcctc ccggcggaac ggctccgcct ccaccttcgc
      541 ctccagctcc tcgatctgct ccctgctcag gaaggtgaag accttgaggt accccacgac
      601 gtcggcatcg gactgattga gccagaactg gtaaaacgcg tacggacttg tgagcgcagg
      661 atcgagccac accgtccccg actccgtctt gccgaacttg gttccgtcgg ccttggtaat
      721 gagaggggtc gccagggcat gcacggagac gccctcggcc ttgcgcgtga gttccgtgcc
      781 cgacgtcagg tttccccact ggtcggagcc gcccgtctgg agcgtgcagc cgtagcgtcg
      841 gtagagctcg aggtagtcca tcccctggag aagttggtac gagaactccg tgtaggagat
      901 gccctcatcg gagttgaggc gacgcgcgac ggtatccttc gcgatcatcg tgcccaggcg
      961 gtaatggcgg ccgatgtcgc gcagcaattc aatcgcgctg aggtccttgg tccagtcgta
     1021 gttgttgacc atgcgcgcgg gattgtcgcc ctcgaagtcc atgaacggct cgatctgctg
     1081 cctgatccgc tcgacccact cgtgcaccac gtcgacggga ttgagcgtgc gctcgccagc
     1141 cattttggga tcgccgataa gtccggtcgc gcctcccacc agcatgagcg agtggtggcc
     1201 cgcgttctgg aaacgccgtt gcgtcaaaat ctgcaccagg ttgccgatgt gcaggcttgg
     1261 tgcggtggga tcgaacccgc aatacagcgt gaggggtccc gcatcgatcg cggcgcgcag
     1321 ttcatcgtcg ccggtagttt gtgagatcag cccgcgccac gcgaggtcgt ccaggatgtg
     1381 ttcagtcatg atgcggccat tgtgccagca gcgcgctggc gcggatgagc cgcacgcgtc
     1441 acggccagca cgggcaccag caacagtgcc accgcgccgc cgccgagcgc gagcgttgcg
     1501 aagcctgctc ccgccaccac aacaccggag agggcgccgc cggtcgcgcc agcgagggcg
     1561 atgaggaagt cgacgttgcc ctggattcgc gctcggtttg cgagaggcgc cgcggtggtg
     1621 accatggcag tacccgcgtt caacccgaac gaccacccaa ggcccaagag cgcgagggag
     1681 agcgtcagcg ccggaatcga cgcgggtggg ctccatgccc cgaccgcacc cgcgcccacg
     1741 aaggtgacgg cggacagcat ggccaccgtg ccagcccccc agcgatccac gagccatccg
     1801 gacaggggtg aaggcaggta catcgccgcc acgtgaacgc cgatgacgaa ccccaccgcg
     1861 ctgacgtcgt ggccgtggct ccgcatgtga atgggcgtca tggtcatcac cgcgatcatg
     1921 accatctgcg acacaatcat gaccaacgcg ccggtggcga tacccctgct cctcgcacgg
     1981 gcctcggcag gcgtcggatc gggctgaaca gagtgcatcg acgcatcgtg ttcgcgggtg
     2041 cgggccagca cgaggggatc cggcctcaac agggtccac
//
LOCUS       DASRKH010000251         2665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_8915, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000251 DASRKH010000000
VERSION     DASRKH010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8915"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VFC06_04080"
     CDS             <1..690
                     /locus_tag="VFC06_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HZL81103.1"
                     /translation="APVIIVLAVISILYGGLVALAQRDFMRLTAMTSVSHFGFIVLGI
                     FVGSATAMTGSMIYMVAHGLSTAAMFLVLGFLERRGRSQWIPAYRGMQRITPVLAGLF
                     LVAGLASIALPGLSGFVPEYLVLLGTFKANTLAAVFAALGVIIAALYVLLPYQRMFTG
                     PKDEGITGAADLDGREKLAMAPLVIAMLALGIFPGPVLDVLSPVADQTAPLIQSAQGV
                     DTAMAIEGSAK"
     gene            687..2252
                     /gene="nuoN"
                     /locus_tag="VFC06_04085"
     CDS             687..2252
                     /gene="nuoN"
                     /locus_tag="VFC06_04085"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012877265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HZL81104.1"
                     /translation="MNQFVAPAIEWAALTPLLIIFGAAVVGVLLEAFLPRSARRPVQV
                     GLALVAIASALVAVVWRWTVLQDGGPREVVAGAVIEDGPALAAQAILLVVAFIAMLVI
                     ADRTETGEGAFVASGATRPGSEEEERATNAGLVQGEVFPLTLFSLLGMMVFCSAGDLL
                     TLFVALEVLSLPLYILSGLARRRRLLSQEASLKYFLLGALSSALFLFGIALLYGFSGS
                     FRLSLIATAVPATAGMDSLLLAGSMLVLIGLLFKVGAVPFHAWTPDVYQGAPTPVTGF
                     MAAATKLAAFAAMMRIMYVVLPGLKWDLAPVLWTIIGLTMLLGTVLGIVQGNVKRMLA
                     YSSIAHAGFVLLGVVALQQSGISSVLFYLLAYGLATVGAFAVITLVRERDPEGLVTAE
                     ANELEQWAGLGNRDPWLAGAMLVFLLSFAGIPLTAGFMGKFTVFTAAVQGGLGPLVVL
                     AVLCSAATAFFYVRLVVLMYFTAPDGKSTVTVESEGMTTVAVAICAIGTVALGVFPGP
                     VLDMLAGAATYLP"
     gene            2306..>2665
                     /locus_tag="VFC06_04090"
     CDS             2306..>2665
                     /locus_tag="VFC06_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005269138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HZL81105.1"
                     /translation="MAVVEQQLARATAHAEHSIAEAASHLAKAGGKRLRPMLTLLGSQ
                     LGSGVNDEVLRAAVAVELTHLATLYHDDVMDSATLRRGAPAAQEVWGNTVAILTGDLL
                     FARASQIVASLGADAVLI"
BASE COUNT          316 a          960 c          890 g          499 t
ORIGIN      
        1 gctccggtga tcatcgtgct ggccgtcatc tcgatcctct acggcggcct ggtggcgctc
       61 gcacagcgcg acttcatgcg gctgaccgcc atgacctcgg tatcgcactt cggcttcatc
      121 gtcctgggca tcttcgtggg gtccgccacc gcaatgaccg gttcgatgat ctacatggtg
      181 gcgcacgggc tctcaacggc cgccatgttc cttgtcctcg ggttcctcga gaggcggggc
      241 cgcagccagt ggatcccggc ctaccgcggg atgcagcgca tcacgccggt cctggccggc
      301 ctgttcctcg tggccggcct cgcgtccatc gcactgccgg gcctgtccgg tttcgttccc
      361 gagtacctcg tgctgctcgg caccttcaag gccaacaccc tcgcggcggt cttcgccgcc
      421 cttggcgtca tcatcgcggc cctctatgtg ctgctgccct accagcgcat gttcaccgga
      481 cccaaggatg agggcatcac gggcgccgcg gacctcgatg gcagggagaa gctggcgatg
      541 gcgccactcg tcatcgcgat gctggcactc ggtatcttcc ccggacccgt cctcgacgtg
      601 ctctcgcccg tcgccgacca gaccgcgccg ctcatccaga gcgcgcaggg cgtcgatacc
      661 gccatggcca ttgaagggag cgcgaagtga accagttcgt ggcaccggcg attgagtggg
      721 cggcactgac ccccctgctg atcatcttcg gcgcggccgt ggtgggtgtg ctgctcgagg
      781 ccttcctccc gcgttccgcg cgccggcccg tccaggtggg gctcgccctc gtcgccatcg
      841 cgagcgcact cgtcgccgtg gtgtggcgct ggaccgtgct gcaggacggc gggccacgcg
      901 aggtcgtggc cggcgccgtg atcgaggacg gccccgcgct ggcggcgcag gcgatcctgc
      961 tcgtcgtcgc gttcatcgcg atgctggtca tcgcggaccg caccgagacc ggtgagggcg
     1021 cgttcgtggc gtcgggcgcc acccgtcccg gcagcgagga ggaggagcgt gccacgaatg
     1081 cgggcctcgt ccagggcgag gtgttcccgc tgacgctttt ctccctgctg ggcatgatgg
     1141 tgttctgctc ggccggggac ctgctcacgc tgttcgtggc cctcgaggtg ctgtcgctgc
     1201 cgctgtacat cctctccggg ctcgcacgcc ggcggcggct cctcagccag gaggcctccc
     1261 tcaagtactt cctgctcggc gcgctctcgt ccgcgctgtt cctcttcggt atcgcgctgc
     1321 tgtacggctt ctccggttcg ttccggctct cgttgatcgc caccgccgtc ccggccacgg
     1381 cgggcatgga ctcactcctg ctcgcgggct cgatgctcgt cctgatcggc ctgctcttca
     1441 aggtgggcgc cgtcccgttc cacgcctgga caccggacgt gtaccagggc gcccccacac
     1501 cggtgaccgg cttcatggca gcggcaacga agctcgcggc cttcgccgcg atgatgcgca
     1561 tcatgtacgt ggtgctcccc ggcctgaagt gggacctcgc gccggtgttg tggacgatca
     1621 tcgggctgac catgctgctc ggcacggtcc tcggtatcgt ccagggcaac gtgaagcgca
     1681 tgctggcgta ctcctcgatc gcgcacgcgg ggttcgtcct cctcggcgtg gtggcgctgc
     1741 agcagagcgg gatctcgtcc gtgctcttct acctgctcgc gtacggcctc gccacggtcg
     1801 gtgcgttcgc cgtcatcacc ctggtgcgtg agcgggaccc cgagggcctc gtcaccgcgg
     1861 aggcgaacga gctcgagcag tgggccgggc tcggcaatcg cgacccatgg ctcgccggcg
     1921 ccatgctggt gttcctgctc tccttcgcgg gaatcccgct cacggcgggc ttcatgggca
     1981 agttcaccgt cttcaccgcg gccgtccagg gcggcctcgg cccactcgtg gtcctcgcgg
     2041 tgctctgctc cgcggcgacc gcgttcttct acgtccgtct cgtcgtgctc atgtacttca
     2101 cggcgccgga cggcaagtcc accgtgaccg tggagtccga gggcatgacg accgtggccg
     2161 tggccatctg cgccatcggt acggtggcgc tcggtgtgtt ccccggtccg gtcctcgaca
     2221 tgcttgcggg cgccgccacc taccttccgt gagtgagtac gtgaatcctg catccccttc
     2281 cctgatcgac cgcctcgagc ccgagatggc cgtggtggag caacagctcg cccgggccac
     2341 cgcccacgcc gagcactcca tcgcggaggc ggcgtcgcac ctggcgaagg ccggcgggaa
     2401 gcgcctgcgg ccgatgctga ccctgctcgg gtcacagctc ggcagcgggg tgaacgacga
     2461 ggtgctgcgt gccgccgtcg ccgtcgagct gacgcacctg gccacgctct accacgacga
     2521 cgtcatggac tccgccacgc tgcgccgtgg cgcgccggcg gcgcaggagg tgtggggcaa
     2581 cacggtggcg atcctgaccg gggacctgct gttcgcgcgc gcctcccaga tcgtggcctc
     2641 gctcggggcc gacgcggtcc tcatc
//
LOCUS       DASRKH010000252         2100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_22561, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000252 DASRKH010000000
VERSION     DASRKH010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22561"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="VFC06_04095"
     CDS             complement(<1..852)
                     /locus_tag="VFC06_04095"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HZL81106.1"
                     /translation="MSTLPPSVRLETGQGGLPVVRVTAAAASAEIYLHGAHVTSWVPR
                     GQQPVVWLSAASRFEADAAIRGGVPICFPWFGARAGHPEAPMHGFARLSDWELVEAHD
                     EGDVVIVQLRLSDSEATRASAWPFMFEATFTVAVGAQLSITLDVANRDSAEFTFEEAL
                     HTYLAVGDIHATEVSGLEGTPYIDRVAGPDALPGESGPVRFAGLTDRIYLGTTARATV
                     RDPAGGRLIGISKDGSDTTVVWNPWIDNARAMADFGDDEWTGMVCVETCNVRDAHHPG
                     PLVVAEVG"
     gene            976..>2100
                     /locus_tag="VFC06_04100"
     CDS             976..>2100
                     /locus_tag="VFC06_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HZL81107.1"
                     /translation="MADDAIRRLLAAPPDLPVLAGLPALTAAVGARGLAVVQAPPGTG
                     KTTMVPPALAVLGAGRVVVTQPRRIAVRAAARRLAQLLGEPVGESVGYAVRGEHRSGR
                     ATRIEIVTTGVLLRRLQRDPDLPGVDAVVLDECHERALDGDLALALLVDVRTHLRPDL
                     TLVAMSATVEAERTADLLGGTDPAPVVAVTSALHPVTQVWCPPPARVARTDERGVTPG
                     FLDHVAACARRALAECPGDVLVFVPGAGEVNGVARRLAGIDADLRPLHGRLSHRDQDL
                     ALTPGPRRRVVVSTAVAESSLTVPGVRVVVDAGFSREPRTDHRRGLAGLVTVGVSRAA
                     ADQRAGRAGREGPGAVYRCWSLAEHARLRQHPEPEIRTADL"
BASE COUNT          251 a          757 c          789 g          303 t
ORIGIN      
        1 gccgacttcg gcgacgacga gtggaccggg atggtgtgcg tcgcgcacgt tgcacgtctc
       61 gacgcacacc atcccggtcc actcgtcgtc gccgaagtcg gccatcgccc gggcgttgtc
      121 gatccacggg ttccagacga ccgtcgtgtc cgagccgtcc ttgctgatgc cgatcaagcg
      181 cccaccggcc gggtcccgca ccgtcgcccg cgcggtcgtc ccgaggtaga tgcggtcggt
      241 gaggccggcg aagcggaccg gtccggactc accggggagg gcatcggggc ctgcgacacg
      301 atcgatgtac ggcgttccct cgagaccgct gacctcggtg gcgtggatgt cgccgacggc
      361 gaggtaggtg tgcagagcct cctcgaacgt gaactccgcg gagtcgcggt tcgcgacgtc
      421 cagggttatc gacagctgag cgccgaccgc gaccgtgaac gtcgcctcga acatgaacgg
      481 ccaggcggac gcccgggtcg cctcgctgtc cgagagccgg agctgcacga tgacgacgtc
      541 gccctcgtcg tgcgcctcga cgagctccca gtcggacagc cgggcgaagc cgtgcatcgg
      601 ggcctcgggg tgaccggcgc gcgcgccgaa ccacgggaag cagatcggca cgccgccgcg
      661 gatcgcagcg tcagcttcga accggctcgc cgcgctcagc cacaccacgg gctgctgccc
      721 gcgtgggacc cagctcgtca cgtgagcacc gtgcaggtag atctccgcgc tcgctgcggc
      781 ggcggtcacg cgcacgacgg gcagtccgcc ctgaccggtc tcgaggcgga cgctgggggg
      841 cagggtcgac atgtcggctc cgatggtcgt ccggtctgcg atcaccctgg atcgggtgcc
      901 ggctccaggg tcctcttgcg acggtctcga cgcacgtcgt cgctcggacc tcggtgaggc
      961 gggcacactt cacgtgtggc cgacgacgcg atccgcaggc tgctggccgc cccacctgac
     1021 ctcccggtgc tcgctgggtt gcccgccctc accgctgccg tcggtgcgcg ggggctcgct
     1081 gtcgtgcagg ctccgcccgg cacgggcaag acgacgatgg tgccgccggc actcgcggtg
     1141 ctcggcgcgg ggcgggtcgt cgtgacccag ccacggcgca tcgcggtgcg ggcggccgcg
     1201 cggcggctcg cgcagctgct cggtgagccg gtgggggaga gcgtggggta cgcggtgcgc
     1261 ggcgagcacc gcagcggtcg ggccacgcgg atcgagatcg tgacgacggg cgtcctgctg
     1321 cgacggctgc agcgcgaccc cgacctcccg ggtgtggacg ccgtcgtgct cgacgagtgc
     1381 cacgagcgag cgctcgacgg cgatctcgcg cttgcgctgc tggtggacgt gcgcacccac
     1441 ctgcgtccgg acctgacgct cgtcgcgatg tccgcgaccg tcgaggcgga acgcaccgcc
     1501 gacctgctcg gcgggaccga cccggcgccc gtggtcgcgg tcacgagcgc tctgcacccg
     1561 gtgacgcagg tctggtgccc gccgcctgcg cgggtcgcgc ggaccgacga gcgcggggtc
     1621 accccggggt ttctggacca tgtcgcggcg tgcgcgcgtc gtgcgctggc cgagtgcccg
     1681 ggtgacgtcc tggtgttcgt gcccggcgcg ggcgaggtca acggcgtggc gcgccggctc
     1741 gccgggatcg atgcggatct ccgcccgttg cacggacgcc tgtcccaccg ggaccaggac
     1801 ctcgcgctga cccccggtcc gcgtcgccgc gtcgtcgtct cgactgccgt ggccgagtcg
     1861 tcgttgaccg ttcccggggt ccgggtcgtc gtcgacgccg gcttctcccg cgagccgcgc
     1921 accgatcatc ggcgcgggct cgcgggactg gtcacggtcg gtgtgagccg ggcggccgcg
     1981 gaccagcgcg cgggccgtgc gggccgcgag ggaccgggtg cggtctaccg ctgctggtcg
     2041 ctcgccgagc acgcgcgcct gcgccaacat cccgagcccg agatcaggac ggccgatctc
//
LOCUS       DASRKH010000253         3081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_15515, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000253 DASRKH010000000
VERSION     DASRKH010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15515"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1148)
                     /locus_tag="VFC06_04105"
     CDS             complement(<1..1148)
                     /locus_tag="VFC06_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HZL81108.1"
                     /translation="MVTTSPRTARAAPRNAVDRYFKITERGSSIGAEVRGGLVTFFTM
                     SYIIVLNPIILGYVQDGTGKYLGGGDAPNLGMIAAATALIAGLLTILMGTVANFPIAL
                     AAGLGLNAVVAYSIAALPGMTWPDAMGIVVIEGLIILVLVLTGFRRAVFKAVPVEIKT
                     AISVGIGLFIAFIGLVDSGFVRIPASQATPVELGIGGSLAGWPLLVFVVGLLLAIVLM
                     VRKVKGAILIAILSSTVLALIVEAVGKVGSMKDGSGAVVKATGWELNVPHVSGSIVSV
                     PDFSLIGHFSLFGAVGKIGILPVALLVFAIMLADFFDTMGTMVAVGGEAGLLDDEGNP
                     AGAQQILIVDSIAAAAGGMGSVSSNTSYIESAAGVGDGARTGLASVVTG"
     gene            complement(1267..2253)
                     /locus_tag="VFC06_04110"
     CDS             complement(1267..2253)
                     /locus_tag="VFC06_04110"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HZL81109.1"
                     /translation="MQTTDRLPPLRRRDLRAYREGTLRRRSRRHVVAVVASVLGVALL
                     AALAPPVLNGDTPAEAVAALPARLAGLGDLAARGADLAVRGADVAIVAFTPPRDGGGG
                     GGARPSPSPVAMAAAGVREPSAAAQASPPPPASTVPQIAPQIASSATASSTASPTAPA
                     APEVAPPAAAPQTGLQAPQVGLQTAAPSVDAAVAGPAAAPTTAPAPTLTADEQFAARL
                     VTLTNAERAKAGLVALTTSPCATAQAAARAAVLVAQGRFEHDPLGPVLAACGGSSAGE
                     NLALGYPTPEAMTAGWMASPGHRENILRASTSIGIGCVTGSKGVLCSQVFLG"
     gene            complement(2336..2824)
                     /locus_tag="VFC06_04115"
     CDS             complement(2336..2824)
                     /locus_tag="VFC06_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81110.1"
                     /translation="MSDDTHLPAAKDVRDLLADLLGRDVQVTTGGPMVDPAVDFGAMV
                     GVYVDRNVRLAALVLLDIDLASRMGAAIALLPTRAAESAINNGLLPDNIRDNAAEILN
                     IMASLFNVEGAPHVLLDAVYAPGDPIPADVAQWILTFVRRCDLGVDVSGYGPGQFSLL
                     VP"
     gene            complement(2821..>3081)
                     /locus_tag="VFC06_04120"
     CDS             complement(2821..>3081)
                     /locus_tag="VFC06_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZL81111.1"
                     /translation="TGVEALAAVQADEPDLVLSDWNMPNMTGIELLGAMRSEGYGTPF
                     AFVTSEGSAEMREAAEQAGALFLISKPFTAESFRDAIEPVLA"
BASE COUNT          474 a         1080 c         1137 g          390 t
ORIGIN      
        1 ccggtcacga ccgacgcgag cccggtgcgc gcaccgtcac cgacgcccgc ggcggactcg
       61 atgtagctcg tgttggacga gacgctgccc atgccgccgg ccgccgcggc gatcgagtcg
      121 acgatgagga tctgctgagc gcccgcaggg ttgccctcgt cgtcgagcag cccggcctcg
      181 ccgccgacgg ccaccatcgt gcccatcgtg tcgaagaagt cggcgagcat gatcgcgaac
      241 accaggagcg cgaccgggag gatcccgatc ttgccgaccg cgccgaagag cgagaagtgc
      301 ccgatgagcg agaagtcggg tacggagacg atcgacccgg acacgtgggg gacgttgagc
      361 tcccagccgg tcgccttgac caccgcgccg ctgccgtcct tcatcgagcc gaccttgccg
      421 acggcctcga cgatgagcgc gaggaccgtc gagctgagga tcgcgatgag gatcgcgccc
      481 ttgaccttgc gcaccatgag gacgatcgcg agcagcaggc cgacaacgaa cacgaggagc
      541 ggccagcccg cgagcgaccc gccgatgccc agctcgaccg gtgtggcctg gctcgcgggg
      601 atccggacga acccggagtc gacgaggccg atgaacgcga tgaacagacc gatgccgacg
      661 ctgatggccg tcttgatctc gaccgggacg gccttgaaca cggcccgccg gaacccggtg
      721 agcacgagga ccaggatgat gaggccctcg atcacgacga tgcccatcgc gtcgggccac
      781 gtcatgcccg gcagggcggc gatcgagtac gcgaccacgg cgttgaggcc gagccccgcg
      841 gcgagcgcga tcgggaagtt cgcgacggtc cccatgagga tcgtcaggag gcccgcgatg
      901 agggccgtcg ccgcggcgat catgccgagg ttcggggcgt caccgccgcc caggtacttg
      961 cccgtgccgt cctggacgta gcccaggatg atcgggttga gcacgatgat gtagctcatc
     1021 gtgaagaagg tcacgaggcc gccgcggacc tcggcgccga tcgaggaccc acgctcggtg
     1081 atcttgaagt aacggtcgac ggcgttgcgg ggggctgctc tcgccgtccg gggggaggtt
     1141 gtcaccatgg cgcgcatgat ctcagaatcg gcaccgttcg cacgcaggtt gtccacgtgg
     1201 tgtgcgaact agcccatccg ggtgacaccg gtcgcgccgc ggtcccgcag tcgtccggcc
     1261 gcgcgatcag cccaagaaca cctgggagca gagcacgccc ttcgacccgg tgacgcagcc
     1321 gatgccgatc gaggtggagg cgcgcaggat gttctcccga tgacccggcg aggccatcca
     1381 gccggccgtc atcgcctccg gcgtcgggta gccgagtgcg aggttctccc ccgcgctgct
     1441 gcccccgcac gccgcgagga ccggcccgag cgggtcgtgc tcgaaccgcc cctgcgcgac
     1501 gagcacggcg gcccgcgcgg cggcctgggc ggtcgcgcac ggcgacgtcg tgagcgccac
     1561 gaggccggcc ttggcgcgct cggcgttggt gagcgtgacg agccgggccg cgaactgctc
     1621 gtcggcggtg agggtcggcg cgggggccgt ggtcggcgcc gccgccggcc cagcaaccgc
     1681 cgcgtcgacc gacggcgcag cggtctgcag accgacctgc ggagcctgca gccctgtctg
     1741 cggagcggcg gcgggcggag cgacctccgg cgcggccggt gctgtgggcg acgcggtgga
     1801 cgacgcggta gccgacgaag cgatctgcgg cgcgatctgc ggcacggtgg atgccggcgg
     1861 cggcggcgag gcctgcgcag cggctgacgg ctcacggacg ccagccgctg ccatcgcgac
     1921 cggcgacggg cttggccgcg cgccgcctcc ccctccaccg tcgcggggcg gggtgaacgc
     1981 cacgatcgcg acgtccgcgc cgcgaacggc caggtcagca ccgcgagcag ccaggtcgcc
     2041 gaggcccgcc aggcgggccg gcagtgccgc gacggcctcc gccggggtgt cgccgttcag
     2101 gacgggcggg gcgagcgccg cgagcagcgc gaccccgagg acggacgcca cgacagcgac
     2161 gacgtgccga cggctccgcc gacgcagtgt gccctcgcgg tacgcacgca ggtcacgtcg
     2221 ccgcagcggc gggagcctgt cggtcgtctg cacgtcggcc acgttatcgg ttcgtgacct
     2281 gcgagcggga gaccccgtcg tccgccggac ggccgacggc gcacccgacg gtgtgtcagg
     2341 gcaccagcag ggagaactgc ccggggccgt aacccgacac gtccacgccg aggtcgcagc
     2401 ggcgcacgaa cgtcaggatc cactgcgcga cgtccgcagg gatcgggtca cccggcgcgt
     2461 acacggcgtc gagcagcacg tgcggggcgc cctcgacgtt gaacagcgag gccatgatgt
     2521 tgaggatctc ggccgcgttg tcccggatgt tgtccggcag caacccgttg ttgatcgcgc
     2581 tctcggcggc gcgcgtcggc agcagggcga tcgctgcgcc catccgggag gccagatcga
     2641 tgtcgagcag cacgagtgcg gccagccgca cgttccggtc gacgtagacg ccgaccatgg
     2701 caccgaagtc gacggcgggg tcgaccatcg gtccgcccgt ggtgacctgg acatcccgcc
     2761 cgagcaggtc ggcgagcagg tcgcggacgt ccttcgcagc gggcaggtgc gtgtcgtcgc
     2821 tcatgcgagc accggctcga tcgcgtcacg gaacgactcg gccgtgaagg gcttcgagat
     2881 gaggaacagc gcgccggcct gctcggcggc ctcccgcatc tccgcggagc cctcggacgt
     2941 cacgaacgcg aacggcgtcc cgtagccctc cgagcgcatc gcgcccagca gctcgatccc
     3001 ggtcatgttg ggcatgttcc agtcggacag cacgaggtcg ggctcgtcgg cctggacggc
     3061 agccagggcc tccacgcctg t
//
LOCUS       DASRKH010000254         2413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_40962, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000254 DASRKH010000000
VERSION     DASRKH010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40962"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="VFC06_04125"
     CDS             <1..675
                     /locus_tag="VFC06_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81112.1"
                     /translation="LLDMYVESLDTGEVFENTYDGLPRSFLLAPELLGDDIYQVLLKA
                     RVGTRVLSIAPPLGEFDNEPAIVIVIDVLPDRAVGERLEVADTLPQMSVTDTGEPQIT
                     PNPDGVYPADLTISTLVQGAGAQIVPGSYIVAQIKAVYGTDGTKGDTSWKAGDVRQSS
                     WLPGQAPFEGQMGVAKQLRALEEGLIDQTAGSQVMLIAPEEWAYPGEGTLIYVIDILD
                     VWNGES"
     gene            675..1445
                     /gene="hisF"
                     /locus_tag="VFC06_04130"
     CDS             675..1445
                     /gene="hisF"
                     /locus_tag="VFC06_04130"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HZL81113.1"
                     /translation="MGVAVRVIPCLDVDAGRVVKGVNFANLRDAGDPVELAAQYGLEG
                     ADEVTFLDVTASSGGRETMYDVVRRTAEQVFVPLTVGGGVRTTDDVDSLLRAGADKVG
                     VNTAAIARPELISEIARRFGNQVLVLSVDARRCQGDTVTPSGYEVTTHGGRRGTGIDA
                     IEWAREGADRGAGEILLNSMDADGTTSGFDLAMIKEVRDVVSVPLIASGGAGRPEDFV
                     KAAQAGADAVLAASVFHFGTLSIRQVKEAMRSAGISVR"
     gene            1442..>2413
                     /locus_tag="VFC06_04135"
     CDS             1442..>2413
                     /locus_tag="VFC06_04135"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HZL81114.1"
                     /translation="MTAAPRAEYELSGSYSRKSLHVIGHALRTSPRRFAVSIGAAGIF
                     GVLTVVFGSVLGLIIDQVVIPAIDGEPIHGWWGERTQDPGTAVLLAGGVFVGIGLLNA
                     ALIVIRRAVQGGAVAAVGARHRTVVADAIAALPLGWHRANPTGRMLSAMSSDSETATN
                     PLHPLAFTVGSFTMMIAAGYRLVIMDTWIAVTAMMVVPAILIVNFLYEKVISPLWDAG
                     QSLRAEVSTIAHESFEGGTIVKALGAEARESARFATKVDGLRDADTRVGAVSSWFEPL
                     MDAMVPLSSLAIMVVGAHRAAAGAVTVGDVVTAIYLLALMAVPIRGLG"
BASE COUNT          383 a          745 c          858 g          427 t
ORIGIN      
        1 ctgctcgaca tgtatgtcga gtcactcgac acgggcgagg tctttgagaa cacctacgac
       61 ggactgcctc ggtcgttcct gctggcgccg gaactgctcg gcgatgacat ctaccaagtg
      121 ttgctcaagg caagggtcgg cacccgcgtc ctctccatcg cgccgccttt gggcgaattc
      181 gacaacgagc ccgccatcgt gatcgtgatt gatgtgttgc cggaccgcgc cgtgggggag
      241 cggctggagg tcgcggacac gcttccccag atgagcgtga ccgacacggg ggagccccag
      301 atcaccccca atccggacgg ggtgtacccc gcagacctca cgatctcgac gttggtccag
      361 ggcgccggcg cacagatcgt gccggggtcg tacatcgtgg cccagatcaa ggccgtctac
      421 ggtacggatg gcacgaaagg tgacacgtcg tggaaggcgg gcgacgtccg tcagtcgtcg
      481 tggcttccgg ggcaagcccc gttcgaaggt caaatgggcg tcgccaagca actgagggcg
      541 ctcgaagagg gcctgatcga ccagacggcc ggctctcagg tgatgttgat tgcgccggag
      601 gaatgggcct acccgggcga aggcaccttg atctatgtga tcgacatttt ggacgtgtgg
      661 aacggagaat cctgatgggc gtcgcggtgc gggtgattcc ctgcctggac gtggatgccg
      721 ggcgcgtggt gaagggcgtg aacttcgcca acctgcgcga cgcgggcgac cccgtggaac
      781 tcgccgcgca atatggcctt gaaggcgccg atgaggtgac cttcctcgac gtgacggcct
      841 cgtctggcgg ccgcgaaacg atgtacgacg tggtcaggcg caccgcggag caggtattcg
      901 ttccgctcac agtcggtggc ggggttcgga ccacggacga tgtggatagc ctcctgcgtg
      961 cgggcgcgga caaggtcggc gtgaacacgg ccgcgatcgc aaggccggaa ctcatctccg
     1021 agattgcgcg cagatttggc aaccaggtgt tggtgctgtc cgtcgacgcc cgacgatgcc
     1081 aaggcgacac cgtgacgccc tcggggtacg aggtcacgac ccacggcgga cgccggggca
     1141 cgggcattga cgccatcgag tgggcacgcg agggcgcgga caggggagcg ggagaaatcc
     1201 tgctcaactc gatggacgcg gacggcacca cgagtggttt cgacctcgcg atgatcaagg
     1261 aggtgcgtga cgtggtttcc gtgccgctca ttgcctcagg gggtgcgggg cgtcccgaag
     1321 actttgtcaa ggccgcccag gcgggtgcgg acgcggtgct cgcggcgagt gtgttccact
     1381 ttgggacgct ctcgatccgc caggtcaagg aagcgatgcg ctccgccggc atctcggtcc
     1441 ggtgacagcg gcaccccggg ccgagtacga gctgagcggg tcgtacagcc gcaagtccct
     1501 tcacgtgatc ggtcacgccc tgcgcacctc gccacgccgc ttcgccgtgt cgatcggcgc
     1561 ggcggggatc tttggcgtcc tcaccgtggt gttcggttcg gtgctggggc tcatcatcga
     1621 tcaagtcgtg atccccgcga ttgatggcga gcccatccac gggtggtggg gcgaacgcac
     1681 gcaggacccg ggcacggccg tgctcctcgc tggaggagtc ttcgtcggca tcgggctgct
     1741 gaacgcggcg ctgatcgtga tccgccgggc cgtccagggc ggcgccgttg ccgcggtggg
     1801 cgcccgacac cgcacggtgg tggccgacgc gatcgcggcg ctgccgctcg ggtggcatcg
     1861 cgccaacccc accggtcgca tgctgtccgc gatgtcttcg gactcggaga ccgcgaccaa
     1921 cccgctgcac ccgcttgcgt tcaccgtggg gtcattcacg atgatgatcg cggccggata
     1981 ccggctggtc atcatggaca cgtggatcgc cgtcaccgcg atgatggtgg ttcccgcgat
     2041 cctgatcgtg aatttcttgt acgagaaggt aatcagccct ttgtgggacg cgggccaaag
     2101 cctgcgcgcg gaggtctcga ccattgcgca cgagtcgttc gagggcggca ccatcgtcaa
     2161 ggctctgggg gccgaggctc gggagtcggc gcgtttcgcc accaaggtcg acggacttcg
     2221 cgacgccgac accagggtgg gcgccgtgtc gagttggttt gagccgctca tggacgccat
     2281 ggttccgctc agctcgctcg ccatcatggt ggttggcgcg caccgggccg ctgcgggcgc
     2341 tgtcaccgtg ggcgacgtgg tgacagcgat ctacctcttg gcactcatgg ccgttccgat
     2401 tcggggcctg ggg
//
LOCUS       DASRKH010000255         2722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_14734, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000255 DASRKH010000000
VERSION     DASRKH010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14734"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..850)
                     /locus_tag="VFC06_04140"
     CDS             complement(125..850)
                     /locus_tag="VFC06_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HZL81115.1"
                     /translation="MARLTGRSGLARLSPVRQQALSVSLATGAYGLSLGALAVAAGLD
                     FWQTMALSLIVFSGGSQFAFVGVIGAAGSPWAALGTSALLGVRNGFYGVTLAPILRPM
                     RGWRFVWGSQVTIDESTAVALSQPEHDANARRTGFWWTGVGVFVAWNLLVGVGAIAGQ
                     YIGEPQAWGLDAAAAAAFLGLVWPRLAPRRAQAVAAAAAVLALALTPVLPAGLPILAA
                     ALVAVGAGWGGPRVDLDPTEAPV"
     gene            complement(838..1476)
                     /locus_tag="VFC06_04145"
     CDS             complement(838..1476)
                     /locus_tag="VFC06_04145"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZL81116.1"
                     /translation="MPKTRWRIPHRIVTERLTLRRYTADDAAEMSRVTLASKEHLVPF
                     MPWARAEPLPPEERAALLDTLMAEFDEGRDFTMGIFLREENVYVGGTGLHTRLGPGVL
                     DIGYWIAHDQQGHGYVTEATLALTHVALDLAGAERVEIHHSPDNLASRAVPQRCGYSY
                     DGRKLTLMQGVPDVEPTDMWSATSQQLASEPLASTPRPRVFDAHGQELSWPA"
     gene            complement(1514..2038)
                     /locus_tag="VFC06_04150"
     CDS             complement(1514..2038)
                     /locus_tag="VFC06_04150"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HZL81117.1"
                     /translation="MTIKVIWAQARDQAGRPVIGRDGDMPWHLPEDLAHFQECTRGHT
                     VVMGRRTWESLPPKFRPLPGRDNVVVTRSGAAFHGAASASSLASALECAKRVSSNTDV
                     WIIGGARLFTAAARLADEAVVTDIDLVAEGDTFAPTLNPAEWAVESTSEPLVSRTGLG
                     YRFVTYRRAAGSDA"
     gene            complement(2043..>2722)
                     /locus_tag="VFC06_04155"
     CDS             complement(2043..>2722)
                     /locus_tag="VFC06_04155"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015776901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HZL81118.1"
                     /translation="DRGFPLLTTKRVHVRSIVGELLWFLRGDTNVRWLQERGITIWNE
                     WADADGELGPVYGYQWRSWPAPDGRHIDQIANVIESIKTNPDSRRHIVSAWNPALIDE
                     MALPPCHALFQFYVADGKLSCQLYQRSADLFLGVPFNIASYALLTHMIAAQTGLDVGD
                     FVWTGGDCHIYSNHMDQVELQLSRAPYPYPTLRLAQKESLLDYDIDDIEIVDYQHHPG
                     IKAPIAV"
BASE COUNT          435 a          875 c         1004 g          408 t
ORIGIN      
        1 ctcgcgatgc gatgcacgcg cgggtgttcg agccaccgtt cgggcaccag gtgacccgtg
       61 gccttggtga gccacgagac cacgccagcc gccacgattg cgacccacag ccacgcgtac
      121 gagctcatac gggggcctcc gtcgggtcga ggtcgacgcg cgggccgccc cagcctgcgc
      181 cgaccgccac cagcgccgcg gcgagaatcg gcaggccagc ggggagcacg ggagtgaggg
      241 cgagggcgag cacggctgct gcggcggcga cggcttgggc tctgcgcggc gcgaggcggg
      301 gccagacgag gccgaggaat gcggccgccg ccgctgcgtc gagtccccac gcctgcggtt
      361 cgccgatgta ctggccggcg atcgcgccca cgccgactag gaggttccag gcgacgaaca
      421 cgcccactcc ggtccaccag aagcccgtgc gcctggcgtt cgcatcgtgc tccggctgtg
      481 agagcgccac ggcggtggac tcgtcgatcg tgacttgcga gccccacacg aaccgccacc
      541 cgcgcatcgg ccgcaggatg ggagccaacg tgaccccgta gaagccgttg cgcacgccta
      601 ggagcgcgga ggtgcccagg gcggcccacg gggagcccgc cgcgccgatg acgcccacaa
      661 aagcgaactg ggagccgccg gagaacacga tgagcgacag cgccatggtc tgccagaagt
      721 cgagcccggc ggcgaccgcg agcgcgccga ggctgagccc gtacgcgccc gtcgcgagcg
      781 agaccgagag agcctgctgg cgcactgggc tgaggcgggc gagccccgag cgtccggtca
      841 ggcgggccac gacaactcct ggccgtgggc gtcgaagacg cgcgggcggg gggtcgaggc
      901 gaggggttcg ctcgcgagtt gttgtgacgt ggcggaccac atgtccgtgg gctccacgtc
      961 agggacgccc tgcatgaggg tgagcttgcg gccgtcgtag gagtagccgc agcgctgagg
     1021 cacggcgcgt gacgcgaggt tgtccggact gtggtggatc tccactcgct ccgcacccgc
     1081 caggtcaagg gcgacatgag tgagggcgag cgtcgcctcg gtcacgtacc cgtgaccctg
     1141 ctggtcgtgc gcgatccagt agccgatgtc gagcacgcca ggaccgagcc gggtgtggag
     1201 gccagtgcct ccgacataga cgttctcttc gcgaaggaaa atgcccatcg tgaagtcgcg
     1261 cccctcgtca aactccgcca tcaaggtgtc gagcagagcg gcgcgttcct cgggtggcaa
     1321 gggttcggca cgcgcccacg gcatgaacgg gacgaggtgt tccttgctcg cgagcgtcac
     1381 ccgcgacatt tcggccgcat cgtcggcggt gtaccgacgc agtgtcaggc gttccgtgac
     1441 gatgcggtgc gggatgcgcc accgggtctt aggcatgcgc cgattctggc acgcctggcg
     1501 ggcgttcagg cgttcaggcg tccgagcccg cagcgcgccg gtaggtgacg aagcggtagc
     1561 cgagtccggt gcgggacacg agcggttcgg aggtcgactc cacggcccac tcggcggggt
     1621 tgagcgtggg ggcgaaggtg tcgccttcgg cgaccaggtc aatgtcggtg acgaccgcct
     1681 cgtcggcgag ccgtgccgcc gccgtgaaca gcctcgcgcc gccgatgatc cacacgtcgg
     1741 tgttggacga cacgcgtttg gcacattcga gtgcggaggc cagcgaactg gcgctcgcgg
     1801 ctccgtggaa ggcggcgccg gagcgggtca ccaccacatt gtccctaccc ggcagcggcc
     1861 tgaacttggg ggggagcgac tcccacgtgc gtcgtcccat gaccacggtg tgcccgcggg
     1921 tgcactcctg gaagtgggcg aggtcctcgg gaaggtgcca cggcatgtcg ccgtcgcgtc
     1981 cgatgacggg gcggccagcc tggtcgcgcg cctgagccca gatgaccttg atcgtcacgg
     2041 cgttagacag caatcggggc tttgatgccc gggtgatgct ggtagtcgac gatctcaatg
     2101 tcgtcgatgt cgtaatccag cagcgactcc ttctgagcga ggcgcagcgt ggggtagggg
     2161 tacggcgctc gcgagagttg cagctcgacc tggtccatgt ggttggagta gatgtggcag
     2221 tcaccgccgg tccacacgaa gtcgcccacg tcgaggccgg tctgggccgc aatcatgtgc
     2281 gtcagcaggg cgtacgaagc gatgttgaag ggcacgccca ggaacaggtc cgcgctgcgc
     2341 tggtacagct ggcagctgag tttgccgtcg gccacataga actggaagag cgcgtggcac
     2401 ggtggcagcg ccatctcgtc gatcagcgcg gggttccacg cggagacgat gtgccggcgc
     2461 gagtcggggt tggtcttgat ggactcgatc acgttggcga tctggtcgat gtgtcggccg
     2521 tccggggctg gccaggagcg ccactggtag ccgtagaccg ggccaagctc gccgtcggcg
     2581 tccgcccact cgttccagat ggtgatgccg cgttcctgca accagcgcac gttcgtatcg
     2641 ccgcgcagga accacaacag ttcgccgacg atggagcgca cgtggaccct cttggtggtg
     2701 agcaacggga agcccctgtc tc
//
LOCUS       DASRKH010000256         2178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_53587, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000256 DASRKH010000000
VERSION     DASRKH010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53587"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..1153)
                     /gene="rarD"
                     /locus_tag="VFC06_04160"
     CDS             complement(188..1153)
                     /gene="rarD"
                     /locus_tag="VFC06_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HZL81119.1"
                     /translation="MTEALDRRGLAYGASAYVVWGLFPILMVALKPAGAFEIVAWRAL
                     SSLLVCIAILAAMGAWRRLRAVASHRPTVLRLSAASVLIALNWGVMVYAVVTDRVAHT
                     SLGYYINPLITVGLSVLFLGERLRKLQMVAISLGVVAVVVIAVEMGGLPWISLVLACS
                     FAGYSFIKKGVGGRVDALSGLAIETAVLAPVALGVLWAVGANGDATFLARGADGLGWW
                     HDVMMVGTGTWTAGALLVFAAGARRLPLNVTGMLQYIAPTMTFALAVWYFHEPMPAGR
                     WAGFGLVWIALTLITIDAWRARPISKRRAAGLARAAGEQPVTEPI"
     gene            complement(1150..1491)
                     /locus_tag="VFC06_04165"
     CDS             complement(1150..1491)
                     /locus_tag="VFC06_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81120.1"
                     /translation="MLVSQYRPTSELGRVIESRPHEMWFNPPRNASLFNCLLISASMA
                     VTRHRTLYRKRRYLHAKAIVFYLKSGKRVAISGSHNFVRGGVTLGTAGPRRAEPVSRV
                     AASAPNATLSL"
     gene            complement(1504..>2178)
                     /locus_tag="VFC06_04170"
     CDS             complement(1504..>2178)
                     /locus_tag="VFC06_04170"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HZL81121.1"
                     /translation="QWPMPGPGPARVKDDRLLERADLLLPQEYIADATRSIHDATRRV
                     RVIALTIDDEAETGALLDALVAAARRGVDVHVAADTFTYADAAGRFVPKSYLTKRRRS
                     SMDMAKELTDAGATFDWLGQEGGLPWRGRTHTKFCVVDDTVYSFGGVNLDAQGATNAD
                     FMLRITDHRLAEDLVRVYARTRRANSRFSGFRSSALKYGKDQVLVDGGIPGDSIIYRR
                     ALKYAR"
BASE COUNT          388 a          723 c          764 g          303 t
ORIGIN      
        1 gcggcgacct tgccgatctg gacaacatgg acgagtacgc cacgggagca agggccgtgg
       61 tggagctggc tgcgcgtgac aagcgcgtcg aggcgaccgt gctgcagaca gtcggccgca
      121 aggactacga cggaatgctc atcgctacgg tgctgtagag ggccgcatgc cacctcacgg
      181 tgggctgcta gatcggctcg gtcaccggct gctcgccagc ggcgcgagcg agcccagcgg
      241 ccctgcgttt tgatatgggc cgcgcgcgcc acgcgtcaat ggtgatgagg gtgagcgcaa
      301 tccacaccag gccgaagccc gcccagcgac cggcgggcat cggctcgtgg aagtaccaca
      361 cggcaagcgc aaaggtcatg gtcggcgcga tgtactgcag catccctgtg acgttcagcg
      421 gcagcctgcg agcgccggcg gcgaatacga gcagcgcgcc tgcggtccac gtgccggtgc
      481 caaccatcat gacgtcgtgc caccacccca agccgtcggc cccgcgggca aggaacgtgg
      541 cgtccccgtt cgcacccacg gcccacagca ccccgagggc caccggcgcg agcaccgccg
      601 tctcgatggc cagaccgctc agcgcgtcca cccggccgcc cacgcccttc ttgataaacg
      661 agtagccggc aaaggagcac gccagcacca aggagatcca cggcaggccg cccatctcta
      721 cggcgatcac caccacggcg acgacgccga gcgagatggc gaccatctgc agcttgcgca
      781 gacgctcgcc gaggaacagg acgctgagcc ccaccgtgat caaggggttg atgtagtagc
      841 cgagcgaggt gtgggccacg cggtcagtga cgacggcgta gaccatcact ccccagttca
      901 gggcgatgag taccgaggcc gcagagagcc gcagcactgt gggccggtgg ctggccacgg
      961 cgcgcaagcg acgccaggcg cccatggcgg cgaggatggc gatgcagacc agcagggagg
     1021 acagggcgcg ccaggccaca atctcgaagg cccctgccgg cttgagggcg accatcagga
     1081 tggggaagag gccccacacc acgtaggcgc tggcgccgta ggcgaggccg cggcggtcca
     1141 gggcttctgt catagcgaca gtgtggcatt gggcgctgag gccgcgacgc gcgaaactgg
     1201 ctccgcacgc cgggggccag cggtgccgag cgtcaccccg ccgcgcacaa agttgtggga
     1261 gcccgagatt gcgacgcgct tgccggactt caggtagaac acgatcgcct tggcgtgcag
     1321 gtaccgccgc tttcggtaca gagtgcggtg gcgcgtgacc gccatcgacg cggaaatcag
     1381 aaggcagttg aagaggctcg cgttgcgcgg cgggttgaac cacatctcgt gaggcctgga
     1441 ctcgatcacc cgacccagct cgctggtggg gcggtactgg gacaccagca cgatgcgctg
     1501 ggactagcgt gcgtacttga gcgcgcggcg atagatgatc gagtcgccag ggatcccgcc
     1561 gtcgacgagc acctggtcct tgccatactt cagcgcggac gaccggaagc cggagaagcg
     1621 cgagttggcg cgccgggtgc gcgcgtatac ccgcaccaag tcctcggcca ggcggtggtc
     1681 ggtgatgcgc aacatgaagt ccgcgttggt cgctccttga gcgtccagat tgactccgcc
     1741 aaagctgtag acggtgtcgt cgaccacgca gaacttggtg tgcgtgcgcc cgcgccacgg
     1801 caggccgccc tcttgaccca gccagtcaaa ggtcgcgccc gcgtccgtca gctcctttgc
     1861 catgtccatc gacgagcgcc gccgcttcgt caggtaactc ttcggcacga agcggccagc
     1921 ggcatcggcg taggtgaagg tgtcggcggc gacgtggacg tccacgccgc ggcgcgcggc
     1981 cgctaccagg gcgtcgagca aggctccggt ctcggcctca tcgtcgatcg tgagcgcgat
     2041 gacccgaaca cgacgcgtgg cgtcgtgaat gctccgggtg gcgtcggcga tgtactcctg
     2101 aggcagcagc aggtccgcgc gctccaggag gcggtcgtcc ttgacgcgcg ctggccccgg
     2161 gcccggcatg ggccactg
//
LOCUS       DASRKH010000257         2271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_38364, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000257 DASRKH010000000
VERSION     DASRKH010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38364"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..689
                     /locus_tag="VFC06_04175"
     CDS             156..689
                     /locus_tag="VFC06_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HZL81122.1"
                     /translation="MTDSRYLQLLREQIGHEFDAHQQYVAIAVWFDGQDLPQLARHYY
                     RQAIEERNHALMIVRYLLDRDLHVEIPAGGPVRNDFTTVLDPISLALAQEQQVTEQIE
                     AIFRAAREEGDALGEQFLLWFLKEQVEEVASATTLRAVAKRAGDNLFDLENYVAREQI
                     GDGGESADAPAAAGGAL"
     gene            737..1237
                     /locus_tag="VFC06_04180"
     CDS             737..1237
                     /locus_tag="VFC06_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HZL81123.1"
                     /translation="MLLRRIARPLFASWFVSEGYDATRHPEVHAERARAGLESLVRLV
                     PRGALGGALDRYRQPTTAQLVTLVRAHGAATTAAGVLLAAGKAPRTAALALAALTAPL
                     ILANLPLRDRPALSKDARSDRRDRLVRAVAFTGGALLAGADYEGRPGVAWRVAKAREE
                     HASAAQ"
     gene            1319..>2271
                     /locus_tag="VFC06_04185"
     CDS             1319..>2271
                     /locus_tag="VFC06_04185"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HZL81124.1"
                     /translation="MTTPETLDPVGLGRDAWDAPLAPGPLDATVEVPGSKSLTNRYLV
                     LAALADAPGRLRGALRSRDTLLMADALRALGTRIEDAPGDSDDWVVTPTHPRAPATIS
                     CGLAGTVMRFLPAVAALADGPVQFDGDPGARVRPMGPVLAALRTLDVRVEEHGEPGHL
                     PFTVTGRGSVRGGHIDVDASGSSQFVSGLLLAAARFEQGLTLRHIGPTLPSLPHIDMT
                     VQLLRAAGVDVDDSRPAIWHVTPGPIRGRDVRVEPDLSNAAPFLCAALVAGGTVRVPG
                     WPTQTTQPGGLLPGILERMGATTRLEGDVLAVSGTGAIHGV"
BASE COUNT          315 a          913 c          752 g          291 t
ORIGIN      
        1 gcgagtgcac agctcatgat gcgcacaacg cagcgtggta ccccacgatt cccgggccgg
       61 ttcgtaccct ccgcgtccgc cacccgtccc ggggaccatc cgacgctctc ccgccccgct
      121 cggcagacgt atgccgtgag cgctagcgtg acgtcatgac tgactcgaga tacctgcagc
      181 tcctccgcga gcagatcggc cacgagttcg acgcccacca gcagtacgtc gcgatcgccg
      241 tctggttcga cggtcaggac ctgccccagc tcgctcggca ctactaccgc caggcgatcg
      301 aggagcgcaa ccacgcgctg atgatcgtcc ggtacctgct cgaccgggac ctgcacgtcg
      361 agatcccggc cggcggaccg gtgcgcaacg acttcacgac ggtcctcgac cccatctccc
      421 tcgcgctcgc gcaggaacag caggtcacgg agcagatcga ggcgatcttc cgggccgctc
      481 gcgaggaagg cgacgcgctg ggcgagcagt tcctcctgtg gttcctcaag gagcaggtcg
      541 aagaggtcgc ctccgccacg acgctgcgcg ccgtcgcgaa gcgcgcaggc gacaacctgt
      601 tcgacctcga gaactacgtc gcccgcgagc agatcggtga cggcggcgag tccgcggacg
      661 cgcccgcggc agccggtgga gccctctgag cgcggcctgc gcccgcaccc gccctgtgcc
      721 gtcaaggaga accgacatgc tgctccgccg aatcgcccgt cccctgttcg cctcatggtt
      781 cgtatccgag gggtacgacg cgacgcggca tcccgaggtg cacgcagaac gcgcccgcgc
      841 cggcctcgag agcctcgtcc gtctcgtccc gcgcggtgcg ctcggcggcg cgctggaccg
      901 ctaccggcag cccacgaccg cccagctcgt gacactcgtg cgcgcgcacg gcgcagcgac
      961 gaccgcggca ggcgtcctgc tcgcggccgg caaggcgccc cgcaccgcag ccctcgccct
     1021 cgccgcactc accgcaccgc tcatcctcgc gaacctgccg ctccgcgacc gcccagcgct
     1081 cagcaaggac gcacgcagcg atcgcaggga ccgcctggtg cgcgccgtcg cgttcaccgg
     1141 aggcgcgctc ctcgccggcg ccgactacga gggtcgtccc ggcgtggcgt ggcgcgtcgc
     1201 gaaggcacgc gaggagcacg cgtccgcagc gcagtgaccg agcacggcgg atcgccgcac
     1261 gggccgctgc cggatcgttg cccaccggcg ccggccctcg ggccgatagc ctgaccccat
     1321 gacgacgccc gagactctcg accccgtcgg actcggccgg gacgcgtggg acgcaccgct
     1381 cgcgccgggc ccgctcgacg ccaccgtcga ggtccccggc tcgaagtccc tcaccaaccg
     1441 ctacctcgtc ctcgccgcgc tcgcggacgc gcccgggcgg ctgcgcggcg cgctgcgctc
     1501 acgcgacacc ctgctcatgg ccgacgctct gcgcgcgctc ggcacccgca tcgaggacgc
     1561 gccgggcgac agcgacgact gggtcgtgac gccgacccat ccccgcgcac ccgcgacgat
     1621 cagctgcggg ctcgcgggca cggtgatgcg cttcctcccc gccgtcgcgg cgctcgctga
     1681 cgggcctgtg cagttcgacg gcgaccccgg ggcgcgcgtg cgacccatgg gacccgtgct
     1741 cgccgccctg cgaaccctcg acgtgcgcgt cgaggagcac ggcgagccgg gacatctccc
     1801 cttcaccgtg accggccgcg gctcggtccg cggtggccac atcgacgtgg acgcctcggg
     1861 ctcgagccag ttcgtctcgg gcctgctgct cgccgccgcg cgcttcgagc aggggctgac
     1921 gctccgacac atcggaccga cgctgccgag cctgccgcac atcgacatga ctgtgcagct
     1981 gctccgggcc gccggggtcg acgtcgacga ctcccgcccc gcgatctggc acgtcacgcc
     2041 gggcccgatc cgtgggcgcg acgtacgcgt cgaacccgac ctctccaacg cggcaccgtt
     2101 cctgtgcgcc gcactcgtcg ccggcgggac cgtccgcgtg cccggttggc ccacgcagac
     2161 cacgcagccc ggcggcctcc tgcccggcat cctcgagcgc atgggagcca ccacacggct
     2221 cgagggtgac gtgctcgcgg tgagcggcac cggcgcgatc cacggcgtcg a
//
LOCUS       DASRKH010000258         2128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_28528, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000258 DASRKH010000000
VERSION     DASRKH010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28528"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2128
                     /locus_tag="VFC06_04190"
     CDS             <1..>2128
                     /locus_tag="VFC06_04190"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HZL81125.1"
                     /translation="LVRRGALEIYGPPEAERARGAYAQLAHELRIIEDLGFPGYFLIV
                     YDLVDFCRRNGILAQGRGSAANSAVCYALRVTAVDAVKHGLLFERFLAPERDGPPDID
                     VDIESERREEVIQYVYATHGRTHAAQVANVISYRPRSAVRDAARALGHDVGQQDAWSK
                     SIDRWGSLRGPDPVTADGRDAATGREERAKHRDAMWGAAPEPPAHDLDEIPAPVLDLA
                     QRFLRLPRHLGIHSGGMVMCDRPVIEVCPVEWARMPGRTVLQWDKEDCADAGLVKFDL
                     LGLGMLTALRIAFDLVREHEGVSLDLHGLPHEDPLVYDLLCAADTVGVFQVESRAQMG
                     TLPRLEPRRFYDIVVEVALIRPGPIQGGSVHPYINRVHGREPVTYLHPKLEKSLAKTL
                     GVPLFQEQLMQMAIDVADFTPTEADQLRRAMGSKRSTERMEALHARLLEGMAANGITA
                     EIGEQIFDKLKAFADFGFPESHAYSFAYLVYASAWLKVHHPAAFYAGLLAAQPMGFYS
                     PQSLVADARRHGLTVLRPDVQASGVRATVERTASAPVGGEPRLVPTPSGTAPTDQHAG
                     GSAGRSLAVRLGLASVRNVGAEVAARLVAERAQAGLFADLRDLARRVRLSTSHLEALA
                     TGGALDSLGATRREALWAAGALAQEGPGTLPGVSVGVIAPMLPGMSDVELAVADVWAT
                     GISVDSYPTQHVRARGPQCLPTRRTEA"
BASE COUNT          283 a          756 c          782 g          307 t
ORIGIN      
        1 agctggtccg gcgcggcgcc ctggagatct acgggccgcc cgaggccgag cgggcccggg
       61 gcgcgtatgc gcagctcgcg cacgagctgc gcatcatcga ggacctcggg ttcccggggt
      121 acttcctcat cgtctacgac ctcgtcgact tctgtcgtcg gaacgggatc ctcgcgcagg
      181 gccgcggctc ggctgcgaac tcggcggtct gctacgcgct gcgcgtcacg gcggtcgacg
      241 cggtcaagca cgggctgctc ttcgagcgct tcctcgcccc cgagcgggac ggcccgcccg
      301 acatcgacgt cgacatcgag tcggagcggc gcgaggaggt catccagtac gtctacgcca
      361 cgcacggtcg tacacatgct gcacaggtcg cgaacgtcat ctcctaccgg ccccgctcgg
      421 cggtccggga cgccgcacgc gcgctgggcc acgacgtcgg gcagcaggac gcctggagca
      481 agtcgatcga ccggtggggg agcctgcgcg gaccggaccc ggtcaccgcg gacgggcggg
      541 acgcagcgac gggtcgagag gagcgcgcca agcaccggga cgcgatgtgg ggggcggcac
      601 ccgagccacc cgcgcacgac ctcgatgaga tcccggcgcc cgtgctcgac ctcgcgcaac
      661 ggttcctgcg gctgccgcgt catctcggca tccactcggg cggcatggtc atgtgcgacc
      721 gaccggtcat cgaggtgtgc ccggtcgagt gggcccggat gccgggccgc acggtgctgc
      781 agtgggacaa ggaggactgt gccgacgcgg gtctcgtgaa gttcgacctg ctcgggctcg
      841 gcatgctcac ggcgctgcgg atcgcgttcg accttgtgcg cgagcacgag ggggtgagcc
      901 tcgacctgca cgggctgccg cacgaggacc cgctcgtcta cgacctgctg tgcgcggcgg
      961 acacggtcgg ggtgttccag gtcgagtcgc gggcccagat ggggactctg ccgcgcctgg
     1021 agccccgacg cttctacgac atcgtcgtcg aggtcgcgct catccgaccg gggccgatcc
     1081 agggcgggtc ggtgcacccg tacatcaacc gggtccacgg tcgggagccg gtcacgtacc
     1141 tgcacccgaa gctcgagaag tcgctcgcga agaccctcgg cgtgccgctg ttccaggagc
     1201 agctcatgca gatggcgatc gatgtcgccg acttcacccc gacggaggcc gaccagctgc
     1261 gccgcgcgat ggggtccaag cggtcgaccg agcgcatgga ggcgttgcac gcgcggctgc
     1321 tcgaggggat ggcagccaac ggcatcacgg ccgagatcgg tgagcagatc ttcgacaagc
     1381 tcaaggcctt cgcggacttc gggttccccg agtcgcacgc gtactcgttc gcctacctgg
     1441 tctatgcgag cgcctggctc aaggtgcacc acccggcggc gttctatgcg gggttgctcg
     1501 ccgcacagcc gatgggcttc tactccccgc agtcgctcgt ggccgacgcg cgtcggcatg
     1561 gtctgacggt gctgcgcccg gacgtgcagg cgtccggggt ccgggcgacc gtcgagcgca
     1621 cggcgtcggc gccggtcggc ggtgagcccc ggctcgttcc caccccgtcc gggacggcgc
     1681 cgaccgacca gcacgccggc gggtctgcgg gtcgctcgct cgcggtgcgg ctcggtctcg
     1741 catcggtccg caacgtcggc gcggaggtcg cggcgcggct cgtcgcggag cgcgcgcagg
     1801 ccggactgtt cgccgacctg cgcgacctgg cccgtcgggt gcggctgtcg accagccacc
     1861 tcgaggcgct cgcgacgggt ggtgcgctcg acagcctcgg tgcgacgcgt cgggaggcac
     1921 tgtgggccgc gggcgcgctc gcacaagagg gccccggcac gctgcccggc gtgtcggtcg
     1981 gggtcatcgc ccccatgctc cccggcatga gcgacgtcga gctcgcggtc gcggacgtgt
     2041 gggcgacggg catctcggtc gactcctacc ccacgcagca cgtgcgcgcc cgcggcccac
     2101 agtgcctccc gacgcgtcgc accgaggc
//
LOCUS       DASRKH010000259         2585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_49246, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000259 DASRKH010000000
VERSION     DASRKH010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49246"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VFC06_04195"
     CDS             complement(<1..485)
                     /locus_tag="VFC06_04195"
                     /inference="COORDINATES: protein motif:HMM:NF024135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HZL81126.1"
                     /translation="MKVLVIAASKHGATKEIGTAIVEELAIAGLDAVQAEPGDVTTLD
                     GVDAVVVGSAVYMTQWMETIRNFISHYGPQLRELPLWAFSCGLSGVPIGNVQDPRRIG
                     PALLSINPIDHQTFKGRMEFSGLSLRERSIARLGNAPEGDYREWDKIRAWAQQIAADL
                     K"
     gene            complement(512..1309)
                     /locus_tag="VFC06_04200"
     CDS             complement(512..1309)
                     /locus_tag="VFC06_04200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZL81127.1"
                     /translation="MMAPPAPRTALITGASRGIGRHLALGFAAAGLDVGLIATSPDGL
                     AGVAAEIASACDARVVTVAADVTREVDVVRAVAEVTRGLGSIDVLVNNAGRVDAEVPL
                     WEADPEEWWDVVAVNVRGPFLLAHAVVPGMLAGGGGRVIDINSGSGTRDFATSTGYTA
                     SKSALFRIGGALHEAGFALGLRAFEMAPGVVRTDMTGSMAMHAGRTQWTDPADVVALA
                     LALARGEGDHLSGSYLRVGLDRPEELRSRGSAQRLGLVPSEARKFSG"
     gene            complement(1306..>2585)
                     /locus_tag="VFC06_04205"
     CDS             complement(1306..>2585)
                     /locus_tag="VFC06_04205"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF036799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HZL81128.1"
                     /translation="VVTDVDTTAPPRPVVMDGRLPARVVGVDYAVEDVVATLTMLGAG
                     VERDGDTLTVTPPSWRTDLAIPEDLVEEVARLRGYDEIPSVVPAAPAGRGLTDRQRQR
                     RAVMRSLAEHGLNEVLSYPFVGDVHDRLHVPADDPRRRMVRLVNPIADDAPFMRSTLL
                     ASLIEVARRNVGRGNDLAIMEIGLVTEGADVASSPLPPVAQLPDPGTLAAIHAAVPAQ
                     PLHVAAMMAGPPAPGGALGGAREFDWADAVELAQLAGRTVGVPIASRQGDRTPWHPGR
                     CAELVVAGAVVGYAGELHPAVLPGLDLPPRTVAFELDLDALFAAVPVDALQVRPVSTY
                     PQAKEDIALVMDAAIPNGHALDVVRGAAGVLAEEVRLFDEYTSAALGAGLKSLAFALR
                     LRAADHTLTAAEIAGVRETVIARATSALGARLR"
BASE COUNT          370 a          956 c          923 g          336 t
ORIGIN      
        1 tccttgaggt cggcggcgat ctgctgcgcc caggcacgga ttttgtccca ctcgcggtaa
       61 tcgccctcgg gagcgttgcc gaggcgggcg atggaccgct cgcgcaggga caggccggag
      121 aactccatcc tgcccttgaa cgtctggtgg tcgatcgggt tgatggagag cagggccggc
      181 ccgatacgcc gcgggtcctg gacgttgccg atcggcacgc ccgagaggcc gcacgagaac
      241 gcccagagcg ggagctcccg caactgcgga ccgtagtggg agatgaagtt ccggatggtc
      301 tccatccact gcgtcatgta gacggcggac ccgacgacca cggcgtccac gccgtcgagc
      361 gtggtcacgt cccccggctc cgcctggacc gcgtcgaggc cggcgatcgc gagttcctcg
      421 acgatggccg tgccgatctc cttcgtggca ccgtgcttgg atgccgcaat taccaggacc
      481 ttcatgggcc cattctctcc cgatttctga ttcatccgga aaactttcgc gcctcggacg
      541 gtacgaggcc gagccgttgc gccgacccgc gggacctgag ctcctccgga cggtcgaggc
      601 cgacgcgcag gtaggagccg gacaggtggt ccccctcgcc gcgggcgagg gcgagggcga
      661 gggcgacgac gtcggccggg tcggtccact gggtgcgccc cgcatgcatc gccatcgagc
      721 cggtcatgtc cgttctgacc acgcccggcg ccatctcgaa ggcgcgcagg ccgagtgcga
      781 acccggcctc gtgcagtgcg cccccaatgc ggaacagcgc cgacttcgag gcggtgtacc
      841 ccgtgctggt ggcgaagtcg cgcgtcccgg acccggagtt gatgtcgatg acgcgccccc
      901 cgccgcccgc cagcatcccc gggaccaccg cgtgggccag caggaacggc ccgcgcacgt
      961 tcaccgcgac gacgtcccac cactcctccg ggtcggcctc ccagagcggc acctccgcgt
     1021 cgacacgccc ggcgttgttg acgagaacgt cgatcgaccc gagcccgcgc gtcacctcgg
     1081 cgaccgcacg gacgacgtcg acctcccgcg tgacgtccgc cgccacggtg acgacacgcg
     1141 cgtcgcaggc agaggcgatc tcggccgcca cccctgcgag cccgtccggc gaggttgcga
     1201 tgagtccgac gtccaacccg gcggctgcga agcccagcgc cagatggcgt ccgatgcccc
     1261 gcgaggcgcc cgtgatgagg gccgtgcgcg gggcgggcgg cgccatcatc ggagccgtgc
     1321 ccccagcgct gaggtcgcac gggcgatgac ggtctcacgc accccggcga tctccgccgc
     1381 cgtcaacgtg tggtccgcgg ctcgcagccg cagcgcgaac gccagcgact tgagccccgc
     1441 gccgagcgcc gctgacgtgt actcgtcgaa cagcctcacc tcctccgcga gcacccccgc
     1501 ggcgccgcgc accacgtcga gcgcgtggcc gttggggatg gcggcgtcca tgacgagcgc
     1561 gatgtcctcc ttggcctgcg ggtaggtgga cacgggccgc acctgcaggg cgtcaaccgg
     1621 gacggccgcg aacaacgcgt ccaggtccag ctcgaaggcc acggtccgcg gcggcaggtc
     1681 caggccggga aggacagccg ggtggagttc gcccgcgtac ccgacgacgg cgccggccac
     1741 caccagctcc gcgcaccggc cggggtgcca cggggtgcga tcgccctgcc ggctggcgat
     1801 gggcaccccg accgtccggc ccgccagttg cgcgagctcg acagcgtccg cccagtcgaa
     1861 ttcccgcgca ccgccgaggg cgccccccgg ggcgggcggg cctgccatca tcgccgcaac
     1921 gtgcagcggc tgggccggga cggcggcgtg gatcgcggcg agggtcccgg ggtccgggag
     1981 ctgcgccacg ggaggcagcg gcgaggaggc cacgtccgcg ccctcggtga cgaggccgat
     2041 ctccatgatc gcgaggtcat tgccgcgccc cacgttccgg cgcgccacct cgatgagcga
     2101 ggcgagcagc gtggaccgca tgaagggcgc gtcgtcggcg atcgggttga ccagccggac
     2161 catccgccgg cgggggtcgt ccgcggggac gtggagacgg tcgtggacgt cacccacgaa
     2221 ggggtacgac agcacctcgt tcagcccgtg ctcggcgagc gagcgcatca ccgcccggcg
     2281 ctgccgctgc cgatccgtca ggccacggcc cgcgggcgcg gccggcacca cggaggggat
     2341 ctcgtcgtag ccacgcagcc gcgcgacctc ctcgaccagg tcctccggga tcgcgaggtc
     2401 agtgcgccag ctcggtggtg tcacggtgag ggtgtcgccg tcgcgctcca cgccggcgcc
     2461 cagcatcgtg agggtcgcga cgacgtcctc gaccgcgtag tccactccca ccacccgtgc
     2521 ggggagccgc ccgtccatca ccacgggacg cggcggggca gtggtgtcca catcggtgac
     2581 gacgt
//
LOCUS       DASRKH010000260         2106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_23510, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000260 DASRKH010000000
VERSION     DASRKH010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23510"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="VFC06_04210"
     CDS             <1..834
                     /locus_tag="VFC06_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020013932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HZL81129.1"
                     /translation="VEQAQDALEDEDAELLRGVVNLGDTLAREVMVPRTDMVTIASGT
                     PLRKAMLLFMRSGFSRVPVIGDGTDDVRGLLYLKDVVRATWDHQDALETDVDDLMREP
                     VFLPESVPADDLLRRMQDEVFHMAIVIDEFGGVAGLVTIEDALEEIVGELTDEHDRAE
                     PQVVDLGGGRFRVPARLAIDELGELFDIEVEDDDVDTAAGLLTKALGKVPIPGSTAAA
                     HGLTLTAERAEGRRRRLTWLLAERTPVPNAGEDDGEASPEGRNRSSGRSNGRRRATSD
                     D"
     gene            827..1732
                     /gene="era"
                     /locus_tag="VFC06_04215"
     CDS             827..1732
                     /gene="era"
                     /locus_tag="VFC06_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HZL81130.1"
                     /translation="MTEFRSGFAAFVGRPNAGKSTLMNALVGEKVAIMSSRPQTTRRA
                     IRGIVNRDDAQLILVDTPGLHRPRTLLGERLNDVVREAFAEVDVIGFCLPADEKIGPG
                     DKWIAQELSQARAPVVAIVTKVDKVSRDRVAAHLVAVQELGDFVDIVPVSSVEDIQVD
                     VLTDVLVGHLPVGPVLYPDGEVTDEPEDVMVAELIREAALEGVHDELPHSIAVMVEEM
                     VEREQAADDPRTPVLIIRAQLYVERDSQKGIIIGKGGERLKAVGAEARAGIERLLGRR
                     VYLDLHIKIAKEWQRDPKQLGRMGF"
BASE COUNT          371 a          643 c          739 g          353 t
ORIGIN      
        1 gtcgagcagg cccaagacgc gctcgaggac gaagacgctg agcttcttcg cggcgtcgtg
       61 aacctgggcg acacccttgc gcgtgaggtg atggtgccgc gcaccgacat ggtgacgatc
      121 gcgtccggca ctccgctccg aaaggcgatg ttgctgttca tgaggtcggg attctcccgc
      181 gtgcccgtga tcggcgacgg cacggacgac gtgcgggggt tgctgtacct gaaggacgtg
      241 gtgcgtgcga cgtgggacca ccaagacgcg ctcgagaccg acgtggacga cctgatgcgc
      301 gagccggtgt tcctgcccga atccgtgcca gccgatgact tgctgcgccg catgcaggac
      361 gaggtgtttc acatggccat cgtgatcgat gagttcgggg gcgtcgccgg cctcgtcacg
      421 atcgaggacg ccctcgagga gatcgtcggc gagctgaccg acgaacacga tcgtgccgaa
      481 ccccaggtcg tggacctcgg cggcggacgt tttcgggtgc cggcgcgact tgcgatcgac
      541 gagctgggcg agctgttcga cattgaggtc gaggacgacg acgtggacac ggcggcgggg
      601 ctgctcacca aggcgctcgg caaggtgccg attcccggat cgaccgcagc ggcccacgga
      661 ttgaccctca cggccgaacg cgcggagggg cgcaggcgta ggctcacgtg gctactggcc
      721 gagcgcacgc ccgtgccaaa cgctggcgaa gatgacggcg aagcctcccc cgagggccgg
      781 aatcgctcgt ctggacgttc caatggaaga agaagggcca cttcagatga ctgagtttcg
      841 ttccggcttt gccgcgtttg tggggcgacc caacgccggc aagtcgaccc tcatgaacgc
      901 gctcgtgggg gagaaggtcg ccatcatgtc gtcgcgccct cagaccacgc gtcgcgcgat
      961 tcgcggcatc gtgaatcggg acgatgccca attgatcctc gtcgacacgc ctggactgca
     1021 tcggccgcgc acgctgttgg gtgagcggct caacgacgtg gtgcgggagg cgtttgccga
     1081 ggtggacgtc atcggcttct gccttcctgc cgacgagaag atcggtccgg gcgacaagtg
     1141 gatcgcgcaa gaattgagcc aggcgagagc tcccgtggtc gccatcgtca ccaaggtcga
     1201 caaggtgtca cgggatcgcg tggccgcgca cctggtggcg gtgcaggaac tcggcgactt
     1261 cgtcgacatc gttcccgtgt cgtcggtcga agacattcag gtggatgtgc tgacggacgt
     1321 cctcgtgggt cacctcccgg tgggccccgt gctgtacccg gatggcgagg ttacggacga
     1381 acccgaggac gtcatggtcg ccgagctgat tcgcgaggcc gcccttgagg gcgtgcacga
     1441 cgagctgcct cactcgattg ccgtgatggt cgaagagatg gtggagcgcg aacaggccgc
     1501 ggacgacccc cggacgccag tgctcatcat tcgcgcccag ttatacgtcg agcgggacag
     1561 ccagaagggc atcatcatcg gcaagggagg cgaacgcctc aaggccgtgg gagccgaggc
     1621 gcgcgcggga atcgagcgtt tgcttggccg cagggtctat ttagacctgc acatcaagat
     1681 tgccaaggag tggcagcgcg atcccaagca gttggggcgc atggggttct agaggcgtca
     1741 ggtcatcgcc ggtcgccgtt tcggtctatt gaagcgactc ggcgcagcct tcgaggtgtg
     1801 ttgcagcgac tgcgagtagg agtccttcca cgcccgagac ctcggccccc caagccggga
     1861 tgtgggccca gagaggcgtc ctcatcgttg ctagggttgt ggtgtcatcg tggtggggcg
     1921 cacctccatc cgcggcgcag tcgccgccaa cgatcccggg tcttgctacg aaggcggaga
     1981 ctgccgcgag cccgccatct ccggacggac cagctcgaca ccacagaagg gcatgagtga
     2041 tgagcaaccg cgaggcgccg caaccgagtc cgtcgaggct gacccccttc agcaaccacc
     2101 cgatgg
//
LOCUS       DASRKH010000261         3075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_31532, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000261 DASRKH010000000
VERSION     DASRKH010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31532"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="VFC06_04220"
     CDS             <1..740
                     /locus_tag="VFC06_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012877950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HZL81131.1"
                     /translation="AHGVSVAGRAVVSGGAYGIDAAAHRAALANDAVTIAVMAGGVDR
                     LYPVGNDDLLKRIASAGAVVSEVPPGFAPHRSRFLSRNRLIACASATVVVEAAARSGA
                     LSTARHAAELLRPVGAVPGPVTSASSTGCHALVREGAAVLVTGADDVLELAAPLTEER
                     GTPDRSEVTDVQGRLRFDFGSLGERKMFDVLTRRGKAVETAAASAGLSLDEARAALGA
                     LESAGLVERYRSGWRRSEVRISVKKGT"
     gene            832..1737
                     /locus_tag="VFC06_04225"
     CDS             832..1737
                     /locus_tag="VFC06_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HZL81132.1"
                     /translation="MAADAGTVRERYAAALRHGYGASTHTVRAYLGDLDDFANHLELD
                     DDSTWGGVQLADIRSWLAHMAEDGKSRSTLARRGAAIRTFFAWAHRTGLIDHNPALRL
                     ASARPASTLPEVLGVVDMSLLLDVAEVRADSGDPLDLRDWAMAELLYATGIRVGELTG
                     VDVADVDLAELTVRVVGKGDKERVVPFGIPAMRALVAWLDVGRAVCAPSDARALFVGR
                     RGGRIDQRQVRDVIHTLAGLAGVPDIAPHALRHTAATHLLQGGSDLRTVQEVLGHASL
                     TTTQRYTHVTADRLRSAYHLAHPRA"
     gene            1749..2549
                     /locus_tag="VFC06_04230"
     CDS             1749..2549
                     /locus_tag="VFC06_04230"
                     /inference="COORDINATES: protein motif:HMM:NF005413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HZL81133.1"
                     /translation="MTTYEAELDTVPVPTLLAATWETLATGTVEQQRDARSHLIMQYA
                     PLVTQVATRMIGRLPDSVELADLVSYGMFGLIDAIHKFDVGRGFKFETYAATRVRGSI
                     IDELRAADWVPRSVRTKARAVETATRSLEQHLLRKVTDEDVAQELGWSTAEVRTVRAQ
                     VALSHIGALDSFGMGDDTTMIESVAALAVPGAGNSVEVRETHALLATAVQGVRDREQQ
                     VLQLYYYENLTLAQIGQILGVTESRVSQIHSGAVKKLRESLEQTGAFS"
     gene            complement(2546..>3075)
                     /locus_tag="VFC06_04235"
     CDS             complement(2546..>3075)
                     /locus_tag="VFC06_04235"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016996506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZL81134.1"
                     /translation="RALTPRRKPLALMAAAACVALVSGTPAHAAATVVCASPVEPFSV
                     SQLASLPAQNWLPGHRRIDLATSVGRIVNSPAAGEVIYAGIVVNRPVVSVRHTGGFVS
                     SLEPVDATVRVGDLVDVGGALGTVSVATGHCAPATCVHWGLRRDGRYVNPLDYLLGYG
                     QVRLLALDGWAENAP"
BASE COUNT          539 a          998 c         1043 g          495 t
ORIGIN      
        1 tcgcacatgg agtgtcggtg gcgggtcgcg ccgtagtctc cggaggcgcc tacggcatcg
       61 atgcggcagc gcatcgggcg gcgctcgcca acgacgcggt gaccatcgcc gtgatggcgg
      121 gaggggtgga ccgcctgtac cccgtcggca acgacgacct cctgaagagg atcgcgtccg
      181 cgggggcagt cgtgagcgag gtgccacccg gattcgcgcc gcaccgctcg cgatttctga
      241 gccgaaatag gctgatcgcc tgcgcgagcg cgactgtggt ggtcgaggcg gcggcgcggt
      301 ccggggcgct gagcacggca cgccatgcgg ccgagttact gaggcccgtc ggcgccgttc
      361 cggggccagt cacctccgcg tcgtccacgg gttgccatgc cctggtgagg gaaggcgcgg
      421 ccgtccttgt cacgggtgcc gacgacgtac tcgagcttgc ggctcccttg acggaggagc
      481 gtggcacgcc ggaccgctcc gaagtgacgg acgtgcaagg ccgacttcga ttcgattttg
      541 gctcgcttgg agagcgcaag atgttcgacg tgctgacgcg aaggggcaag gccgtggaga
      601 ccgccgcagc ctccgcgggg ctctcccttg acgaggcgcg tgccgcgctt ggggcacttg
      661 aatccgcggg cctcgtggag cgataccgct ccggttggcg tcgaagtgag gtccgcatat
      721 ctgtcaaaaa aggaacataa cggtgcaaaa ggtcacaaaa tagggttact gatgtgacaa
      781 atgtgactca cggggttgat ttgacgggag tgacggtgcc agagttgagc catggcagcc
      841 gatgcgggca cggtgaggga gcggtacgcc gctgccctgc gccatggcta cggcgcctcg
      901 acccacacgg tccgggctta cctcggtgat ctggacgact tcgcgaatca cctcgaattg
      961 gacgacgact ccacgtgggg tggcgttcaa cttgctgaca ttcgttcgtg gctcgctcac
     1021 atggcggaag acggcaagag caggagcacc ctagcgaggc gaggcgcggc aatccgcacg
     1081 ttcttcgcct gggcgcatcg gaccggcctc attgaccaca atccagccct gcgtcttgcc
     1141 tcggcccggc ccgccagcac gcttcccgag gttctcggag tggttgacat gagcctcttg
     1201 ctcgacgtcg ccgaggtgcg tgccgatagc ggagacccgc ttgacttgcg ggattgggca
     1261 atggccgaac tgctctatgc gaccggcatc cgggtggggg agctcacggg cgttgacgtc
     1321 gcagacgtcg acctggccga actcaccgtc agggtcgttg gcaagggcga caaggaacgc
     1381 gtggtcccct tcggtatccc agcaatgcgc gcactcgttg catggctcga cgtgggccgc
     1441 gcggtgtgcg cacccagcga cgcccgtgcc ctcttcgtcg ggcgacgcgg cggacgcatc
     1501 gaccaacgcc aggtgcgcga cgtgatccac acgctcgccg ggcttgccgg cgtccccgac
     1561 atcgcacccc acgcgctgcg ccacacggcc gcgacccacc tcctgcaggg ggggtcggat
     1621 ctgcgcacgg tgcaagaggt gctcggccac gcgtcgctca cgaccacgca gcgctatacc
     1681 cacgtgacag ctgatcgttt gcgtagtgcc tatcaccttg ctcatccgag ggcctgaggg
     1741 gaaacaccat gaccacgtat gaagctgagc tcgacacggt tcccgtgccc acgctgctcg
     1801 cggccacctg ggagacgctc gcaaccggga cggtggagca gcagcgcgac gctcgctctc
     1861 acctgatcat gcagtacgcg ccgcttgtga cacaggtcgc cacccgcatg atcggcagac
     1921 tgcccgattc ggtggaactc gccgacctcg tctcctacgg aatgttcggc ctgatcgacg
     1981 cgatccataa gtttgacgtg ggcaggggct tcaagttcga aacctacgcg gctacccgcg
     2041 tgcgcggctc catcatcgac gagcttcgcg cggccgattg ggtgccccgc tcggtgcgca
     2101 cgaaggcgcg cgctgtggag accgccactc gcagtctcga gcagcacctt ctgcgcaagg
     2161 taactgacga agacgtggcg caggagctcg gctggtcaac agcggaggtg cgcacagtgc
     2221 gcgctcaggt cgcgctcagc cacatcggcg cgctggactc gtttggcatg ggcgatgaca
     2281 ccaccatgat tgagagtgtc gcggcgctag cagtgccggg cgcggggaac tcggtggagg
     2341 tgcgcgagac acacgcgttg ctggccaccg ccgtccaggg tgtgcgtgac cgcgagcagc
     2401 aagtgctcca gctctactac tacgagaacc tcacgcttgc ccagatcggc cagattctcg
     2461 gagtgacaga gtcacgggtg tcccagattc actcgggcgc cgtgaagaaa cttcgcgaaa
     2521 gccttgagca aaccggggcg ttctcctaag gggcgttctc agcccacccg tcgagcgcaa
     2581 gcagccgcac ctgtccgtag ccgagtagat agtcgagcgg attgacgtac cggccatcac
     2641 gccgcagccc ccaatgaacg caagtcgcgg gagcgcaatg gccagtggcg accgacactg
     2701 ttcccagggc cccacccacg tccacgaggt ctccgacgcg caccgtcgcg tcaacaggtt
     2761 cgagggacga cacgaagccc cccgtatgtc gcaccgacac cacgggcctg ttgacgacaa
     2821 tgcccgcgta gatgacctcg ccagctgcgg gggagttcac gatgcgtccc accgacgtcg
     2881 cgagatcgat cctgcggtga cccggcagcc agttctgcgc aggcaacgat gcgagctgcg
     2941 agaccgaaaa cggctcgacg ggggaggcac acaccactgt cgctgctgca tgggcggggg
     3001 tgcccgacac gagcgcgacg cacgccgccg ccgccatgag agcgaggggc tttcggcgtg
     3061 gggtcagggc gcgca
//
LOCUS       DASRKH010000262         3186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_63290, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000262 DASRKH010000000
VERSION     DASRKH010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63290"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="VFC06_04240"
     CDS             <1..638
                     /locus_tag="VFC06_04240"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF019174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HZL81135.1"
                     /translation="IPVMENGTRVGAVLLCRDVSDLRRRERELITKDATIREIHHRVK
                     NNLQTVAALLRLQSRRVEAPEAKEALSEAMRRVATIAMVHETLSGTLDELVHFDDLVA
                     RAARMAADVASPDVQVKIVREGDFGMIPAQFASSLALILTELVTNAVEHGFEGREHGS
                     ITIFAERDGATLHARITDDGKGIAVDPVDSAHRLSARSSPTNSTERSYGGP"
     gene            complement(704..958)
                     /locus_tag="VFC06_04245"
     CDS             complement(704..958)
                     /locus_tag="VFC06_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009772809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HZL81136.1"
                     /translation="MDWRDRAGCLDVDDPELFFPVGNTGPALVQIEKAKTVCNSCTVR
                     EVCLQWAMEHNQDSGVWGGLSEDERRSLKRRAARARRAAK"
     gene            1116..1511
                     /locus_tag="VFC06_04250"
     CDS             1116..1511
                     /locus_tag="VFC06_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81137.1"
                     /translation="MHKPRAFSAVLDLLALAILILETLATAFLAVGYGIYALLDSRDP
                     GMAAALGGVMAVFAVGVGAFSWGFAKRRRFALSGALAWQLMQASVGVWLITAWPLVGT
                     ALILLAAVVTVAVVRRQAAYGKPGAAEPS"
     gene            complement(1478..>3186)
                     /locus_tag="VFC06_04255"
     CDS             complement(1478..>3186)
                     /locus_tag="VFC06_04255"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HZL81138.1"
                     /translation="HPLLHGARLRDGPGPHTAPPAGPHLAAIAGPDAGVVIEIGGRVA
                     IGSAPGRDQIRDDALDPTHLVVKPTAPGALACRDAGTMNGTGWWRYDGQRWWWSGRRR
                     RFTARQGDILTVGNTALQVRGNVSASRHGNPRQLLFAAWRLLAPLPWWPSPSWAGLPD
                     PTVAAGWTGDVRITGPGAREAARAVILARGRRPPSPPPFDEEWLRWLPAALPSDGPVR
                     CGSAAPAPMEVTIEAHADHCRLSGSPDTAERLPMAVSERTADALARTLAGAAAVPWPH
                     AVRWADVDRPHLHTPDGADLSVALGAACSPDSEPWIVVLNGRTPHLLAAGARAAGAST
                     LLATLVGGLAHHYDSRRLRIVLIGSGTDGPLAPCASLPHITSATATAGGDEALRVLSA
                     VAKQASQRRDALQASGAPNWRTWEASGNAPGRLLVVVDDFDLATGRSRTAAAAIEALT
                     TAPVFVGIHVALATHRPAGAVTPALRSACHHTVALRSASESDSRGVIGVPDAAALDAA
                     PGQAVVNVGGKRTTVQVALPFADASPRVRRADAGLAPALHLADAVVMRARQEGSAAPG
                     LP"
BASE COUNT          521 a         1077 c         1108 g          480 t
ORIGIN      
        1 ccattccggt gatggagaac ggcacccggg tgggagccgt gctgttgtgc cgtgacgtga
       61 gtgacttgcg acggcgcgaa cgcgagctca tcacgaagga cgcgacgatt cgcgagattc
      121 accaccgcgt gaagaacaac ctccagacgg tcgcggcctt gctgaggcta cagtcccgcc
      181 gagtcgaggc gcccgaggcg aaggaagcgc tgtcggaggc gatgcgccgc gtggcgacca
      241 ttgcgatggt gcacgagacg ctgtcgggca ccctggatga gctcgtgcac ttcgacgacc
      301 tggtggcacg tgccgcgcgc atggcggcag atgttgcctc gcccgacgtt caggtcaaga
      361 ttgtgcgcga aggcgacttc ggcatgatcc cggcgcaatt cgcgtcgtcc cttgccctga
      421 tcctcaccga actggtgacc aacgctgtcg agcacggttt cgaagggcgc gagcacggct
      481 cgatcacgat cttcgccgag cgagacggcg ccacgctgca cgcacggatc accgatgacg
      541 gcaagggcat tgcggtagac ccagtggact cggctcacag attgtccgca cgctcgtcac
      601 caacgaactc cacggagaga tcttatggcg gtccgtagcg ggcggctcga gcgtcgacct
      661 cacggtcacc gtggacggcg actagggtcg gcttgcggtg cggctacttg gcagcgcggc
      721 gagcgcgggc cgcgcgtctc ttgagcgagc ggcgctcgtc ttccgacagg ccaccccaca
      781 cgccagagtc ctggttgtgc tccatggccc actgcaggca cacttcacgg accgtgcacg
      841 agttgcatac ggtcttggcc ttctcgatct gcacgagcgc cggccctgta ttccccactg
      901 ggaaaaacag ctccggatcg tcgacgtcga ggcaccctgc gcggtcgcgc caatccatca
      961 agactcctca caaagaaaaa aggcggcgaa ctacgctgac gtagattcgc cgcgccgtcg
     1021 cggatcactg ggcccagttt cgcattgtta acaacagatg acaagggtag aaacctgtaa
     1081 cgtccgtcac aattccatga taagggtggg agaccatgca caaaccacgg gcattttcgg
     1141 cagtactcga tctcctcgcg ctcgcgatct tgatcctgga gacactggcg accgcgttcc
     1201 tggcggtcgg ctacggaata tatgccctgc tagatagccg ggatccgggg atggccgcgg
     1261 cgctcggtgg cgtgatggcc gtcttcgctg tgggggtggg agccttctcg tggggcttcg
     1321 ccaagcgccg ccggttcgct ctgagcggag cgctcgcttg gcaactcatg caagcgagcg
     1381 tcggcgtgtg gctgatcacg gcgtggccgc tggtggggac agctttgatc ctcttggcgg
     1441 ccgtggtcac ggtcgcggtg gtgcgcaggc aggccgccta cggcaagcca ggagcggcag
     1501 agccctcctg acgcgcccgc atcacgaccg cgtccgccag gtggagcgca ggcgcgagcc
     1561 cggcatccgc acggcgcact cgcggggacg cgtcggcgaa aggaagcgcc acctgaaccg
     1621 tcgtccgttt ccccccgacg ttcacgaccg cctgtcccgg agcggcgtcc agcgccgcgg
     1681 cgtcgggcac gccgatcacg ccacgcgagt cggattccga cgctgagcgc agtgccacgg
     1741 tgtggtggca tgccgacctc agcgctggcg tcacggctcc agcgggacgg tgcgtcgcca
     1801 gtgccacgtg gatgcccaca aacaccggcg cggtcgtcaa cgcctcgatc gcggcggcgg
     1861 ctgtgcgtga gcgacccgtg gcgagatcga agtcgtcgac caccaccagg agccggcccg
     1921 gggcgttgcc cgatgcctcc cacgttcgcc aattgggcgc ccccgaagcc tggagggcgt
     1981 cccggcgctg actcgcctgc ttcgccacgg cgctcaagac gcgcagggcc tcgtcgccgc
     2041 cagcggtcgc cgtggcactc gtgatgtggg gcaggctcgc acatggcgcg agaggcccgt
     2101 ccgttccgga tccgatcagc acgatccgaa ggcgacgcga gtcgtagtgg tgggccaatc
     2161 cgcccaccag ggtggcgagc agcgtcgagg cgccggcggc gcgcgcgccc gcggccaaca
     2221 gatgcggggt gcgcccattg agcacgacaa tccacggctc gctatcgggc gaacaggcgg
     2281 cgcccaacgc gacgctcagg tcggccccat ccggcgtgtg caggtggggc ctgtcgacat
     2341 cggcccaccg caccgcatgc ggccagggga ccgccgccgc gccggcgagc gtccgagcca
     2401 gcgcatcggc ggtgcgctct gacaccgcca tcggcaagcg ctctgcggtg tcgggcgacc
     2461 ctgagagacg acagtggtcg gcgtgcgcct cgatagtcac ttccatgggt gctggcgcgg
     2521 ccgaaccgca ccgcacggga ccgtctgagg gaagggctgc tggcaaccac cggagccact
     2581 cctcgtcgaa gggcggaggt gacgggggac gtcttccgcg cgccaggatc accgcgcgtg
     2641 ccgcctcccg ggcgccgggc ccagtaatcc ggacgtcacc ggtccaccca gccgcgaccg
     2701 tcgggtctgg tagcccggcc caagaaggag agggccacca gggcagtggt gccagcagcc
     2761 gccatgcagc gaacaggagc tgccgggggt tcccgtgacg cgaggcactg acgttgccgc
     2821 gcacctgtag ggcggtgttt ccgaccgtca ggatgtcgcc ctgccgggcg gtgaaacgcc
     2881 gccgtcgccc tgaccaccac cagcgctgcc cgtcgtagcg ccaccacccg gtgccgttca
     2941 ttgtgcccgc atctctgcag gcgagcgcgc caggcgccgt cggcttgacg acaaggtgag
     3001 tcgggtcaag ggcatcgtcc cgaatctggt cgcgacccgg cgccgaaccg atcgcgacac
     3061 gacccccgat ttcgatgacg acccccgcgt ccggacctgc gatggcggcc agatgcgggc
     3121 cggcaggggg agcggtgtgc ggcccgggac cgtcgcggag gcgtgctccg tgaagtagcg
     3181 ggtgga
//
LOCUS       DASRKH010000263         3213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_43237, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000263 DASRKH010000000
VERSION     DASRKH010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43237"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            324..1853
                     /locus_tag="VFC06_04260"
     CDS             324..1853
                     /locus_tag="VFC06_04260"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HZL81139.1"
                     /translation="MTAAIDTPEAAAQDKQHARGKKTARERVELLLDEGSFVEFDALT
                     RHRATAFGLENNRPFGDGVVTGYGTVDGRQVAVYSQDFTVFGGSLGEIHGHKITKVQD
                     FALRTGVPLIGISDGGGARIQEGVAALTQFAEIFRRNVAASGVIPQISIILGPSAGGA
                     VYSPALTDFIVMADGTSQMFITGPDVIKSVTGEEVTFEDLGGGLTHNVKSGVAHYLAD
                     NEDDAIEYVQHLLQFLPQNNLSDPTVWDQEADLELSEDDLALDALVPDSDNQPYDMNT
                     LIETVVDEGDFLEVQPLFAPNIVVGFGHVEGHSVGIVANQPLSMAGTLDINAAEKAAR
                     FVRTCDAFNIPVLTFVDVPGFLPGIGQEHQGIIRRGAKLIYAYAEATVPLITVITRKA
                     YGGAYIVMASKQLGADINLAWPTAQIAVMGASGAVNILSRRALAGVKEQGGDVEAERQ
                     ALIDDYDDRLVNPYNAADKGYVDAVIEPSQTRAQIVRGLRALRTKRAALPPKKHGNIP
                     L"
     gene            1850..2095
                     /locus_tag="VFC06_04265"
     CDS             1850..2095
                     /locus_tag="VFC06_04265"
                     /inference="COORDINATES: protein motif:HMM:NF025192.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit epsilon"
                     /protein_id="HZL81140.1"
                     /translation="MSEQDDLVAAASSMRVVRGTLEDDELAALVAGMVAMASAAEDDD
                     DEPGSPTSAWMDRARTMRGVQVGVLGRGSGAWRHSLR"
     gene            2092..2721
                     /locus_tag="VFC06_04270"
     CDS             2092..2721
                     /locus_tag="VFC06_04270"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HZL81141.1"
                     /translation="MRRLHARLHPRDRWVVVAIVAIPTLVATPWAVVRVSTASSTHPA
                     EASFARADAAVVLGARVYADGRPSRFLRERIEVGVRLYLDGTVDRLIMSGDGDDSSGF
                     GEPSVMRQVAEDMGVPPEAIVEDPLGVDTYSSCVNARDTFGATSVIMVSQEFHVPRAV
                     WVCDQIGLDAQGAYPPQRLTKSTAVGHVREVAADAKAMIDVMRGRIPQG"
     gene            2795..3010
                     /locus_tag="VFC06_04275"
     CDS             2795..3010
                     /locus_tag="VFC06_04275"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HZL81142.1"
                     /translation="MSALSPGSAAHVRSVSTESGVVLRLREMGLGPGTLLRLTGRGPS
                     GSRIVSVGAARLAIDATTCSLIEVDPT"
     gene            3007..>3213
                     /locus_tag="VFC06_04280"
     CDS             3007..>3213
                     /locus_tag="VFC06_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HZL81143.1"
                     /translation="MNQAVRTARILLVGNPNVGKSTLFNALTKARQRVVNAPGTTVSL
                     TAGTWRIQGDDVSLVDLPGTYSLIA"
BASE COUNT          545 a         1091 c         1053 g          524 t
ORIGIN      
        1 gttctccagc gcgatgtgct cagtgatgag gtcctttaca gggaccttcc cttccatgcc
       61 tcgcgtcttt gcgagaggcg gagatactcg gtgcccgttt gagcccgttt tcgttgacaa
      121 tcggcaatcc agacccgcaa gcgggcttag tcaaggggta aacggacttg atcaggcctt
      181 ttttcgaatg ccctcctccc gagtggctgt ggcaaggggc cagcccccgg ctaggctgtg
      241 tgcgagttga ccgagggagc actgtggccg acacaccaaa cgccaagtcc accgcgtccg
      301 cggtcgatgc gctgcgcgac aagatgaccg ccgctatcga tacgcccgag gctgcggcgc
      361 aagacaagca gcacgcacgc ggcaagaaga ccgcgcgcga gcgcgtcgag ctgttgctgg
      421 acgagggctc gttcgtcgag ttcgacgccc tcacgcgcca ccgcgccacg gcgtttggcc
      481 tggagaacaa ccgtccgttc ggcgacggcg tcgtcaccgg gtacggaacc gtggacgggc
      541 gccaggtagc cgtctattcg caggacttca ccgtcttcgg cggctcgctg ggcgagattc
      601 acggccacaa gatcaccaag gtgcaggact tcgcactgcg cacgggcgtg ccgctgatcg
      661 gcatctccga cggtggcgga gcgcgcattc aggaaggcgt cgcagccctc actcagttcg
      721 ccgagatctt ccgccgcaac gtggccgcat cgggcgtcat cccgcagatc tccatcatct
      781 tgggcccaag cgctggcggc gccgtctact ccccggcgct gaccgatttc atcgtcatgg
      841 ccgacggcac gtcccagatg ttcatcaccg gtcccgacgt catcaagtcg gtgacaggcg
      901 aagaggtgac gtttgaggac ctcggcggcg gtcttacgca caacgtgaag tcgggcgtgg
      961 cgcactacct ggccgacaac gaggacgacg cgatcgagta cgtgcagcac ttgctgcagt
     1021 tccttccgca gaacaacctg tccgatccca ccgtgtggga ccaagaggcg gacctggagc
     1081 tctccgagga cgacctcgca cttgacgcct tggtgcccga cagcgacaac cagccgtacg
     1141 acatgaatac tctcatcgag accgtggtcg acgagggaga cttcctcgag gtgcagcccc
     1201 tgttcgcccc caacatcgtg gtgggttttg gccacgtcga gggccactcc gtcggcattg
     1261 tggcgaacca gccgctgtcc atggcgggca cgctcgacat caatgccgcg gagaaggccg
     1321 cgcggtttgt gcgcacgtgc gacgcgttca acatcccggt gctcacgttt gtggacgtgc
     1381 ccggctttct gccgggcatc ggccaggagc accaaggcat catccgccgc ggcgccaagc
     1441 tcatctacgc gtacgccgag gccacggtgc cgctcatcac cgtcatcacc cgcaaggcct
     1501 acggcggcgc gtacatcgtc atggcgtcca agcagttggg tgccgacatc aacctcgcct
     1561 ggcccaccgc acagatcgcc gtcatgggcg cgagcggcgc ggtcaacatc ctgtcccgcc
     1621 gcgcactcgc cggggtcaag gagcagggag gcgacgtcga ggcggagcgc caggcgctca
     1681 tcgacgacta cgacgaccgc ctcgtcaatc cctacaacgc cgccgacaag ggttacgtcg
     1741 acgcagtgat cgagccctcg cagacgcgag cgcagatcgt gcgcgggctg cgtgcgctgc
     1801 gcaccaagcg ggcggcactg cctcccaaga agcacgggaa catcccgctg tgagcgagca
     1861 ggacgacctc gtcgcggccg catcttcgat gcgagtggtg cggggcaccc tggaagacga
     1921 cgagttggcc gccctcgtgg cgggcatggt cgcgatggcg agcgccgcag aagacgacga
     1981 cgacgagccc ggctcgccca cgtccgcgtg gatggatcgc gcgcggacga tgcgcggagt
     2041 ccaggtggga gtgctcggcc gcggctcggg cgcctggcgg cactcgctgc gatgaggcgc
     2101 cttcacgccc ggctgcatcc gcgtgaccgg tgggttgtcg tcgcgatcgt cgccataccg
     2161 acgttggtgg caacgccgtg ggccgtcgtg cgcgtctcga ccgcctcatc gacgcaccca
     2221 gcggaggcct ccttcgcccg cgccgacgcg gcagtggtgc tgggtgcccg cgtgtacgcg
     2281 gacgggcgac cctcgaggtt cctgcgcgag cgcatcgagg tgggggtgcg tctctacctg
     2341 gacggcaccg tcgacaggct catcatgagc ggcgacggcg acgacagttc cggcttcggg
     2401 gagccgagcg tcatgcggca ggtggccgag gacatgggag tgcctcccga ggcgatcgtg
     2461 gaggacccgc tcggcgtcga cacctattcg tcctgcgtca acgcccgcga cacgttcggc
     2521 gccaccagcg tgatcatggt cagccaggag ttccacgtcc cgcgtgcggt ctgggtgtgc
     2581 gaccagatcg gcttggacgc tcaaggcgcg tacccgccgc aacgcctcac caagtccacc
     2641 gctgtgggtc atgtgcgcga ggtcgcggcc gatgccaagg ccatgatcga cgtgatgcgc
     2701 ggccgcatcc cacaagggtg accgcaccgg ccccaaaacc gggacgtaag taattaggca
     2761 agccttacct tgcatatatc ctgagtgcat gctgttgagc gcgctctccc ccggttccgc
     2821 cgcccacgtg cgttcggtga gcacggagtc cggggtcgtc ctgcgcctgc gagagatggg
     2881 gttggggcca ggcactctac tgcgcctcac cgggagaggc ccttccggat cgcgcattgt
     2941 ctccgtcggt gcggcacgcc tggcgatcga cgccaccacg tgctcgctca tcgaagtgga
     3001 tcccacgtga accaagccgt gcgcaccgcg agaatcctgt tggtcggcaa cccgaacgtc
     3061 ggcaagtcga cgctgttcaa cgccctgacc aaagcccgcc aacgcgtcgt caacgccccg
     3121 ggcacgaccg tctcgttgac cgccggaacc tggcgcatcc agggcgacga cgtgtcgctc
     3181 gtcgacctgc ccggcacgta cagcctcatc gcc
//
LOCUS       DASRKH010000264         2045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_27695, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000264 DASRKH010000000
VERSION     DASRKH010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27695"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..1490)
                     /locus_tag="VFC06_04285"
     CDS             complement(99..1490)
                     /locus_tag="VFC06_04285"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HZL81144.1"
                     /translation="MTGADEKLVAAGVDNYLLHESRQQPNWPDKDALHRVVDRLKMVP
                     PLVFAGEADVLRSHLAAAGRGEAFVLQGGDCAETFSEATADRIRNKIKTILQMAVILT
                     YGASTPVVKIGRMAGQYAKPRSSDSETRDGMTLPAYRGDIVNHSSFVPELRVPNPERL
                     VDAYHVSASTLNLIRAFTTGGFADLRRVHQWNRGFAGNRAYAAYDKVAGEIDRAVRFM
                     EAAGGDFEALKTVEFFSSHEALLLDYERALTRIDSRSGLPYDCSAHFLWIGERTRQLD
                     GAHVEFMSKIHNPIGIKLGPTSTAADALALAERLNPDNIPGRITFVARMGADRVRDVL
                     PGIVGGVRDAGASVTWLSDPMHGNTFTSESGFKTRRFETILDEVKGFFEVHRGLDTVP
                     GGLHVELTGDDVTEVMGGTAEIDDEGLARRYETKVDPRLNHEQSLEMAFLVSELLSQR
                     LKPRLEDLWGDHS"
     gene            complement(1487..>2045)
                     /locus_tag="VFC06_04290"
     CDS             complement(1487..>2045)
                     /locus_tag="VFC06_04290"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866584.1"
                     /note="catalyzes the formation of fructose
                     1,6-bisphosphate from fructose 6-phosphate and
                     diphosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyrophosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HZL81145.1"
                     /translation="DTREWVPDIGLSRDRWEIHAVYVPEQSIDLDAEADRLRAVMDSV
                     GNVNIFLSEGAGVPEIIKEIEEAGGKVKKDPFGHVKLDTINPGNWFAQQFADRIGAEK
                     VMVQKSGYFSRSARANAEDLRLIKSMTDYAVECALRGEPGVVGHDEEDGGRLKCIAFP
                     RIAGHKAFDTSTPWYVDLLADIGQS"
BASE COUNT          332 a          680 c          672 g          361 t
ORIGIN      
        1 ctgctggaga tcgctgcggc aaacccgcag cttccctacg gcctgccccc gacgagcgtc
       61 gggcttcgat gacctaggac cgttgcgcgt cgctcgagct agctgtggtc tccccagaga
      121 tcctcgagcc gaggcttcaa ccgttgcgac aacagctcgg acaccaggaa cgccatctcg
      181 agagactgct cgtggttgag gcgggggtcc accttggtct cgtagcgtcg ggccaggccc
      241 tcgtcgtcga tctccgcagt tccacccatg acctcggtca cgtcgtcgcc cgtgagttcc
      301 acgtgcaggc cgccgggaac ggtgtcgagc ccacggtgca cctcgaagaa gcccttgacc
      361 tcgtcgagga tcgtctcaaa ccggcgggtc ttgaagccgg actccgacgt gaaggtgttc
      421 ccgtgcatcg ggtcgctgag ccacgtgacg cttgcgcccg cgtcgcgcac acctcccact
      481 atccccggaa gcacgtctcg caccctgtcc gcgcccatgc gggccacaaa cgtgatgcgg
      541 ccgggaatgt tgtcggggtt gagccgctcc gcaagggcca gggcgtcggc cgcggtgctc
      601 gtggggccga gcttgatgcc gatggggttg tgaatcttcg acatgaactc gacgtgcgcg
      661 ccgtccagct gacgggtgcg ctcgccgatc cacaggaagt gagccgagca gtcgtacggc
      721 aagcctgagc gcgagtcgat gcgcgtgagc gcccgctcgt agtccagcag cagcgcctcg
      781 tggctggaga agaactccac ggtcttgagc gcctcgaagt cgccgccggc ggcctccatg
      841 aaacgcacgg cccggtcgat ctcccccgcc accttgtcgt aggcggcgta cgcgcggttc
      901 cccgcaaagc cgcggttcca ctggtggacg cggcgcaggt ccgcgaatcc acccgtggtg
      961 aaggccctaa tcagattgag ggtcgacgcg gacacgtggt acgcgtccac gagccgctcc
     1021 gggttgggca cccgcaactc cggcacaaac gacgagtggt tgacgatgtc gccccggtac
     1081 gcgggcaggg tcattccgtc cctggtctcg gagtcggacg aacggggctt ggcgtattgg
     1141 cccgccatgc gcccgatctt cacgacgggc gtcgaggctc cgtacgtgag gatgaccgcc
     1201 atctgcagga tggtcttgat cttgttgcgg atccgatccg cggtcgcctc agagaaggtc
     1261 tcggcgcagt cccccccttg cagcacgaag gcctcgccac ggcccgccgc cgcgaggtga
     1321 ctgcgcagca cgtcggcctc gcccgcgaac acgagcggcg gcaccatctt gaggcgatcg
     1381 acgacacgat gcagggcgtc cttgtcaggc cagttgggct gctgcctgga ctcgtgcagg
     1441 aggtagttgt cgacgccagc tgcgacgagc ttctcgtcgg cgccggtcat gactgcccga
     1501 tgtctgccag cagatcgacg taccaggggg tggaggtgtc gaaggccttg tggccggcga
     1561 tgcgcgggaa cgcgatgcac ttcaggcgac cgccatcctc ctcgtcgtgt cccaccacgc
     1621 cgggctcgcc gcggagggcg cactccacgg cgtaatcggt catcgacttg atgaggcgca
     1681 gatcctcggc gttggcacgg gctgagcgag agaagtagcc ggacttctgc accatgacct
     1741 tctcggcacc aatgcggtcg gcaaactgct gggcaaacca gttaccaggg ttgatggtgt
     1801 cgagcttgac gtggccgaag gggtccttct tgaccttacc gcctgcttcc tcgatctcct
     1861 tgatgatctc gggcacgccc gcaccctccg acaggaagat gttcacgttg cccacggagt
     1921 ccatgacggc cctcaggcga tcggcttccg cgtcgaggtc gatcgactgc tcgggaacgt
     1981 acacggcatg aatctcccag cggtcgcgcg acaggccgat gtctgggacc cattcgcggg
     2041 tgtca
//
LOCUS       DASRKH010000265         2021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_688, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000265 DASRKH010000000
VERSION     DASRKH010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="VFC06_04295"
     CDS             complement(<1..875)
                     /locus_tag="VFC06_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HZL81146.1"
                     /translation="MNHNDIPDVTPDEELVPFEVDDAEADQRAAALRAGLAEFELSEE
                     DRALLEEGGDGGATVRARPGLPVLAVVGRPNVGKSTLVNRILGRREAVVQDTPGVTRD
                     RVKYPAEWAGREFTVVDTGGWEVDVAGIDARVAEQAEVAVELADAVLLVVDGTVGATE
                     TDTQVIRMLRREGKPVVLAANKVDGATGESDAATLWSLGLGQPYAISALHGRGIGDLL
                     DAALAVLPAVSAVAGPQPEGGPRRVALVGRPNVGKSSLLNAVAGAERVVVHDVPGTTR
                     DPVDEIITLGGRQWM"
     gene            complement(930..1622)
                     /locus_tag="VFC06_04300"
     CDS             complement(930..1622)
                     /locus_tag="VFC06_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZL81147.1"
                     /translation="MTSLGQSVRAADIKRWGPVWSRRVGWFLDHIWWNTTVLGAENVP
                     STGPVIVASNHIGVVDGPVLHGAIPRGSHIIVKQEFFDSKLGFLMDWAGQIPVDRSSG
                     RAALVVALEMLKEGRVVGIFPEGTRGTGDVQSVRAGVAWLALNSGAPLVPAACLGTRP
                     AGATVGHVPKPRAHLHVVFGEPLTLPADLPRGKAGTQEAMRLVSEHMTAHVADAVART
                     GIALPGDAGTRD"
     gene            complement(1619..>2021)
                     /locus_tag="VFC06_04305"
     CDS             complement(1619..>2021)
                     /locus_tag="VFC06_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HZL81148.1"
                     /translation="VSIVAVNLAVRAELRDRQRAIIDVETTAGRSGGRGIVAEGRDIT
                     TVVAPDADVRILLIAEPGDRLARRARERHGNADTRSIEATRDEVIRRDAQDSTVSEFM
                     RADDGVVTLDNSGMEPDETFAAAIAIVESQR"
BASE COUNT          277 a          777 c          679 g          288 t
ORIGIN      
        1 aacatccact ggcgcccgcc gagcgtgatg atctcgtcca cgggatcgcg cgtcgtaccg
       61 ggaacgtcgt gcaccaccac acgctcggcg ccggccaccg cattcagcag cgatgacttc
      121 cccacgttgg ggcggcccac cagggccacg cgccgcggcc cgccctccgg ctgcggcccg
      181 gcgacggcgg acaccgccgg cagcaccgcg agtgcggcgt cgagcaggtc accgatgccg
      241 cggccgtgca gcgccgagat cgcgtacggc tgtccgagac ccagcgacca gagcgtcgcg
      301 gcgtccgact ccccggtggc cccgtccacc ttgttggcgg cgagcaccac cggcttgccc
      361 tcacggcgca gcatccggat cacctgcgtg tcggtttccg tcgctcccac ggtgccgtcc
      421 accaccagca gcacggcgtc agccagttcc acggccacct ccgcctgctc ggcgacgcga
      481 gcgtcgatgc ccgcgacgtc cacctcccag ccacccgtgt ccaccaccgt gaattcgcgc
      541 ccggcccatt ccgccgggta cttcacgcgg tcgcgggtca ccccgggggt gtcctggacc
      601 accgcctcac ggcgcccgag aatgcggttg accagcgtcg acttgcccac gttcggccgc
      661 ccgaccaccg ccaggaccgg caggccgggc cgggcccgca ctgtcgcgcc gccgtcgccg
      721 ccctcctcca ggagtgcgcg gtcctcctcg gagagctcga attcggccag accggctcgc
      781 agcgccgcgg cccgctggtc ggcctcggcg tcgtccacct cgaaggggac caattcctcg
      841 tcaggggtga cgtcggggat gtcgttgtgg ttcactgcgc aattgtccca cgcggggagc
      901 gccgcccaat cggggcggga agagcgtggt cagtcccgcg tgccggcgtc gccgggcagt
      961 gcgatgccgg tgcgggccac cgcgtccgcc acgtgagccg tcatgtgctc ggacacgagc
     1021 cgcatggcct cctgcgtgcc ggcctttccg cgcggcaggt ccgccggcag ggtcagcggc
     1081 tcgccgaaca ccacgtggag gtgcgcccga ggcttcggga cgtgccccac cgtggccccg
     1141 gcgggccggg tgcccaggca cgcagcgggc accaagggcg ccccggagtt gagcgccagc
     1201 caggcgactc cggccctgac ggactgcaca tcgcccgtgc cgcgggtgcc ctctgggaag
     1261 atccccacca cgcggccctc cttcagcatc tcgagcgcca ccacgagggc ggcccggccg
     1321 gaggaccggt ccacggggat ctgccctgcc cagtccatga ggaacccgag tttcgagtcg
     1381 aagaactcct gcttcacgat gatgtgcgaa ccgcggggaa tggccccgtg gagcaccggc
     1441 ccgtcaacca cgccgatgtg gttggaggcc acgatcaccg gtccggtgct cgggacgttc
     1501 tcggctccga gcacggtggt gttccaccag atgtggtcca ggaaccagcc cacccgccgg
     1561 gaccacaccg ggccccagcg tttgatgtcc gccgctcgaa ccgactggcc gaggctggtc
     1621 atcgctgaga ctccacgatc gcgatcgccg ccgcgaaggt ctcgtccggc tccatgccgc
     1681 tgttgtcgag ggtgaccacg ccgtcgtccg cgcgcatgaa ttccgagacg gtcgagtcct
     1741 gggcgtcccg gcggatcacc tcgtcgcgag ttgcctcgat cgacctcgtg tccgcgttgc
     1801 cgtggcgctc ccgtgcacgg cgcgccagcc ggtccccggg ctcagcgatg aggaggatgc
     1861 ggacgtccgc gtctggtgcg accacggtgg tgatgtcccg gccctcggcc acgatccccc
     1921 ggcccccgga gcgacccgct gtggtctcga catcgatgat ggcacgctgg cggtcccgca
     1981 gttccgcgcg caccgcgagg ttgaccgcga caatggagac c
//
LOCUS       DASRKH010000266         2376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_51604, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000266 DASRKH010000000
VERSION     DASRKH010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51604"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="VFC06_04310"
     CDS             complement(<1..588)
                     /locus_tag="VFC06_04310"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family cytochrome oxidase biogenesis
                     protein"
                     /protein_id="HZL81149.1"
                     /translation="MTSPAAKHRPAAYRPRRIALIVIVVLAAMALGLIAGKWQWGRYE
                     TKSQALAGQAAAEGKPAANLEELIARDAVGPGAAEWRTATVTGAIDPASLTELRGRRV
                     ANTASLQYLVWIHTDTGRTVMVNLGWQPRSEATPPALPGEQVTVTGIVRAFEADNGKP
                     GTRITPRHMNGIAGEVVQGYLMARSTCSESAPARGH"
     gene            653..1357
                     /gene="fabG"
                     /locus_tag="VFC06_04315"
     CDS             653..1357
                     /gene="fabG"
                     /locus_tag="VFC06_04315"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HZL81150.1"
                     /translation="MTRHVVVTGANRGIGRAIAEAFVANGDTVSSIYRGGDLPDGVTG
                     AIADITDTDAVNAAFDELEAKHGRVRVLVANAGITRDGLLMRMSDEDFAAVIDTNLTG
                     TFRTVRRAVNSMMREKYGRIVLVGSVVGLLGNPGQVNYSASKSALVGMARSITREVGG
                     RGITANVVAPGFIATDMTAELGDDAIKQYESSIPAKRFGTTHDVAAATVFLASDAAGY
                     ISGAVLPVDGGLGMGH"
     gene            1394..2161
                     /gene="fabI"
                     /locus_tag="VFC06_04320"
     CDS             1394..2161
                     /gene="fabI"
                     /locus_tag="VFC06_04320"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020013108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HZL81151.1"
                     /translation="MGILEGKNILVTGVLTEGSIAFDVARLSQEQGAKVVLTGVGRTL
                     KVTEAMGRRLPVEAKVVELDVTNPDHLAALPDAVREHVPHLDGVVHSIGFAPQTVMGG
                     NFMTGQWEDVSTAIHISAYSLKALAEATVPLMPNGGSIVGLTFDGRFAWPVYDWMGVA
                     KSAFEAISRYLARDLGPQGIRCNLVSAGPLKTTAAKHVPGFDQMESKWSERSPLGWDS
                     EDTEPAAKAVTALLSDWFPATTGEMVHVDGGVHAMGQ"
     gene            2164..>2376
                     /locus_tag="VFC06_04325"
     CDS             2164..>2376
                     /locus_tag="VFC06_04325"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HZL81152.1"
                     /translation="MPRLVLARHAKAEAPQRGLGDHSRALTVQGRDDAARLAERLQAL
                     GLHPDVALVSSANRTVQTFKRMATVWD"
BASE COUNT          394 a          772 c          800 g          410 t
ORIGIN      
        1 atgccctcga gccggtgccg actcgctgca ggtcgacctc gccatcaggt atccctgcac
       61 tacctcgccg gcgatgccgt tcatgtggcg cggggtgatg cgtgttccgg gcttgccgtt
      121 gtccgcctcg aaagcgcgga caatgccagt gacagtgact tgctcgccgg gcaatgccgg
      181 gggagtggcc tcggatcgag gttgccatcc caagttcacc atgacggtgc ggcccgtatc
      241 cgtgtggatc cacacgaggt actgcagcga tgccgtgttg gctacacggc gcccacgcaa
      301 ttccgtgagc gacgcggggt cgatcgcgcc ggtcaccgtc gcggtccgcc attccgccgc
      361 cccggggccc acagcatccc gagctatcag ctcctcgagg ttcgccgccg gtttgccctc
      421 ggccgcagcc tgacccgcga gcgcctgcga cttggtctcg tagcggcccc actgccattt
      481 gccagcgata aggcccaaag ccatcgcggc caacacgacg atcacgatga gcgcgatccg
      541 tcgcggccgg tatgcggccg ggcggtgttt cgcggcggga ctggtcacgg tgtcgatcgt
      601 acgtgcgagg cgagtgcgcg aggtgacggg cgcatcggct agtttggggc ccatgacgcg
      661 acacgtggtg gtgacaggcg cgaaccgcgg catcggccgt gcaatcgcgg aagccttcgt
      721 ggcaaacggg gacaccgttt ccagcatcta caggggcggc gatctgccgg acggggtgac
      781 cggcgcgatc gccgacatca ccgacaccga cgccgtcaac gccgcctttg atgaactaga
      841 ggccaagcac gggcgtgtgc gcgtgctcgt ggccaacgcg ggcatcactc gcgacggact
      901 gctgatgcgg atgtccgacg aagattttgc cgcggtcatc gacaccaacc tcaccggtac
      961 tttccgtacg gtgcgccggg cggtcaactc gatgatgcgg gagaagtacg gccggatcgt
     1021 cttggtcggc tcggtggtgg gattgctcgg caatcccggt caggtcaact actcggcatc
     1081 caagtctgcg ctcgtgggca tggcgaggtc catcacgcgc gaggtgggtg gacgcggcat
     1141 caccgccaac gtggtcgcgc caggattcat cgcgacggac atgacggccg aacttggcga
     1201 cgatgcgatc aagcaatatg agtcatcgat tccggccaag cggttcggta ccacccacga
     1261 cgtcgctgcg gctaccgtat tcctggcctc cgatgcggca ggttacatat ctggcgcggt
     1321 gctgcccgtt gacggcggac tgggcatggg tcactagttt ccggactacg tagtctttcc
     1381 gacaggagga accatgggca ttctcgaagg caagaacatc ctcgttaccg gggtacttac
     1441 cgaaggttcg atcgcgttcg atgtggcgcg actgagccag gaacagggcg ccaaggtggt
     1501 gctgaccgga gtgggtcgca ccctcaaggt caccgaggcg atgggccgca ggctgccggt
     1561 cgaggccaag gtcgtcgagc tcgacgtcac caaccccgac cacctggctg cgcttccgga
     1621 cgccgtgcgc gagcacgtgc cgcatctcga cggcgtggtc cactcgattg gcttcgcccc
     1681 ccagacggtg atgggtggca acttcatgac cggacagtgg gaagacgtct ccacggccat
     1741 ccacatctcg gcctactcgc tcaaggctct tgcggaggcg accgtgccgc tcatgccgaa
     1801 tggcgggtcc atcgtcgggc tcacgtttga cggacgcttc gcgtggcccg tgtacgactg
     1861 gatgggtgtc gctaagtcgg ccttcgaggc gatcagccgc tacctggcgc gcgaccttgg
     1921 cccgcagggc atccgctgca acctggtctc cgctggaccg ctcaagacca cggccgcaaa
     1981 gcacgtcccc ggcttcgacc agatggagag caagtggagc gagcgctcgc cgctcggctg
     2041 ggactcggag gacaccgagc ctgcagccaa ggccgtcacc gccttgctca gcgactggtt
     2101 ccctgcgacg accggcgaaa tggtccacgt cgatggcggc gtccacgcca tgggccagta
     2161 gcagtgccca gactggttct ggccaggcac gctaaggccg aggctcctca gcggggactc
     2221 ggcgatcatt cgcgcgcgct cactgtgcag ggccgggacg acgccgcacg gctcgcggag
     2281 cggttgcagg ccctggggct gcacccggac gtggcgcttg tctcctcggc gaatcgcacc
     2341 gtccagacgt ttaagcgcat ggccacggtg tgggac
//
LOCUS       DASRKH010000267         2531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_16053, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000267 DASRKH010000000
VERSION     DASRKH010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16053"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="VFC06_04330"
     CDS             complement(<1..801)
                     /locus_tag="VFC06_04330"
                     /inference="COORDINATES: protein motif:HMM:NF024573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZL81153.1"
                     /translation="MRILQVRIENYRVHRSTIVDFDGLTAFIGRNGAGKSSVLYAIDF
                     FYDVSAVLSGDDVYAGADEEVAITVTYSNLSQAELDEFGLYIRGDVLTVIKRARAGQP
                     GRYYGIVPQLPEFVEVRAISGAMPQRTAYKEVCESGRFPSLPSVGNQSELVAAMDEFE
                     RDPANAAHLQPIERLEQFFGDRRAGAGRLDNFTSFVLVPAVHEAASESEKRGAIQTLV
                     DRLVSSALANRDDIVQFRTEFEERFAATYAPENLTEIERVSDAINELLG"
     gene            complement(815..1135)
                     /locus_tag="VFC06_04335"
     CDS             complement(815..1135)
                     /locus_tag="VFC06_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81154.1"
                     /translation="MIEHEDFVECVRERKRARVVFASQKEGGTLRERICAPMDFGPDR
                     RAADTRDRYHFFDLDAGHLLKKFPKDIVRFEPLGQTFEPADFVTWDVRQSPWWLKRDW
                     GVYS"
     gene            complement(1209..1967)
                     /locus_tag="VFC06_04340"
     CDS             complement(1209..1967)
                     /locus_tag="VFC06_04340"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZL81155.1"
                     /translation="MTADEARKTATRQALIDAAAQVFLERGFARATTREIARAAGVAE
                     GTIYRHFDDKYALFHEVFLSRAGDTVEELARFPHRAGQSSVRDNLGQLLELIGRMVEH
                     TTSLMASMWADPDVARRFEAYVRERAPKGLEAGPVAIVAAYLRAEQELGRIRDDVDAA
                     EAAAVVASVPFANGMERALRERFPSPGDFPAPGRAALDILVQGLGPTMGVSASHGQGN
                     TEGSPLPANQAFEQTRRSSAQDRSVGSRSSTPGR"
     gene            complement(1996..>2531)
                     /locus_tag="VFC06_04345"
     CDS             complement(1996..>2531)
                     /locus_tag="VFC06_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81156.1"
                     /translation="SVTSDYSSGLIRLLVQAEPRRLRLFGGKVVALTAFTCLATLVAT
                     VVVLLVSPAIASLTGVSMHAWSVDLPHTVVEGYLQLTLSVLLWGFVGLFVGVATRSSG
                     ISIGIGIGYLLVFEGVVGMLLDNAAKWLPGATFTAIASGGTTDMAFATALLIGAAYAA
                     VAIAASAITFRRRDITA"
BASE COUNT          492 a          810 c          778 g          451 t
ORIGIN      
        1 acccagaagc tcgttgatgg cgtcggatac gcgctcgatc tcggtcaagt tctcgggcgc
       61 ataggttgct gcaaaccgtt cctcgaactc cgttctgaac tgcacgatgt catctctgtt
      121 cgcaagagcg gatgagacga gacggtcaac cagagtctgg attgcgcctc gcttctcgga
      181 ttcactggcc gcctcgtgaa ccgcgggcac caatacgaac gacgtgaagt tgtcgaggcg
      241 accggcccct gctctgcggt cgccgaagaa ctgctctagg cgctcgatgg gctgaaggtg
      301 cgccgcgttg gcagggtcgc gttcgaactc atccatcgcg gcaaccagct ctgattgatt
      361 cccaactgaa gggaggctcg ggaagcgccc tgattcgcag acctccttgt aggcggtgcg
      421 ttgcggcatg gcacccgata tggcgcggac ctcaacgaac tcgggaagct gcgggacgat
      481 gccatagtac ctgcccggtt ggcctgctcg agcgcgtttg atcacagtca gaacgtcgcc
      541 gcggatatac aggccgaact cgtcgagttc cgcctgcgac agattcgagt aggtgaccgt
      601 gatcgccact tcctcgtccg cgccggcata tacgtcatca ccgctgagta ctgcgctgac
      661 gtcgtagaag aagtcgatcg catacaggac agacgatttc ccggctccat tacggccgat
      721 gaaggcagtc aggccgtcaa agtccacgat ggtggagcga tgcacgcgat agttttcgat
      781 gcggacctgt aagatcctca tgacgtagcc tgtctcacga gtaaactccc cagtcccgct
      841 tgagccacca tggactctgg cgcacgtccc acgtcacgaa atccgcaggc tcgaaggtct
      901 ggcctaaggg ctcaaaccgg actatgtctt tggggaactt cttcagcaga tggcctgcat
      961 ccaggtcgaa gaagtggtac ctgtctcgcg tgtccgcagc ccggcggtca ggcccgaagt
     1021 ccatgggagc acagatgcgc tcgcggaggg tgccaccttc cttctgggac gcgaacacga
     1081 cccttgctct cttgcgctcg cggacgcact ccacgaagtc ctcatgctca atcatgttgc
     1141 ctcccccttg actcccccac gatcaacgcc caactctgac aagcgaccgt tgtgtgacgt
     1201 gcatggccct aacgtccggg cgttgagctg cgagacccaa cgctccggtc ttgcgccgag
     1261 cttcggcgag tctgctcgaa cgcctggtta gcgggaagag gggagccttc ggtattgcct
     1321 tggccatgac tcgccgacac gcccatggtc ggccccagtc cctgaacaag gatgtcgaga
     1381 gctgcgcgtc ccggcgccgg gaagtcccca ggtgaaggga agcgctcccg cagagcacgt
     1441 tccattccgt ttgcgaacgg caccgacgcg accaccgcag cggcctcggc agcgtcaacg
     1501 tcgtccctga tcctgcccag ttcctgctcc gctcgcaaat aggcggcaac gattgcgact
     1561 ggtcccgcct cgaggccctt gggcgcacgc tcccggacat aggcctcgaa gcgcctggcc
     1621 acgtcggggt cggcccacat cgacgccatc agcgaggtgg tgtgctccac catgcggccg
     1681 atcaactcaa ggagttgccc gagattgtct cgtacgctgc tctggccggc acggtgcggg
     1741 aatcgcgcca gctcctccac ggtgtcgccg gcgcgagaca ggaagacctc gtggaagagc
     1801 gcgtacttgt cgtcgaagtg ccggtagatg gtgccctcgg caacaccggc ggctcgcgcg
     1861 atttccctgg tggtcgcgcg cgcgaaaccg cgctcgagaa acacttgagc cgccgcgtcg
     1921 atcaaggcct gtcgcgttgc tgtcttgcgc gcttcatcag cggtcatcgg tcctcctcaa
     1981 gagcatgcgc ccgactcatg ccgtgatgtc acgacgacgg aacgtgatcg cagacgccgc
     2041 gatcgccact gcagcatacg ctgcgccgat gagcaaggcc gtagcgaacg ccatatcggt
     2101 ggtccctccg ctggcgatgg ccgtgaaggt cgctccgggc agccacttcg cggcgttgtc
     2161 cagaagcatg ccgaccacac cctcgaacac gagcaggtag ccgatgccga tgccgatcga
     2221 gatgcctgag ctgcgcgtgg cgacgccgac gaacagccca acgaagcccc aaaggagaac
     2281 agaaagggtc agctgaaggt agccttcgac cacggtgtga ggaagatcga cgctccaggc
     2341 gtgcattgag actcccgtca gactcgcgat ggccggcgag acgagcaaga cgacgacggt
     2401 cgcgaccagc gtagccaagc aggtgaatgc ggttagagcc acgaccttgc cgccaaacag
     2461 gcgcaagcgg cgaggctcgg cttgcacgag caggcggatc aggccactcg agtagtcgga
     2521 cgttacggag a
//
LOCUS       DASRKH010000268         2820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_18099, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000268 DASRKH010000000
VERSION     DASRKH010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18099"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1391
                     /gene="rho"
                     /locus_tag="VFC06_04350"
     CDS             <1..1391
                     /gene="rho"
                     /locus_tag="VFC06_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HZL81157.1"
                     /translation="RSQRGNRNQRGGTDRNQGGSDRNQGGSDSSHNNSNQDDDDRSGR
                     RRRGRGRARGRGPETPQQGGTTGPVEVYDEDDVRDDEELTPVGGILDVLDNYGFIRTT
                     GYLPGKTDVYVSMNQVRKYGMRKGDAVTGAARLPRAGERGQRQKFNALARVDAVNGMT
                     AEEANARPAFGDLTPLYPQARLHLETDPRNLTNRVIDLVSPIGKGQRGLIVSPPKAGK
                     TLVLQSIANAIAVNNPEVHLMVVLVDERPEEVTDMQRTVKGEVIASTFDRPAEDHTTI
                     AELAIERAKRLVEMGQDVVVLLDSITRLGRAYNLAAPASGRILSGGVDSSALYPPKRF
                     FGAARNIEHGGSLTILATALVETGSKMDEVIFEEFKGTGNMELKLSRQLADRRIFPAV
                     DVNSSGTRREEILISPEELKVSWKLRRVLTGLEQQQAIELLLGRLKENKTNAEFLMTV
                     AKTTPPGPEDKE"
     gene            1498..1707
                     /gene="rpmE"
                     /locus_tag="VFC06_04355"
     CDS             1498..1707
                     /gene="rpmE"
                     /locus_tag="VFC06_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015772057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HZL81158.1"
                     /translation="MKKDIHPEYVATTVTCTCGNKFETRSTITSGIVSVEVCSACHPF
                     YTGKQKIMDTGGRVARFQKRYGTKD"
     gene            1729..>2820
                     /gene="prfA"
                     /locus_tag="VFC06_04360"
     CDS             1729..>2820
                     /gene="prfA"
                     /locus_tag="VFC06_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HZL81159.1"
                     /translation="MAEAFAAVQPLLDEYEDIERQLSDPAVHADGGRARTLGRRFAEL
                     GRVVAAHHVWEAAQDDLAAGRDMAEADPDFADEIPALAAAVENATETLRRILIPRDPD
                     DARDVILEIKSGEGGEESALFAGDLLKMYLKYAETKGWKAEVLEATETDMGGFKDISV
                     ALKSRGNPDPQDGVWAQLKYEGGVHRVQRVPATESQGRIHTSAAGVLVYPEADEVEEL
                     EIDMNDVRVDVFRSSGPGGQSVNTTDSAVRLTHEPTGIVVSCQNEKSQLQNKESALRI
                     LRARLLAAQKEAADAEAHDIRKSQVRTVDRSERIRTYNFPENRIADHRTGYKAYNLDQ
                     VLGGDLGPVIQSAIDADWITGPRSPPSTWS"
BASE COUNT          549 a          965 c          907 g          399 t
ORIGIN      
        1 accgcagcca gcgcggcaac cgcaaccagc gcggcggcac cgaccgtaac cagggcggca
       61 gcgaccgcaa ccagggcggc agcgacagct cgcacaacaa ctccaaccag gacgacgacg
      121 atcgttcggg tcgcaggcgt cggggccgcg gccgcgcccg tggccgcggc cccgagaccc
      181 cgcagcaggg cggtaccacc ggccccgtcg aggtgtacga cgaggacgat gtcagggacg
      241 acgaggagct gaccccggtc ggcggcatcc tggatgtgct cgacaactac gggttcatcc
      301 gcaccaccgg ctacctgccc ggcaagacgg acgtctacgt ctccatgaac caggtgcgca
      361 agtacgggat gcgcaagggc gacgccgtga ccggcgcggc ccgactgcct cgcgcggggg
      421 agcgtggcca gcgtcagaag ttcaacgctc tggcccgcgt cgacgcagtc aacggcatga
      481 cggccgagga ggccaatgct cgcccggcat tcggcgacct gaccccgctg tatccacagg
      541 cgcgcctcca cctcgagacg gaccctcgca acctcaccaa ccgcgtgatc gacctggtct
      601 cgccgattgg taagggccag cgtgggctca tcgtctcgcc acccaaggca ggcaagaccc
      661 tcgtactgca gtcgatcgcc aacgcgatcg cggtcaacaa ccccgaggtt cacctcatgg
      721 tggtgctggt ggacgagcgg cctgaagagg ttacggacat gcagcgcacg gtcaagggag
      781 aggtcatcgc ttcgaccttc gaccgccctg ccgaggacca caccacgatc gccgaattgg
      841 ccatcgagcg tgccaagcgt ctggtcgaaa tgggccaaga cgtggtggtg cttctcgact
      901 ccatcacccg cctgggccgc gcctacaacc tggccgcccc cgcatcgggc cgcatcctgt
      961 ccggtggtgt cgactcgtcg gcgctgtatc cgcccaagcg cttcttcggc gccgcgcgca
     1021 acatcgaaca cggcggctcg ctcaccatcc ttgcgaccgc gctcgtcgag accggctcca
     1081 agatggacga ggtcatcttc gaagagttca agggcacggg caacatggag ctcaagctct
     1141 cgcgccagct ggccgaccgt cgcatcttcc ccgcagtcga cgtcaactcg tctggaacgc
     1201 gccgcgagga gatactcatc agccccgaag agctcaaggt gagctggaag ttgcgccgcg
     1261 tgctcacggg cctcgagcag cagcaggcca tcgagttgct cctcggaagg ctcaaggaga
     1321 acaagaccaa tgccgagttc ctgatgaccg tggcgaagac cacgcctcca gggccggaag
     1381 acaaggagta gtcccgggca caaagggacg ctcacgagcg ttacactgtt tcgctggctg
     1441 gccggttcac cgtcgcaagg tatgtgtatg gacccggcag caacctcaag ggagatcatg
     1501 aagaaggaca tccaccccga gtacgtggcc accactgtca cgtgcacctg cggtaataag
     1561 tttgagaccc gcagcaccat cacctctggc attgtcagcg ttgaggtctg ctcggcttgc
     1621 cacccgttct acacgggcaa gcagaagatc atggacaccg gaggccgcgt agcccgcttc
     1681 cagaagcgct acgggaccaa ggactaagcg accgggcgtc gcgacaacgt ggcagaagca
     1741 ttcgcagccg tccagccgct gctggacgaa tacgaggaca ttgagcgcca gctctccgac
     1801 cccgcggttc acgccgacgg ggggcgggcg cgcaccctcg ggaggcgttt tgccgagcta
     1861 ggacgcgttg tcgcggcgca ccacgtctgg gaggcagccc aggacgatct ggccgccggc
     1921 agggacatgg ccgaggccga tcccgacttt gccgacgaga ttccggccct tgcggccgca
     1981 gtcgagaacg ccacggagac gctgcgtcgc attcttatcc cgcgcgaccc cgacgacgcc
     2041 cgcgacgtga ttctcgagat caagtcaggc gagggcggcg aggaatctgc gctgttcgcc
     2101 ggcgacctgc tgaagatgta cctcaaatac gccgagacca aaggctggaa ggccgaggtg
     2161 cttgaggcga ccgagacgga catgggcggg ttcaaggaca tctccgtggc cctcaagtcc
     2221 aggggtaacc ccgatccgca agacggcgtg tgggcccaac tcaagtacga gggcggcgtg
     2281 caccgcgttc agcgcgtgcc ggccacggag tcgcagggcc gcatccacac ctccgccgcg
     2341 ggcgtgctcg tgtaccccga ggccgatgag gtcgaggagc ttgagatcga catgaacgac
     2401 gtgcgcgtcg acgtgttccg ctcgtccggc cccggcgggc agtccgtcaa caccacggac
     2461 tcggccgtgc gcctcaccca cgagcccacc ggcatcgtgg tgagctgcca gaacgagaag
     2521 agccagctcc agaacaagga gtccgcgctc cggattctgc gcgcccgcct cttggccgcg
     2581 cagaaggaag ccgccgacgc tgaggcccac gacattcgca agtcgcaggt gcgcaccgtg
     2641 gaccgttcgg agcgcatccg cacctacaac tttccggaga accgcatcgc ggatcaccgc
     2701 accggttaca aggcgtacaa cctcgaccag gtgctcggcg gcgacctggg ccccgtgatc
     2761 cagtcggcga tcgacgccga ctggatcacg gggcccaggt cgccgccgag cacctggtcg
//
LOCUS       DASRKH010000269         2441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_25249, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000269 DASRKH010000000
VERSION     DASRKH010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25249"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VFC06_04365"
     CDS             <1..306
                     /locus_tag="VFC06_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81160.1"
                     /translation="LAKDARLDGGYVVERVHEIAKRLPGAFEGAFASVGSEADTLRNR
                     TMPRLKEHVAGVAGRATRPVSATASSSAGDSARGDKTWVPEHVRNGRRVAGHWRRNR"
     gene            532..1197
                     /locus_tag="VFC06_04370"
     CDS             532..1197
                     /locus_tag="VFC06_04370"
                     /inference="COORDINATES: protein motif:HMM:NF013938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81161.1"
                     /translation="MLTVLAADEDSFVRARVATNPATPLDVLTVLATDEEWEVRNGVA
                     KNPSAPPVVLVELAQDENWGVRSSVAANSATPTDVLVTLSAYEKVPVLRGVAANPSTP
                     AKVLVALATDESTRGPVAANPSTPPEVLMELAHDDYEQARENVANNPSTPPEVLMELA
                     HDENRDVRYGARRAIATHICEAFDIDETNTEALDLLRDEQWWTMDTDSPAVVLAKALH
                     PNA"
     gene            1397..1894
                     /locus_tag="VFC06_04375"
     CDS             1397..1894
                     /locus_tag="VFC06_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81162.1"
                     /translation="MEELQTYQWKKEPPDPFVRAHVALAMDLALAVCPSTKSLVWAPV
                     YDPDFKSALYTPTAECTHFDGIGAIDDFVLAHGLGTADADTLAETLQWTPRDYEMKAM
                     ADELGLEPHGDEWHQSRNVAVSRATGIDRDNREAMAMFADVDPRTISATDPDIELAKA
                     LHPDA"
     gene            2121..>2441
                     /locus_tag="VFC06_04380"
     CDS             2121..>2441
                     /locus_tag="VFC06_04380"
                     /inference="COORDINATES: protein motif:HMM:NF013938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81163.1"
                     /translation="MALAADTPPATLELLSQDWDAGVRKAVARHADAPLSALESLAFD
                     GDVSIRRAAAAHESTPPHVLEELFDDHDGAVSWRAKINPSASEESLERLEGDHGDLSA
                     VKALT"
BASE COUNT          457 a          802 c          811 g          371 t
ORIGIN      
        1 ctggccaagg atgcacgcct ggacggcggg tacgtggtcg agcgggtgca cgagatcgcg
       61 aagcggctgc ccggtgcctt cgagggtgcc ttcgcctcgg tgggttcgga ggcagacacc
      121 ctccgtaacc gcaccatgcc ccgcctgaaa gagcatgtgg cgggtgtggc cgggagggcg
      181 acgcgtccag tgtccgcgac ggcatcgtcg agcgctggcg actccgcgcg tggcgacaag
      241 acatgggtgc ccgagcatgt tcgcaatggg cgccgcgtcg cagggcactg gcgacggaac
      301 cgatagtcgc gcacgagcgg gcgtggatac cgtgaagcca tgagcatttg tagcgcgccg
      361 gccaagggtc accggaagcc ggggtctgcg gcgaagtgcc ccgtgcacgg ccccggtccc
      421 ggcgctcgcg ttcaaaaact cccgtcgacg gccaggaccc aggcggtggt tttacctgaa
      481 cctccgggga aacgcggctt gctcgccgga agccctgcaa cgcctcacga tgtgctgacg
      541 gtgctggctg cggatgaaga cagtttcgtg cgcgcgcgtg ttgccacaaa cccagcgaca
      601 ccactcgatg tgctgacggt gctggctacg gacgaggaat gggaagtgcg caatggcgtc
      661 gccaagaacc cttccgcgcc tccggtcgtg ctggtggaac tcgcgcagga cgagaactgg
      721 ggcgtgcgct cgagtgtggc cgcaaactca gcaaccccaa ccgacgtgct ggtgaccttg
      781 tccgcgtacg agaaagtgcc agtgctcagg ggcgttgccg caaacccctc cacaccggcc
      841 aaggtgttgg tggcgctggc tacggatgag agtacgcgcg gccccgttgc cgcaaacccc
      901 tccacaccgc ccgaggtgtt gatggaactc gcgcacgacg attacgagca ggcgcgcgag
      961 aatgtggcca acaacccctc cacaccgccc gaggtgttga tggaactcgc gcacgacgag
     1021 aaccgggatg tgcgatacgg cgcaaggcgc gcgatcgcca cccacatctg tgaagcgttc
     1081 gacatcgacg aaacaaacac cgaggctctc gacctactgc gtgacgagca gtggtggacc
     1141 atggataccg acagtccagc cgtggtgctg gccaaagcgc ttcacccgaa cgcatgagcg
     1201 tggatacctt caagccatga gcgaatgcac cgcaccaatc gaaggtcaca agggcgactc
     1261 tgctgccgca gccaggtgtc ccgtccatgg gaatcgcggc catgcagcgc cagtggccgc
     1321 gcctgccgct ctccctgtgc tctctccgac cacaccgcag gagcgaatcc gcgtaacact
     1381 gcgggacgca cccgacatgg aggaactaca gacgtaccag tggaagaaag agcctccgga
     1441 tccgttcgta cgagcgcacg tggcgcttgc aatggacctg gccctggctg tgtgcccttc
     1501 cacgaagtcc ctcgtctggg cgcccgtgta tgacccggat ttcaagtcag cgctctacac
     1561 gccgaccgcc gagtgcacac acttcgacgg gataggcgct atcgatgact tcgtgctggc
     1621 tcatggcctc gggacggcgg acgctgacac gctcgccgag acactgcaat ggacgccgcg
     1681 cgactacgag atgaaggcca tggccgacga actcggcctc gagccgcacg gcgatgagtg
     1741 gcaccagtct cgcaacgtgg ctgtctcgcg tgcgactggg atcgaccggg acaaccgaga
     1801 agcgatggcc atgttcgccg acgtggaccc caggacgatc tccgccacgg atccggacat
     1861 cgaactcgcg aaggcgctgc acccggacgc ctaggcccga aacgtgttgc ggcagcacgg
     1921 ctcgcaaagt gtctacgcgg cgctggcagg agcgctcggg gcgtggatac ctttgagcca
     1981 tgagccccaa atgcagtgcg ccagtgttgg gccaccgcac ggcgcgctcg ttccttagtt
     2041 gccccgtaca tggggcgaca ccggcccgct cagcaactga cggcgttccg gcgacggcga
     2101 cacgggatga gcgcctcctc atggccctcg cggctgatac gccacctgcg accctagaac
     2161 tgctgtcgca ggactgggac gcaggcgtgc gcaaggcggt cgcccgtcac gccgacgcgc
     2221 ccctgagcgc cctggaatcc ctcgcgttcg acggggacgt gtcgatccgg cgcgcggcgg
     2281 ccgcccacga gtcgacgcca ccgcacgtgc ttgaggagtt gttcgacgac cacgacggcg
     2341 cggtttcgtg gcgggcgaag atcaaccctt ccgcctcgga ggagtccctc gaaaggctcg
     2401 aaggcgatca cggtgatctc agcgcggtga aggccctgac g
//
LOCUS       DASRKH010000270         2648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_38453, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000270 DASRKH010000000
VERSION     DASRKH010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38453"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /gene="infC"
                     /locus_tag="VFC06_04385"
     CDS             complement(<1..312)
                     /gene="infC"
                     /locus_tag="VFC06_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HZL81164.1"
                     /translation="MNEPRINERIRVPEVRLVGPQGEQVGIVRIEDAMRLAQESDLDL
                     VEVAANSRPPVVKLMDYGKFKYEAAVKAREARRNQANTEIKEMRFRLKIDEHDYETKK
                     GH"
     gene            520..900
                     /locus_tag="VFC06_04390"
     CDS             520..900
                     /locus_tag="VFC06_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019483420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HZL81165.1"
                     /translation="MNSPTPSPDSQNPSPSLGDPLVAARDIADVSAIELITTVSVHLM
                     SAAALRCGLADEGEEFQDLSEARIMINALAALVTGASPDLGDTHARALRDGLRSVQLA
                     FREASVVPDAPGEGPGEKYTGAVN"
     gene            901..1530
                     /locus_tag="VFC06_04395"
     CDS             901..1530
                     /locus_tag="VFC06_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81166.1"
                     /translation="MTTTPSHDNGKISRTAANEETTVLTPMSDGATPLERMPVKRQRL
                     WIIVTSLLGVLLITAGIIIYRLVDVSNLWEAQVEDVKAQNYDLGERLAEEQAQGIELQ
                     GQVDLVNEQLRTAQQRVLELADDVAQRDDNAEFYARQITDLTDVLTTASAVANSLNKC
                     VDYHQQLVDILKQPDNYLPDEVATFEAGVTKVCDAATAANVDLQTVLAQ"
     gene            1527..2261
                     /locus_tag="VFC06_04400"
     CDS             1527..2261
                     /locus_tag="VFC06_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZL81167.1"
                     /translation="MRSPRALLAACAVAGLLAGCAALPDAPSPLPSDYVPAAPVVDVA
                     QQGNLSPDGFSQAQRMTVRVRNVGCDSLKTGTGFAYDAHTLITNRHVVEDTSELQVTT
                     YDGRTIAVTAASVAAVADLAIITTEESIGAGAVLAQEDPVEGDAITVVGYPNGGQLTT
                     VSGVVLGATGDPLGAALGTVLATTAPVEPGSSGSPVLNERGEVVGVIYAKNEAEQSFM
                     VPVSLLRTLLDEDQLLVPQQQSCNPS"
BASE COUNT          481 a          908 c          822 g          437 t
ORIGIN      
        1 gtgacccttt ttggtctcgt agtcgtgctc gtcgatcttg agacggaaac gcatctcttt
       61 gatctcggtg ttggcttggt tgcgacgcgc ctcgcgcgcc ttgaccgccg cttcgtactt
      121 gaacttgccg tagtccatga gcttgacgac gggcggacgc gagttcgcgg ccacctcgac
      181 cagatcgaga tcggattcct gagcaagccg catggcatcc tcaatcctga cgatgcctac
      241 ctgctcgccc tgagggccta ccagtcggac ctcggggact cggatgcgtt cgttgatgcg
      301 gggctcgttg ataatcgctc cttcgtagtc gtgaatgttc tcccgacaag gaaaaaggcc
      361 cctgccggaa cgcgggcaga ggccaccaaa ggacgagatg aacgcgagcg tccatctaga
      421 ccaaaccctg gcccgctaaa gcggccctga gggtgggagg tgatctccac ttgcgagccg
      481 cggaacatcc acggccggtc gacttgcaag aatagcacca tgaactcccc cacgcccagc
      541 ccggactccc agaacccctc gcccagcctc ggcgacccgc tcgtcgccgc tcgcgacatc
      601 gccgacgtgt cggcgatcga actcatcact acggtgagtg tgcacctgat gtccgccgct
      661 gccctccgat gcgggctggc agatgagggg gaagagttcc aagacctttc ggaagccagg
      721 atcatgatca acgccttggc ggcgctcgtc accggggcct ctccagacct tggcgacacc
      781 cacgcgcgtg cgctccgaga cggcctcaga agcgtacagt tggcctttcg agaggcgtcc
      841 gtggtgcccg acgcccccgg ggagggtccc ggcgagaaat acaccggagc ggtgaactag
      901 atgaccacta ccccctcgca tgacaacggg aagatttcgc gcactgccgc gaacgaggaa
      961 accaccgtcc tcacgcccat gtcggatggc gccaccccgc ttgagcgcat gccggtcaaa
     1021 cgccagcgac tgtggatcat cgtcacttcc ctcctcgggg tactcctgat caccgcgggg
     1081 atcatcatct accgcctggt ggacgtgtcg aatctgtggg aagcccaggt cgaggacgtc
     1141 aaggcgcaga attacgacct cggcgagcgc ctggcggaag aacaggccca gggcatcgaa
     1201 cttcagggcc aggtggacct cgtcaacgaa caactccgca ccgcacagca acgcgttctt
     1261 gagctcgccg acgatgtcgc gcagcgcgac gacaatgccg agttctacgc gcgccaaatc
     1321 actgacctca ccgacgtcct caccacggca tcggcggtcg ccaattcgct caacaagtgc
     1381 gtggactacc accaacagct cgtcgacatc ctcaagcagc ccgacaacta cctcccggat
     1441 gaggtcgcca ccttcgaggc cggcgtcacc aaggtgtgcg acgcggccac ggctgccaat
     1501 gtcgacctgc agacggtgct tgcccaatga gatcaccccg cgcgctcctt gccgcgtgcg
     1561 ccgtcgcagg tctgctcgcc ggctgtgcgg cgcttcccga cgcgcccagc cccctgccgt
     1621 ccgactacgt gccggcggca cctgtggtcg acgtggcgca gcaaggaaac ctttcacccg
     1681 acggcttcag ccaggcacag cgcatgaccg tgagggtgcg aaacgtcgga tgcgactccc
     1741 tcaagacagg caccggcttc gcgtacgacg cccacacgct cattacgaac cgtcacgtgg
     1801 tggaggacac cagcgaactt caggtcacca cctacgacgg tcgcacgatc gcagtgacgg
     1861 ccgcttcggt ggccgccgtc gcggacttgg ccatcatcac cacggaggag tcgatcggcg
     1921 cgggcgcagt gctcgctcaa gaggaccccg tcgagggcga cgcgatcacg gtggtcggct
     1981 accccaatgg cggccaactc accaccgtgt cgggcgtggt gctcggtgcg actggagacc
     2041 ctctcggcgc ggccctgggc acggtgctcg cgacgacggc tccagtggag ccaggctcgt
     2101 ccggctcgcc cgtactcaac gaacgcggcg aggtcgtggg cgtcatctac gcgaagaacg
     2161 aagcggagca gtcgttcatg gtgccggtgt cactgctgcg cacgctgctc gacgaggatc
     2221 aactcctggt cccacagcag caatcctgca acccgtccta gaccagagtg atctttctcg
     2281 cacctcgtag caggaacatc ccgtagggcc ccccgctccg gtagtgtccg tggcgcaagc
     2341 ctgagggaga gccatgaccg agcagcccgg cgcgagcacg cccagaccgg cagagctcca
     2401 cccgacccac cgcagcacgc ggtgcttgcg acggggccgc gcgggctcat ggggcggggg
     2461 tgcgtcgttc gtggccggag cctccgcatc cggagcggcg tgctgatcaa tgtccggtgt
     2521 gggcgcgctc gcgccgggct gctcggtcat ggctctccct caggcttgcg cctaggacac
     2581 taccggagca tcgggttgcg cagagcgccg atgttggccc ggcgggcgag gtgcttcccg
     2641 tttaggac
//
LOCUS       DASRKH010000271         2347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_10786, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000271 DASRKH010000000
VERSION     DASRKH010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10786"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VFC06_04405"
     CDS             complement(<1..491)
                     /locus_tag="VFC06_04405"
                     /inference="COORDINATES: protein motif:HMM:NF015596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81168.1"
                     /translation="MTKLSRVSALAASLLLAGSIAACGGGDDGGASSTGSAATSSAET
                     SGAPTEAPTATATSDDTAAGQTVTVMTAETDLGTILVDGAGMTLYLFTNDTPGVSTCE
                     GGCLAAWPPLLGTPQAGEGADDSLLGTLQRSDGSTQVSYNGWPLYYWASDAVAGDTTG
                     QGV"
     gene            675..1250
                     /locus_tag="VFC06_04410"
     CDS             675..1250
                     /locus_tag="VFC06_04410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZL81169.1"
                     /translation="MQLFSQADRPADVALVAGLAVDDEPAAAAFVRRFQGPVFGLAVS
                     ITRDAALAQDVSQEVFVRAWRAASTYDVRRASVLTWLLTITHNAAIDAVRARRQTPTD
                     GAVLEEMIAATMQAPDPEEEAVRHAEWDAAVERLRALPPEQARAVVLAVIGGRTALEV
                     SEHEGIPLGTAKTRIRAGLRHVREAMEADRD"
     gene            1243..1890
                     /locus_tag="VFC06_04415"
     CDS             1243..1890
                     /locus_tag="VFC06_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81170.1"
                     /translation="MTDRHPDEVLLLDLALSDVDGHARDELTRHIALCEPCRAEYAAI
                     ADAVDHVLAAAPRVAPPPGFSRSVLAAMGMGEHRVARRRRRFALAAVASVSLLVGTVG
                     TLVVLESRQGEAVEVAAIGPALVTGDGTRVGTVLDSWYDGAPVLVVTLTDAPAGVRYT
                     CELVYADGTRFAAGSWSLDAAGSATWVVTRPDTDTELTGMELVTDAGTAWATATL"
     gene            1923..>2347
                     /locus_tag="VFC06_04420"
     CDS             1923..>2347
                     /locus_tag="VFC06_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZL81171.1"
                     /translation="MTSTSHGFVIVGASLAGAKAAETLRKEGFAGRVTLIGDELHRPY
                     ERPPLSKGYLLGSAERESVFVHPATWYAEHDVDLRLGTRATAVDARGHEVHLADGDPL
                     RYAALLLATGSEPVRLTIPGADLEGGLQRLAVLQPSQVA"
BASE COUNT          305 a          841 c          870 g          331 t
ORIGIN      
        1 ttgacgccct gaccggtggt gtcaccggcc actgcgtcgc tggcccagta gtacagcggc
       61 cagccgttgt aggagacctg cgtgctgccg tcgctgcgct ggagggtgcc gaggagtgag
      121 tcgtcggcgc cctctccggc ctggggtgtg ccgaggagcg gtggccaggc ggccaggcac
      181 ccgccctcgc aggtgctcac gccgggcgtg tcgttggtga acaggtacag cgtcatgccg
      241 gcgccgtcga cgaggatcgt gccgaggtcc gtttcggcgg tcatcaccgt gacggtctgg
      301 ccggcggctg tgtcatcgct ggtcgcggtg gcggtcggcg cttcggtcgg cgcgccggac
      361 gtttccgcgc tcgacgtggc cgcgctcccg gtcgacgacg caccgccgtc gtccccgccg
      421 ccacacgcgg cgatcgagcc ggccaggagc aggctggccg ccagcgccga cacgcgagat
      481 agtttcgtca tggttcctcc aggtgctcag ctgcgtcggc cgacccgggc gggacggtcc
      541 atgtggtgat acggctcaca cccccggagg gattgcccgc ggacgggtcg ccgtcgccca
      601 agccgtcgcc ggtgccgggc ggatcggacc ggcggcccgc aatccctgcc gccccggcgg
      661 acgtacaacc tgatgtgcag ttgttctcgc aggcggaccg gccggcggat gtcgcgctgg
      721 tcgcgggcct cgcggtcgac gacgagcccg cggccgccgc gttcgtgcgc cggttccagg
      781 ggccggtgtt cgggctggcg gtctcgatca cccgcgacgc ggccctcgcg caggacgtca
      841 gccaggaggt cttcgtgcgg gcctggcgcg ccgctagcac ctacgacgtc cggcgcgcat
      901 cggtgctgac ctggttgctg accatcaccc acaacgccgc gatcgacgcc gtccgcgccc
      961 gccgccagac acccacggac ggcgcggtgc tcgaggagat gattgccgcg accatgcagg
     1021 ccccggaccc ggaggaggaa gccgtgcggc acgccgaatg ggacgccgcc gtggagcggc
     1081 tgcgcgccct accgcccgag caggcacggg ccgtggtgct cgccgtcatc ggcgggcgta
     1141 ccgccctcga ggtcagcgag cacgagggca tcccgctcgg gactgccaag acgcggatcc
     1201 gtgccgggct gcgacacgtg cgcgaggcga tggaggcgga ccgtgactga ccggcacccc
     1261 gacgaggtac tcctgctgga cctggcgctc tccgacgtcg acgggcatgc acgcgacgag
     1321 ctcacccggc acatcgccct gtgcgagccg tgccgggcgg agtacgccgc gatcgccgat
     1381 gccgtcgacc acgtcctggc tgcggcgccc cgcgtcgccc cgccgcccgg cttctcacga
     1441 tccgtcctgg ccgcgatggg gatgggagag caccgggttg cgcggcgacg tcgtcggttc
     1501 gccctcgcgg cggtcgcgtc ggtttcgctg ctcgtcggca ccgtcggcac gctcgtggtc
     1561 ctggagtccc ggcagggcga ggcggtcgag gtcgcggcca tcggacctgc actggtcacg
     1621 ggtgacggca cgcgcgtcgg gacggtcctg gacagctggt acgacggcgc gccggtgctg
     1681 gtcgtcaccc tcacggacgc gccggcgggg gtgcggtaca cgtgcgagct cgtgtacgcc
     1741 gacggcaccc ggttcgccgc cggctcgtgg agcctcgacg ccgccgggag cgccacgtgg
     1801 gtggtcacgc gcccggacac ggacaccgaa ctgaccggca tggaactcgt gaccgacgcg
     1861 ggcacagcct gggcaacggc gacgctctga ttcaggcagc ggacacagaa cgggaggaac
     1921 cagtgacatc cacatcccac ggattcgtga tcgtcggggc gagcctggcg ggcgcgaagg
     1981 cggccgagac gctgcggaag gaggggttcg ccgggcgggt cacgctgatc ggcgatgaac
     2041 ttcaccgccc gtacgagcgg ccaccgctct cgaaggggta cctgctcggt tccgccgagc
     2101 gcgagagcgt cttcgtccat cccgccacgt ggtacgccga gcatgacgtc gacctgcgcc
     2161 tcggaacgcg ggccaccgcc gtcgacgccc gcggtcacga ggtccacctc gccgacggcg
     2221 acccgctgcg ctatgccgcg ctgctgctgg ccaccgggtc ggaaccggtg cggctgacga
     2281 tccccggtgc cgacctcgaa ggcggccttc agcgcctcgc tgtcctccag ccgtcgcagg
     2341 tagcgga
//
LOCUS       DASRKH010000272         4103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_23086, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000272 DASRKH010000000
VERSION     DASRKH010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23086"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1908
                     /locus_tag="VFC06_04425"
     CDS             <1..1908
                     /locus_tag="VFC06_04425"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZL81172.1"
                     /translation="SAGRATLDAARVDTASQLDHEVSTAHAGADEWQSLSPEDRAALL
                     HAAGDALESHRADLIEVMMSEAGKTIDQADPEVSEAVDFAHYYAERSLDLWTMTGAVP
                     VPRHVTLVTPPWNFPVAIPAGSTLAALAAGSAVILKPAAQSARCGAVLAEALWEAGIP
                     RDALRLIKIDSKVLGEPLITDERIDQVILTGGYDTAERFLTMRPNLRLFAETSGKNSI
                     IVTPSADVDLAVRDVVASAFGHAGQKCSAASLVIAVGSVATSRRFFTQLEDAVTSLVA
                     GPAHKATTQMGPVIEPVHGKLERALATLEPGERWLVEPRDLDGTGTAWTPGLKTGVAP
                     GSFFHQTECFGPVLGIMVAATLDEAMALQNAVDYGLTAGLHSLDKAEIERWLATVQAG
                     NAYVNRGITGAIVRRQPFGGWKRSVVGCTYKAGGPHYLQALTDWEARASDAAAEGAPG
                     DRLEAFLECAEAPKWVRNAAAADAHAWKTTFGTATDRTGLASEANALRYAPADTDIRW
                     DGVASVDSLVRVCAARVRAGGAGVVSTPVPLPAELAEALAAQGVGVRVEPWDACVSRA
                     AARLGGRVRLVTGRDSEAFGTAQAAVFTQPVTGNPELELLPFVHEQALSVTTHRFGTP
                     FDVAREVVAAL"
     gene            complement(1915..2667)
                     /locus_tag="VFC06_04430"
     CDS             complement(1915..2667)
                     /locus_tag="VFC06_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HZL81173.1"
                     /translation="MTGSRRPIIGVSSYLERAVMDVWDVPAVFLPTSYIVPIQRAGAS
                     IVVLPPQDTDPAGAAAIVAGLDGLCISGGYDVDPTTFAQTPHPETDQPRPQRDAWEFA
                     LIAAAQGSDMPILGVCRGAQVLNVARGGTLVQHVPEVVGHGGYQGSDGVFVTMPISTT
                     PGSLLARLHPARRDVPVYHHQAIDQLGDGLVVSAWGDDGVIQAVEDASLTFCVATQWH
                     PEQDPGSARLYEAFVDAARKFNELGSAARQRN"
     gene            complement(2664..3431)
                     /locus_tag="VFC06_04435"
     CDS             complement(2664..3431)
                     /locus_tag="VFC06_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020015431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HZL81174.1"
                     /translation="MSSHPYFDTGGTFAALAHRGFSLDGLENSMVAFQAAVDMGYRYV
                     ETDAHGTIDGVAVALHDESLDRTTDGTGEVADLPWARVKLARIGGKEPVPMLEDILGT
                     WPELRVNIDVKAESGIVPVADAIEKTKAHDRVCIASFSSARRDATVARLTRRVATSAG
                     TREAAAFFLGGMVRANLAGALRSVDALQIPASMGPIPLLTERHVRAAHAAGKLVHVWT
                     INDPQRMAQLIDLGVDGVVSDRADLLKDVLVAQGRWE"
     gene            complement(3428..>4103)
                     /locus_tag="VFC06_04440"
     CDS             complement(3428..>4103)
                     /locus_tag="VFC06_04440"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZL81175.1"
                     /translation="YMTDVSEQGDGIDLALAAITALHPGLPIVVHGHSTGGLAAAIWA
                     SERPHASLVGLILDSPLFGTTLTRTQKARVLALPLLARIRPMAVVASAPSIYAQHQHA
                     SGGGRWQFDFALKRPAGVPIRAAWLAAARRARAKVARGLDVRLPVLVAMSDSSGPDVA
                     DNPLLDRQDTVLSVDEIVRLGPRLGDDVEVVVIPGGVHELSLSQDGPRELYLRTVTRW
                     LDSVIA"
BASE COUNT          687 a         1353 c         1432 g          631 t
ORIGIN      
        1 tcagccggtc gagccaccct cgacgccgcg cgcgtcgaca ccgcatcaca actcgaccat
       61 gaggtctcga ccgctcacgc cggggccgac gaatggcaat cgctcagccc cgaggaccgg
      121 gccgccttgc ttcacgccgc cggcgacgcg ctggagtcac accgcgccga cctcatcgag
      181 gtgatgatgt cggaggcagg caagaccatc gaccaggcag acccggaggt ttcggaagcc
      241 gtcgacttcg cgcactacta cgcggagcga tcccttgatc tgtggacgat gaccggagca
      301 gtgccggtac cgcgccacgt caccctggtg acgccgccgt ggaattttcc cgtggcgatc
      361 ccggcagggt ccacgttagc cgcgctggcg gcgggttcgg cggtcatcct caagcccgcg
      421 gcgcagtcag cgcgatgcgg ggctgtcctt gccgaggcat tgtgggaggc ggggattccg
      481 cgcgacgcac tccggctgat caagatcgac tccaaggtcc tgggagagcc actcatcacc
      541 gacgaacgga tcgaccaagt catcctgacg ggcggctacg acaccgccga gcggttcttg
      601 acgatgcggc cgaatctacg cctgtttgcc gagacgagcg gcaagaactc gatcatcgtg
      661 accccgtctg ccgacgtcga cttggcggtc agggacgtgg tggcatcggc gtttggtcac
      721 gccgggcaga agtgctcggc ggcgtcgctg gtgatcgcgg tcggatccgt cgcgacgtcg
      781 cgccgcttct tcacccagct tgaggacgcg gtgacatccc ttgtggcggg gccggcgcac
      841 aaggccacca cgcagatggg gcccgtcatc gagcccgttc acggcaagtt ggagcgagca
      901 ctcgcgacgc tggagccagg ggagaggtgg ctcgtcgagc cccgcgacct cgacggcacg
      961 gggacggctt ggaccccggg gctaaagacc ggagtcgccc ctggttcctt cttccaccag
     1021 accgagtgct tcggcccggt gctcggcatc atggtggctg cgacgctcga cgaggcgatg
     1081 gccctgcaga acgcggtcga ctacggactc acggcgggac tccactcgct ggataaggcg
     1141 gagattgaac ggtggttggc gaccgtccag gccggcaacg cctacgtcaa caggggaatc
     1201 acgggggcca tcgtgcggcg acaaccgttc ggcgggtgga agcgatccgt cgtgggctgc
     1261 acgtacaagg ctggtgggcc gcactatctc caagccctca ccgattggga ggcccgtgcc
     1321 tcagacgccg ccgcagaagg cgccccagga gaccgtctgg aggccttcct ggagtgcgcc
     1381 gaggcgccca agtgggtgcg gaacgcggcg gccgccgatg cacacgcttg gaagaccacg
     1441 ttcggcacgg cgaccgaccg gacgggtctc gcctccgagg cgaacgctct gcgttacgcg
     1501 cccgcagaca cggacatccg ctgggatggc gtcgcgtccg tggactcgct cgtcagggtg
     1561 tgtgcggctc gcgtgagggc gggcggagcc ggagtggtct caacccccgt cccgctcccg
     1621 gccgagttgg ccgaggccct ggccgcccaa ggtgtgggcg tgcgagtaga gccatgggac
     1681 gcatgcgtta gccgtgcagc cgcacgtctg ggcgggcgag tcaggcttgt gaccggtcgc
     1741 gacagcgagg cgtttggaac tgcccaggct gcggtgttca cgcaacccgt caccggcaac
     1801 cccgaacttg agttgctgcc gttcgtacac gagcaggcct tgtcggtgac gactcaccga
     1861 ttcgggactc cgttcgacgt ggcacgcgag gtggtagcgg cgctgtaggg cggactagtt
     1921 gcgctgcctg gctgcggagc ccagttcgtt gaacttgcgt gccgcatcga caaaggcctc
     1981 atacaggcgc gctgaacccg ggtcctgttc ggggtgccac tgggtagcca cgcagaaggt
     2041 gagcgaggcg tcctcgaccg cttggatcac accgtcatcg ccccaagccg agacaaccag
     2101 cccgtcgccc aactggtcta tggcttgatg gtggtagacg gggacgtcgc gtcgtgcggg
     2161 atgaagtcgc gccaggaggc taccgggcgt ggtcgagatg ggcatcgtca cgaacacgcc
     2221 gtcgctgcct tgatatccgc cgtggcccac gacctcgggg acgtgttgaa ccagagtgcc
     2281 gcctctcgcg acattgagca cttgcgcgcc ccggcacacg ccaagaattg gcatgtcgct
     2341 accctgcgcg gccgcgatga gcgcgaactc ccaggcgtcg cgttgggggc gaggctggtc
     2401 ggtctcgggg tggggcgtct gggcgaaggt ggtggggtcg acgtcgtagc ctcccgagat
     2461 gcacaggccg tcgaggcccg ccacgatcgc agccgcgcca gccggatccg tgtcctgcgg
     2521 cggcaagacg acgatggagg cccccgcccg ctgaatcggc acgatgtacg acgtcgggag
     2581 gaacacggcg ggcacgtccc acacgtccat gacggcccgc tccagatagc tgctgacgcc
     2641 gatgatgggg cgtcgagacc cggtcattcc cacctgccct gggccacgag cacgtccttc
     2701 agcaggtccg cgcggtcgga cacgacgccg tcgactccca ggtcaatcaa ctgcgccatc
     2761 cgctggggat cgttgatggt ccacacgtgc acgagtttcc ctgccgcgtg ggcggcgcgc
     2821 acgtgccgct cggtgagaag cggaatcggt cccatcgacg ccggaatctg aagtgcgtcg
     2881 accgacctga gcgcgcccgc gagattcgca cgcaccattc cccccaagaa gaaggcggcg
     2941 gcttcgcgag ttcccgccga tgtcgcgacg cggcgggtca ggcgcgctac cgtcgcgtcg
     3001 cgccgggccg aggaaaacga ggcgatgcac acgcgatcgt gcgccttggt cttctcgatc
     3061 gcatccgcca cggggacgat gccggactcc gccttcacgt cgatgttgac gcgtagctct
     3121 ggccacgtgc cgaggatgtc ttcgagcatt ggaacgggct ctttgccccc gatgcgcgcg
     3181 agcttcaccc gggcccaggg caagtccgcg acctcgcccg tgccatccgt cgtgcggtcg
     3241 agggactcgt cgtgcagtgc gaccgcaacc ccgtcgatcg ttccgtgagc gtccgtttcg
     3301 acgtagcggt aacccatgtc caccgcggcc tggaaagcga ccatcgagtt ctcgaggccg
     3361 tcaagcgaga agccgcggtg cgcgagcgcg gcgaaggtgc cgccggtgtc aaagtaggga
     3421 tgcgagctca tgcgatcaca ctatccaacc agcgggtgac ggtgcgcaga tacaactcac
     3481 gcggcccgtc ctgtgacagc gacaactcgt ggacgccgcc gggaatcacc accacctcca
     3541 cgtcgtcccc gagccggggc cctaagcgga cgatctcgtc gacgctgagg actgtgtctt
     3601 gacgatcaag cagggggttg tcggccacgt ccggaccgga ggaatcggac attgcgacca
     3661 agacgggcag tcgcacatcc aggccgcgcg cgaccttggc ccgggcccgc cgtgctgcgg
     3721 ccagccacgc cgcacgaatg ggcacgccgg cgggtcgctt gagggcgaag tcgaactgcc
     3781 atcggccgcc gccggaggcg tgctggtgtt gggcgtagat cgagggcgcg gacgcgacca
     3841 cggccatcgg tcggatcctg gccagcagcg gcagggcgag cacgcgcgcc ttctgggtac
     3901 gcgtgagggt ggtgccaaac aggggggagt caagaatcag gccgacgagc gacgcgtggg
     3961 ggcgctcgga cgcccagatt gccgcagcca agccgccggt cgagtgccca tgaaccacaa
     4021 tcggcaggcc ggggtgcaaa gcggtgattg ccgcgagggc aaggtcaatg ccgtcgccct
     4081 gctccgacac atcggtcatg tag
//
LOCUS       DASRKH010000273         2015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_11856, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000273 DASRKH010000000
VERSION     DASRKH010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11856"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="VFC06_04445"
     CDS             complement(<1..837)
                     /locus_tag="VFC06_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HZL81176.1"
                     /translation="MTDLLSSPRPGAVDELNDALTDTELARSRAETDVLLADHGVTYG
                     TDDAAPQAPGWHLDPVPVIIDELEWTSLDAALVERAQVLDEVLIDLYGPRRLLTDALL
                     PPEIVLAHPGFLRAVDGLRLPAPRELVLTATDLTRDASGGWVAIADRTQAPSGAGYAM
                     EDRRVVARVRSGIYRQASIQRLGPFFHALKLALQDAAPAGATSPRVVLLTSGPHSETA
                     FDQSYLAAMLGLPMVEGSDLLVREGRVWMQGLDALEPVDVILRRVDADYCDPLELRAD
                     SRL"
     gene            complement(954..>2015)
                     /locus_tag="VFC06_04450"
     CDS             complement(954..>2015)
                     /locus_tag="VFC06_04450"
                     /inference="COORDINATES: protein motif:HMM:NF039189.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5671 domain-containing protein"
                     /protein_id="HZL81177.1"
                     /translation="GTPTITVAQAAATVIVGVPVWVVYWLRTWSTADRTPLWFGYVLP
                     VGVGASLVLAVVGASVAVYQVLVWLLGDPSSTQASQHFEGTPTAVACVAVGALSWWYH
                     RQVLAGAATGRTEVTRVYEYLMAGIALLAAAVGVTLVVVALIESLIPVADVELGTSVV
                     NSLLSGVTLLLVGSPLWWVFWSRINRVVHADGPEELGSPTRRTYLFVLFGLGGVAAVI
                     AVLVAAFVAIQGALQGNLDAQVVRDLRIPVAILLAAGAISGYHWAVYRDDRSRLPTPE
                     PRFGARYVLLVGAPDPAVRGAVEHRTGAQVDLWVRADGLAGPWVVDDVVAAVQGSPAP
                     AVTLVAGPAGLETVWMLRP"
BASE COUNT          309 a          715 c          776 g          215 t
ORIGIN      
        1 gagacgcgag tcggcccgca gctcgagcgg gtcgcagtag tcggcgtcga cgcgccgcag
       61 gatgacgtcg accggctcga gcgcgtcgag cccctgcatc cagacgcggc cctcgcgtac
      121 gagcaggtcg ctgccctcga ccatgggcag gccgagcatc gcggccaggt acgactggtc
      181 gaacgcggtc tcgctgtgcg gaccggacgt gagcagcacg acgcgcggcg acgtggctcc
      241 cgcgggggcg gcgtcctgca gcgcgagctt gagcgcgtgg aagaacggcc cgagccgttg
      301 gatcgaggcc tgccggtaga tcccggaacg cacgcgcgcg acgacgcggc ggtcctccat
      361 cgcgtacccc gcgcccgacg gcgcctgcgt gcggtcggcg atcgcgaccc agccgcccga
      421 cgcgtcgcgc gtgaggtcgg tggccgtcag gacgagctcg cggggcgcgg gcagccgcag
      481 cccgtcgacc gcacgcagga acccggggtg cgcgagcacg atctcgggcg gcaggagcgc
      541 gtccgtgagc aggcgacgcg ggccgtacag gtcgatgagg acctcgtcga gcacctgtgc
      601 ccgctcgacg agggccgcgt ccagcgacgt ccactcgagc tcgtcgatga tcaccgggac
      661 ggggtcgagg tgccagccgg gcgcctgtgg agcggcgtcg tcggtgccgt acgtgacacc
      721 gtggtcggcg agcagcacgt ccgtctcggc tcggctgcgg gcgagctcgg tgtcggtcag
      781 cgcgtcgttg agctcgtcga ccgcgccggg acgcggcgag gacagcaggt ccgtcactcg
      841 ggctccctcc ggcgacatgg cccgcgccgg gtggcgcgcg ccttggggga ccaccccatc
      901 acgccggggg ctggacccga tgcaccgacc acgtgtcggg cacgggttcg tggctacggg
      961 cgcagcatcc agaccgtctc gagccctgcc ggccccgcca caagcgtgac cgcgggggcc
     1021 ggggacccct ggaccgcggc gaccacgtcg tcgaccaccc agggcccggc caggccgtcg
     1081 gcgcgcaccc acaggtcgac ctgggcgccc gtgcggtgct cgaccgcgcc gcggacggcg
     1141 gggtcggggg cgccgacgag cagcacgtac cgcgcgccga accgaggctc gggcgtcggc
     1201 agccgcgagc ggtcgtcccg gtagacggcc cagtggtagc cggagatcgc gccggcagcc
     1261 agcaggatcg cgacggggat gcgcaggtcg cggaccacct gggcgtcgag gttgccctgc
     1321 agggcgccct ggatcgcgac gaacgcggcg accaggacgg cgatgaccgc agcgaccccg
     1381 ccgaggccga agagcacgaa gaggtaggtc cgacgggtcg gcgaaccgag ctcctccggg
     1441 ccgtcggcgt gcacgacccg gttgatgcgc gaccagaaca cccaccacaa cgggctaccg
     1501 acgaggagga gcgtcacgcc ggaaaggagc gagttgacca ccgaggtgcc gagctcgacg
     1561 tcggcgaccg ggatcagcga ctcgatgagc gcgaccacca ccagggtgac gccgaccgcg
     1621 gcggccagca gggcgatccc cgccatcagg tactcgtaga cgcgggtgac ctcggtgcgt
     1681 ccggttgccg cgccggccag gacctgccgg tggtaccacc agctgagagc accgacggcc
     1741 acgcaggcga cggcggtcgg ggtgccttcg aagtgctggg aggcctgggt ggacgacggg
     1801 tcgccgagga gccagacgag gacctggtac accgcgacgc tggcgccgac gacggccagg
     1861 accagcgagg ctccgacacc gaccgggagc acgtagccga accacagcgg cgtccggtcc
     1921 gccgtggacc aggtccgcag ccagtagacg acccacacgg ggacgcccac gatcaccgtc
     1981 gcggcggcct gcgcgacggt gatcgtgggc gtccc
//
LOCUS       DASRKH010000274         4029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_44550, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000274 DASRKH010000000
VERSION     DASRKH010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44550"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="VFC06_04455"
     CDS             complement(<1..614)
                     /locus_tag="VFC06_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81178.1"
                     /translation="MLQGLGIRGKLLAVVAFPTIVLLLAASFVVFNAVTDQSAARNTD
                     QLVTLVQDGAAMVQDIQAERGDVLNYLSATSDGLDKSSTAGTAVDNTAATITAAAAED
                     PAYAAAANAISAAVSGAGGEDGLDTYREITTVPAVEGDFAAWPEPAAVATAQAGFIRI
                     ADEVEAAAVGAPVSDTGNVIRGLADLIRTEGVEIATYLNESKQYA"
     gene            792..1754
                     /locus_tag="VFC06_04460"
     CDS             792..1754
                     /locus_tag="VFC06_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HZL81179.1"
                     /translation="MRAIVVGSGQAAGELSVRDVAAPQAGPGEVVISIAAAGVNRADL
                     LQRKGQYPSPPGWPEWPGLECSGIVAQVGKGVDDVHEGDAVCALVGGGAYADAIAVPA
                     DLMLPVPHGLDLIAAGGLMEAACTVWSNLATAAVTPGETLLVHGGAGGIGSFAIQIGK
                     AMGLRVIATAGGPDRAQWCRDIGADIAIDYRREDFVAATQAVGGADVILDVVGAGYLN
                     RNVDALATGGRLVVIGLLQGATAPLDLGALMARRLRVIGTTLRSRPHAERAAIVAAVG
                     REVWPWIPSKVSPMLHAAIPLEEASRAHDMLADGEVAGKIVLTP"
     gene            complement(1761..2993)
                     /locus_tag="VFC06_04465"
     CDS             complement(1761..2993)
                     /locus_tag="VFC06_04465"
                     /EC_number="5.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HZL81180.1"
                     /translation="MNLLEDHAHRAWLEAEADRLLAFGRASRSAIGGFGWLDDDGATD
                     PSHPVELWISCRMTHSYALSVLRGNAADRALVNHGVASLRGALRDDANGGWFASIGPD
                     GPVNDAKETYGHAFVILAASSAAATGHPEAAALLDEALAVFDEHFWDDEQGLAREAYA
                     ADWSGLSDYRGVNANMHTVEAYLAAADVAARPDLLERALRITTRVVNEWARNNQWRLP
                     EHFTPEWEPLLEYNRDEPAHPFRPYGATIGHLLEWSRLTLQLEASLLRAGLDAPRWMV
                     GAARELYDAAVREGWNVDGAPGFVYTIDWNGVPVVRDRMHWVTAEAIGAALTLWLREG
                     DEATAADYEVWWNYAREHHLDLVGGSWWHELAPDNTVGHSVWEGKPDIYHALQATLFP
                     RLPITPSLASALGAGLLQ"
     gene            complement(3021..3875)
                     /locus_tag="VFC06_04470"
     CDS             complement(3021..3875)
                     /locus_tag="VFC06_04470"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020015664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZL81181.1"
                     /translation="MAVDPEQLDPPLTPDDWRLVGLDIDGTLMHWGGEITDAVVDAVT
                     KVRMSRNHVVLATGRNIVATMPVAERLSMRRGWAVCSNGAVIIRLNPGSPGGYDITKT
                     ITFNPREALEVIRDEMPDAFFAVEDLGVGFRVTKEFPMGELAGRQRTVTFEELCHDDV
                     TRVVIRAPGVHVDHFEDLVHRIGLSDVTYAVGYSAWLDLTPPGVSKASALETLREQLG
                     VHPEHTVAVGDGNNDIEMLKWARDSYAMGNAPERVIAAAKSEIGPVDEDGVLDALAPL
                     IDPARLAF"
BASE COUNT          653 a         1356 c         1347 g          673 t
ORIGIN      
        1 gcgtattgct tggattcgtt gaggtacgtg gcaatctcaa cgccctcagt cctgatgagg
       61 tcggcgagcc cacggatcac gtttccggtg tcgctcacgg gggcgcccac ggcagcggct
      121 tccacctcgt ccgcgatgcg aatgaatccg gcctgcgcag ttgccacggc ggcgggctcc
      181 ggccaagcgg caaaatcgcc ttcgacggca gggaccgtcg tgatctcacg gtaggtgtcc
      241 agaccgtcct caccacctgc accgctcacg gcggcggaga tggcattcgc cgcggcggca
      301 taggcggggt cctcagcggc cgccgccgtg atcgtcgcag cagtattgtc aacggccgta
      361 ccggcggttg agctcttgtc tagaccgtcg ctcgtcgcac tgaggtagtt caacacatcc
      421 ccgcgctcgg cttggatgtc ctggaccatg gcggcgccgt cctggaccag cgtcaccaac
      481 tggtcggtgt tgcgcgcggc agactggtcg gtgacggcat tgaacaccac aaaggaggcg
      541 gcaagaagaa gaacgatcgt cgggaaggcg acgaccgcga gcagcttgcc acgaatacct
      601 aacccttgga gcatgtacct ctccctcgtg cccgtcagcc gtatgccgac gggtcgccag
      661 ccacaccgcc gacgcgtgtg atcctcagta atatcggcac tttgccgtga tcacatgagg
      721 tcgccccacc gcggcccgca ccatgagacg gtataacgga tcagttatga cgtgaaaggg
      781 gcaaattgcc tatgcgtgcg atcgttgtcg gctcgggtca agccgccggg gagctgtcgg
      841 tacgggacgt cgccgcaccc caggccggtc cgggcgaagt ggtgatctcg atcgcggctg
      901 caggggtcaa ccgcgccgac ctgctccagc gcaagggcca atacccctcg ccccccggct
      961 ggcccgagtg gccaggtctc gaatgctcgg gaatcgtcgc ccaggtgggc aagggcgttg
     1021 acgacgtgca cgagggtgat gccgtctgcg cccttgtcgg aggcggcgcc tacgccgacg
     1081 ccatagccgt tcccgccgat ctcatgcttc cggtgccgca cggcctcgat ctgattgcgg
     1141 cgggcgggtt gatggaggcc gcgtgcacgg tgtggtcaaa cctggccacc gcagcggtca
     1201 cgccgggcga gaccctgctc gttcatggcg gtgctggcgg gatcgggtcg ttcgcgatcc
     1261 agatcggcaa ggcgatgggg ctccgcgtga tcgcgaccgc cgggggcccg gacagggcac
     1321 agtggtgtcg ggatatcggc gccgatatcg cgatcgacta tcggcgcgag gacttcgtgg
     1381 cggcaacgca ggccgtcggt ggcgctgatg tgatcctcga cgtcgtcggc gctggctatc
     1441 tgaatcggaa cgtggacgcg ctggccacgg gaggacgcct ggtcgtgatc gggctgcttc
     1501 agggcgccac cgcgcccctt gacttgggcg cgctgatggc gaggcgactg cgcgtgatcg
     1561 gcaccacgct acgctcgcgg ccccacgcgg aacgcgcagc gatcgtggcg gcggttggcc
     1621 gagaagtgtg gccctggatt ccgtctaagg tgtctccgat gctacacgca gcgatcccgc
     1681 ttgaggaggc gtcacgcgcc catgacatgc tcgccgacgg cgaggtcgcg ggcaagatcg
     1741 tcctcacccc gtagcgcgcg ctactgaagc agccccgccc cgagcgcact tgccagcgac
     1801 ggcgtgatcg gcagtcgcgg gaagagcgtg gcctgaaggg cgtggtagat gtccggcttg
     1861 ccttcccaca cggagtgacc gacggtgttg tcgggggcca actcatgcca ccatgacccg
     1921 ccgacgaggt cgaggtggtg ctccctcgcg tagttccacc agacctcata gtcggccgcg
     1981 gtggcctcgt cgccctcgcg gagccatagg gtgagcgccg cgccgatggc ctccgccgtc
     2041 acccagtgca tccggtcccg gaccacgggc acgccgttcc agtcgatcgt gtagacgaag
     2101 ccgggtgcac cgtcgacgtt ccacccttcg cgcaccgcag cgtcgtacag ctcgcgggcc
     2161 gcgcccacca tccagcgagg cgcatcgagg cccgcgcgca acaacgatgc ctcgagctgg
     2221 agcgtcagcc tcgaccactc caagaggtgg ccaatcgtcg cgccgtaggg acggaaggga
     2281 tgcgcaggct cgtcgcggtt gtactccagc agcggctccc actccggcgt gaagtgttcc
     2341 ggcaggcgcc actgattgtt tctggcccac tcgttgacca cccgggtggt gatccgcaga
     2401 gcgcgctcca acaggtcggg gcgggcggcg acgtcggccg ccgcgaggta cgcctcgacg
     2461 gtgtgcatgt tggcgttgac gccccggtag tcggacagtc cagaccagtc cgccgcgtag
     2521 gcctcgcggg caagaccctg ttcgtcgtcc cagaagtgct catcgaacac ggcgagcgcc
     2581 tcgtcaagaa gcgccgccgc ctccgggtgc ccagtcgccg cggccgagga tgccgccagg
     2641 atcacgaagg cgtgcccgta cgtctccttg gcatcgttca cgggaccgtc gggcccgatg
     2701 ctcgcgaacc aaccgccgtt cgcgtcgtcc ctcagcgcgc ctcgcaagga cgccactccg
     2761 tgattgacga gcgcccgatc ggccgcgttt ccccgcagca ccgacagcgc ataactgtgc
     2821 gtcatccggc aactgatcca cagctcgacg gggtgggagg ggtcggtcgc tccgtcgtcg
     2881 tcgagccatc cgaagccgcc gatggcagaa cggctcgcgc gtccaaacgc gaggagacgg
     2941 tcggcctcgg cttcaagcca ggcccgatgt gcgtggtctt ccaggaggtt cataggtcca
     3001 acctacggcg tgcgggaccg ctagaaggcg agccgggcag ggtcgatcag tggtgcgagg
     3061 gcgtccaaca caccatcttc atccaccggt ccgatctcgc tctttgcggc ggcgatgacg
     3121 cgctccggcg cgttgcccat cgcgtaggag tcgcgcgccc acttcaacat ctcgatgtcg
     3181 ttgttgccat cgccgaccgc cacggtgtgc tcgggatgca cgccaagttg ctctcgcaag
     3241 gtctcgagcg ccgacgcctt ggacacgcct ggcggcgtga ggtccagcca cgccgagtag
     3301 ccgacggcat aggtcacatc gctcagcccg atgcggtgga cgaggtcctc gaagtgatcc
     3361 acgtgaactc ccggcgccct gatgaccacc ctggtgacgt cgtcgtggca caactcttca
     3421 aacgtgacgg tgcgctgccg ccccgcgagc tcgcccatcg gaaactcctt cgtcaccctg
     3481 aagccgactc ccaagtcctc gaccgcgaag aaggcatccg gcatctcgtc ccgaatcact
     3541 tccagcgcct cgcgcgggtt gaacgtgatg gtcttcgtga tgtcgtagcc gcctggcgat
     3601 cccgggttga ggcggatgat gacggcgccg ttggagcaca ccgcccagcc gcgtcgcatg
     3661 ctcaggcgtt ccgcgacggg catcgtcgcg acgatgttgc ggccggtggc gagcaccaca
     3721 tggttgcggg acatgcgcac cttggtcacc gcgtctacga ctgcatcggt gatctcgccg
     3781 ccccagtgca tcagcgtgcc gtcgatatcg aggccgacga gccgccagtc gtccggcgtg
     3841 agcggcgggt cgagttgttc cggatcaacg gccatgggtc gactttacga cgcgggcccg
     3901 caccgccagg tcacaccacc ggcgtcagca gtttcagacc acccaggtac ggacgcaaca
     3961 tcggggggac gtaaacgctg ccgtcggcct gctgatgatt ctccagcagt gcgacgatcc
     4021 aacgggtgg
//
LOCUS       DASRKH010000275         2085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_17807, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000275 DASRKH010000000
VERSION     DASRKH010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17807"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VFC06_04475"
     CDS             complement(<1..352)
                     /locus_tag="VFC06_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HZL81182.1"
                     /translation="MTHRLELLPAVDVAGGQAVRLVQGEAGSETGYGDPLAAALDWQV
                     GGAEWIHLVDLDAAFGRGSNAALLADVVSQLNVKVELSGGIRDDASLESALATGCRRV
                     NLGTAALENPTWTAE"
     gene            complement(469..1044)
                     /gene="hisH"
                     /locus_tag="VFC06_04480"
     CDS             complement(469..1044)
                     /gene="hisH"
                     /locus_tag="VFC06_04480"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HZL81183.1"
                     /translation="MPSQPRVVVLDYGFGNVRSAVRSLERVGAFAAVMAGLRGVRGDQ
                     VIERRLAGGRPVLGICVGMQIMFAAGDEHGVHTEGLGEWPGLVDRLEAPVVPHMGWAT
                     VDAAEGSVLFAGVEDQRFYFVHSYAARSFPLSDTVDRERFAAPLVTWADHGGPFVAAV
                     ENGPLAATQFHPEKSGDAGAQLLTNWVTSLR"
     gene            complement(1054..1632)
                     /locus_tag="VFC06_04485"
     CDS             complement(1054..1632)
                     /locus_tag="VFC06_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81184.1"
                     /translation="MSGDVSASMPEDALGDDVVPQVEGLPDDLTIPDDLSSLTEPVDS
                     SLTESADDAVTVALVVTQVAAAGPLAAACSLAKVDVDVVPSPVGALALLRDPRTAAEG
                     AAAISRLLKTVPVVLLERRASQITATRWTAGEQCDELPPGLVLSDAPAVLEDLLLGGV
                     SVGDLDGVVTSVGLSRWKAMRMLASSTRGPRR"
     gene            1659..>2085
                     /locus_tag="VFC06_04490"
     CDS             1659..>2085
                     /locus_tag="VFC06_04490"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HZL81185.1"
                     /translation="MNPGVELDLDGTGITDISTTVGFYDHMLTAFGKHSLIDLTVRAT
                     GDTQIDVHHTVEDVAIAIGEALRQALGDKVGISRFGDALVPLDEALAQAVVDVSGRPY
                     LVHSGEPAGQEYHLIGGHFTGSLTRHVFEAIAYHAGICLH"
BASE COUNT          306 a          753 c          750 g          276 t
ORIGIN      
        1 cctccgccgt ccaggtcggg ttctccaagg cggcggtgcc caggttgacc cgccggcagc
       61 cggtcgccag ggcactctcg aggctggcgt cgtcccggat accgcctgag agctcgacct
      121 tgacgttgag ctgagacacg acgtcggcga gcagcgcggc gttcgaaccg cgaccgaagg
      181 cggcgtcgag gtcgaccagg tggatccact cggcaccgcc gacctgccag tcgagggctg
      241 cggcgagcgg gtcgccgtag ccggtctccg agccggcctc gccctggacg aggcgaacgg
      301 cctgcccacc ggcgacgtcg acggcgggca acagctcgag gcggtgggtc atgacgtcct
      361 ctctccgggc gacgtaccgg tcgtcgccgc tcgcgtgagc cgcggaaggg ccctgcgcag
      421 atcgtgtggg gtggtcaggt cgtgcttcag gtcgtgactc aggcgttgtc agcgcaacga
      481 cgtcacccag ttggtcagga gctgggcacc ggcgtcgccg gacttctcgg ggtggaactg
      541 ggtcgctgcg agcggaccgt tctcgactgc cgcgacgaac gggccaccgt ggtccgccca
      601 ggtcacgagg ggcgccgcga agcgctcccg gtcgaccgtg tcggacaacg ggaacgagcg
      661 cgccgcgtag gagtgcacga agtagaagcg ctggtcctcg acgcccgcga agagcaccga
      721 gccctcggcg gcgtccacgg tcgcccagcc catgtgcggc acgacgggtg cctcgagccg
      781 gtcgacgagg ccgggccact cccccaggcc ctcggtgtgc acgccgtgct cgtcacccgc
      841 ggcgaacatg atctgcatgc cgacgcagat gccgagcacc gggcgtccgc cggcgagacg
      901 ccgctcgatc acctggtccc cgcgcacgcc acgcaggccg gccatcacgg cggcgaaggc
      961 gccgacgcgc tcgagcgagc gcacggccga acggacgttg ccgaagccgt agtcgaggac
     1021 gacgacgcgg ggctggctgg gcacgggtcc aggctaccgg cgcggcccgc gggtcgacga
     1081 cgcgagcatc cgcatcgcct tccaccggga gagcccgacg ctggtgacga caccgtcgag
     1141 gtcgccgacg ctcacgccac ccaggagcag gtcctcgagg accgcgggcg catcggagag
     1201 cacgagcccg ggcggcagct cgtcgcactg ctcgccggcc gtccaccggg tcgcggtgat
     1261 ctggctcgct cgccgctcga gcagcacgac cggcacggtc ttgagcagtc gcgagatcgc
     1321 cgctgctccc tctgcggccg tccgcgggtc gcgcaggagc gcgagcgcgc ccaccggcga
     1381 cggcacgacg tcgacgtcga ccttggccag ggagcatgcc gccgcgagag gccccgcggc
     1441 cgcgacctgc gtgacgacga gcgcgacggt gaccgcgtcg tcggccgact ccgtgagcga
     1501 ggagtcgacc ggttcggtga gcgaggacag gtcgtccggg atcgtcaggt cgtcgggcag
     1561 tccctccacc tgcggcacga cgtcgtcccc gagcgcgtcc tcgggcatcg acgcactgac
     1621 gtccccgctc acaggaggcc cttggtgctc gggatcccct gaacccgggg gtcgagctcg
     1681 acctcgatgg gaccggcatc accgacatct ccacgacggt cggtttctac gaccacatgc
     1741 tcacggcgtt cggcaagcac tcgctcatcg acctcacggt ccgggcgacg ggcgacacgc
     1801 agatcgacgt ccaccacacg gtcgaggacg tcgcgatcgc gatcggcgag gcgctgcgcc
     1861 aggccctggg cgacaaggtg ggcatctccc ggttcggtga cgcgctcgtc ccgctcgacg
     1921 aggcgctcgc gcaggccgtc gtcgacgtgt cggggcgtcc gtacctcgtg cactcgggcg
     1981 agcccgcggg gcaggagtac cacctcatcg gtgggcactt caccgggtcg ctgacgcgtc
     2041 acgtcttcga ggcgatcgcc taccacgccg gcatctgcct ccacg
//
LOCUS       DASRKH010000276         4991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_28334, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000276 DASRKH010000000
VERSION     DASRKH010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28334"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..1718)
                     /locus_tag="VFC06_04495"
     CDS             complement(165..1718)
                     /locus_tag="VFC06_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase family 1 protein"
                     /protein_id="HZL81186.1"
                     /translation="MTRRTATRRPLAAGAALALTAMIGITAVAMPAAQAATGSANGFA
                     ATNGGTTGGAGGQTVRATTGTAIHTALCTRASSSTPIIIEVEGTINHANTAKVSGNSC
                     NTAAGVIELKQISNVTIVGVGSGAVFDQLGIHIRESSNIIIQNVTVKNVKKSGSPTSN
                     GGDAIGMESDVHNVWVDHATLEASGGESAGYDALFDMKATTKYVTLSYSILRNSGRGG
                     LVGSSDTDLQNGPVTFHHNLYENIDSRTPLLRGATAHIYNNYYAGLNESGINARAGGK
                     AKVENNYFEDSKDVLGTFYTDLPGSWQVAGNIFDNVTWSAKGTDTNPAGPNPTSTTTV
                     SIPYAYTMDGASCVPAIVRQTAGANTGLKVSDGSCTPTTPTPTPTTPAPTTPAPTPTA
                     TTPTGTNLSVGAGADGSSKASGTSYGNVLDGSMSTYWSPSSATGKVSVKWASATTVSM
                     INIREASGAVGNIGAWRVLNGDTGAVLKSGSGAGGISFTATSLKKVTFEITSSTGTPR
                     IGEFETYAK"
     gene            2256..3191
                     /locus_tag="VFC06_04500"
     CDS             2256..3191
                     /locus_tag="VFC06_04500"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HZL81187.1"
                     /translation="MLYAFPDLDLEDSAVLEEIAAMRVELAEHLRVPRRWTGRLRRTA
                     LARAIRGSNSIEGYHVELDDADAALDGEEPLSADQRTFVEIRGYRQAWGYVLAMATDE
                     HFRLDASALRSMHFMMLSHDLSKSPGTYRVRDVYVHDERSEQIVYVGLDPAMVADLLD
                     ELISDLAGHGHEEPAVQAAMAHLNLVMIHPFRDGNGRMARALQTLVLATNGIAEPELA
                     SIEEWLGANTDDYYRALAATGHGVWAPDGDAHLWVKLNLRAHHLQAQTTRRRWQSAQT
                     SYVLLDEVVSAHGLPERVVDPLYTALLGFRLRRPT"
     gene            3363..>4991
                     /locus_tag="VFC06_04505"
     CDS             3363..>4991
                     /locus_tag="VFC06_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007272138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HZL81188.1"
                     /translation="MSGRRSRYREIVETITRYGWGFVLGAVGPGLRSPFGSGGADGSR
                     HMTQPERVRAMVEELGPTFVKLGQILSTRADLLPPAYVAELAKLQDAATPVPFDAVRE
                     TIRDELGQGPEQAFSAFDDLPLASASIGQAHAAEIDHVEVVVKVRRPGAVESVTQDLE
                     ILRELAEIASRTWAPAREYDVVGLVEEFSETMRAELDYVKEARHAERFAEQLAGDPRI
                     HIPRVFWERTTSRVLTLERVRGIKVNDVAALDAAGIDRKDLAVRSATLLCTMIFEDGF
                     FHADPHPGNLFVEADGRIGLIDFGMVGVIDDGLREKLVRALVAVTRKDADRLTRALLD
                     ICGQTHPRDRDALRSSLDGLIRRYAARPLAEIPTAELIAGVIAILRTHHLRLPREMAL
                     LLKVLVMVDGLGRQLDAEFDLWGVLTPFIRRLVVESFAPDAVARRLKAILTDALQFGA
                     DAPDYLRRALAILEQGGFDVHVRTEELRPLLTQVERIGNRLVAGTIAAALIGGVGRVV
                     AADSRQYRSWEEPLVRTGFAALGALGAYLVGSSLR"
BASE COUNT          667 a         1714 c         1772 g          838 t
ORIGIN      
        1 acaaatagca gcacttctac gctacagatc gcagcgccct gacgctccgt gcagcggggc
       61 cagacgcgac ggtctcgcga cgagtcccgg tcgtggggtg gagtcgtgcg gcggctcagc
      121 gccgggatcc gcacgactcc accccgcgtc ggtaacgagg cctgctactt cgcgtaggtc
      181 tcgaactcgc cgatccgcgg cgtgccggtc gagctcgtga tctcgaaggt gaccttcttc
      241 agcgaggtcg cggtgaacga gatgccaccg gcaccgctgc cggacttcag gaccgcaccg
      301 gtgtcgccgt tgaggacccg ccacgcgccg atgttgccga cggcgcccga ggcctcacgg
      361 atgttgatca tcgacaccgt cgtggccgac gcccacttga ccgagacctt gccggtcgcg
      421 ctgctcggcg accagtacgt gctcatcgaa ccgtcgagca cgttgccgta gctcgtcccg
      481 ctggccttgc tcgagccgtc ggcgccggcg ccgacgctga ggttggtgcc ggtcggcgtc
      541 gtggccgtcg gcgtgggagc cggcgtcgtg ggagccggcg tcgtgggcgt cggggtcggg
      601 gtcgtcggcg tgcacgagcc gtccgagacc ttgaggcccg tgttcgcgcc cgccgtctgg
      661 cggacgatcg cgggcacgca gctcgcgccg tccatcgtgt aggcgtacgg gatgctcacc
      721 gtggtggtgg acgtcgggtt cgggccggcc gggttggtgt cggtgccctt ggcggaccag
      781 gtcacgttgt cgaagatgtt cccggcgacc tgccagctgc ccggcaggtc ggtgtagaac
      841 gtgccgagca cgtccttcga gtcttcgaag tagttgttct cgaccttggc cttgccgccg
      901 gcgcgagcgt tgatgccgga ctcgttgagg cccgcgtagt agttgttgta gatgtgcgcg
      961 gtggcgccgc gcagcagcgg ggtgcgcgag tcgatgttct cgtacaggtt gtggtggaac
     1021 gtgacggggc cgttctggag gtcggtgtcg ctggacccga cgaggccacc acgcccggag
     1081 ttgcgcagga tgctgtacga cagcgtcacg tacttggtgg tcgccttcat gtcgaacagg
     1141 gcgtcgtagc ccgccgactc tccgccggac gcctcgaggg tggcgtggtc gacccagacg
     1201 ttgtggacgt cggactccat gccgatcgcg tcgccaccgt tggacgtcgg cgagcccgac
     1261 ttcttgacgt tcttgacggt cacgttctgg atgatgatgt tgctggactc gcggatgtgg
     1321 atgccgagct ggtcgaagac cgcaccgctc ccgaccccga cgatcgtgac gttgctgatc
     1381 tgcttcagct cgatcacgcc ggccgcggtg ttgcagctgt tgccggacac cttggctgtg
     1441 ttggcgtggt tgatggtgcc ctcgacctcg atgatgatcg gggtgctgct gcttgcgcgg
     1501 gtgcacaggg ccgtgtggat cgcggtccct gtggtggcgc gcaccgtctg cccgcctgcg
     1561 ccgccggtcg tcccgccgtt ggtggccgcg aacccgttcg cgctccccgt cgccgcctgc
     1621 gcagcgggca tcgctacggc cgtgatgccg atcatggccg tgagggccag ggcggccccg
     1681 gctgccaggg gtcgtcgtgt cgctgttcgt ctcgtcattg ccactccttc gtcgttgagc
     1741 tcgggcattg ctggcccgcg tcgcgctcgg ggtctcggtc ggtgtctccc atgaaggggc
     1801 tggccgagcc tggcgtgagc gaggtccgcg ctccggcggg cgaccgggat gccggccgtg
     1861 ccgcgtcgtt gcggacttct cctctggacc tcccctgggg tgacggtggc gcggacgcca
     1921 tcgcaccgct cgtcgcgggc caccgccgga cgcccctctg gtgctcgacg cacagctcgc
     1981 gccgagcacc gcacgggtgg attgcggttt cccgaccata ggagaagggt gttcccatga
     2041 gcaagccctc acggttcgcg gctcggggtc caccgctcgg tcatcgcgcg ggatgcccca
     2101 gctgctcgac tggctcgacg ggaccggcga ccgggaccga ggggctgtgc ggcggagtgg
     2161 gaagcacgag tccgcttgcg cgaatgtccc tattcttgcg ccactcgatg ggtgtcgcgg
     2221 cgtgtggcgc gtgaatcgag acattgggac atcccgtgct ctacgccttc cccgacctgg
     2281 acctcgagga ctccgccgtt ctcgaggaga tcgcggccat gcgtgtcgag ctggcggagc
     2341 acctgcgcgt gccgcgccgg tggacgggtc gcttgcgtcg taccgccttg gcccgggcga
     2401 tccgcgggtc gaacagcatc gagggctatc acgtcgagct cgacgacgct gatgcggcgc
     2461 tcgacggcga ggagccgctc agcgccgacc agcgcacctt cgtcgagatc cgtggctacc
     2521 ggcaggcgtg gggctacgtg ctcgccatgg ccactgacga gcacttccgg ctggacgcgt
     2581 ccgcgctacg cagcatgcat ttcatgatgc tgtcccacga cctgtcgaag agcccgggaa
     2641 cgtaccgcgt gcgcgacgtc tacgtccatg acgagcgctc cgagcagatc gtctacgtcg
     2701 gcctcgaccc cgcgatggtc gccgacctcc tcgatgagct gatctccgac ctggccggcc
     2761 acggccacga agagcccgcg gtgcaggcgg cgatggcgca cctcaatctc gtgatgatcc
     2821 acccgttccg ggacggcaac gggcgcatgg cccgggccct tcagaccctc gtgctggcga
     2881 ccaatggcat cgcggagccg gagctcgcga gcatcgagga gtggctcggc gccaacaccg
     2941 acgactacta ccgggccctc gcggcgacag ggcacggggt gtgggcgccc gatggtgacg
     3001 cgcacctgtg ggtcaagctc aacctgcggg cccatcacct gcaggcgcag acgacgcgca
     3061 ggcgctggca gagcgcgcag acgtcatacg tgctgctcga cgaggtcgtg tcggcgcacg
     3121 gcctgcccga gcgggttgtc gatccgctct acaccgcgtt gctcgggttt cgcctgcgcc
     3181 ggcccaccta ggtgcgtggg tcagtagccc gtggctcaaa cgggcccttc ttgccgccgg
     3241 cgccaaaccc ttcggcaggc gattcgactc cggtccggcg ttgccgctac tgacccacgc
     3301 acctagcctg ctgacatggg gacctgctcg cgcggggcag ggcatgccgg gatgagggcg
     3361 ccatgagcgg gcggcggagc aggtaccggg agatcgtcga gaccatcacc cggtacgggt
     3421 ggggtttcgt cctcggcgcg gtcggccccg ggctgcgatc cccgttcggg agcgggggag
     3481 cggacggttc ccggcacatg acgcagcccg agcgtgtgcg cgccatggtg gaagagctgg
     3541 ggcccacgtt cgtcaagctc gggcagatcc tctcgacccg cgcggacctc ctgccgcccg
     3601 cgtacgtcgc ggagctggcc aagctgcagg acgccgcgac acccgttcct ttcgacgcgg
     3661 tgcgcgagac gatccgggac gagctgggcc agggtcccga gcaggccttc tcggcgttcg
     3721 acgacctgcc gctcgcaagt gcctccatcg ggcaggcgca cgcggccgag atcgaccacg
     3781 tcgaggtcgt cgtcaaggtc cgtcgtccgg gcgcggtcga gagcgtgacg caggacctgg
     3841 agatcctgcg ggagctcgcc gagatcgcca gccggacctg ggcgccggcg cgcgagtacg
     3901 acgtcgtcgg gctcgtcgag gagttcagcg agaccatgcg ggccgagctc gactacgtca
     3961 aggaggcgcg gcacgccgaa cgcttcgccg aacagctcgc gggcgatccg aggatccaca
     4021 tcccccgggt cttctgggag cggacgacct ccagggtgct gaccctcgag cgcgtgcgcg
     4081 gcatcaaggt caacgacgtc gccgcgctgg acgcggccgg catcgaccgc aaggacctcg
     4141 cggtgcgctc cgccaccctc ctgtgcacga tgatcttcga ggacggcttc ttccatgccg
     4201 acccccaccc ggggaacctg ttcgtcgagg ccgacggcag gatcggactg atcgacttcg
     4261 gcatggtcgg tgtcatcgac gacgggctgc gcgagaagct ggtccgcgcg ctggtcgccg
     4321 tcacgcgcaa ggacgccgac cgactgactc gagcgctcct cgacatctgc gggcagaccc
     4381 acccccgtga ccgcgacgcg ctgcgctcct ccctcgacgg gctcatccgg cgctatgcgg
     4441 ccaggccgct cgccgagatc ccgaccgcgg agctgatcgc cggggtcatc gccatcctgc
     4501 gcacccacca cctccggctg ccgcgggaga tggccctact gctcaaggtg ctcgtcatgg
     4561 tcgacgggct cggccggcag ctcgatgccg agttcgacct ctggggcgtc ctgaccccct
     4621 tcatccgccg gctggtcgtc gagagcttcg ccccggacgc cgtcgcccgc cggctgaagg
     4681 cgatcctgac cgacgcgctc cagttcggcg ccgacgcccc cgactacctg cgacgggcgc
     4741 tcgccatcct cgagcagggc ggtttcgacg tgcacgtccg gaccgaggag ctccggcccc
     4801 tgctcacgca ggtcgaacgg atcggcaacc gactggtggc cggcacgatc gccgctgcgc
     4861 tgatcggcgg ggtgggccgg gtcgtcgccg cggactcgcg gcagtaccgc tcgtgggagg
     4921 agccgctcgt gcgcaccggg ttcgcggccc tcggcgcgct cggcgcctac ctcgtcggct
     4981 cgagcctgcg a
//
LOCUS       DASRKH010000277         2925 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_58349, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000277 DASRKH010000000
VERSION     DASRKH010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58349"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..1057)
                     /locus_tag="VFC06_04510"
     CDS             complement(41..1057)
                     /locus_tag="VFC06_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family protein"
                     /protein_id="HZL81189.1"
                     /translation="MINYRYLGNSGLKITELTFGNWLTHGSQVENAAAAACVKAALDV
                     GISSFDTADVYANTKAETVLGDILKGERRESLEIFTKVYWPTGPRGANDSGLSRKHIM
                     ESINGSLNRLQTDYVDLYQAHRFDTETPLEETMQAFADVVRQGKAHYIGVSEWTADQI
                     LAGAALAKELGFQLISSQPQYSMLWRVIEDEVVPASKEAGMSQIVWSPVAQGILTGKY
                     KPGQAPPAGSRATDEKGGKNMIGRFFENQELLERVQRLQPVADELDLSMAQLALAWVL
                     QNDNVAAAIIGASRPEQVLENAKASGVEIPAELMARIDDALGDVVQRDPALTTAGAPA
                     GRPS"
     gene            complement(1143..1712)
                     /locus_tag="VFC06_04515"
     CDS             complement(1143..1712)
                     /locus_tag="VFC06_04515"
                     /inference="COORDINATES: protein motif:HMM:NF022686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3043 domain-containing protein"
                     /protein_id="HZL81190.1"
                     /translation="MARKDHHEELAAEAAAAAGKGHATPKRKEREAAQRRALIADPKS
                     GAKERRLRVKEQRAKEQQAMMSGDERHMPAEHKGPVRRYIRDFVDARTSLGEFALPGS
                     IVFVIASLFFNTNPTVGSLIILGFYALVLAAIGDTLWAMRTMKKRVTEKFGAENLGRG
                     WRLYSAARLMNLRRLRVPKPVVRRGEYPV"
     gene            complement(1753..>2925)
                     /gene="nadA"
                     /locus_tag="VFC06_04520"
     CDS             complement(1753..>2925)
                     /gene="nadA"
                     /locus_tag="VFC06_04520"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HZL81191.1"
                     /translation="PALLLLGQRTDPMSERGVDCPGDLPDPSDPDLVERARKAKETLG
                     SRVFILGHHYQRDEVIQFADVTGDSFKLARDAANRPEAEFIVFCGVHFMAESADILTS
                     DKQAVILPDMAAGCSMADMAGISQVEDAWAQLAEAGVSERTIPLTYMNSTAAIKAFCG
                     RHGGAVCTSSNAEVAMRWAFDHVGGVDGEGKVLFMPDQHLGRNTAVLQLGLGLDDCIV
                     WDPRKPLGGNTPEQLEKARVILWKGHCSVHGRFRKDNVDVLRANVPNINILVHPECTH
                     EVVLSADYVGSTEFIINALDAAEPGSAWGIGTELNLVRRLAAAHPDKDVHFLEDTVCF
                     CSTMNRIDLPHLVWALESLVAGGVPNRIVVDDTTQQQARVALNRMLALPAAQPEKD"
BASE COUNT          452 a          925 c          990 g          558 t
ORIGIN      
        1 taggttccgg ggccttcttg cctctctggt gagaatgcgg tcaggagggg cggccagctg
       61 gggcccccgc agtggtgagg gccgggtcgc gctgaacgac gtcgcccagc gcatcgtcga
      121 tgcgggccat gagctccgcc ggaatctcca cgccggaggc cttcgcgttc tcgagaacct
      181 gctcgggtcg cgaggcgccg atgatggcgg ccgccacgtt gtcgttctgc aggacccacg
      241 ccaaggcgag ctgcgccatg ctcagatcga gctcgtcggc aaccggctgg agcctctgca
      301 cgcgctccag gagttcctgg ttctcgaaga agcggccgat catgttcttg ccacccttct
      361 cgtccgttgc gcgcgaaccg gcgggcggcg cctgacccgg cttgtacttg ccggtgagga
      421 tgccttgtgc gacaggagac cacacgatct gactcatgcc cgcttccttg gacgcgggca
      481 ccacctcgtc ctcgatgacg cgccacagca tcgaatactg gggctgcgag gagatcagct
      541 ggaagccaag ttccttggcc agagctgctc ccgcgaggat ctggtcggcg gtccactcgc
      601 tgacgccgat gtagtgcgcc ttgccttgac ggaccacgtc ggcaaaagcc tgcatcgtct
      661 cctcaagggg agtttcggtg tcgaagcggt gcgcctgata gaggtcgacg tagtcggtct
      721 ggagccggtt cagggagccg ttgatggact ccatgatgtg cttgcgactc aggccagagt
      781 cgttcgcgcc ccgtggcccc gtgggccagt agaccttggt gaagatctcg aggctctcgc
      841 gacgttcgcc cttgagaatg tcgccgagga cggtctcggc cttggtgttg gcgtagacat
      901 cggcggtgtc gaaggaggag atgccgacgt caagagcggc cttgacgcac gccgcagcgg
      961 ctgcgttctc gacctgcgaa ccgtgcgtga gccagttgcc aaaggtgagt tcggtgatct
     1021 tgagtccgga gttgcccaga tagcgatagt tgatcatgtg cccacactat ccccgctgag
     1081 cgccccggtc gtaacgtttc gaatccggga cccctccagc atcggatggc tctactggac
     1141 gactagaccg ggtactcgcc gcggcgcacg actggcttgg gcacgcgcag tcggcgcagg
     1201 ttcatgaggc gggccgcgga gtacaggcgc catccgcggc cgaggttttc ggcgccgaac
     1261 ttctccgtga cgcgcttctt catggtgcgc atcgcccaca gggtgtcgcc gatggcggcc
     1321 aacaccaatg cgtagaaccc gaggatgatg aggctgccca ccgtggggtt cgtgttgaag
     1381 aacagcgacg cgatgacgaa cacgatcgac cccggaagtg cgaactcgcc caggctggtt
     1441 cgggcatcga caaagtcacg gatgtagcgg cgtactggcc ccttgtgctc ggccggcatg
     1501 tggcgttcgt cgccgctcat catggcctgc tgttccttcg cgcgctgctc cttcacgcgc
     1561 agtcgacgct ccttcgcgcc cgacttgggg tcagcgatga gcgcgcgtcg ctgcgcagcc
     1621 tcccgctctt tgcgcttggg agtggcgtgg cccttgcctg ctgctgcggc tgcctcggcg
     1681 gcgagctcct cgtggtggtc cttgcgtgcc atgtcgtcct ctcgttagcc gggccagtgg
     1741 tggcggcggc tgtcagtcct tctcgggctg cgcggcgggt agcgcgagca tgcggttgag
     1801 cgcgactcgc gcctgctgtt gcgtagtgtc atccacgacg atgcggttgg ggacgcctcc
     1861 cgccaccaac gattcgagcg cccacaccag gtgcggcaga tcgatgcggt tcatcgtcga
     1921 gcagaagcag acggtgtcct caagaaagtg cacgtccttg tctgggtgtg cggccgccag
     1981 gcggcgcacc aggttcaact cggtgccgat tccccatgcg ctgccgggtt cggccgcatc
     2041 gagggcattg atgatgaatt cggtggagcc cacgtagtcg gcgctcagga ccacctcgtg
     2101 agtgcattcg gggtgtacca gaatgttgat gttcggcacg ttcgcccgca gcacgtccac
     2161 gttgtccttg cggaatcggc catgcacgga gcagtggccc ttccacagga tcacgcgggc
     2221 tttctcgagc tgttcggggg tgttgccgcc cagcggcttg cgggggtccc acacgatgca
     2281 gtcgtccagg ccgaggccaa gctgcagtac ggcggtattg cggcccaagt gctggtcggg
     2341 catgaacagc accttgccct cgccgtcgac cccgcccaca tgatcgaagg cccagcgcat
     2401 cgccacctcc gcgttggagg acgtgcatac ggcgccgccg tgacgcccgc agaacgcctt
     2461 gatggccgcg gtcgagttca tgtatgtcag ggggatggtg cgctcggaga cgcccgcctc
     2521 tgcgagttga gcccacgcgt cctcgacctg gctgatgccg gccatatcgg ccatggagca
     2581 gccggcagcc atgtcgggca ggatcacggc ctgcttgtcg gacgtgagga tgtcggcgct
     2641 ctccgccatg aagtgcacgc cgcagaacac gatgaattcg gcctcggggc ggtttgcagc
     2701 gtcgcgtgcc aacttgaacg agtcgcccgt cacgtcggcg aactggatga cctcgtcgcg
     2761 ctggtagtgg tggcccagaa tgaacacccg gctgccgagc gtctccttgg ctttgcgcgc
     2821 gcgctccacc aaatcggggt cggaggggtc cgggaggtcg cccggacaat cgaccccgcg
     2881 ctccgacatc gggtcggtgc gttgtcccaa taggagaagt gcagg
//
LOCUS       DASRKH010000278         2105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_3809, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000278 DASRKH010000000
VERSION     DASRKH010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3809"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VFC06_04525"
     CDS             complement(<1..619)
                     /locus_tag="VFC06_04525"
                     /inference="COORDINATES: protein motif:HMM:NF042587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HZL81192.1"
                     /translation="MIALTPALQRSDWGTPEGIPQLLGFSADGGPYAEAWWGAHPLVP
                     SGTEEGPLDAVIARDPVGALGAEVADNFGRLPYLLKVLSIARPLSIQVHPTASIAREG
                     FAAEEAQGIAYDDRARTFKDASPKPELLVAMTPMEVLVGLRPESDLRRDLALLDSAGA
                     QALIEALDGGGLAAYVTAALDGAHLDAVTALASLPEGTEGSLGAAR"
     gene            661..1779
                     /gene="serC"
                     /locus_tag="VFC06_04530"
     CDS             661..1779
                     /gene="serC"
                     /locus_tag="VFC06_04530"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020096863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HZL81193.1"
                     /translation="MSTNLAIPDHLLPQDGRFGSGPSKVRAAQVQAIVDANPGVLGTS
                     HRQAPVRGLVRDVRRMLAELYRLPDGYEVLLSNGGSTFVWDAAAFGLISAKAQLAAFG
                     EFGAKFAASVAAAPHLAEPTVHRAVPGSIALPVLEDGVDTYAWPHNETSTGATAPVRR
                     VAGADDALIVIDGTSAAGAIEVDPTQVDFYYFAPQKSFASDGGLWFALTSPAAVERIE
                     RIAASGRYIPPSLSLADAVTNSRAEQTLNTPAISTLIMMRAQLEWMLENGGLAAMAAR
                     SRASSDAIYEWAEARDFASPFVADPAHRSPVVATIDLVDDVDSDEVQAVLRAHGMVDV
                     FPYRKLGRNQLRIGVFPAVETADVEALLACIDWVVEHR"
     gene            1776..>2105
                     /locus_tag="VFC06_04535"
     CDS             1776..>2105
                     /locus_tag="VFC06_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81194.1"
                     /translation="MTPPTPDTAHTSLRRRWAPAFVLVVLLALAGAAIARAAIPPTDD
                     VVAEVQAPAGRVLIPELAAPAPTASPEPFVSPIEVVLPPPPPPPPRSQAATGAAVTTT
                     AAWCDGKY"
BASE COUNT          327 a          749 c          681 g          348 t
ORIGIN      
        1 gacgagctgc gccaagagag ccttccgtgc cctccggcaa cgatgccagc gcggtcaccg
       61 cgtcgaggtg cgctccgtcg agcgcggccg tgacgtatgc ggccagcccg cctccgtcaa
      121 gcgcttcgat gagcgcctgc gcgcccgcac tatcgagtag ggccaagtcg cggcgcaagt
      181 ctgactcggg gcgaagtccc acgagcacct ccatgggcgt catggcgacc aggagttctg
      241 gcttgggcga ggcgtccttg aaggtccgcg cgcggtcgtc gtaggcgatg ccctgcgcct
      301 cttcggcggc aaagccctcg cgagcaatgg aagcggtcgg gtggacctgg atcgacaacg
      361 gccgtgcaat tgacagcacc ttcagcaggt acggcagtcg accaaagttg tcagcaactt
      421 cggcgccgag cgcccccacg ggatcgcggg cgatcaccgc gtccagagga ccctcctcgg
      481 tgcccgacgg caccagaggg tgcgcgcccc accacgcctc cgcgtaaggc ccgccatcgg
      541 cggaaaatcc gagcagttga gggataccct ctggcgttcc ccagtcagag cgctgcaatg
      601 cgggcgtcag tgcgatcacg cattccatca tgtcatcgac tcccctacta ctctgtgcat
      661 atgagcacga acttggcgat cccggaccac ctgctgcccc aagatggccg gttcggatcc
      721 ggcccctcga aggtgcgcgc cgcgcaagtg caggccattg tcgatgcgaa cccgggcgtg
      781 ctgggtacct cgcatcggca ggctcccgtg cgcggtcttg tccgcgacgt caggcggatg
      841 ctcgccgagt tgtaccgctt gcccgacggc tacgaggtgt tgctgtccaa cggtggctcg
      901 actttcgtgt gggacgcggc cgccttcgga ctgatttccg cgaaggccca gctcgccgca
      961 ttcggcgagt tcggtgccaa gttcgccgcg tccgtggccg cggcccctca cttggcggag
     1021 ccgacggtgc accgcgccgt ccccgggtcg atcgcgctac ccgtgctcga ggacggcgtc
     1081 gacacgtacg cctggccgca caacgagacc tcgaccggcg cgaccgctcc cgtgcgccgc
     1141 gtcgccggag cggacgacgc tctcatcgtg atcgacggaa cctcagcagc gggagcgatc
     1201 gaggtggatc ccacgcaggt ggacttctac tacttcgccc ctcagaagtc cttcgcgtcc
     1261 gacggcggcc tgtggttcgc cctcacctca ccagccgccg tggagcgcat cgagcggatc
     1321 gcggcgagcg gccggtacat tccgccgtcg ctgtcgctcg ccgatgctgt caccaattcg
     1381 cgtgccgagc agaccctcaa cacgcccgcc atctccacgt tgatcatgat gcgcgcgcaa
     1441 ctcgaatgga tgctcgagaa tggcggactt gccgcgatgg ccgcgcggtc aagagcgtcg
     1501 tcagacgcca tctacgaatg ggccgaggcc cgagacttcg cgtcgccgtt cgtggctgac
     1561 cccgcgcatc gctcccccgt ggtcgcgacg attgacctgg tcgacgatgt cgactcggac
     1621 gaggtgcagg cagtgcttcg cgctcacggc atggtcgacg tcttccccta ccgcaagctc
     1681 gggcgcaatc aactcaggat tggcgtcttc cccgcggtgg agacggccga cgtcgaggcg
     1741 ctgctcgcgt gcattgactg ggtggtcgaa catcgatgac accgccgacg ccggacaccg
     1801 cccacacttc gctgaggcgt cgctgggccc cggcgttcgt tctggtggtc ctgctggcgc
     1861 ttgccggggc agcgatcgct cgcgccgcca ttccccccac agatgatgtg gtggccgagg
     1921 tccaagcacc cgcgggccgg gttctgatac cggagcttgc ggcgcccgca ccgacggcaa
     1981 gccccgagcc cttcgtgtcg cccatcgagg tggtgctgcc tccaccccca cctccacctc
     2041 cacgcagcca ggcggcgaca ggcgcggcag tcaccaccac cgctgcctgg tgcgatggga
     2101 agtac
//
LOCUS       DASRKH010000279         2097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_52706, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000279 DASRKH010000000
VERSION     DASRKH010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52706"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1232)
                     /locus_tag="VFC06_04540"
     CDS             complement(<1..1232)
                     /locus_tag="VFC06_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HZL81195.1"
                     /translation="MLDVNEFSDLRIGLATAEEIRGWSHGEVKKPETINYRTLKPEKD
                     GLFCEKIFGPTRDWECSCGKYKRVRYRGIVCERCGVEVTRSKVRRERMGHIELAAPVT
                     HIWYFKGVPSRLGYLLDLAPKDLEKVIYFAAYMVTAVDADGRHEDLPQLRNELEQEKK
                     QLVNSRDFGINERAEKLEKDIAELEAEGAKADAKRKVKDGAEREMANIRKRFDAEIDR
                     LDAVFDRFVGLKVQDLEGDELLYRELSRRYGNYFKGSMGAQAIQQRLKDFDLATEADS
                     LRDTIANGKGQRKTRALKRLKVVNAFLTTTNSPVGMVLDAVPVIPPDLRPMVQLDGGR
                     FATSDLNDLYRRVINRNNRLKRLLDLGAPDIIVNNEKRMLQEAVDALFDNGRRGRPVT
                     GPGNRPLKSISDMLKGKQG"
     gene            complement(1247..>2097)
                     /locus_tag="VFC06_04545"
     CDS             complement(1247..>2097)
                     /locus_tag="VFC06_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HZL81196.1"
                     /translation="VISTILPQEDMPFLADGTPVDVILNPLGVPGRMNVGQVLETHLG
                     WVAHEGWDASGVKEDWVKAVPAGSITAAPGTPVATPVFDGISEQTISGLRSVSTPNRD
                     GERLLDETGKTRLYDGRSGEPFPEPIAVGYKYILKLHHLVDDKIHARSTGPYSMITQQ
                     PLGGKAQFGGQRFGEMEVWALEAYGAAYALQELLTIKSDDIIGRVKVYEAIVKGQNIP
                     EPGLPESFRVLMKEMQSLCLNVEVLNAEGQLVEMRESEDDAYQAAESLGISLSSRPDA
                     SSVDEI"
BASE COUNT          366 a          664 c          657 g          410 t
ORIGIN      
        1 ccctgcttgc ccttgagcat gtccgagatg gacttgaggg gacggtttcc gggacccgtg
       61 acggggcgac cgcgacggcc gttgtcgaac agcgcgtcca cagcctcctg caacatgcgc
      121 ttctcgttgt tcacgatgat gtcgggagca ccgagatcaa gcagacgctt caggcggttg
      181 ttgcggttga tgacacggcg gtacaggtca ttgaggtcgg aggtcgcgaa gcggccaccg
      241 tccaactgga ccatcggacg caggtccgga gggatgaccg ggacggcgtc caggaccatg
      301 cccaccgggc tgttcgtcgt cgtcaggaac gcgttgacca ccttgaggcg cttgagcgcg
      361 cgcgtcttgc gctggccctt gccgttggcg atcgtgtcgc gcaagctgtc ggcttcggta
      421 gcgaggtcga agtccttcaa gcgctgctgg atcgcctgag cgcccatcga gcccttgaag
      481 tagttgccgt agcggcgcga gagttcgcgg tacagcagct cgtcgccctc gaggtcctgg
      541 accttgaggc ccacgaaacg gtcgaacacc gcgtcgagac ggtcgatttc ggcgtcaaag
      601 cgcttgcgga tgttcgccat ctcgcgctcg gcgccgtcct tgaccttgcg cttggcatcg
      661 gccttggcgc cctcagcctc gagctcggca atgtccttct cgagcttctc ggcacgctcg
      721 ttgatgccga agtcgcgcga gttgaccaac tgcttcttct cttgctccaa ctcgttgcgg
      781 agctgaggca ggtcctcgtg acgaccatcg gcatccaccg ctgtgaccat gtaggccgcg
      841 aagtagatga ccttctccag gtccttcggg gccaggtcga gcaggtagcc aagacgcgac
      901 ggcacaccct tgaagtacca gatgtgcgtc acgggagcgg cgagttcgat gtgacccatg
      961 cgctcgcggc gcaccttgga gcgggtcact tcaacgccac aacgctcaca gacgatgccg
     1021 cggtaacgga cacgcttgta cttgccgcag ctgcattccc agtcgcgggt cgggccaaag
     1081 atcttctcgc agaacaagcc atccttctcg ggcttgagcg tgcggtagtt gatcgtctcg
     1141 ggcttcttca cttcaccgtg tgaccagccg cggatttctt ccgcagtggc cagtccgatg
     1201 cgcaggtcgc taaattcatt cacgtcgagc actagtgcga ctccccttaa atctcgtcga
     1261 cgctggaggc gtcgggacgg ctggacagag agataccgag cgattcggcg gcctggtaag
     1321 cgtcgtcttc ggactcacgc atctcgacca attggccttc agcgttgagc acctcgacgt
     1381 tcaggcacag cgactgcatc tccttcatga gaacacggaa cgactctgga agccctggct
     1441 ccggaatgtt ctggcccttc acgatggcct cgtagacctt cacacgaccg atgatgtcgt
     1501 cagacttgat ggtgaggagc tcctgcaaag cgtaggcagc gccgtacgcc tcgagggccc
     1561 acacttccat ctcaccgaag cgctggccac cgaactgcgc cttaccaccg agcggctgct
     1621 gggtgatcat cgagtacggg ccagtggagc gggcgtggat cttgtcgtcc accaagtggt
     1681 ggagcttcag gatgtacttg tagcccacag cgatcggctc ggggaacggc tcgccggaac
     1741 gcccgtcgta caggcgcgtc tttcccgtct cgtcgagcaa acgctcgcca tcacggttcg
     1801 gcgtgctcac ggagcgcagg ccgctaatgg tctgctcgga gataccgtcg aagaccggcg
     1861 tggccacggg cgtgccgggc gcggccgtga tcgaccccgc cggaacggcc ttgacccagt
     1921 cttccttgac gcctgacgcg tcccaaccct cgtgagcaac ccacccaagg tgggtctcga
     1981 ggacctggcc tacgttcata cggccgggaa caccgagcgg gttcaggatg acgtctaccg
     2041 gggtgccgtc cgcaaggaac ggcatgtcct cctgcggcag gatcgtcgag atgacgc
//
LOCUS       DASRKH010000280         2373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_9946, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000280 DASRKH010000000
VERSION     DASRKH010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9946"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..1569
                     /locus_tag="VFC06_04550"
     CDS             79..1569
                     /locus_tag="VFC06_04550"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HZL81197.1"
                     /translation="MTARTKDERPTTQARLQSVINLSRNTMRKDAGLNGELDRLPQLA
                     WLLFLKAFDDLEEERAIVERDYRPVLEEQYRWRDWASGKDLTGEPLLRFVNTELLPTL
                     RGLQGTGKAGDPRDTLARVFQDTANKMLSGHLLRQLVDQLDKITFSSSDDMHTMAVFY
                     ESMLREMRDAAGDSGEFYTPRPLIRFIVEQVDPRPGEKVLDPAVGTGGFLVEAYRHMT
                     SGAVSVEDVAVVKRSLRGIEKKPMPYLLAQMNMLLHGIDRPNLVEDNSLIYSLADVKR
                     DGVDVVLTNPPFGGEEEKLVQANFPVNFRTAETAWLFLQSVMARIERSRTGRCGIVVP
                     NSVLFDTGVGGKIKADLMSRFNLHTVVRLPNGVFAPYTIIPSNVLFFEKGKQGPVWYY
                     EISTPDGRKNYTKTKPMRYEEFAECQAWWGGRTREGRVGNEHAWVVPAAEIAASGFNL
                     DLHNPHRPDDLEHRSPRELVAALIETERELLALYERLQQEFEEFRL"
     gene            1581..>2373
                     /locus_tag="VFC06_04555"
     CDS             1581..>2373
                     /locus_tag="VFC06_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81198.1"
                     /translation="MPMGSLGSLVRRPVAIDLEATYRQVGVRGFGRGIFDYEPQLGRD
                     LGKLRFFEVEPKRLVVSNIKAWEGAVAATGENESGRIASNRFLTYEVHGALLEYMAQW
                     FLSDRGLRVLGQASPGSADRNRTLSIAKFEATKVPMPSSTDQERIAAHLDRLMAKIGG
                     WQSPSQQLAFREWDGEPVPVSSLVEMIDRSETVQPDLDYAMQGVRWYGEGLFTREVRA
                     GRELSARTVHRIEPGDLVYNRLFAWKQSFAIATEPGWASNEFPTFR"
BASE COUNT          443 a          800 c          749 g          381 t
ORIGIN      
        1 cggcctcacc gtccacgacc acaacgaggc ctggcgcatc gccctgacca cctacacgaa
       61 cgctctcgag gagacactgt gaccgcgcgc accaaggacg aacgcccgac gacgcaggcg
      121 cgcctgcagt ccgtcatcaa cctgtcccgc aacactatgc gaaaggacgc cggcctcaac
      181 ggggaactcg accggctgcc acaactcgcg tggctcctgt tcctcaaggc cttcgacgat
      241 ctcgaggagg agcgcgccat cgtcgagcgc gactaccgcc cggtcctcga ggaacagtac
      301 cgctggcgcg actgggcctc cggcaaggac ctgaccggcg aacccctgct ccggttcgtg
      361 aacaccgagc tgctccccac cctgcgcggc ctgcagggca ccggcaaggc gggtgacccc
      421 cgggacaccc tcgcccgagt cttccaggac acggcgaaca agatgctctc cggccacctg
      481 ttgcgtcagc tcgtggacca gctcgacaag atcacattct cgtcgagcga cgacatgcac
      541 acgatggcgg tgttctacga gtcgatgctg cgcgagatgc gtgacgccgc cggcgattcc
      601 ggcgagttct acacgccccg gccgctcatc cggttcatcg tcgagcaggt ggacccccgg
      661 ccgggcgaga aggtgctcga cccggccgtg ggcactggtg gcttcctcgt tgaggcgtac
      721 aggcacatga cctcgggcgc ggtctccgtg gaggacgtcg ccgtggtgaa gcgcagcctt
      781 cgggggatcg agaagaagcc catgccctac ctgctggccc agatgaacat gctgctccac
      841 ggcatcgacc ggccgaacct ggtcgaggac aactcgctga tctactccct cgccgacgtg
      901 aagcgcgacg gcgtcgacgt ggtgctcacc aatcctccct tcggcggcga ggaggagaag
      961 ctcgtccagg cgaacttccc ggtcaacttc cggaccgccg aaaccgcgtg gctgttcctc
     1021 cagtccgtga tggcacgcat cgagcgcagc cggaccggcc gctgcggcat cgtggtgccg
     1081 aactcggtgc tcttcgacac cggggtgggc ggcaagatca aggccgacct catgagccgc
     1141 ttcaacctcc acacggtcgt gcggctgccc aacggcgtat tcgccccgta cacgatcatc
     1201 ccgagcaacg tcctgttctt cgagaagggc aagcagggac cggtctggta ctacgagatc
     1261 tccacccccg acgggcgcaa gaactacaca aagaccaagc ccatgcgcta cgaagagttc
     1321 gccgagtgcc aagcttggtg gggtggccgg acccgcgagg ggcgtgttgg gaacgagcac
     1381 gcttgggtgg tccccgccgc ggagatcgcc gcctccggat tcaacctcga cctgcacaac
     1441 ccgcaccgcc ccgacgacct agagcaccgc tccccgcggg aactggtggc cgcgctgatc
     1501 gagaccgagc gggagctgtt ggcgctctac gagcggctgc agcaggagtt cgaggagttc
     1561 cggctgtgaa ggtcgactgg gtgccgatgg ggtctctcgg cagcctcgtg aggcgccccg
     1621 tcgcgattga ccttgaggcg acttaccgcc aagtcggcgt gcgcggattt ggcagaggaa
     1681 tcttcgacta cgagccccaa ctgggcaggg accttggcaa gctccgattc tttgaggtgg
     1741 agcccaaacg tctcgtggtg agcaacatca aggcttggga gggagcggtc gccgcaacgg
     1801 gagagaacga gtccggtcgc atcgcctcca atcgattcct cacctacgag gttcacggtg
     1861 cactccttga atacatggct cagtggttcc tcagcgatag gggcctgcgc gtcttagggc
     1921 aggcgtcacc gggttcagcc gaccggaaca gaacgctttc gatcgccaaa ttcgaggcaa
     1981 ccaaggtgcc tatgccaagc tccaccgatc aggaacgaat cgccgcccat ctggatcgcc
     2041 tgatggccaa gatcggcggc tggcagtccc catcccagca gctcgcattc cgtgagtggg
     2101 acggcgagcc agttcccgtc agctctctcg tcgaaatgat cgatcggtcg gaaacggtgc
     2161 aacccgacct cgactacgcg atgcaggggg tgcggtggta cggcgagggc ctcttcacac
     2221 gggaggtccg ggcgggacgg gagctttcgg cccggacggt ccaccgaatc gagcccggcg
     2281 acctcgtcta caaccggctc ttcgcgtgga agcagtcttt cgccatcgcc accgaaccgg
     2341 gttgggcctc gaacgaattc ccgacattcc gag
//
LOCUS       DASRKH010000281         2389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_6702, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000281 DASRKH010000000
VERSION     DASRKH010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6702"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..1485)
                     /locus_tag="VFC06_04560"
     CDS             complement(109..1485)
                     /locus_tag="VFC06_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002855226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4921 family protein"
                     /protein_id="HZL81199.1"
                     /translation="MTIPAGFAERPARPDYCGAVELAGVEPIRRMADGTIKQVNPMSG
                     TQVWTVPGRGNRPLGNAAGSSAPINPSRLGRYCAFCSGRYWDTPPEKSRLVRTSAGWT
                     RVDNLPANQLTATTADFRRIPNLFEILSLDYWEDNYGYTMPPEARARMDAYLADRDGH
                     AHVMGVARGKAMASGVPERVWDQMSEEKRLEWADNFFAGGHDVIVARRHFADGATCED
                     HLASSGTLTPEEHFQYTAYTIDTMRDLYDFAPHVKYVATFQNWLRPAGASFNHLHKQL
                     VAIDERGVQAEREYERAKADPNIYNEMAVDYAAAQGLLLAENTHAIAFAGFGHRYPTL
                     EVYSKANARMPWKHTPDQVRAVSDLLHACHAATGATVATNEEWYYKPRDLDVPMPWRI
                     MVKWRVSTLAGFEGGTKINVNTISPFTLHDRVLPRLLELRKQGLIAPMRLGSECQPEK
                     GCLRYLSR"
     gene            1490..1792
                     /locus_tag="VFC06_04565"
     CDS             1490..1792
                     /locus_tag="VFC06_04565"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZL81200.1"
                     /translation="MDTAAGSIEITRNDSLSRYEIRQDGEFVGLADFFDDDGVRAFPH
                     TEVDPAQRGHGLVGKLVGWALRDTRESGLKADPVCPYVATFIRRHPEYTDLLVQDA"
     gene            1904..2386
                     /locus_tag="VFC06_04570"
     CDS             1904..2386
                     /locus_tag="VFC06_04570"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HZL81201.1"
                     /translation="MGTMTVTLHTAITDDAIDTLFREARTVVAFSDQPVDRAVVGEIF
                     ESFKYGPTMMNNFHETLSTTFPHAPNAGKMFQDREQRGYLARMNASLQMGYFIIAARA
                     HGLDVGPMTGYNGPAIAERFLSDGDQDLLAVVALGYADESAGRPRSPRLPFEQVVEFI
                     "
BASE COUNT          349 a          822 c          818 g          400 t
ORIGIN      
        1 gatgattccg cgcgggtcgt tgaaccagtt ggggccttgc atggcggcct gggtcagcgt
       61 ggctggcgtt cacggtgtcg gaccgggggg agcggcgccg cttcgaggtc acctgctgag
      121 gtaccgcagg cagcccttct ccggctgaca ctccgagccg agccgcatcg gcgcgatgag
      181 cccttgcttc cgcagttcga ggagccgcgg cagcacacgg tcgtggaggg tgaagggcga
      241 gatcgtgttg acgttgatct tggtgccgcc ctcgaaaccc gcgagggtgg agacgcgcca
      301 cttcaccatg atccgccagg gcatcgggac gtccaggtcg cgcggcttgt agtaccactc
      361 ctcgttcgtg gccacggtgg cgccagtggc ggcgtggcag gcgtggagga ggtccgagac
      421 cgctcggacc tggtccgggg tgtgcttcca gggcatgcgg gcgttcgcct tcgagtacac
      481 ctcgagggtg gggtagcggt ggccgaaccc ggcaaacgca atggcgtgcg tgttctcggc
      541 caggagaagc ccctgggcgg cggcgtagtc cacggccatc tcgttgtaga tgttcgggtc
      601 cgcctttgcc ctctcgtact cgcgctcggc ctggaccccg cgctcgtcga tcgcgaccag
      661 ctgcttgtgc aggtggttga acgaggcgcc ggcggggcgc agccagttct ggaaggtggc
      721 cacgtacttc acgtgcgggg cgaagtcgta caggtcccgc atggtgtcga tagtgtatgc
      781 ggtgtactgg aagtgctcct cgggcgtcag ggttccggag gaggccagat ggtcctcgca
      841 ggtggcgccg tcggcgaagt ggcgccgggc cacgatcacg tcatgaccgc cggcaaagaa
      901 gttgtcggcc cattcgagcc gcttctcctc gctcatctgg tcccacacac gctccgggac
      961 gcccgaggcc atcgccttgc cccgagccac ccccatcacg tgggcgtggc catcgcggtc
     1021 cgcgaggtag gcgtccatcc gggcgcgcgc ctcggggggc atcgtgtacc cgtagttgtc
     1081 ctcccagtag tccagcgaaa ggatctcgaa gaggttcggg atgcggcgga agtccgcggt
     1141 ggtggcggtc agctggttgg ccgggaggtt gtccacccgg gtccagcccg cgctggtccg
     1201 aaccagacgg gacttctcgg gtggggtgtc ccagtagcgg cccgagcaga aggcgcagta
     1261 gcggccaagg cgactcgggt tgatgggcgc cgatgacccg gcggcgttgc cgagcgggcg
     1321 gttcccgcgc cccgggacgg tccacacctg ggtgccggac atgggattga cctgtttgat
     1381 ggtgccgtcg gccatccggc ggatcggctc cacgcctgca agttccacgg ctccacaata
     1441 gtccgggcgc gcggggcgtt cagcgaaccc ggcgggtatc gtcatcggca tggacacggc
     1501 cgccggctcg atcgagatca cccgcaacga ctccctttcg cgctacgaga tccgccagga
     1561 cggcgagttc gtcggcctgg ccgacttctt cgacgacgac ggcgtgcgcg ccttccccca
     1621 caccgaggtc gaccccgccc agcgcgggca cggcctcgtc ggcaagctcg tgggttgggc
     1681 gctgcgcgac acccgcgagt caggcctcaa ggcggacccc gtgtgtccct acgtcgccac
     1741 gttcatccgc cgccaccccg agtacaccga cctgctggtc caggacgcct gacgccgtcg
     1801 ccgtcgccgc cgtcgcccgc ccgacgccgt ccccgcccga cgccgtcgcc cgtgaccacc
     1861 gccacggcgc ccccgctggc gatttggttg aaggttcaat aacatgggaa ccatgacagt
     1921 caccctgcac accgccatca ccgacgacgc gatcgacacc ctcttccgtg aggcgcgcac
     1981 ggtcgtggcc ttctctgacc aaccggtgga ccgcgctgtg gtcggggaga tcttcgagtc
     2041 gttcaagtac ggccccacca tgatgaacaa cttccacgag acgctttcca ccacgttccc
     2101 gcacgccccg aacgccggga agatgttcca ggaccgcgag cagcgcggct acctcgcccg
     2161 catgaacgcc agcctgcaga tgggctactt catcatcgcc gcccgcgccc acgggctcga
     2221 tgtcggcccg atgaccggct acaacggccc ggccatcgcg gagcgcttcc tcagcgacgg
     2281 ggatcaggac ctgctcgccg tcgtcgccct cggctacgcg gacgagtccg ccggccgtcc
     2341 gcgctccccg cgcctgccgt tcgagcaggt tgtcgagttc atctaggcg
//
LOCUS       DASRKH010000282         2118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_62563, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000282 DASRKH010000000
VERSION     DASRKH010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62563"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..>2118
                     /locus_tag="VFC06_04575"
     CDS             175..>2118
                     /locus_tag="VFC06_04575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HZL81202.1"
                     /translation="MRWLPSWRVVLGTFIALAAVATGLLIAAYASIDVPAPDDFAQAE
                     TTEVYFADGTTLMGSFSEYDRRSVDFATLPPHVGQAVVASEDRRFYQNNGVDLQGVAR
                     ALWNNLRGLPTQGGSTITQQYVERYYMGTTTSYVGKFKEAILAVKIDRQQAKDEILGS
                     YLNTIYFGRGAYGIEVAAQNYFGKPAAELTLAESALLAGIIPSPSNYDPAVDPVTAEQ
                     RWNRVLDYMAEDGWITAEERAAQVFPATIDYVPADTFAGTNGYLLDMVRTELADKAGI
                     SQAETDTAGLTIVTTIEKPRQDAAVAAVDALPDDRPDNNRVGLVSMDPATGRIVALYG
                     GPDFLTQPRNAVTQDRAQGGSTFKPFTLIAALEGGASLQDQYESYSPMEIEGYDVPVN
                     NYDSRNRGRIDILRATQDSVNTVFVQLNVEYGPAQMVDVATRMGMPADTPGLIDSPSN
                     VLGPASPHAIDTLTAYGTIASGGTKHEPYLVDSVQDSDGNTRFKGASEGEKVIDSDVI
                     ANATYAMQAVVEGGSGKTAREIGRPAAGKTGSSNAYRSAWFAGFVPQLVTVVNMYQVG
                     PNGEEEVLTGFGGVRTIAGGTWPTRVWTDYMKVALDGVEVQDFPEPVIKPPTHTPSPS
                     PSPSPTPTPTPTPTPTPTPTPEPT"
BASE COUNT          347 a          775 c          721 g          275 t
ORIGIN      
        1 acggcgtccg gtgcgccgtc gtcggccgca cccgcggcct cgcggcgcaa agggggggga
       61 gcgcgcggcg gctccggctc cggcggctcc ggcggcggct cgggctccgg cggctcgggc
      121 gggagttccg gggcaaaggg ccggcggtac ccgcggcccg gcaagggtcc ggtcgtgcgc
      181 tggctgccga gctggcgtgt ggtgctgggc acgttcatcg cgctggcggc ggtcgcgacc
      241 ggactcctca tcgctgccta cgcgtcgatc gacgtccctg cgcccgatga cttcgcgcag
      301 gcggagacca ccgaggtcta cttcgcggac gggacgaccc tcatgggctc gttctccgag
      361 tacgatcgcc ggtccgtcga cttcgccaca cttcccccgc acgtgggcca ggccgtggtg
      421 gcctccgagg accgccggtt ctaccagaac aacggcgtgg acctgcaggg cgtcgcacgc
      481 gcgctgtgga acaacctccg gggcctgccc acccagggtg gctcgacgat cacgcagcag
      541 tacgtggagc gctactacat gggcaccacc acctcctacg tgggcaagtt caaggaggcg
      601 atcctcgcgg tcaagatcga ccggcagcag gccaaggacg agatcctcgg gagctacctg
      661 aacacgatct acttcgggcg gggcgcgtac ggcatcgagg tggcggcgca gaactacttc
      721 gggaagccgg ccgccgagct gaccctcgcg gagtccgcac tcctcgcggg gatcatcccc
      781 tcgccgtcga actacgaccc cgcggtggac ccggtcacgg ccgagcagcg ctggaaccgc
      841 gtgctggact acatggcgga ggacggctgg atcaccgcgg aggagcgcgc cgcccaggtg
      901 ttccccgcca cgatcgacta cgtcccggcc gacaccttcg cgggcaccaa cggctacctg
      961 ctggacatgg tgcgcaccga actggcggac aaggcgggca tttcccaggc cgagacggac
     1021 accgccggcc tcaccatcgt caccacgatc gagaagccgc ggcaggacgc cgcggtcgcg
     1081 gcagtggacg cgctgcccga cgaccgcccg gacaacaacc gcgtgggcct cgtctcgatg
     1141 gacccggcaa ccggccggat cgtggcccta tacggcggcc ccgacttcct gacccagccg
     1201 cgcaacgccg tcacccagga ccgcgcccag ggcggttcca cgttcaagcc gttcacgttg
     1261 atcgcggcgc tcgagggcgg cgcgtccctg caggaccagt acgagtcgta cagcccgatg
     1321 gagatcgagg gctacgacgt tccggtgaac aactacgact cgcgcaacag gggccgcatc
     1381 gacatcttgc gggccaccca ggactcggtg aacaccgtct tcgtgcagct caacgtggag
     1441 tacggacccg cgcagatggt ggacgtggca accaggatgg gcatgcccgc ggacaccccc
     1501 ggcctgatcg actcgccgtc gaacgtgctc ggcccggcca gcccgcacgc catcgacacc
     1561 ctcacggcgt acggcacgat cgccagcggg ggcacgaagc acgagcccta cctcgtcgac
     1621 tccgtgcagg actcggacgg caacacccgc ttcaagggcg cttccgaggg cgagaaggtg
     1681 atcgactcgg acgtgatcgc gaacgccacc tacgccatgc aggccgtggt ggagggcggc
     1741 tccggcaaga ccgcccgcga gatcggccgc ccagcggccg gtaagaccgg ctcctccaac
     1801 gcctaccgct ccgcgtggtt cgcgggcttc gtgccgcaac tcgtgaccgt ggtgaacatg
     1861 taccaggtgg gtcccaacgg cgaggaggag gtgctcaccg ggttcggtgg ggtccgcacc
     1921 atcgccggcg gcacctggcc cacccgggtg tggacggact acatgaaggt ggccctcgac
     1981 ggcgtcgagg tgcaggactt cccggagccc gtcatcaagc caccgacaca caccccgtcg
     2041 ccgtcgccgt cgccgtcgcc cacgccgacg cccacgccga cgcccacgcc gacgcccacg
     2101 ccgacgcccg agccgacg
//
LOCUS       DASRKH010000283         2310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_62931, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000283 DASRKH010000000
VERSION     DASRKH010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62931"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VFC06_04580"
     CDS             complement(<1..184)
                     /locus_tag="VFC06_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81203.1"
                     /translation="MRTTYGEHPCLPVQIHTMAVETGLLERLAQDPLEPVRLLALSAL
                     QYARDAAEAKAAARRAP"
     gene            complement(181..>2310)
                     /locus_tag="VFC06_04585"
     CDS             complement(181..>2310)
                     /locus_tag="VFC06_04585"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HZL81204.1"
                     /translation="TSPPGDDMHSTRAPWPTAAAPSIALPPPERSWPSSALAVAAATL
                     MLAGGGHVIGRGPHPAVVVASGVAVLALVVAHGVRQSRRGQALDMVVLAIHPMLGWRV
                     PTRSSATGARWSRGRAPVPARLRISYTPGAADTVDWSAKVAEIVSRRLGVAYSVALFD
                     QRRCRLQLHGVPDNDTATRSPAVARAERVVRSIFGEGASVELDVVDDELTAVRVRHAL
                     GAKVARQHARSFIENSIGALLPGRWRATWELTEDRVRFEQRPTMPTYVPHVPEPITKV
                     NLKRITLAIDEYNDPICWNLVGTGPHLIVVGKTGTGKTVAINGVVMEAARRGWPVWIC
                     DPKRIEFMGLRDWPNVQIVATTVADMIATIYAAHELMERRFAVIEDGADEGDFEPLVV
                     VLDEFRNFHRQVAAWYTAVKLRGMAAKCPVFDWVAAIAEKGRAASIHLVLGTQRPDAD
                     FMTGSMRDNFDSRLALGRLSPQGAQMMWESPYVGVSVPRSIRGRGTGITDTDRVTEVQ
                     VLWTPDPRRAARDNKTADLALLDGLRPAATSHHRLEVDLGAEADLEGNEISEWHRVLE
                     SRLVPAGEAVTRPLSGAAAALRQRSGLRTLMSGRAAATPFDETDQGSIGESADYDDEA
                     APDLLEGYGPAITTAASRLAVGDLVVVDELVDQWAAVEAVDVDPTGDEYVCVSWRADD
                     GEVGDLSLQDDDAVTVRRPLAEPEETS"
BASE COUNT          328 a          813 c          834 g          335 t
ORIGIN      
        1 ccggcgcacg ccgggccgcg gccttggcct cggccgcgtc gcgcgcgtac tgcagagcac
       61 taagcgccag gagccggacg ggctcgagcg ggtcctgcgc cagacgctcg aggagcccgg
      121 tctcgactgc catcgtgtgg atctggacgg gcaggcacgg gtgctcgccg taggtggtcc
      181 tcacgaggtc tcctcaggct cagcgagagg gcgtcggacg gtgactgcgt cgtcgtcctg
      241 caggctcaga tcaccgacct cgccgtcgtc ggcacgccag gacacgcaca cgtactcgtc
      301 cccggtcggg tcgacatcga cagcctctac ggcggcccac tggtcgacga gctcgtcgac
      361 gaccacgagg tcgccgacgg ccaggcggct ggctgcggtt gtgatggccg ggccgtatcc
      421 ctccagcagg tccggggcgg cttcgtcgtc gtagtcggcc gactcgccga tcgagccctg
      481 gtcggtctcg tcgaacggtg tcgccgcagc gcgcccggac atgagcgtgc gcaggccgct
      541 gcgctggcgc agtgccgcgg ccgcgccgct gaggggccgc gtgacggctt cgcctgcggg
      601 cacgagcctc gactcgagca cccggtgcca ttcgctgatc tcgttgccct caaggtcggc
      661 ttcggcaccg aggtcgactt cgaggcggtg gtggctggtc gcggcggggc gcaggccgtc
      721 cagcagcgcg aggtcggcgg tcttgttgtc gcgggccgcg cggcgcggat cgggggtcca
      781 gagcacctgg acctcggtga cccgatcggt atcagtgatc ccggtgccgc ggccgcggat
      841 gctgcgcggg accgacacgc ccacgtacgg cgactcccac atcatctggg cgccctgcgg
      901 cgacagtcgg ccgagcgcga gccgggagtc gaagttgtcc cgcatgctgc cggtcatgaa
      961 gtcggcgtct gggcgctgag tgcccagcac caggtggatg ctggcagcgc gacccttctc
     1021 cgcgatcgcc gcgacccaat cgaacaccgg acacttggcg gccatgccgc gcagcttcac
     1081 cgccgtgtac caggcggcca cctggcggtg gaagttgcgg aactcgtcga gcaccaccac
     1141 gagcggctcg aagtcaccct cgtcggcgcc atcctcgatc acggcgaaac gccgctccat
     1201 cagctcgtgc gccgcgtaga tcgtggcgat catgtcagcc accgtggtgg cgacgatctg
     1261 cacgttcggc cagtcgcgca ggcccatgaa ctcgatccgc ttggggtcgc agatccagac
     1321 aggccagccg cggcgggcgg cttccatcac aacaccgttg atcgccacgg tcttgccggt
     1381 cccggtcttg cccacgacga tgagatgcgg accggtcccc accaggttcc agcagatcgg
     1441 gtcgttgtac tcgtcgatcg ccagggtgat ccgtttgagg ttcaccttgg tgatcggctc
     1501 ggggacgtgc ggcacgtacg tcggcatggt cggtcgctgc tcgaaccgga cgcggtcctc
     1561 ggtcagctcc cacgtggcac gccaccgccc tggcagcagg gccccgatcg agttctcgat
     1621 gaagctacgt gcgtgctgcc gcgccacctt cgcgccgagg gcgtgccgca cccgcactgc
     1681 ggtgagctcg tcgtcgacga cgtcgagctc gacgctcgcg ccctccccga agatgctccg
     1741 caccacgcgc tcggcgcgag cgactgccgg cgacctggtg gccgtgtcgt tgtccggcac
     1801 cccgtgcagc tgcaggcggc agcggcgctg atcgaaaagc gcgaccgagt aggccacgcc
     1861 caggcgtcgg gagacgatct cggccacctt ggcagaccag tccacggtgt ccgcggcgcc
     1921 cggtgtgtag gagatccgca gccgggcggg caccggggca cggccacggc tccagcgcgc
     1981 cccggtggcg ctggagcggg tcggcacgcg ccagccgagc atcggatgga tcgccaggac
     2041 gaccatgtcg agcgcctgcc cgcggcgcga ctgacgcacc ccgtgcgcga cgacgagagc
     2101 caggaccgcc accccggacg ccacgaccac cgcggggtgg gggccgcggc caatgacgtg
     2161 gcccccaccg gccagcatga gcgtggccgc agcgacggcg agggccgacg aaggccacga
     2221 acgttccggt gggggcagtg cgatggacgg cgcagcagcg gtgggccacg gggcgcgggt
     2281 cgagtgcatg tcgtctcccg gaggtgaggt
//
LOCUS       DASRKH010000284         2637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_18273, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000284 DASRKH010000000
VERSION     DASRKH010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18273"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="VFC06_04590"
     CDS             complement(<1..896)
                     /locus_tag="VFC06_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZL81205.1"
                     /translation="MMNDEPQLLAGRYEVGDLIGRGGMAEVHIGYDTRLGRNVAIKIL
                     RADLARDPSFQTRFRREAQAAAGLNHPSIVAVYDTGEDHRRNENGAVQAVPFIVMEYV
                     EGHTVRDILKGDVAAPIEEAVEITQGVLAGLDYAHHAGLVHRDIKPANVMLTPTGAVK
                     VMDFGIARALADIGQTMTQTQAVVGTAQYLSPEQARGENVDARSDLYSTGCLLFELLT
                     GRPPFMGDSPVSVAYQHVREEAPRPSQFASDVPPELDAIVGKALQKDRNARYSSALEF
                     SAIHGALQVGGELQSRRVPRVA"
     gene            complement(952..1914)
                     /locus_tag="VFC06_04595"
     CDS             complement(952..1914)
                     /locus_tag="VFC06_04595"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZL81206.1"
                     /translation="MQSGTTLGGRYVLRSLLAVGGMGEVWRGIDSELDRPVAIKVLKE
                     EAQSNETFLKRFRNEAKNAAGLMHPNIAQVFDYGDQAGTAYLVMELVEGEPLSTVLER
                     ELTVDEHRLVRMMIGTCQGLHAAHQAQVMHRDIKPGNLLIEGEDHVKITDFGVSRSHD
                     QTALTATGMVMGTAQYLAPELALGKPATPASDLYALGIIAYESVVGRRPFTASSPVDI
                     AIAQVNEPVPPLPSSVSPALAALIMDLLEKNPKRRPSSALELEGILTQLRLPTKAERV
                     ARERGAPETIPPKRTGNAPEHAMPPSIAPARYRPRPDAPSIRHT"
     gene            complement(1924..>2637)
                     /locus_tag="VFC06_04600"
     CDS             complement(1924..>2637)
                     /locus_tag="VFC06_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HZL81207.1"
                     /translation="TATIKNYGGERCGASDNIPLIDALRVSCNTAFADLAMRLGWGVI
                     ERTAAKFGWEQSLNIPLSVTPSRLPDSPNNPQIAQSGIGQFDVRTTPLQMAMVASAIA
                     NGGVLMKPYLVSTVRDPDLKVIEHTSPEVFSSSLSPDTAGALNAMMQAVVSDGTGSNA
                     QIAGITVAGKTGTSETGTDTAPHTWFIAFAPANDPVVAVAVLVENGGDLGNEATGGRV
                     SAPIAKAVIQAAIDRAAVG"
BASE COUNT          388 a          794 c          960 g          495 t
ORIGIN      
        1 atcgcaacgc gcggtactcg tctgctttgg agttctccgc cgacctgcag cgcgccatgg
       61 atggcggaga actccaaagc agacgagtac cgcgcgttgc gatccttctg gagcgccttg
      121 ccgacgatcg cgtcgagttc agggggcacg tcggaggcga actgggaggg ccgcggtgcc
      181 tcttcgcgca cgtgctggta ggcgacggac acgggagagt cgcccatgaa cggcgggcgt
      241 ccggtgagca gctcgaacag gaggcagccg gtcgaataca ggtcggagcg ggcgtcgacg
      301 ttctcaccgc gtgcctgttc gggggacagg tattgggcgg tgccgacgac ggcctgggtc
      361 tgcgtcatcg tctgcccgat gtcggcaagg gcgcgggcga tcccgaagtc catgaccttg
      421 accgcgccgg tgggcgtgag catgacgttg gcgggcttga tgtcgcggtg caccaggccc
      481 gcgtggtggg cgtagtcgag cccggccagc acgccctgcg tgatctcgac ggcctcctcg
      541 atgggggccg cgacgtcgcc cttgaggatg tcgcggaccg tgtggccctc gacgtattcc
      601 atgacgatga aggggacggc ctgaacggcg ccgttctcgt tgcgacggtg gtcctcgccg
      661 gtgtcgtaca cggcaacgat cgacgggtgg ttgaggccgg ccgcggcttg agcctcgcgg
      721 cggaatcgcg tctggaagga ggggtcgcgc gcaaggtccg cgcgtaatat cttgatggcg
      781 acgttgcggc caaggcgggt gtcatagccg atgtggacct cggccattcc gcctcgaccg
      841 atgaggtcgc cgacctcata ccggccagct agtagctgtg gttcgtcgtt catcattttc
      901 cttgcctgta gcccgagggc acgttatctc acgcttattg tggcattccc actacgtgtg
      961 gcgaatcgac ggcgcgtcgg gacgcggtcg atacctcgcg ggagcaatgg acggcggcat
     1021 ggcatgttcg ggtgcgttgc cggttctctt cggtggaata gtctcggggg cgccgcgctc
     1081 gcgagcgact ctttcggcct tggtcggcag tcgcagttgt gtgaggattc cctcgagctc
     1141 gagcgcggaa ctcggccgac gcttggggtt cttctccaac agatccatga tgagcgccgc
     1201 cagcgcgggc gacacgctgg acgggagggg cggcacgggc tcgttgactt gggcgatcgc
     1261 gatgtccacg ggtgacgacg cggtgaaggg ccgtcttccc accacggatt cgtaagcgat
     1321 gattcccagc gcgtacaggt ccgacgcggg agtggcgggt ttgccgagcg cgagttcagg
     1381 agcgaggtat tgggcggtgc ccatcaccat gccggtggcg gtcagcgcgg tttggtcgtg
     1441 gctgcgggag acgccaaagt cggtgatctt gacgtggtcc tcgccctcga tcagcaggtt
     1501 gccgggcttg atgtcgcggt gcatgacctg ggcctggtgc gccgcatgga ggccctggca
     1561 tgtgccgatc atcatgcgca ccagtcggtg ttcgtccacc gtgagctcgc gctcgagcac
     1621 ggtggacagc ggttcgccct ccaccagttc catgacgagg taggcggtgc cggcctggtc
     1681 gccatagtcg aacacctggg cgatgttggg gtgcatcagg cccgcggcgt tcttggcctc
     1741 attgcggaag cgcttgagga acgtctcgtt gctctgcgcc tcttccttga gcaccttgat
     1801 ggcgacgggc cggtcgagtt cgctgtcgat gccgcgccac acctcgccca tgccgcccac
     1861 cgcgagcagg gagcgcagca cgtagcggcc gccgagtgtc gtgcctgact gcattggcat
     1921 gagctacccc accgccgcac ggtcgatagc cgcttggatg acggccttgg cgattggggc
     1981 cgaaaccctg ccgccggtgg cctcgttgcc gaggtctcca ccattctcga ccagaacggc
     2041 cactgccacc accggatcgt tcgcaggggc gaaggcgatg aaccaggtgt ggggcgcggt
     2101 gtcggtgccc gtctcggagg tgccggtctt gcccgccacg gtgatgcctg cgatctgcgc
     2161 attactgccg gtgccgtcgg acacgaccgc ctgcatcatc gcgttgagcg ctccggccgt
     2221 gtcagggctg agagaggagg agaacacctc gggtgacgtg tgctcgatga ccttgaggtc
     2281 cggatcccgc acggtcgaca cgaggtacgg cttcatgagg acgccgccgt tggcgatcgc
     2341 ggaggccacc atcgccatct ggaggggcgt ggtgcgcacg tcgaattggc cgatgccgga
     2401 ttgggcgatc tgggggttgt tcgggctgtc gggcagccgc gacggcgtga cggacagggg
     2461 aatgttcagc gactgttccc agccgaactt ggcggcggtg cgctcgatga cgccccagcc
     2521 caagcgcatg gcgaggtccg cgaaggccgt gttgcacgac acccgaaggg cgtcgatgag
     2581 cgggatgttg tcgctggccc cgcagcgttc gccgccgtag ttcttgatgg tggcggt
//
LOCUS       DASRKH010000285         2238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_37773, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000285 DASRKH010000000
VERSION     DASRKH010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37773"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="VFC06_04605"
     CDS             <1..574
                     /locus_tag="VFC06_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZL81208.1"
                     /translation="LVHGVDLWQHPDVAKPMLGVLPDGLRTFDRLTGGELISYAGVLR
                     GLDPDVVRSRRDDLLVALDLGTATGTLVADFSAGMAKKIGLACALVHAPKVLVLDEPF
                     EAVDPVSGAAIRTILQAFVGSGGTVVMSSHVMALVERLCDRVAIVGAGRILTEGVLDD
                     VRGAGDLEARFVELVGDVSEQRDLWWLQQS"
     gene            634..2157
                     /locus_tag="VFC06_04610"
     CDS             634..2157
                     /locus_tag="VFC06_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81209.1"
                     /translation="MAAGAVWSVSLIPALLFASQVLSTRGLDIKQDALVAVGTLLAFA
                     WVVVPLLATGVDDSLDPARFAPWGIPLRRLMPGIVVASFTTVPAIFFAAVAGVMAATW
                     RGADHGMWVLVVASVGAALSFATWVASARLAALWATRLLATRASKLVLAGLAIAALAL
                     VAATISVVRADGLDSFLGGEVATTLQALARTPWAAGFAAPTAVALGQPWGAVWRLVMV
                     AAWLVTLLAAWRSAVEHSLVNPVSRSGGTLRKSDSILAAVERRIPGWRATPTTRAVLA
                     RSARSWRIDPRYVAQVVGAVSMPTIMGGIAVAALGSGGPWLFAVPVVLSVTIGWGRHN
                     DLAYDASAVWLDIVSGVRGAEVARGRLLATVGWAAPTVVVMCVVAAAITDRWDALPAV
                     GLVALGVLGVGLGIASISSILLPYRVPAPGESPFGAEPGSIGASLGAQVASSLVTGVA
                     VPLVVAPLVAAFVWGGPWWFISAAMGLVSGVAGVWGGTRIAGALYERRQGRLIAMIS"
BASE COUNT          262 a          708 c          836 g          432 t
ORIGIN      
        1 gttggtgcac ggggtagacc tgtggcagca cccggacgtc gccaagccga tgctgggagt
       61 gcttcccgat gggctacgca ccttcgaccg cctcaccggc ggcgaattga tctcctacgc
      121 gggtgtcttg cgcgggcttg accccgacgt cgtgcgctcg cggcgcgacg acctgctggt
      181 cgcactcgac cttggcactg ccacgggcac cctcgtggcc gacttctccg cgggcatggc
      241 caagaagatc ggtctcgcgt gcgcgctggt gcatgcaccc aaggtgctcg tgctcgacga
      301 gccgttcgaa gccgtcgacc ccgtgtcggg cgccgccata cgcacgattc tccaggcgtt
      361 cgtcggatcc gggggaacgg tggtcatgtc gtctcacgtg atggcgctcg tcgagcggtt
      421 gtgtgaccgg gtggccatcg tgggcgcagg tcgcatcctg accgagggag tcttggacga
      481 cgtgcgaggg gcgggggatc ttgaggcgcg gttcgtcgaa ctggtaggag acgtgagcga
      541 gcagagggac ctgtggtggt tgcagcaatc ctgagcctca ggtggcgcat gatgcgtcac
      601 caattccgcc gcgattggtg gaggctgctg ttcgtggctg cgggcgccgt gtggtcggtc
      661 tcgctgatcc ccgcactcct gttcgcgagc caggtgctga gcacgcgcgg cctcgacatc
      721 aagcaggatg cgctggtagc ggtcggaacc ctcctcgcgt tcgcctgggt tgtggtgccc
      781 ttgttggcga ccggcgtgga cgactcactc gacccggcgc gcttcgcgcc gtgggggatt
      841 ccactgcggc ggctgatgcc gggcatcgtc gtcgcctcct tcacgactgt cccagccatc
      901 ttcttcgccg cggtcgcggg ggtgatggct gcgacgtgga ggggagcgga ccacggaatg
      961 tgggtcttgg tcgtcgcgtc cgtgggcgcg gctctctcct tcgccacctg ggtggcgtcc
     1021 gcgcgattgg cggctctctg ggccacccgc ttgctggcga cgcgcgcctc caagctggtg
     1081 ctggccgggc tcgcgattgc cgcgctcgcg ctggtggccg cgacgatctc cgtggtgcga
     1141 gccgatggcc tcgattcctt cctcggcggc gaagtcgcga cgaccttgca ggcgctcgcc
     1201 cgcactccgt gggcggctgg attcgcggcg ccgaccgctg tggcactcgg ccagccgtgg
     1261 ggcgccgtgt ggcggctcgt gatggttgcg gcctggctcg tgacgcttct ggcggcgtgg
     1321 cgctccgcgg tggagcactc gctcgtgaat ccggtatcgc gttccggggg aacgctgcgc
     1381 aagagcgaca gcatccttgc tgcggtggag cgacggattc ctgggtggcg ggcgacgccg
     1441 actacccgtg ctgtgctcgc gcgctccgcc agatcgtgga ggatcgaccc ccggtacgtc
     1501 gcccaggtgg tcggagccgt ctccatgccg acgatcatgg gcggcatcgc cgtggccgcg
     1561 cttggttcag gtgggccgtg gctgttcgcc gtgcctgtgg tgttgagcgt caccatcggt
     1621 tggggccgcc acaacgacct cgcctacgac gcatcggcag tgtggctcga catcgtgtcc
     1681 ggggtgcgtg gcgccgaggt cgcgcgcgga cgcctgcttg cgacggtggg gtgggcagcg
     1741 ccgacggtgg tggtgatgtg cgttgtggct gccgcaatca cggaccgctg ggacgcgcta
     1801 ccggccgtgg ggctcgtggc gttgggcgtg ctgggcgtcg gccttggcat cgcctccatc
     1861 tcatcgatcc tgctgccgta tcgggttcca gcgccaggag agagcccctt cggcgctgag
     1921 cccggcagca ttggcgcgag cctcggcgcc caggtcgcgt cgtcgctcgt caccggtgtg
     1981 gcggtgcccc ttgtcgtggc gccgctggtc gcggcatttg tgtggggcgg gccgtggtgg
     2041 ttcatctcgg cagcgatggg tctggtgagc ggtgtcgcag gcgtgtgggg agggacccgc
     2101 atcgcgggtg cgctgtacga aagacgccaa ggtcgcctga tcgcaatgat ttcttaaggc
     2161 aacttctgtg ccaaccggct tacgatgggg ccatgagcga gaccactgac gcacccacca
     2221 cgggcggctc cctgtcga
//
LOCUS       DASRKH010000286         2160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_58558, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000286 DASRKH010000000
VERSION     DASRKH010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58558"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..1356)
                     /gene="xseA"
                     /locus_tag="VFC06_04615"
     CDS             complement(67..1356)
                     /gene="xseA"
                     /locus_tag="VFC06_04615"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017824593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HZL81210.1"
                     /translation="MADAHTDNDSAPRILPDAPASLAATAADTSADRPWPVRHLSPKI
                     GEYIARMSPVWVEGQVLNVKRWRHLVFLTLRDTDEQLSLKATLPASEVDALGVSLEDG
                     ARVVIHAKPQWWPRGGDLQMVGDQVRSVGLGDLLARIEALKVALAAEGLFSADRKVAL
                     PVIPRRIGLVVATQGDAEHDVVTNAIDRWPAARFEIRRVTVQGPRAVADVAAALQELD
                     SLDEVDVIVVARGGGSLEDLLPFSDEKLVRAAADIVTPLVSAIGHEMDSPLLDLVADY
                     RASTPTDAGKRIVPDAAAETERLQGARALMVAALSIRVRRERERVADLRARPVIAQPV
                     RMLEPHRDKLRHARANLRMATATAVARARGEFASAAASLRALSPQSTLDRGYAVIRDA
                     FGTVITSPADVSVDDELSVRVAAGGLKARVTDVAQPS"
     gene            1387..>2160
                     /locus_tag="VFC06_04620"
     CDS             1387..>2160
                     /locus_tag="VFC06_04620"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HZL81211.1"
                     /translation="MPPSSTKKVLLAAPRGYCAGVDRAVIAVEKALELHGAPIYVRKE
                     IVHNKYVVETLTDRGAIFVSETDEVPEGARVVFSAHGVSPAVRDAAAARQLTTIDATC
                     PLVTKVHKEAVRFARDNQTILLIGHTGHEEVEGTAGEAPENTIVVNSPDDVEGLEVPD
                     PSNVVWLSQTTLSVDETMDTVRLLRAKFPELQDPPSDDICYATQNRQVAVKKIAPQVE
                     LMIVVGSANSSNSVRLVEVALQSGADASYRIDRARELRDE"
BASE COUNT          352 a          714 c          746 g          348 t
ORIGIN      
        1 gtccaagagc ggcgccagga cggggttcct gggcagcttc gcggccttcc gcgcgtaggc
       61 ccggcgtcag cttggctggg cgacgtctgt gacgcgcgcc ttgagaccgc ccgctgcgac
      121 gcgcaccgag agttcgtcgt ccaccgaaac atctgccggc gatgtgatca cagtcccgaa
      181 cgcgtcgcga atgaccgcgt agccccggtc gagggtcgat tgcgggctga gcgcgcgcag
      241 cgacgcggct gctgatgcga actctcctcg cgctcgcgcc acggcagtgg cggttgccat
      301 gcgcaggttt gcccgcgcgt ggcgcagctt gtcgcggtgt ggctcgagca tgcgtactgg
      361 ctgcgcaatc accggtcgtg cgcgcaagtc cgccactcgt tcgcgttctc tgcgcacgcg
      421 aatcgacaaa gccgccacca tgagcgcgcg ggcgccctgg aggcgttccg tctcggccgc
      481 ggcatcgggc acgatgcgct ttcccgcgtc ggtaggcgtg gaggcgcgat agtcggcgac
      541 caagtcgagg aggggactgt ccatttcgtg gccgattgcg ctgaccaacg gcgtgacgat
      601 gtcggccgcg gcgcgcacca gtttctcgtc gctgaacggc aggaggtcct cgagtgagcc
      661 acctccgcgc gcgaccacga tcacgtcgac ctcgtccagt gagtcaagtt cttggagcgc
      721 cgcagccaca tcggcgacgg ccctcggccc ctgcacggtg acgcggcgga tctcgaaccg
      781 ggcggcgggc cagcggtcga tcgcgttggt gacaacgtcg tgctcggcat caccctgggt
      841 ggcgaccacc aggccgatgc ggcgggggat caccggcaag gcgaccttgc ggtcggcgga
      901 gaacaggcct tcggctgcaa gggcgacctt gagcgcctcg atgcgtgcga gcaggtcgcc
      961 caggcccacc gagcgcacct ggtcgccgac catctgcagg tcgcctcccc gcggccacca
     1021 ctgcggcttg gcatggatga ccacgcgcgc accgtcctca agcgagaccc ccaaggcatc
     1081 gacctcggac gccggaaggg ttgccttgag cgacaattgc tcgtcggtat cgcgcagcgt
     1141 gaggaacacg aggtggcgcc aacgcttgac gttgagcacc tgcccctcga cccagacggg
     1201 actcatgcgc gcgatgtatt cgccgatctt ggggctcagg tgccggaccg gccacggcct
     1261 gtccgcgctc gtgtccgctg ccgtcgcagc gagcgacgcg ggggcgtcgg gaaggatgcg
     1321 cggtgcgctg tcgttgtctg tatgagcgtc ggccaccctc ccagcctccc acgtaggatt
     1381 gagtacatgc ctcccagtag caccaagaaa gttctccttg ctgccccgcg cggatactgc
     1441 gccggggtcg acagggccgt cattgccgta gagaaggcgc tcgagttgca cggagcaccc
     1501 atttacgtgc gcaaggagat cgtccacaac aagtacgtgg tcgagaccct gacggaccgt
     1561 ggcgccatct ttgtgtccga gacggacgag gtgcccgagg gtgctcgcgt cgtgttctca
     1621 gcgcacggag tatcgccggc ggtgcgcgac gccgcggcgg cgcgacagct gaccacgatc
     1681 gatgccacct gcccgctcgt gacaaaggtg cacaaggagg ccgtgcgctt cgcgcgcgac
     1741 aaccagacga tcctgctcat cggccacacg ggccacgaag aggtcgaggg gacggcgggc
     1801 gaggccccag agaacacgat cgtcgtgaat tcgcccgacg atgttgaggg ccttgaggtg
     1861 ccggacccga gcaacgtggt gtggttgtcg cagaccaccc tgtccgtgga cgagacgatg
     1921 gacacggtgc gcctcttgcg cgccaagttc cccgagcttc aggacccgcc gagcgacgac
     1981 atttgctatg ccacgcagaa ccgtcaggtc gccgtgaaga agatcgcgcc ccaggtggag
     2041 ctcatgatcg tggtgggctc tgccaactcg tcgaactcgg tgcgactcgt ggaggtggca
     2101 ctccaatcgg gggccgatgc gtcgtatcgc attgaccgcg cgcgcgagtt gcgcgacgaa
//
LOCUS       DASRKH010000287         2440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_35269, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000287 DASRKH010000000
VERSION     DASRKH010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35269"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..>2440
                     /gene="rpoB"
                     /locus_tag="VFC06_04625"
     CDS             2..>2440
                     /gene="rpoB"
                     /locus_tag="VFC06_04625"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015770873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HZL81212.1"
                     /translation="MEAARKAGRNDVPEIAGLQEIFEEISPIEDFGQTMSLSFSDPYF
                     EDPRHTPEECKEKDFTYAAQLFVTAEFMNNTTGEIKSQTVFMGDFPLMSPRGTFIING
                     TERVVVSQLVRSPGVYFEFTADKTSDKDIYTAKMIPSRGAWLEFEIDKRDAVGVRVDR
                     KRKQSVTLFLKALGLSDEDIQREFADYPSVLETLEKDTVHTQDEALVDLYRKLRPGEP
                     PTVEAGQNLLDNFYFNPKRYDLAKVGRFKLNKKLGVGKALNDSVLGVDDIVATVKYIA
                     AAHAGHTTLKGANGEVFVETDDIDHFGNRRIRAVGELIQNQIRTGLSRMERVVRERMT
                     TQDVEAITPQTLINTRPVVAAIREFFGTSQLSQFMDQNNPLAGLTHKRRLSALGPGGL
                     SRDRAGMDVRDVHPSHYGRMCPIETPEGPNIGLIGSLASFGRINPLGFVETPYRKVLK
                     GKITDQVDYLTADDEDRYVIAQANAQVDDKRGFLEERVLVRAKGGEVDFVAPEAVDYI
                     DVSPRQMVSVATALIPFLEHDDANRALMGANMQRQAVPLVRSEAPLVGTGMELRAAVD
                     AGDVVVATSPGVVTEVSSDLVTVANDDGTTGSYRIAKFQRSNQGTSYNQRVIVDEGAR
                     LEIGTVIADGPATDQGDLALGKNVLVAFMCWEGHNFEDAIILSQRLVQDDTLSSIHIE
                     EHEVDARDTKLGPEEITRDIPNASEEVLADLDERGIVRIGAEVRAGDILVGKVTPKGE
                     TELTPEERLLRAIFGEKAREVRDTSLKVPHGEMGTVIGVRVFTEEDGDDLPAGVNELV
                     RVYIAQRRKIQEGDK"
BASE COUNT          462 a          804 c          754 g          420 t
ORIGIN      
        1 tattgaagcc gcacggaagg caggtcgtaa tgacgtgccg gagattgcgg gacttcagga
       61 gatcttcgaa gagatctccc cgattgagga cttcggacaa accatgtccc tgtccttctc
      121 ggacccctac ttcgaggatc cgcgtcacac tccggaggag tgcaaggaga aggacttcac
      181 ctacgcggca caactttttg tcaccgctga gtttatgaac aacacgacgg gtgagatcaa
      241 gtctcagacc gtcttcatgg gcgacttccc actcatgagc ccccgcggca cgttcatcat
      301 caacggcacc gagcgtgtcg tcgtgtccca gctggttcgc tcgcctggcg tgtacttcga
      361 gttcacggcg gacaagacgt ccgataagga catctacacg gcgaagatga tcccgtcgcg
      421 tggtgcctgg ctcgagttcg agatcgacaa gcgcgacgcc gtcggcgtcc gcgtcgaccg
      481 caagcgcaag cagtcggtga ccctgttcct caaggccctc ggcctgtccg acgaggacat
      541 ccagagggaa ttcgccgact acccgtcggt gctcgagacc cttgagaagg acacggttca
      601 cacgcaggac gaggcgctgg tggatctgta ccgcaagctg cgtccgggcg agccgcccac
      661 cgtcgaggcg ggccagaacc tgctcgacaa cttctacttc aaccccaagc gctacgacct
      721 ggcgaaggtg ggccgcttca agctcaacaa gaagctgggc gttggcaagg cgctgaacga
      781 ctcggtgctg ggcgtcgacg acatcgtcgc gaccgtcaag tacatcgctg ccgcgcacgc
      841 cggtcacacg acgctcaagg gcgccaacgg cgaggtcttt gtcgagactg acgacatcga
      901 ccacttcggc aaccgccgca tccgtgcggt gggcgaattg attcagaacc agatccgcac
      961 gggcctgtcc cgcatggagc gcgttgtccg cgagcgcatg acgactcagg acgtcgaggc
     1021 catcacgccg cagaccctga tcaacacgcg tcccgtggtt gccgccattc gcgagttctt
     1081 cggaacctcg cagctgtcgc agttcatgga ccagaacaac ccactcgcgg gtctgaccca
     1141 caagcgtcgc ctgtcggcgc ttggcccggg tggtctgtcc cgtgaccgcg caggcatgga
     1201 cgtgcgcgac gttcacccgt cgcactacgg ccgcatgtgc cccattgaga cccctgaagg
     1261 cccgaacatc ggcctgatcg gttcgctcgc ttccttcggt cgcatcaacc ccctcggctt
     1321 tgtcgagacg ccgtaccgca aggtcctcaa gggcaagatc accgaccagg tcgactacct
     1381 caccgccgac gacgaggacc gctacgttat cgcccaggcc aacgcccagg tggacgacaa
     1441 gcgcggcttc cttgaagagc gcgtcctcgt gcgcgccaag ggtggagagg tcgactttgt
     1501 ggcgccggag gccgtcgact acatcgatgt gtcgcctcgc cagatggtgt cggtcgcgac
     1561 cgcgctgatc ccgttccttg agcacgacga tgccaaccgc gccctcatgg gcgcgaacat
     1621 gcagcgtcag gccgtgccgc tcgtccgttc cgaagcgccg cttgtcggta ccggtatgga
     1681 gctccgtgcg gctgttgacg caggtgacgt ggtcgtggct accagccccg gcgtcgtgac
     1741 cgaggtcagc tccgacctgg tgaccgttgc gaacgacgac ggcaccacgg gctcgtaccg
     1801 catcgccaag ttccagcgtt cgaaccaggg caccagctac aaccagcgcg tcatcgtcga
     1861 cgagggtgcg cgcctcgaga tcggcactgt catcgccgat ggcccggcca ccgatcaggg
     1921 tgacctcgct ctgggcaaga acgtcctcgt ggcgttcatg tgctgggaag gtcacaactt
     1981 cgaggacgcg atcatcctgt cgcagcgcct tgtccaggac gacaccctct cctcgattca
     2041 catcgaggag cacgaggttg acgcccgcga cacgaagctg ggtccggaag agatcacgcg
     2101 cgacatcccc aacgcctccg aagaggtgct ggccgacctc gacgaacgcg gcatcgtccg
     2161 catcggcgcc gaggttcgtg ctggcgacat cctcgtgggc aaggtgacgc ccaagggcga
     2221 gaccgagctc acgcccgagg agcgcctgtt gcgcgccatc tttggtgaga aggcccgcga
     2281 ggtgcgcgac acgtcgctca aggttcctca cggtgagatg ggcaccgtca tcggcgtccg
     2341 cgtgttcacc gaggaggacg gcgacgacct tcctgccggc gtcaacgagt tggtgcgtgt
     2401 ctacatcgct cagcgacgca agatccagga gggcgacaag
//
LOCUS       DASRKH010000288         2475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_25529, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000288 DASRKH010000000
VERSION     DASRKH010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25529"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VFC06_04630"
     CDS             <1..346
                     /locus_tag="VFC06_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81213.1"
                     /translation="AVAVPYTYSVHEALGAAATAGVEEIVVLGADDAPAGRFPIEQAE
                     AVPESARPTTSLQSVTTPVPRGSEIPAGALSDVMIPALREWWGKTDVWVVRDGEAVVG
                     IVRLVDVLAALK"
     gene            365..1348
                     /locus_tag="VFC06_04635"
     CDS             365..1348
                     /locus_tag="VFC06_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HZL81214.1"
                     /translation="MTLPAPVGAAERRGELRVGDRVQLTDPKGRHHTIVLAEGATFHT
                     HRGYFTHDDILGRAEGSVVRNTAGIDYVVLRPLLSDFVMSMPRGAAVVYPKDSAQIVQ
                     YADVYPGARVVEAGVGSGALSMSLLRAVGDQGSLLSIERREDFADIARANVESFFGAP
                     HPAWEIRIGDFDAHARSLDEGSVDRVVLDMLAPWENLEAAAHALAPGGVFLAYVATTT
                     QLSRLAETLKGTGDFTEPRAWETMARTWHLEGLAVRPDHRMVGHTGFLLSSRRMAPGV
                     EAPIRHRRPQGTPAPTDDWSPEDLGERPVSDKKVRKVRRDVTKLLQGQEEE"
     gene            1351..>2475
                     /gene="arc"
                     /locus_tag="VFC06_04640"
     CDS             1351..>2475
                     /gene="arc"
                     /locus_tag="VFC06_04640"
                     /EC_number="5.6.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR03689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HZL81215.1"
                     /translation="MVDPQDAIAALEKRNGELQRLLEVARDQLGEMRTKLAEVSAPPQ
                     TFATFVQWAGKNADIVANGRRMRVAVLPAVAEGLTSGDEVLLNAMSVIVGTAEPERSG
                     QAAIVREVIDDDRVLVVSRGEDERVVILMGGDAAARVHEGDTVIIDPRTGFASQKIER
                     TGVEALLLEEVPDVDYSVIGGLGGQIERIRDAIELPLRYPELYREHALQPPRGMLLYG
                     PPGCGKTLIAKAVAHSLGEATGADRSYFLSVKGPELLNKYVGETERYIRTIFERARAK
                     AHTGVPVVIFFDEMDALFRARGSGVSSDMETTIVPQLLTELDGVEALGNVIVIGASNR
                     EDMIDPAILRPGRLDVKIEISRPDAAASVDILTKYLTDDLP"
BASE COUNT          419 a          786 c          860 g          410 t
ORIGIN      
        1 ggctgttgcg gtgccgtaca cgtactcggt gcacgaggcg ctcggcgccg ccgccaccgc
       61 gggagtggaa gagatcgtcg tcttgggcgc cgacgatgct ccggctggca ggtttccgat
      121 cgagcaggct gaggctgttc cagagtcggc gcgcccgacg acgtccctgc agtcggtcac
      181 gacgccggtt cctcgcggtt ccgagattcc ggcgggcgcc ctatcggacg tcatgatccc
      241 agcgctgcgc gagtggtggg gcaaaacgga tgtctgggtg gtgcgagacg gcgaggccgt
      301 cgtgggaatt gtgaggctag ttgacgtcct cgcggccctg aaataggccc gtagactggc
      361 gcgcatgacc ctcccagccc ctgtgggcgc cgctgaacgg cgcggcgagt tgcgcgtcgg
      421 cgaccgcgtc cagttgaccg atcccaaggg caggcaccac acgatcgtgc tggccgaagg
      481 ggccaccttc cacacgcacc gcggttactt cacacacgac gacattctgg gtcgcgccga
      541 gggaagcgtg gttcgcaaca ctgcgggcat cgactacgtc gtactgcgcc cgctgctgag
      601 cgacttcgtg atgtcgatgc cgcgtggcgc ggccgtcgtg tatcccaaag actccgcgca
      661 gatcgttcaa tacgccgacg tgtaccccgg agcgcgagtc gtcgaggctg gcgtcggctc
      721 cggagcgctg agcatgtcgc tgctgagagc ggtcggggac caaggctcgc tgctgtcgat
      781 cgagcgccgc gaggacttcg ccgacatcgc tcgcgccaac gtcgagtcct tcttcggtgc
      841 gccccacccg gcgtgggaga tccgcatcgg cgactttgat gcgcacgcgc gctcgcttga
      901 cgagggcagc gtcgaccgcg tggtgcttga catgttggcg ccgtgggaga acctcgaggc
      961 ggccgctcac gcgctcgcgc ccggcggcgt cttccttgca tacgtggcga ccaccacaca
     1021 actatcccgc ctggcagaga ccctgaaggg aaccggagac ttcacagagc cgcgcgcttg
     1081 ggagacgatg gcaaggacgt ggcatcttga ggggttggcc gtgcggccag accaccgcat
     1141 ggtggggcac accggtttcc tcctctcgtc acgccgcatg gcgccgggtg tggaggcacc
     1201 catccggcat cggcggcccc aaggaacgcc cgcgccgacg gacgactggt cgcccgagga
     1261 cctcggggag cgcccggtgt cggacaagaa ggtgcgcaag gtgaggcgcg acgtgacgaa
     1321 acttctacaa ggacaggaag aggaatgacg gtggttgacc ctcaggacgc catcgccgcg
     1381 ctggagaagc gcaacggcga attgcagcga ctgctcgagg tggcgcgcga ccaattgggc
     1441 gagatgcgca ccaagctggc cgaagtgtcg gcgcccccgc agacgttcgc cacttttgtg
     1501 cagtgggcag gcaagaatgc cgacatcgtt gccaacggcc gacgcatgcg cgttgccgtc
     1561 ctccccgcgg tggcggaggg tctcacgtcc ggcgatgaag tgcttctcaa tgcgatgagc
     1621 gtgattgtcg ggaccgccga gccggaacga tcaggccagg cggccatcgt gcgcgaggtc
     1681 atcgacgacg accgcgtgct cgtcgtgtcg cgcggcgagg acgagcgcgt cgtgattctc
     1741 atgggcggcg acgctgccgc gcgggtgcac gagggcgaca ccgtgattat cgatccccgg
     1801 accggtttcg cgtctcagaa gatagagcgc acgggagtcg aggcgctgct tctcgaggag
     1861 gttcccgacg tcgactactc ggtgatcggc ggacttggcg gccagattga gcgcattcga
     1921 gacgccattg aactgccgct gaggtatccc gagttgtatc gcgagcacgc gctccagccg
     1981 ccgaggggga tgctgctata cggccctccc ggttgcggca agacgctcat cgcgaaggca
     2041 gtggcgcact cgcttggcga ggcgaccggc gcagaccgca gctacttcct gtcggtcaag
     2101 ggccccgagt tgctcaacaa gtacgtgggc gaaactgaac gctacattcg caccatcttt
     2161 gagagggcgc gcgccaaggc gcacaccggc gtgcccgtcg tgatcttctt cgacgagatg
     2221 gacgcgctgt tccgcgcgcg cggctcgggc gtgagctccg acatggagac caccatcgtt
     2281 ccgcaactgt tgaccgagct ggatggcgtc gaggccctcg gcaatgtcat cgtgattggc
     2341 gcgtccaacc gtgaggacat gatcgatccg gcgatcctgc ggccgggaag gcttgacgtc
     2401 aagattgaga tctcgcgtcc ggacgccgca gcctcggtcg acatcctgac caagtacctg
     2461 acggacgacc tgccc
//
LOCUS       DASRKH010000289         2351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_43025, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000289 DASRKH010000000
VERSION     DASRKH010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43025"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1292
                     /locus_tag="VFC06_04645"
     CDS             <1..1292
                     /locus_tag="VFC06_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HZL81216.1"
                     /translation="TGLILEEAIEPLTDPVHLDGVTVDDDAARAALAKTAIIKLNGGL
                     GTSMGMDRAKSLLQVRDEQSFLDVIVGQVQRARKATGVHLPLVLMNSFRTRDDSLEAL
                     AAHRGVQVEGLPLDFVQNKEPKLLVDGLTPVEWPADPDLEWCPPGHGDIYTAILASGV
                     LDALLEAGFRYASVSNSDNLGAVPNARIAGWFAASGAPYAAELCERTSADRKGGHLAV
                     RKADGQLILRDTAQTADDEMHYFTDEHHHPFFHTNNLWWDLEQLKAALTERGAVLGLP
                     LIRNTKNVDPADRESPQVFQIETAMGAAIEVFEGATAIVVGRDRFLPVKTTNDLLVLR
                     SDAYELQEDGALRLAVRRAPLIDLDPAYYKTMADFDARFPHGAPSLREASSLTVKGDW
                     TFGKNVVVRGTVVLQDDDGQRNAIASGTMLDGVVMEG"
     gene            complement(1442..>2351)
                     /locus_tag="VFC06_04650"
     CDS             complement(1442..>2351)
                     /locus_tag="VFC06_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81217.1"
                     /translation="KRVLLVVGPATYDTVLGTFGTYHFTWVSIAITLAATAAVPLLLM
                     LLFRVVPLLSIDEIEEIEEASTGHTPTEPNRSTGREASPVGATGTSHRVASTIGVLML
                     VGFMGVLGVGAAGPAQAATPQSASVSSSLSSATGEPTITVEGVEGGGKVQLTATLTVA
                     NGQPQADAPVEFSFTTTQFGKPARLVPLGTVTTDTSGVAKLILGGGADRMYLPTTVGP
                     QEFVATFTPTTADAKPISSSTTVNITVARSAYTPAPPKPLASLGKGLVVALFAIVVAV
                     WLTLITQIWRVRRVRLATPEPTTNTA"
BASE COUNT          380 a          753 c          842 g          376 t
ORIGIN      
        1 ccaccggcct catcctcgag gaggcgattg agccgctgac cgaccccgtt cacctggacg
       61 gcgtgacggt ggacgacgac gcggcgcggg ccgcgctcgc caagaccgcc atcatcaagc
      121 tcaacggcgg gctcggcacc tcgatgggga tggaccgcgc caaatcgctg cttcaggtgc
      181 gggacgagca gagcttccta gacgtgattg tggggcaggt gcagcgggcc cgcaaggcga
      241 cgggggtcca cttgccattg gtgctgatga actcgttccg cacgcgcgac gactcgcttg
      301 aggcgctcgc cgcgcaccgc ggcgtgcaag tcgagggcct gccgctcgac tttgtgcaga
      361 acaaggaacc caagctgctg gtggacggcc ttactcccgt cgagtggccc gcagacccgg
      421 acctcgagtg gtgcccaccc ggccacggcg acatctacac ggcgatcctc gcatcgggcg
      481 tgctcgatgc gctgctggag gccggcttcc gctacgcgtc ggtgtccaac tcggacaacc
      541 tgggcgccgt gcccaacgcg cgcatcgccg gctggttcgc cgcatcgggc gcgccgtatg
      601 ccgccgagtt gtgcgagcgc acctccgctg accgcaaggg cggccacctg gccgtgcgca
      661 aggcggacgg ccagctcatc ctgcgcgaca ccgcccagac cgccgacgac gagatgcact
      721 acttcacgga cgagcaccac cacccgttct tccacaccaa caacctgtgg tgggacctgg
      781 agcagttgaa ggcggcactg acggagcgcg gcgccgtgtt gggcctgccg ctcatccgca
      841 acaccaagaa cgtggacccc gcggacaggg aatcacccca ggtgtttcag atcgagaccg
      901 ccatgggcgc agcgatcgag gtgttcgagg gcgccaccgc gatcgtggtg ggccgcgacc
      961 gcttcctccc cgtcaagacc acgaacgacc tgctggtgct gcgctccgac gcctacgagt
     1021 tgcaggagga cggcgccctg cgcctcgcgg tcaggagggc cccgctgatc gacctcgacc
     1081 ccgcgtacta caagaccatg gccgacttcg acgcccgctt cccgcacggc gcaccgtcgc
     1141 tgcgcgaggc atcgtcgctg acagtcaagg gcgactggac gttcggcaag aacgtcgtgg
     1201 tgcgcggcac cgtggtgttg caggacgacg acggccagcg caacgcgatc gccagcggca
     1261 cgatgctcga cggcgttgtg atggagggct aggtgtgcaa ctgacgtagc actgcggcac
     1321 gtgacgcgaa ctcgccttga cggcgcgcgt actcattctt gtcttcggcg gtctcgatcg
     1381 cgatcggcta cggcgagttc gagcactcgg tagaaggtca gctgaatagc cctgaggcgt
     1441 cctatgcggt gttggttgtt ggctctgggg tggcgaggcg gacgcgtcgg acccgccaga
     1501 tctgggtgat gagggtgagc cagacggcta cgacgatggc gaagagggcg acgaccaggc
     1561 ctttgcctag gctggcgagc ggtttgggtg gtgcgggggt gtaggcggat cgggcgacgg
     1621 tgatgttgac ggtggtgctg gagctgatcg gcttggcgtc ggcggtggtg ggggtgaagg
     1681 tggcgacgaa ctcctgcggc ccgaccgtgg tgggcaggta catgcggtcg gcgccgccac
     1741 caaggatcag cttggcgact ccgctggtgt cggtggtgac ggtcccgagc ggcacgagcc
     1801 gggctggctt cccgaactgg gtggtggtga aggagaactc cactggtgcg tcggcttgtg
     1861 gttggccgtt ggcgacagtc agggtcgcgg tgagctgcac cttcccgccg ccctcgacgc
     1921 cctcgacggt gattgtcggc tcgcccgtcg cgctactcag cgacgaactc actgacgcgc
     1981 tttgcggagt cgcggcctgc gcgggtccag ccgcgcctac cccgagcacg cccatgaaac
     2041 caacgagcat caggacgccg atggtgctgg ccactcggtg ggacgtacct gtggcgccaa
     2101 ccggactcgc ctcgcgtcct gtagaccgat tcggttcggt gggcgtgtgg ccggtcgagg
     2161 cctcctcgat ctcctcgatc tcgtcgatgg acagcagcgg gacgacccgg aagagcagca
     2221 tgagcaggag cgggacggcc gcggtcgcgg cgagagtgat cgcgatcgag acccaggtga
     2281 agtggtaggt gccgaaggtg cccagcaccg tgtcgtaggt ggcagggccg acgaccagca
     2341 ggacccgctt g
//
LOCUS       DASRKH010000290         2028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_22837, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000290 DASRKH010000000
VERSION     DASRKH010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22837"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VFC06_04655"
     CDS             <1..189
                     /locus_tag="VFC06_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81218.1"
                     /translation="TDALARFGPAALGPVLAALLALGRLRDEEADAAIRGAVGSDDPR
                     IGALARRLMRDRSQPPPA"
     gene            404..1663
                     /gene="eno"
                     /locus_tag="VFC06_04660"
     CDS             404..1663
                     /gene="eno"
                     /locus_tag="VFC06_04660"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HZL81219.1"
                     /translation="MAENFVVTDLSALEILDSRGNPTVRVEVTLESGASGVAGVPSGA
                     STGSREAVELRDGDKDRYSGKGVTKAVANANGEVADLVKSRSWSSLAEVDQALNEADG
                     TGNKARLGANALVGVSMALARALAAPGELYAYLNSPGVPLRLPVPHFNVLNGGAHAPN
                     SLDFQEFMVAPIGAPSLPEAVRAGAGVYAKLRSLLQAQGFATGLGDEGGFAPDITRPE
                     DVLALLVEAIEGAGYTAGRDGVAIALDPAASEFYKGGAYHVAGEALSTADMIERYAAM
                     VADYPVWSIEDGLAEGDWDGWAAMTAQLGDKVQLVGDDLFVTNPAIITEGIAKKVANS
                     ALIKLNQIGSVSETLEAMRLCREAGYTQMVSHRSGETEDSFIADLAVATGCGQLKTGA
                     PARGERVAKYNRLLQIAATTDLPYGLG"
BASE COUNT          297 a          686 c          750 g          295 t
ORIGIN      
        1 accgatgcgc ttgcgcggtt cggccctgcg gcgctgggtc cggtgctcgc ggccctgctg
       61 gcactcggcc ggctgcgcga cgaggaggcg gacgccgcga tccgtggcgc cgtcggctcc
      121 gacgacccgc gaatcggggc cctggcacgg cgcctcatgc gggaccggag ccagcctcca
      181 cccgcgtgaa ggtggccacc agattggttc ggcgaagtgc ctcgaaccgg aaggcgtggg
      241 ggagtaaggt cgcccacatc acggcggtgg ggctcgcctt caaccgcggc agcaatgccg
      301 acaacagtcc ctgacccttg gcgaggacga gggaggctgt gattcgcgcg gccggacctc
      361 ccctcttggt gacgacgccg cggcgtcgtg gaggagttgg aacatggcag agaacttcgt
      421 tgtcactgac ctgagtgcgc tggagatcct ggactcgcgg ggcaacccca ccgttcgcgt
      481 ggaggtgacg ctcgagtcgg gcgcgagcgg cgtggcgggg gtcccgtcgg gcgcgtccac
      541 ggggagccgg gaggcggtcg agctgcgcga cggcgacaag gaccggtact ccggcaaggg
      601 cgtcacgaag gcggtggcca acgccaacgg cgaggtcgcg gacctggtca aatcccgctc
      661 gtggtcctcg ctcgcggagg tggaccaggc actgaacgag gccgacggca ccgggaacaa
      721 ggcccgcctg ggcgcgaacg ccctcgtggg cgtgtcgatg gcgctggccc gtgcgctcgc
      781 cgccccgggt gagctgtatg cctacctgaa cagccccggc gtgccgttgc ggctgccggt
      841 cccgcacttc aacgtgctca acggtggggc gcacgccccg aactcgctgg acttccagga
      901 gttcatggtc gctcccatcg gtgcgccctc gctgccggag gcggtgcgtg cgggcgcggg
      961 cgtgtacgcg aagctccgtt ccctgctgca ggcccagggc tttgccaccg gactgggtga
     1021 cgagggcggt ttcgccccgg acatcacccg gccggaggac gtgctggccc tgctggtcga
     1081 ggcgatcgag ggcgccgggt acaccgcggg tcgcgacggc gtggcgatcg cgctggaccc
     1141 ggcggcgagc gagttctaca agggcggggc gtaccacgtg gccggcgagg cgctcagcac
     1201 cgccgacatg atcgaacgct acgccgccat ggtcgcggac tacccggtgt ggtccatcga
     1261 ggacggcctc gccgagggcg actgggatgg ctgggcagcc atgaccgccc agctcgggga
     1321 caaggtccag ctggtgggtg acgacctctt cgtgaccaac ccggccatca tcactgaggg
     1381 catcgccaag aaggtggcga actcggcgct catcaagctc aaccagatcg gctcggtgag
     1441 cgagaccctc gaggcgatgc ggctgtgccg ggaggccggc tacacccaga tggtctccca
     1501 ccgctcgggc gagacggagg actccttcat cgcggacctc gcggtggcca ccggctgcgg
     1561 ccagctcaag accggcgccc ccgcccgcgg cgagcgggtc gcgaagtaca accggctcct
     1621 gcagatcgcg gccacgacgg acctgcccta cgggctcggc tgacacagcg acgacgacgg
     1681 cgggcgcact cccgccgtcg tcgggtctcg gccgttcatc gcggtgcctg ctacgccgat
     1741 gcggcaggat gtgagtgtgc ccgacccgag caccctcacc gccgaccacc ggctcgccac
     1801 ctacgggacc ctgggtccgg ggcgtccgaa ccaccacaag ttggctgggc tcgagcgtcg
     1861 cggagggtgg gcgtgaggcg tcaatctacg tcctcgccct ggacgctggg tgatggtttc
     1921 gggcagttgg cgccctcgat ggcagggctt ccctggatcg gagtcgccaa gtgcgcgatt
     1981 gacggccgat ttccgccctg aatcgggcag ttggcgccct cgatcgct
//
LOCUS       DASRKH010000291         2281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_16343, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000291 DASRKH010000000
VERSION     DASRKH010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16343"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2167)
                     /locus_tag="VFC06_04665"
     CDS             complement(<1..2167)
                     /locus_tag="VFC06_04665"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZL81220.1"
                     /translation="MGEFAVEHAHQRGLKAYYTTPIKALSNQKFGDLRAHYGPDKVGL
                     LTGDTSINPHADIVVMTTEVLRNMIYADSRDLDNLGVVVLDEVHYLADRFRGSVWEEV
                     IIHVGAATALVALSATVSNAEEFGAWLTEVRGDTRVIVSETRPVPLWPHVLLREGLFD
                     LYAPGTDPTDPATAARLNPEIEAIVKRSRQLEQHGAPGRGSYRGRAGPGARPRRSPPR
                     FAVVDQLDAQGLLPAIVFVFSRAGCEDAVEQVRRGGIVLTTEEERDRIRRIVDIRCAG
                     LPPEDLGALGFAAWRDNLEAGLAAHHAGLIPLFKEVVEELFAAGLIQVVYATETLALG
                     INMPARSVVLEKLVKWDGQAHKDLTAGEYTQLTGRAGRRGIDVEGHAVVVDHPGFDAH
                     QLGRLASRRTYPLISSFQPNYNMSINLVATSGVARAREVLEMSFAQFQTDQSVVGSAR
                     RVRELEASLQGFRDAMECDKGDFREYAALRERLGRAEKKTARASSAARKEATAAQLSS
                     LSLGDLVRIGGGRRAGYGVVVLADNNAQAPRPSVVSDSGRLLRLSLADLHSGFDVVGK
                     MSLPRKFQPKDPRARKELGSRVAGEGRAMHQAARAARAPAEVPRTELDGVRQEIRSHP
                     CHTCPDREDHSRWAERYFRTLRDKDRLAGEISRATGSIAAIFDRRCEVLKELGYLEGE
                     AENLSVTPPGGMLRTVYAENDIVIAECLRSGTWDGLNAPA"
BASE COUNT          374 a          795 c          746 g          366 t
ORIGIN      
        1 aggcgggcgc gttcaatccg tcccacgtac cgctccgcag acactccgcg atcacgatgt
       61 cattttccgc gtacacggtg cgcagcatgc cgccaggcgg ggtgacgctc aagttctccg
      121 cctcgccctc gaggtagcca agctccttca gcacttcgca gcggcggtcg aagatcgcag
      181 cgatggagcc ggtggcccgc gaaatctctc cggcgagccg gtccttgtcc ctgagcgtcc
      241 gaaagtagcg ctccgcccac ctggagtggt cctcacggtc cgggcaggtg tgacacgggt
      301 gtgacctgat ctcctggcgt accccatcga gttccgtgcg gggaacctcc gccggagccc
      361 gcgcggcgcg ggcggcctgg tgcatggcgc gaccctcgcc ggcgaccctc gagccgagtt
      421 ccttgcgggc ccgcggatcc ttcggctgga acttgcgcgg cagcgacatc tttcccacca
      481 cgtcgaagcc ggagtgcagg tcggccaacg agagccgcag gagccgaccg gaatcgctga
      541 caacgctcgg gcggggcgct tgtgcgttgt tgtccgcaag gacgacgact ccgtaccctg
      601 cgcggcgacc cccgccgatg cgcacaaggt cgcccaagga cagcgagctg agttgggcag
      661 cggtggcctc tttgcgcgcc gcgctcgacg ccctcgcggt cttcttctcg gcccgaccca
      721 accgctcgcg cagcgccgcg tactcgcgga agtccccctt gtcgcactcc atggcgtccc
      781 gaaacccctg gagggatgcc tccagctcgc gcacacggcg cgcactgccc accacggact
      841 gatccgtctg aaactgggca aacgacatct ccaacacctc gcgggcgcga gcaacgccag
      901 acgtggccac aaggttgatg gacatgttgt agttcggctg gaacgaagag atcagggggt
      961 acgtgcggcg cgacgccaga cgccccagtt ggtgggcgtc gaatccgggg tggtcgacca
     1021 ccacggcgtg cccctccacg tcgatgcccc gcctacctgc gcggcccgtc agttgggtgt
     1081 actcccccgc ggtgaggtcc ttgtgagcct ggccatccca cttcaccaac ttctccagca
     1141 ccacggatcg agccggcatg ttgatgccga gcgccagggt ctccgtcgca tatacgacct
     1201 ggatcagtcc ggcggcgaag agctcctcga cgacctcctt gaacaacgga atcaggcctg
     1261 catggtgggc tgcgagaccc gcctcgaggt tgtcccgcca tgcggcgaag cccagtgcgc
     1321 ccaggtcttc gggaggcagg ccggcgcatc ggatgtccac aatccggcgg atgcggtcgc
     1381 gctcctcctc tgtggtcagc acgatgccgc cgcggcgcac ctgctccacg gcgtcttcgc
     1441 atcccgcacg agaaaagacg aaaacgatcg cgggcagcaa tccttgtgcg tcaagttggt
     1501 cgaccaccgc gaagcgcgga gggctgcgcc gaggcctagc cccgggtcct gcgcggccgc
     1561 gataactgcc tctgccaggt gcaccgtgtt gctccagctg gcgggatcgc ttgacgatgg
     1621 cctcgatctc gggattgagc cgggccgcgg tagcggggtc ggtggggtcg gtgcctggag
     1681 cgtagaggtc gaacagcccc tcccgcagca acacgtgggg ccacagcggc acaggccgcg
     1741 tctcagacac gatgacgcga gtgtcgcccc gcacctccgt cagccaagcg ccaaactcct
     1801 ccgcgttgga gaccgtcgcg gagagcgcca ccagggcggt ggccgcgccc acatggatga
     1861 tgacctcctc ccacaccgag ccccggaagc ggtcggccaa gtagtgcacc tcgtcgagca
     1921 cgaccacgcc aaggttgtcg aggtcccgcg agtccgcgta gatcatgttg cggaggacct
     1981 cggtggtcat caccacgatg tccgcgtggg ggttgatgga cgtgtcgccc gtgagcaggc
     2041 ccaccttgtc cgggccgtag tgcgcgcgca ggtcgccaaa cttctggttg ctcagcgcct
     2101 tgatgggggt cgtgtagtac gccttgagcc cccgttggtg agcgtgttcg accgcgaact
     2161 cgcccaccaa cgggggctca aggcgtacta cacgaccccc atcaaggcgc tgagcaacca
     2221 gaagtttggc gacctgcgcg cgcactacgg cccggacaag gtgggcctgc tcacgggcga
     2281 c
//
LOCUS       DASRKH010000292         2221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_10499, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000292 DASRKH010000000
VERSION     DASRKH010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10499"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VFC06_04670"
     CDS             complement(<1..234)
                     /locus_tag="VFC06_04670"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019617730.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HZL81221.1"
                     /translation="MTPTAKTAPKAPASKTPAKKAPAKKTTAKKAPAKKTTAKKAPAE
                     STPAAEGTAVGRVARVIGPVVDIEFPADGLPGMY"
     gene            complement(252..1145)
                     /locus_tag="VFC06_04675"
     CDS             complement(252..1145)
                     /locus_tag="VFC06_04675"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015881805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HZL81222.1"
                     /translation="MAGQQRVYKAKIKSTQSLKKIFRAMELIAASRIGKARDRVAAAA
                     PYSRAITRAISAVANHSDVEHPLTSERLDTERVAVLVITADRGMAGAYSANAIREAER
                     LAERLEGAGKEIVQYATGRRGVSYYTFRHRELAARWTGDSDGPTHAVASEVAAELLRA
                     FVAPVEEGGVSELHVVYTHFTSMVAQEPRVIRMLPLEIVEGVAELDGDIEPLYDFEPN
                     AEDVLDDLLPRYIESRIYNFMLQSAASELANRQSAMNTAVSNAEDIVRNYTRLANSAR
                     QSEITQEISEIVSGADALAAS"
     gene            complement(1149..>2221)
                     /gene="atpA"
                     /locus_tag="VFC06_04680"
     CDS             complement(1149..>2221)
                     /gene="atpA"
                     /locus_tag="VFC06_04680"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019134872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HZL81223.1"
                     /translation="DSGDPAKQVRCIYVAIGQKGSTIAAVRGALEDAGAMQYTTIVAS
                     PASDPAGFKYLAPYVGSAIGQHWMYGGKHVLIVFDDLSKQAEAYRAVSLLLRRPPGRE
                     AYPGDVFYLHSRLLERCAKLSDEMGAGSMTGLPVIETKANDVSAYIPTNVISITDGQI
                     FLQSDLFNANQRPAIDVGISVSRVGGSAQIKAMKGVSGTLKIDLAQYRSLEAFAMFAS
                     DLDAASRQQLTRGARLMELLKQPQYSPYPIEEQVVSIWAGTKGKLDKIALSDVLRFER
                     ELIDHLRQSTKVMDHILATGKLSEEDEADLHESVDSFVETFLKSANAELTTDASIAGG
                     GEAEVEQEQIVTQRRSTSGAKK"
BASE COUNT          352 a          704 c          742 g          423 t
ORIGIN      
        1 gtacatgccc ggcagtccgt cggcggggaa ctcgatgtcc accacgggtc cgatgacgcg
       61 cgcgacgcga ccgacagcgg tgccttcggc cgcaggcgtg ctctcggccg gggccttctt
      121 ggcggtcgtc tttttcgccg gggccttctt ggcggtcgtc tttttcgccg gcgccttctt
      181 cgccggcgtc tttgacgctg gcgccttcgg agcggtcttt gcggtgggag tcatgggttg
      241 ctctcttccg gtcaggaggc ggcgagcgcg tccgcgcccg acacaatctc gctgatttcc
      301 tgggtgatct cggactggcg cgccgagttg gcgagcctcg tgtagttgcg aacgatgtct
      361 tccgcattcg acaccgcggt gttcatcgcg gactggcggt tggcgagctc ggatgccgcg
      421 gactgcagca tgaagttgta gatgcgggac tcgatgtagc gcgggagcag gtcatcgagc
      481 acgtcttccg cgttgggctc gaagtcgtac agcggctcga tgtctccgtc caactccgcg
      541 actccctcga cgatttcgag gggaagcatg cggatcacgc ggggctcttg agcgaccatc
      601 gaggtgaagt gcgtgtacac cacgtgcagc tccgacacgc caccttcctc gactggtgcg
      661 acgaacgcgc gcaacagttc cgccgcaacc tcgctggcca ccgcgtgcgt cggtccgtcc
      721 gaatcgcccg tccagcgcgc cgcaagttca cggtggcgga acgtgtaata ggacacgccg
      781 cgacggccgg tcgcgtactg gacgatctcc ttgccggccc cctcaaggcg ttcggccaac
      841 cgctcggcct cacggatggc gttggccgaa taggcgcccg ccattccgcg gtcggccgtg
      901 atgacgagca ccgcgacgcg ttcggtgtcg aggcgctcgc tcgtgagtgg gtgctcgacg
      961 tcggagtggt tggcgacagc agagatggcc cgggtgatgg cgcgcgaata cggcgcagca
     1021 gcagccaccc ggtcgcgagc ctttccgatg cgcgaggcag caatcaactc catggcgcgg
     1081 aagatcttct tcagcgactg ggtcgactta atcttcgcct tatagacgcg ctgctggccg
     1141 gccatgagct acttcttcgc gccgctggtg gaacgacgct gcgtgacgat ctgctcctgc
     1201 tcgacctcgg cttcgccacc gccagcgatg ctcgcatcag tcgtcagttc cgcgttggcg
     1261 gacttcagga acgtctccac gaagctgtcg acggactcat gcagatcggc ctcgtcctcc
     1321 tcggacaact tgccggtggc gaggatgtgg tccatgacct tggtcgactg gcgcaagtgg
     1381 tcgatcagtt cacgctcgaa ccgcagcacg tcactcagcg caatcttgtc gagcttgccc
     1441 ttggtgcccg cccagatgga taccacctgc tcctcgatcg ggtacggcga gtattggggc
     1501 tgcttcagca gttccatgag cctggcgccg cgcgtcaact gctgacgcga ggcggcgtcg
     1561 aggtccgaag cgaacatggc gaacgcctca agcgagcggt attgcgccag gtcgatcttc
     1621 aaggttccgg atacgccctt catggccttg atctgcgcgg agcctccgac gcgcgacacc
     1681 gagatgccga cgtcgatcgc agggcgctgg ttggcgttga agaggtcgga ctgcaggaag
     1741 atctggccgt ccgtgatgga aatgacgttg gtcgggatgt acgccgagac gtcgttggcc
     1801 ttggtctcga tgacgggcag tcccgtcatg gaaccggcgc ccatctcatc cgacagcttc
     1861 gcgcaacgct cgagcaagcg ggagtgcagg tagaagacgt cgcccggata agcctcgcgg
     1921 cccggcgggc ggcgcaatag cagcgatacg gcgcgatacg cctcggcctg cttggacagg
     1981 tcgtcaaaga cgatcagcac atgcttgccg ccgtacatcc agtgctgacc gatggccgag
     2041 cctacgtagg gcgcgaggta cttgaagccg gcggggtcgg acgcgggtga cgcgacgatc
     2101 gtcgtgtact gcatggcgcc agcgtcttcg agcgcgccgc gcacggccgc aatggtcgag
     2161 cccttctgac cgatcgccac gtagatgcag cgcacctgct tggcagggtc gccggagtcc
     2221 c
//
LOCUS       DASRKH010000293         2352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_31726, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000293 DASRKH010000000
VERSION     DASRKH010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31726"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VFC06_04685"
     CDS             <1..294
                     /locus_tag="VFC06_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81224.1"
                     /translation="CARAAGIEARADAAAAVAAGRALSVPEALGASLAAIEQVRLEWI
                     DAATAGLPLAWRIVVDDAVFPAAALAEEIDSSVRAVTCPPRDPWKRARPSGPL"
     gene            546..1034
                     /locus_tag="VFC06_04690"
     CDS             546..1034
                     /locus_tag="VFC06_04690"
                     /inference="COORDINATES: protein motif:HMM:NF012651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HZL81225.1"
                     /translation="MNDLTMTVAGWVATEPRHVVGPTGTHLTSFRMASTSRYLDRDKG
                     EWVDGQTEWFTVRVFRGGSIMVKDSIEKGQPVVVQGRFRTNEWESDSGPRTDLIIDAN
                     SLGHDLTRGVAKFTRATGVATLEDPRSRATEVSESDAEVDSAPDAGADAADQPDEASV
                     AA"
     gene            1171..>2352
                     /gene="ettA"
                     /locus_tag="VFC06_04695"
     CDS             1171..>2352
                     /gene="ettA"
                     /locus_tag="VFC06_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003786651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HZL81226.1"
                     /translation="MAEFIYTMYKARKAHGDKVILDDVTLSFMPGAKIGVVGPNGGGK
                     STVLKIMAGLEQASNGEARLSDGYTVGILMQEPELNEDKTVLGNVQEGAGETMAKLKR
                     FNEISDEMSKPDADYDKLLTEMGNLQEDLDHADAWDLDAQLDQAMDALRCPPGDSPVT
                     NLSGGERRRVALCKLLIEKPDLLLLDEPTNHLDAESVLWLEQHLAKYAGAVLAVTHDR
                     YFLDNVAEWILELDRGRAYPYEGNYSTYLEKKSERLAVQGKKDQKLQKRLKEELDWVR
                     SNAKGRQTKSKARLGRYEEMAAEADRTKKLDFEEIQIPPGPRLGNLVLEAKNLKKGFG
                     ERTLIDGLSFTLPRNGIVGVIGPNGVGKTTLFKTIVGLETLDEGELKIGDTVKLSYVD
                     QG"
BASE COUNT          428 a          779 c          804 g          341 t
ORIGIN      
        1 tgtgcccggg ccgccggaat cgaggcgcgg gcggatgctg ccgctgcggt agcggccgga
       61 agggcattgt ctgttccgga ggctttgggc gcgtccctgg ccgccatcga gcaggttcgg
      121 cttgagtgga tcgacgcggc cacggctggc ctgccgctcg cgtggcgcat cgtcgtagac
      181 gacgcagtgt tcccggctgc cgcgctcgcg gaggagatcg actcgtccgt acgcgcggtc
      241 acctgcccgc cgcgcgaccc ttggaagcgc gcgaggccat cgggtccgct gtgatgctgc
      301 acgtggtcga gccgaccgac ctgattcacc aggcgtaccg caacctcgac gaactcactg
      361 agctgtcgta gcaccggtgg gcgcccggca cggcgctgcc ggacgggccc ggtcctagcg
      421 tcgtgcccag gccgcttcag cgtgccgcct ccaccgctcc gtcaaccgcc tgccgcgtcc
      481 ctctccctgc cccgtccact gttgacggac gccgcgaccg cggcaccgca atcgaggagg
      541 acgcaatgaa cgatttgacg atgacggtgg cgggctgggt cgccaccgaa ccgcggcacg
      601 tggtggggcc gacgggcacg cacctgacga gcttccggat ggcgagtacc agccgctacc
      661 tcgaccgtga caagggcgag tgggtggacg gccagaccga gtggttcacg gtgcgcgtgt
      721 tcaggggagg atcgatcatg gtgaaggact cgatcgagaa gggccagccc gtcgtggtgc
      781 aagggcggtt ccgcaccaac gagtgggaat cggactccgg tccccgcacg gacctcatca
      841 tcgacgccaa cagcctgggc cacgacctga cgcgtggggt cgcgaagttc actcgcgcca
      901 cgggagtcgc gacgctggag gaccccaggt cgcgggcgac ggaggtctcg gagagtgatg
      961 cggaagtcga ctcggcgccc gacgcggggg cagacgcggc cgaccagccg gacgaggctt
     1021 cggtcgccgc ctgagcgcca cgcatccggc ggggcgccgc acactgcggc accccgccgc
     1081 acggccgggg ggctgggggt gccgtcaccg cgtaggctgg ggacctgttc cggcggctca
     1141 cgccgcctcg acgattccga cggagtggca gtggctgagt ttatttacac catgtacaag
     1201 gcgcgcaaag cgcacggcga caaggtcatc ctcgatgacg tcacgttgtc gttcatgccg
     1261 ggcgcgaaga tcggcgtggt ggggcccaac ggcggcggta agtccacggt cctcaagatc
     1321 atggcggggc tcgaacaggc ctccaatggc gaggcgcggc tgagcgacgg atacaccgtc
     1381 ggcattctca tgcaggagcc cgaactcaac gaggacaaga ccgtactcgg caacgtgcag
     1441 gagggcgccg gcgagacgat ggcgaagctc aagcgcttca acgagatcag cgacgagatg
     1501 tcgaagccgg acgcggatta cgacaagctc ctcactgaga tgggcaacct ccaggaggat
     1561 ctcgaccacg cggacgcatg ggacctcgac gcccaactcg accaggccat ggacgcgctg
     1621 cgctgccctc ccggcgattc ccccgtgacg aacctctccg gtggcgagcg ccgccgcgtg
     1681 gcgctgtgca agttgctgat cgagaagccc gacctgctgc tcctcgatga gcccaccaac
     1741 cacctcgacg cggaatccgt gctctggctt gagcagcacc tcgccaagta cgcaggcgcc
     1801 gtgctcgccg tgacccacga taggtacttc ctcgacaacg tcgccgagtg gatcctcgaa
     1861 ctcgaccgcg gtcgcgcgta cccgtacgag ggcaactact cgacgtacct cgagaagaag
     1921 tccgagcgcc tcgcggtgca gggcaagaag gaccagaagc ttcagaagcg cctcaaggaa
     1981 gaactcgact gggtgcgctc gaacgcgaag ggtcgccaga ccaagtcgaa ggctcgactg
     2041 gggcgctacg aggagatggc cgccgaggcg gaccgtacca agaagctcga cttcgaagag
     2101 atccagattc ccccgggccc gcgcctcggc aacctggttc tcgaggccaa gaacctcaag
     2161 aagggcttcg gcgaacgcac actcatcgac gggctgtcct tcacccttcc gcgcaacggc
     2221 atcgtcggcg tcattgggcc aaacggcgtc ggcaagacca cgctgttcaa gaccatcgtg
     2281 ggtctcgaga ctctcgacga gggcgagctc aagatcggcg acacggtcaa gctctcgtac
     2341 gttgaccagg gg
//
LOCUS       DASRKH010000294         2031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_11757, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000294 DASRKH010000000
VERSION     DASRKH010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11757"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..765
                     /locus_tag="VFC06_04700"
     CDS             85..765
                     /locus_tag="VFC06_04700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HZL81227.1"
                     /translation="MLLKDSRAFATIRWRQDGFRRAYGSDPSGRTMRNLFGQVDGSAN
                     LQPGSSDFDAAVMAVDDAPSWLRGGTSFVLRRIVMDLDKWDAVDRVGRENTVGRTLDT
                     GAPLTGGREHDPLDLDKKGPNGLPVISSVAHARRAHVDDPAMRIVRLGYSYEDPAPVG
                     SDALSDTGLLFGAWQADVNRQFVPIQRALDEADLLNVWTTPVGSAVFAIPPGCREGGY
                     LGEALLAV"
     gene            complement(754..1371)
                     /locus_tag="VFC06_04705"
     CDS             complement(754..1371)
                     /locus_tag="VFC06_04705"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZL81228.1"
                     /translation="MSAEPSSVPDRRRFNHTIRYQKQLLANLPEGVRTAIDAGCGEGL
                     AARTMARDGLTVTAIDADEASIERARAQDCERITYVVGDIFETDVEPADVVYAGALLH
                     HMDIDAGLERLKSWVAPGGRLCIVGLARATWRDLHREMAGSLAEKAFALAKGSSWEHG
                     SPTVWPPPHTYREVRAASMRILPGAEYKARVLWRYTIVWDRPSDS"
     gene            1450..>2031
                     /locus_tag="VFC06_04710"
     CDS             1450..>2031
                     /locus_tag="VFC06_04710"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HZL81229.1"
                     /translation="MSNVAMPPAEVHIDESLVRALLRGQHPDLAKLPIGSTHEGWDNV
                     TIRIGDKLAVRLPRRRLGAALSATELDWIPTVGAAWTFPTPRALRVGEPNERYPWRWS
                     VVPWLEGSPAYEAPLNADGARDLGAALAQVHQPAPADAPINPFRSRPLSDRAERLDLR
                     LEALEVEHGDKVRAEVARGLFQAGARQPTGPITW"
BASE COUNT          302 a          722 c          696 g          311 t
ORIGIN      
        1 gctgacgact acgaggacgg atccatcacg gcggtgacga tcgtggtctc cacctcgtga
       61 ccgtcgctga cggtcgcgct gcgcatgctg ctcaaggact cgcgtgcgtt cgccaccatc
      121 cggtggcgtc aggacggctt ccgccgggcc tacggatcgg acccgtcagg gcgcaccatg
      181 cgcaacctct tcgggcaggt cgacgggtcg gcgaacctcc agccgggttc gagcgacttc
      241 gacgccgcgg tgatggcggt cgacgatgct ccctcctggc tgcgcggcgg caccagcttc
      301 gtgctgcggc gcatcgtcat ggacctcgac aagtgggacg cggtggatcg ggtggggcgc
      361 gagaacacgg tggggcgcac cctggacact ggcgcgccgc tcacgggcgg gcgcgagcac
      421 gatccgctcg acctggacaa gaaggggccc aacggtctgc ccgtgatctc gtcggtcgcg
      481 cacgccaggc gggctcacgt cgacgacccg gccatgcgca tcgtccgcct gggatacagc
      541 tacgaggacc cggcccctgt cggctcggac gcgctgagcg acaccgggct cctctttggg
      601 gcgtggcagg ccgacgtcaa ccgtcagttt gtgccgatcc agagagcgct cgacgaggcg
      661 gacctcctca acgtctggac cacgccggtc ggctcggcgg tgtttgccat cccgccgggg
      721 tgccgcgagg gcgggtatct cggggaggcg ctgctagctg tctgaggggc gatcccacac
      781 gatcgtgtag cgccagagga cccgcgcctt gtactcggcg cccggcagga tccgcatgct
      841 cgcggcgcgc acctcacggt aggtgtgcgg cggcggccag acagtgggcg agccgtgctc
      901 ccacgacgag ccctttgcca gcgcaaacgc cttctccgcg agcgaccctg ccatctctcg
      961 gtggaggtcc cgccaggtgg cgcgcgccaa gcccacgatg cacaagcgac cgccgggtgc
     1021 gacccacgac ttgaggcgtt ccaagccggc gtcgatatcc atgtggtgca gtagggcacc
     1081 cgcatagacc acgtcggccg gctcgacatc ggtctcgaag atgtcgccga ccacgtaggt
     1141 gatgcgctcg cagtcctgcg cgcgggcacg ctcgatgctc gcctcatcgg cgtcaatggc
     1201 ggtcaccgtc aggccgtcgc gagccatggt cctggccgcc aaaccctcgc cacagcccgc
     1261 atcgatcgcg gtgcggacgc cctccgggag gttcgctagc agttgcttct gatagcgaat
     1321 cgtgtggttg aaacggcggc ggtccggaac ggacgaaggc tctgctgaca tgcccccagt
     1381 gtgccctcga tcgcgctctc cgcttcccgc cgcctcgcac gccgcggtga gagtccctag
     1441 tctggacggg tgagcaacgt tgccatgcct cctgctgagg tacacattga cgaaagcctg
     1501 gtgcgcgccc tgttgcgcgg tcaacacccc gaccttgcga aactcccgat cggctcgaca
     1561 cacgagggct gggacaacgt caccatccgc atcggcgaca aactcgccgt ccggctcccg
     1621 cgccgacgcc tcggggcggc cctctccgca accgaactcg actggattcc gaccgtcggt
     1681 gccgcgtgga ccttccccac gccgcgagcg ctgcgcgtgg gagagccgaa cgagcgctac
     1741 ccgtggcggt ggtccgtcgt tccgtggctt gagggaagcc ccgcctacga ggcgccgctc
     1801 aacgccgacg gcgcgcgtga cctgggtgcc gcgctggcgc aggtgcatca gcctgccccg
     1861 gccgacgccc ccatcaatcc gttccggtcg cggccgctct cggaccgagc cgagcgactc
     1921 gatctacgcc tcgaggccct cgaggtcgag cacggcgaca aggtccgcgc ggaggtggcg
     1981 cggggactgt tccaggcggg cgcgcggcag ccgacggggc ccatcacctg g
//
LOCUS       DASRKH010000295         2376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_59634, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000295 DASRKH010000000
VERSION     DASRKH010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59634"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="VFC06_04715"
     CDS             <1..659
                     /locus_tag="VFC06_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81230.1"
                     /translation="LRAERPVAVIEVKLLSDLGPQQLTRYLAAFPDVGTYCVLHLGGL
                     PVNLRSAHPWRSLTWEAVLDAYAASHNAWVSTTARAWRAQLAPLVPVVDATTVWNDVP
                     DDGAGMELALRARVAWLSRRMDSWCEVDHDLDPSSGGGNWAVRMWAATSTPHHFVIAE
                     IQEGMTAYEWKPDPARPYRQRLRGPVVLLGVLQDSVTTSAGFDWSLLLEDLESGVYFA
                     "
     gene            complement(684..1073)
                     /locus_tag="VFC06_04720"
     CDS             complement(684..1073)
                     /locus_tag="VFC06_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZL81231.1"
                     /translation="MLTIRLATYLVRDLDEAMRFFLDALGFELREDVTHEDGGRWVVI
                     GPPGFGTGLHLVVAAPGTDLPTAQAGGDVAFILGTDDFAGRHRIMDAHGVRFLEEPRH
                     EPYGTVAVFADPAGVRWDLLEPITPNA"
     gene            complement(1291..1779)
                     /locus_tag="VFC06_04725"
     CDS             complement(1291..1779)
                     /locus_tag="VFC06_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HZL81232.1"
                     /translation="MTVISTTPDTATLTLTIVAGLAAPPDRAWQIWVDPRQLERWWGP
                     PTWPATFVQHDVVVDGRSSYYMTGPDGEKAHGWWRFLSVDEPHSLEFEDGFADDAGTP
                     NPDMPTTHARVDLHGTADGTRMTVTSRFTTLEQMERLVAMGMVEGMTLAMGQIDDILV
                     GV"
     gene            complement(1776..2126)
                     /locus_tag="VFC06_04730"
     CDS             complement(1776..2126)
                     /locus_tag="VFC06_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZL81233.1"
                     /translation="MVTRHAEREATDQIFAALSDATRRDIVTRVLRDEASVSTLARHY
                     DMSFAAVQKHVAVLERAHLVTKHRRGREQIVSGEVTTIRTAARLLGEYEEIWRGRIDR
                     IDEILAEPAEGAAP"
BASE COUNT          341 a          793 c          854 g          388 t
ORIGIN      
        1 tcctgcgcgc cgagaggccc gtcgccgtga tcgaggtcaa gctcctgtcc gatcttggac
       61 cgcaacagct gactcggtac ctggcagcct tcccggacgt cggcacgtac tgcgtgctgc
      121 atctcggtgg gctgcccgtc aacctgcgca gcgcgcaccc gtggagatcg ctgacgtggg
      181 aggcggtcct tgacgcctac gccgcgagtc acaacgcatg ggtctcgacc acggcgcgcg
      241 cctggcgggc gcaactcgca ccgctggttc ccgtggtcga cgcgaccacc gtgtggaatg
      301 acgttcccga cgacggcgcg gggatggagc tggccctacg cgcccgggtt gcctggctct
      361 ctcgacggat ggactcgtgg tgtgaggtgg atcacgacct cgacccgtca tccggcggcg
      421 ggaactgggc ggtccgcatg tgggcggcta cgtcgacgcc ccatcacttc gtcatcgcgg
      481 agatccagga agggatgacc gcgtacgagt ggaagccgga cccggctcgc ccgtaccgcc
      541 agcggctgag ggggccggtc gtgctgctcg gcgtgctgca agactccgtt acgacctccg
      601 caggtttcga ctggtcgctg ctcctcgagg acctcgagtc aggcgtctac ttcgcgtagg
      661 cgcatccccg acgtcgaggg acgtcaggcg ttcggggtga tcggctcgag caggtcccag
      721 cggacgcccg ccggatcggc gaagactgcg acggtcccgt acggctcgtg acggggctcc
      781 tcgaggaacc ggacgccgtg cgcgtccatg atccggtggc gcccggcgaa gtcgtccgtc
      841 ccgaggatga aggcgacgtc gccgcccgcc tgcgcggtcg ggagatcggt gccgggcgcc
      901 gcgacgacga ggtgcagccc cgtcccgaac ccgggcgggc cgataacgac ccaacgcccg
      961 ccgtcctcgt gcgtgacgtc ctcgcgcagc tcaaagccca gggcgtcgag gaagaagcgc
     1021 atcgcctcgt cgaggtcacg cacgaggtag gtcgccagcc ggatcgtcag caccctcgga
     1081 tcgtagccgc cggtgcgtag tccagcctgc tcgcctcccg cggcggcgcc aaccacgaca
     1141 tcggggatct ctacgcgctc gttccaacca gcgagctcgt tcccgaggtc acggagatcg
     1201 gcttcgacga catcccgcag ggtctcgccg acctcgccgc caacaacgtc accgggcggc
     1261 gcgctgctac agagctgggc tcacgggccg tcagacgccg accaggatgt cgtcgatctg
     1321 gcccatcgcg agcgtcatgc cctcgaccat gcccatcgcc acgagccgtt ccatctgctc
     1381 gagggtcgtg aaccgcgacg tgacggtcat gcgcgtgccg tcggcggtcc cgtgcaggtc
     1441 gaccctggca tgggtcgtcg gcatgtcagg gttgggcgtc cctgcgtcgt cggcgaagcc
     1501 gtcctcgaac tcgagcgagt gcggttcgtc gacggacagg aagcgccacc acccgtgggc
     1561 cttctcgccg tcgggcccgg tcatgtagta gctggagcgc ccatcgacga cgacgtcgtg
     1621 ctgcacgaag gtcgcgggcc aggtgggcgg gccccaccac cgctcgagct gacgcgggtc
     1681 gacccagatc tgccacgccc ggtcgggcgg ggcggcgaga ccggccacga tggtcagggt
     1741 cagtgtcgcg gtgtctggcg tggtgctgat gacggtcatg gtgctgctcc ttcggcgggc
     1801 tcggcgagga tctcgtcgat tcggtcgatg cggccgcgcc agatctcctc gtactcgccg
     1861 agcagacgtg cggccgtgcg gatggtggtg acctcgcccg acacgatctg ctcgcggccg
     1921 cggcggtgct tggtcacgag gtgcgcgcgc tcgagcaccg cgacgtgctt ctgcacggcg
     1981 gcgaagctca tgtcgtagtg ccgggcgagg gtggagaccg aggcttcgtc acgcaggacg
     2041 cgcgtgacga tgtctcggcg ggtcgcgtcg gagagggcgg cgaagatctg gtccgtcgcc
     2101 tctcgttccg cgtgtcgtgt aaccatgtgg ttgtacgtta cgcgccgcgt cggctcgccg
     2161 caaggggtgg gggcccaggc cgacggtgcc gcgcaacgag cgctacatcg agaggttgcc
     2221 gatctgtcct ggttcaggga tggtggaccg gtggtctcaa tggatgagga tcaggcgatc
     2281 gcgcacgtgc aggatcggct gatcgagcgg tttcccgacg tcggcacgga agcggttcgg
     2341 ctcgcggtgc aggacgtcca caaccggttc gacggg
//
LOCUS       DASRKH010000296         4834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_4984, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000296 DASRKH010000000
VERSION     DASRKH010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4984"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="VFC06_04735"
     CDS             complement(<1..496)
                     /locus_tag="VFC06_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZL81234.1"
                     /translation="MTPSAITVSGLTKHYGDKHAVDGLDLEVFTGEVFALLGPNGAGK
                     STTVEILEGFRKRTAGEVSVLGVDPQHATSHWRERVGVMLQATSMRSLLSAREALIHA
                     ASQYPHPRDVDETLEAVGLTDKANAKPQTLSGGQRRRLDVALAIIGSPELLFLDEPTT
                     GFDPE"
     gene            complement(572..1789)
                     /locus_tag="VFC06_04740"
     CDS             complement(572..1789)
                     /locus_tag="VFC06_04740"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006590706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HZL81235.1"
                     /translation="MTSPARRVSARLGAIAPSATLAVDAKAKALKAAGRPVIGFGAGE
                     PDFPTPDFIVEAAVAAARDPKNHRYTPAAGLPDLKQAIAAKTLRDSGYEIDPAHVVVT
                     NGGKQAVFQAFASIVDPGDEVLLPAPYWTTYPEAISLAGGVPIEVFAGADQGYLVSVE
                     QLERARTDKTKALLFCSPSNPTGAVYSPAQTKAIGEWALEHGIWVITDEIYEHLTYDD
                     AVFTPIVRAVPGLAEQAIVVNGVAKTYAMTGWRVGWMIAPADVTKAAANFQSHLTSNV
                     ANVSQRAAIAALEGGLDDVHAMRAAFDRRRKIMVSMLGEIEGLKIPTPKGAFYAYPSV
                     AGLLGLTVRGRRITSSADLAAVILEEAEVAVVPGEAFGPSGYLRLSYALGDDDLVEGV
                     TRMQKLLGEAKAK"
     gene            1982..2054
                     /locus_tag="VFC06_04745"
     tRNA            1982..2054
                     /locus_tag="VFC06_04745"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2015..2017,aa:Trp,seq:cca)
     gene            2101..2358
                     /gene="secE"
                     /locus_tag="VFC06_04750"
     CDS             2101..2358
                     /gene="secE"
                     /locus_tag="VFC06_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015937869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HZL81236.1"
                     /translation="MSESAVTDSGERDPRHEEREGLNIFGRIALFVRQVISELRRVVT
                     PTREELINYIWVVLGFVGVMMTLTFLLDLGFNSLTKWVFGD"
     gene            2385..3263
                     /gene="nusG"
                     /locus_tag="VFC06_04755"
     CDS             2385..3263
                     /gene="nusG"
                     /locus_tag="VFC06_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HZL81237.1"
                     /translation="MSTDPHDAVETVDDAEVAPEQVAPVDDVAIDDAPVDAVVESDDD
                     TVPSEPVAAMEEDALTVPDEPSSDADVEDALTATDEPAVDAGVEVEDEIDPKVAFRSS
                     LLMQDGDWYVVHSYSGHEKRVKQAIEHRVVSLNMEDYIFQVEVPMEDVVEYKNGQKKQ
                     VTRVRMPGYVLVRMELGDASWGAVRNTPGVTGFVGHAHQPVPLTPDEVYSMMAPPAPA
                     SAASAEGGDSGSGPRIELDFQVGEAITVTDGPFATLPGTISEINVESQKLHVLVSIFG
                     RETPVELSFAQVQKID"
     gene            3340..3771
                     /gene="rplK"
                     /locus_tag="VFC06_04760"
     CDS             3340..3771
                     /gene="rplK"
                     /locus_tag="VFC06_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017833272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HZL81238.1"
                     /translation="MAPPKKKVAGLIKLQIQAGAANPAPPVGPALGQHGVNIMEFCKA
                     YNAATESQRGNIVPVEITVYEDRSFTFITKTPPASQLIKKAAGVAKGSGVPHTNKVGS
                     LTQAQVREIAEMKKDDLNANDIDAAAKIIAGTARSMGITVK"
     gene            3837..4532
                     /gene="rplA"
                     /locus_tag="VFC06_04765"
     CDS             3837..4532
                     /gene="rplA"
                     /locus_tag="VFC06_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HZL81239.1"
                     /translation="MKRSKAYRDGEQLIDKNQFYTPLEALRIARKTSSKKADSTIDIV
                     FKLGVDPRHADQMVRSTVILPHGTGKTSRVLVFANGDKADAAREAGADIVGGDELIEE
                     VQNGRQDFDAVVATPDLMGKVGRLGRVLGPRGLMPNPKTGTVTMDVTKAVSDIKGGKI
                     EFRVDKHANLHTVLGKASFGDQKLLENYQTLIDEVLRSKPAASKGKYIIKAAISATNG
                     PGIPLDATAKIEA"
BASE COUNT          903 a         1540 c         1573 g          818 t
ORIGIN      
        1 cctcggggtc gaagccggtg gtcggctcgt ccaggaagag gagctcgggg ctgccgatga
       61 tcgcaagtgc gacatcgagt cgacgccgct gcccaccaga cagcgtctgc ggcttggcgt
      121 tggccttgtc ggtgaggccc accgcctcaa gcgtttcgtc gacgtcgcgc gggtgggggt
      181 attgcgaggc ggcatgaatc agcgcctcgc gagcactgag cagtgaacgc atggaagtgg
      241 cctgcagcat gacgcccacg cgctcgcgcc aatggctggt ggcatgctgc ggatctacgc
      301 ccagcacgct cacctcgccg gccgtgcgct tgcggaaacc ctcaaggatt tccaccgtgg
      361 tcgacttgcc cgctccgttg ggtcccaaca gggcgaacac ctcgcccgtg aagacctcga
      421 ggtcgagccc atcgacggcg tgcttgtcgc cgtagtgctt ggtgagcccg gaaacagtga
      481 tggccgatgg cgtcatggca cgaccgtacc gggcgtcaac tgatggcgca cctgtttctt
      541 gagcccgatg agcgcactag gttcggcgac gctatttggc cttggcctcg cccaacagct
      601 tctgcatccg cgtgacgccc tccaccaggt cgtcgtctcc gagcgcgtag ctcagtcgca
      661 ggtagccgct ggggccgaac gcctcgccgg gaaccaccgc cacttcggcc tcttccagga
      721 tgacggcggc aaggtccgcg ctggaggtga tccggcgccc gcgcacggtg agaccgagca
      781 agcccgcaac ggacgggtac gcgtagaacg ctcccttcgg cgtcgggatc ttaagtccct
      841 cgatctctcc cagcatcgaa accatgatct tgcgcctgcg gtcaaacgcg gcgcgcatcg
      901 cgtgcacgtc gtccagtccg ccctcgagag ccgcgatggc ggcgcgttga gacacgttcg
      961 ccacgttgct cgtgaggtgg gactggaagt tggcggcggc cttggtgacg tcggcgggcg
     1021 caatcatcca tcccacgcgc cagccggtca tggcgtacgt cttggcgact ccgttgacga
     1081 cgatggcctg ctcggcgagg ccgggaacgg cgcgcacgat gggagtgaac accgcgtcgt
     1141 cgtacgtgag gtgttcgtag atctcgtcgg tgatgaccca gatgccgtgc tcgagggccc
     1201 actcgccgat cgccttggtc tgcgcggggg agtagacagc tccggtgggg ttggagggcg
     1261 aacagaacag cagcgccttc gtcttgtcgg tgcgcgcccg ctcgagctgc tcgacggaca
     1321 caaggtatcc ctgatcggcg cccgcgaata cctcgatggg gactccgcca gcgagcgaga
     1381 tggcctcggg gtaggtggtc cagtacgggg caggaagcag cacctcgtcg cccgggtcga
     1441 cgattgatgc gaaggcctgg aacacggcct gcttgccacc gttggtgacg acgacgtgcg
     1501 ccgggtcgat ctcgtagccg ctgtcgcgca gtgtcttcgc cgcgatcgcc tgcttgaggt
     1561 ccggcaagcc cgccgccggc gtgtagcggt ggttcttggg gtcgcgtgcc gccgcgacgg
     1621 cggcctcgac gatgaagtcg ggcgtgggga agtcgggctc cccggcgcca aacccgatga
     1681 cgggtcggcc agcggccttg agggccttag ccttggcatc gacagcaagg gtggcggagg
     1741 gcgcgatggc gccgaggcgg gcagagacgc ggcgtgcggg tgaggtcatg cgtcccattg
     1801 ttccacctag cgcgcggcgc cgatgcgacc gttcgcgttc gggtcccaaa ccgcgtaaca
     1861 tggcacttcg gcgattgaca gaagccatga tgactcgcgc ccaaaatgcg ggtcagctga
     1921 tgtcctcctt gtggggggcg gctctctatt gagaaccaca tggggttcac aaccaccccg
     1981 aagggcagtg gcgcaattgg tagcgcatcg gtctccaaaa ccggcggttg ggggttcgag
     2041 tccctcctgc cctgcgtaga gcgggacttc ccgcaccaca caagcaaagg aattatcaag
     2101 gtgagcgaat ctgcagtgac ggattccgga gaacgggacc cgcgccacga ggagcgtgaa
     2161 ggtctcaaca tcttcgggcg catcgcgttg ttcgtgcgcc aggtgatttc ggaacttcgt
     2221 cgcgtggtca ctcctacacg cgaagaactc atcaactaca tctgggtcgt gctcggattc
     2281 gttggcgtca tgatgacgct gacgttcctg ctagacctcg gcttcaactc gctgaccaag
     2341 tgggtcttcg gcgactagcc gagtagccag acgaaaggaa agacgtgagc acagatcccc
     2401 acgacgccgt cgagaccgtc gacgacgccg aggtcgcgcc cgagcaggtt gcgcccgtgg
     2461 acgacgttgc catcgacgac gctccagtgg acgcggtggt cgagagcgac gacgacaccg
     2521 tgccctccga gcccgtagcg gcgatggagg aggacgcgct caccgtgccc gacgagcctt
     2581 cgagtgacgc agacgtcgag gacgccctca ccgcgaccga cgagcctgca gttgacgccg
     2641 gcgttgaggt tgaggacgag atcgacccca aggtcgcctt ccgctcctcg ttgctgatgc
     2701 aggacggcga ctggtacgtc gttcacagtt acagcggcca cgagaagcgc gtcaagcagg
     2761 ccatcgaaca ccgcgtggtg agcctcaaca tggaggacta catcttccag gtcgaggtcc
     2821 ccatggaaga cgttgtcgag tacaagaacg gccagaagaa gcaggtcacc cgcgtgcgga
     2881 tgcccggcta cgtgctggtg cgcatggaac tcggtgatgc ctcgtggggc gcggtgcgca
     2941 acacgcctgg tgtgactggc tttgtgggtc acgcccacca gcccgtaccg ctcactcccg
     3001 acgaggtcta ctcgatgatg gcgccgccgg cacccgcatc tgccgcgtcg gccgaaggcg
     3061 gcgactccgg ctccggccct cgcatcgaac tcgacttcca ggtgggagaa gcgatcaccg
     3121 tcaccgacgg cccgttcgca acgctgcctg gcacaatttc cgagatcaac gtggagagcc
     3181 agaagctcca cgtcctcgta tcaatcttcg gccgcgagac tccggtcgaa ctctcgttcg
     3241 cgcaggttca gaagatcgac tagtcgttac tgaccggcaa caacgaacca ccaggcggtg
     3301 tgagccgcca ccacttacac agaaggaccc gaaagaccaa tggcacctcc taagaagaag
     3361 gttgccggcc tgatcaagct ccaaattcag gctggtgccg cgaacccggc tccgcccgtc
     3421 ggccctgcat tgggtcagca cggcgtgaac atcatggagt tctgcaaggc ctacaacgcc
     3481 gcgaccgagt cgcagcgagg caacatcgtc cccgtggaaa tcacggtgta cgaggaccgc
     3541 agcttcacgt tcatcaccaa gaccccgccc gcgtcgcagc tcatcaagaa ggctgctggc
     3601 gttgcaaagg gttccggtgt cccgcacacc aacaaggtcg gctcgctgac ccaggctcag
     3661 gtgcgcgaga tcgccgagat gaagaaggac gacctcaacg cgaatgacat cgacgccgcc
     3721 gccaagatca ttgctggcac cgcgcgctcg atgggcatca ccgttaagta gttgacacac
     3781 cccagtggga gggccagcgc tggcccgtca ccacgacttc gagaaagaag aagcacatga
     3841 agcgctccaa ggcctatcgc gatggcgaac agctgattga caagaaccag ttttataccc
     3901 cgctcgaggc ccttcgcatt gctcggaaga cctcgtccaa gaaggccgac tcgacgatcg
     3961 acatcgtctt caagctcggc gtcgacccgc gtcacgcgga ccagatggtc cgatccaccg
     4021 tcatcctgcc tcacggcacc ggcaagacct cccgggtcct ggtcttcgcc aacggtgaca
     4081 aggcagatgc ggctcgcgag gcgggcgcag acatcgtcgg tggcgacgaa ctgatcgaag
     4141 aggtgcagaa cggccgtcag gacttcgacg ccgtcgtggc cacgcccgac ctcatgggca
     4201 aggtgggacg cctgggccga gtgctcggtc cccgtggcct catgccgaac cccaagaccg
     4261 gcaccgtgac gatggacgtg accaaggccg tgtccgacat caagggtgga aagatcgagt
     4321 tccgcgtcga caagcacgcc aacctgcaca ctgtgctggg caaggcgtcg ttcggcgacc
     4381 agaagctgct cgagaactac cagaccctca tcgacgaggt gctgcgttcc aagcctgccg
     4441 cgtccaaggg caagtacatc atcaaggccg cgatctcggc cacgaacggc cccggcattc
     4501 cgctggacgc caccgccaag atcgaggcat agctccccgc gcacagcgcg atgcgaaggc
     4561 ccggttccca ctatgggggc cgggccttcg tgctgccgca cgcgccgcct tgatgcaggt
     4621 cgaggtgcct cactgagaag tgagcgcgag agttgatctg gtgcctcacg caaggtgagc
     4681 gcgagggctt gcagctattt cgttgtctgg agtcgacgga tcgacgcttc caaggatgcc
     4741 atgttcaggt gccggctggt ggtcatggcc tcggttttgt cgcgggatag ctcggtcagc
     4801 cgaagctgga tctggttgat ccgcctggtc agat
//
LOCUS       DASRKH010000297         2387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_35098, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000297 DASRKH010000000
VERSION     DASRKH010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35098"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VFC06_04770"
     CDS             <1..404
                     /locus_tag="VFC06_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZL81240.1"
                     /translation="LSLRRIGLPAAERAARAHEILARFGLAGHAEVPVHSLSGGQKQL
                     LALACVLATEPAVLVCDEPTTLLDLRWRRVVDELLDGLDQQVLQVTHDLDAAARAERV
                     LVVDAGAVVFDGDPGTAVDAYRALMAPRVSP"
     gene            401..1042
                     /locus_tag="VFC06_04775"
     CDS             401..1042
                     /locus_tag="VFC06_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HZL81241.1"
                     /translation="MTRRAGPALRGPWAGPLGLYRPGATVLHRAGPGAKLGALAALGV
                     LVVVLRGPWSAVALLVAVVVAAALGRLPLRSTARSTLPVLLTATLLGAYQWWARGWQV
                     GAEVAVDLVTLVLAATVVTATTPADRMLDALGTAARPLRHVGLAPETVALAVGIMLRS
                     IPALLATFAETRDAARARGLDRSPRALLVPAAVRTVARARATGDALAARGLAE"
     gene            1187..1255
                     /locus_tag="VFC06_04780"
                     /pseudo
     CDS             1187..1255
                     /locus_tag="VFC06_04780"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1266..1589)
                     /locus_tag="VFC06_04785"
     CDS             complement(1266..1589)
                     /locus_tag="VFC06_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HZL81242.1"
                     /translation="MTEPRFDEVVHAPHRLRILALLAAVAGQVEFAILRDLLDVADSV
                     LSKHLKVLHDCGYVELRKPTGQGRVRTWVQLTTRGHDAYAAHVAALREIVERGPGEAV
                     GDSPR"
     gene            complement(1586..2029)
                     /locus_tag="VFC06_04790"
     CDS             complement(1586..2029)
                     /locus_tag="VFC06_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81243.1"
                     /translation="METDAERQLALIADTRARAADRLVTPWWYHVALGVLMGTLVLAV
                     GLGFGTWWYFVVVALVLVGEGVVVGAYRRVMGVWTTTWYAFPRWAIWIGFGVGLALVS
                     AAAAVRLSTVIVWPVWVLAAVMFVHMVVLGRWTDAVWRARLHADA"
     gene            complement(2075..>2387)
                     /locus_tag="VFC06_04795"
     CDS             complement(2075..>2387)
                     /locus_tag="VFC06_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HZL81244.1"
                     /translation="SSTTAADVEEALHDVIDPELGINVVDLGLVYGVMVDQQNHAVID
                     MTLTSAACPLTDVIEDQAGMSLEGLVDGFRINWVWMPPWGPEKITPDGREQMRALGFN
                     I"
BASE COUNT          332 a          888 c          834 g          333 t
ORIGIN      
        1 cgctctccct gcgtcggatc ggtctgccgg ccgcggagcg cgccgcgcgc gcccacgaga
       61 tcctggcccg gttcggactc gccgggcacg ctgaggtccc tgtgcactca ctctcgggcg
      121 gccagaagca gctgctcgcc ctcgcctgcg tcctcgcgac ggaacccgcg gtgctcgtgt
      181 gcgacgagcc gacgaccctg ctcgacctgc gctggcgcag ggtcgtcgac gagctgctcg
      241 acgggctgga ccagcaggtg ctccaggtga cgcacgacct cgacgccgcg gcccgggccg
      301 agcgggtcct cgtcgtcgac gcgggcgctg tggtgttcga cggcgacccg ggcacggccg
      361 tcgacgccta ccgcgcgctg atggcaccgc gggtgtcccc atgacccgcc gtgcgggccc
      421 ggcgctgcgg ggtccgtggg ccgggccgct cgggctgtac cggcccggcg cgacggtgct
      481 gcaccgggcc gggccaggcg ccaagctcgg cgcgctcgcc gcgctgggag tgctcgtcgt
      541 ggtcctccgg gggccatgga gcgccgtcgc cctgctcgtc gccgtcgtgg tcgcggccgc
      601 gctcggccga ctcccgctgc ggtcgacggc tcgcagcacc ctgcccgtgc tgctcaccgc
      661 gacactgctc ggcgcctacc agtggtgggc acgcggctgg caggtcggcg cggaggtcgc
      721 tgtcgacctc gtgaccctcg tcctcgcggc cacggtcgtg accgcgacca ccccggccga
      781 ccgcatgctc gatgcgctcg gcacggcagc tcggccgctg cgtcacgtcg ggctggcgcc
      841 cgagaccgtc gccctagccg tcgggatcat gctccgctcg atcccggcac tcctcgcgac
      901 gttcgccgag acccgagacg ccgcacgtgc ccgtgggctg gatcggtccc ctcgcgcgct
      961 gctcgtcccc gccgcagtcc gcacggtcgc gcgcgcccgc gcgaccggcg acgcgctcgc
     1021 cgctcgcggc ctcgccgagt gatcccaccc cggggccggc tgccctcgcc gtgcggtcgc
     1081 cagatggctg acgttacttc tgccccatga ccttcaatga gggtggcagc ttcgagggcg
     1141 gccgggtcaa gacaagtcgc ggcatgcctg aacccccgac acctggacgc acgggtcgag
     1201 cgcggaacgc cagcgctggt tcatgaccgg ctacaccaag agcacgatcg tggcgtgcga
     1261 cacgttcagc gcggcgagtc tccgaccgcc tcgcccgggc cgcgctcgac gatctcgcgc
     1321 agggccgcga cgtgggccgc atacgcgtcg tggccgcgcg tcgtgagctg gacccacgtg
     1381 cgcacccggc cctggccggt gggcttgcgc agctcgacat acccgcagtc gtgcaggacc
     1441 ttgaggtgct tgctgagcac ggagtcggcg acgtcgagca ggtcgcgcag gatcgcgaac
     1501 tcgacctgac cggcgacggc cgcgagcagg gccaggatgc gcaggcggtg aggcgcgtgc
     1561 acgacctcgt cgaagcgggg ctcggtcacg cgtcggcatg cagtcgcgcg cgccagacgg
     1621 catccgtcca gcgacccagc acgaccatgt gcacgaacat cacagcggcc agcacccaga
     1681 ccggccagac gatcacggtc gagagcctca ccgcggcggc tgcgctcacc agcgcgagac
     1741 ccacgccgaa cccgatccag atggcccagc gcgggaatgc gtaccaggtc gtcgtccaga
     1801 ctcccatcac gcgtcggtag gcgcccacga cgaccccctc gcccaccagg acgagggcga
     1861 ccacgacgaa gtaccaccag gtcccgaagc cgaggccgac cgccaggacg agagtgccca
     1921 tgagcacgcc gagcgcgacg tggtaccacc agggcgtgac cagtcggtcg gccgcgcgag
     1981 cccgcgtgtc ggcgatgagg gcgagctggc gctccgcatc tgtttccatg acggaaagac
     2041 aatcactttc cgtcatggaa tgtcaaccag ggcctcagat gttgaagccg agtgctcgca
     2101 tctgctcgcg cccgtcgggc gtgatcttct cgggacccca cggcggcatc cacacccagt
     2161 tgatgcggaa cccgtcgacg agcccctcga gcgacatgcc ggcttggtcc tcgatgacgt
     2221 ccgtgagcgg gcaggccgcc gatgtcaggg tcatgtcgat gacggcatgg ttctgctggt
     2281 cgaccatgac gccgtagacg aggcccaggt cgacgacgtt gatgccgagc tcagggtcga
     2341 tgacgtcgtg gagggcctcc tcgacgtccg cggctgtggt cgacgac
//
LOCUS       DASRKH010000298         2358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_58665, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000298 DASRKH010000000
VERSION     DASRKH010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58665"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="VFC06_04800"
     CDS             complement(<1..769)
                     /locus_tag="VFC06_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HZL81245.1"
                     /translation="MAGVLDGKATAATIKAELAERVAALAERGIVPGLGTLLVGSDPG
                     STWYVNGKHDDCAEVGITSLREGLPETATQADIEAAVQRLNDNPACTGFIVQLPLPAG
                     IDTNRVLELVDPDKDADGLHPTNLGRLVLRVGEPVTSPLPCTPKGIIELIERHGVSLA
                     GKHVVVVGRGTTVGRSIGLLLTRKAVNATVTLCHTGTVDLASHTRRADVIVAAAGVRG
                     IISADMVTDGVVLIDVGVSRITDPETGKSKVAGDIAAD"
     gene            complement(770..2062)
                     /gene="glyA"
                     /locus_tag="VFC06_04805"
     CDS             complement(770..2062)
                     /gene="glyA"
                     /locus_tag="VFC06_04805"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005274480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HZL81246.1"
                     /translation="MTSQTPLETSLLDQPLSQVDPEIAAVLEGELTRQRETLEMIASE
                     NFVPRAVLQAQGSVLTNKYAEGYPGRRYYGGCEEVDVAESIAIDRAKSLFGAEYANVQ
                     PHSGATANAAVLHALINPGQKIMGLSLAHGGHLTHGMKLNFSGKLYEVAAYGLNEQSH
                     RIEMEQVRATALAERPDVIIAGWSAYPRHLDFAAFREIADEVGAKLWTDMAHFAGLVA
                     AHLHPSPVPHSDVVSTTVHKTLGGPRSGLIVARDMELAKKLNSAVFPGQQGGPLMHVI
                     AAKATALKIAAGAEFKERQERVLEGARILAERLTAPDAIEAGINVVTGGTDVHLVLVD
                     LRNSPIDGQQAQDLLHDVGITVNRNAVPFDPRPPMVTSGVRIGTPALATRGFAAAEFS
                     EVADIIATALKGDADIAALRARVAKLTGAFPLYPGLVQ"
     regulatory      complement(2124..2211)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01750"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ZMP/ZTP riboswitches appear to regulate genes
                     involved in one-carbon metabolism; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="5-aminoimidazole-4-carboxamide
                     ribonucleotide (ZMP)/
                     5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl
                     5'-triphosphate (ZMT)"
                     /db_xref="RFAM:RF01750"
BASE COUNT          348 a          804 c          821 g          385 t
ORIGIN      
        1 cgtctgcggc gatgtcgcca gcgaccttcg acttgccggt ctcgggatcc gtgatccggg
       61 acacgcccac gtcaatcaac acgacgccgt ccgtgaccat gtccgccgag atgattcccc
      121 gcacgcctgc ggcggcgacg atgacgtcgg cccggcgggt gtgtgaggcg aggtctacgg
      181 tgcccgtgtg gcacagcgtc acggtcgcgt tgacggcctt gcgcgtgagc agcagcccga
      241 tgctgcgccc caccgtggtg ccacgcccca cgaccacgac gtgcttgccc gcaagcgaca
      301 ccccgtgccg ctcgatgagc tcgatgatgc ccttcggcgt gcaaggaagc gggctcgtga
      361 ccggctcacc gacgcgcaac acgagtcgac ccaagttcgt cgggtgaagg ccgtccgcat
      421 ccttgtccgg gtcaacaagt tcgaggaccc tgttggtgtc gattcccgcg ggcaggggca
      481 gctgcacgat gaatcctgtg cacgccggat tgtcgttgag ccgctggacc gccgcctcga
      541 tgtcggcctg agtggcggtc tccgggaggc cctcacgaag agaggtgatg cccacctcgg
      601 cgcaatcatc gtgcttgccg ttgacgtacc aggtggagcc ggggtcgctt cccaccagca
      661 gggtgcccag gcctgggacg atgccgcgct cggcgagcgc cgcgaccctc tcagccagtt
      721 cggccttgat ggttgcggcc gtcgccttgc cgtcgagaac gcccgccatc tactgaacca
      781 gtcccgggta gagcgggaag gcgccggtga gcttggcaac ccgagcacgc agtgcggcga
      841 tgtccgcatc gcccttgagt gcggtcgcga tgatgtcggc gacctcggag aactctgcgg
      901 cggcgaagcc tctcgtcgcc aaagccgggg tgccgatgcg cacgcccgac gtcaccatcg
      961 gcggcctggg gtcgaacggg acggcgttgc ggttgaccgt aatgcccaca tcgtgtagca
     1021 ggtcttgcgc ctgctggccg tcgatcggcg agttgcgcag gtcaacgaga accaagtgca
     1081 cgtcggtgcc gccggtgacg acgttgatgc ctgcctcgat cgcgtccggg gcagtgaggc
     1141 gctccgccag aatgcgcgcg ccctcaagaa cgcgctcctg gcgctccttg aactccgcgc
     1201 ccgcggcgat cttgagcgcg gtcgccttgg cggcgatcac gtgcatgagc ggtccgccct
     1261 gctggccggg gaacaccgcg gagttgagct tcttggccag ttccatgtcg cgagccacga
     1321 tcagtccgga gcgggggccg ccgagcgtct tgtgcacggt cgtcgagacc acatcggagt
     1381 gagggacggg actcgggtgg agatgagcgg ccaccagccc ggcgaagtgc gccatgtcgg
     1441 tccacagctt ggcgcccacc tcgtcggcga tctcgcggaa tgccgcgaag tcgaggtgac
     1501 gggggtaggc ggaccagccc gcgatgatga catcggggcg ctcggcgagc gccgttgcgc
     1561 gcacctgctc catctcgatg cggtgcgact gctcgttgag gccgtacgcg gcgacctcgt
     1621 acagcttgcc ggagaagttg agcttcatgc cgtgcgtcag gtggccgccg tgcgcgagcg
     1681 agaggcccat gatcttctgg ccgggattga tgagcgcgtg aagcactgcc gcgttcgcgg
     1741 tggctcccga gtgaggctgc acgttcgcgt actccgcgcc aaacaggctc ttggcgcgat
     1801 cgatggcgat cgactcggcg acgtccacct cttcgcaacc gccgtagtag cggcggccgg
     1861 ggtagccctc ggcgtacttg ttggtgagca ccgagccttg ggcctgcagg acggcgcggg
     1921 gcacgaagtt ctcgctggcg atcatctcca gcgtctcgcg ctggcgggtc agctcgccct
     1981 ccaggacggc ggcgatctcg gggtccacct gggacagcgg ctggtcgagg agcgaagtct
     2041 cgaggggtgt ctgggaggtc atgtctctcc ttgcggaatc agcagtgcgg gcccgccggg
     2101 cacgcacact ctcggtgtgg gtgacccgga gcccaggcgt acgacccgtg gtcggctgct
     2161 gcgtcgctcc ccggtggtgg cccacctgtt ccgccagtcg cgaccctgcc cagggtagca
     2221 aggcacggca acgccgtgcg cagggatatc gcccgacgcc ggctcgcgcc gcccgacgcc
     2281 gctcaccccc gcgccgccag ccgacgtccc agccaccccc agccgacgtc ccagccaccc
     2341 ccagccgacg tcccaggc
//
LOCUS       DASRKH010000299         2282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_45404, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000299 DASRKH010000000
VERSION     DASRKH010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45404"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..1134
                     /locus_tag="VFC06_04810"
     CDS             133..1134
                     /locus_tag="VFC06_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81247.1"
                     /translation="MEAKPRPLLGSLLGLLLGIVVVALLWQLGVVPPDQLVLFGVLAV
                     TIFLVTSILTQRVVLVRKRFILVTVLCGLMAGVAVAGIPDMIAGGSISDGCFAEGTSS
                     LGSKTPDSTSVADPFDMTRTDSVAWTGESVDVFTDWKSSTGIDVGGFPVEIWSGSSAN
                     EDQKQSNSGVEDVSGRLADIEESTGIALAGIYHVYGNLDANEGGCDMSAYVRLPGEGV
                     FAGTLNLVMWGTLAALIVIILIMAGVVRHSITRAAFPGASASGAAAAAATDPSPTENR
                     AKESAPRAESGRETKPESRRPEPQAKDKPERAWRQEPGRDEPDAPDAPPSGDDKPEV"
     gene            complement(1162..>2282)
                     /locus_tag="VFC06_04815"
     CDS             complement(1162..>2282)
                     /locus_tag="VFC06_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HZL81248.1"
                     /translation="FGLDAIFPSAKIFNTQGQNYLPVDRELMLSYKESESGQIMHTGI
                     NEAGSAAAFQAVGTSYATHGIPLVPVYIFYSMFGFQRTGDQFWAAGDQLTRGFIIGAT
                     AGRTTLTGEGLQHADGHSPLLAGTNRAVVIYDPAFGYEIRHIIRDGIDRMYGPEDGRD
                     KNVMYYLTVYNEPIEQPAEPENVDIEGIIKGIHKFADAPVGDGPQAQILASGVAVPWA
                     LEAQELLQAHWNVHASVWSVTSWNELRREALDCEQETFLHPEREPRTPYLTAKLADAA
                     GPFVATTDFDHLVPDQVRAWIPGDYATLGADGYGFSDTRAAARRYFKIDGPSTAVRVL
                     QQLERRGEVERGTTARAIERYQLHDVTKGTSGNAGGDS"
BASE COUNT          384 a          684 c          783 g          431 t
ORIGIN      
        1 aaagggagta tcggcgcccc gaaaaccgtt cgtgaatggt gaatctcaca gttgaaatcg
       61 gctcttccgt gccccctagc gcacgtgagg cccgatcgct agtgtggttg ctatagcgaa
      121 agcgagaaca tcatggaagc caagcctagg ccgctccttg gatctctcct cgggctgttg
      181 ctcggaatcg tcgttgtcgc gttgttgtgg caactgggcg tagtgccgcc ggatcagctc
      241 gtcctgttcg gcgttctcgc cgtcaccatc ttcttggtga cgagcattct tacccagcga
      301 gtggtgcttg tccgcaagcg cttcatcttg gtgacggtgc tgtgcgggct gatggcgggt
      361 gtcgccgtcg ctggcatccc ggacatgatc gcgggcggat cgatttcgga cgggtgcttt
      421 gcggagggaa cgtcgtcgct gggcagcaag acgccagata gcacgtccgt cgccgatccc
      481 tttgacatga cgcgcaccga ctcggtggcg tggaccggcg agtctgttga cgtgttcact
      541 gattggaaga gttcgactgg catcgacgtc ggcggcttcc cggtggagat ctggagcgga
      601 agctcggcga atgaggatca gaagcagtca aactcaggcg tcgaagacgt ctccggacgt
      661 cttgcagata tcgaggaaag caccggtatc gctctcgccg ggatctacca cgtgtacggc
      721 aatctcgacg ccaatgaggg tggctgcgac atgagcgcct atgtgcggct gccaggcgag
      781 ggggtgttcg ctggcaccct gaacctggtc atgtggggca ccctcgcggc attgatcgtc
      841 atcattctca tcatggcagg cgtggtccga cactcgatta cacgggcggc tttccctggg
      901 gccagcgcct cgggagccgc ggctgcggcc gcgaccgatc cctctccgac ggagaatcgt
      961 gcgaaggaga gtgcgccgcg cgctgagagc gggcgcgaga cgaagccgga atcccggcgc
     1021 ccagagcccc aggccaagga caagccggaa cgcgcgtgga ggcaagagcc tggtcgcgac
     1081 gagcccgacg cacccgacgc accaccgagc ggcgacgaca agcccgaggt ctaggctcac
     1141 tcacccgcga gagatggtcg gttaggagtc gcctccggcg ttgccggacg tgcccttggt
     1201 gacgtcgtgc aattgatacc gttcgatggc gcgcgctgtg gtgccccgtt ccacctcgcc
     1261 gcgacgctcc agttgctgca gcactcgcac cgccgtcgac ggtccgtcga tcttgaagta
     1321 gcggcgcgcc gccgcacggg tgtcggagaa gccgtagcca tcggcgccga gcgtcgcgta
     1381 gtcgcccgga atccacgcgc gtacctggtc gggcacgagg tggtcaaagt cggtggtggc
     1441 gacgaacggg cccgccgcat cggcgagttt ggccgtcagg tacggcgtgc gaggttcgcg
     1501 ctctgggtgc aggaacgtct cctgctcgca gtcgagggcc tcgcgccgca attcattcca
     1561 cgacgtcact gaccagacgg atgcgtgtac gttccagtgt gcctgcagaa gctcctgagc
     1621 ctcgagcgcc cagggaacgg cgacgccgga tgcgaggatc tgagcctgcg ggccgtcgcc
     1681 gacgggagca tccgcgaact tgtggatgcc cttgatgatg ccctcgatgt caacgttctc
     1741 gggctcggcg ggctgctcga tgggctcgtt gtagaccgtc aggtagtaca tgacgttctt
     1801 gtcgcggcca tcctccggcc cgtacatgcg gtcgatgccg tccctgatga tgtgacggat
     1861 ctcgtatccg aacgcagggt catagatgac gacggcgcgg ttggtgcccg ctagcagcgg
     1921 cgagtggccg tcggcgtgct gaaggccctc gcctgtgagc gtggtgcggc cggcggtcgc
     1981 gccgatgatg aagcctcggg tgagttggtc gcccgcagcc cagaactggt cgccggtgcg
     2041 ctggaagccg aacatggagt agaagatgta gaccggcacc agggggatgc cgtgcgtcgc
     2101 gtacgacgtt ccgaccgcct ggaatgctgc ggcggagccg gcctcgttga tgcccgtgtg
     2161 catgatctgc ccggactctg actctttgta gctcagcatc aactcgcggt ccacagggag
     2221 gtagttctgt ccctgcgtgt tgaagatctt ggccgacggg aaaatggcgt caagcccaaa
     2281 gg
//
LOCUS       DASRKH010000300         2234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_23017, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000300 DASRKH010000000
VERSION     DASRKH010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23017"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..782)
                     /locus_tag="VFC06_04820"
     CDS             complement(162..782)
                     /locus_tag="VFC06_04820"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HZL81249.1"
                     /translation="MPTVHLVRHGHVHNPDAVLYGRLPNFHLSDTGRQMADAVAEYLV
                     ETGLPISRVIASPLQRAQETAAPIARAFGLPIETEPRIIEAGNIYEGEPLPSGIKDFL
                     HPRNWWRLRNPWKPSWGEPFTEQADRMWGAIGDAALATPDGDTVMVSHQLPIWVARLS
                     FERRGFLHDPRKRECGLASITSFRFEGGEPVEMTYATPAAHIKAPK"
     gene            complement(787..1050)
                     /locus_tag="VFC06_04825"
     CDS             complement(787..1050)
                     /locus_tag="VFC06_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HZL81250.1"
                     /translation="MNERVVLYTRKGCHLCDNARDLVRGVCADEGVTWREVDIDSDAL
                     LRARLSDLTPVVEVDGVQVGYWRIRPELVRLALATTPGVVGGQ"
     gene            1188..2021
                     /locus_tag="VFC06_04830"
     CDS             1188..2021
                     /locus_tag="VFC06_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HZL81251.1"
                     /translation="MTRQEPAAPTPVAAFFDVDNTIIRGASAFHIARGLKQRGYFRNR
                     DLIRFGWEQLKYLVFGESPEQMTSVRTEALSIIKGWSVAEMAAIGEEVYDEILAMRIF
                     PGTKALIDEHRAQGHEVWFVTASPLEIGRLIARRLKATGALGTIAEHKGGFYTGSLVG
                     DLMHGEAKAVAVRDLAARRNLDLTRSFAYGDSMNDAAMLGAVGHGCAINPDSRLRRHA
                     KRHGWPIQDFRGRNPEGRRSIVRASLTGFVWIVMTILRGIWHTLLAPLRVLKRKRRDS
                     A"
     gene            complement(2133..2231)
                     /locus_tag="VFC06_04835"
     CDS             complement(2133..2231)
                     /locus_tag="VFC06_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HZL81252.1"
                     /translation="MGSVIKKRRKRMSKKKHRKLLRKTRHQRRNKK"
BASE COUNT          378 a          752 c          724 g          380 t
ORIGIN      
        1 ttcgagcggt ccgggccgat cccctgcagc ccactccgtc acggcgccgt cgcccgagac
       61 gtagccgggc gaggtgttgg cgtcgggcgg agcgcatgcg gcgagccaca acaccatggc
      121 aatgaccacc gcgagcagca ccgcgatgcg ggcccgtctg gtcacttggg tgccttgatg
      181 tgcgcggcgg gggtggcgta ggtcatctcc acgggctcgc caccctcgaa gcggaacgac
      241 gtgatcgacg ccaagccgca ctcccgcttg cgagggtcgt gcagaaaacc gcgcctctcg
      301 aacgacaacc gggccaccca gatgggcaac tggtggctca ccatgacggt gtcgccgtcc
      361 ggcgtggcca gagcagcatc gccgatggct ccccacatgc ggtcggcctg ctccgtgaac
      421 ggctctcccc acgacggctt ccacgggttg cgcaggcgcc accagttgcg agggtggagg
      481 aagtccttga ttccagaggg gagcggctcg ccttcgtaga tgttgccagc ctcgatgatg
      541 cgcggctcgg tttcgatcgg cagcccgaag gcgcgggcta tcggcgccgc ggtctcttgc
      601 gcccgctgca agggcgaggc gatcacccga gagataggca gtccggtctc gaccaggtac
      661 tccgcaacgg catcggccat ctggcgccct gtgtcggaca agtgaaagtt cggcaggcgc
      721 ccgtacagca cggcgtccgg gttgtggaca tgcccgtggc ggaccaagtg gacagtcggc
      781 atgaatctat tgtcccccca ccacgccggg ggtcgtcgcc agcgcaagac gcaccagttc
      841 tggacgaatg cgccagtacc cgacctgcac tccgtcgacc tccacgacgg gcgtgaggtc
      901 gcttagtcgc gcacgcagca aggcgtccga gtcgatgtcg acctctcgcc aggtgacacc
      961 ctcgtcggcg cagacccctc gcaccagatc gcgggcgttg tcacacagat ggcagccctt
     1021 gcgggtgtac agcacgacgc gctcattcac accgcaaccc taagccgact tgctggcgcg
     1081 tgaaacaagg ccgctgccag cggacctgtc ccggcgcccc ttcgggtggg cgagcctccg
     1141 caggaacaga ggctcgcgac gatgcttcgc gccgttaggc ttgcttcatg acgcgtcagg
     1201 agccggccgc gccgacccct gtcgccgcgt tctttgatgt ggacaacacg atcattcgcg
     1261 gcgccagcgc tttccacatc gcgcgcggcc tcaaacagcg ggggtacttt cgcaaccgcg
     1321 atctgatccg gttcggctgg gagcaactca agtacctcgt gttcggcgag tctcctgagc
     1381 agatgacctc tgtacgcacc gaggcgctgt cgatcatcaa gggatggtct gtagccgaga
     1441 tggccgcaat cggcgaagag gtgtacgacg aaatcctcgc gatgcgcatc ttccccggca
     1501 ccaaggcgct gatcgacgaa caccgcgccc agggccacga ggtgtggttc gttactgcgt
     1561 cacccctgga gatcgggcgg ctcatcgcgc gtcgtctcaa ggcgaccggc gcgctcggca
     1621 cgatcgccga acacaagggc ggcttctaca cgggcagcct cgtcggcgac ctcatgcatg
     1681 gtgaggcaaa ggccgtcgcg gtgcgcgacc tggccgcccg caggaacctg gacctcaccc
     1741 gcagcttcgc ctacggcgac tccatgaacg acgccgcgat gctcggcgcc gtcggtcacg
     1801 gttgcgctat caaccccgac tcgcgtctgc gccgccacgc caagcgccac gggtggccga
     1861 tccaagactt caggggccgc aacccggagg gacggcgctc gattgtgcgt gcgagcctca
     1921 cgggcttcgt gtggatcgtg atgacgatac tgcgcgggat ctggcacacc ctgctcgccc
     1981 cattgagggt gctcaagcgg aagcggcgcg actcggccta ggctgtcgct gggccaagcg
     2041 gtccaggcac gacaaagccc gcacccctca cgggcgcggg ctcagcgttt cttgcaggct
     2101 tagcgctttt acaggcttag cgttgttggc atctacttct tattgcgacg ctggtggcga
     2161 gtcttacgaa gcaacttgcg gtgcttcttc tttgacatgc gcttgcgacg cttcttgatg
     2221 acggatccca cgga
//
LOCUS       DASRKH010000301         2008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_19419, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000301 DASRKH010000000
VERSION     DASRKH010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19419"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            440..>2008
                     /locus_tag="VFC06_04840"
     CDS             440..>2008
                     /locus_tag="VFC06_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81253.1"
                     /translation="MTVPARTSGSGPEKAPESDATTSVGPTASPSAPVLVEPPSADEL
                     VAGAVATWRGALVEAAGGSTLSDVDLLGDAALDLSAAHPSGIAQLFAGRETRLSNLVR
                     EGGSLATAKRRARDPTLRAESYAQRYGIAPTYLAIGVATWTERTTPDVASDDVAALAA
                     VTRAARSQRAASAYDVEADGGTHTPAGPRTVRAPVLLRPVSLHARGSGESDYELALEP
                     SLEVNPILARALRSRGALLDPGAVARGAFTGTGFDPRPALDRLTSLGAAVLEGFELVE
                     RIVVGTFVHPGQILVDDLDALSGTLDRHEVVAALAGVEEARARLRRPVPAPVRGDRDP
                     DLERGVGDLDAAQQHVLDVLATGEHLFIDAPTGSDVTGTLAAVVADAAASGRTVLYVP
                     GHRRASSALKARLDRLGLGDLLLDVAPEAGWRTAVGRRLLGAMTLEAQAPELARINGV
                     RRDLVEHREQLRAYVEGLHAERGPWGASAYDALQALARLTATRPAPRTTVRLSADVAR
                     VLDADRRTALAADLA"
BASE COUNT          239 a          780 c          708 g          281 t
ORIGIN      
        1 gtaccgagga cccggccgtg aatgatgatc tcgtcgcctg cggaagcatg catcgtcgct
       61 cacctcggtt ccagagtaga cgcgaaccgg ggtgttcggc gaggtcagga ggggcctcag
      121 atcgggccgc ggagttcacc cgtcctcgct ggcctggacg tttgaccagg aaccctccga
      181 ggatccacaa ttgccttgac agtgtgcatc gcctgccccc tgcgcggtgc gcactgttca
      241 ctgtccggtg tcgggcgggc ctccttgagg cccgcccgat cacccgggcc ggctccccgc
      301 cgcagtgcgg gtcgagcccc gtcggatcgc aggtcgaacc ccccgaaaag ccgcgtcgtg
      361 cgcacggact tccgcaccat caccgtgtcg tcggcgtgcc acgcgtccgc gcgcgagcag
      421 tgcggttagc gttcccaacg tgaccgtccc tgcgcgcacg tccggctcgg gacccgagaa
      481 ggctcccgag tccgacgcga ccacctctgt cggcccgacg gcttcgccat cggcgccggt
      541 cctcgtcgag ccaccgtctg cggacgagct cgtggccggc gccgtcgcga cctggcgcgg
      601 tgcgctggtc gaggccgcgg gcggctcgac gctgtccgac gtcgacctcc tcggcgacgc
      661 cgcgctggac ctgtccgcgg cgcacccgtc cggcatcgcg cagctcttcg ctggccgcga
      721 gacccgcctg tccaacctcg tgcgcgaggg cgggtcgctc gcgaccgcga agcgccgggc
      781 gcgcgacccg acgctgcgcg ccgagagcta cgcgcagcgc tacggcatcg ccccgacgta
      841 cctcgcgatc ggcgtcgcga cgtggaccga gcggacgacg ccggacgtcg cgtcggacga
      901 cgtcgcggcc ctcgccgcgg tcacgcgcgc ggctcggagc cagcgcgctg cctcggccta
      961 cgacgtcgag gccgacggcg gcacccacac gccggcagga ccccgcacgg tgcgcgcccc
     1021 ggtgctgctg cgcccggtgt cgctgcatgc ccgggggagc ggtgagagcg actacgagct
     1081 cgctctcgaa ccgtcgctcg aggtcaaccc gatccttgcc cgcgcgctgc gcagccgtgg
     1141 cgcgctcctg gacccgggcg cggtggcccg gggtgcgttc acgggcaccg ggttcgaccc
     1201 gcggcccgca ctcgaccggc tcacgtccct cggcgccgcc gtgctcgagg gcttcgagct
     1261 cgtcgagcgc atcgtcgtgg ggacgttcgt gcaccccggt cagatcctcg tcgacgacct
     1321 cgacgcgctg tccgggaccc tcgaccggca tgaggtcgtc gcggcgctcg caggcgtcga
     1381 ggaggcacgc gcacgtctgc gacgccccgt tcccgcgccc gtgcgcggcg accgggaccc
     1441 tgatctcgaa cgtggtgtcg gggacctcga cgccgcgcag cagcacgtcc tcgacgtcct
     1501 cgcgacgggc gagcacctgt tcatcgacgc gcccaccggg agtgacgtca ccggcacgct
     1561 cgcggccgtc gtggccgatg cagcggcgtc gggacgcacc gtcctgtacg tcccggggca
     1621 ccggcgggcc tcctcggccc tcaaggcccg gctcgaccgg ctcggtctcg gcgaccttct
     1681 cctcgatgtg gctcccgagg ccggctggcg caccgccgtc ggtcgtcggc tgctcggcgc
     1741 gatgacgctc gaggcgcagg cacccgagct cgcgcgcatc aacggcgtgc gccgggacct
     1801 cgtcgagcac cgtgagcagc tccgtgccta tgtcgagggg ttgcacgccg agcgcgggcc
     1861 gtggggtgcc tcggcgtacg acgcgctgca ggcgctcgcg cggctcaccg cgacccgtcc
     1921 cgcaccgcgc accacagtgc gtctgagcgc cgatgtcgcg cgcgtgctcg acgccgaccg
     1981 gcgcaccgcg ctcgcggccg acctggcc
//
LOCUS       DASRKH010000302         3340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_18494, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000302 DASRKH010000000
VERSION     DASRKH010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18494"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VFC06_04845"
     CDS             complement(<1..367)
                     /locus_tag="VFC06_04845"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HZL81254.1"
                     /translation="MGASIGIGLTSEGWHVTISDADDEAQLLARSIGAGLELGTEQPE
                     LVIVATPPGVTAQVVVDALNRWPDAVVVDVASVKAPIADVAERTGQAHRYVGSHPIAG
                     REISGGMAAQGDLFRARPWV"
     gene            complement(409..756)
                     /gene="aroH"
                     /locus_tag="VFC06_04850"
     CDS             complement(409..756)
                     /gene="aroH"
                     /locus_tag="VFC06_04850"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HZL81255.1"
                     /translation="MRGATTLERDEREHLHERTRELVEGMLRDNDITAADVISVFFTC
                     TPDIVSDFPAAAARKLGFGAVPLMCAQEMAVPGALPLVVRVMMHVEMDRPRDAVSHVY
                     LHDAVSLRRDLAQ"
     gene            complement(820..1569)
                     /locus_tag="VFC06_04855"
     CDS             complement(820..1569)
                     /locus_tag="VFC06_04855"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HZL81256.1"
                     /translation="MGDLEIYRPEGVRLQKVLAQAGVGSRRKCEDLIEAGKVMVNGEV
                     VDQLGVRINPDECVIKVDGKRVSVDTHLITVVLNKPKGVVSTMSDPEGRPSLEQYVKE
                     YDERLFHVGRLDADTTGVLLLTNDGDLANKLAHPTHGVSKVYVAKVQGRVQKSISNTL
                     RGGLELDDGPVRAVECRIIDATENHSLVEVVMHEGRNRIVRRMFDAVGHPVIDLVRTK
                     FGPIGLGTLQAGDQRRLSNTEVGLLQQEAGL"
     gene            complement(1569..2108)
                     /gene="scpB"
                     /locus_tag="VFC06_04860"
     CDS             complement(1569..2108)
                     /gene="scpB"
                     /locus_tag="VFC06_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005274525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HZL81257.1"
                     /translation="MSVPVRPALEAILMVVDEPATPVDLATALDVPEPQVVDALHALR
                     DEYANPDNPRGFELREVGGGWRIYSSPLFASAVGAFVVEGQSARLSQAALETLAVVAY
                     RQPATRGQVSQIRGVNVDSVMKTLLNRGLVTEVGEVGGAVQYGTTTEFLERIGLGSLD
                     DLPALAPYLPDLDDVDEAN"
     gene            complement(2105..2920)
                     /locus_tag="VFC06_04865"
     CDS             complement(2105..2920)
                     /locus_tag="VFC06_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HZL81258.1"
                     /translation="MTAAVEGARNAFEVHLPNFEGPFDLLLSLISKHEMEITDVALAI
                     VTDEFLVAVRARDGDWDLSQASEFLVVAATLLDLKAARLLPQGEVDDAEDLALLEARD
                     LLFARLLQYRAFKEVAGAVEKLMDAPRRMPRDVTLEQHFAMLLPELVWRATSDDLARL
                     AAHALQPKPQDLGVSLTHLHNPKVSVREQAAIISSRLQRAGAMTFRSLVADADHPAMV
                     VARFLALLELFRESAVAFEQVRSLGELTIRWTGSREDIQVSGEFDGVEEEPTP"
     gene            complement(2922..>3340)
                     /locus_tag="VFC06_04870"
     CDS             complement(2922..>3340)
                     /locus_tag="VFC06_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HZL81259.1"
                     /translation="YDLILIDCQPSLGLLTVNALVAAHGVIIPLACEYFALRGVALLV
                     DTVSKVQDRLNPKLTMDAIVPTMFDPRTLHAQEVVDRVRDAFGDRVTETMIGRTVKFP
                     DSSVASESILTFAPKHVGAESYRQLARELVTRGVAA"
BASE COUNT          573 a         1117 c         1093 g          557 t
ORIGIN      
        1 gcacccaggg cctggccctg aagaggtccc cttgcgcggc catcccgccc gagatttcgc
       61 gcccagctat cgggtgggag cccacgtagc ggtgagcctg acccgtgcgt tcggccacgt
      121 cggcgatggg cgccttcacg ctcgcgacgt cgaccaccac cgcatcgggc cacctgttga
      181 gagcgtcgac cacgacctga gcggtcacgc cggggggcgt tgccacaatc acgagctcgg
      241 gctgctcggt tccgagttcc agacccgcgc cgatgctcct tgccaacaac tgagcctcgt
      301 cgtccgcatc ggagatggtg acatgccatc cctcggaggt gaggccaatg cctatcgacg
      361 cccccatgag acctgcgccg atgatgtgag tagtgggcgt gggtgccatt actgggcgag
      421 atcccggcgc aaagacacgg catcgtgcag gtacacgtgg gacaccgcgt ctcgcggtcg
      481 gtccatctcg acgtgcatca tgacgcgcac cacgagcggc aaggcgccgg gcactgccat
      541 ctcttgagcg cacatgagcg gcaccgcccc gaagcccagc ttgcgggcgg ccgcggcggg
      601 aaagtcgctg acgatgtccg gtgtgcaggt gaagaacacg gagatgacgt cggcggccgt
      661 gatgtcgttg tcgcggagca ttccctcgac gagttcgcgg gtgcgctcgt ggaggtgctc
      721 tcgctcatcg cgttcgagcg tagttgcgcc cctcatcgcc cgtacagcca tactgcaatg
      781 gtaccgggcc acgggccccg cgggtggccg agcggagcgc tacaggcccg cctcttgctg
      841 cagcaacccg acttcggtgt tgctgaggcg acgctggtcg ccggcctgaa gggtgccgag
      901 gccgatcggc ccaaacttgg tgcgcaccag atcgatgacc gggtgcccca ctgcgtcgaa
      961 catgcggcgc acgatgcggt tacggccctc gtgcatgacc acctccacca gggagtggtt
     1021 ttcggtggcg tcgatgatgc ggcattcgac ggcccgcacg ggaccatcgt ccaactcgag
     1081 tcctccgcgc agcgtgttgc tgatgctctt ctggactcgc ccctgcacct tcgcgacgta
     1141 caccttggac acgccgtggg tggggtgggc gagcttgttg gcgaggtcgc cgtcattggt
     1201 gaggagcaac acgcccgtgg tgtccgcgtc gagccgaccc acatggaaca gtcgctcgtc
     1261 gtactccttg acgtattgct cgagtgacgg ccgaccctcg gggtcgctca tggtgctcac
     1321 cacgcccttg ggcttgttca gcaccaccgt gatcaggtgg gtatccacgg agacccgctt
     1381 gccgtcgacc ttgatcacac actcgtcggg attaatgcgc acgcccagtt ggtcaacgac
     1441 ctcgccgttg accatcacct tgcctgcctc gatgaggtcc tcgcacttgc ggcgcgaccc
     1501 cactccggcc tgcgcgagca ccttctgaag gcgcacgccc tcggggcggt aaatctccag
     1561 gtcgcccatc aattcgcctc atccacgtcg tcgagatccg gcagatacgg ggccaacgcc
     1621 ggcaaatcat ccaaggaacc caggccgatg cgctcaagga actcggtggt ggtcccgtat
     1681 tggacggcgc cgcccacttc tccgacctcg gtgacgagac cgcggttcag caacgtcttc
     1741 atcactgaat ctacgttaac gcctcggatc tgcgagacct gaccgcgggt ggctggctgg
     1801 cggtaggcca ccacggcgag cgtctcgagc gcggcctgag agagcctggc cgactggccc
     1861 tccaccacga aagccccgac agcgctggcg aacaacgggg acgagtagat gcgccagccg
     1921 ccgcccacct cgcgcagctc gaagccgcgg gggttgtctg ggttcgcgta ttcatcgcgc
     1981 agcgcgtgca gggcgtcgac cacttgcggc tcgggaacgt ccagggccgt cgcgaggtcc
     2041 actggcgtgg cgggctcgtc gacaaccatg agaatcgcct cgagggcggg gcgcaccgga
     2101 acactcatgg ggtgggttcc tcctcgacgc cgtcgaattc tccactcacc tgaatgtctt
     2161 cgcgggaacc ggtccagcga atagtgagct cgccgagcga gcgtacctgc tcaaaagcga
     2221 ctgccgattc gcggaagagc tccagcagtg cgagaaaacg cgccacgacc atggcggggt
     2281 ggtctgcgtc ggcgacgagc gaacggaacg tcatcgcccc ggcacgctgg agccgactgg
     2341 agatgatggc ggcctgctcg cgcacgctga ccttcggatt gtgcaggtgg gtgagggaga
     2401 cgccgaggtc ctgaggcttt ggctgcagcg cgtgcgccgc caacctggcc aggtcgtccg
     2461 acgtcgcccg ccacaccagc tccggcagca gcatggcaaa gtgctgctcg agcgtcacat
     2521 cgcgcggcat tctgcggggc gcgtccatga gcttctccac cgcacccgcg acctccttga
     2581 acgcccggta ctgaagcaga cgcgcgaaca gcaagtcccg cgcctcgagg agcgcgaggt
     2641 cttccgcgtc gtccacttcg ccttgcggca ggagccgggc ggccttgagg tcgagcagcg
     2701 ttgccgcaac caccaggaac tctgaggcct gggacaggtc ccagtcgccg tcacgcgcac
     2761 ggacggccac caggaactcg tcggtcacga tcgccaacgc gacgtccgtg atctccatct
     2821 cgtgcttgga gatcaggctc aacagcagat cgaaaggccc ttcgaaattg ggaaggtgca
     2881 cctcgaaggc gttgcgtgcc ccttcaacgg cggcggtcac gtcaggctgc caccccgcgc
     2941 gtcaccagtt cgcgggcgag ctgacggtag gactccgcgc cgacgtgctt gggcgcgaac
     3001 gtgagaatcg actcggaggc gaccgaggaa tcggggaact tcaccgtgcg gccgatcatc
     3061 gtctcggtca cgcggtcgcc gaacgcgtcg cgcacgcgat ccaccacctc ttgagcgtgc
     3121 aaggtgcggg ggtcgaacat cgtcggcacg atggcgtcca tcgtcagctt ggggttgagc
     3181 cggtcctgca ccttgctcac ggtgtcgacc agcagggcca ccccacgcag cgcgaagtac
     3241 tcgcaagcga gaggaatgat cacgccgtgc gcggccacca gggcattcac ggttaacagg
     3301 ccgagcgacg gctggcagtc gatcaggatc aggtcgtagt
//
LOCUS       DASRKH010000303         2105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_63071, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000303 DASRKH010000000
VERSION     DASRKH010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63071"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..947)
                     /locus_tag="VFC06_04875"
     CDS             complement(24..947)
                     /locus_tag="VFC06_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009358231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZL81260.1"
                     /translation="MLDLTRLAMLREFAVRGTLAATAEAMGYTPSAISQQLAVLEREA
                     GTPLTVRSGRRLRLTPAGERLVVHANAVLATLEEAQAELRLHRESVRGTVKLAVFQSA
                     ALALVPLALRALGTAHPDLRVLVTQSEPGAALADTWARDFDLVVAEEYPHHSAPHYPD
                     VDRRPLVTDEIRLCVGGPWEDATGLADVSGATWVMEPRGTATRHFAEQACRTAGFEPD
                     VRFETADLQAHVRYIESGLAVGLLPGLMWGRGDMAASAHSLTPAAHRTVFTAARRTSR
                     HDSAIGAVRDALEHAAQELGVMAGTAPSVRG"
     gene            1050..2099
                     /locus_tag="VFC06_04880"
     CDS             1050..2099
                     /locus_tag="VFC06_04880"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HZL81261.1"
                     /translation="MRATPEQSPGVVGQQHGTEDVTALVQHWLIASRAYPVKGSAALL
                     AGLLREDGGLAFLTQFVDGVIRPEDTIVAARNFRSMAARDSHFLPAYQRVLLRLGSWA
                     SRVLPRLVVAVARRTVRLMVSHLVVDATPKRLGPALGRLSAEGHRLNVNLLGEAVLGR
                     AEAARRLDRTQALLSRPDVDYVSLKVSSAVAPHNPWAFEENVAHIVDTLRPLFRAALD
                     ADGAFISLDMEEYRDLDLTMAVFTALLDEDEFAALTAGIVLQAYLPDSMGAMVHLQEW
                     ASARVARGGAPIKVRLVKGANLSMERVDAETHGWPLATWHSKAESDAQYKRLVDYALV
                     PERTRAVRVRSGTSA"
BASE COUNT          339 a          692 c          739 g          335 t
ORIGIN      
        1 gcggtgtggg acaacgcgtt ccattagccg cgaacggacg gggcggttcc ggccatcacg
       61 ccaagttcct gtgcggcatg ctcgagcgcg tcgcggacgg ctccgatagc cgagtcgtgc
      121 cgcgacgtgc gccgggccgc cgtaaagacc gtgcggtgcg cggctggtgt gagcgagtga
      181 gccgacgcgg ccatgtctcc tcgcccccac atgaggccgg gcagtagtcc gacggcgaga
      241 cccgactcga tgtagcgcac gtgagcctgc aggtccgcag tctcgaagcg cacgtccggc
      301 tcaaagcccg cggtacggca cgcctgctcc gcaaagtgac gcgtcgccgt gccacgtggc
      361 tccatcaccc acgtagcacc ggacacgtcg gctagtcccg tcgcgtcctc ccatggtccg
      421 cccacgcaca acctgatctc gtcggtgacg agcgggcggc ggtccacgtc ggggtagtgg
      481 ggcgccgagt ggtgcgggta ctcctcggcg accacgaggt cgaagtccct cgcccatgtg
      541 tccgcgagcg cggcgcccgg ctcggattga gtcacgagca cccggaggtc ggggtgggcg
      601 gtgccgagcg cgcgcaacgc cagaggcacg agcgccagcg cggctgactg gaagacggct
      661 agcttcacgg tgccccgcac ggactcccga tgcagtcgga gctcggcctg cgcctcctca
      721 agggtggcga gaacagcgtt cgcgtgcacc acgagccgtt cgccggccgg ggtgagccgc
      781 aatcgcctgc cggaccgcac ggtgaggggg gtgccagcct cgcgttcgag caccgcgagc
      841 tgttgtgaga tcgcggacgg ggtgtacccc atggcctcgg cggtggccgc cagagtgccg
      901 cgcacggcga attcgcggag catcgcaagg cgggtcaggt ccaacatgtc gttagttaag
      961 cacacctaat gtaattgcgt gagaaacatg aactggtgct gactggcttc gacgacggac
     1021 actgtgcgaa acaccatgga ggagagacga tgcgagcaac gcccgagcaa tcaccagggg
     1081 tggtcggaca gcaacacggg accgaggacg taacggccct cgtccagcac tggttgatcg
     1141 cgtctcgggc gtacccggtc aagggttccg cagcgctgct cgcggggctc ctgcgagagg
     1201 acggcgggct cgccttcctg acacagttcg tggacggggt gatccgtccc gaagacacca
     1261 tcgtcgccgc cagaaacttc cgttccatgg cagctcgcga ctctcacttt cttccggcct
     1321 accagcgggt cctgctgcgg ctggggtcgt gggcctcgcg tgttctgccc cgactcgttg
     1381 tcgcggtagc tcggcgcacc gtgcggctga tggtgtcgca cctggtggtg gatgccactc
     1441 ccaagcgact cggtcccgcg ctcgggaggc tgagtgccga aggacaccgc ctcaacgtga
     1501 acttgctggg cgaggccgtc ttgggtcgcg cggaggccgc gcgacgactg gatcgcacgc
     1561 aggcgttgct gagccgaccg gatgtcgact acgtgtcgct caaggtgtcg tccgcggtcg
     1621 ccccgcacaa cccttgggcg ttcgaagaaa acgtcgccca catcgtcgat accttgcggc
     1681 ccctcttcag ggcggccctc gacgcggacg gcgcgttcat cagcctcgac atggaggagt
     1741 accgcgatct cgacctgacg atggctgtat tcaccgcgct gctcgacgag gatgaattcg
     1801 cggccctcac cgcgggcatc gtgctccagg cctacctgcc cgactccatg ggggcgatgg
     1861 tccatctcca ggagtgggcc tccgcgcggg tggcgcgggg gggagccccg atcaaggtgc
     1921 ggttagttaa gggcgccaac ctgtcgatgg agcgggtgga cgccgaaacg cacggatggc
     1981 cgcttgccac gtggcactcg aaggcggagt cggacgcgca gtacaagcgc ctggtggact
     2041 acgcgctggt tccggagcgc acgcgtgcag tgcgcgtgcg ctccggaacc agcgcgtagt
     2101 ccacc
//
LOCUS       DASRKH010000304         2552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_24988, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000304 DASRKH010000000
VERSION     DASRKH010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24988"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="VFC06_04885"
     CDS             <1..669
                     /locus_tag="VFC06_04885"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZL81262.1"
                     /translation="RQRTAFDAADTVTWLSANATTFGIRAINYNDVQLTFVAPADRNE
                     DGDKYIVETLRSGTSIENTLLVSVHGTAGTGVLEGGAGGAWVLPVAVSCVSSEYGMRN
                     HPTLHRWILHAGMDFPVGSGTNALAVTGGTVSRVFYDSGGGNILEIDYGGGITMRYLH
                     LSKYLVAEGQAVSSGQVVALTGNTGTRTTGAHLHFEVYRDGRSTNPRDFFANLGVTVP
                     LACW"
     gene            751..1935
                     /locus_tag="VFC06_04890"
     CDS             751..1935
                     /locus_tag="VFC06_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81263.1"
                     /translation="MVDVDVFETMWALGVPSSDMTDLRKVEPFRISTVGERLMLRKAM
                     EDTFDVPSDGFVENPWGFPPMPLPVRRSTTSDGLIIAPSSISLDGLGHPIFWIDPSLT
                     RRTDEEREHPERWGVRMFYLIMAIGGYDPESLKWINIPRSQGVDYTKEDWIAYRQGVS
                     SVLSTVRPLDESDLAEPMASINVMVDWALATCVELQAKVFTDYRAELDAAYTTAVAST
                     QGRSEWDVWDAKMKDATARIAATVESGAVPSSQVPEIYGLVDDLERVVKHTERCASML
                     SIPVVTAGSAGDSAAAAATALNAAKSDVDVSVDSLKQEAHRLFELSSTSSPREFVRLH
                     ESATSLYMRSWRAMMVAARNLVRASEGQSAYQSWEALELVEDPHSQAWPPLMDLLAGL
                     GR"
     gene            1946..2518
                     /locus_tag="VFC06_04895"
     CDS             1946..2518
                     /locus_tag="VFC06_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81264.1"
                     /translation="MFIAYALALLTSIGWGAYALRWYVRERLSQRDARDSDDRQWFEI
                     IQESGKKAGPYTASSFDGMRAISQLLNGDISLAPTKALPKVSFVWVRSADDKAQLTRL
                     YVSASESLGESGLPALRAYARSIGARLGTPTVLRLPTENVIMGRRRHIALGQMRDKVD
                     EIGVVSDTIASIANAVARDESVAVMITVEG"
BASE COUNT          452 a          820 c          818 g          462 t
ORIGIN      
        1 agacagcgaa cggctttcga cgccgcggac actgtcacgt ggctctcagc gaacgcgacc
       61 acgttcggca tccgggcgat caactacaac gacgtccagt tgacgttcgt cgcgcctgcc
      121 gaccgaaacg aggacggcga caagtacata gtcgaaacgc tacgttcagg gacgtcgatc
      181 gagaacacgt tgctggtgtc cgtgcatggg actgctggca cgggcgtgct cgaaggcggc
      241 gccggtggcg cttgggtgct cccggtcgca gtgtcgtgcg tctcgagcga gtacgggatg
      301 cgtaatcacc cgacgcttca ccgctggatt cttcacgccg ggatggactt cccagtcggc
      361 tccgggacga atgcgttggc ggtcactggc ggcaccgtgt cgcgcgtctt ctatgactcc
      421 ggtggcggca acatcttgga gatcgactac ggcggcggca tcaccatgcg atacctccac
      481 ctgtcgaagt acctcgtcgc cgaagggcag gccgtctcgt ccggacaagt agtcgcgctg
      541 acgggcaaca ctggcacgcg aacgaccggt gctcacctcc actttgaggt ctaccgtgac
      601 ggccgcagca cgaacccgag ggactttttc gcgaacctcg gagtgaccgt ccctctcgcc
      661 tgctggtagg gcgacatttc gcggtagttc tgggcgaaat tggcacccca atagcgccct
      721 accatggggc cagatcctag gagggttcct gtggtagacg tagacgtttt tgagaccatg
      781 tgggcgttgg gcgttccctc gtcagacatg accgacctga ggaaggtcga accgttccgc
      841 atctcgacag tgggcgagcg tctcatgctg cgcaaggcga tggaggacac cttcgacgtg
      901 ccctctgacg ggttcgtgga gaacccgtgg ggcttcccgc cgatgccgct gcccgtgcga
      961 cggtccacga cctctgacgg gctcatcatc gcgccgtcat ccatctcgct ggacggcctc
     1021 gggcacccga tcttctggat cgacccttcg ctgacacgtc gcaccgacga agagcgggag
     1081 cacccggagc gttggggtgt ccggatgttc tacctgatca tggcaattgg cgggtacgac
     1141 cctgaaagcc tcaagtggat caacatcccg cgctcacagg gcgtcgacta cacgaaggaa
     1201 gactggatcg cgtaccgcca gggcgtttcg tctgtgctca gcacagtgcg cccactagac
     1261 gagagcgacc ttgctgagcc gatggcgagc atcaacgtga tggtcgattg ggccctggcc
     1321 acctgcgtgg agttgcaggc gaaggtcttc acggactacc gcgccgaact cgatgctgcc
     1381 tacacgactg ctgtcgcatc aacgcagggc cgctccgagt gggacgtgtg ggacgcgaag
     1441 atgaaggacg cgaccgcccg catcgccgcc acggtggagt ccggtgctgt gccctcatcg
     1501 caggtccccg agatttacgg gctcgttgac gacctcgaac gcgtagtgaa gcacaccgaa
     1561 cgctgcgcgt ccatgctttc cattcccgtg gtcaccgcgg gctccgccgg ggacagtgcg
     1621 gctgcggccg cgacagcgct caatgcggcc aagtccgatg tggacgtgag cgtcgactcg
     1681 ctgaagcaag aggcgcaccg actcttcgaa ctgtcgtcga cgtcatcgcc gcgagagttc
     1741 gtccgtcttc acgagtccgc cacgtcgctg tatatgcgct cctggcgggc gatgatggtg
     1801 gccgcccgca acctggttcg cgcatcggaa gggcagagcg cttaccagtc atgggaggcg
     1861 ctcgaactcg tcgaggaccc ccactcgcag gcatggcccc cgctgatgga tctgctcgcg
     1921 ggcctgggcc ggtaggcggc cctccatgtt cattgcatac gcactggcgt tactgacctc
     1981 tatcgggtgg ggcgcgtacg ccttgcgctg gtacgtgcga gagcgcctca gccaacgcga
     2041 cgcccgtgac tcggacgacc gtcaatggtt cgagatcatt caggagtccg gcaagaaggc
     2101 cggcccgtac accgcgtcat ccttcgacgg catgcgtgcg atctcccaac tgctcaacgg
     2161 cgacatctcg cttgcgccga ccaaggcgct gccgaaggtg tcgttcgtgt gggtccgctc
     2221 cgccgacgac aaggcgcagt tgacgcgcct gtacgtgagc gcgagcgagt cactgggcga
     2281 atccgggctc cccgcgctgc gtgcgtacgc ccgctcgatc ggcgcacgac ttggcacgcc
     2341 cacagtgctg cgactcccga ccgagaacgt catcatgggg cgacgacgcc acattgcact
     2401 cggtcagatg cgggacaagg tcgacgaaat cggggtcgtc tccgacacga tcgcttccat
     2461 cgccaacgca gtggcacgcg acgaatcggt cgccgtcatg atcaccgtcg aaggatgacg
     2521 cggtgtacgg gccggccttc ttgccggact cc
//
LOCUS       DASRKH010000305         2051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_13961, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000305 DASRKH010000000
VERSION     DASRKH010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13961"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VFC06_04900"
     CDS             complement(<1..324)
                     /locus_tag="VFC06_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015771401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase domain-containing
                     protein"
                     /protein_id="HZL81265.1"
                     /translation="MTERIVLAYSGGLDTSVAIGWIADATGAEVVAVAVDVGQGGEDL
                     EVIRQRALDCGAVEAYVADARDEFAEEYCMPALKANALYHDKYPLVSALSRPVIVKHI
                     VQAARE"
     gene            complement(343..834)
                     /locus_tag="VFC06_04905"
     CDS             complement(343..834)
                     /locus_tag="VFC06_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882858.1"
                     /note="regulates arginine biosynthesis when complexed with
                     arginine by binding at site that overlap the promotors of
                     the arginine biosynthesis genes; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HZL81266.1"
                     /translation="MSPTIPGTKSARQALIARLIESEEIRSQGDLAMRLTVQGISVTQ
                     ATLSRDLLDIGALKVRGASGFMVYTTQASLEASGDTGGRLNRLCAELLFSADGSANIA
                     ILRTPPGAAQYLASGIDGHDDPDVVGTVAGDDTVFVISRDALGGPTLARTFLDRASGV
                     AHD"
     gene            complement(831..1778)
                     /gene="argF"
                     /locus_tag="VFC06_04910"
     CDS             complement(831..1778)
                     /gene="argF"
                     /locus_tag="VFC06_04910"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HZL81267.1"
                     /translation="MVRHFLRDDDLTAAEQREVLELALAFRKNRYLHRPFDGPQAVAV
                     LFDKPSTRTRVSFSAGVVELGGYPLVLDAGSSQMGRGESVADTARVLGRQVSAIVWRT
                     FGQDRVEEMAQYAGVPVVNALTDDFHPCQILADLAAIADARGGVDALAGLTLAYVGDG
                     ANNMAHSYLLGGALAGMHVRVGAPEDYFPSEAIVLDAKRLAAEHGGSVTVTTNHDDAV
                     RGADIIATDTWVSMGDEAESGERTGHFDSYQVNARSLEIAAPGAQVLHCLPAYRGKEI
                     TADVLDGPQSIVWLEAEYRLHAQKALLTWLAAHAREDAS"
     gene            complement(1778..>2051)
                     /gene="argD"
                     /locus_tag="VFC06_04915"
     CDS             complement(1778..>2051)
                     /gene="argD"
                     /locus_tag="VFC06_04915"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the formation of N-acetyl-l-glutamate
                     5-semialdehyde from 2-oxoglutarate and
                     N(2)-acetyl-L-ornithine; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HZL81268.1"
                     /translation="MGEWIKATVGALPGVVEVRGEGLLLGIRLEAEISAAVAAAAIED
                     GFIVNPPSPDTIRLAPSLTLTQAEAEPFVQWLADYLAAHAADTKEA"
BASE COUNT          321 a          686 c          715 g          329 t
ORIGIN      
        1 ttcgcgcgcc gcctgcacga tgtgcttgac gatgacgggt cggctgagcg ccgagacgag
       61 cgggtacttg tcgtggtaga gcgcattcgc cttgagcgcg ggcatgcagt actcctccgc
      121 aaactcgtcg cgagcgtcgg ccacatacgc ctccacggcg ccgcagtcga gcgcgcgctg
      181 gcgaatcacc tcaaggtctt cgcctccctg gccgacatcc accgcaacgg ccaccacctc
      241 tgcgccggtc gcgtctgcga tccagccgat ggcgacggag gtgtcaaggc cgcctgagta
      301 tgcgaggacg atgcgttcgg tcatgtgtgt ttctcctgag atttagtcgt gggccacgcc
      361 ggaagcgcgg tcgaggaacg ttcgggcaag ggtcgggccg ccgagggcgt ccctggaaat
      421 cacgaagacg gtgtcgtctc ccgcgacggt tccgacgacg tcggggtcgt catggccgtc
      481 aatgccggag gcgaggtatt gcgccgcgcc gggaggggta cgcaggatgg cgatgttggc
      541 cgagccgtcg gcagagaaca ggagctccgc gcacagccgg ttgaggcgtc cgccggtgtc
      601 gcccgatgcc tcgagcgagg cctgcgtcgt gtagaccatg aagcccgatg ctccccgcac
      661 cttcagcgcg ccgatgtcga gcaggtcgcg cgacagggtc gcctgcgtga ccgagatgcc
      721 ctgcacggtg agccgcatcg cgaggtcgcc ctgcgagcgg atctcttccg actcgatcag
      781 gcgcgcgatg agcgcctgac gggccgactt ggtgccgggg atcgtggggc tcatgacgcg
      841 tcctcacgcg catgcgcggc cagccacgtc agcagcgcct tctgggcgtg cagacggtat
      901 tcggcctcga gccacacgat gctttgcggg ccgtcaagaa cgtcggccgt gatctccttg
      961 ccgcggtaag cgggcagaca gtgcagaacc tgtgctcctg gcgccgcgat ctcgaggcta
     1021 cgagcgttga cctggtacga gtcgaagtgg ccggtgcgct cacccgattc ggcctcgtcg
     1081 cccatcgaca cccacgtgtc ggtggcgatg atgtccgcac cgcgtaccgc gtcgtcgtga
     1141 ttcgtggtga ccgtcacgga gccgccgtgc tccgccgcga gccgcttggc gtccaggacg
     1201 atcgcctcgg acggaaagta gtcctctggc gctccgacgc gcacgtgcat tcccgcgagc
     1261 gcgccgccaa gaaggtagct gtgcgccatg ttgttggcgc cgtcgcccac gtacgcgagc
     1321 gtcagacccg cgagggcgtc cactccccca cgcgcgtcgg cgatggctgc gaggtccgcg
     1381 aggatctggc acgggtgaaa gtcgtcggtg agcgcgttga caacgggcac gcccgcgtac
     1441 tgggccatct cctcgacgcg gtcctggccg aaggtgcgcc acacgatcgc gctgacctga
     1501 cgtcccagca cccgcgcggt gtcggcgacg gactcgccgc gacccatctg cgaggagccg
     1561 gcgtcgagca ccagcgggta tccgcccagc tcgacgacgc ccgcagagaa agacacacgc
     1621 gtgcgtgtgg acggcttgtc gaacagcacg gccaccgcct ggggaccatc gaagggccga
     1681 tgcaggtagc ggttcttgcg gaacgcgagc gcaagctcga gtacctcgcg ctgctcggcg
     1741 gcggtgaggt cgtcgtccct caaaaagtgg cgcaccatca cgcctccttc gtgtcggcgg
     1801 cgtgggccgc gagatagtcg gcaagccact ggacaaaggg ctccgcctcc gcctgagtga
     1861 gcgtgagtga aggggccagc cggattgtgt cggggctggg cgggttgacg atgaagccgt
     1921 cctcgatagc tgcagcggcg accgcggcgg agatctccgc ctcgagcctg atgcccagga
     1981 ggaggccctc tccgcgcacc tcgactacgc cgggcagcgc gcccaccgtg gccttgatcc
     2041 actcccccat g
//
LOCUS       DASRKH010000306         2109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_49307, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000306 DASRKH010000000
VERSION     DASRKH010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49307"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..983)
                     /gene="rsmI"
                     /locus_tag="VFC06_04920"
     CDS             complement(144..983)
                     /gene="rsmI"
                     /locus_tag="VFC06_04920"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HZL81269.1"
                     /translation="MPGRLILAATPIGNVRDGSARLWEALATADVVAAEDTRVTKELA
                     MRAQIHIGGELVALHEHNEGHVAQRLVDRVLAGDTVVVVSDAGMPLVSDPGYRLLTVA
                     IVADAPIEVFPGPSAALAALALSGLPTDRFAFEGFPPRKEGEKAAALAALAGEQRTLI
                     FFESPHRVAATLAAMAEAFGGDRQASVSREITKRYEQTRRGPLADLAAWAAADQLRGE
                     VAVVVAGRSAEAPTVERLVAEALTRIGEGERAKAVVADLAATFGVPQRDLYAAVVAAR
                     SSG"
     gene            1053..>2109
                     /locus_tag="VFC06_04925"
     CDS             1053..>2109
                     /locus_tag="VFC06_04925"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HZL81270.1"
                     /translation="MILTLWRWRATVMATGVTLLAAVLRFADLAHPRALVFDEVYYVR
                     GAYSLLTMGYEGNWGDDNGHFAQGDFSDLETEGDYVVHPMVGKLLIAGGMKLFGNGPF
                     GWRFAGAFLGTVTVLMVALLMRRIMKSTMWGGVAGLLLAIDGLHIVMSRTAILDVFLT
                     FFVVAGFALLWLDRWRAQRIYDAHPADTVGGFGPQVGIRWWRLLALVTFGLATGVKWS
                     GLYFAAAFMVLFVVLDAGDRKAAGYRHWLSAAALRSALPGLLATLVVLPTVYVAQWAN
                     WFATDGSYRRHWAEDHPGEGVTWLPDPLRSLWSYHVSMYNFHKDLDSPHPWESHPAGW
                     IIQFRPTSFFFERIEDAR"
BASE COUNT          291 a          728 c          715 g          375 t
ORIGIN      
        1 gacgatcccg gaactgcgtg ctgcggtcgc agccaacccg tcggcgtatc ccgagttgct
       61 cgagtggatg gggcagttgg gcgaagccgc ggtcgacgcg gcgatcgccg cgcgcgccaa
      121 ggactgatag cgaagagcgg ccgtcagccg ctgctgcgcg ccgcgaccac ggctgcgtag
      181 aggtcccgct gcggcacgcc gaacgttgcc gccaggtcgg ccaccaccgc cttggcgcgc
      241 tcgccttcgc cgatgcgcgt cagggcttcg gcgacgaggc gctcgactgt tggcgcctcc
      301 gctgagcgtc cggccaccac cacggcgacc tcgccgcgta gctgatccgc cgccgcccaa
      361 gcggccaaat cagcaagcgg tcctctgcgg gtctgttcgt atcgcttcgt gatctcgcgc
      421 gacacggacg cctgacggtc gccgccgaag gcctcggcca tcgcagcgag ggtcgcggcg
      481 acccgatgcg gcgactcgaa gaagatgaga gtgcgctgct cgccggcgag cgcggcgagc
      541 gcggccgcct tctcgccttc cttgcgcggc gggaagccct cgaaggcaaa ccggtctgtg
      601 ggcaggccgg atagcgcgag cgcggccagc gcggccgacg gcccggggaa cacctcgatc
      661 ggggcatcgg ccacgatggc aaccgtcagc agccggtagc cggggtccga gaccagcggc
      721 attcccgcgt cggacaccac gaccacggtg tcgcccgcga gcacgcggtc caccagccgc
      781 tgagccacgt ggccctcgtt gtgctcatga agcgccacca gctcgccacc gatgtgtatc
      841 tgcgcacgca tggccagttc cttggtcacc cgggtgtcct cggccgccac cacgtcggcc
      901 gtcgcgaggg cctcccacag cctggcgctg ccgtcgcgga cattgccaat gggtgtggcg
      961 gcaagaatga ggcggcctgg catggctcca gactggcaca cgccgggacc gatgctgcgc
     1021 ctggtggtgc ccccgcccgt taggctcttg ccgtgatcct gaccctgtgg cgctggcgtg
     1081 caaccgtcat ggccactggc gtcaccctcc tcgccgccgt cctgcgtttc gcagatctgg
     1141 cccacccgcg ggcactcgtc tttgacgagg tgtattacgt ccgcggcgcc tactcgctcc
     1201 tcacgatggg ctacgagggc aactggggcg acgacaacgg ccacttcgca cagggcgact
     1261 tctcggatct cgagacggag ggcgactacg tcgtccaccc catggtcggc aagctgctga
     1321 tcgccggcgg catgaagctc ttcgggaacg ggccctttgg ctggcgtttt gccggggcct
     1381 tcctcggcac cgtgacggtg ctgatggtgg cgttgctgat gcgacggatc atgaagtcga
     1441 cgatgtgggg cggcgtcgcc ggtctcttgc tcgcgatcga tggcttgcat atcgtcatgt
     1501 cgcgcaccgc gatcctcgac gtcttcctca cgttcttcgt cgttgctggc ttcgccctgc
     1561 tgtggctgga tcgatggcgg gcgcaacgca tctacgacgc ccatcccgcc gacacggtgg
     1621 gcggtttcgg accccaggtg gggatccggt ggtggcgcct cttggcgctc gtcacgttcg
     1681 ggcttgcgac aggcgtgaag tggtcgggcc tgtactttgc ggcggcgttc atggtgctgt
     1741 tcgtggtcct ggacgcaggg gatcgcaagg ccgcggggta tcggcactgg ctatcggctg
     1801 cggcgttgcg gtccgcgctt cctgggctcc ttgcgaccct cgtcgtcctt cccaccgtgt
     1861 acgtcgcaca gtgggccaac tggttcgcca cagacggctc gtaccgccgt cactgggcgg
     1921 aagaccaccc tggcgagggc gtcacgtggc tgcccgatcc gttgcggtcg ctgtggagct
     1981 atcacgtgtc gatgtacaac ttccacaagg atctcgacag tccgcatccg tgggagtcgc
     2041 atcccgccgg ctggatcatc cagttccgcc ccacgtcgtt cttcttcgaa cgaatcgagg
     2101 acgcccgcc
//
LOCUS       DASRKH010000307         2528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_30312, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000307 DASRKH010000000
VERSION     DASRKH010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30312"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VFC06_04930"
     CDS             complement(<1..467)
                     /locus_tag="VFC06_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydD"
                     /protein_id="HZL81271.1"
                     /translation="MRPIDPRLIARIGPARRYVIVTAVLGFLTAFTILLQALLVSRML
                     APVLAPAPLTDDGLGPLGALVPLGARELAKGLPWLAIVVLARVFAVWVQERLAHRAGT
                     RVVSELREAVVAHATTMGPRWIASGKGAEVVTTVTRGLDGLLPYFVRYLPQLML"
     gene            complement(475..1506)
                     /gene="cydB"
                     /locus_tag="VFC06_04935"
     CDS             complement(475..1506)
                     /gene="cydB"
                     /locus_tag="VFC06_04935"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HZL81272.1"
                     /translation="MNLADFWFILIAVLWTGYLVLEGFDFGVGMLMPIVGKTSKGKRV
                     LINTIGPVWDGNEVWLLTAGGATFAAFPEWYATLFSGFYLPLLLILLALIVRPVAIEY
                     RSKIDDESWRNRWNLAIVISSWIPSVLWGVAFANIVKGVPIDAEGTYTGGFFNLLNPF
                     ALLGGLVTLAIFLSHGAIFLSLKTSGAVFERAKAFAKPASIAAVAVGAVWALWLQLAY
                     SENAWTWGALLLAAIGLLGAAWYSRNDKFGLAFTFNVVAIVGAVVLIFGSLYPDVMPS
                     TTDAAFSLTVDNARSSHTTLVVMTWVAGIFTPIVLAYQAWSYWVFKRRLSEDHMPEHN
                     ELKFFTKSS"
     gene            complement(1525..>2528)
                     /locus_tag="VFC06_04940"
     CDS             complement(1525..>2528)
                     /locus_tag="VFC06_04940"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HZL81273.1"
                     /translation="VGTEFNPEKGRAEMVNIWEVLGNNTTLLAFPHVISTAFLVAGAF
                     VAGIAAFWVVKKAKQDKTDSELPMYRTALRFGAVVMLIAGLAVIVTGDLQAKLMFKQQ
                     PLKMAAAEGLVDTSSGAPFSLLTIGNLAGDDPGSVKHIIEIPKLTSYLATGDFNSEVK
                     GLTQLQSEYAAKYPGYEATPDYLPNLAVTYWSFRFMIGLGAFSAAVALAALWLTRKSR
                     VTNKSWFSKLAVWAIPAPFLAASFGWIFTEMGRQPFVVAPNPTGSDMVYMLTQHGVSK
                     AVSAWEVGISMVAFTLVYAILAVFWYRLMVRYAQEGAPQMAEPEPSDDADRPMSFAY"
BASE COUNT          514 a          770 c          857 g          387 t
ORIGIN      
        1 agcatcaact gcggcaagta gcgcacaaag tacgggagca ggccgtcgag cccccgagtc
       61 acggtggtga cgacctcggc gccctttccc gacgcgatcc accggggccc catggtggtg
      121 gcgtgggcga cgacggcttc acgcagttcg gagacgacgc gtgtaccggc ccggtgagcc
      181 aggcgttcct gaacccacac ggcaaacacg cgcgccagca cgacaatcgc gagccacggc
      241 agtcccttgg cgagttcccg cgcgcccagg ggcaccagcg cgcccagggg gccgaggccg
      301 tcgtccgtca ggggtgcagg agccagcacg ggggccagca tccgagacac caagagtgcc
      361 tgcaggagga tggtgaacgc ggtcagaaaa ccaagcaccg ccgtcacgat cacgtaacgg
      421 cgggctggcc cgatccgcgc gatcaggcgc gggtcgatgg gtcgcataga gtccctacga
      481 actcttggta aagaacttca gctcgttgtg ctcgggcatg tggtcctcgg acagtcgacg
      541 cttaaacacc cagtaggacc acgcctggta ggcgaggacg atcggggtga agattccggc
      601 cacccacgtc atcaccacga gcgtggtgtg cgaggaccgg gcgttgtcga ccgtgagact
      661 gaaggccgcg tcggtggtgg acggcatcac gtcggggtag agcgacccga agatcagcac
      721 cacggcaccc acgatggcca ccacgttgaa ggtgaacgcg aggccgaact tgtcgttgcg
      781 gctgtaccag gcggcgccga ggaggccgat ggcggcgagc aggagagcgc cccacgtcca
      841 ggcattctcc gagtaggcca attgcagcca cagcgcccac acggcaccga ccgcgacagc
      901 ggcgatcgag gcgggcttgg cgaaggcctt ggcacgctcg aacactgcgc ccgaggtctt
      961 gagcgacagg aagatcgctc cgtggctgag gaagatcgcc agcgtcacta ggccgcccag
     1021 cagggcgaac gggttgagca ggttgaagaa gccacccgtg taggtgccct ccgcatcgat
     1081 cggcacaccc ttgacgatgt tggcgaacgc gacgccccac aggacggacg ggatccacga
     1141 ggagatcacg atcgccaggt tccagcgatt gcgccagctc tcgtcgtcaa tcttcgaccg
     1201 gtactcaatc gccaccgggc ggacgatcag cgccaacagg atgagcaaca ggggcaggta
     1261 gaagcccgag aagagcgtgg cgtaccactc ggggaaggcc gcgaacgtgg cgccaccggc
     1321 ggtgagcagc catacctcgt tgccgtccca cacggggccg atggtgttga tcaggacgcg
     1381 cttgcctttg gacgtcttgc cgacgatcgg catgagcatg ccgacgccga agtcgaagcc
     1441 ctcaaggacc aggtagccgg tccagaggac cgcgatcagg atgaaccaga aatctgcgag
     1501 attcatgagt gtgttccttc cggcctagta ggcaaacgac atcggtcggt cggcatcgtc
     1561 gcttggttcc ggctccgcca tttgaggcgc gccctcttgg gcgtagcgca ccatgaggcg
     1621 gtaccagaag acagcaagga tggcgtagac gagggtgaac gccaccatgg agatgccgac
     1681 ctcccatgcg gagacggcct tggacacgcc gtgttgggtg agcatgtaga ccatgtcgga
     1741 accggtggga ttgggcgcaa ccacaaaggg ttggcgcccc atttcggtga agatccagcc
     1801 gaaggaagcc gcgaggaagg gagccgggat ggcccacacc gcgagcttgg agaaccacga
     1861 cttgttcgtg actcggctct tgcgagtgag ccacagagcg gccagcgcca cggccgctga
     1921 gaaggcgccg agcccgatca tgaagcggaa cgaccagtac gtgacggcca ggttgggcag
     1981 gtagtcaggc gttgcctcgt agcccggata cttggccgca tattcagact gcaactgcgt
     2041 gaggcccttg acctccgagt tgaagtcgcc cgtggccaga tatgacgtga gcttggggat
     2101 ctcgatgatg tgcttgaccg agcccgggtc atcgccagcg aggttcccga tcgtcagcaa
     2161 tgagaacggt gcacccgagg aagtgtcgac gagtccttcg gcggcggcca tcttgagggg
     2221 ctgctgcttg aacatcaact tggcctgcag gtcgccagtg acgatcacgg cgaggccagc
     2281 gatgagcatc acgactgcgc cgaagcgcaa tgcggtgcgg tacatcggaa gctcgctgtc
     2341 cgtcttgtcc tgcttggcct tcttgactac ccagaaggcg gcgatgccgg cgacaaaggc
     2401 gccagccacc aggaaggcgg tggagatcac gtgcgggaag gcgagcagcg tcgtgttgtt
     2461 gcccagcacc tcccagatgt tcaccatctc ggcgcggccc ttctcggggt tgaactccgt
     2521 tcctacgg
//
LOCUS       DASRKH010000308         2455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_45324, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000308 DASRKH010000000
VERSION     DASRKH010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45324"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VFC06_04945"
     CDS             <1..410
                     /locus_tag="VFC06_04945"
                     /inference="COORDINATES: protein motif:HMM:NF013358.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HZL81274.1"
                     /translation="AGIWEFANRNTFGATAFCSYGAFWLSFWFLARFTDLSGAGSDAG
                     KGVGVFLLAWGIFTLYMTIAARRTSIAVFGVFVFLTVTFFALAAGDYTGTIGFTRVGG
                     GLGIATALLAWYASSAVVINSTHKRIVLPVGPQ"
     gene            complement(460..1776)
                     /locus_tag="VFC06_04950"
     CDS             complement(460..1776)
                     /locus_tag="VFC06_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002855226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4921 family protein"
                     /protein_id="HZL81275.1"
                     /translation="MPEPLVRLPDGTVKQVSPLTGTVVWTIPSRAHRPLGGPPEDRRP
                     VDASAAERLCAFCADRHLETPPEKSRLVVSPDGTWSVLRDVPAEHLTDTVPQFRRIPN
                     LFEILPLDYWRTNHGYVVPAQVLDRARAYTAVPQGRAHVLAILRGRLTAAGMTDDEWA
                     RAGEDAWLEHGVDLFAGSHDVIVARRHLVDGAVFDDELAGSGTLTPDEHHRYVAFTIQ
                     ALRELYDAQPHARYVAVFQNWLRPAGASFDHLHKQLVAIDEHGPSTDRVLDRLRDDPD
                     VFTTAVIDHAATHRLVVAENDHAIAVAGVGHRYPAIEVYSTSEHQRPWEHTDDEVRAV
                     SDLLHAVHAATGTHVPTNEEWHHRPLDVAEPMPWRIVLKWRVSTLAGFEGGTRIYVNT
                     IDPSTVRDQVVTELHRLRAVGAIGSMRLGDECSHRLGALGYARGGR"
     gene            1918..2277
                     /locus_tag="VFC06_04955"
     CDS             1918..2277
                     /locus_tag="VFC06_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012803884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZL81276.1"
                     /translation="MTTPVHQLKAELFKTLGHPLRIRILEMLCVREHSVGELLAELDV
                     EASNLSQQLGVLRASGLVATRRESSAVLYAVTDPEIAELLAVARRIRTRLLSDQLELL
                     SDRSPETSGQPTGASSR"
     gene            2274..>2455
                     /locus_tag="VFC06_04960"
     CDS             2274..>2455
                     /locus_tag="VFC06_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81277.1"
                     /translation="MSVLRKIIRTGRVAEPSLPVPSVDAAAGAAPAAPAAPGPLAGSV
                     QLRHIDAGSCNGCELE"
BASE COUNT          322 a          855 c          899 g          379 t
ORIGIN      
        1 ctgcggggat ctgggagttc gcgaaccgca acacgttcgg cgccacggcg ttctgctcct
       61 acggagcgtt ctggctgtcg ttctggttcc tcgcccggtt cacggacctg tcaggtgccg
      121 gctcggacgc aggcaagggc gtgggcgtgt tcctgctcgc ctgggggatc ttcacgctct
      181 acatgacgat cgcggcgcgc cggacgagca tcgcggtgtt cggagtcttc gtcttcctga
      241 ccgtgacgtt cttcgcgctc gctgccggtg actacacggg gaccatcggg ttcacgaggg
      301 tcggtggcgg gctggggatc gccaccgcgc tgctggcctg gtacgcatcg tctgcggtgg
      361 tcatcaactc cacccacaag cggatcgtgc tgcccgtcgg tccccagtag ctcctcgggg
      421 cgcgaggccc gcagcggtcg ctgaccttcg agcgtcccgt cagcggccgc cgcgggcgta
      481 gccgagcgcc ccgagccggt gcgagcactc gtccccgagg cgcatcgacc cgatggctcc
      541 gaccgcgcgc aaccggtgca gctcggtgac gacctgatcg cgcaccgtgg acgggtcgat
      601 ggtgttgacg tagatccgcg tgccgccctc gaagcccgcg agggtcgaca cgcgccactt
      661 gagcacgatg cgccacggca tcggctcggc gacatcgagc gggcggtggt gccactcctc
      721 gttcgtcggc acgtgcgtcc cggtcgcggc gtgcacggcg tgcagcaggt cggagacggc
      781 gcgcacctcg tcgtcggtgt gctcccacgg ccgttggtgc tcgctggtcg agtacacctc
      841 gatcgccggg tagcggtgcc cgacgcccgc cacggcgatc gcgtggtcgt tctccgcgac
      901 gacgagccgg tgcgtcgcgg cgtggtcgat gacggcggtg gtgaacacgt ccgggtcgtc
      961 gcgcagccgg tcgagcacgc ggtcggtcga cgggccgtgc tcgtcgatcg cgaccagctg
     1021 cttgtgcagg tggtcgaagg acgcccccgc gggccgcagc cagttctgga acacggcgac
     1081 gtagcgggcg tgcggctgcg cgtcgtacag ctcgcgcagg gcctggatcg tgaaggccac
     1141 gtaccggtgg tgctcgtccg gggtgagggt gcccgagccg gcgagctcgt cgtcgaacac
     1201 ggcgccatcg acgaggtgac gtcgcgcgac gatcacgtcg tgggacccgg cgaacaggtc
     1261 gacgccgtgc tcgagccacg cgtcctcgcc cgcccgggcc cactcgtcgt cggtcatgcc
     1321 ggccgccgtc aggcgcccgc gcaggatcgc gaggacatgg gcgcgtccct ggggcaccgc
     1381 ggtgtaggcg cgggcgcggt cgagcacctg ggccggcacg acgtagccgt ggttggtgcg
     1441 ccagtagtcg agcggcagga tctcgaacag gttcgggatc ctgcggaact gcggcacggt
     1501 gtccgtgagg tgctccgcgg gcacgtcacg cagcacggac cacgtcccat cgggggagac
     1561 gacgaggcgg gacttctccg gcggggtctc caggtggcgg tccgcgcaga acgcacagag
     1621 ccgctcggcg gccgacgcgt cgacgggtcg ccggtcctcg ggcggtccac ccaggggtcg
     1681 gtgcgcgcgg ctcgggatcg tccacacgac cgtcccggtc agcggtgaga cctgcttgac
     1741 ggtgccgtcg ggcaaccgga cgaggggttc gggcacacca gcaccctaga gccgggcggc
     1801 accgagcggc tctcgcactt gcagagttct gcaactgatc atcatgtggc atgatctggt
     1861 gtgcccggtg ggcggccatg acggctcccg cggcgctgac gacgagagag ggccacgatg
     1921 acgactcccg tccatcagct gaaggccgag ctgttcaaga cgctcgggca ccccctgcgg
     1981 atccgcatcc tcgagatgct ctgcgtccgg gagcactccg tcggcgagct gctggccgag
     2041 ctcgacgtcg aggcgtccaa cctgtcgcag cagctcggcg tgctcagagc atcggggctc
     2101 gtggcgacgc gccgggagtc gtccgcggtc ctctacgcgg tgaccgaccc cgagatcgcc
     2161 gagctgctgg ccgtggcccg ccgcatccgg accaggctgc tgtccgacca gctcgagctg
     2221 ctcagcgacc ggagccccga gacctccggc cagccgacag gtgcgtcctc ccggtgagcg
     2281 tgctgcgcaa gatcatccgc accggccggg tcgccgagcc ctccctcccg gtgccttcgg
     2341 tcgacgccgc agcaggtgcc gcgcccgccg cgcccgccgc acccggcccg ctcgcgggct
     2401 cggtccagct gcgtcacatc gacgcgggct cgtgcaacgg gtgcgagctg gagat
//
LOCUS       DASRKH010000309         2093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_32015, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000309 DASRKH010000000
VERSION     DASRKH010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32015"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2093
                     /locus_tag="VFC06_04965"
     CDS             <1..>2093
                     /locus_tag="VFC06_04965"
                     /inference="COORDINATES: protein motif:HMM:NF019974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZL81278.1"
                     /translation="ALRDDVRNATVLPGTVDTLYTGIVNGVLAMTRGVADSSADRQLA
                     TYLTAYASMNELFEAVSLEENYVTRKIRDGGWSFGEPARFQRLYFSTTNALENAKLAV
                     NPLPNIDEVPSFGASFDVAETNGFQVIRDRLVVDATDEGLLDQRSADWQVRVAEELAV
                     MEPIRAELLTEVTQEASDLVRNSLVQLLATIAVAAAVFTVSLVVTLSMARRIINPLRR
                     LTTTATAVRQELPRLVERVALPGQSVDVSEVQIPVESQDEVGRLAEAFNSVNAATLSI
                     AAEQAALRGSISEMFVNVARRDQVLLNRQLSSIDEMERTEDDQTTLTKLFALDHLATR
                     MRRNSESLLVLAGIDTGRRLRRSMPLSDVIRTASSEIELYERVQLELDADPSMLGHSA
                     LTAAHMFAELLENATVFSDPGTPVVVRTMTQGDDICVEVVDYGIGMTPEELGEANARV
                     ASTAASEILGAQRLGLFVVGRIARRVGARVQLESIEGGGICARVVMPPSLFDPTAPSD
                     QGHLSSTAVDEVTHAPAALVHHSVSDAEVRDPIPTEAQRPIAPLHGYQPTVIGGGADL
                     AGRAIEPPVEAESPVAAESPVAAEAAGVLPDGAPSGIESLVASDAESAPEAVPVDLDA
                     LTDGATSTGLPTRRRKRGDEGPEAPQETHSIMALPMRASDAQLTALESATTPGFTPIL
                     AADEVSPESAEQRARV"
BASE COUNT          363 a          698 c          663 g          369 t
ORIGIN      
        1 gccctgcgag acgacgtccg caacgccacc gtgcttccgg gtaccgtcga caccctctac
       61 accgggatcg tgaacggcgt tcttgccatg acgcgtggcg ttgccgactc gtccgccgac
      121 cgacagttgg ccacttactt gacggcatac gcatcgatga acgaactttt cgaggcggtc
      181 agtctcgaag agaactacgt gacgcgcaag attcgtgacg gcggttggtc ctttggtgaa
      241 cctgcacggt tccagcgcct gtacttcagc acaaccaacg ctctcgaaaa tgcgaagctc
      301 gcggtcaacc ccttgcccaa catcgacgaa gttccgtcgt tcggcgcgtc gttcgatgtc
      361 gcagaaacca acggcttcca ggtgatccgt gaccgcctgg tggtcgacgc gacggatgag
      421 ggattgcttg accagcgctc ggcggactgg caagttcggg tggcagaaga gctcgctgtg
      481 atggagccga tccgcgccga actcctcacc gaggtgacgc aggaagcctc ggacctggta
      541 cgaaacagcc tggtccaatt gctcgcgacg atagccgttg ccgcggccgt cttcaccgtg
      601 tcgctcgtcg tcacgctgtc gatggcacgc cgcattatca accctctgcg gcgcctgacc
      661 acgacggcta ccgccgtgcg acaagagctg ccgcgactcg tggagcgggt ggcgttgcct
      721 ggtcagagcg tcgacgtttc cgaagtgcag atccccgtgg agtcgcagga cgaggtcggg
      781 cgtctagcag aggcgttcaa ctccgttaac gcggcaaccc tgtccatcgc cgccgaacag
      841 gccgcgctgc gtggttccat ctcggagatg ttcgtcaacg tggcccgtcg tgaccaggtt
      901 ctcctcaatc gccagctctc gtcgatcgac gagatggagc gcaccgaaga cgaccagacc
      961 accctcacca aattgttcgc cttggatcac ctggcaacgc gaatgcgccg taacagtgag
     1021 tcgctgctgg tgcttgcagg catcgacact ggtcgacgcc tgcgtcgttc catgccgctt
     1081 tccgacgtga tccgcacggc gtcatccgag atcgagttgt acgagcgagt tcagttggaa
     1141 ctcgacgccg acccgtcgat gcttggccac tcggccctca ccgcagcaca catgtttgct
     1201 gaactcctgg aaaacgcgac ggtcttctcg gatcctggaa cgccggtcgt ggtgcggacc
     1261 atgactcaag gggacgacat ctgcgttgag gtcgttgact acggcatcgg catgacgccc
     1321 gaggaactgg gcgaggccaa cgcccgcgtt gcttcgacgg cggcctcgga aattcttggt
     1381 gcgcagcgtc tgggcctctt cgtggtcggc cgtattgccc gccgcgtggg cgctcgtgtg
     1441 cagttggagt ccatcgaggg cggaggaatc tgcgcccggg tcgtgatgcc accatcactg
     1501 ttcgatccga ctgccccaag cgatcaggga cacctgtcga gcaccgccgt cgacgaggtc
     1561 acccacgccc ccgcggctct ggtgcaccac agcgtgtccg acgccgaggt gcgcgatccc
     1621 attcccaccg aggcgcagcg tccgatcgcg ccactgcacg ggtaccagcc gaccgtcatc
     1681 ggaggcggtg ccgaccttgc tggccgcgcg atcgagcctc cggtagaggc tgagtctccg
     1741 gtagcggccg agtctccggt agcggccgag gccgcaggcg ttctgccgga cggggcccca
     1801 tcgggaatcg agagccttgt cgcctccgac gcggagtccg cacccgaagc cgtgcccgtc
     1861 gatcttgacg cgctcaccga cggcgcgacg tccaccgggc tgcccacacg tcggcgcaag
     1921 cgcggcgacg aaggccctga ggcgccgcag gaaacccaca gcatcatggc cttgcccatg
     1981 cgggcctccg acgcgcagtt gactgccttg gagagcgcca cgacgccggg gtttacgccg
     2041 atcctcgcgg ccgacgaagt gtcgccggag agcgccgagc agcgcgccag ggt
//
LOCUS       DASRKH010000310         2158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_40011, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000310 DASRKH010000000
VERSION     DASRKH010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40011"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /gene="prmC"
                     /locus_tag="VFC06_04970"
     CDS             <1..831
                     /gene="prmC"
                     /locus_tag="VFC06_04970"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HZL81279.1"
                     /translation="PRDATRLARALLARHAGDGALLELVDIPSAALERDLRAAVARRA
                     AREPLQLIVGYVDFRGATIATAPGVFIPRFETELVCGEAVAAARALAGRDEPVRVVDL
                     CTGSGAIATAIAREVPGCLVWAVEQSTPALALAARNAAANGVAVTVVAGDAADALGEL
                     DGTVDVVVSNPPYIPPDGVPRDPEVLEWDPPTALYGGGADGLDVPRSVMRSARRLLRP
                     GGVLVMEHADVQGPAVRHDLALLGGFTVIVTLPDLTGRDRMVRATRLAGAPQVRDWHT
                     "
     gene            828..1541
                     /locus_tag="VFC06_04975"
     CDS             828..1541
                     /locus_tag="VFC06_04975"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HZL81280.1"
                     /translation="MILSYLDEPAAALDAAVRALEKGGLVVLPTDTVYGIAADAFSPT
                     AVGRLLAAKGRGRQMPPPVLVANPGMVNALAVDVSHDARALLQAFWPGALTIILRSQP
                     SLAWDLGETSGTVALRMPDNEGALALLQRYGPLAVTSANLTGQPPAQDAQAAEAYFGA
                     NVAVYVDAGPTPGPTASTIVDATGPVMRIVRAGVLTRAQLAEVVPALAEEGAEPGAGD
                     DSGGDAEPGSDDSPGAEGA"
     gene            1538..>2158
                     /locus_tag="VFC06_04980"
     CDS             1538..>2158
                     /locus_tag="VFC06_04980"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015881790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HZL81281.1"
                     /translation="MRTYLLVMAIAAAVTFICTPIARHLARYSRAITPVRSRDVHTSP
                     IPRMGGVAMMVGLAVALLCAARIPFLSPVFTQTGQASGLILAAVWVCLLGAADDLWDL
                     DWWAKLGGQVIAGLVLAWSGVLLVTFPIAGLTIGSPRLSVVATVFVVVAAINAVNFVD
                     GLDGLAAGMIGIGGAAFFAYTYILTRVSSPEDFSSLATAILAALVGV"
BASE COUNT          259 a          812 c          771 g          316 t
ORIGIN      
        1 ccccgggacg ccactcggct cgcccgcgcc ctcctcgcgc gccatgccgg cgacggcgca
       61 ctgctcgaac tcgtggacat cccgtccgcc gccctcgaga gagatctgcg cgccgcggtc
      121 gcccgccggg cggcccgcga accgctgcag ctcatcgtgg ggtacgtcga cttccgcggc
      181 gccacgatcg cgacggcgcc gggcgtcttc atcccacgct tcgagaccga actggtctgc
      241 ggggaggcgg tcgcggcggc ccgcgcgctg gccggacgcg acgaaccggt gcgcgtcgtc
      301 gacctgtgca ccgggagcgg cgccatcgcc accgcgatcg cgcgcgaggt cccgggatgc
      361 ctcgtgtggg cggtcgagca gtcgacacca gccctcgccc tcgccgcccg caacgcggcg
      421 gcgaacggcg tcgcggtgac ggtggtggcg ggtgacgccg cggacgcact cggcgagctc
      481 gacgggacgg tcgacgtcgt cgtctccaac ccgccctaca ttccccccga cggcgtgccg
      541 cgcgatcccg aggtcctcga gtgggacccg cccaccgccc tctacggcgg cggcgcggac
      601 ggcctcgacg ttccgcgctc cgtgatgcgt tcggcccgcc ggctgctgcg gccgggcggc
      661 gtcctcgtga tggagcacgc cgacgtccag ggaccggccg tgcgccacga ccttgcgctc
      721 ctcggtggct tcaccgtgat cgtcaccctt ccggacctca ccggccggga caggatggtg
      781 cgcgccacgc gcctcgcggg cgctccccag gtgcgagact ggcacacgtg atcctctcgt
      841 acctcgacga gcccgccgcc gcactcgacg cggcggtccg cgccctggag aagggcggac
      901 tggtggtcct ccccaccgac acggtgtacg gcatcgcggc cgacgcgttc tccccgacgg
      961 cggtcgggcg gctgctggcg gcgaagggac ggggacggca gatgccccca ccggtcctcg
     1021 tcgcgaaccc cggcatggtc aacgcgctgg ccgtggacgt cagccatgac gcgcgggcac
     1081 tgctgcaggc cttctggccg ggggcactga cgatcatcct gcgctcacaa ccgtccctgg
     1141 cgtgggacct cggcgagacc tccggcacgg tggcgctgcg catgcccgac aacgaggggg
     1201 cgctcgcact gctgcagcgc tacggcccgc tcgcggtcac gagcgccaac ctgaccgggc
     1261 agcccccagc ccaggacgca caggccgcgg aggcctactt cggggcgaac gtcgcggtgt
     1321 acgtggacgc ggggccgaca ccgggcccca ccgcgtccac gatcgtcgac gcgaccgggc
     1381 cggtgatgcg gatcgtgcgc gccggcgtac tcacccgggc ccaactcgcg gaggtcgtcc
     1441 ccgcgctcgc cgaggaaggc gctgagcccg gggccggtga cgattcgggt ggcgatgcgg
     1501 agcccggttc cgacgacagt ccgggggccg aaggcgcgtg aggacgtacc tgctggtgat
     1561 ggccatcgcg gcagcggtga ccttcatctg cacgcccatc gcccgccacc tcgcgcgtta
     1621 ctcccgtgcc atcacccccg tgcggtcgcg tgacgtccac accagcccaa tcccgcggat
     1681 gggtggggtg gcgatgatgg tgggccttgc cgtcgcgctg ctgtgcgccg cccggatccc
     1741 gttcctcagc ccggtgttca cccagaccgg acaggcgtcc gggctgattc tcgccgccgt
     1801 gtgggtgtgc ctcctcggcg ccgcggacga cctctgggac ctcgactggt gggccaaact
     1861 cgggggccag gtcatcgcgg gcctggtgct cgcgtggagt ggcgtgctgc tcgtgacctt
     1921 cccgatcgcc gggctcacga tcggctcccc ccggctctcg gtggtggcca cggtgttcgt
     1981 ggtggtggca gcgatcaacg ccgtcaactt cgtggacggt ctggacggtc tcgctgccgg
     2041 gatgatcggg atcggcgggg cggcgttctt cgcctacacc tacatcctca cgcgtgtgag
     2101 ttcgcccgag gacttctcct cgctcgcgac cgcgatcctc gcggccctcg tgggggtg
//
LOCUS       DASRKH010000311         2586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_14293, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000311 DASRKH010000000
VERSION     DASRKH010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14293"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VFC06_04985"
     CDS             <1..355
                     /locus_tag="VFC06_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="globin"
                     /protein_id="HZL81282.1"
                     /translation="RGQHLVRAHAVVEATDEVLSPLYPAHDMDGAVERLTMFLEQYWG
                     GPTTYSDQRGHPRLRMRHQPFHVNPEARDHWMAHMRAAVEASDLSPLHAATLLDYLER
                     AAHSMINTFDPTPGR"
     gene            527..1411
                     /locus_tag="VFC06_04990"
     CDS             527..1411
                     /locus_tag="VFC06_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HZL81283.1"
                     /translation="MEHVEDIWGDAAVAAGLGSLNLRRLGDTSFEGDSLPMPGGRVFG
                     GQVIAQAVLAAGHTVDESRIVHSMHGYFLRPGDASRPIRFEVEDLRDGRSFSARRTHA
                     FQDKKPILSMIASFQEAQPGPEHSVRMPEVPPPDALPSAMDVLAPFEGHPTADFWLNS
                     APFDVRHVEGSIFIEPDKAPKQYQTAWMRVRRTLDVSDLMHRALLAFGSDQVILEPAL
                     RCHGASWAMPGVAFASLDHALWWHRPARADEWLLFVQDAPTAQSGRGMCGSWVFSEDG
                     RLVASVAQEGMLRMPEGD"
     gene            1681..>2586
                     /locus_tag="VFC06_04995"
     CDS             1681..>2586
                     /locus_tag="VFC06_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013348874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HZL81284.1"
                     /translation="MTETTALKVDGLYKVFGKHAESAVRKLEDGATRDSLGGSTTAAV
                     IDTSFEVQRGEIFVVMGLSGSGKSTLIRMLNGLHEATAGTVEIEGEQVTGATTKLLRD
                     IRRKRMSMVFQHFALLPHRTVLDNVAYGLEIQGMPQAEREAKAREITQVVGLAGWEDK
                     HPSELSGGMQQRVGLARALAADTEVLLMDEAFSALDPLIRREMQEQLLELQSTLGKTI
                     VFITHDLNEAMFLGDRIAVMRDGRIVQIGTPEEILTDPANDYVEQFVQDVDRTRVLTA
                     ESVMRKPRAVVHPTSGPHGALKTMRD"
BASE COUNT          454 a          886 c          818 g          428 t
ORIGIN      
        1 gcggggacag cacctcgtcc gcgcgcacgc cgtcgtagaa gcgacggacg aggtgctgtc
       61 cccgctgtac ccggcgcacg acatggacgg cgcggtcgaa cgactcacga tgttcctcga
      121 gcagtactgg ggcgggccca ccacgtactc cgaccagcgc ggacacccgc ggttgcgcat
      181 gcgccaccag ccgttccacg tgaaccccga ggcccgcgac cactggatgg cgcacatgcg
      241 cgcggccgtc gaggcgtcgg atctgtcgcc cctgcacgcg gcgacacttc tcgactacct
      301 cgagcgcgcc gcccactcga tgatcaacac gttcgaccca acgccgggtc gctgacgggg
      361 tggtggtgcc gatgcgtccg cggctggcct cccctcctgc ggccgacctt ccgtgcagaa
      421 ctcactactg atgcgacatg gcggtgccga tccacgctcg cgccaccgcc ggactgtccg
      481 cctggtagtg agttctcaac aggtgtcggc caggtagcct cgacgcatgg agcatgtgga
      541 agacatctgg ggtgacgctg cggtagccgc gggactgggc tcactcaacc tgcgcagact
      601 gggcgacacc agctttgaag gcgattcgct gccgatgccg ggcggccgag tgtttggcgg
      661 ccaggtcatc gcccaggcgg tgcttgcggc gggtcacacc gtcgatgagt cccgaatcgt
      721 gcactcgatg cacggctact ttctgaggcc gggcgacgcg agccgcccga ttcgcttcga
      781 ggtggaggac ctgcgtgacg gcagatcgtt ctccgcgcgc cgtacccacg cgttccagga
      841 caagaagccg atcctgtcga tgatcgcgtc cttccaggag gcccagcccg gccccgaaca
      901 ctcggtgcgg atgcccgagg tgccaccgcc ggacgcgttg ccgtccgcaa tggatgtgct
      961 cgccccgttc gagggccacc ccaccgcaga cttctggctc aactccgcgc ccttcgacgt
     1021 gaggcacgtc gaaggctcga tcttcatcga gccagacaag gcgcccaagc agtaccagac
     1081 ggcatggatg cgcgtacgcc gcactctcga cgtgagcgac ctcatgcatc gggcactcct
     1141 ggcgttcgga tccgaccagg tgatcctgga gcccgcgctg cggtgtcacg gcgcctcgtg
     1201 ggcgatgccc ggcgtggcgt tcgccagcct ggaccacgcc ttgtggtggc atcgcccggc
     1261 ccgcgccgac gaatggctcc tgtttgtcca ggacgccccc accgcccaga gcggcagagg
     1321 catgtgcggt tcctgggtct tcagcgagga cggacgcttg gtcgcgtccg tcgcccaaga
     1381 gggcatgctg cgcatgcccg agggagacta gctaagcgat ttaccaggga aagttgtttc
     1441 agcggcgcca ccttgctacg ttggtgaggc cgacatgttg atgcgcttcg cgtttccatg
     1501 atggcagaca aacccaggtc gccgtgcatc gctttgttgc aagtgcactg aagcctggct
     1561 acgcaggccc cgccatcggg gttgcgggtc cgtgaatgcc gtccgacggc tgcgattccc
     1621 tcgtggtcag gagcggccgc aatactgagt cagccctcgg gtcgctcact tgaggatgac
     1681 gtgacagaaa ccactgcgtt gaaggtcgac ggcctctaca aggtctttgg aaagcacgcc
     1741 gaatccgccg tgcgcaaact cgaagatggc gccacccggg actcactcgg cggaagcacc
     1801 accgctgccg tgatcgatac ctcattcgag gtccagcgcg gcgagatctt tgtggtgatg
     1861 ggcctgtccg gatccggaaa gtccacgctg atccgcatgc tgaacggcct gcacgaggcc
     1921 accgccggaa cagtcgaaat cgagggtgag caggtcaccg gagccaccac caagctcttg
     1981 cgcgacatcc gccgcaagcg catgtcgatg gtgttccaac acttcgcgct gctgccccac
     2041 cgcacggtgc tcgacaacgt cgcatacggc ctagagattc agggaatgcc ccaggccgag
     2101 cgtgaggcca aggcccgcga aatcacccag gtggtcggct tggccggctg ggaagacaag
     2161 cacccgagcg aactttccgg tggcatgcag cagcgcgtgg gcctagcccg tgcgctcgcg
     2221 gccgacaccg aggtcctcct catggacgag gcattctccg ctctcgaccc cctcatccgc
     2281 cgcgagatgc aagagcagct gcttgaactt cagtccaccc tcggcaagac gatcgtcttc
     2341 atcacacacg acctcaacga ggcaatgttc ttgggcgacc gcatcgccgt catgcgtgac
     2401 ggtcggatcg tccagatcgg cacccccgaa gagatcctga cggaccccgc gaatgactac
     2461 gtcgagcagt tcgtgcaaga cgtggaccgc acccgagtcc tcaccgccga gtccgtcatg
     2521 cgcaagccgc gggccgtcgt gcacccgaca tccggtccgc acggcgcgct caagaccatg
     2581 cgcgac
//
LOCUS       DASRKH010000312         2445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_61710, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000312 DASRKH010000000
VERSION     DASRKH010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61710"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="VFC06_05000"
     CDS             <1..757
                     /locus_tag="VFC06_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HZL81285.1"
                     /translation="TQIGTETAVLLYFRRDIARICTAWWRAVRGDHGTDLRSRMGAPA
                     GSPLDRDALMAWFIALGSIPIVVLGLAFKDSIESSFRNLWLIVLTLVVFGLILGWADR
                     VGAKHRSLDELTPRHAVAFGFAQSLALIPGVSRSGGTITAGMLLGYTRESAARYSFLL
                     AVPAVLGSGLYELAKSVGDFGKAGTPGLGVTLIATVVAFAVGYVVIIAFLRLISTRSF
                     MPFVVYRIGLAVVVVVLLLTGALDAIGAAPVAG"
     gene            845..2107
                     /gene="mshC"
                     /locus_tag="VFC06_05005"
     CDS             845..2107
                     /gene="mshC"
                     /locus_tag="VFC06_05005"
                     /EC_number="6.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--1-D-myo-inosityl
                     2-amino-2-deoxy-alpha-D-glucopyranoside ligase"
                     /protein_id="HZL81286.1"
                     /translation="MLTWPPPQIPRLPGRGGPVRVRDTSTGELVVAAAGDTATLYVCG
                     VTPYDATHLGHAATYVAFDLLVRSWRDAGQRVRYASNVTDVDDPLLERAHATGVDWRD
                     LAVEQTALFAEDMTALGVVPPDVYTGAVESIPAVAQAVRDLLAAGAAYRVEDPDTTGH
                     GRGDIYADLSADPDLGRVAGLDRAEMLALFAERGGDPARAGKRDALDPLLWRRGRPGE
                     PAWDGGELGSGRPGWHIECAVIARDGLGLPFDVQGGGIDLRFPHHEMSTSHARLLDAG
                     QGARIHVHAGMVGLDGEKMSKSRGNLVLVSRLRALGENPMAVRLSILAHHYASDWEWT
                     DADLDLARARLARWQRAVSGNGGPDATATLAAVRAALGADLDAPAALAAVDAWAVDAL
                     GAGGAADTVIGAPGVISRTVNALLGVRL"
     gene            complement(2111..>2445)
                     /locus_tag="VFC06_05010"
     CDS             complement(2111..>2445)
                     /locus_tag="VFC06_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HZL81287.1"
                     /translation="AEVRGMRALSLWAAVPHYVAHPPSPKATLAILTRLEGLLGEPIA
                     LGELPDDSIAWQAGVDELASEDSEIAEYVHQLEQAKDTADLPEASGEAIAQEFERYLR
                     RRDKGTGG"
BASE COUNT          287 a          908 c          884 g          366 t
ORIGIN      
        1 cacccagatc gggacggaga ccgcggtcct gctgtacttc cggcgcgaca tcgcccggat
       61 ctgcacggcc tggtggcggg cggtccgcgg tgaccacggc accgacctcc gctcgcggat
      121 gggtgcgccg gcggggtcgc ccctcgaccg ggacgcgctg atggcgtggt tcatcgcgct
      181 cggcagcatc ccgatcgtcg tgctggggct ggcgttcaag gactcgatcg agagctcgtt
      241 ccgcaacctg tggctgatcg tgctcacgct ggtcgtgttc ggcctgatcc tcgggtgggc
      301 ggaccgcgtg ggagcgaaac atcgctcgct cgacgagctg acgccccgcc acgcggtggc
      361 gttcgggttc gcccagtccc tcgcgctcat ccccggcgtc tcgcgctcgg gcggcaccat
      421 caccgcgggc atgctgctcg gctacacgcg cgagtccgca gcccggtact cgttcctgct
      481 cgcggtcccg gccgtgctcg gctcgggcct ctacgagctc gcgaagagcg tgggcgactt
      541 cggcaaggca gggacacccg gactcggtgt gacgctcatc gcgacggtcg tggccttcgc
      601 tgtcggctac gtcgtcatca tcgcgttcct gcggctgatc tcgacgcgca gcttcatgcc
      661 gttcgtcgtc taccggatcg gcctggccgt cgtcgtcgtc gtgctcctgc tcacgggcgc
      721 gctcgacgcg atcggggctg cgccggtcgc aggctgagcc gccgcaggcc gagccagccg
      781 cgaccacagg gtcgcgcacc ggacggtcgc ggtgcgggtg gccggtgccc gttaggctgc
      841 cgctgtgctc acctggccgc ccccgcagat acctcggctc cctgggcgcg gcggccccgt
      901 ccgggtacgg gacacgtcga ccggcgagct cgtcgtcgca gcagccggtg acactgcgac
      961 cctttacgtg tgcggcgtca cgccgtacga cgcgacccac ctcggccacg ccgcgacgta
     1021 cgtcgccttc gacctgctcg tgcggtcctg gcgtgacgcc ggtcagcgcg tgcggtacgc
     1081 ctcgaacgtc accgacgtcg acgacccact cctcgagcgt gcgcatgcga ccggagtcga
     1141 ctggcgcgac ctcgcggtcg agcagacggc gttgttcgcc gaggacatga cagcgctcgg
     1201 cgtcgtcccg ccggacgtgt acaccggcgc cgtcgagtcg atccctgccg tcgcgcaggc
     1261 cgtccgtgac ctcctcgcgg ccggcgccgc gtaccgcgtc gaggaccccg acaccaccgg
     1321 gcacggccgc ggcgacatct atgcggacct cagcgcggac ccggacctgg gccgtgtcgc
     1381 gggcctggac cgtgccgaga tgctggcgct gttcgccgag cgtggtggcg atccggcccg
     1441 cgcgggcaag cgcgacgccc tcgacccgct cctgtggcga cgggggcgtc cgggagagcc
     1501 cgcgtgggac ggcggggagc tcggcagcgg ccgaccgggt tggcacatcg agtgcgcggt
     1561 catcgcgcgc gacggcctcg gcctgccgtt cgacgtgcag ggcggcggca tcgacctgcg
     1621 gttcccgcac cacgagatga gcacgtcgca cgcgcggctg ctcgacgcgg gtcagggtgc
     1681 ccgcatccac gtgcacgcgg gcatggtcgg gctcgacggc gagaagatga gcaagtcgcg
     1741 cggcaacctc gtgctcgtct cccggctgcg tgcgctcggt gagaacccca tggccgtccg
     1801 gctctcgatc ctcgcgcacc actacgcgag cgactgggag tggaccgacg ccgacctcga
     1861 cctcgcccgc gcgcggctcg cccggtggca gcgggccgtc tccgggaacg gtgggccgga
     1921 cgcgaccgcg acgctcgcgg ccgtccgggc ggcactcggc gcggacctcg acgcaccggc
     1981 cgcgctcgcg gccgtcgacg cctgggccgt cgacgcgctc ggagccggcg gcgcggccga
     2041 cacggtgatc ggtgcgccgg gcgtcatctc tcggacggtc aacgcgctgc tgggcgtccg
     2101 gctgtagccg tcagccgccg gtgcccttgt cgcggcgccg caggtaccgc tcgaactcct
     2161 gcgcgatggc ctcaccgctc gcctcgggca ggtccgcggt gtccttcgcc tgctcgagct
     2221 gatggacgta ctcggcgatc tcgctgtcct cgctcgcgag ctcgtcgacc ccggcctgcc
     2281 acgcgatcga gtcgtcgggc agctcaccga gcgcgatcgg ctcacccagg agcccctcga
     2341 gacgcgtgag gatcgcgagc gtcgccttgg gcgacggggg gtgcgcgacg tagtgcggca
     2401 cggcggccca caacgacagc gcgcgcatgc cgcggacctc ggcct
//
LOCUS       DASRKH010000313         2212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_44895, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000313 DASRKH010000000
VERSION     DASRKH010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44895"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..505)
                     /locus_tag="VFC06_05015"
     CDS             complement(140..505)
                     /locus_tag="VFC06_05015"
                     /inference="COORDINATES: protein motif:HMM:TIGR03987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsmA family protein"
                     /protein_id="HZL81288.1"
                     /translation="MSPLASTFITLALVFYTVGVWGERIQKRLRWWHFAFFVLGLMFD
                     TLGTGLMFEFVGGMTFDVHGISGLIAILLMVVHAAWALVVLRRQDEDALKNFHKFSVF
                     VWLVWLIPYFSPMFFAMAE"
     gene            complement(549..773)
                     /locus_tag="VFC06_05020"
     CDS             complement(549..773)
                     /locus_tag="VFC06_05020"
                     /inference="COORDINATES: protein motif:HMM:NF022577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HZL81289.1"
                     /translation="MSALLNFLASQTGRYVRSGLGVILLVLAFVAGGAWAWVLGVLGV
                     VFLLVGILDVCLLAPLFGKPLRGKEFRSGV"
     gene            complement(801..1553)
                     /locus_tag="VFC06_05025"
     CDS             complement(801..1553)
                     /locus_tag="VFC06_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81290.1"
                     /translation="MSADLTTRYLGLELSGPIMASAGPLTGTLDGLRALEDSGASAVV
                     LPSLFEEDVIEEELHLADLLDDGEGFAEFASAPLSEAAVTVPLTVKLSPFYTSVAHFA
                     AAVVGRGADGLVLFNRFYAPDIDLDTLTVRPALTLSESDDLRLPLRWLGILRPQLPET
                     SLAASSGVHTGADALKALLVGADVACTTSSVLRRGPGHVTTMLAEVREWLEANEYESV
                     RQLRGSMSAASVPDPAGFERAQYRAIVTTLGR"
     gene            complement(1550..>2212)
                     /locus_tag="VFC06_05030"
     CDS             complement(1550..>2212)
                     /locus_tag="VFC06_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZL81291.1"
                     /translation="AYDIGFGGLDHVLGSGRNVNVLVLDTEVYSNTGGQASKATPRGA
                     AAKFAASGKDHPKKDLGMVAAAYGDVYVAQLAIGANQQQTVRAMLEAAAWPGPSLLIA
                     YSTCIAHGIDMGTSMTHQTDAVASGYWPLYRYRPGTDEHTQPMSLDSKAPTKPVTDFM
                     MAETRFSMLARSNPQRAEMLARLAQRDVDERWRYYSQLAGVERAVQVDTPAGPDDEKG
                     TE"
BASE COUNT          372 a          744 c          782 g          314 t
ORIGIN      
        1 cactccgcca acgcggcgga gctccacccg acgacccacc tggcgagtct ccaccggacg
       61 ctttcgaagg ccctccaccg ttctgacacg gcgagccgct ggactggaac ccgatgggtc
      121 gggcgtggtt cgtcggtgct cactccgcca tggcaaagaa catcgggctg aagtagggga
      181 tcagccagac cagccacacg aagacgctga acttgtggaa gttcttgagt gcgtcctcgt
      241 cctgtcgacg gagcaccacg agggcccacg cggcgtggac caccatgagg aggatggcga
      301 tcagcccgga gatgccgtgg acgtcgaagg tcatcccgcc aacgaactcg aacatcaggc
      361 cggtgccaag ggtgtcgaac atgagcccga ggacgaagaa cgcgaagtgc caccacctca
      421 ggcgcttctg gatgcgctca ccccacacgc cgaccgtgta gaacacgagg gcgagggtga
      481 tgaaggtgct ggcgagcgga ctcatggggt ctcctgggga gcgggacgcc gcgccgccgt
      541 cgagccggtc agacccccga gcggaattcc ttgccccgga gcggcttgcc gaacagcggg
      601 gcgagcaggc agacgtcgag gatgccgacg agcaggaaca ccacgcccag tacgccgagg
      661 acccacgccc acgcgccgcc cgccacgaag gcgaggacga ggaggatgac gcccagaccg
      721 gagcgcacat agcggcccgt ctgcgacgcg aggaagttga gaagtgcgga catgagtgcc
      781 tccaagagga ccggtggggt ttacctcccc agagtagtca cgatcgcacg gtactgggca
      841 cgctcgaacc cggcagggtc ggggaccgac gccgcggaca tactgccgcg cagttgcctc
      901 accgactcgt actcgttggc ctccagccac tcccggacct cggccagcat ggtcgtcacg
      961 tgacccggac ccctgcgcag cacggacgac gtcgtgcagg ccacgtccgc cccgacgagc
     1021 agcgccttga gcgcgtccgc ccccgtgtgg acgccggaac tggcggccag ggacgtctcg
     1081 ggcagttgcg gccgcaggat cccgagccag cggagcggca gccgcaggtc gtcgctctcc
     1141 gagagcgtga gcgcagggcg gaccgtgagc gtgtccaggt cgatgtcggg cgcgtagaac
     1201 cggttgaaca gcaccagccc gtccgcgccg cgtccgacca cggcggcggc gaagtgcgcg
     1261 accgacgtgt agaacggcga gagcttcacg gtgagcggca cggtgaccgc cgcctccgac
     1321 agcggggcac tcgcgaactc ggcgaacccc tcgccgtcgt cgaggaggtc cgcgaggtgg
     1381 agttcctcct cgatgacgtc ctcctcgaac agcgacggca ggactacggc ggaggcgccg
     1441 gagtcctcga gcgcgcgcag cccgtcgagc gtgccggtca gtggcccggc cgaggccatg
     1501 atcggccccg acaactccag cccgaggtaa cgcgtggtca ggtcggcgct cattctgttc
     1561 ccttctcgtc gtccgggccg gcgggggtgt caacctggac ggcgcgttcc acgccggcca
     1621 actggctgta gtagcgccac cgctcgtcga cgtcgcgctg ggcgaggcgg gcgagcatct
     1681 cagcgcgctg cgggttggac cgggccagca ttgagaaccg cgtctcggcc atcatgaagt
     1741 ctgtgaccgg cttggtgggc gccttgctgt ccaggctcat gggttgcgtg tgttcgtcgg
     1801 tgccggggcg gtaccggtac agcggccagt acccggacgc gacggcgtcg gtttgatgcg
     1861 tcatcgaggt ccccatgtcg atgccgtgcg cgatgcaggt ggagtaggcg atcaggaggg
     1921 agggccccgg ccaggcggcg gcctcgagca tcgcccggac ggtctgctgc tggttggcgc
     1981 cgatcgccag ttgtgccaca tacacgtcgc cgtacgcggc ggcgaccatg ccgaggtcct
     2041 tcttggggtg gtccttcccg gacgccgcga acttggccgc cgcgccgcgc ggggtggcct
     2101 tggacgcctg cccgcccgtg ttggagtaca cctcggtgtc aagcaccagg acgttcacgt
     2161 tccgccccga cccgaggacg tggtcgaggc cgccgaagcc gatgtcgtac gc
//
LOCUS       DASRKH010000314         2694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_1214, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000314 DASRKH010000000
VERSION     DASRKH010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1214"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VFC06_05035"
     CDS             <1..199
                     /locus_tag="VFC06_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HZL81292.1"
                     /translation="VPFSEVDEQFAAAEGEDDGSLAAWRSSHEEYFRRRATDAGGQPF
                     SEDLPVVLEEFELRYPDLSAH"
     gene            243..1310
                     /locus_tag="VFC06_05040"
     CDS             243..1310
                     /locus_tag="VFC06_05040"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HZL81293.1"
                     /translation="MVENSTARDIRLAVVAGDGIGTEVVEQGLRVLDAALVGTGVRVL
                     TTEFDLGARRWHATGETLTDTDLDAIRTHDAILLGAIGDPGVPSGVLERGLLLKLRFA
                     LDQYVNLRPSRLYPGVTSPLAHPGEIDFVVVREGTEGPYVGNGGAIRAGTPHEVANEV
                     SVNTAFGVERVVRDAFARAAARPRKKLTLVHKHNVLVYAGHLWRRTVDAVNAEFPDVT
                     VDYLHVDAATIFMVTNPSRFDVIVTDNLFGDIITDLAAAITGGIGLAASANINPDRTT
                     PSMFEPVHGSAPDIAGQGKADPTATVLSVALLLDHLRLPAAAAKVEAAVAADLVDRGA
                     GAARVRSTAEVGTDLAARVAD"
     gene            1375..2481
                     /locus_tag="VFC06_05045"
     CDS             1375..2481
                     /locus_tag="VFC06_05045"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HZL81294.1"
                     /translation="MSTTSSPATSTFEIRRSDAPVPAAARAEAMAAPKFGTVFTDHMA
                     RVSWSQADGWHDRRVEKYGPLLLDPATAVLHYAQEIFEGLKAYRHADGSVWTFRPSAN
                     AARFARSAHRLALPELSEADFLGAITALVATDVDWVPSGDETSLYLRPFMYGSERFLG
                     VRPSLEAEFLVIASPVGPYFTHGVQPVSIWVAQDYHRVGAGGTGAAKCGGNYAASLLP
                     QQEAYGHGCEQVCFLDAATNAVLEELGGMNVFIVHADGSVSTPELTGSILEGVTRSSV
                     IRLLTDAGHEMSERRITLAELRAGLTDGSVTEVFACGTAAVITPIGRLAGEGFDMTVA
                     DGAAGDVTMRVRAELTDIQYGRATDRRGWLHRLV"
BASE COUNT          395 a          976 c          926 g          397 t
ORIGIN      
        1 cgtgccgttc agcgaggtgg acgagcagtt cgctgcggct gagggcgagg acgacggttc
       61 actcgccgcg tggcggagca gccacgagga gtacttccgg cggcgcgcca cggacgccgg
      121 cggtcagccg ttcagcgagg acctgccggt cgtgctcgag gagttcgagc tgcgctatcc
      181 ggatctgtcc gcgcattgag attaccgtcc cgacccttgg gacgagcggt agcctttcga
      241 ccatggtcga gaacagcaca gcccgggaca tccgcctcgc cgtcgtcgcg ggtgacggca
      301 tcggcaccga ggtcgtcgag cagggcctgc gggttctcga cgccgcgctc gtcggcaccg
      361 gcgtgcgcgt gctcacgacc gagttcgacc tcggcgcgcg gcggtggcac gcgacggggg
      421 agaccctcac tgacaccgac ctcgacgcga tccgcacgca cgacgcaatc ctgctcgggg
      481 ccatcggcga cccgggcgtg ccgtcgggcg tgctcgagcg cgggctgctc ctgaagctgc
      541 gctttgcgct cgaccagtac gtgaacctgc gcccgagccg gctgtacccc ggcgtgacgt
      601 ccccgctcgc ccaccccggt gagatcgact tcgtcgtcgt ccgcgagggc accgaggggc
      661 cgtacgtcgg caacggcggc gcgatccgcg ccggtacccc gcacgaggtc gcgaacgagg
      721 tgagcgtcaa caccgcgttc ggcgtcgagc gcgtcgtgcg ggacgcgttc gcccgcgccg
      781 cggcccgccc gcgcaagaag ctcacgctcg tgcacaagca caacgtgctc gtgtacgccg
      841 gtcacctctg gcgccgtacg gtcgacgcgg tgaacgccga gttccccgac gtgaccgtcg
      901 actacctgca cgtggacgcc gcgacgatct tcatggtgac caacccgtcg cgcttcgacg
      961 tgatcgtcac cgacaacctg ttcggcgaca tcatcaccga cctcgccgca gcgatcacgg
     1021 gcggcatcgg tctcgccgcg tcggccaaca tcaacccgga ccgcaccaca ccgagcatgt
     1081 tcgagcccgt gcacggctcg gccccggaca tcgccggcca gggcaaggcc gacccgacgg
     1141 ccaccgtgct gtcggtcgcc ctgctgctcg accacctccg cctgcccgcg gccgctgcca
     1201 aggtcgaggc tgccgtcgcc gcggacctcg tggaccgtgg cgccggagcg gcccgagtac
     1261 ggtcgacggc cgaggtgggc accgatctcg cggcacgcgt cgccgactga ccccgcccct
     1321 cggccgtcct caccggtaac gtcagcatcc gaacctgctg aaaggcctcc gcacatgagc
     1381 acgacttctt cgcccgcgac atccaccttc gagatccgtc gcagcgatgc cccggtcccg
     1441 gccgccgcgc gcgccgaggc gatggcggca cccaagttcg gcacggtctt caccgaccac
     1501 atggcgcgcg tctcgtggtc gcaggccgac ggctggcacg accgtcgcgt cgagaagtac
     1561 ggcccgctcc tgctcgaccc ggcgaccgcg gtgctgcact acgcacagga gatcttcgaa
     1621 ggtctcaagg cgtaccgcca cgccgacggg tccgtgtgga cgttccggcc gtcggccaat
     1681 gccgcacggt tcgcgcgctc cgcgcaccgg ctcgcactgc ctgagctctc cgaggccgac
     1741 ttcctgggtg ccatcaccgc gctcgtcgcg accgacgtcg actgggtccc gtcgggcgac
     1801 gagacgagcc tgtacctgcg cccgttcatg tacgggtccg agcggttcct cggggtgcgg
     1861 ccctcgctag aggccgagtt cctcgtcatc gcgtcgccgg tcggcccgta cttcacgcac
     1921 ggcgtgcagc cggtgtcgat ctgggtcgcg caggactacc accgcgtggg tgcgggcggc
     1981 acgggtgcgg ccaagtgcgg gggcaactac gcggcgagcc tgctgcccca gcaggaggcg
     2041 tacgggcacg ggtgcgagca ggtgtgcttc ctcgacgctg cgacgaacgc ggtcctcgag
     2101 gagctcggcg ggatgaacgt gttcatcgtg cacgccgacg ggagcgtctc gacccccgag
     2161 ctgaccgggt cgatcctcga gggcgtgacg cgctcgtcgg tcatccggct gctcaccgac
     2221 gcgggtcacg agatgtccga gcgtcggatc acgctcgcgg agctgcgggc gggcctcacc
     2281 gacgggtcgg tcacggaggt gttcgcgtgc gggaccgcgg cggtcatcac cccgatcgga
     2341 cgcctcgcag gggagggctt cgacatgacc gtcgcggacg gcgcggcggg agacgtcacg
     2401 atgcgggtcc gtgccgagct gaccgacatc cagtacggcc gggcaaccga ccggcgcggg
     2461 tggctccacc gcctcgtctg acggcccgag cagctgacac acccatgtcg cgcggcccgt
     2521 ggacgagcgt gcaggctcgt ccgggccgtg tggcatgatg tggcctgtga cacaccttca
     2581 ctcgctccac ctcattatcg agtagcgcgc ctgcacatcg cagacgcgct actcgataat
     2641 gaggtggagc gagtgaaggt gtgtcacagg ccacatcatg ccacacggcc cgga
//
LOCUS       DASRKH010000315         2034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_25965, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000315 DASRKH010000000
VERSION     DASRKH010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25965"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="VFC06_05050"
     CDS             <1..913
                     /locus_tag="VFC06_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HZL81295.1"
                     /translation="YNRRSRFPERMPWVRLTHEPFPRLSIVREVRVDGGECGSTSGPV
                     SAHIGPFASQGAAQSAIDALHETFAIRQCAQRLPLVAAPGATACALAEMGRCAAPCTG
                     RQDPSAYADVVQDVRAAMLSDPSAVIGAHATRIAALVTQERFEEAGAVRDRLTAFLRG
                     AARAQRLAPLSACPELVAARRSPDGGWEVVLIRYARLAATATVDRTDDPWPVIESLRL
                     SAEHVERPVSPAPAAHPEEADLLLAWLDSPGVRLVDAPEGWTCPVRGAQSCAGTEATA
                     ASVSAYLAAAPPDDTGSRVRAHAAGAA"
     gene            921..1202
                     /locus_tag="VFC06_05055"
     CDS             921..1202
                     /locus_tag="VFC06_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HZL81296.1"
                     /translation="MLTAIVLIDTDAARIPEVAAAIAEVPGVSEVYSVTGEVDLIALV
                     RVREHDQLADVIADHLSKVEGVLRTQTYIAFRTYSKHDLDEAFSLGLED"
     gene            complement(1219..>2034)
                     /gene="trpD"
                     /locus_tag="VFC06_05060"
     CDS             complement(1219..>2034)
                     /gene="trpD"
                     /locus_tag="VFC06_05060"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HZL81297.1"
                     /translation="VDIVGTGGDRAHTVNISTMASLVIAGTGLRVVKHGGRASSSSTG
                     SADVLEALGIRLDNTVGRVAELATEVGITFCFAQVFHPSMRHAAVARRELGIATAFNF
                     LGPVTNPAQPAAAAIGVADARMAVLMASVLAGRGTSALVFRNDDGLDELAPTAPSTIW
                     EVRDGVVVEHRLDSVADLGLTALEVADLRGGESEFNAGVVRAVLAGELVPARETVLLN
                     AAAALVADGTLPGTAHRTLVERLRAGLELAAASVDSGAALDVLERWRAASAAS"
BASE COUNT          276 a          763 c          729 g          266 t
ORIGIN      
        1 gtacaaccgg cgctcacgct tccccgagcg catgccctgg gtgcgcctga cgcacgagcc
       61 gttcccgcgg ctctcgatcg tccgcgaggt ccgggtcgac ggcggtgagt gcgggagcac
      121 gagcggaccg gtctcggccc acatcggacc gttcgcgtcg cagggcgcgg cgcagtccgc
      181 gatcgacgcc ctgcacgaga cgttcgcgat ccgccagtgc gctcagcgcc tgcccctcgt
      241 cgcagctccc ggtgcaaccg cgtgcgcact cgccgagatg ggacgctgcg ccgcaccgtg
      301 caccggtcgc caggacccga gcgcgtacgc cgacgtcgtg caggacgtcc gggctgcgat
      361 gctctcggac ccgtcggccg tgatcggtgc ccacgcgaca cggatcgcag ctctcgtcac
      421 gcaggagcgc ttcgaggagg cgggcgctgt gcgggacagg ctgaccgcgt tcctgcgcgg
      481 tgccgcccgt gcgcagcggc tcgcaccgct ctccgcctgc cccgagctcg tcgcggcccg
      541 gcggtcaccc gacggcgggt gggaggtcgt gctcatccgg tacgcgcggc tcgcagccac
      601 agcgacggtc gaccgcaccg acgacccctg gcccgtgatc gagtcgctgc gcctgtccgc
      661 cgagcacgtc gagcgtccgg tctcccctgc gcccgctgca caccccgagg aggccgacct
      721 gctgctcgcg tggctggact cgcccggtgt acggctcgtc gacgcccctg aggggtggac
      781 gtgcccggtg cgcggtgcgc agtcgtgcgc cgggaccgag gcgacggccg catccgtctc
      841 ggcctacctc gcggccgcgc cacccgacga caccggatca cgcgtgcgcg cgcacgctgc
      901 gggagcggca tgatggccgc atgctgactg ccatcgtgct catcgacacc gacgccgccc
      961 gcatccccga ggtcgctgcg gcgatcgccg aggtgcccgg cgtgagcgag gtctactccg
     1021 tgaccggcga ggtcgatctc atcgccctcg tgcgggtccg cgagcacgac cagctcgccg
     1081 acgtcatcgc cgaccacctg agcaaggtcg agggcgtgct ccggacgcag acgtacatcg
     1141 cgttccggac gtactccaag cacgacctcg acgaggcctt ctcgctcggc ctcgaggact
     1201 gactcgagga ctgatcggtc aggacgcggc cgaggcggcc cgccaccgct cgaggacgtc
     1261 gagggcggca ccggagtcga ccgacgcggc ggccagctcc aggcccgctc gcagccgttc
     1321 gacgagcgtc ctgtgtgcgg tgcccggcag ggtcccgtcg gcgacgaggg ccgctgcggc
     1381 gttgagcagc acggtctcgc gcgccgggac gagctccccc gccagcaccg cgcggacgac
     1441 cccggcgttg aactccgact cgcccccgcg caggtccgcg acctcgaggg cggtgagccc
     1501 gaggtccgcg acggaatcca ggcggtgctc gaccacgact ccgtcacgca cctcccagat
     1561 cgtcgacgga gccgtgggtg cgagctcgtc gagaccgtcg tcgttgcgga agacgagcgc
     1621 gctcgtcccc cgccccgcca gcacgctcgc catcaggacc gccatgcgcg cgtcggcaac
     1681 accgatggcc gccgcggcgg gctgagcggg gttggtgacc gggccgagga agttgaacgc
     1741 cgtggcgatg ccgagctcgc ggcgggcgac ggcggcgtgc cgcatcgagg ggtggaagac
     1801 ctgcgcgaag cagaacgtga tgccgacctc ggtcgcgagc tcggcgaccc gaccgacggt
     1861 gttgtcgagc cggatgccga gcgcctcgag cacgtccgcc gacccggtgg acgacgacga
     1921 ggcccgcccg ccgtgcttga cgacgcgcag gcccgtaccc gcgatcacga gcgaggccat
     1981 cgtcgagatg ttcacggtgt gggcgcggtc gccacctgtg ccgacgatgt cgac
//
LOCUS       DASRKH010000316         2427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_45378, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000316 DASRKH010000000
VERSION     DASRKH010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45378"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            728..1105
                     /gene="rpsL"
                     /locus_tag="VFC06_05065"
     CDS             728..1105
                     /gene="rpsL"
                     /locus_tag="VFC06_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007930014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HZL81298.1"
                     /translation="MPTIQQLVRKGRSPKASKSKTPALKGSPQRRGVCTRVYTTTPKK
                     PNSALRKVARVRLSSGIEVTAYIPGEGHNLQEHSIVLVRGGRVKDLPGVRYKIVRGSL
                     DTQGVKNRKQARSRYGAKMEKKK"
     gene            1105..1575
                     /gene="rpsG"
                     /locus_tag="VFC06_05070"
     CDS             1105..1575
                     /gene="rpsG"
                     /locus_tag="VFC06_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HZL81299.1"
                     /translation="MPRKGPAPKRPIIIDPVFGAPVVTQLINKVLLDGKKATAESIVY
                     GALEGVKEKSGQDPVVLLKRALENVRPALEVRSRRVGGATYQVPVDVRPVRQTALALR
                     WLVDFSRKRRENTMTERLMNEILDASNGLGAAVKRREDMHKMAESNKAFAHYRW"
     gene            1624..>2427
                     /locus_tag="VFC06_05075"
     CDS             1624..>2427
                     /locus_tag="VFC06_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HZL81300.1"
                     /translation="MAQDVLTDLTKVRNFGIMAHIDAGKTTTTERILFYTGITYKIGE
                     VHDGAATMDWMAQEQERGITITSAATTCFWDNTQLNIIDTPGHVDFTVEVERNLRVLD
                     GAVAVFDSVAGVEPQSETVWRQADRYSVPRICFVNKMDKLGADFYFTVKTIVDRLKAK
                     PLVIQLPIGSESEFTGVVDLVEQKALIWSGETKLGEEYSTEEIPADLVEKAAQYRAEL
                     IEAVAEANETLLEKYLNGEELTVAEIKAGIRELTIKSLAYPVLCGSAFKN"
BASE COUNT          477 a          758 c          787 g          405 t
ORIGIN      
        1 ccgacgaacc atggccaccg agctctcgtt tgcgacgatc cagccccaga atgatcgggt
       61 ttgccggaat gtgcggtcgg ttcactcatg agatccccct cgttggtgcg tcgccggtgt
      121 gcgctcacct ttcgaaccga ccatacccaa tgtgtctccg ccgctcatca cgagcgacgg
      181 ggtccttcgt gcgttgtgcg gcgattcttc aacccgcaag agtgacacag gggacatgca
      241 attttctaaa gctgtcggcc cagcggccga tggggagggc aacaccttcg atgaggcgaa
      301 gagaggcccg acatggtcac gtttcttgcg tggtggaccg tagccgcagt gattgcgtgc
      361 tcaggaatca ccgcttgggt gatgcgccac aaccctcact ttcactaagg cgtgcggggg
      421 aggggtccgc tgagcgcgga gtggcgcgcg aaaagagcta cgtgtgaggg gccttgagag
      481 ggggctcgtt cgtcgtcgca gagtccggta tcatggacac ccgagggtgc atgctcgccg
      541 cgcccacggg atggagatca tccgctttga cggcaggcgt agcgccaggt atcgttgatc
      601 ctcgtgcccg cgtcgtcggg cccccacgcg cgccctactg ggcgtcacca agaaggtgcg
      661 acacgcccgg acgcgggggt cgggccgacg acatgacaag cgttcagttt caacagacgg
      721 agatgcagtg cctacgattc agcagttggt gcgcaagggg cgttcgccca aggcgtccaa
      781 gtccaagacg ccggccctta agggcagccc tcagcgccgt ggagtgtgca cgcgcgtgta
      841 tacgaccacg cccaagaagc cgaactcggc tctgcgcaag gtggcacgcg ttcgcctgtc
      901 ctcgggcatt gaggtcacgg catacatccc cggcgagggc cacaacttgc aggagcactc
      961 gatcgtgctc gtgcgtggcg gtcgtgtgaa ggaccttccc ggcgtgcgtt acaagatcgt
     1021 gcgcggctcg ctcgacaccc agggcgtgaa gaaccgcaag caggctcgca gccgttacgg
     1081 cgcgaagatg gagaagaaga agtaatgcct cgcaaaggac cggcgcctaa gcgccccatc
     1141 atcattgacc cggtgttcgg cgctcccgtc gtgacccagc tgatcaacaa ggtgctgctc
     1201 gacggcaaga aggcgaccgc cgagtcgatc gtgtacggcg ccctcgaagg cgtcaaggag
     1261 aagagcggcc aggaccccgt cgtcctgctg aagcgtgcgc tcgaaaatgt ccgccccgcc
     1321 cttgaggtcc gctcccgccg cgtcggcggt gcgacctacc aggtccccgt ggacgtgcgt
     1381 cccgtgcgtc agaccgccct tgctctgcgc tggttggtgg acttctcgcg caagcgtcgc
     1441 gagaacacca tgaccgagcg cctcatgaac gagatcctcg acgcgtccaa cggcctcggt
     1501 gccgcagtga agcgtcgcga agacatgcac aagatggccg agtcgaacaa ggcgttcgcg
     1561 cactaccgct ggtagttcgt aagccaagtt cgtcgacaga acacacttaa gggagaacca
     1621 ctcgtggcac aggacgtgct gacggacctc acgaaggtcc gcaacttcgg catcatggct
     1681 cacatcgatg ccggcaagac gaccaccacc gagcgaatcc tgttctacac gggcatcacc
     1741 tacaagatcg gtgaagtgca cgatggtgcg gccacgatgg actggatggc acaggagcag
     1801 gagcgtggca ttaccatcac ctccgctgcg acgacgtgct tctgggacaa cacccagctg
     1861 aacatcatcg acacgcccgg tcacgttgac ttcaccgtcg aggtggagcg caacctgcgc
     1921 gtgctcgacg gcgcggtcgc ggtgttcgac tcggtcgcag gcgtcgagcc gcagtccgag
     1981 acggtgtggc gccaggccga ccgctacagc gttccccgca tctgcttcgt gaacaagatg
     2041 gacaagctcg gcgcggactt ctacttcacg gtgaagacga tcgtcgaccg cctcaaggcc
     2101 aagccgctgg tcatccagct gccgatcggc tcggagagcg agttcacggg cgtcgtcgac
     2161 ctggtcgagc agaaggcgct catctggagc ggcgagacca agctgggcga ggagtactcc
     2221 acggaggaga tccccgcgga cctcgtcgag aaggcggcgc agtaccgcgc cgagctcatc
     2281 gaggccgtcg ccgaggcgaa cgagactctt ctcgagaagt acctgaacgg cgaagagctc
     2341 acggtcgcgg agatcaaggc cgggatccgg gagctgacga tcaagtcgct cgcgtacccg
     2401 gtcctgtgcg ggtcggcgtt caagaac
//
LOCUS       DASRKH010000317         2531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_19333, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000317 DASRKH010000000
VERSION     DASRKH010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19333"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VFC06_05080"
     CDS             complement(<1..491)
                     /locus_tag="VFC06_05080"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZL81301.1"
                     /translation="MSQSRSKLGFNATWSMAVGGMVGGGIFSTLGVVIGIAGPWAWLS
                     FVTAGLIALAAGYSYVRLATHYGEGGGAFTFLREIDAEGFAGSLSWVLIIGYVLTNAV
                     YAFTFGQYLGHVVGLGAWFPRLAGVVIVALFIGLNLRRVGEAGGVEVILVWFKLAALV
                     ALAG"
     gene            complement(995..1171)
                     /locus_tag="VFC06_05085"
     CDS             complement(995..1171)
                     /locus_tag="VFC06_05085"
                     /inference="COORDINATES: protein motif:HMM:NF012691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81302.1"
                     /translation="MTPDAGRQPAQADAYGVMEPCTLVLFGASGDLAQRMVVPAIFRL
                     ARRGLLIADAIPSA"
     gene            complement(1168..>2531)
                     /locus_tag="VFC06_05090"
     CDS             complement(1168..>2531)
                     /locus_tag="VFC06_05090"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HZL81303.1"
                     /translation="DDKAVQIHDEAMATAGMVMAHPGAHWAHFANMTLGLWLITGIFA
                     LGYRSTALQASDAVSGALVIVLAALSLSHRPWFKFWAPWANSLVGLWLLFAPLVFWAP
                     TATAYANDTLVGALVVVFAILAPGMPMAPGTSMETGPDVPRGWSYNPSSWPQRAPIIA
                     LALVGLFLSRQMAAFQLQHITSFTDPFFGLGTERVLTSDVSRAFPIPDAGLGAVAYMI
                     EFLMGFMGDKARWRTMPWMVTFFGILVVPLGVVSITLVILQPIAVGSWSTPALIAAAA
                     MLTMIALTLDEVAAMLQFLVQARSEGQPLWRIFWQGGILRTVPETTHVTARPDMVRPM
                     AMFWGVSLPWNLIVSAALGLWLMFAPSVLGSSGMAAHSDHLVGALTLTVAVIVLAEVG
                     RAMRFVNVLLGAWIVVSPWLLGGATTAATWSDMIAGVLIIVLSFPRGPVGERYGSVEG
                     LIR"
BASE COUNT          511 a          854 c          800 g          366 t
ORIGIN      
        1 cccgccagcg ccaccagcgc cgccagcttg aaccagacga ggatcacctc gacgccgccg
       61 gcctcgccga cgcgtcgcag gttcaaccct atgaacagag ccacgatgac gacgcctgcc
      121 aggcgcggga accacgcgcc cagccccacg acgtgcccga ggtactgccc gaacgtgaac
      181 gcgtacaccg cattcgtaag cacgtacccg atgatgagca cccaggagag gctgccggcg
      241 aacccctccg cgtcgatctc gcggaggaat gtgaaggcgc cgcccccttc gccgtagtgg
      301 gtggccagtc ttacgtagct gtagcccgcg gccagcgcga tgaggccggc ggtcacgaag
      361 ctgagccatg cccacggacc ggcgatcccg atgactaccc cgagtgtcga gaagatgccg
      421 ccgccgacca tgccgccgac ggccatcgac cacgtcgcgt tgaagccgag cttggagcgc
      481 gactgactca catcaagagt ctcccaggcg gcaaagttcg gtatgacggc agagctgagc
      541 cgccgaccta ctgcgccctg agccacagcc ggcgaggcgc aacgacctca cgaaccaccg
      601 ccgaccggat acgacaggtg aacaagatac gtcacggcgg gccttcctcg ttgaccagag
      661 cctctgagtc gcgccgcggc agtcggtcgg ctccagcgac cggctggcgg ctccaactcc
      721 aagcttcttg atctgctcgt ctttctggct tcctcatccc ggggccgtgt gcgaagcgca
      781 ttctctgcgg tcagggggaa tttctcccgc cagccctcga ccttggccac ggtgttcgtc
      841 aacttcacct cttcctcgcc cagcggcgag ccgtgatcgg caaaggtatc ctgcctgtgc
      901 ggtgaaccgt aaccaacgtg cgtccgcacc aagagcagcg ggggaaggtc ggtttcctgg
      961 cgagcagcgc ggatggcacg gtcgatagcc tggattatgc cgaaggaatc gcgtccgcaa
     1021 taagtagacc gcgccgcgcg aggcgaaaga tggcgggcac caccatgcgt tgcgccagat
     1081 cgcccgaagc accgaacagc accagcgtgc acggttccat cacaccgtat gcgtcggcct
     1141 gtgccggctg tctcccagca tcaggggtca tcgtatgagt ccttcaacgc taccgtaccg
     1201 ctcacccacc ggaccgcggg ggaaactgag gacgatgata agcacgccgg cgatcatgtc
     1261 gctccaagtc gcggcggtcg tggcaccgcc cagcagccat ggggacacaa ctatccatgc
     1321 gcccaacaac acattcacaa agcgcatcgc gcgacccacc tcagcaagca cgatcacggc
     1381 aacggtcaaa gtcagggccc ccacgagatg gtcgctgtga gcggccatcc cgctgctgcc
     1441 caagacggac ggcgcgaaca tcagccagag tcccagcgcc gcgctgacga tcaggttcca
     1501 cggcagtgac actccccaga acatcgccat cggacgcacc atgtcggggc gggccgtaac
     1561 gtgtgtggtc tcgggcacag tccgcagtat gccgccctgc cagaatatcc gccacaacgg
     1621 ttgcccctcg ctcctcgctt gaacgaggaa ctggagcatt gccgccacct cgtcgagagt
     1681 gagggcgatc atggtcagca tcgccgcggc ggcgatgagg gcgggcgtgg accacgagcc
     1741 gacggcgatc ggctgcagga tgacgagggt gatgctgacg acgccgagcg gcacgacgag
     1801 gatgccaaag aacgtgacca tccacggcat cgtgcgccag cgagccttgt cgcccatgaa
     1861 gcccatcaga aactcgatca tgtaggcgac cgcacctagt ccggcatcgg ggatcggaaa
     1921 ggcgcgcgaa acgtcggagg tgaggacgcg ctccgtgcca agcccgaaga atggatcggt
     1981 gaacgacgtg atgtgctgaa gttggaacgc ggccatctgc cgcgagagga agaggccgac
     2041 gagcgcgagt gcgatgatcg gcgcccgctg cggccagctg gaggggttgt acgaccagcc
     2101 acgcggtacg tccggaccgg tctccatgct cgttcccggc gccatcggca tccccggcgc
     2161 caggatggcg aacacgacga ccaacgcgcc gaccaacgtg tcgttggcgt aggcggtggc
     2221 cgtgggcgcc caaaagacga ggggggcgaa gagcagccac agaccgacga gtgagttcgc
     2281 ccatggggcc cagaacttga accacgggcg gtgcgacaac gagagggcag cgagaacgat
     2341 gaccagcgcg ccgctcacag cgtcgctcgc ctgcagcgcg gtgctgcggt atcccagggc
     2401 gaagatgccc gtaatcaacc acaagccgag cgtcatattg gcgaagtgcg cccagtgcgc
     2461 tcccgggtgt gccatcacca tgccggctgt cgccatcgcc tcgtcgtgta tctgcaccgc
     2521 tttgtcatca t
//
LOCUS       DASRKH010000318         2186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_19347, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000318 DASRKH010000000
VERSION     DASRKH010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19347"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /locus_tag="VFC06_05095"
     CDS             complement(<1..1025)
                     /locus_tag="VFC06_05095"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HZL81304.1"
                     /translation="MLALGGLGEVGRNMHVIEYNGRLLIIDCGVLFPEENQPGVDLVL
                     PDLSYLEDRIDDIEAIVLTHGHEDHIGAVPFLLRMRRDIPILGSQLTLAFLEAKLVEH
                     RIKPLTLGVKEGQREKIKDFDLEFVAVNHSIPDALAVFVRTPAGTILNTGDFKMDQLP
                     LDGRITDLRAFARFGEEGVDLFQVDSTNAEVPGSTTSEVEIAPTLDRLFANAEGRVIV
                     ASFASHVHRVQQVIDAAHNHGRRVAFVGRSMVRNMGIAAELGFLKVPDGILIDPKKAD
                     QLPPEKVVFMSTGSQGEPMAALSRMANKDHKVQVTPNDLVILSSSLIPGNENAVFRVI
                     NGLMRIGA"
     gene            complement(1079..1963)
                     /gene="dapA"
                     /locus_tag="VFC06_05100"
     CDS             complement(1079..1963)
                     /gene="dapA"
                     /locus_tag="VFC06_05100"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HZL81305.1"
                     /translation="MTKPFGTVLTAMVTPMHEGGAIDLAASQRLAKYLVSRGSDGLVL
                     SGTTGEAPTTHAPEKVDLLRAVRDAVGPDVTLLTGAGSNDTAHAVRMAEQGSENGADG
                     ILALTPYYSKPTQAGIVAHFRAIVGATALPVLVYDIPGRTGVAIGEWAYDELAALERI
                     VAIKDATGDVKSAKARIERTGLAWYSGDDALTLDFLRAGAVGVVSVSSHIASREIAAM
                     IAAFEAGDTAEAQRIHEALLPVHAAIFNGPGATNAKSAMHWAGVISSRAMRLPLLPAS
                     DAEYVALTAALEAAGMTR"
BASE COUNT          359 a          687 c          755 g          385 t
ORIGIN      
        1 gcgccgatgc gcatcagccc gttgatgacg cggaacaccg cgttctcgtt tccgggaatc
       61 agggacgaag ataggattac caggtcgttc ggagtcacct ggaccttgtg gtccttgttg
      121 gccatgcggc tcagcgcggc catcggctcg ccctgcgaac cggtggacat gaagacgacc
      181 ttctccgggg gcagctggtc ggccttcttg gggtcaatga ggatgccgtc ggggaccttg
      241 aggaacccga gctcggcagc gatgcccatg ttgcgcacca tcgagcgccc cacaaatgcg
      301 acacggcggc cgtggttgtg ggcggcgtcg atcacttgct ggacgcggtg cacgtgactt
      361 gcgaacgaag cgacgatgac gcggccctcg gcgttggcga agaggcgatc cagcgtcggc
      421 gcgatctcca cctcggacgt cgtggatccg ggaacctcgg cgttggtgga gtccacctgg
      481 aacaggtcga caccctcctc gccaaagcgt gcgaaggcgc gcaggtccgt gatgcggccg
      541 tcgagtggca gctgatccat cttgaagtcg ccggtgttga ggatggtgcc ggcgggggtg
      601 cgcacaaaca cggccagtgc gtcggggatg gagtggttga cggccacgaa ctcgaggtcg
      661 aagtccttga tcttctcccg ctggccctcc ttcacgccga gcgtgagggg cttgatgcgg
      721 tgctcaacga gcttcgcctc aaggaacgcg agagtgagct gggaaccgag gattgggatg
      781 tctctacgca tgcgcagcag gaacggcact gcgccaatgt ggtcctcgtg accgtgcgtg
      841 agcacgattg cttcgatgtc gtcgatgcgg tcctcgaggt agctgaggtc cggtagcacc
      901 aggtcgacgc caggctggtt ctcttctggg aagagcacgc cacagtcgat gatgagcagg
      961 cgaccgttgt attcgatcac gtgcatattg cggcccacct cgcccagtcc cccgagcgca
     1021 agcatgcgca gggacccttc ggctagcggt ggcggggtgg gaagttcagg gtgaaagttc
     1081 atcgggtcat tcctgcggcc tccagggccg cggttagtgc aacgtactcg gcgtcgcttg
     1141 caggcaggag cggcaggcgc atggctctgc tcgaaatcac tccggcccag tgcatggcgg
     1201 acttggcatt ggtagcgcca ggcccattga aaatggcggc atgcacgggc aacagggcct
     1261 cgtgaatgcg ttgtgcttcg gcggtgtcgc ccgcctcgaa ggcggcgatc atcgccgcga
     1321 tctcgcgcga tgcgatgtgc gaggaaacgg aaaccacgcc caccgcccct gcgcgcaaga
     1381 agtcaagagt gagggcgtcg tcgcccgaat accaggcgag cccggtgcgc tcgatgcgcg
     1441 ccttggcgct cttgacgtcg cccgtcgcgt ccttgatggc gacgatgcgc tcaagggccg
     1501 cgagttcgtc gtacgcccac tcgcctatcg cgacgccggt gcggccgggg atgtcataga
     1561 ccaggacggg aagcgccgtg gcgcccacga tcgcccgaaa gtgagcaaca attcccgctt
     1621 gagtcggctt ggagtagtac ggcgtcagcg cgaggatgcc gtccgcgccg ttctccgagc
     1681 cctgctctgc catgcgcacg gcgtgggccg tgtcgttgga gcccgcaccc gtgaggagcg
     1741 tgacgtcggg accgaccgcg tcgcgcacgg cgcgcagcag gtcgaccttc tcgggagcgt
     1801 gcgtggtggg cgcctccccc gtggtcccgg aaagcaccag cccgtcactg ccgcgtgaca
     1861 ccaggtactt ggccagccgc tgggacgcgg caaggtcgat ggcgccgcct tcgtgcatcg
     1921 gcgtcaccat ggccgtcagc acggtgccaa agggcttggt catgggcact aacctaccgc
     1981 gtcggcgtgt cgcttcgttg tgcctgcgag cctcgcggcc cccgtctgcc gctccggtct
     2041 cgcgggcacc gcgggcgggc gccagtccgg cgtgtcgacc cgaccgcgtg aacaactcac
     2101 tagagagccg acaccgacgt ctgccagcga gcgtgcgacg aagcccgtag gtcactttgg
     2161 tagtgacttc ttcacgctcg ccactc
//
LOCUS       DASRKH010000319         2473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_14953, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000319 DASRKH010000000
VERSION     DASRKH010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14953"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VFC06_05105"
     CDS             <1..201
                     /locus_tag="VFC06_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81306.1"
                     /translation="VVTPPIATVNGTPEATIVDLLPRYAAWGLLEAVITGAAVAAGLA
                     WNRSVVRIEVAAEVRRRATVSS"
     gene            complement(244..669)
                     /locus_tag="VFC06_05110"
     CDS             complement(244..669)
                     /locus_tag="VFC06_05110"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZL81307.1"
                     /translation="MARHARGSHGGGFTRTFVVTEGGSPDVVAYYAWTMAGLGFGEAP
                     ARVSAGGGRHPVPMALLARLAVDQRHEGRGLGRALLRDVVSRTLEVGTRIGCRGLLVH
                     CETADARAFYLRHLPSLEASPIDPMHLVLLMKDLRRSVG"
     gene            complement(796..1080)
                     /locus_tag="VFC06_05115"
     CDS             complement(796..1080)
                     /locus_tag="VFC06_05115"
                     /inference="COORDINATES: protein motif:HMM:NF020265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HZL81308.1"
                     /translation="MAASTAKKDQRLEFRTTSENRELFARAAEASGTDLSGFANEQLR
                     VAALRVLADRTTFVLSPVEAALWDELMERPARSLPDVRALLAEPSIFVDE"
     gene            1270..1854
                     /locus_tag="VFC06_05120"
     CDS             1270..1854
                     /locus_tag="VFC06_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81309.1"
                     /translation="MGVESTRNDIERLRADFDRQPRESAIHLAQRLSWYSMQLALDKK
                     HAESEATWVEALRIAEHVLTDPGPSQFERTELIRIGAGTARCMVEAGRPEQALDVLAK
                     AEDQYQRLAADATDPNTVLALRGTILTGLANAHRALGDTDAELDALCEAALELVAGSG
                     TNPLIVRSMLQPPLNELHRLLSQDDAASDDAAQG"
     gene            complement(1973..2122)
                     /locus_tag="VFC06_05125"
     CDS             complement(1973..2122)
                     /locus_tag="VFC06_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81310.1"
                     /translation="MDTPADAAVSSSAVSDAHVADAPAEATDGPAPKAKPKRAPRKKP
                     TDTTE"
BASE COUNT          413 a          867 c          817 g          376 t
ORIGIN      
        1 gtggtcacgc cgccgatcgc caccgtgaac ggcactcccg aggcgaccat cgttgacctg
       61 ttgccgcggt atgccgcgtg gggcctgctg gaggcggtca tcacgggtgc ggccgtggcc
      121 gcaggcctgg cctggaaccg gtcggtggtg cggatcgaag ttgcggcgga ggtgcgtcgg
      181 cgcgcgaccg tctcgtccta gtcggcctcg acttgagtac gtgagggggg agagacccac
      241 cgcctatccg accgaccgcc tcaaatcctt catcagcaac accaggtgca tcgggtcgat
      301 cgggctcgcc tcgagcgagg gcaggtggcg caggtagaac gcccgcgcat cggcggtctc
      361 gcaatgcacc aggaggcccc ggcagccgat gcgcgtgccc acctccagcg tgcggctcac
      421 cacgtcgcgc aacagcgccc tgcccaatcc gcggccctcg tgccgttggt caacggcgag
      481 tctcgcgagc agcgccatcg gcacgggatg gcggccacct ccggccgaca ccctggccgg
      541 tgcctccccg aaacccagcc ccgccatcgt ccacgcgtag tacgccacca cgtcggggct
      601 gccgccctcc gtcaccacaa aggtgcgcgt gaagcctccc ccatgtgatc cgcgcgcgtg
      661 cctggccaac cactcagcct gctcagccga gcgagatcga aacccgtcaa tcacgtggtc
      721 cggtcggagc agggcggggg ccacatacgc tggagcgccc tcggcgccgg cgctcggcgc
      781 ggtcatccga gcggcctact cgtcgacgaa gatgctgggc tctgcaagca atgcccgaac
      841 atcgggcagg ctccgcgccg gccgctccat gagttcgtcc catagcgcgg cctccaccgg
      901 ggagagcaca aaggtggtgc ggtcggcgag cacgcggaga gccgcgacgc gcaactgctc
      961 attggcgaag ccgctgaggt cagtgcccga tgcctcggcc gcgcgggcga acagctcgcg
     1021 gttctccgaa gtcgtgcgga actcaaggcg ctgatccttc ttcgcggttg acgcagccat
     1081 gcgggctccc ttcccttccc gagtgtacgg acagtgtacg gaacgaatgg agaatgcgtc
     1141 aacgggaccg ggccctcggc acgccacccc agttccaaaa accccataac cggacaacgc
     1201 gcccatcccc cggagggcaa tcaagatcac ccctatgcgg tgtgacatcc ttcacataca
     1261 ctcacgaaga tgggcgtcga aagcactcgc aatgacatcg agcgactgcg cgccgacttc
     1321 gatcgccagc cccgcgagag cgccattcac ttggcccagc ggctgtcctg gtattcgatg
     1381 caacttgccc tggacaagaa gcacgccgag tccgaggcca cctgggtgga ggccctgagg
     1441 atcgcggagc acgtactcac cgaccccggt ccctcgcagt ttgaacgcac cgaactgatc
     1501 cgcatcggcg cgggcaccgc ccgctgcatg gtggaggccg gccggcccga gcaggcgctc
     1561 gacgttctgg ccaaggctga ggaccaatac cagcggctcg ccgccgacgc taccgacccc
     1621 aacaccgtgc tcgcgttgcg cggcacgatc ctcaccggcc tggcgaacgc tcaccgggcg
     1681 ttgggcgaca ccgatgcgga gttggacgcc ctgtgcgaag ccgccttgga actcgtagcg
     1741 ggctcgggca ccaacccgct cattgtgcga tcgatgctcc aaccgccgtt gaacgaactc
     1801 caccgcctgc tgtcgcagga cgacgccgct tccgacgacg cagcgcaggg ctagctcacc
     1861 aggctagccg cgagcgccgc attccgcgcg tcgtcgccct cccctcaaga caaagaagac
     1921 ccccaaccac ggggtcacca tggtcggggg tcgcggagag ggggtgaggg ctctactcgg
     1981 tggtgtcggt cggcttcttg cgcggcgcgc gcttgggctt cgccttgggc gccgggccgt
     2041 ccgtcgcctc ggcaggcgcg tcggcaacgt gcgcgtcgga aactgcgctg ctagaaaccg
     2101 cggcgtcggc tggggtgtcc acggctgcgc cggcgagctg cgctgctcgg agcccctggg
     2161 ccgaaactca ggcaactgtg agacaactct tggccccctc gccgttgcca tgttcaccac
     2221 ttcgcaactt cccgacccca accttttcgg cgcggtgtgc gttactaggt atgtagcgtg
     2281 cggtggcgcg tgcaggcgaa tccgagttgc acaacccgca tgggtcaacc agcatggaca
     2341 tcgggggact gtggccctcg cagtgcggcg cgcgtgcgct ctgcgaagac gacggtcgtg
     2401 gtctgggagg gcaagtagcg gtgcaagcaa gggactacgt ggtgttgcga gccgtcgagg
     2461 tggcgctcgc ggc
//
LOCUS       DASRKH010000320         2572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_55410, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000320 DASRKH010000000
VERSION     DASRKH010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55410"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="VFC06_05130"
     CDS             <1..434
                     /locus_tag="VFC06_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZL81311.1"
                     /translation="IVMLAVPGDEIALDRAVALGARGYLAPDVGRAELAAVAAHVLAS
                     PVVDDTERVTARVDPGPVVGRPLVEVPAQQSAREELALTKREVEVLVGMSNGRSNSQI
                     GADLFLSEDTVKTHARRLFRKLGAADRAQAVAIGLRRGLIR"
     gene            465..1994
                     /gene="guaB"
                     /locus_tag="VFC06_05135"
     CDS             465..1994
                     /gene="guaB"
                     /locus_tag="VFC06_05135"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018154963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HZL81312.1"
                     /translation="MAFGHPDSPARASDPFGYVGLTYDDVLLLPGETDVIPSEVDTTT
                     RLTREISVAIPLVSAAMDTVTESRMAIAMARQGGVGILHRNLSIDDQAHQVDLVKRSE
                     SGMITDPVTVGPEATLAELDDLCGKYRVSGLPVVDARGRLLGIITNRDLRFVAASDYP
                     VLRVREVMTAMPLVTAPVGIAREDAAALLARHKVEKLPLVDGDGMLRGLITVKDFVKS
                     EQYPMATKDSEGRLVVGAAVGFFGDAWERALALVDAGVDVLVVDTANGHARLMLDMVR
                     RLKSDPATRDVQIIGGNIATTDGAQALVDAGVDAVKVGVGPGSICTTRVVAGVGVPQI
                     SAIYAASLACKPAGVPVIGDGGLQYSGDIAKALVAGADSVMLGGLLAGCEESPGDLVF
                     VNGKQYKRYRGMASLGAMQSRGDRRSYSKDRYFQGDLSDDEIITEGIEGQVAYRGPLA
                     AVAHQLVGGLHQSMFYVGARTVPALQERGKFVRITPAGLKESHPHDVQMISDSPNYSS
                     R"
     gene            complement(2023..>2572)
                     /locus_tag="VFC06_05140"
     CDS             complement(2023..>2572)
                     /locus_tag="VFC06_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HZL81313.1"
                     /translation="GVTTAHARAHGAAPAVALEEIATQVAAAFSEGIPVVAFNACYDL
                     SILDAELTRHGLATIPDRLGRAAAPVLDPLVLDRHEDRYRRGKRKLVDLCGVYEVGTG
                     SLHSADVDVVATLDVLARMVERFPDLAELDLAALHARQVTAHRAWAEGFNAWRTGQGL
                     TGPGAEADWPTRAPAAAVPASR"
BASE COUNT          349 a          925 c          935 g          363 t
ORIGIN      
        1 cgatcgtcat gctggccgtc cccggcgacg agatcgcgct cgaccgggcc gtcgcgctcg
       61 gtgcccgggg ctacctggcg cctgacgtcg gacgcgccga gctcgccgca gtcgcggctc
      121 atgtgctcgc gagccccgtc gtggacgaca ccgagcgcgt cacggcacgc gtcgaccccg
      181 gccccgtcgt gggccgaccg ctcgtcgagg tgccggccca gcagtcggcg cgcgaggagc
      241 tcgccctcac caagcgcgag gtcgaggtgc tcgtcggcat gagcaacggg cgctcgaact
      301 cccagatcgg cgcggacctg ttcctgtccg aggacaccgt caagacccac gcccggcggc
      361 tgttccggaa gctcggcgcg gccgaccgtg cgcaggccgt cgcgatcggg ttgcgccgcg
      421 gactcatccg ctgacggtcc ggggcgccgt cgtatcatcg gcgcatggcc ttcggacacc
      481 ctgactcacc cgctcgagct tcggacccct tcggctacgt cggcctgacg tacgacgacg
      541 tgctgctgct cccgggcgag acggatgtca tcccgagcga ggtcgacacg accacgcgcc
      601 tgacgcgcga gatctccgtc gcgatcccgc tggtctccgc ggcgatggac accgtcaccg
      661 agtcacgcat ggccatcgcg atggcccgcc agggcggcgt cggcatcctg caccgcaacc
      721 tgtcgatcga cgaccaggcg caccaggtcg acctcgtcaa gcggtccgag tcgggcatga
      781 tcacggaccc ggtcaccgtc ggtcccgagg cgacgctcgc cgagctcgat gatctgtgcg
      841 gcaagtaccg ggtgtccggg ctcccggtcg tcgacgcccg gggtcgcctc ctcggcatca
      901 tcacgaaccg cgacctgcgg ttcgtcgccg cgagcgacta cccggtgctc cgcgtgcgcg
      961 aggtgatgac ggccatgccg ctcgtcacgg ctcccgtggg gatcgcccgg gaggacgccg
     1021 cggcgctgct cgccaggcac aaggtcgaga agctgccgct cgtcgacggt gacggcatgc
     1081 tgcgcgggct catcaccgtc aaggacttcg tgaagtccga gcagtacccg atggcgacga
     1141 aggacagcga gggtcgtctc gtggtcgggg cggccgtcgg gttcttcggt gacgcgtggg
     1201 agcgtgcgct ggccctggtc gacgccggcg tggacgtgct ggtcgtcgac accgcgaacg
     1261 gccacgcgcg cctcatgctc gacatggtcc gtcgcctcaa gtccgacccg gcgacccggg
     1321 acgtgcagat catcggcggc aacatcgcga ccaccgacgg cgcgcaggcg ctcgtcgatg
     1381 cgggcgttga cgcggtgaag gtcggcgtcg ggcccggctc gatctgcacg acgcgcgtcg
     1441 tcgcgggcgt cggggtcccg cagatctccg cgatctatgc ggcctcgctc gcgtgcaagc
     1501 ccgccggggt ccccgtcatc ggcgacggcg gcctgcagta ctcgggggac atcgccaagg
     1561 cactcgtcgc gggagcggac tccgtgatgc tcggcgggct gctcgccggc tgcgaggagt
     1621 cgcccggtga cctcgtgttc gtcaacggca agcagtacaa gcgctaccgc ggcatggcgt
     1681 ccctcggggc catgcagtcg cgcggcgacc gtcgctcgta ctccaaggac cggtacttcc
     1741 agggcgacct gtccgacgac gagatcatca ccgagggcat cgagggccag gtcgcgtacc
     1801 ggggccccct cgcggccgtc gcgcaccagc tcgtcggtgg cctgcaccag tcgatgttct
     1861 acgtcggggc gcggaccgtc ccggcgctgc aggagcgcgg caagttcgtg cggatcacgc
     1921 ccgcggggct caaggagtcc cacccgcacg acgtgcagat gatctccgac tcacccaact
     1981 actcgtcccg ctaggccgcg gcgcccccgg aggggcggtc ggtcagcggg acgcggggac
     2041 ggcggcggcc ggcgcgcgcg tcggccagtc agcctcggca ccgggtccgg tcagaccctg
     2101 gccggtgcgc caggcgttga acccctcggc ccacgcgcgg tgggcggtca cctggcgtgc
     2161 gtgcagcgca gcgaggtcga gctcggcgag gtccgggaac cgctcgacca tgcgcgcgag
     2221 cacatcgagc gtggcgacga cgtcgacgtc ggccgagtgc agcgacccgg tacccacctc
     2281 gtacacgccg cacaggtcga cgagcttgcg cttgccgcgg cggtagcggt cctcgtgccg
     2341 gtcgagcacg agcgggtcga ggaccggcgc ggcggcgcgg ccgaggcggt ccgggatcgt
     2401 cgcgaggccg tgccgggtca gctcggcgtc gaggatggac aggtcgtagc acgcgttgaa
     2461 ggccacgacc gggattcctt cggagaacgc ggccgcgacc tgcgtggcga tctcctcgag
     2521 cgcgaccgct ggcgccgcgc cgtgtgcgcg cgcgtgcgcc gtcgtgacgc cg
//
LOCUS       DASRKH010000321         2317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_51352, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000321 DASRKH010000000
VERSION     DASRKH010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51352"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1630)
                     /locus_tag="VFC06_05145"
     CDS             complement(<1..1630)
                     /locus_tag="VFC06_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013115615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZL81314.1"
                     /translation="MKLVIVGGVAGGMSAAARARRLNEAAEIIVLDRGPHVSFANCGL
                     PYHLSGEIPERQSLLLHTPATLKSQLNLDVRVNSEATAIDRAARTVTVSGPRGDYTLD
                     YDALILAPGARAMVPPIRGVDNPAVFPLRTIPELDAIMAHLAGLEGGHGPGEQLDEGA
                     PLRAVVVGAGFIGLEAVEALAHRGLEVTLVEFAPHVLPPLDDELAPLLQAELERNGVT
                     VRVGTSVVAIDDAPATGVVATLSDGTVVPADVVVVATGVVPDTALAKRAGLDLGFRDA
                     IAVDGAMRTSDAYIWAVGDAVQVRQRVGDVEVLGPVPLAAPANRHGRLAADAIMRGRA
                     ADGEPAPVVLGTAIVRVFGMIAAITGASQRMLRTNGVESTTVHIHPGSHAGYYPGSEQ
                     IHLIGSFAPDGTLLGAQAVGRDGVDKRIDVLATAIKAGMTADDLADLDLAYAPPFGSA
                     KDPVNMLAFTAQNILDGTMPVWYAQDLQWAKQNSLILDVRSAAECSRGKVFEALNVPH
                     TQVRDRIDEIRVAAAGRPIAVHCASGVRSYLATRI"
     gene            complement(1642..1899)
                     /locus_tag="VFC06_05150"
     CDS             complement(1642..1899)
                     /locus_tag="VFC06_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019484296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HZL81315.1"
                     /translation="MQLTGQDLVSVRNRLSRANGQLAAVIRLLDEGGDCENIVTQLAA
                     CSKALDKAGFAIIASSLRQCMEDPSEAMDPKRLEKLFLSLS"
     gene            complement(1911..>2317)
                     /locus_tag="VFC06_05155"
     CDS             complement(1911..>2317)
                     /locus_tag="VFC06_05155"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZL81316.1"
                     /translation="VVYPRGYLLCLSHLRTRRNMGLFDFLKRTPLDPTNLPAAVKAEE
                     AVALVTDGAVLVDVRENHEWKAGHAREAIHIPLARLASSTNRFKGRPVVVVCASGSRS
                     RQGASELRVQGINATSLKGGMRAWQSAGGSQV"
BASE COUNT          327 a          769 c          863 g          358 t
ORIGIN      
        1 ggatgcgggt ggcgaggtag gaccgcaccc ccgaggcgca gtggaccgcg atcgggcggc
       61 cggccgcggc cacgcggatc tcgtctatcc ggtcccgcac ctgcgtgtgc gggacgttga
      121 gtgcctcgaa caccttgccc cgcgagcact ccgccgccga ccggacatcg aggatgaggg
      181 agttctgctt ggcccactgc aggtcctggg cgtaccagac cggcatggtg ccgtccagga
      241 tgttctgcgc ggtgaaggcc aacatgttca ccgggtcctt ggccgagccg aacggcggcg
      301 cgtaggcgag gtcgaggtcg gcgaggtcgt cggcggtcat gcccgccttg atcgcggtcg
      361 cgaggacgtc gatgcgcttg tcgaccccgt cgcggcccac tgcctgcgcg cccagcaggg
      421 tgccgtccgg ggcgaacgac ccgatgaggt ggatctgctc gctgccgggg tagtagccgg
      481 cgtgtgagcc ggggtggatg tgcacggtcg tggactcgac cccgttggtc cgcagcatcc
      541 gctgcgaggc gccggtgatg gcggcgatca tcccgaagac gcgcacgatc gcggtgccga
      601 ggaccacggg tgcgggctcg ccgtcggcgg ccctgccacg catgatggcg tccgcggcga
      661 gccggccgtg gcggttggcg ggagcggcga gcggcacggg accgaggacc tcgacgtcgc
      721 ccacgcgctg gcgcacctgg acggcgtcgc cgacggccca gatgtacgca tccgaggtgc
      781 gcatcgcgcc gtcgaccgcg atggcgtcgc ggaagccgag gtcgaggccc gcgcgcttcg
      841 cgagtgcggt gtcggggacc acgccggtgg cgacgacgac aacgtcggcc ggcacaacgg
      901 tgccgtccga gagggtggcg acgacgccgg tcgccggggc gtcgtcgatg gcgacgacgg
      961 aggtccccac ccggacggtc accccgttgc gctccagctc ggcctgcagg aggggggcga
     1021 gttcgtcgtc gagtggcggc aggacgtgcg gcgcgaactc gaccagggtg acctcgaggc
     1081 cgcggtgggc gagcgcctcg acggcctcca gcccgatgaa gcccgcgccg acgacgacgg
     1141 cgcgcagcgg ggcaccctcg tccagctgct cgccggggcc gtgccccccc tcgaggccgg
     1201 cgaggtgggc catgatcgcg tccagctccg ggatggtgcg cagcgggaag acggccggat
     1261 tgtccacgcc gcggatcggc gggaccatcg ccctcgcgcc gggcgcgagg atgagcgcgt
     1321 cgtagtccag cgtgtagtcg ccccggggcc cggagaccgt gaccgtgcgg gcggcgcggt
     1381 cgatcgccgt cgcctcgctg ttgacccgca cgtcgaggtt gagctgcgac ttcagggtcg
     1441 cgggcgtgtg gagtaggagc gactgccgct cggggatctc acccgagagg tggtagggga
     1501 gaccgcagtt ggcgaaggag acatgcgggc ccctgtccag gacgatgatc tctgcggctt
     1561 cattgagacg gcgggcgcgc gcggcagcgc tcatcccgcc ggcgacgcca ccgacgatga
     1621 cgagtttcat gtggtgggtc cttagctgag ggagaggaag agcttttcga gtctcttcgg
     1681 gtccatggcc tctgacgggt cctccatgca ctggcgcagg gaactcgcga tgatcgcgaa
     1741 cccggccttg tccagcgcct tggagcaggc ggcgagttgg gtgacgatgt tctcgcagtc
     1801 cccgccctcg tccagcagcc ggatcacggc cgcgagctgg ccattggcgc ggctcaggcg
     1861 gttgcggacc gacaccaggt cctgtcctgt cagttgcatt cgtgctcctt ctacacctgg
     1921 gagccgcccg cactctgcca tgcgcgcata ccacccttca gtgaggtggc gttgatcccc
     1981 tgaacccgca gttccgacgc gccctgccgg gatcgcgacc cgctggcgca caccaccacc
     2041 accggccgcc ccttgaagcg gttcgtggag ctggcgagcc gtgcgagcgg gatgtggatc
     2101 gcctcgcgcg cgtgtcctgc cttccactcg tgattctctc gcacatccac gagaacggca
     2161 ccatccgtga cgagtgccac tgcctcctcg gccttgacgg cggcgggaag gttcgtgggg
     2221 tccagcgggg ttcgtttgag gaagtcgaag aggcccatgt ttctcctggt tcgaaggtgg
     2281 ctcaagcata gcagataccc cctggggtat acgacac
//
LOCUS       DASRKH010000322         3924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_64134, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000322 DASRKH010000000
VERSION     DASRKH010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3924
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64134"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="VFC06_05160"
     CDS             complement(<1..406)
                     /locus_tag="VFC06_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81317.1"
                     /translation="MGELRSVIAGARRIVVKIGSSSLTGSDGHLSIALVTALSDVLAA
                     ERKNGRQIVLVTSGAIAAGIGPMGLPARPRDLEMQQAAASVGQGLLVAAYTSAFASHG
                     LTVGQGLLVAAYTSAFARHGLTVGQVLLTTDDM"
     gene            complement(418..1947)
                     /gene="obgE"
                     /locus_tag="VFC06_05165"
     CDS             complement(418..1947)
                     /gene="obgE"
                     /locus_tag="VFC06_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HZL81318.1"
                     /translation="MATFVDRVTLHVTAGSGGHGVASVHREKFKPLGGPDGGNGGRGG
                     SVIVVVDAQMTTLLDYHHTPHRKAHNGKQGAGDMRHGANADDMRLAVPNGTMVVSPEG
                     EVLADLVGDGAEYVVAQGGRGGLGNAALATQKRKAPGFALLGEAGTEATVVLELKSIA
                     DVALVGYPSSGKSSLIAAMSKVRPKIADYPFTTLVPNLGVVETGGGRFTMADVPGLIP
                     GASEGKGLGHDFLRHVERCSVIAHVLDTATLESDRDPLRDLEAISNELRAYSGDAHIA
                     GVPLAERPQVIVLNKIDIPDGRELAAMVRAELDDMGLPVFEVSAVSHEGLRELGFALA
                     DMVAHERAKAPLVEKAPVVVRPRAVNETGFTVTHVRDGAEEYFQVLGAKPERWVGQTN
                     FANDEAVGYLADRLARIGVETELFAAGAVPGAQVVIGDREMGVIFDWEPTMAAGAELL
                     GRRGTDLRVEEMERNARASRAEKRAEHKERMDAKSAAREELWNERDAGHWTDPAEDEQ
                     K"
     gene            complement(2031..2282)
                     /gene="rpmA"
                     /locus_tag="VFC06_05170"
     CDS             complement(2031..2282)
                     /gene="rpmA"
                     /locus_tag="VFC06_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012398199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HZL81319.1"
                     /translation="MAHKKGASSSRNGRDSNAQYLGVKRFGGEVVNAGEILIRQRGTH
                     FHPGLNVGRGKDDTLFALEAGSVTFAQRRGRKVVDVVSS"
     gene            complement(2297..2605)
                     /gene="rplU"
                     /locus_tag="VFC06_05175"
     CDS             complement(2297..2605)
                     /gene="rplU"
                     /locus_tag="VFC06_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011692233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HZL81320.1"
                     /translation="MVYAIVKAGGRQEKVSVGDIVVVDRLRSKTGSSVELPALLLVDG
                     DKITSAAKDLAKIKVTAEVVRDERGPKIHILKFKNKTGYRKRQGHRQDLTRLKVTGIK
                     "
     gene            complement(2747..>3924)
                     /locus_tag="VFC06_05180"
     CDS             complement(2747..>3924)
                     /locus_tag="VFC06_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015771929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HZL81321.1"
                     /translation="HKGARVTSQISLAGRFLVYVPGGGVTGISRKLPDTERNRLKKLL
                     REVIPADAGVIVRTAAEGASEEELRADVDRLKRQWDSIEAEAKGGKAPQLLRGEPDMA
                     IRVVRDIFNEDFESLTIQGEDTWNSLSAYVGQVATDLAPRLHRFVGDGDVFSEHRIDE
                     QLSKGMDRKVFLPSGGSLVIDRTEAMTVVDVNTGKFVGKGGTLEETMTLNNLEAAEEI
                     VRQLRLRDIGGIIVIDFVDMLLEANRDRVLRRLIECLSRDRTKHQVAEVTSLGLVQMT
                     RKRVGQGLVEAFSETCEHCKGRGFLVHGEPVGEASEKQPEPARKRNSRGGGNKAKTED
                     SKTAAEVPALDQHDDSREAVKATLASIAAAAEKAHHAHDEHDQEAVADAPADAPTSD"
BASE COUNT          587 a         1335 c         1309 g          693 t
ORIGIN      
        1 ccatgtcgtc ggtcgtgagg agcacctggc cgaccgtgag gccgtgccgc gcgaaggccg
       61 aggtgtatgc cgcgaccagc agcccctggc ctaccgtgag gccgtgactc gcgaaggccg
      121 aggtgtaggc cgcgaccagg agcccttggc ccaccgatgc cgcggcctgc tgcatctcga
      181 ggtcgcgggg ccgcgcgggc agtcccatgg gcccgatgcc ggcagcgatc gctccggagg
      241 tgaccaggac gatctggcga ccgttcttgc gttcggctgc cagcacatcg gacaacgccg
      301 tgacgagtgc gatggagagg tggccgtcgg aacccgtcag cgacgaggag ccgatcttca
      361 ccacgatgcg cctcgcacca gcgatgacgc tgcgcagctc gcccacgatg cctccgtcta
      421 cttctgttcg tcctctgcgg gatcggtcca gtgtcccgca tcgcgttcgt tccacagttc
      481 ctcacgcgcc gcagacttcg cgtccatgcg ctccttgtgc tcggcgcgct tctccgcacg
      541 cgaggcacgg gcgttgcgtt ccatctcttc gacccgcagg tcggtgccgc ggcggcccag
      601 cagttcggct ccggccgcca tcgtcggctc ccaatcgaag atgacgccca tctcgcggtc
      661 gccgatgacc acttgggcgc ccgggacggc cccggcggca aacagttccg tctcgacgcc
      721 gatgcgcgcc aggcggtcag caaggtagcc cacggcctcg tcgttcgcga agttggtttg
      781 gcccacccag cgctccggct tggctccgag cacctggaag tattcctcgg ccccgtcgcg
      841 cacgtgcgtg acggtgaagc cggtctcgtt gacggcgcgg ggacgcacca ctacgggcgc
      901 cttctccacc agtggcgcct tggcgcgttc gtgcgcgacc atgtctgcca aggcgaagcc
      961 gagctcgcgc aggccctcgt ggctgacagc gctgacctcg aacacgggca ggcccatgtc
     1021 gtccagctcc gcgcgcacca tggccgccag ttcgcggccg tcggggatgt cgatcttgtt
     1081 gagcacgatc acctggggac gctccgcaag cgggacgccg gcgatgtggg cgtcgcccga
     1141 gtaggcgcgc agctcgttcg agatggcctc gaggtcccgc aaggggtctc ggtcggactc
     1201 gagcgtcgcg gtgtcaagca cgtgcgcaat caccgagcag cgttcgacgt ggcgcaggaa
     1261 gtcatggccg aggcccttgc cctcggatgc tccggggatc aggccgggca cgtcggccat
     1321 cgtgaagcga ccgccgccgg tctccaccac gcccaagttg ggcaccaggg tggtgaacgg
     1381 gtagtcggcg atcttgggcc gcaccttgct catggcggcg atcagggacg acttgcccga
     1441 gctcgggtag cccacaagcg cgacgtcggc gatggacttg agctcgagaa cgaccgtggc
     1501 ctcggtgccc gcctcgccca gcagcgcgaa gccgggggcc ttgcgcttct gggtggccag
     1561 cgcggcgttg ccgagtcccc cgcggccacc ttgggccacc acatactcgg cgccgtcgcc
     1621 caccaggtcg gccagcacct cgccctcggg gctcaccacc atggtgccgt tgggaacggc
     1681 caggcgcatg tcgtcggcgt tggcgccgtg acgcatgtcg cccgcgccct gcttgccgtt
     1741 gtgcgccttg cggtgcggcg tgtggtggta atcgagcagc gtggtcatct gcgcgtcgac
     1801 aacgacgatg acggagccgc cgcgtccgcc gttgccgccg tcgggcccgc cgagcggctt
     1861 gaacttctcg cggtgcaccg aggcaacgcc gtggcctccg gagccagcgg tcacgtgcag
     1921 cgtcacgcgg tcaacgaacg tggccatggc tcctccttgg tgtcgacggt atgagaaaag
     1981 gcaacagggg cgccaccaac ggtgacgccc ctgttgagaa agacttgggt ctagctggag
     2041 accacgtcga cgaccttgcg gccccgacgc tgcgcgaacg tgaccgatcc ggcctcgagc
     2101 gcgaacaggg tgtcgtcctt gccgcgaccc acgttgaggc cggggtggaa gtgggtgccg
     2161 cgctgacgga tcaagatctc gcctgcgttg acgacctcgc cgccaaagcg cttcacgccc
     2221 aggtactgag cattcgaatc gcgaccattg cgcgaggaac tcgcgccctt tttgtgtgcc
     2281 atggttcagt cctcgcttac ttgatgcctg tgaccttgag ccgcgtcaga tcctgacggt
     2341 ggccctggcg cttgcggtag ccggtcttgt tcttgaactt aagaatgtgg atcttgggtc
     2401 cacgctcgtc gcgcactacc tcagccgtga ccttgatctt ggcgaggtcc ttggcggccg
     2461 aggtgatctt gtcaccgtca accagcagca gagccggaag ctccacggac gaaccagtct
     2521 tcgaccgaag acggtccacg acgacgatgt cgcctacgga taccttctcc tgacggccgc
     2581 cggccttgac aatcgcgtac accatgatga cttcctcttg aagctcgttt actcgcggcc
     2641 cacatgggcc agggggtccc gccgcagccg ctccccgaag agggcgcagg cagggcaagg
     2701 cgcttgcgcg ccgacgggca actatacgcg tccggcggcg ctcaggtcaa tcggaggtcg
     2761 gcgcgtcagc gggtgcgtcg gcgacagcct cttggtcgtg ttcgtcgtga gcgtggtggg
     2821 ccttctccgc cgctgccgca atcgaggcca gcgtggcctt gacggcctcc cgtgagtcgt
     2881 cgtgctgatc gagggcagga acctcggcgg cggtcttgga gtcctccgtc ttggccttgt
     2941 tgcccccacc acgtgagttg cgcttgcgtg cgggctccgg ctgcttctcg ctggcttcgc
     3001 ccacgggctc gccatgtacc aggaagccgc ggcccttgca gtgctcacac gtctcggaga
     3061 acgcctccac caggccctgg cccacgcgct tgcgggtcat ttgaaccaga ccaagcgagg
     3121 tgacctcagc cacctggtgc ttggtgcggt cgcggctgag gcactcgatg aggcgacgca
     3181 acacgcggtc gcggttcgcc tcgagcagca tgtccacaaa gtcgatgacg atgatgccgc
     3241 cgatgtcgcg caggcgaagt tgccgcacga tctcttcggc cgcttcgagg ttgttgaggg
     3301 tcatcgtctc ttcgagcgtg ccgcccttgc cgacgaactt gccggtgttg acgtcgacga
     3361 ccgtcatggc ctcggtgcgg tcgatgacga gcgaaccgcc ggagggcagg aagaccttgc
     3421 ggtccatgcc cttggacagc tgctcgtcga tacggtgctc cgagaagacg tctccgtctc
     3481 ccacaaaccg gtgcaggcgc ggcgccaagt cagtcgcgac ctggcccacg taggcgctca
     3541 gcgagttcca cgtgtcctcg ccctgaatgg tgagcgactc gaagtcctcg ttaaagatgt
     3601 ctcgcaccac gcggatggcc atgtcgggct cgccacgcaa cagctgcggg gccttgccac
     3661 ccttagcctc agcctcgatc gagtcccact gacgcttgag ccggtcgacg tcggcgcgca
     3721 gttcctcctc ggaggcgcct tcggccgcgg tccggacgat cactccggcg tcggcgggga
     3781 tgacctcgcg cagcaacttc ttgagacggt tgcgttcggt gtcgggaagc ttgcggctga
     3841 tgccggtcac cccgccgcct ggcacgtaga ccaggaagcg tcccgcgagc gaaatctgcg
     3901 aggtgacgcg ggcacccttg tggc
//
LOCUS       DASRKH010000323         2152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_10732, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000323 DASRKH010000000
VERSION     DASRKH010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10732"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1346)
                     /locus_tag="VFC06_05185"
     CDS             complement(<1..1346)
                     /locus_tag="VFC06_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012802167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3516 domain-containing protein"
                     /protein_id="HZL81322.1"
                     /translation="MPTPQRPLNLAERIPADPSDPDALYEAFVGWATDQGLSLYPHQS
                     ESLLELVTGSHVILSTPTGSGKSLVAIAAHFIALAQGRRTYYTAPLKALVSEKFFSLV
                     AAFGSQNVGMMTGDSAVNPGAPIICCTAEILANITLRHGDDADVGQVVMDEFHFYADP
                     QRGWAWQVPLLELTHAQFILMSATLGDVSFFAEDITRRTGVPVAVIADAERPVPLTFS
                     YAIEPLHELLEELVRTRRSPVYVVHFTQREAVERAQALMSMTLASREQRDQIADELGA
                     FRFGPGFGKTLSRLLRHGIGVHHAGMLPKYRRVVERLTQKGLLQVVCGTDTLGVGINV
                     PIRTVVLTSLVKYDGVRMRHLSAREFHQIAGRAGRAGFDTVGEVIVLAPEHVIENRKL
                     LARAGDDPKKIKKIVRKKAPEGAVNWTDKTFERLRDAPPEPLTSSFSVTHAMVLNVL"
     gene            1417..>2152
                     /gene="ppk2"
                     /locus_tag="VFC06_05190"
     CDS             1417..>2152
                     /gene="ppk2"
                     /locus_tag="VFC06_05190"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HZL81323.1"
                     /translation="MGKKKSGAGQRGPQLSDKVYEAELFRLQSELVKVQLWARATGAR
                     IVVIFEGRDAAGKGGTIKRITQYLSPRVARIAALPTPTERERGEWYYQRYIAHLPAAG
                     EIVLFDRSWYNRAGVERVMGFCTPQEYTRFMRQTPIFEQMLIDDGILLRKYWFSVSDA
                     EQLRRFRSRLHDPARQWKLSPMDLESINRWEDFSRAKDEMLVHTDVPASPWFVVESDV
                     KKHARLNMMAHLLSTIPYSDVPRDEVM"
BASE COUNT          338 a          702 c          783 g          329 t
ORIGIN      
        1 aggacgttga ggaccatcgc gtgggtcacg ctgaagctcg acgtcaacgg ctccgggggg
       61 gcgtcgcgca gccgctcgaa cgtcttgtcc gtccagttga ccgcgccctc cggcgccttc
      121 ttgcggacga tcttcttgat cttcttcggg tcgtcgcccg cgcgcgcgag cagcttgcgg
      181 ttctcgatga cgtgctcggg ggcgagcacg atgacctcac cgacggtgtc gaaaccggcc
      241 cggcccgcgc gcccggcgat ctggtggaac tcgcgcgccg acaggtggcg catgcggacc
      301 ccgtcgtact tgaccagcga tgtgagcacg accgtgcgga tcgggacgtt gatgccgacg
      361 ccgagcgtgt cggtgccgca cacgacctgc agcagaccct tctgggtcag gcgctcgacg
      421 actcggcggt acttggggag catgccggcg tggtggacgc cgatcccgtg ccgcagcagt
      481 cgggagagcg tcttgccgaa cccggggccg aaccggaacg ctccgagctc gtcggcgatc
      541 tggtcgcgct gctcgcggct cgcgagcgtc atcgacatga gcgcctgcgc tcgctcgacg
      601 gcctcgcgct gggtgaagtg cacgacgtag accggggagc gccgggtccg gacgagctcc
      661 tcgagcagct cgtggagcgg ctcgatcgcg taggagaacg tgagcgggac gggccgctcg
      721 gcgtccgcga tgacggccac gggcacaccg gtgcggcgcg tgatgtcctc ggcgaagaag
      781 ctgacgtcgc cgagcgtcgc ggacatgagg atgaactgcg cgtgcgtgag ctcgagcagc
      841 gggacctgcc acgcccaccc gcgctgcggg tcggcgtaga agtggaactc gtccatgacg
      901 acctggccga cgtccgcgtc gtcgccgtgc cgcagcgtga tgttcgcgag gatctccgcg
      961 gtgcagcaga tgatgggcgc gccggggttg accgccgagt cgcccgtcat catgccgacg
     1021 ttctgcgagc cgaaggccgc gacgagcgag aagaacttct cgctcacgag cgccttgagc
     1081 ggggccgtgt agtacgtgcg ccggccctgg gcgagcgcga tgaagtgcgc cgcgatcgcg
     1141 acgagcgact tccccgaccc ggtgggggtc gagaggatga cgtgcgagcc cgtgacgagc
     1201 tcgagcagcg actccgactg gtgcgggtag agcgagaggc cctggtcggt cgcccagccc
     1261 acgaacgctt cgtagagagc gtcgggatcg gaggggtcgg cagggatccg ctcggcgagg
     1321 ttcaacgggc gttggggagt gggcactggt ccatcctccc agcctcgagc gccgttgggg
     1381 gcgcgacgtg ggctccgcga gggctaacgt cccgtcatgg gcaagaagaa gtcgggcgcg
     1441 ggtcagcgcg gtccacagct ctccgacaag gtctacgagg ccgagctgtt caggctgcag
     1501 tcagagctcg tcaaggtcca gctgtgggcc agggcgacgg gtgcacggat cgtcgtgatc
     1561 ttcgagggcc gcgacgccgc cggcaagggc ggcacgatca agcgcatcac gcagtacctg
     1621 agcccgcgcg tcgcgcgcat cgccgcgctc ccgacgccga ccgagcgcga gcgcggcgag
     1681 tggtactacc agcgttacat cgcgcacctg cctgccgcag gggagatcgt cctgttcgac
     1741 cggtcctggt acaaccgggc cggggtcgag cgcgtcatgg ggttctgcac gccgcaggag
     1801 tacacgcgct tcatgcgcca gacgcccatc ttcgagcaga tgctcatcga cgacggcatc
     1861 ctgctgcgca agtactggtt ctccgtctcg gacgccgagc agctgcggcg cttccggtca
     1921 cgcctgcacg acccggcgcg gcagtggaag ctcagcccga tggacctgga gtcgatcaac
     1981 cgctgggagg acttctcccg cgcgaaggac gagatgctgg tccacaccga tgtgcccgcg
     2041 agcccgtggt tcgtcgtgga gtccgacgtc aagaagcacg cgcggctcaa catgatggcg
     2101 cacctgctct cgacgatccc gtacagcgac gtgccgcgcg acgaggtcat gc
//
LOCUS       DASRKH010000324         2388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_62269, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000324 DASRKH010000000
VERSION     DASRKH010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62269"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..837)
                     /locus_tag="VFC06_05195"
                     /pseudo
     CDS             complement(1..837)
                     /locus_tag="VFC06_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882246.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
     gene            complement(971..1642)
                     /locus_tag="VFC06_05200"
     CDS             complement(971..1642)
                     /locus_tag="VFC06_05200"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HZL81324.1"
                     /translation="MTNPVFLADELPEGAGSFALAGAEGHHAHVMRLGPGAGIDVADG
                     RGNRLRGVVAEVTGDGVRIDVASVTHEGDDVELVLVQALAKGGRDEEAIEMATEVGAD
                     AIVPWQADRSIAQWPAAKATCAAARWESVLRAATKQSRRSRVPALLELMDSRRLEAFV
                     RRTVADGGVVLVLHEAADRTLDAVGLPRAGRVVVVVGPEGASPRRNWRRCPPPKRCRS
                     WPGRT"
     gene            complement(1639..>2388)
                     /locus_tag="VFC06_05205"
     CDS             complement(1639..>2388)
                     /locus_tag="VFC06_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HZL81325.1"
                     /translation="VFGATREITVNTAVVCPTCSGTCCRPGTSPRTCEVCGGRGSVQR
                     VARSFLGQIMTTSPCAMCQGHGTIIADPCTECSGDGRVRTRRTIPVDVPAGVENGARL
                     RMPGAGEVGPGAGPAGDLHIEFRVRPHDRFTRRGDDLLCSVTVPMTAAALGTVLTIDT
                     FDGPREVTIKPGTQPGHTELLPGLGVTRLYHHVRGDLKVFVDVEMPARLDDRQRELLA
                     ELARVRGEERVNPAVTGGSGMFARLKEKIGL"
BASE COUNT          316 a          847 c          882 g          343 t
ORIGIN      
        1 caggccggac cgctgaccgc tcggcaggtc gacctgcgtg acatcaccgg tgacgaccat
       61 cgtcgacccg aagccgagcc gggtgaggaa catcttcatc tgctcggcgg aggtgttctg
      121 ggcctcgtcg aggatgatga acgcgtcgtt gatggtgcgg ccccgcatgt aggcgagcgg
      181 cgccacctcg atggtgccgg cggccatgag cttcgggatc gagtcggggt cgatcatgtc
      241 gtgcagcgcg tcgtagagcg ggcgcaggta cgggtcgatc ttctccgaga gggtgccggg
      301 gaggaagccg agccgctccc ccgcctccac ggctgggcgg gtgaggacga tgcgcgtgac
      361 ctgcttggac tgcagggcat tcacggcctt cgccatcgcc aggtacgtct taccggtgcc
      421 ggcgggcccg atgccgaagg tgatcgtgtt ggagtcgatc gcgtccacgt aggccttctg
      481 tccgatggtc ttcggccgga tggtccgccc gcgcgaggag atgatgttgg tggtcatcac
      541 ctccgacggg ctcgcggcat gcaccgtgag catcccgatg gcgcgctgca cgccatccac
      601 gctcaacggc gccccggacc gggccaccag gacgagttat tcgaggaggc gctgcgccgt
      661 cgtcacgtcc cccaccgggc cggtgagcgt caccacgttg ccgcgggcgt gcacctccac
      721 cctgctgaac ccggcctcga cggtgcggag cacctcgtcg cgcgtgccga acagcgtcac
      781 ggggtggacg tcgtcgggga tggtgacgcg gtaggtgacg gattcgggtg atgtcatgcg
      841 ggtccctcca cgccccgtgt gggccttcgg ctccccagtg tatcggcggc gctccgcggt
      901 cccacgcccc agcgcccgat ggcgcgcgcc acgtgcgaga tcgcgacggg tcccgccgtg
      961 ccggagcgca tcacgtgcgg cccggccagg accggcaccg cttcggcggc ggacatcgcc
     1021 gccagttcct gcggggtgat gccccctccg gaccgacgac caccaccacg cgccccgccc
     1081 gcggcagccc gacggcgtcc agggtgcggt ccgcggcctc gtgcagcacg agcaccacgc
     1141 ccccgtccgc cacggtgcgg cggacgaacg cctccagccg gcgggaatcc atgagctcga
     1201 ggagggccgg gacccgcgag cggcgcgact gcttggtggc ggcgcgcagc accgactccc
     1261 acctggccgc ggcgcacgtg gccttcgcgg ccggccactg ggcaatggag cggtcggcct
     1321 gccaggggac gatcgcgtcc gccccgacct ccgtggccat ctcgatcgct tcctcgtcgc
     1381 ggccaccctt ggcgagggcc tggacgagga cgagttcgac gtcgtcgccc tcgtgcgtca
     1441 ccgaggcgac gtcaattcgc acgccgtcgc ccgtcacctc cgccacgacg ccgcgcaggc
     1501 ggttgccacg gccgtcggcg acgtcaatcc ccgcgccggg gcccaggcgc atcacgtgcg
     1561 cgtggtgacc ctcggcgccg gccagggcga acgacccggc gccctccggc agctcatccg
     1621 ccaggaagac ggggttggtc acaggccgat cttctctttc agccgcgcga acatgccgct
     1681 gcctccggtg acggcgggat tgacgcgctc ctcgccccgg accctcgcga gctcggcgag
     1741 cagttcccgc tggcggtcgt ccaggcgggc gggcatctcc acgtccacga acaccttcag
     1801 gtcgccgcgg acgtggtggt acaggcgggt gacgccgagt cccgggagga gctccgtgtg
     1861 gcccggctgc gtgcccggct tgatggtgac ctcgcgtggg ccgtcgaacg tgtcgatggt
     1921 cagcacggtg ccgagggccg ccgccgtcat gggcacggtc acagagcaga ggaggtcgtc
     1981 gccgcggcgg gtgaagcggt cgtggggccg gacgcggaac tcgatgtgga ggtctccggc
     2041 gggcccggcg cccgggccga cctcgcccgc tccgggcatc cgcaggcggg caccgttctc
     2101 gacgccggcg ggcacgtcca cggggatggt gcggcgggtg cggacgcggc cgtccccgga
     2161 gcactcggtg caggggtccg cgatgatggt gccgtggccc tggcacatgg cacacgggct
     2221 ggtggtcatg atctggccga ggaaggaccg cgccacacgc tgcaccgagc cacgtccgcc
     2281 gcacacctcg caggtgcggg ggctggtgcc ggggcggcag caggtgccgg agcacgtggg
     2341 gcacaccacg gcggtgttca ccgtgatctc gcgggtcgcg ccgaagac
//
LOCUS       DASRKH010000325         2481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_37176, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000325 DASRKH010000000
VERSION     DASRKH010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37176"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="VFC06_05210"
     CDS             <1..937
                     /locus_tag="VFC06_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HZL81326.1"
                     /translation="PVGTTRERWKPMLEQVSGLTEGKDFHLVFSPERVLTGRVFADLR
                     KYPKLVGGLSAEGAARATAFYEAVLDFDDRPDLVRPNGVWDLGSAEAAELAKLAETTY
                     RDVNIGLANQFARFAATHGIDVYQVIEASNSQPYSHIHQPGIAVGGHCIPVYPRLYLW
                     NDPEATVVAAARAANAGMPDYTIGLLEGAYGDLTDARVVVLGASYRGGVKETAFSGVF
                     AAVDALKARGAAPVVHDPMYTDDELERLGFDPFHLGEDIDAAVVQADHAEYRELGPAD
                     LPGLRVFIDGRRVSTAERWPDVQYRLIGKALAPNS"
     gene            complement(927..2453)
                     /locus_tag="VFC06_05215"
     CDS             complement(927..2453)
                     /locus_tag="VFC06_05215"
                     /inference="COORDINATES: protein motif:HMM:NF021436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HZL81327.1"
                     /translation="MATVWRRIGLRRLIGRLRWIHAAPLLAVLTLTAWAFASPVGAAP
                     DDDYHLVSTWCALGGSEACTPVADPNLRRVSTAFLSSTCYALVSERSAACQEEAGLEL
                     DGPTSVTDRGNFHHKYPGVYYGTMRLFAGDDLVRSAVVMRLVNLTLFVALATALAALM
                     PASRRQTLLWGWLVSLMPLGVFLIPSNNPSGWAITGVGTAFLALLGWFESTGRRRWAL
                     GGLYAVGIVMAAGARSDAAIYAAGATLVASLLTWSRQRDWVRPSLLPLGGLIIIGVLF
                     FTTNQAGVGADGIAGGELGTFSGTSEQLFVHNVFELPYLWTGVWGTWALGWFDTQLPA
                     VVPWAAMGAFVTVAFAGLGLLTKRKTVAVAGVLAVLVVLPVYLLTAGGDVVGSQFQPR
                     YLLPLIVLLSLVLVTAPSGSRTVRFTGVQTFAILGALAIANLVALQVNIRRYVTGSDQ
                     QGFNLGAGAEWWWHDLPVGPMAVWLLGSLSFAGLLAVLWPELRRKVVAPSGADRELRS
                     "
BASE COUNT          495 a          877 c          757 g          352 t
ORIGIN      
        1 ccccgtgggc accacgcgcg aacgctggaa gccgatgctc gagcaggtct ctggcctcac
       61 cgaaggcaag gacttccacc tggtgttctc gcccgagcga gtgctgaccg gccgcgtgtt
      121 tgcggacctg cggaagtacc ccaagctcgt gggcggcctg tcggccgagg gcgccgcgcg
      181 cgccaccgct ttctacgagg ccgttctcga cttcgacgat cgccccgacc tggttcgccc
      241 caacggtgtg tgggaccttg gctccgcgga ggccgcagag ctcgccaagc tcgccgagac
      301 cacgtaccga gacgtcaaca tcggccttgc caaccagttc gcgcgctttg cggcgacgca
      361 tgggatcgac gtctaccagg tgatcgaggc gtcgaactcg cagccctaca gccacatcca
      421 ccagccaggc atcgccgtcg gcggtcactg catccccgtc tacccgcgcc tgtacctgtg
      481 gaatgacccc gaggcaacag tggtggccgc ggcacgcgcc gcgaatgccg ggatgcccga
      541 ctacacgatc ggcctgctcg agggcgccta cggcgacctg acggatgccc gcgttgtggt
      601 gctcggtgcc tcctaccgtg gcggcgtgaa ggagacggcc ttctccggag tcttcgcggc
      661 cgtcgacgcg ctcaaggcgc gcggcgccgc ccccgttgtt cacgatccga tgtacaccga
      721 cgacgagctc gaacggctcg gcttcgaccc gttccacctc ggcgaagaca tcgacgccgc
      781 agtggttcag gcagaccacg ccgagtaccg ggagctcggc cccgctgacc tgcccggcct
      841 cagggtcttc atcgacggac gccgcgtatc gaccgccgag cgctggcccg acgtccagta
      901 ccgcctgatt ggcaaggcgc tcgcacctaa ctcctgagct cccggtcggc gcccgaggga
      961 gcgacgactt tacgcctgag ttcgggccac agcaccgcga gaagcccggc gaaggacaac
     1021 gagccaagga gccacacggc catggggccc acgggcagat cgtgccacca ccactctgcc
     1081 ccagcgccaa ggttgaatcc ctgctggtct gagcccgtga cgtagcggcg gatgttgacc
     1141 tgcagcgcca ccaggttggc gatcgcgagt gccccgagaa tcgcgaaagt ttgaacaccg
     1201 gtgaagcgca ccgtccgcga gccagagggt gccgtgacaa gcacgagcga aagcaacacg
     1261 atgagcggca ggagatagcg cggctgaaat tggcttccca ccacgtcccc ccccgcggtg
     1321 agaaggtaca caggcaagac cacgagcacc gcaagtacac ccgcgacagc tacggtcttg
     1381 cgcttggtca gcaacccaag cccggcgaaa gcgacggtga cgaacgcacc catcgcagcc
     1441 catgggacga ccgcaggcaa ctgagtgtcg aaccacccca gcgcccacgt gccccatacg
     1501 ccagtccaca ggtacggaag ctcgaagacg ttatgcacga agagctgttc cgacgtacca
     1561 gaaaaagtgc caagctcccc accggcgatg ccatctgcgc ccacaccggc ttggtttgta
     1621 gtgaagaaga gcacgccaat gatgatgagg ccgccaaggg gaaggagcga cggccgcacc
     1681 cagtctcgtt gacgcgacca cgtcagcagt gacgccacca aggttgcgcc cgccgcgtag
     1741 atcgcggcgt cgcttcgcgc tcccgcagcc atgacgatcc cgaccgcata tagcccgccc
     1801 agcgcccacc gccgtctgcc ggtgctttcg aaccacccca acagcgccaa gaaagccgtg
     1861 ccaacaccgg taatggccca accgctcgga ttgttcgacg ggatgaggaa gactcccagt
     1921 ggcatcaagg acacgagcca accccacaac aacgtttggc ggcgcgaggc cggcatcagc
     1981 gccgccagtg ccgtcgccag tgccacaaac agcgtgaggt tcaccaggcg catcaccacc
     2041 gcactgcgca ccaggtcatc gccagcgaac aagcgcatcg tcccgtaata gacacccggg
     2101 tacttgtggt ggaagtttcc gcggtcggtt acggacgtcg gcccatcgag ctcaagccca
     2161 gcctcttcct ggcacgccgc actacgctcc gacaccaggg catagcaggt tgaagaaagg
     2221 aaggccgtcg acacccgtct gaggttcggg tccgcgacag gcgtacacgc ttcgctcccg
     2281 ccaagagcgc accacgtgct gacgaggtgg tagtcgtcgt caggcgccgc acccacaggc
     2341 gaagcaaagg cccaggcggt caacgtcagt acggccaaca gcggcgcagc gtgaatccac
     2401 cggagccggc cgatcagccg acgcagaccg attcttctcc agactgtcgc cacaacgtct
     2461 ccttaggtac ttgaccgggg t
//
LOCUS       DASRKH010000326         2262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_1701, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000326 DASRKH010000000
VERSION     DASRKH010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1701"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..1125)
                     /gene="rfaD"
                     /locus_tag="VFC06_05220"
     CDS             complement(148..1125)
                     /gene="rfaD"
                     /locus_tag="VFC06_05220"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR02197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HZL81328.1"
                     /translation="MSDTIIVTGAAGFIGRNIVAALNDKGHRDILLVDELGTSDKWRN
                     LRGLDFDEFISPSALMEYLDSPKGVDVRAIIHMGACSATTERDADYLVANNYRYTLDL
                     ITWSLAHNARMIYASSAATYGDGSLGYSDADDVTPTLRPLNMYGYSKQMVDEWALRNG
                     LLESIVGLKFFNVYGPYEEHKGDMRSLVSKAYRQVRDTGQITLFRSHRPEYAHGEQMR
                     DFIYVDDAVDVVLYFLDHPEHNGLFNCGTSHAATWLELTNALFGALGRQPQVEFVDMP
                     EAIRDRYQYFTEADGTKLREAGYPGPFRGVDAGVREYVETFLSRELADD"
     gene            complement(1122..1619)
                     /gene="rfaE2"
                     /locus_tag="VFC06_05225"
     CDS             complement(1122..1619)
                     /gene="rfaE2"
                     /locus_tag="VFC06_05225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HZL81329.1"
                     /translation="MMTSYGREAITPQQMREIRDEMAAEGRTLVFTNGGFDILHPGHV
                     RYLQFARDQGGALVVGLNSDASVRRGKGPKRPINSEEDRAEVLLALGSVDHVVVFDED
                     EPRDLIAAIRPQVLVKGSDWAHYVSGRETVEADGGKVVLAEMVEGRSTTSTIERVLAA
                     YGGAS"
     gene            complement(1652..>2262)
                     /locus_tag="VFC06_05230"
     CDS             complement(1652..>2262)
                     /locus_tag="VFC06_05230"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZL81330.1"
                     /translation="QTLLQMRGLIALVREWGFVPGAQVLDEHGYKAIYNDLLIDHVNQ
                     RVAKLRAGQLAREDFHMKNALPLLQALRDAGLKLHLASGTDEADVIAEANELGFGEFF
                     GDRIYGSTGVVTHDAKRMVIERILTENELDGSSLLTFGDGPVEMRETRRRGGIAVGVC
                     SDEVRRYGFNAGKRRRLIRGGAHLLVGDYSDLDTLLTTLHLA"
BASE COUNT          397 a          734 c          710 g          421 t
ORIGIN      
        1 gtgtagaccg tgccgccggc gtcgaaaatg tcacgcatca gccgcgccac cgccacgacg
       61 tccggcaact gctgcgtcat tgcggcgccc gcttgcacgt agtcggcgag ttgtgcggtt
      121 gcccatgcgg gcacgccaag cgacgggtca gtcatcggcc agctcccgtg acaggaacgt
      181 ctcgacgtac tcgcgcacgc ctgcgtccac gccgcggaac gggccggggt accccgcctc
      241 ccgcagcttg gtcccgtcgg cctccgtgaa gtactggtac cggtcgcgaa ttgcctcggg
      301 catgtcgacg aattcgacct gcggctgtcg gcccagcgca ccgaacagcg cgttcgtcaa
      361 ttccagccac gtcgcggcgt gagaggtgcc acagttgaac agtccgttgt gctcagggtg
      421 gtcgaggaag tacagcacaa cgtccacggc gtcgtcgacg tagatgaagt cacgcatctg
      481 ctccccgtgg gcgtactcgg gacgatgcga gcggaacaat gtgatctgtc cagtatcgcg
      541 cacctgacgg taggccttgg agaccagcga gcgcatgtcg cccttgtgct cctcgtaagg
      601 gccgtacacg ttgaagaact tcagcccgac aatggattcg agcagcccgt ttcgcagcgc
      661 ccactcgtcg accatctgct tggagtagcc gtacatgttg agaggccgaa gggtgggggt
      721 gacgtcatcg gcatcgctat agcccaggga cccatcccca tacgttgccg cgctcgacgc
      781 gtagatcatg cgcgcgttgt gggcgagcga ccaggtgatc aggtcaagcg tgtatcgata
      841 gttgttggcg acgaggtagt cggcgtcacg ctcggtcgtc gcgctgcacg cgcccatgtg
      901 gataatcgcg cgtacgtcta ctcctttcgg cgaatcgaga tactccatca atgccgaggg
      961 cgaaatgaac tcgtcgaaat cgaggccacg caggttgcgc cacttatcgg acgtgccgag
     1021 ctcgtcaacc aggaggatgt cgcggtgtcc tttgtcattc agcgcggcga cgatgttgcg
     1081 tccgatgaaa ccggcggcgc ctgtgacgat gatcgtgtcg ctcatgaggc acccccatag
     1141 gccgcaagca ctcgttcaat cgtgcttgtc gtcgagcggc cctcaaccat ctcggcgagc
     1201 accaccttgc caccgtcggc ctcaaccgtc tcgcgaccgc tcacgtagtg agcccagtcg
     1261 ctgcccttga ccagcacctg agggcggatc gcggcgataa ggtcccgggg ctcgtcttcg
     1321 tcgaacacga ccacgtggtc gaccgagccc agcgcgagca gcacctcggc gcggtcttcc
     1381 tcggagttga tcgggcgctt gggtcccttg ccgcggcgaa ccgaggcgtc ggagttcaac
     1441 ccgaccacca aggcaccgcc ctggtcacgg gcgaactgca ggtagcgcac gtgtcctggg
     1501 tgcaggatgt cgaagccccc gttggtaaac acgagggtgc ggccctcggc cgccatctca
     1561 tcgcggatct cacgcatctg ctgcggcgtg atcgcttcgc gtccgtaact tgtcatcatt
     1621 cctcttctcg tcagataggt cggggggctt ctcaggccag gtgcagggtg gtgagcaacg
     1681 tgtcaaggtc gctgtagtcg ccgacgagca ggtgcgcccc accgcggata aggcggcgac
     1741 gcttaccggc attgaagccg tagcgtcgca cctcgtcgct acacacaccc actgcgatgc
     1801 cgccgcggcg gcgtgtctcg cgcatctcaa ccgggccgtc accgaaggtg agcaaactcg
     1861 acccgtcgag ctcgttctcc gtcaggatcc gttcgatcac catgcgcttg gcatcgtgcg
     1921 tcactacccc ggtcgagccg tagatgcggt caccgaagaa ctcaccgaag ccgagctcgt
     1981 tggcttctgc gatgacatcg gcctcgtcag tgccgctcgc gagatgcaac ttgagaccgg
     2041 cgtcgcggag cgcctgcagc aacggcaggg cgttcttcat atggaagtcc tcgcgggcga
     2101 gctgacctgc tcgcaacttg gctacgcgct gattgacgtg gtcgatcagg agatcgttgt
     2161 agatcgcttt gtacccgtgc tcatccagga cctgcgcgcc cggcacgaac ccccactcgc
     2221 gcacgagggc gatgagcccg cgcatctgca gcagggtctg ga
//
LOCUS       DASRKH010000327         2133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_5197, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000327 DASRKH010000000
VERSION     DASRKH010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5197"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /locus_tag="VFC06_05235"
     CDS             complement(<1..1097)
                     /locus_tag="VFC06_05235"
                     /inference="COORDINATES: protein motif:HMM:NF013216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HZL81331.1"
                     /translation="MSDPQRRAALDCVLAVEVDGAYANLAMPGILRQARLGGREAAFA
                     TELAYGALRMSGLYDAVIAKAANRRPDSLDVTVRAVLWLGAHQALSMSTPVHATVSET
                     VALAKDAGAARASGFVNAVMRRIVERDREAWLALVAAGTGRAAVSTRHSHPQWIVAEL
                     ERSLAARGRAGEVELLLAAHNTPAAVTLAARPGLADRDEVVRATGGEPTILSPYGITL
                     GGGAPRGLADVEAGLVGVQDEGSQVVAAALTTEAARPSSAGERWLDACAGPGGKTALL
                     GAIAAQRDAHLDAVELHAHRARLVEQNVRALPAGVVTVHVGDSTTWEGGPYDRILLDA
                     PCTGIGALRRRPESRWRRSEDDLADLSGLQA"
     gene            complement(1094..2026)
                     /gene="fmt"
                     /locus_tag="VFC06_05240"
     CDS             complement(1094..2026)
                     /gene="fmt"
                     /locus_tag="VFC06_05240"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HZL81332.1"
                     /translation="MRLIFAGTPEVALPSLEALVDAGHEIVLVITKPDAKGKRGTATY
                     PSPVKTRALELGLEVASPVKASEPVFVERVRSLRADAAGVVAYGQILRPALLEATRFG
                     WVNLHFSVLPAWRGAAPVQRAIQAGDDVTGASTFLIEEGLDTGPVIGRATERIRPTDT
                     AGDLLTRLASVGAPLLKDSLEALGAGIATPLAQAEDGVSHAAKLTRDDALIRWDLPAH
                     FVDRAIRACTPAPGAWTTLPDGSTAKVGPVSLTDAPSTTPGLIRADAEGVTVGTGGVP
                     VRLTWIQPAGKKAMDAGDWWRGARWGETAQLGEA"
BASE COUNT          321 a          775 c          722 g          315 t
ORIGIN      
        1 ctcgcctgca ggccactgag gtctgcaagg tcgtcctctg agcgcctcca cctcgactcg
       61 ggccgccgcc gaagcgcgcc aatcccggta catggggcat caagaaggat gcggtcatac
      121 ggaccgccct cccacgtcgt cgagtcgcct acgtgaacgg tgacgacgcc agccggaagg
      181 gcgcgcacgt tctgctcgac aagccgcgcc cggtgggcgt gaagctcgac cgcatccaag
      241 tgcgcgtccc gctgggccgc gatcgcccca agaagtgccg tcttgccgcc tgggccggcg
      301 catgcgtcga gccatcgttc ccccgccgat gacggccttg ccgcctcggt ggtgagggcg
      361 gctgccacca cctgacttcc ctcgtcctga acacccacga gtccggcctc gacgtccgcg
      421 agacctcgcg gcgcccctcc gcccagcgtg atcccgtaag gagagaggat ggtgggttcg
      481 ccgcccgtgg cacgcacgac ctcgtccctg tcggcgagcc ctgggcgcgc ggcgagagtg
      541 acggccgccg gcgtgttgtg ggcggccagg agcaattcga cctcgcccgc gcggccgcgt
      601 gcagccaggg accgttccag ttccgcgaca atccactgcg ggtgggaatg ccgagtggag
      661 accgccgccc ttccggtccc ggccgcgacg agagccagcc acgcttcgcg gtcccgctcg
      721 acgatgcgac gcatcaccgc attgacgaat ccactcgccc gcgcagcgcc ggcgtccttc
      781 gccaatgcga ccgtctccga gaccgtggcg tggaccgggg tgctcatcga cagggcctgg
      841 tgggcgccca gccacagcac ggcgcgcacc gtgacgtcca gggaatcggg gcgcctgttg
      901 gctgccttgg cgatcacggc gtcgtacagg ccgctcatgc gcagcgctcc gtaggccaac
      961 tcggtcgcga acgccgcctc gcggccgccg agtcgtgcct gccgcaggat tccgggcatg
     1021 gccaggttcg cgtatgcgcc gtcgacttct acggcaagaa cgcagtcgag cgccgcgcgg
     1081 cgctgcggat cactcatgct tcacccaact gggcggtttc gccccatcgg gctcctcgcc
     1141 accaatcgcc ggcgtccatc gccttcttgc cagcgggctg aatccaggtc aagcgaaccg
     1201 gcacaccacc ggtccccacg gtgactccct cggcatccgc acggataagc cccggcgtcg
     1261 tggagggcgc gtccgtgagg gacacggggc ccacctttgc cgtcgagccg tcgggaagcg
     1321 tggtccacgc gcccggcgcg ggtgtgcagg ccctgatggc ccgatccacg aagtgcgcgg
     1381 gcaggtccca gcggatcagt gcgtcgtccc gcgtgagctt tgccgcgtgg ctcacgccgt
     1441 cttcggcctg cgcgagcgga gtggcgatgc ccgctcccaa cgcttccagg gagtccttca
     1501 ggagcggtgc gcccactgag gccaggcgag tcagcaggtc ccctgccgta tccgtcgggc
     1561 ggatccgctc ggtggccctg ccgatcaccg ggccggtgtc gaggccctcc tcgatcagga
     1621 acgtcgaggc gcccgtcacg tcgtctcccg cctgaatggc acgttgcacg ggagcggccc
     1681 ccctccatgc cggaagcacc gagaagtgca ggttgaccca gccaaaacgg gtggcctcca
     1741 acaaggctgg acgcaggatc tgcccgtagg cgacaacccc tgcggcgtcg gcccgaaggg
     1801 accgtacccg ctcgacgaaa acgggctcgg aagccttgac aggggacgca acctcgagac
     1861 ccagttcgag agcccgggtc ttgacgggac tcgggtaggt cgcggtgccg cgctttccct
     1921 tggcgtccgg cttggtgatc accagcacga tctcgtggcc cgcgtcgacg agcgcttcga
     1981 gggatggcag tgccacctcg ggggttccgg caaagatcag acgcatgggc tccatcgtag
     2041 gcgccgacta ggtgcctgga accgcgtcga ctcgcaagaa caggggtggc gccgacgccg
     2101 ccgaccgcgc gacggccacg gcccgggcgg ctt
//
LOCUS       DASRKH010000328         2372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_30714, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000328 DASRKH010000000
VERSION     DASRKH010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30714"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1351
                     /locus_tag="VFC06_05245"
     CDS             <1..1351
                     /locus_tag="VFC06_05245"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZL81333.1"
                     /translation="WTGAGMLVAAFESTVVFVVMALYSWQLALVVLACYIPLLVVTMR
                     IQPVIRRAFSDIRARMADLLGRTSEQLVGVATIRSYGVQERMRSRLREEIDGIARRER
                     RAAWLASTSFSMTAFGQSLATGAALVVGTALGVRGDLSVGTVAAFALLVYMFSGPLMW
                     IIEMLAEMQRAMVGWQRVIELVDAPTTVAEPESPIPLPDGEVDLTVESVRLTYDDDEE
                     VLKGISLTVPAGKRLAVVGQTGSGKTSLARLLSRFFDPTSGHIRLGGVDVRDVSFAEL
                     RSRLAVVPQEGFLFEGTVLDNLLYARPDTDPVGLRTAAESAFNELGLSSWLETTVHGL
                     DTEVGARGELLSAGERQLVSIARAYLRDPDVLILDEATSAVDPATEVRISRALERLMK
                     GRTSVVIAHRLSTAERADLVAVMDSGELVEFGPHTALVGADGAYARLHRAWVAQTRD"
     gene            1388..1747
                     /gene="hisI"
                     /locus_tag="VFC06_05250"
     CDS             1388..1747
                     /gene="hisI"
                     /locus_tag="VFC06_05250"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HZL81334.1"
                     /translation="MPLDPEIAQRLKRNEAGLVCAVVQDAATRRVLMVAWMNDDALEE
                     TLRTRRGVYFSRSRNEIWRKGETSGHVQHVISVELDCDGDAVLLMVEQTGGACHTGKN
                     SCFDEGGPLDLAVGSAS"
     gene            1744..>2372
                     /locus_tag="VFC06_05255"
     CDS             1744..>2372
                     /locus_tag="VFC06_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HZL81335.1"
                     /translation="MTPPPVAAPMLDWGDTWPSRDEFVALGATHRVVPVARRLLGDGW
                     TPVAVYAALAASRPGTFILESAQPDGSRSRYSFVGVRSQAALTIVGDSARWTGDVPAG
                     LDDGAPLDTIRQALEVLASEQILGLPPLTGGLVGALGWDIVRQWEPTLPADAVRELDL
                     PDAYLLLATDLAVLDHVDGSVWLIANAVNTDAQPTGIDEAHASAVARVDA"
BASE COUNT          355 a          770 c          830 g          417 t
ORIGIN      
        1 gtggacgggc gctggcatgc tggtcgccgc cttcgagtcc acagtggtgt tcgtggtgat
       61 ggccctctat tcgtggcaac tcgcgctggt ggtactcgcg tgctacatcc ccttgctggt
      121 ggtgacgatg aggatccagc ccgtcatccg gcgagcgttc tccgacatca gggctcgcat
      181 ggccgacctg ctgggccgca cctccgagca actcgtgggt gtcgcaacga ttcgctcgta
      241 cggtgtccag gagcggatgc gatcgcgact gcgtgaggag atcgacggca tcgcgagacg
      301 ggaacgccgc gcagcctggc tggcctctac gagcttctcc atgacggcct tcggtcagtc
      361 cctcgcgacg ggcgcagccc tcgtcgtggg taccgcgctg ggcgtcaggg gcgacttgtc
      421 ggtgggaacg gtcgccgcct tcgcactgct cgtgtacatg ttctccgggc cgctcatgtg
      481 gatcatcgag atgctcgccg agatgcagcg ggcgatggtc ggttggcaac gcgtcatcga
      541 gttggtcgac gcgcccacca cggtggccga gcccgagtcg ccgattccgc ttcccgacgg
      601 cgaggtggac ctcaccgtgg aatcggtgcg gctgacgtat gacgatgatg aggaggtgct
      661 caagggcatt tcgctgaccg ttccggccgg caaaaggctc gcggtggtgg ggcagacggg
      721 gtcaggaaag acctcgctcg cgcgcctgct atcgcgattc ttcgacccga cgtcgggaca
      781 catcaggctg ggcggtgtcg acgttcgcga tgtcagtttt gcggagttgc ggtcgcgact
      841 ggccgtggtg cctcaagagg gcttcctgtt tgaagggacg gttctcgaca acctgctcta
      901 cgcgcgcccc gatacagacc cggttgggct gcgcaccgcc gcggagtctg cattcaacga
      961 gctcgggctc tcctcatggc tggagacgac ggtccacgga ctcgacaccg aggtgggcgc
     1021 gcgtggcgag ctgctgagcg ccggagagcg gcagttggtc agcatcgcga gggcatacct
     1081 gcgcgacccc gacgtgctca tcctcgacga ggcgacgtcg gcagtggatc ccgccaccga
     1141 ggtgcggata tcccgcgcgc tggagcggct gatgaagggc cgaaccagcg tcgtgattgc
     1201 ccaccgcctg tcgacggccg agcgcgcaga cctcgtcgcc gtcatggatt ccggcgagct
     1261 cgtcgaattc ggaccccaca ccgcattggt cggagcagat ggggcgtatg cacgccttca
     1321 ccgcgcatgg gtggctcaga cgcgtgactg accggcgcgg cgcgcccggc gtggaaggat
     1381 aggcgccatg cctttggacc ccgagatcgc gcagcgactc aagcgcaacg aagccggcct
     1441 ggtatgtgcc gtcgtgcaag acgccgccac ccggcgggtg ctgatggtcg cgtggatgaa
     1501 cgacgacgct cttgaggaaa cgctgcgcac gcgccgcggc gtgtacttca gccgtagccg
     1561 caacgagatc tggcgcaagg gcgagacgtc cggtcacgtc cagcacgtca tcagtgtgga
     1621 actggattgt gacggcgatg ccgtactgct gatggtcgag cagaccgggg gagcgtgcca
     1681 cacgggcaag aactcgtgct tcgacgaggg cggacccctc gatctcgccg tggggagcgc
     1741 gtcgtgacgc cgccgcccgt cgcggccccg atgctcgatt ggggcgacac atggccctcg
     1801 cgcgatgagt tcgtcgcact cggcgccaca caccgagtgg tgccggtcgc gcgccggcta
     1861 ctcggcgacg gctggacccc ggtggccgtg tacgcggccc tcgccgcgtc ccgccccggc
     1921 acgttcattc tcgagtcagc ccaacccgac ggctcgcgct cgcgctattc gtttgtcggg
     1981 gtgcgctcgc aggccgcgct cacgatcgtg ggcgattccg cgcggtggac gggtgacgtc
     2041 cctgcgggtc tcgatgacgg cgcccccctc gacacgatca ggcaggctct cgaggtcctt
     2101 gcctccgagc agattctcgg gctgcccccg ctcacgggcg ggttggtggg agccttgggt
     2161 tgggacatcg tccgccaatg ggagccgacc cttccggccg acgcagtgcg cgaactcgac
     2221 ctcccggatg cctatttgct gctcgcgact gacctcgccg tactcgacca cgttgacggc
     2281 tcggtgtggc tgattgcgaa cgcggtgaac accgacgccc aaccgaccgg tatcgatgag
     2341 gcgcatgcat cggcggtcgc gcgcgtcgat gc
//
LOCUS       DASRKH010000329         2568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_28798, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000329 DASRKH010000000
VERSION     DASRKH010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28798"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1118)
                     /locus_tag="VFC06_05260"
     CDS             complement(<1..1118)
                     /locus_tag="VFC06_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81336.1"
                     /translation="MNRLRARSAAIWAQVVRPFVWLDAASLRQQLVVGAGVYAVVLLA
                     LVATAINGTSSGQFYGFFFPGHDPRLLIGGPRTVRSDEWYVTTPLVISQVQQGFPRLS
                     DVFPGGVDVSIMWDVPYREWSTLFRPQNWGFFFLPLDNAFALRWWLPLVALATAAYAL
                     VVTLWRRPVAALALSAAFAFSPFTQWWFGSGTFWPLAFALLACAAVVWCLRTGSARLR
                     WAIASVTGYFAVVAVLTLYPPFLIPCVIVAAAFSVGWVVAAHELRWAERWRRLTPILV
                     AGVAAGVVVVVFLATRSQTVEAITSTVYPGNRRWPTGQPALFPWLSMYAGTFGVGLRA
                     ASVDGFAVNQSEGSSFLLVGFYLIPIALWLVWSRWRSH"
     gene            complement(1243..2088)
                     /gene="rfbD"
                     /locus_tag="VFC06_05265"
     CDS             complement(1243..2088)
                     /gene="rfbD"
                     /locus_tag="VFC06_05265"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HZL81337.1"
                     /translation="MRWLVTGSNGMLGQDLVQLLRAQGEDVVAVDRDSLDIIDPSAVD
                     AGVAGRDVIVNCAAWTAVDAAETQEGDAFAVNAVGPAHLARAASRHGARLVQISTDYV
                     FDGSATTPYAEDAPAAPRSAYGRTKMAGEWAVRAETSDYLIVRTAWLYGAGGPCFPKT
                     IARVAKERGGLDVVSDQVGQPTWTADLADLVVRLVTRGAPSGTYHGTSSGLTSWHGFA
                     QAIVASAGLDPASVRTTTSDAYASAAPRPGYSALGHDALVAAGVKPIGEWSARWADAA
                     RAVLL"
     gene            2181..>2568
                     /locus_tag="VFC06_05270"
     CDS             2181..>2568
                     /locus_tag="VFC06_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HZL81338.1"
                     /translation="MEFRQLKVPGAWEITPQQLGDPRGVFLEWFKASIFAETVGAPLD
                     LQQANCSVSAAGVLRGIHFADVPPGQAKYVTCAKGAVLDVAVDIRVGSPTFGVWDAVL
                     LDDVDRRAIYLPEGLGHAFLSLEDDST"
BASE COUNT          465 a          902 c          853 g          348 t
ORIGIN      
        1 ctgtggctgc gccaccggga ccagaccagc cacagagcga tcgggatcag gtagaaaccc
       61 acgagcagga agctcgaccc ctcgctctgg ttcaccgcga agccatcgac cgaggcggca
      121 cgcagcccga cgccgaaggt gcccgcgtac atggagagcc acgggaagag cgcaggctga
      181 cccgtcggcc agcgacggtt ccctggatac accgtggagg tgatggcctc gacggtctgg
      241 cttcgcgtcg ccaggaacac gaccaccacg actcccgccg cgactccggc gacaaggatc
      301 ggggtcaacc tccgccatcg ttcggcccac ctcagctcgt gagcggcaac gacccacccg
      361 accgagaatg cagcggcgac gatcacgcag ggaatgagga aggggggata cagcgtcagc
      421 acagcgacga ccgcgaagta tccagtgacc gatgcgatgg cccatctcag ccgcgcgctc
      481 cctgtccgca ggcaccacac gacggccgcg cacgccagca gcgcgaacgc caacggccag
      541 aaggttcccg agccgaacca ccattgcgtg aagggagaga aggcgaacgc cgcggagagg
      601 gcgagcgccg cgaccggtcg tcgccagagc gtcacgacca acgcatatgc cgccgtagcc
      661 agcgccacga gcggcagcca ccaccggagc gcgaacgcgt tgtccagcgg gaggaagaag
      721 aacccccagt tctggggccg gaagagtgtc gaccactccc gataagggac gtcccacatg
      781 atcgagacgt cgacgccccc ggggaacacg tcgctgagac gcgggaaccc ctgctgaacc
      841 tgcgagatca cgagcggggt ggtgacgtac cactcgtcgc ttcgcacggt gcgcggtccg
      901 ccgatgagga gccgagggtc gtgaccggga aagaagaatc cgtagaactg gcccgacgac
      961 gtgccgttga tcgccgtcgc cacgagcgca agcagcacca ccgcgtacac cccggcccca
     1021 accacgagct gctggcggag ggacgctgcg tccagccata caaacggtcg gaccacctga
     1081 gcccagatcg cagccgagcg tgcacgcaga cgattcatgc gggttgtcct ttggtctgac
     1141 cctcgggcgg cagcgtcgta gccggaccgc tcatgcggca gacatcgtga cacagcccgc
     1201 gccacaacga ggccctgctg aacctggggt cctgactctc cgtcagagaa ggacagcgcg
     1261 tgcggcgtcg gcccatcgag cggaccattc gccgatcggc ttcacgccag cagcgaccag
     1321 cgcatcgtgg ccgagcgcgg agtacccggg gcgcggggcg gcactggcat acgcgtcact
     1381 cgtcgtcgtg cgcaccgatg cagggtcgag cccggccgac gcaacgatcg cctgggcgaa
     1441 cccgtgccac gacgtcaggc ccgacgacgt gccgtggtag gtcccgctcg gagctccgcg
     1501 agtgacgagg cgtaccacga gatcggccag gtccgccgtc catgtcggct gcccgacctg
     1561 gtccgagacc acgtcgagcc ctccacgttc cttcgccacg cgagcgatcg tcttggggaa
     1621 gcaggggcca ccggctccgt acagccacgc cgtgcgcacg atgaggtagt cgctcgtctc
     1681 ggctcggacg gcccactcgc cggccatctt cgtgcggccg tacgcggatc gcggcgccgc
     1741 gggagcgtcc tccgcgtagg gggtcgtcgc agacccgtcg aagacgtagt cggtcgagat
     1801 ctgcacgagc cgcgccccgt ggcgtgatgc cgcacgagcg aggtgcgcgg gtcccacagc
     1861 attgacggcg aacgcgtcac cctcctgcgt ctccgctgcg tcgaccgcgg tccatgcggc
     1921 acagttcacg atgacgtccc gacccgcgac gccggcgtcc acggcggagg gatcgatgat
     1981 gtccagggag tcgcggtcaa ctgcgacgac gtcctcgccc tgcgcgcgca gcagctggac
     2041 caggtcctgg ccgagcatcc cgttcgaacc cgtgaccaac cagcgcacga cgaccccatc
     2101 cgtttgaatg ccaggagaag ccagcgtaac ggcgtcggta tcctgggacg tcgtcgtcgt
     2161 cacttcagag gagtaccacc gtggagttcc gtcagctcaa ggtgccggga gcctgggaga
     2221 tcacccctca gcagctcggc gacccccgcg gtgtgttcct cgagtggttc aaggcgtcga
     2281 tcttcgccga gactgtcggg gcacccctgg acctgcagca ggccaactgc tcggtctccg
     2341 cggccggcgt gcttcgcggc atccacttcg cggacgtccc gcccggtcag gcgaagtacg
     2401 tcacgtgcgc caagggcgct gtgctcgacg tcgcggtgga catccgggtc gggtcaccga
     2461 ccttcggcgt ctgggacgcc gtgcttctgg acgacgtcga ccggcgcgcc atctatcttc
     2521 cggagggtct gggccacgcc ttcctctcgc tcgaggacga ctcgaccg
//
LOCUS       DASRKH010000330         2308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_21296, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000330 DASRKH010000000
VERSION     DASRKH010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21296"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /locus_tag="VFC06_05275"
     CDS             complement(<1..974)
                     /locus_tag="VFC06_05275"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HZL81339.1"
                     /translation="MGILGTGIQDGLLLKPVKYQWAMDLYDQAVANTWFPNEIQLGED
                     LADFKKMTDEEKHAVTYLMSYFNPNELLVNKALAFGVYPYINAPECHLYLAKQMWEEA
                     NHCMSFEYVLETFPIDREAAYDSHVTVPSMRVKEEFEVKFIKRMTEETLDIKTTEGKK
                     DFIRNLISYNIILEGIWFYSGFMVALSFRQRNLLRNFGSLMDWVIRDESLHLQFGINL
                     ILTVLEENEDLQDAEFADEIRNMILGAVEMEEAYNRDLLPKGILGLNADYVNQYVKYL
                     ADRRLEELGFEAHYNVSNPAKWMATANDTLQLVNFFEATNTSYEVNGQT"
     gene            complement(1023..>2308)
                     /locus_tag="VFC06_05280"
     CDS             complement(1023..>2308)
                     /locus_tag="VFC06_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81340.1"
                     /translation="SADLARERGSYANFEGSGWSKGLVPFDTIAIAEADREVPIAVNR
                     TMRQDWDALRTKVAGGMRNATLMAVAPTASIGLVAGTTPGFDPQFSQIFSRATSSGKF
                     LEVNRNLVKDLQDLGMWEDVKDELLATQGDLSRIEGIPEHLQEVYKTSFQLSPYAFIE
                     VAARAQKWIDQAISRNIYLEDRDVENMMDLYAAAWERGVKTTYYLHVKPRHTAEQSTV
                     RVNKTEKINSTGSANGQGKGSSGPAKKGFGGFGGAKKGFGAATASANAPVADAVVQQA
                     VEAAPAEQAPAAAPSRRGFGGVATPAAPAAEAVSEPTPRTGGISIAPAAVMSQLAAEA
                     FAPAPERKPVVPPSGLPAIVEPAVVETAVVETAVVETAPPGGAEQFDSYQAPAGATVL
                     EVVETVYSADGDMEMVDGVACPVDPMERLQCESCQ"
BASE COUNT          375 a          714 c          767 g          452 t
ORIGIN      
        1 gtctgcccat tcacctcgta ggaggtgttg gtggcctcga agaagttcac gagctgcagc
       61 gtgtcgttgg ccgtcgccat ccacttggcg gggttcgaca cgttgtagtg ggcctcgaag
      121 ccgagctcct cgagacgacg atcagcaagg tacttgacgt actggttcac atagtcggcg
      181 ttgagaccca ggattccctt gggcagcagg tcgcggttgt aggcctcctc catctcgacg
      241 gcgcccagga tcatgttgcg gatctcgtcg gcgaactcag cgtcctgcag gtcctcgttc
      301 tcctcgagca ccgtgaggat caggttgatg ccgaactgca ggtgcagcga ctcgtccctg
      361 atgacccagt ccatgagtga accgaagttg cgcagcaggt tccgctggcg gaaactcagc
      421 gcgaccatga accccgagta gaaccagatc ccctcaagga tgatgttgta ggagatcagg
      481 ttgcggatga agtccttttt gccttcggtg gtcttgatgt cgagggtctc ttccgtcatc
      541 cgtttgatga acttgacctc gaactcctcc ttgaccctca tcgacggcac cgtgacgtgg
      601 gagtcgtagg cggcctcgcg gtcgatcggg aacgtctcaa ggacgtactc gaacgacatg
      661 cagtggttgg cctcctccca catctgcttg gccaggtaca ggtggcactc gggggcgttg
      721 atgtacgggt acacgccgaa ggcaagcgcc ttgttgacga gcaactcgtt cgggttgaag
      781 tacgacatca ggtaggtgac ggcgtgcttc tcttcgtccg tcatcttctt gaagtcggcc
      841 aggtcttccc ccaactggat ctcattgggg aaccacgtgt tggcgacggc ctggtcgtac
      901 aggtccatgg cccactggta cttgaccggc ttgagcagaa gtccgtcttg gattccggta
      961 ccgaggatgc ccatggtgtt actccttgtc gattgtcagg ctgagaaggg ggtggcgggt
     1021 ggctactggc aggactcgca ctggagtcgc tccatcgggt cgacggggca tgcgacgccg
     1081 tcgaccatct ccatgtcgcc atcggcggag tacacggttt ccaccacctc gagcacggtc
     1141 gcgcccgcgg gcgcttggta cgagtcgaac tgctcggccc cgcccggcgg tgccgtttcg
     1201 acgaccgccg tttcaacgac cgccgtttca acaacagcgg gctcgacgat cgcgggaagc
     1261 cccgagggcg gaacaacggg cttccgctcg ggcgccggag cgaaggcctc ggcggccaat
     1321 tgcgacatca ccgcagcggg cgcgatcgag atgccgcccg tccggggcgt gggctcggaa
     1381 acagcctcag ccgcgggagc ggccggcgtg gccaccccgc cgaatccgcg cctggaagga
     1441 gccgctgcgg gagcctgctc ggcaggggcc gcttcgaccg cctgctgcac caccgcgtcg
     1501 gcgacgggag cgttggccga tgcggtcgca gcgccgaagc ccttcttggc gccaccgaag
     1561 ccgccgaagc ccttcttggc gggaccggaa gagcccttgc cctggccgtt ggccgagccg
     1621 gtcgagttga tcttctccgt cttgttgacg cgcacggtgg actgctcggc cgtgtgacgc
     1681 ggcttcacgt gcaggtagta cgtggtcttc acgccacgtt cccaagcagc cgcgtacagg
     1741 tccatcatgt tctcgacgtc gcggtcctcg aggtagatgt tccggctgat ggcctggtcg
     1801 atccacttct gggcacgcgc cgcgacctcg atgaaggcgt acgggctgag ctggaacgag
     1861 gtcttgtaga cctcctgcag gtgctcgggg atgccctcga tgcgcgacag gtcgccctgc
     1921 gtggcgagga gttcgtcctt gacgtcttcc cacatgccca ggtcctgcag gtccttcacc
     1981 aggttgcggt tgacctcgag gaacttgcca gacgatgtcg cgcgggagaa gatctgcgag
     2041 aactgcgggt caaagcctgg cgtggtgcct gcgacgaggc cgatcgacgc ggtgggcgcg
     2101 acggccatga gggtcgcatt gcgcatgccg ccagcgacct tcgtacgcag ggcgtcccag
     2161 tcctggcgca tggtgcggtt gacggcgatc ggcacttcgc ggtccgcctc ggcgatcgcg
     2221 atcgtgtcga aggggacgag gcccttggac cagccggagc cctcgaagtt cgcgtacgag
     2281 ccgcgctcac gcgcaaggtc ggccgact
//
LOCUS       DASRKH010000331         2780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_10816, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000331 DASRKH010000000
VERSION     DASRKH010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10816"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2780
                     /gene="ileS"
                     /locus_tag="VFC06_05285"
     CDS             <1..>2780
                     /gene="ileS"
                     /locus_tag="VFC06_05285"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HZL81341.1"
                     /translation="DVVPRFETMRGKKVERRFGWDTHGLPAELEAERILGITDKSQID
                     EMGIAAFNKACRDSVLKYTDEWETYVTRQARWVDFENDYKTLDVTFMESVIWGFKQLY
                     DKGLAYEGYRVLPYCWRDETPLSNHELRMDDEVYADRQDPALTVLLPLIDAPGKLAGA
                     SLVIWTTTPWTLPSNLAAAVGPEIEYSVVVASEGAFVGQRLVVATARLGAYARELGDD
                     PRVVATVKGADLAGLRYEPPFDHFVGRENAHRILVADYVTTEDGTGIVHLAPAFGEED
                     MALCGVAGIEPVIPIDSKGRFTSEVPEYEGQQVFDANTPIIKDLKERGIVLRHETYNH
                     SYPHCWRCRNPLIYRAIGSWFVRVTEFRDRMVELNQQITWVPEHIKDGQFGHWLEGAR
                     DWSISRNRYWGTPIPVWVSDDPEHPRLDVYGSLEEIERDFGRLPLNEDGEPDLHRPYI
                     DDLTRPNPDDPTGASTMRRISDVLDVWFDSGSMPFAQVHYPFENQEWFDSHYPGDFIV
                     EYIGQTRGWFYTLHVLATAIFDRPSFKTCVSHGIVLGSDGRKMSKSLRNYPDVNEVFH
                     RDGSDAMRWFLMSSPILRGGNLMVTEEGIREGVRQVMLPMWSTYYFFTLYAGAANGGA
                     GVLGKAVDDARVATLPVMDRYVLAKTAELVRTVTEQMEGFDVPGASEALAQYMDLLTN
                     WYVRTQRERFWDEDADAFDTLYTVLETVTRLAAPLLPLETEEIWRGLTGGRSVHLTDY
                     PVAPAAWVDAFLGGAMDQVREVVSTAHALRKREQIRVRQPLAYLEAAVANPQAMWDHT
                     GLLMAELNVKSMVWSGLDEFTRKFPVERRLTVNARAAGPRIGKTVQQAIKGSKTGDWS
                     EIDGVVEFGGELRVERHESSAGQNGRRHRVVPLTADNSRELVLALFKSDPPGLDDAVD
                     LGPVARLGPFDR"
BASE COUNT          476 a          920 c          910 g          474 t
ORIGIN      
        1 aggacgtggt gccccgcttt gagacgatgc gcggcaagaa ggtcgagcgc cgcttcggct
       61 gggacacgca cgggctgccc gccgagctcg aggccgagcg cattcttggc atcaccgaca
      121 agtcacagat cgacgagatg ggtatcgccg cgttcaacaa ggcgtgccgc gactccgtcc
      181 tcaagtacac cgacgagtgg gaaacctacg tcacgcgcca ggcccgctgg gtcgacttcg
      241 agaacgacta caagacgctc gacgtcacgt tcatggagtc cgtgatctgg ggtttcaagc
      301 agttgtacga caagggcctc gcctacgagg gctaccgcgt actgccgtac tgctggcgcg
      361 acgagacgcc gctgtccaac cacgaactgc gcatggacga cgaggtctat gccgaccgcc
      421 aggaccctgc cctcacggtg ctgctcccgc tcatcgacgc gccaggcaag ctcgccgggg
      481 cgtcgctggt catctggacg acgacgccgt ggacgctgcc gtccaacctc gcagccgccg
      541 tcgggccgga gatcgagtac tcggtggtcg ttgcgtccga gggcgcattc gtggggcagc
      601 ggttggtggt cgcgaccgca cggctgggcg cttacgcacg cgaattgggc gacgacccta
      661 gggtggtcgc gacggtgaag ggcgccgacc tcgcgggcct gcgctacgag cctcctttcg
      721 accactttgt gggtcgcgag aatgcccacc gcatccttgt cgccgactac gtgacgaccg
      781 aggacggcac gggcatcgtc cacctcgccc ccgcgtttgg tgaggaggac atggcgttgt
      841 gcggggtcgc gggcatcgag ccggtcatcc ccatcgactc caagggccgg ttcacgtccg
      901 aggtgcccga gtacgagggc cagcaggtgt tcgacgccaa cacgccgatc atcaaggacc
      961 tcaaggagcg cggcatcgtg ctgcgccacg agacgtacaa ccacagctac ccgcactgct
     1021 ggcgctgccg caaccccctg atctaccgcg cgatcggctc ctggtttgtg cgcgtcaccg
     1081 agttccgcga ccgcatggtg gagctcaacc agcagatcac ctgggtgccc gagcacatca
     1141 aggacggcca gttcggtcac tggctcgaag gggcgcgcga ctggtctatc agccggaacc
     1201 gctactgggg cacgccgatc ccggtctggg tgagcgacga cccggagcac ccgcgtttgg
     1261 acgtgtacgg ctccctcgaa gagatcgagc gcgactttgg ccgcctgccg ctgaacgaag
     1321 acggcgagcc ggacctgcat cgtccgtata tcgacgacct cacgcgcccc aatcccgatg
     1381 accccaccgg ggcctcgacg atgcgccgca tctccgacgt gctcgacgtg tggttcgact
     1441 ccggatcgat gccgttcgca caggtgcact acccgttcga gaatcaggag tggttcgaca
     1501 gccactaccc tggcgacttc atcgtcgagt acatcggcca gacccgcggg tggttctaca
     1561 cgctgcacgt gctcgcgacc gcgatcttcg accgccccag cttcaagacg tgcgtgagcc
     1621 acggcatcgt gctgggctcc gacggccgca agatgtcgaa gtccctgcgc aactatccgg
     1681 acgtcaacga ggtgttccac cgcgacggct cggacgcgat gcggtggttc ctgatgtcat
     1741 cgccgatcct gcgtggcggc aacctcatgg tgaccgagga gggcattcgc gagggcgtgc
     1801 gccaggtcat gttgccgatg tggtcgacgt actacttctt cacgttgtac gcgggagcgg
     1861 ccaatggcgg ggcgggcgtg ctgggcaagg ccgtcgacga cgcgcgggtc gccactcttc
     1921 ccgtcatgga ccgctacgtg ttggccaaga ccgcggaact ggtgcggacc gtcacggagc
     1981 agatggaggg gtttgacgtt ccgggcgcct ccgaagcgct cgcgcagtac atggacctgt
     2041 tgacgaattg gtacgtgcgc acacagcgcg agcgcttctg ggacgaggac gccgacgctt
     2101 tcgacaccct gtacacggtg ctcgagaccg tgacgcgctt ggcagctccg ttgctgcctc
     2161 tcgagactga ggagatttgg cgcgggctca ccggcgggcg ttcggtgcac ctcaccgact
     2221 acccggtggc tcccgccgcg tgggtcgacg ccttcttggg cggcgcgatg gaccaggtgc
     2281 gcgaggtggt atccaccgct cacgcgctgc gcaagcgcga gcagattcgg gtgcggcagc
     2341 cgcttgccta tcttgaagcc gctgtcgcga atccgcaggc gatgtgggac cacacgggtc
     2401 tccttatggc agaactcaat gtcaagagca tggtgtggag cggtctcgac gagttcaccc
     2461 gcaagttccc tgtcgagcgc cgccttacag tcaatgcccg tgcagccggc ccgcgcatcg
     2521 gcaagacggt tcaacaagcg atcaaagggt ccaagacggg cgactggtcc gagatcgacg
     2581 gcgtcgtcga gttcgggggt gagctccgtg tcgagcgcca cgaatcctcc gccgggcaga
     2641 acggccgccg ccaccgcgtc gtccccttga ccgccgataa cagtcgtgag ctcgtactcg
     2701 ccctcttcaa gagcgatccc cccggactcg acgacgccgt cgatctcgga ccagtcgccc
     2761 gtcttggacc ctttgatcgc
//
LOCUS       DASRKH010000332         2380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_15735, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000332 DASRKH010000000
VERSION     DASRKH010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15735"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VFC06_05290"
     CDS             <1..304
                     /locus_tag="VFC06_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HZL81342.1"
                     /translation="LINGAAFGNHDALQIRADERVRLVMNNASMMFHPMHLHGHTFAL
                     AGTSGRPGIRKDTVNVLPMGTLAVDLQANNPGQWALHCHNAYHAELGMMTVLSYVA"
     gene            complement(851..2212)
                     /locus_tag="VFC06_05295"
     CDS             complement(851..2212)
                     /locus_tag="VFC06_05295"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HZL81343.1"
                     /translation="MSAPTYSELAELVSVLGQQLSALKEEVTALREENRALKARVGEL
                     EAQVRTNSRNSSKPPSSDGLGKPSPKSLRKASGRRPGGQAGHEGTTLRQVANPDVVVR
                     HEPRECGGCGADLADALVVGTQRAQVFDIPPIKVRVVEHQIIARRCGCGTVTRGRAPS
                     EVNAPAGYGPRAQAIMVYLFMGQFLSRERTAHALSDLFGAPVSPATVAAATTRAAGDL
                     VPFLSQVSTQIAAAAVAHFDETGLRCEGRLAWMHSASTDKFALLHAHANRGKTAMDDM
                     GVLPTFTGTAIHDAFAPYDRYTGATHSLCNSHLLRELAAVAEHHEQTDQTPGPDNWCW
                     ADQVTDALLTVLAAVDQANKSGSAAIDADVLATQTKLIRHGALIGANTTPANKVEQKH
                     RALARRILNRTDDYLRFATDLSVSFTNNAAEQQIRMVKIRQKVSGCMRTMKGAKDFAA
                     IRS"
BASE COUNT          374 a          755 c          817 g          434 t
ORIGIN      
        1 gctcatcaac ggcgccgcct tcggcaacca cgacgcgcta cagatccgtg ccgacgagcg
       61 cgtccggctg gtcatgaaca acgcctccat gatgttccac cccatgcacc tccacgggca
      121 caccttcgcg ctcgccggca cgagcggtcg cccgggcatc cgcaaggaca ctgtcaacgt
      181 gcttccgatg ggcacgctcg ccgtggacct gcaggcgaac aacccgggtc agtgggccct
      241 gcactgccac aacgcctacc atgccgagct cgggatgatg accgtcctgt cctacgtcgc
      301 atgagccgag tggcgccggt cgggccgacg agactcgtca aggacgtctc ttgctcgact
      361 tcgtcgatgg acgcgacggt cgtggtttcc cgagtcggcg gtcaggtagc gtcgatccag
      421 tgagcaagct gatctcgcaa tgctcgcaac tgaggcggtg gcgtccgccc gcgcccggta
      481 caccgccgct ggtagacggc tggacatcga agtcgcggga aaggcgccga tcgtgctggt
      541 tgaccgccaa cggctcgccg aagccctcgc gaacctgctc gacaacgcgt tgcctgcact
      601 atgtacgggt tcgggaccag tcccatcaca tccggttcat tgcgtctcta tggcgcggac
      661 gagcggtatg accttggtgc caagtatctg gatgctccgc agcatcgtgt cgtgcaccat
      721 ggctcggcgt agctggtcac gtggtcacgt ggtcacgtgg tcacgtggtg gcggggtacc
      781 aggggtcttg gctggtgagg cggttgagcg cgtcgaacat ggtgatgccg tgcttggcgg
      841 cggtcgcggt ctaggagcgg atggcggcga agtctttcgc gcctttcatg gtgcgcatgc
      901 agccggatac cttttgccga atcttgacca tgcggatctg ctgctctgcg gcgttgttcg
      961 tgaagctgac cgacaggtcg gtggcgaagc gtaggtagtc gtcggtgcgg ttgaggatcc
     1021 tgcgggcgag ggcccggtgt ttctgttcga ccttgttggc cggggtggtg ttcgccccga
     1081 tcagtgcgcc gtggcggatg agtttggttt gggtggccag gacgtcggcg tcgatggcag
     1141 cggacccgga tttgttggcc tggtcgacgg ccgccaggac cgtgagcagg gcgtcggtga
     1201 cttggtcggc ccagcaccag ttgtcgggac cgggggtctg atcggtctgc tcgtgatgct
     1261 cggccacggc ggcgagctcc ctcaacaggt gggagttgca caaggaatgg gtggcgccgg
     1321 tgtaccggtc gtagggggcg aacgcgtcgt ggatggcggt gccggtgaag gtgggcagga
     1381 cccccatgtc gtccatcgct gtcttcccac ggttcgcgtg ggcgtgcagc agcgcgaact
     1441 tgtcggtgga cgcggagtgc atccaggcaa ggcgcccctc gcagcgcagg ccggtctcgt
     1501 cgaagtgcgc gacggcggcg gcggcgatct gcgtgctgac ctgggacagg aatgggacga
     1561 ggtcgccggc cgcgcgggtg gtggccgcgg cgaccgtggc cggcgagact ggtgcgccga
     1621 acaggtccga cagggcgtgg gcggtccgct ctcgggacag gaactgtccc atgaacaggt
     1681 acaccatgat cgcttgggcc cgcggcccgt accccgccgg tgcgttcact tcactcgggg
     1741 ctcgtccgcg tgtgacggtc ccgcatccgc agcgccgcgc gatgatctgg tgctcgacca
     1801 cgcgcacctt gatcggcggg atgtcgaaca cctgcgcccg ctgggtgccc accaccaacg
     1861 catcggccag gtcggccccg cacccgccgc attcgcgcgg ttcgtggcgc accacgacgt
     1921 cggggttagc gacctggcgc aacgttgttc cctcgtgacc cgcctgcccg ccgggccggc
     1981 gtccagacgc cttgcgcagc gacttcgggg atggcttgcc cagaccgtcc gaggacggtg
     2041 gcttggagga gttccgcgag ttggtccgaa cctgggcctc gagctcaccg acgcgggcct
     2101 tgagcgcgcg gttctcctcg cgcaatgccg ttacttcttc tttcaatgcc gacagttgct
     2161 ggcccaacac cgagaccagt tccgcaagct cggaataggt cggtgcgctc ataaacgtat
     2221 tctcgcaccg tcgcacaatg aatcaggcgc gacacgccgc gcaattcaca cgcaaacgac
     2281 tcgaccaaaa tgatcgtatc caccgggctg gatatctaca tagaccgcaa cgaaaacctc
     2341 gcgcgagccg gcccgtcaca acctacgaca agctgaccag
//
LOCUS       DASRKH010000333         2240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_7749, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000333 DASRKH010000000
VERSION     DASRKH010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7749"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="VFC06_05300"
     CDS             complement(<1..509)
                     /locus_tag="VFC06_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81344.1"
                     /translation="MQKAITPALTRAHLTLGHDRIAGYAVRAEDVSFATTPAQLFEVH
                     GLGYPGTPFSPYAASIDILRFESSPHLQYRDVPGYIVPLWWLRHSRIPPGAELVRIFA
                     DGTSILLGRYGHVGTGWSVTQPGSHRPSLASLSRCVGPVAKWHGAYLEADVIDDDHTV
                     VLALANPPLS"
     gene            complement(798..2234)
                     /gene="purB"
                     /locus_tag="VFC06_05305"
     CDS             complement(798..2234)
                     /gene="purB"
                     /locus_tag="VFC06_05305"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013885107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HZL81345.1"
                     /translation="MGQPRVVLAQVEPAIALGPLDGRYRGAVAPLVDHLSEAALNRER
                     VHVEVEWLIHLTSHHVVPGAPVLSAEEQEYLRDVVATFGVAEIAELGAIEKKTVHDVK
                     AVEYFLKDRLAAAPSVLGTSTVLPAVSELVHFACTSEDINNLSYALMVRGGVEQVWLP
                     AARALVADLTALAHEHAGVPLLALTHGQPATPTTLGKEVAVLAHRLGRQLRRIERAEY
                     LGKLNGATGTFGAHVVAVPGADWPEVSRTFIEHLGLTWNPLTTQIESHDWQAELYSDV
                     ARFNRVLHNLSTDMWTYISNGVFIQIPVAGATGSSTMPHKVNPIRFENAEANLEISSA
                     LLDTLASTLITSRLQRDLTDSTTQRNIGIAFGHSLLAIDNVRRGLGGLSVDRAALERE
                     LDANWEVLGEPVQSAMRAAAVAGVTGMENPYERLKELTRGHRLTAADMRGFIAGLGLP
                     DDVAARLTALTPATYTGLAEQLVGYLED"
BASE COUNT          334 a          728 c          848 g          330 t
ORIGIN      
        1 gagagcggcg ggttggcgag cgcgaggacg acggtgtggt cgtcgtcgat gacgtcggcc
       61 tcgaggtacg cgccatgcca cttggcgacc ggcccgacgc atcgggacag cgacgcgagg
      121 gacggacggt gggacccggg ctgcgtcacg ctccagcctg tgccgacgtg gccgtaccgg
      181 ccgagcagga tcgaggtgcc gtccgcgaag atccgtacga gctcggcgcc cggcgggatg
      241 cgcgagtggc gcagccacca cagcggcacg atgtagccgg gcacgtcgcg gtactgcagg
      301 tgcggggacg actcgaagcg caggatgtcg atggacgcgg cgtacgggct gaagggtgtt
      361 ccggggtagc cgagaccgtg gacctcgaac agctgcgcag gcgtcgtggc gaacgagacg
      421 tcctcggcgc gcaccgcgta gccggcgatg cggtcgtgac cgagagtcag atgggctcgg
      481 gtcagcgccg gtgtgatcgc cttctgcatg agggtcacga tggcttcctc gccaaatcgg
      541 atgtaacgga ctaactggat cgggaccgat tgtgccggtc cgacagggcg tgtgtccagg
      601 agtcacccgt tcgtgggagg gccggacgcg cctccagagt cgagccgatc gtgatcgcga
      661 gcccctgacc aggggggacg catcgcggtg ccgatcatga gggacttatg agagcgtgca
      721 cagaatgggc tgttcgtgtc aggtgagaac actcccgacc ggccgggggt cggccgggac
      781 cggcccgcgt cggcgggtca gtcctcgagg tatccgacga gctgctcggc gaggcccgtg
      841 tacgtcgcgg gcgtcagggc ggtgagccgg gcggcgacgt cgtcgggcag gccgagcccc
      901 gcgatgaacc cgcgcatgtc cgcggcggtg agtcggtggc cgcgcgtgag ctccttgagg
      961 cgctcgtacg ggttctccat cccggtgacg ccggcgacgg cggctgcgcg catcgcggac
     1021 tgcacgggct cgcccagcac ctcccagttc gcgtcgagct cgcgctcgag cgcagcacgg
     1081 tccaccgaca gccccccgag cccgcggcgc acgttgtcga tcgcgagcag cgagtgaccg
     1141 aacgcgatgc cgatgttgcg ctgcgtcgtg gagtccgtga ggtcgcgctg caggcgggac
     1201 gtgatgaggg tcgaggcgag cgtgtcgagc agcgcgctcg agatctcgag gttcgcctcg
     1261 gcgttctcga accggatcgg gttgaccttg tgcggcatcg tcgaggagcc ggtggccccc
     1321 gcgacgggga tctggatgaa gaccccgttc gagatgtacg tccacatgtc ggtcgagagg
     1381 ttgtgcagga cgcggttgaa ccgcgccacg tccgagtaga gctcggcctg ccagtcgtgc
     1441 gactcgatct gcgtcgtgag cgggttccag gtcaggccga ggtgctcgat gaacgtccgg
     1501 ctcacctcgg gccagtcggc gcccggcacc gcgacgacgt gcgccccgaa cgtgccggtc
     1561 gcgccgttga gcttgccgag gtactcggcg cgctcgatcc ggcgcagctg gcggcccagg
     1621 cggtgcgcga ggaccgcgac ctccttgccg agcgtcgtcg gggtcgcggg ctgaccgtgc
     1681 gtgagcgcga gcagcgggac gcccgcgtgc tcgtgagcga gcgccgtgag gtccgcgacg
     1741 agcgcgcgcg cagccggcag ccacacctgc tcgacgccgc cacgcaccat gagcgcgtac
     1801 gacaggttgt tgatgtcctc gctcgtgcac gcgaagtgca cgagctcgct caccgccggg
     1861 agcaccgtcg aggtgcccag cacggacggt gccgccgcga ggcggtcctt gaggaagtac
     1921 tcgacggcct tgacgtcgtg gaccgtcttc ttctcgatcg ccccgagctc ggcgatctcg
     1981 gcgaccccga aggtcgccac gacgtcgcgc aggtactcct gctcctcggc ggacagcacg
     2041 ggtgcacccg gcaccacgtg gtgcgacgtg aggtggatga gccactcgac ctcgacgtgc
     2101 acgcgctcgc ggttgagcgc cgcctcggac aggtggtcga cgagcggcgc caccgccccg
     2161 cggtaccgac cgtcgagcgg cccgagcgcg atagccggtt cgacctgagc aagaacgacg
     2221 cgcggttgac ccatgggctc
//
LOCUS       DASRKH010000334         2927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_6087, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000334 DASRKH010000000
VERSION     DASRKH010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6087"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VFC06_05310"
     CDS             complement(<1..360)
                     /locus_tag="VFC06_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019619241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZL81346.1"
                     /translation="MSHPPTGPRRAATGGGMRGRGLTGGRDPDDIRAENAAAPAVAHL
                     WRRVGALFVPYRSQLALTAVLVLVTSAATVVPPLIVQRIFDHGLFPSDGAGGVAPPEL
                     GLLTKLVALMVAIFIASA"
     gene            complement(443..904)
                     /locus_tag="VFC06_05315"
     CDS             complement(443..904)
                     /locus_tag="VFC06_05315"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZL81347.1"
                     /translation="MPDVDAAAMATLVASRSLFGFVAQSLAPALDELTMPQFRVLVVL
                     SGAGPLRMGDLAERIGVHPSTLSRTVDRLVAGEWMERASAEESRREVHVKLSDRGRAL
                     VNDVTDRRRTQIVRVLTTLPEADRAAVRRGMELFAAAAGEAAPEDLLELGL"
     gene            1003..2766
                     /locus_tag="VFC06_05320"
     CDS             1003..2766
                     /locus_tag="VFC06_05320"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HZL81348.1"
                     /translation="MTQTVAIPWRHRMVARIRRSENSALLVLSVIIGAFAGLGAVAFR
                     WLIENATLLFSGHGDYSDAGHEAHPWLPWLGPFFIVLAPAVGGLIYGPLVHRFAKEAR
                     GHGVPEVMYAISKRGGRIPAKVAAVKALASAITIGSGGSVGREGPIVQIGAAFGSTLA
                     RWVRLNESQVRLLVACGAAGGIAATFNAPIAGVFFALELLLANFVARTFAPVVLASVT
                     ASIIGRAMLGNEPFLALPEFTVHSLREYALYAVLGLLAALVATSFTKILYWFEDAADK
                     IWKGPEWLRPGVGGLLLGLLLLAIPQMYGVGYPVLENAVMGKYLIGFLLLLLVAKIIA
                     TSLTIAIGGSGGVFAPSLFLGAALGAAFGQAADAMFPGLGISPGAYALVGMGAVFAGA
                     SRAPITAVIILFELTGEYSIILPLLMAVVISTTLSAAMSRESIYTAKLSRRGVDLLAR
                     NPRPELAGLKVSDLMLPAPKSVSTRMSLPDVLKALRASRRPGLPVLTEHGEYVGVVTA
                     VGAAAAVGDDDADTVTVSQLIEWRDYVAPDAPVEEVLPVLLSEDVYDGVPVLDAEGTV
                     VGWLRRDAVLRRVTAPKPAPK"
BASE COUNT          429 a         1034 c          957 g          507 t
ORIGIN      
        1 ggccgatgcg atgaagatcg ccaccatcag cgcgaccaac ttggtcagca gcccaagttc
       61 cgggggcgcg acacccccgg cgccgtcgga ggggaagagc ccatggtcga agatgcgttg
      121 cacgatgagc ggcgggacga ccgtggcggc cgaggtgacg agcaccagga ccgcagtgag
      181 agccaactgt gaccggtagg gaacgaacag cgcgccgacg cgtcgccaca ggtgcgccac
      241 ggcgggggct gcggcgtttt ctgcgcggat gtcatcgggg tcgcggccgc cggtgaggcc
      301 ccgaccgcgc atgccgcctc cggtggcggc gcggcgggga ccggtgggcg ggtgggacat
      361 gcggtttagc ctaggtggga gctttcccgc tcacgcaccc gattcgccgc aggcgtagcg
      421 ccgagggcgc agcgtcgtga ggctagagcc ccagttccag caggtcttcg ggcgctgcct
      481 cgccggcggc ggccgcgaac aactccatgc cgcgcctgac cgccgcgcga tccgcctccg
      541 gcaaggtggt gagcacccgc acgatctggg tgcgtcgccg gtccgtcacg tcgttcacga
      601 gcgcgcgccc gcggtcggac aacttcacgt gcacctcgcg acgactttcc tcggcgctgg
      661 ctctctccat ccactcgccc gccaccaggc ggtcgacggt gcgactcagt gtggacggat
      721 ggacgccgat gcgttccgcc aggtcgccca tgcggagggg gcccgcccca gacagcacca
      781 cgagcactcg gaactgcggc atcgtcagct catcgagcgc cggggccagc gattgtgcca
      841 caaagccgaa gagcgagcgc gaagcgacga gcgtggccat ggcggcggcg tcgacgtcgg
      901 gcacgtgagg attcttgtct ggcgcattca cgggtccacc gtagaccgat tcactgctaa
      961 tctacaacta ttgcattcgt acaactattg aggaaccact tcgtgactca gaccgtcgcc
     1021 atcccctggc gtcaccgcat ggtggcgcgc attcggcggt cggaaaactc cgctctgctg
     1081 gtgttgtccg tcatcatcgg cgcattcgca ggactcggag cagtggcctt ccgctggctc
     1141 atcgaaaacg cgaccctctt gttctccggc catggcgact actccgacgc cggccacgag
     1201 gcccacccat ggctgccgtg gctcggcccc ttcttcatcg tgctcgcccc cgccgtcggc
     1261 ggactgatct acggcccgct ggtgcaccga ttcgcaaagg aggcgcgcgg tcacggcgtc
     1321 cccgaagtca tgtacgcgat cagcaagcgc ggcggtcgca tccccgccaa ggtcgccgcc
     1381 gtcaaggctc tcgcttccgc gatcaccatc ggctcgggtg gctcggtcgg acgtgaaggc
     1441 cccatcgtgc aaatcggggc cgcgttcggc tccaccctcg cccgctgggt tcgactcaat
     1501 gagtcccagg tacgcctcct ggtcgcctgt ggcgccgcgg gcggcatcgc cgccacgttc
     1561 aacgccccca tcgcgggcgt gttctttgcg ctcgaactgc tgctcgccaa cttcgttgct
     1621 cgcaccttcg ctccggtcgt tctcgcgtca gtgaccgcct ccatcatcgg ccgcgcgatg
     1681 ctcggaaacg agccgttcct cgcccttccc gagttcaccg ttcactccct gcgcgagtac
     1741 gcgctgtacg cggtgctggg cctgcttgcc gccttggtcg cgactagctt caccaagatc
     1801 ttgtactggt tcgaggacgc tgcggacaag atctggaagg gacccgagtg gttgcgtccc
     1861 ggcgtcggcg gactgttgct tggcctgctg ttgctcgcga tcccgcagat gtacggcgtg
     1921 ggctatcccg tgctcgagaa cgcggtgatg ggcaagtacc tcatcggctt tttgctcctg
     1981 ctgttggtcg ccaagatcat cgccacctcg ctcacgatcg ctatcggcgg atcgggcggc
     2041 gtgttcgcgc cgtcgctgtt cctcggggcc gcgcttggcg ccgccttcgg gcaggccgcc
     2101 gatgccatgt tcccggggct cggcatcagc ccaggagcat atgccctggt aggcatgggt
     2161 gcggtgttcg ccggagcgtc tcgcgctccc atcaccgcag tcatcatcct gttcgaactc
     2221 accggcgaat actcgatcat cctgccgctc ctcatggccg tggtgatctc cacgaccctg
     2281 tccgcggcga tgtcccgcga gtcgatctac accgcaaagc tctcgcgccg cggcgtcgac
     2341 ctgctggcgc gcaacccgcg tccggaactc gcggggctca aggtgagcga cctcatgctg
     2401 cccgcaccca agtcggtcag cacacgcatg tcccttccgg acgtgttgaa ggccttgcgg
     2461 gcctcgcgcc gccccggcct gccagtcctc acggagcacg gcgagtacgt cggcgtcgtg
     2521 acggccgtcg gcgcggccgc ggcagtcggc gacgacgatg ccgacaccgt cacggtgtcc
     2581 cagctcatcg aatggcggga ctacgtcgcg cccgatgcgc ccgtcgagga agtgcttccc
     2641 gtgttgctca gcgaagacgt ctacgacggc gtgccggtgc tggacgccga gggcacggtc
     2701 gtgggatggc tgcgccgcga tgcggtcttg cggcgcgtca ccgcacccaa acccgccccc
     2761 aagtagcgca ccgagcgctt gcaggggtcg gactcgaccg attcgcgcgc cgcctcccgc
     2821 ccaccgcgcc acgagaacgc gcggtgacga tttggcaaaa gtccctggcg cgtcccgagg
     2881 cgccgttcta ccgttcgatc atgcgggcgt atcgcccaca tcggccg
//
LOCUS       DASRKH010000335         2382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_10838, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000335 DASRKH010000000
VERSION     DASRKH010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10838"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /gene="sufB"
                     /locus_tag="VFC06_05325"
     CDS             complement(<1..806)
                     /gene="sufB"
                     /locus_tag="VFC06_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HZL81349.1"
                     /translation="MTQDETIASLGNYEFGWHDEDAAGALAKRGLSEDVVRGISGLKN
                     EPEWMLKLRLKSLKLFGKKPMPTWGSDLTGIDFDNIKYFVRSTEKQATSWEDLPEDIR
                     RTYDRLGIPEAEKQRLVSGVAAQYESEVVYHQIREDLEQQGVIFMDTDTALREHPEFF
                     EEYFGTVIPAGDNKFASLNTAVWSGGSFVYVPPGVHVDIPLQAYFRINTENMGQFERT
                     LIIADEGSYVHYVEGCTAPIYKSDSLHSAVVEIIVKKGARVRYTTIQNWS"
     gene            complement(836..1483)
                     /locus_tag="VFC06_05330"
     CDS             complement(836..1483)
                     /locus_tag="VFC06_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HZL81350.1"
                     /translation="MATDTTRERILSLIASAGPITAATLAEKLGLTSAAVRRHLGALL
                     DDGLIADHDMPSPVDRGRGRPAKSFVATSVGQRTLQADYSDVAREAMSYLRESGDLPA
                     FVEAHAARLEASLAGAIDRDAPIAERVEALSEALAAQGYATSVRPGPGGHTLQLCQGH
                     CPVQEIAQVAPEWCEAEARAFSSVLNVHVQRLSTLAQGAHVCTTTIPLTPALKEG"
     gene            2146..>2382
                     /locus_tag="VFC06_05335"
     CDS             2146..>2382
                     /locus_tag="VFC06_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81351.1"
                     /translation="MTLGTSAVTFQECDPLGAGEDSHGAAEVQDFTPPAENLRNDACV
                     TGETPQLTDRYSLVGAVDTSQAAAGVEISAVDPHH"
BASE COUNT          363 a          746 c          835 g          438 t
ORIGIN      
        1 ttcgaccagt tctggatggt cgtgtagcgc acgcgggcgc ccttcttgac gatgatctcg
       61 accacggcgg agtgcagcga gtccgacttg tagatcggcg cggtgcagcc ctcgacgtag
      121 tgcacgtagg agccctcgtc ggcgatgatc agggtccgct cgaactggcc catgttctcc
      181 gtgttgatgc ggaagtaggc ctgcagcggg atatcgacgt ggacgcccgg ggggacgtag
      241 acgaacgatc cgccggacca cacggccgtg ttgagcgagg cgaacttgtt gtcgcctgcg
      301 gggatcacgg tgccgaagta ctcctcgaag aactcggggt gctccctcag cgcggtgtcg
      361 gtgtccatga agatgacgcc ctgctgctcg aggtcctcac ggatctggtg gtacaccacc
      421 tcggactcgt actgagccgc gacgcccgag acgaggcgct gcttctccgc ctccgggatg
      481 ccgagcctgt cgtacgtgcg cctgatgtcc tcgggcaggt cctcccagga ggtggcctgc
      541 ttctcggtgg agcgcacaaa gtacttgatg ttgtcgaagt cgatacccgt caggtctgac
      601 ccccacgtcg gcatcggctt cttgccgaac agcttgagcg acttgaggcg gagcttgagc
      661 atccactcgg gctcgttctt caggcccgag atgccgcgca cgacgtcctc gctgaggccg
      721 cgcttcgcga gcgcgcctgc cgcgtcttcg tcatgccaac caaactcgta gtttccaagg
      781 gacgcgatcg tctcgtcttg ggtcatgggg acgttgtcag tgggtgtgct catcgtcatc
      841 cttccttcag ggctggggtg agcgggatgg tggtggtaca cacgtgcgcc ccttgggcga
      901 gcgtggaaag tctctgtaca tgaacgttga gaactgagga aaatgccctg gcttccgctt
      961 cgcaccactc gggtgcgacc tgggcgatct cctgcacggg gcagtggccc tggcacaact
     1021 gaagggtgtg gccgccggga cccgggcgca ccgacgtcgc gtagccctgg gccgcgaggg
     1081 cctccgagag cgcctcgacc cgctcagcga tcggcgcgtc tcggtcaatg gcgcccgcga
     1141 gggacgcctc aagccgagcc gcgtgggcct ccacaaaggc tgggaggtct ccgctctctc
     1201 gcaagtagct catggcctcg cgtgccacat cggaatagtc ggcctggagg gtccgctggc
     1261 ccaccgaggt cgcgacaaag gatttggcgg ggcgtccgcg ccctcggtcc acggggctcg
     1321 gcatgtcgtg atcggcgatg aggccgtcat ccagcagcgc gccaaggtgg cgccttactg
     1381 cggccgacgt cagacccagc ttctcggcaa gggtggcggc ggtaatgggg ccggcggacg
     1441 cgatcaggct caggatgcgc tcgcgagtgg tgtctgtcgc cacgaggcct ccttccgacg
     1501 gtcgtgctgc tccgggcgcc ggggactcgg cgagagcact gtgttcctag agcactgtgg
     1561 ccgcgcgtgc tcgcggtcaa cgagtaatta aacaacagtg tcgtgccgaa aatgcccgag
     1621 cgcaagcaag ggaagcctta cttgactggg ggttgagcgc aggcgaccgc ggtttgcgcc
     1681 ctcgatgctc cgtgcagcag acccatgcgt cgcgcgcctt cggccccaag ccgggtggcg
     1741 ggcgcggggc gacgggcggt ggcagtgtca ggcggcagcg tcccaggcag agtcctgccc
     1801 cgttgcgtgg tcgcgggcag agtcctcccc cgttgcatga ctacgcgcag agtcctgccc
     1861 cgttgcgtgg ttacgcgcag agctctccct cggtacgggg ggctgcgcat cgtcggcgtt
     1921 gccgatttcg agtgcggcga gtcctcctgg tctgggtcgg ccggtggggt cgatggtgtg
     1981 tggcgggatg aagtcgacac ggcccgcttg gacgcggatg ccccagcctt ggcggtggac
     2041 gtcgtggtga cagcgagtgc ataacatgac gccgttgccg aggtccgtgg cgccaccgcg
     2101 ttcccaccag cggatgtggt gggcctcgca gtgttcaggc ggtgcgtgac acttggcaca
     2161 tccgccgtca cgttccagga gtgcgatcct ttgggcgcgg gtgaagattc gcacggtgcg
     2221 gccgaagtcc aggacttcac cccgcctgcc gagaacctcc ggaatgatgc ctgcgtcacc
     2281 ggcgagacgc ctcagctgac cgaccgatac agcttggtgg gtgccgtcga tacgtcccag
     2341 gccgcggccg gagtcgagat cagcgcggtc gatccgcacc ac
//
LOCUS       DASRKH010000336         2415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_1628, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000336 DASRKH010000000
VERSION     DASRKH010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1628"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..714)
                     /gene="upp"
                     /locus_tag="VFC06_05340"
     CDS             complement(79..714)
                     /gene="upp"
                     /locus_tag="VFC06_05340"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HZL81352.1"
                     /translation="MDLHVANHPLIDHKVTVLRDQTTPSPTFRRLVDELVTLLAYEAT
                     RHVRVEQRAVVTPLTTTTGTRIADPAPLIVPILRAGLGMLDGMTRLLPSAEVGFLGLK
                     RDEETLEAVTYANRLPEDLTGRQVFILDPMLATGGSLVAAIDYVLERGATDVTCVCLL
                     AAPEGIDHVRTHVGDRDGVNIVVACVDEKLNEVGYIVPGLGDAGDRLYGVV"
     gene            787..1236
                     /locus_tag="VFC06_05345"
     CDS             787..1236
                     /locus_tag="VFC06_05345"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013115732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HZL81353.1"
                     /translation="MDDAAAMREALAEARRALEHDDVPVGAVVLDPAGVIVGRGHNRR
                     EVDADPTAHAEVLALREAASEAGTWHLEGHTLVVTLEPCLMCAGALLAARIPRLVIGA
                     WDPKAGAAGSQWDVVRDARLAAQVEVVPGVDADAAAALLTEFFSDRR"
     gene            1322..1411
                     /locus_tag="VFC06_05350"
     tRNA            1322..1411
                     /locus_tag="VFC06_05350"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1356..1358,aa:Ser,seq:cga)
     gene            complement(1523..2269)
                     /locus_tag="VFC06_05355"
     CDS             complement(1523..2269)
                     /locus_tag="VFC06_05355"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HZL81354.1"
                     /translation="MSSRSRILRTRREVVRRHRRERQFLVFGLLIGLLATVSVIAYAI
                     YQGRIDPPIDYAFVTPPPDFESTIKLPCPPLDEGNDAADPEAVHENMPMPPSEVTVRV
                     LNGTKGTADEQSGLAGATLEVLKGRGYVGVGATNWSRTYSEYVRIQFGEEGLRQAYTV
                     AANFPNYVMVLDNRKGAVVDIILGDLFDTKKIRPQYAPELDRTVELTAPVPCLPINLV
                     PREPAPRIIPVDPLAPIVTPSPSPSPSPSA"
     gene            complement(2301..2388)
                     /locus_tag="VFC06_05360"
     tRNA            complement(2301..2388)
                     /locus_tag="VFC06_05360"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2352..2354),aa:Ser,seq:gga)
BASE COUNT          400 a          805 c          832 g          378 t
ORIGIN      
        1 tgacggagca gccctccgcg cagcagcgcg tcaacgactc ctatgtctcg gagtgcacca
       61 cgggctagcg gtccgccgct acaccacgcc gtagagccgg tcgcccgcgt cgccaagtcc
      121 gggcacgatg tacccgacct cgttgagctt ctcgtcgacg cacgcgacca cgatattcac
      181 gccgtcacgg tctcccacgt gcgtacgcac gtgatcgata ccttcgggcg cggccagcag
      241 gcagacgcac gtcacatcgg tggcgccgcg ttcaagcaca tagtcaatcg ccgcgacgag
      301 cgatcctccc gtggcgagca tcgggtcaag aatgaacacc tgacgtcccg tgaggtcctc
      361 ggggagccgg ttggcgtagg taacggcctc cagggtctcc tcgtcgcgtt tgagcccgag
      421 gaacccgacc tcggcgctgg gcaacaaccg cgtcatccca tccagcattc ctagccccgc
      481 ccgcaagatg gggacgatga gcggcgcagg atccgcgata cgggtgcctg tggtggtggt
      541 cagaggagtg acgacggcgc gctgctcgac gcgaacgtgg cgcgtggcct catacgcgag
      601 cagggtcacg agttcgtcga ccaaccggcg aaacgtcggt gacggagtgg tctggtcccg
      661 cagcacggtg accttgtggt cgatgagggg gtgattcgcg acgtgaagat ccatgggcat
      721 cacggtaccg ctaccgcggg cccctcagca gtcatcgcaa acgccaagtg ggctagcgtg
      781 gcgcccatgg acgatgccgc ggcaatgcga gaggccctcg ccgaggcccg gcgagccctg
      841 gagcacgacg acgttcccgt cggcgccgtc gtcctcgacc ctgcgggcgt catcgtcggc
      901 cgtgggcaca accgccgtga ggtcgacgca gaccctacgg cgcacgccga ggtcctggcg
      961 ctgcgggagg ccgcgtccga ggccgggacg tggcacctcg agggccacac gctggtggtc
     1021 acgctcgagc cgtgcctgat gtgcgcgggc gcactcctcg cagcccgcat cccgcggctg
     1081 gtgatcggcg cgtgggaccc caaggcgggg gctgccggca gtcaatggga cgtggttcgt
     1141 gatgcccgcc ttgcggcgca ggtcgaggtg gttcccggcg tcgacgcgga tgccgctgcc
     1201 gcgctgttga ccgagttctt ctccgaccgc cggtagccag cgcgacccgc cggccaccgc
     1261 gccttgagac agcgggcacc ccggtttggt gggcacccgt cggcccggta tccttgtccg
     1321 cggtgccgtg tccgagcggc ctaaggagca cgcctcgaaa gcgtgtgtgg gtgagagtcc
     1381 accgtgggtt caaatcccac cggcaccgcc aagagcaacg gcccggtctc ctcgagggga
     1441 gccgggccga tgccgtttcg gggcccctct cggggcgggc ggcgcgaggt gccgtagggt
     1501 gcggcgcgca gggccgtggg gcctaagccg aaggagacgg cgacggagac ggactgggcg
     1561 tgacgatagg cgccaaaggg tccacgggaa tgatgcgcgg cgcaggctcc cgcggcacca
     1621 agttgattgg caagcacggt accggcgccg tcaactccac ggtgcggtcg agctcgggcg
     1681 cgtactgcgg gcggatcttc ttggtatcga acaggtcgcc cagaatgatg tcgacgactg
     1741 cacccttgcg gttgtccagc accatcacat agttcgggaa gttggcggcc actgtgtacg
     1801 cctggcgcaa gccctcctcg ccaaactgga tgcgcacgta ctcgctgtac gtacgactcc
     1861 agttggtggc gcccacaccc acgtagccgc gacccttcag cacctcaagc gtcgcgcccg
     1921 cgagacccga ctgctcgtcg gccgtcccct tggtgccgtt cagcaccctc accgtgacct
     1981 cgctcggcgg catgggcatg ttttcgtgaa cggcctcggg gtcggccgcg tcgttccctt
     2041 catccagcgg cgggcatggc aacttgatgg tcgactcgaa gtctggcggc ggggtcacaa
     2101 aggcgtagtc gatcggcggg tcgatgcgcc cttggtagat cgcgtacgca atcacggaaa
     2161 cggtggcgag aaggccgatc aacagtccga acacgaggaa ctggcgttcc ctccggtgac
     2221 ggcgcaccac ctcgcgtcgc gtgcgcaaga tgcgggagcg agacgacaca gtcacctacc
     2281 tcgggttcgg acgggtcaag tggcggagga tgggggattc gaacccccga gggcttgcac
     2341 ccaacacgct ttccaagcgt gcgccatagg ccactaggcg aatcctccag gcccaagcat
     2401 gttacccgag cggcg
//
LOCUS       DASRKH010000337         2163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_11022, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000337 DASRKH010000000
VERSION     DASRKH010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11022"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VFC06_05365"
     CDS             <1..191
                     /locus_tag="VFC06_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HZL81355.1"
                     /translation="APEPRRGFGAVKVEVTVGETTWRTSVFPSKEAATFVMPVKRAVL
                     KAEGLTVGDSVDFVLTTV"
     gene            complement(198..1049)
                     /locus_tag="VFC06_05370"
     CDS             complement(198..1049)
                     /locus_tag="VFC06_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HZL81356.1"
                     /translation="MEPIDRRWVAGRWPVPAGSDDKYSRGVLGIVAGSEAYPGAAALV
                     ASAAVRAGIGLVRYVGPRRAQDLVLSMRPEVVVHDPGEATQRLPRVSAWVIGPGVADH
                     PGQDAVIGAVLASGVPLVVDAGALEACARARAAGDRQAPPDRVLMTPHERELVATLGA
                     MRHDITAEDVARDRQAHARLLAETARATVLLKGSPTLVAPPGGAVVTLPDAPAWLATA
                     GSGDVLAGIAGALLASGLSALEAGVAASWIHARAAEAASGGGPIAALDIADALPAVVA
                     ELLLEGS"
     gene            1129..1623
                     /gene="def"
                     /locus_tag="VFC06_05375"
                     /pseudo
     CDS             1129..1623
                     /gene="def"
                     /locus_tag="VFC06_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136866.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
     gene            complement(1735..>2163)
                     /locus_tag="VFC06_05380"
     CDS             complement(1735..>2163)
                     /locus_tag="VFC06_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HZL81357.1"
                     /translation="TIDDRVRSVIEDMLETVDHEGRAGLAANQIGVSLRAFSWNIDGD
                     VGYVLNPEIVENHSEYQELGDEGCLSVPGLWYPCERPLYAKAVGTDLDGNEVVVEGEE
                     IWARLVNHEVDHLNGKLYIDRLSKDVRRSAMRELREQMER"
BASE COUNT          370 a          751 c          726 g          316 t
ORIGIN      
        1 ccgcgcccga accccgcagg ggattcggcg cagtcaaggt cgaggtcaca gttggcgaga
       61 ccacgtggcg cacctccgtc ttcccctcga aggaggcggc caccttcgtg atgcccgtca
      121 agcgggcggt gctgaaggcg gagggcctga ccgtgggcga cagcgtggac ttcgtcctga
      181 cgaccgtcta gcgcccgcta ggaaccctca aggaggagtt cggcgaccac ggcgggcagc
      241 gcatcggcga tgtccaaggc cgcgatcggg ccgcccccgg acgcggcttc cgcggccctc
      301 gcatggatcc acgacgccgc cacgcccgcc tccagggcgc tgaggccgga agccagcaac
      361 gcgcctgcga tgccagcgag cacatcgccg gaacccgcag tcgcgagcca cgccggcgca
      421 tccggcaggg tcaccactgc tccgcccggg ggagcgacca gcgtggggga acccttgagg
      481 agcacggtcg cgcgggcggt ctcggcgagc agccgcgcgt gggcctgcct gtcgcgggcc
      541 acatcctctg ccgtgatgtc gtggcgcatc gcgccgagcg tggccaccag ttcccgctcg
      601 tgaggcgtca tcagcacccg gtcaggaggc gcctggcggt ctccagcggc ccgcgcacgc
      661 gcgcaagcct ccagggcacc cgcgtcgacc acgagcggca cacccgaggc gagaacggcg
      721 ccgatgacgg catcctgccc tggatgatcg gcgacgcccg gcccgatgac ccacgcggac
      781 acccgcggca gtcgctgggt cgcctcgcca gggtcatgca cgaccacctc gggcctcatg
      841 gacaacacga ggtcttgggc ccggcgaggc cccacgtagc ggaccaggcc gataccggcg
      901 cgcacggcgg ccgatgccac gagcgcggcc gcgcccgggt acgcctcgga gccggccacg
      961 atgcccagca cgccgcgaga gtacttgtcg tcactacctg cggggacggg ccagcggccc
     1021 gccacccaac gcctgtcgat gggctccatg gccctagcct ggcacgggac gccccctgag
     1081 gtcgtccctc ctgcaagagg cccctcagaa catgacagaa ttgcgcccat ggcaatgcga
     1141 gagattcggg tagtcggcga cccagtactg cgtaccccct gtgaacccat caccaccatc
     1201 gacgaccgtg tgcgctcggt catagaagac atgttggaga cggtcgacca cgagggccgt
     1261 gcggggctgg ccgccaacca gattggggtc tcgctgcgcg ccttctcctg gaacatcgac
     1321 ggcgacgtgg gatatgtgct caacccggaa attgtggaga atcacgcgga gtaccaggaa
     1381 ctcggcgatg aaggctgcct ctaggttccc ggcctgtggt acccgtgtga gcggcctttg
     1441 tatgcgaagg ctgtgggaac ggacctcgac gggaacgagg tcgtcgtcga gggcgaggag
     1501 atctgggccc gactcgtcaa tcacgaggtc gaccacctga acgggaagtt gtatctcgac
     1561 cgactgagca aggaagtgcg tcgcagcgcc atgcgcgagc tccgcgagca gatggagagc
     1621 tagccaatcg gctagatgcc cacgtcgacc acgacgactt cgcccggtgt tgccagaggc
     1681 gtgccacaaa gcgggcgaag tcgtcgtggt cgacgtgggc atctagccga ctggctagcg
     1741 ctccatctgc tcgcggagct cgcgcatggc gctgcgacgc acgtccttgc tcagccggtc
     1801 gatgtagagc ttcccgttca ggtggtccac ctcgtggttg acgaggcggg cccagatctc
     1861 ctcgccctca acgacgacct cgttcccgtc gaggtccgtc cccacggcct tcgcgtagag
     1921 gggccgctcg caggggtacc acaggccggg aaccgagagg cagccttcat cgccgagttc
     1981 ctgatactcc gagtggttct cgacgatctc cggattgagc acatacccca cgtcgccgtc
     2041 gatgttccag gagaaggcgc gcagcgagac cccaatctgg ttggcggcca gccccgcacg
     2101 gccctcgtgg tcgaccgtct ccaacatgtc ttctatgacc gagcgcacac ggtcgtcgat
     2161 ggt
//
LOCUS       DASRKH010000338         2192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_10947, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000338 DASRKH010000000
VERSION     DASRKH010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10947"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="VFC06_05385"
     CDS             <1..741
                     /locus_tag="VFC06_05385"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HZL81358.1"
                     /translation="AALAARGDSRAAAALERQRWWFLRFSPRDDEGVVWHPRDSSEVW
                     VDSIYMVVPMLVLTGDVAPADMQYRMHREHLWDERSGLYGHRSDTVTGQRVRGAFWAS
                     GNGWAAGGIARALRIGGDGVPDEMRGRWRSNTQDLLDAAAAHETTDGRFHDVLDDPAT
                     FTDGAAGLMLAYAAFTGVADGWLPAEYAGHATRWLEGALARVEADGVVQGVCGAPHFD
                     REGVSAEAQAFAIMAIAAFERASRGSSV"
     gene            complement(738..1373)
                     /gene="serB"
                     /locus_tag="VFC06_05390"
     CDS             complement(738..1373)
                     /gene="serB"
                     /locus_tag="VFC06_05390"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HZL81359.1"
                     /translation="MSPRRLLVLDVDSTLIRQEVIELLAAHAGSQAHVAAITERAMRG
                     EIDFESSLAERVATLKGLSTSVFHEVAAEVEFTPGAPELVEAAQAAGWTVALVSGGFE
                     EIVSSIASAVSVSLFVANRLEVDQGHLTGRTVGPVIGRNAKAVALAAFARGAGIPITD
                     TVAIGDGANDLGMMAAAGLSVAFAAKPVVRAAADISIEGPRLDAAWPLISS"
     gene            complement(1370..>2192)
                     /locus_tag="VFC06_05395"
     CDS             complement(1370..>2192)
                     /locus_tag="VFC06_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HZL81360.1"
                     /translation="IESGADFTVAGIRQPIEMADQFGVVDVDPDNTTRVRAFLEKPTA
                     PDGLPDSPTEILASMGNYVASVEPLLEALMADAASETSKHDMGGDIVPYFVDKGTCGL
                     YDFVQNDIPGSTTRDKDYWRDVGTIDSYYDAHMDLIAVMPVFNVYNSEWPLHQGLITH
                     PPAKFIHSEEGRMGHAADSVVSPGVIVSGATVTGSILSPGVRLHSWSTVSDSVLLDDV
                     TVGRHAQVHRAILDKHVVVEEGARVGIDHEADIARGFTVTESGLTVVAKGTVIHG"
BASE COUNT          360 a          705 c          759 g          368 t
ORIGIN      
        1 gccgcgttgg cggcccgggg cgattcacgg gctgcagcgg ctctcgaacg ccaacgctgg
       61 tggttcctgc ggttctcccc acgggacgac gagggcgtcg tctggcatcc gcgagattcc
      121 agcgaggtgt gggtggactc gatctacatg gtggtgccga tgctggtgct cacgggtgac
      181 gttgcgccag ccgacatgca gtaccggatg cacagggaac acctgtggga cgagcgcagc
      241 gggctctacg gacaccgcag cgacaccgtg acgggtcagc gcgtgagggg cgcgttctgg
      301 gcctccggaa acgggtgggc agcgggaggt attgccaggg cgttgcgcat cggcggggac
      361 ggtgtgcctg atgagatgcg ggggcgttgg cgcagcaaca cccaagacct cttggacgcc
      421 gcggcggccc acgagaccac cgacggccgg ttccacgacg tgctggacga tcccgcgacc
      481 ttcacggatg gcgccgcggg cctcatgctc gcctacgcgg cgttcacggg ggtggccgac
      541 gggtggctgc ccgctgagta cgcgggccac gccacgcggt ggcttgaggg ggctctcgcg
      601 cgagtggaag ccgacggcgt cgttcagggg gtgtgcggag ccccgcactt tgaccgcgaa
      661 ggcgtgtcgg ccgaggccca agcgttcgcg atcatggcca tcgcggcttt cgaacgcgcc
      721 tcacgaggct cgtccgtcta ggacgagatc aggggccacg cggcgtcgag gcgaggaccc
      781 tcaatcgaga tgtcggcggc ggcccgcacc acgggcttcg ccgcaaaggc gactgatagc
      841 cccgcggcgg ccatcatccc caggtcgttt gcaccgtcgc caatcgcgac cgtgtcggtg
      901 atcggtatgc ctgcccccct ggcgaatgcg gcaagagcca cggccttggc gttgcggccg
      961 atcacgggcc ccaccgtgcg ccccgtcagg tgaccttggt ccacttcgag gcggttggcc
     1021 acaaacaagc tgacggacac ggcgctcgcg atcgaagaca cgatctcttc gaagcctcct
     1081 gagacgagcg ccaccgtcca gccagccgcc tgtgcggcct cgacgagttc cggggcgccg
     1141 ggcgtgaact cgacctctgc cgccacctcg tgaaacaccg acgtcgagag gcccttcagg
     1201 gtggcgaccc tctcggcaag cgacgactcg aagtcgatct cgccgcgcat ggctcgttcg
     1261 gtgatggccg cgacgtgagc ctgtgagccg gcatgggccg cgagcaactc gatcacttct
     1321 tgcctgatga gagtggaatc gacgtccaag acaagcagac gccttgggct catccgtgga
     1381 tcacggtgcc cttcgctacg acagtcaggc ccgactcggt gacagtgaag cctcgagcga
     1441 tgtcggcctc gtggtcgatg cccacgcggg caccttcctc gacgacgacg tgcttgtcca
     1501 agatggcgcg gtgtacttgc gcgtgacggc ccaccgtgac gtcgtcgagc agcaccgagt
     1561 cagacaccgt ggaccacgag tgcaaccgca cccctggcga caggatcgaa cccgtcacgg
     1621 tcgcgccgga cacgatgact cctggcgaca ccactgagtc ggccgcatgg cccatgcgcc
     1681 cctcctcgga gtgaatgaac ttggcgggcg gatgcgtgat gagcccttgg tgaagcggcc
     1741 attcgctgtt gtatacgttg aagacgggca tcacggcgat caggtccatg tgggcgtcgt
     1801 agtacgagtc gatcgttccc acgtcgcgcc agtagtcctt gtcccgggta gtagagccgg
     1861 gaatgtcatt ctggacaaag tcgtacagtc cgcatgtgcc cttgtccacg aagtagggga
     1921 cgatgtcgcc gcccatgtcg tgcttggacg tctcgcttgc ggcgtcggcc atgagggcct
     1981 cgagcaacgg ctcgacggac gccacgtagt tgcccatcga ggcgaggatc tcggtcgggc
     2041 tgtcgggaag gccgtctggt gccgtcggct tctccaagaa agcgcgcacg cgagtcgtgt
     2101 tgtcggggtc cacgtcgacc acgccaaact ggtccgccat ctcgatcggt tggcggattc
     2161 ctgcgaccgt gaagtcggcg cccgactcga tg
//
LOCUS       DASRKH010000339         2142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_58806, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000339 DASRKH010000000
VERSION     DASRKH010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58806"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="VFC06_05400"
     CDS             complement(<1..855)
                     /locus_tag="VFC06_05400"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013885100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HZL81361.1"
                     /translation="MTQVPLRPAVAALPAYVPGGRSPVGAAAFKLSSNENPFPPLPSV
                     VAAIIDGAMDVNRYPDMYATELTEAIATRWGVPPASLVVGNGSIAVLGHVLAAVVDAG
                     DEVVYPWRSFEAYPIAIAVAGGVGVPVPVTDDGRLDLAAMAAAVTPRTRVVLVCTPNN
                     PTGPAVHADEFSDLLAAVPRDVLVVLDEAYVEFVRDPLAADALAVFAGRPNVVVLRTF
                     SKAYGLAGLRVGFAVARPRLAAGIRATSTPFGVSHVAQLAAIASLRAADELLARVDAI
                     VDERVRMLA"
     gene            923..1306
                     /locus_tag="VFC06_05405"
     CDS             923..1306
                     /locus_tag="VFC06_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014923114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HZL81362.1"
                     /translation="MGFLMRVIINGVAIWLATVLLSGLQIVGADSTGQKIGIILLIAL
                     IFGLVNAIVKPLVKILSFPLYVLTLGLFTLVVNALMLWLTAWITGHTDWGLRVDSFGT
                     AVLGALIVSVVSFVLSVALPGSRKR"
     gene            complement(1322..>2142)
                     /locus_tag="VFC06_05410"
     CDS             complement(1322..>2142)
                     /locus_tag="VFC06_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81363.1"
                     /translation="LLPRRPLLVVPVLGSTRTLTGTDVPASDGDALRSVRDGYATPGA
                     VAVTRLDHPDGTRSWLVAVPGTEEGTVGGPNPLDMQSNLELLAGQSAAASELTVAAME
                     QAGIAPGEPVMLAGHSQGGMTAMALAGSAAVTARFAITHVLTAGSPVAGMSTQPGIQV
                     LNVEHTTDVIPTLDGRTNPDTRDRTTVRRDLSVSDDPAERAARLSLVDAHDVDLYVRT
                     LDLVDVSAAQDASINAWQESVGSAVFGRDGTTAVVTEYAGIGDVEVATLSPATR"
BASE COUNT          300 a          777 c          763 g          302 t
ORIGIN      
        1 ggcgagcatc cggacgcgtt cgtcgacgat cgcgtcgacc cgcgccagca gctcgtcggc
       61 cgcacgcagc gatgcgatgg ctgcgagctg cgcgacgtgc gacaccccga acggggtcga
      121 ggtcgcacgg atgcccgcgg ccagacgcgg tcgtgcgacc gcgaagccga cgcgcaggcc
      181 cgcgagcccg tacgccttgg agaacgtccg cagcaccacg acgttcggcc ggcccgcgaa
      241 caccgcgagc gcatcggccg cgagcgggtc gcggacgaat tccacgtagg cctcgtcgag
      301 gacgacgagc acgtcccgcg ggaccgcggc gaggaggtcg gaaaactcgt cggcgtgcac
      361 cgccgggccg gtcgggttgt tcggggtgca caccagcacc acccgggtgc gcggggtgac
      421 cgccgcggcc atggcggcca ggtcgagccg gccgtcgtcg gtcacgggca ccggcacgcc
      481 gacaccgccc gcgacggcga tcgcgatcgg gtacgcctcg aacgaacgcc acgggtagac
      541 gacctcgtca cccgcgtcga cgactgcggc gagcacgtgc ccgagcaccg cgatcgagcc
      601 gttgcccacg acgaggctcg cggggggcac accccaccgg gtcgcgatcg cctcggtcag
      661 ctcggtcgcg tacatgtcgg ggtagcggtt gacgtccatc gcgccgtcga tgatcgcggc
      721 gaccaccgac ggcagcgggg ggaacgggtt ctcgttggac gacagcttga acgcggccgc
      781 tccgacgggg gatcgaccac ccgggacgta cgcaggaaga gcagcgacag ccggccggag
      841 aggtacctgg gtcacacgac cagcatgcca ttgcccgacg attcccctgc gagcgcgggg
      901 cgtcgactgg caggatcacc tcatgggctt cctcatgaga gtcatcatca acggcgtcgc
      961 gatctggctc gcgaccgtcc tcctgtccgg cctgcagatc gtcggcgccg actccacggg
     1021 tcagaagatc ggcatcatcc tgctgatcgc gctcatcttc ggtctcgtca acgcgatcgt
     1081 gaagccgctc gtcaagatcc tctcgttccc gctgtacgtc ctcacgctcg ggctgttcac
     1141 gctggtcgtc aacgcgctca tgctctggct gaccgcgtgg atcacgggtc acaccgactg
     1201 gggcctgcgc gtcgactcct tcggcaccgc ggtgctcggg gcgctcatcg tctcggtcgt
     1261 gagcttcgtg ctcagcgtcg cgctgccggg tagccgcaag cgctgagcac cgcaccggcg
     1321 ttcaccgggt cgccggcgac agcgtcgcca cctcgacgtc tccgatgccc gcgtactcgg
     1381 tcaccacggc cgtcgtgccg tcgcgcccga agaccgccga cccgacggac tcctgccacg
     1441 cgttgatcga cgcgtcctgg gccgccgaga cgtccaccag gtcgagcgtc cgcacgtaga
     1501 ggtcgacgtc gtgcgcgtcg acgagtgaca gccgggcggc acgctcggcc gggtcgtcgg
     1561 acacggacag gtcccgtcgc acggtcgtcc ggtcccgcgt gtccgggttc gtgcggccgt
     1621 ccagcgtggg gatcacgtcg gtcgtgtgct cgacgttgag gacctggatg ccgggctgcg
     1681 tgctcatccc ggcgaccggc gatccggcgg tgagcacgtg cgtgatcgcg aaccgggccg
     1741 tcacggcggc cgacccggcg agtgccatcg ccgtcatgcc gccctggctg tggcccgcga
     1801 gcatgaccgg ttcgcccggc gcgatgcctg cctgctccat cgctgcgaca gtcagctcgc
     1861 tcgcggccgc ggactgcccg gcgagcagct cgaggttgct ctgcatgtcg agggggttcg
     1921 gcccgccgac cgtcccctcc tcggtccccg gcacggcgac gagccacgag cgcgtcccgt
     1981 ccgggtggtc gagccgcgtc accgcgaccg cgccgggcgt ggcgtagccg tcgcggaccg
     2041 accgcagcgc atcgccgtcg gacgccggga cgtcggttcc cgtcagggtc cgggtgctgc
     2101 cgagcaccgg gaccacgagc agggggcgcc gcggcaggag gg
//
LOCUS       DASRKH010000340         3006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_52820, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000340 DASRKH010000000
VERSION     DASRKH010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52820"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1502
                     /locus_tag="VFC06_05415"
     CDS             <1..1502
                     /locus_tag="VFC06_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HZL81364.1"
                     /translation="NATHQMKKMKVKDLKALRDLLHIPITDEQLEADPYLPPYFKPSD
                     DDPALQYMLERRKTLGGFLPERKAPKLNSLTLPGDKPYEILKGGSGQQEVATTMAFVR
                     LLKELIKDKEFGRRIVPIIPDEARTFGLDSIFPTAKIFNTAGMNYTAVDHDLMLSYKE
                     SEQGQILHLGINEAGSAAALQVVGTTYATQGEPMVPVYIFYSMFGFQRTGDQFWSAGD
                     QLARGFVIGATAGRTTLAGEGTQHMDGHSHILAATNHAFVCYDPAYAYEIRHIVRDGL
                     QRMYGQDESRDQNVMYYLTVYNEPMQQPAEPDDVDVEGILKGIHRVADHDHAGGSKVQ
                     LLASGVGLPWAMHAKELLRDDWGVDAAVWSVTSWNELRRDGLEVDKHNFIHPEEEKQV
                     AFVTRKLAETDGPVISTSDWDHMVQDQIRPWVPRPYYTLGADGFGFSDTRAAARRHFL
                     IDSHSMATRALQALVEAGQYDGGAVAHAIAKYDLFNVNAGRSGSVGGDA"
     gene            complement(1588..2295)
                     /locus_tag="VFC06_05420"
     CDS             complement(1588..2295)
                     /locus_tag="VFC06_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81365.1"
                     /translation="MAYEATRRAALRGASRPPGSSGAAGGVIVMGMGILDRIKAKIES
                     AQAQNDAAEEAALLARLEADPNDREALLDLADALTAPVPDEIVDPLTATQAPATLADQ
                     HNTALWALAEEYAGNPHAWAPLIELARLVVVDDPDGAARRLATACDRETTGRALAQSV
                     QMLRDHGLAHQAVSLGVAKWTPAVHEFATGRQLVLAAVEAKDEIKARELVSRLGDAHP
                     HDPAVTELEQLVEALEA"
     gene            2328..>3006
                     /locus_tag="VFC06_05425"
     CDS             2328..>3006
                     /locus_tag="VFC06_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HZL81366.1"
                     /translation="MARGHTPENPPAEGDVLVDLVGRLRTGSSLLAGTAARRMDQDLP
                     WYSSLSAEDRSWLGLVAQESIARFIAWLEDPRHSTTNSAEIFRVAPTELARAISLQQT
                     VGLIRLLVTVVEENTMELVSEAQQPVLREAVLTYSREVAFSAAEVYARAAESRGAWDA
                     RLEALAVDAIVRGAPDETLRSRVSTLGWTGEGRTLVMVGTSPTRLAERFTAELRQQAR
                     RLTSDALI"
BASE COUNT          480 a         1070 c         1011 g          445 t
ORIGIN      
        1 gcaacgccac ccaccagatg aagaagatga aggtcaagga cctgaaggcc ctgcgagacc
       61 tgctgcacat cccgatcacg gacgagcagc tcgaggccga cccgtacctc ccgccctact
      121 tcaagccgag cgacgacgac cccgcgctgc agtacatgct cgaacgccgc aagaccctcg
      181 gcggattcct gcccgagcgc aaggccccga agctgaacag cctcaccctc ccgggtgaca
      241 agccctacga gatcctcaag ggcggctccg gccagcagga ggtcgccacc accatggcgt
      301 tcgtccggct gctcaaggag ctcatcaagg acaaggagtt cggccggcgg atcgtgccga
      361 tcatcccgga cgaggcccgc accttcggcc tcgactcgat cttccccacg gcgaagatct
      421 tcaacacggc gggcatgaac tacaccgcgg tggaccacga cctgatgctc tcctacaagg
      481 agtccgaaca ggggcagatc ctgcacctcg gcatcaacga ggccgggtcg gccgccgcgc
      541 tccaggtggt cggcaccacg tacgccaccc agggcgagcc catggtgccg gtgtacatct
      601 tctactcgat gttcgggttc cagcggaccg gcgaccagtt ctggtccgcc ggcgaccagc
      661 tggcgcgcgg cttcgtcatc ggggcgacgg cggggcgcac cacgctcgcg ggcgagggca
      721 cccagcacat ggacggccac tcgcacatcc tggcggccac caaccacgcg ttcgtctgct
      781 acgacccggc gtacgcgtac gagatccgcc acatcgtccg cgacggcctg caacgcatgt
      841 acggccagga cgagagccgc gaccagaacg tcatgtacta cctcacggtg tacaacgagc
      901 ccatgcagca gccggccgag ccggatgacg tggacgtcga gggcatcctg aagggcatcc
      961 accgcgtggc ggaccacgac cacgcgggcg gctcgaaggt ccaactgctg gcctccggcg
     1021 tcggtctccc ctgggcgatg cacgccaagg agttgctccg cgacgactgg ggcgtggacg
     1081 ccgccgtctg gtccgtgacc tcgtggaacg agttgcgccg tgacggcctt gaggtcgaca
     1141 agcacaactt catccacccg gaggaggaga agcaggtggc tttcgtgacg cggaagctgg
     1201 cggagaccga cgggccggtc atctcgacgt cggactggga ccacatggtg caggaccaga
     1261 tccggccgtg ggttccccgc ccgtactaca cgctcggcgc ggatgggttc ggattctccg
     1321 acacccgcgc ggccgcccgc cggcacttcc tcatcgactc ccattcgatg gccacgcgcg
     1381 cgttgcaggc gctggtcgag gccgggcagt acgacggtgg cgcggtggcc cacgccatcg
     1441 cgaagtacga cctgttcaac gtcaatgccg gccggtccgg gagtgtgggc ggcgacgcct
     1501 gacccccgac gcctgactga gacgtggagt ggcccgcccc gaaaggggcg ggccactccg
     1561 ccttggcggg ccgcgggttc cagcgtgtca ggcttccagg gcctccacca actgctcgag
     1621 ttcggtcacc gcggggtcgt gggggtgggc gtcgccgaga cgggagacca gctcacgggc
     1681 cttgatctcg tccttcgcct ccacggcggc gaggaccagt tgccgcccgg tcgcgaactc
     1741 gtgtaccgct ggggtccact tcgcgacgcc gagtgacacg gcttggtggg ccaacccgtg
     1801 gtcgcggagc atctggaccg actgcgcgag ggctcgccca gtggtttcgc ggtcgcacgc
     1861 cgtcgccagg cggcgggcgg cgccgtcggg gtcgtcgacc accacgagcc gggccagctc
     1921 gatcagcggt gcccacgcat ggggattgcc ggcgtactcc tcggccagcg cccagagcgc
     1981 cgtgttgtgc tggtcggcca gcgtggcggg tgcctgcgtg gcggtcagcg ggtcgacgat
     2041 ctcgtcgggc acgggcgccg tgagtgcgtc ggcgaggtcc agcagggcct ctcgatcgtt
     2101 gggatcggcc tcgaggcgcg cgagcagcgc ggcctcctcc gcggcgtcgt tctgggcctg
     2161 tgcggattcg atctttgcct tgatccggtc aagaattccc atacccatga caataacgcc
     2221 tcccgccgcc ccggaggagc cgggtgggcg agaggctccg cgcaacgctg cccgacgcgt
     2281 ggcttcgtat gccatccaca aaaccccgcg ggccatatgc tcgctacatg gcccgaggac
     2341 acacaccgga gaacccgccc gccgagggtg atgttctggt cgatcttgtc ggaaggctac
     2401 gaaccggcag ttccctcctc gcgggcacgg ccgcccggcg catggaccag gacctcccct
     2461 ggtactcgtc actctccgcc gaggaccgtt cctggcttgg actggtcgcc caggagtcga
     2521 tcgcccggtt catcgcatgg ctcgaggacc ccaggcactc aaccaccaac tccgcggaga
     2581 tcttccgggt tgcccccacc gaactggcgc gcgccatctc gctgcagcag accgtcgggc
     2641 tcatccggct cctcgtcacc gtggtggagg agaacacgat ggagctggtc agcgaggcgc
     2701 agcaaccggt gctccgggag gcggtgctca cgtactcccg cgaggtcgca ttctcggcgg
     2761 cggaggtcta cgcgcgcgcc gccgagtcgc ggggggcgtg ggacgcccgg ctggaggccc
     2821 tcgccgtcga cgcgatcgtg cgcggcgccc cggacgaaac cctcaggtcc cgggtctcca
     2881 ccctcggctg gaccggtgag ggccgcacgc tcgtcatggt gggcacctcc cccacccggc
     2941 tcgccgagcg gttcaccgcg gagcttcgcc agcaggcccg ccggctgacg tcggacgccc
     3001 tcatcg
//
LOCUS       DASRKH010000341         2930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_59760, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000341 DASRKH010000000
VERSION     DASRKH010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59760"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VFC06_05430"
     CDS             complement(<1..655)
                     /locus_tag="VFC06_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZL81367.1"
                     /translation="MRAYELTGPILADEDRTVGKAWVVGGRMTYERPRHTDVTRVDGW
                     VIPGMVDMHCHLSVGPSGPVDGDLVVKQAMIERDAGVLMVRDTGSASSLDVLDGRRDV
                     PKVYRSGRFIARPKRYLKGFAVEVEPDELASEAARQAKASGSWVKIIADWIDRDLGDG
                     ADLAPLWSAQQWSDAVAAAHREGARVTAHTFATESLDALLDARLDCIEHGTGMTDVHM
                     "
     gene            complement(652..2244)
                     /gene="ffh"
                     /locus_tag="VFC06_05435"
     CDS             complement(652..2244)
                     /gene="ffh"
                     /locus_tag="VFC06_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HZL81368.1"
                     /translation="MFASLSDRLTATFRNLRGKGRLTEADIDDTVREIRRALLDADVA
                     LAVVTTFTDRIRARAVGVEVSGALNPGQQVVKIVHEELIEILGGSSRPLTIAKSGPTV
                     IMLAGLQGSGKTTFAGKLAHHLKSQGHTPLLVAADLQRPNAVTQLEVVGERAGVPVFA
                     PERGVTREDEKVRGNPVKVAGNAIKEAKRRQHSVVIVDTAGRLGIDQELMKEAGDIRR
                     AVDPDEVLFVIDSMIGQDAVATAQAFQAGVAMTGVVLTKLDGDARGGAALSVREVTGV
                     PIMFASTGEKLDEFEVFHPERMAGRILDMGDVLSLIEKAEATFDQAEAQRMADKMSKG
                     EDFTLTDFLSQMQQLKNMGSMKKMLTMLPGVGQMRDQIDNLDDRELTRTEAIIQSMTP
                     YERENPKVLNGSRRSRIAAGSGTSAAQINSLVKRFDDAQRMMKAMAGGGGMPGAGGLP
                     AMGPGGLPGGKKSKGKQAPQRKVKGKSGNPAKRAAQERALREQGPAAPASGSAFGLGQ
                     AKPEEPAFDPSQLPSLDKYLRG"
     gene            complement(2273..>2930)
                     /locus_tag="VFC06_05440"
     CDS             complement(2273..>2930)
                     /locus_tag="VFC06_05440"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZL81369.1"
                     /translation="SFMRCPDLESPWPPEALDRFTELLRNSHHLVSVWEAIDVAGQIV
                     RWLPQWAPVRNRPQRNAFHVYTVDRHQIETVVLAGKLRRQVADPDLLLCAALWHDIGK
                     QAGMGDHSVAGAALIPGIARHMGLPDATARDIETLVREHLTLADFATTRDAEDPAVAA
                     ELIERLGGRADLFEVLRALTEADAKAASPKAWTAWREQLIDTLTARVRATLARGPQVG
                     "
BASE COUNT          432 a         1001 c          969 g          528 t
ORIGIN      
        1 ccatgtggac gtccgtcatc cccgtgccgt gttcgatgca gtccaaccgg gcatccagga
       61 gagcgtccag tgactcggtg gcgaaggtgt gcgcggtcac cctcgcgccc tcgcggtgag
      121 cggccgccac ggcatccgac cactgctggg cggaccacaa cggcgccaag tccgcgccat
      181 cgccaaggtc ccgatcgatc cagtcggcga tgatcttgac ccaggatccc gaggccttcg
      241 cctgacgggc cgcctcggac gccaattcgt cgggttcaac ctcgacggcg aagcccttca
      301 ggtatcgctt gggacgggca atgaaacggc ccgagcggta caccttgggg acatcacgtc
      361 gtccgtccag cacgtcgagt gacgacgcgg agccggtgtc cctcaccatc agtactcccg
      421 cgtcgcgctc gatcatcgcc tgcttcacca caagatcgcc gtccaccgga ccggaggggc
      481 ccaccgacag gtggcaatgc atgtcgacca tgccgggaat gacccagcca tcgacgcgcg
      541 tgacgtcggt gtgtcggggc cgctcgtacg tcatgcggcc gcccaccacc caggccttgc
      601 cgactgtccg gtcctcgtca gcgaggatcg gcccggtcaa ctcgtaggcc ctcatccgcg
      661 caggtacttg tccaggctgg ggagctgcga cgggtcaaag gccggctcct cgggcttggc
      721 ctgccccaag ccaaacgcgc ttcccgatgc gggagcggcc gggccttgct cgcgcaacgc
      781 acgctcttgg gctgcgcgct tggccgggtt gcccgacttg cccttcacct tgcgttgcgg
      841 cgcctgcttt cccttggact tcttgccacc cgggagtccg cccggaccca tggctggcag
      901 gccgcccgca ccgggcattc ccccgccacc ggccatcgcc ttcatcattc gctgggcgtc
      961 gtcgaatcgc ttgacgagcg agttgatttg cgccgctgac gtgccggaac ccgccgcgat
     1021 gcgggaacga cgcgaaccgt tgagcacctt ggggttttcg cgctcgtagg gagtcatcga
     1081 ctggatgatg gcctcggtgc gcgtgagttc ccggtcgtcc aggttgtcga tctgatcgcg
     1141 catctgcccc acgccgggga gcatggtcag catcttcttc atcgagccca tgttcttgag
     1201 ctgctgcatt tggctcagga agtcggtgag agtgaagtcc tcacccttgg acatcttgtc
     1261 ggccatgcgc tgggcctcgg cctggtcgaa cgtggcctcc gccttctcga tgagcgacag
     1321 cacgtcgccc atgtcaagga tgcgcccggc catgcgttcc gggtggaaga cctcaaactc
     1381 gtcgagcttc tcccccgttg acgcgaacat gatggggacg ccggtcacct cgcggactga
     1441 gagcgccgcg cctccgcgcg catcgccatc cagcttggtg agcaccacac ccgtcatggc
     1501 gacgcctgcc tggaacgcct gcgcggtggc gaccgcgtct tgaccgatca tcgagtcgat
     1561 gacgaacagg acctcgtcgg gatcgacagc cctgcggatg tcgccggcct ccttcatcaa
     1621 ctcctggtcg atgccgagtc gaccagccgt gtcgacaatc accacggagt gctggcgcct
     1681 cttcgcctcc ttgatggcgt tgcccgccac cttcacgggg ttgccgcgca ccttctcgtc
     1741 ctcgcgcgtc acgccccgct ctggggcgaa gacgggcacg cccgcgcgtt cgcccaccac
     1801 ctcgagctgg gtgacggcgt tggggcgctg caggtcggcg gccacgagca gcggcgtgtg
     1861 gccctggctc ttcaggtggt gcgcaagctt gcccgcgaat gtggtcttgc cggagccctg
     1921 caggccggcg agcatgatga cggtgggtcc ggacttcgcg atggtgaggg ggcgagaact
     1981 gccgccgagg atctcgatga gttcctcgtg cacgatcttg acgacctgtt gcccggggtt
     2041 gagcgcgccg gagacctcaa ctcccacggc gcgggcacgg atccgatccg tgaaggtggt
     2101 gaccaccgca agggcgacgt cggcgtcgag cagggcgcgc ctgatctcgc ggacggtgtc
     2161 gtcgatatcg gcttctgtga gccttccctt gccgcgaagg ttgcggaaag tggctgtcag
     2221 tcggtcggac aggctggcga acacggctct cccttcggcg tggcggtgtc gactagccta
     2281 cctggggccc gcgggccagg gtcgcgcgca cgcgggccgt gagagtgtcg atgagctgtt
     2341 cgcgccacgc ggtccacgcc ttggggctgg ccgccttggc atcggcctcg gtgagggcgc
     2401 gaagcacctc aaacaggtcg gccctgccgc cgagtcgctc gatcagttcc gctgcgacag
     2461 cggggtcttc cgcatcgcgg gtggtggcaa agtcggcgag agtcaggtgt tcgcgcacca
     2521 gggtctcaat gtcacgcgcc gtggcgtccg gcaagcccat gtgccgcgcg atccctggaa
     2581 tcaaggccgc gcccgctacc gagtggtcgc ccatgcctgc ctgcttgccg atgtcgtgcc
     2641 acagggccgc gcagagcagc aggtcggggt cggccacttg cctgcgcagc ttccccgcga
     2701 gcacgacggt ttcgatctgg tggcggtcca ccgtgtacac gtgaaaggcg ttgcgctgcg
     2761 ggcggttgcg cacgggcgcc cactgcggta gccatctgac aatctgtcca gccacatcga
     2821 tcgcctccca cacgctcacc aggtggtgag agttgcggag cagttcggtg aatctgtcga
     2881 gcgcctcggg cggccacggg ctttccagat ccgggcaccg catgaatgac
//
LOCUS       DASRKH010000342         2404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_48458, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000342 DASRKH010000000
VERSION     DASRKH010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48458"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VFC06_05445"
     CDS             complement(<1..211)
                     /locus_tag="VFC06_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81370.1"
                     /translation="MTGFAWMGMGLFWLALLGLIVWLVIRLLPGKSQEPKTATSAAVS
                     PVASASAPALAILDERLANGEVDVET"
     gene            complement(348..1481)
                     /locus_tag="VFC06_05450"
     CDS             complement(348..1481)
                     /locus_tag="VFC06_05450"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZL81371.1"
                     /translation="MVLADRTDLATRLVLAIAAVVGVGVATAWAVGASIGPSLFHEHL
                     LRAVESGESPTDHAERAYATASAVSLSIALVTALIASAGVSVLVARRIRRSLAPFALA
                     AQRVADGERNVQVIPPGIGPEFDRVADAFTAMATELSHVEDTRTRMLADLAHEMRTPL
                     AVLAAYLEAIGDGVEHVDERTVQTMQDQVSRLTRLSADVALVTSAEEGRLSLARRPID
                     LDNVVRETAAQVSDRLGEHGITLDVTSVSGLLVNADADRIGQVLTNVLENARQHTLTG
                     GAVYVEVTGEPGVARVTVRDTGEGIAAEHLPRVFDRFFRVDAARDRAHGGSGIGLSIA
                     RAIANAHGGTLIATSDGAGTGATFSLTLPRLPSDDGLANVTED"
     gene            complement(1490..2227)
                     /locus_tag="VFC06_05455"
     CDS             complement(1490..2227)
                     /locus_tag="VFC06_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZL81372.1"
                     /translation="MTTPLASSEPLPRATILVVEDEESLGGLIASYLSRDGYDVEVVR
                     DGLEAIARARATPPDVVVLDLGLPGADGIEVCRTLRTFSDCYVIILTARVEEVDRLIG
                     LSVGADDYLTKPFSPRELVARVGVMLRRPRRFAGGAIAPETHDTLTVGDLSLDLSARE
                     VRVAGRLVELTRTEFDVLAALARQPRRAFSRDQLTDQVWGPAWFGDSHLVDVHVGHVR
                     RKLGDNPAAPVYVQTVRGYGYRLGPGQ"
BASE COUNT          427 a          786 c          785 g          406 t
ORIGIN      
        1 acgtctccac gtcgacctcg ccgtttgcca ggcgctcgtc aaggattgcc agcgcgggcg
       61 ctgaggccga tgccacgggc gagactgctg ccgaagtggc ggtcttcggt tcttgcgact
      121 tgccaggcaa cagtctgatc acgagccaca cgatgagccc aagaagggcc aaccaaaaca
      181 ggcccatgcc catccacgca aacccggtca tgcctccgcc ataccaaccc atcatggtcc
      241 tgtcctctcg tgtcgttgct ttccagcatc cacgagggag accctgattt tcgtaggcgt
      301 ttgacgaaga ttcgatgaag acggcatcgg gtacctgagg gtcgagatca gtcttcggtg
      361 acgttggcga ggccgtcgtc gctcggcaag cgcggcaggg tcaggctgaa ggtggcgcct
      421 gtgcccgcgc catcgctcgt ggcgatgagc gtgccgccgt gcgcgttggc gatcgcgcgg
      481 gcgatggaga gcccaatgcc ggatccgccg tgagcgcggt cgcgtgcggc gtcgacacgg
      541 aagaaccggt cgaatactcg ggggaggtgt tcagccgcga tgccctcgcc ggtgtcacgc
      601 acggtgaccc tggcgacgcc gggctcccct gtgacttcaa cgtacacagc gccacccgtg
      661 agtgtgtgct gtcgagcgtt ctcgagcaca ttggtaagca cctggccaat ccgatcggcg
      721 tcggcgttaa caaggagccc ggaaacgctg gtcacatcga gtgtgatgcc atgctcgccg
      781 agccggtccg agacctgggc ggctgtctcc ctcacgacgt tgtcgaggtc gatgggacgt
      841 cgggccaggg acagccggcc ctcctcagct gaggtcacga gggccacatc cgcggacagc
      901 cgagtgagtc ggctcacctg gtcctgcatc gtctgaactg tgcgctcgtc gacatgctcc
      961 acgccgtctc cgatcgcctc gaggtaggcg gcgagcacgg ctagcggtgt gcgcatctcg
     1021 tgggcaaggt ctgcgagcat tcgtgtgcgg gtgtcttcga cgtgcgacaa ctccgtcgcc
     1081 atcgccgtga acgcatcagc gacccgatcg aactcgggcc cgatacccgg cgggatcacc
     1141 tgcacgtttc gttcgccatc ggcgacccgt tgggcggcca gcgcgaacgg tgcgagtgag
     1201 cgtcgaatcc gtcgggcgac cagaacactc acaccagctg acgcgatgag tgccgttacc
     1261 agggcgattg atagggaaac ggccgacgct gtggcgtagg cgcgctccgc atggtccgtc
     1321 gggctttctc cgctctcgac tgcgcgcaga agatgctcat ggaacaggct tggaccgatg
     1381 gacgcgccga cagcccacgc ggtggcaaca cccacaccga cgacggccgc aatcgcgagt
     1441 accaggcggg tggccagatc ggtgcggtcc gccaggacca tccaccgtct cactggcccg
     1501 ggcccaatcg atagccgtaa cctctcacgg tttgcacata gaccggagcg gcggggttgt
     1561 cgccgagctt gcgacgcacg tggcccacgt gaacgtcgac gaggtgagag tcgccaaacc
     1621 atgcgggacc ccacacctgg tcggtgagct gatctcggga gaaggccctg cgtggttggc
     1681 gcgctaacgc ggctagcacg tcgaactccg tacgcgtgag ttccacgagt cgacccgcga
     1741 cgcggacctc tctggccgag agatcgaggc tgagatcgcc taccgtcagc gtgtcgtgcg
     1801 tttccggagc gatcgcacca ccggcaaagc gccgtgggcg ccgcaacatc acgccgactc
     1861 gcgccacaag ttcgcgaggg ctgaacggct tggtcaaata gtcgtcggcc ccgaccgaca
     1921 acccgatgag tctgtcaact tcttccaccc gggcagtaag gatgatcacg tagcagtcgc
     1981 tgaaggtccg caacgttcga cacacctcga tgccgtccgc gcctggcagc cccaggtcca
     2041 agacgacaac atccggggga gtggcccgtg cgcgagcgat cgcctcgagc ccgtcccgca
     2101 ccacctccac gtcgtatccg tcgcgtgaga ggtaggacgc gatcaatcca ccaagcgact
     2161 cctcgtcttc caccaccaag atcgtggcgc ggggcagcgg ctcggacgag gcgagtggcg
     2221 ttgtcatgtg cccatcctga cacttgcgtg aagacgcctc gtcgcaacgc ggtcgtagcg
     2281 ctacgtggct gtgacaggga tcatgaggac aggcccgggt gaggggcgtg tgtacaggta
     2341 gccctgggct cggctccagc cctgccggag taggactcgg tgctgggcct ccgtctccac
     2401 gccc
//
LOCUS       DASRKH010000343         2143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_29093, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000343 DASRKH010000000
VERSION     DASRKH010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29093"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            466..>2143
                     /locus_tag="VFC06_05460"
     CDS             466..>2143
                     /locus_tag="VFC06_05460"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HZL81373.1"
                     /translation="MEGPEIQFAEAVIDNGKYGTRTVRFETGRLAQQAAGTVAAYLDN
                     DTMLLSATTAGKHPREGFDFFPLTVDVEERSYAMGKIPGSFFRREGRPSTDAILTCRL
                     IDRPLRPTFVSGLRNEVQVVITVLSINPDDSYDTLAINAASASTQLSGLPFTGPVAGV
                     RIALVDGQWIAFPKYSQMERAVFNMVVAGRIAGDDVAIMMVEAEATNDSFNLISEGAQ
                     APTEAVVSEGLEAAKPFLKALCEAQIELAGKSPKEPKEFPRFLDYQDDVLAAVTAASE
                     ADLAAALRIADKHERENRLDEIKAAAIESLSGQFEGREKEISAAIRAVTKKTIRARIL
                     TDGIRIDGRGLKDIRPLSAEVASIPRVHGSAIFERGETQILGVTTLNMLKLEQQIDSF
                     NPEKKKRYMHHYNFPPFSTGETGRVGSPKRREIGHGALAERALVPVLPSREDFPYAIR
                     QVSEALGSNGSTSMGSVCASTLSMLNAGVPLRAPVAGIAMGLVSDTVDGETRYAALTD
                     ILGAEDAFGDMDFKVAGTSEFITAIQLDTKLDGLPASVLVGALAQAKDARL"
BASE COUNT          362 a          726 c          674 g          381 t
ORIGIN      
        1 cgagccctgt tccgcgccat tggcgcaatc cgtgccgccg aacgcaagta tcggccgcca
       61 agtggctcat tccgtaccgg gcacgcgcgt gcccggtacg gaatgagcca ccgggtggtt
      121 ggcgccgcgc gtgcccggta cggaatgagc caccgggtgg ttggcgccgc gcgtgcccgg
      181 tacggaatga gccacttggc ggccgatact tgcgttcggc ggcacggatt gcgccattgg
      241 cggggggagg gctcgccacg gggcccctct gctagtcttg gagcactcgc atgagcgagt
      301 tcaacctgca taccgagcgg gtcggccgga cgctggtcct cggtggagat gctcagaacc
      361 ccctctcttg gtagttctga gcgtgtcgac tgacgatcag ccggggctct cgaaagccca
      421 gtcctgcctg ctcgctcctt cattacaagg agcgtgagac cttaaatgga aggtcccgaa
      481 atccaatttg ccgaagccgt tatcgataac ggcaagtacg gcacccgcac cgtccgattc
      541 gagaccggcc gcctggccca gcaagctgct ggaaccgtgg ccgcgtacct cgacaacgac
      601 acaatgctgc tgtcggccac cacggccggc aagcacccgc gtgaaggctt cgacttcttc
      661 cccctcacgg tggacgtgga agagcgttcg tacgcgatgg gaaagattcc cggctcgttc
      721 ttccgtcgtg agggtcgccc ctcgaccgac gcgattctga cgtgccgtct gatcgaccgc
      781 cccttgcgcc ccaccttcgt gtcgggtctg cgcaacgagg tccaggtcgt catcaccgtc
      841 ctgtcgatca accccgacga ctcgtacgac accctggcca tcaacgccgc atcggcgtcg
      901 acccagctgt cgggcctgcc gttcaccggc cccgttgccg gtgtgcgcat cgccctggtc
      961 gacggccagt ggatcgcgtt ccccaagtac tcgcagatgg aacgcgccgt gttcaacatg
     1021 gtcgttgccg gacgcatcgc gggtgacgac gttgcgatca tgatggtcga agcggaagcc
     1081 accaacgact ccttcaacct gatctccgag ggtgcccagg ctcccaccga ggccgtggtt
     1141 tccgagggcc ttgaggccgc taagccgttc ctcaaggcgc tgtgcgaggc tcagatcgag
     1201 ctcgcaggca agtcgcccaa ggagcccaag gagttcccgc gtttcctgga ctaccaggac
     1261 gacgtgcttg ccgccgtcac cgcagcttcc gaggcggacc tggccgccgc gctgcggatt
     1321 gcggacaagc acgagcgcga gaaccgcctc gacgagatca aggcagccgc catcgaatcg
     1381 ctcagtgggc agttcgaggg tcgcgagaag gaaatctccg cggcgatccg tgcggtcacc
     1441 aagaagacga tccgcgcgcg catcctgacg gatggcattc gcatcgacgg ccgtggcctc
     1501 aaggacatcc gtccgctgtc ggccgaggtc gcctcgatcc cgcgcgtgca cggctcggcc
     1561 atcttcgagc gcggcgagac ccagatcctg ggtgtcacca cgctgaacat gctcaagctg
     1621 gagcagcaga tcgactcctt taacccggag aagaagaagc gctacatgca ccactacaac
     1681 ttcccgccgt tctcgacggg tgagacgggt cgtgtgggtt cccccaagcg tcgcgagatc
     1741 ggtcacggtg ctctcgctga gcgtgccttg gtgcccgtgc tcccgagccg cgaggacttc
     1801 ccctacgcca tccgtcaggt gtccgaggcg ctcggctcga acggctcgac ctcgatgggt
     1861 tccgtgtgtg cctcgaccct gtcgatgctc aacgcaggtg tgccgttgcg cgcacccgtt
     1921 gcgggcatcg ccatgggact cgtgtccgac accgttgacg gtgagacccg ctacgcggca
     1981 ctcaccgaca tcctgggtgc cgaggacgcg ttcggcgaca tggacttcaa ggtggccggt
     2041 acttccgagt tcatcaccgc gatccagctc gacaccaagc tcgacggcct cccggcctcg
     2101 gtgcttgtgg gcgcgcttgc gcaggccaag gacgctcgtc tgc
//
LOCUS       DASRKH010000344         4975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_25780, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000344 DASRKH010000000
VERSION     DASRKH010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 4975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4975
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25780"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..1975)
                     /gene="typA"
                     /locus_tag="VFC06_05465"
     CDS             complement(68..1975)
                     /gene="typA"
                     /locus_tag="VFC06_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HZL81374.1"
                     /translation="MPLRSDLRNVAIVAHVDHGKTTLVDAMLWQSGAFGEHEHVAERA
                     MDSGDLEREKGITILAKNTAVHYRGPAAKAVGEDGITINVIDTPGHADFGGEVERGLS
                     MVDGVVLLVDASEGPLPQTRFVLRKALVARLPVVLVVNKVDRPDSRISEVVQETTDLL
                     LSLASDLADEEEDLDLDSVLDLPVVYASARAGRASLTQPADGELPDKPNLEDLFTTII
                     ERIPPPEYDEAMPLQAHVTNLDASPFLGRLALLRIHNGTLRKGQLVGWARRDGSLRTV
                     KVTELLRTEALDRVPAEEAHAGDIVAVAGIDEITIGETLVDPEDPRPLPLITVDDPAI
                     AMTIGINTSPLAGRVKGSKVTARQVKDRLERELVGNVSLKVLPTERPDAWEVQGRGEL
                     ALAILVEQMRREGFELTVGKPQVVTRTIGGKIHEPIERMTVDVPEEHLGTVTQLMAAR
                     KGRMVTMTNHGTGWIRMEFLVPARGLIGFRTKFLTETRGTGIASSIAEGYEPWAGPIE
                     FRTTGSLVADRSGVATPYAMLNLQDRGSFFVEPTSEVYEGQVVGENTRSEDMDVNITR
                     EKKLTNIRSSTSDVFENIAPPRRLTLEESLEFAAEDECVEVTPIAVRIRKVVLEANER
                     FKIAARGRRSS"
     gene            complement(2073..3896)
                     /locus_tag="VFC06_05470"
     CDS             complement(2073..3896)
                     /locus_tag="VFC06_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZL81375.1"
                     /translation="MRRGDTILEVRGLNVDFFVEDQWYPAAIDVSYDVHAGEVLAIVG
                     ESGSGKTQSSMALLGLLPPNGRTSGSAKLMGNELVGMSTAKLRRVRGNDIAVIFQEPM
                     TALNPVYTVGFQIVEALRTHFDMSPEQAKARAIELLRMVEIPEPEVRFNSYPHQLSGG
                     QRQRAMIAQSLACDPRLLVADEPTTALDVTVQAEILKLMRDLRNRIDSGIILITHDMG
                     IVADMADTMVVMRNGRIVERGTSHQIFKEPQHPYTQELLAAVPHLGSHSGGSAEADTL
                     VPGAETGEAAMRDGAPTKAAARVTERPAPATAFTFDPANVLQAQDMVIEYPKHGRTPA
                     FRAVDGVTMSIKRGEVVGLVGESGSGKTTIGRAVVGLLPVIAGHLTIDGISMVGAKHK
                     DLKPLRAKVGIVFQDPGSSLNPRLPIGESIGEPLMLHSGIKGSELGKRVEQMLDQVEL
                     PRSFRNRYPHELSGGQRQRVGIARSLILEPSLLVADEPTSALDVSVQARVLDLFTDLQ
                     REHGFACLFISHDLAVVEMLSSRIAVMHNGKLVEYGDAGAIVNHPRHPYTQRLIAAVP
                     VPDPEEQKVRREARDRILAAHAAGRESDPGDEATMSRRSEQ"
     gene            3992..4558
                     /locus_tag="VFC06_05475"
     CDS             3992..4558
                     /locus_tag="VFC06_05475"
                     /inference="COORDINATES: protein motif:HMM:NF022216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HZL81376.1"
                     /translation="MRPTVVYRTAVSTVLARVSVVLAVLAIAYFAVTGGVPEVIRSGG
                     VAGLFATSMWVSFALPHVTVSDGGVTVRNVLRTTHVPWPIFTGVDSRWALTIHTTEGD
                     VGSWAIPAASGFAARSSRDVAGQAERGVNANTVALAIGARHRELRDAGYLGRRTVQRV
                     LTERTWNRGAITAVAVALAVAATWALLG"
     gene            complement(4561..>4975)
                     /locus_tag="VFC06_05480"
     CDS             complement(4561..>4975)
                     /locus_tag="VFC06_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019309499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZL81377.1"
                     /translation="LREREFIDAARVAGASHARIIFKHMLPNAIGVIIVNVTLLMSAA
                     ILLETALAYLGFGITLPNISLGQMINEYQGAFNTRPWLFWFPGMFIISIALSVNFIGD
                     GLRDAFDPRQRRIPSVRKMRRAEMRAQRAAANTAS"
BASE COUNT          694 a         1671 c         1783 g          827 t
ORIGIN      
        1 gacgtgcggg cgcccgcgcc ggggctgccg gcgccggcct ggggctcgac gtcgtccggg
       61 gcgggcatca gctcgaccgg cggccccgcg ccgcgatctt gaaacgctcg ttcgcctcca
      121 gcaccacctt gcggatgcgg acggcgatgg gggtgacctc cacgcactcg tcctcggcgg
      181 cgaactcgag cgactcctcg agtgtgagcc tgcgcggggg cgcgatgttc tcgaacacgt
      241 cggacgtgga tgagcggatg ttcgtgagct tcttctcgcg ggtgatgttc acgtccatgt
      301 cctcggagcg ggtgttctcc cccaccacct ggccctcgta gacctccgag gtgggctcga
      361 cgaagaacga cccgcggtcc tgcaggttca acatcgcgta cggggtggcg acaccggagc
      421 ggtccgccac cagcgagccc gtggtccgga actcgatcgg cccggcccac ggctcgtatc
      481 cctcggcgat cgaggaggcg atgccggtgc cgcgggtctc ggtgaggaac ttggtccgga
      541 acccgatgag gccacgagcg ggcaccagga actccatgcg gatccagccg gtgccgtgat
      601 tggtcatggt gaccatgcgg cccttgcggg cggccatcag ttgggtcacg gtgcccaggt
      661 gctcctccgg gacgtcgacc gtcatgcgct cgatgggctc gtggatcttc ccaccgatgg
      721 tccgggtgac cacctggggc ttccccaccg tcagttcgaa gccctcgcgg cgcatctgct
      781 ccacgaggat ggcgagggcg agttcaccac gcccctgcac ctcccacgcg tcgggccgct
      841 cggtgggcag caccttgagg gacacgttgc cgacgagttc cctctcgagc ctgtccttga
      901 cctgccgcgc ggtgaccttg ctgcccttga cccgtccggc gagcggtgag gtgttgatcc
      961 cgatggtcat ggcgatcgcg gggtcgtcca ccgtgatgag cggcaacggg cgcgggtcct
     1021 ccgggtccac cagcgtctcc ccgatggtga tctcgtcgat ccctgcgacg gcgacgatgt
     1081 cacccgcgtg ggcctcctcg gccgggacac ggtcgagcgc ctcagtcctg aggagctcgg
     1141 tcaccttcac ggtgcgcagt gacccgtcgc ggcgcgccca cccgaccagt tggcccttgc
     1201 gcagcgtgcc gttgtggatc cgcaggagcg cgaggcggcc gaggaacggg gaggcgtcca
     1261 ggttggtgac gtgtgcctgc agcggcatgg cctcgtcgta ctcgggcgga gggatgcgct
     1321 cgatgatcgt ggtgaagagg tcctccagat tcggcttgtc cggcagttcg ccgtcggccg
     1381 gctgggtgag ggacgcccgg cccgcccggg cggaggcgta caccaccggc aggtcgagga
     1441 cggagtcgag gtccaggtcc tcctcctcgt ccgcgaggtc ggaggcgagg gagagcagca
     1501 ggtcggtggt ctcctggacc acctcactga tgcgggagtc cggccggtcc accttgttca
     1561 cgacgagcac gacgggcaac cgcgccacga gcgccttgcg cagcacgaag cgcgtctggg
     1621 gcagcggccc ctcggacgcg tccacgagca gcacgacgcc gtcgaccatg gagagcccgc
     1681 gctccacctc gccgccgaag tcggcgtggc cgggggtgtc gatgacgttg atcgtgatcc
     1741 cgtcctcgcc caccgccttc gcggccggcc cacggtagtg gaccgccgtg ttcttggcga
     1801 ggatcgtgat gcccttctcg cgctcgaggt caccggagtc catggcacgt tccgcgacgt
     1861 gctcgtgttc accgaaggcc ccggactgcc acagcatggc atcgaccagg gtggtcttcc
     1921 cgtggtcaac gtgtgccacg atcgcaacgt tgcgcaggtc ggagcgcagt ggcatacgga
     1981 ctcttttcgt catgaggggt gcccggtgag tctacccgcg gtggcacccg caccacgagg
     2041 tgtgccccca tgagggtggt cacacggcgg agctactgtt cggacctcct gctcatcgtg
     2101 gcctcgtcgc cggggtccga ctcccggccg gccgcatggg cggcgaggat gcggtcccgc
     2161 gcctcgcggc gcaccttctg ctcctcgggg tccggcaccg gcacggcggc gatgagccgc
     2221 tgggtgtacg ggtgacgcgg gtggttgacg atcgccccgg cgtccccgta ctcgacgagc
     2281 ttgccgttgt gcatgacggc gatgcgcgag gacagcatct cgaccacggc gaggtcgtgc
     2341 gagatgaaca ggcacgcgaa gccgtgctcg cgctgcaggt ccgtgaacag gtcgagcacg
     2401 cgggcctgga ccgacacgtc cagggcggac gtcggttcgt ccgccacgag cagcgacggc
     2461 tcgaggatga gcgaacgggc gatgcccacg cgctggcgct gcccacccga cagttcgtgc
     2521 gggtagcggt tgcggaacga gcgcggcaac tccacctggt ccagcatctg ctccacgcgc
     2581 ttgcccagtt cggagccctt gatgcccgag tggagcatca gcggttcgcc gatggactcg
     2641 ccgatcggga gccgcgggtt cagcgaggag cccgggtcct ggaacacgat tcccaccttg
     2701 gcccgcaggg gcttcaggtc cttgtgcttc gcgcccacca tgctgatgcc gtcgatcgtg
     2761 aggtggcccg cgatgacggg caaaaggccg acgacggcgc gcccgatagt cgtcttgccc
     2821 gagccggact cgcccacgag tcccacgacc tcgccccgct tgatcgacat cgtgacgccg
     2881 tcgacggcgc ggaacgcggg ggtgcgcccg tgcttgggat actcgatcac catgtcctgg
     2941 gcctggagca cgttcgcggg atcgaaggtg aaggcggtgg cgggcgcggg ccgctcggtg
     3001 accctggcgg cggccttggt gggggcgccg tcgcgcatcg cggcttcacc ggtctcggca
     3061 cccgggacca gcgtgtccgc ctccgcggaa ccgccggagt gcgagccgag gtgcggcacc
     3121 gcggccagca gttcctgggt gtaggggtgc tgcggctcct tgaagatctg gtgcgacgtg
     3181 ccgcgctcca cgatgcggcc gttgcgcatc accaccatgg tgtcggccat gtccgcgacg
     3241 atgcccatgt catgggtgat gaggatgatc ccggagtcga tgcggttgcg caggtcacgc
     3301 atcaacttga ggatctcggc ctggacggtc acgtcgaggg cggtggtggg ctcgtccgcc
     3361 acgaggagcc gcgggtcgca ggcgagcgac tgggcgatca tggcgcgctg ccgctgcccg
     3421 ccggagagct ggtgggggta ggagttgaac cgcacctccg gctcggggat ctccaccatg
     3481 cgcaacagtt cgatggcgcg ggccttcgcc tgttccggcg acatgtcgaa gtgggtgcgc
     3541 agcgcctcca cgatctggaa accgaccgtg tagaccgggt tcagcgcggt catgggctcc
     3601 tggaagatga cggcgatgtc attgccgcgc acgcggcgca gcttcgccgt gctcatgccg
     3661 accagttcgt tccccatgag cttcgccgaa ccggacgttc gcccgtttgg cgggagcagg
     3721 ccgagcagcg ccatggagga ctgggtcttg ccggagccgg actcgccgac gatcgcgagg
     3781 acctccccgg cgtgcacgtc gtaggacacg tcgatcgccg cggggtacca ctggtcctcc
     3841 acgaagaagt cgacgttgag gcccctcacc tcgaggatcg tgtcccccct gcgcatgagg
     3901 tcgccctcac cgggcagcgc gggtgcggtt tgcgtcacgc ggatcgttgt ccttccgtgt
     3961 gttcgggtgc gaatgctgcg aggatggtgc ggtgcgtccc accgtcgtct accgaacggc
     4021 cgtctccacg gtgctggcac gtgtctcggt ggtcctcgcg gtgctcgcga ttgcctactt
     4081 cgccgtcacc ggcggtgtgc ccgaggtgat tcgctcgggc ggagtcgcgg ggctgttcgc
     4141 cacctcgatg tgggtgtcgt tcgccctccc ccacgtgacc gtgtccgacg gcggcgtgac
     4201 ggtgcgcaac gtcctccgga ccacccacgt gccgtggccg atcttcaccg gcgtcgactc
     4261 ccggtgggcg ctgacgatcc acaccacgga gggcgacgtc gggtcgtggg ccattcccgc
     4321 ggcctccggg ttcgccgccc gctcgtcgcg cgacgtcgcc ggtcaggccg agcggggcgt
     4381 caacgcgaac accgtggcac tggcgatcgg cgcgcgccac cgggagctgc gcgacgccgg
     4441 ctacctgggt cggcgcaccg tccagcgtgt gctgaccgag cgcacctgga accgcggcgc
     4501 gatcacggcg gtcgccgtgg cgctggccgt ggctgccacc tgggcgctcc tgggatgaca
     4561 tcacgaggcc gtgttcgccg ccgcgcgctg cgcccgcatc tccgcgcgcc tcatcttgcg
     4621 cactgagggg atccgccgct ggcgcggatc gaaggcgtcg cgcaggccgt cgccgatgaa
     4681 gttgacggac aaggcgatgg agatgatgaa catgcccggg aaccagaaga gccagggccg
     4741 ggtgttgaac gccccctggt actcattgat catctggccc agcgagatgt tcggcagcgt
     4801 gatgccgaag ccgaggtagg cgagcgccgt ctccagcagg atcgccgcgc tcatgagcag
     4861 ggtgacgttg acgatgatca cgccgatcgc gttgggcagc atgtgcttga agatgatccg
     4921 cgcgtgggac gcacccgcca cacgggcggc gtcgatgaac tcgcgctccc tgagg
//
LOCUS       DASRKH010000345         3391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_10960, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000345 DASRKH010000000
VERSION     DASRKH010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10960"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /locus_tag="VFC06_05485"
     CDS             complement(<1..1098)
                     /locus_tag="VFC06_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HZL81378.1"
                     /translation="MTPVQLAGLVVLAIVALFVISAIARSIMVVRQTQAVLVERLGRY
                     SRTMEAGLHLMVPFIDRVRAKVDLREMVYSFPPQSVITSDNLVVDIDSVIYFQVTEPK
                     SAVYEIASYIQGVEQLTTTTLRNIVGTMDLEQTLTSRDQINGQLRGVLDEATGKWGIR
                     VNRVELKAIEPPASIKDSMEKQMRAERDRRAAILTAEGVKQSAILTAEGAKQSAILTA
                     EGEAQGRILRAEGESRAILQVFDAIHEGDADSKLLAYQYLQMLPEIAKNDSNKVWFVP
                     TEFTGALKAVSADVNLVVRRLPRSGAAVSGSGSSTPPKAALTALSAPVNSVGTNHTLF
                     ESFLAISGSIWRYWYASSLESASPSWMASNTC"
     gene            complement(1095..1541)
                     /locus_tag="VFC06_05490"
     CDS             complement(1095..1541)
                     /locus_tag="VFC06_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HZL81379.1"
                     /translation="MDSIWWFVGAAALGAVEIFTLDLTFLMLASGAIAGGVVVLVGGP
                     IWLAFVVALVVATAMLAALRPWLLRSMRVRGVDLVETNVAAIVGTKGRTIDPITETGG
                     RIKLRGEVWTARIQDDAATIDEGVEVIVTKIVGATAIVVPEKEHTA"
     gene            complement(1691..2476)
                     /locus_tag="VFC06_05495"
     CDS             complement(1691..2476)
                     /locus_tag="VFC06_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018134048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZL81380.1"
                     /translation="MSEVLRLDDVTLRRGENQILDGITWGVDPTDRWVILGPNGAGKT
                     SLITVCSARAHPTTGSARILGEQLGETNTHELRIRVGLTSAALADRIPPHETVHDIVM
                     TAAYGVTGRWREEYEQVDEARAEALLSAFGMHAFADRTFETLSEGERKRVQIARALMT
                     DPELLLLDEPAAGLDLGGREELLSALTELAGDWRSPAMVMVTHHVEEIPAGFTHALLM
                     RDGRIVAQGRLVDVLTSANLSQTYGLPIQLFAQDGRWTARQSS"
     gene            complement(2488..>3391)
                     /gene="glgA"
                     /locus_tag="VFC06_05500"
     CDS             complement(2488..>3391)
                     /gene="glgA"
                     /locus_tag="VFC06_05500"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HZL81381.1"
                     /translation="HGIPHVLSAHSLEPLRPWKAEQLGGGYRVSSWIEKTAYEAADGI
                     IAVSAGMRADVLRCYPDVDPAKVHVVHNGIDVDAWAAPATDEERAAADAVVARHGIDP
                     ARPAIVFVGRITRQKGLPYFLKAVEQLPQEIQVVLCAGAPDTAQIAQEVSGAVAKLQK
                     ERDGIIWIEEMLPRAELVAVLDACTTFVCPSVYEPLGIVNLEAMAVGLPVVATATGGI
                     PEVVEHGVTGMLVPIDQVQDGTGTPTNPEKYSADLAVALTAMVSDLDAAKAMGKAGRQ
                     RAADHFAWGAIAERTLEVYSHVLG"
BASE COUNT          603 a         1118 c         1071 g          599 t
ORIGIN      
        1 gcaggtgttc gatgccatcc acgagggcga cgccgactcc aagctgctgg cgtaccagta
       61 cctccagatg ctccccgaga tcgccaagaa cgactcgaac aaggtgtggt tcgtccccac
      121 cgagttcacg ggcgcactca aggcagtcag cgccgccttt ggcggggtgg aagagccgga
      181 gccgctcacg gccgcgccgg atcgcggcaa gcgccgcacc accaagttga cgtcggcgct
      241 gactgccttg agtgcgcccg tgaactcggt ggggacgaac cacaccttgt tcgagtcgtt
      301 cttggcgatc tccggaagca tctggaggta ctggtatgcc agcagcttgg agtcggcgtc
      361 gccctcatgg atggcgtcga acacctgcaa gatggctcgc gactcgccct cggcacgcag
      421 aatgcggcct tgagcctcgc cctccgccgt gagaatcgcg gactgcttgg caccctcggc
      481 cgtgaggatc gcggactgct tcacaccttc ggccgtgagg atcgcggcgc gacggtcacg
      541 ctcggcacgc atctgcttct ccatcgagtc cttgatcgat gcgggcggct cgatggcctt
      601 gagctccacg cggttgacgc ggatgcccca cttgccggtc gcctcgtcca gcactccgcg
      661 cagctgaccg ttgatctggt cacgcgacgt cagcgtctgc tcgaggtcca tggtgccgac
      721 gatgttacgc agcgtcgtgg tggtgagctg ctccacacct tggatgtagc tggcgatctc
      781 gtagaccgcc gacttgggct ccgtcacctg gaagtagatg accgagtcga tgtcgacgac
      841 caggttgtcc gacgtgatga cggactgcgg ggggaaggag tagaccatct cgcgaaggtc
      901 aaccttggcc cgaacgcggt cgatgaacgg caccatcagg tgcagcccag cttccatagt
      961 gcggctatag cgaccgagac gctcgaccaa gacggcctgc gtctggcgga ccaccatgat
     1021 cgaacgcgcg atcgcggaga tcacgaagag ggcgacaatg gcgaggacga caagtccagc
     1081 cagctgtacg ggagtcatgc tgtgtgctcc ttttctggaa cgacgatggc cgtcgctccg
     1141 acaatcttgg tgacgatgac ttcgacgccc tcgtcaatag tggctgcatc atcttggatg
     1201 cgcgccgtcc acacttcgcc gcgcagtttg atgcggccgc cggtttcggt aatggggtcg
     1261 atcgttcggc ccttggtccc cacgatcgcg gccacattgg tctccaccaa gtcgacgccg
     1321 cgtacgcgca tcgaacgaag caaccaaggc ctgagcgccg ccagcatcgc ggtcgcgaca
     1381 acaagggcaa ccacgaatgc gagccagatc ggcccgccga ccaagaccac gacacctcca
     1441 gcgatcgcgc ctgacgcgag catgaggaac gtgagatcga gggtgaagat ttcgacggct
     1501 cctaacgcgg cggcaccgac aaaccaccaa atcgaatcca tggcgactcc tttcagtttg
     1561 gacgctacca ggcctgaagc cacgcggaat cggccgccag atggacgccg tcgtcgatcg
     1621 tcatccctgg ggatgacgcg acactctcag ccatcctcgt ttcgcgaatt ccccccaggt
     1681 aaaccgtgcg ctaggacgat tgacgtgccg tccagcggcc gtcttgagca aacagttgaa
     1741 tgggcagtcc gtacgtttgt gaaaggtttg cgctcgtcag aacgtccacg agtcggccct
     1801 gggcgacgat tctgccgtcg cgcatcagga gtgcgtgggt gaacccggcg gggatctcct
     1861 caacgtggtg cgtcaccatc accatggcgg gcgagcgcca gtcgcccgct agttcggtga
     1921 gcgcagataa cagctcctcg cggccaccca gatcgaggcc agctgctggc tcgtcgagga
     1981 gcagaagttc ggggtccgtc atgagcgcgc gcgcgatctg gacgcgcttg cgctcgcctt
     2041 cgctcaacgt ctcaaacgtc cgatccgcga aggcatgcat cccgaaggcg gataacagag
     2101 cctcggctcg cgcctcgtct acctgttcgt attcttcgcg ccaccgcccg gtcacgccgt
     2161 acgcggccgt catcacaatg tcgtgcaccg tctcgtgtgg cgggatgcgg tcggcaagcg
     2221 cggccgaggt gaggcccacg cggatgcgca actcgtgggt gttggtctcg cccagttgct
     2281 cgccaagaat tcgggccgag ccggtggtgg gatgtgcgcg tgcggaacac accgtgatga
     2341 gggaggtctt gcccgcgcca ttggggccaa gaatcaccca gcgatccgtc ggatcgacgc
     2401 cccacgtgat gccgtcgagt atctgattct cgccacggcg caaggtgaca tcgtcaagcc
     2461 gtaacacttc gctcatattg gtcgacccta gcccagcacg tgcgagtaga cctcgagcgt
     2521 gcgttccgcg atggcacccc acgcgaagtg gtcggccgca cgttgccgcc cagccttgcc
     2581 catcgccttt gcggcgtcga ggtcggacac catggcggtc agcgccacag cgaggtcggc
     2641 cgaatacttc tcggggtttg tcggcgttcc ggtgccgtcc tgcacctggt caatgggcac
     2701 gagcattccc gtcactccgt gctccaccac ctcgggaatc cctcccgtag cggtcgcgac
     2761 gacgggcagt cccacggcca tggcctcaag attgacgatg ccgaggggct cgtacacgga
     2821 gggacacacg aacgtggtgc aagcatcgag caccgcgacg agttcggcgc gcggcaacat
     2881 ctcctcgatc cagatgatgc cgtcgcgctc cttctgaagc ttcgcgaccg cacccgagac
     2941 ctcttgagcg atctgcgcgg tgtcgggagc ccctgcgcac aacacgacct gaatctcctg
     3001 aggcagctgc tcgaccgcct tcaggaagta cggcaggccc ttctggcggg tgatgcggcc
     3061 aacaaacacg atcgcggggc gagcggggtc gatgccgtgc cgggcgacca cggcgtcggc
     3121 cgctgcccgc tcttcgtcgg tggccggcgc ggcccacgcg tcgacgtcga tgccgttatg
     3181 caccacgtgc accttggcag ggtcgacgtc cgggtagcag cgcaacacgt ccgccctcat
     3241 gcccgcggac acggcgatga tgccgtccgc cgcttcgtac gccgtcttct cgatccaact
     3301 tgagacgcgg tatccgccgc ccaactgctc ggctttccac ggccgcaagg gctcgaggga
     3361 atgcgcgctc agcacgtgcg ggatgccgtg c
//
LOCUS       DASRKH010000346         2972 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_24407, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000346 DASRKH010000000
VERSION     DASRKH010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24407"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..309
                     /locus_tag="VFC06_05505"
     CDS             112..309
                     /locus_tag="VFC06_05505"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HZL81382.1"
                     /translation="MGYLGILGSRAKVARLLRGRGDPADLPPHVRAPMGLPIGSHTPA
                     EIAVSVAAELVAVRGTSPDRW"
     gene            complement(964..1308)
                     /locus_tag="VFC06_05510"
     CDS             complement(964..1308)
                     /locus_tag="VFC06_05510"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZL81383.1"
                     /translation="MATRTPEEVFGHHGQSLGAEDLEEIVADYADDAILIVQGKTYHG
                     KAGAREVFIQLLSDVPHAAWDLNQVFDDDVLYLEWKATGGGRKVEDGIDTFIFKDGMI
                     RVQTVVYHVQPA"
     gene            1467..2507
                     /locus_tag="VFC06_05515"
     CDS             1467..2507
                     /locus_tag="VFC06_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HZL81384.1"
                     /translation="MRAVRLRRYEVRPVVEEVPEPQVTHPFDVIVRIGGAGLCRTDLH
                     IVEGQWAPKTHVPLPYTLGHENAGWIHAVGSAVEHLHPGDAVILHPLVTCGFCRACRA
                     GDDVHCESQRFPGIDTDGGMAEFILTTARSVVKLPAGVEPAAVAALADAGLTAYHAAK
                     KAVPLLFPGSTCVILGAGGLGHLGIQCLRAMSATRIIVLDPNEEALALTRGWGADETV
                     VVDGKHVDRVLEMTDGMGAEVVLDFVGEQGAERDSWAMTRRAGSDFVIGYGGTVDIPT
                     IDVISTERNVVGNLVGSYNDLVELMALNAAGHVTMLTHTYPLDAVNEAMDDLDSGRLR
                     GRGILVPASAVT"
BASE COUNT          486 a         1027 c          998 g          461 t
ORIGIN      
        1 cccgaggtag cccaccgggt ggtcccacag cgcgtccagc gcgacggcgt cgctcgccgc
       61 cccagcggtc atcacggcca cgtaggtccg ctcccctgct gggaccaccc ggtgggctac
      121 ctcgggatcc tcgggagtcg cgcgaaggtg gcccggctgc tgcgcggacg cggcgacccg
      181 gcggatctac caccgcacgt ccgcgcgccg atgggactgc cgatcgggag ccacacgccg
      241 gcggagatcg cggtgagcgt ggccgcggaa ctggtggccg tccgcggcac ctcccccgat
      301 cgctggtaag agttcgtgcc cacggtcagg ggcatacccc acaccctcgc ggcaaacact
      361 caccatccgc cccggaccaa gattcgcttc acgcgcttcc gcgcgatggc aaccgataca
      421 gtccgcgtgt tgccagggtc ggtgtatcgc atgtcggtgc atggcggtgc atttcatggc
      481 tgccggcctt cgatttcatg gccgctggcc ctggaatggc accgccaagc gatacagaac
      541 tgcgggactg tatcgcaaac ccgcgtgagg ccgacgacgg cgaccacccc gagcgggatg
      601 agaccgccgg ctaggaccag ttgcccgtgg cgagaccacc gatgacctgg atcaacatcg
      661 ggatcaacaa caatcccccg gccaggccgg cgatgacggg gatgaggccg actccctgcc
      721 caacttgtat gcccggatcc cgaacagcac ggtgcaaacg gttgagaccg cgtagaggac
      781 gccgctcaca atggccaggg tcaggcctgc catgttcagc ggttcgcccc caactgcctc
      841 catttaggcc ccctcgccga agtaaaaaac tccgatgagc gtggcgaaca tgagtaccag
      901 ggatacccag gcccggttca ggttcgtgcg tgcgcgttgg gactgcgctt gcgtaccggg
      961 ccgtcaggcg ggctgcacgt ggtagacgac ggtctggacc cggatcatcc cgtccttgaa
     1021 gatgaaggtg tcgatgccgt cctcgacctt tcggccgccg ccggtggcct tccactcgag
     1081 gtagaggacg tcgtcgtcga agacctggtt gaggtcccac gccgcgtgcg ggacgtcgct
     1141 gaggagctgg atgaagacct cgcgggcccc ggccttgccg tggtaggtct tgccctggac
     1201 gatgagaatc gcgtcgtcgg cgtagtccgc gacgatctcc tcgaggtcct ccgcgccgag
     1261 ggactggccg tggtgtccga agacctcttc cggggtgcgc gtcgccattg aaatcgcctt
     1321 ccgttctgtc gattgccggc cgggaatcgg cttcccggcc agcctgagtc tcctccaagc
     1381 ctccaccgct gtccataccc ggggcggccc gtcctgagta gagttccaag cgggatccca
     1441 ttctcaacgg tgcgttcgga ggcgtcatga gggcagtgcg gctacgcagg tacgaggtcc
     1501 gtccggtcgt cgaggaggtg cccgaaccgc aggtcaccca cccgttcgac gtcatcgtgc
     1561 ggatcggcgg tgcgggcctc tgccgcaccg acctgcacat cgtcgagggc cagtgggcgc
     1621 cgaagaccca cgtgccgctg ccctacacgc tcggccacga gaacgcgggc tggatccacg
     1681 ccgtcggctc ggcggtggag cacctccacc cgggcgacgc cgtcatcctc cacccgctcg
     1741 tgacgtgcgg gttctgccgg gcgtgccgcg ccggcgacga cgtccactgc gagtcccagc
     1801 ggttccccgg catcgacacc gacggcggga tggcggagtt catcctgacc accgcccggt
     1861 ccgtggtcaa gctgccggcc ggggtcgagc ccgcggcggt cgcggcgctg gccgatgcgg
     1921 gcctgacggc gtaccacgcc gcgaagaagg ccgtgccgct gctgttcccg gggagcacct
     1981 gcgtgatcct gggcgccggt ggcctcgggc acctcgggat ccagtgcctg cgggcgatga
     2041 gcgccacccg catcatcgtg ctcgatccca acgaggaggc ccttgccctc acccgcggct
     2101 ggggagcgga cgagaccgtc gtcgtggacg gcaagcacgt cgaccgggtc ctcgagatga
     2161 ccgacggtat gggcgccgag gtggtgctgg acttcgtcgg ggagcagggc gccgagcgag
     2221 acagttgggc gatgaccagg cgggccggga gcgacttcgt gatcgggtac ggcgggactg
     2281 tcgacatccc gaccatcgac gtgatctcga ccgagcgcaa cgtcgtcggg aacctcgtag
     2341 gcagctacaa cgacctcgtc gagctcatgg cgctcaacgc cgccggacat gtcacgatgc
     2401 tcacccatac ctacccgctg gacgcggtga acgaagcgat ggacgacctc gactccggtc
     2461 ggctgcgcgg ccgcggcatc ctcgtccccg cgtccgccgt gacgtagccg ccaagaatgg
     2521 gcggaggcaa cagggccgtg gacgagcaaa acctgctctc ggcggtggcg ccctccgagc
     2581 cggggctcgc ggccaactac tgaaagacgg cgcccgcgtc ggaagcacga cgaccgggca
     2641 cggacaaacc ggagttcgca tccgcactgt ggcttccgga cgccgggtcg acgaactgga
     2701 acgccgggcg tggcgcccaa tcccgggctt caagcgggcg gatcgactcg aatccgtccg
     2761 gagaccccat cattcaggca ttctcgttcg acccgttctt gcctgcagtg tccctctctc
     2821 ccgccgattc gcgattcgcg cccgaccggg tcctctgccg attctcggcc cagttcgatc
     2881 tgacgagccc gcggcggtga aacggcgcat ccaaggatca gcaacaggac aaaagcgaca
     2941 ccggtggcac tgaggatgta ccacggccag gg
//
LOCUS       DASRKH010000347         2205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_10971, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000347 DASRKH010000000
VERSION     DASRKH010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10971"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..103
                     /locus_tag="VFC06_05520"
     tRNA            32..103
                     /locus_tag="VFC06_05520"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:64..66,aa:Gln,seq:ttg)
     gene            167..1732
                     /gene="glmU"
                     /locus_tag="VFC06_05525"
     CDS             167..1732
                     /gene="glmU"
                     /locus_tag="VFC06_05525"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006590533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HZL81385.1"
                     /translation="MSVTRPAAVMILAAGEGTRMKSSTPKVLHRIGGRSLLHHAITAA
                     QGLDPERLVVVVRHAREQVAAHAHEVAPDALMADQDDVPGTGRAVWCGLSALDATAMA
                     GAAARGLAREDVITVQATGPIVVTSADVPLVDTELLDAMLADHVAGGRAVTVMTARVD
                     DATGYGRIVHDADGAIASIVEHKDATAAERAITEVNAGLYVFDAATLRGALAGLDRDN
                     AQGELYLTDVVAIARAAGTLVGAFVAPDAQAVEGVNDRIQLSALGAVLNRRVVNRWMR
                     DGVTIVDPATTWIDVTATLEPDSTLLPGTQLHGHTSVATGAVVGPDSTLTDVTVGAGA
                     TVIRTHGSLSTIGPGATVGPFSYLRPGTVLGADGKIGAFVETKNATIGAHSKVPHLSY
                     VGDATIGEYSNVGAATIFVNYDGVHKNHTTVGDHVRIGSDNSLIAPLTIGDGAYTGAG
                     AIIRRDVPAGALARDSVPQQIIEGWVASRRPGTPSAEAAAKAAGRDQDGARVSREPIS
                     FSQDDTANERGEQ"
     gene            1729..>2205
                     /gene="prs"
                     /locus_tag="VFC06_05530"
     CDS             1729..>2205
                     /gene="prs"
                     /locus_tag="VFC06_05530"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HZL81386.1"
                     /translation="MTSLISKSNERRLVFTSGRAHPELAEHVAEELGIEAVPTTAYTF
                     ANGELYVRFGESVRGADAFVLQSHAAPINEAIMEQLIMVDALKRASAQRITVIAPSYG
                     YARQDKKHKGREPISARLMADLFKTAGADRIMSVDLHTSQIQGFFDGPVDHLWAMPL"
BASE COUNT          382 a          746 c          730 g          347 t
ORIGIN      
        1 ctggtgcgcc gggactcaat ttccatcccg atccgcccta gtgtaatggc agcatgcagg
       61 cctttggagc cttgcggtcc aggttcgaat cctgggggcg gaactcgcgc cgttagactg
      121 accccgtcag ccctacgggg atcccggtcc cacacaaggg ggtgcggtga gcgtcacacg
      181 accagccgcc gtcatgatcc tcgcagcggg cgagggcacc cgcatgaagt cttcgacgcc
      241 caaggtgctg catcgcatcg gtggacggtc attgcttcat cacgcgatca cagcagccca
      301 gggcctcgac ccagaacggc ttgtggtggt cgtgcggcac gcccgcgagc aggtggcggc
      361 gcacgcgcat gaggtcgcgc ccgatgccct gatggccgac caggacgacg ttcccggcac
      421 cggacgcgcc gtctggtgcg ggttgtccgc gctcgacgca acggccatgg ccggtgctgc
      481 ggcgaggggc cttgcccgcg aagatgtcat cacggtgcag gccactgggc ccattgtcgt
      541 gacctccgca gacgtgccac tcgtggacac ggagttgctc gacgccatgc tcgcggacca
      601 cgtggccggc ggccgcgcgg tcactgtcat gacggcccgc gtggacgacg ctacgggtta
      661 cgggcgcatc gttcacgatg cggacggcgc cattgcgagc atcgtcgaac acaaagacgc
      721 gaccgccgcc gagcgcgcca tcaccgaggt caacgcaggg ttgtacgtct ttgacgccgc
      781 cacgctccga ggcgcgctgg ccggacttga tcgcgacaat gcccaaggcg agctgtatct
      841 gaccgacgtc gtcgcgatcg ccagggccgc gggcacgttg gtaggtgcgt tcgtggcgcc
      901 ggacgcccag gccgttgagg gcgtcaacga ccgcatccag ctgtccgccc tgggagcagt
      961 cctcaaccgg cgggtggtga accggtggat gcgcgatgga gtcaccatcg tcgacccggc
     1021 caccacctgg atcgacgtga ccgccacgtt ggaaccggat tcgaccctgc tgcccggaac
     1081 ccagttgcac ggccacacgt ctgtcgccac cggcgcggtc gtgggacccg actccacgct
     1141 gaccgacgtc acggtcggcg cgggtgcgac cgtcatccgc acgcacggct cactgtcgac
     1201 gatcggtccg ggagccaccg tggggccgtt ctcctatctg cgccccggca cggtactcgg
     1261 agcggacggc aagatcggcg cgttcgtgga gaccaagaac gcgaccatcg gcgcgcactc
     1321 caaggtgccg cacctcagct acgtgggcga cgcgaccatc ggcgaatatt cgaatgtggg
     1381 tgccgccacc atcttcgtga actacgacgg cgtccacaag aaccacacga cagtgggcga
     1441 ccacgtgcgc atcggctccg acaactcact catcgcaccg ctcacaatcg gcgacggtgc
     1501 gtataccggc gcgggcgcga tcatccgccg cgacgtcccc gcaggtgccc ttgcgcgcga
     1561 cagcgtgccg cagcagatca tcgagggctg ggttgcgtcc cggaggccag ggacgccatc
     1621 tgcggaggcg gccgcgaagg cggcgggccg ggaccaggac ggcgcgcgcg tgtcgcgaga
     1681 accgatatcg ttcagtcagg acgacacagc taacgagcgg ggtgagcaat gacgtctttg
     1741 atctcgaaat cgaacgagcg caggttggtc tttacgagcg gtcgggcgca ccccgagttg
     1801 gcggagcatg ttgccgagga actcggcatc gaagccgtcc ccaccaccgc atacacgttt
     1861 gcaaacggcg agttgtacgt ccgctttggc gaatcggtgc gcggcgccga tgcctttgtg
     1921 ctccaatcgc acgcggcccc catcaacgaa gccatcatgg agcagctcat catggtggac
     1981 gcgctgaagc gagcgtcggc ccagcgcatc accgtgattg cgccgtccta cggctacgcc
     2041 cgtcaggaca agaagcacaa gggtcgcgag ccgatctcgg cgcgactgat ggccgatctc
     2101 ttcaagaccg ccggtgcgga ccgcatcatg tccgtcgacc tgcacacgtc gcagatccag
     2161 ggcttctttg acggccccgt tgaccacctg tgggccatgc cgttg
//
LOCUS       DASRKH010000348         3023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_15324, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000348 DASRKH010000000
VERSION     DASRKH010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15324"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VFC06_05535"
     CDS             <1..239
                     /locus_tag="VFC06_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81387.1"
                     /translation="SVSVESHGFVARHRGFATRLAAVRHFRDYLLLDRGDSLHLDAGN
                     ERALDDYFEIRGTKGAGPVDVNALTVALTLHPNI"
     gene            282..1472
                     /locus_tag="VFC06_05540"
     CDS             282..1472
                     /locus_tag="VFC06_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81388.1"
                     /translation="MNDCSAPIRGHEPDSQQERDCPVHGTKRSTHPMDAARLMAPPTA
                     VLSHGRERMELNVEMEFGGAYAIGKDGTIEHTGDYVPSFIDDRCDSEDWEPMHGFTNQ
                     YGYKGPVMHASEQLGGGMEDWVWEHPGTYAIVEATYTVDEDGNELGMGAFDDPPEGWV
                     LLRHRDADDSAPKPQSTVPQWYGEFSPIGKPITPGAVIWAQVDGGERYFRLLPFEPDG
                     SGNPRGQERLWVSNDDENVVRRNSELGDWMPSFFPHTEAGDVEHAYRNLFNDIVRTDK
                     RLEGQRNDVDNQLTEAGVPGVSLAEARGFDNDKPFSAVYGANSDGTRSFRVVVARDAD
                     GAAQVEASGDTSSRRAKSAIGVMRANADRIADWRDAREAKSAQHKQADELRDRYLRAT
                     GERL"
     gene            1574..2665
                     /locus_tag="VFC06_05545"
     CDS             1574..2665
                     /locus_tag="VFC06_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81389.1"
                     /translation="MAICSAPKRGHEPNSDESRKCPVHGVAGGLLAGGSKGSGAPTPP
                     QGDVVRPGEDFVVSVKGFWVNQNELPPRARKTRRVEHPYSTTVSVRSVASEDAPVSMV
                     VDEPIGGPKEYRHDGNGWFVRHLPYAHQTEPSIAGGPHFRREHEMPRYDCCEYDSEDE
                     FEEGAWAYADQFLIVDGVVWQRAPEPTYLIATFGLGGNHGGTGLMVGAPRSPNEHSNT
                     YSANQFDLAKQRAIEVAAARGDTESVKRFETMTEPLIHVDDPSLITFVREPSEEEIAE
                     SPLSDPEELDKASHSTAWVVRDNVAKHPNTAPAVLVRLANDSSEDVRDRVARNPHTPT
                     SLLDIMADFDESQSVRTTAAKARATRQPE"
     gene            2693..>3023
                     /locus_tag="VFC06_05550"
     CDS             2693..>3023
                     /locus_tag="VFC06_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81390.1"
                     /translation="MTTGAIPCVNPTMCPTKSKTHTSFAELGKCAAMYGGDRGTASAP
                     ALPPTPVAGREKGAPSLEAQRAGTVLAELGPKEQGSAAEYLEASTSGAATIVLAACPD
                     ATAIEWAE"
BASE COUNT          582 a          983 c         1012 g          446 t
ORIGIN      
        1 catctgtctc ggtcgagagc catggctttg tcgcccgaca tcgtgggttc gccacccggc
       61 tggccgcggt gcggcacttt cgcgactacc tcctgttgga ccgtggcgac tccctgcacc
      121 tggacgccgg caacgagcgc gctcttgacg actacttcga gattcgcggc accaagggcg
      181 cgggaccggt agacgtcaac gcgctgaccg tggcgctcac gctgcacccc aacatctagg
      241 cgttcgatgc cgggtggata ccttccatcc aggaggtcct catgaatgac tgttccgcac
      301 cgatccgcgg ccacgaaccg gactcacagc aagaacgcga ctgcccggtc cacggcacga
      361 agcgctcgac gcacccgatg gatgcggcac gcctcatggc gccgcccacc gcggtgctca
      421 gtcacgggcg agagcgcatg gagttgaacg tcgagatgga gttcggcggc gcctacgcaa
      481 tcggcaagga cggcacgatc gagcacacag gcgactacgt cccctccttc atcgatgacc
      541 ggtgcgacag cgaggactgg gaacccatgc acgggttcac gaaccagtac ggctacaagg
      601 gcccggtcat gcacgcgtcc gagcaactcg gaggtggtat ggaggactgg gtgtgggagc
      661 accccggcac ctacgcgatc gttgaggcga catacacggt agacgaggac ggtaacgaac
      721 tcggcatggg tgccttcgac gacccgcctg aggggtgggt gctgctgcgc caccgcgacg
      781 cggacgactc cgcgccgaag cctcagtcga ccgtgccgca atggtacgga gagttctctc
      841 cgatcgggaa gcccattacg ccgggagcgg tcatttgggc ccaggtcgac ggcggtgagc
      901 ggtacttccg actgctgccc ttcgagccgg acggttcggg caaccctcgc ggacaggagc
      961 gcctgtgggt gtcgaacgat gacgagaacg tggtgcggcg caacagcgaa ctaggggact
     1021 ggatgccctc gttcttccct cacaccgaag cgggagacgt cgagcacgcg taccgcaacc
     1081 tcttcaacga catcgtccgc acggacaagc gcctcgaagg tcagcgcaac gacgtcgaca
     1141 atcaactcac ggaggccggt gtgccgggcg tcagcctggc ggaggcccgc gggttcgaca
     1201 acgacaagcc gttctcggct gtctatggag cgaactctga cggcacgcgc tcgttccggg
     1261 tagtggtcgc acgtgacgct gacggcgccg cacaggtgga ggcgtccggc gacacgagtt
     1321 cgcgccgggc caagtcagcg atcggcgtga tgagggccaa cgcggaccgc attgccgatt
     1381 ggcgtgacgc ccgtgaggcg aagtctgcgc agcacaagca ggcggatgaa ctgcgagacc
     1441 gctacctgcg cgccaccggc gaacggctct aacaggccct cgaacagcac tcgcgggggc
     1501 gattcgcttc tgcgtggccg accgcacgaa cggtgcgagg gcgggtggat accttctgcg
     1561 taggaggttc accatggcaa tctgttccgc cccaaagagg ggccacgagc ccaactcgga
     1621 cgagagtcgc aagtgtcccg tccacggtgt cgcaggcggc cttctcgccg gcggctccaa
     1681 gggctctggc gcaccgacac ccccgcaggg ggacgtcgta cgtccgggcg aggactttgt
     1741 ggtgagcgtc aagggtttct gggtgaacca gaacgaactc ccgccacgag cgcgcaagac
     1801 gcgccgcgtg gagcacccgt actcgacgac agtgtctgtg cgcagcgtcg cctccgagga
     1861 cgcccctgtg tccatggtcg tggacgagcc gatcggcggg cccaaggagt accgccacga
     1921 cggcaacggg tggtttgtgc ggcacctgcc atacgcgcac cagacggagc cctcgatcgc
     1981 cggcggccca cattttcgcc gggagcacga gatgcctcgc tacgactgct gtgagtacga
     2041 ctccgaggac gagttcgaag agggcgcgtg ggcgtatgcg gaccagttcc tgatcgttga
     2101 cggagtggtg tggcaaaggg cgccggagcc gacctacctg atcgctacct tcgggctggg
     2161 cggtaaccac ggcggcaccg ggctcatggt gggcgcgcct agaagcccga acgagcacag
     2221 caacacctac tcggccaacc agttcgacct cgccaagcag cgggcgatcg aagtggcggc
     2281 cgcgagaggt gacacggagt ctgtgaagcg tttcgagacc atgacggagc ccctcatcca
     2341 cgtcgacgac ccgagcctca tcacgttcgt tcgtgagccg tccgaggagg agattgccga
     2401 gtcgccgctg tctgaccccg aagaactcga caaggcgtcg cactcgacgg cgtgggtcgt
     2461 gcgggacaac gtcgcgaagc accccaacac ggcaccggcg gtgctcgtgc gtctcgcgaa
     2521 cgacagcagc gaggacgtgc gcgaccgtgt cgcacgcaac cctcacacac ccacaagcct
     2581 gctcgacatc atggctgatt tcgacgaatc gcagtctgtg cgtacgacag ccgccaaggc
     2641 acgcgcaacc cgtcaacccg agtaaccacc acccgtcaac caggagaccc acatgacaac
     2701 cggagcaatc ccttgcgtga acccgaccat gtgcccaacg aagtccaaga cgcacacgtc
     2761 tttcgctgaa ctgggcaagt gcgctgcaat gtacggcggg gaccggggca cggcaagcgc
     2821 ccccgcgttg ccgccaaccc cagtggccgg acgagagaag ggcgcaccaa gtctcgaagc
     2881 gcagcgggcg ggcaccgttc ttgcggaact cgggccgaag gagcaggggt cggcggcaga
     2941 gtacttggag gcttcgacca gcggcgcggc gacgattgtg ttggcggcct gccctgacgc
     3001 gacggcgatc gagtgggctg agg
//
LOCUS       DASRKH010000349         2938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_35688, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000349 DASRKH010000000
VERSION     DASRKH010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35688"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VFC06_05555"
     CDS             <1..507
                     /locus_tag="VFC06_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81391.1"
                     /translation="TLPAPAGDIQTGITRSVLLALELPQVPVGAVEIGTVTCTWTAVQ
                     GDLKETTRTIQVQALGSPDAAAVEEHVDQEVLCMAQLQLVADAHRGAVDAARRADEAE
                     YTAHMAKARGVLSELEEAGEPSAGPLLRMHEQIDADGVGGVLADRGLQLRTHRAQYRT
                     RRSRPVEE"
     gene            1122..1892
                     /locus_tag="VFC06_05560"
     CDS             1122..1892
                     /locus_tag="VFC06_05560"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HZL81392.1"
                     /translation="MGENSSTVGGLGFEDKLWATADKLRGNMDAAEYKHVVLGLIFLK
                     YISDAFMEKFTALEADELADPEDKDEYLAENIFWVPPVARWSFLQKNAKRPDIGTLID
                     DAMTAIERSNDTLKGVLPKDYARPALDKTRLGEIIDLIATIGLGDEKSRQHDILGRVY
                     EYFLARFAAAEGKLGGQFYTPQCVVKLLVEMLEPYRGRVFDPCCGSGGMFVQAAKFVQ
                     AHASGNGNGRKAKAGSAGFVLANGSLTSNTSCEGEIRR"
     gene            1919..>2938
                     /locus_tag="VFC06_05565"
     CDS             1919..>2938
                     /locus_tag="VFC06_05565"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZL81393.1"
                     /translation="MTTPYHSQYWAHALTLRGSGGDIAALSRSIANARVDLNPHQIDA
                     ALFALRSPFTNGAVLADEVGLGKTVEAGIVLSQRWAERRRRILIVVPAMLRKQWQQEL
                     SEKFFLPSEVLDSRVFNRLKKDGVANPFDLKDKAVICSYNFAAAKTAEVSQVPWDAVV
                     IDEAHRLRNVHRSRTRLQNNPAARKTMAHRITDAVGQAPKMLLTATPLQNSLLELFSL
                     VSVIDPYVFGDAASFREQFVNNPDEVSRNLQLKQRIASVCTRALRKQVTEYIPFTNRV
                     PLTQEFLPTDEEQALYDEITTYLQREVLYALPASQRQLITMVLRKLLASSTFAIARTL
                     RRLADR"
BASE COUNT          575 a          894 c          918 g          551 t
ORIGIN      
        1 acactaccgg cgccggcggg tgatatccag acgggtatca cccgctctgt cctcctggcg
       61 ttggagttgc ctcaggtacc ggtgggtgcg gtggagatag gcaccgtgac ctgcacgtgg
      121 acggccgtcc agggcgatct caaagagact acgcgcacca tccaggtgca ggccctgggc
      181 agcccggacg cggcggcagt cgaggagcac gtcgaccagg aagtcctgtg catggctcag
      241 ctgcagctgg tggccgacgc tcatcgcggg gcggtcgatg ctgctcggcg tgcagatgag
      301 gcagagtaca ctgcccacat ggccaaagcg cgcggggttc tctccgagtt ggaggaggcg
      361 ggggagccgt ccgcgggtcc gcttctgcgt atgcacgaac aaatcgatgc cgatggcgtg
      421 gggggtgtcc tcgccgatcg aggtctacag ctgcgaaccc accgggccca gtacaggacc
      481 cggcgctcca ggccggtcga ggagtagctt cgagagcgct cggggagcgg aagcggacgg
      541 cttaccccag gtccggggtc cgtttcgccc aggggcggcg acccagggtt accccgcaga
      601 cttctatagc tcccgctcgc ttcagggcgt gcgcgacttc gaacatcgtg tgaccatcgg
      661 tagaagcgtc atcgaacacg ggggtgcgct gaccccggat cagtccgggg tcagcaaggc
      721 ggagggcggc tcggactctc ttgcttctcc cccgcattcg ggtcccgctt tcagtctgtc
      781 cgacctctgt tgtataatgg cgaagcagag cacggatatc cacaccacgg caccgggtcg
      841 tcacgtggtg tggatctcgg gataggtaag tgtctgggcg ctggaggggg gtgtgctgcg
      901 gggtccgcgg ggaagggact tgcggttagg gacagcgtcc gcgcacggta gttatgtgat
      961 gtccgttcac ctgggtgatt gtgccaaagg tgcgcattca gaccgcgatt tcaagtaaca
     1021 gtccaagcta cttttgaatg aggtttgcga agtaggctgg tagtcgcgac caagagaccc
     1081 actacccaag cggggcagct atctcggaca aggttgggag tatgggcgag aacagcagca
     1141 ctgtgggtgg cctcggtttc gaggacaaac tctgggccac agccgacaag ctgcgcggca
     1201 acatggatgc cgccgaatac aagcacgtcg tcctcgggct gatctttctc aagtacatct
     1261 ccgatgcctt catggagaag ttcacggcgc ttgaagccga cgagctggcc gaccctgagg
     1321 acaaagacga ataccttgcc gagaacatct tctgggtacc gcccgtggcg cggtggtctt
     1381 tcctccagaa gaacgcaaag cgtcccgaca tcggcacttt gatcgacgac gcgatgacag
     1441 ccattgagcg gagcaacgat acgctcaaag gtgtgctccc caaagattat gcccgcccgg
     1501 ccctcgacaa gacccgactc ggcgagatca tcgacctgat cgccaccatc ggcctgggcg
     1561 acgaaaagag ccgacaacac gacatcctcg gccgtgtgta cgaatacttc ctcgcccgct
     1621 tcgctgcggc ggaaggtaag ctcggcggac agttctatac gccgcagtgc gtggtgaagc
     1681 tacttgtcga aatgctggaa ccctatcggg gccgtgtctt cgatccctgc tgcggctccg
     1741 gcggtatgtt tgtccaagcc gccaagttcg tacaggccca cgcctccggc aatggtaacg
     1801 ggaggaaggc caaggccggc tcggccgggt ttgtcctggc caacggctcg ctcaccagca
     1861 acacctcgtg cgagggtgag attcgccgct agttactaat gtcggggcaa gcaacgacat
     1921 gacgacgcca taccacagcc agtactgggc acacgccttg actcttcgcg ggtcaggcgg
     1981 ggacattgct gcgctgtcta ggtccatcgc caacgctcgg gtggacctga atccccatca
     2041 gatcgatgct gcgctcttcg cccttcgctc gccgtttacc aacggtgccg tcctggcgga
     2101 tgaggtgggg ctgggcaaga ccgtcgaagc tgggatcgtg ttatcccaac gctgggcgga
     2161 acgccgtcgg cgcatcctaa tcgtcgtgcc cgccatgctg cgcaaacagt ggcagcagga
     2221 gctcagtgag aagttcttcc tcccttcgga agtcttggat tcgcgcgtct tcaaccggct
     2281 caagaaggac ggggtggcga acccgtttga cctgaaagac aaggctgtca tctgctcata
     2341 caacttcgcg gctgccaaga ctgccgaagt atcgcaggtt ccatgggatg ccgtggtcat
     2401 cgacgaggcg catcggctcc ggaacgtgca tcgttcccgc acccgattgc agaacaaccc
     2461 ggcagccaga aagacgatgg cccaccggat tactgacgcg gttggtcaag cccccaagat
     2521 gctgttgacg gccacgcctc tgcagaactc actgctcgag ctcttcagtc tggtgagcgt
     2581 catcgatccg tacgtcttcg gtgacgccgc atcgttccgt gaacaattcg tcaataaccc
     2641 tgacgaggtc agtcgaaact tgcagctgaa gcagcggatt gcctctgtct gcacccgcgc
     2701 gctgcgaaag caggtaacgg agtacatccc gttcaccaac cgtgttcccc tcacgcagga
     2761 gtttctgccc accgacgaag aacaggccct ctacgacgag atcacgacat accttcagcg
     2821 cgaggtgctc tacgcccttc ccgcaagcca gcgacagctc atcacgatgg tcttgcggaa
     2881 gctgctcgca tcgtcgacgt tcgcaatcgc ccgcacgctt cgtcgacttg cagatcgg
//
LOCUS       DASRKH010000350         2435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_34806, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000350 DASRKH010000000
VERSION     DASRKH010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34806"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..282)
                     /locus_tag="VFC06_05570"
                     /pseudo
     CDS             complement(1..282)
                     /locus_tag="VFC06_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017946571.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4193 domain-containing protein"
     gene            complement(388..1212)
                     /locus_tag="VFC06_05575"
     CDS             complement(388..1212)
                     /locus_tag="VFC06_05575"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HZL81394.1"
                     /translation="MHSSIELMDLAATLAREAGELVIRGRARAAVTSTKTSPIDIVTQ
                     MDVAAETLLRSRLAEMRPDDAILGEEGEETAGASGVTWVVDPIDGTVNYLYGLPHFAV
                     SVAAVEGGTDPATWRILAGAVYGGDGVEWKAAAGHGAWRNGEPLVRSGGPTLSATLLG
                     TGFNYIADRRAAQGKIISQVLPQVRDIRRLGSAAVDLCLVAEGKLDAYYEHGLHPWDF
                     AAGALIASEAGVKVAGIDGGAADSHLLIAAVESVWDELRDVLDEAGARRPWDSPGA"
     gene            1285..2286
                     /gene="sepH"
                     /locus_tag="VFC06_05580"
     CDS             1285..2286
                     /gene="sepH"
                     /locus_tag="VFC06_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein SepH"
                     /protein_id="HZL81395.1"
                     /translation="MTGMSLKGLSEDGTHLIVVTTDGDEHRLAISDELRELVRHARVM
                     RRDEDAPATRLSPREIQQRIRSGLNAAELAELTGEPVDAIAKFEPPVLAEREYIVQLA
                     QTTRIGRDTTAPTLGDLVGDRLAGRGIDPESVLWDAWREIDEPWKVAVDYRVDGHTAR
                     ALWTFDHVAKALTAEDDESTWLTETELLDAPVPRRHLSAVRADSGSVPLHPHRPASPT
                     ATESAVVDNEARRDTTDALLEDLASRRGTREPLEIEDDEDDDGFEGFGPTRGAAPGRP
                     TTMPHPAGSARPASSGPEDQKPPSTPPTPPAGTDKPPRKGRASVPSWDEIVFGAKND"
BASE COUNT          441 a          842 c          754 g          398 t
ORIGIN      
        1 tgcagatcgg cttgtctccc ctgaattctg ccaactggct gcggtggtgc accaggaagc
       61 aggacatgca cgtgaactcg tcctgctgcg gcgggaggac cctgacggac agctcctcac
      121 ctgacaaatc ggcgccaggc aattcgaagc tgtcggctgc gtcagcctcg tcctcgtcga
      181 ccacgcccga actcttatcc gagcggcgtg ccttcaactc ttcgatagag tcagcggcaa
      241 tttcgtcgtc ggtcttgcgg gggcgtcgta gtcggtcgcc atccggtgtt ctccttcacc
      301 ttgtaggtgt ctgatgaatg cttgcggccg aggttttgtt cccactcagc gcaggcattc
      361 tgcaccacaa atggcattta cgcgagttca agctcccggc gagtcccaag gtcgacgtgc
      421 gccagcctcg tccagcacgt cgcgaagctc gtcccacacg ctctcgacgg cggcaatcag
      481 caagtgagag tcggccgctc cgccatcgat tcccgcgacc ttcactccgg cctccgacgc
      541 gatcaacgcc cccgccgcga agtcccaagg atggagcccg tgctcgtagt aggcatccag
      601 tttgccttcg gcgacgaggc agagatcgac ggctgctgag ccgagccgcc tgatgtcacg
      661 cacttgagga agcacctggg aaatgatctt gccttgggct gctctccgat cggcgatgta
      721 gttgaaaccg gtaccgagaa gcgtcgccga gagcgtcggg ccgccggaac gcaccagcgg
      781 ctctccgttt cgccacgcgc catgcccggc ggccgccttc cactcaacgc cgtcgccgcc
      841 gtacaccgct ccggcgagga tccgccacgt cgccgggtcc gtcccgccct cgacggcggc
      901 cacggaaacg gcaaagtgcg gcaggccgta gaggtaattg acggtgccat cgatggggtc
      961 aaccacccag gtcaccccgg acgctccagc cgtctcctcg ccctcctcac ccagaatcgc
     1021 gtcgtcgggt cgcatctcgg ccagccgtga tcgcagcaac gtctcagcgg ccacgtccat
     1081 ctgggtgacg atgtcgatcg gtgaggtctt cgtggaggtc actgcggcgc gtgccctgcc
     1141 ccgtatcacc agttctcccg cctccctggc aagagtggcg gcaaggtcca tgagttcgat
     1201 cgatgagtgc atgcgaaaag cgtcgcacac gacgcgcggg cgcggcacca cgcgcggcgg
     1261 cacccggcgt ggcacagtag agcaatgacg ggaatgagcc tcaagggact gtccgaagac
     1321 ggtactcacc tcatcgtcgt cacgacagac ggcgatgagc accgactcgc catttcggac
     1381 gagttgcgtg agttagtgcg ccacgcccgc gtgatgcggc gcgacgagga tgcccccgcc
     1441 acgcgactga gcccgcggga gatccagcaa cgcatccgct ccgggctcaa cgccgcggaa
     1501 ttggccgaac tcaccggcga accagtcgat gcgattgcca agtttgagcc ccccgtgttg
     1561 gcggagcgcg agtacatcgt gcaactggca caaaccacgc gcatcggtcg cgacacgacg
     1621 gcgcccacct tgggagacct ggtgggcgat cgcctcgccg ggcgcggaat tgaccccgaa
     1681 tccgtgctgt gggatgcgtg gcgagagatc gacgagccgt ggaaagtcgc cgtggactac
     1741 cgggtcgacg gccacaccgc ccgtgccctg tggaccttcg accacgtggc gaaagcgctc
     1801 actgccgaag acgacgagtc cacgtggctc accgaaaccg aacttctgga cgccccggtc
     1861 ccccggcgcc acctcagcgc agtgcgtgcc gattctggat cggtcccttt gcatccgcac
     1921 cggcccgcca gccccaccgc aactgagtcc gccgtcgtcg acaatgaggc gcgtcgcgac
     1981 accaccgacg ctctcctcga ggacctggcg agccgcaggg gcacacgcga acctcttgag
     2041 atcgaggacg atgaggacga cgacgggttc gagggcttcg ggcccacgcg tggcgccgcg
     2101 cccggtcgtc ccacgaccat gccccacccc gccggaagcg cccgcccggc atcgtcaggg
     2161 cccgaagatc aaaaacctcc gagtacgccg ccaacacctc cggcagggac cgacaagccg
     2221 ccgcgcaagg gaagggcatc cgtgcccagc tgggacgaga tcgtctttgg ggccaagaac
     2281 gactgaccgc gacgcggtct aggtctagag agacagtcgc gccgttcgtt gtggtgccgt
     2341 cagtttccct cggcaagaag caacggcacc tgcagttctt ctggcgtgag agacccgtgc
     2401 atcccgatca gcgacatcga ttggctgctc tggat
//
LOCUS       DASRKH010000351         2531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_28607, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000351 DASRKH010000000
VERSION     DASRKH010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28607"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="VFC06_05585"
     CDS             complement(<1..683)
                     /locus_tag="VFC06_05585"
                     /inference="COORDINATES: protein motif:HMM:NF013067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HZL81396.1"
                     /translation="MAVNYIGMALVLNCGSSSVKYQVVDTTVDEPLAVGLVERVTDHG
                     IAISEIIARLGDSNSGVDLAQLTVMGHRVVHGGAEFSAPTIIDDDVERRIALLSTLAP
                     LHNPANLAGISAARKAFPQLPHVAVFDTAFHQTMSEAAYTYAIDKDIAAEHAVRRYGF
                     HGTSYAYLAHEAPRVMGKRPDEVNIIALHLGNGASACAMRAGRAIDTSMGLTPLAGLV
                     MGTRTGDIDP"
     gene            complement(688..>2531)
                     /gene="pta"
                     /locus_tag="VFC06_05590"
     CDS             complement(688..>2531)
                     /gene="pta"
                     /locus_tag="VFC06_05590"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HZL81397.1"
                     /translation="AALSRIVTRYHEVARKCDVVVIVGTDYTDVAGPAEFELNAKVAA
                     NLGAPVALVVRGAGRSPSEIAEVLSQARSTMDENHAKVVAAFANRCDPDDVDAIKAAL
                     PEDIGVAALPEEPLLYAPTLGALRDAIDGKMIAGDEALLSREVRAITIAAMAVEHLLD
                     RLEEGALIIAPGDRADTLLAALTAHSAAGFPALAGIVVNGGFLPPPSVQRLIDGLGPT
                     LPIIATDLGTFETAHRCWNTRGRLSRESVLKVDMALNMFEHYVDGTQLTDRLNLAATD
                     VVTPLMFEYALLDRARTKRQRIVLPEGEDDRILRAAGTLLTRNVVDLTILGDPAAITS
                     RAGELGIDLSAAKLLSPSDPEHVDRFAAAYYEMRKHKGMTQEAARDRVQDVSYFGTMM
                     VELGLADGMVSGAAHTTAHTIVPSFQIIKTLPGVSVVSSVFLMCLADRVLVYGDCAVN
                     PDPTAEQLADIAISSAETAKQFNIEPRVAMLSYSTGESGTGSDVDKVREATRLVRERR
                     PDLLVDGPIQYDAAVEPSVARSKAPDSPVAGRATVLIFPDLNTGNNTYKAVQRSAGAV
                     AVGPVLQGLRKPVNDLSRGALVQDIVNTVAITAIQAQQLTADNAAEE"
BASE COUNT          396 a          779 c          905 g          451 t
ORIGIN      
        1 gggtcgatgt cgcccgtcct cgtgcccatg acgaggccag cgagcggggt gaggcccatc
       61 gaggtgtcga tcgcgcgccc ggcgcgcatc gcgcaggcgg aggcgccgtt gcccaggtgg
      121 agggcgatga tgttgacctc gtcggggcgc ttgcccatca cacggggcgc ctcatgcgcc
      181 agataggcat agctggttcc gtggaatccg taccggcgga cggcgtgctc ggcagcaata
      241 tccttgtcga tcgcgtaggt gtaggccgcc tccgacattg tctggtggaa agcggtgtcg
      301 aacaccgcga cgtggggtag ctgggggaat gccttacgtg ccgcactgat gcccgcgagg
      361 tttgccgggt tgtgcaacgg cgcgagcgtc gacagcaacg cgatgcggcg ctccacatcg
      421 tcgtcaataa tcgtgggcgc ggagaactcg gcgccgccgt gcactacccg gtgccccatc
      481 acggtcaatt gggccaggtc aacacctgag ttcgagtcgc caagcctcgc gatgatctcc
      541 gagatcgcta tgccatgatc ggtgacgcgt tcgacgaggc cgacggccaa gggttcgtcc
      601 acggtggtat ccaccacctg gtacttgacg gaactcgagc cacagttcag gacgagcgcc
      661 attccgatgt aattcacagc cacgtgtcta ctcctccgcc gcgttgtccg cagtcaattg
      721 ctgagcctgg atggccgtga tggccaccgt gttgacgatg tcttgaacca gcgcaccgcg
      781 gctgaggtcg ttgacgggct ttctgagacc ctgcaggacg gggccaaccg cgacggcgcc
      841 cgcgctacgc tgcacggctt tgtaggtgtt gtttccggtg ttgaggtcgg ggaagatcag
      901 gacggtggcg cgtcccgcca ccggggagtc gggcgccttc gacctggcga ccgacggttc
      961 gacggcggcg tcgtactgta tcggtccgtc aaccagcagg tcggggcggc gctcgcgcac
     1021 caggcgcgtg gcctcgcgga ccttgtccac gtctgatccg gtgcccgact cgcccgtcga
     1081 gtacgacagc atcgcgacgc ggggctcgat gttgaactgc tttgccgtct ccgcagacga
     1141 gatcgcgatg tcggcgagct gttcggccgt ggggtcgggg ttcaccgcgc agtcgccgta
     1201 gaccaacacg cggtccgcga gacacatgag gaagacggaa gagacgaccg agacgcccgg
     1261 gagcgtcttg atgatctgga acgagggcac gatggtgtgg gccgtcgtgt gcgccgcgcc
     1321 cgacaccatg ccgtctgcga gtccgagttc gaccatcatc gtgccgaagt agctgacgtc
     1381 ctgaactcgg tcgcgcgctg cctcttgggt cattcccttg tgcttgcgca tctcgtagta
     1441 cgcggccgcg aaacggtcga cgtgctcggg atcggagggt gacagaagct tggccgcgga
     1501 gaggtcgatt ccgagctctc cggcccttga ggtgatcgcg gccggatctc ccaggatcgt
     1561 gaggtccacc acgttgcgcg tcagcagggt gccggcggcg cgcaggatgc ggtcgtcttc
     1621 gccttcggga aggacgatgc gctgacgctt cgtgcgggca cggtcgagca gtgcgtactc
     1681 gaacatcagc ggcgtcacca cgtcggtggc ggccaggttc aggcgatcgg tgagttgcgt
     1741 gccgtcgacg tagtgctcaa acatgttgag ggccatgtcg accttgagga ccgactcgcg
     1801 agacaagcgg ccgcgggtgt tccagcaccg gtgcgctgtc tcaaaggtgc ccaggtcggt
     1861 ggcgatgatc ggcagggtcg gccccaagcc gtcaatgagg cgctggaccg agggcggcgg
     1921 gaggaatccg ccgttgacca cgatgcccgc gagcgcgggg aatccggcgg cggagtgcgc
     1981 ggtgagcgcg gcgagcaggg tgtcagccct gtcccccggg gcgatgatga gggcgccttc
     2041 ttcgaggcgg tcgagtaggt gctccactgc catggcggcg atcgtgatgg cgcgtacttc
     2101 acgcgacagg agggcctcgt cgccagcaat catcttcccg tcgatggcgt cgcgcagcgc
     2161 gccgagtgtc ggcgcgtaca gcagtggctc ttcgggaagg gcggccacgc cgatgtcttc
     2221 cggaagggcg gccttgatgg catcaacgtc gtcggggtcg cacctgttcg cgaaggccgc
     2281 gaccactttt gcgtggttct cgtccatggt ggagcgcgcc tggctcagca cctcggcgat
     2341 ttcggagggg gaccggccgg cgccgcgcac cacgagcgcg acgggcgccc ccaggtttgc
     2401 cgcgaccttg gcgttgagct caaattcggc ggggcccgcg acgtccgtgt agtccgtgcc
     2461 gacgatcacg acgacatcgc acttgcgcgc gacctcgtga tagcgcgtca cgatgcggga
     2521 tagggctgcc t
//
LOCUS       DASRKH010000352         2026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_59809, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000352 DASRKH010000000
VERSION     DASRKH010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59809"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="VFC06_05595"
     CDS             <1..527
                     /locus_tag="VFC06_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HZL81398.1"
                     /translation="LTTVLTWITNAFGPVVLPIFVTVACAVWGRVSGKWRDPLLLVGA
                     MVMSTMVAAVVKVIVERPRPAANLQVIPGLETSFSFPSGHTAGAATLVLVTAYLLWRH
                     RRTGRSLAWWLVTSVGIIALVGGSRLYLGYHFVTDVLAGACLGVVTLGLVVAASRWLD
                     LRQRRLAPTPGPAA"
     gene            complement(524..1813)
                     /locus_tag="VFC06_05600"
     CDS             complement(524..1813)
                     /locus_tag="VFC06_05600"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZL81399.1"
                     /translation="MANDGKIGLWPVVAIGVGGMVGGGIFAVLGLAVQLARGGTPLAF
                     AIAGAVALLTAYSYVKLSLAYPGRGGTVTFLNKAFGPGPLTGTLNVLLWLSYIVMLSL
                     YSYAFGSYGATFFPASPQGLAKHALITAAVVLIAGLNLLSAQVIGRAENWIVALKIAI
                     LAVFVGVGVFAIEPSRLAVGEWSPVIQVAAGGMIIFLAYEGFELIANTSDDIRDPQRN
                     LPRAYFIAVGFVVALYVLVAAVTVGSLDVQSIVDAKDFALAAAAKPFLGQAGFTLIAI
                     AAMLSTASAINATLYGSARLSYGIAKDSELPAELERKVWGRPIEGLLITAGVTVVIAN
                     VLDLGSIATIGSAGFLIIFGAVNVAAARRSHDIGARGWIAWTAAGACAVALGALVWQT
                     AVDSPAQLWILAGLLALAAGVEGAFQMTHRRPLRLTR"
BASE COUNT          335 a          671 c          719 g          301 t
ORIGIN      
        1 ggctcaccac cgtcctgacg tggattacca acgcctttgg gcccgtggtc ctgccgatct
       61 ttgtcaccgt cgcctgcgcc gtgtggggca gggtgtccgg caagtggaga gaccctcttt
      121 tgctcgtggg ggccatggtg atgtcgacga tggtggccgc agtggtcaag gtcatcgtcg
      181 agcggccgcg cccagcagcc aacctgcagg tcatccccgg cctcgagacg tcgttctcct
      241 tcccctccgg ccacaccgct ggcgcggcca cgctcgtgct ggtgaccgcc tacctgctgt
      301 ggcggcatcg gcgcaccggt aggtcgctcg cgtggtggtt ggtgacgtcg gtcgggatta
      361 tcgcgctcgt gggcggcagc cgcctgtacc tgggctacca cttcgtgacc gacgtgctcg
      421 caggcgcctg ccttggggtg gtgacgctgg ggctggtggt ggcggcgtct aggtggctcg
      481 acctcaggca gcgacgcctg gccccgacgc cgggccctgc tgcttagcga gtcagacgga
      541 gggggcgacg atgcgtcatc tggaaggccc cctcgacgcc ggcggcaagg gctagcaggc
      601 cggccaggat ccacaactgt gcgggactgt cgacggccgt ctgccacacc agggcgccga
      661 gcgcgacggc acacgcgccg gctgcggtcc acgcgatcca gccgcgagcg ccgatgtcgt
      721 gggagcggcg cgcggcggcc acgttcaccg ctccaaagat gatgaggaag ccagccgaac
      781 cgatggtcgc gatcgagccg aggtcgagca cgttcgcgat cacgacggtc acgccggcgg
      841 tgatgaggag accctcgata gggcggcccc acaccttgcg ctcgagctcg gcggggagtt
      901 cgctgtcctt ggcgatcccg tagctcagcc gcgcggatcc gtacagggtc gcgttgatgg
      961 ccgacgctgt ggacagcatc gcggcgatcg cgatgagcgt gaacccagcc tggcccagga
     1021 atggcttggc ggccgccgcg agcgcgaagt ccttggcatc gacgatgctt tgcacgtcga
     1081 gcgaaccgac ggtgaccgcc gcgaccagca cgtacagcgc caccacgaac cccaccgcga
     1141 tgaagtatgc gcggggcaag ttgcgctgcg ggtcgcgaat gtcgtcggac gtattggcga
     1201 tgagttcgaa gccttcgtac gccaggaaga tgatcatgcc gcccgcggcc acctggatga
     1261 cgggcgacca ttcgcccacg gcgagccgcg agggctcgat cgcgaacacc cccacgccca
     1321 caaagacggc gaggatggcg atcttgaggg ccacgatcca gttctctgct cgcccgatca
     1381 cctgagcact caacaggttg aggcccgcga tcagcacgac ggcggcggtg atgagggcgt
     1441 gcttggccag gccctgcggc gacgccggga agaaggtggc gccgtaactg ccaaaggcgt
     1501 acgagtacag cgacagcatc acgatgtagc tgagccacag cagcacgttg agggtgccag
     1561 tgaggggccc cggaccaaac gccttgttga ggaacgtcac cgtcccgccg cgacccgggt
     1621 acgcgaggga cagcttgacg tacgagtagg cggtgagcag cgcgaccgcg ccggcgatcg
     1681 cgaacgccag gggagtgccg ccgcgagcga gctgcacggc gaggccgagc accgcaaaga
     1741 tgccgccgcc caccatgccg cccacgccga tggccaccac gggccacaga ccgatcttgc
     1801 cgtcgttcgc catgaggcca tgttgccacg ggccccggct ccggctccgc ccgggtcgac
     1861 gcggctacgt ggctactagg gttcgaggcc cccgtagatg gtcggcagga actcgccaac
     1921 ggtggggtga atcgggagcc acgtagccgc gtcgacccgg gcggagccgg ggcccatggc
     1981 aacatggcct catggcgaac gacggcaaga tcggtctgtg gcccgt
//
LOCUS       DASRKH010000353         3259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_64472, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000353 DASRKH010000000
VERSION     DASRKH010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64472"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1163
                     /gene="secY"
                     /locus_tag="VFC06_05605"
     CDS             <1..1163
                     /gene="secY"
                     /locus_tag="VFC06_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HZL81400.1"
                     /translation="ISTNSAEGGPLQILNLFSGGALLQLSVFALGIMPYITASIMVQL
                     LRVLIPRFEALQKEGADGQAKLTQYTRYITVGFAALQSASYVTMARNGVFFPGCPAEI
                     VPDQSWNVITTMVLTMTAGTVLIMWMGERITERGIGNGMSLLIFTSVASSFPGAISTI
                     WQSTDRVRNTIVILIVIFAVIAAVVYVEQSQRRIPVQYAKRMVGRRMLGGSSTYIPLK
                     INMAGVIPVIFASSLLQLPYVIVQFAGDNPGAWVVWLQNNVTRTDAPLHISGYILLIV
                     FFAYFYTAITFNPTEVADNMKKYGGFIPGIRPGQPTADYLDYVLSRITAAGSIYLAIV
                     ALLPMLALVFLGLNTSIPLGGVTILILVGVGLDTVKRIQAQLEQRRYEGFLK"
     gene            1163..1729
                     /locus_tag="VFC06_05610"
     CDS             1163..1729
                     /locus_tag="VFC06_05610"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HZL81401.1"
                     /translation="MRLVLVGPPGAGKGTQATRLAYTYGVPAISTGHIFRTHVKRDTE
                     LGRKVQAYMSHGALVPDEVTNAMVRERLAEDDAAEGFVLDGYPRNPEQAVELDGMLAD
                     HGAALNAVVEITADSEEVTKRLLKRAEIEGRADDTEDVIRKRLSVYAETTAPVTAYYD
                     GQGLLVKVDGIAEVDVVTRRMVDALRVR"
     gene            1736..2545
                     /gene="map"
                     /locus_tag="VFC06_05615"
     CDS             1736..2545
                     /gene="map"
                     /locus_tag="VFC06_05615"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HZL81402.1"
                     /translation="MARGIEYKTRPQMRVMARAGRVVADALAAASTAAVVGATTSDVD
                     AAARRVIEAAGATSNFLGYYGFPAVSCVSVNDEVVHGIPGPRVLEDGDIVSVDCGAVI
                     DGWHGDSAVSLIVGKARGGQDKHLVEQTRLSLWAGIAALTTADRLDDVSGAIEDVAEA
                     AQLHPLLGYVGHGIGTAMHQAPEVLNYRTRGRHPKVKPGMCLAIEPMFVVGTGESTVL
                     DDDWTVVSADGSRAAHWEHSIAIHDEGIWVLTAHDGGASELARYGISPVAP"
     gene            2818..>3259
                     /locus_tag="VFC06_05620"
     CDS             2818..>3259
                     /locus_tag="VFC06_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81403.1"
                     /translation="MLDAARRELHRTHIALLAQSRNRTVRETIASRGDVPLGIQAALA
                     QDDATEVRSAIAGNAHTVRSLLEYLCLDKSHAVLASLLANANVPLEIVEGLAFHRRTE
                     VRRLAAARLNDPLRPPRVEDPQVEDAKFPELRERGTIAPAADVAD"
BASE COUNT          574 a         1034 c         1046 g          605 t
ORIGIN      
        1 gtatttcgac gaacagcgcc gagggcggtc cgctgcagat cctcaacctc ttctccggcg
       61 gagcgttgct tcagctgtcg gtcttcgcgc tcggcatcat gccgtacatc accgcgtcga
      121 tcatggtgca actgttgcgc gtcctcattc ctcgcttcga ggccctccaa aaggaaggcg
      181 ccgacggcca ggcaaagctg acgcagtaca cccgctacat cacggtgggg ttcgccgctt
      241 tgcagtcggc ctcgtacgtg acgatggccc gcaacggcgt gttcttcccc ggttgccccg
      301 cggagatcgt tccggaccag tcgtggaacg tcatcaccac gatggtgctc accatgaccg
      361 ctggcacggt actcatcatg tggatgggcg agcgcatcac cgagcgcggc atcggcaacg
      421 gcatgtcgct cttgatcttc acctcggtag cctcgtcgtt cccaggcgcc atctcgacca
      481 tctggcagag cacggaccgc gtgcgcaaca ccatcgtgat cctgatcgtg atcttcgctg
      541 tcatcgcagc cgtggtttac gtcgagcagt cgcagcgccg cattccggtg cagtacgcga
      601 agcgcatggt gggccgccgc atgctcggtg gttcgtcgac ctatatcccg ctcaagatca
      661 acatggccgg cgtgatcccc gtgatcttcg cgtcgtcgct gttgcagttg ccctacgtga
      721 tcgtgcagtt cgccggagac aaccccggtg catgggtggt gtggctgcag aacaacgtga
      781 cacggaccga tgcaccgctg cacatctcgg gctacatcct gctgatcgtg ttctttgcct
      841 acttctacac ggcgatcacc ttcaacccga ccgaggtcgc cgacaacatg aagaagtacg
      901 gcggcttcat tcccggcatt cgacccggac agccgacggc cgactacctc gactacgtgc
      961 tttctcgcat caccgcggcg ggttccatct atctggcgat cgtcgctttg ctgcccatgt
     1021 tggcgctcgt gttcctcggc ctcaacacgt cgattccgtt gggtggcgtc acgatcctga
     1081 ttctggtggg cgtgggtctc gacaccgtga agcggattca ggctcagttg gagcagcgac
     1141 gctacgaagg gttcctgaag taatgcgtct ggtgctcgtc ggcccccctg gggcaggcaa
     1201 gggaacgcag gcaacgcggc tggcgtacac gtacggcgtg cccgctatct ccacaggaca
     1261 catctttcgc acccacgtga agcgcgatac cgagttgggc cgcaaggtgc aggcatacat
     1321 gtcgcatggt gcactagtcc ccgacgaggt caccaacgcg atggtgcgtg aacgactggc
     1381 ggaagacgac gctgcggaag ggttcgtgct ggacggctat ccgcgaaacc cggagcaggc
     1441 cgtcgaactc gatggaatgc tcgccgacca cggtgccgcg ctcaatgcgg tcgttgagat
     1501 cactgcggat agtgaagagg tcaccaagag gcttctcaag cgtgcagaga tcgagggccg
     1561 tgccgatgac actgaagacg tgattcgcaa gcgcctgtcg gtctatgcgg aaacgaccgc
     1621 tccagtgacg gcctactacg acggtcaggg actcctcgtc aaggtggacg gcatcgccga
     1681 ggtcgacgtc gtgacccgtc gcatggtcga cgccctgcga gtccggtagg ggctcgtggc
     1741 gcgcggcatc gaatacaaga ccaggcccca aatgcgggtc atggcccgcg cgggacgagt
     1801 cgtggccgac gctcttgccg cggcgtcgac tgccgccgtc gtcggggcga cgacctctga
     1861 cgtggatgcg gccgcccgtc gggtgattga ggccgcgggt gccacctcga acttcttggg
     1921 ctactacggc ttccccgcag tgagttgcgt gtcggtcaat gatgaggtgg tccacgggat
     1981 ccccgggccg cgcgttcttg aggacggcga catcgtctcg gttgactgcg gcgcggtcat
     2041 cgacggatgg cacggcgact cggccgtgtc gttgatcgtc ggcaaggcgc gcggcggcca
     2101 agacaagcac ctggtcgagc agacccggct gtcgttgtgg gcgggcatcg ccgctctcac
     2161 gaccgccgat cgacttgacg acgtctcagg ggcgatcgaa gacgttgccg aggccgcgca
     2221 actccacccg ttgttgggct atgtggggca cggcatcggg acagcgatgc accaagcgcc
     2281 ggaagttctc aactaccgca ccagagggcg tcaccccaag gtcaagccag gcatgtgtct
     2341 ggctattgag cccatgttcg tggtgggaac gggggagtcc accgtgctcg acgacgactg
     2401 gactgtggtg tcggcagacg gatcgcgcgc cgcccattgg gagcactcca tcgccattca
     2461 cgacgaggga atctgggtat tgaccgccca cgacgggggc gcgtctgagc tggcccggta
     2521 cggcatttct cccgtcgctc cctagtccgg ccaccctgag tcacgcgccc cccgacgtgg
     2581 ggccccgcgt agtgtcgtga tggggtgttt tgcggcgatc cttgtgagtg ttctcacaaa
     2641 tgaggtcaag aagtggcggg acggcatgtc cgaaatgtat gacttatgca tccgccggcg
     2701 gcggggcacc cgacggacca cagaaagatg ggtcggggcg actaccgccc cgctcgcaaa
     2761 ccaggggaca ccgacatggc acgtggattg gcacaagcgc caagtgcaag tgtcgaagtg
     2821 ctcgacgccg cgaggcgcga attgcaccgc actcacatcg cgttgctcgc ccagtcccga
     2881 aatcggacgg tccgtgagac tatcgcgagc cgcggcgacg ttcccctcgg catccaggcg
     2941 gcgcttgccc aagacgatgc cactgaggtg cgctcggcca tcgcgggaaa cgcccacacc
     3001 gtgaggtcct tgctcgagta cctttgcttg gacaagagcc acgcggtgtt ggcctcgttg
     3061 ctcgccaacg ccaacgtccc tttggagatc gtcgagggcc tggcctttca ccgtcgcacc
     3121 gaggtgcgaa ggctcgcggc cgctcgactg aatgacccgt tgcggccgcc ccgcgtcgaa
     3181 gaccctcaag tcgaggacgc caagttccct gaactgaggg aacgcgggac catcgcacca
     3241 gctgcagatg tggcagacg
//
LOCUS       DASRKH010000354         4021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_15379, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000354 DASRKH010000000
VERSION     DASRKH010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15379"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..885
                     /locus_tag="VFC06_05625"
                     /pseudo
     CDS             64..885
                     /locus_tag="VFC06_05625"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            975..2174
                     /locus_tag="VFC06_05630"
     CDS             975..2174
                     /locus_tag="VFC06_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HZL81404.1"
                     /translation="MSPISLAEPTGRYLSFAEREEIALLRVQGVGVREMARRLGRDPG
                     TISRELRRNAATRGGKQEYRALVAQWKAQQAAKRPKTAKLAGDPRLRQYVHERLSGSI
                     RGPGGALVSGPDTPAWKGLNKPHRQDRRWALAWSPQQISHRLMIDFPDDESMRISHEA
                     IYQSLFIEGRGALKRELVTCLRTGRALRQPRERSRNKPQGHVTADVVLSERPAEAADR
                     AVPGHWEGDLIIGTGRSAIGTLVERSSRSTILVHLPRLEGWGDTPYVKNGKSLGGYGA
                     VAMNAALATSMTRLPEQLRKTLTWDRGKELSGHAQFALETGTKVYFADPHSPWQRPTN
                     ENTNGVLRQYFPKGTDLSRWSAEDLEAVAFAVNNRPRKVLGWKTPVEVFEEQLQLLQQ
                     PGVATTD"
     gene            2227..2481
                     /locus_tag="VFC06_05635"
                     /pseudo
     CDS             2227..2481
                     /locus_tag="VFC06_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611981.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(2533..3333)
                     /gene="istB"
                     /locus_tag="VFC06_05640"
     CDS             complement(2533..3333)
                     /gene="istB"
                     /locus_tag="VFC06_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HZL81405.1"
                     /translation="MTAATSASIYQQLKGHLAELKLADAAEALPRVLDQAQAEGWSLT
                     QTLEHLLAVEVTATDARRLAGRFRFANLPTGATLDDFDLDAASGIDQSLLAELGTCHY
                     LDTATNVLLIGPPGVGKTHIATGLGHAAVKAGYRTYFTSAADLAARCHRAAIEGKWGT
                     MMRFFAGPTLLLIDELGYLPLPAEAASALFQVINQRYLKTSIVITTNRPVGAWGEILG
                     DTTVAAAMLDRLLHRSVVVTLDGGSYRLRNHAAAADELRRVTTGTNLR"
     gene            complement(3366..>4021)
                     /locus_tag="VFC06_05645"
     CDS             complement(3366..>4021)
                     /locus_tag="VFC06_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HZL81406.1"
                     /translation="ACRPRSGNRKGVVEKNNHTAAQRWWRNLPDELTPEQAQQSLTTF
                     ATSQDGRRREGRDGSTTAAAMFAAERLAPLPPTLFPVVVVEDRLATRQALIDWRGNRY
                     SVPPELAATRVVVHQRLGAATIDIATVSGVVLARHRIAEPGLGVTIRDSGHVTALEAI
                     ALASAPPGRPHRKKERIPPGTGARAAAAELAGTPAATTTVINLAAYEQAAKNRNTLQ"
BASE COUNT          663 a         1226 c         1428 g          704 t
ORIGIN      
        1 ggcgcggctg cgtaaagaga actcggagct gaagatggac cgggcgtttc taaaaaaagc
       61 gtcgctcttc ttcgcccagg aagcgtcgga tacgaacgag aagcgttcgc actgatgcac
      121 gcggagaaga gtagtttcac ggtctcacgc atggcacgac tgctggaggt gtcccggtct
      181 ggttactacg catgggtcca ccgggagccc tcgacactgg cgatccgtcg tgaacggatc
      241 gaggccaaag tggtgtggtt ccacggcgag tctgacgagg tgtccggctc accgcgaatc
      301 ctgatggatt tgcgcgagga tggcgagatc atcagccgca agacggtcgc tcacgcgatg
      361 cgccggctgg gtctgcgggg tgtcagcccc aagaagtggc gcaccaccac cgtcactgac
      421 gcgtcggatt cctaccccgc tgacgcggtc aaacggacct gggacaccgg cgtgttgaac
      481 agtgtgtggg tgggtgacat cacgtatctg cgcacctggg aagggtggct ctacttggcg
      541 accgtgatcg atgcctgctc gcgtcgggtg atcggttggg cgatcgatga gcacatgcgc
      601 actgacctcg tcgaggatgc cctccgaatg gctatcacct tgcgcggcga cctgcccgac
      661 aaggtcgtgt ttcacaccga ccggggcacc caaggcggat tcaactggtc gtcgcaacac
      721 cttgatcttg gaggtgtgcg atgggacgta gtgggcaaca gatgccggcg gtgccggagg
      781 gtggacggcg acagtgggca gcggatcggg cgctgcggcc gccgatgcgt tcgccgggtc
      841 ggccagagcc gtcgcgtgcg gtgcaacgtg agttctggcg gctgatcgcg tccggcgtga
      901 cgacggtcga ggctgccgag ggtgttggcg tgtcatggcc tgtcgcgtct cgctggttcc
      961 gccacgctgg cggcatgtcg ccgatcagtc tggccgagcc tacgggacgg tacttgtcgt
     1021 ttgccgagcg ggaagagatc gcgctgctac gcgttcaggg tgtcggtgtg cgtgagatgg
     1081 cccgcaggct cggacgggat ccgggcacga tctcacgtga gttgcgccgc aacgcggcga
     1141 ccaggggcgg gaagcaggag taccgggcat tggtcgcgca gtggaaggcg cagcaggccg
     1201 caaagcgccc caagactgcg aagctcgctg gcgatcctcg gttgcgccag tacgtgcacg
     1261 agcggctgtc tggcagtatc cgcgggcccg gcggcgcgct cgtcagtggg cccgatacgc
     1321 ctgcgtggaa ggggctgaac aagccgcacc ggcaggatag gcgctgggca ctggcatgga
     1381 gcccgcaaca gatctcgcac cggctcatga tcgacttccc cgatgatgaa tccatgcgta
     1441 tcagtcatga agcgatctac cagtcgctgt tcatcgaggg ccgcggcgcg ctcaagcgtg
     1501 aactggtcac atgccttcgc actggccgtg cactacgcca gccgcgggag agatcacgca
     1561 acaagccaca agggcatgtc accgccgatg tcgtccttag tgaacgtcct gctgaggccg
     1621 ccgatcgcgc cgtgcctggg cattgggagg gggatctgat catcggcacc ggtcgctccg
     1681 cgatcggcac cctcgtggag cgctcgagtc ggtccacgat cttggtgcac ctgccgcgcc
     1741 tggagggctg gggtgacacg ccttacgtga agaacggtaa gtcgctgggt ggctacggcg
     1801 cagtcgcgat gaatgcagcc cttgcgacct cgatgacccg ccttcctgaa caattgcgca
     1861 agaccctcac ctgggaccgg ggcaaagaac tatcgggtca tgcccagttc gcgctcgaga
     1921 ccggcaccaa ggtgtacttc gcagatccgc actcgccctg gcagcgaccc acgaacgaga
     1981 acacaaatgg cgtgctgcgc caatacttcc ccaaaggcac cgacctgtcc cggtggtccg
     2041 ctgaggacct cgaggccgtc gcgttcgcgg tcaacaaccg accccgcaag gtcctcggct
     2101 ggaagacccc ggtcgaggtc ttcgaagagc aactacagtt gctccaacag cccggtgttg
     2161 caacgaccga ttgaacccgc ccaatatgca tcgacgcaga tcaccgcgtt cgccgccgtc
     2221 aatggtctga ccaggtcgat gggctcgacc gggatttgct gggataacgc gatggccgag
     2281 tcgttcttcg cgaccctgaa gaccgagttc tactaccgtc gggtctggcc cacgaaaaag
     2341 cgtgcaaaga ccgccgtcgg ggcctggatc gaggaccgct acaaccgcaa tcgtcggcac
     2401 tcctcaatag gccagatcag ccccgtggcg ttcgagatgc aacacttcaa ccagaccgcg
     2461 gcatttcaag aagccgcata accccgtgtc caccaaacgg gggcaaggcc atccccaggt
     2521 cgcggtgtca ggttagcgca ggttcgtgcc ggttgtgacg cggcgtagtt cgtcggctgc
     2581 ggcggcgtgg ttgcggagcc ggtaggagcc tccgtcgagg gtgacgacga cggatcggtg
     2641 gaggagtcgg tcgagcatcg cggcggcgac ggtggtgtcg ccgaggatct cgccccaggc
     2701 gccgacgggc cggttcgtgg tgatcacgat tgacgttttc aggtagcgct ggttgatgac
     2761 ttggaacagg gctgatgctg cctcggctgg cagcggcaga tacccgagtt catcgatcag
     2821 gaggagcgtc gggccggcga agaaccgcat catggtgccc catttgccct cgatcgcggc
     2881 cctatggcag cgcgcggcga ggtcggcggc ggaggtgaag taggtgcggt aaccggcctt
     2941 cacggcggcg tggccgaggc cggtggcgat gtgtgtcttc ccgaccccgg gcgggccgat
     3001 gaggagcacg ttcgtcgcgg tatccagata gtggcaggtg ccgagttcgg cgaggaggga
     3061 ctggtcgatg ccggacgcgg cgtcgaggtc gaagtcgtcc agggtggcgc cggtgggaag
     3121 gttcgcgaac cggaacctgc ccgcgaggcg tcgggcgtcg gtagcggtga cctcgacggc
     3181 gagcaggtgc tcgagtgtct gggtcagcga ccagccctcg gcctgcgctt ggtcgaggac
     3241 gcgcggcaac gcctcggcgg cgtcggcaag ttttagctcg gccaggtggc ccttcagttg
     3301 ttggtagatg ctcgcggacg tcgctgcggt catcgttgtc gaggcggttt ggggtgtcgg
     3361 tgtcgtcatt ggagggtgtt cctattcttc gcggcctgct cgtaggcggc caggttgatc
     3421 acggtcgtgg tggcggccgg ggtgccggcc agttcagcgg cggcggcgcg ggcgccggtc
     3481 cccggtggga tgcgttcctt cttccggtgc gggcgcccgg gaggcgccga cgccaaggcg
     3541 atcgcctcga gtgcggtgac gtgtccgctg tcacggatcg tcacccccag cccaggctcc
     3601 gcgatccggt gtcgcgcgag caccacgccg gacacggtgg caatgtcgat tgtcgccgcg
     3661 ccgagacgct gatggacgac cacgcgggtc gctgccagct ccggcgggac cgagtagcgg
     3721 ttgccgcgcc agtcgatcaa cgcctgccgt gtcgcgaggc ggtcctcgac caccacgacg
     3781 gggaacaggg tcggtggcaa cggcgcgagc cgctccgcgg cgaacatcgc cgcggcggtc
     3841 gtcgatccgt cgcggccctc gcggcgccgc ccgtcctgcg aggtcgcgaa cgtcgtcagg
     3901 ctctgctgcg cctgctcggg agtgagttcg tcgggcaggt ttcgccacca ccgttgcgcg
     3961 gcggtgtggt tgttcttctc caccacccct ttacggttcc cggacctcgg tcggcaggcg
     4021 a
//
LOCUS       DASRKH010000355         2031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_42598, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000355 DASRKH010000000
VERSION     DASRKH010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42598"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..1750
                     /locus_tag="VFC06_05650"
     CDS             170..1750
                     /locus_tag="VFC06_05650"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HZL81407.1"
                     /translation="MATVAAMQPGWNLGNTLDAIPDETAWGNPLTTRELIHHVRSQGY
                     NSIRVPVTWNGHFGPAPGYTIDPAWLARVEQIVDWSLDEGLYVMLNLHHDSWEWADSY
                     PTDKAGVTARYRALWDQLATTFRDHSSKLVFESINEPAFTDATDAQGDVYVQELNTDF
                     VERVRRSGGGNATRLLVLPTLHTSSEQPRLDALAATIDQLDDPNIAATVHFYGWWPFS
                     VNIAGGTRYSTDVEQDLVSSFDRVTSTFVERDIPVIIGEWALLAWDHTRPGIIERGEF
                     LKYIEAVGHHARSRGLTTMVWDAGQFLDRNTLQWRDAGVHAMMRSSWTARSGTASSDQ
                     VYLPRSGTISAASLTLNLNGTTFQGLRHGSTDLARGSDYTVNGNQLTLTASALTRLAG
                     TRQYGVNATIEAQFSQGVPWQISIITADRPTLGAATGTTSALVIPTQFRGDTLATMEA
                     RYDDGSNAGPASWTSYKEFWSNFQPDAAAGTILLKQDFFNEVQDGRRVTLTFHFWSGA
                     QVTYTVTRSGTSVVGGPA"
     gene            1838..>2031
                     /locus_tag="VFC06_05655"
     CDS             1838..>2031
                     /locus_tag="VFC06_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81408.1"
                     /translation="MTALVSDDSDRPAMPSSTHELRTITDKIDVRPVGQAHVDRDVEF
                     TAFVVAAKGDLGKTAWLLTG"
BASE COUNT          347 a          752 c          657 g          275 t
ORIGIN      
        1 caacggctcc tggaccggca gcaacgccgc accgttgagg gtgaacgcgg tgggcgcggc
       61 gtgcaccggg caggtgggcg ggacgccgtc accgaccgcg tcaccgacgg ccacggcgac
      121 ggcgacggcg acggccaccc cgaccacgtc gccgcgaccg gtcgacgcca tggcgaccgt
      181 cgccgcgatg cagcccggct ggaacctggg caacaccctc gacgcgatcc ctgacgagac
      241 cgcgtggggc aacccgctga cgacccggga gctgatccac cacgtccggt cgcagggcta
      301 caacagcatc cgtgtcccgg tgacctggaa cgggcacttc ggcccggctc ccggctacac
      361 gatcgacccg gcctggctcg ccagggtcga gcagatcgtc gactggtcgc tggacgaggg
      421 tctctacgtg atgctcaacc tgcaccacga ctcgtgggag tgggccgact cgtacccgac
      481 cgacaaggcc ggcgtcacgg cgcggtaccg ggccctgtgg gaccagctcg ccaccacgtt
      541 ccgcgaccac tcgtccaagc tcgtgttcga gagcatcaac gagcccgcgt tcacggacgc
      601 cacggacgcg cagggtgacg tgtacgtgca ggagctgaac accgacttcg tcgagcgcgt
      661 gcgccgctcc ggcggaggca acgccacccg gctgctcgtg ctgccgaccc tgcacaccag
      721 cagcgagcag ccccgcctgg acgcgctggc cgccacgatc gaccagctcg acgacccgaa
      781 catcgcagcg accgtccact tctacgggtg gtggccgttc agcgtgaaca tcgccggcgg
      841 cacgcgctac agcaccgacg tcgagcaaga cctcgtcagc tcgttcgacc gggtgaccag
      901 cacgttcgtc gagcgcgaca tccccgtcat catcggggag tgggccctgc tcgcctggga
      961 ccacacccgg ccgggcatca tcgagcgggg cgagttcctc aagtacatcg aggcggtcgg
     1021 gcaccacgca cgcagccgcg gcctgacgac gatggtctgg gacgccgggc agttcctcga
     1081 ccgcaacacg ctgcaatggc gggacgcagg cgtgcacgcc atgatgcggt cgagctggac
     1141 ggcccggtcc ggcaccgcgt cgtccgacca ggtgtacctg ccccgcagcg gcaccatctc
     1201 cgccgcgtcg ctgacgctca acctcaacgg caccacgttc caggggctgc ggcacggcag
     1261 cacagacctg gcccgcggga gcgactacac cgtcaacggc aaccagctca ccttgaccgc
     1321 ctccgcactg acccgactcg ccggcacccg gcagtacggc gtcaacgcca ccatcgaggc
     1381 ccagttctcc cagggcgtgc cctggcagat cagcatcatc accgccgaca ggccgaccct
     1441 gggcgccgcg accggcacca ccagcgcgct cgtcatcccc acgcagttcc gcggggacac
     1501 gctcgccacc atggaggccc ggtacgacga cggctcgaac gccggacccg cctcgtggac
     1561 gtcgtacaag gagttctgga gcaacttcca gccggatgcc gccgccggga ccatcctgct
     1621 caagcaggac ttcttcaacg aggtccagga cggccgtcgc gtcacgctca cgttccactt
     1681 ctggagcggc gcgcaggtga cctacaccgt caccaggtcg ggcacgtcgg tggtcggagg
     1741 tccggcctga ccctgacgtc gagctgcggg acgcgctgac gacgaactgg ttccgatctc
     1801 gtggcaacct tcgcctgccg ccatgcgtgt gaacggtgtg actgcactcg tgtccgacga
     1861 ctcagaccgt cccgcgatgc cgtcgtcgac gcacgagctg cgaacgatca cggacaagat
     1921 cgacgttcgc cctgtgggcc aggctcacgt cgatcgcgac gtggagttca cggccttcgt
     1981 cgtcgccgcg aagggcgacc ttggcaagac ggcctggctg ttgaccggtg a
//
LOCUS       DASRKH010000356         2603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_46800, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000356 DASRKH010000000
VERSION     DASRKH010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46800"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..1464
                     /locus_tag="VFC06_05660"
     CDS             163..1464
                     /locus_tag="VFC06_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HZL81409.1"
                     /translation="MRTLRIWRTLLGVEKTFVEDIDLDLESRILVASVRPMVSKRNRC
                     GACHKRSPRYDAGDGRRRWRGLDVGSIQVHLEADAPRVSCRVHGVTVAAVPWARHQSG
                     HTLFFDDQVAWLATQTSKTAITVLMRVAWRTVGAIITRVWADTGKQFDQFAGLTRIGI
                     DEISYKRGHKYLTCVVDHDSGRLVWAAPGQDKATLATFFDALGPERSAQITHVSADGA
                     AWIASVVKEKAPNAIRCADPFHVVMWATDALDEVRRSAWNDARKAARQNEARRTRGRP
                     AADAPARPDSARAAGIKNCRYALWKNPENLTEKQQAKLAWIVQTDPRLARAYYLKEGL
                     RVIFKLPLAEATEALDKWVGWARRCRIPAFVKLQKSIVKHRTAILASIEHGLSNGRVE
                     SMNTKIRLITRIAFGFKSPDALIALAMLSLGGHKPVLPGRN"
     gene            complement(1477..1704)
                     /locus_tag="VFC06_05665"
     CDS             complement(1477..1704)
                     /locus_tag="VFC06_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZL81410.1"
                     /translation="MRTTHTVNTAMVIAQTMLRAPSTISRELRRNQTDTGTYEPYAAH
                     RTAAGRRPRPKAVKLTAGSSLHTFVQEKRLS"
     gene            complement(2194..>2603)
                     /locus_tag="VFC06_05670"
     CDS             complement(2194..>2603)
                     /locus_tag="VFC06_05670"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZL81411.1"
                     /translation="RMITRTFRTVVLAAVAAVALVACSSGSAEPPAFDGSHVGASAFA
                     LAVEQPGVTVVDVRTPAEFADGHLPGAVNINVEDPAFPNAIAALDATADYAVYCRSGN
                     RSRAAMEYMTQVGVTQTVGLEGGITAWKGAVER"
BASE COUNT          439 a          882 c          838 g          444 t
ORIGIN      
        1 cctttgcatg gctcatgaaa gcgaacacta acaaagtggg gcaaaactgg ctctcctgct
       61 gcttccgtgg gtcttttcgg agcgccttaa cggccgcacg ccgctctccg cctaggtttc
      121 tgactgttca aagaacagaa actttagggc gggaagagcg gcgtgcgaac tctaagaata
      181 tggcgaaccc tccttggtgt cgagaagacg tttgttgagg acatcgatct cgatctggag
      241 agcaggattc tggtcgcgtc ggtgcgtccg atggtgtcga agcggaaccg atgcggcgcc
      301 tgccacaagc gcagcccgcg ttacgacgcc ggtgatgggc ggcgccgttg gcgagggttg
      361 gacgtgggct cgattcaagt tcatctcgag gccgacgcgc cccgtgtgtc ctgccgtgtc
      421 cacggcgtga ccgtggcagc tgttccgtgg gcgcgacacc agagcggtca caccctgttc
      481 ttcgacgacc aggtcgcgtg gctggccacg caaacgtcca agaccgccat caccgtcctg
      541 atgcgcgtcg cgtggcgcac ggtcggagcg atcatcaccc gggtctgggc cgacaccggc
      601 aagcagttcg accagttcgc tggcctcacc cggatcggga ttgacgagat ctcctacaag
      661 cgcggccaca agtatttgac ctgcgtcgtg gaccacgatt ccggccgtct cgtgtgggcc
      721 gcgccgggtc aggacaaggc caccctcgcg acgttcttcg acgcgttagg cccggaacgc
      781 tcggcccaga tcacgcatgt ttccgcagac ggtgcggcct ggatcgccag tgttgtcaag
      841 gagaaagccc ccaacgcgat ccgctgcgcc gaccccttcc acgtcgtaat gtgggccacc
      901 gacgcgctcg acgaagtgcg gcgcagcgcg tggaacgacg cccgcaaagc ggcccggcag
      961 aacgaggctc gccgcactcg tggtcggccc gccgccgacg ctcccgcacg cccggacagc
     1021 gcccgagctg ctggtatcaa gaactgccgc tacgccctct ggaagaaccc cgaaaacctc
     1081 accgagaagc aacaagcgaa gttggcctgg atcgtgcaga ccgacccccg gctggcacgc
     1141 gcttactacc tgaaagaagg cctccgcgtg atcttcaaac tgcccctcgc cgaagctacc
     1201 gaggccctcg acaaatgggt tggctgggcc cgacgctgcc gcatccccgc gttcgtgaaa
     1261 ctgcagaaga gcatcgtgaa gcaccgcacc gcgatcctcg cctcgatcga gcacggcctc
     1321 tccaacggcc gcgtcgaatc catgaacacc aagatcagac tcatcacccg catcgccttc
     1381 ggcttcaaat cacccgacgc cctcatcgcc ctcgccatgc tcagcctcgg cggccacaaa
     1441 ccagtgctcc ccggccggaa ttaacccacg gttacgtcag gagagccgct tttcctgcac
     1501 gaaggtgtgc agcgacgacc ccgcagtcag tttcacggcc ttcgggcggg gccggcgccc
     1561 agcggccgtg cggtgggcgg catagggctc atacgtgccc gtgtcggttt ggttgcggcg
     1621 cagctcccgg ctgatcgtcg acggcgcccg cagcatggtc tgcgcgatca ccatggccgt
     1681 gttgacggtg tgggtagtgc gcacgtggtc aacggacgcg catatccagc atgtgcacgt
     1741 gcaccccgcg tggttcatcc ccgtggtcgg caacatcgtg gtgccgctag ctggcgtcgc
     1801 tcacgcgccg gtggagctgt cgtggtactt cttcggcgtc ggcatcgtct attggctggg
     1861 aatcatgccg atcgtgctca ccagactgtt cgtgctcggc acaatcccac cccgcctcgc
     1921 acccaccctg gccatcatgg tggcccctcc cgccgtcggc gcactctccc gggtgcggct
     1981 cggcggcgac tggtcggacc ctcttgggcg tacacgttcc ccctcgcgac ggccgccgcc
     2041 gcgttcgtcg cggcgtacga gtcgggagag ggcgcgttct acgcgtgggc gggcggagtc
     2101 accttgggca tcgcctcggc gattgtgctt accctgggcg ttcgcacggt ccgcggcttt
     2161 gccacgggcg agctcttgca gcctgaggac tagctagcgc tcgacggcgc ccttccaggc
     2221 ggtgatgcca ccctcgaggc ccacggtctg cgtcacgccg acctgagtca tgtactccat
     2281 ggccgcgcgc gagcgattgc ccgagcggca gtacacggca tagtcggcgg tggcatcgag
     2341 tgcggcgata gcgttcggga acgccgggtc ctccacattg atgttgacgg ccccaggcag
     2401 gtggccgtcc gcgaactcgg cgggagtgcg gacgtcgaca acggtgacgc cgggctgctc
     2461 gacggcgagc gcgaaggcgc tcgcgcccac gtgcgagcca tcgaaggcgg ggggctcagc
     2521 agaccccgac gagcaggcta cgagagcgac ggccgccacg gcggcgagga cgacggtgcg
     2581 aaaagtgcgt gtgatcatcc tcc
//
LOCUS       DASRKH010000357         2207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_46833, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000357 DASRKH010000000
VERSION     DASRKH010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46833"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..671)
                     /locus_tag="VFC06_05675"
     CDS             complement(51..671)
                     /locus_tag="VFC06_05675"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HZL81412.1"
                     /translation="MAILLEIHPVDPQPRLISMVVDVLRDGGLIAYPTDACFALGAVL
                     GNQQAKERIRQIRHLDERHHFTLVCHDFAQLGQLVRLSNSVFRAVKAATPGPYTFILP
                     ASPEVPRRLQHPRKKTVGVRIPEHRITQAILAELGEPLLSSTLLLPGEEEPLTTGVDI
                     RDALDHLVDLVVDGDAAGSEPSTVVDFSSGSPEVVRVGAGDPTRFS"
     gene            complement(697..852)
                     /locus_tag="VFC06_05680"
     CDS             complement(697..852)
                     /locus_tag="VFC06_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81413.1"
                     /translation="MSTGDDLSTLAVARGQGLAAACLARTIRLGFGVRRTFASMQRDA
                     VEPTATG"
     gene            complement(906..>2207)
                     /locus_tag="VFC06_05685"
     CDS             complement(906..>2207)
                     /locus_tag="VFC06_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-binding domain-containing protein"
                     /protein_id="HZL81414.1"
                     /translation="EERREIERALRRGDLLALATTNALELGIDISGLDAVLIAGWPGS
                     RASLWQQAGRAGRAGADGLAVFIASDDPLDNFLVHHPDAIFGAAVEATTFDPANPYVL
                     GPHLCAAASELPITRADFALFGLADEALLTALTGRGLLRARPTGWYWNLALGGRAQDL
                     TDLRGSGGGEVQVVESLTGAVLGTVGAGNADTTVHPGAVYIHQGRVHVVEELADDVAL
                     VRHENPGYRTRTQSVSAIRILETERAWAWGQGPGAVAWGFGPVEVTSQVTGYDRLLPP
                     AMEIVGHYTLAMPERLLPSKAVWWSLPEEACLAAGLTPEVLPGALHAAEHASIGMLPL
                     LATCDRWDIGGVSTANHPDTGAPTVFVYDGYPGGAGFAERGSRVARQWLEATLGAIEG
                     CRCEDGCPACIQSPKCGTGNAPLSKDGAIHLLRLVLARGGP"
BASE COUNT          301 a          787 c          829 g          290 t
ORIGIN      
        1 acccccacgg gccgtgttgc tcccgcgcgg cggggttgct cccgcgcgcg tcaggagaac
       61 cgggtgggat ccccggcccc gacgcggacc acctcggggc tgccactgga gaagtcgaca
      121 acggtggacg gctcgctgcc ggcggcatcc ccgtcaacga cgaggtccac caggtggtcg
      181 agggcgtccc tgatgtccac ccccgtggtc aagggctcct cctccccggg cagcaacagc
      241 gtggaggaca gcagcggctc ccccagctcc gcgaggatgg cctgggtgat gcggtgctcg
      301 gggatgcgca cgccgacggt cttcttccgc gggtgctgca ggcggcgcgg cacctccggg
      361 gaggcgggga ggatgaaggt gtacggcccg ggcgtggccg ccttcactgc ccggaagacg
      421 gagttgctga ggcgcacgag ctggccgagt tgcgcgaagt cgtggcacac gagcgtgaag
      481 tggtggcgct cgtccaggtg gcggatctgg cggatccgct ccttggcctg ctggttgccc
      541 agcaccgcgc cgagggcgaa gcaggcgtcc gtggggtagg cgatgaggcc gccgtctcgc
      601 agcacgtcca cgaccatgga gatgaggcgc ggctgcgggt ccaccgggtg gatctcgagg
      661 agaatggcca tggtccaccg tagcgggcgc cggccctcac ccggtggcgg tcggctccac
      721 cgcatcccgc tgcatgctgg cgaacgtcct cctgacgccg aagcccagcc ggatcgtccg
      781 cgccaggcag gcggccgcga gaccctgccc ccgggcgacg gccagcgtgg agaggtcgtc
      841 tcctgtgctc atgcatccat cgtgcgggac cacggtcgtg cacgtccacg cctttccggg
      901 tgcggtcacg gtccacccct cgcgaggacg agccggagga ggtggatggc gccgtccttg
      961 gacagggggg cgttcccggt gccgcacttc ggggactgga tgcacgcggg gcagccgtcc
     1021 tcgcaccggc acccctcgat cgcgccgaga gtcgcctcga gccactggcg tgccacccgc
     1081 gatccgcgct ccgcgaagcc cgccccgccg gggtacccgt cgtacacgaa caccgtcggg
     1141 gcgcccgtgt ccgggtggtt cgcggtggag acgccgccga tgtcccaccg gtcacaggtg
     1201 gcgagcagcg ggagcatgcc gatcgaggcg tgctcggcgg cgtgcagcgc cccgggcagc
     1261 acctcggggg tgagcccggc cgcgaggcat gcctcctccg ggagcgacca ccacacggcc
     1321 ttcgagggga gcagccgctc gggcatcgca agggtgtaat gccccacgat ctccatcgcg
     1381 ggaggcagga gccggtcgta ccccgtcacc tggctcgtga cctccaccgg gccgaagccc
     1441 caggccacgg cgccgggacc ctgcccccac gcccaggcgc gctccgtctc caggatccgg
     1501 atcgccgaca ccgactgcgt gcgcgtgcga tacccggggt tctcgtgccg cacgagcgcg
     1561 acgtcgtccg cgagttcctc caccacgtgg acccggccct ggtggatgta gaccgcgccg
     1621 ggatggacag tggtgtcggc gttgcccgcg cccaccgtgc ccagcacggc cccggtgagg
     1681 gactccacca cctgcacctc gccgccgccg gagccgcgca ggtccgtgag gtcctgggcg
     1741 cggccaccga gcgcgaggtt ccagtaccag ccggtggggc gggcccggag caggccgcgc
     1801 ccggtgaggg cggtgaggag cgcttcgtcc gcgaggccga agagcgcgaa gtcggcccgg
     1861 gtgatcggca actccgacgc cgcggcacag agatggggcc cgagcacgta cgggttggcc
     1921 gggtcgaagg tggtggcctc gacggccgcg ccgaagatcg cgtccgggtg gtgcaccagg
     1981 aagttgtcca gcgggtcgtc tgaggcgatg aagaccgcga ggccgtcagc tcccgcgcgg
     2041 ccggccctgc cggcctgctg ccacagcgag gcgcgcgatc ccggccagcc ggcgatgagg
     2101 acggcgtcga gcccggagat gtcgatcccc agttccaggg cgttggtggt ggcgagcgcg
     2161 agcaggtcgc cccgccggag cgcgcgctcg atctcgcgcc gctcctc
//
LOCUS       DASRKH010000358         3885 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_2013, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000358 DASRKH010000000
VERSION     DASRKH010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2013"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VFC06_05690"
     CDS             complement(<1..192)
                     /locus_tag="VFC06_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81415.1"
                     /translation="MTYKQPLPGGRLPQSNHGRAGAGLGSNQAQTKFGKQGEIGASGE
                     RHFAQMLQRAALDEVYDIFY"
     gene            complement(312..1310)
                     /locus_tag="VFC06_05695"
     CDS             complement(312..1310)
                     /locus_tag="VFC06_05695"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HZL81416.1"
                     /translation="MADVKAKYSERVEAMVPGGGKGVDLPSELLDAWADMKVQKESPS
                     SFASFLECPTKWFVERHLGPLHEKTGKTILGITDFTRQPANQWGVGGSLAHRALEVLY
                     TEPPGFRTREVLEDIYDHAWDCLKAGDIDDGIVSKSLVTEWESVLANLTDPPKSFKSF
                     FRTTYRDLALAIFDMETPPRVNVLANEQIVRLQQGRLNVFGKIDRTDRTLNGLEKIVD
                     YKTGKNPTGEVSVFNKTFLPSGIYALARHRARADNISALPVAGVQLVYLKSGERFSIK
                     ATPEMYRDVEEVLDMIGNETAAIADRGYVPTSPAASKDDAPCRFCKIRDLCPDWNE"
     gene            complement(1319..2191)
                     /locus_tag="VFC06_05700"
     CDS             complement(1319..2191)
                     /locus_tag="VFC06_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81417.1"
                     /translation="MANIIDVARALRDPVEGYEALVPPVVAAQLSEVSPTAVIGFIAG
                     ALVHQAAVHVPDSEKTGSKVFKGKVLEVKHVDRSSNGRLLGKKAIIVLDSGGDKDEEL
                     STHWVEFMGETDPERLRDPKVAFDMALSQANEADALALVGKDVFVRKAFAKTQGTVRG
                     GGDTVRYLASIRENFSNGGDHDSDSRGSSRSSGGASKSDSAKSDSAEVTVATIRTACK
                     SGKVDFNDDMEDLLDVKPDEWKGTAEEVAETCADALRITKKAEIDEYVQMFADAEGNM
                     VERAVVAFDQWLKG"
     gene            complement(2283..2813)
                     /locus_tag="VFC06_05705"
     CDS             complement(2283..2813)
                     /locus_tag="VFC06_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81418.1"
                     /translation="MVGSIGIETSAPTESSTAVPSVDPSASPGPTFAPEDHADDIASE
                     GDLAADQAAIDALCPEVAAWWKSVGSVSLDQWQKSGTGVYATSFVLDRYTEASYAALQ
                     RADWSQFDTMPITLPAAGYGACILYRPADASGQFETVILTFTALTPQPMPGTLDHQRS
                     VTLVAAELLAANLIAR"
     gene            3007..3585
                     /locus_tag="VFC06_05710"
     CDS             3007..3585
                     /locus_tag="VFC06_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81419.1"
                     /translation="MDKRYVSLGVVCGAVVGNVPVPLEVAIFVLNGDMQPLDKYSVPV
                     IPSDYYDLSMVSMHMPKHLSNALEQEGLWSEMQVNSLRVEAADRIIAEKIERHCELDK
                     FEVVVLCDRLEPTRDAMYRFYPTAAKKLDRRNASWLEVEPLKMFVETEKIYAGVPWGA
                     RAEVRAKMSAQYALQMRRTLSAGAKTLIAAQA"
     gene            complement(3593..>3885)
                     /locus_tag="VFC06_05715"
     CDS             complement(3593..>3885)
                     /locus_tag="VFC06_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81420.1"
                     /translation="VRGRERLAAIATPISSTPRREPGNEQQRSRIEPKGDGGGSKGYG
                     QHGGRTAQPRGNNQGKNSVAEAFGLALGGKRGEDGQAYSLYRLNDPDAESDG"
BASE COUNT          637 a         1280 c         1233 g          735 t
ORIGIN      
        1 gtagaagatg tcgtagacct cgtccagtgc agcgcgttgg agcatctgcg cgaagtgacg
       61 ctcgccgctg gccccgatct ccccctgctt gccgaacttc gtctgcgctt ggttcgagcc
      121 gagacccgcg ccagcccgac cgtgattgga ctgtggtagg cgcccgccgg gcaatggctg
      181 cttgtaggtc atcgaaccct ccgtttcccc ctgagggtaa cgcgtaaaag tcgggtcaaa
      241 tccgctctgg ccctcccgca acgtgggcga atgagcggtg gtgcccttgt cgcaccaggt
      301 tgtgctcggc gctactcgtt ccagtccggg cagaggtcgc gaattttgca gaacctgcac
      361 ggcgcatcgt ccttgctggc ggccggcgat gtaggcacgt agcccctgtc ggcgatcgcg
      421 gcggtctcgt tgccgatcat gtcgaggacc tcctcgacgt ctcgatacat ctccggcgtg
      481 gccttgatgg agaaccgctc ccccgacttg aggtacacca actggacccc ggccacagga
      541 agcgcgctga tgttgtccgc gcgcgccctg tggcgcgcca gagcatagat gccgctcggc
      601 aggaacgtct tgttgaatac cgacacctcg ccggtggggt tcttgccagt cttgtagtca
      661 acgatcttct cgagcccgtt gagggtgcgg tcggtgcggt cgatcttgcc gaacacattg
      721 aggcgcccct gctggagccg gacgatctgc tcgttggcca gtacgttcac ccgcggcggc
      781 gtctccatgt cgaagatcgc caacgcaagg tcgcggtagg tagtgcggaa gaacgacttg
      841 aacgacttcg gcgggtcggt gaggttcgcg agcacgctct cccactctgt cacgagcgac
      901 ttggaaacga tgccgtcgtc aatatcgccc gctttgaggc agtcccacgc gtgatcgtag
      961 atgtcttcca ggacctcccg tgtgcggaac ccgggcggct ctgtgtagag gacctccaag
     1021 gcgcggtgcg ccagcgagcc cccgacaccc cactggttcg cgggctgccg ggtgaagtcg
     1081 gtgatgccga ggatcgtctt gccggtcttc tcgtgcaacg gacccaggtg acgctccacg
     1141 aaccactttg tggggcattc gaggaacgaa gcgaaggagg acggtgattc cttctgcacc
     1201 ttcatgtccg cccaggcgtc caggagttcc gacggcaggt cgacaccctt gccgcctccg
     1261 ggcaccatgg cctcgactcg ctcggagtac ttggccttca cgtctgccat cgtggcgatc
     1321 agcctttgag ccactgatca aacgcaacca ctgcgcgctc gaccatgttg ccctcggcgt
     1381 ccgcgaacat ctggacgtac tcgtcgatct cggccttctt ggtgatgcgc agagcgtcag
     1441 cgcaggtctc cgcgacctct tcggccgtgc ccttccactc gtcgggcttg acgtcgagga
     1501 ggtcttccat gtcgtcgttg aagtcgacct tccccgactt gcaggcggtg cggatggtag
     1561 ccaccgtgac ctcggcggag tcgctcttgg ccgagtcgct cttggacgcg ccgcccgaac
     1621 tacgcgagga gccgcgcgag tcagagtcgt ggtcgcctcc attggagaag ttctcgcgga
     1681 tgctcgccag gtagcgaacg gtgtcgccgc cacccctgac agtcccctgc gtcttcgcga
     1741 atgccttgcg tacgaacacg tccttgccaa ccagcgcgag cgcgtcggct tcgttggcct
     1801 gagagagtgc catgtcgaag gcaaccttcg ggtcgcggag ccgctcgggg tcggtctcgc
     1861 ccatgaactc gacccagtgg gtcgacaact cctcgtcctt gtcgccgccg gagtccagaa
     1921 cgatgatggc cttcttgccc agcaggcgtc cgttggacga gcggtcgacg tgcttgactt
     1981 cgaggacctt ccccttgaag accttggagc ccgtcttctc gctgtcgggt acatggaccg
     2041 ccgcttggtg aacgagcgct cccgcgatga acccaatcac cgcggtcggg ctgacttcgg
     2101 acagttgtgc agcgaccacg ggcgggacga gcgcctcgta gccctcgacc ggatcgcgca
     2161 atgcgcgggc gacgtcaatg atgttagcca cgatgggccc ctctcgaaat tttccgacgg
     2221 tactatttgt gaatacctaa aggctaccac acctcaccga caagtgagcg ctgtggtagt
     2281 ccctaccgtg cgatgaggtt tgccgccagg agttcggcgg ccacgagcgt gaccgatcgc
     2341 tggtgatcta gcgtcccggg catgggctgc ggagtcagcg cggtgaacgt cagaatcacc
     2401 gtctcgaact gccccgaagc gtccgctggc cggtacagga tgcatgcccc gtacccggcc
     2461 gccggcaggg tgatgggcat ggtgtcgaac tgcgaccagt ccgcgcgctg cagcgccgcg
     2521 taggacgcct ccgtgtagcg gtccaggacg aacgacgtcg cgtacacgcc cgtgccggac
     2581 ttctgccact ggtccagcga gacagacccc acagacttcc accaggcggc tacctcgggg
     2641 catagggcgt cgatcgcggc ctggtccgcc gcaaggtcgc cctctgaggc gatgtcgtca
     2701 gcgtggtcct ctggcgcgaa cgtcgggccg ggactggctg acggatccac cgaggggacg
     2761 gctgtggacg attcggtcgg ggcggacgtc tcgattccga tgctgcccac cacggccgcg
     2821 gaaggacttg ccggcggcga atctccgccg tcactacccc cgcagcccgc cagagccatc
     2881 accgcgacga ataccactgg cacgagccga attcgctgag tcatcatgcc cccattctcg
     2941 caggaattgg ccccccattc tggtggtagc ctccaagcga cgcaggtgca cgaatggagg
     3001 tcggccatgg acaagcgata cgtcagtctt ggcgtggttt gtggcgccgt cgtgggcaac
     3061 gtgcctgtgc cgctcgaagt ggcgatcttc gtcctcaacg gcgacatgca gcccctcgac
     3121 aagtactcgg tcccggtgat cccatccgac tactacgacc tctcgatggt gtcgatgcac
     3181 atgccaaaac acctgtccaa cgctctcgag caagagggcc tgtggtcaga gatgcaggtc
     3241 aactcgctgc gcgtcgaggc cgcggaccgc atcattgcgg agaagatcga acgccactgc
     3301 gaactcgaca agttcgaggt cgtggtcctg tgcgaccgcc tggagccgac gcgcgacgcg
     3361 atgtaccgct tctaccccac tgccgccaag aagttggacc gccgcaacgc ctcatggctc
     3421 gaagtcgagc cgttgaagat gttcgtggag acagagaaga tctacgcggg tgtcccgtgg
     3481 ggcgcacgcg ccgaggtgcg cgccaagatg tcggcgcagt acgcgttgca gatgcgacgc
     3541 accctgtctg cgggggccaa gaccctcatc gccgcgcaag cgtgaattcg gcctagccgt
     3601 cgctctcggc gtcgggatcg ttcagcctgt acagagagta ggcctggccg tcctcgccac
     3661 gcttgccgcc cagtgcgagc ccgaaggcct ccgccacgct gttcttgccc tggttgttcc
     3721 ctcgcggctg ggcggtgcgt ccgccgtgct ggccgtaccc cttggatccg ccgccgtcgc
     3781 ccttgggctc aatacgcgac cgctgctgtt cgttccctgg ctcgcgccga ggcgtggacg
     3841 agatcggcgt ggcgatcgcc gcgagacgct cccgcccgcg aacga
//
LOCUS       DASRKH010000359         2382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_6901, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000359 DASRKH010000000
VERSION     DASRKH010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6901"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="VFC06_05720"
     CDS             <1..890
                     /locus_tag="VFC06_05720"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010524083.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HZL81421.1"
                     /translation="AAVHDAGGQVYIDGANLNALVGLAKPGHFGGDVSHLNLHKTFAI
                     PHGGGGPGVGPVAVADHLIEFLPAVRQSIQRPKDLRREGAAVSAAPYGSAGVLPIAWA
                     YIAMLGADGLRQSTQMAVLAANYVAVRLDEYFPVLFRGPGGLVAHECILDLRALTKAT
                     GVTAEDVAKRLIDYGIHAPTMSWPVAGTLMVEPTESEDLTELDRFIDAMISIRGEVDA
                     VANGEVSAEDSVLRHAPHTAEAVAADEWTAAYSREVAAFPVPSLRRDKYFPPVRRIDN
                     AYGDRNLMCTCPPVEEFEG"
     gene            937..2139
                     /gene="gcvT"
                     /locus_tag="VFC06_05725"
     CDS             937..2139
                     /gene="gcvT"
                     /locus_tag="VFC06_05725"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HZL81422.1"
                     /translation="MNRSPLHREHVALGATLTEFAGWEMPVRYAGDVAEHQAVRTTAG
                     LFDISHMGEIALRGPDAAAALDCAVVSGMARLAIGRAKYSMLCTPDGGIIDDLIVYRR
                     DWDHFIIVANASNVDTVTEELSGRCEPFDVAVENITHQVSLQAIQGPKAVDIVTEMCA
                     LGADDVRALKNYAWCTVLLSGDIHAMVARTGYTGEDGFELFVVPDDAVEVWRLALATG
                     TSHGLMPCGLSARDTLRLEAGMPLYGQELSLSTTPYHAGLGWVVQTGPARNVVRPGLD
                     GAVSLTGERVERGDFVGREALAIAKETHDAWAADPASAPDDARVLVALVGESRRAARP
                     GYAVMSGDAEVGVVTSGAPSPTLEKPIAMAMVHPSVSALGTELEVDVRGRREIMAVSA
                     LPFYTRAS"
     gene            2180..>2382
                     /locus_tag="VFC06_05730"
     CDS             2180..>2382
                     /locus_tag="VFC06_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012398578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HZL81423.1"
                     /translation="MPSIPADLLYSAEHEWVLGDVEPGSVVTVGITEHAANALGDVVF
                     VELPSVGDKVMAGEVCGELESSKA"
BASE COUNT          350 a          810 c          827 g          395 t
ORIGIN      
        1 gcgcggcggt gcacgatgcc ggcggccagg tctacatcga cggtgccaac ctcaacgcct
       61 tggtggggct cgcgaagccg ggccacttcg gcggcgacgt ctcccacctg aacctgcaca
      121 agaccttcgc gatcccgcac ggtggcggcg gtcccggcgt ggggccggtg gccgtggcgg
      181 atcacctcat cgagttcctg ccagccgtgc gccaatcgat ccagcgtccc aaggaccttc
      241 gacgcgaggg tgcggccgtg agtgcggcgc cctacggctc ggccggggtg ctccccatcg
      301 cgtgggcgta catcgccatg ctcggcgccg acggtctgcg gcagtcgacc cagatggccg
      361 tgctcgccgc caactacgtg gccgtgcgcc tcgacgagta cttccccgtg ctgtttcgtg
      421 ggcccggcgg cctggtcgcg cacgagtgca ttctcgacct gcgcgcgctg accaaggcga
      481 ccggagtcac cgccgaggat gtcgcgaagc ggctcatcga ctacgggatc cacgccccca
      541 cgatgtcctg gccggttgcc ggaacgctca tggtcgagcc gaccgagtcc gaggacctca
      601 ccgaactgga tcgcttcatc gacgccatga tctcgatccg tggtgaggtc gacgccgtcg
      661 cgaacggcga ggtctccgcc gaggactcgg tgcttcgaca cgcgccccac acggcggagg
      721 cggtggcggc cgacgagtgg accgccgcgt actcgcgtga ggtggcggca ttccccgtgc
      781 ccagcctgcg ccgcgacaag tacttccctc ccgttcggcg catcgacaac gcgtacgggg
      841 accgcaacct catgtgcacg tgcccgccgg tggaggagtt cgagggctga gtcgtccggg
      901 accacggcgt tccgctagga cgtctacgct gacgctatga accgctcccc actgcaccgc
      961 gagcacgtcg cgcttggcgc gaccctcacc gagtttgccg gctgggagat gcccgtccgc
     1021 tatgcgggtg atgtggcgga gcatcaggcc gtccgcacca ccgccgggct attcgacatc
     1081 agccacatgg gtgagattgc gttgcgcggt cccgatgccg ccgctgccct cgactgcgcc
     1141 gtcgtctcgg gcatggcgcg cctggccatc gggcgagcca agtactcgat gctgtgcact
     1201 ccggacggcg gaattatcga cgacctcatc gtgtaccgac gcgactggga tcacttcatc
     1261 atcgtcgcca acgcgtcgaa cgtggacacc gtgacggagg aactgtcggg tcgctgcgag
     1321 ccgttcgacg tcgcagtcga gaacatcacg caccaggtgt cgctgcaggc cattcagggc
     1381 cccaaggcgg tggacatcgt gaccgagatg tgcgcgctgg gcgccgacga cgtgcgcgcg
     1441 ctgaagaact acgcatggtg cacggtgctc ctgagcggcg acattcacgc gatggtggcg
     1501 cgcaccggct acaccggcga ggacggcttc gagctgttcg tcgtcccgga cgatgcggtc
     1561 gaggtgtggc gcctcgccct agccacgggc acgtcccacg ggctgatgcc gtgcggcctc
     1621 tcggcgcgcg acacgctccg actcgaggcg gggatgccgt tgtatggcca ggagctgtcc
     1681 ctgtcgacca cgccgtatca cgcgggtctt ggctgggtgg tgcaaaccgg gcccgcgcgc
     1741 aatgtggtgc gccctggact cgacggcgcc gtcagcctga cgggcgagcg tgtcgagcgc
     1801 ggcgacttcg tgggccgcga ggccttggcc atagccaaag agacccacga cgcgtgggcg
     1861 gcggaccccg catcggcgcc cgacgacgcg cgcgtgcttg tcgcactcgt gggcgagtca
     1921 cgccgtgcgg cgcgacccgg ctacgcggtc atgtcgggcg atgccgaggt gggcgtcgtc
     1981 acgtcgggcg ccccttctcc cacgttggag aagcccatcg cgatggcgat ggtgcaccct
     2041 tccgtgagcg ccctggggac tgagcttgag gttgatgtgc gcggtcgacg cgaaatcatg
     2101 gccgtgagcg cgctaccgtt ctacacacgg gcctcgtagc gaggcttggc ccgcattcct
     2161 gtgacgaaag gttgttgcca tgccctcgat tcctgcagac ctgctgtact ccgccgagca
     2221 cgagtgggtg ctaggtgacg tcgagcccgg ctcggtcgtg accgtcggca tcaccgagca
     2281 cgccgcgaac gcccttggcg acgtcgtgtt cgtcgagctg ccgtccgtcg gtgacaaggt
     2341 catggccggc gaggtgtgtg gcgagctcga gtcctccaag gc
//
LOCUS       DASRKH010000360         2608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_25768, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000360 DASRKH010000000
VERSION     DASRKH010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25768"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /gene="sufC"
                     /locus_tag="VFC06_05735"
     CDS             complement(<1..539)
                     /gene="sufC"
                     /locus_tag="VFC06_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012805067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HZL81424.1"
                     /translation="MSTLDIKNLHVTVDTPEGVKEILKGVDLTISSGETHAIMGPNGS
                     GKSTLAYSIAGHPKYTITEGSVTLDGADVLAMTVDERARAGLFLAMQYPVEVPGVSVS
                     NFLRTAKTAISGDAPKLRTWVKDVKTSMEALRMDPTFAERNVNEGFSGGEKKRHEILQ
                     MELLSPKIAILDETDSGLDV"
     gene            complement(536..895)
                     /locus_tag="VFC06_05740"
     CDS             complement(536..895)
                     /locus_tag="VFC06_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HZL81425.1"
                     /translation="MSEQFACNVGDLEPGSLVQSYLTAADGSEVPVAIARTEDGDFYA
                     INDICSHGEVSLAEGEMIGCEVECWAHGARFDVRNGAVTELPAINPVAAYPVRIDGER
                     VLVDVDNPLSSSIKENA"
     gene            complement(897..2093)
                     /gene="sufD"
                     /locus_tag="VFC06_05745"
     CDS             complement(897..2093)
                     /gene="sufD"
                     /locus_tag="VFC06_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HZL81426.1"
                     /translation="MSITTDHSKATADAEHTHKYNGTTVPDKSRADRLLSFDVDAFGM
                     PTGREENWRFSPMRDVAALLADEETGEHLDASYADLPQGVTITTITTDQFRARAVRAP
                     GDRASAIAAARSGGAMALAVAPGTTVEHPVRVVVTGTGQSVRQALSVEVGAGAEATVI
                     IERKGSAVVGEFISVDVGDNASLNLVLIQDWDIDTIHVGEVVAKLSRDARLRQSVVTL
                     GGKVVRLNTSVALAGEGASIDLYGLYFADAGQHQEHQLFVDHVAPRCRSRVTYKGALA
                     GATAHTVWVGDVLIRAAAEGTDTYELNRNLVLSDGARADSVPNLEIETGEIAGAGHAS
                     ATGRFDEEQMFYLRSRGITEDAARKLVVRGFFADLVRQIGVPEVEDGLMRAIEQELEQ
                     ITDEEN"
     gene            complement(2090..2473)
                     /locus_tag="VFC06_05750"
                     /pseudo
     CDS             complement(2090..2473)
                     /locus_tag="VFC06_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774983.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
     gene            2502..2608
                     /locus_tag="VFC06_05755"
                     /pseudo
     CDS             2502..2608
                     /locus_tag="VFC06_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003407484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          428 a          856 c          857 g          467 t
ORIGIN      
        1 acgtccaggc ctgagtccgt ctcgtccagg atcgcgatct tggggctcag cagttccatc
       61 tggagaatct cgtggcgctt cttctcgccg cccgagaagc cctcgttgac gttgcgctcc
      121 gcgaaggtgg ggtccatgcg cagcgcctcc atcgaggtct tgacgtcctt gacccaggtg
      181 cggagcttgg gagcatcgcc cgaaatcgcg gtcttggcgg tgcgcaggaa gttcgacacc
      241 gacacgccgg ggacctcgac cgggtactgc atcgcgagga acaggcccgc gcgtgcacgc
      301 tcgtccacgg tcatggcgag cacgtccgcg ccgtcaagcg tgacggagcc ctcggtgatc
      361 gtgtacttgg ggtgacccgc gatcgaatac gccagggtcg acttgccgga gccgttgggg
      421 cccatgatgg cgtgcgtctc gccggaagag atcgtcaggt caacgccctt gaggatctcc
      481 ttgacgccct cgggcgtgtc gacggtcacg tgcaggttct tgatgtccag ggtgctcatg
      541 cgttctcctt gatactggaa gaaagggggt tgtcgacgtc cacgagcaca cgctcgccgt
      601 cgatacgaac ggggtaggca gcgacgggat taatcgccgg aagttccgtg acggcgccgt
      661 tgcggacgtc gaatcgagcg ccgtgggccc agcactcgac ctcacagccg atcatttcgc
      721 cttctgccaa cgacacctcg ccgtggctgc agatgtcgtt gatcgcgtag aagtcgccgt
      781 cctcagtacg cgcgatcgcg acgggcacct cactgccgtc tgcggccgtg aggtaggact
      841 ggacgaggga tccgggctcc agatcgccca cgttgcaggc aaactgctcg ctcacgtcag
      901 ttctcctcgt ccgtgatttg ctcgagttcc tgctcgatgg ccctcatcag gccgtcttcg
      961 acctcgggca cgccaatctg ccggacgagg tccgcgaaga atccacgcac caccaacttg
     1021 cgggcagcat cctcggtaat accacgcgag cgcaggtaaa acatctgctc ttcgtcgaag
     1081 cggccggtgg ccgaggcgtg acctgcaccc gcgatttcgc cggtctcgat ttcgagattg
     1141 gggaccgagt ccgcacgcgc gccgtcggag agcacgaggt tgcggttgag ctcatacgtg
     1201 tcggtgccct cggctgcggc gcgaatcagc acgtcgccga cccacacggt gtgggccgtc
     1261 gcgccggcga gcgcgccctt gtacgtgacg cgcgagcggc aacgcggggc gacgtggtcg
     1321 acgaacaact ggtgctcctg gtgctgcccc gcatcggcga agtacaggcc atacaggtcg
     1381 atggaggcgc cctcgcccgc cagggcgacc gaggtgttga gtcgcacgac cttgccgcca
     1441 agcgtcacga cggactgtcg caagcgcgcg tcgcggctga gctttgcgac gacctcgccc
     1501 acgtgaatcg tgtcgatgtc ccaatcctgg atgagcacga ggttgaggga ggcgttgtcg
     1561 cccacgtcca ccgagatgaa ctcgccaaca acggccgagc ccttccgctc aatgatgacg
     1621 gtcgcctcgg ctcccgctcc cacttccacg gagagggcct gccgcacgga ctggccggtg
     1681 ccggtcacga cgacgcgtac ggggtgctcc acggtcgtgc cgggggcgac agccaacgcc
     1741 atcgctccac cggagcgcgc cgcggcaata gcactcgccc ggtcgcccgg cgcgcgcacg
     1801 gcacgtgcac ggaactggtc cgtggtgatg gtcgtgatcg tcacgccttg cggaagatcc
     1861 gcgtagctcg cgtcgaggtg ctcgccggtc tcctcgtccg cgaggagcgc ggcgacatcg
     1921 cgcatcggcg agaagcgcca gttctcctcg cgccccgtgg gcatgccgaa cgcatcgacg
     1981 tcgaaggaca ggagcctgtc cgcgcgcgac ttgtcgggca ccgtggtgcc gttgtacttg
     2041 tgcgtgtgtt cagcgtcggc cgttgccttg ctgtggtctg tggtgatgct caaccgacgg
     2101 atccttccat ctgcatttcg atgaggcgat tcagctcgag cgcgtactcc atgggcagtt
     2161 cgcgcgcgat gggttccacg aacccgcgca cgatcatggc catggcctcg gtctcgggca
     2221 tgcctcggga catcaggtag aacagctgct cctggcttac gcgggacact gtggcctcgt
     2281 ggcccatgga cacgtcgtcc tcgcgcacgt ccacgtacgg gtacgtgtcg gagcggctga
     2341 tctggtcgac caggagcgca tcacacagca cgttggactt ggagcccttc gcgccctcga
     2401 gaatctggac gagcccgcgg taggaggtgc gaccgccgcc gcgtgcgacc gacttcgaga
     2461 cgatcgacga cgagggctcc tacgtgcact acgtcgaggg ctgcaccgcg ccgatctaca
     2521 agtcggactc gctgcactcc gccgtggtcg agatcatcgt caagaagggc gcccgcgtgc
     2581 gctacacgac catccagaac tggtcgaa
//
LOCUS       DASRKH010000361         2627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_13315, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000361 DASRKH010000000
VERSION     DASRKH010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13315"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1511)
                     /locus_tag="VFC06_05760"
     CDS             complement(<1..1511)
                     /locus_tag="VFC06_05760"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZL81427.1"
                     /translation="MQATNPSFADFDIRPEIVAALAAAGITHPFPIQAMTLPVALAGH
                     DIIGQAKTGTGKTLGFGVPLLHRVVAPGEEGFDDLDAPGKPQALVVVPTRELAVQVAT
                     DLGTASTNRSVRIVQLYGGRAYEPQVSALTKGVEVVVGTPGRMIDLQNQGFLDLSHAR
                     CVVLDEADEMLDLGFLPDVEKLLSRTPASRTTMLFSATMPGAVVAMARRYMTQPTHIR
                     ANDPDDGGQTVKNIKQVVYRAHAMDKVEVLARILQSEGRGLTIVFARTKRTAAKVADD
                     LIERGFAAGAIHGDLGQGAREQALRAFRNGKVDVLVATDVAARGIDVDDVTHVINYQC
                     TEDEKIYLHRTGRTGRAGHKGTAVTFVDWDDMPRWGLINKALDLGIPEPVETYSSSPH
                     LYAELNIPVGTKGRLPRSKQTRAGLDAEVLEDLGETGKRHASAGGHAAGGRSSDHHTD
                     GGRGRGPRDGGSRDGGRARGARPAGDGSSSRNADAAPAAGATRSAEAPAGANGP"
     gene            1791..2471
                     /locus_tag="VFC06_05765"
     CDS             1791..2471
                     /locus_tag="VFC06_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like fold-containing protein"
                     /protein_id="HZL81428.1"
                     /translation="MTQTVQSGTSGTREDSIELLGLVAQLQLEAFTRSAEDSAVAPTL
                     EQRLALSRFAADAVGRRDRVMDRIVEVGGDPLAAMAHFDHVLDDFDLRTSPSTWWERL
                     LTSYVGYGVADDFCRIAAARLDESSRALVLDVLDDHSHADLAVAEIAAAGADDEVLVA
                     RLALWGRRLGGEALGVVQALLGSRPALLRIAAGTDVYIEAGEASGKLFGELTAEHTHR
                     MGRLGLTA"
     gene            complement(2520..>2627)
                     /locus_tag="VFC06_05770"
     CDS             complement(2520..>2627)
                     /locus_tag="VFC06_05770"
                     /inference="COORDINATES: protein motif:HMM:NF022749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3107 family protein"
                     /protein_id="HZL81429.1"
                     /translation="ELVDTRGRHIIVPTASIGYVEIGGETKGRVGFGTI"
BASE COUNT          337 a          894 c          994 g          402 t
ORIGIN      
        1 ggaccgttcg cgcctgccgg tgcctcggcc gagcgggtgg cgccggcagc cggagccgcg
       61 tcggcgttgc gggacgacga gccgtcaccg gccggtcgag caccgcgcgc acgtccgccg
      121 tcacgcgaac cgccgtcacg cggtccacgc cctcgtccgc cgtcggtgtg gtggtccgag
      181 gagcgaccgc cggcagcgtg gccgccggcc gacgcgtggc gcttgcccgt ctcgccgagg
      241 tcctcgagga cctcggcgtc gagaccggcg cgcgtctgct tggaccgcgg caggcgaccc
      301 ttcgtgccga ccgggatgtt gagctcggcg tacaggtgcg gggagctcga gtaggtctcg
      361 acgggctcgg ggatgccgag gtcgagcgcc ttgttgatca ggccccagcg cggcatgtcg
      421 tcccagtcga cgaacgtcac cgcggtgccc ttgtgccccg cgcgaccggt acggccggtg
      481 cggtgcaggt agatcttctc gtcctcggtg cactggtagt tgatgacgtg cgtgacgtcg
      541 tcgacgtcaa tcccgcgggc cgcgacgtcg gtcgcgacga ggacgtcgac cttgccgttg
      601 cggaacgcgc gcagcgcctg ctcacgtgca ccctggccga ggtcgccgtg gatcgcaccg
      661 gccgcgaagc cgcgctcgat caggtcgtcg gccaccttgg ccgcggtgcg cttggtgcgg
      721 gcgaacacga tcgtcaggcc gcggccctcg gactgcagga tgcgcgcgag gacctcgacc
      781 ttgtccatcg cgtgcgcgcg gtagacgacc tgcttgatgt tcttgacggt ctggccgccg
      841 tcgtccgggt cgttggcccg gatgtgcgtc ggctgggtca tgtagcggcg ggccatcgcg
      901 acgacggcac ccggcatggt cgccgagaac agcatcgtgg tccgcgacgc aggggtgcgt
      961 gacaggagct tctcgacgtc gggcaggaac ccgaggtcga gcatctcgtc ggcctcgtcg
     1021 agaacgacgc agcgcgcgtg cgacaggtcg aggaagccct ggttctgcag gtcgatcatg
     1081 cggccggggg tcccgacgac gacctcgaca cccttcgtga gcgcgctgac ctgcggctcg
     1141 tacgcgcggc cgccgtacag ctgcacgatg cgcacggacc ggttggtcga ggcggtcccg
     1201 aggtcggtcg cgacctggac ggcgagctcg cgcgtcggca cgacgacgag ggcctgcggc
     1261 ttgccgggcg cgtcgaggtc gtcgaagccc tcctcgccgg gcgcgacgac acggtgcagc
     1321 agcgggacgc cgaagccgag cgtcttgccg gtcccggtct tcgcctggcc gatgatgtcg
     1381 tgacccgcga gcgcgacggg aagcgtcatc gcctggatcg ggaacgggtg ggtgatgccg
     1441 gcggcggcga gcgccgcgac gatctccggg cggatgtcga agtccgcgaa ggacgggttg
     1501 gtggcctgca cgctcgctgc ggtgtcggcg tcggtggcct gtgcgctgtc cgtcgcaggg
     1561 tcgagggtgg tgtcggtcgt ggtcacagat gactcttcac tcgagtggtg gcgggccgca
     1621 cgcttcccgc ggcacgtggg ccgggtcggg agcgggcatc gtcacgcagg tggccggcag
     1681 ccgatcgtga atgtcattcg cgcggtgcgc gaggcggtcg agacgaccgg gcaaccgatg
     1741 tacgcaccca tgctaccgga cggtcctgcg ggcggcggta agatcatccg atgacccaga
     1801 cggtgcagtc aggcacgtcc ggcacgcgcg aggactcgat cgagctgctc ggactcgtcg
     1861 cccagctcca gctcgaggcg ttcacccgca gcgccgagga ttccgccgtc gcaccgaccc
     1921 tcgagcagcg cctcgcgctg agccggttcg ccgccgacgc ggtcgggcgc cgcgaccgcg
     1981 tcatggaccg gatcgtcgag gtcggcgggg acccgctggc tgcgatggcc cacttcgacc
     2041 acgtgctcga cgacttcgac ctgcggacct ccccgagcac gtggtgggag cggctgctca
     2101 cgtcgtacgt cggctacggc gtcgcggacg acttctgccg gatcgcggcc gcgcggctcg
     2161 acgagtcctc gcgcgcgctc gtcctcgacg tgctcgacga ccacagccac gcggacctcg
     2221 cggtggccga gatcgctgcg gcaggcgcgg acgacgaggt tctcgtcgcg cgtctcgcac
     2281 tgtggggccg acggctcggt ggcgaggcgc tcggcgtcgt ccaggcactg ctcggctcac
     2341 gtccggcgct gctgcggatc gccgcgggga ccgatgtcta catcgaggca ggggaggcgt
     2401 cgggcaagct gttcggcgag ctcaccgccg agcacacgca ccgcatgggc cggctgggcc
     2461 tgacggcctg acggcctgac ggctgaccgg ctcggccggc ggcacgcgtg cgcgggttgt
     2521 cagatcgtgc cgaagccgac ccgacccttg gtctcgccac cgatctcgac gtagccgatc
     2581 gatgcggtcg ggacgatgat gtggcgcccg cgcgtgtcga cgagctc
//
LOCUS       DASRKH010000362         2439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_24771, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000362 DASRKH010000000
VERSION     DASRKH010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24771"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="VFC06_05775"
     CDS             complement(<1..773)
                     /locus_tag="VFC06_05775"
                     /inference="COORDINATES: protein motif:HMM:NF024708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4081 domain-containing protein"
                     /protein_id="HZL81430.1"
                     /translation="MTDSLLRRGQYSLVPAQRRDLAEALDMCATDAVANVVPAMHLET
                     SSKAGVVPKGLWVVRRRGRGSRDLAGVLWCGANLTAVLPTASDEADDARAEVAAAIVA
                     RLSRPAAIVGDAELTLDLWGRIEAWWGPARQLRPSQVSMVIRRDPEPVATPRREVEPL
                     TRASMDDYAQLLPAAAHMFMGEVGYDPLQHGREAYEDRLMHLVRAGRSYIMYGDVHGK
                     RGVVFKAEVGVVGGGVAQIQGVWVQPSLRGLGYGSAGMA"
     gene            complement(770..1927)
                     /gene="ispG"
                     /locus_tag="VFC06_05780"
                     /gene_synonym="gcpE"
     CDS             complement(770..1927)
                     /gene="ispG"
                     /locus_tag="VFC06_05780"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HZL81431.1"
                     /translation="MSIPISLGMPEAPAPVLASRRPTRKIKVGKVDVGGDAPVSVQSM
                     CTTPTTDINATLQQIAALTAAGCDIVRVAVPTQDDAEALPAIARKSQIPVIADIHFQP
                     KYVFAAIDAGCAAVRVNPGNIRKFDDQIKEIARTAKDAGVAIRIGVNAGSLDPRLLAK
                     YGKATPEALVESAVWEASLFEEHDFHDFKISVKHNDPVVMVRAYEMLSERGDWPLHLG
                     VTEAGPAFQGTIKSATAFGALLSQGIGDTIRVSLSAPPVEEVKVGIQILQSLNLKERK
                     LEIVSCPSCGRAQVDVYELAEKVTAGLEGMEVPLRVAVMGCVVNGPGEAREADLGVAS
                     GNGKGQIFVKGVVIKTVAEKDIVETLIEEAMKLAATMEPVEGSAPQVVTAG"
     gene            complement(2040..2321)
                     /locus_tag="VFC06_05785"
                     /pseudo
     CDS             complement(2040..2321)
                     /locus_tag="VFC06_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015936625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
     gene            2316..2439
                     /locus_tag="VFC06_05790"
                     /pseudo
     CDS             2316..2439
                     /locus_tag="VFC06_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867337.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
BASE COUNT          358 a          887 c          804 g          390 t
ORIGIN      
        1 tgtgccatgc ccgcgcttcc gtaaccgagc ccacgcaggc tcggctggac ccacactccc
       61 tggatctgcg ccacgcctcc tcccaccacg cccacctcgg ccttgaagac gacgccacgc
      121 ttgccgtgga cgtccccgta catgatgtag ctgcgtccgg cgcgcacgag gtgcatcagc
      181 cggtcctcgt atgcctcgcg cccatgctgc agcgggtcgt agcccacctc gcccatgaac
      241 atgtgtgcgg cggcgggcaa cagctgcgcg tagtcgtcca tggacgccct tgtcagcggt
      301 tccacctcac gccgcggggt cgcgacgggt tcgggatccc ggcggatcac catcgagact
      361 tggctgggtc gcaattgccg cgccggaccc caccacgctt cgatcctgcc ccacaggtcc
      421 aacgtcagct cggcatcgcc cacgatcgcc gcaggcctgc tcagtcgcgc cacgatcgcc
      481 gcggcgacct cagccctggc atcgtccgcc tcgtcgctcg cggtgggcag caccgccgtc
      541 aggttcgccc cacaccacag cacgcctgcc aagtcccgcg acccacggcc gcggcggcgc
      601 accacccaca gccccttggg cacgactcct gcctttgagg aagtctcgag atgcatcgca
      661 ggcaccacgt ttgcgacggc gtcggttgcg cacatgtcga gcgcctccgc caggtcgcgg
      721 cgttgcgcgg gcaccagcga atactggcct ctacgcaaca gcgagtcggt catcccgccg
      781 taaccacctg cggcgcgctg ccctccacgg gctccatggt tgcggccaac ttcatggcct
      841 cttcgatcag cgtctccacg atgtccttct cggcgacggt cttgatgacc acgcccttca
      901 cgaagatctg gcccttgccg ttgcccgacg ccacgccgag gtcggcctcg cgcgcctcgc
      961 caggtccgtt cacgacgcat cccatgacgg cgacgcgcag cggaacctcc ataccctcga
     1021 ggcccgcggt gaccttctcg gccagctcgt acacgtccac ctgcgcgcgc ccacatgacg
     1081 ggcacgacac gatctcgagc ttgcgctcct tgaggttgag gctctggaga atctggattc
     1141 ctaccttgac ctcctcgacg ggaggcgccg acagcgacac gcggatggtg tcgccgatgc
     1201 cttggctcag cagagctccg aaggcggtcg cggacttgat ggtgccctgg aacgcggggc
     1261 ccgcctcggt gacgccgagg tgcagcggcc agtcgccgcg ctcgctcagc atctcgtagg
     1321 cccgcaccat caccacgggg tcgttgtgct tgaccgagat cttgaagtcg tggaagtcgt
     1381 gctcctcgaa gaggcttgcc tcccacacgg cggactcgac gagcgcctcg ggcgtggcct
     1441 tgccgtactt ggcgagcagc cgcgggtcga gcgaacccgc gttgacgccg atccggatcg
     1501 cgacgcccgc gtccttggcg gtgcgtgcga tctccttgat ctggtcgtcg aacttgcgga
     1561 tgttgcccgg gttgacgcgc acggctgcgc agccggcgtc gatcgccgcg aagacgtact
     1621 tcggctggaa gtggatgtcc gcgatcacgg ggatctgcga cttgcgcgcg atcgcgggca
     1681 gggcttcggc gtcgtcctgg gtggggacgg cgacgcgcac gatgtcgcag cccgccgcgg
     1741 tgagcgccgc gatctgctgg agcgtggcgt tgatgtcggt cgtcggcgtg gtgcacatcg
     1801 actggacgct cacgggcgcg tcaccgccga cgtcgacctt gccgaccttg atcttgcggg
     1861 tcgggcgccg agaggcaagc accggggctg gcgcctccgg catgccaaga ctgatcggga
     1921 tgctcacgcg tccattatcg ccactgtcgc cgcgcgcaac gccccgcggg gtgtgtctcg
     1981 cacgacatcg gcacatctcc cccgcggcgt ccctgcgcga acgcgcagcg gaggccgggt
     2041 cagccgaggg agaccgggtg gacgatgtcg gcgtagatca gcagcacccc gaggcccgcg
     2101 agcagcacga acaccccgta ggcgagcggg acgagccgtg cggtgtcgaa gggtcccggc
     2161 cgaggcaggc cgcgcacgcg cgcgacctga cgacgggctc cctcccacag ggcccccagc
     2221 acgtgcccgc cgtcgagcgg gagcagcggg atgaggttga acacgaacag cgcgatgttg
     2281 agcgaggcga cgaggctgag caggctcgcg acccgctcaa catcgcgctg ttcgtgttca
     2341 acctcatccc gctgctcccg ctcgacggcg ggcacgtgct gggggccctg tgggagggag
     2401 cccgtcgtca ggtcgcgcgc gtgcgcggcc tgcctcggc
//
LOCUS       DASRKH010000363         2286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_51268, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000363 DASRKH010000000
VERSION     DASRKH010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51268"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VFC06_05795"
     CDS             <1..807
                     /locus_tag="VFC06_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81432.1"
                     /translation="LNAVFRLWPVGKVDGDYRAEGLGRREETSPRALAWLAVSADRDV
                     RRGVASNPECPVSALRMFAEHGTVGEAASVADNPSSPTDALERVFARDSRSAFVSEDD
                     YYRFDIKESLGDNPSAPLWMVTLVLPRGDAIRRSGSEDRASTLVSDLHAAARTYAKRK
                     ALMCSVAANRSAPPELLHAISRYTRDAESISRLIANPATDVRTVASIEKSWDGLELPI
                     RVRVGEYLASVGVHPDNQGAVEFLATQHEWSVLTTESPEIALALALHPNP"
     gene            1290..>2286
                     /locus_tag="VFC06_05800"
     CDS             1290..>2286
                     /locus_tag="VFC06_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81433.1"
                     /translation="MAAALDVERLKARNWLRHGVPAARRYKVEAMLARDEVLDLGAYV
                     DGLRSADWLHDRMLATGVTITRLSADVHVDRSSVFEWLVTGVADSHRSQVEHAFEDGS
                     PATRAADAQRRADEYARFAALDLLAWLRARLAETGVQQAEVAAAMGLTEATMVSWNCH
                     GVPQHWEGKLRAALRRDAPLVSAARRRHATQGAYRWREAMKSAHQRRADVARALGVKP
                     ATVGLWLRQGVPDSRRAAFREFLDSAHLANAQERVSAAPPSALTGREVRAQQRAARAP
                     DSPLPRRYGPRTASALKPGHHNWLRERLFETGVHPRSVALALGVNTSVAQGWVNRI"
BASE COUNT          392 a          720 c          809 g          365 t
ORIGIN      
        1 ctcaacgcag tgtttcggct atggcccgtg ggaaaggtcg acggcgacta tcgcgcggag
       61 gggctcggtc ggcgggagga gaccagtccg cgggctctcg cctggctggc agtcagcgct
      121 gatcgtgatg tgcgacgtgg cgtcgccagt aacccggaat gccctgtgtc ggcgctacga
      181 atgtttgcgg aacacgggac tgtgggcgag gcggcgagcg ttgcggataa cccgtcctcg
      241 ccgacggacg cactcgaaag ggtgttcgcg agagattcgc gttccgcatt cgtctcggag
      301 gatgactact acagattcga catcaaggag tctctgggcg acaacccgtc cgctccgcta
      361 tggatggtga cgctcgtgct cccgcggggc gacgccattc ggaggtccgg ctctgaggac
      421 cgggcatcaa cgctggtcag cgatttgcac gccgcagcgc gcacctatgc gaagcggaag
      481 gcgctcatgt gctccgtcgc cgcgaaccga agcgccccgc cggaactact tcacgccata
      541 tcccgctaca cccgagacgc ggagagcatt tcccgactga tagccaatcc cgccaccgac
      601 gtgcgcaccg tcgcgagcat cgaaaagtcg tgggacggac tcgaacttcc gatcagggtt
      661 cgggtgggcg agtatctcgc gagcgtcggg gttcaccccg acaaccaggg cgcggtcgag
      721 tttctcgcca cccagcacga gtggtcggtc ctcaccaccg agtcgcccga gatcgctctc
      781 gccctagcgc tccacccgaa cccatgacag cggccgctcc cggagagaga atgacaggcg
      841 aagaactcgt tgcggagttg tggcagcgac atgcatctaa ggccgatctc gcccgcgttc
      901 tgtgtgtgag gacctcgact gtgcatatct ggtgcagtag tggcgtcccg cgcgtccggg
      961 tggcgcaggt tcgatcctgg atcgctccgg gctcgctact cccgaagggg gcagaaccag
     1021 cgccagtgcc cgagcatccc gcttggctta gcaacaggct gtccgagacg ggagtccgcc
     1081 agtgtgatgt cgcgtcagcg gtgggcgtgg ggcaggcagc agtgtcacgc tggaaggggt
     1141 ccgaggtgcc ggcgccgcgg ctggcggccc ttcgagatgt cctggccgac gattcgccgc
     1201 tggtgacggc ggagcgccgc aggcggagcg ccgaggctcg gctaaacagg gtgcttcgcc
     1261 aagcggcaga tcaatcgtca gtagcgaggg tggcggcggc gctggatgtt gagcgcctaa
     1321 aagcccggaa ttggctgaga cacggggttc ccgccgctcg acgctataag gtcgaggcga
     1381 tgctggccag ggacgaggtc ctcgatctgg gcgcatacgt ggacgggttg cgtagcgccg
     1441 actggcttca cgacaggatg ctcgccaccg gggtcaccat cactcggctc agcgcggatg
     1501 tccatgtcga tcgctcgtcg gtattcgagt ggttggtcac gggtgtggcc gacagtcacc
     1561 gaagccaagt ggagcacgca ttcgaagacg gctctccggc gacaagggcc gcggacgcac
     1621 agcgccgcgc ggatgagtat gcacgattcg cggcgctcga cctgctcgca tggctacggg
     1681 cgcgcctggc cgagaccggt gtgcaacagg cggaggtcgc ggcggctatg ggcttaacag
     1741 aggcgacgat ggtgtcgtgg aattgtcacg gcgtgccgca gcactgggag gggaaactgc
     1801 gcgctgcgct cagacgtgac gccccattgg taagcgccgc gcgccgccgc cacgccactc
     1861 agggcgccta ccggtggcgc gaggccatga agtccgcgca ccagagacgg gccgacgttg
     1921 cgcgggcgct cggcgtgaag cctgccactg tggggctgtg gctcaggcag ggcgtgccgg
     1981 actcgcggcg cgcagcgttc cgggagttct tggacagtgc acacctggcc aacgcgcagg
     2041 agcgtgtatc agcggcaccc ccgtccgcgc tgacgggtcg cgaagtcaga gcacaacagc
     2101 gcgccgcgcg cgcccccgac tcgccgctgc caaggaggta tgggccccgg accgcgtccg
     2161 cactcaagcc cgggcaccac aactggcttc gcgagcgact gttcgagacg ggcgttcacc
     2221 cgaggagtgt cgcgctcgcg ctaggcgtga acacgagtgt cgctcaaggg tgggtcaacc
     2281 gcatca
//
LOCUS       DASRKH010000364         3385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_3216, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000364 DASRKH010000000
VERSION     DASRKH010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3216"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VFC06_05805"
     CDS             <1..342
                     /locus_tag="VFC06_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZL81434.1"
                     /translation="VPETLELVGLAGKEKRMPHELSGGEQQRVAIARAVVNHPPILLC
                     DEPTGNLDPGTSIGIMRLLDRINRTGTTVLMATHDDEIVDQMRKRVIELKGGQLIRDQ
                     DRGVYGAEASV"
     gene            339..1250
                     /gene="ftsX"
                     /locus_tag="VFC06_05810"
     CDS             339..1250
                     /gene="ftsX"
                     /locus_tag="VFC06_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HZL81435.1"
                     /translation="MRLRFIFGEVFNGLRRNGTMALSVVLVTFVSLTFVGAAALTQMQ
                     VDKLKDDWYGKVEVSVFLCPEQSRHVQCAAGSATTAQEESIRSVLKDGSVAPLVQEVT
                     YESREEAFTKLRERDTDNRFALLTADGMQASFRVKLTDPEQFEVVADAVKGLPGVDEV
                     QDQREIFKGLFSLLNAATLIAAGLAAVMLLAAVLLITTTIRLSALSRRRETNIMRMVG
                     ASRGLIQMPFMLEGAVAATLGALMAATGLWLSVRYLVEDWLRGSVTFVDYISGADVLT
                     VAPWLLAIAVGLAAVASLATLGRYTRA"
     gene            1247..2566
                     /locus_tag="VFC06_05815"
     CDS             1247..2566
                     /locus_tag="VFC06_05815"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HZL81436.1"
                     /translation="MMTSPWRTRQFRLAAALLIAAILGASAGSIAQAEGSVPAWSSYD
                     DEIAQANKDKANKAEEAKNLEHDLSETSQAIADATIKLTQLNERLPVVQEEYRLAQQR
                     VDAAVLQQSIVVGKLEAARAQDAALTAQIDADDVKIDDLRSVLGEIARAEYQGSREDA
                     SLSIFFGAQTSTEFVDDYAYRETTSRVQSNTLAQMEELAAVNRNRKARQEAVRAYIEE
                     LKVKADALVVETQTARDAAAAKKAEVEQLLKEAEDLKAYLESQRAAYLAQLRQAEADA
                     AALKAELDVLWKKKLAEEATNGTGSLVKGFLSPPTAVPYITSSYGMRYHPIYHVWRLH
                     AGTDFRAYCGTPILAAADGKVEWARYVAGLGNQVMLYNGIVSGKVLYTSYNHLERFNV
                     SAGQTVRRGDVLGYEGTTGTSTACHLHFEVYVNGSTVDPMTLINKTW"
     gene            2654..3118
                     /gene="smpB"
                     /locus_tag="VFC06_05820"
     CDS             2654..3118
                     /gene="smpB"
                     /locus_tag="VFC06_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012877811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HZL81437.1"
                     /translation="MSDAVKVVATNRSARHDFFIEDVFEAGIVLTGTEVKSLREGRAS
                     IGEGYVSIIGGEAWCENIHIPEYTQGTWTNHAVKRKRKLLLHHTEIDELAIRTREKGH
                     TIVPLRLYFLKGRAKLEIGLAQGKKLYDKRQTLREKQDNREAERAMARRGRE"
     gene            3181..>3385
                     /locus_tag="VFC06_05825"
     CDS             3181..>3385
                     /locus_tag="VFC06_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81438.1"
                     /translation="MRTLGALLAAAFLVVVPAAAASATTNPDVPGREITRWDETFALN
                     VDGSADVRLEIDFDFGNDPGHGPY"
BASE COUNT          653 a         1040 c         1127 g          565 t
ORIGIN      
        1 gtgcccgaga cgctcgaact cgtgggcctc gcaggaaaag agaagcgcat gccccacgaa
       61 ctgtcgggcg gtgagcagca gcgtgtggcg atcgcccgcg ccgtagtgaa ccaccccccg
      121 atcctcctgt gcgatgagcc cacgggaaac ctcgaccccg gcacgtccat cggcatcatg
      181 cggctgctgg accgcattaa ccggaccggg acgacggtgc tcatggcgac gcacgacgac
      241 gagatcgtgg accagatgcg caagcgagtc atcgaactca agggcggtca gctcatccgc
      301 gaccaagatc gcggcgtgta cggggcggag gcctcggtat gagactccga ttcatctttg
      361 gcgaggtatt caacggcttg cgtcgcaacg gcacgatggc cctgtccgtg gtgctcgtga
      421 cattcgtgtc cctcaccttt gtgggcgccg cagcgctcac ccagatgcaa gtcgacaagc
      481 tcaaggacga ttggtacggc aaggtcgagg tctcggtctt cctgtgcccc gaacagtcgc
      541 gacacgtgca gtgcgccgcg ggcagtgcca ccaccgcgca ggaagagtcg attcggagcg
      601 tcctcaagga cggttcggtc gcgcccttgg tgcaagaggt cacctacgag tcacgcgagg
      661 aggccttcac gaagctgcgg gagcgcgaca cggacaaccg cttcgcgctc ctgacggctg
      721 atggcatgca ggcgagtttt cgggtgaagc tcaccgaccc cgagcaattc gaggtggtcg
      781 ccgatgccgt gaagggtctg ccgggcgttg acgaggtgca ggaccaacgg gagatcttca
      841 agggcttgtt cagtctgctt aatgccgcca ccttgatcgc cgcggggctg gcggccgtga
      901 tgctgctcgc cgcagtgctg ctcatcacga cgacgatccg cctgagtgca ctgagccgcc
      961 gtcgggagac gaacatcatg aggatggtcg gcgcctcccg cggcctgatc caaatgccgt
     1021 tcatgcttga aggtgcagtg gccgcgacgt tgggcgcgtt gatggctgcc acaggtctgt
     1081 ggctgtcagt gcgatacctc gtcgaagact ggctcagggg atcggtcaca tttgtcgatt
     1141 acatcagcgg ggctgacgtc ttgaccgtcg cgccgtggct cctggccatc gcggtggggt
     1201 tggcggccgt ggcatcgctc gcgactctgg gaaggtacac acgcgcatga tgacttcacc
     1261 gtggcgcacg cgccagttca ggcttgcggc agcgctcctc atcgcggcca tcctgggcgc
     1321 gagcgcgggc agcatcgcgc aggccgaagg ctccgtgccc gcttggtcca gttacgacga
     1381 cgagatcgcg caagcgaaca aggacaaggc caacaaggcg gaagaggcca agaacctcga
     1441 gcatgacttg agcgagacca gccaagcaat cgccgacgcc acgatcaagt tgactcaact
     1501 caacgagcgg ttgcccgtcg tgcaggagga gtaccggctc gcccagcagc gggtcgatgc
     1561 cgccgtgctt cagcagagca tcgtggtagg caagctcgag gccgccaggg cccaggatgc
     1621 ggctctgact gcccagatcg acgcggacga cgtcaagatc gatgacttgc gtagcgtcct
     1681 cggcgagatt gcacgcgccg agtatcaagg ctcgcgcgag gacgcgagcc tgagcatctt
     1741 ctttggcgcc caaacctcaa ccgagttcgt ggacgactac gcataccgcg agaccacgtc
     1801 acgcgtgcag tccaacacgt tggctcagat ggaggagctc gccgcggtca accgcaaccg
     1861 caaggctcgt caagaggcgg tgcgggcata catcgaggag ctcaaggtca aggccgacgc
     1921 cctcgtagtc gagacccaga ccgcgcgcga tgccgccgcg gcaaagaagg ccgaggtcga
     1981 gcagctgctc aaggaggccg aggacctgaa ggcctacctc gagtctcagc gcgcggcgta
     2041 cctggcccag ttgcggcagg ccgaggcgga cgccgccgcg ctcaaggccg agctcgacgt
     2101 cttgtggaag aagaagctgg ccgaagaggc gaccaacggc accggttcgt tggtgaaggg
     2161 cttcctcagc ccgcccacgg cagtgcccta catcacctcg agttacggga tgcgctatca
     2221 cccgatctat cacgtgtgga ggctccacgc ggggaccgac ttccgcgcat actgcggaac
     2281 acccatcttg gcggcggccg acggcaaggt cgagtgggct aggtatgtcg cgggcctcgg
     2341 caaccaggtc atgctctaca acggaatcgt cagcggcaag gtgctctaca cgagttacaa
     2401 ccaccttgag aggttcaacg tgtccgctgg gcagacggtg aggcgagggg atgtcctcgg
     2461 ttacgaggga accacgggta cgtccacggc ctgccacttg cacttcgagg tctacgtcaa
     2521 cggctccacg gtcgatccca tgaccctgat caacaagacg tggtaaggct gtcgcgtccg
     2581 cgttgagggc tctagactgg taggtcgcgc cgcaagagcg cgttcggacc ccaatgcgcc
     2641 cgggaggtga gccatgtccg atgcagtcaa ggtggtggcc accaacaggt cggcgcgcca
     2701 cgatttcttc attgaggacg ttttcgaggc gggcatcgtg ctgacgggca ccgaggtcaa
     2761 gtcgctgcgg gagggtcgcg cctccattgg cgagggctac gtgtccatca ttggtggcga
     2821 ggcgtggtgc gaaaacatcc acatccctga atacacgcag ggcacgtgga ctaatcacgc
     2881 ggtgaagcgc aagcgcaagt tgctgcttca ccacaccgag atcgacgaac tggccatccg
     2941 cacgcgcgaa aagggccaca cgatcgtgcc gttgcgcctg tatttcctga agggccgcgc
     3001 caagttggag atcgggctgg cgcaaggaaa gaagctgtac gacaagcggc agacgcttcg
     3061 ggagaagcaa gacaaccgcg aggcggagcg cgccatggcg cggcgcgggc gcgagtagtt
     3121 tctgcctcct aggccgcgcg gcccggggat gacgcgcccg gacacggcat gatgtgtttc
     3181 atgcgaactc ttggtgccct tcttgccgca gccttcctgg tggtggtgcc tgcagccgca
     3241 gcgtctgcga ccacgaaccc agatgtccct ggccgggaga tcacgcgttg ggatgagacc
     3301 tttgcgctca acgtcgacgg gtcggccgat gtgcggcttg agatcgattt tgactttggc
     3361 aacgatcccg gccacgggcc gtacc
//
LOCUS       DASRKH010000365         2386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_47001, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000365 DASRKH010000000
VERSION     DASRKH010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47001"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="VFC06_05830"
     CDS             <1..596
                     /locus_tag="VFC06_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HZL81439.1"
                     /translation="GASRRIHLHFLQAPHEVLGEDGKVTGVRVERTALTGDGSVKGTG
                     EFIDYPVQAVYRAVGYYGTPIDGIPFDASKGTIANVGGRVVDGGDVVPGYYTTGWIKR
                     GPVGLIGSTKSDAAETVGNLVEDADRLFAETEAGPEQLSPDNVLSLLKRRGVPVVEWH
                     DWEVLDAHERATGEADGRERLKVVPREDMTGIALRRD"
     gene            794..1672
                     /gene="htpX"
                     /locus_tag="VFC06_05835"
     CDS             794..1672
                     /gene="htpX"
                     /locus_tag="VFC06_05835"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012877286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HZL81440.1"
                     /translation="MTTRRYFNGAKTLGLFVLLWAVLLGLGSFISGGSYLWLFAAFGV
                     VSTFIGYWNSATLATRAMQARPVTEVEQPTMYRIVRELSQDANQPMPQLYVSPTAAPN
                     AFATGRNPSNAAVCCTEGILELLTERELRGVLGHELTHVYNRDILIGSVAAAIAGIVT
                     SVATMVMWFGGGRNSRDSPFGFVGLLAMLILAPLAATLIRMSISRTREFDADEDGAKL
                     TGDPAALASALRKLESGVAARPLRQTPQLENVSHMFIANPFKGVGLARMFSTHPPMDE
                     RIARLEEMNAHRGFLS"
     gene            complement(1692..>2386)
                     /locus_tag="VFC06_05840"
     CDS             complement(1692..>2386)
                     /locus_tag="VFC06_05840"
                     /inference="COORDINATES: protein motif:HMM:NF025615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YwiC-like family protein"
                     /protein_id="HZL81441.1"
                     /translation="IPPQHGVWAMLLLPFLAGAQLGFTWLHVSLLVVWLAGWLASYYA
                     LLAVKTLRPRRYWRQVLAYGVVSAVAALPLFALRPGLLWFAPVFAVLLAVNTVATRVG
                     QERASVNGVASVTMASLMAMIAPATAQLDWTIGIPVAIACWLYLAGTVFYVKNMIREH
                     GSRAHYVVSVAFHVGALAGAVAVNPWLALPFAWFLARAALLPRWHLKVPVVGAIEVVN
                     SLLLLGFLIALF"
BASE COUNT          424 a          820 c          791 g          351 t
ORIGIN      
        1 ccggcgcgtc gcgacgcatt cacttgcact tcttgcaggc gccccatgag gtcctagggg
       61 aggacggcaa agtgacgggc gtgcgcgtcg aacgcaccgc cctcacgggc gacgggagcg
      121 tcaagggcac gggcgagttc atcgactacc cggtgcaggc cgtgtatcgc gcggtcggct
      181 actacggcac gccgatcgac gggatcccct tcgacgcgtc caagggcacg atcgccaacg
      241 tcggcggccg tgttgtggac ggcggcgacg tggtgcccgg ttactacacg accggctgga
      301 tcaagcgcgg cccggtgggc ctcatcggct cgaccaagtc cgatgccgcc gagacggtcg
      361 ggaacctcgt cgaggacgcc gatcggctgt tcgccgaaac cgaggcaggc ccggaacagc
      421 tcagccccga caatgtgttg tcgctcctca agcgtcgtgg cgtgcccgtg gtcgagtggc
      481 acgactggga agtgctcgac gcccacgagc gggcgacggg cgaggccgac ggccgcgagc
      541 gcctcaaggt ggttccgcgc gaagacatga cgggcatcgc actgcgccgc gactagccct
      601 gggtccaccc ctccccgcca gtggctcaat ccgtaccgga tttcgcccgc acaccgccct
      661 cggtggctcg attcgtacca ccggcgcgga gccgacgccg gcgtgcgcgc cagccacgcc
      721 cacggcgaac tcgcctggcg tgctggaacg cgcaaagtgg gccgcccgtt cttaccagga
      781 cgaggaggaa cacgtgacga cccgacggta tttcaacggc gccaagacgc tgggactgtt
      841 tgtgctgcta tgggccgtgt tgttgggcct cggaagcttc atctccgggg gcagctacct
      901 gtggctgttc gcggccttcg gcgtcgtcag caccttcatc ggctattgga actcggccac
      961 gctcgcgacg cgcgccatgc aggcacgtcc cgtcaccgag gttgagcaac ccaccatgta
     1021 ccgcatcgtc cgtgaactct cgcaggacgc caaccagccg atgcctcagc tgtacgtgtc
     1081 gcccacagcg gcccccaacg cttttgcgac cggccgcaac ccctccaacg ccgccgtgtg
     1141 ctgcaccgag gggatcctgg aactgctgac tgagcgcgag ctgcgcgggg tgctcggcca
     1201 cgagctgacg cacgtgtaca accgcgacat cctcatcgga tccgtggccg cagcgatagc
     1261 gggaatcgtg acgtcggtcg cgaccatggt gatgtggttc gggggcggac gcaacagtcg
     1321 cgacagcccg tttggctttg tcggcttgtt ggcgatgctg attttggcac cgctggccgc
     1381 gaccctgatc cggatgtcga tttctcgcac gcgtgagttc gacgcggacg aggacggcgc
     1441 caagctcact ggggaccctg ccgccttggc gtcggcgctg cgcaaactcg aatccggcgt
     1501 cgccgcgcgc ccgctgcggc agaccccgca gctggagaac gtcagccaca tgttcatcgc
     1561 gaaccccttc aagggggtcg gcttggcgcg catgttctcc acgcacccac ccatggacga
     1621 gcgcatcgcc cgacttgagg aaatgaacgc gcatcggggc ttcctgagct agtccctgcg
     1681 ccatgcccgg tctagaacag ggcgatgagg aatccgagca gcagcaacga gttgaccacc
     1741 tcgatggcgc ccaccaccgg aaccttgagg tgccatctcg gcagcagtgc ggcacgcgcc
     1801 aggaaccagg cgaagggcag cgccagccag gggttcaccg cgacggcgcc cgcgagtgcg
     1861 cccacgtgga acgcgacgga gaccacgtag tgcgcgcgac tgccgtgctc gcgaatcatg
     1921 ttcttcacgt agaacacggt gccggcaagg tacagccagc acgcgattgc cacagggatg
     1981 ccaatggtcc agtcgagttg cgccgttgcc ggcgcgatca tcgccatcag cgaggccatc
     2041 gtcacggagg cgacgccgtt gacggacgcg cgctcctgac ccactcgggt cgccaccgtg
     2101 ttgacggcca ggagcaccgc aaagacgggc gcgaaccaca ggaggcccgg ccgcagcgcg
     2161 aagagcggaa gggccgccac ggcgctcacc accccgtaag cgaggacctg ccgccagtac
     2221 ctgcgcggtc gcagggtctt gacggccagc agcgcgtagt acgacgccaa ccatcccgcc
     2281 aaccacacga ccagcagcga cacgtgaagc caggtgaagc caagctgcgc gccggcgagg
     2341 aacggcagca acagcatggc ccacacgccg tgctgtggtg gaatcc
//
LOCUS       DASRKH010000366         2116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_15957, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000366 DASRKH010000000
VERSION     DASRKH010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15957"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VFC06_05845"
     CDS             complement(<1..401)
                     /locus_tag="VFC06_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HZL81442.1"
                     /translation="MVTGTHGPTANDHQSIGELIGRLSEQGARLVHAEIELAKAEMAA
                     RAQAAGIGLGLFAGAAMFGFFAVATLIATAIMGLANAVAPWLAGLIVSLALLILTATL
                     ALVGRNRLQAGTRTPDRTVENVKKDVDAVKEG"
     gene            complement(481..924)
                     /locus_tag="VFC06_05850"
     CDS             complement(481..924)
                     /locus_tag="VFC06_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HZL81443.1"
                     /translation="MNQTPDAERPRGSRFFDRFTEPFSDIARDKITQVEDKVRSTVKS
                     EVDAVGRSVKARAVEIRPSALAFGAAALLTFFGLALLLTAAVVGLAHAVSLWLSALII
                     GLVLVLGGAAFAALGRSRLPAPVHGTPPAPHPAPDADELVHPWAD"
     gene            1138..1311
                     /locus_tag="VFC06_05855"
     CDS             1138..1311
                     /locus_tag="VFC06_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HZL81444.1"
                     /translation="MGLDDKVANAAEHAKGTAKEAAGKATDNERLEAEGRLDQAKADV
                     KKVGEDVKDVLKD"
     gene            complement(1373..1445)
                     /locus_tag="VFC06_05860"
     tRNA            complement(1373..1445)
                     /locus_tag="VFC06_05860"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1410..1412),aa:Phe,seq:gaa)
     gene            complement(1485..1558)
                     /locus_tag="VFC06_05865"
     tRNA            complement(1485..1558)
                     /locus_tag="VFC06_05865"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1522..1524),aa:Asp,seq:gtc)
     gene            complement(1593..1665)
                     /locus_tag="VFC06_05870"
     tRNA            complement(1593..1665)
                     /locus_tag="VFC06_05870"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1629..1631),aa:Glu,seq:ttc)
     gene            1775..>2116
                     /locus_tag="VFC06_05875"
     CDS             1775..>2116
                     /locus_tag="VFC06_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HZL81445.1"
                     /translation="MSREDFEDAVRDALDEIPSELAAQMDNVVVLVEDDPPVGEPDLL
                     GVYEGFPLTERGEFWATGALPDRITIFRNPTLSMCDDRAEVVDEVAITVVHEIAHHFG
                     IGDDRLHELGWA"
BASE COUNT          343 a          712 c          755 g          306 t
ORIGIN      
        1 ccctccttga ccgcgtcgac gtccttcttg acgttctcga ccgtgcggtc cggcgtccgg
       61 gtacccgcct gcagcctgtt gcgcccgacg agcgccagcg tggcggtcag gatcaggaga
      121 gcgagcgaca cgatgagccc cgcgagccag ggcgcgaccg cattcgccag gcccatgatc
      181 gcggtcgcga tcagggtcgc gaccgcgaag aagccgaaca tggctgcgcc cgcgaacagg
      241 ccgagcccga tgcccgccgc ctgggcgcgt gccgccatct ccgccttcgc gagctcgatc
      301 tcggcgtgga cgaggcgagc gccctgctcg ctcagtcggc cgatgagctc accgatggac
      361 tggtggtcgt tcgctgtcgg gccgtgcgtc ccggtgacca tgtgccctca tctctcgtcc
      421 ggactccgct gtgcgttccg tgtgcccaca gtctggtgga caagccgacg acctgcgagg
      481 tcagtcggcc caggggtgga cgagctcgtc ggcgtcgggt gcggggtgcg gcgccggcgg
      541 cgtgccgtgg acgggcgccg ggaggcggct gcgccccagc gctgcgaacg ccgcaccgcc
      601 gaggacgagc acgagtccga tgatcagcgc ggacagccac aggctgacag cgtgagcgag
      661 gccgacgacg gccgcggtga gcagcagcgc gagaccgaag aacgtcagga gcgctgctgc
      721 gccgaacgcc agcgcgctcg ggcggatctc gacggcgcgg gccttgaccg accggccgac
      781 cgcgtcgacc tcggacttga cggtggagcg gaccttgtcc tcgacctgcg tgatcttgtc
      841 gcgcgcgatg tccgagaacg gctcggtgaa tctgtcgaag aagcgagagc cgcgcgggcg
      901 ctcagcatcg ggcgtctggt tcacgggtgg tcctccgtcg gatccgaggg tgcgcgcagg
      961 gcgcgcggtg tcgctcctgc cagcctaggt gcgccgcgcc cgtgaccggg gatgacatgc
     1021 caccgcggga cgatgttgct cacctcgtgg gatgccgcag ggccgctccc gacgcgcgca
     1081 ctcgcggcta tcgggttagc gtcgaacgat ctgatccgag cacaaggagg acgcaccatg
     1141 ggactcgacg acaaggtcgc caacgccgca gagcacgcca agggcaccgc caaggaggcg
     1201 gccggcaagg cgacggacaa cgagcggctc gaggccgagg gtcggctcga ccaggccaag
     1261 gccgacgtga agaaggtcgg cgaggacgtc aaggacgtgc tcaaggactg agcggcgtcg
     1321 gccaccaacg acgaagcccc gctccgatgt cttccgggcg gggcttctgg tgtggccact
     1381 ggcagggtcg aactgccgac ctttcgattt tcagtcgaac gctctaccaa ctgagctaag
     1441 tggccgaacc gacaacgccc ggccagatga ccgggcgttt cgagcgaccc cgacgggact
     1501 tgaacccgcg acctccgccg tgacagggcg gcgcgctaac cagctgcgct acggggcctt
     1561 gtggtgagac aaggtacctg acgtactgct cgtaccccca acgggattcg aacccgtgct
     1621 accgccgtga aagggcgggg tcctgggccg ctagacgatg ggggctcgac tgcccccgac
     1681 gtcgaggcgt cttcagaccg gagtcgagca tacggtctgg cggcccgaac tccaaagtcg
     1741 gggccgtgag ggccatgatg gacgcgtggt cgacatgtcg cgcgaggact tcgaggacgc
     1801 cgtccgggac gccctcgacg agatcccgtc cgagctggct gcgcagatgg acaacgtcgt
     1861 ggtgctcgtc gaggacgacc cacccgtggg cgagcccgac ctcctcggtg tctacgaggg
     1921 tttcccgctc accgagcgcg gtgagttctg ggcgaccggg gccctcccgg accgcatcac
     1981 gatcttccgc aacccgacgc tctcgatgtg cgacgaccgc gccgaggtgg tcgatgaggt
     2041 cgcgatcacg gtcgtccacg agatcgcgca ccacttcggc atcggcgacg atcgacttca
     2101 cgagctcggc tgggcc
//
LOCUS       DASRKH010000367         3031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_49185, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000367 DASRKH010000000
VERSION     DASRKH010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49185"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VFC06_05880"
     CDS             complement(<1..644)
                     /locus_tag="VFC06_05880"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HZL81446.1"
                     /translation="MPAPVGVLCVDCVRAANQAARGARSGLGFPRALGRPYVTIGIIA
                     ANVAFYVYGMGAGLYGWQTTFGLSPAASGQEPWRWITSGFVHGGLFHIGMNMFVLYQF
                     GSQLEQVMGRLRYGLLYGISLLGGSAAIQLLAAPNSLHIGASGAIFGLFGAYGLLLYK
                     LKLPWQSMAATAGIWLAVGFFLPNISWEGHIGGLVLGTLTMLAMMRLASRTPLRR"
     gene            complement(772..1311)
                     /locus_tag="VFC06_05885"
     CDS             complement(772..1311)
                     /locus_tag="VFC06_05885"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZL81447.1"
                     /translation="MIATLQTTAGDIRIELFPNHAPVTVANFTGLASGSKEWKDPMTG
                     ETRAEPFYDGLVFHRVIEGFMIQGGCPLGTGTGGPGYNFDDEIHPELAFNEPYLLAMA
                     NAGKRLNPITGKPGGTNGSQFFITVGPTPHLTGAHTIFGKVMDDESRAVVDKIATAPV
                     GAGDRPVDDVMVTGVSIEE"
     gene            1453..2640
                     /locus_tag="VFC06_05890"
     CDS             1453..2640
                     /locus_tag="VFC06_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81448.1"
                     /translation="MQRRWDEIDVDKYRKQAFDAAESALEAAERAADAASKAARTAKE
                     WASPRADAAGKAARTAKKWAGPRADRAKDWAGPRVEEAKEWATPRAEKAARTAARKAS
                     PYVHKAGDAAGHWVDVARGALVGAAIPAVVSAIDRAGEEETDKRGGGTWAKVLIPVII
                     AGAAGAALVVWARRDRGRDDWAGDDDWEFAGEEGDFQAKLRHDVNRAADSAAAAAKKA
                     AAAVAAAAGTVGEKAGPAYGRAKEATVTEAKKLVDQSQPYIDKAKDAAAPYVDKAVET
                     ASAAATKARDVAVPVLDKALEQATPYVSKARETAAAEAKKLADQGQPYADKARTTAAA
                     EAKRVREGVTTARSRAASGIANTLDDAEDVWEDDADDEAPAKKAPAKRAPVKKAPVKK
                     PTE"
     gene            2785..>3031
                     /locus_tag="VFC06_05895"
     CDS             2785..>3031
                     /locus_tag="VFC06_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81449.1"
                     /translation="MSTVAVAVDAFANRPDNAPGDPGSIPANQEVSEVKKLINSPETV
                     LADSLAGIAAAHPELRVDTVNRIIFRATPKSAGKVALL"
BASE COUNT          563 a          985 c         1031 g          452 t
ORIGIN      
        1 cggcgaaggg gcgtcctgct cgccaggcgc atcatcgcca gcatcgtcag cgtgccgagc
       61 acgaggccgc cgatgtggcc ctcccacgag atattcggca ggaaaaagcc cacggccagc
      121 cagattcccg cagtcgcggc catgctctgc cacggcaact tgagcttgta cagcagcagt
      181 ccatacgcgc caaacaggcc gaagatcgcg cccgatgcgc cgatatggag cgagttgggc
      241 gccgccagca attggatcgc tgcggatccg ccgaggagcg atattccgta gagaagcccg
      301 tacctcaggc ggcccatgac ttgctccagt tggctaccaa actggtagag cacaaacatg
      361 ttcatgccga tgtggaatag gccgccgtgg acaaagcccg acgtgatcca ccgccacggc
      421 tcttggccag acgccgcggg cgataggcca aaggtggttt gccagccgta cagccccgcg
      481 cccatgccgt acacgtagaa cgcgacgttc gcggcgatga tgccgatggt gacgtaggga
      541 cgcccgaggg cgcgcggaaa gccgagtccc gagcgggcgc cacgcgccgc ctggttcgcg
      601 gcacgcacgc agtcgacgca cagcacacct acgggcgcgg gcacctggca ctgcgggcag
      661 acggggcgct cgcaccgctg acaccgcacg taactcacac gatcggggtg ccgggggcac
      721 actgggggtg cctgaccggc cgccccctgc gtcggctcgc tcacgccgtg gctactcttc
      781 gatcgagacg cccgtgacca tcacgtcgtc tacgggacgg tcgcccgcgc caaccggcgc
      841 ggtggcgatc ttgtcgacta cggccctcga ctcgtcgtcc atgaccttgc caaagatggt
      901 gtgcgcgccc gtcaggtgcg gggtcggtcc cacggtgatg aagaactgcg aaccgttggt
      961 gccgcccggc ttgccggtga tggggttgag tcgcttgccc gcgttagcca tggcgagcag
     1021 gtagggctcg ttgaaggcca gctccgggtg aatctcgtcg tcaaagttgt agccgggccc
     1081 gccggtgccg gtgccgagcg ggcaaccgcc ctggatcatg aagccctcga tgacgcggtg
     1141 gaacacgaga ccgtcgtaga acggttcggc gcgcgtttca cccgtcatgg ggtccttcca
     1201 ctccttggag ccggaagcga ggcccgtgaa gttagcgacg gtcacggggg catggttcgg
     1261 gaacagttcg atgcgaatgt cacccgcggt ggtctgcagt gttgcgatca ttcggccatt
     1321 gtgtcacgcg cgtcgacggg cacttgggct ggttcggctc tgttcgctgg tggcgcaggg
     1381 aacaaagggg cctcgcgcat cgctgtccca agtggcagga tggtaggcag aaccacgacc
     1441 tgggaggccg caatgcaacg acgttgggat gaaatcgacg tggacaagta ccgcaagcaa
     1501 gcttttgacg cggccgaatc cgcgctcgag gccgccgaac gcgcagccga tgccgccagc
     1561 aaggccgccc ggaccgcgaa ggaatgggct tctccgcgcg ccgatgccgc cgggaaggct
     1621 gcacggactg cgaagaaatg ggccgggcca cgcgccgatc gcgcgaagga ctgggctggt
     1681 ccgcgcgtgg aagaagcgaa agagtgggcc acgccgcgcg ccgaaaaggc tgcgcgcact
     1741 gccgctcgca aggcgagccc ctacgttcac aaggctggcg acgccgccgg gcactgggtc
     1801 gacgtggctc gtggagcgct cgtgggcgcc gccattccgg cggtcgtctc ggcgatcgac
     1861 cgcgcaggcg aggaagagac cgacaagcgt ggcggtggga cgtgggccaa ggtgctcatt
     1921 cccgtcatca tcgcgggcgc cgctggtgcg gcgctagtgg tctgggcgcg tcgcgaccgt
     1981 ggtcgcgacg actgggctgg tgacgacgac tgggagttcg ccggagaaga gggtgacttc
     2041 caggcaaagt tgcgccacga cgtgaaccgc gcggcagatt cggccgcggc ggccgccaag
     2101 aaggcggccg ccgccgtggc cgctgccgct ggcaccgtcg gagagaaggc cggtcccgcc
     2161 tacggaagag ccaaggaggc cacggtgacc gaagccaaga agctcgtcga ccagagccag
     2221 ccttacatcg acaaggccaa ggacgccgct gcgccctatg tggacaaggc cgtcgaaacg
     2281 gcatccgccg ccgcgacgaa ggcccgtgat gtcgctgttc ctgtgctcga caaggccctg
     2341 gagcaggcca cgccatatgt ctccaaggcc cgcgagactg ctgccgccga ggccaagaag
     2401 ctcgcggacc agggccagcc ctacgccgac aaggcgcgga ccaccgcggc agcggaagcc
     2461 aagcgtgtac gcgaaggcgt caccaccgca cggtcccgtg ccgcctccgg tatcgccaac
     2521 accctcgacg atgccgagga cgtttgggag gacgacgcgg acgacgaggc tcccgccaag
     2581 aaggctcccg cgaagcgggc tccggtcaag aaggctccgg tcaagaagcc gaccgaatag
     2641 cacgtcgcga ctccgggtcg cacaggaagg gccgcgccat tggtgcggcc cttctctttt
     2701 tcccgtagtg tatgtgtgta cgaacacaca ttcgcgctca gtccgcttga agacggagtg
     2761 ttccactctg ttgaaccggg catcgtgagc accgtcgccg tcgccgtcga cgctttcgcg
     2821 aatcgccccg acaatgcgcc aggcgatcct ggtagcatcc cggccaatca ggaggtctcg
     2881 gaagtgaaaa agctcatcaa ttccccggag acggttctcg cggattcgct cgcaggcatc
     2941 gcggccgcgc acccggaact tcgcgtcgat acagtaaatc ggatcatctt tcgagcgacc
     3001 cccaagtcag caggcaaagt cgccttgctt t
//
LOCUS       DASRKH010000368         2239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_45004, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000368 DASRKH010000000
VERSION     DASRKH010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45004"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..1510
                     /gene="purD"
                     /locus_tag="VFC06_05900"
     CDS             227..1510
                     /gene="purD"
                     /locus_tag="VFC06_05900"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HZL81450.1"
                     /translation="MKILLLGSGGREHALARALAADPVTDELLVAPGNPGTAAIATNV
                     ALDINDPGAVAGFAASRKVDLVVVGPEAPLVAGVADAVAGVGIAVFGPSAEAARLEGS
                     KAFAKEIMAAAGVPTAMARVCTTLDEVRDAMDSFGAPHVIKADGLAAGKGVVVTSDAA
                     EALAHAATCLVRTGEDEPRVVVEDYLDGPEVSLFCVCDGVDVVPLAPAQDFKRALDGD
                     AGPNTGGMGAYSPLPWAGEDLTDEVLRRVARPVLREMAGRGTPFTGVLYCGLALTSRG
                     LRVVEFNVRFGDPETQVVVPRLRTPLAGLLYSAARGRLSTADEPRWSRGAAVTVVVAA
                     EGYPGTVRSGDPVTGIEEAEALEGVHVIHAGTRRDDDGALVSSGGRVLCVVGQGESLD
                     AARARAYEGVARIRLAGSHHRGDIAANVDSVSIPS"
     gene            1562..>2239
                     /locus_tag="VFC06_05905"
     CDS             1562..>2239
                     /locus_tag="VFC06_05905"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010143082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HZL81451.1"
                     /translation="MFDLDGWAHVSSGKVRDLYAPQDASAHGSGDVILVVSSDRISAF
                     DHVLPTPIPDKGKILSQLSLWWFDQLKDVIPNHVVSLDVPAAVRGRAMICHRLNMLEV
                     ECVARGYISGSGLSEYRATGAICGIELPAGLREGDELAEPIFTPAAKAHLGEHDENIS
                     FEELKRRVRAFAPMLRATTLELYSRAREIAARQGLILADTKFEFGTAAESSFARDIIL
                     ADEVLTSD"
BASE COUNT          312 a          802 c          799 g          326 t
ORIGIN      
        1 gaccaaggag tacttcgacc agttcggtga caaggtgccc gccgcgctct acgccgaact
       61 cgacgcgctc cgcgcccgga tcgacgccgc ccgcgaggga tagccgaccc gccaaacaga
      121 cggatgcccg gcacacccca cggtgtgccg ggcatccggg cgcttgcggt cccacaggct
      181 gtcatggccg cgatcaatcc gtgcgccgca cgttaggctc acgcttgtga aaatcctcct
      241 cctcggatcc ggtggccgcg agcatgcgct cgcgcgggcg ctcgccgctg atccggtgac
      301 cgacgagctg ctcgtcgccc ccggcaaccc cggcaccgcc gcgatcgcca ccaacgtggc
      361 gctggacatc aacgatcccg gggcggtggc cgggttcgcg gcgtcgcgca aggtggacct
      421 cgtcgtcgtc gggccggagg cgcccctcgt ggccggggtc gcggacgccg tggccggagt
      481 cggcatcgcc gtgttcgggc cgagcgccga ggccgcgcgg ctggagggtt cgaaggcctt
      541 cgcgaaggag atcatggccg ccgccggggt tcccacggcg atggcccgcg tctgcaccac
      601 cctcgacgag gtgcgggacg cgatggacag cttcggggcg ccccacgtga tcaaggcgga
      661 cgggctggct gccggaaagg gcgtcgtcgt gacgtccgac gccgccgagg cgctcgccca
      721 cgcggccacc tgcctcgtcc gcacgggcga ggacgagccg cgcgtcgtcg tcgaggacta
      781 cctggacggc cccgaggtct cgctgttctg cgtgtgcgac ggcgttgacg tggtgcccct
      841 cgccccggca caggacttca agcgcgccct cgacggcgac gccggcccca acaccggcgg
      901 catgggcgcc tactccccgc tcccgtgggc gggggaggac ctcacggacg aggtgctgcg
      961 gcgcgtcgcc cgccccgtgc tgcgcgagat ggcggggcgc ggcaccccat tcaccggcgt
     1021 cctgtactgc ggtcttgccc tgacctcccg cgggctgcgg gtggtggagt tcaacgtccg
     1081 cttcggtgac ccggagaccc aggtggtggt cccgcggctg cggaccccgc tggccggcct
     1141 cctgtacagc gccgcccggg ggcgcctctc caccgccgac gagccgcggt ggtcccgcgg
     1201 tgccgccgtc accgtggtgg tcgcggccga gggctatccc ggcacggttc gctccggcga
     1261 cccggtcacc ggcatcgagg aggccgaggc actcgagggc gtccacgtga tccacgcggg
     1321 gacgcggcgg gacgacgacg gcgccctggt gagttccggg gggcgcgtgc tgtgcgtcgt
     1381 cgggcagggg gagagcctcg acgcggcgag ggcgcgagcc tacgagggcg ttgcgaggat
     1441 ccgcctcgcg ggctcccacc accgcggcga catcgccgcc aatgtggatt cggtctcaat
     1501 accgtcatga acgggcaaac ccgcatgaaa caatgcattc ttgggggaga gggaggggtg
     1561 catgtttgat ctggatggat gggcgcacgt gtcgtcgggc aaggtccgcg acctctacgc
     1621 gccccaggat gcgtccgcgc acggcagtgg cgacgtcatc ctcgtcgtgt cctccgaccg
     1681 catctccgca ttcgaccacg tcctgcccac gcccatcccg gacaagggca agatcctcag
     1741 ccagctcagc ctgtggtggt tcgaccagct gaaggacgtc atccccaacc acgtcgtgag
     1801 cctcgacgtg ccggcggcgg tgcgcgggcg ggcaatgatc tgccaccggc tcaacatgct
     1861 cgaggtggaa tgtgtggccc gcggctacat ctccggctcg ggactgagcg agtaccgggc
     1921 caccggcgcc atctgcggta tcgagctccc ggccggtctg cgtgaggggg acgagctcgc
     1981 ggagcccatc ttcacccccg ctgccaaggc gcacctcggc gagcacgacg agaacatctc
     2041 gttcgaggag ctgaagaggc gggtgcgtgc gttcgccccc atgttgcggg ccaccacgct
     2101 ggagctctat tcgcgggcgc gggagatcgc cgcccggcag ggcctcatcc tcgcggacac
     2161 caagttcgag ttcggcaccg ctgccgagtc ctccttcgcg cgggacatca tcctcgccga
     2221 cgaggtgctc acctcggac
//
LOCUS       DASRKH010000369         2214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_60044, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000369 DASRKH010000000
VERSION     DASRKH010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60044"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1215
                     /locus_tag="VFC06_05910"
     CDS             <1..1215
                     /locus_tag="VFC06_05910"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HZL81452.1"
                     /translation="SGADIEALLDAREGAVLPARTRHLGFGDVAAVWTVLERLDVSGV
                     IDSVVGARRSDAGASVGRYLALAALNRVVAPTSKLQFEHWWKTTAADRFTKIPAGALD
                     HRRFWDAMHAITLEQLAEVEQQLAVRMIETFGLDISALALDMTNFATYIDTGNEKAPI
                     AQRGKAKQKRADLRLIGLGLVVTRDGGVPLVSHAYPGNRPDVTQFATMIDLLTARHGA
                     LADQTGAPTPDQMTVVFDAGQNSVENFEHLTGSGLAFVGSIPPSDCADLLALPATDRT
                     LVDADRFGALSALETHRVVYGTNRRVILTHSPTLHSAQQVGFAQTLAKALKALSDLAD
                     TLARGQGQRRDRRDHARPVATPRPALPAQRRHPGRAPAHRGRRRKSPRRPGRRGLRQT
                     DPDHQPGTLAGR"
     gene            1121..1579
                     /locus_tag="VFC06_05915"
     CDS             1121..1579
                     /locus_tag="VFC06_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81453.1"
                     /translation="MAVDEKARDALEDEVFGKRILITNQEHWPVAEVIAAYRSQSEVE
                     FSFRQLKDPRVVSFSPMHHWTEHNIRVHTFTCVLALQIAHLMRREAARAKLNLSVREL
                     LDQLVGIEETVLIYPSTGGRPKARRILTETTQTQDQLSEIFNLTKWAPRT"
     gene            complement(1645..1812)
                     /locus_tag="VFC06_05920"
     CDS             complement(1645..1812)
                     /locus_tag="VFC06_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81454.1"
                     /translation="MLTATKTTCTCIPEVPWECVSCYTARRDREQLLKSARNILKGIR
                     IENRCHLAGLS"
BASE COUNT          400 a          796 c          683 g          335 t
ORIGIN      
        1 tcgggcgccg acatcgaggc tctgctggat gcccgggagg gcgcggtgct tccggcgcgg
       61 acccgccatc tgggcttcgg tgacgtcgcg gcggtgtgga cggtgctgga gcgcctggac
      121 gtgtccgggg tgatcgactc agtggtcggg gcccggcgct ccgacgcagg cgcctcggtc
      181 gggcgctacc tggcgttggc cgcgctgaac cgggtggtcg cgcccacctc gaaacttcag
      241 ttcgagcact ggtggaaaac caccgcggcg gaccggttca ccaagatccc ggcgggggcc
      301 ctggaccatc gccggttctg ggacgcgatg cacgcgatca ccttggagca gctcgccgag
      361 gtcgagcagc agctggcggt acgcatgatc gagaccttcg ggctggacat ctccgcgttg
      421 gcgttggaca tgaccaactt cgccacctac atcgacaccg gcaacgagaa ggcaccgatc
      481 gcccagcgcg gcaaggccaa gcagaaacgt gccgacctgc gcctgatcgg actcggcctg
      541 gtcgtcaccc gcgacggcgg cgtccctttg gtctcccacg cctaccccgg caaccgcccc
      601 gacgtcaccc agttcgccac catgatcgac ctgctcaccg cccggcacgg cgctctggcc
      661 gaccagaccg gcgccccgac accggaccag atgacggtgg tctttgacgc cgggcagaac
      721 tcggtggaga acttcgagca cctgaccggg tccgggctgg cgttcgtcgg ttcgatccca
      781 ccatcggact gcgccgacct gctcgcccta cccgccaccg accgcaccct ggtcgacgcc
      841 gaccggttcg gggccctgag cgcgctcgag acccaccggg tcgtctacgg caccaaccgg
      901 cgggtgatcc tgacccactc accgaccctg cacagcgccc agcaggtcgg gttcgcccag
      961 accctggcca aggccctcaa agcgctcagc gacctggccg acaccctggc ccgcggccaa
     1021 ggtcaacgcc gcgatcgccg cgatcacgca cgacccgtgg ctacgccgcg tcctgcactg
     1081 ccagctcagc ggcgccaccc cggccgagca ccggctcacc gtggccgtcg acgaaaaagc
     1141 ccgcgacgcc ctggaagacg aggtcttcgg caaacggatc ctgatcacca accaggaaca
     1201 ctggccggtc gctgaggtga tcgccgccta ccgctcccag tccgaagtcg agttcagctt
     1261 ccgccaactc aaagaccccc gcgtcgtctc cttctccccc atgcaccact ggaccgaaca
     1321 caacatccgg gtgcacacct tcacctgcgt gctcgcccta cagatcgccc acctgatgcg
     1381 ccgcgaagcc gcccgagcca aactgaacct ctcggtccgt gaactcctcg accagctcgt
     1441 cggcatcgaa gagaccgtcc tgatctaccc ctcgaccggc ggccgtccca aggcccgacg
     1501 gatactcacc gaaaccaccc aaacccaaga ccagctcagc gagatcttca acctcacgaa
     1561 gtgggctccc cgaacttagg tcatacgcca cttgaagaac aacatggccc tgacctgcac
     1621 gtttacaaga ctggcccgag ctaactagga aagtcccgct aggtggcaac ggttctcgat
     1681 gcggatcccc ttgaggatgt tgcgggcgga cttgagaagt tgctcgcgat cacgtcgcgc
     1741 ggtgtagcag gagacgcact cccaagggac ttcggggatg caggtgcagg tggtcttggt
     1801 cgcggtgagc attgtgttgc ctctctgtcg ctcggaccgt ccggcgacag gggcacacgg
     1861 cagcctgtgg tgttcaggga ggtcaaggtg gagacgcggc acggcggccg aaggccgacg
     1921 tgcccggcgc gtagaagttg gcacaacttc gtggatcgcg ccgctcggcg ttctaccttg
     1981 acgcagcggc caccgcgcag cgggggcgaa cccgcgggct acagtgagtg ccccgtcgga
     2041 cggtcaaaac ccaaccacac cgcgacagct cgagcgccac gcgctcgacc cggctcgtca
     2101 gtcacaagca tcgtcgtgac tgttcaactg cgggtctcac caggtgcata tggtcaacgt
     2161 gtcgagggct cctgtgaaga acgatggacg gccgtccgcg tcaaggagcc acac
//
LOCUS       DASRKH010000370         2879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_6447, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000370 DASRKH010000000
VERSION     DASRKH010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6447"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="VFC06_05925"
     CDS             complement(<1..817)
                     /locus_tag="VFC06_05925"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZL81455.1"
                     /translation="MRVLALSVYLPTLLAEIGIGATLPLFALSALALGEPAAVASIAV
                     AVYSGGRMAGSALGGVLATRRGPIPATFAAYTVLASGAVLSATAVHLSMLALGVAIIG
                     IGHGGVHVARQAHIDAVAPLRIRARSLTTLAGIWRIGNFVGPFVGAAIIQASGLPAAY
                     VFAAVMVCLGILSLVFTAPRHLSIPRSQRAHVRVAEVLKPQLGVMSTLGVGVLLMGAL
                     RQARVVVIPLWAVHIGMSDSGASLVFGLATAVDMAMFIPAGYVMDRFGRVWTAV"
     gene            complement(843..1859)
                     /locus_tag="VFC06_05930"
     CDS             complement(843..1859)
                     /locus_tag="VFC06_05930"
                     /inference="COORDINATES: protein motif:HMM:NF022674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3027 domain-containing protein"
                     /protein_id="HZL81456.1"
                     /translation="MAASVEARVTDAARAAVVATAGDESSVGPMLGSEPGADGLISVR
                     FACTLRGYNGWEWSADLAVIDGTVTVCESALLPGGGALLAPTWVPWSERVQPGDLEAG
                     MILPYQAEDQRLIPGYTETGDEDRDAIANFEFGLGRERVLAPEARESAAERWFRGSHG
                     PTAASAVAAGAPCATCAFLVPVSGSMRTMFGVCANEWSPSDGRVVSMDHGCGAHSQTD
                     AERRASEWPAVDPVIDSAALDVLDLLTPEPEPEPEPEPEPEPEPEPEPEPEPEPEPEQ
                     DAVPSQDSEPKAEGEPETVTGPNPDGEPLAEPAVSADAEDSSAPEATPQDAEQDKAPE
                     TPTE"
     gene            complement(1859..2242)
                     /locus_tag="VFC06_05935"
     CDS             complement(1859..2242)
                     /locus_tag="VFC06_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HZL81457.1"
                     /translation="MPTGRVKWFDADKGFGFIASEEGDEVFLHASALPEGVSSPRQGT
                     KVEYSVVDGRRGPSAMSVIITEEAPSVAQAQRKPTDDMTMIVEDLIKMLDGVSNSLRK
                     GRYPDAVKGKQVASVLRAVAEQLDA"
     gene            2359..>2879
                     /locus_tag="VFC06_05940"
     CDS             2359..>2879
                     /locus_tag="VFC06_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZL81458.1"
                     /translation="MTQQPARILVVDDEPDLRDLLATSLKFAGYATESVASGRAALDH
                     LLHSAADLVVMDVMMPTMDGFTTVRKLRARGDNVPVLFLTARDAPEDALAGFAVGADD
                     YVTKPFSLAEVAARIEAILRRARPASPDATVLNVADLTLDDDAHEASRAGIPLDLTPT
                     EFALLRYLMANPGT"
BASE COUNT          491 a         1023 c          944 g          421 t
ORIGIN      
        1 ggacggccgt ccacacgcgg ccaaagcggt ccatgacata cccggcaggg atgaacatcg
       61 ccatgtcgac ggcggtcgcc agcccaaaga ccagcgaggc tccggagtcg ctcatcccga
      121 tgtgtacggc ccacaacgga atcacgacca cccgtgcctg ccgcaaggca cccatgagga
      181 gcaccccgac gcccagcgtg gacatcacgc ccagctgcgg cttcagcacc tcggccacgc
      241 gcacgtgagc gcgttgggag cgcgggatcg ataggtgacg cggcgccgtg aagaccaggg
      301 acaggatgcc gagacacacc atgacggccg cgaacacgta ggcggccggc aaccccgagg
      361 cttgaatgat ggccgcgccg acaaagggtc cgacaaagtt tccgatgcgc catatgccgg
      421 cgagcgtcgt caggctacgc gcacggattc tgagcggcgc cacggcgtcg atgtgcgcct
      481 ggcgggccac gtggacgccg ccgtgtccga tgccgatgat ggccacgccg agcgccaaca
      541 tagagaggtg aaccgcagtc gcagacaaca ctgctccgct ggctaggact gtgtacgcgg
      601 caaacgtggc ggggatgggg ccgcgacggg tggccaaaac cccgccgagc gcggaacccg
      661 ccatgcgacc acccgaatac acggcgacgg cgatcgacgc gacggcggcg ggttcgccca
      721 gcgccagggc cgacagggcg aacagcggca aggtcgcgcc gatgccaatc tcggcaagga
      781 gggtcggcag gtacaccgac agcgcgagaa cgcgcacaac gcccggatcg ggcgcttcct
      841 tcctactcag tgggcgtctc cggcgccttg tcctgctccg catcctgcgg ggtcgcttcg
      901 ggtgcggagg agtcctcggc gtcggcgctc accgcgggct ccgcgagggg ctcgccgtcc
      961 gggttcgggc ccgtcaccgt ctcgggctcg ccttcggcct tgggctcaga gtcctgcgaa
     1021 ggcaccgcgt cctgctctgg ctccggctct ggctccggct ccggctctgg ctccggctcg
     1081 ggctctggct ctggctcggg ctctggctct ggctctggct cgggagtgag caagtcgagc
     1141 acgtccaacg ctgcggagtc gatcacagga tccaccgccg gccactcgga agcacgcctc
     1201 tcggcgtcgg tctgcgaatg cgccccacag ccgtggtcca tgctcaccac gcgtccgtcg
     1261 gagggcgacc attcgttggc gcacacgccg aacatcgtgc gcatcgaacc cgatacgggc
     1321 accaagaacg cgcacgtcgc acacggtgca cccgccgcga ccgcggaggc cgccgtcggg
     1381 ccgtgggaac cgcggaacca gcgctcggcg gcagattcgc gcgcctcggg cgcgagaaca
     1441 cgctcgcggc ccagtccaaa ctcgaaattc gcgatcgcat cgcggtcttc atcgcccgtc
     1501 tcggtgtagc cggggatgag tctctggtct tccgcctgat agggcaggat catgccggct
     1561 tcaagatcgc ctggctgcac gcgctccgac cacggcaccc acgtgggggc gaggagggcg
     1621 ccgcctccgg gaagcaacgc cgattcgcac accgtcacgg tgccgtcgat gaccgcgagg
     1681 tcggcagacc attcccagcc gttgtagccc cgcaatgtgc aggcgaagcg cacggaaatg
     1741 agaccgtcgg cgcccggctc actaccaagc atcggcccca cggacgactc gtcgcccgct
     1801 gtagccacga cggctgcgcg ggccgcatcg gtcacgcggg cctcgactga ggcggccatc
     1861 aggcgtccaa ctgctctgcg acggcgcgca agacagatgc cacctgcttg cccttcaccg
     1921 cgtcgggata acgtcccttt cgcagtgagt tggagacgcc gtcaagcatc ttgatcaggt
     1981 cctcgacgat catcgtcatg tcatctgtgg gcttgcgctg cgcttgggca acggagggcg
     2041 cctcctcggt gatgatgacg ctcatcgccg aaggcccgcg gcgaccatcc accacggaat
     2101 actcgacctt ggtgccctgg cgaggggaag acacaccctc tggaagcgcg gaggcgtgca
     2161 aaaacacctc gtcgccctct tcgctggcga tgaagccgaa acccttgtcg gcgtcaaacc
     2221 acttcactcg tccggttggc acgtgcacct ctcccacgcg ccgcaggcgc agcgcgtagg
     2281 ccaagtctac aagggcaacg ggctatccac gggcgcgctt cccaggtatc tctcaggccg
     2341 gggcgccaca ctagacgcat gacgcagcag ccagcccgca tcctggtggt cgacgacgaa
     2401 ccggacctcc gcgacctcct cgcgacgtca ctcaaattcg cgggatacgc gacggagtcc
     2461 gtggcgtcgg gtcgggccgc ccttgatcac cttctgcact ctgcggcgga cctcgtggtg
     2521 atggacgtga tgatgccgac gatggacggc ttcacgaccg tgcgcaaact gcgggcccgc
     2581 ggcgacaacg tgcccgtgct gttcctgacc gcgcgcgacg caccggaaga cgccttggct
     2641 ggcttcgccg tgggcgcgga cgactacgtc accaagccgt tctctcttgc cgaggtggcg
     2701 gcgaggattg aggcgatcct gcgccgcgcg cggcccgcct cccccgatgc gaccgtgctg
     2761 aacgtcgccg acctcacgct cgacgacgac gcccacgagg cgagccgcgc gggcatcccc
     2821 ctcgacctca cccctaccga gttcgcgctg ctgcggtacc tcatggcgaa tcccgggac
//
LOCUS       DASRKH010000371         3426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_33396, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000371 DASRKH010000000
VERSION     DASRKH010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33396"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            241..1563
                     /locus_tag="VFC06_05945"
     CDS             241..1563
                     /locus_tag="VFC06_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HZL81459.1"
                     /translation="MSEPRRVVIIGAAGRDFHNFNVLHRNNPDARVVAFTAAQIPDIA
                     GRVYPASLAGSLYPDGIPIVDESELTDLIRRERVDEAVFSYSDVTHEHVMHLASACIA
                     AGADFVLPGGRPTMIRSTKPVIAVTAVRTGSGKSQTSRYLVHILKDMGKRVVSIRHPM
                     PYGDLAAQACQRFASYADLDTHDCTIEEREEYEPHIDLGFVIYSGVDYEQILRAAEQE
                     ADVILWDGGNNDLPFYASDLHIVVVDPLRPGDETRYHPGEANLRMADVVVVNKCDSAT
                     AENIAAVEASSRALNPDATIIRADSPVTVSDPTVVRGKRVIVIEDGPTLTHGSMTYGA
                     GVVGARQAGVAEIVDPSPFAVESIAATYRKYPNAVGILPAMGYGEAQIRDLEATIEAA
                     DCDAVVSGTPIDLTRVLSVSKPMVRATYVLREREPGALEREVRRVLEG"
     gene            complement(1588..3213)
                     /locus_tag="VFC06_05950"
     CDS             complement(1588..3213)
                     /locus_tag="VFC06_05950"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: protein motif:HMM:NF010052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HZL81460.1"
                     /translation="MAHEEVTRRGVQLLHDPLRNHGTAYTDDERGRLGLRGLLPVEVI
                     PQDVQVDRVLENFHRKPDDLERYILLTSLQDRNESLFYRVVTENMDLMMPIIYTPTVG
                     QACQEYCHIFRRPRGMYISAKDRGRVAELLANWPHDDVRAIVVTDGERILGLGDLGAN
                     GMGIPVGKLSLYTACAGIHPAWTLPLMLDVGTNNETLLQDRFYAGIRAPRLRGDAYDE
                     LVEELVQAVTERFPRALLQFEDFANANAFRLLRTYRDRICTFNDDIQGTAAMALAGVL
                     SALRLTGGRLADQRVLFFGAGEAGIGIADLLVSAIAADELTTAEARARCWFVDSQGLV
                     VSSRTELAAHKQPYAHDAAPVGDLLGAVAAVRPTILIGASGQKGAFTPDVVRLMAEYN
                     ERPVVFALSNPTSKAECTAVQAYESSEGRAIFASGSPFAPVEFQGRTLVPGQGNNAYI
                     FPGVGLGVIAVEASHVTDEMFMAAAHTLAGQVAEDDLAAGRVYPPLQEIRDISVDIAV
                     AVAEVAYAQGRAGLPRPEDLREHIRAQMWSPVY"
BASE COUNT          519 a         1182 c         1220 g          505 t
ORIGIN      
        1 cggcgggacg gggggcaccg ccgccgcgga ggactccacg gcggatgtcg ctgatgtgtg
       61 caggtcggtc acccgccaca gcctaccgac ctttccgttt cgcaaagaat cggtctgagt
      121 gagcgccccg gccgggcgcc gcagtggagg cggtcgccgg gcggcgcagt ggaggcggtc
      181 gccgggcggc ggcgatagcc ttggtccgcc gccgatgtca ccaagccatg aggagttccg
      241 atgagtgagc cacgcagggt agtcatcatc ggcgccgccg ggcgggactt ccacaacttc
      301 aacgtcctcc accgcaacaa ccccgacgcc cgcgtggtgg cattcaccgc cgcccagatc
      361 cccgacatcg cggggcgtgt gtacccggcg tcgcttgcgg gatccctgta cccggacggc
      421 atcccgatcg tggacgagtc cgagctcacc gacctgatcc ggcgcgagcg ggtggacgag
      481 gcggtcttct cctactccga cgtcacccac gagcacgtga tgcaccttgc ctcggcgtgc
      541 atagccgccg gcgccgactt cgtgctcccg ggcggccggc ccaccatgat caggtcgacc
      601 aaaccggtga tcgcggtgac cgcagtccgc accggctccg ggaagtccca gaccagccgg
      661 tacctcgtac acatcctcaa ggacatgggc aagagggtcg tctccatccg gcaccccatg
      721 ccctacggtg acctcgccgc gcaggcctgc cagcggttcg ccagctacgc cgacctggac
      781 acgcacgact gcacgatcga ggagcgcgag gagtacgagc cgcacatcga cctgggcttc
      841 gtgatctact cgggcgtcga ctacgagcag atcctgcgcg cggccgagca ggaggcggac
      901 gtgatcctgt gggacggcgg caacaacgac ctgccgttct acgccagcga cctgcacatc
      961 gtggtggtgg acccgctgcg cccgggtgac gagacccgct accacccggg tgaggcgaac
     1021 ctgcggatgg cggacgtcgt cgtcgtcaac aagtgcgact cggccaccgc ggagaacatc
     1081 gcagcggtcg aggccagcag ccgggcgctc aacccggacg cgacgatcat ccgcgccgac
     1141 agcccggtca ccgtctccga cccgaccgtg gtgcgcggca agcgggtgat cgtcatcgag
     1201 gacggcccca ccctgaccca cgggtcgatg acgtacgggg ccggcgtcgt cggcgcacgt
     1261 caggcgggtg tcgcggaaat cgtcgacccg agcccgttcg cggtcgagtc gatcgccgcc
     1321 acctaccgga agtacccgaa cgcggtggga atcctgcccg cgatgggcta cggcgaggcg
     1381 cagatccggg acctggaggc gaccatcgag gccgccgact gcgatgccgt cgtttcggga
     1441 acgccgatcg acctcacccg cgtgctgtcg gtgtccaagc cgatggtgcg cgccacctac
     1501 gtgttgcgcg aacgcgagcc cggggccctg gagcgggagg tccgcagggt ccttgaaggc
     1561 tgaccctcca gctcccgggc ctggttgtca gtagaccggg ctccacatct gggcgcggat
     1621 gtgctcgcgg aggtcctccg gccggggcag gccggcgcgg ccctgcgcgt aggccacctc
     1681 ggcgacggcg acggcgatgt ccaccgagat gtcgcggatc tcctgcaggg gtgggtagac
     1741 gcgcccggcg gccaggtcgt cctccgccac ctggcccgcc agggtgtgcg ccgccgccat
     1801 gaacatctcg tcggtcacgt gggacgcctc gacggcgatc acgccgagcc ccacgcccgg
     1861 gaagatgtag gcgttgttgc cctgtcctgg cacgagggtg cggccctgga actccaccgg
     1921 ggcgaacggg ctcccggagg cgaagatggc gcgcccctcg ctcgactcgt aggcctgcac
     1981 ggcggtgcac tccgccttcg aggttgggtt ggagagcgcg aagaccaccg gccgctcgtt
     2041 gtactccgcc atcagccgca ccacgtccgg ggtgaaggcg cctttctggc cggaagcccc
     2101 gatgaggatg gtcggccgga ccgccgcgac ggcgcccagc aggtccccca ccggtgcggc
     2161 gtcgtgggcg tagggctgct tgtgggccgc cagttcggtg cggctggaca ccacgaggcc
     2221 ctgggagtcg acgaaccagc agcgggcgcg ggcctccgcc gtcgtgagct cgtccgccgc
     2281 gatggcggag acgaggaggt cggcgatccc gatgccggcc tcgccggccc cgaagaacag
     2341 cacgcgctgg tccgccaagc gccccccggt gaggcggagc gcggagagaa cgcccgcgag
     2401 cgccatggcg gcggtgccct ggatgtcgtc gttgaaggtg cagatccggt cgcggtacgt
     2461 gcgcagcagc cggaacgcgt tggcgttcgc gaagtcctcg aactggagca gcgcgcgcgg
     2521 gaagcgctcc gtcacggcct ggaccaactc ctccacgagc tcgtcgtagg cgtcgccgcg
     2581 gaggcgcggc gcgcggatgc ctgcgtagaa ccggtcctgg agcagggtct cgttgttcgt
     2641 gccaacgtcc agcatcaggg gtagggtcca cgcggggtgg atgcccgcgc acgcggtgta
     2701 cagcgagagc ttgccaaccg ggatgcccat cccgttcgcg ccgaggtcac cgaggccgag
     2761 gatgcgctcg ccgtcggtga ccacgatcgc gcggacgtcg tcgtgcggcc agttggccag
     2821 gagctcggcg accctgccgc ggtccttcgc gctgatgtac atcccgcggg gacggcggaa
     2881 gatgtggcag tactcctggc aggcctggcc cacggtgggg gtgtagatga tgggcatcat
     2941 caggtccatg ttctccgtga ccacccggta gaacagggac tcgttgcggt cctgcaggct
     3001 ggtgagcagg atgtaccgct ccaggtcatc gggtttgcgg tggaagttct ccagcacgcg
     3061 gtccacctgg acgtcctggg gaatcacctc gacgggcagg agccctctca ggcccagccg
     3121 cccacgctca tcgtcggtgt aggcggtccc gtggttgcgc agcgggtcgt gcagcagttg
     3181 gacgccccgg cgcgttactt cttcgtgtgc cacccctcca cggtattcct ccgtccggat
     3241 gcacgaacgg tgttcgcagc ccttcgatca gtaggccggg ctccacacgt acgcgcggat
     3301 gtgggcttcg aggtcctcgg ggcgcgggac gcgggcgagc ccctgagcgt acgccacctc
     3361 ggcgacggcg acggcgatcg ccacggagac ctcgtggatc tcccggagcg gcgggtacac
     3421 gctgcc
//
LOCUS       DASRKH010000372         3040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_47037, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000372 DASRKH010000000
VERSION     DASRKH010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47037"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..613
                     /locus_tag="VFC06_05955"
     CDS             113..613
                     /locus_tag="VFC06_05955"
                     /inference="COORDINATES: protein motif:HMM:NF013430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZL81461.1"
                     /translation="MFDTMAFTDGLLGSGGAQGGLSEYVVVYEAQQGRQLRTIPSDIP
                     WEVAALNEPMAVARHAVNRLGLQPGATVAVFGAGPIGLGAVLSAKALGASHVVAVDVQ
                     PNRLETELKVGADAVINSAEEDVKARLIELHGTAPRSFVRGARPPTATSTPPACPRSS
                     RRSSPP"
     gene            577..879
                     /locus_tag="VFC06_05960"
     CDS             577..879
                     /locus_tag="VFC06_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81462.1"
                     /translation="MPAVIETILSSVKHGAVVSIPAVHKAPVPVNFGDLLTTEVDIRL
                     AMGYPTEIFEVTDDIIANADKYRHIISHVIPFDRALEAIELARTPGAADKVVVVLE"
     gene            884..1039
                     /locus_tag="VFC06_05965"
     CDS             884..1039
                     /locus_tag="VFC06_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81463.1"
                     /translation="MKNPVHPGEILREDVLADLGLTVGEALQSAHDLAAERAAGLPTV
                     RRLDVVA"
     gene            complement(1102..1314)
                     /locus_tag="VFC06_05970"
     CDS             complement(1102..1314)
                     /locus_tag="VFC06_05970"
                     /inference="COORDINATES: protein motif:HMM:NF022596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HZL81464.1"
                     /translation="MGRHLGPVLVVLGIVVVVVGLLAWTGALSWFGRLPGDIRVEREN
                     VRIYVPWVSMLLISAVASLILWVLRR"
     gene            1493..2020
                     /locus_tag="VFC06_05975"
     CDS             1493..2020
                     /locus_tag="VFC06_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81465.1"
                     /translation="MALSGRTRRRFSDASHLAAENLLEKAYRAWREHPPRAAAYVDKA
                     ARLPYDDHERVFPVAAAAIMRLFDLLVDELEDADEDEDLWLDVALTVLRGTTSSARFD
                     MRDMLLALDQDWNDVERRHRKIRAASELIPPQTALRELQDLTPDHLRDRVMAILELCV
                     TYEEELEEAWLAVEG"
     gene            complement(1934..2617)
                     /locus_tag="VFC06_05980"
     CDS             complement(1934..2617)
                     /locus_tag="VFC06_05980"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HZL81466.1"
                     /translation="MRQRLRDREVGVLFARPGQAEGQQVPDPFFGGRGPARTTCNGCG
                     ACMTGCRVGAKNTLVKNYLWLAETHGCEIIPLTTVRRVRPLPDDGGYRVDIESTSRPG
                     RGRRTIRADQVVFAASALGTQGLLHTMKEEGFLPHLSDRLGALSRTNSEAILGVTAPD
                     RSVDYSRGPAITSSFHPDEHTHVEPVRYGKGAGAALIGASAFDREPSLLEFLLIRDAQ
                     LEDRHDAVA"
BASE COUNT          493 a         1004 c         1082 g          461 t
ORIGIN      
        1 cactacggcg ggatcccgcc gcacgtcgga gccacgcggc ttggccacga acccgtgggc
       61 gaggtcgtgg aggtcggcag cgaggtgagc ggattcgccg tcggaccacg tcgtgttcga
      121 caccatggcc ttcacggacg gcctgctggg gagtggcgga gcccagggcg gactgtccga
      181 gtacgtcgtc gtgtacgagg cgcagcaggg caggcagttg cgcaccattc cgtcggacat
      241 cccgtgggag gtggccgcgc tcaacgagcc catggccgtg gcccgccacg cggtgaaccg
      301 cttgggcctg cagcctggtg cgacggttgc ggtcttcggc gcaggaccca tcggcctcgg
      361 tgcggtgctc agcgcgaagg cgctgggtgc cagccacgtg gtcgccgtgg acgtgcagcc
      421 gaaccgcctg gagacagagc tcaaggtggg tgccgacgcc gtcatcaact ccgcggagga
      481 agacgtcaag gcccgcctga tcgaactcca cggcacagcc ccgcggagct tcgtccgggg
      541 cgcgcggccg ccgacggcta cctcgacgcc gccggcgtgc ccgcggtcat cgagacgatc
      601 ctctcctccg tgaagcacgg tgcggtggtc tcgatcccgg cggtccacaa ggcgccggtg
      661 ccggtgaact tcggggacct cctcaccacc gaggtggaca tccgcctcgc gatgggctac
      721 cccacggaga tcttcgaggt caccgacgac atcatcgcca acgccgacaa gtaccggcac
      781 atcatcagcc acgtgatccc cttcgaccgt gcgctggagg ccatcgagct ggcgcggacg
      841 ccgggcgctg ccgacaaggt ggtcgtggtc ctggagtgag gagatgaaga atccggtaca
      901 ccccggtgag atcctgcgcg aggacgtgct ggcggacctc ggcctgactg tcggcgaggc
      961 cctgcagtcc gcccacgacc tcgctgcgga gcgggccgcc ggcctgccca cggtccgtag
     1021 gctcgacgtc gtggcctgaa cgaacacacg ccccggccgg ccgagtcgct gacgagtgag
     1081 tccgtccccg cacggagcga atcagcgccg gagcacccag aggatcaggc ttgcgacggc
     1141 cgagatgagc agcatcgaga cccagggcac gtagatgcgg acgttctcgc gttccacccg
     1201 gatgtcgccg gggagccgtc cgaaccagga cagtgcgccg gtccatgcca gcagtccgac
     1261 gaccacgaca acgattccga gcaccaccag caccggaccc aggtgtcgcc ccatgtgtcc
     1321 atcctgacgg atgggtagtc cgtgcgctgt cccccgcagt cgtgacagcg ctctttgtgg
     1381 acaccggcgc gcaggacctt gtcgtcggac taccctctcc cgtgtggacg aggatgaggt
     1441 tttcgagcgt tatcgggctc tattgaggtc gacgtcggca ttcggcgatg cattggcact
     1501 gtcggggcgg acgcggcggc ggttcagcga cgcatcccac ctcgccgccg aaaacctgct
     1561 ggagaaggct taccgggcgt ggcgggagca cccgccgcga gccgctgcat acgtcgacaa
     1621 ggcggccagg ctgccgtacg acgaccacga gcgggtgttc ccggtggctg ccgccgcgat
     1681 catgcgcttg ttcgacctgc tcgtggatga gctggaggac gccgatgagg acgaggacct
     1741 gtggctcgac gtcgccctga cggtgctgcg ggggaccacg agcagcgcgc gtttcgacat
     1801 gcgggacatg ctgctcgcgc tcgatcagga ctggaacgac gtggaacgcc ggcaccggaa
     1861 gattcgggca gcaagcgaac tgattccccc gcagaccgca ctgagggagc tgcaggatct
     1921 caccccagac catctacgcg accgcgtcat ggcgatcctc gagctgtgcg tcacgtatga
     1981 ggaggaactc gaggaggctt ggctcgcggt cgaaggctga agcaccgatc agggcagcac
     2041 cggcgccctt gccgtagcgg acgggctcca cgtgggtgtg ctcgtccgga tggaaggagg
     2101 aggtgatggc cggcccgcga ctgtaatcca cggaccgatc gggcgcggtg acgcccagga
     2161 tcgcctcgga gttggttcgg gagagggcgc cgagccggtc ggagaggtgg gggaggaagc
     2221 cctcctcctt cattgtgtgc agcaggccct gggtgccgag cgccgaggcg gcgaacacca
     2281 cctggtcggc gcggatcgtc cggcgacccc gccccggacg ggaggtggac tcgatgtcca
     2341 cccggtaacc gccgtcgtcg ggaaggggac gcacccgccg caccgtggtg agcgggatga
     2401 tctcgcagcc gtgggtctcg gccagccaga ggtagttctt gacgagggtg ttcttggcgc
     2461 cgacgcggca ccctgtcatg caggcgccgc acccgttgca ggtggtgcgc gcgggacccc
     2521 ggccgccgaa gaacggatcg gggacctgtt ggccctcggc ctggcccggc cgggcgaaca
     2581 ggacgccgac ctcccgatcc cgaaggcgct gccgcaacct ccgtccgaat ggaaggtccc
     2641 gtacgggcga ttggcgacat cactcggact ggagcgaggt ctgaccagtg ggtacgagat
     2701 cgctgccgct ctggctcatt cagtcgccgg ccggaacgcg accgcaggga agcgggtggc
     2761 ggccgcgcgc gccgtcgtcg tcgacgtctc gcggtccagg cggagccgtg gggcgagcgt
     2821 ccgccacgcg cgggtaccgg cgcccgtagc cctcgatgcg ccgagaggct accgctgatt
     2881 ggttgtgcac aaccgataca gtcgcgccgt tgtgtatcgg acccttgcct tcgcgttacc
     2941 gcgaaaccgc caaattgctc gcaatgggtc agccggttcc tgtatccagc gatactgtcg
     3001 cgcagccgtg ggtgctggat gtatcgcatg cccgttggac
//
LOCUS       DASRKH010000373         4953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_51582, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000373 DASRKH010000000
VERSION     DASRKH010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4953
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51582"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="VFC06_05985"
     CDS             complement(<1..550)
                     /locus_tag="VFC06_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HZL81467.1"
                     /translation="MAGLGLERELATYLAHVTVERGLSRNTIAAYRRDLGRYVAYLDA
                     QDLASVADASPHHVSDFAQAVSSGDDGRTALAPASAARTVVAVRGWHRFAVAEGWTID
                     DASIDVKPRTAPARLPKALSTDAVESLLEAAGVGEGLGPLRDKALLEVLYASGARVSE
                     AIGLDVDDISLEAGAESVLLRGK"
     gene            complement(538..1500)
                     /locus_tag="VFC06_05990"
     CDS             complement(538..1500)
                     /locus_tag="VFC06_05990"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011774786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HZL81468.1"
                     /translation="MPSTTTTPPDVPAPSVGRILAATRVLKAYVSLTKPRIIELLLIT
                     TLPTMVLAADGWPGAWLMIATLIGGTLSAGSANAFNSYLDRDIDAVMKRTQGRPLVTG
                     AVSPRAGLTFAWILAAVSTAWFWWLVGSPLATLLSVIAIVLYAVGYTMLLKRRTSQNI
                     VWGGAAGCMPVLIGWASVRESLSWTPVVLFLIIFLWTPPHYWPLALKFRKDYEHAEVP
                     MLPVVATPVKVAKEMIAYAAAMVVVSLALVPLASMTWIYAVLALGFGVWFLGGCVALY
                     RRSVDGAKLGSMRLFRDSIMYLSVVFGAILADPFWPDSLAVWQG"
     gene            1609..3669
                     /gene="tkt"
                     /locus_tag="VFC06_05995"
     CDS             1609..3669
                     /gene="tkt"
                     /locus_tag="VFC06_05995"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HZL81469.1"
                     /translation="MATGWTDLDNRAIDTLRVLAADAVEKVGNGHPGTAISLAPAAYL
                     LFQNVMKHDPSDPNWLGRDRFVLSCGHSSLTLYLQLFASGYPLSLDDLKKLRTEGSQT
                     PGHPEYGHTPGVEITTGPLGSGLASAVGMAMASRRERGLLDPDAAPGDSVFDHSFYVI
                     ASDGDLQEGVSSEASALAGVQELGNLIVIWDDNKISIEHETDIAFDEDVLKRYEAYGW
                     HTQRVDWVTPDGYKEDGDALLDALESAKAETSKPSIIALSTIIGWPSPSKQNTGGVHG
                     SALGAEEVAGLKKVLGFDPEQNFAVEAKVLSHMQEVRGRGQAAHSEWNGRFSRWQAEQ
                     PERAALLDRLVAKELPANWTDALPTYEVGTKIATRAASGEVLTALAPVLPELWGGSAD
                     LAGSNNTTMKGELSFLPPHRSVKSWEGTWYGRTLHFGIREFGMGTILNGITLHGLTRV
                     YGGTFLVFSDYMRGAVRLAALMKIPTTFVWTHDSVGLGEDGPTHQPIEHVATLRAIPG
                     IDIVRPADANETAFAWKHALETKDRPCGLILTRQGVPTLEGTSAEGFAKGAYVLAGAD
                     EDADVLLIATGSEVQLAVAAREALAKDGIKARVVSMPCRELFERQDAAYRDSVLPSSV
                     KARVSVEAGSPLGWREIVGDAGRVIGIDHFGESADGGLLLKKYGFTAENVVSKAKESV
                     EASA"
     gene            3666..>4953
                     /locus_tag="VFC06_06000"
     CDS             3666..>4953
                     /locus_tag="VFC06_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HZL81470.1"
                     /translation="MSTFLSTWVGLDNEAHLAVTVAGDAAEAASGLTDSIVADKVASR
                     IANGDPTLWGPHAEAEASDRLGWVTLPLSSRELIEDLENLYVDLRADGINRIVLAGMG
                     GSSLGPEVICATYDVPLVTLDSTDPDQVREALTGDLESTLLVVSSKSGTTVETDSLRR
                     VFESAFEESGLDPRKHIVAVTDPGSPLHKTAELAGYRAVILADPHVGGRYSVLGAFGL
                     VPSALAGVPVAEILDEADAVSDSLAEDHAANPAIVLGAAMAGSPRRTLVLSDHDSGLV
                     GFADWAEQLIAESTAKGGVGVLPVVAEVDAPDALADGALDTRFVALDFDGEPEGHSIW
                     ISGTLGGQLLLWEYATAIAGRLLGINPFDQPNVESAKTATRSLLDSLPEPEPPAFVDG
                     GVEVRGSAELLEGLTTLEAAIAAIKAAVPADGYLAVM"
BASE COUNT          873 a         1699 c         1603 g          778 t
ORIGIN      
        1 ccttgcccct cagcagcacg gactccgcac ccgcttccag cgagatgtcg tccacatcaa
       61 gaccgatcgc ctccgaaacc ctcgcgcccg acgcgtagag gacctccagc aacgccttgt
      121 cgcgtagcgg ccccaggccc tcccccactc ccgccgcttc gagtaggctt tccaccgcgt
      181 cggtgctgag ggccttgggg agccgggctg gagcagtgcg cggcttcacg tcgatcgacg
      241 catcgtcgat cgtccagcct tcggcgacgg caaagcggtg ccagccgcgc acggccacca
      301 cggtacgggc ggccgatgcg ggggcaaggg ccgtgcgccc gtcgtcgccc gacgacacgg
      361 cctgcgcgaa atccgagacg tggtgggggc tcgcgtcggc gacggaggcc agatcctggg
      421 cgtccagata tgcgacatag cgccccagat cgcggcgata cgcggcgatc gtgttgcgcg
      481 atagccctcg ctcgacggtc acatgagcga gatacgtggc gagctctcgc tcgagtccta
      541 gccctgccac accgcgagcg agtctggcca gaacgggtcg gccaagatgg cgccaaagac
      601 gacggagaga tacatgatcg agtcgcggaa caggcgcatc gagcccagct ttgcgccgtc
      661 gacggagcgc cgatatagcg cgacacaacc gcccaggaac cacacgccaa atccgagcgc
      721 caggaccgcg tagatccacg tcatcgaggc gagcgggacg agcgccaggg acaccaccac
      781 catggcggcg gcgtacgcga tcatctcctt ggcaaccttc acgggcgtgg cgaccaccgg
      841 cagcatcggc acctcggcgt gctcataatc cttgcggaac ttgagcgcga gcggccaata
      901 gtgcggcggc gtccacagga agatgatgag gaacagaaca acgggcgtcc aggacagcga
      961 ctcgcgcacg gaagcccagc caatcaagac gggcatgcaa cccgccgcac caccccacac
     1021 gatgttctgg gaggtacgac gcttcagcag catcgtgtag cccacggcgt agagcacaat
     1081 cgcgatcacc gacagcagcg tggcgagggg cgaacccacc agccaccaga accacgcggt
     1141 cgagacggcg gcgagaatcc acgcgaacgt cagccccgct cgcggagaga cggcccccgt
     1201 cacaagcggc cttccttgcg tgcgcttcat caccgcgtcg atgtcgcggt caagatagct
     1261 attgaacgca ttcgccgagc ctgcggacag ggtcccaccg atcagagtgg cgatcatcag
     1321 ccacgcaccg ggccagccat cggccgcgag caccatggtg ggcaacgtcg tgatgagcag
     1381 tagctcgatg atgcggggct tggtgagcga cacatacgcc ttgagcacgc gggtcgcggc
     1441 gagaattctg ccaacggagg gtgcagggac gtcggggggc gtcgtcgtag tggacggcat
     1501 tgctcgattg tacgtgggct gtgcacaaac aagcgcccga gcgcgcccac ctggcccgct
     1561 ccgccggtag gctaacaaag ccgcccaacg aagccttaag ggagaaccgt ggccacagga
     1621 tggactgacc tcgacaaccg cgcgattgac accctgcgtg tcctcgccgc cgacgcggtg
     1681 gagaaggtgg gaaacggcca ccccggaaca gcgatctcgc tcgctcccgc cgcctacttg
     1741 ctctttcaga acgtgatgaa gcacgacccc tctgacccca actggttggg tcgcgaccgt
     1801 ttcgtgctgt cgtgcggtca ctcgtcgttg acgctctacc tccagctctt cgcctcgggg
     1861 tacccgttga gccttgacga cctcaagaag ttgcgtaccg aaggctcgca gacccccgga
     1921 caccccgagt acgggcacac gcccggcgta gagatcacga cggggccgct cggctccggc
     1981 ctcgcgtccg cagtgggcat ggccatggcg tcacgccgtg agcgcggtct gctcgatccc
     2041 gacgccgcgc ctggcgattc ggtattcgac cacagcttct atgtgattgc ctctgacggc
     2101 gacctccaag agggcgtgtc ttccgaggcc tcggccctcg ccggagtcca ggagctgggc
     2161 aacctcatcg tcatctggga cgacaacaag atttccatcg agcacgagac cgacatcgcg
     2221 ttcgacgagg acgtcctgaa gcgctacgag gcctacggtt ggcacacgca gcgcgtcgac
     2281 tgggtcacgc ccgacggtta caaggaggac ggcgacgccc tccttgacgc cctcgagagc
     2341 gccaaggcgg agaccagcaa accctccatc atcgcgctca gtacaatcat cggctggccg
     2401 tcgccctcca agcagaacac tggaggcgtg cacggatccg cgctcggcgc agaagaggtt
     2461 gccggcctca agaaggtgct gggctttgac cccgagcaga acttcgcggt cgaggccaag
     2521 gtgctgtcgc acatgcagga agtgcgcggc cgcggacagg ccgctcattc ggagtggaac
     2581 ggccgcttct ccaggtggca ggccgagcag cccgagcgcg cagcgcttct tgaccgcctg
     2641 gtcgccaagg agcttccggc gaactggacc gacgcgctgc cgacgtatga ggtcggcacc
     2701 aagattgcga cgcgcgccgc ttctggcgaa gtgctgaccg ccctagcgcc cgtcctgccc
     2761 gaactgtggg gcggttccgc ggacctcgcc gggtcgaaca acaccaccat gaagggcgaa
     2821 ctgtcgttcc tgcccccgca ccgctcggtg aagtcgtggg aaggcacctg gtatggacgc
     2881 acgctgcact ttggcatccg cgagttcggc atgggcacca tcctcaacgg catcacgttg
     2941 cacgggctga cgcgcgtcta cggcggcacg ttcctcgtgt tctccgacta catgcgaggc
     3001 gcggtacgcc tcgcggcgct gatgaagatc cccaccacct tcgtgtggac ccacgactcc
     3061 gtcggcctgg gcgaggacgg ccccacccac cagccgatcg aacacgtcgc cacgctgcgc
     3121 gcgattcccg gcatcgacat cgttcgcccc gccgacgcca acgagactgc gtttgcgtgg
     3181 aagcacgctc tcgagaccaa ggaccgtccg tgcggcctca tcctcacgcg tcagggtgtt
     3241 cccacgctcg aaggaacgtc agccgaaggc ttcgccaagg gcgcctatgt gctcgcgggt
     3301 gccgacgagg acgccgacgt gctgctcatc gctacgggct ccgaggtgca gttggccgtc
     3361 gcggcgcgag aggccctcgc gaaggacggg atcaaagcgc gcgtcgtctc gatgccgtgc
     3421 cgcgaactgt tcgagcgcca ggatgccgcc tatcgcgact ccgtcctgcc ctcctctgtg
     3481 aaggcgcggg tgtccgtcga ggccggttcg ccgctcggct ggcgcgagat cgtcggtgac
     3541 gccggacgcg tcatcggcat tgaccacttc ggcgagtccg ccgacggcgg tctcctgctc
     3601 aagaagtacg gcttcaccgc cgagaacgtc gtctccaagg ccaaggagtc ggtggaggcc
     3661 tctgcgtgag cacgttcctg tccacgtggg tgggcctcga caatgaggcc cacctcgcgg
     3721 tcacggtagc tggagacgcc gctgaggcgg cttcggggct cacggacagc atcgtggccg
     3781 acaaggttgc ctcgcgcatc gcgaacggtg atcccacgct gtggggccct cacgccgaag
     3841 cagaggcaag cgatcgcctg ggctgggtga cgctacccct gtcctcacgc gaactcatcg
     3901 aggatctcga aaatctctat gtggacctcc gcgccgacgg catcaaccgg attgtcctcg
     3961 cgggcatggg tggctcgtcg cttggcccgg aggtcatctg cgccacgtat gacgtgcccc
     4021 tcgtgaccct ggactcgacc gatccggatc aggtgcgcga ggcgctcacc ggcgacctcg
     4081 aaagcacgtt gctcgtggtg tcctcgaagt cgggcaccac cgtggagacg gactctctgc
     4141 ggcgcgtctt cgaatcggcc ttcgaggagt ccgggctcga cccgcgcaag cacatcgtgg
     4201 cggtcacaga cccaggctcc ccgctccaca agacggcgga actggccggc taccgcgccg
     4261 tcatcctcgc ggaccctcat gtcggcggcc ggtactcggt tctcggcgcg ttcggacttg
     4321 tgccgtcggc cctggccggc gttccggtag cggagatcct tgacgaggcc gacgcggtgt
     4381 ccgactcgct cgccgaggat catgcggcaa accccgccat cgtgttgggt gccgccatgg
     4441 cgggctcacc gcgtcgcacc ctggtcctct ccgaccatga ttcggggctc gtgggattcg
     4501 ccgactgggc ggagcagttg atcgccgaat cgacggccaa gggcggcgtc ggagtccttc
     4561 ccgtcgtggc ggaggttgac gccccggacg ccctggcgga cggcgctctc gacactcgtt
     4621 tcgtcgccct cgactttgac ggagagccgg agggacactc gatctggatc tcgggcaccc
     4681 taggcggaca attacttctg tgggagtacg ccaccgccat cgccgggcgt ctgctcggca
     4741 tcaatccctt tgaccagccg aacgtggagt ccgccaagac cgccacacgt tcattgctcg
     4801 actcgcttcc cgagccggaa ccgccggcct tcgtggacgg cggagtagag gtacgcggtt
     4861 ctgcggagct cctggagggc ctcacgaccc tcgaggcggc gattgccgcc atcaaggccg
     4921 ccgtaccggc agacggctac ctcgcggtca tgg
//
LOCUS       DASRKH010000374         2586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_24684, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000374 DASRKH010000000
VERSION     DASRKH010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24684"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1584)
                     /locus_tag="VFC06_06005"
     CDS             complement(<1..1584)
                     /locus_tag="VFC06_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81471.1"
                     /translation="MVDYISRALVADALSRWRAHVSSRSTVSPLRNLTATQSAIIELE
                     HAHPSGLAQLFAGRATLLSTLVQDREEYAAASLRGRLILEESVAVAATTGMWTAALVV
                     GTVSWEGCEMPLLLRPISLDKARQDDLVVTLRHEAFLNPVFAAILRDRGGPTDLASRA
                     STTLEGREFDPRPLWAEVRDQAHLFDNLEVGERLLIGAFDDPEQRLLDDLDDLDPVIG
                     ISDVVAAIAGDEDARAILAETLPRWAAVERDPFAERGMGDLDDSSFFVLDMVALGRSI
                     AVDAPPGSDATAVVAAIAADAAASGRTALVVGGSDPALASVDAALASSGAGDIALTGV
                     VSAWNAESRARLLASLTLDTPEIDESHLRGLGERLLHARSDAAARYDALHRPHRPWGV
                     SAFETVQAIVRLTGQEPQPSTTVRLGTDAAAAVAEHGLGHVAAAIMERMHGEAQWPDE
                     PETDEGDEVAPWWHRVATSNEHGGELDEALAVLLRMVPRLRSDAQAAARDAGVDEAQT
                     LSVWREQVRLFNDVQVTLDT"
     gene            complement(1724..>2586)
                     /locus_tag="VFC06_06010"
     CDS             complement(1724..>2586)
                     /locus_tag="VFC06_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZL81472.1"
                     /translation="ATVATFPQFSPDEFLKTQKRRPGTFLPAVPPMLDRIAEAAEKSG
                     ADLSSFAVSLSGSMALPTATAERWERLTGGLVIEGYGLTETSPVAVGNPLSAARRPGT
                     LGIPYPSTEVRVVDPEDPTVEVPFGASGELLIRGPQVFSGYWNLPEETASQLLPGGWL
                     RTGDVVQFSDDGFIVLVDRIKELIVTGGYKVYPSQVEEHLRRMPGVKDVAVVGLPGGD
                     LGEKVVAAIVMEADHYAVGLDAVRDWCGEKFARYALPRGVVILPDLPRSQIGKVLRRV
                     VKLELMAPTP"
BASE COUNT          448 a          905 c          856 g          377 t
ORIGIN      
        1 ggtatcgagc gtgacctgca cgtcgttgaa gagcctcact tgctcgcgcc acacggagag
       61 ggtttgcgcc tcgtccacgc cggcgtcgcg cgcggcagcc tgagcatcgg accgtagccg
      121 cggcaccatg cgcagcaaga ccgcgagcgc ttcgtccagt tcgccgccgt gctcgttcga
      181 ggtggcgacg cggtgccacc acggggcgac ctcgtcgcct tcatccgtct cgggttcatc
      241 cggccactgc gcctcgccgt gcatccgctc catgatcgct gcggccacgt ggcctagtcc
      301 gtgttcggcg acggccgctg cggcatcggt cccgaggcgg acggtcgtgc tgggctgggg
      361 ttcctgaccc gtgagcctca cgatcgcctg caccgtctcg aacgcgctca cgccccacgg
      421 gcggtgcggc cgatgcaatg cgtcgtatcg ggccgccgcg tcggaccgcg catgcagcag
      481 tcgctcgccg aggccgcgca aatgagactc gtcgatctcc ggggtgtcga gtgtcaggga
      541 cgcgagcaac cgcgctcgcg actctgcgtt ccaggcggaa accactcccg tgagggcgat
      601 gtctccggcg ccggacgacg cgagggcggc gtcgacggac gcgagcgcgg gatcggaccc
      661 ccccaccacg agcgcggtgc gccccgatgc tgcggcgtcg gccgcgatgg cggcgaccac
      721 tgcggtcgcg tccgatccgg gaggggcgtc gaccgcaatc gagcggccca gcgccaccat
      781 gtcgagcaca aagaacgagg agtcatcaag atcgcccatg ccgcgctcgg cgaagggatc
      841 tcgctcgacg gccgcccacc ggggcagggt ctccgccaag atggcccgcg cgtcttcatc
      901 gcccgcgatc gcggccacca catcggagat gccgatgacg gggtccaggt cgtccaggtc
      961 atccaggagc cgctgttcag gatcgtcgaa ggcgccaatc aggaggcgct cgcccacctc
     1021 caagttgtca aacaggtgtg cctggtcgcg gacctcggcc cacagtggcc gggggtcaaa
     1081 ctccctaccc tcaagcgtgg tggaagcacg ggacgcgaga tcggtgggcc cgccgcggtc
     1141 gcgaaggatc gcggcgaaca ccgggttgag gaaagcctcg tgcctgaggg tgacgacgag
     1201 gtcgtcttgc cgcgccttgt cgagcgaaat gggccgcagc aggagcggca tctcgcagcc
     1261 ctcccacgag acggtgccca cgacgagcgc ggctgtccac atgccggtgg tcgcggccac
     1321 cgcgacagac tcttcgagaa tcaaccgccc tcgcagagac gccgccgcgt attcctcgcg
     1381 gtcctggaca agcgtggaca gcagcgtcgc gcgacctgcg aagagctgcg caagacccga
     1441 cgggtgtgca tgctcgagtt cgatgatggc cgactgggtc gcggtgagat tgcgcagtgg
     1501 cgagacggtc gagcgcgaac tcacatgagc ccgccaccgg ctcagcgcgt cggcgacaag
     1561 cgcccgcgag atgtagtcca ccacaaacct acggtaacga tgcggcaacc gacccaccgg
     1621 ctgccacgcc gcacccgccg atcacgcgac aatgcgcgcc gactcatgca gccgacgcgc
     1681 accgttggag gaattgtggc gccgccatgc cgctggcgtc aaactaaggg gtcggagcca
     1741 tcaattcgag cttgacgacc cgcctcaaaa ccttgccgat ctgcgaacgg ggaaggtcag
     1801 gaagaatcac cacaccacgg ggcagcgcgt acctcgcgaa cttctcgccg caccagtccc
     1861 gcaccgcgtc caagccaacg gcgtagtgat ctgcctccat cacgatggcc gcgacaacct
     1921 tctcgcctag gtcgccgcca gggaggccga ccacggcgac atccttcacg ccgggcatcc
     1981 gacgcaagtg ctcctccacc tgagacgggt acaccttgta tccgccggtg acgatcagtt
     2041 ccttgatgcg gtcgacgagc acgatgaagc cgtcgtcgct gaactgcacc acgtctcccg
     2101 tgcgcagcca cccgccaggc agcaactggg aggcggtctc ttctggcagg ttccagtacc
     2161 cggagaacac ttgtggcccg cgaatgagca actcgccgga ggccccaaag ggcacctcca
     2221 ctgtcgggtc ctccggatcc accaccctca cctcggtgct ggggtacggg atcccgagcg
     2281 tgccggggcg ccgcgcggcc gagagcgggt tgccgacggc cacgggggat gtctccgtca
     2341 ggccgtagcc ctcgatgacg aggccgcccg tcagcctctc ccaacgctct gcggtggccg
     2401 tgggaagagc catcgaccct gacagggaga ccgcaaagga gctcaggtcc gccccggact
     2461 tctcggctgc ctcagcaatc cgatcaagca tcggcggcac agcgggcagg aacgtgccgg
     2521 ggcgtcgttt ctgagtcttc aagaattcat cggggctgaa ctgcggaaac gtggccaccg
     2581 tggcgc
//
LOCUS       DASRKH010000375         2638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_62864, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000375 DASRKH010000000
VERSION     DASRKH010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62864"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..1692)
                     /locus_tag="VFC06_06015"
     CDS             complement(13..1692)
                     /locus_tag="VFC06_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013115615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZL81473.1"
                     /translation="MRIVIVGGVAGGMSAAARARRLDETAEIIVFEKGPYVSFANCGL
                     PYHVGGEITSQEALLLHTPETLRSSLGLDVRPNTEVTTIDRAAQTVTVVGPQGEQKVA
                     YDALVLSPGANAFMPPIPGVDLPQVTHLRTVDDAIALAERVTDARSAVVLGAGFIGLE
                     TAEALRERGLDVALIELAPQVLPPLSPELARLVELELTANGVDVITGSGAAAIEPAER
                     GVTVTLTNGAAATVDLVVVSVGVRPNSKLASDAGLEVDDRGAIIVDSQQRTVDPHIWA
                     VGDAIAVRHGVTGVVAPVPLAGPANRQGRRAAESIYGVAKDAKPVLGTAVVRVFGLTA
                     AVTGASPRMLDQAGMDYVVHHTHHLNHAGYYPGAQQLHLMATFAPDGTLLGAQGVGRE
                     GVDKRIDVLATALRAGFGADDLAELELAYAPPFGSAKDPVNMLGFMMQNYVSGNLKLW
                     HAEDVEWARENALILDVRSAGEVERGHLPEAVHIPHDQIRENLDRIRELADGRPVRVH
                     CESGFRSYLAHRVLAQSGFDSATFDGGMLTMQTSLPALELVNGPATLLVHA"
     gene            1809..2381
                     /locus_tag="VFC06_06020"
     CDS             1809..2381
                     /locus_tag="VFC06_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81474.1"
                     /translation="MTQFQRASWLAVLLVTLVAFGLIIPRLPAMGDESMTLTADTEVG
                     MATAAVTIPAGWDLDIAASSQQMPVASRDGVQVTTTDAVWLGETSDLLANVADLLFDG
                     DAVIPDVAAKADASPDGASSREVWRLNPSEAAGQDAPVRVDVVRDGQGVVLVVARGTA
                     NDVTALSDAIDAISASVELDLSGLDVQAST"
     gene            2378..>2638
                     /locus_tag="VFC06_06025"
     CDS             2378..>2638
                     /locus_tag="VFC06_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81475.1"
                     /translation="MRELTRTRFALNWRAPRRAGWWVLIVASAVGAYWVWQGLAPGWR
                     DFPTVGVTALVLTVPLAVAWWWLLRLPQLWARIATSGAIAAIT"
BASE COUNT          382 a          873 c          939 g          444 t
ORIGIN      
        1 ggccgtcacg tcttatgcgt gaacgagcaa ggtggctggc ccgttcacca attccaatgc
       61 gggcaacgac gtttgcatcg tgagcatgcc accgtcgaag gtcgcggagt cgaaaccgga
      121 ctgcgcgagt acccggtgtg ccaggtacga gcggaagccc gactcgcagt gcacgcgcac
      181 gggacgaccg tccgctagct cgcggatgcg gtctaggttc tcgcggatct ggtcgtgcgg
      241 gatgtgcacg gcttcaggaa ggtggccgcg ctcgacctcg ccggccgagc gcacgtccag
      301 gatgagcgca ttctcgcgag cccactccac gtcttccgcg tgccacagct tcaggttgcc
      361 ggacacgtag ttctgcatca tgaagcccag catgttgacc gggtctttcg cggagccgaa
      421 aggcggcgcg taggcgagtt caagctcggc aaggtcgtcg gcgccaaagc cggcgcgtag
      481 cgccgtcgcg agcacgtcga tgcgcttgtc gactccctcg cggcccacac cctgggcgcc
      541 aagcagcgtg ccgtccggcg caaacgtcgc catcaggtgg agctgttggg cgcccgggta
      601 gtagcccgcg tggttgaggt ggtgagtgtg gtggaccacg tagtccatgc ccgcttggtc
      661 cagcatgcgg gggctcgcgc cggtgaccgc tgcggtcagc ccgaagacgc ggacgacggc
      721 ggtgccgagc acgggcttgg catccttggc cacgccgtag atcgattcgg ccgcgcggcg
      781 gccctgacgg ttggccgggc ctgcgagcgg cacgggtgcg accacgccgg tgacgccgtg
      841 gcgcaccgcg atggcgtcgc cgacggccca gatgtgaggg tccaccgtgc gctgctgcga
      901 gtccacaatg atggcgccgc ggtcgtcgac ctcgaggccg gcgtcggatg ccaacttcga
      961 gttggggcgc acgcccacgg agaccaccac gaggtctacg gttgctgccg cgccgttggt
     1021 cagggtgacg gtcactccgc gctcggcggg ctcgatcgcg gccgcgccgg aaccggtaat
     1081 gacgtccacg ccgttggcgg tgagctcgag ttctacgagg cgcgccagtt cgggtgacag
     1141 cggcgggagc acctgtgggg caagttcgat gagcgcgacg tcgaggccgc gttcgcggag
     1201 cgcctcggcc gtctcgagcc cgatgaagcc ggcccctagc accacggcgc tgcgcgcgtc
     1261 ggtcactcgt tcggccagcg cgatcgcgtc gtcgaccgtg cgcaagtggg tgacctgggg
     1321 aaggtcgacg ccggggatgg ggggcatgaa cgcgttggcg ccgggtgaca ggacaagggc
     1381 gtcgtacgcc accttctgct caccttgcgg gcccacgacc gtcacggtct gggcggcgcg
     1441 gtcgatggtg gtgacctcgg tgttggggcg cacgtcgagg ccgagcgacg accgcagcgt
     1501 ctcgggcgtg tgcaacagca acgcctcctg ggaggtgatc tcgccgccca cgtgatacgg
     1561 cagtccacag ttggcgaagg acacgtaggg gcccttttcg aacacgatga tctcggcggt
     1621 ctcatcgagg cgacgggcgc gggccgcggc ggacattcct ccggccactc cgcccacaat
     1681 gacgatacgc atgggttctc cttgggttgt cgatgccggg ctcggtgggg tgccgtcggc
     1741 gacttctccc aagataacac ataccccctg gggtataatt cccgaaggtc ccgcgttagt
     1801 ctctgattgt gacccagttc cagcgtgcct cgtggctcgc cgtcttgcta gtgaccttgg
     1861 tcgcgtttgg cctgatcatt ccccgattgc cagcgatggg cgacgaatcg atgacgctca
     1921 ccgccgacac ggaggtgggt atggcgaccg ccgccgtgac cattcccgct gggtgggacc
     1981 tggacatcgc cgcttcgtcg cagcagatgc cggtggcgtc ccgcgacggc gtccaggtca
     2041 ccaccacgga cgccgtgtgg ctgggcgaga cctccgacct gctcgccaac gtcgccgact
     2101 tgctcttcga cggcgacgcc gtgatccctg acgttgccgc gaaggccgac gcttcgccag
     2161 acggcgcctc ctcgcgtgag gtgtggcgac tgaaccccag tgaggcggcg ggccaagacg
     2221 ccccggtcag ggtcgacgtc gtccgcgacg gtcagggcgt ggtgctggtc gtcgcgcgcg
     2281 gtaccgccaa cgacgtgacc gcactgtccg acgccatcga cgcgatctcc gcctccgtgg
     2341 agctcgacct ctcaggactc gatgtgcagg ccagcacatg agggagctga cacgcactcg
     2401 cttcgcgctg aactggcggg ccccgcgccg cgctgggtgg tgggtgctga tcgtcgcatc
     2461 ggccgtgggc gcctactggg tgtggcaggg cctcgcgccc gggtggcggg acttcccgac
     2521 cgtaggcgtg actgcgctcg tgctcaccgt ccccctcgcc gtcgcgtggt ggtggctgct
     2581 ccgcctacct caactgtggg cccgcatcgc tacatcgggg gcgatcgcgg ccatcacc
//
LOCUS       DASRKH010000376         2621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_56110, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000376 DASRKH010000000
VERSION     DASRKH010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56110"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(395..838)
                     /locus_tag="VFC06_06030"
     CDS             complement(395..838)
                     /locus_tag="VFC06_06030"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HZL81476.1"
                     /translation="MRAHDTRRAVRAASRTLLAGTLLGILVLTLVGSSGAGGVNLIPF
                     RGIIDELANVNSRLGALNLAGNVLMYMPIGLLAPLALRWGVGRVTASAFALSVTIECA
                     QLALGRSSDVDDVILNTIGALVGAVFACSFISLVRRRASRAGLEP"
     gene            complement(1095..1736)
                     /locus_tag="VFC06_06035"
     CDS             complement(1095..1736)
                     /locus_tag="VFC06_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81477.1"
                     /translation="MLELLGGSLGAVLLMGLAAVVIGFSKIALGGPAVISVAIFATIL
                     PAKESTAALLALLIVGDLAAVWHYRHDADWFLIRRLLPAAPQGSCSGRSSSGSCPTTS
                     LGRCWEEGLPRIETWTYSFRAYRLSRVLPVRHGKTAWHRLLPDPAQRLAGFHPQLRCK
                     RATHRIVHRTGVRRRERGGARRVQVFESWLTQQTRTEFRATSPVPPSSLTQPT"
     gene            complement(1859..2389)
                     /locus_tag="VFC06_06040"
     CDS             complement(1859..2389)
                     /locus_tag="VFC06_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81478.1"
                     /translation="MRIALRVILAAGIAGAYAFADGYLSLRADALRAGVDLGWHNDPS
                     IVSIVGPHFPVLYSSLLAVLSALCAYYILWRLLRGGRSGYAEAAAFVAVLLAVVVWGI
                     VTYENPGGALQTLDLSARLQWRYGVLSPALHTVMVLPVVVAIGQAVGTAFGKRTPRRV
                     GQDDAPEGNPYAPPLG"
BASE COUNT          486 a          828 c          898 g          409 t
ORIGIN      
        1 cggcggttcc gtccgcgcct cgagcgccct ggcgaccctg ggcgtggacg gggttgacgg
       61 gctgttcgtc ggccgcgccg cgtgggacgt gcatgggtac atcgaacttc tcgacctcgt
      121 ggcgagtcgc tgagcgcgtc cgacgcgggc ggttgcacac tcggtgacca ggagtgtcgt
      181 tcggttgctc cgcgtgttta caccacgcca cgagatacgt ccgactcggt tgtccggaaa
      241 gcggggtcgg ctccaacacc cgcaacgcca ccatcgtgca acgcgtctag accatcgcgc
      301 ccgacgcgac gcctggcgca caccgggtgg attagcggca agccgggttg cctgctccgg
      361 tgaagagccg ggctgaggtg gggcttggac gccgctaggg ttcaagcccc gcgcgcgacg
      421 ctcgccgccg aacgagggag atgaaggagc acgcgaacac tgctccgacg agtgccccga
      481 tcgtattgag aatgacgtca tccacatcgc tacttcggcc aagcgcaagt tgggcgcact
      541 cgatcgtgac cgaaagcgca aacgcagaag ccgtcacgcg tccgacgccc caccgcaacg
      601 cgagcggagc gagtagcccg atcggcatgt acatcagcac attgcccgca agattcagcg
      661 cgccgagtcg agagttgaca ttcgccagtt cgtcgatgat tccgcggaat ggaatgaggt
      721 tgacaccgcc cgcgccgctg gagccgacaa gggtgagcac caagatcccc aggagtgtcc
      781 ccgcgagcag cgtccttgac gcagccctca cggcacgacg ggtgtcgtgg gcacgcattc
      841 caggaaacgc gaagcccgcc gccaagacac ttgcgagtac aacaagcaca actaacaatt
      901 gcaccggcgc gtacctcagg acctccaccc acggattatt gcaactgtgg tcggcggtcg
      961 accaccggcg cgcaggggtc gtaccgggtg caccttgcgt ccgagaccgt gagccgcccg
     1021 ggctcctatg cagccggtga ccagtgtgtt cgcgagttcc tcagccgtgg acgtcaagtg
     1081 gacgatgtag tcgctcaggt cggttgcgtc agggaactgg ggggtaccgg cgaggtggcg
     1141 cggaactccg ttctcgtctg ttgtgtcaac catgattcaa acacttgcac tcgtctcgcc
     1201 ccgccgcgtt cgcgtcgacg tactccggtg cggtgcacga ttcgatgcgt ggcgcgtttg
     1261 cagcggagtt gtgggtggaa tccggcgagg cgttgcgctg ggtcggggag tagccggtgc
     1321 caggcagttt tcccatgcct gaccgggagg actcgggaga gccggtaggc gcggaatgag
     1381 taagtccacg tttcgatccg tgggagtcct tcttcccaac acctcccgag ggacgtcgtc
     1441 ggacacgacc cggaggaaga ccgacccgag cacgagccct gcggcgctgc cgggagcagt
     1501 cgccggatca ggaaccagtc ggcgtcgtgg cggtagtgcc acaccgcggc gaggtcgccc
     1561 acgatgagga gcgcgaggag ggcggcagtg gattccttgg cgggcagaat cgtcgcgaag
     1621 atggcgacgg agatgacggc ggggccgccc agggcaatct tggagaagcc gatgacgacc
     1681 gcggcgagtc ccatgaggag gacagcgccc agcgaaccgc cgagcagttc gagcatggcg
     1741 gcatccattc ttcaggttga gatgcctgga tacgcacgct agccgccgga cagatcgatc
     1801 accatctcgc ccgttgcccg cgtctcggac tcgatcgcct cgccgacgct ctggcggact
     1861 agccgagcgg aggcgcgtac gggttcccct cgggagcgtc gtcctggccg actctgcgcg
     1921 gggtccgctt tccgaacgct gtgccgaccg cctggccgat ggcgacaacg accggcagga
     1981 ccatgacggt gtggagggcg gggctgagaa cgccgtaccg ccactggagc cttgccgaga
     2041 ggtcaagcgt ctgcagggcc ccgccggggt tctcgtacgt cacgatgccc cacacgacca
     2101 ccgcgagcag caccgcgaca aacgcagcgg cctcggcgta gccgctgcgg cctccgcgaa
     2161 gcagtcgcca gaggatgtag taggcgcaca gtgcgctgag aacggcgagc agggaggagt
     2221 acagcacggg aaaatgaggg cccacgatag acacgatgga ggggtcgttg tgccatccga
     2281 gatccactcc cgcccggagg gcgtcggccc tcaaagacag gtaaccgtcg gcgaacgcat
     2341 aggcgcccgc gattcccgcc gccaggatca ctcgcaaagc aatacgcatt gttccccctc
     2401 atggtgggcg cctgggagcg ggcgccacgt tctgtctaca gggcatggct gcgaggggca
     2461 aatcagcggc cgggggaagc cgcgcccggc tgggcaccgg ccgacagtcg acgcagcctt
     2521 cgtgggtcca tgctgaaggc gtcttttcca tataccgcaa gcgattccga gaaacagaaa
     2581 gtcgagcgcg tttctctgcg taatgacgac tggtacgagg g
//
LOCUS       DASRKH010000377         2456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_17808, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000377 DASRKH010000000
VERSION     DASRKH010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17808"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1259)
                     /locus_tag="VFC06_06045"
     CDS             complement(<1..1259)
                     /locus_tag="VFC06_06045"
                     /inference="COORDINATES: protein motif:HMM:NF019935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HZL81479.1"
                     /translation="MDRSGVVRDGSHAEVPHPRGVRDLKRERQKLAVTLAVHPGPQRR
                     RALADLVCARLSEHFDAVTDGVEGLALAAVGSVGRAEAGPYSDLDLVLLHDGTSAGKA
                     AAADVAQRLWYPIWDAGLQLDHSTRSLTECRQVASKDLAAAAGLLDLRHVAGDELLAQ
                     QARSAILADWRSAARKRLPDLLAASRARAERCGELAYLIEPNIKESRGGLRDYVSLTA
                     LAATWLTDRPHGAIDDAAQRLLDVRDAVHTVSGKATHIVGRHVAQDVADALDMADTDE
                     LLASIAEAGRVIAFAVDGTERRARRSLEKSGVGSRAFLAKRRGTAPRHVPVAQGLIDV
                     DGDLALAPDYSAEADAVLPLRAAAAAASTGLTFTPSLLESFMRCPDLESPWPPEALDR
                     FTELLRNSHHLVSVWEAIDVAGQIVRWL"
     gene            complement(1276..1614)
                     /locus_tag="VFC06_06050"
     CDS             complement(1276..1614)
                     /locus_tag="VFC06_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HZL81480.1"
                     /translation="MKLVTAIIQPHRVDEVRAAIEAVGVKGVTVSEAAGYGRQKGHTE
                     VYRGAEYTVDLVPKTRLEILVEDADAAAVTDAIVSAAQTGKIGDGKVWVVPVDDVARV
                     RTGEHGADAL"
     gene            complement(1611..>2456)
                     /locus_tag="VFC06_06055"
     CDS             complement(1611..>2456)
                     /locus_tag="VFC06_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HZL81481.1"
                     /translation="VYAPIAHGVWGGGLLGTFSPSTVIDFAGGTVVHINAGAAALAFA
                     LVLGVRKGFGKEPMRPHNLPYVMLGAALLWFGWFGFNAGSEFGADATAGRAFLNTTIA
                     PAVAMLAWLLVEKIRDGKATSLGAASGIVAGLVAITPAAASVDTWGALSIGFIGGVVC
                     AYAVGLKYKFGYDDSLDVVGVHLVGGIVGTILIGFFSHLDEGTTWAPDGALNGLFYGG
                     GWEQLGSQVIGALVAVIYSLVVTLVIVYALKYTMGLRVSEDVELAGIDFAEHGETAYE
                     EANQR"
BASE COUNT          465 a          794 c          817 g          380 t
ORIGIN      
        1 agccatctga caatctgtcc agccacatcg atcgcctccc acacgctcac caggtggtga
       61 gagttgcgga gcagttcggt gaatctgtcg agcgcctcgg gcggccacgg gctttccaga
      121 tccgggcacc gcatgaatga ctcgagaaga cttggcgtga aggtgagccc cgtcgacgcg
      181 gctgccgcgg ccgctcgcaa aggaagcacc gcgtcggcct cggcggaata gtccggcgcg
      241 agggccagat cgccgtccac gtcaatgagt ccttgggcga cggggacgtg acgcggggcg
      301 gtgccacgac gcttcgcgag gaaggcgcgc gacccgacgc ccgacttctc caaggagcgg
      361 cgggcgcggc gttcggtgcc gtcgacggca aaggcgatca cacgtccggc ttccgcaatc
      421 gatgccagca gttcatccgt gtcggccatg tcgagggcgt cggcgacatc ctgggccacg
      481 tggcgaccca cgatatgagt ggcctttccc gacaccgtgt ggacggcgtc gcgcacgtcg
      541 agaaggcgct gcgctgcgtc gtcgatggcg ccgtgcggtc ggtccgtgag ccatgtcgcc
      601 gcgagcgcgg tgaggctcac gtagtcgcgc aggccacccc gcgactcctt gatgttgggt
      661 tcgatgagat acgcgagttc gccacaccgt tcggcacggg ctcgcgaagc ggcaagaagg
      721 tccggcagtc gcttgcgtgc cgcagagcgc cagtcggcga ggatcgcgga acgcgcctgt
      781 tgggcgagca gttcgtctcc agcgacatgg cgcaggtcga ggagtccggc cgcggccgcg
      841 aggtccttgg aggccacttg acggcactcg gtgagagaac gcgtcgaatg atccaactgg
      901 agaccggcat cccaaatggg ataccagagg cgttgtgcga catcggccgc ggcagccttg
      961 ccagctgagg ttccgtcatg cagaagcacc aagtcgaggt ccgaatacgg acccgcctcg
     1021 gcgcgaccca cgctgccgac cgcagcgagg gcgagtcctt cgactccgtc ggtgacggcg
     1081 tcgaagtgct ccgagaggcg cgcacagacg agatcggcaa gcgcccgcct gcgctgaggg
     1141 ccaggatgga ccgccagtgt gacggcgagt ttttgcctct cgcgcttgag gtcccgcacc
     1201 cccctgggat gcgggacctc agcgtgcgag ccgtcacgga ctacgccgga acggtccatc
     1261 gtgaatgggg tttctctaga gggcgtcggc gccgtgttcg ccagtgcgaa ctcgagcgac
     1321 gtcatccacg ggaacgaccc agaccttgcc gtcaccgatc ttgccggtct gagcggcgga
     1381 cacgatagcg tccgtcacag cagcagcatc ggcgtcctcg acgaggatct caagacgagt
     1441 cttgggaacc aggtcgacgg tgtactccgc accgcggtag acctcggtgt ggcccttctg
     1501 ccggccgtag ccggcagcct cggacacggt gacgcccttg acgcctacgg cctcaattgc
     1561 ggcacggacc tcgtccacgc ggtggggctg aatgatcgca gtgaccagtt tcatcgttgg
     1621 ttcgcctcct cgtaagcagt ttcgccgtgc tcggcgaagt caatacccgc cagttctaca
     1681 tcttcgctta cgcgcagacc catcgtgtac ttgagtgcgt agacgatgac cagtgtgacg
     1741 accaacgagt agatgacggc aacgagcgcg ccgatcacct gggagccgag ctgctcccaa
     1801 cctccgccgt agaacaggcc gttgagagcg ccatcggggg cccacgtcgt gccctcgtcc
     1861 aggtgggaga agaagccgat gagaatggta ccgacgatgc cgcccaccag gtggacgcct
     1921 acaacgtcga gcgagtcgtc gtagccgaac ttgtacttga gacccacggc gtatgcacac
     1981 accacaccgc cgatgaagcc gatggagaga gcgccccacg tgtcgaccga ggccgctgcg
     2041 ggcgtgattg caacgagacc cgcgacgatg ccggaagcgg caccgagcga agtggccttg
     2101 ccatcacgga tcttctccac cagcagccag gcgagcatgg caaccgccgg ggcaatggtg
     2161 gtgttgagga acgcacgacc tgccgtcgcg tcggccccaa actccgaacc agcgttgaag
     2221 ccaaaccagc cgaaccacag cagtgcagcg ccgagcatga cataaggcag gttgtgcgga
     2281 cgcatcggct ccttgccaaa gcccttgcgg acaccgagca ccagcgcgaa ggcgagggcc
     2341 gccgcaccgg cgttgatgtg aaccacagtt ccaccggcaa agtcaatgac cgtgctgggc
     2401 gagaaagtgc cgagcagtcc tccaccccag acaccgtgag cgatgggcgc gtagac
//
LOCUS       DASRKH010000378         2461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_11513, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000378 DASRKH010000000
VERSION     DASRKH010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11513"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..735)
                     /locus_tag="VFC06_06060"
     CDS             complement(157..735)
                     /locus_tag="VFC06_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81482.1"
                     /translation="MLNATGVTQAALAERVNVSQCTVSDWRLRRGVSSGYARAVTEAL
                     RDDSPLVLTARIAGARIDAETLRRMMNAADVSIVRIAVECGVRRKAPEHWLRCGVPAG
                     HTPHVLALMRSRGVDVDVSPLAPIEGVVLVEALRSSGATYRAFARRIGCRAETISNWS
                     RRGVPQDRIARVHASLPPEGVRVIRDAWERTG"
     gene            complement(1033..1827)
                     /gene="dut"
                     /locus_tag="VFC06_06065"
     CDS             complement(1033..1827)
                     /gene="dut"
                     /locus_tag="VFC06_06065"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HZL81483.1"
                     /translation="MTDRAVQYNGRNHAAVFDAFDTEAHGSLSRDDVAMRQIVWDMFG
                     VTPGQWLTRHADATVTVNDEEPQEARVNATPNPERPTIRIRMIDGDLPVPKFAHVGDA
                     GADLVTREGGKILPGQRLSVPTGVAIELPIGTAAFVHPRSGLALRDGITVLNSPGTID
                     SAYRGEICVILHNTDAFKPFRFSRGDRIAQLVVQQVLSPEFEVVEGLSDTARGTGGFG
                     STGVSGVATRPGSGQAFRAAMSTVTTDARPGAGQTFAAAAARGESG"
     gene            complement(1824..2348)
                     /locus_tag="VFC06_06070"
     CDS             complement(1824..2348)
                     /locus_tag="VFC06_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81484.1"
                     /translation="MSAQMLEHDVVHEMDDGGVMGFDLHGHIDWLDVETFLTEMPFDY
                     DEDGEGDFDVAWVWRWRVPGEDGSMYVYSATKAPGATPVTQILRASSWGKYCVNHPLE
                     PATQDVPAATVFEGERIVAEELAAQALRIDPRPVVDALNGGCVYLCRECSESFNERRC
                     AALRDAMANMEAAS"
BASE COUNT          413 a          871 c          766 g          411 t
ORIGIN      
        1 tacacgcgct gccgccggag acatcggacc cagcccgtgc accggacaat tggcggtcgc
       61 tgccagagtc ttgtgcccgg cgaccggcgc gctgcatctg ctcatggtct caaggtatcc
      121 acgtgcccgc gggttcggga cggggacgac tcggactcac cccgtgcgct cccatgcgtc
      181 gcgtatcacc cgcacgccct ccggcggcag tgacgcatgc actcgtgcga tgcggtcctg
      241 cgggacgccc cgccgagacc agttcgagat ggtttcagcg cggcacccaa tgcgacgcgc
      301 aaacgcccgg taggtcgccc ctgacgagcg caacgcctcc accagcacga cgccctcgat
      361 cggagccaac ggcgagacgt ccacgtcgac cccacgcgag cgcatcagcg ccagcacatg
      421 cggcgtgtga ccggcaggga cgccgcaccg gagccagtgc tcgggtgctt tgcgacgcac
      481 accgcattcc acggcgatgc ggacaatgct cacgtcggct gcgttcatca ttcgcctcag
      541 cgtctccgcg tcgatcctcg ctccggctat gcgcgcagtc agcaccagcg gagagtcatc
      601 acggagcgct tcggtcaccg cacgggcata accggacgag acgccgcgcc tgagacgcca
      661 gtcgctgacg gtgcactgcg aaacgttgac gcgttccgcc agtgcggcct gcgtgactcc
      721 cgttgcgttc agcatcgcag tgaactcgtc tcgcgggatg gcccgacgat cccccttgac
      781 cccgtagata cgggcggtgg cgggcgggcg gcggtcgcgt tctgtgccct agagcgccac
      841 cagcaccgcc ctgacttgcg cgagtcgagc gctaggcacg ccccgcacgg tccaacgcga
      901 caccgtgttc tgcctcacct ccaacgccgc agcgaccttc gactgcggaa ggtcgcgcgc
      961 aagcatctcg gagcggaacc actcagcgga atgggctgct ccgtattctc tggaccgttg
     1021 ctccacgagg gactacccgg actcgcccct ggcagcagca gccgcgaagg tctgtccagc
     1081 gccagggcgc gcgtcggtgg tcactgtgga cattgccgca cggaatgcct gcccgctgcc
     1141 aggacgcgtt gccacgccgc tcacgcccgt tgacccaaat ccgcccgtgc cacgagccgt
     1201 gtcggacaac ccctctacaa cctcgaactc cgggctcaac acttgctgca ccaccagttg
     1261 ggcgatgcgg tctccgcgac tgaaccggaa gggcttgaac gcgtcggtgt tgtgcaggat
     1321 cacgcagatt tcgccacggt atgccgagtc gatcgtgccg ggcgagttga gcaccgtgat
     1381 gccgtcacgc agcgcgaggc cggagcgcgg atggacgaac gcagcggtcc cgataggcag
     1441 ttcaatggcg acaccagtag ggacagagag tcgttgcccg gggagaatct tcccgccttc
     1501 tcgggtcact aggtccgccc cggcgtcacc cacgtgggcg aacttcggga cgggcaggtc
     1561 gccgtcgatc atgcggatgc ggattgttgg ccgctcgggg ttcggggttg cattcacacg
     1621 ggcctcctgc ggctcctcgt cgttcactgt caccgtggcg tcggcgtgcc gcgtcagcca
     1681 ctgtccgggc gtgacgccaa acatgtccca cacgatctgg cgcatagcga catcgtcgcg
     1741 actcagcgag ccgtgagcct cggtgtcgaa ggcatcaaac acggcggcgt gattcctccc
     1801 gttgtactgc acggcgcggt ccgtcatgat gctgcctcca tgttggccat ggcgtcgcgc
     1861 agggccgcgc aacgacgttc gttgaaagat tcggagcatt cccggcacag gtagacacac
     1921 ccgccgttga gcgcgtccac gaccggcctc gggtcaatgc gcaacgcctg ggcggcaagt
     1981 tcctctgcga cgatccgctc tccctcgaac acggtcgcgg ccggaacgtc ttgcgtcgcc
     2041 ggctcgagcg ggtggttgac gcagtacttg ccccaactcg acgcccgcag aatctgtgtc
     2101 accggggtcg cgcccggagc cttggtggcc gagtagacgt acatcgaacc gtcctcgcca
     2161 gggacccgcc agcgccacac ccacgccacg tcgaagtcac cctcgccgtc ctcgtcgtag
     2221 tcgaagggca tctccgtcag gaacgtctcc acgtcgagcc agtcgatgtg gccgtgaagg
     2281 tcgaaaccca tcacgccgcc gtcgtccatc tcgtgcacca cgtcatgttc aagcatctgt
     2341 gcgctcatcg gaaagcacct ccacattctt cacggtcgcc gcttcgcatc gccaagcgtc
     2401 ggtattcggt ccatgctcgg gatcacgttc gaccacgccg agcggctcga cagtcaggac
     2461 t
//
LOCUS       DASRKH010000379         2056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_65087, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000379 DASRKH010000000
VERSION     DASRKH010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65087"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(314..1081)
                     /locus_tag="VFC06_06075"
     CDS             complement(314..1081)
                     /locus_tag="VFC06_06075"
                     /inference="COORDINATES: protein motif:HMM:NF019151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1524 domain-containing protein"
                     /protein_id="HZL81485.1"
                     /translation="MAALTVKGRAPKTGYDRDQFGTAWTDVDRNGCDTRNDMLGLRLT
                     NRDMSGTCKVLSGDLDDPYTGTWIHFEIGGASEVDIDHIVALSDAWQKGAFAWEFAQR
                     VALANDPLNLEPVEASANRQKGDSDAASWLPSNKSFRCQYVARQAAVKTKYDLWVTQA
                     EQDAMLRVLEACPGEPMPAPGAQPILADNTGGPPPAPAETSAPAPVEVPAAVGDTDPD
                     WGTCTEAKKHGGGPYVQGVDAEYTFYRDGDKDGTVCE"
     gene            complement(1309..>2056)
                     /locus_tag="VFC06_06080"
     CDS             complement(1309..>2056)
                     /locus_tag="VFC06_06080"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HZL81486.1"
                     /translation="EDHSFVADGFVVHNCQGHSPASRPNRRAFHEAVRATAFAVIDCA
                     DVTEPEAIIVENVPSFLRWRLYPWWREGLERLGYEVENRIVTASHMGVAQRRTRLFVI
                     ATRPGVVVRPFTLSADEPAFGQHIHREVDERLWRFVSAATPGVQERIHRGRSRYGDRF
                     LTQHVTGHPGVSLAEPIRTITTASQHWNLVEGDRYRALTTRELARGMGFPDTYTWDDA
                     LSNAAVTQGIGNAVCPPVAREVVSAVAEAL"
BASE COUNT          317 a          681 c          702 g          356 t
ORIGIN      
        1 actcttgtgg ctcgccaccg tctctcgcag ccgcgagtac ttcacttcgg caagtcgctc
       61 caacgcctcg ggcggggtcg cggggtcgcg agccaaggtg atcctcttgg cgtagtcctt
      121 gtcgtcttcg cgcagcacag attcccaggc gggctcctgc cctgctggag ccgatggggc
      181 cggaggcgca ctcggctcac cgtgcaggcg agacactcca tgcacggggc acgcggcagc
      241 agagcctggc ttgcggtggc cttccaccgg tgcggtgcat gtcctcatgc gcggaaggta
      301 tccaggcagc gcactactcg cagacggtcc cgtctttgtc gccgtcccgg tagaaggtgt
      361 actccgcgtc gacgccctgc acgtatgggc ctccgccgtg cttcttcgcc tcggtgcatg
      421 tcccccagtc agggtcagtg tcaccgacgg ctgcgggcac ctcgacgggt gcgggtgcag
      481 acgtctcggc aggcgcaggc ggcgggccgc ccgtgttgtc ggcgaggatt ggttgcgcgc
      541 cgggtgcagg catcggctcg ccggggcacg cttcgaggac gcgcagcatt gcgtcctgtt
      601 cggcctgcgt gacccacagg tcgtacttgg tcttcacggc ggcttgccgg gcgacgtact
      661 ggcagcggaa cgacttgttg gacggcaacc aggacgcagc gtccgagtca cccttctgac
      721 ggttggccga cgcctcgacg ggctccaagt tcaacgggtc gttcgccagg gcgacacgct
      781 gcgcgaactc ccacgcgaag gcacccttct gccaggcgtc ggacagggcg acgatgtggt
      841 cgatgtccac ctcgctagcg ccaccgatct cgaagtgaat ccacgttccc gtgtacgggt
      901 catcgaggtc cccggacagc accttgcagg tgcccgacat gtccctgttc gtcagacgca
      961 agccgagcat gtcattgcgc gtatcgcacc cgttgcggtc cacatcggtc cacgcggtgc
     1021 cgaactgatc gcggtcgtag ccggtcttcg gtgcgcggcc cttgacggtc aacgcggcca
     1081 ctgcatccgc ggcggtgccg ctcaacgcag caggtgatgc aacgggcgtc tcggagacct
     1141 ctacgtcggg cgttggggaa gtagattgcc cgcccacggg taccacaggg cttgtgggcg
     1201 cctctggcgg cgtgacgggg ctcgttgtgg ccacggacgg cccacaggct gttagagagg
     1261 cgcacagcgt tgcggcgata agagcggcgg ctgcgcgccg ggtcggactc atagcgcttc
     1321 cgccacggca gagaccactt cacgggccac cggcgggcac acagcgttgc cgatgccctg
     1381 tgtgacggcg gcgttcgaga gtgcgtcgtc ccacgtgtac gtgtccggga accccatgcc
     1441 tcgtgcgagt tccctcgtcg tcagcgcccg gtagcggtcg ccctccacga ggttccagtg
     1501 ctggctcgcc gtggtgatcg tgcgaatcgg ctccgcgagg gagacgcccg gatgtccggt
     1561 gacgtgctgc gtgaggaacc tgtcgccgta ccgggaacgg ccacggtgaa tccgctcctg
     1621 cacgccaggt gtggctgccg agacgaaacg ccacagacgc tcatcgacct cacggtgaat
     1681 gtgctgtccg aacgcgggct cgtcggccga gagcgtgaag ggccgcacca ctacgccggg
     1741 acgtgtggcg atcacgaaca gccgggtgcg cctctgtgcg acgcccatgt gtgaggcggt
     1801 cacgatgcgg ttctccacct cgtagccgag acgttcgagt ccctcacgcc accacgggta
     1861 gagacgccac cgcaggaacg acggaacgtt ctccacgatg atcgcttcgg gctccgtgac
     1921 gtccgcgcag tcgatcacgg cgaacgcggt agcccgcacg gcttcatgga aagcgcgacg
     1981 gttaggtcgc gaggccgggg agtgcccctg gcagttgtga accacgaagc catcagcgac
     2041 gaacgaatgg tcctcc
//
LOCUS       DASRKH010000380         3699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_47135, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000380 DASRKH010000000
VERSION     DASRKH010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47135"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="VFC06_06085"
     CDS             complement(<1..896)
                     /locus_tag="VFC06_06085"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HZL81487.1"
                     /translation="MTKREIDLGGVVVATTLAFKEDASAPAGQAVDFDRFAEHCDFLM
                     NNGCRGVGPNGSLGEYEALTDEERRKVIQVAVKAVAGRGIVIAGAHGTGWHTAKKWAE
                     YAKEDGADGLLLLPPTIYKANDDEIVEHYAKVAEVGLPIMVYNNPFSTKHDLSPSLIA
                     RLAEIPEVVAVKEFSGDVRRVLEIKELCDIDVICGSDDLLFESLVDGADGWFAGYPNA
                     FPKEAVEIYDQVKAGNIQAALDLYTPLAAVFRWDSKIEFVQAIKLSIDLAGQSYGGVT
                     RPPRGPLSAEHHAKVTADTKKAL"
     gene            complement(889..2325)
                     /locus_tag="VFC06_06090"
     CDS             complement(889..2325)
                     /locus_tag="VFC06_06090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020018268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HZL81488.1"
                     /translation="MREVLVIGAGPAGLAAARAARDAGASVVLLDSSDALGGQFWRHL
                     PSTRPSRNEKLLHHGWSRFHRLSDALESDARVRILRSAQVWAIEVLDGVAVHAIIGEP
                     DGRQRERRVFRPDALVLATGAHDRTLPFPGWDLPGVFTGGAAQALAKGERLAVGERVL
                     VAGAGPFLLPVAQSLTLAGSRVLAVLEANHAARLIRGWSPRPWQLLGAAGKGVELAGY
                     VAGQVRHRIPYRLGRAVTAAHGTDRVESVTTARLDAHWAPVAGTEETVEVDAICVSHG
                     FTPRIELAIAAGCALTRERFVVVDEHQRTSIDGVYAAGEITGIGGADLALAEGEIAGL
                     SAAGGTARSGTARSGPARRRRIFTGFANRIEVAHGIQPGWTEWLDDATVICRCEEVDC
                     GSLRTVARSTQSESFRALKLSSRAGLGICQGRMCGRTVEHLLCTFSENVPLKDDAHSD
                     RRPIALPLRLSELAEPTSHKDQRKGNHD"
     gene            complement(2322..2678)
                     /locus_tag="VFC06_06095"
     CDS             complement(2322..2678)
                     /locus_tag="VFC06_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZL81489.1"
                     /translation="MAARMVPSHEDPIRPDDPITLSITVDGEVITGLQGQSVAGVIMA
                     NDRLGFRQTAKRDRPRGVFCGIGVCFDCLVEVNGSSDVRACQRKAGDGDMVVTQGNSG
                     PQDPELHGSELHEDVS"
     gene            complement(2678..>3699)
                     /locus_tag="VFC06_06100"
     CDS             complement(2678..>3699)
                     /locus_tag="VFC06_06100"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZL81490.1"
                     /translation="GAELVMALAAAARWPRLAEELSEELGPQFPSIEFEAKGGLVVST
                     TEDGAGPLLAFASAQRASGVEAQELTVAEALGLEPDLNPAITAAVHYRQDSQVQPVNA
                     AEALLASARKHGARVLQNTRITAPILNADGALVGIATDRGELRADAVVIAAGPWSGEV
                     ALTLGVVLPVLPRHGMVLVTSRMTKRIFHKVYDADYVGAVGSGDRVLQTSSVVESTES
                     GNVLIGSSREQIGFDNRLRVDTVSQIAEKALRLFPFLADQAIIRTYGGFRPFMPDHLP
                     LVSQDHRLPGLWHATGHEGAGIGLAPVTGELLTAMVTGATPPLDPAPYLLSRESLAPH
                     LVAVN"
BASE COUNT          679 a         1236 c         1142 g          642 t
ORIGIN      
        1 agggccttct tagtgtcagc ggtcaccttc gcgtggtgct cagcggaaag cgggccgcgc
       61 ggcggacggg tgacgccgcc atagctttgg ccagccaggt caatcgacag tttgatggcc
      121 tgcacgaact cgatcttcga gtcccagcgg aacacagcgg cgagtggggt gtagaggtcg
      181 agagcagcct ggatgtttcc ggccttcacc tggtcgtaga tctccactgc ttctttcggg
      241 aacgcgttcg gatagccggc gaaccaaccg tccgcgccgt ccacaagcga ctcaaacagg
      301 aggtcgtcgg agccgcagat cacgtcaatg tcgcacagtt ccttgatttc caagaccctc
      361 cgcacgtcac cagagaactc cttgaccgcg acaacttcgg gaatctcagc aagcctggca
      421 atcagactcg gtgacaggtc gtgcttggtg gagaacgggt tgttgtagac catgatcggc
      481 aggccaacct cggccacctt cgcatagtgc tcgacgattt catcgtcgtt cgccttgtag
      541 atggtcggtg gcagcaaaag caggccatcg gcgccgtctt ccttcgcgta ttctgcccac
      601 ttctttgcag tgtgccatcc ggtaccgtgc gcaccagcga tgacgattcc gcgcccagca
      661 accgccttca cggcaacctg gatgaccttg cgtcgttcct cgtcggtgag cgcctcgtac
      721 tcgccgaggg acccgttcgg gccgacgccg cggcatccgt tgttcatcag gaaatcgcag
      781 tgctctgcga acctgtcgaa gtcgacggcc tggcccgctg gcgcagaggc gtcctctttg
      841 aacgccagag tggtggcgac aacgacgcct ccgaggtcga tttcccgctt agtcatggtt
      901 tccctttcgt tgatctttat ggcttgtcgg ttcagcgagt tcactgagcc gaagtgggag
      961 agctatgggt cgacggtcag agtgcgcgtc atccttgagt gggacgttct ccgaaaacgt
     1021 gcacagcaag tgttcaacgg tgcgcccgca catccggccc tgacatatcc caagcccggc
     1081 gcggctactc aacttcagtg cgcggaagga ctccgactgt gtgctgcgcg ccaccgtgcg
     1141 cagggagccg cagtccacct cttcgcagcg gcagatcacc gttgcatcgt cgagccattc
     1201 agtccagcct ggctggatgc cgtgagccac ctcgatgcgg ttcgcgaagc cggtaaagat
     1261 gcggcggcgg cgggcagggc cggaacgggc agtgccggaa cgggcagtgc cgcccgctgc
     1321 gctgagaccc gcaatttcgc cttctgccag cgccagatcc gctccaccga tcccggtgat
     1381 ctcgccggcg gcatacacac catcgatgct tgtgcgttga tgttcatcga ccaccacgaa
     1441 gcgctcgcgt gtgagcgcac agccagcagc aatcgccaac tcaatcctcg gggtgaatcc
     1501 gtggctcacg catatcgcat caacctcgac ggtttcttcg gtcccggcga cgggtgccca
     1561 gtgcgcatca agtcttgccg tggtcacgga ttcgacccgg tcagtcccgt gggccgcggt
     1621 caccgccctg ccgagccggt aagggattct gtgacgcacc tgcccagcaa catatccagc
     1681 aagctccacg cctttacccg ctgcacccaa aagttgccac ggcctcggcg accatccacg
     1741 gatcagccgc gccgcgtggt tcgcttcgag caccgcgagc acccgggaac cggccagcgt
     1801 cagtgactgc gcgaccggga gcagaaatgg tccagccccc gcgacaagca cacgctcacc
     1861 gacggcgagc cgttctccct ttgccagcgc ctgggccgca ccgccagtga acacaccggg
     1921 aagatcccag ccgggaaagg gaagggtcct gtcatgagca ccggtcgcca gcacgagcgc
     1981 atccggccgg aatacccggc gctcgcgctg cctgccatcc ggctcgccga tgatcgcgtg
     2041 aactgccaca ccgtcgagaa cctcgatcgc ccacacctgg gcgctgcgga ggatgcgcac
     2101 acgcgcgtcc gattcgaggg cgtcactcag tcggtgaaac ctcgaccaac cgtgatgcag
     2161 aagtttctcg ttgcgcgaag ggcgagtgct cggaaggtgg cgccagaatt ggccgccgag
     2221 ggcatccgag gagtccagca gcacgaccga cgccccagcg tcccgcgccg cgcgcgccgc
     2281 cgccaggccc gcaggaccgg caccgatcac cagcacctct ctcatgagac atcctcgtgc
     2341 aattcagagc cgtgcaactc aggatcctgg ggcccagaat tcccctgggt gaccaccatg
     2401 tcgccgtcac cggccttccg ttgacacgcg cggacgtcgc tcgacccatt cacctccacc
     2461 aggcagtcga agcagacccc aatgccgcag aacaccccgc gcggccgatc acgtttcgcg
     2521 gtctgccgga aacccagccg gtcgttcgcc atgatgacac cggcaacgct ctggccctgc
     2581 agtcccgtga tcacctcacc gtcgaccgtg atcgagagtg ttattgggtc gtccggtcgg
     2641 atcggatcct cgtggctcgg aaccatgcgc gcggccacta gttgaccgcc acgaggtgcg
     2701 gtgcaaggga ctcccgcgac aagagatacg gtgctgggtc aagcggtgga gtcgccccgg
     2761 tgaccatggc ggtgagcaac tcgccagtga cgggggcaag accgattccc gcaccctcgt
     2821 ggccggtggc gtgccacagc ccaggcagac ggtgatcctg actcaccagc ggcagatgat
     2881 cgggcatgaa gggccggaat ccgccgtacg tccggatgat cgcctggtcg gcgaggaacg
     2941 ggaagagccg gagagccttt tccgcgatct gagaaaccgt gtcgacgcga agccgattgt
     3001 cgaatccgat ctgctcgcgt gaggagccaa tcagcacatt gccggattcg gtggattcga
     3061 caacactcga ggtttgcagt acccgatccc ccgagccgac agccccgacg tagtcggcgt
     3121 cgtagacctt atggaagatc cgcttcgtca tgcgggaggt gacaagcacc atgccgtggc
     3181 gcggcagaac cggcaggaca acgccgagcg tgagtgccac ctcaccggac cacggcccgg
     3241 ccgcgatgac aacggcgtcg gcacggagct caccgcggtc tgtggcgatg ccgacgagtg
     3301 caccgtctgc gttcagaatg ggtgccgtaa tgcgcgtatt ctgcagcact ctcgcgccgt
     3361 gcttgcgtgc ggatgcgagc aatgcctctg ccgcattcac cggctgaacc tgagagtcct
     3421 gccgatagtg aactgctgcg gtgattgccg ggttgaggtc gggctcgagc cctagcgctt
     3481 ccgcgacggt gagctcttgc gcttcgaccc ccgaggcccg ctgggcggag gcgaaagcca
     3541 gcaacggtcc agcgccgtcc tcggtggtcg agacaaccag gccacctttg gcctcgaact
     3601 cgatcgacgg gaactggggc cccaactcct cggacaactc ctccgccagg cgtggccagc
     3661 gggccgctgc ggcaagcgcc atcacgagtt ctgcacctg
//
LOCUS       DASRKH010000381         2341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_19064, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000381 DASRKH010000000
VERSION     DASRKH010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19064"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="VFC06_06105"
     CDS             complement(<1..564)
                     /locus_tag="VFC06_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81491.1"
                     /translation="MEQRRYRRPAMLDMEEAELIIGDIDPLDRALVAHESAAALVAQA
                     RALRDPEVTARLIRLVDEEGIDTVAMMWAKAEPHTLPGALWRIYMLREWVKFSAESVA
                     RSYHHGVQAAQVRHVIAGVEDPPGPDEVRTLADLILSGVFSGDLAVALERAGAFCRVV
                     ATGAAFEAQSDEGHADSRAHEATLRAAQ"
     gene            complement(568..1050)
                     /gene="msrB"
                     /locus_tag="VFC06_06110"
     CDS             complement(568..1050)
                     /gene="msrB"
                     /locus_tag="VFC06_06110"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005509041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HZL81492.1"
                     /translation="MSPNKIVKTDAEWRAQLGDFEYEVLREAATERPWTGELLEEKRA
                     GIYRCRACGTELFRSNTKFDSHCGWPSFFEAENDAVTLLEDRTLGMVRVEARCAACDS
                     HLGHVFPDAPQTPTGNRFCINSVSLTFDDAAPVDDAAPVDDAPVDDAPVDDAESGDAS
                     "
     gene            1393..2232
                     /locus_tag="VFC06_06115"
     CDS             1393..2232
                     /locus_tag="VFC06_06115"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HZL81493.1"
                     /translation="MTSPLPEFDSAGRANGAANGGPILGIGRLQWADAWAGLKAIAPM
                     TPGATAFGLAFGALVPAMGINPWTGVYASASIMAGASQISVFESIRTGAPAVVAILTA
                     LIINARFALYSAALGPVFSAFPRRWKLGLAHLMADPSAVVALQHADKYSDPVRKRWFV
                     MGAALPLQIIFLMGTAVGVVVGPIIPEAWQIGFIVPLMFISVMVPTLRRSEDVVALGI
                     TGAVALLSRGLPFGLNVLVGIVSGITVGTLWAEWVDARRRRRGQHVDNVIEAAEHEAE
                     GGL"
BASE COUNT          349 a          795 c          792 g          405 t
ORIGIN      
        1 ctgggcggcc cgcagcgtgg cctcgtgggc gcgggagtcg gcgtggccct cgtccgactg
       61 agcctcgaac gcggcacccg tggcgaccac tcggcagaac gccccggccc gctcgagcgc
      121 gacggccagg tccccgctga acacccccga caggatcagg tccgcaagcg tgcgcacctc
      181 gtccggtccc ggggggtcct cgactcccgc aatcacgtgg cgcacctgcg cggcctggac
      241 cccgtggtga taggaccggg ccacggactc ggccgagaac ttgacccatt cgcgcagcat
      301 gtagatgcgc cacagcgcgc cgggcagtgt gtgcggctcg gccttcgccc acatcatggc
      361 cacagtgtcg atgccctctt cgtccactaa gcgaatcagg cgtgcggtga cctcgggatc
      421 acggagcgcc cgcgcctgag cgacgagcgc cgccgcagat tcgtgggcca cgagcgcgcg
      481 atccagggga tcgatgtcgc cgatgatcag ttcggcttcc tccatgtcga gcatggcggg
      541 gcgccggtag cgtcgctgct ccaccaccta ggaggcgtcg ccggactcgg cgtcgtcaac
      601 gggtgcgtcg tcaacgggtg cgtcgtcaac gggtgcagcg tcgtcaacgg gtgcagcgtc
      661 gtcgaaggtc agcgacaccg agttgatgca gaagcggttg ccggtgggcg tctgcggcgc
      721 gtcggggaac acgtgaccca agtgcgagtc gcacgcggcg catcgtgcct cgacgcgcac
      781 catgcccagg gtgcggtcct ccagcagggt gaccgcatcg ttctcggctt cgaagaagct
      841 cggccatccg cagtgggaat caaacttggt gttcgagcgg aagagctcgg tcccgcacgc
      901 acggcagcga tagatgcctg cgcgcttctc ttccagcagc tcgccggtcc aagggcgctc
      961 ggtggcggcc tcccgcaaca cttcgtactc gaagtccccg agctgggcgc gccattcggc
     1021 gtccgtcttc acgatcttgt tgggtgacat tgccactcct tccgtgcctc acgctagcgg
     1081 gccgtgcggt gcgtctcgac gattccgtcg cgatgcgaac cgggccaggc gcggcgtcat
     1141 cggccacgcg cccgccagag tgcgagaccc acgggaagag agatggcagg cgcccaactc
     1201 cgaggggggg ttggagctgg gcgccgcact tctacatctc cccgtgaggc tcgcgccttg
     1261 gcgggtacaa cgttcccaac gatgagaacg tgtcacttgt tcccaccatt gtcccccacg
     1321 gtgaaccggt ttttgacacg aggagcctac cgacgccagg gcggaccccg acgctcatgc
     1381 gacactggct cggtgacgtc acccctaccc gagttcgact cggcgggccg agcgaatggc
     1441 gcggccaacg gcggcccgat cctcggcatc gggcgcctgc aatgggccga tgcttgggcg
     1501 gggctcaagg cgatcgcacc aatgacccca ggtgccaccg cattcggcct cgcctttggg
     1561 gcgctggtgc ccgcgatggg gatcaacccg tggacgggcg tgtacgcgtc ggcgtcgatc
     1621 atggccggcg ctagccagat ctcggtcttc gagtcgatac gcacgggcgc tcccgccgta
     1681 gtcgccatcc tcacggcact catcatcaac gcgcgcttcg cgctgtactc ggcggcgttg
     1741 ggaccggtct tctccgcgtt cccgcgccgc tggaagctgg gcctcgccca cctcatggcc
     1801 gacccatccg ccgtggtggc gctgcaacac gcggacaagt actcggatcc cgtgcgtaag
     1861 cgctggtttg tgatgggcgc ggcactgccg ctgcaaatca tttttttaat gggtaccgcg
     1921 gtaggcgtcg tcgtcggccc gatcatcccc gaggcgtggc agatcggctt catcgtgccg
     1981 ctcatgttta tttcagtgat ggtgcccacg ttgcgccgct ccgaggacgt ggtcgcgttg
     2041 ggcatcaccg gggccgtggc gctcctgtct cgcgggctgc cctttgggct caacgtgctg
     2101 gtgggcatcg tgagcggaat caccgtgggg acgctgtggg ccgagtgggt cgacgcacgg
     2161 cgacggcgcc gcgggcagca cgttgacaac gtcatcgagg ccgccgagca cgaagctgag
     2221 ggcgggctat gagcgtcttc ctgatcttca cggccgtggc cgtcgggacc tacctgattc
     2281 gcatctccgg catcgtgctc ctcagcaatc cggagaagct gcctccgcga ctgcgccgtg
     2341 c
//
LOCUS       DASRKH010000382         2540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_15799, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000382 DASRKH010000000
VERSION     DASRKH010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15799"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(626..1342)
                     /locus_tag="VFC06_06120"
     CDS             complement(626..1342)
                     /locus_tag="VFC06_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZL81494.1"
                     /translation="MVSLSLRDVVVTYPAGRGQTVAVDGVSLDVARGEVVALLGPSGC
                     GKSSLLRAVAGLEPLAGGDVLWDGQSVTGVPVHRRGFGLMFQDGQLFPHRDVAGNVAY
                     GLAGAPREEQRARVAHLLELVGLEGFGRRSVSTLSGGERQRVTLARSLAPRPRLLMLD
                     EPLSALDRSLRERLTEDVARILRASSTTSLYVTHDHAEACAVADRVGIMMAGRLVQIG
                     TPEQVWGAPASDPVAEFLGV"
     gene            complement(1336..>2540)
                     /locus_tag="VFC06_06125"
     CDS             complement(1336..>2540)
                     /locus_tag="VFC06_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZL81495.1"
                     /translation="SVVVRMVGGMWARLDPRTVEAARSLGASPARAFLTVTLPALAPA
                     VASAASVVFLFCATAFGVVLILGGIHFGTIETEIWVQTTQFLNLRAAAVLSVVQLVVG
                     AAALAVAGRARSARERSLQLRAEHHRPLDPARDAVPLAVTAVVVLGLLTLPLANLLVR
                     SFHTAAGWGLDNYRNLGTSSRALTVTVWEAMGNSLRVAVDATLLAVVLGVMVAVVVAQ
                     RPRSASGRRAVSLLDGLFMLPLGVSAVTVGFGFLITLNRPPLDLRSSMVLIPIAQAIV
                     ALPMVVRTVLPVLRAIDPRQREAAATLGAGPARVFATVDGPHLVRALGLAVGFAFAIS
                     LGEFGATSFLARPDRPTLPVVIFRLIGRPGAENFGMALAASVVLAVMTAGVMALAERL
                     RIDRTGEW"
BASE COUNT          398 a          961 c          910 g          271 t
ORIGIN      
        1 gaggtcccgg aggtcaccga aggcggcccg ttcgaagtgt tcgacggctt cgtccggcag
       61 gccggcgcgc gccgcgatga tggcctgcac ggcaggacca tgcgacggag ccattcggtg
      121 ggttcccgtg gtcctggcga ggtttcgagc cgaggaagag gcgtggaacc ctcgccttcg
      181 atccgagacc tcgccaacca ggattcgaga cctcgccccc ggttcacgac ctcgccttcg
      241 gcgcgggaat tcggcgcggc ggggcggtcc gggcaaccgg ccggcccgtt cgcttcgcct
      301 cagacccccc gtgccccatc cccacaggca cgggtcgcgt cccgaatcga tcccggaacc
      361 gtttggtggc ttcctgcggt cctgtggttg cagtcggtga ggcttcgacc ccgcggtgag
      421 gggtacaggc ctcaccaccg gttcgaactc ttaccagggc tgccgggaca cccgcggact
      481 cgcccgcagc gccgagcctc cgcgccacct cgcgcggtcg gtgaggcttt gcaccgacgg
      541 tgtggggcat atcccacacc gtgggcacga aacctcgccg cggatggatg gtgcgggttt
      601 gaactcgggc acgcggcccc acgcgtcaca ccccgaggaa ctcggccact gggtccgacg
      661 ccggcgcccc ccacacctgc tccggcgtgc cgatctgcac cagccgaccg gccatcatga
      721 tgcccacccg gtcggcgacc gcgcaggcct cggcgtggtc gtgggtgacg tagagcgacg
      781 tcgtcgacga cgcccgcagg atccgcgcca cgtcctccgt cagccgctcc cgcaggctgc
      841 ggtcgagggc ggagagcggc tcgtccagca tcaacaggcg cgggcgcggg gcgagcgagc
      901 gagcgagcgt cacccgctgc cgctccccac cggacagcgt ggacacggac cggcgcccga
      961 acccctcaag ccccacgagc tccagcaggt gcgcgacccg cgcgcgctgc tcctcgcgcg
     1021 gcgcgccggc cagcccgtag gcgacgttcc ccgccacgtc ccggtgcggg aacagctgcc
     1081 cgtcctggaa catcagcccg aagccgcggc ggtgcaccgg gacgcccgtc acgctctggc
     1141 cgtcccacag cacgtcgccg ccggcgagcg gctcaagccc ggccaccgcg cgcagcagcg
     1201 acgacttgcc gcacccactc ggcccgagca gcgccaccac ctcgccccgc gccacgtcca
     1261 ggctcacgcc atcgaccgcc acggtctgcc cgcgccccgc cgggtaggtc acgacgacgt
     1321 cgcgcaagga caggctcacc actcccccgt cctgtcgatc cggagccgct ccgcgagcgc
     1381 catgaccccg gccgtcatca ccgcgaggac cacggacgcc gcgagcgcca tcccgaagtt
     1441 ctccgcgccg ggccggccga tgagccggaa gatgaccacg ggcagggtgg ggcggtcggg
     1501 ccgcgcgagg aacgacgtcg ccccgaactc ccccagcgag atcgcgaacg cgaagccgac
     1561 ggccaggccg agcgcacgga cgaggtgggg tccgtccacg gtggcgaaga cccgggcggg
     1621 gccggcgccg agcgtggcgg ccgcctcccg ctggcgcggg tcgatcgcgc gcagcacggg
     1681 gagaacggtc cgcaccacca tcggcagggc cacgatcgcc tgggcgatcg ggatgaggac
     1741 catcgaggag cgcaggtcca gcggcggccg gttgagggtg atcaggaagc cgaaaccgac
     1801 ggtcaccgcc gagacaccca acggcagcat gaacaggccg tccaggagtg agacagcgcg
     1861 ccgcccactg gccgagcggg ggcgctgggc caccaccacg gccaccatca ccccgagcac
     1921 caccgccagc agggtggcgt ccacggccac gcgcagcgag ttgcccatgg cctcccacac
     1981 ggtcaccgtc agggcgcggg acgaggtccc gaggttgcgg tagttgtcca ggccccagcc
     2041 ggcggcggtg tggaaggacc gcaccagcag gttcgcgagc gggagagtga gcagcccgag
     2101 cacgacgacg gccgtcaccg ccagcggcac cgcgtcgcgc gcggggtcca ggggccggtg
     2161 gtgctccgcg cgcaactgga gcgaccgctc ccgcgcggag cgggcccggc cggcaacggc
     2221 gagggccgcg gcccccacga cgagctggac caccgagagc accgcggcgg cgcgcaggtt
     2281 caggaactgg gtggtctgca cccagatctc cgtctcgatg gtgccgaagt ggatgccgcc
     2341 gaggatgagg accacgccga acgccgtcgc gcagaagagg aacaccacgg acgcggccga
     2401 ggccaccgcg ggtgccagcg cggggagggt cacggtgagg aaggcgcgcg ccggcgaggc
     2461 gccgagggag cgggcggcct ccacggtccg ggggtcgagc cgcgcccaca tgccgccgac
     2521 catccggacc accacggagt
//
LOCUS       DASRKH010000383         3039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_46375, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000383 DASRKH010000000
VERSION     DASRKH010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46375"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="VFC06_06130"
     CDS             complement(<1..694)
                     /locus_tag="VFC06_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HZL81496.1"
                     /translation="MSSDATTGPGFRIEHDTMGEVRVPADALYRAQTQRAVENFPISG
                     TRLERSHIEALARIKKAAALANAELGVLDANLAAAIVAAADEVAAGLHDRDFPIDVFQ
                     TGSGTSSNMNANEVIATLAAARLGREVHPNDHVNASQSSNDVFPTSVHVAATAGVVRD
                     LIPALEHLAGTLAAKSTEFADVVKSGRTHLMDATPVTLGQEFGGYAAAIRYGVERLQT
                     ALPRAAEVPLGGT"
     gene            complement(790..1416)
                     /locus_tag="VFC06_06135"
     CDS             complement(790..1416)
                     /locus_tag="VFC06_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HZL81497.1"
                     /translation="MTPAEAWAALRDGNDRFVRGAMEHPSQGIDHRASLSREQHPFAV
                     IFGCSDSRVAAEIIFDQGLGDVFVVRTAGHVLDTTVIGSIEYGVELLDASLVVVLGHD
                     SCGAVAAATTALTTGELPRGFVRAIVDRVIPSIVNIAGGEDRRALASLSAAELGHEHV
                     RHTVSMLQGYSAALAAAVDEGRCAIVGLEYTLADGRVHIAETLGDIGA"
     gene            complement(1425..2381)
                     /locus_tag="VFC06_06140"
     CDS             complement(1425..2381)
                     /locus_tag="VFC06_06140"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015771035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HZL81498.1"
                     /translation="MTRDDAAPGALVPRALVVGEALIDVVRRTDGSVAEHPGGSPMNV
                     AIGLGRLGRGVDLLTWLGQDARGDAVRRHLEASAVRVLTGDTQPASTSVATATLDADG
                     VATYDFDLTWDLPARWDAPVVDPLVVHTGSIAAVLAPGGAAVAQLVAAHRASSTITYD
                     PNLRPALMGTPGATRPRVEALVALSDVVKVSDEDLEWLHPGVAPTDIAEEWARLGPGL
                     VVVTHGGDGAFACTATGVKLTVPAPRVEVADTVGAGDSFMSGLIDGLWSAGLLGADRR
                     GALADVDAATLQRVLDRCARIAAITVSRAGANPPSSVELGEA"
     gene            complement(2378..2629)
                     /locus_tag="VFC06_06145"
     CDS             complement(2378..2629)
                     /locus_tag="VFC06_06145"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019134993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HZL81499.1"
                     /translation="MDLTGLSYEQARDELVAVVARLEAGGASLEESLELWERGEALAA
                     RCQEWLDGARRRLESSRGDGSPTSTEDAADAAQQSGGVR"
     gene            complement(2774..>3039)
                     /locus_tag="VFC06_06150"
     CDS             complement(2774..>3039)
                     /locus_tag="VFC06_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HZL81500.1"
                     /translation="GPERDVRRLRAAAGSALDRALTLAAGQCGRLAAQVRALSPAATL
                     ERGYAVVQRADGTVLRDPAEVSPGDDLRVRVACGELHATVTGR"
BASE COUNT          385 a         1121 c         1148 g          385 t
ORIGIN      
        1 ccgtgccgcc gaggggcacc tccgcggcgc gcggcagcgc ggtctgcagg cgctccaccc
       61 cgtagcggat cgccgcggcg tagccaccga actcctgccc gagcgtgacg ggcgtcgcgt
      121 ccatgaggtg cgtgcgcccc gacttcacga cgtccgcgaa ctcggtcgac ttggccgcga
      181 gggtgcccgc gaggtgctcg agcgccggga tgaggtcgcg cacgacgccg gccgtggccg
      241 cgacgtgcac cgaggtcggg aacacgtcgt tcgacgactg ggacgcgttg acgtggtcgt
      301 tggggtggac ctcgcgaccc aggcgcgcgg ccgcgagcgt cgcgatgacc tcgttcgcgt
      361 tcatgttcga cgacgtgccg gagcccgtct ggaacacatc gatcgggaag tcccggtcgt
      421 gcaggcccgc agcgacctcg tcggcggctg cgacgatcgc ggccgcgagg ttggcgtcca
      481 ggaccccgag ctcggcgttc gcgagggctg cggccttctt gatgcgggcg agagcctcga
      541 tgtggctgcg ctcgagacgc gtgcccgaga tggggaagtt ctcgaccgcc cgctgcgtct
      601 gtgcgcggta gagcgcgtcc gcggggacgc gcacctcacc catcgtgtcg tgctcgatgc
      661 ggaaaccggg acccgtcgtt gcgtcgctgc tcatgggcgg cattcttgca tcgtcgcggg
      721 cggcccctgc gatccgtcca gcggtccgag cggtcggccc ggtgctcccg ccgatgcccc
      781 gctgctgact caggcgccga tgtcgccgag cgtctcggcg atgtgcacgc gaccgtccgc
      841 gagcgtgtac tcgaggccga cgatcgcaca ccggccctcg tcgaccgcgg ccgcgagcgc
      901 ggccgagtag ccctgcagca tgctgacggt gtgccgcacg tgctcgtgcc ccagctcggc
      961 tgcggagagc gaggcgagcg cacgccggtc ctcgccaccg gcgatgttga cgatgctcgg
     1021 gatgacgcgg tcgacgatcg cgcggacgaa cccccgcggc agctcgcccg tggtcaacgc
     1081 cgtggtcgct gccgcgacgg cgccgcagct gtcgtggccg agcaccacga cgagcgacgc
     1141 gtcgagcagc tcgacgccgt actcgatcga gccgatgacg gtcgtgtcga gcacgtgacc
     1201 cgccgtgcgg acgacgaaga cgtcaccgag cccctggtcg aagatgatct cggccgcgac
     1261 ccggctgtcg gagcacccga agatcacggc gaacgggtgc tgctcgcggc tcagcgacgc
     1321 ccggtggtcg atgccctgcg acgggtgctc catcgccccg cggacgaagc ggtcgttgcc
     1381 gtcgcgcagc gcggcccagg cctcggcggg cgtcatggtc accgtcaggc ctcgccgagc
     1441 tcgaccgacg aaggcgggtt cgcaccggcc cgcgacacgg tgatcgcggc gatgcgcgcg
     1501 caccggtcga gcacacgctg gagcgtcgcg gcgtccacgt cggcgagcgc cccgcggcgg
     1561 tccgcgccga gcagcccggc ggaccacagc ccgtcgatga ggccggacat gaacgagtcg
     1621 cccgctccga cggtgtcggc gacctcgacg cgcggggccg ggacggtcag cttcacgccg
     1681 gtggcggtgc acgcgaaggc gccgtcgccg ccgtgcgtca cgacgacgag gccgggaccg
     1741 agacgcgccc actcctcggc gatgtcggtg ggcgcgacac cggggtgcag ccactcgagg
     1801 tcctcgtcgc tgaccttgac gacgtccgac agcgcgacca gcgcctcgac acggggacgc
     1861 gtggccccgg gtgtccccat gagcgcgggc cgcaggttcg ggtcgtaggt gatggtcgac
     1921 gacgcacggt gcgccgcgac gagctgggcg acggccgcgc cgccgggcgc gagcaccgcg
     1981 gcgatcgagc cggtgtgcac gacgagcggg tcgacgacgg gcgcgtccca ccgtgccggc
     2041 aggtcccagg tcaggtcgaa gtcgtaggtc gcgacgccgt ccgcgtcgag ggtcgcggtc
     2101 gcgaccgagg tcgacgccgg ctgcgtgtcg cccgtcagga cccgcaccgc ggaggcctcg
     2161 aggtgtcggc gcaccgcgtc gccgcgggcg tcctgcccga gccacgtcag caggtcgacc
     2221 ccgcgtccga ggcggccgag cccgatcgcg acgttcatcg ggctgccgcc ggggtgctcc
     2281 gcgacagagc cgtcggtgcg gcgcacgacg tcgatgagcg cctcgccgac gacgagcgcg
     2341 cggggcacga gcgcgccggg tgcggcgtcg tcgcgcgtca tcgcacaccg cccgactgct
     2401 gtgcggcgtc ggccgcgtcc tcggtcgagg tgggcgagcc gtcgccgcgc gacgactcca
     2461 gccgccggcg cgcaccgtcg agccactcct ggcagcgggc cgcgagcgcc tcgccgcgct
     2521 cccacagctc gagcgactcc tcgaggctcg cgccgcccgc ctcgagccgg gccacgacgg
     2581 ccacgagctc gtcgcgggcc tgctcgtagc tcaggcccgt gaggtcgatc gcatcgggtc
     2641 gcgggctctc gggctcgccg tggactggct gggtgggtgc gggctgggat gctcccgctg
     2701 aaggcttcgt cggcacgcgc acagtcaacc agtcgccgcc cacggcgggc gacacaaggc
     2761 gccgccgagg tcgctaccgg ccggtgacag tcgcgtgcag ctcgccgcac gcgacccgca
     2821 cgcgcaggtc gtcacccggg ctcacctcgg cgggatcgcg cagcaccgtg ccgtcggccc
     2881 gctggacgac ggcgtacccg cgctcgagcg tcgccgcggg cgagagcgca cggacttgcg
     2941 cggcgagccg gccgcactgc cccgcggcaa gcgtcagggc ccggtcgagc gcgctcccgg
     3001 cggcggcccg caggcgacgg acgtcccgct cgggcccgt
//
LOCUS       DASRKH010000384         2791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_29388, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000384 DASRKH010000000
VERSION     DASRKH010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29388"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="VFC06_06155"
     CDS             <1..864
                     /locus_tag="VFC06_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZL81501.1"
                     /translation="QTVIRDLSFDVHAGETFGFLGSNGSGKTTTLRALLGIYQPTAGV
                     LHIGGKVFEPRDGARLGYLPEERGLYKKEPVLDVMVYFGRLKPLSRDAAAAWSRTYLE
                     RVSLADKATTRLDKLSGGQQQKIQLGVTIMNDPELLILDEPTQGFDPVNRRLLMDIID
                     EQKRAGATVIMVTHQMDEVERLCDRVVLLKDGTAEAYGTVEDVQDKYGGTVVRLLYAG
                     PLPTSDLFDVISADGSSAELAPRAGTDETEILRELLGHGVAVRSFTPARTSLEEIFLR
                     VYGNQNELVGV"
     gene            868..2094
                     /locus_tag="VFC06_06160"
     CDS             868..2094
                     /locus_tag="VFC06_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZL81502.1"
                     /translation="MAQHNLGTVVGFEFLRVVTKKRFWFAALAVPIALAAVFALVFIS
                     NSSTDQTAKAQKNAQFTFTYTDESGLVTTAVAATFGGTSAADGDQAVADVKNGTVDAY
                     FAYPADPATQPVRVYGVDRGIFENGKYDAVARQILVAGAQQTIGSPALSAMAQGDVKI
                     TSETYKDGQVSGGLDTVIPPLLFLLIFYISIVMLGNQMLNSTLEEKENRVTEMILTTM
                     NPTTLIVGKVISLFMVGAVQMITFALPVVIGYIFFRSNLNLPDVDLSHLVLEPQAMVV
                     GFLLLLGGFLLFTGTLVAIGAMVPTVKEAGTYFAPLMIMIFVPFYVVSVIVSDPHAVI
                     VQVFTYFPYTASVTAMLRNGLGSLTLAESGIVIVELFGLGFVALRLAVYLFRYGSIEY
                     SKKLALRSVLRRKSRA"
     gene            2345..>2791
                     /locus_tag="VFC06_06165"
     CDS             2345..>2791
                     /locus_tag="VFC06_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013601188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZL81503.1"
                     /translation="MAAESAHPTGPGYEHVLVARDLVRRYGDLTAVDGVSFHIDPGET
                     YGLLGPNGAGKTTTISIVAGLIPAHAGTVTVAGRPMGPGEVVGKRSIGLVPQDLAIYP
                     ELTARENLAFFGRLQGMRGKPLARRIDEVLDLIALSDRAKDATKEFS"
BASE COUNT          469 a          958 c          899 g          465 t
ORIGIN      
        1 cagacggtga tccgggacct gtcgttcgac gttcacgcgg gggagacgtt cgggttcctg
       61 ggcagcaacg gatcgggcaa gacaaccacg ctccgagcgc tgctcgggat ctatcaaccc
      121 accgcgggtg tcctgcacat cggggggaag gtcttcgaac cgcgcgacgg tgcccggctc
      181 ggatacctgc cggaggaacg cgggctctac aagaaggagc ccgtcctgga cgtcatggtg
      241 tacttcggcc ggctcaagcc cttgagccgg gacgccgcag cggcctggtc ccggacctac
      301 ctcgagcggg tctccctggc ggacaaggcc accacccggc tggacaagct ctccggcggc
      361 cagcagcaga agatccagct cggcgtgacc atcatgaacg accccgaact gctgatcctg
      421 gacgagccga cgcagggctt cgacccggtc aaccgacgac tcctgatgga catcatcgac
      481 gagcagaagc gcgcgggcgc caccgtcatc atggtgaccc accagatgga cgaggtggaa
      541 cggctctgcg accgcgtcgt cctcctcaag gacggcaccg ccgaggcgta cgggacggtc
      601 gaggacgttc aggacaagta cggcggcacc gtcgtccgcc tgctctatgc cgggccgctc
      661 cccacctcgg acctgttcga cgtgatctcg gcggacggca gctccgccga gctggcgccg
      721 cgcgcgggta ccgacgagac cgagatcctg cgcgagctcc tcgggcacgg cgtagcggtg
      781 cgcagcttca cgccggcacg aacctcgctc gaggagatct tcctcagggt ctatggcaac
      841 cagaacgaac tcgtgggggt ctgagacgtg gcacagcaca atctcggcac cgtcgtcggg
      901 ttcgagttcc tccgggtcgt gacgaagaag cggttctggt tcgccgccct ggctgtcccg
      961 atcgccctcg ccgcggtgtt cgcgctcgtg ttcatcagca actcgagcac cgaccagacc
     1021 gcgaaggccc agaagaacgc gcagttcacg ttcacctaca ccgacgagtc cggcctggtg
     1081 accaccgcgg tcgccgcgac cttcgggggg acctcggcgg cggatggtga ccaggccgtc
     1141 gccgacgtga agaacggcac ggtcgacgcg tacttcgcct accccgccga tcccgcgacc
     1201 cagcccgtcc gggtctacgg ggtcgaccgg gggatcttcg agaacggcaa gtacgacgcc
     1261 gtcgcgcgac agatcctcgt cgcgggcgcg cagcagacga tcggctcacc cgccctcagc
     1321 gcgatggctc agggcgacgt gaagatcacc tcggagacgt acaaggacgg gcaggtgtcc
     1381 ggtggtctgg acaccgtcat cccacccctg ctgttcctgc tgatcttcta catcagcatc
     1441 gtcatgctgg ggaaccagat gctgaacagc acgctggagg agaaggagaa cagggtcacg
     1501 gagatgatcc tcaccaccat gaaccccacg acgctgatcg tcgggaaggt catctcgttg
     1561 ttcatggtcg gcgcagtcca gatgatcacc ttcgcgctgc ccgtggtgat cggctacatc
     1621 ttcttccggt cgaacctcaa tctgcccgac gtcgacctgt cccacctggt cctcgagccc
     1681 caggcgatgg tcgtcggctt tctgctgctc ctgggtgggt tcctcttgtt caccggcacc
     1741 ctggtcgcca tcggcgccat ggtcccgacc gtcaaggagg cgggcaccta cttcgctccg
     1801 ctgatgatca tgatcttcgt cccgttctac gtcgtgagcg tgatcgtcag cgatccgcac
     1861 gcggtgatcg tccaggtctt cacctacttc ccgtacaccg cgtccgtcac cgcgatgctc
     1921 cgcaacggcc tcggctccct cacgctggcg gagtcgggga tcgtcatcgt cgagctgttc
     1981 gggctcggtt tcgtcgcgct acggctggcg gtctacctgt tccgctacgg ctcgatcgag
     2041 tactccaaga agctcgcgct gcgcagcgtc ctgcgccgca agagccgcgc ctgactcacg
     2101 gcaggtactg ctcctcgatc gcggtgacgg tgccgccgct gatcgtcagc cggtagaggc
     2161 tcaagaggaa tcggtcggcg tcgggtccgg tctgctccgc tcagtgccag gaggggattc
     2221 ggaggcgaag atgcgtgctc gtgaacgtgc gcgaaaaccc ccaagaactc gtcacgatcg
     2281 gtgccagact gaccgggcac agcggcatca gtcgacgaca ggcgggcagg cgttgagcac
     2341 ccaggtggca gccgagagcg cacaccccac cggtcccggg tacgagcacg tgctcgtcgc
     2401 gcgcgacctg gtccgtcgct acggggacct gaccgccgtc gacggcgtgt ccttccacat
     2461 cgaccccggc gagacctacg ggctgctggg tcctaacggt gcgggcaaga ccacgacgat
     2521 ctccatcgtc gccggtctca tccctgccca tgccggcacg gtcacggtgg ccgggcggcc
     2581 gatgggaccg ggtgaggtcg ttggcaagcg gtccatcggc ctggtgcccc aggacctcgc
     2641 catctacccc gagctcacgg cccgggagaa cctcgccttc ttcggccggt tgcagggcat
     2701 gcggggcaag ccgctggcgc ggcgtatcga cgaggtcctc gacctcatcg ccctgtccga
     2761 ccgcgcgaag gacgccacca aggagttctc c
//
LOCUS       DASRKH010000385         3944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_33597, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000385 DASRKH010000000
VERSION     DASRKH010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33597"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..1410)
                     /locus_tag="VFC06_06170"
     CDS             complement(43..1410)
                     /locus_tag="VFC06_06170"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZL81504.1"
                     /translation="MTSAFARRRAALSPSGFYGWHVVAYSSIALAATGPGQTVGVSLF
                     IDPLIRELGLSRSSVATAYLIGTLGGAIALPSIGRALDRFGVRRTMAVIGAAFGAVLI
                     ALSFVTSVAGLTIGFVGLRMAGQGALGLTASTAVALWFHRRRGMAAGLVSAFGAVGIS
                     SIPLLIEPLLADLGWRNVWRIEGLVIWAVVIPLGLLAMRDRPADLGQHPDGPVVVTQE
                     QVTPHRAEGVSRAAALRYPYFWLVSTAVALTGLLTTAVAFHQISLLTERGLSPAQAAA
                     NFVPQTVAGLVATLAAGYLMDRFAARWMIIASMAVLSAGLWWGMGVSPGVSAFAFGAM
                     LGAAGSMIRAVETVALPRYFGTLHIGSIRGLVASISVAGTAAGPVLFAAVFERTGSYS
                     PVLLASAFAPLVIIVWALIAKEPVLGRSDRRPQSTPNPDDRDPAPTEAPMPGAAVPTG
                     SPHVG"
     gene            complement(1446..3038)
                     /locus_tag="VFC06_06175"
     CDS             complement(1446..3038)
                     /locus_tag="VFC06_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HZL81505.1"
                     /translation="MTAPTQATTAVEKQSARRRSIAVISHPDAGKSTLTEALALHARV
                     ISETGAVHGKHGRAGVVSDWLEMEQKRGISITSAALQFSFEDVVINLLDTPGHADFSE
                     DTFRVLTAVDAAVMLLDAAKGLEPQTLKLLEVCRLRGVPVITFVNKWDRPGREALALC
                     DELEQRVNLRATPVTWPIGEAGDLRGLLDARTKDVTLYQRTAGGAALAEEQHLSLDAA
                     RTHVGAELDEALDSLELLEPLDLDSFHAGTTTPLLFGAALSNIGVRQLLDVIAEFAPP
                     PSARVGADGTERAIGAPFSGFVFKMQAGMDTKHRDHVAFVRVCSGEFQRGMTVTHATS
                     GRPFATKYALSLFGRAREVIETAYPGDIVGLVNASNLAVGDTLFAASGPAITFPPMPA
                     FAPEHFVTARVADPGRSKQFRRGIAQLQQEGVVQVFRSESRGDASPILAAVGPLQFEV
                     ATFRMEHEFHASIRMDHLDYALARPATDADARLLAGRHGVEMVGRSDGALHALVRDIW
                     RLKSLHRETPDLTLEPLPVGAR"
     gene            complement(3035..3451)
                     /locus_tag="VFC06_06180"
     CDS             complement(3035..3451)
                     /locus_tag="VFC06_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012805861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZL81506.1"
                     /translation="MGFNHKQIGEVAERTGLSLRTIRYYEEVGLVTPSSRSQGGFRQY
                     TEVDVARLELVKRMKPLDFSLEEMRELLTALDALESDATHVDDREALLERLTKFRLTA
                     EERCVSLRAQLESAESFASSLRREIRQQSRVAAQTQ"
     gene            complement(3843..3944)
                     /locus_tag="VFC06_06185"
     CDS             complement(3843..3944)
                     /locus_tag="VFC06_06185"
                     /inference="COORDINATES: protein motif:HMM:NF019643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HZL81507.1"
                     /translation="MPDNYGTNYERLREVKRMYDPQNLFHLNQNIVP"
BASE COUNT          673 a         1272 c         1380 g          619 t
ORIGIN      
        1 cgctgagcga ctgcgtcgag cggttcgaga catggaccga cactacccga catggggtga
       61 gcctgtgggg accgctgcgc caggcatggg cgcctcggtc ggggccgggt cacggtcgtc
      121 cgggttcggc gtgctttggg gtcgacggtc gctccggccg aggacgggct ccttggcgat
      181 gagggcccac acgatgatga ccagcggcgc gaaggcggac gcgaggagaa cgggcgagta
      241 ggagccggtg cgctcgaaca cggccgcgaa cagcaccggc ccggcggcag ttcctgcgac
      301 gctgatcgag gcgacgaggc cccggatcga gccgatgtgc aacgtgccga agtaccgagg
      361 caaggcgacc gtctcaaccg ctcggatcat gctgccggcg gccccgagca tcgcgccgaa
      421 tgcgaacgct gacacccccg gagagacgcc catcccccac cacagcccgg cggacagcac
      481 tgccatcgag gcgatgatca tccagcgggc ggcgaagcgg tccatcaggt accccgcggc
      541 aagcgtggcc accaggcctg ccacggtctg cgggacgaag ttcgccgcag cctgggcggg
      601 gctcagtccg cgttcggtga gcaggctgat ctggtggaac gccacagcag tggtcagcag
      661 tccggtgagg gcgaccgcgg tggagaccaa ccagaagtac gggtaccgaa gtgccgcagc
      721 tcgactgaca ccctcggccc ggtgcggtgt cacctgttcc tgggtgacca cgaccggacc
      781 gtctgggtgc tgtccaagat cggcggggcg gtcgcgcatc gcgagcagtc caagggggat
      841 gacaacggcc cagatgacga gcccctcgat tcgccagacg tttcgccagc cgagatcggc
      901 caggaggggt tcgatgagca aggggatcga ggagatcccg acagcaccga acgcgctgac
      961 caaccctgcg gccatgccac ggcgccggtg gaaccacagc gcgacggccg tgctggccgt
     1021 caggccgagc gcgccctgtc ccgccatccg caagccgacg aagccgatgg tcagtccggc
     1081 gacggacgtg acgaaagaca atgcgatcag cacggcgcca aacgctgccc cgatcacggc
     1141 catggtgcgt cgcacgccga accggtccag ggcccgtccg atcgatggca gggcgatcgc
     1201 cccgccgagg gtcccgatca ggtaggccgt ggccacgctc gacctgctca ggccgagctc
     1261 gcggatgagc gggtcgatga acagcgagac cccgacggtc tgacccggtc cggtggcggc
     1321 cagggcgatg ctggagtagg ccacaacgtg ccagccgtag aagccggatg gggacaacgc
     1381 ggcgcgtcgg cgtgcgaatg ctgaggtcat gtggctcctg tgggcttccg gtgaggtggt
     1441 gcgggctagc gcgcgccgac tggcaacggc tcgagcgtga ggtctggggt ctcgcggtgc
     1501 agcgacttta gccgccagat gtcccgaacg agagcgtgca gagcgccgtc cgacctcccg
     1561 accatctcga ccccgtggcg gccagcgagc aggcgtgcat cggcgtcagt ggccggccgg
     1621 gccaaggcgt agtccaggtg gtccatgcgg atcgaggcgt ggaactcgtg ctccatgcgg
     1681 aaggtggcga cttcgaactg cagcggtccc actgcggcca gaatgggtga ggcgtcgccg
     1741 cgggattcgg atctgaagac ctgcacgacg ccctcttgct gcagttgagc gatccctctc
     1801 cggaactgct tggagcggcc gggatcggcg acccgcgcgg tgacgaagtg ctccggggcg
     1861 aaggccggca tcggcgggaa cgtgatcgcg gggccggatg cagcgaagag cgtgtcgccg
     1921 accgccaggt tgctcgcgtt gaccaagccg acaatgtcgc cagggtaggc ggtttcgatg
     1981 acctctcgtg cccggccgaa gagcgaaagc gcgtacttgg tcgcgaaggg tcgaccggag
     2041 gtcgcgtggg tcacggtcat accgcgttgg aactcccccg agcagacccg gacgaatgcg
     2101 acgtggtcgc gatgtttcgt gtccattccg gcctgcatct tgaacacgaa gccgctgaag
     2161 ggtgcgccga ttgcgcgctc cgttccatcc gcgccaacgc gggccgacgg tggcggcgcg
     2221 aactcggcga tgacgtcgag gagctgtcgc acaccgatgt tggacagcgc cgcaccgaag
     2281 agcaggggcg tcgtggtacc ggcgtggaag gaatcgagat cgagtggctc aagcagctcg
     2341 agggagtcga gtgcttcgtc gagctcggcg cctacgtgcg tccgggccgc atccaagctg
     2401 aggtgctgct cctcggccag cgccgcgcca cccgctgtcc gctggtacag cgtgacgtcc
     2461 ttggtacggg cgtccaggag cccccgcagg tcgcctgcct ccccgatcgg ccaggtcacg
     2521 ggggtggcac ggagattgac ccgctgttca agctcgtcgc atagcgccag ggcctcgcgg
     2581 ccgggccggt cccacttgtt gacaaaggtg atgaccggga caccgcggag tcgacacacc
     2641 tcgagcagct tcagagtctg cggctccagg cccttggcag cgtccaggag catcactgcg
     2701 gcatcgaccg cggtcaacac gcgaaacgtg tcctcggaga agtccgcgtg gccgggcgtg
     2761 tccagaaggt tgatgacgac gtcctcgaag gagaactgga gcgcggcgga ggtgatcgag
     2821 atgccacgct tctgctccat ctcgagccaa tccgagacca ctccggcgcg tccgtgcttg
     2881 ccgtggacgg ccccagtttc actgatcacg cgggcgtgca aagccaacgc ctcggtcagt
     2941 gtggacttac cggcatccgg gtgggagatt acggcgatgc tgcgccgacg agcgctctgc
     3001 ttctcaaccg cggtcgtggc ctgcgtcggc gcggtcactg cgtttgggcg gccacccgcg
     3061 actgctgccg aatctcccgg cggaggctgc tggcgaaact ctcggcggac tcgagctggg
     3121 cccgcaggct cacgcatcgc tcctcggcag taagtcgaaa cttggtgagt cgctcgagta
     3181 gagcctcgcg gtcgtcgaca tgcgtagcgt ctgactcgag ggcatcgagc gcggtcagca
     3241 actcgcgcat ctcctcaagg ctgaagtcga gcggtttcat ccgcttgacc aattccagcc
     3301 gcgccacatc cacctcggtg tactgcctga agcccccttg agaccggctt gacggcgtga
     3361 cgagtccgac ttcctcgtag tagcgaatgg tgcgcagaga cagtccggtg cgctcggcca
     3421 cctcgccgat ctgcttgtgg ttgaaaccca tgatgccttc cgctcgatcg cagatcagtc
     3481 taccgtcacg ggagggatgg cacggcacgg gccaattcgg accacatgat gcggcatcca
     3541 caacacttcc gtcacgtcag attcgcgacg tgcctcgctt ccatgacgtc agattcgcga
     3601 cgtgtctcgc ccgggttgag cgaggaccgg acggcattgc gccgacgcga ccgtcaccag
     3661 ggagagccgg tgtcttgtcg gtgcctctcg agtcgacgtt tcggcggttc atcaccgtat
     3721 ctgctcgccg gatcacatca ggcccgtcct cgcaggcgac gacacaccgc gctgtggccg
     3781 ggcgatacgg gcacacgcac cgaatgcccc gcgtcggtgg cggggcattc ggcaggagct
     3841 tcctacggga cgatgttctg gttgaggtgg aacaggttct gcgggtcgta catgcgcttc
     3901 acctcgcgca gccgctcgta gttcgtgccg tagttgtccg gcac
//
LOCUS       DASRKH010000386         2754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_19084, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000386 DASRKH010000000
VERSION     DASRKH010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19084"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="VFC06_06190"
     CDS             complement(<1..950)
                     /locus_tag="VFC06_06190"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009773638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HZL81508.1"
                     /translation="MILPAHHPLVAGTASASPLVRALRADRPERTPVWFMRQAGRSLP
                     EYREVRKGTTMLEACLDPGLVAEITAQPVRRHGVDAGILFSDIVVPLLVAGVDVTIEP
                     GVGPVFAKPISTAADVAALPSLGATGDRGGFDRALDVVRVSVGASLEQLGNTPLIGFA
                     GAPFTLAAYMVEGRPSRDHLAARRLMIADPEAWDALMVWAADAAGLFLRAQVLAGASA
                     VQLFDSWAGSLPRERYAARCAPASARAFGHVADLDVPRIHFGTHTGHLLEDMHGAGAT
                     VMGIDVTVPLDEAAARLPGVPLQGNIDPAVLGAPWEAIEA"
     gene            993..2285
                     /locus_tag="VFC06_06195"
     CDS             993..2285
                     /locus_tag="VFC06_06195"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:NF000750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HZL81509.1"
                     /translation="MALLSLVASHHNKDLNTLEQLSVGSDRLGNAVIGSTVTGAVVLP
                     TCNRFELYLDVVDPVAARAEVVAALSSMTSLDVDVIDASLLAYEGDDAVEHLMSVTSG
                     LESMVVGEREISGQVRRAHIDAREAGHLSPSLDRVFQSALRTSRVVASSTGLGTSGRS
                     VMSVAFDLADATVAWRGASALVVGTGALAETGIGVLRGRGARVTGVYSPSGRGGEFAG
                     LHDLDVVPAEGLVDALEEADVVLACSGGESIVLTPSLVAQAMNSRRHSLTLIDLALHR
                     DVAPGVELLRGVHVVRLEDVKSHAPEESMAALETARAIVAHAVARFSTSESGRSVDPA
                     IVALREHVFGLLEREVARVRPGPGVDPLGYTQAAAIEAALRHFAKALLHTPTVRAKEM
                     AESGQTDEYLNALSTLYGLDVAVPDDSCPAQEIDSSSA"
     gene            complement(2305..>2754)
                     /locus_tag="VFC06_06200"
     CDS             complement(2305..>2754)
                     /locus_tag="VFC06_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014921106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HZL81510.1"
                     /translation="VGGQELGYLPGSEAEKMNPWAQAVFDTLGAVVSSAVVEEVMARG
                     MLEVLPLTHIRGRSLHDAFVIVDEAQSLERNVLLTMLSRIGRDSRVVLTHDVAQRDNL
                     RVGRHDGVAAVIETLKGHPLFAHVTLERSERSEVAALVTDLLERLDL"
BASE COUNT          418 a          968 c          938 g          430 t
ORIGIN      
        1 tgggcctcga tcgcctccca cggcgcgccc agcaccgccg ggtcgatgtt gccttgcagg
       61 ggcacgccgg gaagcctggc ggccgcctcg tcaagcggga ccgtcacgtc gatccccatg
      121 accgtcgccc cagcaccgtg catgtcctcc agcaggtgcc ccgtgtgggt cccaaaatga
      181 atccgcggca cgtcaaggtc cgcgacgtgg ccgaacgcgc gggccgaggc cggggcgcag
      241 cgcgcggcgt agcgctcgcg gggcagcgaa cccgcccacg aatcgaacag ctgcacagcg
      301 ctggccccgg cgagcacctg cgctcgcagg aacagcccgg cagcgtcggc cgcccacacc
      361 atgagcgcat cccacgcctc ggggtcggcg atcatgaggc gcctggcggc gaggtgatcg
      421 cgcgagggtc gcccttcgac catgtatgcg gccagcgtga acggggcgcc ggcgaatccg
      481 atgagcggcg tgtttcccag ctgctcgagc gaggcgccca cggagacccg caccacgtcg
      541 agcgcgcggt caaagcctcc ccggtccccg gtggctccga gcgagggaag cgcggcaaca
      601 tcggcggcag tagaaatcgg cttggcaaac acgggtccca cgcccggctc gatcgtcacg
      661 tccaccccgg ccacgagcag cggcaccacg atgtcggaga agaggatgcc cgcgtcgacg
      721 ccatgtcgcc tcaccggttg cgccgtgatt tcggccacga gcccggggtc gaggcacgcc
      781 tcgagcatcg tggtgccctt gcgcacctcc cgatactccg ggagcgagcg ccccgcctga
      841 cgcatgaacc agacgggggt gcgctccggg cgatcagccc gcagagcgcg caccaagggt
      901 gaggccgatg ctgtgccggc tacgagcggg tgatgtgcgg ggagaatcat gccttgattc
      961 tcccccactc ctggccgtgt cagtattggg gcatggcatt gctgtccctc gtggcgagcc
     1021 accacaacaa agacttgaac accctggagc agttgagcgt tggctctgac cgcctgggga
     1081 acgccgtcat tggctccaca gtgaccggcg ccgtcgtgtt gcccacgtgc aatcgcttcg
     1141 agttgtacct cgacgtggtt gaccccgttg ccgctcgagc cgaggtggtc gcggcgctgt
     1201 cgtccatgac gtctctcgat gtggacgtca ttgacgcctc gctcttggcc tacgagggtg
     1261 acgatgcggt cgaacacctc atgtcggtga cttccggact cgagtcgatg gtggtgggcg
     1321 aacgcgaaat ctcgggccag gtgcgccgcg cccacatcga cgctcgcgag gccggccact
     1381 tgtcaccctc gctggatcgc gtcttccagt ctgcgctgcg cacatcgcgc gtcgtcgcat
     1441 cgtccaccgg cttggggacc tccggacggt ccgtcatgtc cgtcgccttc gaccttgccg
     1501 atgccaccgt cgcctggcgc ggggccagcg cgcttgtcgt gggcaccggt gcccttgccg
     1561 agacggggat cggcgtgctg cgcggtcgcg gcgcgcgcgt gaccggcgtg tattcgcctt
     1621 cagggcgcgg gggcgagttt gcgggactcc acgaccttga cgtggtgccg gccgagggcc
     1681 tcgtggatgc gctcgaagag gcggacgtcg tgctcgcgtg ctccggcggc gagtcgatcg
     1741 tgctgacccc ctcactcgtc gctcaggcga tgaactcgcg taggcactcg ctgacgctga
     1801 tcgacttggc gcttcatcgc gacgtcgccc ccggcgtgga gttgctgcgc ggcgtccacg
     1861 tggtgcggct cgaggacgtc aagtcccacg ctcccgagga atccatggcg gcgctcgaga
     1921 ctgcgcgcgc catcgtggcc cacgccgtcg cccgcttctc gaccagcgag tcgggtcgat
     1981 cggtcgaccc cgccatcgtc gccttgcgcg agcacgtatt cggccttctt gagcgggagg
     2041 tggcacgagt gcggcccggc ccaggcgtcg acccgttggg ctacacccag gctgccgcga
     2101 tcgaggcagc gctccggcat ttcgcgaagg cgctgctcca cacgcccacg gtgcgcgcca
     2161 aggagatggc ggagtccggt cagaccgacg aatacctgaa cgcgctcagc acgctgtacg
     2221 ggctcgacgt cgcggtgccg gacgactcct gcccggcgca agaaatcgac tcgtcaagcg
     2281 cgtagcaccc cagcggggca gcccctagag gtccagccgc tccagcaggt ccgtgaccag
     2341 cgcggcgact tcggaacgct cggaacgctc cagcgtcacg tgtgcgaaca gcgggtgccc
     2401 tttgagcgtc tcgatcacgg cggcgacacc gtcgtgacga ccgacccgca ggttgtcgcg
     2461 ctgggccacg tcgtgggtga gtaccacgcg cgagtcacgg ccgatgcggc tcagcatcgt
     2521 cagcagcacg ttgcgttcca gggactgcgc ctcgtccacg atcacgaacg cgtcgtgcag
     2581 agaacgaccg cgaatgtggg tgagcggcag cacctcgagc atgccgcgcg ccatgacctc
     2641 ctcgaccacc gcggaactca ccacggcgcc cagggtgtcg aacacggctt gggcccaggg
     2701 gttcatcttc tcggcctcgg agccgggcag gtagcccagc tcctggccgc ccac
//
LOCUS       DASRKH010000387         3397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_43024, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000387 DASRKH010000000
VERSION     DASRKH010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43024"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            449..760
                     /locus_tag="VFC06_06205"
     CDS             449..760
                     /locus_tag="VFC06_06205"
                     /inference="COORDINATES: protein motif:HMM:NF013938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81511.1"
                     /translation="MMSLPPARDASDDVLRALDPDLTGSALLPYAVHRNAEVRAAVAA
                     RADCPAGALISLGHDHDFVVLEALLRNVRTPSSVVRALADHRNQRISDLAVQRLRNGY
                     R"
     gene            898..1119
                     /gene="infA"
                     /locus_tag="VFC06_06210"
     CDS             898..1119
                     /gene="infA"
                     /locus_tag="VFC06_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HZL81512.1"
                     /translation="MAKKDGVIEVEGSVVEALPNASFRVELTNGHLVLAHISGKMRQH
                     YIRILPEDRVVVELSPYDLSRGRIVYRYK"
     gene            1165..1278
                     /gene="rpmJ"
                     /locus_tag="VFC06_06215"
     CDS             1165..1278
                     /gene="rpmJ"
                     /locus_tag="VFC06_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HZL81513.1"
                     /translation="MKVKPSVKPICEKCKVIRRNGRVMVICENLRHKQRQG"
     gene            1405..1773
                     /gene="rpsM"
                     /locus_tag="VFC06_06220"
     CDS             1405..1773
                     /gene="rpsM"
                     /locus_tag="VFC06_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019174662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HZL81514.1"
                     /translation="MARLVGVDLPRDKRMEIALTYIFGIGRTRSHQILAATGVDADLR
                     VKDATDEQLVALRDFIEANFVIEGDLRREVQGDIRRKVEIGNYQGLRHRRGMPVRGQR
                     TKTNARTRKGPKKTVAGRKK"
     gene            1777..2166
                     /gene="rpsK"
                     /locus_tag="VFC06_06225"
     CDS             1777..2166
                     /gene="rpsK"
                     /locus_tag="VFC06_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HZL81515.1"
                     /translation="MAAPVRKPRKKLKKTVAHGQAHIKSTFNNTIVSITDPSGAVVSW
                     SSSGQVGFKGSRKSTPYAAQMAAEAAARKAQDAGMSKVDVFVKGPGSGRDTAIRSLTA
                     AGLEVTSIKDVTPQAHNGCRPPKRRRG"
     gene            2328..3317
                     /locus_tag="VFC06_06230"
     CDS             2328..3317
                     /locus_tag="VFC06_06230"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012877435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HZL81516.1"
                     /translation="MLIAQRPTLTEKVLTDNRSQFSLKPLEPGFGYTLGNSLRRTLLS
                     SIPGAAVTSVRFEGVLHEFTTIEGVKEDVTEILLNLKNLVVSSEHDEPVIMYLRKSGA
                     GAVTAADIAPPAGVEIHNPDLHVATLNSKAKFEVELTVERGRGYVPAALNKSYDAEIG
                     QIPVDSIYSPVLKVTYKVEATRVAQRTDFDELVLDVETKSSISPRDAVASAGSTLVEL
                     FSLARSLNEAAEGIEIGPSDTDEALEEDYNMPIEDLTLTQRSYNCLKREGIHTVGELT
                     ARSETDLMDIRNFGQKSITEVKEKLAELGFTLKDGGQEYDPTTSGVFFSGGDE"
BASE COUNT          662 a         1098 c         1035 g          602 t
ORIGIN      
        1 cgggggccac gccgcgcacc gagcctccgg cgcgacgcgc tgccacggag accgcgtccg
       61 cccgcgtagc acccgttcgc ggcttccgct tctctgaggt tgagtagtac ccctcagcag
      121 acaagaagcg agtgttccga cgggggaccc actgtctcgc ggcgcaccgc aatcctgcag
      181 tgagggccgt cgtggcctca cgcgggggct gtcgccattt ctttgtggcg cgccggtcgc
      241 gcgcggggag catgccggcc gaactccacg accgggtccc gcctaggtga ggcggtttcg
      301 ccccccgctt gagcaaggcg atgcacactc tcttcgcgac acagaaatgt cgagttcctc
      361 acaaaagtcg attttgccct accatccggc ctacgctgtc agtgatgcac ggggtgacgg
      421 actccccggc gaaagggtgg acaaggcgat gatgtcactt cccccggcac gcgacgcatc
      481 ggacgatgtt cttcgcgccc ttgacccgga cttgactggt tcggcgctcc ttccgtacgc
      541 ggtgcaccgc aacgccgagg tgcgggccgc agtcgcggcg agggccgatt gccctgcagg
      601 agcgctcatc tccttgggtc acgaccacga cttcgtggtt cttgaggcgc tactgcgcaa
      661 cgtgcgcacc ccgtcctcag tggtcagggc actcgcggat caccgcaatc agcggatctc
      721 cgatctggct gtccagcgcc tccgcaacgg ctaccggtag ccgtttgcgg gcgctggccg
      781 cagccgcggc gggtggctgg cgatgcgcga tttgagtctt atccacatct ccggtattct
      841 cgacggtcgg gcgtttttgc gcgcctgcac acccattcat cgacgttagc ggagttcatg
      901 gccaagaaag acggcgtcat tgaggtagaa ggctcagtag tcgaagcgtt gccgaacgcg
      961 tcattccgtg tggaactgac gaacggccac ctcgtgctag cccacatctc agggaagatg
     1021 cgccagcact acatccggat ccttcctgag gaccgcgtag tggtggaact gagcccttat
     1081 gacctgtccc gcgggcgcat cgtctaccgc tacaaataag cgccgccgcc ggtgtgatgc
     1141 cggcgttggt cgaggaaaca aaccatgaag gtgaagccga gcgtcaagcc catctgtgaa
     1201 aagtgcaagg tgattcgccg caatggccgc gtgatggtca tttgcgagaa cttgcgccat
     1261 aagcagcgtc agggctagac aaccgattaa gacctcgttg aggcaccggg agaccggagc
     1321 ccgcggctcg gaggccgcgg cccgcactat atccagcggg aagccccagc gacagacctc
     1381 cgactacata tggagtagag aaatatggca agactcgttg gagtcgatct gccgcgtgac
     1441 aagcgcatgg agatcgccct gacctacatc ttcggcatcg gccgtaccag gtctcaccag
     1501 atcctggccg ccaccggagt tgacgccgac ctacgcgtga aggacgccac cgacgaacag
     1561 ctcgtggcac tgcgcgattt catcgaggcg aacttcgtca tcgagggtga cttgcgccgc
     1621 gaggtgcagg gcgacattcg ccgcaaggtc gagattggca actaccaggg tctgcgccac
     1681 cgtcgtggca tgccggtccg tggccagcgc accaagacca acgcgcgcac ccgcaaggga
     1741 cccaagaaga ccgtcgccgg aaggaagaag taggccatgg ctgcacccgt acgcaagcct
     1801 cgaaagaagc tgaagaagac cgtcgctcac ggtcaagcac acatcaagtc gactttcaac
     1861 aacacgatcg tgtcgatcac ggacccctcg ggcgccgtgg tgtcgtggtc gtcctcgggt
     1921 caggttggct tcaagggttc gcgcaagtcg accccctacg ccgctcagat ggccgccgag
     1981 gcagctgccc gtaaggcgca ggatgctggc atgagcaagg tggacgtgtt cgtcaagggt
     2041 cccggctccg gccgtgacac cgcgatccgt tctctgacgg ccgctgggct cgaagtgacc
     2101 tccatcaagg acgtcactcc tcaggctcac aacggctgcc gcccccccaa gcgtcgtcgc
     2161 ggttagttgc tgacgccgcc gcgccccatg gtgcggcggc caggcacgcg tgccgcctta
     2221 gcaccgcaag gtgcctctac ccaagattta ccgacgttga cacttgccgg aacggacagc
     2281 cggcaggttc gagcatcaaa tagcgggtgc tcggaaagga taggaaagtg ctgatcgcac
     2341 agcgtcccac cttgaccgaa aaggtcttga cggacaaccg ctcccagttt tctctcaagc
     2401 cgctggagcc tggcttcgga tacaccctcg gaaactccct ccgtcgcacg ttgctctcgt
     2461 ccattcctgg cgcggcagtc acgagcgttc gcttcgaagg cgttctgcac gagttcacca
     2521 cgatcgaggg ggtgaaggag gacgtcaccg agatcctcct caacctcaag aacctggtgg
     2581 tctcctcgga gcacgacgag ccggtcatca tgtacttgcg caagtctggt gctggcgccg
     2641 tcactgccgc cgacatcgcc ccgcctgctg gcgtggagat tcacaacccg gacctgcacg
     2701 tcgcgaccct gaactcgaag gcgaagtttg aagtcgagct cacggtcgag cgcggacgcg
     2761 gttacgtgcc cgccgccctc aacaagagct acgacgcaga gatcggccag attccggtcg
     2821 actcgatcta ctcgccagtg ctcaaggtca cgtacaaggt cgaggcaact cgtgtggcgc
     2881 agcgcaccga cttcgacgag ctcgtcctcg atgttgagac caagtcgtcg atttccccgc
     2941 gtgacgcggt ggcatcggca ggttcgactc tcgtcgagct cttctcgctc gcgcgtagcc
     3001 tcaacgaggc tgccgagggc atcgagattg gtccttcgga caccgatgag gcgctcgaag
     3061 aggactacaa catgcctatc gaggacctca cgctgacgca gcgttcgtac aactgcctga
     3121 agcgcgaagg cattcacacc gttggcgagc tcaccgcgcg ctccgagacg gacctcatgg
     3181 acattcgcaa cttcggtcag aagtcgatca ccgaggtgaa ggagaagctc gcagagctcg
     3241 gcttcaccct gaaggacggc ggtcaggaat acgacccgac tacctccggt gtgttcttct
     3301 ccggtggcga cgagtaacaa ggagaacaac tcactatgcc tacacccact aagggtcccc
     3361 gtcttggcgg cggtcctgct cacgagcgtc agatcat
//
LOCUS       DASRKH010000388         3313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_42308, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000388 DASRKH010000000
VERSION     DASRKH010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42308"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VFC06_06235"
     CDS             <1..282
                     /locus_tag="VFC06_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81517.1"
                     /translation="VNMYCRGWVLPVAGAVCVRTVSNSASEPDAPIVATAFALAMVLG
                     LLDYYVETQVAHVERARVLGAQADVFHDLEGPAVQMVLPTDPEWRPDSL"
     gene            complement(750..823)
                     /locus_tag="VFC06_06240"
     tRNA            complement(750..823)
                     /locus_tag="VFC06_06240"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(787..789),aa:Pro,seq:cgg)
     gene            complement(1020..1919)
                     /locus_tag="VFC06_06245"
     CDS             complement(1020..1919)
                     /locus_tag="VFC06_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZL81518.1"
                     /translation="MNRATAAALSVGAAGAACVAWGLAEAHAFTTRRVTVPVLAPGQA
                     SLRVLHVSDLHLTPRQRDKIAWAASLAQEQPDLVVVTGDNLAHADAVPAVAEALAGVL
                     SAPGVFVFGSNDYFAPHLKNPLGYFAGPSRALQEQVRLPTDQLRSVFLDAGWHDLNNA
                     RASLDIGGVAVSTVGMDDPHIGRDRMPPPDGRDHGTLHLGVVHAPYLRALHALKADGA
                     DLILAGHTHGGQVCVPGYGALVTNCDLDRRRAKGLSTWPGEALGPDDAWLHVSAGAGT
                     SPFAPVRFACRPEATVLTLTARE"
     gene            complement(1926..>3313)
                     /locus_tag="VFC06_06250"
     CDS             complement(1926..>3313)
                     /locus_tag="VFC06_06250"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HZL81519.1"
                     /translation="DVTTGNILAMSQNREFDPTSKAPYSTAINYAVDRDYGGSRGFSP
                     GSSFKPLVLSQWLDSGHSLSQVISGVPRTWKGPTWRTSCPEGAGYVSDYKVGNVSASE
                     GVQMSVLRATTLSVNTAYVAMTHQLDLCQVRDMALTLGFHRSDGLDFEVVPSVTLGSQ
                     NASPLTMASAYQTFANRGIHCEPRAILSMTKLDGSEVLGPDGKPIVPPEVKCSQVIRA
                     EVADGVAYALTQVMETGTGKYLGIGRPVAGKTGTSQNNAHMWFAGFTPQVVSAVWTGN
                     AEVDVPLQDFTINGVHKWYWYGADISGPVWQDFMTQALKPYPGVGLPGASDAMINGSQ
                     LRVPNVVGLGLSVQAAEWAINDAGFQYAKSAELVYVPGVPAGQIVRQSPAADWTMRAG
                     GVVTFYMSTDALPGWWTKWPSGWDPLTAPADWWGGAWPPADWTKNKPSKGWDPTPVAP
                     PSPTPAPDPKP"
BASE COUNT          569 a         1087 c         1083 g          574 t
ORIGIN      
        1 gtgaacatgt actgccgtgg ttgggtgctg cccgtcgcag gtgccgtatg cgttcggacg
       61 gtgtccaact ccgcgagtga gcctgatgcg ccgatcgttg ccacggcgtt cgcattggcg
      121 atggtgcttg ggctgcttga ctactacgtc gagactcagg ttgcgcacgt ggagagggct
      181 cgtgtgctgg gggctcaggc ggacgtgttc catgatctcg agggcccggc cgtgcagatg
      241 gtgctgccaa ctgaccccga gtggcgccct gattccctat gagcggtcgc aggtgagcct
      301 ctggcgtggc tgaggggcgc tgtcggtact gctaggccgg atgcccgtct cggggctctg
      361 gacggcattg tggcgctccc tgggggctat gttggccaca ctgcgctcgg gcggctcttg
      421 gcagggggct ttcgcactgc aacccgcacc ttcgccagcg cccgggtcct cgggtcgctg
      481 ttggttgctg caagggggcg tggctcgacg gcgcggacgc gaccgcgatc gagtgcgccg
      541 aaggtagcgt gagacaagtc gcgggttttc agatgggcag agtgagaact tccatcctgg
      601 gggacctcga cccctacccc cgaccactca cctcgtgctc agcacaccct catttgcgaa
      661 gagccgctaa aagaggtcga caccgcggag cgagatcgtg cggctgagaa agcagtagga
      721 gggccctccg tagaggaccc tcctgggagt cggggtggcg ggattcgaac ccacggcctc
      781 gtcgtcccga acgacgcgcg ctaccaaact gcgccacacc ccgaagcact gtcaccaaca
      841 tgccggtgac aattgtacct gcctattaac ttatcttaac gggtcacagt gttcataaga
      901 ttcgaacctg cgacctcgtc gtcccgaacg acgcgcgcta ccaaactgcg ccacaccccg
      961 tgaagagcct gcttaggata tccgacctca ccctcgcagc gaaatcgccc tcctgccgcc
     1021 tactcgcgag cggtcaatgt gaggactgtt gcttcgggcc tgcatgcgaa tctcaccggg
     1081 gcaaatggcg atgtgcccgc gcccgcgctc acgtgaagcc acgcatcgtc cggccccaag
     1141 gcctcacccg gccaggtcga caaccctttg gcgcggcgtc ggtccagatc gcaatttgtc
     1201 accaaagcgc cgtatccggg gacgcacacc tgtccgccgt gagtgtgacc cgccaaaatc
     1261 aggtcagcgc cgtcggcctt gagcgcgtgc agcgcccgga ggtacggggc gtggacgaca
     1321 ccgaggtgca gtgttccgtg gtcgcggccg tccggcgggg gcatgcggtc gcgcccgatg
     1381 tgcggatcat ccatgcccac agtggacacg gcgaccccgc cgatgtcgag cgaggccctc
     1441 gcgttgttga ggtcatgcca accggcgtcc aggaacacgg atctcaactg gtcagtgggc
     1501 agccgtacct gttcttgaag agccctcgaa gggccggcga agtacccgag ggggttcttc
     1561 aggtgcggcg cgaagtagtc gttggacccg aagacgaaca ctccgggagc ggacaacact
     1621 cccgcgagcg cctccgcgac ggccgggacc gcatcggcgt gggcaagatt gtcgcccgtc
     1681 accaccacga ggtcgggctg ctcctgagcc agggaggccg cccacgcgat cttgtcgcgt
     1741 tgacgcgggg tcaggtgcaa gtcggacacg tgcaacaccc gtagcgaggc ctgccccggc
     1801 gcgagcaccg gaaccgtgac cctgcgggtg gtgaaggcgt gcgcctccgc gaggccccac
     1861 gcgacgcacg ccgccccggc tgctccgaca ctcagcgcag ccgccgtcgc tcgattcacg
     1921 aagtcctacg gcttcggatc gggggcgggg gtgggtgaag gcggcgctac aggagtgggg
     1981 tcccacccct tggagggttt gttcttggtc cagtcggccg gaggccaggc tccaccccac
     2041 cagtccgcgg gcgccgtgag cggatcccat ccggagggcc acttggtcca ccagccgggc
     2101 aacgcgtcgg tcgacatgta gaaggtgacc acgccgccgg ctctcatggt ccagtcggcc
     2161 gccggagatt gccgaacgat ctgcccagcg ggcactccag gcacgtacac caactcggct
     2221 gatttcgcat actggaaacc cgcatcgttg atcgcccatt cggcggcctg caccgacagt
     2281 ccgagcccga ccacgttggg cacgcgcaac tgagagccgt tgatcatcgc gtcggacgcc
     2341 cccggcagac ccacgcccgg gtagggcttc agtgcctgcg tcatgaagtc ctgccagacg
     2401 ggccccgaaa tgtctgcgcc gtaccagtac cacttgtgca ccccgttgat cgtgaaatct
     2461 tgcagtggaa cgtcgacttc ggcgttacct gtccatacgg ccgacaccac ctggggcgta
     2521 aagcccgcga accacatgtg ggcgttgttc tgtgacgtgc cggtcttgcc cgccacaggg
     2581 cggccgatcc caagatactt gcccgttccg gtctccatga cctgggtcag cgcataggcc
     2641 acgccatccg cgacttctgc cctgatcacc tgtgagcact tcacctccgg aggcacgatc
     2701 ggcttgccgt ccgggccgag gacctcggag ccatccagct tggtcatcga caggatcgcg
     2761 cggggctcgc agtgaatgcc gcggttggca aacgtctggt aggcggatgc catggtgagc
     2821 ggcgaggcgt tttgggaccc cagcgtcacc gagggcacca cctcaaagtc cagtccatcc
     2881 gagcgatgga agcccagggt cagcgccatg tccctgacct ggcacaggtc cagctgatgc
     2941 gtcatcgcca catatgcggt gttcactgac aaggtggtcg ccctgagcac cgacatctgg
     3001 acgccctcgg acgccgagac gttgccgacc ttgtagtcgc tgacatagcc ggcgccttcc
     3061 gggcaactgg tgcgccacgt ggggcccttc cacgtgcgcg gcacgcccga aatcacctgg
     3121 ctcagcgagt gtcctgagtc gagccactgc gatagcacca agggcttgaa ggacgagccc
     3181 ggcgagaatc cgcgagaacc gccataatcg cggtccaccg cgtagttgat cgcggtcgaa
     3241 tacggggctt tcgacgtggg gtcgaactcg cggttttgag acatcgcgag gatgttgccg
     3301 gtggtcacgt cga
//
LOCUS       DASRKH010000389         3546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_37777, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000389 DASRKH010000000
VERSION     DASRKH010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37777"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..394
                     /gene="rpsM"
                     /locus_tag="VFC06_06255"
     CDS             20..394
                     /gene="rpsM"
                     /locus_tag="VFC06_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HZL81520.1"
                     /translation="MARLAGVDLPREKRLEIALTYVYGMGRTRAKETLEATGVSPDLR
                     VKDMADSDLVALRDYIDANYKVEGDLRREVAADIRRKVEIGCYQGIRHRRGLPVHGQR
                     TKTNARTRKGPKRTVAGKKKAK"
     gene            454..861
                     /gene="rpsK"
                     /locus_tag="VFC06_06260"
     CDS             454..861
                     /gene="rpsK"
                     /locus_tag="VFC06_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008747488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HZL81521.1"
                     /translation="MPPKTRTTSSARKGRRKEKKNVAHGNAYIKSTFNNTIVSITDPT
                     GAVIAWASSGQVGFKGSRKSTPFAAQLAAEAAARRAQEHGMKKVDVFVKGPGSGRETA
                     IRSLQATGLEVGSISDVTPQAHNGCRPPKRRRV"
     gene            992..2005
                     /locus_tag="VFC06_06265"
     CDS             992..2005
                     /locus_tag="VFC06_06265"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HZL81522.1"
                     /translation="MLIAQRPTLTEDVVADYRSRFIIEPLEPGFGYTLGNSMRRTLLS
                     SIPGAAVTSIRIDGVLHEFSTIPGVKEDVAEIILNIKNIVVSSEQDEPVVMYLRKQGP
                     GVVTAGDITPPAGVEVHSPDLHIATINAKGKLEIELTVERGRGYVSAIQNKAVDAEIG
                     RIPVDSIYSPVLKVTYKVEATRVEQRTDFDRLIVDVETKPAIAPRDALASAGKTLVEL
                     FGLARELNVEAEGIEIGPSPTDAALAQDLALPIEDLGLLSRSYNCLKREGIHSVGELV
                     ARSEADLLDIRNFGAKSITEIKEKLASLGLSLKDSPADFDPSLHFGADLDYDGDVQFS
                     DER"
     gene            2027..2539
                     /gene="rplQ"
                     /locus_tag="VFC06_06270"
     CDS             2027..2539
                     /gene="rplQ"
                     /locus_tag="VFC06_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HZL81523.1"
                     /translation="MPTPSKGPRLGGSAAHERLMLSNLATALFEHKAITTTEHRAKRL
                     QPLAEKLISKAKRGDLHARRTVMTTVRRKDVVAELFEEIAPKFDGREGGYTRITKMPN
                     RKGDNAPMALIELVLEPVKAKKAPARKSAATKATAPVATVEETAADTTEVAEESAAAV
                     GDEAAQSVED"
     gene            complement(2635..3396)
                     /locus_tag="VFC06_06275"
     CDS             complement(2635..3396)
                     /locus_tag="VFC06_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HZL81524.1"
                     /translation="MRSDATLAIDCGGGGIKASVLDHSGTLHAQPVRATTPYPLPPER
                     LVSVIRSLADQLPRAQRATVGMPGMIRHGVVVATPHYVCRAGPRTKVLPELVSAWSGF
                     DMREAVEESLGMPALVLNDAEVHGCGLVTGVGLEMVITLGTGLGNAIFDNGVLAPHAE
                     LSRGTVKWGLTFDEYLGEHERIRLGDAHWSRRVRNVVESYQPVYLWDRLYIGGGNSRR
                     ITATVVARLGDRVVLANNAAGILGGVRAWDMVPQD"
     gene            3456..>3546
                     /locus_tag="VFC06_06280"
     CDS             3456..>3546
                     /locus_tag="VFC06_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019619031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HZL81525.1"
                     /translation="MPVRLRIDCAYDGTSFSGWARQPGLATVEG"
BASE COUNT          639 a         1256 c         1156 g          495 t
ORIGIN      
        1 ctcctaggag atagttacat tggcacgtct cgccggcgtt gacctccccc gcgaaaagcg
       61 gctggagatc gcgctcactt acgtctacgg catgggtcgc actcgtgcga aggagaccct
      121 cgaggccacc ggggtctccc cggacctgcg ggtcaaggac atggccgact ccgatctggt
      181 cgccctgcgc gactacatcg acgcgaacta caaggtcgag ggtgacctcc gccgcgaggt
      241 tgccgccgac atccgccgca aggtggagat cggctgctac cagggcatcc gccaccgtcg
      301 cggcctcccc gtgcacggcc agcgcaccaa gacgaacgca cgtacgcgca agggtccgaa
      361 gcggaccgtc gccggcaaga agaaggccaa gtagcccacc gcctgtccgc tgccacggcg
      421 tagcggatcg aagaccttag gagaaagtcg cacatgcccc ccaagactcg taccacgagc
      481 agcgctcgga agggccgtcg caaggaaaag aagaatgttg cccatggcaa cgcctacatc
      541 aagtcgacct tcaacaacac catcgtgtcc atcacggacc cgaccggtgc tgtgatcgcc
      601 tgggcctcat cgggccaggt cggtttcaag ggttcacgca agtcaacacc cttcgccgcg
      661 caactcgcgg ccgaggccgc ggcgcgcagg gcccaggagc acggcatgaa gaaggttgac
      721 gtgttcgtca agggacccgg ttccggccgc gaaaccgcga tccgctccct ccaggccacc
      781 ggcctcgagg tgggctcgat ctcggacgtc accccgcagg cccacaacgg ctgccggccc
      841 ccgaagcgtc gtcgcgtctg atcgcgacag gcggggtgga caccccgccg gacagatccc
      901 gctcgagatc gtcgagtccc cccgggtgga ccaaggcccg ggggtgtttg ccaggcgtca
      961 tatggcggat gccggctgaa aggaagacac agtgctcatc gcacagcgcc ccaccctcac
     1021 cgaggacgtc gttgccgact accggtcgcg gttcatcatc gagcccctcg agccggggtt
     1081 cggctacacc ctcgggaact cgatgcgccg cacgctgctc tcgtcgatcc cgggggcggc
     1141 cgtgacgtcg atccggatcg acggcgtgct ccacgagttc tccaccatcc ctggtgtgaa
     1201 ggaggacgtg gccgagatca tcctgaacat caagaacatc gtggtctcct ccgagcagga
     1261 cgagcccgtc gtgatgtacc tgcgcaagca gggcccgggt gtggtcactg ccggtgacat
     1321 cacgcccccg gcgggagtgg aggtccacag ccccgacctg cacatcgcga ccatcaacgc
     1381 gaaggggaag ctcgagatcg agctcacggt cgagcgtggc cgcggatacg tgtccgcgat
     1441 ccagaacaag gcggttgacg ccgagatcgg gcggatcccg gtcgactcga tctactcgcc
     1501 ggtcctcaag gtgacctaca aggtcgaggc cacccgcgtg gagcagcgca ccgacttcga
     1561 ccgcctcatc gtggacgtcg agaccaagcc cgccatcgcg ccccgtgacg cgcttgcatc
     1621 ggccggcaag acccttgtcg agctcttcgg gctcgcgcgc gagctgaacg tcgaggccga
     1681 gggaatcgag atcgggccct ccccgaccga cgccgcactc gcacaggacc tcgccctccc
     1741 catcgaggac ctgggcctgc tctcgcggtc gtacaactgc ctcaagcggg agggcatcca
     1801 ctccgtgggt gaactcgtcg cccgctcgga ggccgacctg ctggacatcc gcaacttcgg
     1861 cgcgaagtcc atcaccgaga tcaaggagaa gctggcgtcc ctcggcctgt ccctcaagga
     1921 ctcgcccgcg gacttcgacc cctcgctgca cttcggcgcc gacctggact acgacggcga
     1981 cgtccagttc agcgacgagc gctgagaaac atctgaagga gacatcatgc ccaccccctc
     2041 caagggacca cgtctgggtg gcagcgccgc gcacgagcgg ctcatgcttt ccaacctcgc
     2101 caccgcgctc ttcgagcaca aggcgatcac caccacggag caccgggcga agcggctcca
     2161 gccgctcgcc gagaagctca tcagcaaggc caagcggggt gacctgcacg cccgccgcac
     2221 ggtgatgacc acggtgcggc gcaaggacgt cgtcgccgaa ctcttcgagg agatcgcgcc
     2281 gaagttcgac ggccgcgaag gcgggtacac ccgcatcacc aagatgccga accgcaaggg
     2341 tgacaacgcc cccatggcgc tcatcgagct cgtcctcgag cccgtgaagg ccaagaaggc
     2401 cccggcaagg aagtccgccg ccaccaaggc caccgccccg gtcgccacag tggaggagac
     2461 agccgcggac accacggagg tggcggagga gtccgccgcc gcagtcggtg acgaggcagc
     2521 tcagtccgtg gaggactagc ggccgaccac caccgtcgtg ccatgagggg agttcccgcg
     2581 agggagctcc cctcaggtat tcccaccctc gcaggtctcg gccccagccg gccctcagtc
     2641 ctgcggcacc atgtcccagg cgcgaacgcc gccgagtatg ccggcggcgt tgttggcgag
     2701 taccacgcgg tccccgaggc gggccacgac ggtggccgtg atccggcggg agttgccgcc
     2761 gccgatgtac aggcggtccc acaggtagac aggttggtag gactccacca cgttgcgcac
     2821 gcggcgtgac cagtgggcgt cgcccaaccg gatgcgttcg tgctcgccga ggtactcgtc
     2881 gaacgtcagg ccccacttca ccgtgccgcg cgaaagctcg gcgtggggcg ccaggacgcc
     2941 gttgtcgaag atggcgttgc cgagcccggt ccccaaggtg atcaccatct ccagcccgac
     3001 gccggtgacc agcccgcagc cgtgcacctc ggcatcgttc agcacgagcg ccggcatccc
     3061 gagcgactcc tcgaccgcct cccgcatgtc gaaaccggac cacgccgaca ccagctcggg
     3121 gagcaccttc gtccgcggcc cggcccggca gacgtagtgc ggggtggcca ccacgacgcc
     3181 gtgccggatc atgccgggca tccccaccgt cgcgcgctgg gcacggggga gctggtcggc
     3241 gagggaacgg atcacggaga cgagccgctc ggggggcagc gggtagggcg ttgtggcccg
     3301 gaccggctgg gcgtgcagcg tacccgagtg gtccaggacg gaggccttga tgccgccgcc
     3361 tccgcagtcg atggcgaggg tggcgtcgga gcgcatgatc cgacgctagt gcatcggcat
     3421 ccccccgcaa ctgccggatg tggttgcatt gcctcatgcc cgtacgcctg cggatcgact
     3481 gcgcctacga tggcacgtcc ttctccggat gggcgcgcca gccggggctg gccacggtcg
     3541 aggggg
//
LOCUS       DASRKH010000390         2445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_56301, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000390 DASRKH010000000
VERSION     DASRKH010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56301"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VFC06_06285"
     CDS             complement(<1..278)
                     /locus_tag="VFC06_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015770914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81526.1"
                     /translation="MSRAGCHNRRVSEAHRSPFVEFDRDQWSALAASTPLPLTDADVV
                     RLRGLGDPIDLAEVDAVYRPLSSLLEMYVSATRGLHHATGAFLRGIAAR"
     gene            303..2162
                     /gene="glmS"
                     /locus_tag="VFC06_06290"
     CDS             303..2162
                     /gene="glmS"
                     /locus_tag="VFC06_06290"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HZL81527.1"
                     /translation="MCGIVGYVGPATPSSRPLDVVMEGLARLEYRGYDSAGVAVVTPG
                     GLKWAKRAGKLTNLSTALKEKPLPLATAAIGHTRWATHGGPTDTNAHPHISADGNLAL
                     IHNGIIENFNTLKDELAADGVSFLSETDTEVAAHLVSRAYNELGDLTAAMLAVTRVLE
                     GAFTLLAVHADRPGTVVGARRNSPLVVGLGEGENFLGSDVAAFISQTKSALELGQDQV
                     VTITADSVRVIDGDGNPVEPRAFTVDWDASAAVKGGFATFMDKEIHDQPKAVADTLLG
                     RADKSGHLALDELHISESVLRSIDKIVVIACGTAAYAGQVAKYAIEHWCRIPVEVELA
                     HEFRYRDPVVNEKTLVVAISQSGETMDTIMAVRHAREQGSKVLAIVNTHGSTIPRESD
                     AVLYTHAGPEVAVASTKAFLAQITACYLLGLYLAQLRGNMYADEIRSILKELALMPDK
                     IQRVVDNEEQVRDVARSMKDATSVLFLGRHVGFPVAMEGALKLKELAYIHAEGFAAGE
                     LKHGPIALVEQDQPVFVIVPSPRGRDQLHAKVISNIQEVRARGARTLVIAEEGDTAVE
                     PFADVVFRVPQTPTLLAPLVCVVPLQIFACELATAKGLDVDQPRNLAKSVTVE"
     gene            2162..>2445
                     /locus_tag="VFC06_06295"
     CDS             2162..>2445
                     /locus_tag="VFC06_06295"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013601780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HZL81528.1"
                     /translation="MIRGIGVDITDVARLGAALERVPRLRERLFTPGERDLPLRSLAA
                     RFAAKEAVAKALGSPGGMSWQDCEVVLDHTGAPSLRVRGSVARVAAARGIT"
BASE COUNT          403 a          865 c          833 g          344 t
ORIGIN      
        1 cgcgccgcga taccgcgcag gaaggccccg gtggcgtggt gcaggccgcg cgtggcagag
       61 acgtacatct cgaggagcga ggacaacggc cggtagaccg cgtccacctc cgccaggtcg
      121 atcgggtcac cgagaccgcg caaccgtacg acgtcggcgt cggtcagcgg caggggcgtc
      181 gaggccgcga gcgccgacca ctggtcacgg tcgaactcga cgaaggggga gcggtgcgcc
      241 tcggacaccc gtcgattgtg acatcccgcg cggctcacgg tgtggcaact agactggccc
      301 ccatgtgtgg aatcgttgga tacgtcggcc ctgccactcc ttcgagccgt cccctggacg
      361 tggtgatgga aggactcgcg cggctcgagt accgtggcta cgactccgcg ggcgtcgcgg
      421 tcgtcacgcc cgggggcctg aaatgggcga agcgcgccgg caagctcacc aatctctcga
      481 ccgcgctgaa ggaaaagccc ctcccgctcg cgaccgcggc gatcggccac acgcggtggg
      541 caacgcacgg cggccccacg gacaccaacg cgcacccgca catctccgcg gacggcaacc
      601 tcgcgctgat ccacaacggc atcatcgaga acttcaacac cctcaaggac gaactcgccg
      661 ccgacggcgt ctccttcctc tccgagacgg acacggaggt ggccgcacac ctggtgtcac
      721 gcgcctacaa cgagctcggc gacctgaccg ccgccatgct cgccgtgacc cgcgtgctcg
      781 agggcgcgtt caccctcctc gcggtgcatg cggaccggcc cggcacggtg gtcggggcgc
      841 ggcgcaactc accgctcgtc gtcggcctcg gcgaggggga gaacttcctc ggctcggacg
      901 tcgccgcgtt catctcccag accaagtcgg cgctggaact cggccaggac caggtggtca
      961 ccatcacggc ggactcggtg cgggtgatcg acggcgacgg caaccccgtc gagccgcgtg
     1021 cgttcaccgt ggactgggac gcgtcggcgg cggtcaaggg cggtttcgcc accttcatgg
     1081 acaaggagat ccacgaccag ccgaaggccg ttgcggacac cctcctcggg cgggccgaca
     1141 agagcgggca cctggcgctc gacgaactgc acatcagcga gtcggtgctg cggtcgatcg
     1201 acaagatcgt ggtgatcgcc tgcggcaccg ccgcctacgc cggacaggtg gcgaagtacg
     1261 ccatcgagca ctggtgccgc atccccgtgg aggtggagct cgcacacgag ttccgctacc
     1321 gcgacccggt ggtcaacgag aagaccctgg tggtggcgat cagccagtcc ggggagacga
     1381 tggacaccat catggcggtg cgccacgccc gcgagcaggg ctcgaaggtg ctcgccatcg
     1441 tgaacaccca cggatcgacg atcccgcgcg agtccgatgc ggtcctgtac acgcacgccg
     1501 gccccgaggt tgccgtggcc tccaccaagg cgttcctcgc ccagatcacg gcctgctacc
     1561 tcctcggcct gtacctggcg cagctgcgcg gcaacatgta cgccgacgag atccgctcca
     1621 tcctgaagga actcgccctc atgccggaca agatccagcg cgtggtcgac aacgaggagc
     1681 aggtgcggga cgtggcccgc agcatgaagg acgccacgag cgtgctcttc ctcggccggc
     1741 acgtcgggtt ccccgttgcg atggagggcg cgctgaagct caaggaactc gcgtacatcc
     1801 acgccgaggg attcgcggca ggtgagctga agcacgggcc gatcgccctc gtggagcagg
     1861 accagcccgt cttcgtgatc gtcccctccc cgcgggggcg cgaccaactg cacgcgaagg
     1921 tcatctccaa catccaggag gtccgggccc gcggcgcccg cacgctggtg atcgccgagg
     1981 agggggacac ggccgtcgag cccttcgcgg atgtggtgtt ccgcgtgccg cagacgccaa
     2041 cactcctcgc cccgctggtg tgtgtggtgc ccctgcagat ctttgcgtgc gagctagcca
     2101 cggccaaggg tctcgacgtg gaccagcccc gcaacctcgc gaaatcggtc acggtggagt
     2161 gatgatcagg ggcatcggcg tggacatcac cgacgtcgcc cgcctcggcg ccgccctcga
     2221 acgcgttccc cgcctgcggg agcgactctt cacccccggt gagcgcgacc tgcccctccg
     2281 ttccctcgcc gcccgcttcg cggccaagga agcggtggcg aaggccctgg gctcgcccgg
     2341 tggcatgagc tggcaggact gcgaggtggt ccttgaccat accggggcgc cgagcctgcg
     2401 ggtgcgcggg agcgtggcac gggttgccgc cgcgcggggc atcac
//
LOCUS       DASRKH010000391         3008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_33625, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000391 DASRKH010000000
VERSION     DASRKH010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33625"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VFC06_06300"
     CDS             complement(<1..464)
                     /locus_tag="VFC06_06300"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048641.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HZL81529.1"
                     /translation="MTAIDTKERRSSSSLHVGRVARVIGPIVDIEFPPDAIPEMYNAL
                     KTTIDLSTQGEGESVIEMTLEVAQHLGDNMVRAIALKPTDGLVRGARVIDTGGPISVP
                     VGDVTKGHVFNVTGDCLNLKEGEVLDIKERWPIHRKPPAFDQLESKTQMFETG"
     gene            complement(485..1429)
                     /locus_tag="VFC06_06305"
     CDS             complement(485..1429)
                     /locus_tag="VFC06_06305"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015881805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HZL81530.1"
                     /translation="MGGKQRVYKNRIKSTQTLKKVFRAMELIAASRIGRARTRAIQGT
                     PYANTLAKAVSAVVSHLRAEHPLLKERHDTNRVAILVVTSDRGMAGAYSASVLREAEH
                     LMESLESDGKVPVLYVVGRRGASYFNFRGREVARVWDGHSDSPKAKEATEIADLLRQV
                     YHSSGPDAVCEVYTISTHFVTMVRQVVEVHRLLPIEVIDTVQVPETSPLYPLAVEKRL
                     KGAADPEPLALYEFEPNADEVLAALLPLHARARVHNMLLHAAASELASRQRAMHTATD
                     NAEDLIRNYTRLANSARQAEITQEISEIVSGADALASS"
     gene            complement(1432..2865)
                     /gene="atpA"
                     /locus_tag="VFC06_06310"
                     /pseudo
     CDS             complement(1432..2865)
                     /gene="atpA"
                     /locus_tag="VFC06_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019134872.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
     gene            2908..>3008
                     /locus_tag="VFC06_06315"
     CDS             2908..>3008
                     /locus_tag="VFC06_06315"
                     /inference="COORDINATES: protein motif:HMM:NF014873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81531.1"
                     /translation="MGVVVLGEFTGIEEGQEVRRTGEVLSVPVGDGYL"
BASE COUNT          439 a          985 c         1047 g          537 t
ORIGIN      
        1 ccggtctcga acatctgggt cttggactcc agctggtcga atgcgggtgg cttccggtgg
       61 atcggccaac gctccttgat atcgaggacc tcgccctcct tgaggttgag gcagtcgccg
      121 gtgacgttga acacgtggcc cttggtcacg tcgccgaccg gaaccgagat cgggccgccg
      181 gtgtcgatga cccgggctcc gcggacgagg ccgtcggtcg gcttgagtgc aatggcgcgc
      241 accatgttgt ccccgaggtg ctgcgccacc tcgagggtca tctcgatcac cgactcgccc
      301 tcaccctggg tggagaggtc gatcgtcgtc ttcagggcgt tgtacatctc cgggatggcg
      361 tccgggggga actcgatgtc cacgatcggc ccgatcacgc gcgcgacccg gccaacgtgg
      421 aggctcgagg acgagcgccg ctccttggtg tcaatggcag tcattgctct tccttccctt
      481 gaacctagct cgaagccagc gcgtcggcac cggacacgat ttcggagatc tcctgggtga
      541 tttccgcctg gcgcgcggag ttcgccagcc gggtgtagtt gcgaatcagg tcttccgcgt
      601 tgtccgtcgc ggtgtgcatg gcacgctggc gggacgcgag ctccgaggcg gcggcgtgca
      661 gcagcatgtt gtggacccgg gcacgggcgt gcagcgggag cagtgctgcc agcacctcgt
      721 cggcgttggg ctcgaactcg tacagtgcga ggggttccgg atccgctgca cccttcagcc
      781 gcttctcgac cgcgagaggg tacagtgggg aggtctcggg cacctgcacg gtgtcgatga
      841 cctcgatcgg gaggagtcgg tgcacctcga cgacctgacg gaccatcgtc acgaagtggg
      901 ttgagatcgt gtacacctcg cagacggcgt ccggaccact ggagtggtac acctgacgga
      961 gcagatcggc gatctcggtt gcttccttgg ccttcgggga gtcggagtgc ccgtcccaga
     1021 cccgcgcgac ctcccggccg cggaagttga agtacgacgc cccgcgccgt ccgaccacgt
     1081 agaggacggg caccttgcca tccgactcca gcgactccat caggtgttcg gcctcccgca
     1141 gcacgctggc gctgtaggcg ccggccatgc cgcggtccga ggtcacgacc aggatggcga
     1201 ccctgttggt gtcatggcgc tccttgagga gagggtgttc ggccctgagg tggctgacga
     1261 ccgcggacac ggccttggcg agcgtgttgg catacggagt cccctggatc gcgcgtgtgc
     1321 gcgcccggcc gatgcgcgac gcggcgatca gctccatcgc gcggaacacc ttcttcaggg
     1381 tctgcgtcga cttgatccgg ttcttgtaga cgcgctgctt gccgcccatg ctcagccccg
     1441 cttggagcga acgatttgct cccgggtctc gatgacctcg tgctcctcgt cgggcagcgt
     1501 ggccgtcgcg tcgagcggga ggccggcctt ggtcaggaac ccacgccgga aggcctcgac
     1561 ggctttccgc agggcttcct ccgtgtcctt ctcgagccgc cccgtctggt tgatggtgtc
     1621 cagcaccgtg ctggtgcgcc ggaggtggtc gaggagttcg agctcgaagc gggtgatgtc
     1681 ctcgagatcc acgtcgtcga cgtagccgtt ggtccccgcc cagatcacgg ccacctgcga
     1741 gcccacctcg tagggcgtgt actgcggctg gcgcagcaac cgcatcaggc gcgccccgcg
     1801 tgtcaactgc tgccgtgtgg cggcgtcgag gtccgaggcg aacatcgcga aggcctccat
     1861 cgaccggtac tgggcgaggg tgatcttgag cgtgcccgcc acctgcttca tggccttcac
     1921 ctgggcggcg ccgccgacgc gggacaccga gatgccgacg tccaccgccg gacgctggtt
     1981 ggcgttgaag aggtcggact ggaggaagat ctggccgtcg gtgatggaga tgacgttggt
     2041 gggaatgtac gccgagacgt cgttcgcctt ggtctcgatg atcgggaggc ccgtcatcga
     2101 gccggcaccc agttcgtcgg acagcttcgc gcaacgctcc agcaggcggg agtgcaggta
     2161 gaacacgtcg cccgggtacg cctcgcggcc cggcggccgg cgcagcagga gcgagacggc
     2221 ccggtaggcc tccgcctgct tggagaggtc gtcgaagatg atcaggacgt gcatcccctg
     2281 gtacatccag tgctggccga tcgcggaacc ggtgtacggc gcgaggtact tgtagccagc
     2341 ggggtccgac gcgggggcag cgatgatcgt ggtgtacgga agtgcgcccc cgtcctcgat
     2401 cgcggcacgc acggacgcga tcgtcgagcc cttctggccg atcgcgacgt agatgcagcg
     2461 cacctgcttg gtcttgtcgc ccgactccca gttggccttc tggttgagga tggtgtcgat
     2521 cgcgatggcg gtcttgcccg tctggcggtc accgatgatc agctgccgct ggccgcgacc
     2581 gaccgggatc atggagtcga tggccttgag gcccgtctgc agcggctcgt gcacggactt
     2641 gcgcatcatc acgccggggg cctgcagctc gagcgcgcgc cgcccgtcga ttcccacgat
     2701 ctcgccgagg ccgtcgatcg ggtggcccag agggtccacg acccggccga ggtacccgtc
     2761 gcccaccggg accgagagca cctcaccggt ccggcgcacc tcctgaccct cctcgatacc
     2821 ggagaactcg ccgagcacca cgacgccgat ctcgcgcacg tcgaggacgg gacgctcggc
     2881 cttgcgctga acctcgacgt ccgcgagatc ggcgtcgtcg tgctcggtga gttcaccggc
     2941 atcgaggagg gccaggaggt ccgccggacc ggcgaggtcc tctcggtccc cgtcggcgac
     3001 ggctacct
//
LOCUS       DASRKH010000392         5047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_51840, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000392 DASRKH010000000
VERSION     DASRKH010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5047
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51840"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1244
                     /locus_tag="VFC06_06320"
     CDS             <1..1244
                     /locus_tag="VFC06_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-5'-phosphate-dependent protein subunit
                     beta"
                     /protein_id="HZL81532.1"
                     /translation="VLSRELTGIDSPIIVLLGNRFPMITSHKVMAAFGCLAPRLVSGQ
                     FDPTRHRAIWPSTGNYARGGVAISRLMACRGVAILPTGMSQERFDWLDRWCEDPSEDI
                     IRTVGTESNVKEIYDECNRLARDPGNFIFNQFAEFGNHLVHFQATGAALAHVFETYRE
                     SSGRDARLALMTSATGSAGTIAAGEYLKDRYGTKVLAAEALECPTLLENGFGEHNIQG
                     IGDKHVPLIHNIMNHDLVAAISDRATDRLEVMFNEPEGQAHLAGKGVPQHILDALPDF
                     GFSSICNVLAAISAAKVLGLDEDDVIMTVATDGGQMYPSERARILQRDFQGEFTRLDA
                     AEVWGEHLANVPTDATLEMTERDRNRIFNLGYYTWVEQQGTPFELFERRRGQDFWREL
                     RAYLPLWDEMITDFNARVAAS"
     gene            1360..4446
                     /gene="ygfK"
                     /locus_tag="VFC06_06325"
     CDS             1360..4446
                     /gene="ygfK"
                     /locus_tag="VFC06_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HZL81533.1"
                     /translation="MGDLMRPLPFGVLLEWALAEYHVHGSIFGLGAEHFFVPAEGRAI
                     RDPMGHAIATPIGPAAGPHTQLAPNIVVAYLAGARFIELKTVQVMDGEAIRAAVAKPC
                     INAEDEGFNCEWSTELTVGQAFDEYVRAHLAIAVLAVELGLSDGDDVAYNASVGYDLA
                     GVSTPLIDGFIEGLRDASATPVYRDAVAWLGAHLDRFTRFGRSHLERLSPHICDSITL
                     STLHGCPADEIERIANHLLDKGLHTFVKANPTMLGYDAARGILDGLGYSHITFDDHHF
                     VGDLQFDDAVAMFGRLAAKAEAAGLVFGVKLSNTLPNDVTRGELPAEEMYMSGRALAP
                     LTLALAAKLSHAFDGRLPISYSGGADATNIEAILATGIQPVTVATTLLKPGGYARLHQ
                     MAHLASGAMRDHDRVDVAALDALEARVIADERIHKRHREKVGSRKTASPLPLTDCFTA
                     PCEHGGCPIEQQIPEYLTLVAAERYAEAFAEIARDNTAPTITGVLCSQPCREHCTRLD
                     YDTSIDMRGVKLAAADAAQEAFIAATTPAPLVTSARVAVIGAGPAGIAAALFLRRNGV
                     DVEVFEKLDGPYGIVRYIIPTFRITREQIDRDFRLAVAAGVVFHFGCDPAYELVQLRA
                     RFDDVVVATGAWGRGRSPVVTGGEHVVDALGFLWEHVNGSGAHLGRRVAVVGAGDVAM
                     DCARTALRTPGVEHVTVVYRRTEPFMPAAQEDVNIVRAEGIEILELLSPISYDGGVLR
                     CERTQLGDRDASGRRTTRGTGAYVDLEFDDVIDATGAVIDPERYTANGIAVDERGRPR
                     VDATFEAEVAGGLTGVYVVGDGRLGTHTIVRAIADAKVAARAILATHGVRADFDHSPA
                     AVFTPLAVLAGRRGVLRDRLAPPAEGTRCLRCDEVCEICTEVCPNRANVAIAVPGFTD
                     PRQIVHLDGLCNECGNCGTFCPHAGLPYRDKITVFWTREDFEDSGNTGFLALPPDVDD
                     GAAYLVRLPSGEVRTHLSGADDLPADLGRVLAVLEAEHPYCLRAGLEPHELEGAAP"
     gene            4443..>5047
                     /locus_tag="VFC06_06330"
     CDS             4443..>5047
                     /locus_tag="VFC06_06330"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZL81534.1"
                     /translation="MIVTGGPVITGDPTQPVVTGGGVLVRDGVVEAVGPAADLRAAHP
                     AEPVTDTGRVILPGFINAHTHAYSAYARGMAVHSPTRDFPEILANLWWSLDRLLEPED
                     VRLNATTTFMESVRNGVTTVFDHHSSPHAIPGSLATMAHAARQVGLRACLCYETSDRD
                     GGAAFAAAVAENVDFMREANTGGQDAVRGMFGMHASFTLSEA"
BASE COUNT          767 a         1907 c         1657 g          716 t
ORIGIN      
        1 tcgtcctctc ccgcgaactc accggcatcg acagccccat catcgtcctg ctcggcaacc
       61 ggttcccgat gatcacctcc cacaaggtga tggccgcgtt cggctgcctg gcgccgcgcc
      121 tggtttcagg ccagttcgac cccacgcggc acagggccat ctggccgtcc accggcaact
      181 atgcccgcgg gggcgtcgcg atcagccgcc tcatggcctg ccggggcgtg gcgatcctgc
      241 ccaccggtat gagccaggag cgcttcgact ggctggaccg ctggtgtgag gacccctccg
      301 aggacatcat ccgcaccgtc ggcaccgagt ccaacgtcaa ggagatctac gacgagtgca
      361 accggctcgc gcgagatccg ggcaacttca tcttcaatca gttcgccgaa ttcggaaacc
      421 acctcgtcca cttccaggcc accggggccg cgctcgcgca cgtgttcgag acctaccgcg
      481 agagctcggg ccgggatgcg cggctcgccc tcatgaccag cgcgaccggg tcggccggca
      541 ccatcgccgc cggggagtac ctgaaggacc gctacggcac gaaggtgctg gccgcggagg
      601 ccctcgagtg ccccacgctg ctcgagaacg gcttcgggga gcacaacatc cagggcatcg
      661 gggacaagca cgtgcccctc atccacaaca tcatgaacca cgaccttgtc gcggcgatca
      721 gcgaccgcgc gaccgaccga ctcgaggtca tgttcaacga acccgagggg caggcccacc
      781 tggccggcaa gggcgtgccg cagcacatcc tggacgcgct gcccgacttc ggtttctcct
      841 ccatctgcaa cgtgctcgcg gcgatctcgg ccgccaaggt cctcggtctc gacgaggacg
      901 acgtgatcat gacggtggcc accgacggcg gccagatgta cccctccgaa cgcgcgcgga
      961 tcctccagcg cgatttccag ggcgagttca cccggctgga cgccgccgag gtctggggcg
     1021 agcacctcgc caacgtcccg accgacgcca cgctcgagat gaccgagcgc gaccggaacc
     1081 gcatcttcaa cctcggctac tacacctggg tggaacagca gggcactccg ttcgaactgt
     1141 tcgagcgccg ccgcggtcag gacttctggc gggaactacg ggcctacctg ccgctctggg
     1201 acgagatgat caccgacttc aacgcccggg tcgcagccag ctgacctgcg gctccgcgca
     1261 cgtgatgcgc caactttccc ggccaagtcc ctcgacagtg gccgccggaa gggggacgat
     1321 cgagagtatg gaacgccaca ccgtcgtggg actgtagtca tgggcgacct gatgcgtccg
     1381 ctgcccttcg gcgttctgct cgagtgggcg ctcgccgagt accacgtcca cggctccatc
     1441 ttcgggctgg gagcggaaca cttcttcgtt ccagccgagg gccgcgcgat ccgcgacccc
     1501 atgggccacg ccatcgccac gcccatcgga cccgccgcgg gcccgcacac ccagctggcg
     1561 cccaacatcg tggtggccta tctggccggc gcccgattca tcgagctgaa gaccgtccag
     1621 gtcatggacg gcgaggccat ccgcgccgcc gtcgcgaagc catgcatcaa cgccgaggac
     1681 gagggcttca actgcgagtg gtccaccgag ctcaccgtgg gccaggcgtt cgacgagtac
     1741 gtccgcgccc acctcgcgat cgccgtcctg gcagtggaac tcggcctctc ggacggcgac
     1801 gacgtcgcgt acaacgcgtc ggtcggctac gacctcgccg gcgtcagcac gccgctgatc
     1861 gacggcttca tcgagggcct gcgcgacgcc tccgccaccc cggtgtaccg cgacgccgtc
     1921 gcctggctgg gggcgcacct cgaccgcttc acccgcttcg gccgatccca cctcgagcgg
     1981 ctctcaccgc acatctgcga ctcgatcacc ctctcgacgc tgcacggctg ccccgcggac
     2041 gagatcgagc gcatcgcgaa ccacctcctc gacaaggggc tgcacacatt cgtgaaggcc
     2101 aatcccacaa tgctcggcta cgacgccgcc cgcggcatcc tcgacggcct cggctactcc
     2161 cacatcacct tcgacgacca ccatttcgtc ggcgaccttc aattcgacga cgccgtcgcg
     2221 atgttcggcc ggctggccgc gaaggcggag gcggccgggc tggtgttcgg cgtgaagctg
     2281 tccaacaccc tgcccaacga cgtcacccgc ggcgaactgc ccgccgagga gatgtacatg
     2341 tccgggcggg cgctggcccc gctcacgctc gcgctggccg cgaagctctc ccacgcgttc
     2401 gacggccgcc tgcccatctc ctattcgggc ggcgcggacg ccacgaacat cgaggccatc
     2461 ctggcgaccg gtatccagcc agtcaccgtc gccaccaccc tgctgaagcc gggcggctac
     2521 gcgcgcctcc accagatggc gcacctcgcc tccggtgcga tgcgggacca cgaccgcgtc
     2581 gacgtcgccg ccctggacgc gctggaggcg cgagtcatcg cggacgagcg gatccacaag
     2641 cggcaccgcg agaaggtcgg ctcgcggaag accgcctcgc ccctgcccct caccgactgc
     2701 ttcacggccc cctgcgagca cggcggctgc ccgatcgagc agcagatccc ggagtacctc
     2761 acgctggtgg cggccgagcg ttacgccgag gcgttcgccg agatcgcgcg ggacaacacc
     2821 gcccccacca tcaccggagt gctgtgctcg cagccgtgcc gggagcactg cacgcgcctg
     2881 gactacgaca cgtccatcga catgcgcggg gtgaaactcg ccgccgcgga cgccgcccag
     2941 gaggcattca tcgcggccac caccccggcc ccgctggtga ccagcgcacg ggtggcggtg
     3001 atcggcgccg gccccgctgg catcgcggcg gccctgttcc tgcgccgcaa cggcgtggac
     3061 gtggaggtct tcgagaagct ggacgggccg tacggcatcg tgcggtacat catcccgacg
     3121 ttccggatca cccgggagca gatcgatcgc gacttccggc tggccgtcgc cgccggggtg
     3181 gtgttccact tcggctgcga ccccgcctac gagctggtcc aactgcgcgc ccggttcgac
     3241 gacgtcgtcg tggccacggg cgcgtggggc cggggcagga gccccgtcgt caccggtggc
     3301 gagcacgtcg tcgacgccct gggtttcctc tgggagcacg tcaacggcag cggggcgcac
     3361 ctcggccggc gggtcgcggt ggtcggggcg ggcgacgtcg ccatggactg cgcgcgcacc
     3421 gcactgcgga cgccgggcgt ggagcacgtc acggtggtct accgccgcac ggagccgttc
     3481 atgccggccg cccaggagga tgtcaacatc gtccgggccg agggtatcga gatcctcgag
     3541 ctgctctcgc ccatctccta cgacggcggc gtgttgcgct gcgagcgcac ccaactcggg
     3601 gaccgtgacg cctccgggcg gcggaccacc cggggcaccg gcgcgtacgt ggacctcgaa
     3661 ttcgacgacg tcatcgacgc caccggcgcg gtgatcgacc cggagcggta cacggcgaac
     3721 gggatcgccg tcgacgagcg cggacgcccc cgcgtggatg cgacgttcga ggcggaggtc
     3781 gcgggcggcc tgaccggggt gtacgtcgtc ggcgacgggc gcctcggcac ccacacgatc
     3841 gtgcgggcca tcgcggacgc gaaggtcgcg gcccgcgcga tcctcgccac gcacggggtc
     3901 cgggccgact tcgaccactc ccccgccgcg gtgttcaccc cgctggcggt gttggcgggc
     3961 cggcgcggcg tcctgcgcga tcgcctcgcc ccacccgccg agggcacccg gtgcctgcgt
     4021 tgcgacgagg tgtgcgagat ctgcaccgag gtctgcccga accgggccaa cgtggcgatc
     4081 gcggtgccgg gtttcaccga cccgcggcag atcgtccacc tcgacgggct ctgcaacgag
     4141 tgcggcaact gcggcacgtt ctgcccgcac gccggcctcc cctaccggga caagatcacc
     4201 gtgttctgga cccgggagga cttcgaggac tccggcaaca cgggcttcct cgccctgccg
     4261 ccggacgtcg acgacggcgc cgcctacctc gtccgcctgc cctccggtga ggtccgaacc
     4321 cacctctccg gggcggacga cctgcccgcc gacctcggcc gcgtcctcgc cgtcctcgag
     4381 gcggagcacc cgtactgcct gcgggcgggc ctggagcccc acgagctgga aggagccgca
     4441 ccatgatcgt caccggaggt cccgtcatca cgggcgaccc cacccagccg gtggtcaccg
     4501 ggggtggagt gctggtgcgc gacggcgtcg tcgaggcggt cggtccagcg gccgacctgc
     4561 gggcggcgca ccccgccgag ccggtgaccg acaccgggcg tgtcatcctg ccgggcttca
     4621 tcaacgccca cacgcacgcg tacagcgcgt atgcgcgcgg catggccgtc cactccccca
     4681 cccgggactt ccccgagatc ctcgcgaacc tgtggtggtc cctcgaccgg ctcctcgagc
     4741 ccgaggacgt ccggctgaac gcgacgacca ccttcatgga gtcggtccgc aacggtgtga
     4801 ccacggtgtt cgaccaccat tccagcccac acgcgatccc cggctcgctg gccacgatgg
     4861 cccacgccgc ccgccaggtg ggcctgcgcg cgtgcctctg ctacgagacc tcggaccgcg
     4921 acggcggggc cgccttcgcc gcggcggtcg cggagaacgt ggacttcatg cgcgaggcca
     4981 acaccggggg ccaggacgcg gtgcgcggca tgttcggcat gcacgcctcg ttcacgctct
     5041 ccgaggc
//
LOCUS       DASRKH010000393         2711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_57210, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000393 DASRKH010000000
VERSION     DASRKH010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57210"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..930
                     /locus_tag="VFC06_06335"
     CDS             37..930
                     /locus_tag="VFC06_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HZL81535.1"
                     /translation="MPPEDARVLAPAKPAGPDAFEVTLTNFTGPFELLLTLITKHRLD
                     ITEVALAEVTDEFLSYMSGLEEWDLSRASEFLVVAATLLDLKAARLLPRGDVESEEDI
                     ELLEARDLLFARLLAYKAFKEVADHFAGLLEASARRLPRSVPLEPEFAALLPELVMSI
                     GSEGLAALAARALEPKPGLPAVELAHVYNPTVSVHDEGETVMTRLRRHHVLAFRALVA
                     DAASTAVVVARFLSLLELFHEGAVTFEQAEPLGELQVRWSGGEGEYTVGDDYDDAPAD
                     DDDAWAGGAHTVADEGEEAHE"
     gene            923..1519
                     /gene="scpB"
                     /locus_tag="VFC06_06340"
     CDS             923..1519
                     /gene="scpB"
                     /locus_tag="VFC06_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HZL81536.1"
                     /translation="MSDSYEPVPDDAVPERQLAALEAILMVVDEPIPAASLAEALGMP
                     TGQVVELLGALAADYRGQTGRPRGFDLREVAGGWRIYSAPEFADLVGRFVVSGQTARL
                     THAALETMAVIAYKQPISRGQISAIRGVSVDSVVRTLLARGLVKEAGQDEPTGALLYG
                     TTSYFLERLGLGSLDDLPPLAPYLPRLEALGEVDEEYR"
     gene            1516..2247
                     /locus_tag="VFC06_06345"
     CDS             1516..2247
                     /locus_tag="VFC06_06345"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HZL81537.1"
                     /translation="MKDDGERLQKVLAHAGIASRRVCEEMIADGRVEVNGEIVTEMGR
                     RVDPAVDVIHVDGSRVVLDADHLTIAFNKPTGVVSTMSDPEGRPSLGDFTSRYNTRLY
                     HVGRLDTPTQGLILLTNDGELAQRLAHPKYEVPKTYLATVNGRVPRGLVKKLKQGVEL
                     EDGVARADAVTVKETAAMASIIEIVLHDGRNRIVRRMMDAVGHPVIELVRTQVGPIRL
                     GDLRSGRSRIITGNELGTLMRTVGM"
     gene            2270..>2711
                     /locus_tag="VFC06_06350"
     CDS             2270..>2711
                     /locus_tag="VFC06_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HZL81538.1"
                     /translation="MAKSADVRGPVLVVGTGLLGASIGLALRELGVEVGLQDISPAAL
                     HLARDLGAGTPAGPDFHPALVVVATPPDVIVPAVVAALRDHPDAVVTDVASVKGSLAG
                     RVGEQVGAAVAQYVGSHPMAGRERSGPAAADSDLFQGRTWVICPH"
BASE COUNT          389 a          965 c          973 g          384 t
ORIGIN      
        1 accgtcgcct cgcccgtgag ctggtggcgc gcggcgatgc cgcctgagga cgcgcgcgtc
       61 ctcgcccccg ccaaacccgc cggcccggac gcgttcgagg tcaccctcac caacttcacg
      121 gggcccttcg aactgctgct cacgctgatc accaagcacc ggctggacat caccgaggtg
      181 gccctcgccg aggtcacgga cgagttcctc tcctacatgt ccggactgga ggagtgggac
      241 ctgtcccggg cgagcgagtt cctggtggtg gcggccacgc tccttgacct caaggccgcg
      301 cggctcctgc cgcgagggga cgtggagtcc gaggaggaca tcgaactgct cgaggcgcgg
      361 gacctcctct tcgcccgact gctggcgtac aaggcattca aggaggtcgc ggaccacttc
      421 gccggcctgc tcgaggcctc ggcccgcagg ctaccccggt ccgtgccgct cgaacccgag
      481 ttcgccgcgc tgcttcctga actggtgatg tcgatcgggt cggaagggct ggcggccctc
      541 gccgctcgcg ccctcgagcc gaagccgggc ctgcccgccg tcgagctcgc ccacgtgtac
      601 aacccgaccg tgtccgtgca cgacgagggc gagaccgtca tgacccgcct gcgccgccac
      661 cacgtgctgg cgttccgcgc gctcgtcgcc gacgccgcca gcaccgccgt cgtcgtcgca
      721 cggttcctct cgctgctcga gttgttccac gagggtgccg tgacgttcga gcaggccgag
      781 ccgctcggcg agctgcaggt gcgctggtcc gggggcgagg gtgagtacac cgtgggcgat
      841 gactacgacg acgccccggc ggacgacgac gacgcgtggg cgggcggcgc ccacaccgtc
      901 gccgacgaag gcgaggaggc ccatgagtga ctcgtacgag ccggtgcccg acgacgccgt
      961 ccccgagcgg caactcgcgg ccctcgaggc catcctgatg gtggtcgacg agcccatccc
     1021 ggcggcgtcg ctcgcggagg cgctcgggat gccgaccggg caggtggtgg agctcctcgg
     1081 ggccctcgcg gcggactacc gcgggcagac cgggcgcccg cgcgggttcg acctgcgcga
     1141 ggtggccggg ggctggcgga tctactccgc acccgagttc gcggacttgg tgggccggtt
     1201 cgtcgtctcc ggccagaccg cccgcctcac ccacgcggcc ctggagacga tggcggtgat
     1261 cgcctacaag cagccgatct cccgcggcca gatctcggcg atccgcggcg tcagcgtgga
     1321 ctcggtggtg cgcacgctgc tcgctcgcgg gctggtgaag gaggccgggc aggacgaacc
     1381 caccggcgcg ctcctctacg gcaccacgtc atacttcctc gagcgactcg gattggggtc
     1441 gctcgatgac ctgccaccat tggcaccgta cctgccgcgg ctggaagcgc tcggcgaggt
     1501 tgatgaggag tacagatgaa ggacgacggc gaacgcctgc agaaggtcct cgcccacgcc
     1561 gggatcgcat cccggcgggt gtgcgaggag atgatcgccg acgggcgcgt ggaggtgaac
     1621 ggcgagatcg tcacggagat gggccggcgg gtggaccccg ccgtggacgt catccacgtg
     1681 gacggctccc gggtggtgct ggacgccgac cacctgacga tcgcgttcaa caagcccacc
     1741 ggcgtggtct ccacgatgag tgaccccgag ggccgcccga gcctggggga cttcaccagc
     1801 cgctacaaca cgcggctcta ccacgtgggc cggctggaca cgcccaccca gggcctgatc
     1861 ctgctgacca acgacggcga actggcccag cgcctcgccc acccgaagta cgaggtcccc
     1921 aagacgtacc tggccacggt caacgggcgg gtcccgcgcg gcctcgtcaa gaagctgaag
     1981 cagggcgtgg agttggagga cggcgtggcg cgggccgacg ccgtgaccgt gaaggagacc
     2041 gccgccatgg cctccatcat cgagatcgtg ctccacgacg gccgcaaccg gatcgtgcgc
     2101 cgcatgatgg acgcggtggg ccacccggtc atcgagctgg tgcgcaccca ggtggggccg
     2161 atcaggctcg gggacctgcg ctcgggacgc tcacgcatca tcaccggcaa cgagctcggc
     2221 accctcatgc ggaccgtggg catgtgaggc cccggactag ggtgtgacca tggcgaaatc
     2281 ggcggacgtc cgcgggccag tgctggtggt gggcacgggg ctgctcggcg catccatcgg
     2341 gctcgccctg cgcgaactgg gcgtcgaggt ggggttgcag gacatctcgc ccgccgccct
     2401 gcacctcgcg cgtgacctcg gcgccgggac ccccgccggc ccggactttc accccgcgct
     2461 cgtggttgtt gccacgcccc cggacgtcat cgtgccggcg gtcgtggcgg cgctgcggga
     2521 ccatcccgac gccgtcgtca ccgacgtcgc ctcggtcaag ggttcactgg cagggcgggt
     2581 cggcgagcag gtgggggcgg cggttgccca gtacgtgggt tcgcatccga tggccggccg
     2641 cgaacgcagc ggccccgcgg ccgcggactc ggacctgttc caggggcgca cctgggtgat
     2701 ctgcccacac g
//
LOCUS       DASRKH010000394         2565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_60340, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000394 DASRKH010000000
VERSION     DASRKH010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60340"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /gene="infC"
                     /locus_tag="VFC06_06355"
     CDS             <1..660
                     /gene="infC"
                     /locus_tag="VFC06_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HZL81539.1"
                     /translation="LMDYGKFKYEAAVKAREARRNQANTEIKEMRFRLKIDEHDYETK
                     KGHVVRFLKGGDKVKVTIMFRGREQSRPEMGRRLLLKLADEVTEFAIVENLPSQEGRN
                     MSLVLGPLKKKAEAKEELRKAREAKKKAENADREPVTVSPKKAAPQAKPVKAKATEAP
                     EADVVEAVKAPEADVVEAPKADAAKAPKAKPVKAKAAKAPEADVVDVAEASEATADSE
                     A"
     gene            751..945
                     /gene="rpmI"
                     /locus_tag="VFC06_06360"
     CDS             751..945
                     /gene="rpmI"
                     /locus_tag="VFC06_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HZL81540.1"
                     /translation="MPKNKTHSGAKKRFKLTGTGKVKHASAMNVHKFEEKTSSRKRRV
                     AIDGILPGADAKKIKKLLGK"
     gene            972..1352
                     /gene="rplT"
                     /locus_tag="VFC06_06365"
     CDS             972..1352
                     /gene="rplT"
                     /locus_tag="VFC06_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HZL81541.1"
                     /translation="MARVKRAVNAQKKRRTTLERASGYRGQRSRLYRKAKEQVTHSLM
                     YSYRDRRARKGEFRKLWIQRINAAARANGMTYNRLIQGLKAAEVEVDRRMLAELAVSD
                     EAAFKQLVDIAKKALPADVNAPVA"
     gene            1366..2208
                     /locus_tag="VFC06_06370"
     CDS             1366..2208
                     /locus_tag="VFC06_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HZL81542.1"
                     /translation="MTERPVGRHLAATLANVRADRVKQVAALAGRSARRRAGQVLVEG
                     PQAVRELVAHRASHVRDVYVLEDALASRGPLALIADAALDAGLFVHPMTPAVRDALSL
                     DCQGILAVADASAVHGDAPTAGARLVAILSQVRDPGNAGAAIRVADAAGADAVVLAGD
                     SVDPTNPKVVRATAGSLFHLPVVRAESLADAVNAAREAGLTILAADVSGDTELGSTQG
                     PDLSGPTAWVFGNEAWGLTADEIALVDHVVRIPIFGQAESLNLGTAAAVCLYASALAA
                     PRSW"
     gene            2219..>2565
                     /locus_tag="VFC06_06375"
     CDS             2219..>2565
                     /locus_tag="VFC06_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81543.1"
                     /translation="MNETPSNRVELSLTGAKPKVEIDGALGIVAKILVDGERAKPVRG
                     GWLIPLSKGGEGRLSVKGFLPGFQKILWQGEEVFVLGSHVGLAERIAMFVPFLLILMA
                     WFLVPVSLALFFM"
BASE COUNT          478 a          816 c          881 g          390 t
ORIGIN      
        1 ctcatggact acggcaagtt caagtacgaa gcggcggtca aggcgcgcga ggcgcgtcgc
       61 aaccaagcca acaccgagat caaagagatg cgtttccgtc tcaagatcga cgagcacgac
      121 tacgagacca aaaagggtca cgtggttcgc ttcctcaagg gcggcgacaa ggtcaaggtc
      181 accatcatgt tccgtggccg cgagcagtcc cgtcccgaga tgggcaggcg tttgctgctg
      241 aagctggccg atgaggtcac ggagttcgcc attgtggaga acctgccgag ccaagaggga
      301 cgcaacatgt cgctcgttct gggtccgctg aagaagaagg cggaagccaa ggaggaattg
      361 cggaaggcgc gtgaggccaa gaagaaggcc gagaacgccg atcgcgagcc ggtcacggtt
      421 tcgcccaaga aggctgcccc tcaggcgaag ccggtgaagg cgaaggcaac ggaggccccc
      481 gaggctgacg tggtcgaagc ggtgaaagcc cccgaggctg acgtagtgga ggcacccaag
      541 gccgacgcgg cgaaggcccc caaggcgaag ccggtgaagg cgaaggcagc gaaggccccc
      601 gaggctgacg tggtcgacgt ggcggaggcc tccgaggcca ccgctgacag cgaggcgtaa
      661 gtctcgtcat tagatgtccg ggcctcgcgc ccggggaatg tgtgccgcgc tcaccagcgc
      721 ggccggacgc tcaagtgaag gagggcgaac gtgcccaaga acaagaccca ttcgggtgcc
      781 aagaagcgat tcaagctgac cggtaccggc aaggtgaagc acgcctcggc catgaacgtg
      841 cacaagtttg aagagaagac gtcgtcgcgc aagcgccgcg tcgctatcga cggcatcctg
      901 cccggtgccg atgccaagaa gatcaagaag ctgctgggca agtagccccc taagacacca
      961 aggagtaccc catggcacgc gtcaagcggg ctgttaacgc ccagaagaag cgccgcacta
     1021 ccctcgagcg cgcatccggt taccgcggac agcgctcgcg cctttaccga aaggcgaagg
     1081 agcaagtcac ccactcgctc atgtactcgt accgggaccg tcgtgcccgc aagggcgagt
     1141 tccgtaagtt gtggatccag cgcatcaatg ccgctgcccg cgcgaacggc atgacctaca
     1201 accgcctcat tcagggcctc aaggccgctg aagttgaggt ggaccgccgg atgctcgccg
     1261 agctcgcagt gagcgacgag gcagccttca agcagttggt cgacatcgcc aagaaggccc
     1321 ttccggccga cgtcaacgct cccgtagcgt aaggcgacac caggcatgac agaacgcccc
     1381 gtcgggcgcc accttgctgc gaccctcgcg aatgtgcgag cggatcgcgt caagcaggtg
     1441 gccgccctgg cggggcgttc tgcacgccga cgcgcgggcc aggtgctggt cgagggcccg
     1501 caagcggtgc gcgaactcgt ggcgcaccgg gcgtcccacg tcagggacgt ctacgtcttg
     1561 gaggacgcgc tggcgtcccg cggcccgctg gcgctcatcg ccgacgccgc gcttgacgcg
     1621 ggcctgttcg tccaccccat gacccctgcg gtacgtgacg cactctcttt ggactgccag
     1681 ggcatcctgg cggtagctga tgcctccgcg gtgcacggcg acgccccgac cgcgggagcg
     1741 cgcctcgtcg cgatcctgtc ccaggtgcgc gatcccggga acgcgggcgc ggcgattcgc
     1801 gttgccgacg ccgccggcgc cgacgccgtg gtgcttgcgg gcgacagcgt cgacccgacc
     1861 aacccgaagg tggtgcgcgc caccgccggc agtctcttcc acttgcccgt ggtgcgcgcc
     1921 gagtcgctcg ccgacgccgt gaacgcggcg cgagaggcgg gcctgacgat tctggccgcc
     1981 gacgtgtcgg gggacaccga gttgggttca acgcagggac cggatctctc tggcccgacc
     2041 gcgtgggtgt ttggaaacga ggcgtggggc ctcaccgccg acgaaatcgc gctcgtggat
     2101 catgtcgtca ggatcccgat cttcggccag gcggaaagcc tgaatctggg caccgcggcc
     2161 gccgtgtgcc tgtatgcgtc cgcattggcc gctccgcgct cctggtagcg tcaacgccgt
     2221 gaacgagact cccagcaatc gcgtcgaact cagcctcacg ggcgcgaagc ccaaggtgga
     2281 gatcgacggc gccctcggca tcgtcgccaa gatcctggtg gacggtgagc gtgccaagcc
     2341 ggtccgcggc gggtggctga ttccgttgag caagggcggc gagggccgac tcagcgtcaa
     2401 gggcttcctg ccgggctttc agaagattct gtggcagggc gaggaggtct tcgtgctcgg
     2461 atcgcacgtg gggctggccg aacgcatcgc catgttcgtg ccgtttttgc tgatcctgat
     2521 ggcgtggttc ctggtgccag tgtcgctcgc gttgttcttc atgag
//
LOCUS       DASRKH010000395         3055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_15890, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000395 DASRKH010000000
VERSION     DASRKH010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15890"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..166)
                     /locus_tag="VFC06_06380"
     CDS             complement(<1..166)
                     /locus_tag="VFC06_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HZL81544.1"
                     /translation="MWAEAARVVALARDAGVSIATAESLTGGLVCAAIVDVPGASAVL
                     KGGIVAYGAEA"
     gene            complement(156..767)
                     /gene="pgsA"
                     /locus_tag="VFC06_06385"
     CDS             complement(156..767)
                     /gene="pgsA"
                     /locus_tag="VFC06_06385"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HZL81545.1"
                     /translation="MLREVPNLLTVLRLIAVPVMLLCLVESGDTQAGWRWLGWGIFVF
                     AAATDALDGFLARRWQAVTAFGKLADPLADKVLVLAALGFLAVHHETPWWPIIIIAVR
                     EVGVTVGRLAVARDVVIPASFGGKAKTALQVAAIFFLLLPTAASWADTLGWWLLLVSV
                     VAAVVTGLDYGWRIFGVARRHGVAPAAPGPVEAGGVDPSGHVG"
     gene            904..2205
                     /locus_tag="VFC06_06390"
     CDS             904..2205
                     /locus_tag="VFC06_06390"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HZL81546.1"
                     /translation="MVLAGVVGAVAMIGASTAAATQITALLIGASRTPPAVTVDAAPP
                     PPPARVAVKEPAIRPVDFTLVAAGDVLPHAPVVNSATTSSGYDFAPLMAGVQPYVEGA
                     DLALCHMEVPVVPPGVAPSGYPLFGSPPEMVRDLAVTGWDGCSTASNHSVDRSFAGIT
                     STLDTMDMYGLGHAGTARTADEAATVQMYDISEGDRTIKVAHIAYAYGLNGLSTPSGM
                     PWSVNTFDADAADAAPIIAAAQAARDQGADVVLASVHCCVEYRTEPTDAQRSLAEKIA
                     ASGLVDLYIGHHAHVPQPIEKLPGGPHGDGMWTAFGLGNFLSNQHPDCCTANSTTGVL
                     LTATFDVDIDDTVTVSVEWTAVTVDRGDHHAMHVLTDVPNGVGSLSQAEVAARHQRVA
                     DAVGSQAPERTTPAESLSDGVVTQSRVGAVPTSAETPASSG"
     gene            complement(2202..2717)
                     /locus_tag="VFC06_06395"
     CDS             complement(2202..2717)
                     /locus_tag="VFC06_06395"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZL81547.1"
                     /translation="MPSAIARHNLSPQRLVVELTEASLVAPNTRIRHELTELRNSGVP
                     ILLDDFGTGVSPLSYLRDLPVSGVKLDMSFVAGIPEDPAGARVSRALGALARELGLAT
                     IAEGIETESQADFLARCGWRYGQGWLFGVAQPAAAVLALDTDFTASLIDPRPQEHDNP
                     FDLPPLHASGA"
BASE COUNT          525 a         1109 c          972 g          449 t
ORIGIN      
        1 tcgcctcagc gccgtacgca acgatgccgc ccttgagcac ggccgacgcc cctggtacgt
       61 caacgatcgc ggcgcacacc agcccgccgg tcaaggactc cgccgtggca atactcaccc
      121 ccgcatcacg cgcgagggcg accacccggg ccgcttcagc ccacatgtcc gctcgggtcg
      181 acgccgcctg cttcgaccgg tccgggcgcc gcgggcgcga cgccatgacg ccttgccacg
      241 ccaaagattc gccacccgta gtccagcccc gtcaccacag cggccacaac ggaaaccaac
      301 aagagccacc acccgagcgt gtccgcccag gacgcggcgg tgggcaggag caggaagaat
      361 atcgcagcaa cttggagtgc cgtcttggcc tttccaccga acgatgcagg gatgaccaca
      421 tctcgcgcga cggccagccg tcccacggtc acgccgactt cccgcaccgc gatgatgatg
      481 atcggccacc acggcgtctc atggtggacc gccaggaagc cgagcgccgc caacaccagc
      541 accttgtccg cgagggggtc ggccagcttt ccaaatgccg tgacggcctg ccagcggcgt
      601 gcgagaaagc catccaaggc atcggtcgcg gcggcaaaca cgaagatgcc ccacccgagc
      661 cagcgccatc ccgcctgggt gtcaccggac tccaccaggc ataagagcat caccggaact
      721 gcgataagcc gcagcacggt aagcagattc gggacttctc gcaacacgac ttcaccctat
      781 cggtgggagc gttgaaacac tcgccgcaca cggattcacc atgcatcggg ccggatgcgc
      841 gacacttgga tcatgttcga gccggttgcc cccgccccgt ccacgcgcac gcgaggccgc
      901 ttcatggtct tagcaggcgt cgtcggagcg gtggcaatga ttggcgcgag caccgccgcc
      961 gcaacgcaga taaccgcact attgatcggg gcatcccgca ctccccccgc cgtcaccgtc
     1021 gacgccgctc ctccgccgcc ccccgcacgg gtggccgtca aggaacccgc aatccgtccg
     1081 gtcgacttca ccctcgtcgc ggcgggcgac gtcctccccc atgcccccgt ggtgaactcg
     1141 gcgaccacgt cgtcgggcta cgacttcgcg cctctcatgg cgggagtgca gccctacgtg
     1201 gagggcgccg acctggccct gtgccacatg gaggttcccg tcgtcccacc cggcgtcgcg
     1261 ccctccgggt acccgctgtt tggttccccg cccgagatgg tgagggacct cgcggtcacc
     1321 ggctgggacg gctgctcgac ggcatccaac cacagcgtcg accggagttt cgcgggcatc
     1381 acgtccacac tcgacacgat ggacatgtac gggcttggtc acgccggcac cgcacgcacc
     1441 gccgacgagg ccgcgacggt ccagatgtac gacatatccg aaggcgaccg gaccatcaag
     1501 gtggcccata tcgcgtacgc gtatggcctc aacggcctgt ccacgccgtc cggcatgccg
     1561 tggtcggtga acacgtttga cgccgatgct gccgacgccg ctcccatcat cgccgcggcc
     1621 caggcggccc gggaccaagg agccgacgtg gtgctcgctt cggtccactg ctgcgtcgag
     1681 taccgcaccg aacccacgga cgcgcaacgc tcactcgccg agaagattgc cgcgtccgga
     1741 ctggtggacc tgtacatcgg gcaccacgcc cacgtccccc aacccatcga gaagttgccg
     1801 ggcggccccc atggcgacgg catgtggacc gccttcggcc tgggcaactt cctgtccaac
     1861 cagcacccag attgctgcac cgcgaacagt acgacgggcg tcttgctcac ggccacgttt
     1921 gacgtcgaca tagacgacac ggtcaccgtc tccgtcgagt ggactgccgt caccgtcgat
     1981 cggggcgacc atcatgcaat gcacgtgctc acggatgtcc ccaacggggt gggatcgctg
     2041 tcgcaagctg aagtggcggc gcgccaccag cgggtcgccg atgccgtcgg gagccaggcc
     2101 ccggaacgca ccacccctgc cgagtcgcta tcagacggcg tggtgacgca gtcacgcgtc
     2161 ggagcggtcc ccacctctgc cgagaccccc gcctcctcgg gctaggcgcc ggaagcgtga
     2221 agagggggca ggtcgaacgg gttgtcgtgt tcttgagggc gcgggtcgat gaggctcgcg
     2281 gtgaagtccg tgtctagcgc gagcaccgct gccgcgggtt gagcgacccc gaagagccag
     2341 ccctggccgt agcgccaacc gcatcgggcc aagaagtccg cctgcgactc ggtctcgatg
     2401 ccctcggcga tcgtcgccag tccgagttct cgcgccaggg cgccgagagc gcgggacacg
     2461 cgcgcacccg cagggtcctc cgggatgccg gcaacgaatg acatgtcgag cttcacgccc
     2521 gagaccggca ggtcgcgcag atagctgagc ggcgagacgc cggtgccgaa gtcgtccagc
     2581 aggatcggca ctcccgagtt gcgcagttct gtgagctcgt ggcggatgcg cgtgttcggg
     2641 gccaccaatg acgcttcggt gagttccacg acgagtcgtt gcgggctgag attgtgacgc
     2701 gcgatcgccg agggcatcga gaccgagtcg caggcagact tcctggcccg atgcggttgg
     2761 cgttacggcc agggctggct cttcggggtc gctcaacccg tggcagcggt gctcgcgcta
     2821 gacacgaact tcaccgcgag cctcatcgac ccgcgccctc aggaacacga caacccgttc
     2881 gacctgcccc ctctgcacgc ttccggcgcc tagcctgagg aggcgggggt ctcggcagac
     2941 gtggggaccg cgccgacccg tgactgcgtc accacgccgt cagatagcga ctcggcaggg
     3001 atcgtgcgtt ccggggcctg gctcccgacg gcatcggcga ctcgctggtg gcgcg
//
LOCUS       DASRKH010000396         2089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_8525, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000396 DASRKH010000000
VERSION     DASRKH010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8525"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VFC06_06400"
     CDS             <1..336
                     /locus_tag="VFC06_06400"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HZL81548.1"
                     /translation="AVLALAACTGSASGDEVTVPVSSTDDGCVVSQTSAPAGNVVFAV
                     TNDGSQETEFYVYKDDGTTIVGEVEHVGPGLTRDLAATLKPGTYITSCDPGMDGDDIR
                     AEFVAADAG"
     gene            424..1218
                     /locus_tag="VFC06_06405"
     CDS             424..1218
                     /locus_tag="VFC06_06405"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013348197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HZL81549.1"
                     /translation="MSAHALAAEAATFLADRTGVPSHDLALILGSGWGGAAELIGDEV
                     ASVEASDVPGFTGHAVAGHHSTFRSLVTAGGKRVLVLGARQHFYQSRDAATVAHAVRM
                     AAAAGCTQLVVTNGCGSTRPHVGPGSVVLISDHLNLTGATPLEGATFVPMGDAYSPRL
                     RDLARTVDGDLIEGVYAQFTGPQYETPAEVKMATIMGADLVGMSTALEVIAAREVGLE
                     VLGLSLVTNLGAGIGTAALNHQEVLDAGAAAAPRISRLLADIVGKM"
     gene            1221..>2089
                     /locus_tag="VFC06_06410"
     CDS             1221..>2089
                     /locus_tag="VFC06_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HZL81550.1"
                     /translation="MDVSEIDDAAVAAAAWLADDPDPSTQAELRGVLEAVASGDTDAV
                     ADLADRMSGLLQFGTAGLRGRLGAGPHRMNRAVVIRAASGLSSYVTAQLGHPGTIVVG
                     YDARHGSTEFAHDTVAVAVAAGHRALLLPSALPTPVLAYAVRALRADAGVMVTASHNP
                     AWDNGYKVYLGASITPEAAGAQIVPPHDAGIAKLIEAVDAVAQVQRATSGWEILSPSI
                     VDDYAAKVAALVPAVDGGAVSQRRAGLKVVLTPIHGVGDATVRKALTAAGFLDLTSVP
                     EQAEPMTCGDRRQL"
BASE COUNT          308 a          756 c          701 g          324 t
ORIGIN      
        1 gcagtcctgg ccctcgccgc gtgcaccggc tcggcttccg gggacgaggt caccgtgccc
       61 gtctcctcga ccgacgacgg ctgcgttgtc tcccagacct ccgcccctgc agggaacgtc
      121 gtcttcgcgg tgacgaacga cggctcccaa gagaccgagt tctacgtcta caaggacgac
      181 ggcaccacga tcgtgggcga ggtggagcac gtcgggccgg gcctcacgcg ggatctcgcg
      241 gccacgctca agcccgggac atacatcacg tcgtgcgacc ccggcatgga cggcgatgac
      301 atccgcgcgg agtttgtcgc ggccgacgcg ggttaggcgc cagcgcggac cgacggacgc
      361 atcgctgcga agcgaaggcc cttcgtctcc aatgaatggg cgagcgggat atacgctcga
      421 tacatgtctg cacacgcact cgcggctgaa gccgccacct tcctcgccga ccgcactggc
      481 gtcccctcac acgacctcgc attgatcctg ggctcgggct ggggcggtgc ggccgaactg
      541 atcggcgacg aggtcgcgtc ggtcgaggcg tccgacgttc cggggttcac gggccacgcc
      601 gtcgctggcc accactcgac gttccggtcg ctcgtgaccg caggcggcaa gagagtgctg
      661 gtgctcggcg cgcgtcagca cttctaccag tcgcgcgacg ccgcgacagt cgcgcacgcg
      721 gtgcgcatgg ctgcggccgc tggctgcacg cagttggtgg tcaccaacgg ctgcggttcg
      781 acacgccccc acgtcggccc cggatccgtc gtcctcattt ccgatcacct caacctcacc
      841 ggcgccacgc cgctcgaggg agcgaccttc gtgccgatgg gcgatgccta ctccccgcgc
      901 ctgcgcgacc tggcccgcac agtggacggc gacctgatcg agggcgtcta cgcgcaattt
      961 accggcccgc agtacgagac cccggccgag gtgaagatgg cgaccatcat gggcgccgac
     1021 ttggtgggca tgtccaccgc acttgaggtc atcgccgcgc gcgaggtggg cctcgaggtg
     1081 ctgggtctgt ccctggtcac caacctcggc gccggcatcg gcactgcggc cctcaaccac
     1141 caagaggtgc tggacgccgg ggccgccgcg gccccgcgca tctcgcgcct gctggccgac
     1201 atcgtgggga agatgtagtc atggacgttt ccgagatcga cgacgccgcc gtcgcagccg
     1261 ccgcttggct tgcggacgat cctgatccct ccacccaggc ggagctccga ggagtgctcg
     1321 aggcggtggc gtcgggagac accgatgccg tggccgacct ggccgatcgc atgtcgggcc
     1381 tcctgcagtt cggcaccgcc ggcttgcggg gacgccttgg cgccgggccc caccgcatga
     1441 accgcgccgt ggtcatccgc gcggcttcgg ggctctcctc ctacgtgacc gcccaactgg
     1501 gtcacccggg cacgatcgtg gtcgggtacg acgcacgcca cggctcgacc gaattcgccc
     1561 acgacaccgt cgcggtggcc gtagccgccg ggcatcgtgc cctgttgttg ccctccgccc
     1621 tgcccacgcc cgtgctcgcg tacgcggtgc gcgcacttcg cgccgacgcg ggagtgatgg
     1681 tgacggcttc ccacaacccc gcatgggaca acggctacaa ggtgtacctg ggtgcgtcga
     1741 tcacgccgga ggcggcgggt gcgcagatcg ttcctcctca cgacgccggt attgcaaagc
     1801 tcatcgaagc cgtcgacgcc gtggcacaag tgcagcgcgc cacatcgggc tgggagatcc
     1861 tctcccccag catcgtcgac gactatgcgg ccaaggtggc cgcactcgtg cctgcggtgg
     1921 atggcggcgc ggtgagccaa cgccgtgccg gtctcaaggt ggtgctcacc cccatccacg
     1981 gcgtcggcga cgcgaccgtc cggaaggcgc taacggccgc gggcttcctc gacctgacgt
     2041 cggtgcccga gcaggccgag cccatgacgt gtggcgatcg ccgccaact
//
LOCUS       DASRKH010000397         5199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_9197, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000397 DASRKH010000000
VERSION     DASRKH010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9197"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VFC06_06415"
     CDS             complement(<1..422)
                     /locus_tag="VFC06_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZL81551.1"
                     /translation="MERRSVANTDVGATKPEYGASDITVLEGLEAVRKRPGMYIGSTG
                     ERGLHHLVYEVVDNSVDEALAGHCDTITITLMPDGGVRTTDNGRGIPVDMHPIEGKPT
                     LEVVMTILHAGGKFGGSGYAVSGGLHGVGISVVNALSR"
     gene            complement(638..1324)
                     /locus_tag="VFC06_06420"
     CDS             complement(638..1324)
                     /locus_tag="VFC06_06420"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HZL81552.1"
                     /translation="MTTGPSQGEGRVSRQQGGDPLNEGATYREDESGLLNEGATYREN
                     DDTAAEKPSRPAPSKDAAAQAREAMERARASARERGAARISPQTARRRKDAKREASGG
                     GSPMPFGTGRDPRAMSDNVTALLRRMGWTENIEVAGVTARWREVIGDQIADHCEPVSF
                     EAGVLLLRASSSAWATQMQLMSGQVRHRLNEEFGREVVKELRFIGPSDRHWVKGPRRV
                     KGRGPRDTYG"
     gene            complement(1321..2586)
                     /gene="recF"
                     /locus_tag="VFC06_06425"
     CDS             complement(1321..2586)
                     /gene="recF"
                     /locus_tag="VFC06_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012630743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HZL81553.1"
                     /translation="MRITHLILTDFRSYEAVQVDFGPGVTTLVGRNGHGKTNVVEAVR
                     YLSTLSSHRVATDAPMLRLGAQRAIVSGRVQRGDRSLTLEVTMVPGKANQARLNRGNA
                     KPRDLVGILRTVVFAPEDLTLVKGDPGTRRAFMDELCVALQPPIAGDLADYDRVLRQR
                     SSLLKSARGKPRFDVSTLDVWDEKLADLGARIMHARLAAMEALAPHVDQAYADVAPGE
                     GKCTITYATAVEVPLDDDTADSADTAGKGGVQLTATPTATPTATPTATPTAAPTAATP
                     VADIGAALLGRMVERRSQEIERGVCLVGPHRDDLDIRIGELPAKGYASHGESWSCALA
                     LRLGSFALLADDGDDGEPVLILDDVFAELDSGRRAALAGRVVGAGQVLITAAVSEDVP
                     EQLRDHPEGHRILRVTPGMVTDASGEVAE"
     gene            complement(2593..3720)
                     /gene="dnaN"
                     /locus_tag="VFC06_06430"
     CDS             complement(2593..3720)
                     /gene="dnaN"
                     /locus_tag="VFC06_06430"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HZL81554.1"
                     /translation="MKFSVERDVLNDAVAWTARAVPHRPPVPVLAGVLIEADSAGTVK
                     LSSFDYEVSARGEFAADVEDSGAVLVSGRLLREISNALPHKLVKFELEGSKVSVTCGA
                     SRFSLATMPVDQYPALPNMPDSSGTVDGGELLSAAQQVQSAASRDETLPILTGVRMEI
                     EGERISLLATDRYRLAFRELTWKPGTPDISAIALVRTKTLTDTAKALGSGEVQLALSS
                     GQGVDLIGFSAGGLVTTSLLMDGEYPQVRRLFPETSAITAVVSTAELIEATRRVALVA
                     ERNAAVRLAFSEGEVVLDAGHSDDAQASEAIEATVEGGEITVAFNPGFLLDGLSALGA
                     PYVRLGFTQDTKPVDFVGLDKPGGTIASEFRYLLVPIRIAG"
     gene            complement(4102..>5199)
                     /gene="dnaA"
                     /locus_tag="VFC06_06435"
     CDS             complement(4102..>5199)
                     /gene="dnaA"
                     /locus_tag="VFC06_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019482050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HZL81555.1"
                     /translation="DEPAQESRERRPATRVAAPAVEDPHLNPRYTFDTFVPGSSNRFA
                     HAAALAVAESPGKTYNPLFIYGDSGLGKTHLLHAIGHYTRQIKPQTRVRYVNSEEFTN
                     DFINSIRDDRSLSFKRTYREVDVLLVDDIQFLQGKEQTLEEFFHTFNALHNAGKHVVI
                     TSDVSPRHLNGIEERMRTRFEWGLMTDVQPPDLETRIAILRKKAQADDVQAPPDVLEY
                     IASNITSNIRELEGALIRVTAFAALNKQQVDLSLAQVVLKDLISDDDSQITASSIIGK
                     TAEYFGITMEELTGTSRSRVFVTARQIAMYLCRQLTDLSLPKIGEHFGGKDHTTVMYA
                     VKKVEDQIAQRRQMYNQVNEIHARIKQQSRG"
BASE COUNT          872 a         1648 c         1733 g          946 t
ORIGIN      
        1 ctgcgcgaca acgcgttcac cacggagatg ccgacgccgt gcagtccgcc cgacacggcg
       61 tagccgctgc cgccgaactt tccgcccgcg tggaggatcg tcatgacgac ctcgagcgtg
      121 ggcttgccct cgatcgggtg catgtcgacc ggaatgccgc ggccgttgtc ggtggtgcgc
      181 acaccgccgt ctggcatcag cgtgatggtg atggtgtcgc aatgccccgc cagcgcctca
      241 tcgacggagt tgtccacgac ctcgtagacc aagtggtgca gaccgcgctc cccggtggag
      301 ccgatgtaca tgccggggcg cttgcgcacg gcctcgagac cctcgagaac cgtgatgtcg
      361 cttgcgccgt attcgggctt ggtcgcccct acgtcggtgt tggccacaga tcgcctttcc
      421 acgcccctca cgcgacccga ttcgggtcgc gtcctacctc ggtccattct acgcggtctg
      481 agcggctctc ccgggccatt cagggggatt ctggggaaag ctgaatgatg cgggtctcag
      541 ttggcggcat ttcgcttggt cctcagtggc gttggaaggg gtcgatccgg gaccttttgg
      601 ggatgcggcc ctcggcgctg gttcctgcgc gatcccgcta gccgtacgtg tcgcgagggc
      661 cccggccctt gacgcgtcgc gggcccttga cccagtgccg gtcgctcggc ccgatgaacc
      721 gcagctcctt cacgacctcg cggccgaact cctcattcaa ccggtgccgg acctggccgg
      781 acatgagctg catctgggtc gcccacgcgc ttgacgaggc ccgcaacaag aggacgccgg
      841 cctcgaagct caccggctcg cagtgatccg cgatctggtc gccaatgacc tcgcgccagc
      901 gcgcggtgac cccggcgacc tcgatgttct ctgtccagcc catgcggcgc agcagcgccg
      961 tgacgttatc gctcatcgcg cgcgggtcgc ggccagtgcc gaatggcatc ggtgagccgc
     1021 ctcccgaggc ttccctcttg gcgtccttgc ggcggcgagc ggtctggggg gagatgcggg
     1081 ccgctccgcg ttcgcgcgcc gaggctcgcg cccgctccat cgcctcgcgg gcctgcgccg
     1141 ccgcgtcctt ggagggcgcg ggccgggagg gcttctcggc cgcagtgtca tcattctcgc
     1201 ggtacgttgc gccttcgttg agcagacccg actcgtcctc gcggtacgtt gcgccttcgt
     1261 tgagggggtc cccaccctgc tggcggctca cccggccctc accctgactc ggccccgtcg
     1321 tcactcggcc acctccccgg acgcgtcggt caccatgccg ggcgtcacac gcaggatccg
     1381 gtggccctcg ggatgatcgc gcaactgctc gggaacgtcc tcgctcacgg ccgccgtgat
     1441 gagcacctga cccgcgccga cgacgcggcc cgcgagggcc gcgcggcggc cggaatccaa
     1501 ttctgcgaag acgtcatcca ggatcagcac gggttcgccg tcgtcgccat cgtccgcgag
     1561 cagtgcgaat gatcccagtc gcagcgcaag cgcacaggac cagctttccc cgtgactcgc
     1621 gtagcccttg gcgggtaatt cgccgatgcg gatgtcgagg tcgtcgcggt ggggccccac
     1681 caagcacacg ccgcgctcga tttcttggga tcggcgctcg accattctgc cgagcagcgc
     1741 cgccccgata tccgcgaccg gggtcgcggc cgtcggcgct gcggtcggtg ttgcggtcgg
     1801 tgttgcggtc ggtgttgcgg tcggtgttgc ggtcagctgc actcccccct tgcccgcggt
     1861 gtccgcagag tccgctgtgt cgtcgtcgag cggcacctca accgccgtcg catacgtgat
     1921 ggtgcacttg ccctcgcccg gcgcgacatc cgcgtaggcc tgatcgacgt gcggcgccag
     1981 agcctccatc gccgcaaggc gcgcgtgcat gatgcgagcg cccagatccg cgagcttctc
     2041 gtcccacacg tcgagcgtag agacgtcgaa tcgcggtttg ccgcgcgcgg atttcagcaa
     2101 cgatgagcgc tgacgcagta cgcggtcata gtcggcaaga tccccggcaa tcggcggctg
     2161 caaagcgaca catagctcgt ccatgaacgc acgccgggtg ccgggatcac ccttgacgag
     2221 cgtgaggtcc tcgggcgcga agacgacggt gcggagaatc ccgacgaggt cgcgcggctt
     2281 ggcgttgcca cgattgagtc tcgcttggtt ggccttgccc ggaaccatgg tgacctcgag
     2341 tgtgagcgac cgatctcccc gctgaacccg gcccgagacg atcgcacgct gagcgcccaa
     2401 cctcagcatc ggcgcatctg tggcgacccg gtgggaggac agcgtggaca ggtagcgaac
     2461 cgcctcgacc acattggtct tgccgtgacc gttgcgtccg acgagcgtgg tgacccccgg
     2521 accgaagtcg acctggacgg cctcgtagga gcggaagtcg gtgaggatca ggtgcgtgat
     2581 gcgcatcgac cgctagccag cgatgcggat gggcaccaag aggtagcgga actccgaggc
     2641 aatggtgccg cccggtttgt cgagtccgac aaagtccact ggcttggtgt cctgcgtgaa
     2701 gccgagtcgc acatacggcg cgcccagagc gctcaacccg tcgaggagga atcccgggtt
     2761 gaaggcgacg gtgatctctc caccctcgac cgtggcttcg atggcctccg aagcctgggc
     2821 gtcgtcgctg tggccggcgt cgagaacgac ctcgccctcg gagaatgcga gtcgcactgc
     2881 ggcattgcgt tcggccacca gggcgacgcg tcgcgtcgcc tcaatgagct cggcggtaga
     2941 caccacggcg gtgatggccg acgtctcagg aaacaggcga cgcacttgcg ggtactcgcc
     3001 atccatcagg agcgaggtgg tcaccagtcc gccggcactg aaaccgatga ggtcgacacc
     3061 ctgcccagag ctcagcgcaa gctgaacctc cccagaacca agtgccttgg ccgtgtcggt
     3121 cagcgtcttc gtccgaacca aggcgatcgc cgagatgtcg ggggtgccgg gcttccacgt
     3181 gagttcgcga aacgcaagcc ggtagcggtc ggtggcgagc agcgaaatgc gctcaccctc
     3241 gatctccatc cggacgccag tgaggatggg caacgtctcg tctcgagacg ccgcggactg
     3301 cacttgctgc gcagcggaca acagttcgcc gccgtccacg gtgccggaac tgtccggcat
     3361 gttgggaagt gccgggtatt ggtccaccgg catcgtcgcg agcgagaacc gggaggctcc
     3421 gcacgtgacg gaaaccttgg atccctcgag ctcgaacttc acgagcttgt gcgggagcgc
     3481 attggagatc tcacgcaaga gccgcccgga aaccagaact gcaccgctgt cctcaacgtc
     3541 ggccgcgaat tcaccgcggg ccgagacctc gtagtcgaaa ctcgaaagct tgaccgtgcc
     3601 ggcgctatcg gcctcaatga ggactcccgc cagcacgggc acagggggac gatgcggaac
     3661 ggcacgcgcg gtccaagcaa ccgcgtcgtt cagcacatca cgctctacag aaaacttcat
     3721 cgatctcccc tttcgggtca cagactacgc cacgggaccg acgcgtctag tcccgaaacg
     3781 agaagccttc ggtgggccca atgaaggccc ttgtgtagaa gtaatagaga aaggttgtag
     3841 tggtcatagg ggttcggaaa tctgtggaga aactacaagt ctcctgctag gagcggcgag
     3901 ccgccagtgg acgagacatg gaccctgcgc ggtggggctg aggagagcct gtgagcgacc
     3961 agtcgtcgta cacaggcagt agtgattcat tcacaggttg tcccatgtgg gtgaaggggc
     4021 gcccaaaagt tatgcacaga gttatccaca tgtgtgagtc atacttctgg gggcatacaa
     4081 aacggacaaa ccgcgcaaag gttatccgcg cgactgttgc ttgatgcgcg cgtggatctc
     4141 gttgacctgg ttgtacatct ggcgacgttg cgcgatttgg tcttcgacct tcttcacggc
     4201 gtacatgacg gtggtgtggt ccttgccgcc aaagtgttcg ccgatcttgg gcagagatag
     4261 gtccgtgagc tggcgacaca ggtacatagc gatctgcctg gccgtgacaa acaccctcga
     4321 cctggaggtg ccggtcagtt cctccatggt gatgccgaag tactccgcag tcttgccgat
     4381 gatgctggac gctgtgatct gagagtcgtc gtcgctgatg aggtccttca acaccacctg
     4441 tgcgagcgag aggtcgacct gctgcttgtt cagggcagcg aaggcggtga cgcggatgag
     4501 cgcaccctca agttcgcgga tgttgctggt gatgtttgac gcgatgtact caagaacgtc
     4561 gggcggcgcc tgaacatcgt ccgcttgcgc cttcttgcgg aggatcgcga tacgggtctc
     4621 aaggtcgggc ggctggacgt ccgtcatcag gccccactcg aagcgcgtcc gcatgcgctc
     4681 ctcgatgccg ttgaggtggc gcggtgagac atcggaagtg atgacgacgt gcttgccggc
     4741 gttgtggagg gcgttaaacg tgtggaagaa ctcttcgagc gtctgctctt tgccttgcag
     4801 gaactggatg tcgtccacca gcagcacgtc cacctcgcgg taggtgcgct tgaagctcag
     4861 cgagcgatcg tcgcgaatcg agttgatgaa gtcgttggtg aactcttccg agttgacgta
     4921 gcggacccgg gtctgcggct tgatctggcg cgtgtagtga ccgatggcgt gcaggaggtg
     4981 agtcttgccc agcccagagt cgccatagat gaagagggga ttgtacgtct tgccggggct
     5041 ctcggcgacc gcaagggcag cagcgtgagc gaagcggttg gacgaaccag gcacgaaggt
     5101 gtcgaacgtg tagcgggggt tgaggtgtgg atcctcgacg gcgggcgcag cgactcgggt
     5161 ggcggggcga cgctcacgcg attcctgcgc aggctcatc
//
LOCUS       DASRKH010000398         2128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_52512, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000398 DASRKH010000000
VERSION     DASRKH010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52512"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VFC06_06440"
     CDS             complement(<1..495)
                     /locus_tag="VFC06_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HZL81556.1"
                     /translation="MILPFTQSERSTVGIEWELQLIDSDSLDLRQCAAAVLAAVSPPG
                     GEKHPNIHPELLLNTVEVVSSPSHTVPEAVADLERSIAELAPIMSRLRVELATAGTHP
                     FANPLYQRVTNKERYARLVDRTRYWGQQMLLFGMHVHVGIEDQRKVIPILNALMTRHA
                     HLQAL"
     gene            complement(492..1706)
                     /locus_tag="VFC06_06445"
     CDS             complement(492..1706)
                     /locus_tag="VFC06_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HZL81557.1"
                     /translation="MGTESLRRLLEPEGWALLASLPPYEEAEAVRLNESLRAAGHDAD
                     LVATVLTQQHLRARARAKLGEFAANMLFTEKGLQQATRLVVAAHHANRYRQAGCTRVA
                     DLGCGLGADSIALAALGIAVDGVEQDETTAAFATINLMPFPNARVVNGDALSHDLSGY
                     DGVFADPARRTERGRVFDPEDYSPPLPRLFELRTVVPALGVKVAPGIGYGHLPADTHA
                     QWVSVGGDVVEAGLWFGPLARTPGRSALVIDAAGRSAALDCAARPDEPAERAPVGPLQ
                     RYLYEPDGAVIRAGGVATLARRLGATTVSESIAYLTGPSAVTSPFAEAFEVLDHLPYS
                     VKRLSSYLAARGIGRLEIKKRGVDVVPDRLRAQLGLRGDSGATVVLTRLEGRHSAVIV
                     RRMRPGAGTMDS"
     gene            1929..>2128
                     /locus_tag="VFC06_06450"
     CDS             1929..>2128
                     /locus_tag="VFC06_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HZL81558.1"
                     /translation="MSVSIKPLEDRIVVQTLDAETTTASGLVIPDTAKEKPQEGEVMA
                     IGPGRIDDNGNRVPLDVAVGDKV"
BASE COUNT          314 a          719 c          794 g          301 t
ORIGIN      
        1 cagggcctgg aggtgcgcat gccgcgtcat tagggcgttg aggatcggga tcaccttgcg
       61 ctggtcctcg atgcccacgt ggacgtgcat cccgaagagc agcatctgct ggccccagta
      121 ccgggtccgg tccacgagcc gcgcgtagcg ctccttgttg gtgacccgct ggtagagggg
      181 gttcgcgaac gggtgcgtcc ccgcggtggc gagctcgacc cgcaaccggg acatgatcgg
      241 ggcgagctcc gcgatcgagc gctcgaggtc cgccacggcc tccggaaccg tgtgcgacgg
      301 cgaggagacg acctcaaccg tgttgagcag cagctccggg tggatgttgg ggtgcttctc
      361 tccccccggg ggcgaaacgg ccgcgaggac ggctgcggcg cactgccgca ggtccaagga
      421 gtccgagtcg atgagttgca gctcccactc gatgccgact gtggagcgct cggactgcgt
      481 gaagggcagg atcatgagtc catggtgcct gctccggggc gcatgcgtcg aacgatcacc
      541 gcgctgtgcc gcccctccag ccgtgtcagg acgacggttg ccccggagtc cccgcggagc
      601 cccagctggg ctcggaggcg gtccggcacc acgtcgacgc cgcgcttctt gatctcgagg
      661 cggccgatgc cgcgggccgc gaggtagctg gagaggcgct tgacggagta gggcaggtgg
      721 tcgaggactt cgaaggcctc cgcgaacggg ctcgtcaccg cactcgggcc ggtgaggtag
      781 gcgatcgact cactgaccgt cgtggccccg agccgccgcg caagcgtggc gacgccgccc
      841 gcacggatca cggcgccgtc gggctcgtac aggtagcgct gcaacggccc cacgggggcc
      901 cgctcagccg gctcgtccgg ccgggccgcg cagtcgaggg ccgcggaacg acccgcggcg
      961 tcgatgacga gcgcgctgcg tccgggcgtc cgtgcgaggg ggccgaacca caggcccgcc
     1021 tcgacgacgt cgccccccac gctgacccac tgggcgtggg tgtcggcggg gaggtggccg
     1081 tacccgatgc cgggagccac cttgacgccc agcgcgggca cgaccgtgcg caactcgaag
     1141 aggcggggga gcgggggcga gtagtcctcc gggtcgaaca cgcggccgcg ctcggtccgg
     1201 cgagccgggt ccgcgaacac accgtcgtag ccgctgaggt cgtggctgag ggcgtcgccg
     1261 ttgaccaccc tggcattggg gaacggcatc aggttgatgg tggcgaacgc ggcggtggtc
     1321 tcgtcctgtt ccacgccgtc gacggcgatc cccagcgccg ccagggcgat cgagtcggcc
     1381 ccgagcccac agccgaggtc ggcgacacgg gtgcagccgg cctggcggta gcggttggcg
     1441 tggtgggccg cgacgacgag acgggtggcc tgctgcagcc ccttctcggt gaagagcatg
     1501 ttcgcggcga actccccgag cttcgcgcgg gcccgtgcgc gcaggtgctg ctgcgtgagg
     1561 accgtggcga ccaggtccgc gtcgtgaccg gccgcccgga ggctttcgtt caggcgcacg
     1621 gcctccgcct cctcgtaggg agggagcgat gcgaggagcg cccagccctc cggctcaagg
     1681 aggcgtcgga gcgattcggt gcccatcgcg ccattgtcgc accccgtacc gggcaggcgg
     1741 tcccggccgc cggtgcaccg ccgtcggcgg cccgactcgc tggcactcgc cttgaccgag
     1801 tgctaacggg ggcatagatt ggtcgtgggc gcgggcatcc gcgtgagtga ctgggcttcg
     1861 acccccgcga cggcggagca caccactcga cagttgaaca ttcgtcgtca caggaaggga
     1921 gtgccgcagt gtcggtctcc atcaaaccac tcgaggaccg catcgtcgtg cagaccctcg
     1981 atgccgaaac caccacagcg tccgggctcg tcatcccgga caccgccaag gagaagcccc
     2041 aggagggcga ggtcatggcg atcggtccgg gtcgcatcga cgacaacggc aatcgcgtcc
     2101 cgctggacgt ggccgtgggc gacaaggt
//
LOCUS       DASRKH010000399         2941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_64980, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000399 DASRKH010000000
VERSION     DASRKH010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64980"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VFC06_06455"
     CDS             <1..258
                     /locus_tag="VFC06_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HZL81559.1"
                     /translation="VKLFPAALGGPAYLRALRDPLPDVPFVPVGGVDIALAADYLAAG
                     AVAVGVGSPLVGDAVRGGDLAALEARATAFLAVTRASHGSR"
     gene            255..1199
                     /locus_tag="VFC06_06460"
     CDS             255..1199
                     /locus_tag="VFC06_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018775434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HZL81560.1"
                     /translation="MTGPDVVTFGETMVSLRTGTVLRLGGTLSMTMAGAESNVAIGLA
                     RLGHTVRWCGRVGADEVGAFILRTLRAESVAVDTVVVDASRPTGLMLAERRVADVSRV
                     SYYRAGSAGSALSATEGAASVAEAPRILHVTGITPALSPSAADAVAVAVRAARGVGTL
                     VSVDINYRSKLWTPEQARPVLSELVRSADIVIASEDELGLVAPAGEAAAAAELGEAGV
                     SHLVITRGARGATVWQDGAAHDAPAVPVAVRDTIGAGDAFTAGYLSGVLDGLSPAGAL
                     HRGTVTAAFAVAAVGDWEGLPTRSELALLDSEAGSTLR"
     gene            complement(1307..2545)
                     /gene="manD"
                     /locus_tag="VFC06_06465"
     CDS             complement(1307..2545)
                     /gene="manD"
                     /locus_tag="VFC06_06465"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011690372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HZL81561.1"
                     /translation="MNIDKAEVIVTSPDRNFVTLRLTTDDGLVGIGDATLNGRELAVV
                     AYLKEHVAPLLIGKDAGRIEDMWQFLYRGAYWRRGPVTMAAIAAVDTALWDIKGKAAG
                     MPVYQLLGGASRDGLMAYGHASGKTLPELFDSVREHQELGFKSIRIQTGVPGLKAIYG
                     IASNTAGTTHEGVRYDHEPAQRGALPMQEDWDTRAYLRHIPGVFEAVRNEFGPELPLL
                     HDGHHRMTPIQAAKLGKSLEPYDLFWLEDCTPAENQEALRLVRQHTTTPLAIGEVFNT
                     VWDYQTIIREQLIDYVRSAVTHTGGISHLKKLLEFAAQYQIKSGMHGPTDVSPVGMAA
                     AMHLGLSIHNFGIQEYMQHGEKTNQVFRQTFTWSDGLLHPGDEPGLGVTLDVDEAGKY
                     PYEQAYLPFNRLADGTVHDW"
BASE COUNT          435 a          958 c          985 g          563 t
ORIGIN      
        1 gtcaagctgt tcccggccgc cctcggcggc cccgcctacc tgcgcgcgtt gcgcgaccca
       61 ctgcccgacg tgccattcgt cccggtgggc ggggtcgaca tcgcactcgc cgccgactac
      121 ctcgcggctg gcgccgtcgc ggtcggagtg ggcagtcccc tggtcgggga tgccgttcgt
      181 ggcggtgacc tggccgcact ggaggcacgc gctacggcgt tcctcgccgt gactcgggcc
      241 tcgcacgggt cgcgatgact gggcccgacg tcgtcacctt cggcgagacg atggtcagcc
      301 tccgcacggg cactgtcctg cggcttggcg ggacgctgtc gatgaccatg gccggcgccg
      361 agtccaacgt cgcgattggg cttgcgcgcc tgggtcacac ggtgcgctgg tgcggacggg
      421 tgggtgccga cgaggtcggc gccttcatcc tgcggaccct gcgggccgag tcggtggcag
      481 tggacacggt cgttgtcgac gcgagccgac ccaccggact tatgctggct gagcgtcgcg
      541 ttgccgacgt ctcacgggtg agttactacc gcgcgggttc ggccggttcc gcactctcag
      601 cgacggaagg cgcggccagc gtcgccgagg cacctcgaat cctgcacgtg acgggaatta
      661 ccccggcact gtcgccgtcg gcggcggatg ccgttgccgt ggcggtccgc gccgcccgag
      721 gcgtcgggac actcgtcagt gtggacatca attaccggtc gaagttgtgg acgcccgaac
      781 aggctcgtcc agtgctctcc gagctggtcc gcagcgcgga catcgtgatc gcctcggaag
      841 acgagctggg gcttgtcgca cccgcggggg aagccgctgc ggcggccgag ctgggggagg
      901 ccggcgtcag ccacctcgtc atcacgcgag gtgctcgcgg cgcaaccgtc tggcaggacg
      961 gcgcagcgca cgatgcgccg gccgtccccg tcgcggtgcg ggacacgatc ggcgccggcg
     1021 atgccttcac cgccgggtac ctgtccgggg tgcttgacgg gctttctcct gcgggtgcgc
     1081 tccatcgcgg caccgtcacc gcagcgttcg ccgtcgcggc ggtgggtgac tgggaggggt
     1141 tgccgacccg cagcgagctc gctctcctgg attccgaggc cggttcaaca ctgcgctgac
     1201 cagactgtgc tgaccagcca cggatcgatt cgtgggatgc cggccaagta ccgtcggccc
     1261 atctacccac gatccgttga tcgcgctgca gtcaatcgcg ctgcggtcac cagtcgtgga
     1321 ccgtgccgtc ggcgagcctg ttgaatggca ggtacgcctg ctcgtagggg tacttgcccg
     1381 cctcgtccac atcgagcgtg acgcccaggc ccggctcgtc ccctgggtgc agaaggccgt
     1441 cgctccacgt gaaggtctgg cggaagacct ggttggtctt ctcgccgtgc tgcatgtact
     1501 cctggatgcc gaagttgtga atcgacaggc ccaggtgcat ggcggcagcc atgcccacgg
     1561 gtgacacgtc ggtggggccg tgcatgccgg acttgatctg gtattgcgcc gcgaactcga
     1621 gcagcttctt gaggtgcgaa ataccgcccg tatgagttac ggccgagcgc acgtagtcga
     1681 tgagctgctc gcgaatgatg gtttggtagt cccagacggt gttgaacacc tcgccgatcg
     1741 ccagtggcgt ggtggtgtgt tggcggacca gccgcagcgc ttcctggttc tctgcgggcg
     1801 tgcagtcctc aagccagaac aggtcatacg gttcgagaga cttgcccaac ttcgcggcct
     1861 gaatgggcgt catgcggtgg tggccgtcgt gaagcaaggg cagttctggc ccgaactcgt
     1921 tgcgtacggc ctcgaaaacc cctggaatgt ggcgcaagta cgcacgcgta tcccagtcct
     1981 cctgcatcgg cagggcgcct cgctgggccg gctcatggtc gtagcgcacg ccttcgtgcg
     2041 tggtgccggc ggtattcgag gcgatgccat agattgcctt gagccccgga actcccgtct
     2101 gaatgcggat cgacttgaaa ccgagctcct ggtgctcccg gacgctgtcg aacagttccg
     2161 gtagcgtctt gccggacgcg tgcccataag ccatgagacc gtcgcggctc gccccgccga
     2221 gcagttggta gaccggcatg cccgcggcct tgcccttgat gtcccacagt gccgtatcga
     2281 cggccgcgat ggccgccatc gtcaccggac ccctgcgcca atacgcaccg cggtacagga
     2341 actgccacat gtcctcgatg cgaccggcgt ccttgccgat cagcaacggc gccacgtgct
     2401 ccttgaggta ggcgaccacg gcgagttctc gcccgttgag ggtcgcgtcg ccaatcccga
     2461 cgaggccgtc atctgtggtg agtctcagtg tgacgaagtt gcggtccggg ctggtgacga
     2521 tgacttcggc cttgtcgatg ttcatggcgt tccttcgttg attcgtggtg tcgtcagttg
     2581 actagcgacg gcctgcggtt gcccgacgtg gtcccgcgct tgcccttgca gttgacgacg
     2641 cccttgcctt agcctcccac gcgctggtcc cgatctcctt gatgagggag cgacgaacga
     2701 ttcgcctccc agtgcgtcat ccaacttgta cttcgctgtg aaagttcatc ccctccctcg
     2761 ggtcactgct cgctcagttg tagaaacaca cctcgacgct atggccccga aggcaggcgc
     2821 caccctaaac tggctactta tacgggataa gtcatcccaa tcatatgatg aaagcaaccc
     2881 cggagagtaa gtcatgatat ttagcgtggt ccggtgcgct ttggagggat cggcgccatg
     2941 a
//
LOCUS       DASRKH010000400         4101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_49536, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000400 DASRKH010000000
VERSION     DASRKH010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49536"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VFC06_06470"
     CDS             complement(<1..300)
                     /locus_tag="VFC06_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81562.1"
                     /translation="MSGIWDWLVHLSGNLEIGILDLVESLWIYPSVFAVSLIDAVIPV
                     VPSESVIIGAASAWQSVGKPILPLLFLAGAAGAWCGDQTAYFVGTKVDVRHVKLFK"
     gene            complement(297..1658)
                     /gene="glmM"
                     /locus_tag="VFC06_06475"
     CDS             complement(297..1658)
                     /gene="glmM"
                     /locus_tag="VFC06_06475"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HZL81563.1"
                     /translation="MARLFGTDGVRGVANRDITAELALDLAVAAAHVLAERGEFSGHR
                     PRAVLGRDTRLSGQFLSSAVAAGLASAGVDVIDLGVFPTPGIAYLTGSVEADLGVVIS
                     ASHNPMPDNGIKFFARGGVKLPDAVEEAIEARVGEPWERPTGGDVGEVIASDELAADY
                     LAHLVHAVQEFGGAESPLKGLTIVVDGAHGAASVVGPAALEAAGATVVRLHCAPDGRN
                     INAGVGSTHLEPLAAAVVNEGAHMGVAFDGDADRCLAVDEAGAIVNGDHILGLLAIAM
                     RDAGKLYRDTMVATVMSNLGLHHAMREAEIDIVETAVGDRYVLEAMREGGYTLGGEQS
                     GHVIVSRYATTGDGVLTSLLLGSLVAKSGSLAELARGVQTLPQVLINVTGVDRSRVHT
                     DAELGKELESVRTELGDSGRVLLRASGTEPLIRVMVEAATPEAAQQHADRLAAVVAVR
                     LSL"
     gene            complement(1674..2114)
                     /gene="rpsI"
                     /locus_tag="VFC06_06480"
                     /pseudo
     CDS             complement(1674..2114)
                     /gene="rpsI"
                     /locus_tag="VFC06_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016460311.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
     gene            complement(2363..2806)
                     /gene="rplM"
                     /locus_tag="VFC06_06485"
     CDS             complement(2363..2806)
                     /gene="rplM"
                     /locus_tag="VFC06_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016473395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HZL81564.1"
                     /translation="MRTFSPKPGDLTSKWHIIDANDVVLGRLASQAAALLRGKHKATF
                     APHVDSGDFVIVINAEKVALTGAKLTDKMVYRHSGFPGGLKTKPIGELIADQPTKVIE
                     NAVRGMLPRTKLGRAQLSKLKVYAGPEHPHAAQQPAPFEISQVAQ"
     gene            complement(2969..3928)
                     /locus_tag="VFC06_06490"
     CDS             complement(2969..3928)
                     /locus_tag="VFC06_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HZL81565.1"
                     /translation="MTELHSGEVVRARLDLSYDGGAFSGWGTQPGLRTVQGELEAGLV
                     RVVRGPAGRSYDEPTLRITVAGRTDGGVHARGQVAHVDIPAVAWRRLPGKTSRTPAHA
                     LRERLGGVVGADIVIRAASEAPEGFDARFSALERHYEYRICDRRALRDPLRRGHVAWI
                     DRSLDVDALNTASATLLGLRDFTAFCRGREGATTIRELKEFSWQRVESGADAGLLVAT
                     VRADAFCHAMVRSLVGAVMAVGYGRRDLVWLEMVSTHPVRSPAIKVAAPRGLTLEEVV
                     YPGDADMAARAEETRARRQQSDLDEARIGAQGQSLRDSDTGQP"
BASE COUNT          690 a         1380 c         1334 g          697 t
ORIGIN      
        1 cttgaagagc ttgacgtgcc gcacgtcaac cttggtgccg acgaagtagg cggtctgatc
       61 gccgcaccac gcccccgcag cccccgccag gaacagcaag ggcaagatcg gcttgccaac
      121 cgactgccac gccgaggcgg caccgatgat caccgattcg ctcggcacca cggggatcac
      181 cgcgtcgatc agggacacgg caaagacgga ggggtagatc cacagggact ccaccaagtc
      241 aaggatgccg atttcaagat tgccggacag atggacgagc cagtcccaga tgccactcat
      301 agggacagtc tgacagccac cactgccgca aggcgatccg cgtgttgttg cgccgcttcg
      361 ggagtggcgg cctccaccat gacgcgtatg agtggttcgg tgcccgacgc gcgcaacagg
      421 accctgcccg aatcgcccag ttcagtgcgc acagattcga gttccttgcc aagttccgcg
      481 tcggtatgca cgcgagagcg atccacccca gtcacgttga tcaatacctg cggcagcgtc
      541 tggacaccgc gcgccaattc cgcgagcgag ccactctttg cgacgagcga cccaagcagg
      601 agggacgtga ggacaccgtc gccggtggtc gcatagcggg acacgatcac gtgacccgac
      661 tgttcgccgc ccaacgtgta gccgccctct cgcatggcct cgagcacgta tcgatccccg
      721 actgccgtct ccacaatgtc gatctctgcc tcgcgcatcg cgtggtgcaa gcccaagttc
      781 gacatgacgg tggcgaccat agtgtcccgg tagagcttgc ctgcgtcgcg catggcgatc
      841 gcgagcaggc ccaagatgtg gtcgccattg acgattgcgc cggcctcgtc cactgcaagg
      901 cagcggtcgg cgtctccatc aaaggcgaca cccatgtgtg cgccctcgtt caccacggcg
      961 gcggccaagg gctcaaggtg cgtagagccc acgccggcgt tgatgttgcg gccatcgggt
     1021 gcgcaatgaa gtcgtaccac cgtggcaccc gcagcctcca gggccgccgg tccgaccacg
     1081 ctcgccgcgc cgtgcgcgcc gtcaacgacg atcgtcaggc ccttcagcgg agactcagcg
     1141 cccccaaact cttggaccgc gtgcacaagg tgcgccagat agtcggccgc aagctcatcg
     1201 gaggcgatga cttcgcccac gtcgccgccg gtgggacgct cccacggctc gcccacgcgc
     1261 gcctcgatcg cctcttccac agcatcggga agcttgacgc ccccgcgcgc gaagaacttg
     1321 atgccgttgt cgggcatcgg attgtgagaa gcggagatca cgacgccgag atcggcctca
     1381 acggaacccg tgaggtatgc gatccctggg gtcggaaaga ctcccaggtc tatgacgtcg
     1441 acgcccgccg aggcgagccc tgccgccact gctgacgaca ggaactggcc cgacaggcgg
     1501 gtgtcacgcc cgagcaccgc ccgcggacga tgcccggaga actcgccgcg ctcggccagc
     1561 acgtgagcgg cagcgaccgc gaggtcgagt gccaactccg ccgtgatgtc gcgattcgcg
     1621 acaccacgga ctccgtccgt gccgaacaga cgcgccatgt gcgcggctct gaactaacgc
     1681 ttcgagtact ggggagcgcg gcgggccttc ttgagaccag ccttcttgcg ctcgaccgca
     1741 cgggggtcgc gggtcaggaa gcctgacttc ttgagcgtcg cacggttggt gtcgaggtcg
     1801 attccgttga gtgcgcgcgc cacaccgagg cgcagtgcgc cggcctggcc ggaagggcca
     1861 ccgccatgga tgcgtgcatg gacgtcgaac ttctcctcga tgtccaagac cgcgaacggg
     1921 gagttcacga tctgctggtg caccctgttg gggaagtagt gctccagctc gcgaccgttg
     1981 atcagccact tgccggtgcc gggcaccaag cgaacgcgtg caacggcctc cttgcgacgg
     2041 cccaggcctg cgccgggtgc aatctcggaa gtgcccttgc cgcgcactgg cgcggtctcg
     2101 gaggtgtact ccgtgagagt ttcggtcgat tcagtggcgt cagtcgacgc ggtggtctcc
     2161 ttcgactcag tggccttggg cgacgcggcg gccttggggg acgcggcggt ctccttcgac
     2221 tcagcggtct tgggggactc agtggtctcc ttcgactcag tggccttgga gggctcagcg
     2281 gtcttggggg actcagtggt cttgggggac tcagtggtct cagtggtctc gtctgccatc
     2341 tcgatgtgtc cttctgcggc gcctactgcg ccacctgcga aatctcgaac ggggcgggct
     2401 gctgcgccgc gtgggggtgc tcagggcctg cgtagacctt cagcttggac agctgggcgc
     2461 gaccgagctt ggtccggggc agcatgccgc ggaccgcgtt ctcgatgacc ttggtgggct
     2521 gatcggcgat cagttcaccg atcggcttgg tcttcagacc gcccgggaaa cccgagtggc
     2581 ggtagaccat cttgtcggtg agcttggcgc ccgtcagggc caccttctca gcgttgatga
     2641 cgatgacgaa gtcaccgcta tcgacgtgcg gagcgaaagt cgccttgtgc ttgccgcgca
     2701 acagcgcagc ggcctgagag gccagacgac cgagaaccac gtcattagcg tcgatgatgt
     2761 gccatttgct ggtcaaatca cccggcttgg gagaaaacgt acgcactatg aagccttcta
     2821 ctctagaaaa cggtggcgcg atgcagtact gcatcgcaag gtcggcgggt cctgcggcgg
     2881 cgagtgggat gcaccggtca cgtggaacgc acaacgaccg gcaagtctac ctgtcgattt
     2941 gccccttggt caatttgggt tcgggaggtc atggctgtcc cgtgtccgag tctcgcagac
     3001 tctggccttg tgcgccgatc ctcgcctcgt cgaggtccga ttgctgtcgc ctcgcacggg
     3061 tctcttcggc gcgtgccgcc atgtcggcat cgccggggta cacaacctcc tcgagcgtca
     3121 gtccccgagg cgccgccacc ttgatcgcgg gagaacgcac ggggtgagtc gagaccattt
     3181 ccagccacac caggtcgcgg cgaccgtagc cgaccgccat cacggctccc accagcgacc
     3241 gcaccatcgc gtggcagaac gcatcggccc tcacggtggc gacgagcagg cccgcgtccg
     3301 cgccggattc caccctctgc cacgagaact ccttgagctc gcggatcgtc gtggcgccct
     3361 cgcggccccg acaaaacgcc gtgaagtcgc gcaaacccag caacgtagcc gaggcggtgt
     3421 tgagggcatc cacgtcgagg gatcggtcga tccacgccac gtgaccgcgg cgcagcgggt
     3481 ctcgtaaggc acgtcgatcg cagatgcggt actcgtagtg ccgctccaag gccgagaagc
     3541 gggcgtcaaa gccctcgggt gcctccgagg cggcccggat gacgatgtcg gcacccacca
     3601 cccctcccag gcgctccctg agggcgtgag cgggcgtgcg ggaggtcttg cccggcagcc
     3661 gcctccacgc aacggccgga atgtcgacgt gcgccacctg accgcgcgcg tggaccccgc
     3721 cgtcggtgcg ccccgcgacg gtgatgcgca gcgtcggttc gtcgtaggag cgacctgccg
     3781 ggccgcgcac cacccggacc agccctgcct ccaactcccc ttgcaccgtg cggaggcccg
     3841 gctgcgtacc ccagccactg aacgcgccgc cgtcgtaaga aaggtcgagg cgggccctca
     3901 ccacctcgcc gctgtgcagc tcagtcatgt ggacccgcct cgtgcgcggt aaaggcgcga
     3961 attacttgtc gtcggcgtcg gaggccttgg tggcctcgtc agctgcggcg gtctcgtcga
     4021 tgatctcctc gatgacggac tcctcagcgg gttcaatctc gagaacctct gccttggggg
     4081 ccttggcctt gggtgctgcc t
//
LOCUS       DASRKH010000401         2648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_65802, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000401 DASRKH010000000
VERSION     DASRKH010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65802"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VFC06_06495"
     CDS             <1..261
                     /locus_tag="VFC06_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81566.1"
                     /translation="HHASGARGESREHRSQAQNKRAALERLVQTHQFRYWVREHIREI
                     DGKESAEQWVERQLADIDNLKVEVKDAEGRWTTTTMEELSAE"
     gene            258..1001
                     /locus_tag="VFC06_06500"
     CDS             258..1001
                     /locus_tag="VFC06_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81567.1"
                     /translation="MTRPKRYNAATLPGFEAAALLLRDEPGLGRLELSRRLNVAPATA
                     INWLGGPLVQFAVGSDRTGTTRSSVFSDEDVLAALRAAAADIPPGTGLSVGHYNAARR
                     RLEDGDIEFPSTALIVQRFSTWRAALTLAGVATNRSNRSEYVRRWSEDEMTLAVADSI
                     IATGTSAYEAYREWARGADAPQQVRISSERSWNGLVEAAYREFADGGRRRTQYQKRLR
                     GLVAARRDELEGVDNMQHGNTPTAEGAQR"
     gene            998..>2648
                     /locus_tag="VFC06_06505"
     CDS             998..>2648
                     /locus_tag="VFC06_06505"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HZL81568.1"
                     /translation="MNIVLKGYQEDAVARCLKRVTRAAEDWRDDMAQSAVSLFATTSA
                     GKTIIATALIEQLRFGDNDRDPNPNMTVLWLTNSPPLNRQTMMKMRATSTHIKEHHLV
                     EIDDQISGATLAPGNIYFLNTQKLNARSTSFQASESRPVSLEQMIRNTVDLLGPDFLL
                     VIDEAHIGTKAGEGGKEQVAYRVMNGVDGSGQFPTVLGISATPKRFRDATTAAGRGLQ
                     TVEVPVNEIRESGLIKDRILAHVPDDDQYTESKFIRAAVEKWQEATEDWREYHQSDPA
                     TADVVPLLLVQVPPGVSDDRLSGLLREIEEHDARLSGAAIAHAFGDPKGPLHLGDRKV
                     NFIAPEDIQGATHIRVVLFKDALTTGWDCPRAEVLVSLRTAKEQTYITQMVGRMVRTP
                     LARRIEGSPSLNDVTMYLPLYNLDALETVRKALSSGEETAVASEFVTKPVSVHLVAGR
                     TDNVLEGDEVVERLRSIVSHRRPGRREPAIVRLGRLAALMERVSTSDNGVPKNAESLA
                     NSALADAMFGEYEEHQDEIDARVAEYHQVGVTVVDIGDGAGG"
BASE COUNT          528 a          835 c          873 g          412 t
ORIGIN      
        1 caccacgcat ccggcgcccg cggggagtct cgtgagcacc gttcgcaagc ccagaacaag
       61 agggcagcac tcgaacgtct cgtgcagacg caccagttcc gctactgggt gcgggagcac
      121 atccgtgaga tcgatggcaa ggaatccgca gagcaatggg tcgagcggca actcgccgat
      181 atcgacaact taaaggtcga agtcaaggac gccgaagggc gctggacgac cacgaccatg
      241 gaggagttga gcgcagaatg acccgaccga agagatacaa cgctgcgacg ctgcccgggt
      301 tcgaggccgc cgcgctgctg ctacgagacg agccaggact tggacgtctc gaactgtcga
      361 ggcggctcaa cgtcgcccct gcgactgcca tcaactggct cggtggacca ctggtgcagt
      421 tcgctgtcgg ctcggaccgt acgggcacaa cgcgttcgtc ggtgttttcc gatgaggatg
      481 tgctcgccgc gctgcgagcc gcggccgcag atatccctcc cggcacgggg ttgtcggtgg
      541 ggcactacaa cgcggctcgg cgccgtctcg aggacggcga tatcgagttc ccgagcaccg
      601 cactgatcgt tcaacggttc agcacatggc gcgccgcact cacgcttgcg ggcgtggcga
      661 cgaatcggtc gaaccggagt gagtacgtca ggcgatggtc ggaagacgag atgacgcttg
      721 ccgtcgccga ctcgatcatc gctacgggga catctgccta tgaggcatac cgagagtggg
      781 cacgaggagc agatgcaccg cagcaggtgc gaatctcctc cgagcggtcg tggaacgggc
      841 tggtcgaagc cgcgtaccga gagttcgccg atggcggccg ccgtcggacg cagtaccaga
      901 agagacttcg cgggctggta gcagcacgac gcgacgagtt ggaaggcgtg gacaacatgc
      961 agcacggcaa cacaccgaca gcagagggag cacagcgatg aacatcgtat tgaagggtta
     1021 ccaggaggac gctgtcgctc gctgcttgaa gcgtgtcacc cgtgccgcgg aggactggcg
     1081 ggacgatatg gcacagtccg ctgtgtcact gttcgccacc acctcggcgg gcaagacaat
     1141 catcgcgacg gcgctcatcg agcaactgcg cttcggagac aacgaccgcg acccgaaccc
     1201 gaacatgacg gtgctgtggc tgacgaactc gccaccgctg aaccggcaga cgatgatgaa
     1261 gatgcgcgcg acgtcgacgc acatcaagga gcaccacctg gtcgagatcg acgaccaaat
     1321 ctccggtgcc accctcgctc ctggcaacat ttacttcctg aacacccaga agttgaatgc
     1381 gcgatcgacc tcgttccagg cgtcagaatc acggcctgtg tcgctcgaac agatgatccg
     1441 caacacggtc gacttgctcg gcccggactt cctgctcgtc atcgacgagg cccacatcgg
     1501 caccaaggcg ggggagggcg gcaaggaaca ggtcgcctac cgcgtcatga acggggtcga
     1561 cggctccggc cagttcccca cagtcctcgg catctctgcg acacccaagc ggttccgtga
     1621 cgccaccacg gccgccggtc gtggcctcca aacggttgag gtgcccgtca acgagatccg
     1681 cgagtcgggg cttatcaagg accgcatcct ggcccacgtg cccgacgacg accagtacac
     1741 ggagtcgaag ttcattcgcg cggcagtcga gaagtggcag gaggcgaccg aggactggcg
     1801 cgagtaccac cagagcgacc ccgcgaccgc cgatgtggtc ccactgctgc tggtgcaggt
     1861 gccgccgggt gtctcggacg accgtctgtc cggtctgctg cgcgagatcg aagagcatga
     1921 cgcacggctg tcgggcgcgg cgatcgcgca cgcgttcggt gaccccaagg gtccgctgca
     1981 cctcggagac cggaaggtca acttcatcgc tcccgaggac attcaggggg cgacacacat
     2041 ccgtgtggtg ctgttcaagg acgccctgac gacaggttgg gactgccctc gtgccgaggt
     2101 gctggtgtcg cttcgtactg ccaaggagca gacctacatc acgcagatgg taggacgcat
     2161 ggtgcgtaca ccgctggcgc gccgtatcga aggctccccg tcactcaacg acgtcaccat
     2221 gtatctgccg ctgtacaacc tggacgcgct cgagacggtg cgcaaagcgc tctcctcggg
     2281 cgaggagacg gctgtcgcgt cggagttcgt cacgaagccg gtcagtgttc acctggttgc
     2341 cgggcgcacg gacaacgtcc tcgaaggcga cgaagtggtc gaacgcttgc ggtcgatcgt
     2401 ctctcaccgt cgacccggcc gacgcgagcc ggccatcgtg cggctcggac gactggccgc
     2461 cctgatggag cgggtcagca cctccgacaa tggggtgccg aagaacgccg agagtctcgc
     2521 caactctgcg ctggcggacg ccatgttcgg cgagtacgag gagcaccaag acgagatcga
     2581 tgcgcgcgtc gccgagtacc accaggtggg cgtcaccgtc gtcgacatcg gagacggggc
     2641 cggtggtg
//
LOCUS       DASRKH010000402         2411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_56463, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000402 DASRKH010000000
VERSION     DASRKH010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56463"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..912
                     /locus_tag="VFC06_06510"
     CDS             130..912
                     /locus_tag="VFC06_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015772383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZL81569.1"
                     /translation="MGRHAATSAEPSPLAADAVATARHLTKIYGSGETKVVALDDVDV
                     DFGRGRFTAIMGPSGSGKSTLMHCMAGLDSPTSGTIVVDGLEISGMTQRELTKVRRNR
                     IGFVFQAYNLIPTLTARENITLPLDIARAEVDQSRFDQVVDAVRLRDRLHHRPSELSG
                     GQQQRVACARALVGNPSVVFADEPTGNLDSRAAAEVLNFLRHSVDELGQSVVMVTHEA
                     TAASYADRILFLADGRIVHELLDPTRDSVLATLAELTPAEVH"
     gene            912..>2411
                     /locus_tag="VFC06_06515"
     CDS             912..>2411
                     /locus_tag="VFC06_06515"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZL81570.1"
                     /translation="MFRIALRDIRTHWVRFILSILAVLLATAFVAGTFTLTALLGTTI
                     EQLSTSTANADVYIRGPETDSATGGSPFGSSRGTVPLDLAAQAEAVDGVAAARPDLFG
                     MAYVVGKDGLAVTNSRAPSFGWVLWPEFDTATVLAGRLPDGENEIALDTKTLEKSGYQ
                     VGDTAPLSIGQIGTREVTVVGEVEYDIPLLGATILVISADLGEAAFAATGEVSQISVK
                     VIDGADPQVVADAVQAELGSSVEVVTGETIREEARTATQDALGFIEIFILVFALVAMF
                     VGSFLIFNTFAMLVRQRQREFALLRAIGASPIQVVLTLLVQAAVVGLVGAVGGIGAGI
                     ALIIGLQKVLTNLGIDFGGNAISLPTERALAIVVAAVVVTCLAAVIPARDAANTAPVE
                     AMRPDSPKAEKSLVLRAIVSGVLLVAGIGAVVYAVLQLRGIALGIGAFAVLLGVLGMA
                     PVMVPAVLGTLGLPLRRIKPVGTLARGNVVRNPRRTAATAAALTIGMALV"
BASE COUNT          329 a          905 c          809 g          368 t
ORIGIN      
        1 ccggtcgacg aggagcgccc ggatcagccg tgagcacaat cgcccacggc ggcgggagcg
       61 cgtgatttcg ccaacgtaga gttggggcag caccgtttcc gggcactcct gctagtgaag
      121 agctgaacga tgggcagaca cgccgccacc tccgccgaac cgtcgccgct cgccgcggac
      181 gccgtcgcca ccgcgcgcca cctcaccaag atctacggca gcggggagac gaaggtcgtc
      241 gcgctcgacg acgtcgacgt ggacttcggc cgcggccggt tcaccgcgat catggggccg
      301 tccgggtcgg ggaagtccac cctcatgcac tgcatggctg ggctggactc cccgacgtcc
      361 ggcaccatcg tcgtcgacgg gctggagatc tccggcatga cccagcggga gctgacgaag
      421 gtgcgccgca accgcatcgg cttcgtgttc caggcctaca acctgatccc cacgctcacc
      481 gcacgggaga acatcaccct cccgctggac atcgcccgcg cggaggtgga ccagtcccgc
      541 ttcgaccagg tggtcgacgc cgtccggctc cgggaccgcc tccaccaccg tccctccgaa
      601 ctctccggcg gccagcagca gcgcgtggcg tgcgcccgcg cgctggtggg caacccgtcg
      661 gtggtgttcg ccgacgaacc caccgggaac ctggactccc gggcggccgc cgaggtgctc
      721 aacttcctgc ggcactcggt ggacgagctg ggccagtcgg tggtcatggt gacccacgag
      781 gccaccgcgg cctcctacgc ggaccgcatc ctgttcctcg cggacggccg gatcgtccac
      841 gagttgctcg accccacccg cgattcggtc ctcgccaccc tcgccgaact caccccggcc
      901 gaagtccact gatgttccgg atcgcccttc gtgacatccg cacgcactgg gtgcggttca
      961 tcctctccat cctcgccgtg ctgctggcca ccgcgttcgt ggccggcacg ttcaccctga
     1021 ccgcactgct cggcaccacg atcgagcagc tcagcacctc caccgcgaac gcggacgtct
     1081 acatccgggg tcccgagacc gattcggcga ccggcggcag cccgttcggc tcgtcgcgcg
     1141 gcacggtccc tctggacctc gccgcgcagg cggaagcggt cgacggcgtc gccgcggccc
     1201 ggccggacct gttcggcatg gcgtacgtgg tggggaagga cgggctggcg gtcaccaact
     1261 cgcgcgcgcc gtcgttcggg tgggtcctgt ggcccgagtt cgacaccgcc acggtcctcg
     1321 cgggccgcct gcccgacggc gagaacgaga tcgcgctgga caccaagacc ctggagaagt
     1381 ccggatacca ggtgggggac acggccccgc tgtcgatcgg ccagatcggc acgcgcgagg
     1441 tcaccgtggt cggtgaggtc gagtacgaca tccccctcct cggcgccacc atcctcgtca
     1501 tctccgcgga cctcggcgag gcggcgttcg cggccacggg cgaggtctcg cagatctccg
     1561 tcaaggtgat cgacggcgcc gacccccagg tggtcgccga cgccgtccag gcggaactgg
     1621 gcagctcggt cgaggtggtc acgggcgaga cgatccgcga ggaggcccgc accgccaccc
     1681 aggacgccct cggcttcatc gagatcttca tcctcgtgtt cgcgctcgtc gccatgttcg
     1741 tgggctcctt cctgatcttc aacacgttcg cgatgctggt gcgccagcgc cagcgggagt
     1801 tcgccctcct gcgagccatc ggtgcctcgc cgatccaggt ggtgctgacg ctgctggtgc
     1861 aggccgccgt cgtcggcctc gtgggcgctg tcggggggat cggggccggg atcgccctga
     1921 tcatcggact gcagaaggtg ctcaccaacc tcggcatcga cttcgggggc aacgccatct
     1981 cgctgcccac cgagcgcgcg ctcgcgatcg tggtcgcggc cgtcgtcgtc acgtgcctgg
     2041 ccgccgtcat cccggcccgc gacgccgcga acacggcgcc cgtggaggcc atgcggccgg
     2101 acagccccaa ggccgagaag tccctcgtgc tgcgcgcgat cgtctccggc gtgctgctgg
     2161 tggccgggat cggcgccgtc gtctacgcgg tcctgcaact tcgcggcatc gcgctcggga
     2221 tcggggcctt cgcggtcctg ctcggcgtgc tcggcatggc cccggtcatg gtccccgctg
     2281 tcctgggcac cctcgggctg ccgctgcggc gcatcaagcc ggtcgggacg ctcgcccgcg
     2341 ggaacgtggt gcgcaacccg cgccgcaccg ccgcgaccgc cgccgccctc accatcggga
     2401 tggccctcgt g
//
LOCUS       DASRKH010000403         2372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_15981, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000403 DASRKH010000000
VERSION     DASRKH010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15981"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VFC06_06520"
     CDS             <1..315
                     /locus_tag="VFC06_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81571.1"
                     /translation="ALDDAAAAGAEPAVVESLRSRAREWGERVDGVCGPAQSGAAAEL
                     TAVTPQRGRNSRTLSAPPPLRKQDVTAAKTCNTYIARTRTVCVLPEGHPGWPKTGHRS
                     KR"
     gene            547..981
                     /locus_tag="VFC06_06525"
     CDS             547..981
                     /locus_tag="VFC06_06525"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZL81572.1"
                     /translation="MMTTAIRDSRLHLGLSQADLAQRLRVTRAAVQQYERNEERGVIS
                     LATLRRALAALGGSVVVQAAGVEDVPKRLFLDTAERLAREVVDARTRQSVSVSELSAQ
                     AEVPATLIEAMERGSAVYDLSAIVRVLKVLGIEPLALPSPPP"
     gene            1048..>2372
                     /locus_tag="VFC06_06530"
     CDS             1048..>2372
                     /locus_tag="VFC06_06530"
                     /inference="COORDINATES: protein motif:HMM:NF019421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HZL81573.1"
                     /translation="MPALHVWYRGAIAGEFVREGSAVSLRYDSGYVGPPLSLSLPKEG
                     QPAEKAAGHYLDNLLPDNPRVRNRWARELRVPSSPFDILGHMGEDLAGAFSIVPEGQG
                     PDSRRGALVAISEDELHHRAASIQNDQDLWLPTDQIGKVRMSLAGAQGKFSLVSVGDM
                     WFFSTISSPSTHIFKPAAKRFPELASLEAASLAIASTVGVRAPMAEAADLGSPRGTFV
                     VERFDRTTDPSTGRTVRVHAEDMAQAHGRRPADKYAIPARDVVSMLGRHARDKDAPYE
                     FVEMLALNVAIGNADAHGKNYSVMIEDDGKAVLAPIYDTVPTAYYPELNTRLGMKVGR
                     AHDGRNVQVGDWQKFAADARLDTERVVEVARRIHEGVAEVAHGEYKAAGVTGAALERM
                     DAIVAERTQILRPIGRSGSTSNRARGARPHSFEGKAETGASTECSARTA"
BASE COUNT          397 a          750 c          815 g          410 t
ORIGIN      
        1 gcgcttgacg atgcagccgc tgctggcgcc gagcccgctg tggtcgagtc cttgcgctct
       61 cgagcccgcg agtggggcga gcgggtcgat ggcgtgtgcg ggcctgcgca gtctggtgct
      121 gccgcggagt tgaccgccgt cacgcctcaa cggggcagga actcccggac gctttcggct
      181 ccgccacccc tgcgaaagca ggatgtgacg gccgcgaaga cgtgcaacac ctacatcgct
      241 cgcacgagga cggtgtgcgt gttgccggag gggcaccccg gctggccaaa aacggggcac
      301 cgctccaagc gctgaccgac agccgtacga gagcgagacg ctcttgaacc caccgacaag
      361 acgatgagga tgatcatgat gaagatcgcg atgcgattga tgtcagccgc cgcgtccggg
      421 cccgcctagc cctagacgcc tacgcgcttt ccgcacgcgt ttcgccacct tgcgccgagt
      481 ccttccaaca cttgaccccg ccgttcgtgt gtgtgatgat agttgtaact atccatacgg
      541 gagggcatta tgacgaccgc gatcagagac tcccgactgc acctcggtct gtctcaggcg
      601 gaccttgccc agcgccttcg agtgacgcgc gctgcggtcc agcagtacga gcgcaacgag
      661 gagcgtggcg tgatctcttt ggccacgcta cgtcgcgctc tcgcggcgct gggcgggagc
      721 gtggtggttc aggcggcagg cgtcgaggat gttccgaagc gtttgttctt ggatacggcc
      781 gagcgtctcg ctcgggaagt ggtcgacgct cgcacgaggc aatccgtgag cgtgtccgag
      841 ttgtccgcgc aggcggaggt tccggcgacc ctcatcgagg cgatggagcg tgggtctgcg
      901 gtgtatgacc tctccgcgat cgtccgggtc ctcaaagtgc tcggcatcga gccgctggcc
      961 cttccgtccc cgccgccgtg aacggcgacg agacccgttg gatacctaac actcacagac
     1021 gcaccgcggt cggaaggaat gcttgagatg ccagcgcttc acgtttggta ccgaggcgcg
     1081 atcgccggcg agtttgtccg cgaaggctcg gccgtgtcgc tgcgctatga ctccgggtac
     1141 gttgggcctc cgctgtcgct gtcgcttccg aaggagggtc agccggcaga aaaggcggcc
     1201 ggtcactatc tcgacaattt gctccctgac aacccgcggg tgcgcaaccg ttgggcccgc
     1261 gaactcaggg tgccctcgtc cccgttcgac atcctggggc acatgggcga agacctcgct
     1321 ggcgcgttct cgatcgtgcc ggagggtcag gggcccgact cgcgccgcgg cgcgctcgtg
     1381 gcgattagcg aagacgaact gcaccaccgt gccgcgtcca ttcagaacga ccaagacctt
     1441 tggttgccga cggaccagat cggcaaggtc cgcatgtcgc tcgccggtgc gcaggggaag
     1501 ttctcgctgg tgagcgtcgg ggatatgtgg ttcttctcga ccatttccag cccgtcgacg
     1561 cacatcttca agccagcggc gaagcgcttc ccggaactgg cctctctcga ggcggcctct
     1621 ctcgcgatcg cgtcgactgt tggcgttcgc gcgcccatgg ccgaggccgc ggatctgggc
     1681 tctcctcgcg ggacgttcgt cgtcgagcgg ttcgatcgga ccaccgaccc gagcacgggg
     1741 cgcacggtgc gtgtgcatgc ggaagacatg gcgcaggctc acggtcggcg ccccgcagac
     1801 aagtacgcga tccctgcgcg tgacgtggtg tcgatgctgg ggcgccacgc gcgagacaag
     1861 gacgcaccgt acgagttcgt cgagatgctg gcgctcaacg tggcgatcgg aaatgcggat
     1921 gctcacggca agaactacag cgtcatgatc gaagacgacg gcaaggcggt gcttgctccc
     1981 atctatgaca cggtgcccac ggcctactac ccggagttga acacgcggct gggaatgaag
     2041 gtgggccgcg cgcacgacgg gcgcaatgtc caggttgggg actggcagaa gttcgccgcg
     2101 gatgcccgcc tcgacacgga acgcgtcgta gaggttgccc gccgcattca tgagggtgtc
     2161 gccgaggtgg ctcatgggga gtacaaggcc gcgggcgtga cgggtgcagc ccttgagcgc
     2221 atggacgcga tcgtggccga gcgcacgcag atacttcgac cgatcggccg ttccgggtcg
     2281 acaagcaacc gcgcgcgggg tgctcgaccc cattcgttcg aggggaaggc ggagactggc
     2341 gcgtcgacag aatgcagtgc ccgcacggcg ga
//
LOCUS       DASRKH010000404         2453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_6733, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000404 DASRKH010000000
VERSION     DASRKH010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6733"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="VFC06_06535"
     CDS             <1..923
                     /locus_tag="VFC06_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HZL81574.1"
                     /translation="GTLVIKEGMPWGVGVAVGIIVGLLAGAWQGFWVAFVGIPAFITT
                     LAGMLLFRGATWYVLSSISLSPFPNAYQDIGNGFANGWLGGNGYDLFTVLIFAFAIVG
                     YAVNSVRGRQARIAYNQSVESVGLMVLKIAAVGAVVMAFAWQLAQDRGLPFVLIILGV
                     LIMVYTVVTTRTVFGRHVYAIGGNLTAATLSGVKVKWVNFGILLNMGFLAGVAGILYS
                     ARTNSAQPAAGNLFELDAIAAAFIGGAAVTGGVGRVTGAMVGGLIMAVLSNGMSLMSL
                     GLPEQQMVKGLVLLLAVAFDVSNKRRAGTK"
     gene            complement(1042..1707)
                     /locus_tag="VFC06_06540"
     CDS             complement(1042..1707)
                     /locus_tag="VFC06_06540"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZL81575.1"
                     /translation="MTQKEAKAQFPDAHGIVVGELDSLISTSALVAKATAFGPYDVII
                     HNAGIGAGVAERAQTADGFERIFHTNVIAPYMLTALMELPRRMIFLTSGLEAKGAWSP
                     EDLQWTNRSWKGMRVYSDSKLHDVMLALEVAARYPQVISNAVDPGWIKTDMGGPSAPD
                     PVALGAETQVWLATSDDADATASGRYLKRRQVLDPNPVALDGNARTQLVDELARLSGV
                     TRP"
     gene            1760..2248
                     /locus_tag="VFC06_06545"
     CDS             1760..2248
                     /locus_tag="VFC06_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HZL81576.1"
                     /translation="MGITDELLEHNAAYAAEYIPDRPLAPQRHLAVVACMDSRLDTFA
                     MLGLDIGDAHVIRNAGGVVTDDVIRSLTISQRKLGTREIILIHHTNCGALTFTDDELR
                     NELLDETGLKPTWSPESFKDLDADLRQSMARLQRCPFLVDTTQVRGFVFDVHSGRLRE
                     VL"
BASE COUNT          378 a          783 c          821 g          471 t
ORIGIN      
        1 ccgggacgct ggtcatcaag gaaggcatgc cgtggggcgt tggcgtggcc gtcggcatca
       61 tcgtgggcct gctggccgga gcgtggcagg gcttctgggt cgcgttcgtc ggcattccgg
      121 cgttcatcac gacgctggca ggcatgctcc tgttccgcgg cgccacgtgg tacgtgctca
      181 gctcgatctc gctgtcgccg ttcccgaacg cctaccaaga tatcggtaac ggcttcgcga
      241 acggatggtt aggcggcaac gggtacgacc tgttcacggt tctgatcttc gcgttcgcga
      301 tcgtggggta tgcggtgaat tccgtgcgcg gccgtcaggc gcgcatcgca tacaaccaga
      361 gcgttgagtc cgtcgggctc atggttctca agattgcggc cgtgggcgcc gtggtgatgg
      421 ccttcgcgtg gcagctcgct caggaccgcg gcctgccgtt cgtgctcatc atcctgggcg
      481 tgctgatcat ggtctacacg gtcgtgacga cgcgcacggt attcggccgc cacgtctacg
      541 cgatcggtgg caacctgacc gccgcgacgc tgtctggcgt caaggtcaag tgggtcaact
      601 tcggcatcct gctcaacatg ggcttcctcg ccggtgtcgc cggaatcctg tactcggcac
      661 gcaccaactc cgcgcagcct gcggcgggta acctcttcga gctcgacgcg atcgccgcag
      721 ccttcatcgg tggtgccgcc gtgaccggcg gcgtgggacg cgtgaccggc gccatggtcg
      781 gtggtttgat catggccgtg ctgtccaacg gcatgtcgct catgagcttg ggccttcccg
      841 agcagcagat ggtcaagggc ctggtgctgc tgctcgcggt ggcgttcgat gtctcgaaca
      901 agcgtcgggc gggaaccaag tagcaagacc tctccggtcg ggcacactct cccgtggtgt
      961 gcccggtggc catgccccgc cgaaccgtgc gcgcagacgc ggttcggcgg ggcatcgccg
     1021 tgtctggccc cggacgtggg actacggacg cgtgaccccc gagaggcgag cgagctcatc
     1081 cacgagttgg gtgcgggcgt tcccgtccag cgcgacgggg tttgggtcga gcacctggcg
     1141 gcgcttgagg tagcgccccg aggcggtggc gtccgcgtcg tctgacgtgg cgagccacac
     1201 ctgggtctcg gcgcccaacg cgaccggatc gggtgcgctc ggcccgccca tgtctgtctt
     1261 gatccatccg gggtcgacgg cgttcgagat cacctgggga tagcgggccg cgacctcgag
     1321 ggcgagcatg acgtcgtgca acttggagtc tgagtacacc ctcatgccct tccacgaacg
     1381 gttggtccac tgcaggtcct cgggcgacca cgcgcccttc gcctcaagtc ccgaggtcag
     1441 gaagatcatg cgccgcggca gttccatcag cgccgtcagc atgtagggcg cgatgacgtt
     1501 ggtgtggaag atgcgctcga agccatccgc cgtctgcgcc ctctccgcga cgcccgcgcc
     1561 gatgccggcg ttgtggatga tgacgtcgta cgggccgaag gcagtcgcct tggcgaccag
     1621 cgcagaggta gagatgaggc tgtccagttc gccgacgacg atgccgtgcg catcgggaaa
     1681 ctgggccttc gcctccttct gtgtcacaac gtgtgcgttg ggctgcgcta tcccggtggc
     1741 gcgggttagc gtatggccca tgggaatcac cgacgagctc ctcgagcaca atgccgccta
     1801 cgcggccgag tacatccccg accgtccgct tgctccgcag cgccacctcg cggtggtggc
     1861 ctgcatggac tcgcggctcg acacgtttgc gatgcttggt ctcgacattg gtgacgcgca
     1921 cgtcatcagg aacgcgggcg gcgtggtcac agacgacgtg atccggtcgc tgaccatctc
     1981 ccaacgcaag ttgggcaccc gcgagatcat cctgattcac cacaccaact gcggcgcact
     2041 gaccttcacg gacgacgaac tacgcaacga actcctcgac gagaccggac tcaaacccac
     2101 gtggagccca gaatcgttca aggacctgga cgcggacctg cgccagtcga tggcgcgtct
     2161 gcaacgctgc ccgttcctgg tggacacgac gcaagtgcgc ggcttcgtgt tcgatgtgca
     2221 ctcggggaga ttgcgcgagg tcctgtaggt tcgctccctt aacctggtgt gaacgaaggg
     2281 cgacaacatg cgcatgtggg gcaagttcct gaaggcgatt ctgaggggcg agtggaagct
     2341 ctcgcccatg tcgtgggttg ccgcgatcgg caccattttc tacgtggtgt cgccgatcga
     2401 cgtgatcccc gagctgtttc tcccgttcat cggctacatc gacgacctcg gcc
//
LOCUS       DASRKH010000405         2921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_65097, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000405 DASRKH010000000
VERSION     DASRKH010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65097"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            317..1714
                     /locus_tag="VFC06_06550"
     CDS             317..1714
                     /locus_tag="VFC06_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012877285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZL81577.1"
                     /translation="MSDPRHLSVAIVGAGPAGIYAADILSKSGLDVSIDIFERLPAPY
                     GLVRYGVAPDHPRIKQIIVALFRILQRGDIRLIGNVEVGRDIPVSQLTERYDAVILAT
                     GSDRDAPINLPGTDLPEVHGASEFVFWYDGHPEAPRTWPLEAKEVAVLGVGNVALDVA
                     RMLTKHVEDLMPTEIPANVAEVLRSSPVTDVHLFGRRGPAQVKFSPLELREMGQMRDV
                     DVIVHEEDFDFDEGSEAAIRSSNQTKQVVKTLVDWTLKDPEDRRASRRIHLHFFHKPV
                     AVLGRGGHVVGIRTERTQLVGDGTVRATGEYVDTPVGAVYRAVGYFSSPVEGVPFDDV
                     RGVIPNSEGRVLDEHDQPIRGLYATGWVKRGPVGLIGSTKSDAQQTIAHLVEDAHAGR
                     LDRDRDQDALGHDAMVADLEAAGIRFTTWQGWELLDAYERALGADEGRDRVKVVERET
                     MIAISRDEGHVATLP"
     gene            1770..2642
                     /gene="htpX"
                     /locus_tag="VFC06_06555"
     CDS             1770..2642
                     /gene="htpX"
                     /locus_tag="VFC06_06555"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HZL81578.1"
                     /translation="MGSSHHNGMKTAALFGVLWAILLGIGWVIAGSTNQPAFIWVFAA
                     FGLVTTFVSYWFSDRIALASMQARPVSEVEAPQIYAIVRELSIRAGQPMPRIHISPTT
                     APNAFATGRNPANAAVCVTRGILEILEPRELRGVLGHELMHVYNRDILTSSVAAAVAG
                     IITSVAQFMLFFGGGGRGREGGNPIGGLLMVILAPIAASMIQLAISRTREYDADEDGA
                     RLTKDPLALASALAKLDRGTQQRPLRPTPATESVSHMMIANPFRGGGGFSRMFASHPP
                     MADRIARLERMAGY"
     gene            complement(2677..>2921)
                     /locus_tag="VFC06_06560"
     CDS             complement(2677..>2921)
                     /locus_tag="VFC06_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF520 family protein"
                     /protein_id="HZL81579.1"
                     /translation="PKPSGKEYRLAGTLREGLNQETAKKITKLIRDQAAKGVKTQITG
                     DEVRVSSKSRDDLQDVIALLQGAEDIEAALQFVNYR"
BASE COUNT          444 a         1043 c          993 g          441 t
ORIGIN      
        1 ggtcccactg ccgcaggacg gtgagggcga ggacgcagaa gaccagggac cacaacgcgc
       61 ggtgggccac gacctcgatc ggccctgagc gctccatcag ccggaagtac agcgggaacg
      121 cgccccacag gaggtaggcc gcgaagccga agatcacgcc agaccgctcc cgcaccagcc
      181 gattctgcca tgcgcgtcgg cgcgccgcgg cgggcgagcg tgtggccaac ctctcggcgc
      241 cccccgcgtc agcgctccgt tgggcttata gaatcatcgg gaagtgcccg cgggcatccg
      301 cattcgaaag gcagtcgtga gcgatccacg ccacctgagc gtcgcaatcg tgggcgcggg
      361 gccggcaggc atctacgccg ccgacatcct gtccaagtcc gggctcgacg tctcgatcga
      421 catcttcgag cggctgccgg cgccgtacgg cctggtgcgc tacggcgtcg cccccgacca
      481 cccgcgcatc aaacagatca tcgtggcgct cttcaggatc ctgcagcgcg gtgacatccg
      541 gctgatcggc aacgtcgagg tcggccggga catcccggtt tcgcagctca ccgagcgcta
      601 cgacgccgtg atcctcgcca ccggctcgga ccgtgacgcc cccatcaacc tccccggcac
      661 cgacctcccc gaggtccacg gcgccagcga gttcgtcttc tggtacgacg gccaccccga
      721 ggcgccgcgc acctggccac tggaggcgaa ggaggtcgcc gtgctgggtg tgggcaacgt
      781 ggcgctcgat gtcgcgcgca tgctgaccaa gcacgtcgag gacctcatgc ccaccgagat
      841 cccggccaac gtggcggagg tgctgcgcag cagcccggtc acggacgtcc acctgttcgg
      901 gcgccgcggc ccggcccagg tgaagttctc gcccctcgag cttcgcgaga tgggccagat
      961 gcgtgacgtg gacgtgatcg tccacgagga ggacttcgac ttcgacgagg gctcggaggc
     1021 cgcgatcagg tcatccaacc agaccaagca ggtggtaaag accctcgtgg actggacgct
     1081 gaaggatccg gaggaccggc gcgcctcccg ccggatccac ctgcacttct tccacaagcc
     1141 cgtggcggtg ctggggcggg gcgggcacgt cgtcggcatc cgcacggaac ggacgcaact
     1201 ggtgggggac gggacggtgc gcgccaccgg cgagtacgtg gacacccccg tgggggcggt
     1261 gtaccgcgcc gtcggctact tcagttcgcc ggtggagggc gtgccgttcg acgacgtccg
     1321 cggcgtgatc ccgaactcgg agggccgcgt cctcgacgaa cacgaccagc cgatccgcgg
     1381 actctacgcc accggctggg tgaagcgggg gcccgtgggc ctgatcgggt ccacgaagtc
     1441 cgacgcccag cagaccatcg cgcacctcgt cgaggacgcc cacgcggggc ggctggatcg
     1501 ggaccgggac caggacgcgc ttggccacga cgccatggtg gctgacctcg aggccgccgg
     1561 catccgcttc accacctggc aggggtggga actcctcgac gcctacgaac gtgcactcgg
     1621 tgcggacgag ggccgcgacc gcgtgaaggt ggtggagcgc gagacgatga tcgcgatcag
     1681 ccgcgatgag ggccacgtcg cgacgctgcc ctgagcggcg acgctccggc ggccggaaca
     1741 cttccgccac ccacgtcgtt gggaatggta tgggttcctc tcaccacaac ggaatgaaga
     1801 cggccgcgct cttcggcgtg ctctgggcca tcctgctcgg aatcggctgg gtgatcgcgg
     1861 gctcgaccaa ccagccggca ttcatctggg ttttcgctgc gttcgggctc gtgaccacct
     1921 tcgtctccta ctggttctcg gacaggatcg cactcgcctc gatgcaggcg cggccggtgt
     1981 ccgaggtcga ggcgccccag atctacgcga tcgtgcggga gctctccatc cgggcgggcc
     2041 agccgatgcc ccgcatccac atctcgccca ccacggcccc gaacgcgttc gcgaccggcc
     2101 gcaaccccgc caacgcggcg gtgtgcgtca cccgggggat cctcgagatc ctcgagcccc
     2161 gtgagctccg cggtgtgctc ggccacgagt tgatgcacgt gtacaaccgc gacatcctga
     2221 cctcgtccgt ggccgccgcc gtcgccggca tcatcacatc cgtcgcgcag ttcatgctgt
     2281 tcttcggcgg gggagggcgc ggtcgtgagg gcggcaaccc gatcggcggc ctgctgatgg
     2341 tcatcctcgc gccgatcgcg gcgtccatga tccagttggc gatctcccgc acccgggagt
     2401 acgacgccga cgaggacggg gcgcgcctca ccaaggaccc gctcgccctg gcgtccgccc
     2461 tggccaagct ggaccgcggg acccagcagc gcccgctccg gccgacgccc gccacggagt
     2521 cggtctccca catgatgatc gccaacccgt tccgcggcgg cggcgggttc tcccgcatgt
     2581 tcgcctccca cccgcccatg gcggaccgca tcgcgcgcct ggagaggatg gcgggctact
     2641 gagccgcgcg gggtactgag ccgcgccgcg tgccccctag cgatagttga cgaactgcag
     2701 ggcggcctcg atgtcctcgg cgccctgcag cagggcgatg acgtcctgga ggtcgtcgcg
     2761 ggacttcgag ctcacccgca cctcgtcacc ggtgatctgg gtcttcacgc ccttggcggc
     2821 ctggtcgcgg atcagtttgg tgatcttctt cgcggtctcc tggttcaggc cctcccggag
     2881 cgttccggcg agccggtact ccttgccgga cggcttgggt t
//
LOCUS       DASRKH010000406         2388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_7489, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000406 DASRKH010000000
VERSION     DASRKH010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7489"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VFC06_06565"
     CDS             complement(<1..468)
                     /locus_tag="VFC06_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZL81580.1"
                     /translation="MKGLTSMKRLACAAAGMMLVLALLPGCAGTTPAAATTAAASGAA
                     SPSGESTSAETTIRIASLKGPTTMGLVKLTSDADAGQGKQDYQATMYGTPDEIVPLVT
                     KGDVDVALLPANLASVLYNKTKGAGGSQIELAAINTLGMLELVEAGNTVKSIAD"
     gene            complement(465..>2388)
                     /locus_tag="VFC06_06570"
     CDS             complement(465..>2388)
                     /locus_tag="VFC06_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HZL81581.1"
                     /translation="PVIVEGSDFIIEADTIIGAIGQSTDTGFLYNDLPVRLNKWGDID
                     VDGFTMQSSESNVFAGGDCVTGPATVIQAVAAGRRAAMAIDEFVTRGYVRPSTEDYAC
                     SRGTLEDLPRAEFEDIPRLVRASMPELRPEARIDSFVEVETGLTEEQARAEAARCLKC
                     GCAKQNHCALRQEASDHGVAFATPLHIRPYSPIVVDHPFIVRDNNKCIGCGRCVAACA
                     EIEGPGVLAFQFHAGRMTVGTTTDLPLGLTDCVSCGQCVRACPCGALDYVRERGDVFM
                     AINDPKKVVVGFVAPAVRSVIAAEYGIPFDEASAFIAGMMRKIGFDKVFDFSFAADLT
                     IMEETTEFLGRVTSGGVLPHFTSCCPGWVNLVERRWPEMIPHLSSCKSPQQMMGATVK
                     NHFAQQAGISLDELYVVSIVPCLAKKYEAARPEFAPEGIRDVDAVLTTTEFIEMAEML
                     RLKPDDITPGEFDAPYARVSGAGVLFGASGGVAEASLRMAVEKLTGEPLVDQLDFHEV
                     RGFEGFKEATVTAGGTTVRVAVISGLNNVDPLVRRIVAGEDVGYDLVEVMACPGGCVN
                     GAGHPVPDRVGEMAARQKVLVNIDQTSRYRKSQENPDILRLYDEFYGEPNSDAAHHLL
                     HTSYAPFRDEPIVPTR"
BASE COUNT          345 a          814 c          839 g          390 t
ORIGIN      
        1 gtcggcgatg ctcttgacgg tgttgccggc ctcgacgagc tccagcatcc cgagcgtgtt
       61 gatcgcggcc agctcgatct gcgacccacc cgctcccttc gtcttgttgt agagcacgct
      121 ggcgagattg gctgggagaa gggcgacgtc gacgtcgccc ttcgtgacca gcggcacgat
      181 ctcgtccggc gtgccgtaca tcgtcgcctg gtagtcctgc ttgccctgac ccgcgtcggc
      241 gtcgctcgtc agcttgacca ggcccatcgt ggtcgggccc ttgagcgacg cgatccggat
      301 cgtcgtctca gcgctggtcg actcgccgct gggcgacgct gcaccggacg cagcggcggt
      361 ggtagccgcc gcgggtgtgg tgccggcaca gccgggcagc agcgccagca ccagcatcat
      421 gccggctgcc gcgcacgcga gtcgcttcat ggaggtcagg cccttcatcg ggtggggacg
      481 atcggctcgt cccggaacgg ggcgtagctg gtgtggagca ggtggtgggc ggcatccgag
      541 ttgggttcgc cgtagaactc gtcgtacagc cggaggatgt ccgggttctc ctgcgacttg
      601 cggtaccgcg aggtctggtc gatgttgacg agcaccttct ggcgggccgc catctcgccg
      661 acgcggtcgg gcacggggtg cccggcgccg ttgacgcagc cacccgggca ggccatgacc
      721 tcgacgaggt cgtagccgac gtcctcaccg gccacgatgc gccgcacgag cgggtcgacg
      781 ttgttgaggc ccgagatcac ggccacgcgg acggtcgtgc cgcccgcggt gacggtcgcc
      841 tccttgaaac cttcgaaccc tcgaacctcg tggaagtcga gctggtccac caggggttcg
      901 ccggtgagct tctccacggc catgcgcagg gaggcctccg ccacgccgcc cgaggcgccg
      961 aagagcacgc ccgcgccgct gacccgggcg tagggcgcgt cgaactcgcc cggggtgatg
     1021 tcgtcgggct tcaggcgaag catctccgcc atctcgatga actccgtggt cgtcaggacg
     1081 gcgtccacgt cgcgaatccc ctcgggcgcg aactccggcc gggcggcctc gtacttcttc
     1141 gcgaggcacg gaacgatcga cacgacgtac agctcgtcga ggctgatgcc cgcctgctgg
     1201 gcgaagtggt tcttcaccgt ggcgcccatc atctgctggg gcgacttgca gctcgacagg
     1261 tgggggatca tctccggcca ccggcgctcg acgaggttca cccatcccgg gcagcacgac
     1321 gtgaagtggg gcagcacgcc gcccgacgtc acgcgcccga ggaactcggt cgtctcctcc
     1381 atgatcgtga ggtcggcggc gaaggagaag tcgaacacct tgtcgaagcc gatcttgcgc
     1441 atcatgccgg cgatgaacgc cgatgcctcg tcgaagggga tcccgtactc ggccgcgatc
     1501 acgctgcgca ccgccggggc gacgaacccg accacaacct tcttggggtc gttgatggcc
     1561 atgaacacgt cgccgcgctc acgcacgtag tcgagcgcgc cgcacgggca ggcccgcacg
     1621 cactggccgc acgagacgca gtcggtgagc ccgagcggga ggtcggtggt ggtgccgacc
     1681 gtcatccgtc ccgcgtggaa ctggaacgcg aggacgcccg ggccctcgat ctcggcgcac
     1741 gcggcgacgc accgaccgca cccgatgcac ttgttgttgt cgcggacgat gaaggggtgg
     1801 tccaccacga tcggcgagta cgggcggatg tgcaggggtg tcgcgaacgc gacgccgtgg
     1861 tcgctcgcct cctggcgcag ggcacagtgg ttctgcttgg cgcagccgca cttgaggcac
     1921 cgggccgcct cggcgcgggc ctgctcctcg gtcagtcccg tctcgacctc gacgaagctg
     1981 tcgatgcgcg cctcgggccg cagctcgggc atgctcgcgc gcacgagccg ggggatgtcc
     2041 tcgaactcgg cccgcggcag gtcctcgagc gtcccgcggc tgcacgcgta gtcctccgtg
     2101 ctcgggcgca cgtagccacg ggtgacgaac tcgtcgatcg ccatcgctgc gcgacgtccg
     2161 gccgcgacgg cctggatgac ggtcgccgga ccggtcacgc agtcgccgcc cgcgaagacg
     2221 ttcgactccg acgactgcat cgtgaagccg tcgacgtcga tgtcgcccca cttgttcagg
     2281 cggaccggca ggtcgttgta gaggaagccg gtgtcggtgc tctggccgat ggcgccgatg
     2341 atggtgtcgg cctcgatgat gaagtcgctg ccctcgacga tcacgggc
//
LOCUS       DASRKH010000407         2641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_11764, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000407 DASRKH010000000
VERSION     DASRKH010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11764"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="VFC06_06575"
     CDS             <1..929
                     /locus_tag="VFC06_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81582.1"
                     /translation="SSGDAVYAIPVDGGPMGMLYRKDLFDKAGITTPPTTWDEFAADA
                     QKMRDSGSKALFTDFPTNGRAYLQALFAQKGSSPYVYDAAKPTDVSIKLNDQGSKDVM
                     TYWQGLVDKGLVGTDDAFTTDYNTKLVDGSYAVYLAAAWGPGYLSGLSGTDPTAVWQA
                     APIPQWDASSPVQINWGGSTFAVTSQSKDPEAAATVAKEVFGTEEAWKIGIEQGALFP
                     LWKPILESPYFTDLAYPFFGGQKINQDVFLKASQGYKGFTWSPFQNFAYDKLTEEANA
                     AVVQKTKSTDDAIDSLQKTVVDYAKAQGFTVK"
     gene            1008..1976
                     /locus_tag="VFC06_06580"
     CDS             1008..1976
                     /locus_tag="VFC06_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020015992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HZL81583.1"
                     /translation="MSNPTATGAHAALPTDERMPRTAIALNPRAGHDRRRQRFGWVFV
                     APFAVVFTIFLIAPLGYAFWLSLQTKTLAAGTHFSGLGNYKQAFSDPQFLQGIWLVTR
                     FALILIPLQMLVSLIAALVLDNLTTRLSRFARLAIFVPYAVPVVIGALMWGFLYSPRF
                     GPLKSLFGLFGADAPFLLSPQNVFGGLINVVTWQWAGYYMIIIYAALRGIDPSIYEAA
                     RIDGAKGWQIATRIKIPMISSALILILVFALIGTLQFFTEPQVLRPVAAGSINASYTP
                     NIYAFTLAFSYSQFNYASAISFALGAVVFIGSYAFLFLTRKKNGLS"
     gene            1976..>2641
                     /locus_tag="VFC06_06585"
     CDS             1976..>2641
                     /locus_tag="VFC06_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020015993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HZL81584.1"
                     /translation="MAALLVDETTHEAPRQNGRGGSTRQRQPWSHVLLIILAAYFLIP
                     LWWLVVASTKDINGLFTGAHGPMWFDSTFHLVTNIKQLFTQNGGLYWRWLGNSFLYAF
                     TGGVGATVLAVLAGYGFAKYRFRGRNLFFAMLLGSVMVPLTALVIPTFVLLARFHLTN
                     TIWGVILPSLLNPFGVYLMRVYAQDAVPDEMLDAARVDGAGEVRTFFSVALPLLKPAL
                     VTVP"
BASE COUNT          418 a          953 c          801 g          469 t
ORIGIN      
        1 tctcgagcgg cgacgcggtc tatgcgatcc cggtcgacgg cggcccgatg ggcatgctct
       61 accgcaagga cctgttcgac aaggccggca tcaccacgcc gccgacgacg tgggacgagt
      121 tcgccgccga cgcgcagaag atgcgtgact ccggctcgaa ggccctcttc accgacttcc
      181 ccacgaacgg ccgcgcctac ctgcaggcgc tcttcgcgca gaagggctcg tcgccgtacg
      241 tctacgacgc cgccaagccg accgacgtct cgatcaagct gaacgaccag ggcagcaagg
      301 acgtcatgac gtactggcag ggcctcgtcg acaagggcct cgtcggcact gacgacgcct
      361 tcacgacgga ctacaacacg aagctggtcg acggtagcta cgccgtctac ctcgctgccg
      421 catggggccc gggctacctg tccggcctgt cgggcaccga cccgacggcc gtctggcagg
      481 ctgcgccgat cccgcagtgg gacgcgtcct cgccggtcca gatcaactgg ggcgggtcga
      541 ccttcgcggt cacgtcgcag tcgaaggacc cggaagccgc ggccacggtc gcgaaggagg
      601 tcttcggcac cgaggaggcc tggaagatcg gcatcgagca gggcgcgctg ttcccgctgt
      661 ggaagcccat cctcgagtcg ccctacttca cggacctcgc ctacccgttc ttcggcggtc
      721 agaagatcaa ccaggacgtc ttcctcaagg cctctcaggg ctacaagggc ttcacgtgga
      781 gcccgttcca gaacttcgcc tacgacaagc tgaccgaaga ggccaacgct gcggtcgtgc
      841 agaagacgaa gtcgacggat gatgcgatcg acagcctcca gaagacggtc gtcgactacg
      901 ccaaggccca gggcttcacg gtcaagtagt ccgtagggga cgccgccggg tcgggtcgca
      961 ccagacccgg cccggcggcg gacccgtcct cgacactggg agaacccatg agcaacccca
     1021 cagccaccgg ggcgcacgcg gccctgccga cggacgagcg gatgcctcgc accgccatcg
     1081 cgctcaatcc ccgcgccggg cacgaccggc gacgccagcg gttcggctgg gtcttcgtcg
     1141 caccgttcgc ggtcgtcttc acgatcttcc tgatcgcgcc gctcggctat gccttctggc
     1201 tgagcctgca gaccaagacg ctggccgccg gaacgcactt ctcgggactg ggcaactaca
     1261 agcaggcgtt cagcgacccg cagttccttc aggggatctg gctggtgacc agattcgcgc
     1321 tcatcctcat ccccctgcag atgctcgtct cgctcatcgc cgcgctcgtg ctcgacaacc
     1381 tcacgacgcg gctgtcgcgg ttcgcgcggc tcgcgatctt cgtgccgtac gcagtccccg
     1441 tcgtcatcgg cgccctcatg tgggggttcc tctacagccc gcgcttcggt ccactgaagt
     1501 cgctcttcgg tctgttcggc gcggacgcgc cgttcctgct cagcccgcag aacgtgttcg
     1561 gcggcctcat caacgtggtg acgtggcagt gggccggcta ctacatgatc atcatctacg
     1621 cggccctgcg cggcatcgac ccctcgatct acgaggcggc ccgcatcgac ggtgccaagg
     1681 gttggcagat cgcgacccgc atcaagattc cgatgatctc ctcggcgctc atcctcatcc
     1741 tcgtgttcgc gctcatcgga acgctgcagt tcttcaccga gccgcaggtg ctgcgccctg
     1801 tcgcggcggg ctcgatcaat gcgtcgtaca cgccgaacat ctacgcgttc acgctcgcgt
     1861 tctcctactc ccagttcaac tacgcctcgg cgatctcctt cgccctcggc gctgtggtgt
     1921 tcatcgggtc gtacgcgttc ttgttcctga cccgcaagaa gaacggactc agctgatggc
     1981 cgccctgctc gtcgacgaga ccacgcacga ggcaccccgc cagaacggcc gtggcgggtc
     2041 cacgcgccag cgccagccct ggtcgcacgt cctgttgatc atcctggccg cgtacttcct
     2101 catcccgctc tggtggctgg tcgtcgccag caccaaggac atcaacgggc tcttcaccgg
     2161 cgcgcacggc ccgatgtggt tcgacagcac cttccacctc gtcaccaaca tcaagcagct
     2221 cttcacccag aacggcggcc tgtactggcg ttggctgggt aactcgttcc tgtacgcctt
     2281 caccggcggc gtcggcgcga cggtcctcgc cgtgctcgcc ggatacgggt tcgccaagta
     2341 ccggttccgt gggcgcaacc tgttcttcgc gatgctgctc ggctcggtga tggtgccgct
     2401 gaccgccctg gtgatcccca ccttcgtcct cctggcgcgc ttccacctga cgaacacgat
     2461 ctggggagtc atcctgccgt cgctcctgaa cccgttcggg gtctacctca tgcgcgtcta
     2521 cgcgcaggat gccgtcccgg acgagatgct cgacgcggcg cgggtcgacg gagcgggcga
     2581 ggtccgcacg ttcttcagcg tggccctccc gctgctcaag ccggctctcg tcacagtgcc
     2641 g
//
LOCUS       DASRKH010000408         2605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_47424, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000408 DASRKH010000000
VERSION     DASRKH010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47424"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1034
                     /locus_tag="VFC06_06590"
     CDS             <1..1034
                     /locus_tag="VFC06_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HZL81585.1"
                     /translation="STTNGHTYALAWCVLVTALDALAVVVLLRHRRSTVGTWWWMAFI
                     VLLGPVAMGRLDAIIVPVMIVALAVLLSRPRLAAALLTAGAWIKVAPGAILIPLVLAT
                     RRPWRDVVVPAGLVSAFVVLEVAVGGGTSHVASFLTEQGTRGLQIESVGATPWVLAGL
                     FTRSVTRYLNQELVTWEITGPGSNLAVDVLGLALPLGIAMLAGLFWWLHRRAGTGMHL
                     QAFLVRAALLVLLVLIVLNKVGSPQYLAWLAAPVALALALRLPGWRSTAWQVATLAVL
                     TQIVFPLDYGALLNGRVPVSLVLVARNVLLVVLLVQTVRDLVRDSRRSAESYEPPGLV
                     AAAASHASR"
     gene            complement(983..>2605)
                     /locus_tag="VFC06_06595"
     CDS             complement(983..>2605)
                     /locus_tag="VFC06_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HZL81586.1"
                     /translation="GGGVIGRVVVAEGSPFVSFTAVTDTDLTLAVPFTAAAGSGAPTG
                     SALATATVDGTQYAVVAPDGAVAADGVRLSAGQSAAWYAVPDGADGSVRAALTTAAVD
                     PVIGSEVTYGVDDSVARTTIGYRTAHGGPTAYVTMPHQRAGDQPTRSGCGLATYPSVY
                     GELELCTGSVLTSWAPVVAPAGALDLSQVPQDRRDAVRTQLAADVASTPAFPSDTYFG
                     GKALYRAATLVALGRSLGADDVVAPLRDKVVAALREWTQPDGCTQRQARCFVYDAAAH
                     GVIGLTPSFGSDEFNDHHFHYGYFLAAAGILAATDTALAAELAPVMNLLAQDVAAARP
                     SEQLPQLRVFDVYAGHSWASGTAPFADGNNQESTSEAVNAWNGLGLWAKASGQDALGV
                     EATWLASTEAATARAYWTDPDLSEPVYQGFGHQVFALAWGGKRDYATWFSGEPSAMLG
                     IQLIPMNPAAGYLAGDPDRIRANVAEAAPGGYGVQFGDYLLMYLALAGPQDAEHAWAA
                     AQSLPDTAIDDGDSRAYLLAWLAAAATSPGGS"
BASE COUNT          319 a          954 c          955 g          377 t
ORIGIN      
        1 tgagcaccac gaacggtcac acgtacgcgc tcgcgtggtg cgtgctcgtc acggccctcg
       61 acgcgctcgc ggtcgtcgtg ctgctgcgcc accgccgctc gacggtcggc acgtggtggt
      121 ggatggcgtt catcgtgctg ctcggcccgg tcgcgatggg ccgtctcgac gcgatcatcg
      181 tgccggtgat gatcgtcgcg ctcgcggtac tgctctcgcg tccgcggctc gcagcggcgc
      241 tcctgacggc cggggcctgg atcaaggtcg caccgggcgc gatcctcatc ccgttggtgc
      301 ttgcaacccg gcggccgtgg cgcgacgtcg tcgtccccgc gggcctcgtg tcggcgttcg
      361 tcgtgctcga ggtggccgtc ggtggcggca cctcgcacgt cgcgagcttc ctgacggagc
      421 agggcacgcg cggactgcag atcgagtcgg tcggcgcgac cccctgggtg ctcgcgggcc
      481 tgttcaccag gtcggtcacg cgctacctca accaggagct cgtcacgtgg gagatcacgg
      541 gaccaggctc gaacctcgcc gtcgacgtgc tcgggctcgc actcccgctc ggcatcgcga
      601 tgctggccgg gctgttctgg tggctgcacc ggcgcgcggg gaccggcatg cacctgcagg
      661 cgttcctcgt gcgcgcggcg ctgctcgtgc tcctcgtcct catcgtgctc aacaaggtcg
      721 gctcaccgca gtacctcgcg tggctcgctg cgcccgtcgc gctcgcgctc gcgctgcggc
      781 tgcccgggtg gcgctcgacc gcgtggcagg tcgcgaccct cgcggtgctc acgcagatcg
      841 tgttcccgct cgactacggc gcgctgctca acgggcgggt gcccgtctcg ctcgtgctgg
      901 tcgcgcgcaa cgtgctcctc gtcgtgctgc tcgtgcagac ggtgcgtgac ctggtgcgcg
      961 acagccgccg ttccgcggag agctacgagc cgcccgggct cgtggcggca gcggcgagcc
     1021 acgccagcag gtaggcgcgc gagtcgccgt cgtcgatcgc ggtgtccggc agcgactgcg
     1081 cggccgccca ggcatgctcc gcgtcctgcg gaccggcgag cgcgaggtac atcagcaggt
     1141 agtcgccgaa ctgcacgccg tacccaccgg gcgccgcctc ggcgacgttg gcccggatgc
     1201 ggtccgggtc gcccgcgagg taccccgcgg cggggttcat cgggatgagc tggatgccga
     1261 gcatcgcgct cggctcgccg ctgaaccacg tcgcgtagtc gcgcttcccg ccccacgcga
     1321 gcgcgaagac ctggtgcccg aacccctggt acacgggctc ggagaggtcc gggtcggtcc
     1381 agtacgcccg cgccgtggcc gcctcggtcg acgcgagcca tgtggcctcg acaccgagcg
     1441 cgtcctggcc ggacgccttc gcccagagcc cgaggccgtt ccacgcgttg accgcctcgg
     1501 acgtcgactc ctggttgttg ccgtcggcga acggcgccgt gcccgacgcc cacgagtgcc
     1561 ccgcgtacac gtcgaacacc cgcagctgcg ggagctgctc gctcgggcgc gccgcggcca
     1621 cgtcctgcgc gagcaggttc atgaccgggg cgagctcggc cgcgagcgcg gtgtcggtgg
     1681 ccgcgaggat cccggcggca gccaggaagt acccgtagtg gaagtggtgg tcgttgaact
     1741 cgtcggagcc gaacgacggc gtgagcccga tgaccccgtg cgcggccgcg tcgtacacga
     1801 agcaccgggc ctggcgctgc gtgcacccgt cgggttgcgt ccactcgcgc agggccgcga
     1861 cgaccttgtc ccggagcggc gccacgacgt cgtccgcgcc gagcgagcga ccgagcgcga
     1921 cgagggtcgc cgcgcggtac agcgccttgc caccgaagta cgtgtccgac gggaacgccg
     1981 gggtcgaggc cacgtcggcc gcgagctgcg tccggacggc gtcgcgacgg tcctgcggca
     2041 cctggctgag gtcgagggct ccggcagggg cgacgacggg ggcccaggac gtgagcaccg
     2101 agcccgtgca cagctcgagc tccccgtaca cgctcgggta ggtcgcgaga ccgcagcccg
     2161 aacgcgtggg ctggtcgccc gcacgctggt ggggcatggt cacgtaggcg gtcgggccgc
     2221 cgtgcgcggt gcggtagccg atggtcgtgc gcgcgaccga gtcgtccacg ccgtacgtca
     2281 cctcggagcc gatgacaggg tccaccgccg ctgtcgtgag cgccgcacgg acggacccgt
     2341 ccgcgccgtc cggcaccgcg taccaggcgg cggactgccc ggcgctcagg cgcacgccgt
     2401 ccgcagcgac cgcaccgtcc ggggccacga ctgcgtactg cgtgccgtcg accgtcgcgg
     2461 tcgcaagcgc ggaacccgtc ggggcacccg accctgcagc agccgtgaac ggcaccgcga
     2521 gggtcaggtc cgtgtccgtg accgcggtga agctgacgaa cggtgagccc tcggcgacca
     2581 cgacgcgacc gatcacgcca ccgcc
//
LOCUS       DASRKH010000409         2021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_33642, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000409 DASRKH010000000
VERSION     DASRKH010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33642"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..1152)
                     /locus_tag="VFC06_06600"
     CDS             complement(64..1152)
                     /locus_tag="VFC06_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZL81587.1"
                     /translation="MGKPHGIGVVGLGVIAARYLETLVGHPDVAITAVADLDHGRSAE
                     AASGIPGCRALSVADLVADDAVDTVLNLTTPEAHAEIALAALAHGKNVYGEKPLAATF
                     LDALSVIRARGDAWVGGAPDTVLGTGIQTARAAVDSGSIGRPIAAFATWVAPGHERWH
                     PNPDFYYRAGGGPLLDMGPYYLTSLVQLLGPVTRVTGAASRGRGTRTIGSGPRAGQRI
                     PVEVDTHVTGILEHAGGALSTVVLSFDAVASGAAPIEVHGERGSLSVPDPNTFDGTVR
                     LRLLGDQDWAAVAPSAGYQGAERGIGLLDFVRGGRRAGGDLALHVLEIMTALLASASD
                     GVRVPLTTTVERPLLVPLTTRAQWGKPS"
     gene            complement(1125..1832)
                     /locus_tag="VFC06_06605"
     CDS             complement(1125..1832)
                     /locus_tag="VFC06_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HZL81588.1"
                     /translation="MTARTALIVRGGWYGHSPVDATNLFVPFLEEHGFDVQIEDSNET
                     YADEALMARTDLVVQSVTMSEISDQAFVGLAAAVRRGTGLVGWHGGIADSYRANSDYL
                     QLIGGQFATHPSKDPAQRVGDETDNFLTHTVHVTELGRAHEITAGIEDFTLTTEQYWV
                     LHDDLIDVLATTTHPVQPYHPWHRPITSPVVWTRLWGDGRVVVATPGHSLDVLRKTNV
                     RTIIERGILWASRTASE"
BASE COUNT          281 a          712 c          719 g          309 t
ORIGIN      
        1 gtgcttgttc ctctcggtgt gctggctggt cgacgccgag atcgacgccg tgcgccgtcc
       61 cggtcatgat ggcttgcccc actgcgcgcg ggtggtcagg gggacgagca acggacgttc
      121 cacggtcgtg gtgagcggca cccgcacccc gtcgctcgcg gacgcgagca gtgccgtcat
      181 gatctcgagc acgtgcagcg ccaggtcgcc gccggctcgg cgtccgccgc gcacgaagtc
      241 caggagcccg atgccccgct cggccccctg gtagccggcg gacggcgcca ccgcggccca
      301 gtcctggtcg cccagcaggc gcaaccgcac cgtgccgtcg aacgtgttcg ggtccggcac
      361 cgacaggctg ccccgttcgc cgtggacctc gatcggcgct gccccgctgg cgacggcatc
      421 gaagctcagc accacggtcg acagcgcgcc accggcgtgc tcgaggatgc cggtgacatg
      481 ggtgtcgacc tcgacgggga tccgctgtcc ggcccgcggt cccgagccga tcgtccgcgt
      541 tccgcggccg cgcgacgcgg caccggtgac ccgcgtgact gggccgagca gctggacgag
      601 cgaggtcagg tagtacggac ccatgtcgag cagcggaccg cctccggccc ggtagtagaa
      661 gtccgggttg gggtgccagc gctcgtgacc cggtgcgacc cacgtggcga acgccgcgat
      721 cggccggccg atgctcccgg agtccactgc cgcgcgcgcg gtctggatgc cggtgccgag
      781 caccgtgtcg ggggcgccgc cgacccacgc gtccccacgc gcgcggatca ccgacagggc
      841 gtcgaggaac gtggccgcca gcggcttctc gccgtagacg ttcttgccgt gggccagcgc
      901 cgccagcgcg atctcggcgt gggcttcggg ggtcgtcagg ttcagcaccg tgtcgacggc
      961 gtcgtcggcg accaggtctg cgaccgacag cgcacggcac ccggggatgc ccgacgccgc
     1021 ctcggccgac cgtccgtgat cgaggtcggc gaccgcggtg atggcgacgt cggggtgccc
     1081 caccaaggtc tccaggtagc gcgcagcgat gacgccgagc ccgactactc cgatgccgtg
     1141 cggcttgccc acaggattcc cctctcgatg atcgtccgga cgttggtctt gcggagaacg
     1201 tcgagactgt gccccggcgt ggcgacgacg acgcggccgt caccccagag cctggtccac
     1261 acgaccgggg aggtgatcgg gcggtgccac gggtggtacg gctgcaccgg gtgcgtcgtg
     1321 gtcgccaaca cgtcgatgag atcgtcgtgc agcacccagt actgctccgt ggtgagggtg
     1381 aagtcttcga taccagccgt gatctcgtgc gcgcggccga gctccgtgac gtgcacggtg
     1441 tgggtcagga agttgtccgt ctcgtcgccc acgcgctgcg ccgggtcctt cgacggatgc
     1501 gtcgcgaact gcccaccaat cagctgcagg tagtccgagt tggcgcggta ggagtcggcg
     1561 atcccgccgt gccaccccac cagacccgtg ccccggcgga ccgccgcggc gagccccacg
     1621 aacgcctgat ccgagatctc cgacatggtc acgctctgca cgaccagatc ggtgcgcgcc
     1681 atgagcgcct cgtcggcgta agtctcgttg gagtcctcga tctggacgtc gaacccgtgc
     1741 tcctcgagga acggcacgaa gagattcgtc gcgtcgacgg gactgtgccc gtaccaacca
     1801 ccacgaacga tgagtgcggt gcgagctgtc atcaggggct tccttcaact gtggggtgag
     1861 gtgaggtagg tgcgcccggc gggatcttgc gcacgacgct gtcgccggcc gtggccgacg
     1921 aaacctcgcg ggcgagcgct tcgttcgtca cgtcgacggc cggtcgaggt gcgccacctc
     1981 gctcacctcg ggcacgcctc ggtcgtcgca gcgtgcgtgc c
//
LOCUS       DASRKH010000410         2623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_11915, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000410 DASRKH010000000
VERSION     DASRKH010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11915"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1324
                     /locus_tag="VFC06_06610"
     CDS             <1..1324
                     /locus_tag="VFC06_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZL81589.1"
                     /translation="MKRIVLTTMGMAASVALALAACSSGDSGTSTPSTDSSTPDTSPV
                     TITFTWWGNDDRAARYTEALAVFKEQYPYITVQTSFAPFADYWTARSTEAAGRALPDV
                     MQFDSSYLREYAANGHLLDYTPYIESGTIDLANFDQELVGAGALDGEQVGIAMSTNTL
                     AMFVSPDVAAAAGVEFPAEGYTWEDLNRFIEDVSAADIKTAEGYKIYGSGDYVTTFWF
                     FLQWLVQNDQQPFNDDGALNFDQDDIVEWLTLSADLRDGDLVYPINRGLSLAPLGGFT
                     VNEVVSESSWDNFLAGYSADSGKTNLEMVPIPSGDDGETHNFFRPSMLVASGANTDHP
                     EAAATLVDFLLTDPEVGRIFGTSKGVPADSAQRAAIVTEEGSIDAKVIAYEEAIAETE
                     TSQAPVPVKGFGTIEAKWKALGEELSFKNITPEEFAQQWWDEAEMAIS"
     gene            1381..2325
                     /locus_tag="VFC06_06615"
     CDS             1381..2325
                     /locus_tag="VFC06_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020015891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HZL81590.1"
                     /translation="MDEATGTQKVSAAPRSALKRRRAAEARAGYAFLTPWLLGFFLLT
                     AGPMIASLYLSFTDYNLFSPPEWIGLKNYTMLWNDPQYRKAWQVTGTYVLFGTPTKLI
                     AALAVAMLLNNTRRGQGLYRSVFYAPSLIGASVSIAIVWKAMFIDKGIVDQVGQIFGA
                     AGGGWVGDPKRTMPMLVLLTVWQFGAPMIIFLAGLKQVPNELYEAAAVDGAGPVRKFI
                     KITLPMLSPVIFFNVLLETIHAFQIFASAFIISNGTGGPARSTLFYTLYLYFRGFVDF
                     RMGYASAMAWILVLVVGLITFVFFKTSKSWVHYSGDSK"
     gene            2331..>2623
                     /locus_tag="VFC06_06620"
     CDS             2331..>2623
                     /locus_tag="VFC06_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HZL81591.1"
                     /translation="MSAPVDAGTATYRSDESAARSEVLRKKHRTSERWKSLGFHVLVL
                     AIAVVVLYPAAWMLMSSFKPSSEIVGSVSLVPTNGSFDNFTKALSGIGGVSFWR"
BASE COUNT          441 a          898 c          825 g          459 t
ORIGIN      
        1 tatgaagcgg atcgttctca ccaccatggg catggcggcc tcggtcgctc tggcccttgc
       61 agcctgcagc agcggggact ccgggacgag cacaccctcg accgactcct cgacacccga
      121 cacctctccc gtcaccatca cgttcacgtg gtggggcaac gacgaccgag cggctcgcta
      181 caccgaggcg ctcgcggtct tcaaggagca gtacccctac atcacggtgc agacgagctt
      241 tgcgccgttc gccgactact ggaccgcgcg ctccaccgag gcagctgggc gcgcacttcc
      301 ggacgtcatg cagttcgact cgtcgtacct gcgcgagtac gcggccaacg gccacctgct
      361 cgactacacg ccgtacatcg agtccggcac gatcgacctg gcgaacttcg accaggagct
      421 ggtcggcgcg ggcgcgctcg acggcgagca ggtcggcatc gcgatgtcga ccaacacgct
      481 cgcgatgttc gtcagcccgg acgtcgcggc cgcggccggc gtcgagttcc ccgcggaggg
      541 ctacacctgg gaggacctca acaggttcat cgaggacgtc tccgcggcgg acatcaagac
      601 cgccgagggc tacaagatct acgggagtgg cgactacgtc acgaccttct ggttcttcct
      661 ccagtggctc gtgcagaacg accagcagcc gttcaacgat gacggcgcgc tgaacttcga
      721 ccaggacgac atcgtcgagt ggctcacgct ctcggcggac ctgcgcgacg gcgacctcgt
      781 ctacccgatc aaccgcgggc tctcgctcgc gccgctcggt gggttcaccg tcaacgaggt
      841 cgtgtccgag tcgagctggg acaacttcct ggccggctac agcgccgact ccggcaagac
      901 caacctcgag atggtgccga ttccgtcggg agacgacggc gagacgcaca acttcttccg
      961 gccgtcgatg ctcgtggcct cgggcgcgaa caccgaccac ccggaagcag cagcgacgct
     1021 ggttgacttc ctcctcacgg accccgaggt cggccggatc ttcggcacct ccaagggtgt
     1081 tccggcggac tcggcgcagc gcgccgcgat cgtcaccgag gagggcagca tcgacgccaa
     1141 ggtcatcgcc tacgaagagg cgatcgccga gaccgagacc tcgcaggcgc ccgtgcccgt
     1201 caagggattc gggaccatcg aggccaagtg gaaggcgctg ggcgaggagc tgtcgttcaa
     1261 gaacatcacg ccggaagagt tcgcccagca gtggtgggac gaagccgaga tggctatcag
     1321 ttgaggcagc aggagcccct tcctgcttac gcacgaccgg aacgagacac cgtgaccctc
     1381 atcgacgagg ccaccggtac ccagaaggtc tcggccgccc cgcgctccgc tctcaagcgg
     1441 agacgggcgg ccgaggcccg ggccgggtac gccttcctca ccccgtggct gctcgggttc
     1501 ttcctgctca ccgcaggtcc catgatcgcc tccctctacc tgtccttcac ggactacaac
     1561 ctcttctcgc cgcccgagtg gatcggcctg aagaactaca cgatgctctg gaacgacccg
     1621 cagtaccgca aggcgtggca ggtcaccggc acgtacgtgc tgttcgggac ccccacgaag
     1681 ctcatcgcag cgctcgcggt cgcgatgctc ctgaacaaca cccggcgcgg acagggcctc
     1741 tatcggtccg tgttctacgc tccctcgctc atcggtgcgt ccgtgtcgat cgccatcgtc
     1801 tggaaggcga tgttcatcga caagggcatc gtcgaccagg tcggccagat cttcggcgcc
     1861 gcgggcggcg gctgggtcgg tgacccgaag cgcaccatgc cgatgctcgt cctgctgacc
     1921 gtctggcagt tcggtgcccc gatgatcatc ttcctggctg gcctcaagca ggtgccgaac
     1981 gagctgtacg aggccgcagc ggtcgacggt gcaggtccgg tgcgcaagtt catcaagatc
     2041 acgttgccga tgctctcgcc cgtgatcttc ttcaacgtgc tgctcgagac catccacgcg
     2101 ttccagatct tcgcctcggc gttcatcatc tcgaacggga ccggtggtcc ggcgaggtcg
     2161 acgctcttct acaccctgta tctgtatttc cgcggcttcg tggacttccg catgggctac
     2221 gcctcggcga tggcctggat cctcgtgctg gtcgtcgggc tcatcacctt cgtgttcttc
     2281 aagacctcga agtcgtgggt ccactactcg ggagattcga aatgagcctg gtgagtgcgc
     2341 ccgtcgacgc cgggacggcg acctaccggt cggacgagag cgccgcccgc tcggaggtgc
     2401 tccgcaagaa gcaccgcacg agcgagcggt ggaagtcgct cggcttccac gtcctggtcc
     2461 tggccatcgc ggtggtggtc ctgtatcccg cggcgtggat gctcatgtcg tcgttcaagc
     2521 cgtcgagcga gatcgtcggc tcggtgagcc tggtccccac caacgggtcg ttcgacaact
     2581 tcacgaaggc gctctcgggc atcggcgggg tgtcgttctg gcg
//
LOCUS       DASRKH010000411         2399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_22899, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000411 DASRKH010000000
VERSION     DASRKH010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22899"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2259
                     /locus_tag="VFC06_06625"
     CDS             <1..2259
                     /locus_tag="VFC06_06625"
                     /inference="COORDINATES: protein motif:HMM:NF012731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HZL81592.1"
                     /translation="VRRHVRHRDAGIYDGVVTMTHVPSDGPPIRKLVPLVYGQGGWGD
                     IDNGASQPRYTEARLNDESMLLLGLSESVLGAGEAAEVAENGVRLVKNYSGELDEPTV
                     MPALWPQFVINGVEGIGAGVSTFTPGHHPVEALDLALSLVDTDNPRWATIQKLMPGPD
                     LPADCDIYDIDGGIESYMTTGKGQFMQRARYEVIPTEGKGNKGSTMVVTGFPFRVSPA
                     MVIEGINDQIASEDLPPMSMRNLSDGDGFRLEIDLGNGDTEAIIQRLLYLGRKVHLQE
                     TFSVNSWAIVDNQVRRVSTVDALRHWVAHRRTVIRRRTKFRLDKAEERHEIVVGLLKA
                     VPIAHLIVEVVRSSANRAEAAVEMTKQWGFTERQNQAILDMNVGQLARIGVERYENEK
                     IYLEARITDCKEILGDPKALSARLKDEIRATKKHFAGHERRCHLMEGCAKVERPKVAV
                     SMVPSKKIYIAHTSTWYLRQVAKRNINPIVGADHVVGFLETTSHDMVDVVTNKGNLTR
                     IKADSVPDKATRADALVKVEVGERAILAGSTAGGADVILVTRSGLVKRAGHDDNSAMR
                     PGRPYCVQPLSDGDEVAWAFHARPGADERLIGITANGYVLAVNADDPVFRARSRTARG
                     NSLINVVSGDSLVWVGLVGAKDRVVAWTEAGGTFALKSSQMNTLNRGTKGTRVCHHKS
                     LVAGATSGPGRTLAMFNIGDEGDAAREVDVRTLTEGPEVKRPTVVDGITKAGTVIWLT
                     EAGSLADVDEAA"
BASE COUNT          460 a          790 c          766 g          383 t
ORIGIN      
        1 gtcagacggc acgtgcgtca tcgtgacgcc ggtatctacg acggcgtcgt cacgatgacg
       61 cacgtgccgt ctgacggtcc gccgatccgc aagttggtgc cgctggtcta cgggcagggc
      121 ggttggggcg acatcgacaa cggtgcttcc cagccgcgtt acacggaggc gcgactgaac
      181 gacgagtcca tgctgctgct cgggctgtcc gagtccgtgc tgggcgccgg agaggcagcc
      241 gaggtcgccg agaacggcgt ccgcctggtg aagaactact caggcgaact ggacgagccg
      301 accgtgatgc ccgcgttgtg gccgcagttc gtcatcaacg gcgtggaagg catcggtgcg
      361 ggcgtctcca cgttcacccc cggccaccac cctgtagagg cactcgactt ggcgttgtcg
      421 ctcgtggata cggacaaccc gcgctgggcg accattcaga agttgatgcc gggccccgac
      481 ctgcccgccg actgcgacat ctacgacatc gacggcggca ttgagtccta catgacgacc
      541 ggcaagggcc agttcatgca gcgcgcccgc tacgaggtca tccccacgga gggcaagggc
      601 aacaagggca gcaccatggt tgtcaccggc ttcccgttcc gcgtctctcc cgccatggtc
      661 atcgagggca tcaacgacca gatcgcgtcc gaagacctcc ctccgatgtc gatgcgcaac
      721 ctctccgacg gtgacggctt ccgcctcgaa attgacctcg gcaacggcga cactgaggcg
      781 atcattcagc ggctgctgta cctgggacgc aaggtgcacc tccaagagac cttctcggtc
      841 aactcgtggg cgatcgtcga caaccaggtc cgtcgcgtgt ccaccgtcga cgcgctgcgt
      901 cactgggtgg ctcaccgccg cacggtgatc cgtcgccgca caaagttccg tctcgacaag
      961 gccgaggagc gccacgagat cgtcgtcggc ctgctcaagg cagtgccgat cgctcacctc
     1021 atcgttgagg tcgtccggtc cagcgcgaac cgcgccgaag cggccgtcga gatgactaag
     1081 cagtggggct tcacggagcg ccagaaccag gccatcctcg acatgaacgt cgggcaactg
     1141 gcccgcatcg gcgtggagcg ctacgagaac gagaagatct acctcgaagc acgcatcacc
     1201 gactgcaagg aaatcctcgg agaccccaag gcgctgtctg cccgcctcaa ggacgagatc
     1261 cgtgcgacga agaagcactt cgcgggccac gagcgacgct gtcacctgat ggagggctgc
     1321 gccaaggtgg aacgccccaa ggtcgcagtg agcatggtgc cgtccaagaa gatctacatc
     1381 gcgcacacgt ccacctggta tttgcgccag gtcgccaagc gcaacatcaa ccccatcgtg
     1441 ggtgctgacc acgtcgtcgg gttcttggag accacgagcc acgacatggt cgacgtcgtc
     1501 accaacaagg gcaacctgac tcgcatcaag gctgactcag ttcccgacaa ggcgacccgc
     1561 gctgatgcgc tggtcaaggt cgaggtgggg gagcgagcaa tcctggcggg gtcaaccgcc
     1621 ggcggcgctg acgtcatcct ggtgacccgc agcggccttg tgaagcgagc cggtcacgac
     1681 gacaactcgg cgatgcgccc agggcgcccg tactgcgtcc agccactgtc tgatggcgac
     1741 gaggtcgcct gggccttcca cgcccgtcct ggggccgatg agaggctcat cgggatcaca
     1801 gcgaacgggt acgtgctggc cgtgaacgcc gacgaccctg tgttccgtgc gaggtcacgt
     1861 actgcgcgag gtaactcgct catcaacgtg gtcagcggtg actcactcgt gtgggttgga
     1921 ctggtaggcg ccaaggatcg cgtcgtcgcc tggaccgaag ccggcggcac cttcgcgctc
     1981 aaatcttcgc agatgaacac gctcaaccgc ggtacgaagg gcacccgggt gtgccaccac
     2041 aagtcgctgg tcgccggagc cacatccggc cccggccgca cgttggccat gttcaacatc
     2101 ggagatgaag gcgatgcagc acgcgaagtc gacgtgcgga cgctcaccga agggcccgag
     2161 gtcaagcgcc ccactgtcgt cgacgggatc accaaggctg gcaccgtcat ctggctgaca
     2221 gaagcaggca gccttgccga tgttgacgag gccgcctgac gaagacggcg agacgtactc
     2281 gctagacgaa gcgcacctga cgctggtcct ggccctctac ccgaacccgt agggccgccg
     2341 aactacttgg ctgcggcatc tgctggcgtc gctgcatcac gagcgatacg gcggatctc
//
LOCUS       DASRKH010000412         2752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_42500, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000412 DASRKH010000000
VERSION     DASRKH010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42500"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1056)
                     /locus_tag="VFC06_06630"
     CDS             complement(<1..1056)
                     /locus_tag="VFC06_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HZL81593.1"
                     /translation="MRTAEISKRWLEYFESKGHSVVPSTSLVSPDPSLLFTVAGMVPF
                     IPYIIGSETSPHPRIASVQKCIRTKDIEEVGKTTRHGTFFQMLGNFSFGDYFKEGAIE
                     FAWDFLTGPESEGKLGFEPDRFWVTIWEKDDDSFTYLKNVGVNPDHIVRLKREEIFWD
                     TGQPGPAGPCAEWHYDRGLAYGPDAVGGTVDPGGDRYLEIWNLVFDQFLRGPGSGKDY
                     PLIRELEQKAIDTGAGLERIAVLKQGVENFFEIDEVFPVIAKAQELSGRVYGKDVDDD
                     IRFRVIADHVRSSLMLIGDGVTPGNEGRGYVLRRLLRRSVRSMRLMGVNGPSLVALIE
                     ASYEAMRESYPELDKDFD"
     gene            complement(1150..1776)
                     /gene="rpsD"
                     /locus_tag="VFC06_06635"
     CDS             complement(1150..1776)
                     /gene="rpsD"
                     /locus_tag="VFC06_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HZL81594.1"
                     /translation="MTQVQRARRQVRLSRALGIALTPKAVKHFEKRPYPPGEHGRTAR
                     RKESDYAVRLREKQRLRAQYGLREKQMARTFEEARKEAGLTGEAFVELLEMRLDALVL
                     RSGFARTILQARQTVLHRHILVDGKIVDRPSFRVSPGQTIQVKAKAVTMEPYMAAAAG
                     AHRDLLPEVPDYLDVQLEKLTAQLLRRPKRAEVPIICEVQLVVEYYAR"
     gene            complement(1901..>2752)
                     /locus_tag="VFC06_06640"
     CDS             complement(1901..>2752)
                     /locus_tag="VFC06_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HZL81595.1"
                     /translation="SSSRATSPPKPLTGVDIGALLDRAVADPRGLGGEVALDDDARDH
                     MIRVAGADARKALTLLESVADAAADNDGVITAAVTEAAMDAALVAYDKSGDQHYDVIS
                     AFIKSVRGSDVDAALHYLARMVVAGEDPRFIARRLVILASEDVGLADPQALVIAQAAA
                     DAVSFIGMPEGRIPLGEATAYLAMAPKSNRSYLAIDAAIADVRAGRAGVVPTHLRDAH
                     YAGAERIGHGKGYEYSHDAAHGVAAQEYLPASLAGTRYYRPTEHGNERSLSDRLATLR
                     KLLGREN"
BASE COUNT          473 a          865 c          929 g          485 t
ORIGIN      
        1 gtcaaagtcc ttgtccaact cgggatacga ctcccgcatg gcctcataac tcgcctcgat
       61 gagtgccacg agcgaggggc cgttgacgcc catcaggcgc atcgagcgga cggacctgcg
      121 cagcaggcgg cgtagcacgt agccgcgacc ctcgttgccg ggagtcacgc cgtcgccgat
      181 cagcatgagc gaggagcgca cgtgatccgc gatcacgcgg aagcggatgt cgtcgtcgac
      241 atcctttccg tagacccggc ccgacagctc ctgagccttc gcgatgaccg ggaagacctc
      301 atcgatctcg aagaagttct cgacgccttg cttgaggacc gcgatgcgtt ccaggcccgc
      361 gccggtgtcg atcgccttct gctcgagttc gcggatcagg gggtagtcct tgcccgaacc
      421 cgggccgcgc aggaactggt cgaacaccag attccagatc tcgaggtagc ggtcgccgcc
      481 agggtcaacg gtgccgccca cagcgtcagg cccgtatgcc agcccgcggt cgtaatgcca
      541 ctcggcacag ggaccggccg gtccgggctg gccggtgtcc cagaagatct cttcgcgctt
      601 caggcgcacg atgtggtcgg gattgacgcc cacgttcttg agatacgtga aggagtcgtc
      661 gtctttttcc cagatggtga cccagaagcg gtcgggttcg aagcccaact taccttcgga
      721 ctcgggtccg gtgaggaagt cccacgcgaa ctcaatcgcg ccttccttga agtagtcgcc
      781 gaacgagaaa ttgcccagca tctgaaagaa cgtgccgtgc cgggtcgtct tgccgacttc
      841 ttcgatgtcc ttcgtgcgga tgcacttctg caccgaggcg atgcgggggt gcggcgacgt
      901 ttctgacccg atgatgtatg ggatgaacgg caccatgcca gccaccgtga acaggagcga
      961 cgggtcagga gacaccaacg atgtcgaggg cacaactgag tgacccttgg actcgaagta
     1021 ctcaagccag cgcttgctga tctcagcggt tcgcatcact acctccacac gagacaacgc
     1081 cgccgggcca cgacttgtgc catgggcccg gcggacgttg gttcaggtct gcaaagagac
     1141 tagtgcgtct taacgcgcgt agtactcgac gaccaactgc acttcgcaga tgatcggaac
     1201 ttcggcacgc ttgggccgac gcagcagctg ggccgtcagc ttctcgagct gaacgtccag
     1261 gtagtcgggc acctcaggca gcaggtcgcg gtgtgcgccg gctgcggcgg ccatataggg
     1321 ctccatggtg acggccttgg ccttgacctg gatggtctgg ccgggtgaca cgcggaacga
     1381 cggacggtcg acgatcttgc cgtcgacgag gatgtggcgg tgcagaaccg tctggcgcgc
     1441 ctgcaggatg gtgcgggcga atccggagcg cagcacgagc gcgtcgaggc gcatctcgag
     1501 caactcgacg aaggcctcac cggtcagacc ggcttccttg cgagcctctt cgaacgtgcg
     1561 cgccatctgc ttctcgcgca gaccgtactg ggcgcgaaga cgctgcttct cgcgtaagcg
     1621 gaccgcgtag tcgctttcct tgcgacgcgc ggtacgaccg tgctcgccgg gagggtaggg
     1681 gcgcttctcg aagtgcttga cggccttggg ggtgagcgca atgccgagag cgcgggacag
     1741 acgcacctgg cggcgcgcgc gttgaacctg agtcattact ttccttaagt gggaggcgtg
     1801 tcaagacacg caaaagccgt tcactatctg tgatgtcgcg gcgacagcag ccaaccgtcc
     1861 gcgagggcag acgttcatga tagcaagaaa cgagggaaag tcagttctct cggcccagga
     1921 gtttgcggag cgtcgcgagc ctgtccgaga gcgaccgctc attgccgtgt tcggtgggtc
     1981 ggtagtaccg ggtcccggcg agcgatgcgg gcaaatattc ttgcgcggct acgccgtgtg
     2041 cggcgtcgtg cgagtactcg tagcccttgc cgtggccgat gcgctcggca cctgcgtagt
     2101 gcgcgtccct gaggtgggtt ggcacgactc ctgcacgccc ggcccggacg tcggcgatgg
     2161 ccgcgtcgat ggcaaggtag gagcggttgg acttgggcgc catcgccagg tacgcggtcg
     2221 cttctcccaa ggggatgcgg ccttcgggca tgccgatgaa gctcactgcg tcggccgcgg
     2281 cctgggcgat gacgagcgcc tgggggtcgg cgaggccgac gtcttcggag gcgaggatca
     2341 ccagcctccg cgcgatgaaa cgggggtcct cgcccgcgac caccatgcgt gccaggtagt
     2401 gcagggcggc gtcgacgtcc gagcccctga ccgacttgat gaacgcgctg atgacgtcgt
     2461 agtgctggtc gcccgacttg tcgtacgcga cgagcgcggc gtccatggcc gcctccgtca
     2521 ccgcagctgt gatgacgccg tcattgtctg cggccgcatc cgcgaccgac tccagcaagg
     2581 tgagcgcctt gcgcgcatcg gcgcccgcca cccggatcat gtggtcgcgg gcgtcgtcgt
     2641 cgagagcgac ctcgcctccc aagccgcgcg gatccgcgac ggcccggtcg agcaacgcgc
     2701 cgatgtccac gcccgtgagc ggcttgggag gcgaggtcgc tctcgacgac ga
//
LOCUS       DASRKH010000413         2285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_11772, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000413 DASRKH010000000
VERSION     DASRKH010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11772"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            366..>2285
                     /locus_tag="VFC06_06645"
     CDS             366..>2285
                     /locus_tag="VFC06_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HZL81596.1"
                     /translation="MHVTKRDGTREPYNADRINKAIERAASGLPDQISITTQVASELA
                     ITLFDGITTEQLDEAAIGVAVQNVKDDPDFDIVASRLLRKVIYKRVLGGFDTDLELAL
                     LHQARFPGYIRDAVEEGLLDTRLELLFDLDALAAAIDPRRDDLMRYIGTVTMRNRYMI
                     TDRHGKALEVPQYFWMRVSMGLSLNEANPTEMAVKFYEKISRLEYLPAGSTLVNAGTS
                     YPQLSNCFVMQMEDDMDHIAKTVGDVMWLTKGTGGIGLSVTKLRSEGSPIKSNNTTST
                     GPIPFMHTIDSTLRAVSRGGKKFGALCFYMENWHLDFPQFLDLRQNSGDPYRRTRTAN
                     TAVWISDEFMKRVAANEDWYLFDPADAPDLVELVGSAFSRRYKELVALAEAGKIRRFR
                     KMRAREQYKAILISLQASSHPWLTWKDTINNRALNTNTGAIHLSNLCTEICLPQDREN
                     IAVCNLASINLSAHFIEGKIDWERMETSVRLSVRQLDNLVDITLSSVPESDHANKENR
                     AIGLGVMGFTDVTEKMGLAYESEESYDLIDQIVEFVSYHAIDESADLARERGSYANFE
                     GSGWSKGLVPFDTIAIAEADREVPIAVNRTMRQDWDTLRAKVAGGMRNATLMAVAPTA
                     SIGLVAGTTPGFDPQF"
BASE COUNT          441 a          772 c          674 g          398 t
ORIGIN      
        1 ttgaacaggg tgggactgca agagtggaag acacgccgcg ttgtccacag cttatgcaca
       61 ggtccctggg gatgaccact acatctaggg ttatttgtgt cccgacaccc ctacatcttg
      121 tatttttctg ttattgtcgg atccagcgct cgggccggtc gtgggtggcc acgaccccca
      181 tgaccagccc gcgccaatgc ctcgcacagc gtgttttacc cggcgacaca ccgtcggaat
      241 agcaatcggg ccttgtggcc ttccgtcccc gaaggagcat cgtgacgatc actgagaaca
      301 cggcaactga cgtcaacggc gacgtcgctt ccgcagctgc cgaagtcaac ccgagccgct
      361 cgggtatcca cgtcaccaag cgcgacggca cacgcgagcc gtacaacgcg gatcggatca
      421 acaaggcgat cgagcgggcc gcctccggtc ttcccgacca gatctccatc accactcagg
      481 tggcctccga actggcgatc acactgttcg acggcatcac caccgagcag ttggacgagg
      541 ccgcgatcgg cgtggccgtc cagaacgtca aggacgaccc cgacttcgac atcgtcgcct
      601 cccgcttgct acgcaaggtg atctacaagc gcgtgctcgg cggtttcgac accgacctgg
      661 agctcgcgct gcttcaccag gcgcgcttcc ccggctacat tcgcgacgcg gtcgaggagg
      721 gcctgctcga cacacgcctc gagttgctct ttgatctcga cgccctcgcg gcggccatcg
      781 acccccgtcg cgatgacctg atgcggtaca tcggcactgt cacgatgcgc aaccgctaca
      841 tgattaccga ccgtcacggc aaggcgcttg aggtgccgca gtacttctgg atgcgcgtgt
      901 cgatgggact gtcgctcaac gaggccaatc ccaccgagat ggcggtcaag ttctacgaga
      961 agatctcgcg cctcgagtac ctgcccgccg gctcgacgct cgtgaacgcg ggaacgtcat
     1021 acccgcaact gtccaattgc ttcgtgatgc agatggaaga cgacatggac cacatcgcca
     1081 agaccgtcgg cgacgtcatg tggctcacca agggcacggg cggcatcggc ctctcggtga
     1141 ccaagctgcg ctccgagggc agccccatca agtcgaacaa caccacgtcg accgggccca
     1201 tcccgttcat gcacacgatc gactccacgc tgcgcgccgt gtcgcgtggg ggcaagaagt
     1261 tcggcgccct gtgcttctac atggagaact ggcacctcga cttcccgcag ttcctcgacc
     1321 tgcgccagaa ctccggagac ccctaccgcc gcacgcgtac ggcgaacacg gccgtgtgga
     1381 tctccgatga gttcatgaag cgcgtggcag cgaacgaaga ctggtacctc ttcgaccccg
     1441 ccgacgctcc cgacttggtc gaactcgtcg ggtcggcatt ctcgcgccgc tacaaggagt
     1501 tggtggcgct tgcggaggct ggcaagatcc gcaggttcag gaagatgcga gcccgcgagc
     1561 agtacaaggc gatcctcatc tcgctgcagg cgtcgtcgca cccgtggctc acgtggaagg
     1621 acacgatcaa caaccgtgcc ctcaacacca acacgggcgc catccacctg tcgaacctct
     1681 gcacggaaat ctgcctgccg caggaccgcg agaacatcgc ggtctgcaac ctcgcatcga
     1741 tcaacctgtc cgcgcacttc atcgagggca agatcgactg ggagcgcatg gagacctcgg
     1801 tgcgcctgtc ggtgcgccaa ctcgacaacc tcgttgacat cacgctctcc tccgtccccg
     1861 agtcggatca tgccaacaag gagaaccgcg cgatcggcct gggcgtcatg ggcttcaccg
     1921 acgtgaccga gaagatgggt ctcgcctacg agtccgaaga gtcgtacgac ctcattgacc
     1981 agatcgtcga gttcgtgtcc tatcacgcga tcgacgagtc ggccgacctt gcgcgtgagc
     2041 gcggctcgta tgcgaacttc gagggctccg gttggtccaa gggcctcgtg cccttcgaca
     2101 ccatcgcgat cgccgaggcg gaccgtgagg tgccgatcgc cgtgaaccgc accatgcgac
     2161 aggactggga caccctgcgt gcgaaggtcg cgggcggaat gcgcaatgcg acgctgatgg
     2221 ccgtcgcgcc gacggcttcg atcggcctcg tcgcaggcac cacgccgggc tttgacccgc
     2281 agttc
//
LOCUS       DASRKH010000414         4030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_22527, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000414 DASRKH010000000
VERSION     DASRKH010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22527"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..858)
                     /locus_tag="VFC06_06650"
                     /pseudo
     CDS             complement(1..858)
                     /locus_tag="VFC06_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268613.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
     gene            complement(860..1378)
                     /locus_tag="VFC06_06655"
                     /pseudo
     CDS             complement(860..1378)
                     /locus_tag="VFC06_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002549347.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
     gene            complement(1450..2433)
                     /gene="arcC"
                     /locus_tag="VFC06_06660"
     CDS             complement(1450..2433)
                     /gene="arcC"
                     /locus_tag="VFC06_06660"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HZL81597.1"
                     /translation="MRIVIALGGNALLERGQKPDARPQQANVDRAVAALAPLADHHEI
                     VLTHGNGPQVGVLALQSANDPNLSEPYPFDTLGAMTQGMIGYWMLQSLQNHLPGRHVA
                     SVINQTLVLSGDPAFDNPTKFVGQVYNEEEAARLTEERGWVMKPDGQFFRRVVGSPKP
                     QRVVETRIIRTLIEAGAVVVCGGGGGIPVIRDERGLRKGVEAVIDKDLTAAVLAEALE
                     ADFLMILTDVPAVLDHYGTPEQVEINRATPSFMRKRGFAAGSMGPKVEAACRFVELTG
                     DTAAIGRLEEAEMIVAGAAGTIITPGGNYGGPDDIRPPLPEPVESRKVRRA"
     gene            complement(2438..3439)
                     /gene="argF"
                     /locus_tag="VFC06_06665"
     CDS             complement(2438..3439)
                     /gene="argF"
                     /locus_tag="VFC06_06665"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HZL81598.1"
                     /translation="MSIDLKGRSFLKELDFTPEEWRHLLDLSAELKAAKKEGREVQHL
                     AGKNIALIFEKTSTRTRCSFEVAAFDQGAHITYLDPSGSQMGHKESVADTARVLGRYY
                     DGIEFRGSKQEHVETLAALSGVPVWNGLTDDWHPTQMLCDLLTMHEQSGKDYKEISFA
                     YVGDARNNVANSLLISGAMMGMDVRMVGPAALHNDPAIVAEAERIAAETGAKVTVTDD
                     PRAGVAGCDFIYTDVWVSMGEPKEVWDERIALLSPYQVNAELMAATGNADAKFLHCLP
                     AFHDRNTKVGEDIFQKTGMESLEVTDEVFESPASVVFDQAENRMHTIKAVMVATLGA"
     gene            complement(3493..>4030)
                     /locus_tag="VFC06_06670"
     CDS             complement(3493..>4030)
                     /locus_tag="VFC06_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HZL81599.1"
                     /translation="IGLSERTTAQGVERLARRLFEKEAAETIIALHMPAQRAMMHLDT
                     VMTMVDETSFTAYQGLGNLPSVTIRPGPSSGKLSLTRNTADGMFAVIGDALGIGTPRV
                     LITPQDAIAAEREQWDDGCNLLAVRPGVVVGYERNVTSNNYLRSQGIEVIEVPGSELG
                     RGRGGPRCMSCPTQRDPI"
BASE COUNT          669 a         1270 c         1349 g          742 t
ORIGIN      
        1 aataaacagc agcggacgtc agcaacccga cgagccatga catgtccact ccacccattg
       61 cggtggcaat cggaccctgt gcggcgggtg cggatccgta cgtgaacaac cacgtcatga
      121 acatgccgac gaaaaacgcc acgtaacccg ccgagttgac aacgggcaat ctcttcgttc
      181 cgactgcgtc gagcagcacc gcgaagtcgg agcgtttgcg ttcgattcgg tagaagtgga
      241 cgagcatgat tgccggccca cgtcgacacc catgcgacca gggtcaccag ccatgtgtcg
      301 aggaccgagg cgaagtcatc ctggaagatc aggaccacga caaccagcat tgagaagacc
      361 ccgaccactg tgctgagttt gcgccgaccg atcgaagtgt caaacgcctg tgttgccacc
      421 gaacgtgtag atgttgagaa tattcgtcgc gctcggacca tgaaccacaa gcatcagaac
      481 cggaatggcg aggctggcga agttctccac gatgagttgt ccggtggtgg ccaggcttgc
      541 tccgaggaga ccaagccaca ccacaggaat gaactgaccc gctgcgctcg caatgtagag
      601 ctttgtccgt gggacgctcc gcgaaacgaa acgggagtag tccgcggcat aggtgaacca
      661 cgtgatgccc caaccaattc caatcgccgt catcaccgag gtgagcgccg caagtcgagc
      721 aggaccctcg agtacagctc caggcagccc tgcgtaggaa aagtcgaggt ccatgccgaa
      781 ccacgcgacc gcgggcacga tccacgtgtt gaccgcggac cacccgatcg aaagtaccgc
      841 ttgaatgccc gcgggcatgc agcgagatct tctcgtcgta gtagttgtgc ttgtaggact
      901 tgtccgtcgg gcgcggccgg ggcgcctggg tccacttgaa ctccgggtcc tcctcgaaat
      961 actggttcat caggtcccag tacgccaggt actcgaagta gcggcagcgg aacgagttcg
     1021 ccgacgccat gatctcgttg ccgatggtca gcaggatgtc gcggggcggc atctgggtga
     1081 acatcgagtc gttgcggaag tccggcgtct ggatgtgctg gttccactgc aacgacgtcg
     1141 ggcggtcaac cttgatgccc agaccctcga ggatcttcac gtagttgttg agctgctcgt
     1201 tcgccgcctc cacggtcgcg acaggccgcg gaccccacat gccgcgcatc ggggagtcga
     1261 tcggcacctt ctccgaggtg gccggctcct ccggcgggat gacgcagtgg tcagcaagac
     1321 ccacgatcac gtgcttcggg ggatcgaaat cgttccacga attgacgatc ttcgtcatgt
     1381 tcactcctat gtgttgtgtc tcctaggttg gtttcggttg gtcagggacg gcccgctggc
     1441 cgtcgggagt caggcgcggc ggacttttcg gctctcgacc ggttcgggca gcgggggccg
     1501 gatgtcgtcc ggtccgccgt agttcccgcc aggcgtgatg atggtcccgg ccgcgccggc
     1561 cacgatcatt tccgcctcct ccaggcggcc gatggctgcc gtgtcacccg tgagttccac
     1621 gaaccggcag gctgcctcga ccttcggccc catcgagccg gcggcgaagc cgcgcttgcg
     1681 catgaaggac ggggtcgcgc gattgatctc gacctgctcc ggcgtgccgt agtggtcgag
     1741 cacggcggga acgtcggtga ggatcatgag gaagtcggcc tcgagcgcct ccgcgagcac
     1801 ggcggcggtg aggtccttgt cgatgactgc ctcgacgccc ttgcgcaggc cccgctcgtc
     1861 gcgaatcacg gggatcccgc cgccaccacc gcagaccacc acggccccgg cctcgatgag
     1921 ggtccggatg atccgtgtct cgacgacgcg ttgcggcttc ggcgatccga cgacgcgacg
     1981 gaagaactgg ccgtcgggct tcatgaccca accccgctcc tcggtgaggc gcgcggcctc
     2041 ctcctcgttg tagacctgcc cgacgaactt ggtggggttg tcgaacgccg ggtcgcccga
     2101 gaggacgagc gtctggttga tcaccgacgc gacgtggcgc ccgggcaggt ggttctgcag
     2161 cgactggagc atccagtagc cgatcatccc ctgggtcatg gcaccgaggg tgtcgaaggg
     2221 gtagggctcg gagaggttcg ggtcgttcgc cgactgcagg gcgagcacgc ccacctgtgg
     2281 accattgccg tgggtgagca cgatctcgtg gtgatccgcg agcggcgcga gggcggcgac
     2341 ggcccggtcg acgttggcct gctggggccg cgcgtcgggc ttctggcctc gctcaaggag
     2401 tgcgttgccg ccgagtgcga tgacgatgcg catcttctca ggcccccagg gtggcgacca
     2461 ttacggcctt gatcgtgtgc atccggttct cggcctggtc gaagaccacg gaggccggcg
     2521 actcgaagac ctcgtcggtg acctcaaggg attccatccc ggtcttctgg aagatgtcct
     2581 cgcccacctt ggtgttgcgg tcgtggaacg cgggaaggca gtgcaggaac ttggcgtcgg
     2641 cgttgccggt cgcggccatg agctcggcgt tcacctggta cggggagagc agcgcgatgc
     2701 gctcgtccca gacctccttg ggctcaccca tcgagaccca cacgtcggtg tagatgaagt
     2761 cgcagcccgc aactccggcc ctggggtcgt cggtgacggt caccttcgcg ccggtctcgg
     2821 ccgcgatccg ctcggcctcg gcgacgattg ccgggtcgtt gtgcaaggcg gccggaccga
     2881 ccatgcggac gtccatcccc atcatcgcgc cggagatcag cagcgagttc gcgacgttgt
     2941 tccgcgcgtc gccgacgtag gcgaaggaaa tctccttgta gtccttgccc gattgctcgt
     3001 gcatggtcag caggtcgcac agcatctgcg tggggtgcca gtcgtcggtc agtccgttcc
     3061 agaccggcac accggacagc gccgcaaggg tctcgacgtg ctcctgcttc gagccgcgga
     3121 actcgatgcc gtcgtagtac cggccgagca cccgtgccgt gtccgcaacg gattccttgt
     3181 ggcccatctg ggagccgctc gggtccaggt aggtgatgtg ggcgccctga tcaaacgcag
     3241 cgacctcgaa tgagcagcgg gtgcgggtgg aggtcttctc gaagatgagg gcgatgttct
     3301 tgccggcaag atgctggacc tcacgcccct ccttcttggc ggccttcagc tcggctgaca
     3361 ggtcgagcag gtgacgccac tcctcggggg tgaagtcgag ctccttcagg aagctccggc
     3421 ccttcagatc gatgctcatc gattgtcctt tcacgtgtgt gtgcgagttg tccgttgtgc
     3481 gggtgggttg gctcagattg ggtcacgctg cgtcgggcag ctcatgcagc gcggtcctcc
     3541 gcgaccgcgc ccgagctccg agcccgggac ctcgatgacc tcgatgccct gggagcgcag
     3601 gtagttgttg gaggtgacgt tgcgctcgta ccccacgacg acgccggggc gtaccgcgag
     3661 gaggttgcag ccgtcgtccc actgctcgcg ttcggcggcg atggcgtcct ggggtgtgat
     3721 cagcacccgc ggggtcccga tgcccaacgc gtcgccgatc acggcgaaca tcccatccgc
     3781 ggtgttgcgc gtcagggaga gcttgccact cgaggggccg ggacggatgg tgacggacgg
     3841 caggttcccg agcccctggt aggcggtgaa gcttgtctcg tcgaccatgg tcatgacggt
     3901 gtccaggtgc atcatggcgc gctgggcggg catgtgcagc gcgatgatcg tctccgccgc
     3961 ttccttctcg aagaggcggc gcgccaggcg ctccacgccc tgcgctgtcg tgcgctcgct
     4021 gagcccgatg
//
LOCUS       DASRKH010000415         2068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_47447, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000415 DASRKH010000000
VERSION     DASRKH010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47447"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..993
                     /locus_tag="VFC06_06675"
     CDS             178..993
                     /locus_tag="VFC06_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007542646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZL81600.1"
                     /translation="MNDTTNNTRLAGKHVLITGAAQGMGAAVARNYAAQGAKVCLADV
                     NIEGVAQVAQEIRDAGGTATHVKLDVTSEEEAAAAVAHTVKEFGSINVLLNNAGINKP
                     LFFLDVTRENWQRIMDVNAWGTLNCMQAAARQMKEQGKQEDPYKIINVGSILSREAFI
                     DVTVYTASKHAVLGMIKAGAKSLIEHNITVNGYGPGVVRTELWEQLDKDLVEMGMFEK
                     RGESMDSIAENMILMKRYSYPEDIVGTAAFLASPDSDYMTGQLLMIDGGMVIQ"
     gene            1165..>2068
                     /locus_tag="VFC06_06680"
     CDS             1165..>2068
                     /locus_tag="VFC06_06680"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZL81601.1"
                     /translation="MEVLAEVSDATSAEALQAVGAAHDAFSSWSQTAPRQRAELLRRA
                     FEIMTDEIEDCARLIALENGKAWRDAMGEALYAAEFFRWFSEEASRIPGDFRYAPNRD
                     KTIIVDHRPVGVAYMITPWNFPAAMATRKLAPALAAGCTAVLKPAGETPLTALWIADV
                     LQRAGAPAGVVNVITTSEAGEVSSAVLADSRVATLSFTGSTPVGKLLLAQTAQRVLPS
                     SMEMGGNAPCLVLEGSDVDRAVETAMVAKMRNGGSSCIAANRFYVHESLADDFSQRFQ
                     AALAALRIGPGVDQTNDLGAMVSVK"
BASE COUNT          428 a          624 c          646 g          370 t
ORIGIN      
        1 tgcggtgctg gtggacggcg actcccacga gagttgcgga ttcgtgttgt ctcccacgca
       61 ttgaccgccc actcaagttc tgacgaaatt cccggcacaa gaactgtttg tcgccaatga
      121 atactgatca agatgtgctc caccacagca catatctggc aaggaagaga gcccccaatg
      181 aacgacacaa cgaacaacac gcgactcgcg ggcaagcacg tcctcattac cggagccgcc
      241 caaggaatgg gtgccgcggt cgcgaggaac tacgccgccc aaggcgccaa agtgtgcctc
      301 gctgacgtca acatcgaagg cgttgcccaa gttgcgcagg agatccgcga cgcgggcggc
      361 actgccaccc atgtgaagtt ggatgtgacc agcgaagagg aagcagccgc agcggtcgcg
      421 cacaccgtca aggaatttgg cagcatcaac gtgctcctca acaacgcggg catcaacaag
      481 ccactgttct tcctcgacgt cacgcgagag aactggcagc gcatcatgga cgtcaacgcg
      541 tggggaacgc tgaactgcat gcaggctgcc gcgcgtcaga tgaaggaaca gggcaagcag
      601 gaggatccct acaagatcat caatgtcggc tcgatcctgt cccgtgaggc attcatcgat
      661 gtgacggtct acaccgcaag caagcacgca gtgctcggaa tgatcaaggc cggcgcgaag
      721 tcgctcattg agcacaacat cacggtgaac ggctatggac caggcgtggt gcgcaccgaa
      781 ctgtgggagc agctcgacaa ggacctcgtc gagatgggca tgttcgagaa gcggggggag
      841 tcgatggact ccatcgctga gaatatgatc ctcatgaagc gttactctta tcccgaggat
      901 atcgtgggaa ccgcagcctt tttggccagt ccagatagcg actacatgac cggtcagctg
      961 ctcatgatcg atggcggcat ggtcatccag taacgtcgac agcgggtgtc ctgtcgacac
     1021 cgatcggaca cgtgtcgtca tctcttgatc ccagcccagc gaaaggggac tctcatttct
     1081 acttcgaagc accaatcgga tctgctcatc ggcggtgagt ggaccaagac gtcaaagagg
     1141 ttcgaggttc tcaaccccgc ctccatggag gtgttggcgg aggtgagcga tgccacttcc
     1201 gcggaagcac tccaggcggt cggtgccgca cacgacgcgt tctcgtcgtg gtctcagacg
     1261 gccccgcggc aacgggcaga acttctccgc cgcgcgttcg agatcatgac cgatgagatc
     1321 gaggactgtg cgcgcttgat cgcgctggag aacggcaagg cgtggcggga cgccatgggc
     1381 gaggctctct atgcggccga gttcttccgc tggttctcgg aggaggcttc tcgaatcccc
     1441 ggcgacttcc ggtatgcgcc gaaccgcgac aagaccatca tcgttgacca tcgccccgtg
     1501 ggcgtggcct acatgatcac tccctggaac ttcccggcgg cgatggccac ccgcaagctc
     1561 gcgccagccc ttgcggccgg ctgcaccgct gtgctgaagc cagccggaga gaccccgctc
     1621 acggcgctct ggattgccga cgtgctccaa cgtgcgggcg cccccgccgg agtcgtcaac
     1681 gtgattacga catccgaggc gggcgaggtg tcgagcgccg tgttggccga ttcccgcgtg
     1741 gccaccctgt cgttcacggg atcaacgcct gtcggcaaac tcctgctggc gcagacggcc
     1801 cagcgggtat tgccgtcttc gatggagatg ggtggaaacg ctccgtgtct cgtgcttgaa
     1861 ggctcggatg tggaccgggc ggtcgagacc gcgatggtgg caaaaatgcg caacggcggg
     1921 tcgtcatgca ttgcggcgaa tcggttctac gtgcacgagt cacttgccga cgacttctca
     1981 cagcggtttc aggcggcgtt ggcagccttg cgcatcgggc ccggcgtcga tcaaaccaac
     2041 gacctgggcg cgatggtcag cgtcaagg
//
LOCUS       DASRKH010000416         2139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_20507, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000416 DASRKH010000000
VERSION     DASRKH010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20507"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VFC06_06685"
     CDS             <1..325
                     /locus_tag="VFC06_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HZL81602.1"
                     /translation="DIGIFVATRLRDFVHNGSTSLSVNLPNLSLELQQGSHRIVHLHI
                     NTPGVLAAVNQVFAEHGVNIEAQMLATRGELGYVVTDVSAGLTDDAVEALSDMQATVR
                     LRVLS"
     gene            370..>2139
                     /locus_tag="VFC06_06690"
     CDS             370..>2139
                     /locus_tag="VFC06_06690"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZL81603.1"
                     /translation="MLHTLARFELTNAFPIQSATIADALAGRDVLGKARTGSGKTLAF
                     GLPMLARLAGSEKPGPRRPLALILVPTRELAMQVGDALEPYTHAMGLSIRLIAGGLSM
                     SKQIMSLERGVHVLVATPGRLSDLMRRGNADLSQVRITVLDEADHMAEMGFAEEITEI
                     LDKTSPDAQRLLFSATLDGVVDTLVARFMTNPVMHDVTDGNAAPSQRHVVLSVPPHEK
                     YRMATRIAAREGRTLCFVRTKLAADRVAADMRSQGVLAVALHGDRAQNERNLAMTEFR
                     AGRLPVLVATDVAARGIHVDAVDLVLQIDPPADYRDYTHRAGRTGRAGADGLAVLLSL
                     PHQRNAVQRILENANITAEDESFRTIDAMALQRIDQLTGAREPTGGWVDEPLPPARNE
                     RRGSRGRPDRQGGRGDRQGGRVERRPSRPSGRRDESSAGRDQMTARERELAERERSLA
                     ERERRLAQAEASQSAPSAAPASARSDERRSAPPRKTFDRDDKRPPRKTFDRDDKRSTR
                     DDARPSYDDRRPTRDDKHPARDDARPAWKDSRPPRDDRGGARGGSAPRWADKGARDDT
                     RAPRDDSRGDRGERPAYRGKPDSA"
BASE COUNT          352 a          784 c          689 g          314 t
ORIGIN      
        1 cgacatcggc atctttgtcg ccactcggct gcgggacttc gtccacaacg gctccacgtc
       61 gctgtccgtc aaccttccca acctgtcgct cgaattgcag cagggctccc accggatcgt
      121 gcacctgcac atcaacacgc ctggcgtgct cgccgccgtg aaccaagtat tcgccgagca
      181 cggcgtgaac attgaggcac aaatgctggc cactagagga gaactgggtt acgtggtgac
      241 cgacgtctcg gctggactca cggacgacgc agtcgaagcc ctgtccgaca tgcaggcgac
      301 tgttcgcctg cgggtgctgt cataaacgcg gccggggtac ctttcgcaga cctgggcctg
      361 cccgacgagt tgctgcacac gctcgctcgc tttgaactga ccaacgcgtt ccccatccag
      421 tcggcgacga tcgccgatgc cctggccggg cgtgacgtgt tgggcaaggc ccgcacgggt
      481 tccggcaaga cgctcgcgtt tggcctgccg atgctcgccc ggttggcggg ttccgagaag
      541 cccggaccgc gtcgtcccct cgcgctgatc ctggtcccca cccgcgaact cgcgatgcag
      601 gtgggcgacg cccttgagcc gtacacccac gccatgggcc tgtcgattcg gctgatcgcc
      661 ggcgggctgt ccatgtccaa gcagatcatg tcgctcgagc gcggagtgca cgtgctggta
      721 gcgacgcccg gacgcctgag cgacctcatg cgccgtggca acgcggacct cagccaggtg
      781 cggatcacag tgctggacga ggccgaccac atggccgaga tgggcttcgc cgaggagatc
      841 accgagatcc tcgacaagac gtcgcccgat gcgcagcggt tgctgttctc ggccactctt
      901 gacggcgtgg tcgacaccct tgtcgcgcgt ttcatgacca atccggtgat gcacgatgtc
      961 accgacggaa acgcggcccc gagtcagcgc cacgtggtgc tgtccgtgcc gccgcacgag
     1021 aagtaccgga tggcgacccg catcgccgcg cgcgaggggc gcacgctgtg cttcgtgcgc
     1081 accaagctgg ccgccgaccg cgtcgcggcc gacatgcgct cccagggagt gctcgccgtc
     1141 gcgctgcatg gcgatcgcgc ccagaacgaa cgcaaccttg ccatgacgga gttccgcgcc
     1201 gggcgccttc ccgtgctcgt cgccactgac gtcgcggccc gtggaattca cgttgacgcg
     1261 gtcgaccttg tgttgcagat cgacccgccg gccgactatc gcgactacac ccaccgcgcc
     1321 ggccgcaccg ggcgcgctgg agccgacggc ctggccgtcc tcctgtcgct tccccaccag
     1381 cgcaacgcgg tacagcgcat cctcgaaaac gcgaacatca ccgcagagga cgagtccttc
     1441 cgcacgatcg acgcgatggc cctgcagcgc atcgaccaac tcaccggcgc ccgcgaaccc
     1501 accggtggct gggttgacga accgctcccc cctgcccgca acgaacgacg cggcagccgc
     1561 gggcgtcccg accgccaagg cggccgtggt gaccgccagg gcgggcgcgt ggaacgacgt
     1621 ccctcccgtc cctccggtcg acgcgatgag tcttccgcag gacgcgacca gatgaccgcg
     1681 cgcgaacgcg agttggcgga gcgcgaacgg tctctcgcag agcgcgaacg caggctcgca
     1741 caggcagagg cctcgcagtc cgctccctcg gccgcgcccg cttcggcacg ctccgacgag
     1801 cgccggtccg ctcccccgcg caagaccttc gatcgcgacg acaagcgtcc gccgcgcaag
     1861 acctttgacc gcgatgacaa gcgttctacc agggatgacg cgcgtccgtc atacgatgat
     1921 cggcgtccca cgagggacga caaacacccc gctcgcgatg atgcgcgacc ggcgtggaag
     1981 gactcccgac cgccgcgcga tgatcgcggc ggcgcccggg gcggcagtgc tccgcgctgg
     2041 gcagacaagg gcgcccgcga tgacacgcgc gctccccggg acgactcgcg cggcgaccgt
     2101 ggcgagcgcc ctgcgtaccg cggcaagccg gacagcgcg
//
LOCUS       DASRKH010000417         3012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_58302, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000417 DASRKH010000000
VERSION     DASRKH010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58302"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VFC06_06695"
     CDS             <1..560
                     /locus_tag="VFC06_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HZL81604.1"
                     /translation="ARRGGYAPGGEDYRWGIIRSRIPGWAFAVFNLVFIAVYQNALLL
                     AIALPAWTMVENPAPLGAYDVVAGALFLAFLVGETVADQQRWNFHRAHPEGGVLRTGL
                     FRYSRHPNYFFEIAQWWVVFAFAVVAAGNVWLPTVAGAALLTLLFLGSTAFTEWISKS
                     RHPEFEDYQHTTSMLVPWRPRRDAR"
     gene            620..1669
                     /gene="tdh"
                     /locus_tag="VFC06_06700"
     CDS             620..1669
                     /gene="tdh"
                     /locus_tag="VFC06_06700"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HZL81605.1"
                     /translation="MRALVKKASGPGLELADVPEPFAGDSDVTIRVLRTGICGTDLHI
                     ESWDQWAASTIKPPLVPGHEFSGEVVAVGKAVRDVHVGAIVSGEGHIVCGTCRNCRAG
                     RRHMCNRTSGLGVNRDGAFAEYVVLPESNVWVHPAGIGLDLAAIFDPLGNAVHTALKF
                     PLVGEDVLITGAGPIGLMAAAVVRHVGARFIVVTDVSEPRLELARLVGADLAVNVTSE
                     RIADAQERLGMREGFDVGLEMSGHATALPEMIENLTHGGRVAMLGLPSGPIDVDWAKV
                     VTHMLTISGIYGREMFETWNAMSAMLQTSVELRQALNTVITDRMPAGEWERGFATART
                     AHSGKIVLDWTEAPA"
     gene            1695..2882
                     /locus_tag="VFC06_06705"
     CDS             1695..2882
                     /locus_tag="VFC06_06705"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013600435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HZL81606.1"
                     /translation="MYGTMQAQLTAALDEIRDAGTMKGERPLASAQSSVVTTGGAEVL
                     NFCANNYLGLANDPRIIEAAKAGMDKWGFGLASVRFICGTQELHMELERRVSAFLGTE
                     DTILFSSCFDANGGVFEALFGPEDAIISDELNHASLIDGIRLSKARRMRYRNRDLADL
                     EDQLKAASDARQRIIVTDGVFSMDGYLAPLAGICDLAEKYRALVLVDDSHAVGFIGEH
                     GRGTPELLGVEGRIDIYTGTFGKALGGASGGYVSAPKEIVAILRQRARPYLFSNTVAP
                     AVVAGTLTALDLLEGGGELRRRLVDNAALFRSLMADAGFELLDGEHPIVPVMFGDASL
                     AARVADRMLTHGVYVVAFSYPVVPMGKARIRVQLSAAHSGAEIRRCVEAFVASRDEVL
                     AEA"
BASE COUNT          473 a          988 c         1019 g          532 t
ORIGIN      
        1 tcgctaggcg cggcggctac gctcccggcg gcgaggacta ccggtggggc atcattcgtt
       61 cgcgcatccc cggctgggct ttcgcagtgt tcaacctcgt cttcatcgcc gtctaccaga
      121 acgccctgct gctggccatc gcgctgcctg cgtggacgat ggtggagaat ccggcgccgc
      181 tcggcgccta cgacgtggtg gcgggtgcgt tgttcctagc gttcctcgtg ggcgagacgg
      241 tggcagacca gcagcgctgg aacttccatc gtgcacaccc cgagggcggc gtcctgcgaa
      301 ccggcctctt ccgatactcc cggcacccca actacttctt cgagatcgcg cagtggtggg
      361 tcgtgttcgc gttcgcggta gtggctgcgg ggaacgtgtg gctaccgacc gtggcgggcg
      421 cggcgctgct cacactgttg ttcctgggat ccaccgcgtt cacggagtgg atctcgaagt
      481 ctcgccaccc cgagtttgag gactaccagc acaccacctc gatgttggtg ccttggcggc
      541 cgcggcgcga cgcccggtga cagcgacata ctaggaacag cggcttgacg aacatcttgg
      601 ccgcagtgaa aggggcgatg tgcgcgcact agtcaagaag gcgtcaggcc ccggcctgga
      661 actcgccgac gtgcccgagc cttttgcggg cgactccgac gtcacgatcc gcgtgctgcg
      721 gactggaatc tgcggcaccg acctccacat cgagtcctgg gatcagtggg ctgcgtccac
      781 catcaagccg cctctcgttc ccggccacga gttcagcggc gaggtcgtcg ccgtcggcaa
      841 agccgtgcgc gatgtccacg tgggcgcgat cgtctccggc gagggccaca tcgtgtgcgg
      901 cacgtgccgc aactgccgcg cgggccgccg tcacatgtgc aaccgaacgt cgggcctcgg
      961 cgtgaaccgc gacggcgctt tcgccgagta cgtggttctg cccgagtcga acgtgtgggt
     1021 tcaccccgca ggcatcggcc tcgacctcgc cgcgatcttc gacccactcg gcaacgccgt
     1081 gcacaccgcg ctgaagtttc ccctggtagg agaggacgtg ctcatcacgg gcgcgggacc
     1141 gatcgggctc atggcggctg ccgtggtccg tcacgtcgga gcccgcttta tcgtcgtcac
     1201 ggatgtctcc gagccgcgac tcgagctcgc gcgcctcgtg ggagcagacc tcgcagtcaa
     1261 tgtgacgagc gagcggatcg ccgacgcgca ggagcgcctc ggcatgcgtg agggcttcga
     1321 cgtggggctc gagatgagcg gacacgcgac ggcactgccg gagatgatcg agaacctcac
     1381 ccacggcggc cgggtcgcga tgcttggcct gccgtcgggc cccatcgacg tcgactgggc
     1441 caaggtggtc acccacatgc tgacgatctc cggaatctac gggcgcgaga tgtttgagac
     1501 ctggaatgcc atgagcgcca tgcttcagac aagtgtcgag ctccgacagg ctctcaacac
     1561 cgtgatcacg gaccgcatgc ccgctgggga atgggagcgc ggattcgcga cggctcgcac
     1621 cgcacactcc ggcaagatcg tgctcgactg gaccgaggca cccgcctagc tggtcgccac
     1681 cgaagggaac actcatgtac ggaacgatgc aggcgcagct caccgcggcg ctcgacgaga
     1741 ttcgcgatgc cggcaccatg aagggcgagc gtcctctcgc gagcgcccag tcatcggtcg
     1801 tgacgaccgg tggcgccgag gtgctcaact tctgcgccaa caattacttg gggcttgcca
     1861 acgatccccg catcatcgag gccgccaagg ccggcatgga caaatggggg ttcgggctgg
     1921 cgagcgtacg gttcatttgc ggcacgcagg agttgcacat ggagctggag cgccgggtgt
     1981 ccgcctttct cggcacagag gacacgatcc tgttctcgtc gtgcttcgat gcgaacggcg
     2041 gtgtattcga ggcgctgttc ggtccggagg atgcgatcat ctccgacgag ctcaaccacg
     2101 cgtctctgat cgatgggatt cggctgtcca aggcgcgcag gatgcgttat cgcaatcgtg
     2161 acctcgccga cctggaggat cagctgaagg cggcgtcgga cgcgcgccag cgcattatcg
     2221 tcaccgacgg ggtgttttcg atggacggct acctcgctcc cctggcagga atctgcgatc
     2281 tggccgagaa gtaccgggcg ctcgtgcttg tcgacgactc gcacgccgtg ggattcatcg
     2341 gcgaacacgg ccgcggcacg ccggagctgc tgggcgtgga gggtcgcatc gacatctaca
     2401 ccggcacgtt cggcaaggcg ctgggcgggg cgtcgggcgg ctacgtctcc gcgcccaagg
     2461 agatcgtggc gatactgcgc cagcgggctc gcccttacct gttctccaac accgtcgcgc
     2521 ccgcggtggt ggcgggaact ctcaccgcgc tcgacctgct ggagggcggt ggcgaactgc
     2581 gccgccgtct cgtggacaac gcagccctgt tccgctcgct gatggccgac gcggggtttg
     2641 agctgctcga cggcgagcac cccatcgtgc cggtcatgtt cggcgatgcg tcactggcgg
     2701 cccgcgtggc ggaccgaatg cttacgcacg gcgtgtacgt cgtcgcgttt tcgtatccgg
     2761 tggtcccgat gggcaaggcg cgcatccgcg tgcaactgtc ggccgctcac tcgggcgccg
     2821 agatccggcg gtgtgtggag gcgttcgttg cgtcccgcga cgaggtgcta gcagaggcgt
     2881 agcgcccgtc agtccttgag aattgccgcc ccgagtcatt cagccggaat gcccagtccc
     2941 tcgacgagcg gcaacgcctg catgcgcgac tcccggcaca ggtcgtcgat ctgcgcgtcc
     3001 acggccttgg ac
//
LOCUS       DASRKH010000418         2898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_11150, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000418 DASRKH010000000
VERSION     DASRKH010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11150"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VFC06_06710"
     CDS             complement(<1..488)
                     /locus_tag="VFC06_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HZL81607.1"
                     /translation="MSPIPTASPTPPCAELHLHLEGTLEVPMVLELADRNGIRLPTPD
                     EDALRRRYAFTDLASFLALYYENTAVLRTAADFAALTGAYLRRASEAGVLHAEVMFDP
                     QAHVARGVPLGAVVEGVAAALEEGGREFGISTGLFAAFLRDRPATEAHAVLDELLALD
                     AP"
     gene            complement(672..1751)
                     /locus_tag="VFC06_06715"
     CDS             complement(672..1751)
                     /locus_tag="VFC06_06715"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZL81608.1"
                     /translation="MGRVAGGLVEVVVGAVALSAGAGIAVPHLTRAGLSATGAWATLA
                     VLAGLALLASGTRRLSEPLRWWGRLPTAVAVVAGAAVLLLTLGPAVAATTVPRTLLAP
                     TTPADRGLTFEEVTFATDDGVALSGWYLPSTNGAAVALLHGAGSTRTSVLDEAVVLAR
                     HGYGVLLFDARGHGRSEGRAMAYGWNGDADTMAAVTFLTGRPDVQADRIGVVGLSMGG
                     EQAIGALAADPRIRAAVAEGATGRTAADNAWFSEVYERRGSAQEMIDRLRFTFVDLLT
                     EAPRPPSLRESAAAAAPRPILLIAAGEVPDEQHAAAYIRSGSPASVEVWVVAGARHTG
                     GLATAPSEWRTRVTAFLDGALPPPA"
     gene            1844..2563
                     /locus_tag="VFC06_06720"
     CDS             1844..2563
                     /locus_tag="VFC06_06720"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZL81609.1"
                     /translation="MDNGASVRTGVSHPAVIMIIEGRVPEGGQASLEEFFAAPPAFFD
                     APHSALMHIQWNAADGRTFRVAFKYATERDFEIEDIRTRTESAVAQHRARLARLLDAQ
                     PVITIWRESSRQFSLDLVRAYWDLVAAREWEALRAIVAPDVVIEWPASNERLAGVEDL
                     IAANRGAPDGWTAKVRSVVGSGENVASDVEEFLDSGESFRVATFWTVRNQRIVRGTEY
                     RTAVGQSHTPAWRRGHTRPIL"
     gene            complement(2573..>2898)
                     /locus_tag="VFC06_06725"
     CDS             complement(2573..>2898)
                     /locus_tag="VFC06_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HZL81610.1"
                     /translation="PVLVVGEEADVVAPAAPLTVEEAAGLRALMRGVVETGTSTFLQD
                     VPGEPVGAKSGTAQFGTTDPPSTHAWMIAFQGDLAVAVFVEVGDYGTATAGPILEAFL
                     RGAAG"
BASE COUNT          433 a         1052 c         1067 g          346 t
ORIGIN      
        1 atgggggcgt ccagggcgag tagctcgtcg aggaccgcgt gggcctcggt cgccgggcgg
       61 tcgcgcagga aagccgcgaa cagccctgtg ctgatcccga actcgcggcc gccctcctcg
      121 agggcggcag ccaccccttc caccaccgcg ccgagcggga caccgcgggc aacgtgggcc
      181 tgcgggtcga acatgacctc ggcgtgcagc acccccgcct ccgacgcgcg ccgcaggtac
      241 gcaccggtga gcgccgcgaa gtccgccgcc gtccggagga ccgcggtgtt ctcgtagtag
      301 agcgcgagga aggaggcgag gtcagtgaag gcgtaccgcc ggcggagcgc gtcctcgtcc
      361 ggcgtcggga gccggatccc gttgcggtcc gccagttcca gcaccatggg aacctcgagg
      421 gtgccctcca ggtggaggtg aagctccgca cacggcgggg tgggggacgc ggtggggatc
      481 gggctcatga aggccatcgt cccccaagtg gcgccagttc tggcggcagt tcgccgcgct
      541 ccgggacgac ggcgccgcgg agcgcgccaa ccaacagctc gagcagagcc ggaaggcggc
      601 ggacgactac atccgcgacg tcgcccgcta cgccctggcc acccgctgac tacggcggct
      661 cgacggggtg ctcaggccgg tggcggcagc gccccgtcga ggaacgccgt cacccgcgtc
      721 cgccactcgc tgggtgccgt cgccagaccg cctgtgtgcc gcgcgccggc aacgacccac
      781 acctcgacgg acgccgggga gccggaccgg atgtatgcgg ccgcgtgctg ctcgtccggg
      841 acctcgcccg ccgcgatgag caggatcggc cgcggggcgg cggccgccgc ggattcccgc
      901 aacgagggcg ggcgcggcgc ctcggtgagg aggtcgacga acgtgaatcg gagccggtcg
      961 atcatctcct gggcactgcc ccgcctctcg tacacctcgg agaaccaggc gttgtccgca
     1021 gccgtccgcc ccgtcgcgcc ctcggcgacc gcggcccgga tgcgcgggtc cgccgcgagc
     1081 gcaccgatcg cctgctcgcc tcccatggac agcccgacga cgccgatccg gtccgcctgc
     1141 acgtccgggc gcccggtgag gaaggtgacg gccgccatgg tgtcggcgtc gccgttccag
     1201 ccgtaggcca tcgcccgccc ctcgctgcgg ccatggccgc gcgcgtcgaa gaggaggacg
     1261 ccgtagccgt ggcgggccag gacgacggcc tcgtcgagca cgctggtgcg tgtcgacccg
     1321 gcgccgtgga gcagcgcgac cgccgcaccg ttcgtggacg ggaggtacca gcccgagagc
     1381 gcgacgccgt cgtcggtggc gaaggtgacc tcctcgaacg tgaggccacg gtcggcgggc
     1441 gtggtggggg cgaggagggt tcgtggcacg gtggtcgcgg cgacggccgg gccaagggtg
     1501 aggaggagca ccgcggcccc ggcgacgact gccaccgccg tcgggagcct gccccaccag
     1561 cgcagtggtt cgctcagccg gcgggtgccc gacgcaagga gggcgagacc ggcgagcacg
     1621 gcgagcgttg cccacgcgcc cgtcgcggac agtcccgccc tggtgagatg cgggacggca
     1681 atgccggcgc cggccgagag cgcgacggcg ccgaccacga cctcgacaag gccgccggca
     1741 acccgaccca caaccgatct cctcatcggg tcccggtcaa ctggggttga accggaggac
     1801 aatggagaca caagagcact caaccaccag atcggggtgc gagatggaca atggcgcatc
     1861 ggtccgcacg ggagtcagcc acccagccgt catcatgatc atcgagggtc gggtcccgga
     1921 gggcggccag gcctccctcg aggagttctt cgccgcgcca cccgccttct tcgacgcccc
     1981 gcacagcgcg ctcatgcaca tccagtggaa cgccgccgat ggcaggacgt tccgcgtggc
     2041 cttcaaatac gcgaccgagc gcgacttcga aatcgaggac atccgcaccc ggacggagtc
     2101 ggccgtggcc cagcaccgcg cccggctcgc tcgcctgctg gacgcccagc ccgtcatcac
     2161 gatctggcgg gagtccagcc ggcagttcag cctggacctg gtcagggcct actgggacct
     2221 cgtggccgcc cgcgagtggg aggcgctccg cgccatcgtg gcgcccgacg tcgtgatcga
     2281 atggcccgcc tcgaacgagc gcctcgcggg ggtggaggac ctgatcgccg ccaaccgcgg
     2341 cgcccccgac ggctggacgg ccaaggtgcg ctcagtcgtc ggcagcggcg aaaacgtggc
     2401 gagcgacgtg gaggagttct tggacagcgg cgagtcattc cgggtcgcca cgttctggac
     2461 ggtccggaac cagcggatcg tcaggggcac ggagtacagg acggctgtcg ggcagagtca
     2521 cacgccggcc tggcggcggg ggcacacccg cccgatcctg tgagctgagc gttcagcccg
     2581 cggcgccccg caggaacgcc tcgaggatcg ggccggcggt cgcggtcccg tagtcgccca
     2641 cctccacgaa cacggcgacg gcgaggtcgc cctggaacgc gatcatccag gcgtgcgtcg
     2701 acggcgggtc ggtggtgccg aactgcgccg tcccgctctt cgcgcccacc ggctcgccgg
     2761 gcacgtcctg caggaaggtg gacgtcccgg tctcgacgac gccgcgcatc agcgcccgca
     2821 ggccggccgc ctcctccacg gtgagggggg ccgcaggggc gacgacgtcg gcctcctcac
     2881 ccaccaccag cacgggcg
//
LOCUS       DASRKH010000419         2662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_12578, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000419 DASRKH010000000
VERSION     DASRKH010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12578"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(331..2070)
                     /locus_tag="VFC06_06730"
     CDS             complement(331..2070)
                     /locus_tag="VFC06_06730"
                     /inference="COORDINATES: protein motif:HMM:NF016232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZL81611.1"
                     /translation="MLDRVVQDALDAIRDGVTAHNQESVRLDFKTVGRSLPDTLKNLA
                     EAAACFANAGGGTIVVGVRNDVPGSSAFVGSILLPEATVARIYELTEPGLIVITEQYP
                     YEGVSLTVITVPRSPDIHQVAGRSTERVGTSCEPMTASRIATVLADRRGDDWSAKNSG
                     VPVSEASPVAEEIARELLAQSSDAERHSWARLPWPDLCRRLGVVTEDHLNNAGALLFT
                     DSRIHVQYLRRTTSTGLLSANESIKGSGVWAIRRVLEMIENRTERTAIVLPGGSQLFV
                     GDLPDGAVREAIVNAFMHRDYRSSDTIQIEHQGSRLRVTSPGGFVAGVTVDNVLTVSS
                     RSRNLALSQAIRGMGLAESAGVGVDRMYAAMTAVGHEPPTFFTDGLTVEAVLQGGPPN
                     EPVTRFVAELSEDRRGDPDTLLVLTFLLRRRTTTAARMAPLMQKSVVEAEARLMDLSA
                     PGDALIERTADSARSKFGEYRLVGEAVRSLGAAVTYRARSGDDTDRKVIAIVREMGSI
                     NGRVVQTMFDVQPATASRILSDLVDREVLLKTSSATRGPSVTYGPGRRFPKDKGKRAR
                     ETSAEQQILDLGD"
     gene            2355..>2662
                     /locus_tag="VFC06_06735"
     CDS             2355..>2662
                     /locus_tag="VFC06_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81612.1"
                     /translation="MGTCPRSGMMDSEMYSQEIPELRLPAEQRARALIDAQLTAAGWA
                     VQDKRDLNLFAAEGVAVREFTLAPGHGRADYLLFVDQRAVGRDRGQTAGHAAGRRGVA
                     "
BASE COUNT          426 a          889 c          867 g          480 t
ORIGIN      
        1 gggtttccac cgacggtgag ccgcgcgcgc cgcggtctcg gcgcggaacc tccccctcgg
       61 cgtggagggg ggcactgccg ggtgccgacc gcggctccat ccgcgcgcgg ctgacgccgg
      121 gcgctcccgc caggcccggc cacgccgcgg cgcaccaccc gtcagcgccc cttgttctgg
      181 ccgaattctg gccgagaggg agggtttcca ccgaggggga gccgtgttcg cagcggtctc
      241 ggcgcggaac ctccccctcg gcgtggaggg gggcgctgcc gggtgccgac cgcggctcca
      301 tccgcgcgcg gctgacgtcg ggcgcgcctc tcagtcgccg agatcgagaa tctgttgctc
      361 ggcagacgtc tcccgcgctc gcttgccctt gtccttgggg aatcggcgcc ctggaccgta
      421 agtcacgctg ggtccacgtg ttgcgctgct cgtcttgaga aggacctccc ggtccacaag
      481 gtcagacagg atccgtgaag ccgttgctgg ctggacgtcg aacatcgttt gaacgaccct
      541 cccgttgatg gaacccatct cccgaacgat cgcgatgacc ttgcggtcgg tatcgtcgcc
      601 cgagcgcgcg cggtaggtga cggccgcgcc gagcgagcga acggcctccc ccaccagtcg
      661 gtactccccg aatttgctgc gtgctgagtc cgcggtccgt tcgatcagcg cgtctcctgg
      721 agcggagaga tccatcagcc tcgcttccgc ctccaccacg ctcttctgca tgaggggagc
      781 cattcgtgcc gcggtggttg tccggcgcct caggaggaac gtgagcacga gtagagtgtc
      841 cggatcccca cgccgatcct cactgagttc cgcaacgaat cgagtaaccg gctcattggg
      901 gggcccgcct tggagaacgg cttcgacggt caaaccatcc gtgaagaacg tgggtggctc
      961 gtgcccaaca gctgtcatcg cggcatacat ccggtcgacg ccaacaccgg cagactcggc
     1021 caaacccatg cctcgaatcg cttgggacag tgccaggttg cgggatcgcg acgaaacggt
     1081 gagcacgttg tccacggtca cccccgccac gaaccccccg ggtgacgtca cgcgtagccg
     1141 cgacccctgg tgctcgatct ggatggtgtc cgaagagcga tagtcacggt gcatgaaagc
     1201 gttcacgatc gcctcacgaa ctgctccgtc cggcagatct ccgacgaata gttgtgatcc
     1261 tcccggcaat acgatcgcag tccgttcagt gcgattctcg atcatctcga gcacgcggcg
     1321 gatggcccac actccagatc ccttgatgct ctcgtttgcc gagagcaagc cggtacttgt
     1381 cgtgcggcgg aggtattgca cgtgaatccg actgtccgtg aacagaaggg cgcccgcgtt
     1441 gttgaggtgg tcttcggtca ccactccgag ccgcctgcag agatcgggcc acggcagtcg
     1501 ggcccaagaa tgccgctcgg catcggacga ctgtgcgagg agctcgcggg cgatctcctc
     1561 cgcgaccggg gaggcctccg agaccggtac gcccgagttc tttgccgacc agtcatcgcc
     1621 tcttcggtca gccaggaccg tggcgattct tgatgctgtc attggttcgc aggaggtacc
     1681 gacccgttcc gtggagcggc cggccacttg gtggatgtcg gggcttcggg gcacggtgat
     1741 gactgtgagg ctcacaccct cgtacgggta ttgctctgtg atcacgatga gtcccggctc
     1801 cgtgagttcg tagattcttg cgaccgtcgc ctcggggaga aggatggatc caacgaaggc
     1861 agaagatccc ggaacgtcgt tccggacgcc tacgacgatc gttcctccac cagcgttggc
     1921 aaagcacgcg gcagcctccg caaggttctt cagtgtgtcc ggtagggaac gtcccaccgt
     1981 cttgaagtcg agccggacac tctcctggtt atgcgccgtc acaccatcgc ggatggcatc
     2041 gagcgcatcc tggactactc ggtcgagcac gagtttctcc tagttttgat caactccctc
     2101 ctgtcatcct aggtcaaaac tcaggaaaac aacggaggct gggggccgct gccgggtgcc
     2161 gaccgcgcct ccatccgcgc gcgacttcgt cgggcgctcc cgccaggccc ggccacgccg
     2221 cggtgcgtca tccgcccgcg cctcctcgtt gggccgccgc acgttcgcgt cgcctttggg
     2281 cgcagcccaa cgcgttgggg taccaccgaa ttctcggaga ggcagcgccg atatgtcatc
     2341 gaaggtgact gtccttgggc acgtgtcccc ggtcgggcat gatggacagt gagatgtatt
     2401 cccaagaaat ccccgaactg cggcttccgg cggagcagcg cgcccgcgct ctcattgacg
     2461 cgcagctcac ggctgcgggg tgggcggtgc aggacaagcg ggacctgaac ctgttcgccg
     2521 ccgagggcgt cgccgtgcgg gagttcaccc tcgcgccggg ccacggccgc gccgactacc
     2581 tgctgttcgt ggaccagcgc gccgtggggc gtgatcgagg ccaaaccgca gggcacgccg
     2641 ctggccggcg tggagtggca at
//
LOCUS       DASRKH010000420         3081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_38117, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000420 DASRKH010000000
VERSION     DASRKH010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38117"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..920
                     /locus_tag="VFC06_06740"
     CDS             21..920
                     /locus_tag="VFC06_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005267969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HZL81613.1"
                     /translation="MTTTALRVLAAIALATTATTACSTDSGADSGAVPPGVSVAAAFY
                     PLAFVAERIVGDVGTVTTVTAPGVEPHDVELSPMTVREMQSTDVVLYVSGFQPAVDDA
                     IESTGSFGLDASAVVNLHAADETAVHGDADPHFWLDPALLATYALAVGDSLAQLDPAH
                     GDTYVTNAADLADELTALADSYATGLSQCARRDIVVSHEAFGYLAGAFDLNQVAIAGV
                     DPQNEPSPAKIREIRDLITSTGSTTVFTESLVNPAVAESLAADAGVATAVLNPVETVV
                     DGDDYLGVMNRNLDALRKALDCA"
     gene            913..1710
                     /locus_tag="VFC06_06745"
     CDS             913..1710
                     /locus_tag="VFC06_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZL81614.1"
                     /translation="MPESREPDRVAPPALTARDVHVSLNGRPILHGVGIDVSPGEVVA
                     LMGGNGSGKSTLVRALVGAVPLAEGSVTLFDAPATASARARVGYVPQRMSAAGGVAAT
                     VDEVVRSGLLGHRRLRGPRDAHVRAKQALSLLGIEDLLWRDVSRLSGGQQQRVLVARA
                     LVRRPDLLILDEPMAGVDLQSQVAFAHALGHLKENGVAVIIVLHELGALARHIDRSVV
                     LEGGRVIHEGAPPEDLGVHAIPGHEHEHAHADPPPLARGDIVLEGPA"
     gene            1707..2555
                     /locus_tag="VFC06_06750"
     CDS             1707..2555
                     /locus_tag="VFC06_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HZL81615.1"
                     /translation="MSLLADPLVQRMLLVGLLVGAAAPIVGTYLVQRRMALLGDGIGH
                     VALAGVAAGWLAGSAAGLAQRDALAVPGAVLFAVVGAVIIERMRARGAAADVVMAILF
                     YGGIATGVLLIGAAGGSNANLLGYLFGSISSVGWVDVWITAALAIVIMGVGLGLRGAL
                     FAVTHDQEFARSTGLPVEVLNMIVAVLAAVTITVAMRVVGVLLVSALMILPVAIAQRL
                     TASFSRTMYVAMGIGAALTLTGIWITLTQNLQPGALIVVLGVGAYVLVTLVVAARESV
                     RRGVRS"
     gene            2552..2956
                     /locus_tag="VFC06_06755"
     CDS             2552..2956
                     /locus_tag="VFC06_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016664471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HZL81616.1"
                     /translation="MTEAKPRQTKQRVAILVALGQGGFRSAQDWHDLLRHGGATIGLA
                     TVYRALQALAESGEVDAVLTDSGETLYRLCAAREAHHHHLRCRVCGAAEDVDMPTFEA
                     WAASVATQHGYSRLEHTVELTGVCGRCAAEGN"
BASE COUNT          449 a         1061 c         1020 g          551 t
ORIGIN      
        1 gtatgactag tgtcgttgtc atgacgacca ccgccctgcg cgttctcgcg gcaatcgcgc
       61 tcgcgaccac agcgaccacc gcatgctcca cagactccgg cgccgactcc ggcgcggtac
      121 cgccaggcgt gagcgtcgcg gccgccttct acccactcgc attcgtagcc gagcgcatcg
      181 tgggcgacgt aggcactgtc acgacggtga ctgctcctgg cgtcgagccg cacgacgtgg
      241 aactctcccc catgaccgtc cgtgagatgc aaagcaccga cgtggtcctc tacgtctcgg
      301 gtttccagcc cgccgtggat gatgcgatcg agtccaccgg aagcttcggc ctcgacgcga
      361 gcgcggtggt caaccttcac gcggccgacg agacggcggt tcacggcgac gccgacccgc
      421 acttctggct cgaccccgcg ctactcgcca cctatgcgct tgccgtcggc gactcactcg
      481 cccaactgga tcccgcgcac ggcgacacgt acgtgacgaa tgcggccgac ctcgccgacg
      541 aactcaccgc cctcgcggac tcgtacgcca cagggctgtc tcagtgcgcg cgccgcgaca
      601 tcgtcgtcag ccacgaggcg ttcggctatc tggccggggc cttcgacctg aaccaggtcg
      661 ccatcgccgg cgtcgacccc cagaacgagc cctcgcccgc caagatccgc gagatccgcg
      721 acctcatcac ctcgaccggg tcaaccacgg tcttcaccga gtcgctcgtc aacccggccg
      781 ttgcggagtc gcttgcggcc gatgccgggg ttgccaccgc cgtcctcaat ccggtggaga
      841 ccgtcgtcga cggcgacgac taccttggtg tcatgaatcg gaacctggac gccctgagga
      901 aggccctcga ctgtgcctga gtctcgcgag ccggatcgcg tcgcgccgcc cgcgttgacg
      961 gcgcgggacg tgcacgtgtc gctcaatggc aggccaatcc tgcacggggt cggcatcgac
     1021 gtctccccag gcgaagtcgt cgcgctcatg ggtggcaacg gctccggcaa gtccactctg
     1081 gtccgggcac ttgtgggcgc cgttccgctc gccgaaggct cagtcacact gtttgacgcc
     1141 cccgcgactg cgagcgcgag agcccgagtc ggctacgttc cccaacggat gagcgccgct
     1201 ggcggcgtcg cagcgaccgt ggacgaggtc gtgagatccg ggctcttggg gcaccgcaga
     1261 ctgcgcggcc cgcgtgacgc gcacgtccga gccaagcaag ccctttctct cctcggcatc
     1321 gaagatctcc tgtggcgcga cgtcagccga ctttctgggg gccaacaaca acgggtgctc
     1381 gttgcgaggg ccctggtgcg tcgccctgac ctcctcattc ttgacgagcc gatggcgggc
     1441 gtggatctgc aatcgcaagt tgcgttcgcg catgcgcttg ggcacctcaa ggagaacggc
     1501 gttgccgtca tcatcgtgct gcatgaactt ggcgcactcg cgcggcacat tgaccgctcg
     1561 gtggtgctgg agggcggccg cgtgatccac gagggcgcgc ctcccgaaga cctcggagtg
     1621 catgcgattc ccggacacga gcacgagcac gcccatgcag atcccccacc cctggcgcgc
     1681 ggcgacatcg tcttggaggg accagcatga gcctccttgc ggaccccttg gtccagcgca
     1741 tgttgttggt cggcttgctc gtgggagcgg ccgcccccat cgtcggcacg tacctggtgc
     1801 agcgccgcat ggcgctgctc ggtgacggta tcggacacgt cgcgctggcg ggcgtcgcgg
     1861 ctggctggct cgcaggctcg gcggcggggc ttgctcaacg agatgccttg gccgtccccg
     1921 gcgcggtcct gttcgcggtg gtcggcgcgg tcatcatcga acgcatgcgt gcccgaggcg
     1981 ccgctgcaga cgtcgtcatg gccatcctgt tctatggagg catcgcgacg ggggtgctgc
     2041 tgatcggcgc tgcgggcggc tcgaacgcca acctgctggg ctacctgttc ggttccatct
     2101 cttcggtcgg atgggtcgat gtgtggatca cggcggcgct cgcgattgtc atcatgggcg
     2161 tcgggctcgg gttgcgcggc gcgctctttg cggtgacgca cgaccaagag ttcgcgcgct
     2221 cgaccgggct gccagtcgag gtgctcaaca tgatcgtggc tgtgctcgcc gccgtcacca
     2281 tcaccgtggc aatgcgcgtg gtcggagtgc tcctggtctc ggccctcatg atccttcccg
     2341 tggcgatcgc ccaacgactc acggcgtcct tctcccgcac gatgtacgtt gcgatgggta
     2401 tcggagccgc actcaccctg acgggaatct ggatcaccct cactcagaac cttcagccag
     2461 gcgcgttgat cgtggtgctc ggagtgggcg cctacgtcct cgtgaccctg gtggtcgcgg
     2521 cccgcgagag cgtccgacgg ggggtgagat cgtgaccgaa gccaagcctc gccagaccaa
     2581 gcagagagtc gcgattttgg tggcgctcgg ccagggtggt ttccgttcgg cccaggattg
     2641 gcacgacctc ctgcgtcacg gcggcgccac catcgggctg gcgaccgtct accgcgcgct
     2701 ccaggctctc gccgaatcgg gagaagtgga cgcagtgctg accgactcag gcgagacctt
     2761 gtaccgactg tgcgcagcac gcgaggcgca ccaccaccac ttgcgatgcc gcgtgtgcgg
     2821 tgccgctgag gacgtggaca tgcccacctt tgaggcgtgg gcggcgtcgg tggccaccca
     2881 gcacggctat tcccgcctcg agcacacggt agaactcacc ggcgtgtgcg gccgatgtgc
     2941 agcggagggg aactagatgg cgggcgtccc cagcttcatc tccgatggac cgtggctcgg
     3001 actgttcgtc tttctgctcg ttgtcgtctt cttccgcgcc cagggcacct actggttggg
     3061 gcggtgggtg cgcgggcgtg c
//
LOCUS       DASRKH010000421         2210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_57260, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000421 DASRKH010000000
VERSION     DASRKH010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57260"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="VFC06_06760"
     CDS             complement(<1..917)
                     /locus_tag="VFC06_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HZL81617.1"
                     /translation="MTSANLGMAHTSSRAAILDVIRAAGTISRVELTRATGLTGATIS
                     TVVRRLINEGLVLEIGRAESTGGKPRMLLQLDPTARFAIGVHLDHAGITYVVANLGGA
                     IVTRWRKPGAGSDSPAEVVARIVREIDGMIARTGIDRGRVLGLGVVSPGPITATVGMV
                     LTPPSMEPWTEYPLGDALEDGVGVPVLLDNDATAAAIGEYWAGGIEPASAFAALYMGT
                     GIGAGVLVNGTVYRGASSNSGEIGHICVEIDGPPCWCGSRGCVEILAGPAAVVTAARA
                     AGLDVGPTERPVSESFAVVARAALRGEPTA"
     gene            1156..>2210
                     /locus_tag="VFC06_06765"
     CDS             1156..>2210
                     /locus_tag="VFC06_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZL81618.1"
                     /translation="MFRPARRTRLLTVAAVASLISLTTACAGGGDATPADTASSPGGS
                     AAAAAPSGDITVLTWRTDLVTDGTFDKYKAAFEAKYPEVKVSFEGITDYEGEVKTRMN
                     TTDYGDVLAIVNTITPAQMPDFFEPLGDATEMQKTYRFLTPKIFEGKVYGIPVVGNTQ
                     GIVYNKKVWAAAGITAVPTTSEEFLKDLKAIKDKDSSVVPLYTNYKDGWPLTQWESHR
                     GEISADPEFDSKLGQDDAPWAAGTDHFVIDSLLYDAVNQKLTEADPTTTNWEESKNLI
                     ATGKVSAMTLGSWAIAQMQKAATDAGNPATDIGYMPFPHQTDGKFYATVGGDYAQAIN
                     VNSKHKEAARAWIDWFNT"
BASE COUNT          387 a          807 c          718 g          298 t
ORIGIN      
        1 gcggtcggct caccgcgcag cgcagcccgc gcgacgaccg cgaagctctc cgagacgggc
       61 cgctcggtcg gaccgacgtc gagccccgca gcccgtgcgg cggtgacgac cgcagcgggc
      121 ccggccagga tctcgacgca gccccggctc ccgcaccagc agggcggtcc gtcgatctcg
      181 acgcagatgt gtccgatctc accggagttc gagctggccc cccgatacac cgtgccgttg
      241 acgagcaccc cggcgccgat gccggtcccc atgtagagcg cggcgaacgc ggacgcgggc
      301 tcgatgccac ccgcccagta ctcaccgatc gcggcggcgg tcgcgtcgtt gtcgagcagc
      361 acgggcaccc cgacgccgtc ctcgagggcg tccccgagcg ggtactccgt ccacggctcc
      421 atgctcgggg gcgtcagcac catgccgacg gtcgctgtga tcggtccggg cgacacgacg
      481 ccgagcccga gcacgcggcc gcggtcgatc ccggtgcgcg cgatcatgcc gtcgatctcg
      541 cggacgatgc gggcgacgac ctccgcgggg ctgtcggagc cggcacccgg cttgcgccac
      601 cgcgtgacga tcgcgccgcc gaggttcgcg accacgtagg tgatgcctgc gtggtccagg
      661 tgcacgccga tggcgaaccg cgcggtcggg tcgagctgca ggagcatgcg gggcttgccg
      721 cccgtcgact cggcgcggcc gatctcgagg acgagcccct cgttgatcag gcgccggacg
      781 acggtcgaga tcgtggcgcc ggtcaggccc gtcgcgcgcg tgagctcgac gcggctgatc
      841 gtccccgcgg cgcggatgac gtcaaggatc gccgctcgac tgctggtgtg cgccatcccg
      901 agattggcac tggtcacgca cttcctcctg tctccgcgca caggctgtgc gcgcgtcgcg
      961 cacgacccgc tgcgccaggt ccccaacgta acgatctggc gacgacagcg gtgacggacg
     1021 acacgcggat tgacttactt tgttcggctg cctaagaatg tcacacagag cggtggcgac
     1081 caacgccacc gcccgggcac tgaagcccaa gccccgccgg cacgtcgagg tgccgggctg
     1141 cagagaggat caacgatgtt ccgtcccgca cgacgtacgc gactgctgac cgtcgccgcc
     1201 gtcgcttcac tcatctctct caccacagcg tgcgccggcg gaggagacgc cactcctgcc
     1261 gacaccgcga gcagcccggg cggctcggcc gccgctgccg ctccgtcggg tgacatcacc
     1321 gtcctgacgt ggcggaccga cctcgtgaca gacggcacct tcgacaagta caaggccgcg
     1381 ttcgaggcca agtaccccga ggtcaaggtc tccttcgagg ggatcacgga ctacgagggc
     1441 gaagtcaaga cccgcatgaa cacgaccgac tacggcgacg tgctggccat cgtcaacacg
     1501 atcacgcccg cccagatgcc cgacttcttc gagccgctcg gcgacgcgac cgagatgcag
     1561 aagacctacc gcttcctcac cccgaagatc ttcgagggca aggtctacgg catcccggtc
     1621 gtcggcaaca cgcagggcat cgtctacaac aagaaggtct gggcggccgc cggcatcacc
     1681 gcggtgccga ccacgtccga ggagttcctc aaggacctca aggcgatcaa ggacaaggac
     1741 tcgtccgtcg tcccgctcta cacgaactac aaggacggct ggcccctcac gcagtgggag
     1801 tcccaccgcg gcgagatctc ggccgatcct gagttcgaca gcaagctggg ccaggacgac
     1861 gccccgtggg ccgcgggcac cgaccacttc gtgatcgact cgctgctgta cgacgccgtc
     1921 aaccagaagc tcaccgaggc cgacccgacg acgaccaact gggaagagtc caagaacctc
     1981 atcgcgaccg gcaaggtctc cgcgatgacg ctcggctcgt gggccatcgc gcagatgcag
     2041 aaggccgcga ccgacgcggg caacccggcc acggacatcg gctacatgcc gttcccgcac
     2101 cagaccgacg gcaagttcta cgcgaccgtc ggcggtgact acgcccaggc catcaacgtg
     2161 aactccaagc acaaggaggc cgcgcgcgcc tggatcgact ggttcaacac
//
LOCUS       DASRKH010000422         2328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_45544, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000422 DASRKH010000000
VERSION     DASRKH010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45544"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2328
                     /gene="infB"
                     /locus_tag="VFC06_06770"
     CDS             <1..>2328
                     /gene="infB"
                     /locus_tag="VFC06_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HZL81619.1"
                     /translation="PAGAAPAPAPRPAPKPAAAPVEANAAPAVAAPAPVVSAPAASVP
                     APSAPSAPAPLTPAPTVAAPSAAAPGTTTPAPSALPRTAAPAADAGRPGPRPAPTRPS
                     GSRPGNNPFAPSQGMGRGERPSGPGGSGGPRSGGPRPGNNPFAPSQGMPRPGERRTEA
                     PGASGAPAGASERPGGPRPGGPRPNPGMMPGRSASGVGRPGERPGPARAGGGARPGGG
                     GYAGRPGGAGGPGGAPGGAPGGGGGFAGRPGGGGGRPGGAGRGSTQGAFGRAGGRPVR
                     GRKSKRAKRQEFEQMQAPSLGGVQVPRGNGKTVVRLRHGSSLNDFADKIDANPASLVT
                     VLFHLGEMATATQSLDEDTFGTLASELGYIIEMVSAEEEDRELLGSFDIDLDAELADE
                     GDEQLLARPPVVTVMGHVDHGKTKLLDAIRQTDVVAGEAGGITQHIGAYQVRTVHEGT
                     DRAVTFIDTPGHEAFTAMRARGAQVTDIAILVVAADDGVMPQTIEALNHAQAAGVPIV
                     VAVNKVDKEGANPAKIRQQLTEYNLVAEEYGGDTMFVDVSAKLNQGIDQLLEAVLLTA
                     DASLDLRANPNKDARGVAIEANLDKGRGAMATVLVQSGTLHVGDAIVAGTAHGRVRAM
                     FDEHGNTLSEAGPARPVVVLGLASVPRAGDTFLVAPDERTARQIAEKRESAERAALLA
                     KRRKRISLEDFTQALQLGKVETLNLVLKGDVSGAVEALEDALLKIDVGEEVELRVIHR
                     GVGAITQNDVNLATVDNAIIIGFNVKFGERVEDLA"
BASE COUNT          330 a          854 c          840 g          304 t
ORIGIN      
        1 accccgcagg tgccgctccg gcacccgctc cgcggcccgc cccgaagccg gctgctgcgc
       61 ccgtcgaggc gaacgccgcc ccggctgtcg cggcacccgc tcccgtggtg tcagcccctg
      121 cggcctctgt ccctgccccg agcgcgccca gcgccccggc acccctgact ccggcgccga
      181 ccgttgccgc accgagcgcc gctgcgcccg gcacgacgac gccggcaccc tcggcactcc
      241 cgcggacggc cgcaccggcc gccgacgccg gccgtcccgg tccgcgaccg gcgcccacgc
      301 gtccgagcgg ctcgcggccg ggcaacaacc ccttcgcgcc ctcgcagggc atgggtcgcg
      361 gcgagcgtcc gtccggaccc ggtgggtccg gtggcccccg ctccggtggc ccgcgcccgg
      421 gcaacaaccc gttcgctcct tctcagggca tgccgcgacc cggcgagcgc cggaccgagg
      481 cccccggggc gagcggcgca cctgccggcg cgagcgagcg tccgggcggt cctcgtcccg
      541 gtggcccgcg tcccaacccg ggcatgatgc ccggccgtag cgcgagcggc gtcggccgtc
      601 cgggcgagcg tccgggtcct gcgcgtgccg gcggtggcgc acgtcccggc ggcggcggtt
      661 acgccggtcg tcccggtggc gcaggcggtc ccggtggagc gcccggtggc gcgccgggtg
      721 gcggcggcgg tttcgccggt cgtcccggtg gcggcggtgg ccgtccgggt ggtgcgggtc
      781 gtggctcgac ccagggtgcg ttcggtcgtg caggagggcg ccccgtccgg gggcgcaagt
      841 ccaagcgggc gaagcgccag gagttcgagc agatgcaggc cccctcgctg ggtggcgtgc
      901 aggttcctcg cggcaacggc aagacggtcg tgcgcctgcg tcacggctcg tcgctcaacg
      961 acttcgcgga caagatcgac gctaaccccg cgagcctcgt gaccgtgctg ttccacctcg
     1021 gtgagatggc gacggcgacg cagtcgctcg acgaggacac gttcggcacg ctcgcatcgg
     1081 agctcggcta catcatcgag atggtctcgg ccgaggagga ggaccgcgag ctgctcgggt
     1141 ccttcgacat cgacctcgac gcggagctcg cggacgaggg cgacgagcag ctgctcgcgc
     1201 gcccgccggt ggtcaccgtc atgggtcacg tcgaccacgg caagaccaag ctcctcgacg
     1261 cgatccgtca gacggacgtc gtcgcgggcg aggccggcgg gatcacccag cacatcgggg
     1321 cctaccaggt ccgcaccgtg cacgagggca ccgaccgtgc ggtgacgttc atcgacaccc
     1381 cgggtcacga ggccttcacg gcgatgcgtg cgcgtggtgc gcaggtcacc gacatcgcga
     1441 tcctcgtggt cgcggccgac gacggcgtga tgcctcagac gatcgaggcg ctcaaccacg
     1501 cccaggccgc cggtgtcccg atcgtggtcg ccgtgaacaa ggtggacaag gagggggcca
     1561 accccgccaa gatccgccaa cagctcaccg agtacaacct ggtggccgag gagtatggcg
     1621 gcgacacgat gttcgtcgac gtgtcggcga agctcaacca ggggatcgac cagctgctcg
     1681 aggctgtgct gctcacggca gacgcgtcgc tcgacctgcg ggccaacccg aacaaggacg
     1741 ctcgcggcgt cgcgatcgag gccaacctcg acaagggccg cggcgccatg gcgaccgtgc
     1801 tcgtgcagtc cggcacgctc cacgtgggtg acgcgatcgt cgcgggcacc gcacacggac
     1861 gcgtccgggc gatgttcgac gagcacggca acacgctcag cgaggctggt ccggctcgtc
     1921 cggttgtcgt cctcggtctg gcctcggtcc cgcgggccgg cgacaccttc ctggtggcac
     1981 cggacgagcg gaccgcgcgt cagatcgccg agaagcgcga gtcggcggaa cgtgcggcac
     2041 tcctggcgaa gcgtcgcaag cgcatcagcc tcgaggactt cacccaggcg ctccagctgg
     2101 gcaaggtcga gaccctcaac ctggtcctca agggcgacgt gtcgggtgcc gtcgaggcac
     2161 tcgaggacgc gctgctcaag atcgacgtcg gcgaagaggt cgagctgcga gtcatccacc
     2221 gtggtgtggg tgccatcacg cagaacgatg tcaacctcgc cacggtcgac aacgcgatca
     2281 tcatcggctt caacgtgaag ttcggggagc gcgtcgagga cctcgcag
//
LOCUS       DASRKH010000423         3446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_46720, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000423 DASRKH010000000
VERSION     DASRKH010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46720"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1706
                     /locus_tag="VFC06_06775"
     CDS             <1..1706
                     /locus_tag="VFC06_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="maltotransferase domain-containing protein"
                     /protein_id="HZL81620.1"
                     /translation="DPVGSWVHAAEVKIHAGVDVKLMLDEGVDVLTRARDTVRRGAAA
                     RSLLTKAMATLTDGTLDPEKRLEAALSDKVRETLVKAPLREWVTTSAEYPLLIQREKA
                     LVSAWYEIFPRSEGAKHNNKTGEWTSGTFATAAKRLPAIADMGFDVVYLTPIHPIGTT
                     HRKGRNNALRAEPGDPGSPYAIGAEDGGHDAIHPDLGTMRSFKAFVKRAREVDLEVAL
                     DIALNASPNHPWLTEHPEWFTTRLDGTIAYAENPPKKYQDIYPFNFENDPEGSYQAWR
                     DIFQVWIDAGVTLFRIDNPHTKPLTFWERLLSDIADSRPDVLFLAEAFTRPAMMHTLA
                     KVGFHQSYTYFTWRQNAEEMGEYLEELAGESSFYMRPNFWPTTHDILTPDMQRGGAPY
                     WKMRAALAATGAPSYGIYTGYEFVESVPRPGVEEQIDNEKYEYKPRDWSKADEYGIAS
                     LLTTLNLLRSKHVALRRLRGLTVQRTTNPNILCFTRHVPANESPKGREDTVVVVASFD
                     PHAVNEAIVTLDMSTIGKQDDDPIPVLDAVTGAKYTWGREFFVRLDPSQTLVHVATVR
                     S"
     gene            1725..>3446
                     /gene="glgB"
                     /locus_tag="VFC06_06780"
     CDS             1725..>3446
                     /gene="glgB"
                     /locus_tag="VFC06_06780"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HZL81621.1"
                     /translation="MARDIDPTALADILAGTHHEPHAILGPHVHSGVVVVRVLRPFAK
                     SIAIETLDGKVPAKHVHDALWQAELPGVEIPDYRVHVTYDKEEVVTDDPYRFLPTLGE
                     LDLHLIREGRHERLWEALGAHVKSYPSVLGEVHGASFNVWAPNARAVRVVGEFNHWQG
                     VGHSMRSLGSSGVWELFIPGVGDGTHYKYEILGKDGHWKQQADPMARRTEVPPHTASV
                     VTASDYSWNDEAWLDNRTKHSAHDSAMSVYEVHLGSWKQGLNYRDLADDLIPYVRDMG
                     FTHLELMPVMEHPFGGSWGYQVTSYYAPTSRFGGPDDLRYFIDRAHQEGLGVLLDWVP
                     GHFPRDAWALGRFDGTPLYEHPDPLRGEQPDWGTFIFDFGRAEVRNFLVANALYWLQE
                     FHADGLRVDAVASMLYLDYSREAGQWRPNVHGGRENLDAIAFLQEANATAYKNVPGIV
                     MVAEESTAWPGVTAPTSEGGLGFGLKWNMGWMNDTLRYLAEEPVNRSYHHHTVTFSLI
                     YSFSEHFMLPLSHDEVVHGKGSLYTRMPGDHWQKIAGLRSLLAYQWAHPGKKLIFMGD
                     EFGQTAEW"
BASE COUNT          650 a         1191 c         1046 g          559 t
ORIGIN      
        1 ccgaccccgt gggctcgtgg gtgcacgctg cggaagtgaa gattcacgcg ggcgtcgatg
       61 tgaagctgat gctcgacgaa ggcgtcgatg tcctcacccg cgcccgggac accgtgcgca
      121 ggggtgcggc agcccgatcg cttctcacta aggctatggc cacactcacc gacgggacac
      181 tggaccccga gaagcgactt gaagcggccc tgtcagacaa ggtccgggag acgctcgtca
      241 aggcgcccct gcgcgagtgg gtgaccacat cggcggagta tccgctcctc attcagcgcg
      301 agaaggcgct ggtatcggcc tggtacgaga tcttccctag gtccgaagga gcgaagcaca
      361 acaacaagac gggcgagtgg acttcgggaa ccttcgccac cgcggccaag cggctgcctg
      421 cgatcgcgga catgggcttc gacgtcgtct atctcactcc catccacccc attggcacca
      481 cacaccgcaa gggacgcaac aacgccctgc gggcggagcc aggcgatccc ggaagcccgt
      541 acgcgatcgg cgccgaagac gggggtcacg acgcgattca ccccgacctg gggacgatgc
      601 gctcgttcaa ggccttcgtc aagcgcgcgc gcgaggtcga ccttgaggtc gcgctcgaca
      661 tcgccctcaa cgcctccccc aaccacccgt ggctgacgga acaccccgaa tggttcacca
      721 cgcggctcga cggcacgatc gcctatgcgg agaaccctcc caagaagtac caggacattt
      781 acccgttcaa cttcgagaac gaccccgagg gctcctacca ggcgtggcga gatatcttcc
      841 aggtgtggat cgacgccggg gtgaccctgt tccgcatcga taacccgcac accaagccgc
      901 tgaccttctg ggagcgactg ctgtcggaca tcgccgattc ccgtcccgac gtcctcttcc
      961 tggccgaggc cttcacgcgc ccggccatga tgcacacgct cgcgaaggtc ggcttccacc
     1021 agagctacac ctacttcacg tggcgccaga acgccgagga aatgggcgag tacctcgagg
     1081 aacttgcggg cgaatcgtcg ttctacatgc gccccaactt ctggccgacg acccacgaca
     1141 tcctgacccc cgacatgcag cgcggcggag caccgtactg gaagatgcgc gcggcgcttg
     1201 cggcgacggg cgccccctcc tacgggatct acaccggata cgagttcgtg gagtccgtgc
     1261 cccggcccgg agtcgaggaa cagatcgaca acgagaagta cgagtacaag ccgcgcgatt
     1321 ggtcgaaggc ggacgagtac ggcatcgcgt cgctgctcac gacgctcaac ttgttgaggt
     1381 cgaagcatgt cgcgttgcgc aggctgcgcg gcctcaccgt ccaacgcacc accaacccga
     1441 acatcctgtg cttcacgcgc cacgtgcccg ccaacgagtc ccccaaggga cgcgaagaca
     1501 ccgtcgtggt cgtggcatcg ttcgacccgc acgcggtcaa cgaggcgatc gtgaccctcg
     1561 acatgagcac catcggcaag caagacgacg accccatccc cgtcctggac gctgtgaccg
     1621 gagccaagta cacctggggg cgcgagttct ttgtgcgcct cgacccctcg cagacgctgg
     1681 tgcatgtggc cacggtgcgt tcatgagcac actggaggaa acccgtggca cgtgacattg
     1741 acccgactgc gttggcagac atcctcgctg gcacccacca cgagccgcac gcgattctcg
     1801 gaccacacgt ccacagcggc gtggtcgtgg tcagggtgtt gcgccccttc gccaagtcga
     1861 tcgcgatcga aaccctcgac ggcaaggtcc ccgccaagca cgtccacgac gcgctgtggc
     1921 aggccgaact cccgggtgtc gagattcccg actaccgggt gcacgtcacc tacgacaaag
     1981 aggaagttgt caccgacgac ccctaccgct ttctcccgac gcttggcgaa ctagaccttc
     2041 acctcatcag ggaaggacgc cacgagcggc tgtgggaagc gctcggagcc cacgtgaaga
     2101 gttacccctc cgtgctgggc gaggtccacg gcgcctcctt caacgtgtgg gcgcccaatg
     2161 cccgggccgt gcgcgtcgtc ggcgagttca accattggca aggcgtgggt cactccatgc
     2221 gatctctcgg ctcgtcgggc gtgtgggagt tgttcatccc cggcgtggga gacggcaccc
     2281 actacaagta cgagattctc ggcaaggacg gccactggaa gcaacaggcc gacccgatgg
     2341 cgcggcgcac cgaggtaccg cctcacactg cgtcagtcgt cacggcaagc gattactcct
     2401 ggaacgacga agcgtggttg gacaaccgca ccaagcactc cgcccacgac tccgcgatgt
     2461 cggtgtacga ggtccacctc gggtcgtgga agcagggtct caactatcgc gacctcgccg
     2521 acgatctgat cccgtacgtg cgcgacatgg gcttcaccca cctcgaattg atgcccgtca
     2581 tggagcaccc cttcggcggc tcgtgggggt accaggtgac gtcgtactac gcacccacct
     2641 cccgcttcgg cggccccgac gacttgcgtt acttcatcga ccgcgcccac caggagggcc
     2701 ttggcgtgct gctggactgg gttcccgggc acttccccag ggacgcgtgg gctctcggcc
     2761 gattcgacgg cactcccctg tacgagcacc ccgacccgct gcgcggcgag cagccggact
     2821 ggggcacctt tatcttcgac tttggccggg cagaggtgcg caacttcctc gtcgccaatg
     2881 cgttgtattg gcttcaggaa ttccacgccg acggcctgcg cgtggacgcc gtcgcctcca
     2941 tgctgtacct cgactactcg cgcgaagccg gccagtggcg gcccaacgtg cacggcggcc
     3001 gcgaaaacct cgacgcgatc gccttcctgc aggaggccaa cgcgacggct tacaagaacg
     3061 tgcccggaat cgtcatggtc gccgaggagt caaccgcgtg gccgggcgtc accgctccca
     3121 cgtcggaggg cggtcttggc ttcgggctca agtggaacat ggggtggatg aacgacaccc
     3181 tgcgctacct ggcggaggag cccgtcaacc gctcatacca ccaccacacc gtgacgtttt
     3241 cgctgatcta ctcattctcc gagcacttca tgttgccgct cagccacgac gaggtcgtcc
     3301 acggcaaggg ctccctgtac acccgcatgc ccggagacca ttggcagaag atcgcgggct
     3361 tgcgttcgct gctcgcctac cagtgggctc accccggcaa gaagctcatc ttcatgggcg
     3421 acgaattcgg ccagacggcc gaatgg
//
LOCUS       DASRKH010000424         3331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_16148, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000424 DASRKH010000000
VERSION     DASRKH010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16148"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="VFC06_06785"
     CDS             complement(<1..1080)
                     /locus_tag="VFC06_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81622.1"
                     /translation="MRNGMFAPWEGWRKALGGVAAAFMVLLVIAFSIPLGIVAVGAMF
                     LAAQIWKSPMDSPNYDRMQYTMMTRSYRVALFFVTVKQQAAVLFSPVILLGAGPPRED
                     PDHDLQPGFWPPFRLSSWWSMLGALALAPLEILISPMRMSVIGGSLPWFIATALAIPG
                     WYAFFQVLADARRLAPDKDAGALGDPPPATMLNKIDDHIELHPSLIRSATFGAIPVVI
                     VIILWITVKWPWWVTLIAVILSFTFTAAILWSRNLMTEYRSAWVEREALAENWQSIWM
                     SLLPVRAGSPLFSNEFDLPSVDEFEAELARWHREMDESDDLDASPPEYEVLVKCANFR
                     YAPGQTYETVAGMTEGLRGALNAGLV"
     gene            complement(1206..1685)
                     /locus_tag="VFC06_06790"
     CDS             complement(1206..1685)
                     /locus_tag="VFC06_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81623.1"
                     /translation="MNVLETAARDRVGSMTALARQTGISERLLAAIGAGEVEPGTAHV
                     RRIRQVLGQDLHVDAFPHCAPKVDSLMNVSETKVLAVRDEHLVRPASVIKDDMIRLVS
                     VRDDGREKVEGWVKAIEDARWIRGDLVAFALKDGFVVECHPSQTVTVARRRPPAEVA"
     gene            complement(1682..3112)
                     /locus_tag="VFC06_06795"
     CDS             complement(1682..3112)
                     /locus_tag="VFC06_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81624.1"
                     /translation="MSVRELPSRVSPLAHTPVPEAPAGVPAAQYSPDKEDFGLPSQRS
                     IWEREFLVTRRSQTMGVSRRENQGARRTNIGHPPVRLVDRIDPFWEITWDDMIGGADW
                     RRASTTKHRLTTDVRGNVVVKSFEAFSPIAMSQQESYGENMMRMMALLFQFGALTSSQ
                     LCSFMDLSFRGLSFSLNRMFGLGLVERMTPAWVKVGPSSRMYHGSGDIWRVNLRSETV
                     TTWLDGLSNTDYALMTGGRDAGYMLHNGTFVSEYALRHNLAVAELCLRGLETSPGLAG
                     AWGEPFTEGNKFLSEMLRGSMDVRYVRGDAALVGKDGSVVIVELTAGQDSRDDKHGAT
                     LAARAAAWAAIAKMSDVPMSLVVVSIGGYAASRRIAKHLRENTENELAQYFADVGDRK
                     RALDTVHMSSIQSWWPSAGAISRKFLTLEAFSPFTGEYRELLPADAEWDTGSPVVVNT
                     VAALHTPPWSLHPVMPIVNNEEEPTA"
     gene            complement(3109..>3331)
                     /locus_tag="VFC06_06800"
     CDS             complement(3109..>3331)
                     /locus_tag="VFC06_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81625.1"
                     /translation="VNADGAITPSNIKSLSNGVCFATLPRNNSVTPPTLLRVPYAPEW
                     NKILFAPENGDTNDAVADYAEFTARKVAS"
BASE COUNT          610 a         1070 c         1092 g          559 t
ORIGIN      
        1 gacaaggcct gcgttcaatg ctccgcgtag cccctctgtc attccggcca cagtctcgta
       61 ggtctggccg ggtgcgtacc ggaagttggc gcacttgacg agcacctcgt actcgggcgg
      121 cgaggcgtca aggtcgtcag actcgtccat ctcgcggtgc cagcgcgcca gttccgcctc
      181 gaactcgtcg acgcttggca ggtcgaactc gttgctgaac agcggggatc cggccctcac
      241 tggaaggagg ctcatccaga tgctctgcca gttctcggcc agggcttcac gctccaccca
      301 ggcggagcgg tactcggtca tcaggttgcg tgaccacagg atcgcagcgg tgaaggtgaa
      361 cgacaggatg acggcgatga gcgtcaccca ccagggccac ttcacggtga tccacaggat
      421 gatcacgatg acgacgggga ttgccccgaa ggtcgccgag cgaatcagcg acggatgcag
      481 ttcgatgtgg tcgtcgatct tgttcagcat ggtcgccggt ggcgggtcgc ccagtgcgcc
      541 agcatccttg tcgggggcga gcctgcgtgc gtcggccagc acctggaaga acgcgtacca
      601 gcctgggatc gcaagggctg tcgcgatgaa ccacggcagc gacccaccga tgacggacat
      661 gcgcatcggg ctgatcagga tttccagcgg agcgagggcg agagcgccca gcatgctcca
      721 ccaggatgaa agccggaacg gcggccagaa gcctggctgc aagtcgtggt cggggtcctc
      781 gcggggcggg ccggcgccga ggaggatgac cggagagaac agcacggccg cctgctgctt
      841 cacggtgacg aagaacaggg cgacacggta ggaccgggtc atcatcgtgt actgcatgcg
      901 gtcgtagttc ggcgagtcca tcggactctt ccagatttgc gcggcgagga acatcgcacc
      961 aacagcgacg atgcccagtg ggatggagaa ggcgatgacg agtagcacca tgaaggcggc
     1021 ggccacgcca cccaaagcct tgcgccaccc ctcccacgga gcgaacatgc cgtttctcac
     1081 tacaccactc ccctacgtct aagacctacc acagcataac ggccacagag aggcccatat
     1141 ctgcccacag cgggccacag aggcgcgctg tggcatgccg acgagggacc acacttttga
     1201 ggggcttagg cgacctctgc cggtgggcga cgtcgggcga cagtgaccgt ctgcgacggg
     1261 tggcattcga ccacgaagcc gtccttgagg gcgaacgcga ccaggtcgcc gcggatccag
     1321 cgcgcgtcct ctatggcctt cacccagcct tcgaccttct cccgcccgtc atcacgtacc
     1381 gagacgagtc ggatcatgtc gtccttgatc acggaggcgg ggcgcacgag gtgttcgtcg
     1441 cgtacagcca gcaccttcgt ctccgacaca ttcatgagcg agtcgacctt cggcgcacag
     1501 tgcgggaagg cgtcgacgtg gaggtcctgc ccgagcacct gacggatccg gcgcacatgg
     1561 gccgtgccgg gctcaacctc gcctgcacct atggccgcga gcagccgctc gctaatgccc
     1621 gtctgcctcg ccagtgccgt catggaaccg acgcggtcgc gtgcagcagt ttcgaggacg
     1681 ttcacgccgt gggctcctct tcgttgttga cgatgggcat cacggggtgc agcgaccacg
     1741 gcggcgtgtg caacgcggcg accgtgttga cgacgacggg cgaaccggtg tcccattcgg
     1801 cgtcagccgg cagcagttcg cggtactccc ctgtgaacgg cgagaacgcc tccagcgtga
     1861 ggaacttgcg gctgatggcc ccagcggacg gccaccaact ttggatggat gacatgtgca
     1921 cggtgtcgag cgcccgtttg cgatcgccga cgtccgcgaa gtattgggcg agttcgttct
     1981 cggtgttctc gcgcaggtgc ttggcaatcc gtcgcgaggc cgcgtaaccg ccgattgaga
     2041 cgacgacgag cgacatgggg acgtccgaca tcttagcgat cgccgcccag gcagcggcgc
     2101 gcgccgccaa ggtggcacca tgcttgtcgt cgcgagagtc ctgcccggcg gtcagttcga
     2161 cgatcacgac ggacccgtcc ttgccgacga gcgctgcgtc accgcgcaca tacctgacgt
     2221 ccattgaccc gcgaagcatc tcggagagga acttgttgcc ctcggtgaag ggttcacccc
     2281 acgcaccggc caagccgggg ctcgtttcga gcccgcggag gcacagttcg gcaaccgcga
     2341 ggttgtgccg cagcgcgtac tcggacacga acgtgccgtt gtggagcatg tagcccgcgt
     2401 cacggccgcc tgtcatcagg gcgtagtcgg tgttcgacag accgtcgagc cacgtcgtca
     2461 ccgtttcgga gcgcaggttc acgcgccaga tgtcaccgga cccgtggtac atcctcgaac
     2521 tggggccaac cttcacccaa gcgggcgtca tgcgctccac caggccgagc ccgaacatcc
     2581 ggttcaggga gaacgagagc cccctgaagg acaagtccat gaatgagcac agttggctgg
     2641 acgtcaaagc gccgaactgg aacagcaacg ccatcatccg catcatgttc tcgccgtacg
     2701 actcctgctg gctcatggcg attggagaga acgcctcaaa cgacttcaca acgacattgc
     2761 cgcgcacgtc cgtcgtcaga cggtgcttcg tggtggacgc cctgcgccag tcggcaccac
     2821 caatcatgtc atcccaggtg atctcccaga acgggtcgat gcggtcaacg aggcggaccg
     2881 gtgggtgacc aatgttcgtg cggcgcgccc cttggttttc gcgacgcgac acgcccatcg
     2941 tctgcgagcg acgagtcacg aggaactccc gctcccagat cgaacgctgg ctcggcagac
     3001 cgaagtcttc cttgtcgggc gagtattgcg ctgcggggac gccggcgggc gcctccggca
     3061 caggagtgtg tgcgagcggg gaaacgcgtg acgggagttc gcggacactc atgacgccac
     3121 cttgcgggcg gtgaactccg cgtagtcggc caccgcgtcg ttcgtgtcgc cgttctccgg
     3181 ggcgaacaga atcttgttcc actccggggc gtacggcaca cgcagcaacg tgggcggggt
     3241 caccgagttg ttgcgcggca acgtcgcgaa gcacacgccg ttggacagcg acttgatgtt
     3301 gctgggcgtg atcgcgccgt cggcgttcac g
//
LOCUS       DASRKH010000425         2364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_20536, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000425 DASRKH010000000
VERSION     DASRKH010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20536"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VFC06_06805"
     CDS             complement(<1..525)
                     /locus_tag="VFC06_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZL81626.1"
                     /translation="MTRARGEKARGVQELSEQGLDRYQSALMGTFGRPMRVLTKGEGS
                     YVWDADGNRYLDLLGGIAVNALGHADPEWVAAIAAQAATLSHVSNFFATEPQIALAER
                     LLDICGAPVGSRVFLTNSGTEANEAAFKMSRRTGRTRILALTESFHGRTTGALTLTYK
                     EAYRAPFAPLAPGVD"
     gene            complement(522..1439)
                     /gene="argB"
                     /locus_tag="VFC06_06810"
     CDS             complement(522..1439)
                     /gene="argB"
                     /locus_tag="VFC06_06810"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HZL81627.1"
                     /translation="MSDLIDTPDLNPQQKVSVLIDAMPWIEQFKGAIVVIKYGGNAMV
                     DDVLKRAFAQDIVHLRLLGLKPVVVHGGGPQITDMLSRLGIESEFKGGLRVTTPEAMD
                     VVRMVLTGQVSRELVGLINDHGPLGVGLSGEDAGLLQAKRRHALVDGKLVDVGQVGDV
                     VKVNPAAIQDIISAGRIPVVSTVAPDMDDPTTVLNVNADTAAASIAVALRARKLIVLT
                     DVEGLYANWPDRTSLVRRIHATDVEKLLPSLMSGMVPKMEACLRAVRGGVSQAHIIDG
                     REPHSILTEIFTSSGVGTMVRPDDVELWG"
     gene            complement(1436..>2364)
                     /locus_tag="VFC06_06815"
     CDS             complement(1436..>2364)
                     /locus_tag="VFC06_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HZL81628.1"
                     /translation="ADTHTTAERVASALQAVGRDVSAGDVAVCSTGLIGVRLPMDKLL
                     GGVDAAVGELAADGGDAAAHAIMTTDSVRKTAVAERDGWSIGSMAKGAGMLAPGMATM
                     LCVITTDADIDPAQADAALKEAVRTTLNRLDADGCMSTNDTVVLMASGASGVSPEPAA
                     FQEALTAVCHDLAQQLLTDAEGAEHDVAIKVINATTEDAAEAVARAVTRSNLFKAAVF
                     GRDPNWGRIISAAGTVPEEIAPFDANLLDVYFNGVQVCKAAGVGESRDLVDLAPRAVD
                     VVIDLHAGDATATVWTNDLTHDYVHENSAYSS"
BASE COUNT          355 a          791 c          814 g          404 t
ORIGIN      
        1 gtcgacgccc ggtgccagcg gcgcgaaggg cgcgcggtac gcctccttgt acgtcagcgt
       61 gagcgcgccc gtggtccggc cgtggaacga ttcggtgagc gcgaggatcc gggtgcgtcc
      121 ggtcctgcgc gacatcttga aggccgcctc gttcgcctcg gtgccggagt tggtcaggaa
      181 cacgcgggag cctacggggg ctccgcagat gtccagcagc cgctccgcca aggcgatctg
      241 tggctccgtc gcaaagaagt tggagacgtg ggacaaggtg gctgcctggg ctgcgatcgc
      301 cgcgacccat tcggggtcgg cgtggcccaa ggcgttgacc gcgattcctc cgaggaggtc
      361 caggtagcga ttgccgtcgg cgtcccacac gtacgagcct tcgcccttgg tgagtacgcg
      421 catcgggcgc ccgaaggtcc ccatcaacgc cgactggtag cggtcgaggc cctgctcgct
      481 gagttcctgg acaccgcgtg ccttttcacc gcgtgccctg gtcatcccca cagctccacg
      541 tcgtcgggcc gtaccatcgt gcccacaccg gacgaggtga agatctcggt gaggatcgag
      601 tgcggttcgc ggccgtcaat gatgtgcgcc tggctcacgc cgccacgcac ggcccgcagg
      661 caagcctcca tcttgggaac catgccggac atgaggctgg gaagcagctt ctccacgtcg
      721 gtcgcgtgga tgcggcgtac gagtgaagtg cggtcgggcc agttcgcgta caggccctcc
      781 acgtcggtca ggacgatgag cttgcgggct cgcagcgcca cggcaatcga cgcggcggcc
      841 gtgtcggcgt tgacgttgag caccgtggtg gggtcgtcca tgtcgggcgc gaccgtgctg
      901 accacgggaa tgcggcctgc agagatgatg tcctggatgg ccgcgggatt gaccttgacc
      961 acgtcgccga cctggcccac gtcgaccaac ttgccgtcga ccaaggcgtg gcggcgcttg
     1021 gcttgcagga ggcccgcgtc ctcaccggac agtcccacgc cgagcggacc gtggtcgttg
     1081 atgagcccca cgagttcgcg cgacacctgg ccggtgagca ccatgcgcac cacgtccatc
     1141 gcctcgggcg tcgtgacgcg caggccgccc ttgaactcgg actcgatgcc gagccgactc
     1201 agcatgtcgg tgatctgcgg ccccccgccg tgcaccacca cgggcttgag acccaagagc
     1261 cgcaagtgca cgatgtcttg cgcgaaggcg cgcttcagca cgtcgtcgac catggcgttg
     1321 ccgccgtact tgatgacgac gatggcgccc ttgaactgct caatccaggg catcgcgtcg
     1381 atgaggacgc tgaccttctg ctgagggttg aggtcgggtg tatctatcag atcgctcatg
     1441 agctgtacgc actgttctcg tggacgtagt cgtgggtgag gtcgttcgtc cagacggtgg
     1501 cggtggcatc gcccgcgtgc aggtcgatca ccacgtcgac agcgcgcggg gccaggtcga
     1561 cgagatcgcg tgattcgccc actcccgccg ccttgcacac ctgcacgccg ttgaagtaga
     1621 cgtcgaggag attcgcgtca aacggcgcaa tctcttcggg caccgtgccc gccgcggaga
     1681 tgatgcggcc ccagttgggg tcgcgaccaa agactgcggc cttgaagagg ttcgagcggg
     1741 tcaccgcgcg ggcgacggct tccgccgcgt cctccgtggt ggcgttgatg accttgatcg
     1801 cgacgtcgtg ctccgcgccc tctgcgtccg tcagcaactg ctgggcgaga tcgtgacaca
     1861 cggcggtgag cgcctcctgg aatgcggcag gttccgggct cacgcccgac gcgccggagg
     1921 ccatcagcac caccgtgtcg ttggtggaca tgcacccgtc ggcgtcgagg cgattcaggg
     1981 tggtgcgcac cgcttccttg agggcggcgt cggcctgcgc cgggtcgatg tcggcgtcgg
     2041 tggtgatgac gcacagcatc gtggccattc cgggcgcgag catgcccgcg cccttcgcca
     2101 tcgagccgat cgaccagcca tcgcgctccg cgaccgcggt cttgcgcacc gagtcggtgg
     2161 tcatgatggc gtgcgcggcc gcgtcgccgc cgtcggctgc caactctccg acggccgcat
     2221 cgacgccgcc gagcaacttg tccatcggca accgcactcc gatgagtccg gtcgagcaga
     2281 cggccacatc ccctgccgag acatcgcggc ccacggcctg cagcgccgac gccacgcgct
     2341 cggcggtggt gtgagtgtcg gcga
//
LOCUS       DASRKH010000426         2156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_9778, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000426 DASRKH010000000
VERSION     DASRKH010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9778"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VFC06_06820"
     CDS             <1..556
                     /locus_tag="VFC06_06820"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HZL81629.1"
                     /translation="QQDSIRRAMTLVVGVDGRVLHFVEWKRAISMVTARQAYVIETVK
                     RRTDTGELEDALVRSPSVRIPLPLIVGLLPHVSVPAVAFVHAKSEKAGRIAILRRDKK
                     VCTYCGERGLTIDHIFPKSRGGRSTWQNLVAACGECNNRKADRTPEEAGLVMLFDPRV
                     FEGGMADLQAEVWRHMDAACGVVA"
     gene            696..1484
                     /locus_tag="VFC06_06825"
     CDS             696..1484
                     /locus_tag="VFC06_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81630.1"
                     /translation="MTVVDLATTQAYIHPHHGVALMDGPTVERSVKGHSVLMQPLAAA
                     QAQSWAALLGLPGGAILANAEVHSLVPVSSLDSETCSWARCPRVALEDIVRHLDVEPV
                     DLWIDQIDVPAPVKMLNVCQSRMVMVGRGDDPTAELSELVRQVADYLTVWHLVGLYGV
                     LDHVVVRRGLAETFEEAFTHGGPFWSRCIAQTYTMRVSGRDLIALLGRVVSVIALSSD
                     LQPEQVASTLELAISNRVRARILELRDHAAGCRASVANVDPTKW"
     gene            1517..1777
                     /locus_tag="VFC06_06830"
     CDS             1517..1777
                     /locus_tag="VFC06_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81631.1"
                     /translation="MSESPFGGIGETASSTPDQPAQTPQSNVRAHLRAIIGAVLVLVG
                     FAWKFFAGSAAGADSSTLVDAAWLVPIAVGGALLVWSMRRRT"
     gene            1819..2133
                     /locus_tag="VFC06_06835"
     CDS             1819..2133
                     /locus_tag="VFC06_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81632.1"
                     /translation="MTNATLGEFNATLGEFLAWSGKPAREGSALERAAIALVEELAGQ
                     FLKERNDAMIALDADAPFEPLHVIDGGLTQKRTTEEIQCMADHPSARGHGAQRLQALP
                     VR"
BASE COUNT          380 a          671 c          703 g          402 t
ORIGIN      
        1 ccagcaggac agcattcgtc gcgcaatgac cctcgtggtc ggcgttgacg gccgcgtctt
       61 gcacttcgtc gagtggaagc gtgcgatctc gatggtgacg gccaggcagg cgtacgtcat
      121 cgagaccgtc aagaggcgga cagacaccgg ggagttggaa gacgccctcg tgcggtcgcc
      181 atctgtgcgg atccccttgc cgctgatcgt gggactgctg ccgcacgtga gcgtcccggc
      241 cgtcgcgttc gttcatgcca agtccgagaa ggcgggccga atcgcgatcc ttcggcgaga
      301 caagaaggtg tgcacgtact gcggggagcg aggtctgacc atcgaccaca tcttcccgaa
      361 gtcgcgtggc ggccgcagca cgtggcagaa cctcgtggcc gcgtgcggtg agtgcaacaa
      421 ccgcaaggcg gaccgcactc cggaagaggc cggcctggtc atgctgttcg accctcgcgt
      481 gttcgagggc gggatggccg acctgcaggc cgaggtgtgg cgccacatgg acgccgcttg
      541 cggcgttgtt gcctgacaga acactcatcc accgtggcgg caggggctcg acatccgtac
      601 gtggtgccgg gcccctgctg cgtcttgggc ccaggcgatg cccgaaaagt ggggtagtct
      661 ttgcatagtc caccacactc catcggagga tgacaatgac cgtcgtagac ctcgccacga
      721 cacaggccta catccacccg catcacgggg tggccctgat ggacggtccg actgtcgaac
      781 ggagtgtcaa ggggcactcg gtgctgatgc agccgctcgc cgcggcgcag gcgcagtcgt
      841 gggcggcgct cctcggtctg ccgggcggag cgatcttggc aaacgccgag gtgcactccc
      901 tcgtacctgt ctcctcactt gactcagaaa catgctcctg ggcgcggtgc ccccgcgtgg
      961 cgctggaaga catcgtccgt cacctggacg tggagcccgt ggatctgtgg atcgaccaga
     1021 tcgacgtacc ggccccggtg aagatgctca acgtttgtca gtcccgaatg gtgatggtcg
     1081 gcagaggcga cgacccgact gctgaactct ccgagttggt acgccaggtc gccgactact
     1141 tgaccgtgtg gcacctcgtg gggctgtatg gcgtccttga tcacgttgtg gtcaggagag
     1201 gcctcgcaga gaccttcgag gaagccttca ctcatggcgg gccattttgg agcaggtgca
     1261 tcgctcagac gtacacgatg cgtgtctctg gtcgcgacct gatcgcccta ctcggacgcg
     1321 tcgtctccgt gattgcgctc agtagcgacc tacagcccga gcaggtcgct tcgacgctcg
     1381 aactcgccat ctccaaccgg gtgcgggctc gcatcttgga acttcgcgac catgcggccg
     1441 ggtgccgcgc cagcgtagca aatgtcgacc caaccaagtg gtagttttgt cctgacatcc
     1501 tgaaaggaag tgcaccatgt ccgagagccc cttcggtgga atcggcgaga ccgcaagttc
     1561 cacccccgac cagcccgcac agaccccgca gagcaatgtt cgtgcgcacc tgcgggcgat
     1621 catcggcgcg gtccttgtac tcgtgggctt cgcctggaag ttcttcgctg gcagcgccgc
     1681 gggggccgac agcagcactc tggtcgacgc cgcatggctc gtcccgattg cggttggtgg
     1741 tgcactgctc gtatggagca tgaggcgtcg cacctaagcg tggtcttctc gccctttgag
     1801 gtgtggtagt tttgactcat gactaacgca actcttggag aatttaacgc taccctcggc
     1861 gagtttcttg catggtccgg caagccggcc cgcgaaggca gtgcacttga acgtgcggct
     1921 atcgcgctcg tggaagaact cgcagggcag ttcctgaaag aacgaaacga cgcgatgatc
     1981 gctctggacg cagatgcgcc cttcgagccc ttgcacgtca ttgatggcgg cctcacgcag
     2041 aagcggacca ccgaagagat ccagtgcatg gccgaccacc cgagtgcccg cgggcatggg
     2101 gcgcaacggt tgcaggcgct accagttcgg tagggcaggc ccgcggatac tctgta
//
LOCUS       DASRKH010000427         2226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_20582, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000427 DASRKH010000000
VERSION     DASRKH010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20582"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VFC06_06840"
     CDS             complement(<1..212)
                     /locus_tag="VFC06_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HZL81633.1"
                     /translation="MSDETLPAFASLNVGDQVSARDVEITRDMLVRYAAASGDFNPIH
                     YNDAFAQQVGLPGVIAHGMLTMGIGAS"
     gene            complement(209..658)
                     /locus_tag="VFC06_06845"
     CDS             complement(209..658)
                     /locus_tag="VFC06_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017488955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HZL81634.1"
                     /translation="MPVNPETQGRTYGPFEPYEVTRGAIRAFADAVDARGGAHFYTEV
                     AHAEGYADVVAPATFAAIIAQKAEFHVVTDPEVGVDFSKVVHAEERFTHHRPIVAGDV
                     IRAGITIESITQRAGISMVTSRAELTDADGVPVTTVLSTLAVREDQS"
     gene            complement(781..945)
                     /gene="rpmG"
                     /locus_tag="VFC06_06850"
     CDS             complement(781..945)
                     /gene="rpmG"
                     /locus_tag="VFC06_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012085006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HZL81635.1"
                     /translation="MAKNDVRPKITLACTECKNRNYITRKNRRNTPDRVELAKFCRKC
                     GKHQPHRETR"
     gene            complement(1011..1084)
                     /locus_tag="VFC06_06855"
     tRNA            complement(1011..1084)
                     /locus_tag="VFC06_06855"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1048..1050),aa:Met,seq:cat)
     gene            complement(1133..1208)
                     /locus_tag="VFC06_06860"
     tRNA            complement(1133..1208)
                     /locus_tag="VFC06_06860"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1173..1175),aa:Thr,seq:ggt)
     gene            1269..>2226
                     /locus_tag="VFC06_06865"
     CDS             1269..>2226
                     /locus_tag="VFC06_06865"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HZL81636.1"
                     /translation="MHTSYLMVTVSLVTRNVSAAQTRRVFLNAQGLARKRPARAARER
                     DFATYLSTQGVLQLDTVNVLARAHYLPLYSRFGVFDLQQADAFLWGDARGHSAHAFEH
                     WGHEASVMPRELLPAMHHRMVDGNSWQSRYRERLDRERPGLIDAARELAERGGPVVGA
                     DVSHLAPRVRRKGPWWDNDHAKDALEYLFITGKVAASRGRHFARTYDATHRAWGLPPA
                     DAVAADGSHPWGLPAADARQQLFDRALAACGIGTVADLCDHFRLPLKPGMRAPDAAGG
                     EAWAASAVERGLAEWVEVEGWKTPALMAVGEAVKVALAVRVVD"
BASE COUNT          359 a          734 c          762 g          371 t
ORIGIN      
        1 gaggcgccga tgcccatcgt cagcatcccg tgggcgatca caccgggcag tcccacctgt
       61 tgggcgaagg cgtcgttgta gtggatcggg ttgaagtcgc ccgaggcggc ggcgtagcgc
      121 acgagcatgt cgcgcgtgat ctcgacgtcg cgcgccgata cctgatcgcc cacattgagg
      181 gacgcgaagg ccggcagggt ctcatcgctc atgactggtc ctcccgcacg gccaacgtcg
      241 acagcaccgt ggtgaccggg acgccgtcgg cgtccgtcag ttcggcgcgg ctggtcacca
      301 tggagatgcc cgcgcgctgc gtgatcgact cgatggtgat cccggcgcgg atcacgtcgc
      361 cggccacgat gggccggtgg tgcgtgaaac gctcttcggc gtggacgacc ttggagaagt
      421 cgacgcccac ctcggggtcg gtgaccacgt ggaactcggc cttctgggcg atgatggcgg
      481 cgaatgtcgc cggcgcaacc acgtccgcat acccctcggc gtgcgcgacc tcggtgtaga
      541 agtgcgcgcc tccacgagca tcgacggcat cggcgaaggc tcggatggcc ccacgagtca
      601 cctcgtatgg ctcaaagggg ccgtaggtgc ggccctgggt ttcgggattc acaggcacgt
      661 atcaacccta ccgaaaggga cgatgccggc aacccctacg gcgcgcggag aactcccgca
      721 ccggaaaacg agacgcccgc cgcggcacgg gccgggcggg cgtctacgac gtggtcgaga
      781 ctagcgagtc tcgcggtggg gctggtgctt gccacacttg cggcagaact tcgcgagctc
      841 cacgcggtca ggcgtgttgc ggcggttttt gcgcgtgatg tagttgcggt tcttgcactc
      901 tgtgcacgcg agcgtgatct tcggacgaac gtcattctta gccatggttc aaatccctgc
      961 cttgttgttc acgctcgcgc gtgcttgccc atccggatcg atggaacgtg tagcgagggc
     1021 gggactcgaa cccgcgaccc cacgattatg agtcgtgtgc tctaaccacc tgagctaccc
     1081 cgccgcacaa gtaccgactt accgtcgcca gaagcaatgg taggtcaatc cttggagccc
     1141 cgacagggaa tcgaaccctg gaccttttcc ttaccatgga aacgctctac caactgagct
     1201 atcggggcaa gccggactag cgtacatggc gcacgggtcc cacaggaaat cgtccccatc
     1261 ccccgaccgt gcacacgtca tacctgatgg ttacagtgtc gctcgtgacc cgtaacgtga
     1321 gcgccgccca gacccgacgc gtctttctga atgcccaggg gctcgcgcgc aagcgaccgg
     1381 cgcgggcggc gcgcgaacgc gactttgcga cgtatctgag cacccagggc gtactccagc
     1441 tcgacaccgt gaacgtgctc gcccgggccc actacctgcc gctctactcc cggttcgggg
     1501 tgttcgacct gcagcaggcc gacgcctttc tgtggggcga tgcccgcggc cactcggccc
     1561 acgccttcga gcattggggg cacgaggcct cggtcatgcc tcgggaactg ctccccgcga
     1621 tgcaccaccg catggtggac ggcaactcgt ggcagtcgcg ctatcgggag cggttggatc
     1681 gcgagcgccc cggattgatc gacgccgcca gggagttggc cgagagaggt ggcccggtgg
     1741 tgggcgccga cgtctcgcac ctggccccac gcgtgcgccg caagggcccg tggtgggaca
     1801 acgaccacgc caaggatgcg ctcgagtacc tgttcatcac gggcaaggtt gcggcgtcca
     1861 gggggcgcca ttttgcgcgc acgtacgacg cgacgcacag ggcgtgggga ctgcccccgg
     1921 ccgatgcggt ggcggccgac ggctcgcacc cgtgggggct gcctgcggcc gacgcgcgtc
     1981 agcaactctt tgaccgcgcg ctcgccgcgt gcggcatcgg cacggtggcg gatctttgcg
     2041 accacttccg cctgccgctc aagcccggca tgcgcgcgcc cgacgccgcc ggcggcgagg
     2101 cgtgggcggc gtccgcagtg gaacgcgggc tggccgagtg ggtcgaggtt gagggatgga
     2161 agactcccgc gctaatggcc gtcggcgagg ccgtcaaggt agcgcttgcc gtgcgagtcg
     2221 tagatg
//
LOCUS       DASRKH010000428         2302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_5570, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000428 DASRKH010000000
VERSION     DASRKH010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5570"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..1498
                     /gene="hemG"
                     /locus_tag="VFC06_06870"
     CDS             95..1498
                     /gene="hemG"
                     /locus_tag="VFC06_06870"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HZL81637.1"
                     /translation="MAAPVVVIGGGITGLGAAWELTEAGHRVLLVEAADRFGGKVHSE
                     TVDGFLIEHGPDSFVSYRPGAVQMAADLGMSDDVVPAVPPRLVHLRVHGRMEPIPAGM
                     GMVLPTRLIPFARTRILSWPQKLRATMDLVLPRVLGPQDMSIGALLRRRLGHGVVARF
                     ADPLVGGIYGATVDELSVDAVLPVLRTHEAQRRSLIVASVAQGRLAPRPASPDASPFR
                     SMVAGMGSLVVGLVKGLRERDADLRTGARAVGLEVAGTGAVVTLADGTRHEAAGVVFA
                     GGGACSAALLNDVAPAAAAALRAIPHGSTATVHLGFHADAFVTPPVGHGYLEAGPEPA
                     PITGITIASNKWPGRAPEGMVLIRAFVPGRVGAASQLPDDALLDTVSAYVSQVLGAHS
                     APFLRHLVRWQDAMPTYTVGHLDRVRAVADALTDTPQLRVAGSALHGVGVPDCLADGR
                     KVGGELAAVLIVQPTTP"
     gene            1511..>2302
                     /locus_tag="VFC06_06875"
     CDS             1511..>2302
                     /locus_tag="VFC06_06875"
                     /inference="COORDINATES: protein motif:HMM:NF012964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HZL81638.1"
                     /translation="MSASQSPMTTGETSGYDAVLLASFGGPEGPDEIMPFLERVTAGK
                     GIPRDRLEAVHQHYLRMGGRSPINDQNRTLLAALRAELNRRGVTIPLVLGNRNSPPFI
                     DEALRELDTAGCRRVLAFATSQYSSYSSCRQYRENLAEALSSTGLADRMQVAKVRPAF
                     DRAGFVETTVELLASAVSGRAREGRRVLFTTHSIPSADAAASGPGAEPGEVDLYSAQH
                     REVANAVIGGVAALASEKPLPWRLVYQSRSGSPRVPWLEPDINDAL"
BASE COUNT          321 a          832 c          788 g          361 t
ORIGIN      
        1 ggacggggga tcccctcgag tccgaccccc tgtgcccgta cgtgccgcgc gacacggacc
       61 gcaccgacgc gatgcccgcg gcgagcggcg cgtaatggcg gcaccggttg tcgtcattgg
      121 cggcggcatc accgggctcg gcgccgcatg ggagttgacg gaagctggac accgcgtgct
      181 cctcgtcgag gccgccgatc gttttggggg gaaggtgcac tcggagacgg tcgacggatt
      241 cctcatcgag cacggccctg actccttcgt cagttaccgg cccggtgcgg tacagatggc
      301 agcggacctc ggcatgtcgg acgacgtcgt ccccgctgtg ccgcctcgcc tggtgcacct
      361 gcgcgtgcac ggccgcatgg aacccatccc cgccgggatg gggatggttc tgcccacccg
      421 gctcatcccg ttcgccagga ctcggatcct cagctggccg cagaaactgc gcgcaaccat
      481 ggacctcgtg ctaccgcgag tgctcggtcc gcaggacatg tcgatcgggg ccctgctgcg
      541 gcgcaggctc ggacacggag tggtcgctcg cttcgccgac ccgctcgtcg gcgggatcta
      601 cggggcgaca gtcgacgagc tgagcgtcga cgccgtcctg cccgtgctgc gaacccatga
      661 ggcacagcgg cgcagcctga tcgtcgcgtc ggtggcgcag ggcaggctag ccccgcggcc
      721 cgcctccccc gacgcctcac cctttcggtc gatggtggcg ggcatgggct cgctggtggt
      781 tggcctcgtc aagggcctcc gggagcggga tgccgacctg cgaacgggcg cgcgcgccgt
      841 ggggctggag gtggccggga ccggtgctgt cgtcactctc gcggatggca cccgccacga
      901 ggccgctggg gtggtgttcg ctggcggggg cgcatgctcc gcagcgctgc tcaacgacgt
      961 cgcgcccgcg gcggccgcgg ccttgcgcgc catcccgcac ggttcgacgg caacagtcca
     1021 cctcggcttc cacgccgatg ccttcgtcac gccaccggtc ggccatggct acctcgaggc
     1081 aggtcccgag ccggcgccga tcaccggcat caccatcgcc tccaacaagt ggccgggccg
     1141 tgcgccagag gggatggtcc tcatccgtgc gttcgtcccg ggcagggttg gcgccgcatc
     1201 gcaacttccc gacgacgccc tgctcgacac cgtcagcgct tacgtctcgc aagtgctcgg
     1261 ggcgcactcc gcgccgttcc tgcgccacct ggtccgatgg caagatgcga tgcccaccta
     1321 taccgttggc cacctggacc gggtgcgcgc tgtcgccgac gcactcaccg acacccctca
     1381 gttgcgcgtc gccggttcgg cgctgcacgg ggtgggcgta ccagactgtc tggccgacgg
     1441 cagaaaggtc ggcggcgagc tggccgctgt tcttattgtc caacccacga ccccctgagg
     1501 aattaatctc atgtccgcta gccaaagtcc gatgaccacc ggcgagacca gcgggtacga
     1561 cgccgtcctt ctcgcctcgt tcggcggacc ggagggcccg gacgagatca tgccgttcct
     1621 cgagcgggtg accgccggga agggcatccc gcgcgaccgg ctcgaggccg ttcaccagca
     1681 ctacctgcga atgggtggcc gcagcccgat caacgaccag aaccgaacct tgcttgccgc
     1741 cctgcgcgcc gagctcaacc gacgcggagt gacgatcccc ctcgtgctgg gcaaccgcaa
     1801 ctcgccgccg ttcatcgatg aggcgttgcg cgagctcgac acggctggct gccggcgcgt
     1861 gctggcgttc gccaccagcc agtactcctc ctactcctcg tgccggcagt accgcgagaa
     1921 cctcgccgag gcactgagca gcacggggct cgcggaccgg atgcaggtgg ccaaggtgcg
     1981 gcccgcgttc gatcgggccg gattcgtcga gacgaccgtc gagctcctcg cctcagcggt
     2041 gtccggccgc gcacgtgagg gtcggcgtgt gctgttcacc acgcactcca tcccgtcggc
     2101 agacgcggcg gcctccggtc cgggcgcgga acccggcgag gtggacctct actctgcgca
     2161 gcaccgggag gtggcgaacg ccgtgatcgg tggggttgcg gcactcgcca gcgagaagcc
     2221 gttgccgtgg cgcctggtct accagtcccg ctccggctcg ccacgtgtgc cgtggctcga
     2281 gccggacatc aacgacgccc tg
//
LOCUS       DASRKH010000429         3116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_6872, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000429 DASRKH010000000
VERSION     DASRKH010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6872"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="VFC06_06880"
     CDS             <1..619
                     /locus_tag="VFC06_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HZL81639.1"
                     /translation="YPSQSGTEVPRDFVEYPSQVNEMWAWDPTILRSYAVHHVTGEPM
                     PPEWVDTLLAARQDGEGFATTEYLAAALLDQAWYRLAPDEVPADPADVEAFEAAALER
                     AGVAFAPVPPRYRTAYFNHIFASGYSAGYYSYIWSEVLDADTVEWFGEHGGLSRANGE
                     EFRRRLLARGGSTDPMAAFRDLRGRDPEIAPLLARRGLLAATTEA"
     gene            641..2005
                     /locus_tag="VFC06_06885"
     CDS             641..2005
                     /locus_tag="VFC06_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZL81640.1"
                     /translation="MSTGPARPSSAAPACLPTGSSAAEDEVVGLCQDLIRFDTSNYGD
                     GSGPGERAAAEHVMGLLTDVGLEPELFESEPGRANVVVRLEGADSARPALVLHGHLDV
                     VPAHAPDWQVDPFGGELRDGLVWGRGAVDMKDMDAMILAVVRQMVREGRRPSRDVVVA
                     FFADEEAGGAKGASWAVDHRPELFAGATEAISEVGGFSVDLDGHRAYLLQTAEKGIGW
                     LRLVADGRAGHGSQINTDNAVTHLAAAVGRIGQHPWPLSLSPTVRTLLQGVADLVGLS
                     FDPEDEHSIDAILAALGSASRFVGATTRNTANPSGLTSGYKANVIPGHAEATVDGRFL
                     PGQEEEFLATLARLAGPDVALEHIHRDVALEVPFSGGLVDAMVGALVVEDPGAVVLPY
                     MLSAGTDNKSLSRLGIAGYGFAPLRLPAELDFAGMFHGVDERVPVDALRFGVRVLDRL
                     LRSC"
     gene            complement(2008..2259)
                     /locus_tag="VFC06_06890"
     CDS             complement(2008..2259)
                     /locus_tag="VFC06_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5703 family protein"
                     /protein_id="HZL81641.1"
                     /translation="MTETAAVRSLRGDLAAQSAHYEFRVLSIPRSTSPGDASRRLTEE
                     AEYGRWELARVVLYVGGERKVTLRRKIIRVHSTLDYTIG"
     gene            2355..2678
                     /locus_tag="VFC06_06895"
     CDS             2355..2678
                     /locus_tag="VFC06_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein"
                     /protein_id="HZL81642.1"
                     /translation="MTVDPRAALDRLIAALEAHYNAVASRRGEDDPGVDDAYDVLADA
                     FEAYDDALGTVYSEATPFYLAEEDDEDDEDDESDGDDAEESDDADDEYDDDVELVDEL
                     TTEDR"
     gene            complement(2738..>3116)
                     /locus_tag="VFC06_06900"
     CDS             complement(2738..>3116)
                     /locus_tag="VFC06_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZL81643.1"
                     /translation="LGIGILAWSPLGRGVLTGKYQRSIPADSRAASPHLAGFVEPYLG
                     SQSLAVVEAVATAAAGLDCTPLEVALAWLRMRTQVTSSIVGARTAAQLRGSLAADDVE
                     LPAEIVRALDEVSAPELGYPERR"
BASE COUNT          428 a         1120 c         1128 g          440 t
ORIGIN      
        1 ctacccctcg cagtcaggca ccgaggtgcc gcgcgacttc gtcgagtacc cctcgcaggt
       61 caacgagatg tgggcgtggg acccgacgat cctgcgttcg tacgccgtcc accacgtgac
      121 gggcgagccg atgccacccg agtgggtcga cacgctgctc gcggcccgcc aggacggcga
      181 aggcttcgcg acgaccgagt acctcgccgc cgcgctcctc gaccaggcgt ggtaccggct
      241 cgcgcccgac gaggtcccgg ccgacccggc cgacgtcgag gcgttcgagg ccgccgcgct
      301 cgagcgggcc ggggtggcgt tcgcgccggt gccgccgcgg taccggacgg cgtacttcaa
      361 ccacatcttc gcgagcgggt actcggccgg gtactactcg tacatctggt ccgaggtgct
      421 cgatgcggac accgtcgagt ggttcggtga gcacggcggg ctgtcgcggg cgaacggcga
      481 ggagttccgg cgtcggttgc tggcgcgcgg ggggtcgacc gacccgatgg ccgcgttccg
      541 cgacctgcgg ggacgcgacc ccgagatcgc cccgctgctg gctcgccgag gcctgctggc
      601 ggccacgacc gaggcctgag cggcgggtac ggtgaacgcc atgtccaccg gccccgcccg
      661 cccgtcgtcc gccgcccctg catgcctccc cacagggtcc tcagccgctg aggacgaggt
      721 cgtcggactc tgccaggacc tcatccggtt cgacacctcg aactacggtg acggttccgg
      781 gccgggggag cgcgcggcgg ccgagcacgt catgggactc ctgaccgacg tcggcctgga
      841 gcccgagctc ttcgagagcg agccgggccg cgccaacgtc gtggtgcggc tcgagggcgc
      901 ggactcggcc cggcccgcgc tcgtcctgca cggtcacctc gacgtcgtgc cggcccacgc
      961 gccggactgg caggtcgacc cgttcggcgg cgagctgcgc gacggcctgg tgtggggccg
     1021 cggtgcggtc gacatgaagg acatggacgc gatgatcctg gcggtcgtgc gccagatggt
     1081 ccgcgaggga cgccgaccgt cccgcgacgt ggtcgtcgcg ttcttcgccg acgaggaggc
     1141 cggtggggcc aagggggcca gctgggcggt cgatcaccgg cccgagctgt tcgccggcgc
     1201 gacggaggcg atcagcgagg tcggcgggtt ctcggtcgac ctcgacggcc accgggccta
     1261 cctgctgcag accgccgaga agggcatcgg ctggctccgg ctggtcgcgg acggccgggc
     1321 ggggcacggc agccagatca acacggacaa cgccgtcacg cacctcgcgg ccgcggtcgg
     1381 gcgcatcggc cagcacccgt ggccgctctc gctcagcccg accgtgcgca cgttgctgca
     1441 gggcgtggcg gacctcgtgg gcctcagctt cgaccccgag gacgagcact cgatcgacgc
     1501 gatcctggct gcactcgggt cggcgtcgcg gttcgtgggc gcgacgaccc gcaacaccgc
     1561 gaaccccagc ggcctcacgt ccgggtacaa ggccaacgtc atcccgggcc atgccgaggc
     1621 gacggtcgac ggccggttcc ttcccgggca ggaggaggag ttcctcgcga ccctcgcgcg
     1681 gctcgccggt ccggacgtgg ccctcgagca catccaccgc gacgtggccc tcgaggtccc
     1741 gttctcgggt ggactcgtgg acgccatggt cggtgccctg gtcgtggagg acccgggtgc
     1801 ggtcgtgctg ccgtacatgc tctccgccgg gacggacaac aagtcgctgt cacggctcgg
     1861 tatcgccggc tacggcttcg cgccgctgcg gctgcccgcc gagctcgact tcgccgggat
     1921 gttccacggc gtcgacgaac gcgtcccggt cgacgcgctg cgcttcgggg tgcgcgtcct
     1981 ggaccgactc ctgcggagct gctgaggtca gccgatcgtg tagtcgagcg tgctgtgcac
     2041 gcggatgatc ttgcggcgca gcgtcacctt gcgctcgcct ccgacgtaga gcacgacgcg
     2101 ggcgagctcc cagcggccgt actcggcctc ctcggtcagc cgtcggctcg cgtcgcccgg
     2161 gctcgtcgac ctcgggatgc tcaggacccg gaactcgtag tgcgcactct gcgccgccag
     2221 gtcgccgcgc aggctgcgca ctgctgctgt ctctgtcact cgtcaccgtc cgatctcgct
     2281 acagaccggg cgacgcaggc gccgccgggc cgattgcagt gccattgtgc acggtgcgga
     2341 gataccgtca tgccatgacc gtcgacccgc gtgccgccct ggaccgcctg attgctgccc
     2401 tcgaggccca ctacaacgcc gtcgcatccc gtcggggcga ggacgacccg ggggttgacg
     2461 acgcctacga cgtgctcgcg gacgccttcg aggcctatga cgacgccctc gggaccgtct
     2521 acagcgaggc caccccgttc tacctcgcgg aggaggacga cgaggacgac gaggacgacg
     2581 agtccgacgg cgatgacgcg gaggagtccg acgacgcgga cgacgagtac gacgacgacg
     2641 tcgagctcgt cgacgagctg accaccgagg accgctgagg gctgcacagc accgttcggg
     2701 cacccggcat cattcgggtg ctgcaccgtt ctggtactca gcgccgttcg gggtagccga
     2761 gctcaggcgc gctgacctcg tcgagcgccc gcacgatctc cgcgggcagc tcgacgtcgt
     2821 cggcggcgag cgacccgcgc agctgcgcgg ccgtgcgcgc cccaacgatc gaggacgtga
     2881 cctgcgtgcg catgcgcagc cacgcgagcg cgacctcgag cggtgtgcag tcgaggcccg
     2941 cagcggcggt cgcgacggcc tcgacgacgg cgagcgactg cgagcccagg tacggctcga
     3001 cgaacccggc gaggtgcggc gaggcggccc tcgagtcggc cgggatcgag cgctggtact
     3061 ttccggtcag cacaccccgt ccgagcggtg accacgcgag gatcccgatg ccgaga
//
LOCUS       DASRKH010000430         2256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_54241, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000430 DASRKH010000000
VERSION     DASRKH010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54241"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..402
                     /gene="nadA"
                     /locus_tag="VFC06_06905"
                     /pseudo
     CDS             125..402
                     /gene="nadA"
                     /locus_tag="VFC06_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136936.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
     gene            507..1208
                     /locus_tag="VFC06_06910"
     CDS             507..1208
                     /locus_tag="VFC06_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81644.1"
                     /translation="MVAAVSGWPADDPLDPRAAALVVGEAWLAAVPGSEIEALPVGDG
                     GPRTADAWPGERSLVGGAEVAEHRGAQWLAPGGGAARWNPMDLSAALLGIAAAGASGP
                     VVIPVGDDTPAGDPADLWGGSLPALRHALRSLDLVVIATSTRPLLGFHGMSASLIDGR
                     EGDAALATAAQEQERRWTEIAREGDAVAGRESLVGPSRLSEHASCRPSRTWPRLRAWP
                     QPRWGRTWFLAWAAT"
     gene            1118..1420
                     /locus_tag="VFC06_06915"
     CDS             1118..1420
                     /locus_tag="VFC06_06915"
                     /inference="COORDINATES: protein motif:HMM:NF014634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HZL81645.1"
                     /translation="MPAFAYLAEASGLAAAAVGADLVLGVGGDLNPSTLDTGVAASVA
                     SAAARRGVPATMLSTAVKVGRRDLMAAGLASAHEAARGPSGLADGVRRVAQTWTPR"
     gene            1515..1868
                     /gene="erpA"
                     /locus_tag="VFC06_06920"
     CDS             1515..1868
                     /gene="erpA"
                     /locus_tag="VFC06_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HZL81646.1"
                     /translation="MTDTVTETSAHGVVITDAAASKVKSLLAQEGRDDLCLRLAVQPG
                     GCSGLIYQLYFDERTLDGDAVKDYDGVQVVVDKMSVPYLDGAKIDFADTIEKQGFTID
                     NPNAQSSCACGDSFS"
     gene            complement(1874..2122)
                     /locus_tag="VFC06_06925"
     CDS             complement(1874..2122)
                     /locus_tag="VFC06_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HZL81647.1"
                     /translation="MTEQPETAPIVVDARGKTCPMPIIMLAKAAAGLSPGTELTVLAT
                     DVAARYDVPAWARMTHHDFVGESETDGGALALTVRLRS"
BASE COUNT          349 a          733 c          790 g          384 t
ORIGIN      
        1 gtccagagca tcgtccgctg gccacccgtt gaccgccgcc actactcgca tggcaccaag
       61 tctgcacgct cacggcgtag agaagcgacg acctgcgctc ccgtccgcgt gaaacactgg
      121 ggacatgacg cagacgttcc aggaacccga ggcatctcct gcacttctcc tgttggggca
      181 acgcaccgac ccgatgtcgg agcgcggagt cgattgtccc ggcgacctcc cggagccttc
      241 cgaccccgat ctggtggagc gcgcacgcaa ggccaaggag acgctcggca gccgggtgtt
      301 cattctgggc caccactacc agcgcgacga ggtcatccag tttgccgacg tgacgggcga
      361 ctcattcaag ctggcccaac agaagaagcg ctggagaagc ctcgggttcc tggaacgtct
      421 gcgtcatgtc cccagtgttt catgcgcacg ggggcgccgg tcgtcgcttc actacgctgt
      481 gagcgtgcag acttggttcc atgcgagtgg tggcggcggt cagcgggtgg ccagcagacg
      541 atcctcttga cccgcgcgcg gcggcgttgg tagtggggga ggcgtggctt gccgcggtgc
      601 caggatccga gatcgaggcg ttgcccgtgg gcgacggcgg cccccgcacg gccgatgcat
      661 ggcccggtga gcgctcgctc gtgggtggcg ccgaagtggc cgagcatcgt ggcgcgcagt
      721 ggctcgctcc cggaggcggc gcggcccgct ggaaccccat ggatctctca gcggcgctgc
      781 tcgggatcgc tgccgcggga gcctccgggc cggtggtcat tcccgtgggc gacgacacgc
      841 cggcaggcga ccctgcagac ctgtggggcg gctcccttcc agccctgagg catgcactca
      901 gaagccttga cctcgtggtg attgccacct cgacaaggcc actcctgggc ttccacggca
      961 tgagtgcctc gttgattgat ggccgcgagg gcgacgccgc gctggccacc gccgcgcaag
     1021 agcaggaacg acgctggacc gagatcgctc gcgagggaga cgccgtcgcc gggcgagagt
     1081 cactcgtcgg cccgtctcgg ctctcggagc acgcatcgtg ccggccttcg cgtacctggc
     1141 cgaggcttcg ggcctggccg cagccgcggt gggggcggac ttggttcttg gcgtgggcgg
     1201 cgacctgaac ccctcgacac tcgataccgg cgtggccgca agcgtggcct cagcggctgc
     1261 gcgccggggc gtgccggcga cgatgctcag cacggcagtc aaggtcgggc gccgcgacct
     1321 catggctgcg gggctggcct cggcacatga ggcggcgcgg ggcccctccg gtctggcgga
     1381 cggtgtgcgc cgcgtggcgc agacctggac gcctcgttga acgggcacaa atcccaccgc
     1441 tttggtgttg ttgttcccag ggaggcgcac cgcgtcgtac ccttgagaga actcgtaccg
     1501 gaaggagtcg ccatatgacg gataccgtaa ctgagacttc ggcgcacggc gtggtcatta
     1561 ccgacgcggc tgcatcgaag gtcaagagcc tccttgcgca ggagggtcgc gacgatcttt
     1621 gtttgcgtct ggccgttcag cctggcggct gctcgggcct gatctaccag ctctacttcg
     1681 acgagcgcac gctcgacggc gacgccgtga aggactatga cggcgtccag gtggtcgtgg
     1741 acaagatgtc ggttccgtac cttgacggtg cgaagatcga cttcgcggac acgatcgaga
     1801 agcagggctt cacgatcgac aatcccaacg cgcagagttc gtgtgcgtgt ggtgactcgt
     1861 ttagctgatc atttcagctt cgcaaacgga cggtgagggc tagcgccccg ccgtccgttt
     1921 cgctttcccc cacaaaatcg tggtgagtca tgcgtgccca tgcgggaacg tcgtagcgcg
     1981 cggcaacgtc cgtcgcgagc accgtcaact cagtgccggg ggagaggccc gcggcggcct
     2041 tggccaacat gatgatgggc atcgggcagg tcttcccgcg tgcgtccacc acgatcggtg
     2101 cggtctccgg ttgctccgtc acgatgccgc attcacgatg cgatgtgcgg ggcgcccatg
     2161 cgcgagcgca cggcgtcgag cgcgtcgggg agctcgacga ggaagcgatc cacgtcctgc
     2221 tcggtgatcc ccgggtggag cgccacgcgc acgttg
//
LOCUS       DASRKH010000431         2715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_52170, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000431 DASRKH010000000
VERSION     DASRKH010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52170"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            382..585
                     /locus_tag="VFC06_06930"
     CDS             382..585
                     /locus_tag="VFC06_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81648.1"
                     /translation="MRQPDGYFGVPPEWRNRNVSGVLVVNQLMPYYVQRAETTLWRQS
                     APSISYMTRLDSQGMYLTSARVS"
     gene            complement(713..1732)
                     /locus_tag="VFC06_06935"
     CDS             complement(713..1732)
                     /locus_tag="VFC06_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZL81649.1"
                     /translation="MRIGLIASPWVAVPPPAYGGTEMVVDILARGLQDEGHEVLLAAA
                     ADSTCPVPLVPGTAMADPDAINQTGSVLAHVIRAYGAMASMDLVHDHTLLGPLYRHRP
                     AGVPVVVTNHGPFTAETRTIFRAMAPEVAIVAISHDQASRAEDIPIARVIHHGMDVSA
                     VPVGSGDGGYACFLGRMCADKGVAEAIAVARAAGVPLRIAAKMRESAERQFFRECVEP
                     LLGPDAEYVGELAGAEKYELLGGAFALLNPIQWPEPFGLVMIEALATGTPVVGTPHGA
                     APEIVRDGVTGFLAPVEELADLLRRATELDRAACRADVETRFSADRMVADHLRLYRDI
                     LGVSG"
     gene            1991..>2715
                     /locus_tag="VFC06_06940"
     CDS             1991..>2715
                     /locus_tag="VFC06_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008736107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAzo13 family transposase"
                     /protein_id="HZL81650.1"
                     /translation="MAVSGDVVGVLTAEFEALLPHLDERSVRLVLAARARSLGHGGIV
                     AVARASGSSRSRVQQGVAELESGAGPLGRVRRPGGGRKPATQADPGLLPALLALVEPT
                     RRGDPESALSWTTLSVRRLAAELTAAGHAVSQETVAKLLRAEGFSLQANAKTVEGGGH
                     PDRDAQFSYLNSRAEQHLEAGQPVISVDTKKKELVGNYQNGGAEWRPAGDPEQVKVHD
                     FIDPQLGKANPYGIYDIAGNTGWV"
BASE COUNT          424 a          929 c          939 g          423 t
ORIGIN      
        1 atcaactacc gcgagcagcg catccgtctc gacgtgacgc cgtcgaccct ccgcatggag
       61 gtcgggccgg gcggtcgtgt cccgatccgg gtcgtggtgg agggggagga gaacgtgctg
      121 gcacccggcg aaacccgcga cttcgcgatc cggtccgact gaaccgtgat ccaccgacgt
      181 tgatttcgtg agcgttttcg ttaacggcct ggagcgctgg ggtgcgtggg ggcgtggagg
      241 gtccgccggc ctgtaccggc ttttccacat cgggggttcc tcgggttgcg ccctcgcggg
      301 cgaggttgaa cgcgatgacc gcgcaggcga cccaggcggc gttcgctgtc gtgccggacg
      361 aggctcgacg ccagttgggc ggtgcggcag ccagatgggt acttcggcgt tccacccgag
      421 tggcgaaacc gcaacgtgtc gggcgtcctc gtggtcaacc agctgatgcc ctactacgtt
      481 cagcgcgcgg agacgaccct gtggcggcaa tcggccccct ccatcagcta catgacgaga
      541 ctggattccc agggcatgta cttgacctcc gcgagggtgt cctgatcgaa accacggcgg
      601 tcgggtagcg gcgcccgtcg gcgtcgtccc ccgggggcag gtggtatgcg tactcgatca
      661 cgccgcccag gacatgtgag aacgtccggg cgacccccag cgaccccacc tgctacccgc
      721 tcacgccgag gatgtcgcgg tacaggcgca ggtggtccgc gaccatgcgg tccgcactga
      781 atcgcgtctc gacgtccgcc cggcatgccg cccgatccag ttcggtggcc cgccgcagca
      841 ggtccgcaag ctcctccacc ggagcgagga agccggtgac gccgtcgcgg acgatctcgg
      901 gagccgcccc gtggggcgtg ccgaccaccg gtgttccggt ggcgagcgcc tcgatcatca
      961 ccagcccgaa gggctcgggc cactggatgg ggttgaggag cgcgaacgcg ccgcccagca
     1021 gctcgtactt ctccgcgccc gccagctccc cgacatactc cgcatccggc cccagcaacg
     1081 gctcgacaca ctcgcggaag aactgccgct cggcggactc acgcatcttc gcggcgatcc
     1141 tcagcggtac acccgccgcg cgcgcgaccg cgatcgcctc cgccacgccc ttgtcggcgc
     1201 acatgcggcc gaggaagcag gcatatcccc catcgccgga gcccaccggc accgccgaca
     1261 cgtccatgcc atggtggatg acacgggcga tcgggatgtc ctccgccctg ctcgcctgat
     1321 cgtgggagat ggccactatg gccacctccg gtgccatcgc acggaagatc gtgcgcgtct
     1381 cagcggtgaa cggcccgtgg ttggtcacca ccaccgggac tcccgccggc cggtgccggt
     1441 acaacggtcc gagcagggtg tgatcgtgca ccagatccat ggacgccatc gcgccgtacg
     1501 cgcggatcac gtgcgccagg accgacccgg tctggttgat cgcatccggg tccgccatgg
     1561 cggtacccgg caccagcggc acgggacagg tgctgtccgc cgcggccgcg agcagcacct
     1621 cgtggccttc gtcctgcagc ccacgggcca ggatgtccac caccatctcg gtgcccccgt
     1681 aggcgggtgg cgggaccgcc acccagggtg atgcgatcag gccgattctc atgggtcctc
     1741 tcctcctcgt cgtctcctcg ccacgtcgcg gggcatcggc tcccggtgga gcgtcagcct
     1801 ctccggcaga ccgatcgggc gccttgttgt cgccgacgat gccactccac accgccgcag
     1861 caacacctgc aacaattctc ccggacccac cctcagaacc cggacaccgc cacacgaaca
     1921 aacaccaccc acgttaggcc acgtcaataa ataagtaggt ctgatcgtgt tgtgtggctg
     1981 ataatggggt atggctgttt ctggggatgt ggtcggggtg ctgacggcgg agttcgaggc
     2041 attgttgccg catctggatg agcgttcggt gcggttggtg ctcgccgcgc gggcgcggtc
     2101 gttgggtcac ggtgggatcg tggcggtggc gcgggcgtcg gggtcgtccc ggtcccgggt
     2161 ccagcagggg gtggcggagc tggagtcggg ggccggtccg ttgggccggg tgcgccggcc
     2221 cggcggtggg cgcaaaccgg cgacccaggc cgatccgggt ctgctgccgg cgttgctggc
     2281 gctggtggaa ccgacccgcc gcggggatcc ggaatccgct ctgtcttgga cgacgctgtc
     2341 agtacgcagg ctcgccgcgg agctgacggc cgccgggcat gcggtgagcc aggagacggt
     2401 ggccaaactg ctgcgtgccg agggcttcag cctgcaggcc aacgccaaga cggtcgaggg
     2461 cggagggcac ccggaccggg atgctcagtt ctcctacctc aactcccggg ccgagcagca
     2521 tctcgaagcc gggcagccgg tgatcagtgt ggacaccaag aagaaggaac ttgtcgggaa
     2581 ctaccagaac ggcggcgccg agtggcggcc ggccggcgac cccgaacagg tcaaggtgca
     2641 cgacttcatc gacccgcagc tcggcaaggc gaacccatac gggatctacg acatcgccgg
     2701 gaacaccggc tgggt
//
LOCUS       DASRKH010000432         2614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_23499, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000432 DASRKH010000000
VERSION     DASRKH010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23499"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /locus_tag="VFC06_06945"
     CDS             <1..1072
                     /locus_tag="VFC06_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012877949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZL81651.1"
                     /translation="GAHSLAEVAHAYGADIDVPDVVPVPSDHLDVARAAPDADLADVL
                     GQDEARTALEVAAAGGHHLLMVGPPGSGKTMLAARLPGLLPDLTEAEAVEVTAVHSVA
                     GTFDPGGGLLRRPPFEDPHHTATPASIVGGGSGVPRPGAASRAHRGVLFMDEAPEFST
                     AVLQTLRQPLEHGELVIHRASGVARFPARFQLVLAANPCPCGRGVGKGLECTCRSEQR
                     RRYFSKLSGPLLDRVDLQVDVLPVSRAALDPGTVAESSCVVAGRVAAARAAQAGRLAG
                     TPWRTNAEVPGVWLRERLGPDRRLVSDLDRALDRGLLSLRGVDRVLRVAWTLADLAGR
                     SGPRRDDIGQALLLRTRGQGLA"
     gene            1069..2286
                     /gene="dprA"
                     /locus_tag="VFC06_06950"
     CDS             1069..2286
                     /gene="dprA"
                     /locus_tag="VFC06_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HZL81652.1"
                     /translation="MSRLRLDVNDELSARMAWSRLTEPGDAVAGELVRTLGAGRALAW
                     AHSAAACGDGTPRAIAALVDAEPGLEDVRRQLGKAVARWAGRFADLDPARDLEHMDHL
                     GGSVLHPGSVGWPTGLDDLGASAPMCLWVRGRRDVSASVDRSIALVGARASTSYGEHV
                     AVDLAGGLAAHGACVVSGGAYGIDAAAHRGSLAAGGTTVALLAGGVDRAYPVGNAPLL
                     ERILEQGGSVLSEVPPGGVPSKGRFLMRNRLIAAVSRATVVVEAAWRSGALSTANHAV
                     ALLRPVGAVPGPVTSMASAGCHRLLREGVAVCVTDAAEVLELAGRAGEDLPPDRVVEP
                     RDGDDLDWVSRRLLDALPGRSSAGHDELARMAGLSVGEVRGALGVLELAGFVRRVGAG
                     WARSGRAGAGRRG"
     gene            2508..>2614
                     /locus_tag="VFC06_06955"
     CDS             2508..>2614
                     /locus_tag="VFC06_06955"
                     /inference="COORDINATES: protein motif:HMM:NF014898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HZL81653.1"
                     /translation="MHLRAQRGLSEHTVRAYVSDVEHLMNFAGRYGRTE"
BASE COUNT          299 a          922 c         1017 g          376 t
ORIGIN      
        1 cggtgcgcac agccttgcgg aggtcgcgca cgcctacggc gccgacatcg acgtccccga
       61 cgtcgtcccc gtgccctccg accacctcga cgtcgcccgt gcggcgccgg atgccgacct
      121 cgccgacgtg ctcgggcagg acgaggcgcg cacggcgctc gaggtggcgg ccgcgggcgg
      181 ccaccacctg ctcatggtcg gcccgccagg ctcgggcaag accatgctcg cggcgcgcct
      241 gccgggtctc ctgcccgacc tgaccgaggc cgaggcggtc gaggtcacgg cggtccactc
      301 ggtcgcgggg acgttcgacc ccggcggtgg tctgctgcgc cggccgccgt tcgaggaccc
      361 gcaccacacc gcgacacccg cgagcatcgt gggtgggggc tcgggtgtcc cacggcccgg
      421 cgcagcatcc cgggcgcacc gcggcgtcct gttcatggac gaggcgccgg agttctccac
      481 cgctgtgctg cagacgttgc gccagccgct cgagcacggc gagctcgtca tccaccgcgc
      541 ctcgggcgtt gcgcgcttcc ctgcgcgctt ccagctcgtg ctcgcggcca acccgtgccc
      601 gtgcggccgc ggcgtcggca aggggctcga gtgcacctgc cggtcggagc agcgccgccg
      661 gtacttcagc aagctgtcag gtccgttgct ggaccgtgtc gaccttcagg tcgacgtcct
      721 gccggtgtcc cgggctgcac tcgacccagg gaccgtcgcg gagtcgagct gcgtcgtcgc
      781 gggacgcgtc gcggcggcac gcgccgcgca ggcaggacgg ctcgccggta caccctggcg
      841 gaccaatgcc gaggtgccgg gcgtgtggct gcgtgagcgg ctggggccgg accgtcggct
      901 cgtgagcgac ctcgaccgcg cgctcgaccg cgggctgctc agtctgcgcg gcgtggaccg
      961 cgtgctccgg gtggcctgga cgctcgccga cctcgcgggg cggtcgggcc cacgccgcga
     1021 cgacatcggt caggcgctcc tgctgcgcac gcgcggacag gggctcgcgt gagcaggctg
     1081 cggcttgacg tcaacgacga gctctcggcc cgcatggcct ggagccggtt gaccgagccg
     1141 ggagacgcag tcgcgggcga gctcgtccgg acgctgggtg ccggccgggc gctggcctgg
     1201 gcgcacagcg cagccgcgtg cggtgacggc accccccgtg cgatcgccgc gctcgtcgat
     1261 gccgagcccg ggctcgagga cgtgcgacgc cagctcggca aggcagtcgc gcgctgggcg
     1321 gggcggttcg ccgacctgga cccggcccgc gacctcgagc acatggacca cctcggcggc
     1381 agcgtgctgc accccggatc ggtcggctgg ccgaccggtc tcgacgacct cggagcgagc
     1441 gcgccgatgt gcctgtgggt tcgcgggcgg cgcgacgtgt ccgcatccgt ggatcgctcg
     1501 atcgcgctcg tgggggctcg cgcttcgacg tcctacggtg agcacgtcgc ggtcgacctc
     1561 gccggcggtc tggccgcaca cggtgcgtgc gtcgtctcag ggggcgccta cgggatcgac
     1621 gcggccgcgc accgcggttc cctggctgcc ggcggcacga ccgtggcgct gctcgcgggc
     1681 ggcgtcgacc gcgcgtaccc ggtcggcaac gcgccgctcc tcgagcgcat cctcgagcag
     1741 ggcggcagcg tgctgagcga ggtgcctccg gggggcgtgc cgagcaaggg caggttcctc
     1801 atgcgcaacc ggctgatcgc ggccgtgagc agagccacgg tggtcgtcga ggcggcatgg
     1861 cggtcgggcg cgttgagcac ggcgaaccat gcggtcgccc tcctgcgccc ggtcggtgcg
     1921 gtgccgggtc ccgtgacctc gatggcgtcg gccgggtgcc accggttgct ccgggagggg
     1981 gtggccgtct gcgtgaccga cgctgccgag gtgctggagc ttgcggggcg cgcgggcgag
     2041 gaccttcccc ccgaccgtgt ggtcgagccc cgcgacggcg acgacctcga ctgggtgtcg
     2101 cgccgattgc tcgatgctct gccgggccgc tcgtccgcgg ggcacgacga gctcgcacgc
     2161 atggccgggc tgtccgtggg cgaggtgcgc ggcgcgctgg gcgtgctcga gctcgccggg
     2221 ttcgttcgcc gtgtcggtgc cggatgggcc cgttcgggtc gcgccggcgc ggggcgccga
     2281 ggatgagggg gtacgaacag agagggtgcg gagtctccct gaacggatcg aaccgtgcaa
     2341 cgttgtggcc gaattcggac aaggcgccgc gtatcaccca ggcacgggtg aagtctgccg
     2401 ggcgtcgctt gtgagtcatg tgtccgacac ggcacggtag gggcatgcac tcggccacgg
     2461 gcacgagcgc ggcaacggtc tggggggccg ctgtcgcgga cttcgccatg catctgcggg
     2521 ctcagcgggg cctctcggag cacaccgtgc gtgcgtacgt gagcgatgtc gagcacctga
     2581 tgaacttcgc tggtcgatac ggtcggactg agat
//
LOCUS       DASRKH010000433         2244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_33977, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000433 DASRKH010000000
VERSION     DASRKH010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33977"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1325
                     /locus_tag="VFC06_06960"
     CDS             <1..1325
                     /locus_tag="VFC06_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZL81654.1"
                     /translation="QRSGDDRPRRPYEGGGDRPQRSYGDRPQRSGDDRPRRPYEGGGD
                     RPQRSYGDRPPRTSDRDRLRQDDRGDRRYDDRGPSVGRPQWEDRHDDSHDRRRVERAA
                     ERAAEPPLDDDITPQMLDKESRTHLRTLNKENSDYVARHLVAAGQYLDTDPELAYQHA
                     LAAQRRAGRVDVVREAVALTAYQTGRYADALREARTVRRLSGDDSLRAIEADSERGLG
                     RPERALAVIAEVELAHQPAHARTELAIVASGARADLGEFEAGLLVIEDALRFIRDVDL
                     RGRLLSVKADRLDDLKRTEEANEVRAMLEAEPSEDDESVLYIDETLDEELAMAVDLED
                     AADREREVASDEDADEESDFDEESGEPFNVVGDVDDVREPYGHDSEDDDFDDDADSDE
                     ASGEPLKVVGDLDDVREPYGPAEPDGDDVAGGESPEDEMAGDGERDER"
     gene            1322..>2244
                     /locus_tag="VFC06_06965"
     CDS             1322..>2244
                     /locus_tag="VFC06_06965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HZL81655.1"
                     /translation="MTTLLASEVPLAEVHDVALMDLDGVTYEGAAPIEYAAEGLASAR
                     GRGLRLFYVTNNSSRRPEDVAGQLTSLGMPATPDEIFSSAMAAVQLAVGRHGLGARVL
                     IVGGPGLVQAAQEGGLTLVESADDKPVAVIQGFRESICWRDLSEVAYAINGGAEFIAT
                     NLDSTLPRERGLAVGNGSLVAAVANATGVTPLSAGKPEPEVFRLAAERAGATNPLAVG
                     DRLNTDIAGAVASGVPSLHVLTGVSKARDVVTAPASQRPNYLGIDLRDLTRPHPVVEY
                     DGEWAVCRAARARVADGRLELDRDGIREFADA"
BASE COUNT          354 a          767 c          823 g          300 t
ORIGIN      
        1 cgcagcgcag tggtgatgac cgcccgcgtc gcccctatga gggtggcggg gaccgccccc
       61 agcgttccta cggtgaccgc ccgcagcgca gtggtgacga ccgcccgcgt cgcccctacg
      121 agggtggcgg ggaccgcccc cagcgctcgt acggtgaccg gcctccccgc acctctgacc
      181 gggaccgcct gcgccaggat gacaggggag atcggcgtta cgacgaccgc ggcccgtcgg
      241 ttggccgacc gcagtgggag gatcgtcacg acgactccca cgatcgccgt cgggtggagc
      301 gcgccgccga gcgcgccgcg gagccgcccc tggatgacga catcacgccg cagatgctcg
      361 acaaggagtc gcgcacccat ctgaggaccc tgaacaagga gaactcggac tacgtggcgc
      421 gccacctggt ggccgccggc cagtacctcg acacggaccc tgaactggcg taccagcacg
      481 ccctcgcggc ccagcggcgt gccggtcgcg tggacgtggt tcgcgaggcg gtggcgctga
      541 ccgcctacca gaccgggcgt tatgcggatg ctctccgtga ggcccgcacc gtgcgacggc
      601 tctccggcga cgactcgctg cgggccatcg aggctgattc cgaacgcggg ctggggcgtc
      661 cggagcgagc actcgccgtg atcgccgagg tcgaactggc ccaccaaccg gcccacgcgc
      721 gcacggaact cgccatcgtg gcgtccgggg cgcgggcgga cctcggtgag ttcgaggccg
      781 gcctcctcgt catcgaggat gcgctcaggt tcattcgcga cgtcgatctg cggggacgcc
      841 tcctgagcgt gaaggccgac cgcctcgacg acctcaaacg caccgaggag gccaacgagg
      901 tcagggcgat gctggaggcc gaaccgtccg aggacgacga gtcggtcctc tacatcgacg
      961 agaccctcga cgaggaactc gcgatggcgg tggacctcga ggacgccgcg gatcgcgaac
     1021 gggaggtggc ctctgacgag gatgcggacg aggaatccga tttcgacgag gaatcgggcg
     1081 aaccgttcaa cgtggtgggt gacgtcgacg acgtccgtga gccctacggg cacgactccg
     1141 aggacgacga cttcgacgac gacgctgact cggacgaggc gtcgggcgaa ccgctcaagg
     1201 tggtgggtga cctcgacgac gtccgcgagc cgtacggtcc cgccgaaccc gatggtgacg
     1261 atgtcgcagg cggcgagtcg cccgaggacg aaatggcggg cgacggcgaa cgggacgagc
     1321 gatgacaacc ctgctggcga gtgaggttcc gcttgcggag gtgcacgacg tcgcgctgat
     1381 ggacctcgac ggagtcacct acgagggcgc cgccccgatc gagtacgcgg ccgaggggtt
     1441 ggcgtccgcg agggggcgcg ggctccggct gttctatgtc acgaacaact cgtcgcgcag
     1501 accggaggac gtcgcgggcc aactcacgag cctcggcatg ccggcaacac ctgacgagat
     1561 cttctcctcg gcgatggcgg cggtgcaact tgcggtcggg cggcacggcc tcggggcgag
     1621 agtcctcatc gtgggcggtc ccgggctcgt ccaggccgcc caggagggtg ggctgaccct
     1681 ggtggagagc gcggacgaca agccggtcgc cgtgatacag ggcttccggg agtcgatctg
     1741 ctggcgggac ctgtcggagg tcgcctacgc gataaacggc ggcgccgagt tcatcgccac
     1801 caacctcgac tccacgctgc cgcgggaacg gggcctggcg gtgggcaacg ggtcgctggt
     1861 ggccgccgtc gcgaatgcga cgggtgtgac gccattgtcg gcgggcaagc cggagcccga
     1921 ggtcttccgc ctcgcggcgg agcgcgcggg tgcgacgaac cccttggccg tcggcgaccg
     1981 cctcaacacg gacatcgccg gcgcggtggc ctccggcgtg ccgtccctgc acgtcctcac
     2041 cggcgtctcc aaggcgcggg acgtcgtcac ggctccggcc tcacaacgcc cgaactacct
     2101 cggcatcgac ctgcgtgacc tcacgcggcc gcacccggtc gtcgagtacg acggcgagtg
     2161 ggccgtgtgc cgcgccgccc gcgcccgtgt ggcggacggt cgcctggaac tggaccgcga
     2221 cggcatccgc gagttcgcgg atgc
//
LOCUS       DASRKH010000434         2756 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_42704, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000434 DASRKH010000000
VERSION     DASRKH010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42704"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..701)
                     /locus_tag="VFC06_06970"
     CDS             complement(135..701)
                     /locus_tag="VFC06_06970"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HZL81656.1"
                     /translation="MSMRIVVLGAPGAGKGTQAARISDHFGTPTISTGELFRSHIAAG
                     DELGQLAAGYIDQGNLVPDDVTTLMVADRLSRDDVAGGFILDGFPRTLAQAAALDRIL
                     GDRPLDGVVDFEVQIDAVIGRMLRRAVEQGRSDDTEDVIRNRIRVYHEKTAPLAAHYR
                     ERGLLISVDAMGEIDEVASRVVDALEHI"
     gene            complement(698..1987)
                     /gene="secY"
                     /locus_tag="VFC06_06975"
     CDS             complement(698..1987)
                     /gene="secY"
                     /locus_tag="VFC06_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HZL81657.1"
                     /translation="MLSAFAQAFRTPDLRRKLLFTLGIMALFRLGAFIPAPGVSYANV
                     QQCAAASEASIMDMVNLFSGGGLLQLSIFALGIMPYITASIIIQLLRVAIPKFEELHK
                     EGQAGTAKLTQYTRYLTIGLGVLQSVTVVTMAVSGRLYPGCPVSPIPDESPWNLFLVV
                     LAMTAGTGLIMWLGELITERGVGNGMSLLIFTSIVASFPTQMISLAGGANGFRNIVIM
                     LAVILLITLAVVFVEQSQRRIPVQYAKRMVGRRLYGGSTTYIPLKINQAGVIPVIFAS
                     SILQLPVLAAQFAPATSWSLWLQQHFLVRQSGWYMVTYALLIIFFAFFYTSITFNPDE
                     VADNMKRYGGFIPGIRAGRPTAEYLHYVMTRLTWPGSLYLAVVAMIPVLVIASMGVSQ
                     QMRFGGTTIMIMVGVGLQTVKEIDSQLQQRHYEGFLQ"
     gene            complement(2136..2600)
                     /gene="rplO"
                     /locus_tag="VFC06_06980"
     CDS             complement(2136..2600)
                     /gene="rplO"
                     /locus_tag="VFC06_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012877428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HZL81658.1"
                     /translation="MAEETEKINVLKIHHLRPAPGAKTVKRRVGRGEGSKGKTAGRGT
                     KGTKARYQVPARFEGGQMPLHMRLPKLRGFKNPFRVEYQVVNLDKLAELFPEGGRVEV
                     ADLVEKGAVRKGHLVKVLGTGDVAVRLDIEADAWSNAAKQKIEAAGGSIAQP"
     gene            complement(2600..>2756)
                     /gene="rpmD"
                     /locus_tag="VFC06_06985"
     CDS             complement(2600..>2756)
                     /gene="rpmD"
                     /locus_tag="VFC06_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HZL81659.1"
                     /translation="KSAIGGKQNQRETLRSLGLRKIGQTVERPDDGVTRGMIRAVAHL
                     VTVEEVK"
BASE COUNT          470 a          914 c          908 g          464 t
ORIGIN      
        1 gtccagttcc gccgtcgtga ccccgggcgc gatggccgca cgaacctccg cgagcatgcc
       61 ggccacgatg tgcccggcac tacgcatgag gcggatctcg tcgagcgtct tcagctggat
      121 ccgctcacgg aacactagat gtgctccagg gcgtcgacca cgcgggatgc cacctcatcg
      181 atctcaccca ttgcatcgac gctgatgagg agtccacgtt cgcggtagtg ggcggccagt
      241 ggggcggtct tctcgtggta gaccctgatg cggttgcgga tcacgtcctc cgtgtcgtcg
      301 ctgcggccct gctcgaccgc ccggcggagc atgcgcccga tcaccgcgtc gatctggacc
      361 tcgaagtcga cgacgccgtc aagtggacgg tcaccgagga tgcggtccaa cgcggccgcc
      421 tgggcgaggg tgcgggggaa cccgtcgagg atgaacccac cggcaacgtc gtcgcgggac
      481 aggcggtcgg cgaccatcag ggtggtgacg tcgtcgggga cgaggttccc ctggtcgatg
      541 tacccggcgg cgagctgtcc cagctcgtcg cccgcggcga tgtggctgcg gaacagctcc
      601 cccgtggaga tcgtcggtgt gccgaaatgg tcactgatgc gcgcggcctg ggtacccttg
      661 cctgcacccg gcgctccgag caccacgatg cgcatgctca ttgcaggaat ccctcatagt
      721 gccgctgctg cagctgggag tcgatctcct tcacggtctg caggccgacg cccaccatga
      781 tcatgatggt cgttccaccg aaccgcatct gctgggacac acccatggaa gcgatcacca
      841 gaacggggat catggccacc accgcgaggt agagggaacc cggccacgtc aggcgggtca
      901 tcacatagtg caggtactcc gcggtcggcc ggccggcgcg gataccgggg atgaagccgc
      961 cgtagcgctt catgttgtcc gccacctcgt cgggattgaa ggtgatggag gtgtagaaga
     1021 aggcgaagaa gatgatcagc agggcgtacg tcaccatgta ccacccgctc tgccgcacca
     1081 ggaagtgctg ctggagccac agcgaccagc tcgttgccgg ggcgaactgc gccgcgagca
     1141 ccgggagctg gaggatcgag gacgcgaaga tcaccgggat cacgccggcc tggttgatct
     1201 tcagcgggat gtaggtggtg gagccgccgt agaggcggcg ccccaccatg cgcttggcgt
     1261 actggaccgg gatgcgccgc tgcgactgct ccacgaacac caccgcgagg gtgatcagca
     1321 ggatcaccgc cagcatgatg acgatgttcc ggaacccgtt ggcgccaccg gcgagggaga
     1381 tcatctgagt ggggaaggaa gccacgatcg acgtgaagat caggagcgac atgccgttgc
     1441 cgacgccgcg ctccgtgatc agctcaccga gccacatgat gagccccgtg cccgccgtca
     1501 tcgccaggac caccaggaag aggttccacg gcgattcgtc cgggatcggg ctgacggggc
     1561 agccggggta cagccggccc gagaccgcca tggtcaccac agtcaccgac tggagcactc
     1621 cgagaccgat ggtgaggtac cgggtgtact gggtcagctt ggccgtaccg gcctgaccct
     1681 ccttgtgcag ctcctcgaac ttcgggatcg ccacccgcag cagctggatg atgatcgacg
     1741 cggtgatgta cggcatgatg ccgagcgcga agatcgacaa ctggagcagc ccaccacccg
     1801 agaacaggtt gaccatgtcc atgatcgacg cttccgacgc cgccgcgcac tgctgcacat
     1861 tggcgtagct gaccccgggt gcggggatga acgcccccag ccggaacagg gccatgatgc
     1921 cgagcgtgaa cagcaacttg cgtcgcaggt caggcgttct gaacgcctgt gcgaatgcgc
     1981 tgagcaaggt tcctccaaat gcgactccgg gccaccggtg gtcagcctat cgtgtttcac
     2041 ccacaccccc tgcggctcgt gccggctggg gggaatgctg tccggaacag agctgccccc
     2101 gtaccaggac gggggcagct ctgggatgct gtggcctacg gctgcgcgat actgccgccg
     2161 gcggcctcga tcttctgctt cgcggcattc gaccaggcgt cagcctcgat gtcgagccgc
     2221 acggccacgt cgcccgtgcc gagcaccttc accaggtggc ccttgcgcac ggcgcccttc
     2281 tcgacgaggt cggctacctc cacgcggcca ccctcgggga acagctcggc gagcttgtcg
     2341 aggttgacca cctggtactc gacgcggaac gggttcttga acccgcgcag cttcggcagc
     2401 ctcatgtgca gtggcatctg gccgccctcg aaccgagcgg gaacctggta acgggccttc
     2461 gtgccctttg tgccacgccc ggcggtcttg cccttcgagc cctcaccgcg tccgacacgc
     2521 cgcttgacgg tcttcgctcc cggcgcggga cgcaggtggt ggatcttgag aacgttgatc
     2581 ttctcggtct cctcagccat cacttcacct cctcgacggt gaccaggtgg gccaccgcgc
     2641 ggatcatgcc gcgcgtcacg ccgtcatcag gccgttcgac cgtctggccg atcttcctga
     2701 gtccgagcga gcgcagcgtt tcccgctggt tctgcttgcc gccgatggcg gacttg
//
LOCUS       DASRKH010000435         3041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_40008, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000435 DASRKH010000000
VERSION     DASRKH010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40008"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VFC06_06990"
     CDS             complement(<1..325)
                     /locus_tag="VFC06_06990"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase, GntK/IdnK-type"
                     /protein_id="HZL81660.1"
                     /translation="MRVIVMGVTGCGKSSVGAALAAEMGYEFADADDFHPPASVAAMA
                     KGIPLTDEERWPWLDAVGAWMKPQTDVVVACSALRRSYRNRIREAAGPTMFLHLTATQ
                     SVIEER"
     gene            509..2101
                     /locus_tag="VFC06_06995"
     CDS             509..2101
                     /locus_tag="VFC06_06995"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZL81661.1"
                     /translation="MTTETFADFGVNPLIVDALVDHGIIAPFPIQSMTLPVALSGHDI
                     IGQAKTGTGKTLGFGVPLLHRAVSPGEPGFDDMGDASGKPQALVVAPTRELAVQVAND
                     LEMASKKRSTRILKIYGGRAYEPQIDALRKGVEVVVGTPGRLIDLMNQGHLDLRYVRT
                     VVLDEADEMLDLGFLGDVEKLLASTPAGRHTMLFSATMPGPVVALARRYMTQPTHIRA
                     SEPDDDGATVKATAQFVYRAHALDKIEVLARILQAEGRGLTIVFARTKRTCAKVSDDL
                     RDRGFAAGALHGDLGQGAREQALRAFRAGKIDVLVATDVAARGIDVEDVTHVINYQCP
                     EDEKTYVHRIGRTGRAGKKGVAVTFVDWDDMPRWGMIDRSLDLGYPEPEETYSNSPHL
                     YAELSILTGKKGTLPRSSRVREGLDAEKVEDLGETGKRGGPGGGHSDRGGDRGGRGSG
                     GGRDGDRGGDRGGRGQGRRGGEGRGGGQSRGPRDAAAGEARAPRDGASTEGKAREGAP
                     KRNRNRRRSGGSRGASSAPAAE"
     gene            complement(2177..2881)
                     /locus_tag="VFC06_07000"
     CDS             complement(2177..2881)
                     /locus_tag="VFC06_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HZL81662.1"
                     /translation="MAGLEEAAETAAAIGINFVPGIEVSCTYHGVSVHLLAYWPDPSH
                     PDIVEMLDRTRTARVARAREMVALIGVDYSLTWDAVVEHSGDAETIGRPHIADALVAA
                     GAFATRDEAFIEVLAGNSAYFVPHYAPPVLDAIRTFRLTGAVPVMAHPGADGRGRVVP
                     TSAIEEMARAGLVGLEVEHRDHTEMQRGRLARIAERLDLVHTGSSDYHGAGKLNQLGE
                     NVTSPAALEALRLLRG"
BASE COUNT          518 a         1066 c          998 g          459 t
ORIGIN      
        1 cgcgctcctc aatcaccgat tgcgttgcgg tgaggtgcag gaacatggtg ggaccggccg
       61 cctctcgaat ccggttgcga tacgagcgcc gcaacgccga acacgccacc accacgtccg
      121 tctggggttt catccacgcg cccacggcgt ccaaccacgg ccaccgttcc tcgtcggtga
      181 ggggaatccc cttggccatg gcggccacgc tggcgggtgg gtggaagtca tcagcgtccg
      241 cgaactcgta gcccatctcg gcggcaagcg cggcacccac tgaggacttg ccgcagccgg
      301 tgacacccat cacaatcacg cgcatgccgc cagcctagac tccccggcgc gcgccgtccg
      361 agcgtccaca agccgccacc cgctagacta ggagcgacca catggttgac cggtcgcatg
      421 tgatgcgcgg cgtgacgccg cttttttacg ataatgcgcg atcggctact agcccgcgac
      481 tcaacgtcgc cccgcatttg aggaccacat gactaccgag acttttgccg attttggcgt
      541 gaacccactc atcgtggacg cactcgtcga ccacggcatc attgccccct tccccatcca
      601 gtcgatgact cttcccgtgg cgctttccgg ccacgacatc atcggccagg ccaagaccgg
      661 taccggcaag acgctcggtt ttggcgttcc gttgctgcac cgtgcggtgt cgccaggaga
      721 gcccggcttt gacgacatgg gcgacgcctc gggcaagccc caggcgctcg tcgtcgcgcc
      781 cacccgcgag ttggcagtgc aggtcgccaa cgacctggag atggcctcca agaagcgctc
      841 gacccgcatc ctgaagatct acggcggccg cgcctacgag ccccagatcg acgcgctccg
      901 caagggcgtc gaggtcgtgg tcggcacccc cggacgcctc atcgacctca tgaaccaggg
      961 ccacctggac ctgcgctacg tgcgcaccgt cgtgctcgac gaggccgacg agatgctcga
     1021 ccttggcttc ctcggcgatg tcgagaagct acttgcttcg acccccgctg gccgccacac
     1081 catgctgttc tcggcgacga tgcccggccc cgtggttgcg ctggcccgcc gctacatgac
     1141 gcagcccact cacattcgcg cctccgagcc ggatgacgac ggcgccaccg tcaaggcgac
     1201 cgcacagttc gtgtaccgcg cccacgcgct cgacaagatc gaggtgctcg cccgcatcct
     1261 gcaagcggaa ggccgcggac tcacgatcgt gttcgcccgc accaagcgca cgtgcgccaa
     1321 ggtcagcgat gaccttcgcg accgcggctt cgcggcaggc gcccttcacg gcgacctcgg
     1381 ccagggggcc cgcgagcagg cgctgcgcgc cttccgtgcg ggaaagattg acgtgctggt
     1441 cgcgaccgac gttgcggccc gcggcatcga cgtcgaggac gtcacgcacg tgatcaacta
     1501 ccagtgcccc gaggacgaga agacgtacgt tcaccgcatc ggccgcacgg gccgcgcggg
     1561 caagaagggt gtggccgtca cattcgttga ctgggacgac atgcctcgtt ggggcatgat
     1621 cgaccgctcg ctcgacctgg gataccccga gcccgaagag acgtactcca attcacctca
     1681 cctctacgcg gagctcagca tcctcactgg caagaagggc actctcccgc gctcgtcgcg
     1741 ggttcgcgag ggcctcgatg ccgaaaaggt cgaggacctg ggcgagacgg gcaagcgcgg
     1801 cggccccggc ggcggccatt cggatcgtgg cggcgaccgt ggcggacgcg gctctggcgg
     1861 cggacgcgac ggagatcgtg gtggagatcg tggcggacgc ggacagggac gacgcggcgg
     1921 cgaaggccgt ggcggcggac agtctcgcgg gccccgcgac gcggcagcgg gcgaggctcg
     1981 ggccccgcgc gacggcgcct cgaccgaggg gaaggctcgc gagggcgccc cgaagcgcaa
     2041 ccgcaaccgc cgccgttccg gcggcagtcg gggagcctcg tcggctcccg ccgccgagta
     2101 gccttcacga ctcggagtag tcagaacgac gcagcgcagt cagaacgacg cagcgcagtc
     2161 caaacaactc ggagcgttag cctctgagca acctcagcgc ctcaagcgct gcgggcgacg
     2221 tgacgttctc tcccaactga ttgagcttgc cggcgccgtg atagtcggac gagccggtgt
     2281 gcaccaaatc cagcctctcg gcaatgcgcg ccaggcgtcc tcgttgcatc tctgtgtggt
     2341 cccgatgttc tacctcgaga cccaccaggc ctgcgcgcgc catctcctcg atggccgacg
     2401 tgggcacgac gcggcccctg ccgtcggcac cgggatgcgc catgaccggt acggctccgg
     2461 tgaggcggaa cgtgcggatc gcgtccagta ccggcggcgc gtagtgcggc acaaagtagg
     2521 ccgagtttcc cgccagcacc tcgatgaagg cttcgtccct ggtggcgaac gcccccgccg
     2581 cgaccaaagc atcggcgatg tggggtcgcc cgatcgtctc ggcatcgccg gagtgctcca
     2641 cgaccgcgtc ccaggtgagc gagtagtcga cgccgatcag cgcgaccatc tcgcgtgccc
     2701 tggcgacccg cgccgtgcgg gttctgtcga gcatctccac gatgtcgggg tgcgacgggt
     2761 ctggccagta cgcgagcagg tgcacagaga cgccgtgata cgtgcacgag acctcgatgc
     2821 ccggcacaaa gttgatgccg atggccgcag ccgtctctgc ggcctcctcg aggccagcca
     2881 tggagtcatg gtcggtgagg gcaaccacgt cgaggccagc gacgtccgcg gcacggagtc
     2941 gccctcagag gtagtccgtg ccgtcggaga cgtaggagtg ggtatggagg tcgatgagca
     3001 cgcagcaagc ctacggtgcg gcatggcaac cgatgcaccc g
//
LOCUS       DASRKH010000436         2055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_11960, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000436 DASRKH010000000
VERSION     DASRKH010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11960"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="VFC06_07005"
     CDS             <1..714
                     /locus_tag="VFC06_07005"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HZL81663.1"
                     /translation="SESVANNAVRRFHDELKWADEVFSLFKDDSYLSRLNRGEISVDD
                     CPEEMFEILNACEWYRAKTLGGFDARRPDHIDPTGIVKGWAVARGSAAIDAVGAASWM
                     IGASGDVLVSGEGQQWRIGIADPRVSGDPTGRPVVDVVMLGGKFRALATSGSAQKGLH
                     IWDPSTGAGADHYLSVSVMADNMVDADAWATAIAAGGEKVLSAALDSGMEAMAITTVR
                     PDGTYGAYASSGWPSILPA"
     gene            869..1468
                     /gene="rpsP"
                     /locus_tag="VFC06_07010"
     CDS             869..1468
                     /gene="rpsP"
                     /locus_tag="VFC06_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010145122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HZL81664.1"
                     /translation="MAAKIRLKRFGKKRAPYYRVVVMDSRAKRDGRAIEEIGKYHPTE
                     EPSVIVIDSERVQYWLSVGAQPSDQVRALLKLTGDWGIFKGDKDAKSTVKFAEGKVNP
                     EDAIKAHADQAEKLKAAKATAKEEAAKEAEVAAKEAAKEAEVAAKAEADAKPAADVVA
                     DESSEVPTDEGTDVDVEIAEGDPSDTDDKVEGSLDEEAK"
     gene            1472..1702
                     /locus_tag="VFC06_07015"
     CDS             1472..1702
                     /locus_tag="VFC06_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HZL81665.1"
                     /translation="MVLEALEFLVRAIVRNPEDVRVTERSGRRGVTLNVVVHPDDLPR
                     VIGRHGRTANSLRTVVDAVSREDVRVNIVDVD"
BASE COUNT          361 a          668 c          694 g          332 t
ORIGIN      
        1 tccgagagcg tggccaacaa cgcggtgcga cgcttccatg acgaactcaa gtgggcagac
       61 gaggtcttct cgctcttcaa ggacgattcc tacttgtcgc ggctcaatcg cggcgagatc
      121 tcggtggacg actgcccgga agagatgttc gagatcctga acgcgtgcga gtggtaccgc
      181 gccaagactc tgggaggctt cgacgcgcgc cgccccgacc acatcgaccc gacaggaatc
      241 gtcaagggtt gggccgtcgc aaggggttcc gcagcgatcg acgccgtggg tgcggcctcc
      301 tggatgattg gcgcgagcgg cgacgtcctg gtgtcaggag agggccaaca gtggcgcatc
      361 ggtatcgccg acccccgcgt cagcggcgat ccgaccggac gccccgtggt ggatgtggtg
      421 atgctaggcg gcaagttcag ggcgcttgcc acgtcgggga gcgcgcagaa gggccttcac
      481 atttgggatc cctcgacggg ggcgggcgcc gaccactacc tgtcggtgtc cgtgatggcg
      541 gacaacatgg tcgacgccga tgcgtgggcc acggcgatcg ctgcgggcgg cgaaaaggtg
      601 ctgtcggccg cgctggactc cggcatggag gccatggcta tcaccaccgt gcgtcccgat
      661 ggaacctacg gcgcctacgc atcgtccggc tggccatcga tcctgcccgc gtagcgttcg
      721 cgcaccgggc ccgcactctg gcatacttgt ccgttggcgc tcgcggccct ctacccgctg
      781 gtgaccattc gtcttgtcag gcgacccgat tcatttcccg gatcgacgcc gcccgactca
      841 caccgaatgc ataggagcag taccgaaaat ggcagccaag atccgcctca agcgctttgg
      901 caagaagcgc gccccctatt accgcgtcgt cgtcatggac tcgcgcgcca agcgcgatgg
      961 tcgtgccatc gaagagatcg gcaagtacca ccccaccgag gagccctcgg tcatcgtgat
     1021 cgactccgag cgggtccaat actggctttc ggtcggtgca cagccgtccg accaggtgcg
     1081 cgcgctgctg aagctcacgg gcgactgggg catcttcaag ggagacaagg acgcgaagag
     1141 caccgtcaag tttgccgagg gcaaggtcaa ccccgaggac gccatcaagg cgcacgcaga
     1201 ccaggccgag aagctgaagg ccgcgaaggc caccgccaag gaggaggctg ccaaggaggc
     1261 cgaagtggcc gccaaggagg ccgccaagga ggccgaagtg gccgccaagg ctgaggccga
     1321 cgcgaagccc gcagccgacg tggttgctga cgagtcctcc gaggttccca ccgatgaggg
     1381 caccgacgtt gacgtcgaga tcgcagaggg tgaccccagc gacaccgacg acaaggtcga
     1441 gggttccctc gacgaggagg cgaagtaacg catggtcctt gaggccctgg agttcctggt
     1501 tcgtgcgatc gtccgcaacc cggaagacgt gcgtgtcact gagcgttctg ggcgtcgcgg
     1561 cgtgaccctc aatgtggtgg tccacccgga cgaccttccc cgagtcatcg gccgtcacgg
     1621 ccgcacggcg aactcgctgc gcaccgtcgt cgacgccgtt tcgcgcgaag acgttcgcgt
     1681 caacatcgtg gatgtcgact aagttccaag cagtgcaaaa ggtccggtcc cttttgaggg
     1741 gccgggcctt ttgcgttgcc cctcgagccg ccgccctccc gagactccgc cctcgacgct
     1801 ccgccccgac gctccgaacc tttcaggatg gcgacacgcc gcgtgacgcg gtgggcgctc
     1861 tgcgagggcg gagtgttggg tgcgcgaaag gttcgtagcg tcgggtgacg ccttgaccgg
     1921 gcttccgggg acctgcttcc cgtcgccctg gtcacttctg accgcgacgg ggcgcgacac
     1981 gcggtctttg gcccgcgagg ccgaagtgtc gcggcgtcac gcggtcagaa ctctcccacc
     2041 gtggagagtg cgcgc
//
LOCUS       DASRKH010000437         2510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_10817, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000437 DASRKH010000000
VERSION     DASRKH010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10817"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="VFC06_07020"
     CDS             complement(<1..451)
                     /locus_tag="VFC06_07020"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HZL81666.1"
                     /translation="MALLTAEDVLNKAFSKTKYREGFDQDEVDDFLDEVANTIAALTA
                     ERDELQRRLAQSPEDRAAAPAAAQAPASAAMPGAETAQSPSRSLLQAATDPNPPSATT
                     MLAMAQKLHDEYVQAGEEARDRLVDEGKVKAETIVEQAEKDARGRLEG"
     gene            complement(553..855)
                     /locus_tag="VFC06_07025"
     CDS             complement(553..855)
                     /locus_tag="VFC06_07025"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HZL81667.1"
                     /translation="MIFAIELALFALLIIMLMLLARIALSWVFTFARDWQPRGFSALM
                     VESVYVVTDPLIKPLRRVLPPLTIGMVRIDVAFLLVFFAVIILRSVLTQVLYNLGG"
     gene            complement(904..1380)
                     /gene="sepF"
                     /locus_tag="VFC06_07030"
     CDS             complement(904..1380)
                     /gene="sepF"
                     /locus_tag="VFC06_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HZL81668.1"
                     /translation="MSAMRKAMQYFGFEVADRDDYDYDYADEELAPVTNLHDVSHVRE
                     QHDSRPRAAAVRPQRPQPAEHGDLRRIQTIKPRAYSDARLIGEAFRQGVPVIMNLTEM
                     TDAEAKRLVDFSAGLSFGLHGNIERVTTAVFLLSPAHVEIAVDSAPPVADSGFFSN"
     gene            1722..>2510
                     /locus_tag="VFC06_07035"
     CDS             1722..>2510
                     /locus_tag="VFC06_07035"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HZL81669.1"
                     /translation="MALLTAEDVLNKAFSKTKYREGFDQDEVDDFLDEVANTIAALTA
                     ERDELQQRLAQSPEDGAAAQSAASAAMPGAETAQSPSRSLLQAATDPNPPSATTMLAM
                     AQKLHDEYVQAGEEVRDRLVDEGKVKAETIVEQAEKDARGRLEGLETERADLERRIDD
                     LRRFERDYRGHLRNYLENLLGDLEHDTAKASAGPVGSSTEEPTPTPEPEPEPEPESEP
                     ESEPEVAPVFGEPEAAPEAAPEAEPETPWSPLTAFPEPSFPEEPT"
BASE COUNT          487 a          781 c          798 g          444 t
ORIGIN      
        1 ggccctccag acgtccccgc gcgtccttct cggcctgctc gacgatggtc tcggccttga
       61 ccttgccttc gtccaccagg cgatcgcgag cttcctcgcc ggcctgaacg tactcgtcgt
      121 gcaacttctg ggccatcgcg agcatggtcg tagccgacgg ggggttcggg tcggtagcgg
      181 cttgcagcaa ggaccgggac ggcgactgcg cggtctcagc acctggcatt gccgccgatg
      241 ccggcgcctg tgccgcagcg ggagccgctg cgcggtcctc tggggactgt gcgaggcgcc
      301 gctggagctc gtcacgctcg gcggtgaggg cggcaatggt gttcgccacc tcatcaagaa
      361 aatcgtcaac ctcgtcctgg tcgaaaccct cgcggtactt cgtcttcgag aaggccttgt
      421 ttaggacgtc ttcggcggtg agcagtgcca tgcgtcgtcc ctcctcacgt tagggcggag
      481 ccttgtgtcc gccgatgtgt caatattcac gtactccccc tacgtatcgg aaagcacttg
      541 agttccttgc tgctatcccc cgaggttata cagcacctgc gtgagcaccg aacgcaagat
      601 gatcacagcg aagaagacaa gtaagaatgc tacgtcaatt cgcaccattc cgatggtgag
      661 cgggggcaag acgcggcgca acggtttgat gagtggatca gtgacgacgt ataccgactc
      721 caccatgagc gcggaaaacc cgcggggctg ccaatccctc gcgaaagtga agacccacga
      781 caacgcgata cgcgcgagca acatgagcat gatgatgagc agcgcaaaca gcgcaagttc
      841 tatggcgaag atcacgggtt aagggtaacc gagtgccacg gtctgaccgg gcagcgctcg
      901 atgctagttc gagaagaatc cggagtcggc cacggggggc gccgaatcaa cggcaatttc
      961 gacgtgcgcg ggagacagca ggaacacggc cgtcgtgacc cgttcgatgt tgccgtgcag
     1021 tccaaaggac aggcccgcag agaagtccac gagtcgcttc gcctcggcat cggtcatttc
     1081 cgtgaggttc atgatgaccg gaacaccctg acggaacgct tcgccgatga ggcgcgcgtc
     1141 gctgtaggcc cgcggcttga tggtctgaat gcgtcgtaag tcaccgtgtt cggccggctg
     1201 ggggcgctgt ggccgtaccg cagccgcacg cgggcgagaa tcgtgctgtt cccgcacgtg
     1261 agagacatca tgcagatttg tcacgggcgc gagctcctca tcggcatagt cgtagtcgta
     1321 gtcgtcacgg tccgcgactt cgaaaccgaa gtactgcatc gccttgcgca ttgcgctcat
     1381 ggtttcgctc cgctcttgct tgctggatcc tgcatcctca acggtaggtt gtccggggct
     1441 tgctggcccg catcgacacg cgggccggtc cacgtggtca cgacggctgc ccttcacaca
     1501 cgatgacccc cgctcaccat cggaatggtg cgacttgacg tagcattcct actggtgttc
     1561 tttgccgtga tcatcttgcg ttcggtgctc acgcaggtgc tgtataacct cgggggatag
     1621 cagcaaggaa ctcaagtgct ttccgatacg tagggggagt acgagaatat tgacacatcg
     1681 gcggacacaa ggctccgccc taacgtgagg agggacgacg catggcacta ctcaccgccg
     1741 aagacgtcct gaacaaggcc ttttcgaaga cgaagtaccg cgagggtttc gaccaggacg
     1801 aggttgacga tttcctcgat gaggtggcga ataccattgc cgccctcacc gccgagcgtg
     1861 acgagctcca gcagcgcctg gcgcagtccc cagaggacgg cgcagcggca cagtcggcgg
     1921 catcggcggc aatgccaggt gctgagaccg cgcagtcgcc gtcccggtcc ttgctgcaag
     1981 cggctaccga cccgaacccc ccgtcggcta ccaccatgct cgcgatggca cagaagctgc
     2041 acgacgagta cgttcaggcc ggcgaggaag ttcgcgatcg cctggtggac gaaggcaagg
     2101 tcaaggccga gaccatcgtc gagcaggccg agaaggacgc gcggggacgt ctggagggcc
     2161 tcgagaccga acgcgccgac ctggagcgcc gcatcgacga cctgcgccgt ttcgagcgcg
     2221 actatcgcgg tcacttgcgc aactacctcg agaacctgct gggcgatctt gagcacgaca
     2281 ctgcgaaggc gagcgcaggc cccgtgggtt cgtccaccga agaaccgacg ccgacgcccg
     2341 agcccgagcc cgagcccgag ccggagagtg agccggagag tgagcctgaa gttgcaccgg
     2401 tgttcggtga accagaagcc gcaccagagg ccgcaccaga ggccgagccc gagactcctt
     2461 ggtcgccact gactgctttt cccgagccgt cgttccccga ggagccgact
//
LOCUS       DASRKH010000438         2713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_34046, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000438 DASRKH010000000
VERSION     DASRKH010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34046"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..284)
                     /locus_tag="VFC06_07040"
     CDS             complement(159..284)
                     /locus_tag="VFC06_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81670.1"
                     /translation="MSHGLPTGGPDVAGDVVLAGKGLGDGLGVGDVVAVKEGDGL"
     gene            283..795
                     /locus_tag="VFC06_07045"
     CDS             283..795
                     /locus_tag="VFC06_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81671.1"
                     /translation="MPGASTAGLPAIDFNRYVDICLGMSFSEASAAMPGPAVAGEAMC
                     PWYAELLSVDPPGLYVAAVTYPENPGDEIFLFRMIWTGNPADAASFAAPATQAGISVG
                     ATTAQVNAAYPTATAVTIDDPARGPRNQLVVAGPAGNSIVFDVTSGRVDAMYWGKRIA
                     SGAAGELCAL"
     gene            899..2002
                     /locus_tag="VFC06_07050"
     CDS             899..2002
                     /locus_tag="VFC06_07050"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZL81672.1"
                     /translation="MRILESWHREITLLEASVRVLFIGGTGLISSAASPLLVERGHDL
                     TLVTRGTSAKAAVPAGAATIVTDVHDTEAFRAAIKADVSAHGRYDVVVQWIGFSPDHI
                     SDDIETFDGITDQYVFISSASAYATPPQHYIVREDSTPLHNPFWQYSRDKAEAERRLR
                     VAYGERGFPFTVVRPSHTYGYADVPMAINSWVHPWTMVDRIKTGQKILMPGDGSNLWT
                     LTDHRDFAYAFVGLLGQQRAIGEEFHITSDDVLSWNQVHEIVARAVGVTPEQLHDQTL
                     FIPSQLLARFDREAFEGPLLGDKANAGIFDNSKVRSIVPDYVPQHRFEQSIHESIVWF
                     MGRPERRAIDTAAAALWDHVAERYSRGVDAMFS"
     gene            complement(2050..>2713)
                     /locus_tag="VFC06_07055"
     CDS             complement(2050..>2713)
                     /locus_tag="VFC06_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HZL81673.1"
                     /translation="ITTALSEGISVNVTLIFSLDRYREVVEAFLSGLEKADAAGHDVS
                     TIVSVASFFVSRIDSKVDGLLDDMGPGHPARGTAAIASARLAMEFHEQTLASDRFKAL
                     AAKGAHAQRPLWASTSVKDDAYPDTMYVDELVTHGVVNTMPMSTMQAVADHGQTPPDP
                     TNWDTVRGTYEVARSALAAIEQAGVSMADVTAELEDEGVDKFVASWEELLDHVSAAMS
                     AH"
BASE COUNT          463 a          959 c          841 g          450 t
ORIGIN      
        1 gagacagacc gcaccatgcg cgcggtcgac atgcgggcca acgcaccgct gtgccacgct
       61 gtgcccatgc gccgtaccag catcgtcgtg ttcgcagtag ccgcagccct cgcgctcggc
      121 gcctgctcgt ccagcacgcc cgacctgacg gcaacgactc acagcccgtc gccctccttc
      181 acggcgacca catcgccgac cccgagcccg tcgccgagcc ccttgccagc gagcaccacg
      241 tctcctgcga cgtctggccc gcccgtcggc aacccgtgcg acatgccggg agcgtccacg
      301 gccggactgc ccgcgatcga cttcaaccgc tacgtcgaca tctgtttggg catgtcattc
      361 agcgaagcgt cagccgcgat gcccggcccc gcggtcgcgg gagaagcaat gtgcccctgg
      421 tatgcggagc tgctcagcgt cgaccctccc gggctctacg tcgcagcagt gacgtacccc
      481 gagaacccag gtgacgagat cttcctgttc cggatgatct ggaccggcaa cccggcggac
      541 gccgcttcct tcgcggcccc tgccacacag gcagggattt cggtcggcgc cacgaccgcg
      601 caggtcaacg cggcgtaccc cacggccacc gccgtcacga tcgatgaccc ggcacgtggg
      661 ccacgaaacc aactcgtcgt cgctggcccg gccggaaact ccatcgtctt cgacgtcacg
      721 agcggacgcg tcgacgccat gtactgggga aagcgaatcg cgtcgggagc cgccggcgaa
      781 ttgtgtgccc tgtgaccccg tgcacacctg ctgcgcgacc tcgctagacg gcgggccgcg
      841 ttgcagggac cctctcaatg acaccgaaat tgggggcata gactgacgca gagcgggctt
      901 gagaatcctc gagagctggc accgcgaaat cacacttctg gaggcgtcgg tgcgggttct
      961 cttcattggc ggcacgggtc tcatctcctc agccgcctcg cccctcttgg tggaacgcgg
     1021 ccacgatctc acgctcgtca caaggggcac gtcggcgaag gcggccgttc cggccggagc
     1081 cgccacgatc gtgacggatg tgcacgacac cgaggccttc cgcgccgcca tcaaggccga
     1141 cgtctccgcc cacggtcgat acgacgtcgt ggtgcagtgg attggcttct cccctgatca
     1201 catcagtgac gacatcgaga cctttgacgg catcacggat cagtacgtat tcatctcgtc
     1261 ggcgtccgca tatgccaccc caccccagca ctacatcgtg cgcgaagact ccacgccact
     1321 acacaacccg ttctggcaat acagcaggga taaggcggag gccgaacgac gcctgcgcgt
     1381 cgcctacggt gagcgcggct tcccgttcac cgtggtgcga ccctcacaca cctacggcta
     1441 cgcggacgtg cccatggcga tcaactcgtg ggtgcacccg tggaccatgg tggaccgcat
     1501 caagaccggc cagaagatcc tgatgcctgg cgacggctcg aacctgtgga cgctcaccga
     1561 ccatcgcgac ttcgcctacg cctttgtggg cctgctgggt cagcagcgcg ccatcggcga
     1621 ggagttccac atcacgtcgg acgatgtgct cagctggaat caggtacacg agatcgtggc
     1681 gcgcgccgtc ggcgtcaccc ccgagcagtt gcacgaccag accctcttca tcccgagtca
     1741 gctgctcgcg cggttcgatc gcgaggcgtt cgagggcccg ctgttgggcg acaaggccaa
     1801 cgcgggcatc ttcgacaact cgaaggtgcg gtcgatcgtg ccggactatg tcccgcagca
     1861 ccgctttgag cagtcgatcc acgagtccat cgtgtggttc atgggccggc ccgaacggcg
     1921 cgccatcgac accgccgccg ccgcgctgtg ggaccacgta gccgagcgct actcccgcgg
     1981 cgtggacgcg atgttttctt gatggcgcag ttctcctaga agtacgcgcc cacccgcggg
     2041 ctgccgcgcc tagtgagcgg acattgccgc gctcacgtgg tcgaggagtt cctcccagga
     2101 cgccacaaac ttgtcgacgc cctcgtcttc gagctcggcc gtcacgtcgg ccatcgagac
     2161 ccccgcctgc tcgatcgcag ccagcgctga tcgcgcaacc tcgtacgtac cgcgcacggt
     2221 gtcccagttc gtgggatccg gcggggtctg gccgtgatcg gccacggcct gcatcgtgga
     2281 catcggcatc gtgttgacca cgccgtgagt gaccaactcg tcgacataca tcgtgtcggg
     2341 gtacgcgtcg tccttgacag aggtcgacgc ccacagcggc cgctgagcgt gggctccctt
     2401 ggccgcgagc gccttgaatc ggtcggaggc cagagtctgc tcgtggaact ccatggccag
     2461 gcgggcactc gcgatcgctg ccgtcccgcg ggccgggtgg cctggaccca tgtcgtcgag
     2521 cagcccgtcc accttggagt caatgcgact gacgaagaac gacgcgaccg acacaattgt
     2581 cgacacgtcg tggccggcgg catcggcctt ctcgaggccg gacaggaacg cttcgacgac
     2641 ctcgcggtac cggtcgagcg agaagatcaa ggtgacgttg acgctgatgc cctccgagag
     2701 cgccgtggtg atg
//
LOCUS       DASRKH010000439         2283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_8228, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000439 DASRKH010000000
VERSION     DASRKH010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8228"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..568
                     /locus_tag="VFC06_07060"
                     /pseudo
     CDS             1..568
                     /locus_tag="VFC06_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888191.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
     gene            complement(581..1966)
                     /locus_tag="VFC06_07065"
     CDS             complement(581..1966)
                     /locus_tag="VFC06_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015884008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HZL81674.1"
                     /translation="MSAPSVSSSITARLEVLARPTAVSELTTAIEREGGIVTALDVTA
                     SGHEHMTVDVTCATRGEEHAAQIVDALRVLPGVVVDRVSDRTFLLHLGGKLSIESKVP
                     LRNRDDLSMAYTPGVARICLAIAAHPEDARRLTIKRNTIAVVTDGTAVLGLGDLGPLA
                     ALPVMEGKAVLFKRFAGIDAFPIALDTTDVDQIVETVCAIAPVFAGINLEDISAPRCF
                     EIERRLRERLDIPVFHDDQHGTAIVVVAALTNALKVVGKEIGTVRIVLSGAGAAGTAV
                     LKLLIAAGARDIVVADIDGVVHSGRPGLAPSLVWTAEHTNPRGVSGRLRAALAGADVF
                     IGVSAPDILTGDDIATMAADSIVFALANPRPEVDPDEAALHAAVVGTGRSDFANQINN
                     VLAFPGVFRGLLDSRSHKVTEALLLAAARALASVVTDAELNPTYIVPSVFHPDVTAVV
                     AAAVAEAAQGR"
     gene            1938..2105
                     /locus_tag="VFC06_07070"
     CDS             1938..2105
                     /locus_tag="VFC06_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81675.1"
                     /translation="MDDETLGADMPLSVARRRLIGAQASRFVVVVDAPYGRPDRCEHL
                     ERLRWMSRQST"
BASE COUNT          298 a          826 c          850 g          309 t
ORIGIN      
        1 gaccacggtc ggcccgtccg cgctcgcgtt ccccgggcgc gccgcacccg acgccctgag
       61 caccgcccag atcgccgacc tgcccggcgc gttcgccgcg gctgcccggc gcgcgctggc
      121 cgcagggttc gacgtcgtcg agctgcgcgc cgcgcacggc tacctgctcc acgagttcct
      181 ctcgccgctg tcgaacacgc ggaccgacga gtacggcggc tcgctcgaga accgcgcccg
      241 gctgctcgtc gaggtcaccg acgcggtccg cacggtgtgg cccgacgacc gcccgctgtt
      301 cgtgcgcgtc tccgcgaccg actggaccga cggcggcctg accgtcgacg aggtcaccca
      361 ggtcgcgaaa gagctcggtg cgcacggcgt cgacctcgtc gacgtctcga cgggcggcaa
      421 cgtccccgcg gaggtcccgg tcggcccggg ctaccaggtg cccgccgcac gtgccgtccg
      481 ggccgggtcc gggctgccgg tcaccgcggt cgggctcatc accgacgcgc agcaggccga
      541 gcagctgctc gtcgacggtg cggccgaccc ggcacgccgg ctaccgaccc tgtgcggcct
      601 cggcgaccgc ggcggcgacg accgccgtga cgtcggggtg gaacacgctc ggcacgatgt
      661 aggtcgggtt gagctcggca tccgtgacga ccgacgcgag cgcgcgcgcc gccgcgagca
      721 gcagcgcctc ggtgaccttg tgcgaccgcg agtcgagcag cccgcggaac acgccgggga
      781 acgcgaggac gttgttgatc tggttcgcga agtcgctgcg gcccgtcccc acgacggccg
      841 cgtgcagcgc cgcctcgtcg gggtcgacct cgggtcgagg gttcgcgagc gcgaagacga
      901 tcgagtccgc cgccatcgtc gcgatgtcgt cgccggtgag gatgtccggc gcgctcaccc
      961 cgatgaacac gtcggcgccc gcgagcgcgg cgcgtagtct ccccgagacc ccgcgcgggt
     1021 tggtgtgctc ggcggtccac acgagcgagg gtgcgagccc ggggcggccg gagtggacca
     1081 cgccgtcgat gtccgcgacc acgatgtcgc gcgcgcccgc ggcgatcagc agcttgagga
     1141 cggccgtgcc cgcggccccc gcaccggaca gcacgatccg gaccgtgccg atctccttgc
     1201 cgaccacctt gagcgcgttc gtgagcgcgg cgaccacgac gatcgcggtc ccgtgctggt
     1261 cgtcgtggaa gacggggatg tcgagccgct cgcgcaggcg ccgttcgatc tcgaagcagc
     1321 gcggtgcgga gatgtcctcg aggttgatcc ccgcgaacac gggcgcgatc gcgcacaccg
     1381 tctcgacgat ctggtcgacg tcggtcgtgt cgagcgcgat ggggaacgcg tcgatgcccg
     1441 cgaaccgctt gaagaggacc gccttgccct ccattacggg cagggccgcg agcgggccca
     1501 ggtcaccgag cccgagcacg gcggtgccgt ccgtcaccac ggcgatcgtg ttccgcttga
     1561 tcgtcaggcg tcgcgcgtcc tcggggtggg ccgcgatggc caggcagatg cgcgcgacgc
     1621 cgggcgtgta ggccatcgac aggtcgtcgc ggttgcgcag cggcaccttc gactcgatcg
     1681 agagcttccc gccgaggtgc aggaggaagg tgcggtcgct gacgcggtcc acgacgacgc
     1741 ccggcaggac acggagcgcg tcgacgatct gtgcggcgtg ctcctcgccg cgcgtcgcgc
     1801 acgtcacgtc gacggtcatg tgctcgtgcc ccgacgccgt gacgtcgagc gctgtgacga
     1861 tcccgccctc gcgctcgatc gcggtcgtga gctcgctaac ggcggtgggg cgtgcgagca
     1921 cctcgaggcg cgcggtgatg gacgacgaga cgctgggggc cgacatgccg ctcagtgtcg
     1981 cgcgccgacg gctgatcggc gcgcaggcgt ccaggttcgt cgtcgtcgtc gacgcgccgt
     2041 acggtcggcc cgaccgctgc gagcacctcg aacggctccg ctggatgtcc cggcagtcga
     2101 catagggtga cggcgtgacg gcggcagggt tccagggtcc ggccacggac gacgccgcgc
     2161 tcgcagcgat cgacggcatc gctcagctgc caggcgtgcc cgaggctgtc gaggccgcgc
     2221 gggtcgcgtg cgagcagctc cgatggcacg aggcgtaccg gcgccggtgg cgcgaggtcc
     2281 ggc
//
LOCUS       DASRKH010000440         2027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_6971, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000440 DASRKH010000000
VERSION     DASRKH010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6971"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..350
                     /gene="rpsT"
                     /locus_tag="VFC06_07075"
     CDS             90..350
                     /gene="rpsT"
                     /locus_tag="VFC06_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HZL81676.1"
                     /translation="MANIKSKIKRIGTNEKSRLRNVAVKSELKTHVRKVREAIASGDK
                     AAAETALKSAAVKLDKASSKGVIHENQAANRKSALAKQVGQL"
     gene            complement(416..1426)
                     /gene="holA"
                     /locus_tag="VFC06_07080"
     CDS             complement(416..1426)
                     /gene="holA"
                     /locus_tag="VFC06_07080"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HZL81677.1"
                     /translation="MPATKPPRRGNAGAPPWHRALPAPVVLVSGPEALLGSRALTLIV
                     DAAKVRDPETDVHRFEAATYTAGLLRSAASPSLFGGTALVIVEGAESMNDAFLVDALE
                     YVAAPDPEAVVVVRHGGGNRGKRLLDAARAAGVPEYGCPAVTRDSDLVDFASAEFSRA
                     RRTVSAAAVRALVDSVGSDVSQLAAACSQLAMDVDGGIDEAAVAKYYGTRVNATGFAV
                     ADAAVVGDRAKALALVRHALETGTDPVPLVAAIAAKLRTLAKVGAARGRGVDPTRDLG
                     IAPWQVDRARRELRNWDADRLASAIELVAAADHAVKGGDRAPAFTVERLVRQVADLAA
                     DV"
     gene            complement(1431..>2027)
                     /locus_tag="VFC06_07085"
     CDS             complement(1431..>2027)
                     /locus_tag="VFC06_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81678.1"
                     /translation="DHDGAVAEIAQVARIEQAWVSPAAVTAGHDIGTADARAAGAQVT
                     VPSAGQVWAVGDAEMAVLYPASGSSHAATSSEINDASIVLSIRAGPATALAWGDLETA
                     GQAAVASTLSRPVVVDLVKVAHHGSSVQFADLAAAVTARVAAISVGADNSYGHPAEET
                     IALYARYVAAVMTTARCGDIALGADGTVASACPSGVAG"
BASE COUNT          333 a          728 c          674 g          292 t
ORIGIN      
        1 gacaggaacc agccttgctg gtatgctagg acgccgtgtg cgcgccagag cgacgcacga
       61 gaacctgtcg tagacctcaa gggatccccg tggcaaacat caagtccaag atcaagcgca
      121 tcggcacgaa cgaaaagtcg cgcctgcgca acgtcgccgt caagtcggaa ctgaagacgc
      181 acgtgcgcaa ggtgcgcgaa gcgatcgcgt ccggcgacaa ggcagcagcc gagactgcgc
      241 tgaagagcgc cgctgtcaag ctcgacaagg catcctctaa gggtgtcatt cacgagaacc
      301 aggcagccaa ccgcaagtcg gcgctcgcca agcaggtggg gcagctctag agccgctccc
      361 ctgaccgccg aaggccccgc attggcgggg ccttttgctt tgcccgaacg tgccgttaca
      421 cgtcggccgc gaggtccgcg acctgtcgca caagcctctc caccgtgaac gcgggggccc
      481 tgtctccgcc cttgacggcg tgatcggccg cagccaccag ttcgatggcg ctggccaggc
      541 ggtccgcatc ccagttccgc agttcgcggc gtgcgcgatc cacttgccaa ggagcgatac
      601 cgagatccct ggtggggtct actcccctgc cgcgggccgc ccccactttg gccagggtgc
      661 gaagcttggc ggcgatggcg gcgacgagcg ggacgggatc ggttcccgtc tcgagggcgt
      721 ggcgcacgag cgccagcgcc ttcgccctgt cgcccacgac cgcggcatcg gctactgcga
      781 agcccgtggc gttgacccgc gtgccgtagt acttggcgac ggccgcctca tcgatcccac
      841 cgtcgacgtc catggccagc tgggagcacg ccgccgcgag ttgtgacaca tcggaaccga
      901 cggagtcgac cagtgccctc accgccgcgg cactgacagt gcgcctggcc cgcgaaaact
      961 cggctgaggc aaagtcgacc aggtcgctat cgcgcgtgac ggccgggcat ccgtattctg
     1021 gaacccccgc agcgcgtgcc gcatccagca atctcttccc ccggttcccg ccgccgtgtc
     1081 tgacgaccac gaccgcttct gggtccggag ccgcgacgta ttcgagtgcg tccacaagga
     1141 aggcgtcgtt catcgactcc gcgccctcga cgatgacgag cgcggtgccc ccgaacagcg
     1201 aggggctggc ggccgaacgc aggagccccg ccgtgtacgt cgcggcctcg aagcggtgga
     1261 catccgtttc gggatcgcgc accttcgccg catccacgat cagtgtcagc gcgcggcttc
     1321 cgagaagcgc ctcaggcccg gacaccagca ccactggcgc cggcagggca cgatgccacg
     1381 gaggcgcgcc cgcgttgccg cggcggggtg gcttggtcgc tggcacggca ctagcctgcc
     1441 acgcccgagg gacacgccga cgccaccgtg ccgtccgcac cgagtgcgat gtcgccacaa
     1501 cgggccgtcg tcatcacggc agcgacgtac ctcgcgtaca gcgcgatcgt ctcctcggcc
     1561 gggtggccgt acgagttgtc ggcgccgacg ctgatggcgg ccaccctggc ggtcaccgcg
     1621 gccgcgaggt ctgcgaattg gacggacgaa ccgtggtgag cgaccttcac caggtcgacg
     1681 accaccgggc gcgacagggt cgatgcgacg gctgcctgcc cagccgtctc caggtctccc
     1741 cacgcgagcg cggtggcggg tcctgcccgg atagacagga cgatgctcgc gtcgttgatc
     1801 tcactcgagg tcgcggcgtg gctcgaaccg gaggcggggt acaggacggc catctcggca
     1861 tcgcccacgg cccacacttg gcccgccgac ggcacggtca cctgggcacc cgccgcacgg
     1921 gcatcggccg tgccgatgtc gtgaccggcc gtcacggccg ctggcgacac ccacgcttgc
     1981 tcaatgcgcg cgacctgcgc gatctcggcg accgcgccgt cgtgatc
//
LOCUS       DASRKH010000441         2966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_8257, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000441 DASRKH010000000
VERSION     DASRKH010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8257"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..1114)
                     /locus_tag="VFC06_07090"
     CDS             complement(125..1114)
                     /locus_tag="VFC06_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZL81679.1"
                     /translation="MSTETAAPRPASVRTRGLLTRVQTIAGQNGALVALILLWTVMSL
                     LSPVFFTAQNMLNVGIQAAVVAILAFGQTFVIVSAGIDLSVGSVAALAAVVTGYGATM
                     MGLNPVVAILFGMITGILAGLVSGVLVSYGRLPAFIATMAMLSIARGLALVLSQGVPL
                     AQPSAVAWFGKTVGGWLPMPVVIMVVLGVLASFILGRTYTGRAMYAIGGNEEAARLSG
                     INVRRQKLIIYALSGLFAAVAGLVLAGRLVSAQPQAASGYELDSIAAVVIGGASLAGG
                     TGRAFGTFVGALVLAVIRNGLNLLNVSSFWQQVVIGAVIALAVLTDTLRRRRA"
     gene            complement(1104..2633)
                     /locus_tag="VFC06_07095"
     CDS             complement(1104..2633)
                     /locus_tag="VFC06_07095"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HZL81680.1"
                     /translation="MTESPTAASAGPELLRVEGLVKKFPGVVALGGVSFDLHAGEVHV
                     LLGENGAGKSTLIKCLAGVYQPDGGQVVVDGRPVSITTSARAEALGIATIHQEFNLVP
                     QLSIAENVLLGRQPRRFGLVDKRRMNAEACVAMARVGLDLDPRTPVAGLGVARQQLVE
                     IAKALSLDARVLILDEPTAALTDAEVDRLLELMDELRSHGVGLIFISHHLDEIRRIGD
                     RVTVLRDGNSVGTVPASTSTDELVRMMVGRSIEQQYPRERGDAGDELLRVEHLTSHGR
                     FDDISLSVRAGEVVGIAGLVGAGRTEVLRAIFGADAYSAGSVTVRGKSLPRHDVHAAI
                     GAGLGLVPEDRKAQGLVLSASVGENLGLVALGRATRRGLVNRRWLRQLAADTVDELSI
                     RTPNTQVMVKNLSGGNQQKVVMGKWLSADPAVLLLDEPTRGVDVGARVEIYQLVNKLT
                     SSGRGVLMVSSDLPEVLGMCDRILVMAQGRIVGELTHQEATQDAVMALVVKDVESTRV
                     N"
     gene            complement(2630..>2966)
                     /locus_tag="VFC06_07100"
     CDS             complement(2630..>2966)
                     /locus_tag="VFC06_07100"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZL81681.1"
                     /translation="DALASLLALPEVPDAVFVANGPMALGALQLLGEQQGTSSVRIPD
                     DLALAVFDDMPWFTLLRPTLTAIAQPTSLLGERAMQMLLDRIGGKDVADERIGCRLEL
                     RESTQRRNS"
BASE COUNT          495 a         1000 c         1034 g          437 t
ORIGIN      
        1 ctgacggcgg tgagggtgag gagcttcatg gcgggcttct tcatcgaaac ctttccttcc
       61 tggttggtcc gggagcggtt cccggggtcg tgcctgtgag tggtggcaga ggtgtctgtg
      121 gtggtcaggc ccgacggcgg cgcagggtgt cggtgagcac cgcgagggcg atgaccgcac
      181 cgatgaccac ctgctgccag aacgacgaga cgttcaggag gttcaggccg ttgcggatca
      241 cggcgagcac gagcgcgccg acgaacgtgc cgaacgcccg tcctgtcccg ccggccaggc
      301 tcgctccgcc aatgacgacg gcggcgatcg agtcgagctc gtaccccgag gcggcctgcg
      361 gctgcgcgga gacgaggcgt ccggccagca ccagcccggc gacggccgcg aagagtcccg
      421 agagcgcgta gatgatcagc ttctggcgtc gcacgttgat gcccgaaagg cgcgcggctt
      481 cctcgttgcc gccgatcgcg tacatggcgc ggcctgtgta ggtgcgcccg aggatgaagg
      541 acgcgagcac cccgaggacg accatgatca cgacgggcat cggcaaccat ccaccaacgg
      601 tcttcccgaa ccaggccacc gcgctcggct gcgccagcgg gacaccctgg gacagcacca
      661 gggcaaggcc ccgggcgatg gacagcatcg ccatggtggc gatgaacgcc ggaagccggc
      721 cgtaggagac cagaactccg gagaccagac cggccaggat gccggtgatc atgccgaaca
      781 ggatcgcgac cacggggttc agacccatca tggtggcgcc gtacccggtc acgaccgccg
      841 cgagggctgc gaccgagccc acggacagat cgatgccggc cgagacgatc acgaacgtct
      901 ggccgaaggc gaggatggcc acaacagctg cctggatccc gacgttgagc atgttctgcg
      961 ccgtgaagaa gaccgggctg aggagggaca tcacagtcca gagcaggatc agcgccacca
     1021 aggcaccgtt ctggccggcg atggtctgca cgcgtgtgag gagcccccga gtccgcacgg
     1081 acgcggggcg aggggcagca gtctcagttg acacgggtgg actccacatc tttgacgacc
     1141 agtgccatga cggcgtcctg ggtagcttcc tggtgggtga gctcgccgac gatgcggccc
     1201 tgggccatga cgaggatccg gtcgcacatc ccgaggacct cgggcaggtc ggaggagacc
     1261 atcaggacgc ctcgacccga gctcgtcagc ttgttgacga gctggtagat ctcgaccctg
     1321 gcgccgacgt cgacgccgcg ggtgggctcg tcgagcagca ggaccgcggg gtcggccgag
     1381 agccacttgc ccatgacgac cttctgctga ttgcctccgg agaggttctt gaccatgact
     1441 tgcgtgttcg gggtccggat gctcagctcg tcgacggtgt cggcggccag ctgccgcaac
     1501 cagcgccggt tgacgagccc tcgtctggtc gctcggccga gcgcgaccag cccgaggttc
     1561 tcgccgacgg atgcgctgag gacgagaccc tgggccttgc ggtcttcggg gacgagtccc
     1621 aggcccgcgc cgatggcggc gtggacgtcg tgacgcggga gcgacttgcc ccgcacggtg
     1681 acgctgcccg cgctgtaggc gtcggccccg aagatcgcac ggagcacttc cgttcgcccg
     1741 gcccccacca gaccggcgat tccgacgacc tcacccgcgc gaacgctcag cgagatgtcg
     1801 tcgaaccggc cgtgggacgt caggtgctcg acacgcaaca gctcgtctcc ggcgtcgcca
     1861 cgctcgcgcg ggtactgctg ttcgatggac ctgcccacca tcatccggac gagctcgtcg
     1921 gtggacgtgc tggcaggcac cgtccccacc gagttgccgt cccgaagcac cgtgacccgg
     1981 tcgccgatgc gccggatctc gtccaggtgg tgcgagatga agatcaggcc cacgccgtgc
     2041 gaccgcagct cgtccatcag ctcgagcagc cggtccacct cggcgtcggt cagtgcagcg
     2101 gtcggttcgt cgaggatcag tacgcgggca tccaggctca gcgccttggc gatctcgacg
     2161 agctgttggc gcgccacgcc cagacccgcg acaggagtgc gcgggtcgag atcgaggccg
     2221 acccgagcca tcgcgacgca ggcttcggca ttcatgcgcc gcttgtccac gagcccgaac
     2281 cggcggggct gacgtcccaa gagcacgttc tcggcgatgc tgagctgcgg gacgaggttg
     2341 aactcctggt ggatcgtggc gatcccgaga gcttccgcgc gtgcgctcgt cgtgatgctc
     2401 accggtcggc cgtcgacgac gacctggccg ccgtccggct gatacacccc ggccaggcac
     2461 ttgatcaagg tcgacttccc ggcaccgttc tcgccgagga gcacgtgcac ctcacccgca
     2521 tgcaggtcga acgagacccc gcccagcgcc accacgccgg ggaacttctt gaccaggccc
     2581 tcgacccgca ggagctccgg tccggcggac gcggcagtcg ggctctcggt catgagttcc
     2641 tcctctgggt ggactcacgc agctccagcc ggcagccgat gcgctcgtcc gcgacgtcct
     2701 tgccgccgat ccggtcgagg agcatctgca tggcgcgctc gccgagcagc gaggtcggct
     2761 gggcgatggc ggtgagggta gggcgcagca gcgtgaacca cggcatgtcg tcgaagacag
     2821 ccagggcaag gtcgtccggg atccgcacgc tcgaggtccc ctgttgctca ccgagcagct
     2881 gcaacgctcc gagcgccatg ggcccgttgg cgacgaacac cgcgtccggg acctccggca
     2941 aggcaagcag ggacgcaagg gcgtcg
//
LOCUS       DASRKH010000442         2415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_6996, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000442 DASRKH010000000
VERSION     DASRKH010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6996"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..153)
                     /locus_tag="VFC06_07105"
     CDS             complement(<1..153)
                     /locus_tag="VFC06_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZL81682.1"
                     /translation="MGVIEGGRPRAVTLKDVARIAGVSSSTASKALNGRADVNPETRR
                     RVQETAE"
     gene            325..1620
                     /locus_tag="VFC06_07110"
     CDS             325..1620
                     /locus_tag="VFC06_07110"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZL81683.1"
                     /translation="MKIPAPSPRRTRHATLAGAAVVSALALAACGGGDGGTTSTATGD
                     GATGAAGDQTVTVWHYFSADNQVQLMDEYAATFEAAHEGVTVENVFIPYDQMNSKLVS
                     SAGAETGPDVVVFNGAETAIIALGGVLAPLDDYLGSYEDIDQLPESVLHVIDGTTYAV
                     QGYVNLLGLWYNADILAEIGVEPPTTIDELEAAMAAAKDAGYAGITLCGLPNSQGEWQ
                     AYPWLTAEGFTYENLDEGALAAGLGRVRGWVDNGWLTQEAVTWDQTVPFQTFAAGGVA
                     FAENGNWQMGTAETDATFEYGVVPLPVGDQGKVYLGGEGEGIGAFSENPDLAWEYIAG
                     TYLDPAGQVLAATLVGSIPSRLDAAQDDAIGGNLLLKPFADTIAASGANYPEAVLPPE
                     SVADVQLNVGQAWSATLGGQRSAADAAATAVASLTNLLK"
     gene            1675..>2415
                     /locus_tag="VFC06_07115"
     CDS             1675..>2415
                     /locus_tag="VFC06_07115"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HZL81684.1"
                     /translation="MTTPMAPVTGRVASTTTRPDRRRRKRQHRMLFLLPAAVFLALFS
                     IYPLVQLLRMAFSAVTSATLNAPSWAWVGLDNFAKGFESGDTQHSLRLTVVFVVIVTV
                     LGMLLGLAAAIALRTKGSWSGFLLGLMVFVWALPPVVNGSVWKFLLGDNGLVNMVLTR
                     IGLSEPIPFLYDQYWALMAVAFVNSFAVIPFNALVYRAALMNIETEVFEAAALDGANR
                     WQEIRFIMVPSVRATTLVLLLAADAPGIY"
BASE COUNT          391 a          865 c          749 g          410 t
ORIGIN      
        1 ctcggcggtc tcctgcaccc gtcgtcgtgt ctccggattc acgtcggcac ggccgttcag
       61 ggccttggac gccgtcgatg acgagacacc cgcgattcgc gccacatcct tcaacgtgac
      121 cgcccgcggc cgtcctccct cgattacgcc catccccacc aactccctgc ttcaggtcca
      181 ccgaaagccc agcatagcgg ttttgcttga cagttggaat atgctgttgt agtctataga
      241 aaccggtttc gatacgtttc ggaaccagct cccccccagc cggagacaac gcccgcgacg
      301 acgcggacaa ccgaaggaga cgttgtgaag attccagctc ccagtccacg ccggacgagg
      361 cacgccacgc tcgcaggtgc ggccgtggtc agtgccctcg cactcgccgc ctgcggcggc
      421 ggcgacggcg gcacgaccag cactgccacc ggtgacggcg ccaccggtgc agccggcgac
      481 cagacggtca ccgtgtggca ctacttcagc gcggacaacc aggtccagct catggacgaa
      541 tacgccgcca cgttcgaagc cgcccacgag ggcgtcacgg tcgagaacgt cttcatcccg
      601 tacgaccaga tgaactccaa gctcgtctcc tccgccggcg cggagaccgg acccgacgtc
      661 gtcgtcttca acggcgccga gaccgccatc atcgcgctcg gcggcgtact ggcaccgctc
      721 gacgactacc tcggttccta cgaggacatc gaccagctgc ccgagtccgt gctccacgtc
      781 atcgatggca ccacctacgc ggtgcagggg tacgtcaacc tgcttggcct ctggtacaac
      841 gccgacatcc tcgcggagat cggcgtcgag cccccgacca ccatcgacga actagaggcc
      901 gccatggccg ccgcgaagga cgcgggctac gcggggatca ccctctgcgg gttgccgaac
      961 tcgcagggcg agtggcaggc gtacccctgg ctgactgccg agggcttcac ctacgagaac
     1021 ctggacgagg gcgcgctcgc cgcgggcctc ggccgcgtcc gtggctgggt cgacaacggc
     1081 tggctcaccc aggaggccgt cacgtgggac cagaccgttc ccttccagac gttcgccgcg
     1141 ggtggagtcg ccttcgccga gaacggcaac tggcagatgg gcactgctga gactgacgcg
     1201 acgttcgagt acggcgtcgt ccccctgccg gtcggtgacc agggcaaggt gtacctcggc
     1261 ggcgagggtg agggcatcgg ggcgttctcc gagaaccctg acctcgcgtg ggagtacatc
     1321 gcggggacat acctcgaccc ggcgggtcag gtcctcgcgg ccaccctcgt cggctccatc
     1381 ccgtcccgcc tggacgcggc gcaggacgac gccatcggcg gcaacctgct cctcaagccg
     1441 ttcgccgaca ccatcgccgc ctcgggcgcc aactaccctg aggccgtgct gccgcccgag
     1501 tccgttgccg acgtccagct caacgttggg caggcatgga gtgcaaccct cggcggacag
     1561 cgttcggccg ccgatgccgc cgcaacggcc gtcgcgtcgt tgacgaacct gctcaagtaa
     1621 taccgcacgg tcaccggcgc ccccgggtcg attctcacga aggagtcctc cgctatgaca
     1681 actcccatgg cgccggtgac cggccgtgtc gcgtccacca cgacgcggcc ggaccgccgg
     1741 cgcaggaagc gacagcaccg aatgctgttc ctgctgccgg ccgcggtgtt cctcgcgctg
     1801 ttcagcatct accccctcgt ccagctcctg cggatggcgt tcagcgccgt gacgtcggca
     1861 accctgaacg cgccctcatg ggcgtgggtg ggcctggaca acttcgccaa gggcttcgag
     1921 tccggcgaca cccagcactc gttgcgcctc accgtggtct tcgtggtgat tgtgaccgtc
     1981 ctgggcatgt tgctcggact cgcggcagct atagcgctgc ggacgaaagg cagttggtcc
     2041 gggttcctgc tcggactcat ggtcttcgtc tgggcactgc cgcccgtggt gaacggctcg
     2101 gtctggaagt tcctgctcgg cgacaacggc ctggtcaaca tggtcctcac gcggatcggg
     2161 ctgagtgaac cgatcccgtt cctctacgac cagtactggg ccctgatggc tgtcgcgttc
     2221 gtcaactcgt tcgcggtgat ccccttcaac gccctggtgt atcgcgccgc cctgatgaac
     2281 atcgagacag aggtcttcga agccgccgcg ctcgacggcg cgaaccggtg gcaggagatc
     2341 cgcttcatca tggtcccgtc agtccgggcc accacgcttg tgcttctcct cgctgctgat
     2401 gctccgggca tatac
//
LOCUS       DASRKH010000443         2593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_56991, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000443 DASRKH010000000
VERSION     DASRKH010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56991"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..174)
                     /locus_tag="VFC06_07120"
     CDS             complement(<1..174)
                     /locus_tag="VFC06_07120"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HZL81685.1"
                     /translation="MLKGGIVAYGAEAKHSQLGVSRRLLETDGPVSYAVATSMARGAR
                     RQMGADVAVATTGV"
     gene            130..438
                     /locus_tag="VFC06_07125"
     CDS             130..438
                     /locus_tag="VFC06_07125"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HZL81686.1"
                     /translation="MLRLSAVRNDAALEHGRRPWYVNDRGARRQLGADVAVATTGVAG
                     PDAHGGKPPGTVIVAVVGPQGERVVSLLLDGDRDAVRQGAVAAALSHLEKTLSRLPHS
                     "
     gene            524..850
                     /locus_tag="VFC06_07130"
     CDS             524..850
                     /locus_tag="VFC06_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZL81687.1"
                     /translation="MPVLRNELGDVLRDARLGQGKTLRDISSEARVSLGYLSEVERGQ
                     KEASSELLGSICDALDVPMSAILRDVSERFEMAEGLVDARSLSRMPLMPKIPDTVPEL
                     LHSIEQ"
     gene            857..1090
                     /locus_tag="VFC06_07135"
     CDS             857..1090
                     /locus_tag="VFC06_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010156637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3046 domain-containing protein"
                     /protein_id="HZL81688.1"
                     /translation="MRLSEFRDAVAVAFGEEYAPTLCREMALTSLESLTAQQALAKGI
                     APRDVWHALCDQMDVDPAVRAGQDPRMIIPPRR"
     gene            1294..2382
                     /gene="recA"
                     /locus_tag="VFC06_07140"
     CDS             1294..2382
                     /gene="recA"
                     /locus_tag="VFC06_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HZL81689.1"
                     /translation="MAQAADRSKALEAALGQIDRQFGKGSAMRMGDTERVAVAVIPTG
                     SIALDIALGIGGLPRGRVVEIYGPESSGKTTVALHAVANAQAAGGIAAFIDAEHALDP
                     EYAKKLGVDIDNLIISQPDTGEQALEIADILIRSGGVDVLVIDSVAALVPKAEIEGEM
                     GDSHVGLQARLMSQALRKITGAIANTGTTAIFINQLREKIGVFFGSPETTTGGKALKF
                     YASVRLDIRRIETLKEGTDPVGNRTRVKVVKNKMAPPFKQAEFDIMYGFGISKEGGII
                     DMGVEHGFVKKSGAWYTYEGDQLGQGKENSRQFLKDNPALRDEIEKKIKDKLGVGVVP
                     VPADVAPVPTKADAKALEAALAKEKSSL"
BASE COUNT          472 a          834 c          859 g          428 t
ORIGIN      
        1 taccccggtg gtcgcgaccg cgacatcggc gcccatctgt ctgcgtgcgc cgcgtgccat
       61 cgacgtcgcc accgcgtagc tgaccgggcc gtccgtctca agcagccgcc tgctcacgcc
      121 cagctggctg tgcttcgcct cagcgccgta cgcaacgatg ccgcccttga gcacggccga
      181 cgcccctggt acgtcaacga tcgcggcgca cgcagacagc tgggcgccga tgttgcggtc
      241 gcgaccacag gggtagcggg cccggacgcc cacggcggga agcctccggg gaccgtgatc
      301 gtggccgtgg tgggaccgca aggggagcgc gtcgtcagcc tcctgcttga tggcgacagg
      361 gacgcggtcc gccaaggggc tgtcgccgcc gcgctgtccc acctcgagaa gacgctgtcg
      421 aggttgccac attcgtgaag tccggttcga ttgcgggaac acgcctgcgg tagtctgcgt
      481 tggaagaggt atgcaccaag caagcactat gaaaggagca cgaatgcctg tcctgcgcaa
      541 tgaactcggc gacgtgctcc gcgacgctcg tcttggccaa ggcaagacgc tccgcgatat
      601 ttcctcggag gcccgagtat cactcgggta cctgagcgag gtggagcgcg gccagaagga
      661 agcctcgagc gaacttcttg gctccatctg tgatgcgctc gacgttccta tgtccgcgat
      721 tctgcgcgac gtgtcggagc gtttcgagat ggcagagggc ctggtggatg cacgctcgct
      781 gtcccgcatg cccctgatgc cgaagattcc cgacacggtt ccggaactcc tgcactcgat
      841 cgaacagtag gaacgcttgc ggctttccga gttccgcgac gccgtcgcag ttgctttcgg
      901 ggaggagtac gcacccacgc tatgccgcga aatggcgctc acctcactcg agtccttgac
      961 ggcacagcag gcgctcgcca agggcatcgc gccgcgcgac gtctggcacg cactgtgcga
     1021 tcagatggac gtggaccctg ctgtgcgcgc tggtcaagac ccgcgcatga tcattccgcc
     1081 acgccgatga cggcgtgtcg ccgccattcg aacaggcgtt cgattacact cgtgcccagg
     1141 gcgcccacac ggcgcctctc cccagtagtg gatgtcggat cggctccatg tcagacccgc
     1201 gacgtagcgt cgtagcgtcg tagtcaacga caaggcaccg tgaaggtgtc ccaccgctgg
     1261 caacagtcgg cacgagcgat gaaggacagt catatggcac aggcagcgga ccgctccaag
     1321 gcactcgagg ccgcactggg gcagatcgac aggcagttcg gcaagggctc ggcgatgcgc
     1381 atgggcgaca ccgagcgagt ggcggtggcg gtcatcccca ccggctcgat cgcgctcgac
     1441 atcgcgctcg gcatcggcgg actcccgcgc ggtcgcgtgg tggagatcta cggccccgaa
     1501 tcgtcgggaa agaccacggt ggcgctgcac gcggtcgcca acgcgcaggc cgccggcggt
     1561 atcgctgcct tcatcgacgc cgagcacgcg ctcgaccccg agtacgcgaa gaagcttggc
     1621 gtggacatcg acaacctgat catctcgcag cccgacacgg gcgagcaggc cctcgagatc
     1681 gccgacattc tgatccgttc tggtggggtc gacgttctgg tcatcgactc ggtcgccgcc
     1741 ttggtgccca aggccgagat cgagggcgag atgggcgaca gccacgtcgg cctgcaggcg
     1801 cgcctcatgt cgcaggcgtt gcgcaagatc acgggcgcca tcgccaacac cggcaccacc
     1861 gcgatcttca tcaatcagtt gcgcgagaag atcggcgtgt tcttcggatc gcccgagacg
     1921 acgacgggtg gcaaggcgct gaagttctac gcgtctgtgc ggctggacat tcgtcgaatc
     1981 gagaccctca aggagggaac cgacccggtc ggtaaccgca cgcgagtcaa ggttgtcaag
     2041 aacaagatgg cgcccccctt caagcaggcc gagttcgaca tcatgtacgg attcggtatc
     2101 tccaaagagg gaggcatcat cgacatgggc gtcgagcacg gcttcgtcaa gaagtccggc
     2161 gcttggtata cctacgaggg tgaccaactg ggacagggca aggaaaactc ccgccagttc
     2221 ctgaaggaca acccggcgct gcgcgacgag atcgagaaga agatcaagga caagttgggc
     2281 gtgggtgttg tgcctgttcc ggccgacgtt gccccagtcc cgaccaaggc ggatgccaag
     2341 gctctcgaag ctgctttggc caaggagaag tcctccctct aggaggccgc cgctccacac
     2401 cgcgtcatgt cacctcgcgc tcggcgggag gagccgtcgt gggggcttcc cgccgagcgc
     2461 gaggtgacat gacgcggtgt ggagcggcgg cctcctagag ggaggactgc tccttggcca
     2521 aagccgcttc gagggccttg gcgtccgctt tggtggggac tggtgctgcg tcggccggaa
     2581 caggcaccac acc
//
LOCUS       DASRKH010000444         2048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_37634, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000444 DASRKH010000000
VERSION     DASRKH010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37634"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1283)
                     /locus_tag="VFC06_07145"
     CDS             complement(<1..1283)
                     /locus_tag="VFC06_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HZL81690.1"
                     /translation="MDALFDIASGGSSPLIEGLNPEQAQAVVYDGPALLIFAGAGSGK
                     TRVLTHRIAYLLESGRARPHEVLAITFTNKAAGEMRERVRALVGPEARQMWVSTFHSA
                     CVRMLRRDYELAGLKSTFSIYDTTDSVAVMKLVMKELDIDPKKFTAKALLGRVGNFKD
                     ELIEPDVAAAAGEAASRHSIDNAAGRAYGAYQRRLEAANAVDFDDIIVRTVRLIQNHP
                     EVAAQYRAQFRNVLVDEYQDTNHAQYVLVRELVGDSGNLTVVGDADQSIYAFRGATIR
                     NILEFEKDYPDAEVIRLERNYRSTQNILSAANAVISNNVGRPPKNLWTNAGAGERIIG
                     YAGDTEQDEAVFIADEIRRLTTAENVAPADVAIMYRANSQSRAIEERFIRAEIPYKVV
                     GGTRFDERREIKDMLAYLAAVVNEDDDVATRRVINT"
     gene            complement(1339..2016)
                     /locus_tag="VFC06_07150"
     CDS             complement(1339..2016)
                     /locus_tag="VFC06_07150"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZL81691.1"
                     /translation="MSNRIVAGLCILLVVAVIQVNMSATRRILEGAARYGELRATTDE
                     LTGVYNRRPVVAQLTQWAERGRGNYAIALIDLDHFKTINDEFGHDCGDTVLQEVALTL
                     RSHFRETDMVSRWGGDEFLVLMPGVRHADVVPVLERLRRAIKLIEKRCPEHVHHASVS
                     IGAAMGAMGQSPDECIAAADHALYRAKEEGRDKVVAVGVMEATHAQGRPSPDVSVGTP
                     PEYVNRG"
BASE COUNT          332 a          654 c          674 g          388 t
ORIGIN      
        1 gtgttaatga cgcgcctggt ggcgacgtcg tcgtcctcgt tcaccacggc ggcgaggtag
       61 gccagcatgt ccttgatctc gcggcgctca tcgaaccgcg tgccgcccac caccttgtag
      121 gggatctcag cgcggatgaa ccgctcctcg atggcacgcg actgcgagtt ggcgcgatac
      181 atgatggcga cgtcggcagg ggccacattt tccgccgtgg tgagccgccg gatctcgtcg
      241 gcgatgaaca ctgcctcgtc ttgctccgtg tcgcccgcgt agccgatgat gcgctcgccg
      301 gcgccggcgt tggtccagag gttcttgggc ggcctgccca cgttgttcga gatgaccgcg
      361 ttggccgcgc tcaggatgtt ctgggtcgac ctgtaattgc gctcaagccg gatcacctcg
      421 gcgtccggat agtccttctc gaactccaag atgttgcgga tcgtggcgcc gcggaaggcg
      481 tagatcgact ggtcggcgtc gccgaccacg gtcaagttgc cgctatcccc caccagttcg
      541 cgcaccaaca cgtattgggc atggttggtg tcctggtact cgtcgacaag cacgttcctg
      601 aattgggcac ggtattgcgc cgccacctcg ggatggttct ggatcagccg aacggtcctc
      661 acgatgatgt cgtcaaagtc gaccgcgttg gcggcctcaa gacgacgctg gtaggcgccg
      721 tacgccctgc cagccgcatt gtcgatcgag tgccgggagg ccgcctcccc cgccgctgcg
      781 gcgacgtcgg gctcgatcaa ttcgtccttg aagttcccga cgcggcccag cagcgccttc
      841 gccgtgaact tcttgggatc aatgtcaagc tctttcatca cgagcttcat gacggctacg
      901 gagtcggtgg tgtcgtagat cgagaatgtc gacttgagcc ctgcgagttc gtagtcgcgt
      961 cgcagcatgc gcacgcacgc ggagtggaag gtcgagaccc acatctgcct ggcctcggga
     1021 cccacgagcg cccgaacgcg ttcgcgcatc tctccggcgg ccttattggt gaatgtgatg
     1081 gccagcacct cgtgcggccg ggcgcgtccg gattcgagaa ggtaggcgat ccggtgtgtc
     1141 agtacgcgcg tcttgccgga gccggctccc gcaaagatga ggagtgccgg gccgtcgtag
     1201 acaacggcct gggcctgctc ggggttaaga ccctcaatca ggggggagga gccgccggac
     1261 gcgatgtcga agagtgcatc catggaagca ccagtctagg tcccacggcc gacgcggggg
     1321 tcacgcgagg acggcgcact atcccctgtt gacgtattcc ggcggcgtac cgacggacac
     1381 gtcgggcgac gggcgccctt gagcatgagt tgcctccatc acgcccaccg cgaccacctt
     1441 gtcgcgaccc tcctccttgg cgcggtagag cgcgtggtcg gcggcggcaa tgcactcgtc
     1501 cggcgattgg cccatggcac ccatcgccgc cccaatcgac acgctggcgt ggtggacgtg
     1561 ttcggggcag cgcttctcga tgagcttgat cgcgcggcgc aagcgctcga gaactggcac
     1621 cacgtcggcg tgccggactc cgggcatgag caccaagaac tcgtccccgc cccaacggct
     1681 gaccatgtcg gtctcgcgga agtggctgcg tagcgtcagc gccacctcct gaaggacggt
     1741 gtcaccacag tcgtgaccga actcgtcgtt gatcgtcttg aagtggtcaa gatcgatcag
     1801 cgcgatcgcg tagttgccgc ggccgcgttc ggcccactgg gtcagttgag cgacgacagg
     1861 gcggcggttg tacacgccgg tcaattcgtc ggtggttgca cgcaactcgc cgtaccgggc
     1921 ggcgccctca aggatgcgcc gcgtggccga catgttgact tggattaccg ccaccaccag
     1981 gaggatgcag aggcccgcga cgattcggtt gctcattgcg aaatctctgg cgatggcgcc
     2041 gtcgagct
//
LOCUS       DASRKH010000445         4022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_38412, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000445 DASRKH010000000
VERSION     DASRKH010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38412"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..581)
                     /locus_tag="VFC06_07155"
     CDS             complement(126..581)
                     /locus_tag="VFC06_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HZL81692.1"
                     /translation="MTHPLVGHAAPDFTLSDQDGIEVSLSALRGTDVLLVFVPWAFSP
                     VCTYELEQLRDAEDLVASDAKVFVVNCDSKFVNQEWAYQNKFPGTLLSDFWPHGEVSR
                     AFGMFDEQLGRSKRGTLHIGADGLVTWALENPTGDARDLDAYRKVLGLV"
     gene            complement(578..1036)
                     /locus_tag="VFC06_07160"
     CDS             complement(578..1036)
                     /locus_tag="VFC06_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 family protein"
                     /protein_id="HZL81693.1"
                     /translation="MLTDERGRRAVATTEGSEALDTAMVSRLGFTQEMVVQEFGHDAD
                     VCDALRRAIMSVTGEDLVDEDYGDVTDAAIVWFRDGDDDLADLLMDVQSLIDGSALVV
                     LLTPKARRPGHVLPRDVEEASSLAGLHATSNFLVDETWTAIQLGEKGRSK"
     gene            1263..3977
                     /gene="aceE"
                     /locus_tag="VFC06_07165"
     CDS             1263..3977
                     /gene="aceE"
                     /locus_tag="VFC06_07165"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HZL81694.1"
                     /translation="MAQHGAIDKDPEETNEWLESLDGMIENGGATRAEYVIDRLVERA
                     SFKQLSVPLSLNTPYVNTIHADNEPVYPGNEPFERRYRGWIRWNAAVMVTRAQAAGKG
                     VGGHISSYASVATLYEVGLNHFFKGKDHPGGGDHIYFQGHAAPGVYARGLVEGRFSED
                     DLDSFRQEKSGVGRGLSSYPHPRLMPDLWEFPTVSMGLGPASAIYQAWTDRYLKLRGI
                     KDTSQQRTWAFLGDGEMDEPESRGMLQLAANQKLDNLTFVVNANLQRLDGPVRGNGKI
                     IQELEAFFRGAGWNVIKVIWGRSWDPLLARDHDGALTNLMNTVPDGDFQTFRAESGAF
                     IRDQFFGGDPRAKELVADMTDDEIWALKRGGHDYQKLYAAYSRAAQEKNGKPTVILAK
                     TIKGYGLGTNFAARNSTHQMKKLAAADLKVLRDTFSIPFTDEQIDADPYNPPYYHPGY
                     DDPAIQYMLDRRKDLGGFVPERRDSYDPIELPDPEVYELLAKGSGNHEVATTQAFVRL
                     LKDLIKDKNFGHRIVPIIPDEARTFGLDAIFPSAKIFNTQGQNYLPVDRELMLSYKES
                     ESGQIMHTGINEAGSAAAFQAVGTSYATHGIPMVPVYIFYSMFGFQRTGDQFWAAGDQ
                     LTRGFIIGATAGRTTLTGEGLQHADGHSPLIAGTNRAVVTYDPAFGYEIRHIIKDGID
                     RMYGPEDGRDKNVMYYLTVYNEPIGQPAEPEDVDVEGIIKGIHKFADAPAGEGPQAQI
                     LASGVAVPWALEAQELLQAHWNVHASVWSVTSWSELRREALDCEQEAFLHPEREPRTP
                     YLTAKLADAAGPFVATTDFDHLVPDQVRAWIPGDYATLGADGYGFSDTRAAARRYFKI
                     DGPSTAVRVLQQLERRGEVERGTTARAIERYQLHDVTKGTSGNAGGDS"
BASE COUNT          793 a         1357 c         1213 g          659 t
ORIGIN      
        1 cctaccggac tcgaaccgat gacacctgcg gtgtaagcgc agtgctctaa ccaactgagc
       61 taagggcccg cgcaccattg ttccctatcg acggaacgcc ggttaagcgg tggtgcgcag
      121 acagactaga cgagtccgag caccttgcgg taggcgtcga ggtcgcgagc gtctcccgtg
      181 gggttctcga gcgcccaggt gaccaaaccg tcggcgccaa tgtggagcgt gccgcgcttg
      241 gagcgaccca actgctcgtc gaacatgccg aacgcgcggc tgacctcgcc gtgcggccag
      301 aagtcgctga gcagggtgcc ggggaacttg ttctgatacg cccactcttg gttgacgaac
      361 ttcgagtcgc agttcaccac gaataccttc gcgtctgacg cgaccaggtc ctctgcatcg
      421 cgcaactgct caagttcata cgtgcacacg ggcgagaagg cccacggcac gaacacgagt
      481 agcacgtcgg tgccgcgcag tgccgacaac gagacctcaa ttccgtcttg gtcggaaaga
      541 gtgaagtctg gagcggcgtg acccaccagg gggtgggtca cttcgagcgg cccttctccc
      601 ccaattggat ggcagtccac gtttcgtcga ccagaaagtt cgacgtcgcg tgcaacccgg
      661 caagcgaaga tgcttcttcg acatcacggg gtaagacatg tcccggacgg cgagccttgg
      721 gagtgaggag cactaccaag gcactgccgt caatcaagga ctgcacgtcc atcagcaagt
      781 cagcgagatc gtcgtctccg tcgcggaacc acacaatcgc cgcatcggtg acatcaccgt
      841 agtcttcgtc caccaggtct tcacctgtaa cggacatgat cgctctacga agcgcgtcgc
      901 acacatcggc atcgtgcccg aattcctgta caaccatttc ctgggtgaaa cccagtcgcg
      961 acaccattgc agtgtcgagc gcttccgagc cctctgtcgt ggccacggcc cgcctgcccc
     1021 tttcgtcagt gagcacgttg gtacgtcctc gtttggacca cagtacgggt cggagtgcac
     1081 cagttgcaac ggcggtgcct gacacccgac tcgtggtacg cctcgcgcgc gcgccgccaa
     1141 agaagcggcc tggcgtgaca ttggaccggg atgtggcaag aatgttctgg tacgcggtaa
     1201 ccccgcaaac cacctgcacg tgacatgagc gcgacacctc gcgctgaaac gaaaggtccc
     1261 ctgtggcaca gcacggcgcg atcgacaaag atccagaaga gaccaacgag tggcttgagt
     1321 cgctcgacgg aatgatcgag aacggcggcg ccactcgcgc tgagtacgtc atcgatcgcc
     1381 tggtcgaacg cgcgtcgttc aaacagctct cggtgcccct tagtctcaac acgccgtacg
     1441 tcaacacgat ccacgccgac aacgagcctg tgtaccccgg aaacgaacca ttcgagcgtc
     1501 gctaccgcgg ctggatccga tggaacgcgg ccgtcatggt cacgcgcgcc caggctgccg
     1561 gcaagggcgt cggcggacac atttcgtctt atgcctccgt ggccacgctg tatgaagtgg
     1621 gactcaatca cttcttcaag ggcaaggacc accccggcgg cggcgatcac atctacttcc
     1681 agggtcacgc ggctcccggc gtgtacgcgc gcggacttgt cgagggccgc ttctccgagg
     1741 acgacctcga ctcgttccgc caggagaagt ccggggtggg ccgcggcctg tcttcgtacc
     1801 cgcacccgcg tttgatgccc gacctatggg agttccccac ggtgtccatg ggtcttggac
     1861 ccgcatcggc catctaccag gcctggaccg accgctacct gaaactgcgg gggatcaagg
     1921 acacgtcaca gcagcgcacg tgggcattcc ttggcgatgg cgagatggac gagcccgaat
     1981 cgcgcggcat gctgcagttg gctgcaaatc agaagctcga caacttgacg ttcgtggtca
     2041 acgcgaacct ccagcgcctc gacggccccg tgcgcggcaa cggcaagatc atccaggagc
     2101 tcgaggcgtt cttccgtgga gccggatgga acgtcatcaa ggtgatctgg ggccgcagct
     2161 gggacccgct actcgcgcgc gaccacgacg gagccctcac caacctcatg aacaccgtcc
     2221 ccgacggcga cttccagacc ttccgcgccg agtccggagc cttcattcgc gatcagttct
     2281 ttggcggcga cccccgcgcc aaggaacttg tcgccgacat gacggacgac gagatttggg
     2341 cgctcaagcg cggcggccac gactaccaga aactctacgc cgcgtactcc cgcgccgctc
     2401 aggagaagaa cggcaagccc accgtcatcc ttgccaagac gatcaagggc tatggcctcg
     2461 gcaccaactt cgcggcacgc aactcgactc accagatgaa gaagcttgcg gccgcggacc
     2521 tcaaggtgct gcgcgacacg ttcagcatcc cgttcaccga tgagcagatc gacgcggacc
     2581 cgtacaaccc accgtactac caccccggct acgacgaccc ggccatccag tacatgttgg
     2641 atcgccgcaa ggacctcggc ggattcgtgc ccgagcggcg cgactcctac gacccgatcg
     2701 agttgccaga ccccgaggtg tacgagttgc tggcgaaggg gtccggcaac catgaggtcg
     2761 ccacgaccca ggccttcgtg cgactgctca aggacctcat caaggacaag aacttcggtc
     2821 accgcatcgt gccgatcatc cccgatgagg cacgcacctt tgggctcgac gccatcttcc
     2881 cgtcggccaa gatcttcaac actcaggggc agaactactt gccggtggat cgcgagctga
     2941 tgctcagcta caaggagtca gagtccgggc agatcatgca cacgggcatc aacgaggccg
     3001 ggtctgccgc cgccttccag gccgtcggaa cgtcgtacgc gacgcacggc atcccgatgg
     3061 tgccggtcta catcttctac tccatgttcg gattccagcg caccggcgac cagttctggg
     3121 ctgcgggcga ccagctcacc cgcgggttca tcatcggcgc gaccgctgga cgcaccacgc
     3181 tcacgggtga gggacttcag cacgccgacg gccactcgcc gctgatcgcg ggcaccaacc
     3241 gcgccgtggt cacgtatgac cctgcgttcg gatacgagat ccgtcacatc atcaaggacg
     3301 gcatcgaccg catgtacggg ccagaggacg gccgcgacaa gaacgtgatg tactacctga
     3361 cggtctacaa cgagcccatc gggcagcccg ccgagccgga ggacgttgac gtcgagggca
     3421 tcatcaaggg catccacaag ttcgcggacg ctcccgccgg cgagggcccg caggctcaga
     3481 tcctcgcatc cggcgtcgcc gttccatggg cgctcgaggc ccaggaactg ctgcaagcgc
     3541 actggaacgt tcacgcatcg gtctggtccg tcacctcgtg gagcgagttg cgccgcgagg
     3601 ccctcgactg cgagcaggag gcgttcctgc acccagagcg cgaacctcgc acgccgtacc
     3661 tcacggccaa gcttgccgac gcggcgggcc cgttcgtcgc caccaccgac ttcgaccacc
     3721 tcgtgcccga ccaggtgcgc gcgtggattc cgggcgacta cgcgacgctc ggcgccgatg
     3781 gctacggctt ctccgacacc cgtgctgcgg cgcgccgcta cttcaagatc gacggaccgt
     3841 cgacggcggt gcgcgtgctc cagcaactgg agcgtcgcgg cgaggtggaa cggggcacca
     3901 cggcgcgcgc catcgaacgg tatcaattgc acgacgtcac caagggcacg tccggaaacg
     3961 ccggaggcga ctcctaaccg accgtccctt gcgcgggtaa gagagcctag gccccgggct
     4021 tg
//
LOCUS       DASRKH010000446         2033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_61067, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000446 DASRKH010000000
VERSION     DASRKH010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61067"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..216
                     /locus_tag="VFC06_07170"
                     /pseudo
     CDS             1..216
                     /locus_tag="VFC06_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018778387.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase"
     gene            complement(177..1433)
                     /locus_tag="VFC06_07175"
     CDS             complement(177..1433)
                     /locus_tag="VFC06_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HZL81695.1"
                     /translation="MSTVLSKLKSFMSWTVHGDGKRISTNQAVAPDERLTYPRTIGIG
                     AQHVLAMMGSTLLVPAITGMPASTTLFFSAIGTALFLTITGNKVPSYLGSSFAFIAPL
                     AAAGVNAGNGPLDGATIARGLGGILLAGVLLAAVGALVHLLGAGWIQKLMPSAVTGTI
                     VALIGLNLAGAAKGMWNGSPWTALVTLAAVLLLMVLSKGFLGRIAILAGAVLGGVFAA
                     FRGEWDGAGAVIGNASWFGFPEYHTPTFEGSVLIAFVPVVLVLIAENIGHVKSVAAMT
                     NRDLDPMTGRALFADGVATTLAGLGGGSGTTTYAENIGVMAATRVYSTLAYWVAAGVA
                     LLLSLVPKFGALVAVIPIGVLGGLATLLFGLIAVLGARIWVQNGVNFSHPVNLVTATT
                     SASTRAGVVGGVSWVPRFFATHDSPW"
     gene            1696..>2033
                     /locus_tag="VFC06_07180"
     CDS             1696..>2033
                     /locus_tag="VFC06_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012877184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZL81696.1"
                     /translation="MANVVRGPSMHDVAARAGVSHQTVSRVLNGFEGVRPGTRDRVVA
                     AITELGYRRNVAARSLATGRSQAVGVLIPDTPEFGPTSSFYAVERALRDAGFQALSTA
                     TGNDAESVDEA"
BASE COUNT          358 a          653 c          702 g          320 t
ORIGIN      
        1 ctcatcgcgg tgcttggcgc gcgcatctgg gtgcagaacg gcgtcaactt ctcccacccg
       61 gtgaacctcg tgaccgcagc cgtcgcgctc atcatcggca ccgccgacta cgtggcggtg
      121 tggggcaaga tgacgttcaa cggcatcgcg ctgggcacca tcgcggccct ggtgatctac
      181 cacgggctgt cgtgggtcgc gaagaagcgg ggcacccaag acacgcctcc cacgaccccc
      241 gccctcgtcg aggcggacgt agtggcggtc acgaggttca ccgggtggga gaagttgacg
      301 ccgttctgca cccagatgcg cgcgccaagc accgcgatga gaccgaagag gagggttgcg
      361 agaccgccca ggaccccgat cggaatgacg gccacgaggg caccgaactt gggcacgagg
      421 ctcaggagga gggccacccc agcggcgacc cagtaggcga gggtcgagta cacccgagta
      481 gccgccatca cgccgatgtt ctctgcgtac gtggtggtac cggagccgcc gccgaggccg
      541 gccagggtgg tcgccacgcc gtcggcgaag agcgcccgac cagtcatggg atccaggtcc
      601 ctattggtca tcgccgcgac cgacttcacg tgtccgatgt tctcggcaat gagcacgagc
      661 accacgggca cgaacgcgat caggacagat ccctcgaacg tgggcgtgtg gtactcgggg
      721 aacccaaacc acgaggcgtt gccgatgacg gctcccgccc cgtcccactc gccgcggaag
      781 gcggcgaaca cgccgccgag gacggcgccc gcaaggatcg cgatgcgccc caggaagccc
      841 ttggagagca ccatgagcag cagcacggcg gccaacgtca cgagagcagt ccaggggctg
      901 ccgttccaca tgcccttggc tgccccggcg aggttgagtc cgatgagggc cacgatggtt
      961 ccggtgaccg cggagggcat gagcttctga atccagccag cgccaagcag gtgaaccaag
     1021 gcgccgactg cggcgagcaa cacaccggcg agcaggatgc caccgaggcc cctggcgatg
     1081 gtggcgccgt cgagcgggcc gttgcctgcg ttgacgcccg ccgccgcgag cggagcgatg
     1141 aacgcgaagc tcgagccaag gtaggacggc accttgttgc ccgtaatcgt caggaacagc
     1201 gctgtaccga tcgcgctgaa gaacagggtg gtcgaggctg gcataccggt gattgctggc
     1261 accaacaggg tcgaacccat cattgccagg acgtgttggg cgccgatgcc gatggtgcgc
     1321 gggtaggtca ggcgctcgtc gggcgccacg gcctggtttg tcgagatgcg cttgccgtcg
     1381 ccgtggacgg tccagctcat gaagctcttg agttttgaca ggacggtaga catggggctc
     1441 ctttgccttg ttttctccat cgtcgggcct gccactgctc ccgacctggt cgaactctag
     1501 gggtaggagg tcccgggttc ttaagcccca ggtcccgcgc gttgctagcg gcgaagaata
     1561 ggtctcgcgg ggccgcccgg tgtgtgcgat gatgttaacg taaacattcg gcgccatcga
     1621 gggcgcgcct cgtcgtctcc aaggcggcat ggcggctagt ctttcgatgg cggtactcaa
     1681 gacagcgagg tttcaatggc gaacgtggtg cgcggtccga gcatgcatga cgtcgcagcg
     1741 cgcgcgggag tctcgcacca gaccgtgtcg cgagttctca acggtttcga gggcgttcgt
     1801 cccggcacca gggaccgagt ggtcgccgcg atcacggagt tggggtaccg ccgcaacgtc
     1861 gccgcgcggt cacttgccac cggtaggtcg caggccgtcg gcgtgctgat cccggatacg
     1921 cccgaatttg gccccacctc cagtttctat gcggtcgagc gcgccctcag ggacgccggc
     1981 ttccaggccc tgagcacggc gaccggcaat gacgccgagt cggtcgacga ggc
//
LOCUS       DASRKH010000447         2039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_41082, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000447 DASRKH010000000
VERSION     DASRKH010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41082"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="VFC06_07185"
     CDS             complement(<1..886)
                     /locus_tag="VFC06_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZL81697.1"
                     /translation="MDDLSTGEGLLAEALDSLGVRSEFTEGGDTGADLTITTDTGTYP
                     VKVKRWASFPDSATERNPDLRIAPDPRWVRVVVADRVSASARARLTELGWAWLDLRGH
                     LRLRAPGLVIDSDVPPLRSRPERVDAIGGRAGLEVACAILEDPRSGHTVRELARTTKR
                     APSTVSEVVKAMRLEGLISAEGFDADAELFWRVAGRWNLRRTYLARLPQAGRSPDALE
                     LGFGGDVTDGPGWAMTGDHAAAADGAPVGVRADARVDFYVPSRAAARRAVAILGQASD
                     RAAAACAVIVPPVPRACVN"
     gene            1077..1298
                     /locus_tag="VFC06_07190"
     CDS             1077..1298
                     /locus_tag="VFC06_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZL81698.1"
                     /translation="MAHGRLRSPADVGRAVRHARGELGMSQVELAERLGVSQRWLSEL
                     ETGKPKILDARLFDVLAQVGLNLAWSLDD"
     gene            1291..>2039
                     /locus_tag="VFC06_07195"
     CDS             1291..>2039
                     /locus_tag="VFC06_07195"
                     /inference="COORDINATES: protein motif:HMM:NF019421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HZL81699.1"
                     /translation="MTDVSLDVELHGHHLGRVERRPEGARWMAGPDALERYGIGSTVL
                     SLALPLEATPAPVAATEAFFGGLLPEGARLDALLSDNNQLTRGNLVGLLAAVGRDVAG
                     GLVLPGPAVTTLGPLLSDAEAAHEVANPRGYLAGGGSAMAGIRPKVALARSTAGWHAA
                     RDGHPSSHLVKPGSPGSLRDARAEAWVMGLAHRVGLTYSDVSLEWFGELPAVVIERFD
                     RVATPDGGLRRLHQEDAAQALGLAWGGDAKF"
BASE COUNT          311 a          691 c          710 g          327 t
ORIGIN      
        1 ggttcacgca ggccctcggc accggaggga caatgaccgc acaggcggct gcagcgcggt
       61 cgctggcctg accgagaatc gccacagccc gcctcgccgc ggcgcgcgac ggcacgtaga
      121 agtcgacacg ggcatctgct cgaactccca ccggcgcccc gtcggccgcg gccgcatggt
      181 cgccggtcat ggcccaaccc gggccgtccg taacatcgcc gccgaatccg agctcgagtg
      241 cgtcggggga acggcccgcc tgcgggagtc gtgcgagata ggttcgcctg aggttccacc
      301 gtcccgcgac tcgccaaaag agctcggcgt ccgcgtcgaa accctcggcg ctaatcaaac
      361 cctccaagcg catggccttc accacctcgg acaccgtgct gggcgcgcgc ttggtggttc
      421 gtgcaagttc gcgcaccgtg tgacccgacc gtggatcctc gaggatcgca caggcgactt
      481 cgaggccggc gcgacccccg atcgcatcga cgcgttccgg gcgcgatcgc agtggcggca
      541 cgtcgctgtc gatgacgagc cccggtgcac gcagcctgag gtgcccgcgg aggtcgagcc
      601 aagcccaccc gagttctgtg agccgggccc gtgcggatgc agacactctg tccgcaacca
      661 cgactctgac ccaccggggg tccggcgcta tgcggaggtc cgggtttcgc tcggtcgccg
      721 agtcggggaa actcgcccac cgcttgacct tcacggggta cgtcccggta tcggtggtga
      781 tggtgagatc cgcgcccgtg tcaccgccct cggtgaactc ggaacgcact ccgagtgagt
      841 ccagcgcctc cgcgagcaac ccttcacccg tgctgagatc gtccatgacg cagtggtacg
      901 caggtgttcg gtgttcggtc aataccgaac accgaacacc gaaggccaag gttccggagg
      961 tcttgccttc cgctatccct ttcgtgttat agccttcccg ctattacgtc agaggaatac
     1021 ggtatactca actacgttca ggtaatagtg caaatcacct gcgggaggac ggcatcatgg
     1081 ctcacggcag gctccggtcc ccggccgacg tgggacgcgc cgtgcggcat gctcgtggcg
     1141 agctcgggat gtcccaggtg gagttggccg agaggcttgg cgtctcgcag cggtggctgt
     1201 ccgaactgga gacgggcaag cccaagattc tcgacgcccg actgttcgac gtgctcgcgc
     1261 aggtggggct gaacctcgcg tggagcctcg atgactgacg taagcctcga cgtcgaactg
     1321 cacggacatc acctgggtcg cgtggagcgt cgccccgagg gtgccagatg gatggctggc
     1381 ccggacgccc tcgagcgtta cggcatcggc tccaccgtgc tgtcgctcgc cttgccgctg
     1441 gaggccaccc cggcacccgt ggccgcgacc gaggcgttct ttggcggcct gctgcccgaa
     1501 ggggcgcgtc tagacgcgct gctgagcgac aacaaccagc tgacccgcgg caacctggta
     1561 gggctgctgg ccgcggtggg gcgcgacgtt gcgggcggac tcgtgttgcc tggccccgcg
     1621 gtcaccaccc ttggcccgct gctgtccgac gctgaggccg cgcacgaggt ggcgaaccca
     1681 cgcgggtacc tggccggcgg cggctcggcg atggcgggaa tacgacccaa ggtggcgttg
     1741 gcccggtcga cggcgggttg gcatgctgca cgcgacggcc acccgtcgtc gcacctcgtg
     1801 aagcccgggt cgcccgggtc gctgcgcgat gcgcgcgccg aggcatgggt catggggctg
     1861 gcgcaccggg tgggactgac ctactcggat gtgagcctcg aatggttcgg cgagctgccc
     1921 gccgtggtga tcgaacgctt cgaccgtgtg gcgacgcccg acgggggcct gcgacggctc
     1981 caccaggagg atgcggccca ggcgctcggc ctggcctggg gtggcgatgc gaagttcga
//
LOCUS       DASRKH010000448         3880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_2818, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000448 DASRKH010000000
VERSION     DASRKH010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2818"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VFC06_07200"
     CDS             <1..232
                     /locus_tag="VFC06_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81700.1"
                     /translation="RVVFNSAQTAARCAEWRHHTMLPVVSTQGRADPGEQSTAVHTRS
                     VDGAAQTAGGIAKGRRFSAKRAVVEDLRRSST"
     gene            complement(419..2938)
                     /locus_tag="VFC06_07205"
     CDS             complement(419..2938)
                     /locus_tag="VFC06_07205"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZL81701.1"
                     /translation="MAPIIRSLSVKGYRAYGAAEQTLNLLADIAVVWGPNSTGKTSLA
                     EAFEFLLTGRITRRELMASTQDEFADALRNAHLAAGEEVHVAARITAADGTAHEIRRV
                     LTGDYAKRRDCTSRLEIDDAAAAEGDLARFGIAVSQPPLGAPILAQHTISHIFSVGPQ
                     DRATYFKTLLEVTDLDDLRNDIAALAGELTPPHDPLLTKFDTCAAVPALKPALAGMLR
                     TNTDLAALTVGIEDAARALIEAVGETVPETLDGRLAAIDKILADRRSKTFPLCGFERK
                     ELAGWNPPPPDIWTRLDSYLDEREKVDEETRHLVALLDEALKLPAIFGITEPVDCPLC
                     GTESALTPERVELIRQHVEDSKDFKTAETAAKSALSQLSASAEALATGAEAALPRYLK
                     ATAAKRRETGFTVARIRGLLGDRAAELANPWLARVRPLARAGIALRRGALAAGALVEE
                     EIAGIAATLDPDKLSAAFSAVAMLRATYAVAIDAYEALAAPLGTALNEVLDAQSDTSG
                     WQDFLDIARQPVMLRAALIERAARVAVGKELDAALSQVDRGKEQVLEDKFSDYSTLIQ
                     AWWERLRPDEPTFFSAVQPRKGAKRTIDFKAGLSANPDRSAPKVRDVIAVFSQSQLHC
                     LGLAMFLARAQHEGLGFIILDDPVLSSDEDYRVHFNSTVLTELLKLPMQVVVLTQDHN
                     TWKELEIRYRHLGVSTAQLFIETPAEGGIIENTSDALLAKINRAKSLARGGHPDSRKE
                     CGIQLRDAGERFCKEMLAEDRHGKGEAAASLTDYDGKMLEWLCPRVDPLLDHDASHPG
                     KLTVFKNTVNNACHDNAPPGNAEMTHAHGEIRFFVKDYLGR"
     gene            complement(3670..>3880)
                     /locus_tag="VFC06_07210"
     CDS             complement(3670..>3880)
                     /locus_tag="VFC06_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81702.1"
                     /translation="RNADTPEMVERIVDDIDEIVRYVRFDGCQNTHAGEREVKMALRK
                     TLFKYKLHQDQELFGRAYEYVRMYY"
BASE COUNT          695 a         1152 c         1280 g          753 t
ORIGIN      
        1 ccgcgtggtc ttcaactcag cccagacagc agcgcgatgc gctgagtggc gccatcatac
       61 aatgctgccc gtggtttcga cgcagggacg cgctgacccc ggtgagcaat cgaccgccgt
      121 gcacacacga tctgttgatg gtgcagccca gaccgcgggg ggaatcgcca agggaaggag
      181 gttctccgca aagcgggctg tggtggagga tcttcgaaga tcatccacat gagaagccgt
      241 ccaccaaagg ggctgtggtg gaggcccttc gaacatctgt tggttcccgg tgacggtaac
      301 gcccaggatc agctgcggcg cggcgcacct tctggtgcgt gctgttgttg ggccgcttgg
      361 gacccaagac attatcggca atgctgtccg tgagagagac ctgaaactta tgcataactc
      421 atcgccctag ataatccttc acgaaaaagc ggatttcgcc gtgggcatgc gtcatctcgg
      481 cattgcccgg gggcgcattg tcgtggcagg cgttgttcac ggtgttcttg aagaccgtga
      541 gcttgccggg gtgggaagca tcgtggtcga gcaacggatc gacgcggggg cacagccatt
      601 caagcatctt gccgtcgtaa tcggtgaggg aggcagccgc ttcacctttg ccgtgccggt
      661 cctcggcgag catttctttg cagaaacgct cgccggcgtc gcgcaactgg atgccgcact
      721 ccttccgaga gtcgggatgc cctccgcgcg caagcgattt ggcacggttg atcttggcga
      781 gcagcgcgtc gcttgtgttt tcaatgatgc cgccttcggc gggggtttcg ataaagagct
      841 gggcggtcga tacgccgaga tggcgatagc ggatttcaag ttccttccat gtgttgtggt
      901 cttgggtgag gacgacgacc tgcatgggca gcttgagaag ctcggtcaac accgtcgagt
      961 tgaaatggac tcggtagtcc tcgtcgctgg acagcacggg atcgtcgaga atgatgaaac
     1021 cgaggccctc atgctgggcg cgagcgagaa acatggcgag gcccagacag tgaagctgag
     1081 actggctgaa cacggcgatc acatcgcgca ccttcggggc tgaacggtcg gggttggccg
     1141 agagtccggc cttgaagtca atggtgcgtt tggccccctt gcggggctgc acggcggaga
     1201 agaacgtcgg ctcgtcgggc cgtagccgct cccaccaggc ctgtatcagg gtgctgtaat
     1261 cggagaattt gtcctcgagc acctgctcct tgccgcggtc gacttgactg agcgcggcat
     1321 cgagttcctt tccgacggcc acgcgggcgg cgcgttcaat cagggcagcg cgaagcataa
     1381 cgggctgccg cgcgatatcc aggaagtcct gccagccgct agtgtccgac tgtgcatcga
     1441 ggacctcgtt gagagccgtg ccgagaggag ccgccagcgc ttcataggcg tcgatcgcga
     1501 cggcgtaggt agcgcgcagc atagcgaccg cggaaaaagc ggcgctcagt ttgtcggggt
     1561 cgagggtggc ggcgatccct gcgatctcct cttcgacgag cgcgccagcc gccagtgcgc
     1621 cacggcgcag ggcgatcccg gcccgggcaa ggggcctgac ccgggcaagc cacggattgg
     1681 cgagctcggc ggcgcggtcg cccaggagtc cccgaatgcg ggcaacggtg aatcctgtct
     1741 cgcggcgctt ggcggccgtc gccttgagat agcggggcag ggcggcttcg gcaccagttg
     1801 ccagcgcctc ggctgacgcg gaaagctgtg aaagagcgga tttcgcagcc gtctcggcag
     1861 tcttgaagtc cttcgagtcc tcgacgtgct ggcggatgag ctccacgcgt tccggtgtca
     1921 gggcggattc tgtgccgcac agcgggcaat ccacgggctc agtgatcccg aaaatggccg
     1981 ggagcttcag agcctcgtcg agaagcgcga caagatggcg cgtttcttca tcgactttct
     2041 cgcgttcgtc tagataggaa tcaagccggg tccagatgtc cgggggcggc ggattccaac
     2101 cggcgagttc cttgcgctcg aacccgcaca gcggaaatgt cttgctccgg cggtcggcca
     2161 gaatcttatc gatcgccgca agccgtccgt cgagcgtctc cggtaccgtt tcgccgacgg
     2221 cctcgatgag ggcccgcgcc gcgtcctcaa tcccgactgt gagcgctgcc agatctgtat
     2281 tggtgcgcaa catgccagcg agcgcgggct tcagggccgg gacggccgcg caagtgtcga
     2341 atttggtgag cagtgggtcg tgcggcggtg tcaactcgcc cgctagcgcc gcaatgtcgt
     2401 tgcgaagatc gtcgaggtcg gtgacttcga gcagggtctt aaaataggtg gcgcggtctt
     2461 gggggccgac ggagaaaata tggctgatcg tgtgctgtgc gaggatcggg gcccccaagg
     2521 gcggttgcga caccgcaatg ccgaaccttg ccagatcgcc ctcggcggcc gctgcgtcgt
     2581 cgatttcgag gcgggacgta caatcgcgtc tcttggcgta gtcgccggtg agaacgcgcc
     2641 tgatctcgtg cgccgtgccg tccgcggctg tgatgcgggc ggccacatgg acttcctcgc
     2701 cggcggcgag atgggcgttg cgcaaggcgt cggcgaactc atcctgagta ctcgccatta
     2761 actcgcgccg ggtgatgcgg ccggtcagca gaaactcgaa ggcctcggca aggctggtct
     2821 tgcccgtgct gttcggtccc cagacaacag caatgtccgc caacaggttg agggtctgct
     2881 cggcggctcc gtaggcgcga tatcccttga ctgaaagcga cctaattatc ggtgccatca
     2941 atgctccctc tcacccgttc aagtcgttca aggagaacgg ccttcatgga ttccggatgg
     3001 gctgggtcgc ggttgtgatg ggcccgcacg agtcgggcgg cgagcgaacc gtcctctagg
     3061 tgtgccaaga ggcgctcgga gttgttcttc ggttccgcct tgtctggcat ctctaggcat
     3121 ctccccagcc tcgctgtgga atacaacttc gccttcgcat gttgatctgc ggcctaacaa
     3181 gtactatgca gtctgcagaa cttcatgact gcagtcccgt ttgtctgcat gctgcccgcg
     3241 acagtcgact ggaacggcgc atccagtatt ggtcgactgc gggaatcccg aggctctcgg
     3301 cgaagtctcc ccaggagatg ttgcgactgt ataggtcccc tcaaagcagg tcaagcacct
     3361 agcccacctc cgtgcgatta gagaaaggca cccaagcgtt cgacgaactg ctggcgggag
     3421 agctccaacc cctcaccgaa taccgccgaa gcctcctgtc gcacatgcgc gcggaagcca
     3481 tccgagaggc cgtcctcccc tcgggccaca ggagatgcga atgacgattc agtcctgttg
     3541 ccacacgagg ctgggaagga ggcaagcacc acaatcgggg cgcgattacg ccctcgcggt
     3601 ggaggtcgta gcgcagccca aggcttcaca cgtgcgaatt gacactatcg cagaatggtg
     3661 tgcggccgct cagtagtaca tccgcacgta ctcataggcc cgcccgaaga gctcctggtc
     3721 ctggtgtagc ttgtacttga acagcgtctt gcgcagggcc atcttgacct cgcgctcgcc
     3781 ggcgtgggtg ttctggcagc cgtcgaagcg gacgtagcgc acgatctcgt cgatatcgtc
     3841 tacgatgcgc tccaccatct ccggcgtgtc ggcattgcgg
//
LOCUS       DASRKH010000449         2225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_34214, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000449 DASRKH010000000
VERSION     DASRKH010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34214"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..1312
                     /locus_tag="VFC06_07215"
     CDS             221..1312
                     /locus_tag="VFC06_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009884904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HZL81703.1"
                     /translation="MPRTGRPKPELTLSDEEREQLLRWSRRAKSSQALALRSRIVLGC
                     GDGLDNKTVAERMGCSTNTVSKWRARFLTDRLDGLVDEPRPGRPPTITAAQVEDIVAA
                     TLESTPKNATHWSRAKMAQRSGLSKSTVGRIWRAFELKPHREDGFKLSNDPQFVEKVY
                     DIVGLYLDPPEAAVVYCVDEKSQVQALARSQPAFPMMPGMPEKRTHDYVRHATTSLFA
                     AFNTADGTVISSIHRRHRAIEFKKFLVKIDADVPDELEVHLVCDNYSTHKSPSILTWL
                     EAHPRFHIHFTPTYSSGINQVERFFAYVTADLLQRSDHRSVQALERDIRNWVKGWNEN
                     PKPFIWTKPAEQILESLKRLLQRTTGAGH"
     gene            complement(1466..>2225)
                     /locus_tag="VFC06_07220"
     CDS             complement(1466..>2225)
                     /locus_tag="VFC06_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase UvrD2"
                     /protein_id="HZL81704.1"
                     /translation="LGAQVREAIVDAGWSEEPPAARGAVRERWDALQALVELADSFDV
                     DAVAATGEPATMRDFVEHLADRASAQHAPSVDGVTLTTIHAAKGLEWDAVFLVGLSEG
                     LLPISMAETDEAVEEERRLLYVGITRAREHLNASFARSRKEGGRSTRKRTRFLEKWWP
                     DAHATPQRRPQRTAAHELNGDEAKLFEALKAWRLELAQVTSKPAFTVLVDTSLVAIAR
                     HRPQTMGELAQVHGVGASKLDRYGADILGIVAAH"
BASE COUNT          394 a          764 c          700 g          367 t
ORIGIN      
        1 gtcctgaagt tcaacgaatg cgccgttcgc caagaaaccc tcgcgatcag cggcagcaac
       61 gagcccttct tcgactctgc tgctcgtccc gcgggtccca gcgacctgta caacacctac
      121 caattgggct catcacaatc gagggtgtag ttggggtctg aatccgccgg tctctagtgt
      181 cgtgcgccgg tatttctttg gttaagtggt agaatggggt atgccgagaa cgggacgccc
      241 gaagcctgag ttgacattga gtgatgagga gcgcgagcag ttgctgcgct ggtcccggcg
      301 ggcgaagtcc tcgcaggcat tggcgttgcg gtctcggatc gtgctgggtt gcggcgatgg
      361 cttggacaac aagacggtgg ctgagcggat gggatgctcg acgaataccg tcagcaagtg
      421 gcgcgcccgg ttcctcacgg atcgactgga tggtctggtc gacgagccgc gtcccgggcg
      481 gccgccgacg atcactgcgg cccaagtcga agacattgtg gccgcgacgt tggagtcgac
      541 gccgaagaac gccacgcact ggtcgcgggc gaagatggcc cagcgctccg ggctgtcgaa
      601 gtccacggtc ggacggatct ggcgggcgtt cgagctgaaa ccgcaccggg aggacgggtt
      661 caaactctcc aatgacccgc agttcgttga gaaggtctac gacatcgtcg ggctgtacct
      721 ggacccgccc gaagccgccg ttgtctactg tgtggacgag aagtcgcagg tgcaagccct
      781 ggcccggtcg cagccggcgt ttccgatgat gccgggtatg cccgagaaac gcacccatga
      841 ctatgtccgc cacgctacga ccagcttgtt cgcggcgttc aataccgccg atggcaccgt
      901 catctccagc atccaccgcc ggcaccgggc gatcgagttc aagaagttcc tggtcaagat
      961 cgacgccgac gtccccgacg aactcgaggt ccacctggtc tgcgacaact acagcaccca
     1021 caagagcccc agcatcctca catggctcga agcacatccc cggttccaca tccacttcac
     1081 cccgacctac tcgtcgggga tcaaccaggt cgagcgcttc ttcgcctacg tcaccgccga
     1141 cctgctgcaa cgctcagacc accgcagcgt ccaagcactc gaacgagaca tccgcaactg
     1201 ggtcaaagga tggaacgaga acccgaagcc cttcatctgg accaagcccg cggaacagat
     1261 cctcgaatcc ctcaaacgac ttctacagcg aactaccggc gcaggacact agcgcctccg
     1321 ccgcgcctcc acatcccgac tgcggatgcg gtgcggcagg acccatggga gacgagctcg
     1381 cggcccgcag ggctcgcatg cccggcacac cgtgacgctc cgcgatcccg caggctcgct
     1441 cccgcaggct cgctcccgca gactctcagt gcgccgcgac gatgcccagg atgtccgcgc
     1501 cgtagcggtc cagcttggac gccccgaccc cgtgcacttg tgccagttcg cccatcgtct
     1561 gcgggcggtg tcgcgcgatt gcgacgagag acgtgtccac gagcacggtg aaggctggct
     1621 tggacgtgac ctgcgccagc tcgagccgcc aggccttgag cgcctcgaat agcttggcct
     1681 cgtcgccatt gagttcgtgg gccgcggtgc gttgcgggcg tcgctgaggg gtcgcgtggg
     1741 cgtcgggcca ccacttctcc aggaagcgcg tgcgcttgcg ggtcgagcgc ccaccctcct
     1801 tgcgcgaccg cgcgaagctg gcattcaggt gctcgcgggc gcgagtgatg cccacgtaga
     1861 gcagccgacg ctcctcctcg accgcctcat ccgtttcggc catggagatg ggcagcaggc
     1921 cctcggagag ccccaccagg aacacggcgt cccactccag gcccttggcg gcgtggatcg
     1981 tggtgagcgt caccccgtcc accgaggggg cgtgctgggc ggaggcgcga tccgccaggt
     2041 gctccacaaa gtcccgcatc gtggccggct cgcccgtggc ggccaccgcg tccacatcga
     2101 aactgtcggc gagttccacg agcgcctgga gtgcgtccca acgctcgcgc accgcgccgc
     2161 gagccgcagg cggctcctcc gaccatccgg cgtcgacgat cgcctcgcgc acctgcgcac
     2221 ccaac
//
LOCUS       DASRKH010000450         2272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_30272, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000450 DASRKH010000000
VERSION     DASRKH010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30272"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..1727
                     /locus_tag="VFC06_07225"
     CDS             162..1727
                     /locus_tag="VFC06_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZL81705.1"
                     /translation="MPHDPASPSSVPGGIDPEDLATTLRVLASLNEVGEDHPDFVAVR
                     HATSRMVKAVKQTRRHDKRAAITKADGDVVAATATGAPDRIDDETRGLTLTATASARI
                     AGTFTKPRPCYICKQKYNVVDAFYHQLCPECAALSHAKRHARTDLTGKRALLTGGRAK
                     IGMHIALRLLRDGAHTTITTRFPHDAVRRFAAMDDAAQWLHRLTVVGIDLRDPSQVVS
                     LAESVAANGPLDILINNAAQTVRRSPGAYAPLADAESVPVPPGLWPEGRGPRMVTLGR
                     TADQHPHALQDSVVAHPLLASAHTAEMGVPARSDGVPDVARINAERLTALALAPGSSS
                     LAKLADGSAIDAGGLIPDLHDTNSWVHGVGGVDALELLEVQLCNSTAPFILINQLRPQ
                     LAAAAASTASKSGRAYVVNVSAMEGVFSRGYKGPGHPHTNMAKAAVNMLTRTSAREMF
                     ETDSILMTSVDTGWITDELPHPTKVRLAEEGFHAPLDLVDGAARVYDPIVRGEAGEDL
                     FGVFLKDYAPSGW"
     gene            1780..>2272
                     /locus_tag="VFC06_07230"
     CDS             1780..>2272
                     /locus_tag="VFC06_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012622966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZL81706.1"
                     /translation="MSVDVGSSSTTPLVRVEHFGMSFGRTEVIQDLSFEIHRGETFGF
                     LGSNGSGKTTTIRALLGIYRPTRGVLHIDGREFSPDNGDRLGYLPEERGLYQKESVVD
                     VMTYFGRLKGLDRATAKQWSLDYLERVGLADRASVRVDKLSQGQQQKVQLGVTIMNDP
                     ELLI"
BASE COUNT          408 a          809 c          691 g          364 t
ORIGIN      
        1 gcccgttgaa agtccaactc atcagatgat ctcctgccag tggcggctcg gatagccgcg
       61 ccaacacgat gctctcgacg aagatcacta gaacacaccg aaatcgctca gagttcgctc
      121 agagaacact tggggcgcct cccttgggta ggctccttcg cgtgccacac gatcccgcct
      181 caccctccag cgtgcctggc ggcatcgacc ccgaagacct ggcgacgacg ttgcgcgtgc
      241 tggcgagtct caacgaggtc ggcgaggacc accccgactt cgtcgctgtc cggcacgcca
      301 cctcgcgcat ggtcaaggcc gtgaagcaga cccgtcggca cgacaagcgc gccgctatta
      361 ccaaggcaga cggcgacgtg gtggctgcca ccgcaaccgg cgcaccagac aggatcgatg
      421 acgagacccg cggcctcacc ctgaccgcca cggcctccgc gcggatcgcg gggaccttca
      481 ccaagccacg tccgtgctac atctgcaagc agaagtacaa cgtcgtggac gcgttctatc
      541 accagctttg ccccgagtgc gcggcgctga gccacgcgaa gcgacacgca cgcaccgatc
      601 tgaccggcaa gcgcgccctg ctcacgggcg gccgcgccaa gatcggcatg cacatcgcgc
      661 tgcgtctgct gcgggacggc gcccacacca cgatcaccac tcgcttcccg cacgacgccg
      721 tccggcgttt cgcggccatg gacgatgctg cgcagtggct gcaccggctc accgtggtgg
      781 ggatcgactt gcgcgaccct tcgcaagtgg tctcgctggc cgagtccgtc gcggccaatg
      841 gtccgctcga catcctcatc aacaacgccg cgcaaacggt gcgccgctcc cccggcgcct
      901 acgccccgct cgccgacgcc gagtcggtgc ccgttccgcc ggggctgtgg ccagagggcc
      961 gcggcccgcg catggtgacg ctggggcgca cagctgacca gcatccgcac gccctccagg
     1021 actcggtcgt agcccatccc ctgctcgcgt ccgcgcacac cgcggaaatg ggcgttccag
     1081 cccgcagcga tggagttccg gacgtcgccc gcatcaacgc ggagcgactc acggccttgg
     1141 cgctcgcgcc agggtcttcc tcccttgcca agcttgctga cggcagcgcg attgatgccg
     1201 gagggctcat ccctgacctg catgacacca actcttgggt ccatggcgtg ggcggcgtcg
     1261 acgcactcga gctgctcgag gtgcagctct gcaactccac cgcgccgttc atcctcatca
     1321 accagttgcg gccccagctg gccgctgcgg cggcatcgac ggcgagcaag agcggccgcg
     1381 cctacgtggt caacgtctcc gcgatggagg gtgtgttcag ccgcggctac aagggaccag
     1441 ggcatcccca caccaacatg gccaaggctg ccgtcaacat gctcacccgc acgagcgcgc
     1501 gcgagatgtt cgagacggac agcatcctca tgacgagcgt ggataccggc tggatcacgg
     1561 acgagctccc gcaccccacc aaggtgcgac tcgccgagga gggctttcac gctcccctcg
     1621 acctggtgga cggcgcagcg cgcgtctacg accccatcgt gcggggcgaa gctggcgagg
     1681 acctcttcgg agtcttcttg aaggactacg cgccgtcggg ctggtaattc caccgcacca
     1741 cggcagctcc ctcgctacac cccggatagc atggaattca tgagcgttga cgtcggttcg
     1801 agctccacca caccgctcgt gcgagtcgag cacttcggaa tgagtttcgg ccgcacggag
     1861 gtcatccagg atctgtcctt tgagatccac cgtggggaga ccttcggctt cctcggcagc
     1921 aacggctcgg gcaagaccac gaccatcagg gccctccttg gcatctatcg gcccactcgg
     1981 ggcgtgcttc acatcgacgg acgcgagttc agcccagaca acggcgaccg actcggctat
     2041 ctcccggaag agcgcgggct ctatcagaag gagtccgttg tcgacgtcat gacgtacttc
     2101 ggcaggctga agggcctcga tcgtgcgacc gccaagcaat ggtcgctcga ctatctcgag
     2161 cgggttggct tggcggacag ggcttcggtt cgcgtcgaca aactgtcgca gggtcagcaa
     2221 cagaaggtcc agttgggagt caccatcatg aacgaccccg aactcctcat tc
//
LOCUS       DASRKH010000451         2674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_2839, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000451 DASRKH010000000
VERSION     DASRKH010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2839"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..232
                     /locus_tag="VFC06_07235"
     CDS             8..232
                     /locus_tag="VFC06_07235"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZL81707.1"
                     /translation="MAHLRQGSARVRVGDRVRQGDVVAACGNSGNSTQPHVHLQATDG
                     TDWNTARGLPIAFATPQGPALPRESEIITA"
     gene            350..556
                     /locus_tag="VFC06_07240"
     CDS             350..556
                     /locus_tag="VFC06_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin MazE family protein"
                     /protein_id="HZL81708.1"
                     /translation="MAVRERVGEYRHRMRARGYRPVQVWVPDVRTEQFAREARRQAAL
                     IARVDRRGDDQDFIEAVSVDWDEE"
     gene            553..879
                     /locus_tag="VFC06_07245"
     CDS             553..879
                     /locus_tag="VFC06_07245"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HZL81709.1"
                     /translation="MRRGELWTVAGGVYASKPRPAVIVQDDVFDLTESVTVVPLTTTL
                     TDAPLLRLRVSAGGLAGLVQDSDVMVDKLTTVRRSNLQVRIGRLSSQQMVELERLLMT
                     FLGMAR"
     gene            complement(1004..2239)
                     /locus_tag="VFC06_07250"
     CDS             complement(1004..2239)
                     /locus_tag="VFC06_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81710.1"
                     /translation="MTTVPATASIREQVLQALDPDGRMQRAIEVARHLPADARSEELR
                     RVAAEYEENITVVERYWEPLTERGWGIANVGLQVIRRAGALLDANDASASDKAIALWF
                     DAEWIARIIARVGILQDGTPDGREAFEQRQRLLKQAAHHHLAGDYAASILIVLSQIEG
                     ITAQVTAPPEGGPGRLFFSKRANRKAYVVDPTDLASIQASLDRLRDLYSDDVTQVQAD
                     GSWGRHGIAHGQEVAFDTEVNSSKYWSLLDVVVQWAMPRAHERSLKIDALRASPHTGS
                     QDVDERGRRLDDREFKVTRNALYEVCSHQSAWARRAKHFDARATDGLITNDAMEKRGL
                     PPPHGVTIHTADDGSAFWAERQTVSGWWLARGLTLNELGLVDVWYYSGQDGPSSGPSE
                     DPEQWGGQPNFAPPDWQGA"
BASE COUNT          428 a          823 c          897 g          526 t
ORIGIN      
        1 cagcctcttg gcgcatctgc ggcaggggtc tgctcgagtg cgcgtgggcg accgcgttcg
       61 tcagggcgac gtcgtcgctg cgtgcggcaa ctcgggcaac agtacgcagc cgcacgtgca
      121 cctccaggcg acggacggca cagattggaa caccgcacgg ggtttgccga tcgcgttcgc
      181 gacgcctcag ggccctgcac tcccgagaga gtcggagatc atcacggcat gatggtcgcg
      241 gccccccaac gcactcttct gccgcggggt gtgcgttggc gtcgtggggt gtcggtcttg
      301 gctccctggg gctctggctc agcagcctcg ttacatgtaa cctgttggta tggctgtcag
      361 ggagcgtgtg ggcgagtatc gccaccggat gcgggcgcgt ggctatcggc ccgtccaggt
      421 gtgggtgccg gacgtgcgga ccgagcagtt cgcgcgtgag gcacgtcgcc aggctgcgct
      481 catcgcacgg gtcgaccgcc gtggtgatga ccaagacttc atcgaggccg tgtcggtcga
      541 ttgggatgag gagtgaggcg cggcgagttg tggacggtgg ccggcggggt ctatgcgtcc
      601 aagccgcgac ctgccgtcat cgtccaggac gatgtgttcg acctgaccga gtccgtcacg
      661 gtcgtgccgc tcacgacgac gttgaccgat gcgcctctgc tgcggctgcg ggtgtctgct
      721 ggggggctgg ccgggttggt tcaagacagt gatgtgatgg tggacaagtt gaccactgtg
      781 aggcgcagca accttcaggt ccggattggg cggctgtcga gccagcagat ggtcgagttg
      841 gagcgactct tgatgacctt cctcgggatg gcccggtagc gaacaacaac gcccgttccg
      901 aacacccgga tttgccgcgg cgagggcggc cgattccgct taatgaaggt ccttgtcgtc
      961 gggtcagcgc gagggcttcc caccgagagc gtgtgttgtc gagctaggcg ccttgccagt
     1021 ctggtggggc aaagttcggt tgtccgcccc actgttcggg atcctccgaa ggtccgctgc
     1081 tgggaccgtc ttgacccgag tagtaccaga cgtcgaccag ccccagctcg ttgagagtta
     1141 ggcccctcgc gagccaccat ccggacacag tttgccgctc ggcccagaaa gccgagccat
     1201 catcagccgt atgtatcgtc actccgtgag gcgggggcag gccacgcttc tccattgcgt
     1261 cgtttgtgat cagcccgtcg gtggcccggg catcgaagtg cttagctcgt cgagcccaag
     1321 cagactggtg tgagcacact tcgtacagcg catttcgggt gactttgaac tcgcggtcgt
     1381 cgagtcgccg accacgctca tcaacgtctt ggctgcctgt gtggggtgac gcgcgtagtg
     1441 cgtcgatctt cagtgatcgt tcgtgagcgc gtggcatggc ccattgcaca actacgtcaa
     1501 gcagtgacca gtacttcgaa ctgttcactt cggtgtcgaa cgccacctcc tgcccgtgtg
     1561 cgatgccgtg ccgtccccac gatccatccg cctggacttg tgtgacgtcg tcactgtaca
     1621 agtctcgaag gcgatccaga gatgcctgga tcgaggccaa gtcagtggga tctacaacgt
     1681 acgccttgcg attcgcgcgc ttggaaaaga agagacgtcc tggacctccc tctggcggcg
     1741 cggttacttg ggcagtgatg ccttcgatct gcgagaggac aatcaggatg ctcgcggcgt
     1801 agtcgccggc cagatggtga tgggcagcct gcttgaggag gcgctggcgc tgctcgaatg
     1861 cctcccgccc atccggggtg ccgtcttgga ggatgccgac ccgcgcaatg atccgagcaa
     1921 tccactcggc gtcgaaccac agcgcgatcg ctttgtcgga cgcgctggca tcattggcgt
     1981 cgaggagtgc tccggccctc cggatcacct gtagtcccac gttggcgata ccccagcctc
     2041 gttcagtgag gggctcccag taccgctcaa cgacggtgat gttctcctcg tactcggcgg
     2101 cgacgcgccg caactcctcg gacctcgcgt ctgcaggaag gtgtcgggcg acctcgatag
     2161 ctcgctgcat ccggccgtca ggatcgagag cctgcaacac ttgctcccgg atcgacgcgg
     2221 tcgccggcac ggtggtcatg ggtggacctc tctcgctaga tggtcgccca agtcaatctt
     2281 cgcagcgggc gctgacagtc ggcggacttg tgggtgcgcg gcggccgcct gttgtcatgg
     2341 tccttcgaac tcacgaacta gcctcagaac gccctgtact gccgcacacc gcgcggtcga
     2401 taggagtcgg cgcattcgac cgctgcggac tacgtcgtaa cgccgtggcc cttgggtgtc
     2461 gcgctggggt tatgagtgac ttccgcagca gaaccgtggt gtttgggtgt accccaacgc
     2521 gacaggtcgt cgcacttggt cacctccgcg tgaggggctc ccggaccggg gcaaacccga
     2581 acagggacat cgcattccag gaggccaagg tcagggccgg taccaggtcg aggtaagccg
     2641 ggtaggtgtc cgacaggccc gccccccgca tcgc
//
LOCUS       DASRKH010000452         2036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_33496, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000452 DASRKH010000000
VERSION     DASRKH010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33496"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..1340
                     /locus_tag="VFC06_07255"
     CDS             33..1340
                     /locus_tag="VFC06_07255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZL81711.1"
                     /translation="MLSVHTSPLDQPGTGDAGGMNVYVSELSQALARRGARVEIFTRA
                     TSSAQPETAVLEGVDAHGTPLTHEAARAVLLAEGVEPGVVPPVLVHHVPAGPFEGLDK
                     NDLPGQLCAMTAGVLRSEAARRPGWYDVVHSHYWLSGQVGWLAADRWEVPLVHTMHTM
                     ARVKNAALAPGDTAEPNGRIIGEEQVVAEADALVASTAEEAHDLVELYNADPALVHIV
                     APGVDLDVFTPGGPHARAEARRALGLPLDRDIVLFAGRIQPLKGPDVLVRALGVLAAT
                     GRPVPLLVVLGGPSGRPTAVRELSALAATTGVGGDVVLRPPVPRTELARWYRAADVVA
                     MPSRSESFGLVAIEAQASGTPVLAAAVGGLRSVVTDGVSGTLVPGHDPAVWADAIADH
                     LADSGARARLAVGARRVAERYGWDAAAEQVLKVYALADAHRRA"
     gene            1405..>2036
                     /locus_tag="VFC06_07260"
     CDS             1405..>2036
                     /locus_tag="VFC06_07260"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglyceromutase"
                     /protein_id="HZL81712.1"
                     /translation="MTYTLVLLRHGESDWNAKNLFTGWVDVALSEKGVAEALRGGSLL
                     TDAGVLPDVVHTSLLRRAITTANLALDVADRHWIPVKRSWRLNERHYGALQGKDKKQI
                     RDEYGEEQFMLWRRSYDVPPPLVDLGSEFSQDADPRYADAPVVRAECLKDVLERALPY
                     WHSDIVPDLQAGKSVLVAAHGNSIRAIVKYLDDIDDATIAGINIPTGIPLR"
BASE COUNT          268 a          753 c          740 g          275 t
ORIGIN      
        1 ccttcgtgag cctcgagcag ccgcgcgtcg cgatgctgtc ggtgcacacg tcgcccctcg
       61 accagccggg cacgggcgac gcgggcggca tgaacgtgta cgtctccgag ctctcgcagg
      121 ccctggcccg gcgcggcgcc cgggtcgaga tcttcacgcg tgcgacgtcg tcggcccagc
      181 ccgagacggc ggtgctcgag ggagtcgacg cgcacggcac gcctctgacg cacgaggctg
      241 ctcgggcggt gctgctcgcc gagggcgtcg agcccggcgt cgtgccgccc gtgctcgtcc
      301 accacgtgcc cgcggggccg ttcgagggcc tcgacaagaa cgacctgccc ggccagctgt
      361 gcgcgatgac tgcgggcgtg ctgcggtcgg aggcggcgcg caggcccggc tggtacgacg
      421 tcgtccactc ccactactgg ttgtcgggcc aggtcggctg gctcgccgcc gaccggtggg
      481 aggtcccgct cgtgcacacc atgcacacca tggcgcgcgt gaagaatgcg gcgctcgcgc
      541 cgggggacac cgccgagccc aacggcagga tcatcggcga ggagcaggtc gtcgccgagg
      601 ccgatgcgct cgtggcgagc acggccgagg aggcgcacga cctcgtcgag ctgtacaacg
      661 ccgacccggc gctcgtgcac atcgtggcgc cgggcgtcga cctggacgtg ttcacccccg
      721 gtggacccca tgcacgcgcg gaggcgcgca gggccctcgg gcttccgctg gaccgggaca
      781 tcgtgctctt cgccggccgc atccagccgc tcaagggccc cgacgtgctc gtgcgcgccc
      841 tgggtgtgct cgccgcgacg ggtcgaccgg tgccgctgct cgtcgtcctc ggcgggccga
      901 gcggccggcc gacggcggtc cgtgagctgt ctgccctcgc ggcgacgacc ggtgtcggcg
      961 gcgacgtggt gctgcgtccg cccgtgcccc gcaccgagct cgcgcggtgg taccgagcgg
     1021 ccgacgtcgt ggcgatgccg tcgcggagcg agtcgttcgg cctcgtcgcc atcgaggcgc
     1081 aggcgagcgg gacgccggtg ctcgcggccg ctgtcggcgg gctgcgctcg gtcgtgaccg
     1141 acggcgtctc gggaacgctc gtccccggtc acgacccggc ggtgtgggcg gatgcgatcg
     1201 ccgaccacct ggccgactcg ggcgcgcgcg cacggctggc ggtcggcgcc cgacgcgtcg
     1261 ccgagcggta cggctgggac gccgccgcgg agcaggtgct caaggtctac gcgctcgccg
     1321 acgcgcaccg acgcgcctga cgaggaccgt tcacgggccg aacgtccccg tccacccccc
     1381 tgccgagtgg ggcaggatga gcccatgacc tacaccctcg tgctgctccg ccacggcgag
     1441 agcgactgga atgccaagaa cctgttcacc ggctgggtgg acgtcgcgct gtccgagaag
     1501 ggcgtcgccg aggccctgcg cggcggctcg ctgctcacgg acgcgggcgt gctccccgac
     1561 gtcgtgcaca cgtccctcct gcgccgcgcc atcacgaccg cgaacctggc cctcgacgtg
     1621 gccgaccggc actggatccc cgtcaagcgc agctggcgcc tgaacgagcg tcactacggc
     1681 gcgctgcagg gcaaggacaa gaagcagatc cgcgacgagt acggcgagga gcagttcatg
     1741 ctctggcgtc gctcgtacga cgtgccgccg cccctcgtgg acctggggtc ggagttctcg
     1801 caggacgctg acccgcgcta cgccgatgca cccgtcgtgc gggccgagtg cctcaaggac
     1861 gtcctcgagc gcgcgctgcc gtactggcac agcgacatcg tcccagacct gcaggccggc
     1921 aagtcggtgc tcgtcgccgc gcacggcaac tcgatccgcg cgatcgtcaa gtacctcgac
     1981 gacatcgacg acgcgaccat cgcggggatc aacatcccca cgggcatccc gctgcg
//
LOCUS       DASRKH010000453         2380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_56944, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000453 DASRKH010000000
VERSION     DASRKH010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56944"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VFC06_07265"
     CDS             <1..301
                     /locus_tag="VFC06_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZL81713.1"
                     /translation="CMLGEPIGAQAALDMGMVYEVAPADEAYARARALADKIAEKSPV
                     GINHVKQISNRLHDYSMDTYFQIEGDYLALGALSADFIETNVAAAQKRAPEYKGF"
     gene            complement(444..917)
                     /locus_tag="VFC06_07270"
     CDS             complement(444..917)
                     /locus_tag="VFC06_07270"
                     /inference="COORDINATES: protein motif:HMM:NF039500.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5680 domain-containing protein"
                     /protein_id="HZL81714.1"
                     /translation="MTTTDLDDLTRFIVRAKAHTYAASSGIVASSRPGSHDLQFTDGP
                     FTYIDTYVGGADFLGQELVVRDRQPVWALNYYGYLLRPDLIDAATSGATIKACLTGLY
                     AEGRFLGGWHFEVDPYTYTDTNDGEVTHFTGTEWIDRDGVRVYELVYHGGLVRER"
     gene            complement(1066..1944)
                     /locus_tag="VFC06_07275"
     CDS             complement(1066..1944)
                     /locus_tag="VFC06_07275"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018771928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZL81715.1"
                     /translation="MTTTGLPLPLAATGLIAVTGATGHVGGLVAHDLSREGARLRLVV
                     RSAARAPQLPGATVTETTYGDHDRSVVALDGVDVLLMVSAAESADRLDQHRAFIDAAV
                     EAGVHHVVYTSFLGAAPDATFTLARDHWATEEHLRAVGVPHTILRDSFYLDFLPNLAG
                     PDGAIRGPAGDGVLGAVARADVARVAAHVLLAPHEHAGQTYELTGREALSLTQAARIT
                     SEVTGRETVYHPETVEEAYASRASYGAPDWQVDAWVSTYTAIAAGELALVTDTVERLT
                     GRRPLTLRELLAPLAL"
     gene            1988..>2380
                     /locus_tag="VFC06_07280"
     CDS             1988..>2380
                     /locus_tag="VFC06_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZL81716.1"
                     /translation="MPSAAPEWFDGQMNGSPVEAVLLTDARLVGAARPVDVLVRDGRI
                     EAVGPRAAVDGRAGVERIDLGGRTVVPGLWDAHTHMTQWALARRRVDVSGASSAAEAV
                     AIVARRLAERPPPDGVPLVGHGFRDGLWP"
BASE COUNT          323 a          819 c          905 g          333 t
ORIGIN      
        1 gtgcatgctc ggcgagccga tcggtgcgca ggccgcactc gacatgggca tggtctacga
       61 ggtcgcgccg gccgacgagg cgtacgcccg ggcccgcgcc ctggccgaca agatcgccga
      121 gaagtcgccc gtcggcatca accacgtcaa gcagatctcg aaccggctgc acgactactc
      181 gatggacacg tacttccaga tcgagggcga ctacctcgcg ctgggtgcgc tgagcgcgga
      241 cttcatcgag acgaacgtcg ccgcggcaca gaagcgggct ccggagtaca aggggttctg
      301 agcccggggg agaacgacga cgacggcggc gccggaacct tcgggtaccg gcgccgccgt
      361 ggacgctcgc gacgcccgcg ttaaggaacg cctgggtaca gcgccgcctc cgcctgcgac
      421 gcccagcccg gtggcgcctg ccgtcagcgc tcgcgcacga gcccgccgtg gtagacgagc
      481 tcgtagaccc gcacgccatc ccggtcgatc cactccgtgc cggtgaagtg cgtgacctcg
      541 ccgtcgttcg tgtcggtgta cgtgtagggg tcgacctcga agtgccagcc gcccaggaag
      601 cggccctcgg cgtacagccc cgtgaggcac gccttgatcg tcgcgcccga tgtggccgcg
      661 tcgatgaggt ccggacgcag caggtacccg tagtagttga gcgcccagac gggttgcctg
      721 tcccgtacga cgagctcctg cccgaggaag tccgcgccgc ccacgtatgt gtcgatgtag
      781 gtgaacggtc cgtcggtgaa ctgcaggtcg tgcgacccgg gacgggacga cgcgacgatg
      841 ccactgctgg ccgcgtacgt gtgcgccttc gcccggacga tgaatcgcgt gaggtcgtcg
      901 aggtcggtcg tcgtcatggg tgctccaggg tcgtcgcggg tctcggtgca gaggctagga
      961 cggggcaccg acacggacga acatcgcggc ggccaagaag cgcgcgccgg tgtaccgagg
     1021 gttctcgggg cttggtgccc ccgacgcgcc tcccgtcggg cgcagtcaga gcgcgagcgg
     1081 tgccagcagc tcgcggagcg tcagcggccg acggcccgtg agccgctcga ccgtgtcggt
     1141 gacgagggcc agctcgccgg cggcgatcgc ggtgtaggtc gagacccagg cgtcgacctg
     1201 ccagtcgggg gcgccgtagg acgcgcgtga cgcgtacgcc tcctcgaccg tctcggggtg
     1261 gtacaccgtc tcgcggccgg tcacctcgct cgtgatccgg gctgcctggg tgaggctcag
     1321 cgcctcgcgt cccgtgagct cataggtctg cccggcatgc tcgtgcggcg cgaggagcac
     1381 gtgcgcggcg actcgtgcga cgtcagcgcg agccacggca ccgagcacgc cgtcgcctgc
     1441 cgggccccgg atcgcgccgt cgggcccagc gaggttcggc aggaagtcga ggtagaagct
     1501 gtcgcgcagg atcgtgtgcg gcacgccgac ggcgcgcagg tgctcctcgg tggcccagtg
     1561 gtcgcgtgcg agcgtgaagg tcgcgtccgg cgcggcgccg aggaacgacg tgtagacgac
     1621 gtggtggacc ccggcctcga cggccgcgtc gatgaaggcg cggtgctggt cgagccggtc
     1681 ggcgctctcc gcggcggaga ccatgagcag cacgtcgacg ccgtcgagcg cgacgaccga
     1741 ccggtcgtgg tcgccgtagg tcgtctccgt gacggtcgcg ccggggagct gcggcgcccg
     1801 cgcggccgac cggacgacca gccgcagccg agccccctcg cgcgacaggt cgtgggcgac
     1861 gagcccgccg acgtggccgg tcgcgccggt cacggcgatg agccccgtcg cggcgagagg
     1921 aaggggcaag cccgtcgtgg tcatccctcc atgatggatc ggtgccgccg cgagtgcctg
     1981 ccacgccgtg ccgtccgctg cccccgagtg gttcgatggc cagatgaacg gatctccggt
     2041 cgaggctgtc ctgctcacag acgcgcggct cgtgggtgcg gcgcgcccgg tcgacgtgct
     2101 cgtgcgcgat ggccggatcg aggcggtcgg acctcgggcc gcggtggacg gccgagcggg
     2161 tgtcgagcgg atcgacctcg gcggccggac ggtcgtcccc ggcctgtggg acgcacacac
     2221 gcacatgacg cagtgggcgc tcgcgcggcg tcgggtcgac gtgtccgggg cgtcgtcggc
     2281 cgctgaggcc gtcgcgatcg tcgcccgtcg gctcgccgag cggccgccgc ccgacggcgt
     2341 gccgctcgtc gggcacggct tccgcgacgg gctgtggccc
//
LOCUS       DASRKH010000454         2768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_56967, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000454 DASRKH010000000
VERSION     DASRKH010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56967"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /gene="atpB"
                     /locus_tag="VFC06_07285"
     CDS             <1..828
                     /gene="atpB"
                     /locus_tag="VFC06_07285"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015881800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HZL81717.1"
                     /translation="VFDAAAAALSRGVLPLSEDGGGGFHGPTAEDFFPPAIFGEGTFY
                     EFNRIMMVRVIATVALCVLFLLAARNVKLVPGRGQGIAEIALDFVRVNVSEEVLGKER
                     GKAWVPLLSVIFFGVFAMNITGVVPGLNIAGASVVGFPLVVAVVAWIAFMVAGIRAHG
                     AWHFYRDQIFTPGVPWPLYFILAPIELVSTFLLRPLTLTIRLLANMMSGHFLLVLCFA
                     ATNALFLEASPPLKPLGLLTLGASFAFYGLEIFIAALQAYIFALLTAVYISLSVESH"
     gene            900..1109
                     /gene="atpE"
                     /locus_tag="VFC06_07290"
     CDS             900..1109
                     /gene="atpE"
                     /locus_tag="VFC06_07290"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019134875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HZL81718.1"
                     /translation="MNITGSIATIGYGLATLGPGIGLGILVGKTQEATARQPEMAGPL
                     RINMFIGMALVEALGLIGLAAGFLF"
     gene            1119..1658
                     /locus_tag="VFC06_07295"
     CDS             1119..1658
                     /locus_tag="VFC06_07295"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HZL81719.1"
                     /translation="MAPVEVPGNILLPAVYDIFWSAVCFAVIAFVLARKVLPTFTAML
                     DERAVKIEGGLKAGEIAREEAATLRQGLEAERAEARREAALIREGANDDARQIVAEAR
                     QKAVVEAERISANAARSIRAERQAAAVSLRTDVGLLATELASRIVGESLTDAELQSRV
                     IDRFIDELDALTPVAVEET"
     gene            1658..2470
                     /locus_tag="VFC06_07300"
     CDS             1658..2470
                     /locus_tag="VFC06_07300"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HZL81720.1"
                     /translation="MRASSEASLQAAKERWNAVLETSDAVDTLKVELTHVVDAIDSSP
                     GLRRALTDPARRGEDKAALVADVFGKGFNPDIVDLVSGMARARWSHADDLSTALERLG
                     AETALASAERAGELDRVADELFQVFRLLGHERQLRNELTETDDSPERRLALLDAVFGK
                     YLLPRTMQILGRTVTSSRHPSVTSSILETAQFAAERRDRLVAVVTAAVPLTDAQIARL
                     EATLGRKYNRNVHINVAVDPQVVGGIRVHIGHNLVDNTVAKQVETIRRAFAD"
     gene            2515..>2768
                     /locus_tag="VFC06_07305"
     CDS             2515..>2768
                     /locus_tag="VFC06_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019134872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HZL81721.1"
                     /translation="MSELTIKPEDIRAALNSFVNSYEPAQAVTEEVGRVTVTADGIAR
                     VEGLPGTMANELLRFADGTEGLAMNLDVREIGVVVLGEFTG"
BASE COUNT          430 a          952 c          910 g          476 t
ORIGIN      
        1 gtgtttgatg ccgcagctgc cgcgctgagc cgaggtgtcc tcccgctgtc ggaggacggg
       61 ggcgggggtt tccacggtcc cacggccgag gacttcttcc cgcccgcgat cttcggggag
      121 ggaaccttct acgagttcaa ccgcatcatg atggtgcgtg tcatcgcgac cgtcgcgctc
      181 tgcgtgctct tcctgctcgc ggcacgcaac gtgaagctgg tcccgggccg cggccagggc
      241 atcgccgaga tagcactcga cttcgtgcgc gtcaacgtct ccgaggaggt cctcggcaag
      301 gagcgtggga aggcctgggt gcccctcctc tcggtcatct tcttcggtgt gttcgcgatg
      361 aacatcaccg gcgtggtccc cgggctcaac atcgccggtg cgtccgtggt cggcttcccg
      421 ctggtggtgg cggtcgtcgc ctggatcgcc ttcatggtcg cgggtatacg ggcgcacggc
      481 gcctggcact tctaccggga ccagatcttc acccccgggg tgccctggcc cctctacttc
      541 atcctggcgc ccatcgagct ggtctccacg ttcctgctcc ggccgctcac gctcacgatc
      601 cgtctcctgg ccaacatgat gagcgggcac ttcctgctcg tgctctgctt cgcggccacc
      661 aacgccctct tcctggaggc ctcgcccccg ctcaagccgc tgggactcct gacgctgggc
      721 gcgtcattcg ccttctacgg gctcgagatc ttcatcgcgg cgctgcaggc gtacatcttc
      781 gcgctgctga ccgccgtcta catctcgctc tcggtggaga gccactagtt tcccgccgcg
      841 gcgggggaaa cccaggtgcc gggagaaccg gaaccacaca tacggaagga aacctctcca
      901 tgaacatcac cggttccatt gcgacaatcg gttatggcct tgcgacgctc ggccccggca
      961 tcggtctcgg catcctggtc ggcaagaccc aggaggcgac cgcgcggcag cccgaaatgg
     1021 ccggcccgct gcgcatcaac atgttcatcg gcatggccct ggtcgaggcg ctcggtctga
     1081 tcggtctggc cgccggcttc ctcttctaag gactcacagt ggcccccgtc gaagttcccg
     1141 gcaacatcct gctgccggct gtctatgaca tcttctggtc ggcggtctgt ttcgccgtca
     1201 tcgccttcgt gctggccagg aaggtcctgc cgacattcac ggcgatgctc gacgagcgtg
     1261 cggtcaagat cgagggagga ctgaaggccg gggagatcgc ccgcgaggag gctgcgacgc
     1321 tgcgccaggg cctcgaggcc gaacgcgccg aggcgcgcag ggaggcggcc ctcatccgtg
     1381 agggcgccaa tgacgatgcc cgccagatcg tcgcggaggc ccggcagaag gccgtcgtcg
     1441 aggccgaacg catcagcgcc aacgccgcga ggtccatcag ggccgagcgg caggctgccg
     1501 cggtctccct gcggaccgat gtcggcctgc tcgccacgga gctcgcatcg cgcatcgtgg
     1561 gcgagtccct caccgacgcc gaactccagt cgcgcgtgat agaccgcttc atcgacgagc
     1621 tcgacgcctt gaccccggtg gcggttgagg agacctgatg cgcgccagca gcgaagcgtc
     1681 actccaggcc gccaaggagc ggtggaacgc cgtcctggag acctcggacg cggtggacac
     1741 cctgaaggtg gagttgaccc acgtcgttga cgcgatcgat tcctcgcccg ggctgcgtcg
     1801 tgcgttgacc gatcccgccc gtcgcggcga ggacaaggcc gccctggtgg ccgacgtgtt
     1861 cggcaagggt ttcaatccgg acatcgtgga cctcgtgtcc ggcatggccc gtgctcgttg
     1921 gtcgcacgcg gacgacctgt ccaccgcgct cgagcggctc ggcgccgaga ccgcgctggc
     1981 gagcgccgag cgcgccggcg agctggaccg cgttgcggat gagctgttcc aggtgttccg
     2041 gttgctcggc cacgagcgcc agttgcggaa cgagctcacc gagaccgacg actcgccgga
     2101 gcggcgtctg gcgctcctcg acgcggtctt cgggaagtac ctgctgcccc gcaccatgca
     2161 gatcctcgga cgcaccgtga cctcgtcccg ccacccgtcg gtgacctcgt ccatcctcga
     2221 gaccgcccag ttcgccgcgg aacgacgtga ccggctcgtc gccgtcgtca ctgccgccgt
     2281 gccactgacg gacgcgcaaa tcgcccggct ggaggcgaca ctcggccgca agtacaaccg
     2341 gaacgtgcac atcaatgttg ccgtcgaccc gcaggtggtg ggcgggattc gcgtgcacat
     2401 cggccacaac ctcgtggaca acacggtggc gaagcaagta gagaccattc ggcgcgcgtt
     2461 cgccgactga caacgagaac cacgaccgcc gggtcgagaa gttgaggaat cgcaatgtcc
     2521 gaattgacga tcaagccgga ggacatcagg gctgccctga acagcttcgt gaactcctac
     2581 gagccggcgc aggccgtgac cgaggaagtc ggtcgtgtga cggtcacggc tgacgggatc
     2641 gcccgggtcg aaggcctccc gggcaccatg gcgaacgaac tgttgcgctt cgccgacggc
     2701 accgagggtc tcgcgatgaa cctcgacgtt cgcgagatcg gcgtcgtcgt cctcggcgag
     2761 ttcaccgg
//
LOCUS       DASRKH010000455         2027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_42982, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000455 DASRKH010000000
VERSION     DASRKH010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42982"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..392)
                     /locus_tag="VFC06_07310"
     CDS             complement(63..392)
                     /locus_tag="VFC06_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZL81722.1"
                     /translation="MPIVVHQAKAELFRTLGHPARIRILELLTEREHRVHELLAAMEI
                     EQSNLSHQLAVLRRAGLVVQRREGADVVYATSVPTVTELLRVARVLLAELLAGQEPLK
                     VELEEIP"
     gene            809..1789
                     /locus_tag="VFC06_07315"
     CDS             809..1789
                     /locus_tag="VFC06_07315"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZL81723.1"
                     /translation="MIDELGLTGLQWELYRALLWNPRTEPRELAAALDVDTASLEADL
                     RRLELVGLAVGSTSSPSRFRPVYPQVGLQRLLHQKLDQHDEEGHLLRELSAAIAEFGD
                     EFAAHRREYEVSLLNEVTDPGEMASRVNELVRDSTSEILSFVTNTFSEASLTHARVGD
                     QEVLDRGVRARSIYLDAAVEEGPTRDYLHWLESQGAFVRSAPSLPVRMIIFDGAAAVV
                     SRRALDPGHGALVVQSQGLVAALVSLFEAYWGHARPLSGAGPGPGVRLSAVEHDILTL
                     LAQGMKDDAIAHRLELSVRTVRRALNQLYERTGSGSRFELGVKATRFGWL"
     gene            1800..>2027
                     /locus_tag="VFC06_07320"
     CDS             1800..>2027
                     /locus_tag="VFC06_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HZL81724.1"
                     /translation="MRALMKPYAGPGLELRDIPEPTPGPGEVKIRVIRAGLCGTDLHI
                     QDWDDWSASMLHPPMVIGPDLHDLAVELVPDH"
BASE COUNT          328 a          711 c          674 g          314 t
ORIGIN      
        1 cgggggcgcg ggctcggcga tccggccggt cctcctgatg aggcgcacga gggcggagag
       61 gctcacggga tctcctcgag ctcgaccttg aggggctcct gccccgcgag cagctcggcc
      121 agcaggacgc gggccacccg gagcagctcg gtgacggtcg ggacgctggt ggcgtagacg
      181 acgtcggcac cctcgcggcg ctggaccacc aacccggcgc ggcggaggac ggcgagttgg
      241 tgcgagaggt tggactgctc gatctccatc gccgcgagca gctcgtgcac gcgatgctcg
      301 cgctccgtca gcagttcgag gatgcggatc cgggcggggt gcccgagcgt gcggaagagc
      361 tccgccttcg cctggtgcac cacgatgggc atcgacaccc tcctcccgat gcgaacagat
      421 tagcaactga acaactcttc atgttcagga atggtgccgg catggaaaga gcccccgggg
      481 cggggcccac ggaggcttcg acctgaaaaa ggccgcgttg atatgcgaac cgaccggggt
      541 cggccaggtc gccggcgatc tacctggccc gcatctccca gcaatcaatg cacatcgggc
      601 ggatcgagcc cgccttgtcg acacttacga ggccgccaaa gaccagcaaa cccgcgacac
      661 tcagtgccgc aacgatcttc cggaacatta ccgtcaactc ccctgctact ctgcaacagc
      721 gtttcggtgg gggacaccca cctgctccct atagtagtgg cagtgaccca tttttgaccc
      781 ccggggcaca cgacttcctg gaggcgcggt gatcgacgaa cttggtctca ccggcctcca
      841 gtgggagctg taccgggcgc tgctctggaa tccccggact gaaccgcgtg agctcgccgc
      901 cgcactggac gtggacacgg cgtcgctcga agcggacctc aggcgactgg agctggtggg
      961 cctggcggtg gggtccacgt cctcccccag ccgctttcgc ccggtctacc cgcaggtggg
     1021 cctgcaacgc ctgctccacc agaagttgga ccagcacgac gaggaaggcc acctgctcag
     1081 ggagctctcg gcggcgattg ccgagttcgg tgacgagttc gccgcgcacc ggcgggaata
     1141 cgaggtctcc ctcctgaacg aggtcacgga ccctggggag atggcctccc gcgtcaacga
     1201 actcgtgcgg gactccacgt cggagatcct gtccttcgtc acgaacacct tcagcgaggc
     1261 ctccctcacc cacgcccgcg tgggtgacca ggaggtgctc gaccgggggg tgcgcgcccg
     1321 cagcatctac ctggacgctg ccgtggagga ggggcccacc cgcgactacc tgcactggct
     1381 cgagtcgcag ggcgccttcg tccgctccgc cccgagcctc ccggtccgga tgatcatctt
     1441 cgacggcgcc gccgccgtgg tctcccgccg cgcgctcgac cccgggcacg gtgcgctcgt
     1501 cgtgcagtcc cagggactgg tcgccgccct ggtcagcctc ttcgaggcgt actggggcca
     1561 tgcccggccc ctctccgggg cggggcccgg ccccggggtg agactctccg cggtcgagca
     1621 cgacatcctc accctgctcg cccaaggcat gaaggacgac gccatcgccc atagactcga
     1681 actgtcggtg cgaacggtgc gccgcgcact caatcagctc tacgagcgca ccggctcggg
     1741 gagccgcttc gaactggggg tcaaggccac ccggttcggc tggctgtagg gagtcacaca
     1801 tgcgggcgtt gatgaagccg tacgcgggtc cggggctcga actgcgtgac atccccgaac
     1861 ccaccccggg tccgggcgag gtcaagatcc gggtgatccg cgcggggctg tgcggcaccg
     1921 acctgcacat ccaggactgg gacgactggt ccgcgtcgat gctgcacccg cccatggtga
     1981 tcgggcccga tctccacgat ctcgccgtag aactcgtgcc cgatcac
//
LOCUS       DASRKH010000456         3203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_32668, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000456 DASRKH010000000
VERSION     DASRKH010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32668"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="VFC06_07325"
     CDS             <1..514
                     /locus_tag="VFC06_07325"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZL81725.1"
                     /translation="NRETPEFVTEMLTAEHELDHFDSGEAETDTSLRRNALRAQQQGS
                     ARTRVLSRVGHHRVLGYYAISPHDTHREDVPSAAAGGLRIVRGYLVAQLAVDRSLQGQ
                     GNGGELLLDALVTIASAAQTVGGRLVVVDSVNESAVAFYEHHGFIRIGDSRRLYQKIP
                     RIQLSLTTDS"
     gene            648..827
                     /locus_tag="VFC06_07330"
     CDS             648..827
                     /locus_tag="VFC06_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81726.1"
                     /translation="MTSWPLTSREAWSSMISTWVTASVRNFSVDLVTGRPPRPEPVPA
                     RRRNVGAAPGLVPVE"
     gene            complement(892..2886)
                     /gene="glgB"
                     /locus_tag="VFC06_07335"
     CDS             complement(892..2886)
                     /gene="glgB"
                     /locus_tag="VFC06_07335"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF008967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HZL81727.1"
                     /translation="MAYDVTGGLTGWDLEGFHQGGDTECWKRLGAHLVTVQDDERGEI
                     TGTRFSVWAPNAHAVRIKADFNWWTGDDMHLVPGSGVWALFVEGVGEGTMYKFDILGA
                     DGIWHEKVDPMASFAERAPSNASIVYESKYLWDDDAWIAKRAAHVPHESPMSIYEVHL
                     GSWRRGKTYLELADELVEYVTWQGFTHVELMPVTEHPLEASWGYQVTNYFAPMSRLGR
                     PDEFRYLVDRLHQADIGVILDWVPGHFPKDEWALGRFDGTALYEHADPRQGEHKEWGT
                     YIFNYGRNEVKSFLVSNALYWATEFHIDALRLDAVASMLYLDYDREDGEWVPNIHGGN
                     HNLEAVDFLRYVNMHLYERVPGVIMIAEESTAFSGVTKPVDMGGLGFGFKWNMGWMHD
                     SLGYIGEEPIHRQYHHHEMTFAMVYQYSENYVLPISHDEVVHGKGSMINKIPQDDWRK
                     FGTLRAFYSFMWSFPGKKLVFMGCEFGQRAEWNEANSLEWWVDGLWGHHGLQLMFKAL
                     NELYQAHPALWKLDTDPQGFRWINGDDAGANTFSWLRFDGDGEQIACAINFSSEPWTN
                     YRIGLPETGTWTEVFNSDNSTYDGTGSFGNLGKVTAVDEPYYGFPASATIVVPPLGAV
                     FFRYEPGAPAAHQASLTSAVEDVASPNSHNSPDSAEEPEA"
BASE COUNT          502 a         1050 c         1092 g          559 t
ORIGIN      
        1 gaatcgggag acgcccgaat tcgtcaccga gatgttgacg gccgagcatg aactcgacca
       61 cttcgacagc ggtgaagcgg agacggatac ttcgttgcgg cggaacgcgc tgcgcgccca
      121 gcagcaggga agcgcacgca cccgcgtact cagccgcgtc gggcaccacc gagtgctggg
      181 gtattacgcg atcagtccgc acgacaccca ccgggaggac gtgcccagcg cggccgctgg
      241 tggtctgcgt atcgtgcgtg gctacctggt cgcgcaactc gccgtggacc gcagcctgca
      301 aggtcaggga aacggtgggg agttgctgct ggacgccctg gtcacgatcg cctccgcggc
      361 gcagaccgtc ggcgggcgac tggtcgtggt cgactccgtg aacgaaagcg cggtggcctt
      421 ctacgagcat catggcttca tccgaattgg tgacagccgt cggctgtacc agaagatccc
      481 ccggatccag ctctccctca ctaccgacag ctgagtcgtc cggccctacg cagccaaacc
      541 tgaccccttc cgtgcactcc agctcgccgc ccgtcagcac gcgggaggca gggtcgatac
      601 ggctcagcac ttccaggccc gcgtacagtc ccacgcacag cgcgtcgatg accagttggc
      661 cgctgacgtc gcgcgaggcg tggtcgagca tgatctcgac gtgggtcacg gcttcggtcc
      721 gcaacttctc cgtcgacctg gtcacagggc gaccgcctcg gcccgaacct gttcccgcac
      781 gacgacgcaa cgtgggcgcc gccccggggc tcgtgcccgt ggagtagccg gaccggcatg
      841 tgccggacag ggcgacggcg cctcacctcg gtggggcgcc gtcgtggttg gtcaggcctc
      901 gggctcctcc gcgctgtcgg ggctgttgtg gctgttgggg ctcgcgacgt cctcgaccgc
      961 cgacgtgagc gaagcctggt gcgctgccgg ggcgccgggc tcgtaccgga agaacacggc
     1021 gcccagcggc gggacgacga tggtggcgct ggccgggaag ccgtagtacg gctcgtccac
     1081 cgcggtcacc ttgccgaggt tgccgaagga cccggtgccg tcgtaggtcg agttgtcgga
     1141 gttgaagacc tccgtccagg tcccggtctc gggcaggccg atccggtagt tggtccacgg
     1201 ctccgaggag aagttgatcg cgcaggcgat ctgttcgccg tcgccgtcga agcggagcca
     1261 ggagaaggta ttggcgccgg cgtcgtcgcc gttgatccac cggaacccct gggggtccgt
     1321 gtcgagcttc cacagggcgg ggtgggcctg gtacagctcg ttgagggcct tgaacatgag
     1381 ctggagcccg tggtggcccc agagcccgtc cacccaccac tcgaggctgt tggcctcgtt
     1441 ccactcggcg cgctgcccga actcgcagcc catgaacacc agcttcttgc ccgggaagga
     1501 ccacatgaag gagtagaacg cgcgcagggt gccgaacttg cgccagtcgt cctgggggat
     1561 cttgttgatc atggacccct tgccgtggac cacctcgtcg tggctgatgg gcaggacgta
     1621 gttctcggag tactggtaca ccatcgcgaa ggtcatctcg tggtggtggt actgccggtg
     1681 gatcggctcc tcgccgatgt agcccagcga gtcgtgcatc cagcccatgt tccacttgaa
     1741 cccgaagccc aggccgccca tgtccaccgg cttcgtgacg ccgctgaacg cggtggactc
     1801 ctcggcgatc atgatcacgc cgggcacccg ctcgtacagg tgcatgttga cgtaccgcag
     1861 gaagtcgacg gcctccaggt tgtggttgcc gccgtggatg ttcggcaccc actcgccgtc
     1921 ctcgcggtcg tagtccaggt agagcatcga ggcgacggcg tccagccgga gcgcgtcgat
     1981 gtggaactcc gtggcccagt agagcgcgtt ggagaccagg aacgacttca cctcgttgcg
     2041 gccgtagttg aagatgtagg tgccccactc cttgtgctcg ccctggcgcg ggtcggcatg
     2101 ctcatagagt gcggtgccgt cgaaccggcc cagcgcccac tcgtccttgg ggaagtgacc
     2161 ggggacccag tcgaggatca cgccgatgtc cgcctggtgg aggcggtcca cgaggtaccg
     2221 gaactcgtcg gggcggccga gccgggacat cggggcgaag tagttggtca cctggtagcc
     2281 ccaggaggcc tcgagagggt gctcggtgac cggcatgagc tcgacgtggg tgaagccctg
     2341 ccacgtcacg tactcgacca gttcgtcggc cagttcgagg taggtcttgc cgcggcgcca
     2401 cgagccgagg tgcacctcgt aaatcgacat cggcgactcg tgcggcacgt gggcggcccg
     2461 cttggcgatc cacgcgtcgt cgtcccagag gtacttgctc tcgtacacga tggaggcgtt
     2521 cgacggcgcc cgctcggcga agctcgccat cgggtccacc ttctcgtgcc agatcccgtc
     2581 ggcaccgagg atgtcgaact tgtacatggt gccctcgccg actccttcga cgaagagcgc
     2641 ccacaccccg gagccgggaa cgaggtgcat gtcgtcgccg gtccaccagt tgaagtccgc
     2701 cttgatccga acggcgtggg cgttgggggc ccagaccgag aagcgggtgc ccgtgatctc
     2761 gccacgttcg tcgtcctgca ccgtaaccag gtgtgctccg aggcgcttcc agcactccgt
     2821 gtccccgccc tggtggaagc cttcgaggtc ccagccggtc aatccaccgg tcacgtcgta
     2881 tgccatcaaa tcctccgcgt cgggttggcc cgggctgctt ggtcgtcccg gttggtgtcc
     2941 ataatggcgt aagagtagtc acagtagggt gggagtcggt gccgctttcg gcgtgcgctg
     3001 ccttgccggt cccgttggtt gccgctctcc gggctcaccg ggagaggcaa cgagtggcgc
     3061 agatccatgc ggggaccgtc tgggaggata gcgggctcgt cttcaccacg ctcaccggca
     3121 agctgatcgc acccggcgac cactcggcgc actggacggc cattctggag agatcgggcg
     3181 tgcggcccgc tcgactccac gac
//
LOCUS       DASRKH010000457         2183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_35807, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000457 DASRKH010000000
VERSION     DASRKH010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35807"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VFC06_07340"
     CDS             complement(<1..189)
                     /locus_tag="VFC06_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HZL81728.1"
                     /translation="MTAVVLAFALVGCASIGRPDAGSQGAGYEAGDGSFTTWDTAERG
                     QGIVLTGTTFAGEVLDLED"
     gene            complement(207..929)
                     /locus_tag="VFC06_07345"
     CDS             complement(207..929)
                     /locus_tag="VFC06_07345"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HZL81729.1"
                     /translation="MPQSTVHLMRHGEVFNPEAILYGRMPGFGLSRRGRRMTERVGEV
                     LHSGGHDIRAVVASPLERAQESAAPTAEAFGLPVLTNDDLVEAGNLFEGLAVNRNRLQ
                     LANPKYWPRYRNPFEPSWGEPYAEQVTRMSGAIRSALELVPDGGEVLAVSHQLPIWVT
                     RLFLERRPLAHDLRRRQCSLASLTSLTFDGRRLVALTYWEPAGDLLHGATDMNPGQSA
                     ATVAGAETPEAAGAPEAAGAGE"
     gene            complement(978..1439)
                     /locus_tag="VFC06_07350"
     CDS             complement(978..1439)
                     /locus_tag="VFC06_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZL81730.1"
                     /translation="MGKTAVKEARWLSLEDQAIWRQYLRATLEVRESLERDLLLEFGL
                     SLNEYEVMVVLSESPDHTTRMSTLAEDLVNSRSRLTHTVHRMEKRGLVERTSCSDDRR
                     GVNCRLTDSGYAVLESTAPSHVESVRRNIFDRLSREEIDALGRIMAKLGDV"
     gene            1681..>2183
                     /locus_tag="VFC06_07355"
     CDS             1681..>2183
                     /locus_tag="VFC06_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HZL81731.1"
                     /translation="MSTIPAGTYVIDQSHSEVGFNARHAGIAKVRGQFTEYEGALVVG
                     EDLADSSVTVTIQTASIDTNNEGRDGHLRSADFFDVDSNPTMTFTSTALNGTGEDFTV
                     AGDLTLNGITRPVELEVDYNGSAVDPFGNQRVGFEAKTTVNRKDFGLTWNAALETGGF
                     LVSDKIN"
BASE COUNT          352 a          783 c          725 g          323 t
ORIGIN      
        1 gtcctcgagg tcgaggacct ccccggcgaa ggtggtgccc gtgagcacga tcccctgacc
       61 gcgttccgcg gtgtcccacg tggtgaacga cccgtcgccg gcctcgtaac cggcgccctg
      121 cgaaccggcg tcgggccgcc cgatggaggc acagccgacg agcgcgaacg cgagcacaac
      181 ggcggtcacg cccgcgcgca ctcgcctcat tcgcccgccc cggcggcctc cggcgccccg
      241 gcggcctccg gcgtctcggc gcctgcgacg gtggcggcgg actggccggg gttcatgtcg
      301 gtggcgccgt gaaggaggtc gcccgccggc tcccagtagg tgagcgcgac gagccgccgg
      361 ccgtcgaacg tgagcgaggt cagggaggcg agcgagcact gcctgcggcg caagtcgtgg
      421 gccagcggcc gccgctccag gaagaggcgc gtcacccaga tcggcagctg gtgggacacg
      481 gccagcacct cgccgccgtc gggcacgagt tcgagggccg aacggatcgc cccggacatc
      541 cgcgtcacct gctcggcgta gggctcgccc caggagggtt cgaacgggtt gcggtaccgg
      601 ggccagtact tcggattggc cagttgcagc cggttgcggt tcacggccag cccctcgaag
      661 aggttgcccg cctcgaccag gtcgtcgttc gtgagcacgg gcaggccgaa cgcctccgcg
      721 gtgggcgcgg ccgactcctg ggcgcgctcc agcggcgagg cgacgacggc gcgaatgtcg
      781 tggccgccgg agtggagcac ctcccccacc cgttccgtca tgcggcggcc acggcgcgag
      841 agcccaaagc ccggcatgcg gccgtagagg atcgcttcgg gattgaagac ctcgccgtgg
      901 cgcatcaggt ggaccgtcga ctgtggcatg ccccaagtct tacagactcg cgccgatctg
      961 cgagtccggc ccgggactca gacgtccccc agcttggcca tgatccggcc gagggcatcg
     1021 atctcctcgc gggacagccg gtcgaagatg ttgcgccgaa cggactccac atgggacggg
     1081 gcggtggact cgagcaccgc gtacccggag tcggtgagcc ggcagttcac gccgcgacgg
     1141 tcatcgctgc acgaggtccg ctccacgaga ccgcgcttct ccatccggtg cacggtgtgc
     1201 gtgaggcggg aacgcgagtt caccaggtcc tcggcgagcg tggacatccg cgtcgtgtgg
     1261 tccggggact cggacagcac caccatgacc tcgtactcgt tgagtgacag gccgaactcc
     1321 aagagcaggt cgcgctcgag cgactcccgc acctccagcg tggcgcgcag gtactgtcgc
     1381 cagatggcct ggtcctcgag cgagagccac cgggcttcct tcacggccgt tttccccatt
     1441 gcgtgtcctt gtgtggggag cactggccgc cccgggcgca tgcgccgggc gatccaccac
     1501 tccaattcat tgatcgttca atcataacgc gtccggccgc gtccggcacc cccatgagcc
     1561 gcgtgcccgg tgtggagctg gctgtgtccc ggctcacgtt cccagccgtt gcattgttga
     1621 atgttcaacc atatgctgga ggagttccaa gcaatccgga tccaccccag gagacacacc
     1681 atgagcacca tccccgcagg cacctacgtc atcgaccagt cccactccga ggtcggcttc
     1741 aacgcccgcc acgccggcat cgccaaggtt cgcggccagt tcaccgagta tgagggcgcc
     1801 ctcgtggtgg gcgaggacct cgccgactcg tccgtgaccg tcaccatcca gaccgcctcg
     1861 atcgacacca acaacgaggg ccgcgacggg cacctgcgct ccgccgactt cttcgacgtc
     1921 gattccaacc cgaccatgac gttcacctcc accgcactga acggcacggg cgaggacttc
     1981 accgtggcgg gcgacctcac cctgaacggc atcacccggc cggtcgagct cgaggtcgac
     2041 tacaacggct ccgccgtcga cccgttcggc aaccagcgcg tgggcttcga ggcgaagacc
     2101 accgtcaacc gcaaggactt cggcctgacc tggaacgccg cgctcgagac cggcggcttc
     2161 ctggtgagcg acaagatcaa cat
//
LOCUS       DASRKH010000458         3485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_52577, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000458 DASRKH010000000
VERSION     DASRKH010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52577"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VFC06_07360"
     CDS             <1..310
                     /locus_tag="VFC06_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81732.1"
                     /translation="AENPSVSIALLDQLAHVGWGSARPPVKSSPLAPPRLMATIAATA
                     DTEVRDSAIRVINKRLSEPLGVGKRNVGALEMLRDQEWWTLNPESPEVAVSLALFPNV
                     "
     gene            462..893
                     /locus_tag="VFC06_07365"
     CDS             462..893
                     /locus_tag="VFC06_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81733.1"
                     /translation="MTKEVALPPEMRLHVAADPDTAPLALAALAKSRNGQVRSRVALN
                     PSSPPSALATLINTRDVLLQSQVLTHPSLPPRFAAHLARTGDSYIRPKALAKISVMME
                     SLLGIPANNVSARDMLVGEQWWEMEATDPAVVLAKTLFPDA"
     gene            1054..1623
                     /locus_tag="VFC06_07370"
     CDS             1054..1623
                     /locus_tag="VFC06_07370"
                     /inference="COORDINATES: protein motif:HMM:NF013938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81734.1"
                     /translation="MTENEQIRLAMEEDTPPEALDWMTGSEWLGVILEVGSNPSTPPA
                     TLTRLAADDDFAVRVAVARNKAVPLDMLERLAGDADEDVREAVVTNDLATAQILNDLS
                     ERGPVRVRRLAARHANVADATLLALAQSNVVDVQRAAAHSIDERIAMRCGVNAENTSA
                     IEALREQAWWEMTPESPEVAVSLALFPDT"
     gene            1771..3165
                     /locus_tag="VFC06_07375"
     CDS             1771..3165
                     /locus_tag="VFC06_07375"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HZL81735.1"
                     /translation="MGTFRAVRWESGVAGLGMPRKHQRSGTYKAYIPSPLVGQRLDVS
                     PSAAAALSEAERLCAALDASLATRQKSGEAPLAMLRFLEGIGSSAMEGYRSAAARLVA
                     SMATGGSGAKGADMKIVGNLAALDEAHALADVDKITVDDLVNMQAELMNARGESSLVG
                     VREEQNWVGGSDYHPLDAGHVPPPPDLVDALMDDLCEFVSAPSSTPPLLRAALAHAQF
                     ETIHPFLDGNGRTGRALVHLMLRRDGLLDHTVLPLSGTWGRDKPRYIEYLNALRTQGD
                     GWGTDTLDAAAHFMADSTTEAATGAYTIAAAVQGVETGMEKSVAENFRADSVAHRVVH
                     DVCVNLGVTVDGVAARHGADPAAVLRALDRMEGLEIVKRRSVQRPHVFYAPEMVRLVE
                     RFAAQIPEGVVEGKGTPDTGALLTIATAEGLLTSGETKGRCGEWMPRARATCALSKGH
                     PGWPTSGHRQGGAA"
     gene            3201..>3485
                     /locus_tag="VFC06_07380"
     CDS             3201..>3485
                     /locus_tag="VFC06_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81736.1"
                     /translation="MSGCTAPRLGHGTSGARQKCPVHGRPMRPADRAAAKTALGSMAP
                     PLWQTATDPKESPEVLAILAVEGKQDVRERVACNPRASHETLELLAKDPEK"
BASE COUNT          590 a         1127 c         1206 g          562 t
ORIGIN      
        1 ggcggagaac ccgtccgtgt cgatcgcctt gctcgatcaa ctggcccacg tcggctgggg
       61 ctcggcacgc cctcccgtga agtcctctcc gctggcgcct ccgcggttga tggctacgat
      121 tgccgccacc gcggacacgg aggtgcggga ttccgcgatc cgagtaatca acaagcggct
      181 atccgagcct cttggggtag gcaaacgcaa cgtcggcgca ctcgagatgc tgcgtgacca
      241 ggagtggtgg acgctgaatc cggagtcgcc agaggtcgcc gtgtctttgg cgctgttccc
      301 gaacgtgtag ccgtggatac cgttgttgca tgaacaggtg cacggctccg gcgaagggac
      361 acagcagcca gagtggtgcc gccaagtgcc cggcctgccg tgtgtcctcc gtgccgaaca
      421 tggtcccggc cacccctccg atgtcaccga agcaatggtc ggtgaccaag gaggttgcac
      481 tcccgcccga gatgagactc catgtagcgg ccgatccgga caccgcaccg ctggcgcttg
      541 ccgctctcgc gaagtccagg aatggtcagg tgcggtcgag ggtggcgctg aacccgtcgt
      601 caccgccgtc agcacttgcg accctcatca acacgaggga cgtcttgctg caatcccagg
      661 tgctgacgca cccgtcgctt ccgccgcgtt tcgccgccca cttggcgcgg accggggatt
      721 cctacatccg gcccaaggcg ctggcgaaga tctccgtgat gatggagagc ctgctcggga
      781 ttccggccaa caacgtctct gcgcgagaca tgctcgtggg ggagcagtgg tgggagatgg
      841 aagcaaccga tcccgctgtg gtgctggcca aaacgctgtt cccggacgcg tgacgcgatg
      901 accgcaaact gcaccgcccc gatagagggc caccgcgtac cggggtccgc agcgaactgt
      961 cccgtccacg cgccgcgacg ccctgtgcgc gctggcgatc ttcctcgacc tccgctccgg
     1021 gccgcgtcgt tgacccacgc gatggtcgag gagatgaccg agaacgagca aatccgtctc
     1081 gcaatggagg aggacactcc tccggaggcc ttggactgga tgaccggcag cgaatggctt
     1141 ggcgtcatcc ttgaggtggg ttcaaacccg tcaacgccac ctgccaccct gacccgtctc
     1201 gccgccgacg acgacttcgc tgtgcgcgtt gctgttgccc gcaacaaggc ggtgccactg
     1261 gacatgctcg agcgccttgc gggcgacgct gacgaggacg tgagggaggc ggtcgtcacc
     1321 aatgatctcg ccaccgcgca gatactgaat gacctgtcgg agcgcggacc ggtaagggtg
     1381 cgacgtttgg cggcccggca cgccaacgtc gcggatgcga cgctgcttgc tctggcgcag
     1441 tccaatgtcg ttgacgtgca aagagccgca gcccattcaa tcgacgagcg catcgccatg
     1501 cggtgcggtg tcaacgcaga gaacacgagc gctattgagg cgttgcgcga gcaggcgtgg
     1561 tgggagatga cccccgagtc gccagaggtc gccgtctcgc tggcgctgtt cccggacacg
     1621 tgagggcggg atagcgagtc gcccccgcgt tgccctggta ggcgcacggg ggcgcgtttc
     1681 tgccttcgag cgggcgtgcg ctcccaaatc gcacggaaat accctccgta actgacgtta
     1741 gagtgctaaa gtcagttacg gaggcacaac atgggaacgt tcagagcagt ccgctgggag
     1801 tccggcgttg cgggcctcgg catgccacgc aagcaccagc gctcagggac ctacaaggcg
     1861 tacatcccgt cgcccctcgt agggcaacga ctggacgtgt caccctccgc ggccgccgcg
     1921 ctgtccgaag cggagcgcct gtgcgcggcc ctggatgcat ccctggcgac acggcagaag
     1981 tcgggggagg ccccgctggc aatgctgcgg ttcctggaag gcatcggctc gtccgctatg
     2041 gagggctacc gctctgccgc cgcgcgcctg gtcgcgtcaa tggccaccgg cggttccggg
     2101 gccaaaggcg ccgacatgaa gatcgtcggc aacctcgctg ccctcgatga ggcgcacgcc
     2161 ctggcagacg tcgacaagat caccgtggac gacctggtga acatgcaggc ggaactgatg
     2221 aacgcccgcg gtgagtcgtc gctcgtcggt gtccgtgagg agcagaactg ggtgggtggc
     2281 tccgactacc acccgctcga cgcgggacat gtgccgccgc cgcccgacct cgtcgacgcc
     2341 ctgatggacg acctgtgcga gttcgtctcg gcgccgtcgt cgacgccacc actgctgcgg
     2401 gccgcactgg ctcacgcgca gttcgagacc attcacccgt tcctcgacgg caacggacgc
     2461 accggacgcg ccctggtgca cctgatgctg cgccgcgacg ggctgctcga ccacacggtg
     2521 ttgccactgt cgggcacgtg ggggagggat aagcctcgct acatcgagta cctgaacgcg
     2581 ttgcgcacac agggcgatgg ttggggcacg gacacgctgg acgctgccgc acacttcatg
     2641 gccgactcga cgacggaggc cgccacgggg gcctacacga tcgccgctgc ggttcaaggc
     2701 gtggagaccg gcatggagaa gagtgtggcc gagaacttcc gtgccgactc ggtcgctcat
     2761 cgcgtcgtgc atgacgtgtg cgtgaacctg ggcgtcaccg tcgatggtgt cgcagcgcgt
     2821 catggcgctg acccggctgc ggtcctgcga gccctggacc ggatggaggg tctggaaatc
     2881 gtgaagcggc gctcagtgca gcggccccac gtgttctacg cgcccgagat ggtgcgtctg
     2941 gtcgaacggt tcgcggcgca gatccctgag ggagtggtcg aggggaaggg gacgccggac
     3001 accggggcgc tgctgacgat tgcgacggcg gaggggctgc tgacgtccgg cgagaccaag
     3061 ggtcgttgcg gtgagtggat gccccgcgct cgcgctacgt gcgctttgtc caaggggcac
     3121 cccgggtggc cgacgtcagg gcacaggcag ggtggggccg cttgagcacc cggacatgta
     3181 gccgtggata ccgttgagtc atgagcggct gcacggcacc acggttgggc cacggcacgt
     3241 ccggcgcccg gcagaagtgt ccggtgcacg gcaggccgat gcgtcccgca gatagggccg
     3301 cggccaagac ggcgttgggc agcatggcgc cgccgctgtg gcagaccgcg accgacccga
     3361 aggagtcgcc tgaggtgctg gcgattctgg ccgtcgaggg caagcaggac gtccgcgaac
     3421 gtgttgcctg caatccgcgc gcgtctcacg aaactctcga actgctggcg aaggaccccg
     3481 agaag
//
LOCUS       DASRKH010000459         2071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_65700, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000459 DASRKH010000000
VERSION     DASRKH010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65700"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            429..662
                     /locus_tag="VFC06_07385"
     CDS             429..662
                     /locus_tag="VFC06_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81737.1"
                     /translation="MPILTGRGLPPHGVLWPSLATTSAGYSVAVAGAIIVGAAWWAAI
                     FNADLLEDKTWFRRLRWTGVAAALTMPCSASGR"
     gene            complement(939..1313)
                     /locus_tag="VFC06_07390"
     CDS             complement(939..1313)
                     /locus_tag="VFC06_07390"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZL81738.1"
                     /translation="MSASDNKTATQAAYQAFANADLDGAMQDMADDIEWIVPGNSTIS
                     GTYRGKDEVRQLFVTLAGKSFRTEPEHFVADGDRVVVLTRVTADGQSSEQAAVLTFRE
                     GKIVKFQGGGDTAQQEKIWGSK"
     gene            complement(1545..1793)
                     /locus_tag="VFC06_07395"
     CDS             complement(1545..1793)
                     /locus_tag="VFC06_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81739.1"
                     /translation="MAKRLITVNDILAGHVSLDVACLDRVYLNGYLPTVQVPGQVVQF
                     LQHGGFPGARPGTTTHSFAIRSPPAGTAARRRLSPLTN"
BASE COUNT          325 a          688 c          693 g          365 t
ORIGIN      
        1 gcggacgcct ctaccgtgcg cgccgtcctt tcctcgcagg cggcggaaga tggtgtcctg
       61 ggcaacgacg gagcagacgg atgttgaact cgggcagagc agccccaggt tccgcgaagg
      121 cggtttgtgt cgccctacgc tttagtgcaa gcagttcccg gcggcacgtt caggtcagtc
      181 cgtgaccggg cgaacacggg acttttggcc ctggcgccga cggatacgcg agccgacggt
      241 cggcgccaaa gtggggcgtg ccaccaggag ataccgggag gtaggccatg tccatggcta
      301 agcggaagat cagtcagtgg tggtttgcgg gtcttgcggc gatgttcccg gcgggggttt
      361 tgatcttttg atcccatcca gcgcgctggc gctggccgcc cgacaggggg gcgatggtgt
      421 ccgcgacggt gcccatcctg accggtcggg gccttccgcc gcacggcgtc ctctggccat
      481 cgctggccac cacgtcagcg ggatactcag tggccgtcgc gggcgcgatc atcgtgggtg
      541 ccgcatggtg ggccgcgatc ttcaacgccg acctgcttga agacaagacc tggttccggc
      601 ggctgcgttg gaccggagtc gccgccgccc taaccatgcc ctgctcggct tcggggcgct
      661 gatcttgctc gtgtcgctct ctgcctatgt ccggcgggcg ccggacggac tgaccgtcgg
      721 cgcgctacag actcgcgtgc ccaccttgac acggacgtaa cgcgtgcagg gggagagcga
      781 acggcgcaca cggctgcccg gtcgatagcg ccgggacggc atcgaccgct gccgcgtctt
      841 tcggccgcga ccgcaactgg cgctaacgcg ggctcccgac cggccccggc gccaggtgca
      901 ataggggtcg ccgtcgtcag caacctcctg cgaggcggct acttgctgcc ccagatcttc
      961 tcctgctggg cagtgtcgcc tccgccctga aacttcacga tcttgccctc gcggaacgtc
     1021 agcacggcag cctgttccga cgattgaccg tcagcggtga cgcgcgtcag cacgacgaca
     1081 cggtcgccgt cggcgacaaa gtgctccggc tcggtccgga aggacttacc ggccaatgtg
     1141 acgaagagct gcctgacttc gtccttgccg cgataggtgc cgctgatcgt gctgttgccc
     1201 ggcacgatcc attcgatgtc gtcggccatg tcctgcatcg caccgtcgag gtcggcattc
     1261 gcaaacgctt ggtacgcggc ttgggtcgcg gtcttgttgt cggatgcgga catcggcatc
     1321 tccccgtgtt ggcgtcgttg acgcttccac cttccaccca accacgctgt ctgtgtcgcg
     1381 gcaacggtcc gcttggacca atcgctatca cgaagccgag acgcgcggcg tccgaggggg
     1441 cgcgtcaagc cgtcgtgacc ggcggtcagc gtcatcggct ccctcgcttc gatccactct
     1501 tcaagagtcg atcgggtcag atcccgcggc gagcacacgt gccgttagtt agttagagga
     1561 ctgagtcggc gtctcgcggc ggtgccggcc ggcggcgagc gaatggcaaa ggaatgggtg
     1621 gtggtgccgg ggcgagcacc cgggaatccg ccgtgctgca agaactgcac gacctgcccg
     1681 ggcacctgca ccgtgggcaa gtagccgttg agatagacgc ggtccaggca ggccacgtcc
     1741 aggctcacgt gcccggccag gatgtcgttc accgtgatca accgctttgc catggcgcac
     1801 ctccacagcc acctggccgt cgtgtaccac catgactccc cactcccggc gacgtcaccc
     1861 caccagctca gccactgaac tcgacggtcc gaggcggagc ctcgttagac agtcggctca
     1921 gacgtggcgg acggtggtga tgatcgggga gacccggcgc cgccagtcgc cggagcgcac
     1981 ccagcaccag gccactacat caggcaatgc caagagcaac tcggcgtggc ccgctggtgt
     2041 tggtagcgga gcacgtcgac ggctcctgac t
//
LOCUS       DASRKH010000460         2560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_61683, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000460 DASRKH010000000
VERSION     DASRKH010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61683"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="VFC06_07400"
     CDS             complement(<1..684)
                     /locus_tag="VFC06_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HZL81740.1"
                     /translation="MAIFLTAASKVIVQGMTGTEGIKHTTRMLTSGTKIVGGVNPRKA
                     GTTVDFEINGATVSIPVFGTVSEAMAATGADVSVLFVPPPFTKSAVVEAVDAGMPLAV
                     IITEGVPVKDTAEFFTYAQSKGTRLIGPNCPGLITPGQSNVGITPATITGPGKIGLVS
                     KSGTLTYQMLYELRDLGFSTAVGIGGDPIVGTTHIDCLEAFQNDPETVAADLADHDDR
                     DGLGVVLERL"
     gene            complement(697..1860)
                     /gene="sucC"
                     /locus_tag="VFC06_07405"
     CDS             complement(697..1860)
                     /gene="sucC"
                     /locus_tag="VFC06_07405"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HZL81741.1"
                     /translation="MDLFEYQARDMFEKHGVPVLPGIVATTPAEARAAAEKLGGTVVV
                     KAQVKVGGRGKAGGVKVVHNPADAEAAAEAILGMDIKGHTVHKVMIAGGAKIAQEFYF
                     SVLLDRSERQYLAMCSVEGGVEIEVLAVERPEALAKIAIDPIVGIDAAKAAEIVATAG
                     FSADLAGPVAAVIEKLWLVYRDEDATLVEVNPLVLTEGGEIIALDGKVTIDENAGFRH
                     DDHEALEDKEAADPLEAKAKALNLNYVKLDGAVGIIGNGAGLVMSTLDVVAYAGAHFG
                     GVKPANFLDIGGGASAAVMANGLDVILHDPQVNAVFVNVFGGITSCIEVANGIVGALE
                     TLGTTATKPLVIRLDGSHVEEGRAILAAANHPLVTIVETMDGAAAKAAELAVA"
     gene            complement(1972..>2560)
                     /locus_tag="VFC06_07410"
     CDS             complement(1972..>2560)
                     /locus_tag="VFC06_07410"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZL81742.1"
                     /translation="VGSEAGWDVVAEAGDGREALAVVDEHRPDVVLMDIRMPHMDGLE
                     ATREITAGASGAKVIVLTTFEVDEYVFEAMRAGASGFVLKRVPPAELIEAVRVVASGE
                     SMLFPASTRRVIERFATPTSVADMPDLTEREGDVLRLLVRGLSNGEIAAELFVSVETV
                     KSHVASILTKLGVRDRTQAVIVAYESGFVTPGAPE"
BASE COUNT          402 a          860 c          868 g          430 t
ORIGIN      
        1 gaggcgttcc agaacgaccc cgagaccgtc gcgatcgtca tgatcggcga gatcggcggc
       61 gacggtctcg gggtcgttct ggaacgcctc caggcagtcg atgtgggtgg tgccgacgat
      121 cgggtcgccg ccgatgccca cggcagtgga gaagccgagg tcacgcagct cgtagagcat
      181 ctggtaggtc agggtgccgg acttggacac caggccgatc ttgccggggc ccgtgatggt
      241 ggcgggcgtg atgcccacat tggactggcc tggggtgatg agtccggggc agttggggcc
      301 gatcaggcgc gtgcccttcg actgcgcata cgtgaagaac tccgcggtgt ccttgacggg
      361 gacgccttcg gtgatgatca cggcgagggg catgcccgcg tcgactgcct cgacgacggc
      421 cgacttggtg aacggcggcg gcacgaagag caccgacacg tcggcgcccg tcgcggccat
      481 cgcctctgag acggtgccga acaccgggat ggacacggtc gcgccgttga tctcgaagtc
      541 gaccgtggtg ccggccttgc gcgggttgac gccgccgaca atcttggtgc cggacgtgag
      601 catgcgcgtg gtgtgcttga tgccctccgt gccggtcatg ccctggacga tgactttgga
      661 tgctgcggtg aggaagattg ccatgtctgg gctcctttaa gcgacggcca gctcggcggc
      721 cttggccgcg gcgccgtcca tggtctcgac aatggtcacg agcgggtggt tggcggccgc
      781 caagatggcg cggccctcct ccacgtggct gccgtctagc cggatgacga gcggcttggt
      841 ggccgtggtg cccaacgtct cgagggcgcc gacgatgccg ttggccacct cgatgcagga
      901 ggtaatgccg ccgaacacgt tgacgaacac cgcgttcacc tgcgggtcgt ggaggatcac
      961 gtcgaggccg ttggccatca cggccgcgga cgcgccgccg ccgatgtcca ggaagttcgc
     1021 gggcttgacg ccgccgaagt gtgctcccgc gtaggccacg acgtcgagcg tcgacatgac
     1081 caggccggcg ccgttgccga tgatgccgac cgcgccgtcg agcttcacgt agttgaggtt
     1141 gagcgccttg gccttggcct cgagcgggtc agcggcttcc ttgtcctcga gcgcctcgtg
     1201 atcgtcgtgg cggaagccgg cgttctcgtc gatggtgacc ttgccgtcca gcgcgatgat
     1261 ctcgccgccc tcggtaagga ccagcgggtt gacctcgacc agggtggcgt cctcgtcgcg
     1321 gtaaaccagc cacagtttct cgatgacggc ggcgacgggt cccgccaggt ccgcagagaa
     1381 gccagcggtc gccacaatct cggcggcctt cgccgcgtcg atgccgacga tagggtcgat
     1441 cgcgatcttg gcgagcgcct cgggacgctc gaccgcgagt acctcgatct ccacgccgcc
     1501 ctcaacggaa cacatggcca gatactgacg ctcggagcga tccaacaaca cggagaagta
     1561 gaactcctgc gcgatcttgg ctcccccggc gatcatcacc ttgtgaaccg tgtggccctt
     1621 gatgtccatg ccgagaatgg cctcggcggc agcctcggcg tcggcggggt tgtgcaccac
     1681 cttgacgccg ccggccttgc cgcggcctcc caccttgacc tgcgccttga cgacgacagt
     1741 gccgcccagc ttctctgccg cggcgcgtgc ctcggctggg gtggtggcga cgatgcccgg
     1801 cagcacgggc acgccgtgct tctcaaacat gtcgcgcgct tggtattcga agaggtccat
     1861 cggattcctt gacgctttgg ggcgcgcggt cagcgcgacc atggggaaga tgcccccagc
     1921 ctagccgcgc tcgacgggcc ggaatgcgca cggtcgccca gacgggccca cctactcagg
     1981 agctcccggc gtcacgaacc ccgactcgta ggcgacgatc acggcctgcg tgcggtctcg
     2041 cacgcccagc ttggtgagga tcgacgccac gtgagacttg acggtctcta ccgagacgaa
     2101 cagttcggcc gcgatctcgc cgttggacag gccccgcacc aagaggcgta gcacgtcgcc
     2161 ctcgcgttcg gtgaggtcgg gcatgtcggc aacgctggtg ggcgtggcaa agcgttcgat
     2221 cacccggcgg gttgacgccg gaaacagcat cgactcgccc gacgcgacca cccgcaccgc
     2281 ctcgatcaac tcggcggggg gcacgcgctt cagcacaaac cccgaggccc cggctcgcat
     2341 cgcctcgaac acgtactcgt ccacctcaaa ggtggtgagc acgatcacct tcgcacctga
     2401 cgcccccgcg gtgatctccc gggttgcctc aagcccgtcc atgtggggca tgcggatgtc
     2461 catcagaacg acgtccggac gatgctcatc cacgacggcg agcgcctccc ggccgtcccc
     2521 cgcctcggcc acgacgtccc agccggcctc tgaaccgacg
//
LOCUS       DASRKH010000461         2124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_34338, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000461 DASRKH010000000
VERSION     DASRKH010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34338"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="VFC06_07415"
     CDS             <1..862
                     /locus_tag="VFC06_07415"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZL81743.1"
                     /translation="VHGLAVERWHGRSIINSLHASWSVGATVGGVIGATCAAFGVPIG
                     TQMVVGAVVWAVVAVVAGFEGAVPAEHLRRVESAADDGSAAADGAATATRRRFPWKLL
                     APLVVLAICGTLVEDVANNWVTLYLNRDVGAPIGLAGLGFAAMLGAQFVGRLLGDPMT
                     DRWGRAAVARSGGVLILLGSLTAVLAPHPLIAIAGFAIAGFGSATLVPAAYAAADNLP
                     GIAHGSGVAMLGWLMRFGFLLTSPMIGIIADGVGLRLAMLVPALAGLVAAVIAHLVGR
                     RERAGRPARA"
     gene            941..1471
                     /locus_tag="VFC06_07420"
     CDS             941..1471
                     /locus_tag="VFC06_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81744.1"
                     /translation="MHPLKKAWAAIAVLGGTAVVGSYVWGLSSIPNASTDFWGGTPEA
                     LIPLYTVSMLLGAVSFFFITAFVLVLDPATARVGEGFGFPVFLWIYLAILVPSALWMP
                     LVAQMVASPSEPVWWVIRLALLVVGLASVALVAALLRVSPRSPTWLYLVSVVGAVWFT
                     VHTLVLDALLWPVFFR"
     gene            1631..1876
                     /locus_tag="VFC06_07425"
     CDS             1631..1876
                     /locus_tag="VFC06_07425"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZL81745.1"
                     /translation="MGGTLRLVEAIKAAAHDPLLVYASSVTVLGYPSLPVLRTADDPV
                     GPSDHYTRHKIAAEERLRNGSARHAILRVGCRSMHAR"
     gene            1852..>2124
                     /locus_tag="VFC06_07430"
     CDS             1852..>2124
                     /locus_tag="VFC06_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81746.1"
                     /translation="MSIDARTLSTDLATLRQLLRTAPDNPIEYVHPLDVATAIANCLD
                     NPDAVGRVLLVGGGEGCRLTQHKFFSAVFEEAGLRLPREFMGDEAYY"
BASE COUNT          247 a          737 c          769 g          371 t
ORIGIN      
        1 cgtccacggg ctcgccgtcg agcggtggca cgggcgttcg atcatcaact cgctgcacgc
       61 gtcgtggagc gtcggcgcga ccgtcggcgg cgtcatcggc gccacctgtg ccgccttcgg
      121 cgtgccgatc gggacccaga tggtggtggg cgccgtcgtc tgggcggtgg tggccgtggt
      181 cgccgggttc gagggtgcgg tgccggccga gcacctgcgc cgtgtcgagt ccgcggcgga
      241 cgacggcagc gcggcggccg acggcgccgc gaccgcgacg cggcggcgct tcccgtggaa
      301 gttgctggcg ccgctcgtcg tgctcgcgat ctgcgggacc ctcgttgagg acgtggccaa
      361 caactgggtg acgctctacc tcaaccgcga cgtcggcgcc ccgatcggcc tggcgggcct
      421 cggtttcgcg gccatgctgg gcgcccagtt cgtgggccgg ctcctcggtg accccatgac
      481 cgatcggtgg ggccgggccg ccgtggcgcg cagtggggga gtgttgatcc tgctcggctc
      541 gctcacggcg gtgctcgcac cgcacccgct catcgcgatc gccggcttcg cgatcgccgg
      601 gttcggctcg gccacgctcg tgcccgccgc ctacgccgcc gccgacaacc tccccggcat
      661 cgcccacggc tccggcgtcg cgatgctcgg ctggctcatg cgcttcggct tcctactcac
      721 ctcgcccatg atcggcatca tcgccgacgg cgtcgggctc cgcctcgcca tgctggtccc
      781 ggcgcttgcc ggactggtgg cggcggtcat cgcgcacctg gtcggccggc gcgagcgtgc
      841 gggccggccc gcacgcgcct gagggatgct cgcccgccct gcatccagaa tggccgtggg
      901 ttcggaagta cgttatgtga gcgaaaacca agaagggcgc atgcatccac tgaagaaggc
      961 ctgggccgcg atcgcggtcc tgggcggcac ggccgtggtt ggcagctacg tgtgggggct
     1021 gagctccatc ccgaacgcga gcaccgactt ctggggtggg actccggagg ccctcatccc
     1081 gctgtacacg gtgagcatgc tcctcggggc ggtgagcttc ttcttcatca ccgcgttcgt
     1141 gctcgtcctg gaccccgcca ccgcccgcgt gggggagggg ttcgggttcc cggtattcct
     1201 gtggatctac ctcgccatcc tggtcccgtc cgcgctgtgg atgcccttgg tggcgcagat
     1261 ggtcgcctcc ccgagcgagc cggtgtggtg ggtcatccgg ctcgcactgc tggtggtggg
     1321 cctcgcctcg gttgccctcg ttgccgcact gctgcgggtg tcgccgcgct cgcccacgtg
     1381 gctgtacctc gtctccgtgg tgggtgccgt ctggttcacg gtgcacaccc tagtgctgga
     1441 cgccctcctc tggccggtgt tcttccggtg acccgggtcc tggtcaccgg ggccttcgga
     1501 cggctcgggc aggtcgtgct ccgcgagctg aagcattcgt cgacgacgtc gtctggggcg
     1561 acgttcgcca cgcggacctg gggccgcttg tggaccggag acggcaccgg acctggcgga
     1621 gtcggtcaac gtgggcggca ccctgcgctt ggtggaggcc atcaaagcgg cggcgcacga
     1681 cccgttgctg gtatacgcgt cgtctgtgac ggtgttgggg tacccgtcgc ttccggtcct
     1741 gcggacagcg gatgaccctg tggggccgtc ggaccactac acacggcaca agatcgcggc
     1801 ggaggagcgg ctgcggaatg gcagtgcgcg ccacgcgatc ctgcgtgtgg ggtgtcgatc
     1861 gatgcacgca cgctgagcac ggacctcgcc acgctgcggc aactgctgcg gaccgccccc
     1921 gacaacccca tcgagtacgt ccaccccctc gacgtcgcca ccgcgatcgc caactgcttg
     1981 gacaacccgg acgccgtcgg aagggtgctg ctggtggggg gcggggaggg ctgccgcctc
     2041 acccagcaca agttcttcag cgccgtattc gaggaggccg gcctgcgcct gccacgcgag
     2101 ttcatggggg acgaggcgta ttac
//
LOCUS       DASRKH010000462         2102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_58939, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000462 DASRKH010000000
VERSION     DASRKH010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58939"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="VFC06_07435"
     CDS             complement(<1..667)
                     /locus_tag="VFC06_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HZL81747.1"
                     /translation="MLSRIAESLFWIGRYVERADNTARLLDVHLNVLLEDPWVDEPTA
                     NASLLAVMHVPVEGTATREAVLQALATDATLSSSITGALGAARDNARRAREVISTEVW
                     ESLNTTYMQLGARSRNPRPHDYFNWVRERAAVVWGLHSATTSRDDAWNFMELGRSLER
                     ADMIARLLMTKALAGSAGPNWTTLLRTCSAHEAYLRTVRSLVTEERAAEFLLIDRQFP
                     RSIA"
     gene            complement(661..>2102)
                     /locus_tag="VFC06_07440"
     CDS             complement(661..>2102)
                     /locus_tag="VFC06_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HZL81748.1"
                     /translation="SQMFPDLFGAMGVRPVLEYSQRLRSALAKAAPEGVEDPSIVVLT
                     PGVAQRVRTLEAFLSDVYGSQRCVTDGIIPRRLITTSQYFHRVVGAVDPANGVRVHVS
                     GIDLVRDEHGIWRVLEDNVRVPSGVSYVLSNRRAMSQMFPDLFGAMGVRPVLEYSQRL
                     RSALAKAAPEGVEDPSIVVLTPGVFNSAYYEHSLLARTMGVELVEGRDLETHAGRVYM
                     RTTAGRRRVDVIYRRVDDEFLDPVVFRSDSALGAPGIIHAARLGNVTIANAIGNGVAD
                     DKLIYTYMPELTRYYLGEDPILPNVDTWRLEDPESLAEVMDRLDELVTKPVDGAGGKG
                     VLIGPAASKSELEIARNHILEDPRGWIAQPVVQLSTVPTLTNDGLEPRHVDLRPFAIN
                     DGDDVWVLPGGLTRVALQRGQLIVNSSQGGGSKDTWVLGGVRHRGSVPTPESRVEFSG
                     AAPVPQSAHPEDYVHGQQQMQQQQQGSTC"
BASE COUNT          340 a          706 c          724 g          332 t
ORIGIN      
        1 aggcgataga gcgcgggaac tgccggtcga tcagcaggaa ctccgcggcg cgctcctccg
       61 tcacgagcga cctcacggtg cgcagatacg cctcgtgcgc ggagcacgtg cgcagcaggg
      121 tggtccagtt gggtcccgcg ctgcccgcga gtgccttcgt catgagcagc cgcgcgatca
      181 tgtcggccct ctcgagcgag cggccgagtt ccatgaagtt ccacgcgtcg tcgcgagacg
      241 tggtcgcgct gtgcaggccc cacaccacgg cggcccgctc ccgcacccaa ttgaagtagt
      301 cgtgcgggcg cgggttgcgc gagcgcgcgc ccaattgcat atacgtcgtg ttcagggact
      361 cccacacctc ggtggaaatg acctcgcggg cgcggcgggc gttgtctcgg gcggcgccca
      421 gggcgcccgt gatcgaggac gacaaggtgg cgtcggtcgc aagcgcctgg aggaccgctt
      481 cgcgggtcgc cgtgccctcg acggggacat gcatcactgc gagaagcgac gcattggcgg
      541 tgggctcatc cacccaggga tcctcaagca acacgttgag gtgcacatca agcaagcggg
      601 ccgtgttgtc cgcgcgctcc acatagcgcc cgatccagaa gagcgactcg gcgattcgac
      661 ttaacatgtc gacccctgct gttgctgttg catctgctgc tgtccgtgga cgtagtcctc
      721 cgggtgggcg gactggggca ccggcgccgc ccccgagaac tcgacgcgcg actcgggcgt
      781 gggcacggag ccgcggtgac gcacgcctcc aagcacccac gtgtccttgg agccgccgcc
      841 ttgagaagag ttcacgatca actggcccct ttgcagggcc acacgggtga ggccgccggg
      901 caggacccac acatcgtctc cgtcgttgat ggcgaagggc ctgaggtcaa cgtggcgggg
      961 ctcgaggccg tcgttcgtga gcgtcggcac cgtcgagagt tgcaccacgg gttgcgcgat
     1021 ccacccgcgt ggatcctcga ggatgtggtt gcgggcgatc tcgagttcgc tcttgctcgc
     1081 cgccgggccg atcagcacgc ccttgccacc ggcgccgtcg acgggcttgg tcacgagctc
     1141 gtccagccta tccatgacct ccgcgagcga ctcggggtcc tcgagccgcc aggtgtcgac
     1201 gttcggcagg atcgggtcct cgcccaggta gtagcgggtg agttcgggca tgtacgtgta
     1261 gatgagcttg tcgtcggcga cgccgttgcc gatcgcgttg gcgatggtga cgttgcccaa
     1321 ccgagccgcg tgaatgattc ccggcgcacc cagggcggag tcggaacgga acacgaccgg
     1381 gtccaggaac tcgtcgtcga cgcggcgata gatgacgtcc accctgcgcc tgccagccgt
     1441 cgtccgcatg tagacgcgac ccgcgtgagt ctcgaggtcg cgcccctcca ccagctcgac
     1501 gcccatggtg cgcgccagga gcgagtgctc gtagtacgcc gagttgaaga cgccgggcgt
     1561 cagcaccacg atcgacgggt cctcgacgcc ttcgggcgcg gccttcgcga gcgcggaccg
     1621 caggcgctga ctgtattcga gcaccgggcg cacgcccatg gctccgaata ggtcggggaa
     1681 catctggctc atagcgcggc ggttggacaa cacgtagctc acgccggagg gcacgcgcac
     1741 gttgtcctcc agcacccgcc agataccgtg ttcgtcgcgc accaggtcga tgccggagac
     1801 gtggacgcgc acaccattcg cggggtccac cgcgcccacc acacggtgga agtactgcga
     1861 cgtagtgatg aggcggcgcg ggatgatgcc gtccgtgacg caccgctgcg aaccgtagac
     1921 atccgacagg aacgcctcga gggtgcgcac ccgctgcgcc acgccgggcg tgagcaccac
     1981 gatcgacggg tcctcgacgc cttcaggcgc ggcctttgcg agcgcggacc gcagccgctg
     2041 gctgtactcg agcaccgggc gcacgcccat ggcgccgaag aggtccggga acatttggct
     2101 ca
//
LOCUS       DASRKH010000463         2558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_52648, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000463 DASRKH010000000
VERSION     DASRKH010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52648"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..463
                     /locus_tag="VFC06_07445"
     CDS             167..463
                     /locus_tag="VFC06_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81749.1"
                     /translation="MASADDDLESLSRGELVQEVLRLRNGIRQHRDSSGHDLCWYHPQ
                     LWSLLPHESDPLPAVPEWPEFMRGCVGYRQSLDDQLAVAPRVHIEYDPGPSEQA"
     gene            complement(504..1091)
                     /locus_tag="VFC06_07450"
     CDS             complement(504..1091)
                     /locus_tag="VFC06_07450"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HZL81750.1"
                     /translation="MNDQAHWADELDRAVRVAVVAMSGADSADWSLPAGSLEWDCRTT
                     VCHVASDFVGYAGQLTAPREHGYAPFDVVLEGVPDPTGLCDMVRATGGVLSAVVRTAP
                     AETLGWHPYGMAGPQDFAAMGMVELLVHTEDLSRGLGFPWNPPDDLCERVLAYLFPGA
                     AVDHEPWPTLLWATGRVALEALPRQDSWRWTNAGT"
     gene            1233..1673
                     /locus_tag="VFC06_07455"
     CDS             1233..1673
                     /locus_tag="VFC06_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81751.1"
                     /translation="MGAAMTFDEKNTWIYGVVALGAYGVYLVTVLARAHGGPVVEVAY
                     IAPMLWCIGAAIVAAILGKVAVSARWPREANHRDDRDREIDRFGEHVGQSFLVIGLVA
                     ALVLAMAEIDHFWIANAAYLAFVLSAALGSGAKIAAYRGGFQPW"
     gene            1667..1900
                     /locus_tag="VFC06_07460"
     CDS             1667..1900
                     /locus_tag="VFC06_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZL81752.1"
                     /translation="MVKPTKITNSIRALRFANGEMTQAELARRIGVTRQTVIAIEQGR
                     YSPSLEMAFQIARAFGVPLDAVFLYPDDSEEHS"
     gene            1897..>2558
                     /locus_tag="VFC06_07465"
     CDS             1897..>2558
                     /locus_tag="VFC06_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018774819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HZL81753.1"
                     /translation="MKAMVQDTYGSTDVLRLDDVDPPEVGPEDVLVRVRAAGVDAGVW
                     HLMAGQPYLVRVMGFGLRAPKARTRGMEVAGVVEAVGAQVTQLRPGDPVFGTCDGSFA
                     EYATARPSSLARMPSTIDFEQAAAVPVSGSTALQALRDAGKVEPGQRVLVIGAGGGVG
                     TFAVQLAKAFGAEVTGVCGTAKVDLVRSLGADDVVDYTSEEITAAGRRYDLVLDTAGN
                     RPL"
BASE COUNT          388 a          900 c          883 g          387 t
ORIGIN      
        1 cgtgaacacc gctccgatga cgacgagcat cgcgccgagt gcgatgacgg ctgccttgct
       61 caccggttcc tcctgcggat ggcgacgcgc agctcgatca ggctgcggat ccagtgtcat
      121 gccgctcgca ggtgcgcgcc acacgccggg cgcttgcagt cgcgcaatgg cctcggcaga
      181 cgacgacctg gagtcactct cacgcggcga gctcgtccag gaggtgctgc ggcttcgcaa
      241 cggcattcgg cagcatcggg acagcagcgg tcacgacctg tgctggtacc acccacagct
      301 ctggagtctg ttgccgcacg agtcggaccc tcttcccgcc gtgccggagt ggccggagtt
      361 catgcgcggc tgcgtcggct atcgccagtc cctcgacgac cagctcgccg tcgcgccccg
      421 ggtgcacatc gagtacgacc ccgggccgtc cgagcaggcc tgagggcggg gcccgagcgg
      481 aggagcggtg atctaccccc ggcttacgtc ccggcgttcg tccaccgcca ggagtcctga
      541 cgcggcagcg cctccagggc gacgcgtccg gtagcccaca ggagcgtggg ccacggctcg
      601 tggtcgacag cggcgcccgg gaacaggtat gccaagacgc gctcgcacag gtcgtccggc
      661 gggttccacg ggaagccgag accgcggctg aggtcctcgg tgtgcacgag gagctcgacc
      721 attcccatgg ccgcgaagtc ctggggtccc gccatgccgt acgggtgcca gccgagcgtc
      781 tccgcaggtg cggttcggac gacggcgctg agcacgccgc ccgtcgcccg caccatgtcg
      841 cacagtccag tggggtcggg tactccctcg aggaccacgt cgaacggcgc gtagccgtgc
      901 tcccgaggtg cggtcagctg cccggcatac ccgacgaagt cgctagccac atggcagacc
      961 gtggtacggc agtcccactc cagagagccc gccggcagag accagtcggc tgagtccgcg
     1021 ccggacatcg cgacgaccgc gacgcgtaca gccctgtcca gctcgtcggc ccagtgcgcc
     1081 tggtcgttca cccgcgtgcc tgttcccgga cctcacgcat catgcggccg atggtgccac
     1141 gcatcggcac agcgttcgac cgatcgagat gtcaaacatg attgacatca tgtcggccat
     1201 gccatacgtt caccatgtca aacatttctg acatgggagc agcgatgacg ttcgacgaga
     1261 agaacacgtg gatctacggc gtcgtcgcgc tcggcgcgta cggggtctac ctggtgacag
     1321 tgctcgcacg ggcccacggg ggacccgtcg tcgaggtcgc ctacatcgcg ccgatgctgt
     1381 ggtgcatcgg tgcggcgatc gtggcggcga tcctcggcaa ggtcgcggtc tccgcgcgct
     1441 ggccgcgcga ggccaaccac cgggacgacc gcgaccggga gatcgaccgg ttcggcgagc
     1501 acgtcggcca gtcgttcctg gtcatcggtc tggtggcggc gctcgtgctg gcgatggcag
     1561 agatcgacca cttctggatc gccaacgcgg cctacctcgc gttcgtcctg tcggcggccc
     1621 tggggtccgg cgccaagatc gcggcctacc gcgggggctt ccagccgtgg tgaagccgac
     1681 caagatcacc aactcgatcc gggccctacg gttcgcgaac ggggagatga cgcaggccga
     1741 gctggcccgg cgcatcggtg tcacccgcca gaccgtcatc gccatcgagc aggggcggta
     1801 ctcaccgtca ctcgagatgg cctttcagat cgcgcgggcg ttcggcgtcc cgctcgacgc
     1861 ggtcttcctg taccccgacg actctgagga gcactcatga aggccatggt ccaggacacc
     1921 tacggttcca ccgacgtcct ccgcctggac gacgtcgacc cgccggaggt gggccccgag
     1981 gacgtgctcg tgcgggtacg cgccgcaggc gtcgacgccg gcgtctggca cctgatggcg
     2041 ggccagccct acctggtgcg tgtgatgggc ttcggcctgc gcgctcccaa ggcgcggacg
     2101 cggggcatgg aggtcgccgg ggtcgtcgaa gccgtcggcg cccaggtgac gcagctgcgc
     2161 cccggtgacc ccgtgttcgg aacctgcgac ggctcgttcg ccgagtacgc gacggcccgc
     2221 cccagcagcc tggcccgcat gccgagcacg atcgacttcg agcaggcggc cgctgtcccc
     2281 gtctcgggca gcaccgctct gcaggccctg cgcgacgcgg gaaaggtcga acccgggcag
     2341 cgggtcctcg tcatcggtgc agggggaggc gtggggacgt tcgccgtcca gctcgccaag
     2401 gcgttcggcg ccgaggtcac cggcgtgtgc ggcacagcca aggtggacct ggtccgctcg
     2461 ctcggggccg acgacgtcgt cgactacacg agcgaggaga tcaccgccgc gggacgccgc
     2521 tacgacctcg tcctcgacac cgcgggcaac cgtccgct
//
LOCUS       DASRKH010000464         2169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_16416, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000464 DASRKH010000000
VERSION     DASRKH010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16416"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="VFC06_07470"
     CDS             <1..554
                     /locus_tag="VFC06_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HZL81754.1"
                     /translation="AHAASASAALVAKGAALRTAGATIHVIGQLQRNKASVAVQWADC
                     VQTVDSLRLAERLSRLCVEAERKLDVMIQVNVSGESSKAGVDPEEALALAAAVQNLPA
                     LAVIGFMTIGLNSPDESAVRAGYARLRALRDEALVKSESGGLPLKDAWELSMGMSGDL
                     EWAIAEGATIVRVGTAIMGHRRD"
     gene            624..1349
                     /locus_tag="VFC06_07475"
     CDS             624..1349
                     /locus_tag="VFC06_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018774506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HZL81755.1"
                     /translation="MFKSTHKADLFRGPDEADISSRLNWLRAGVLGANDGIVSIAALL
                     VGVAAATTDANVIFTAAMAGIAAGALSMGVGEYVSVSSQRDAEDAQLARERIWQKARP
                     AWELEQLVRLNMETGMSEETARRAATEQHDHDPLTIHAKMHLGIDPDELTNPLAAGVA
                     SLIAFTIGGIAPLLTILLLPDHLRIPLTFAAVLVALAGTGYTSARVGYAPITRAVLRN
                     MAGGMTAMVITYGIGLLVGTAVG"
     gene            complement(1346..>2169)
                     /locus_tag="VFC06_07480"
     CDS             complement(1346..>2169)
                     /locus_tag="VFC06_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HZL81756.1"
                     /translation="FSPEAVAIAVRRALGHATSWHDHTFDVIHDVPREPHLQMALDDV
                     LAQEVGAGERGPALRIWEWERNTVVIGSFQSVRNEIDPDGAAAHDITVTRRVSGGGAM
                     FIQPGNTITYSLYVPTSLVEGLSFEQSYAFLDDWVLGALRELGIQATYVPINDIASPA
                     GKIAGAAQKRYLSGAVLHHVTMAYDIDADAMLEVLRIGREKMSDKGTKSANKRVDPLR
                     SQTGMAREAIIESFLGHFASRYATIPGVVSDVEVAKAHALVTEKFSTPEWIHVVP"
BASE COUNT          362 a          722 c          730 g          355 t
ORIGIN      
        1 cagcacacgc ggcgtcggcg tccgcggcct tggtcgccaa gggcgcggca ctgaggactg
       61 cgggcgcgac gattcacgtg atcgggcaac tgcagcgcaa caaggcgtcg gtggcggtgc
      121 agtgggctga ctgcgtgcag accgtcgact cgctccggct ggccgagcga ctgtcacggc
      181 tgtgtgtcga ggcggagcga aagttggacg tgatgattca ggtcaacgtg tctggcgagt
      241 ccagcaaggc gggagtcgat ccggaggagg ccctcgcgct ggctgcggcc gtccagaacc
      301 tgcccgcgct tgccgtgatc gggttcatga cgatcggcct caactcaccc gacgagtccg
      361 cagtgcgtgc cggctatgcg cggctccgtg cactgcggga cgaagcgctc gtgaagtccg
      421 aaagcggcgg actgccactc aaggacgcgt gggagttgtc gatgggcatg tccggcgacc
      481 tcgagtgggc gatcgcggag ggcgcgacga tcgtgcgcgt gggcaccgcc atcatggggc
      541 atcgtcgcga ctgagcggca cccgccaccc gagcaaggac gcccttgcta gatgggcgcg
      601 acgtgcggca gtaatctttc cccatgttca aatccacgca caaagcagac ctgttccgcg
      661 gccccgacga ggcagacatc tcgtcgcgtc tgaactggct gcgtgcggga gtgctgggcg
      721 cgaacgacgg aatcgtgtcg atcgccgccc tgctggtggg cgtcgcggcc gcgacgaccg
      781 acgcgaacgt gatcttcacg gccgcgatgg ccggcatcgc ggcgggcgcc ttgtcgatgg
      841 gcgtcggcga gtacgtgtcg gtatccagcc aacgcgacgc agaagacgcg caactggcga
      901 gggaacgcat ctggcagaag gcccgccccg catgggaact cgagcagctg gtgcgactca
      961 acatggaaac cgggatgtcg gaggagacgg cccggcgggc tgccacggag cagcacgacc
     1021 acgatccgct caccattcac gccaagatgc acctcggcat cgacccagac gagctcacga
     1081 atccgctcgc ggcgggggtc gcctctctga tcgcgttcac catcggcggc atcgcgccgc
     1141 tgctcaccat cctgctgctc cccgaccacc tgcgcatccc gctcaccttt gcggcggtac
     1201 tggtggctct ggcgggcacc ggctacacgt ccgctcgggt cgggtatgcg ccgatcacgc
     1261 gcgccgtgct ccgcaatatg gccggtggca tgaccgccat ggtgatcacc tacggcatcg
     1321 gcctgttggt tggtacggcc gtcggctagg gaacgacgtg gatccactcg ggcgtggaga
     1381 acttctcggt cacgagtgcg tgggccttcg cgacctccac gtcgctcacg acaccgggta
     1441 tcgtcgcgta gcgcgacgcg aagtggccca ggaacgactc gatgatcgcc tcgcgcgcca
     1501 tgcccgtctg gctgcgcaac gggtccacgc gcttgttcgc gctcttggtg cccttgtccg
     1561 acatcttctc gcggccgatg cgcaacacct caagcatcgc gtctgcgtcg atgtcgtagg
     1621 ccatggtgac gtggtggagc accgctccag acaggtaacg cttctgcgcg gctcccgcga
     1681 tcttgcctgc tggcgaggcg atgtcgttga tcggcacgta cgtggcctga atccccagct
     1741 cccgcaacgc cccgagcacc cagtcatcca ggaacgcgta cgactgctcg aagctcagcc
     1801 cctccacgag cgacgtgggc acgtagagcg agtaggtgat ggtgttaccc ggctggatga
     1861 acatcgctcc gccaccagaa acgcgacgcg tcacggtgat atcgtgcgcc gccgcaccat
     1921 cggggtcgat ttcgttgcgc actgactgga aggagccgat caccaccgtg ttgcgctccc
     1981 actcccagat gcgcagcgcc gggcctcgct cgccggcgcc gacctcctgg gcgagaacat
     2041 cgtcaagcgc catctgcagg tgaggctcgc gcggaacgtc atggatcacg tcgaacgtgt
     2101 gatcgtgcca actggtggcg tggcccagcg cacgacgcac cgcgatcgcc accgcctccg
     2161 ggctgaagc
//
LOCUS       DASRKH010000465         2018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_15913, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000465 DASRKH010000000
VERSION     DASRKH010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15913"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="VFC06_07485"
     CDS             complement(<1..573)
                     /locus_tag="VFC06_07485"
                     /inference="COORDINATES: protein motif:HMM:NF014898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HZL81757.1"
                     /translation="MLDEFEAWLLRERSAQERTAAAYTARVAAFVEWLPAPVDQSLRK
                     LTAATVIEWVNLEAARGLKASTLGKQLVMLRSFLQFCHRSARTGQGLSGVVPHAAAWR
                     LSSIPDPVPAGTIEVLFDTLDLRSAKGLRDRAILLLLTGLGLRACEIADLRLDDIGWR
                     TGSLRIRGKGDRVDELPLPDEIGQALEDYVL"
     gene            886..1698
                     /locus_tag="VFC06_07490"
     CDS             886..1698
                     /locus_tag="VFC06_07490"
                     /inference="COORDINATES: protein motif:HMM:NF016114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HZL81758.1"
                     /translation="MVQISIQSETRGASSAARKLRDLYGIHAHVVPVRSGIGDAERLE
                     KVASVAAHLLGDWMQDDTVLGVAWGTTTWEVARQLTRHPTHGSMIVQLNGAANTHTTG
                     IGYASQIISTIAEAFDAKTLFFPVPAFFDYAETKAAMWRERSVSRVLGAQRRAQIVLF
                     GVGAMGGSTPSHVYAAGYLDDADMRAVAEEGVVGDICTVFLRADGTYRDIAINRRATG
                     PTPSELTRVARRVCVAAGDSKVSALIAALRAHVVTDLVVDEATARAVITAST"
     gene            complement(1816..>2018)
                     /locus_tag="VFC06_07495"
     CDS             complement(1816..>2018)
                     /locus_tag="VFC06_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HZL81759.1"
                     /translation="WDPLEAQPHDPDVKALLRIGAVWNIPMASNMATADFLISSPLFA
                     EHYDRAVPDLGDRDWATLRESH"
BASE COUNT          375 a          704 c          629 g          310 t
ORIGIN      
        1 gagcacatag tcctccaacg cctggccgat ctcgtcgggc agcgggagct catcgacgcg
       61 gtcgcccttg cctcggatac gcaggcttcc ggtgcgccac ccgatgtcat ccagccgcaa
      121 gtcggcgatc tcacacgcgc gcaaccccaa cccggtcaac aacagcagga tcgcccgatc
      181 acgcagccct ttcgccgaac gcagatcaag cgtgtcgaac agtacctcga tcgtgcccgc
      241 cggcaccggg tccgggatcg acgagagccg ccacgccgct gcatgcggca ccaccccaga
      301 caagccctgt cccgtccgag cagagcggtg gcaaaactgc aggaacgagc gcaacatcac
      361 cagctgcttg cccagcgtcg atgccttcaa cccgcgcgcg gcctccagat tcacccactc
      421 gatcaccgtc gccgccgtca gcttccgcag cgactgatcg accggcgcgg gcaaccactc
      481 cacgaaggct gcgacacggg cggtgtatgc agcagcggtc cgctcctgtg ccgaccgctc
      541 ccggagcagc cacgcctcga actcgtccag cacgacacgt gcgtccgcgc ggcgctgatc
      601 acgatgaatc accatctcgg tcctcctcga cctgacgcca agcgatccct gtgaccgccc
      661 agtcagatca agcgaacccc acttcggccg ccaatggtta tgttgaccgg atcagccgcc
      721 cagccccgca cgaccaggga cgccaccaga ccggtcaaca taaccacgag ggcgacatac
      781 catcgcccga tccctcctcg aatccggagg cttcagacca ctcctacacc cctaaatgcg
      841 aagagcccga tatcacgcac tttgaagaac gcgcgagaaa ccggcatggt gcagatctcc
      901 atccagagcg agacccgcgg cgcttcctcg gccgcgcgca agctccgcga tctctacggg
      961 atacacgcgc acgtagtccc ggttcgttcc ggtatcggtg acgccgagcg gctcgaaaag
     1021 gtcgcgagcg tcgcggcgca cctgctcggc gactggatgc aagacgacac ggtgctgggt
     1081 gtggcgtggg gcaccactac ctgggaggtc gctcgtcaac tcacgcgcca ccccacccac
     1141 ggcagcatga ttgttcaact caacggggcc gccaacacgc acaccacggg aatcggctat
     1201 gcgagccaaa tcatcagcac catcgctgag gcattcgacg ccaaaaccct cttcttcccc
     1261 gtgccggcgt tcttcgacta cgccgagacg aaagcggcga tgtggcgtga acgcagcgtg
     1321 tcccgcgtgc tcggggcgca gcgccgtgcg cagatcgtgt tgttcggggt gggggccatg
     1381 gggggtagca cgccgtcgca tgtgtacgcg gcgggatacc tggatgacgc tgacatgcgg
     1441 gcggtggccg aggagggggt cgtcggcgac atctgcacgg tatttctgag ggccgacggc
     1501 acgtatcgtg acattgcgat caaccgtcgg gccacgggtc ccacgccgtc cgagttgacg
     1561 cgggtggcgc ggcgcgtctg cgttgcggcg ggggacagca aggtttcggc gttgatcgcc
     1621 gctctccgcg cgcatgtggt caccgacctt gtcgttgacg aggcgaccgc gcgcgccgtc
     1681 atcacggcat cgacgtgacg tggtgcggtg agtccttcgc gcggcgcgcc tcatgccggc
     1741 gcagcccacg accgatcgag ccgatctgaa cgcgaccgga ccaacgtgcg gtgcgtgtga
     1801 gagggcgcag gaacgtcagt gtgactcgcg gagcgtcgcc cagtcgcggt cacccaaatc
     1861 gggcacggca cggtcgtaat gctccgcaaa caggggcgac gagatcagga agtcggccgt
     1921 tgccatgttc gacgccatgg gaatgttcca caccgccccg atccgtagca gtgccttgac
     1981 gtcggggtcg tgcggctgtg cctcgagcgg gtcccaga
//
LOCUS       DASRKH010000466         2455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_52708, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000466 DASRKH010000000
VERSION     DASRKH010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52708"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..1900
                     /locus_tag="VFC06_07500"
     CDS             179..1900
                     /locus_tag="VFC06_07500"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HZL81760.1"
                     /translation="MERSRFESRADHVTRHIFVTGGVVSSLGKGLTASSLGRLLRSRG
                     IRVTMQKLDPYLNVDPGTMNPFQHGEVYVTEDGAETDLDIGHYERFLDVKLSGDANVT
                     TGQVYSQVIAKERRGEYLGDTVQVIPHITDEIKRRMRAQAGPDVDVIITEIGGTVGDI
                     ESQPFLESARQVRHELGRDNVFFLHVSLVPYIGPSGELKTKPTQHSVAALRSIGIQPD
                     AIVCRADRDIPASVKNKIALMCDVEPQAVVTAKDAPSIYDIPRVLHSEGLDAYVVRRL
                     DLPFHDVQWGAWDDVLRRVHHPADEVEVALVGKYIDLPDAYLSVCEALRAGGFHHNAR
                     VNIRWVASDTCQTREGADKALGGVDAVLIPGGFGVRGVDGKIGAARWARENLVPTLGI
                     CLGLQVMVMEYARTILGLEGASSSEFDPETPHPVIATMEEQLEFVEGAGDLGGTMRLG
                     EYRAVLTPGSVVEQTYGAATVGERHRHRYEVNNFYRPQLEEAGLVVSGESPDRQLVEF
                     IELPRTAHPYYVATQAHPEFLSRPTKAHPLFAGLIGAALERQKDASATEQAPAAQDVA
                     PAASSAS"
     gene            1897..>2455
                     /locus_tag="VFC06_07505"
     CDS             1897..>2455
                     /locus_tag="VFC06_07505"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZL81761.1"
                     /translation="MTDEPRGELADVAAPRPTLARHERFQGRVWNLVSDDVELGHSQV
                     TSDFVQHTGAVAIVALNDSDEMYLVRQYRHPVGVECWEPPAGLLDVGDELPVEAAKRE
                     LHEEADLTAATWHVLADYFPSGGGSSEAVRVFLARDLAAVPEAERHVRTDEEHDMVGA
                     WVPLDEILAAIAAGRVHASSLVVGAM"
BASE COUNT          405 a          808 c          824 g          418 t
ORIGIN      
        1 gagtgttgtc atcgcgacgg aactggaact catgccgccg caactggtgt ttgcactggt
       61 agaacaggaa tccggaggcg cgggctcgta cggcacggcc gaaaatctgc ccctgattgc
      121 gtcgccatag cgtgcgagcc aactccagaa gccgctatca ctgttagtgt tgaagtccgt
      181 ggagagatct cgttttgagt cccgtgcgga ccatgtcacc cggcacattt ttgttacagg
      241 tggcgtggtg tcgtccctgg gaaagggcct gacggcttcc agcctcggcc gattgcttcg
      301 ctctcgcggt attcgcgtca cgatgcagaa gcttgacccg tacctcaacg tggatccagg
      361 gacgatgaac ccgttccagc acggtgaggt gtacgtgacg gaggacggcg cggagaccga
      421 cctcgacatc ggccactacg agcgcttcct cgacgtcaag ctctcgggcg acgcgaacgt
      481 caccaccggc caggtgtact cgcaggtcat cgccaaggag cgtcgcggcg aatacctcgg
      541 cgacacggtg caggtcatcc cgcacatcac tgacgagatc aagcgccgca tgcgcgctca
      601 agcgggcccc gacgtcgacg tgatcatcac cgagatcggt ggcacggtgg gcgacatcga
      661 gagccagccg ttcctcgagt cggcgcgcca ggtgcggcac gaactcggcc gcgacaacgt
      721 cttcttcctg cacgtctcgc tcgtgccgta catcggcccc tccggcgaac tcaagaccaa
      781 gcccacccag cactccgtag cggcgctgcg cagcatcggc attcagccgg acgccatcgt
      841 gtgccgcgcg gaccgcgaca tccccgcgtc ggtcaagaac aagatcgcgc tgatgtgcga
      901 cgtcgagccg caagccgtgg tgaccgccaa ggacgcgccg agcatctatg acattcccag
      961 ggttctgcat tccgagggac tcgacgcgta cgtggttcgc cgcctcgacc tgccgttcca
     1021 cgacgtgcag tggggggcgt gggacgatgt cctgcgtcgc gtccaccacc cggcggacga
     1081 ggtcgaggtc gcgctggttg gcaagtacat cgatcttccc gacgcgtacc tgtcggtctg
     1141 cgaggcgctg cgcgcgggtg gcttccacca caatgcgcgg gtcaacatcc gttgggtcgc
     1201 gtcggacacc tgccagactc gtgagggcgc cgacaaggcg ctcggcggcg tcgatgcggt
     1261 cctgattccg ggtggcttcg gcgtgcgcgg cgtggacggc aagattggcg cggcgcgctg
     1321 ggcgcgcgag aacttggtcc ccacgcttgg catctgcctc ggccttcagg tcatggtgat
     1381 ggagtacgca cgcaccatcc ttggcctcga gggagcgtcc agctcggagt tcgatcccga
     1441 gaccccgcac cccgtcatcg cgacgatgga ggagcagctt gagttcgttg agggcgctgg
     1501 cgacctgggc ggcacgatgc gtctgggcga gtaccgcgcg gtgctgaccc ccggttcggt
     1561 cgtcgagcag acctatggtg cggcgacggt gggcgagcgt catcgccacc gctacgaggt
     1621 caacaacttc taccgcccgc agctcgaaga ggccggcctg gtggtgtcag gggagtcgcc
     1681 cgaccgtcag ctggtggagt tcatcgagct gccgcgcacg gcacacccgt actacgtggc
     1741 tactcaggcg caccccgagt tcctctcgcg ccccaccaag gctcacccac tgtttgccgg
     1801 gctcattggc gccgcgctcg agcgtcagaa ggacgcatcg gccaccgagc aagctcctgc
     1861 cgctcaggac gtcgcgcccg cagcctcatc ggcatcgtga ccgacgagcc gcgcggcgaa
     1921 ctcgccgacg tggccgcccc gcggcccacg ctggcgcgcc acgagcgctt ccagggccgc
     1981 gtgtggaacc ttgtgagcga cgatgtggag cttggccatt ctcaggtgac gagcgacttt
     2041 gtgcagcaca cgggagccgt ggccatagtg gcacttaacg actccgatga aatgtacctg
     2101 gtgcggcagt accgccaccc cgtcggcgtg gagtgctggg agccgccggc cgggctactc
     2161 gacgtcggtg acgagttgcc cgtcgaggcg gccaagcgcg aactccatga ggaggccgac
     2221 ctcaccgcgg ccacctggca tgtgctcgcc gactacttcc cgtccggtgg cggcagctcg
     2281 gaggccgtga gggtcttcct tgcgagggac ctggcagctg ttcccgaggc cgaaaggcac
     2341 gtgcgcaccg acgaggagca cgacatggtg ggcgcgtggg tgccgcttga tgagattctt
     2401 gccgcgatcg ccgcgggtcg cgtgcacgca tcgtccctcg tggtgggggc catgg
//
LOCUS       DASRKH010000467         4583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_12323, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000467 DASRKH010000000
VERSION     DASRKH010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12323"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1286
                     /gene="thrS"
                     /locus_tag="VFC06_07510"
     CDS             <1..1286
                     /gene="thrS"
                     /locus_tag="VFC06_07510"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017823709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HZL81762.1"
                     /translation="DELLAYKDRLAEAERRDHRRLGADMDLFSFPDEIGSGLAVFHPK
                     GGIIRMEMEEYSRKRHIEEGYEFVYSPHITKAQLFQTSGHLDWYADGMYPPMHMDEER
                     DAAGNITKQGQDYYLKPMNCPMHNLIFRSRGRSYRELPLRMFEFGTVYRNEKSGVVHG
                     LTRARGFTQDDAHIYCTREQMKSELTTTLNFVLGLLKDYGLTDFYLELSTKNPEKFVG
                     DDDAWEEATETLRQVGLESGLEIRDDPGGAAFYGPKISVQTRDAIGRSWQMSTIQLDF
                     NLPERFELEYTAADNSRQRPVMIHRALFGSIERFFAILTEHYAGQFPVWLAPVQVKAI
                     PVAEAFDDYLADIAAQLRAKGVRVEVDTSDERFPKKIRTASKEKVPFVLIAGGEDVEA
                     GAVSFRLRDGSQDNQVPVDVAVARIVESIETRAQV"
     gene            1313..1837
                     /locus_tag="VFC06_07515"
     CDS             1313..1837
                     /locus_tag="VFC06_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018135717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HZL81763.1"
                     /translation="MGGQPDGFERLWTPHRMAYVKSASERPDMEGTHQCPLCKTPDDN
                     DRDHLIVHRGTDCYVVLNLYPYNPGHLMICPYRHVGWYTEITDAERDEMSALTQTGMR
                     VLRHLTGTEGFNVGINQGKTGGAGIHEHLHQHVVPRWVGDSNFLPIIARTKPLPELLD
                     DTWQRVSEAWAKTG"
     gene            1858..2520
                     /locus_tag="VFC06_07520"
     CDS             1858..2520
                     /locus_tag="VFC06_07520"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HZL81764.1"
                     /translation="MFGKLRPLMAAIWRAPASALVKAGVHPNAVTIVGTVGVVVGAIV
                     FLPRGGTALFWGVLIIAFFVVTDMLDGTMARLSGKSSRLGAYLDSTLDRMADAAIFGA
                     LVWGFRSDEPATALAALMCLTLGSFVPYAKARAASLGIVTMNGIAERSDRLAIGLTAT
                     GLVGLGLPLVVLTVTLWLLAAAAAFTVGQRTWSVIKVQMEENRAGGETPSPSAPADRA
                     SS"
     gene            2520..3410
                     /locus_tag="VFC06_07525"
     CDS             2520..3410
                     /locus_tag="VFC06_07525"
                     /inference="COORDINATES: protein motif:HMM:NF005919.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol mannoside acyltransferase"
                     /protein_id="HZL81765.1"
                     /translation="MNSFVVAWRVSRRVPVSVIRGAAWLIAMYGWVRNAKPARRLEQN
                     LHRVTGLEGRELRRLSRKAMASAARYYSEVLELPRMKGEVIDARVRVEGLEDVEWVLE
                     REHGTVAVLGHSGNWDLIGAYACRNIIKITSVAEVLEPREVYDEFVAFRENLGMQILG
                     HEGGATFRQLLRIAKSEHTLVCLLSDRDLSGHGVTVSMWGQQARVAPGPAALALAART
                     ALIPVSVHYERLSGARRRAAKSRWGIVMTFGDPLLPEDFTGSDKVGTMTQAWAEPFSR
                     GIAAHPEDWHMLQRFGWAEV"
     gene            3411..4562
                     /locus_tag="VFC06_07530"
     CDS             3411..4562
                     /locus_tag="VFC06_07530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZL81766.1"
                     /translation="MRIGIVCPYSLDRPGGVQLHVIDLAEALIKRGHQVSVLAPAAKG
                     TSVPDYVKTAPGSIALRYNGSTARVTFGLITAARVRRWMADGEFDVVHLHEPGAPSLS
                     VIAMWARLGPTVATFHTANDDSRLMRIGKPWIKPGFEELDARIAVSPSAAQTVKAHLR
                     VEATHIIPNGVDTSAYVNAKPREEWTGTAVAPTVGILGRLDEPRKGLDDFLASIPLVR
                     EKHPRARFLVAGRFSDRAAARIARGGGEVVGELDEAQKAEFMSSVDVYCAPNTGGESF
                     GIVLVEAMAAGAAVVASDLVAFRDVATSDDGEGCAAFFPVGDAQGLADQVCALLDDPV
                     ERAALAKRGCARAGTFDWHQVAPRIEETYRDAIRLYASFFPQRVKETPA"
BASE COUNT          798 a         1446 c         1527 g          812 t
ORIGIN      
        1 aggacgaact gctcgcgtac aaggatcggc tcgccgaggc cgaacgccgc gatcaccgca
       61 ggcttggcgc ggacatggat ttgttctcct tccccgacga gatcggctcg gggctcgcgg
      121 tgttccatcc caagggcgga attatccgta tggagatgga ggagtactcg cgcaagcgcc
      181 acatcgagga gggctacgag ttcgtctact cgccccacat caccaaggct cagctcttcc
      241 agacctcggg gcacttggac tggtatgccg atggcatgta ccccccgatg cacatggacg
      301 aggaacgcga cgcggcggga aacattacga agcagggcca ggactactac ctgaagccca
      361 tgaactgccc gatgcacaat ctgatcttcc ggtcgcgcgg acgttcgtac cgcgaactgc
      421 cgctgaggat gtttgagttt ggcaccgtgt accgcaacga gaagtcgggc gtggttcacg
      481 gcctgactcg tgcgcgcggc ttcacccagg acgacgccca catctactgc acgcgcgagc
      541 agatgaagtc ggaactgacc accacgctga actttgtctt gggcctgctc aaggactacg
      601 gcctgaccga cttctacttg gaactgtcga ccaagaaccc ggagaagttc gtgggtgatg
      661 acgatgcctg ggaagaggcc accgagacgc ttcgccaagt gggtctggaa tcggggcttg
      721 agatcaggga cgacccgggc ggcgccgcct tctacggccc caagatttcg gtgcagacgc
      781 gagacgcgat cggccgctcg tggcagatgt ccaccattca gttggacttc aatctgccgg
      841 agcgattcga gctcgagtac acggctgcag acaactctcg ccagcgtccg gtgatgatcc
      901 accgcgcgct gttcggctcc atcgagcgct tcttcgcgat cctgaccgag cactatgcgg
      961 gccaattccc tgtgtggctc gcgcccgttc aggtgaaggc gatccctgtg gccgaggcgt
     1021 tcgacgacta cctcgccgac atcgccgccc agttgcgcgc gaagggcgtg cgggtcgagg
     1081 tcgacacctc cgacgagcgt ttccccaaga agattcgcac cgcgtccaag gagaaggtcc
     1141 ccttcgtgtt gatcgctggc ggcgaagacg tcgaggccgg cgccgtgtcg ttccggctac
     1201 gggacggcag ccaggataat caggtgcccg tggatgtcgc agtggcgcgc atcgtcgaat
     1261 cgatcgagac aagggcgcaa gtctgatggt ggagtgggcc gacgcagaga cgatgggcgg
     1321 gcagcccgac gggttcgagc gcctgtggac gccgcatcgc atggcgtatg tcaagagcgc
     1381 gtcggagcgt ccggacatgg aaggcaccca ccagtgcccc ctgtgcaaga cgcctgacga
     1441 caatgaccgc gaccacctga tcgtgcatcg gggcaccgac tgctacgtgg tgctcaatct
     1501 gtacccgtac aaccccggcc atctgatgat ctgcccgtat cgccacgtag gctggtacac
     1561 ggagatcacc gatgccgagc gcgacgagat gtctgcgctg acccagactg gaatgcgcgt
     1621 gcttcggcac ctgacgggaa ccgaaggttt caacgtcggc atcaatcagg gcaagaccgg
     1681 cggcgccgga atccacgagc acctgcacca gcacgtggtg ccgcggtggg tcggggattc
     1741 gaattttcta ccgatcatcg cgcgcaccaa gccgttaccc gaactattgg atgacacgtg
     1801 gcagcgcgtc agtgaggcgt gggcaaagac cggctagcgg tccaacaagg agaagacatg
     1861 tttggcaagc tgcggcccct gatggccgca atctggcggg ctcctgcgag cgcgctcgtc
     1921 aaggcgggag tgcaccccaa cgcggtcacg atcgtgggaa ctgtcggcgt tgtcgtgggt
     1981 gccatcgtgt tcttgcctcg cggcgggacc gcgctgtttt ggggcgtcct catcatcgcc
     2041 ttctttgtgg tcacggacat gcttgacggc acgatggcga ggctgtccgg taagagcagc
     2101 cgcctcggtg cgtacctcga ctcgactctc gaccgcatgg cagatgccgc catcttcggc
     2161 gcgctcgtgt ggggctttcg cagtgacgag ccggcgacgg cgctcgccgc tctcatgtgc
     2221 ctgacacttg gttcctttgt gccgtacgcg aaggcgcgcg ccgcgagcct gggcatcgtg
     2281 accatgaacg ggatcgcgga gcgttccgat cgcctcgcga ttgggctgac cgcaactggc
     2341 cttgtcggtc tgggtctgcc gctcgtggtg ctcaccgtca ccttgtggct gctcgccgcg
     2401 gcagccgcct tcacggtcgg tcaacgcacg tggtcggtca tcaaggtcca gatggaggag
     2461 aaccgtgcgg ggggcgagac tccctcccca tcggcgcccg cggatcgcgc ttccagctga
     2521 tgaattcctt cgtcgtggcg tggcgggtgt cgcgccgggt gcccgtcagc gtgatccgcg
     2581 gtgccgcatg gctcatcgcg atgtatggct gggtcaggaa cgcgaagccg gcgcggcgtc
     2641 tcgaacagaa cctgcatcgc gtgaccggct tggagggtcg tgagctccgc cgcctcagcc
     2701 gcaaggcgat ggcctcggct gccaggtact actccgaggt gctcgaattg ccccgcatga
     2761 agggcgaggt cattgacgcc cgcgtgcggg tggaaggcct tgaggacgtc gaatgggtct
     2821 tggagcgaga gcacggcacg gtcgcggtgc tgggacactc gggcaattgg gacctgatcg
     2881 gggcgtatgc atgccggaat atcatcaaga tcacctcggt ggctgaggtg cttgagccgc
     2941 gcgaagttta cgacgagttt gtggcattcc gtgagaacct cggcatgcag attcttggcc
     3001 acgaaggcgg cgcgacgttc cgtcaactgc tccgcatcgc caagtcggaa cacactctcg
     3061 tctgtttgct gagcgaccgc gacctttccg ggcacggcgt cacggtgtcc atgtggggcc
     3121 agcaggcgcg agtggctccg ggccctgccg cgctcgccct ggcggcccgc accgcgctca
     3181 tacccgtctc ggttcactac gaacgcttga gcggggcgcg ccgccgcgcg gccaagtcgc
     3241 gatggggcat cgtgatgacc tttggggacc cgctcttgcc agaagacttc accggaagtg
     3301 acaaggtggg gaccatgact caggcatggg ccgagccctt cagtcgcggg atcgcggcgc
     3361 acccggagga ttggcacatg ctccaacgat tcggctgggc ggaggtctag gtgcgcatcg
     3421 gcatcgtgtg tccctactcg ttggaccggc cagggggagt gcaactccac gtcattgacc
     3481 tcgccgaggc actcatcaaa cgcggccacc aggtatccgt gctcgctccc gccgcgaagg
     3541 gcacgtcggt tccggactac gtcaagacgg cacccggatc tattgccttg cggtacaacg
     3601 ggtcgacggc gcgcgtcacc ttcgggctca tcaccgcggc gcgggtgcga cgttggatgg
     3661 cggacggaga gttcgacgtc gttcacctgc acgagccggg ggcgccctcc ctcagtgtga
     3721 ttgccatgtg ggctcggttg ggccccacgg tggcgacgtt ccacaccgcc aacgacgact
     3781 cgcgcctgat gcgaatcggc aagccatgga tcaagccggg cttcgaggag ctcgacgccc
     3841 gcatcgcggt gtcgccctcg gccgcccaga ccgtgaaggc tcacttgcgg gtcgaggcca
     3901 cgcacatcat ccccaatggc gtggacacgt ccgcctacgt gaacgcaaag ccccgcgaag
     3961 aatggacggg aacggcagtg gctcccaccg tcggcattct cggcaggttg gacgagcctc
     4021 gcaagggtct cgacgacttc ctcgcatcca ttccgctggt acgggagaaa cacccacgtg
     4081 cccggttcct cgtggcaggg cgtttctccg accgcgccgc ggcacgcatc gcgcgtggcg
     4141 ggggtgaggt cgtgggcgag ctcgacgaag cccagaaggc cgagttcatg tcctccgtgg
     4201 acgtgtactg cgcgcccaac acaggtggag agtcgttcgg catcgtcctc gtcgaggcca
     4261 tggcggccgg cgcggccgtg gtcgcgagcg atttggtggc cttccgcgac gtcgcgacct
     4321 ccgacgacgg cgaaggatgc gcggcgttct tccccgtggg cgacgcccaa ggtctcgcgg
     4381 accaagtgtg tgccttgctg gacgaccccg tggagcgcgc ggcgctcgcg aagaggggtt
     4441 gtgcacgcgc aggcaccttc gactggcacc aggtggctcc ccgcattgag gagacatacc
     4501 gtgacgcgat tagactgtac gcgagcttct tcccccaacg agtgaaggaa accccagcat
     4561 gagcgacacc gcagcatccg cta
//
LOCUS       DASRKH010000468         3372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_1456, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000468 DASRKH010000000
VERSION     DASRKH010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1456"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            459..1526
                     /locus_tag="VFC06_07535"
     CDS             459..1526
                     /locus_tag="VFC06_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009884440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HZL81767.1"
                     /translation="MVNDNQIKVYGGIDTHADTHHVAVIDAAGRRLADVQVPTTAPGY
                     QAALRFLGSWPTLVSVGIECTGSYGAAVTRAVREAGIGVFEVNRPNRFDRHLRGKTDV
                     FDAYSAAEAVLSGRATAAPKGGDGLVEALRVLRTSRSSALSARTATINQIKGMLVTAP
                     EQLRARYRGLSNTKLIAALAASRPTATPTTAAEAAAYSLRLLARRWLALTEQIEDLAG
                     HMQRLLDDHAPALMSVFGCGQDTIAQLLITAGDNPDRLRSEAAFASLAGACPIPASSG
                     KTNRHRLNRAGDRQANSALYHVIIVRLRYDKETQDYAARRTAEGKTKMEIIRCLKRYL
                     VRQLYPLIVQTLQPRPEALTT"
     gene            1661..2995
                     /locus_tag="VFC06_07540"
     CDS             1661..2995
                     /locus_tag="VFC06_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HZL81768.1"
                     /translation="MPEPTLCCRAGDDYCGRCDVLVGLDGLRVVAVECDVGTGALTVM
                     VESGPEVMGCHSCGVVAHGHGRVKVRLVDAPAMGRPVTIIWRKRRWVCPEPSCPVGSF
                     VEQDESIALPRTKLTTRACWWAIGQLRREHASVNGLRRQLGTGWRTVWESIRPLLEVA
                     DADQSRFAGVTILGVDEHVWHHVSTKPIGDGGRGPKELTGMVDLSRDQDGRTHARLLD
                     LVPGRSGQAYRDWLASRSDDFREGVQIATLDPFHGYKNAIDDQLEDAIAVLDAFHVVK
                     LATQAVDEVRRRTQHEIHGHRGRKDDPLYRIRNILHAGEENLTDRQRDRLERAWAVDE
                     RHIAVEVAWKCAQLVRSVYHQDTHAAGRAIAEHLLETLPSCPIPEIARLGRTLRQWRE
                     AFLGYFDTSGASNGGTEAINGLIELHRRVARGFRNYDNYRLRMLLIGGGLQL"
     gene            complement(3004..>3372)
                     /locus_tag="VFC06_07545"
     CDS             complement(3004..>3372)
                     /locus_tag="VFC06_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HZL81769.1"
                     /translation="VNGLRRQLGTGWRTVWESIRPLLEVADADQSRFDGVTILGVDEH
                     VWHHVSTKPIGAGGRGPKELTGMVCLTRDRTGRTRARLLDLVPGRSGQAYRDWLASRS
                     DDFREGVQIATLDPFQALRR"
BASE COUNT          615 a         1091 c         1080 g          586 t
ORIGIN      
        1 cacgaccttg ctgtgccctt cacggtgacg ttgtctagcg agtcacgggc atcgtcattc
       61 accttcgagc gccggtacag aaacaccgcg gcaatcatcg cggcgatcgg cgacatcacg
      121 gcgatggtgg cctggatgac gtcagtaacc gtagtcgcct gctttaccgg ctcttcacta
      181 tggatctcgt ggaccggttg gtctggctct ttgatggacg tgacggcttc ggcccgttgg
      241 acgcaggatg gtctggtggg cgggtctgac tcacggctct tctgacgtca ccggttggtg
      301 actgtcctgc atgcgacgtg tcgaccggct cggctcactt tcgaagtgca gcccggcggg
      361 ccgtcatgtc ctgataggag tctggcaagt ccccgatagt gaactgactg acgcccggcc
      421 gggctgcgca cccgacaata cggaagggcg cagttcctat ggtgaacgac aatcagatca
      481 aggtctacgg cgggatcgac acgcacgcag atacacatca tgtcgcggtg atcgatgccg
      541 ctgggcggag gctggccgat gtccaggtgc caaccaccgc tcctggctat caggcggcgt
      601 tacggttcct gggttcgtgg cccacgctgg taagcgtggg tatcgagtgc accggctcct
      661 acggggccgc agtgacccgc gccgtgcgtg aggcgggcat cggagtgttc gaggtgaacc
      721 ggcccaaccg atttgatcgc cacctccggg gcaagacaga cgtcttcgac gcctactcgg
      781 ctgccgaggc ggtgctgtct gggcgtgcaa cggctgcacc gaaggggggc gatggtctgg
      841 tcgaagcact gcgggtgctg cgcacgtcga ggtcgtcggc actgtcagcc aggaccgcga
      901 cgatcaacca gatcaagggc atgctggtca ccgcgcctga gcaacttcgt gcacgctacc
      961 gcggcctgtc gaacaccaag ctgatagccg cgctggcggc gtcacggccg accgcgacac
     1021 caaccaccgc ggccgaggcg gcggcataca gcctgcggct gctggcgcgg cgctggttgg
     1081 ctctgactga gcagatcgaa gacctcgccg ggcacatgca acgactgctc gacgaccacg
     1141 cccctgccct gatgagcgtg ttcggctgcg gccaggacac catcgcccag ttgctgatca
     1201 ccgcaggcga caaccccgac cggttgcggt ctgaagcggc cttcgcctca ctggccggcg
     1261 cttgcccgat ccctgcctca tcgggcaaga cgaaccgtca ccgactgaac cgggcaggtg
     1321 accgtcaagc gaactcagcg ctctatcacg tcatcatcgt gcgcctgcgc tacgacaaag
     1381 agacccagga ttatgccgcg cgacgcaccg cggagggcaa gacgaagatg gagatcatcc
     1441 gctgcctcaa acgctacctc gtccgccagc tctacccact catcgtgcaa accctgcaac
     1501 caagacccga agcactcacc acttgacagc gacctatagg agtgtcgccg ttgagtgtgg
     1561 ggtgagggcg gcgcgccggg tggcgggttg agggttggag gggtcgaggt ccccgatgat
     1621 cagaggactt ctaactcttc cgatcgtgag gacctcgaca atgcccgagc ctacgttgtg
     1681 ttgccgtgct ggtgacgact attgcgggcg ttgcgacgtg ctcgtcggac tcgacgggct
     1741 gcgtgtggtc gcggtcgagt gtgacgtggg caccggggcg ttgaccgtga tggtcgagtc
     1801 cggcccagag gtgatgggct gccactcgtg tggggtggtt gctcacggac acggccgggt
     1861 caaggttcgc ctcgttgacg cgccggcgat gggacgcccg gtcacgatca tctggcgtaa
     1921 acgccggtgg gtatgtcctg aaccgtcgtg tccggtgggt tcgttcgtgg aacaggacga
     1981 gtcgatcgcg ttgccacgca ccaaactgac tacccgtgca tgctggtggg cgatcgggca
     2041 gcttcgtcgc gagcatgcct cggtcaacgg gcttcgccga caacttggca cgggctggag
     2101 gacggtgtgg gagtcgatcc gtcccctcct tgaggtggcg gacgcggacc agtcccgctt
     2161 cgccggtgtc acgatcctcg gtgtcgatga acacgtgtgg catcacgtgt ccaccaagcc
     2221 catcggggac ggtggtcggg gtccgaagga actgacgggg atggttgatc tcagtcgtga
     2281 ccaggacgga cgcacccatg cacgcttgct cgacctggtc ccaggccgtt ccggacaggc
     2341 gtaccgggac tggctagcca gtcgcagtga cgacttccgt gagggtgttc agatcgccac
     2401 cttggaccca ttccatgggt acaagaacgc gatcgacgac caactcgagg atgccatcgc
     2461 cgtgctcgac gcgttccacg tcgtcaaact cgccacccaa gccgtcgacg aggtacgccg
     2521 acgcacgcaa cacgagatcc atggtcaccg cggacgcaaa gacgacccgc tctaccggat
     2581 ccgcaacatc ctgcacgccg gtgaagagaa cctcaccgac cgccaacgtg accgacttga
     2641 gcgggcgtgg gcggtcgacg aacgccacat agccgtcgaa gtcgcatgga agtgcgctca
     2701 actggtccgc tccgtctacc accaagacac tcacgctgcc ggacgtgcca tcgccgaaca
     2761 cctcctcgag acgctcccat cgtgtccgat tcccgagatc gcccgcctgg gccggaccct
     2821 gcgccagtgg cgtgaagcgt tcctgggcta cttcgacacg tccggagcca gcaacggcgg
     2881 caccgaagcc atcaacggcc tcatcgaact ccaccgccgc gtcgcccgcg gcttccgcaa
     2941 ctacgacaac taccgactac gaatgctcct catcggcgga ggcctccaac tatgacccca
     3001 cactcaacgg cgaagagcct ggaatgggtc caaggtggcg atctgaacac cctcacggaa
     3061 gtcgtcactg cgactggcta gccagtcccg gtacgcctgc ccggaacggc ctgggaccag
     3121 gtcgagcaag cgtgcacggg tgcgaccagt gcggtctcgg gtcaaacaga ccatgcccgt
     3181 gagctctttt ggaccccgcc cgccagcacc gatgggcttg gtggacacgt gatgccacac
     3241 gtgttcatcg acgccgagaa tcgtgacgcc atcgaagcgg gactggtccg cgtccgccac
     3301 ctcaaggagg ggacggatcg actcccacac cgtcctccag cccgtgccga gttgtcggcg
     3361 aagcccgttg ac
//
LOCUS       DASRKH010000469         2124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_57124, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000469 DASRKH010000000
VERSION     DASRKH010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57124"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="VFC06_07550"
     CDS             <1..1002
                     /locus_tag="VFC06_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HZL81770.1"
                     /translation="ERNNYLPEIEDLADEAVAAVGHTGGALTHRSVTEMADRLGLSIV
                     HVDDLPHSARAVLDLEQGRIYIPPASIPGGHGLRSLALQALAHIALGHGDPVDYADFL
                     RQRLEASYYAAACLMPRKPSIAFLQRAKDERRIAIEDFRDAFGVTHEAAALRFTNLAT
                     EHLGITLHFLRATGGGAIERAYENDSLPLPVDVTGSMEGEIVCREWSARRAFARATRT
                     TEYHQYTDTPVGTFFESTQTGAGPAGEYSITLGVPYAESKWFRGRETVNRSTSRCPDV
                     SCCRAPDDGLVARWHGQAWASARVHAHTFSPLPHGTYPGVDDRELYDFLEAHGREET"
     gene            complement(1027..1374)
                     /locus_tag="VFC06_07555"
     CDS             complement(1027..1374)
                     /locus_tag="VFC06_07555"
                     /inference="COORDINATES: protein motif:HMM:NF017880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HZL81771.1"
                     /translation="MEIWHLALAEDWDEAVATGSYRVSTLGRTLAQEGFIHCSRPEQA
                     PGVAARFYADVTAPLRILVMDDDRVRASGVEVKYEDAGNGELFPHIYGAIDPAWVVEA
                     RSARMKDGTLHVA"
     gene            1565..1729
                     /locus_tag="VFC06_07560"
     CDS             1565..1729
                     /locus_tag="VFC06_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81772.1"
                     /translation="MTRDGTRFGEAADADGRAAIDGRVEDVLAGVPALENTGTVDLPY
                     ETHVYRAVAP"
     gene            1850..1978
                     /locus_tag="VFC06_07565"
                     /pseudo
     CDS             1850..1978
                     /locus_tag="VFC06_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867582.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            complement(1970..2124)
                     /locus_tag="VFC06_07570"
                     /pseudo
     CDS             complement(1970..2124)
                     /locus_tag="VFC06_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018768610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
BASE COUNT          368 a          710 c          682 g          364 t
ORIGIN      
        1 gagcggaaca actacctgcc cgagatagag gacctcgcgg acgaagccgt cgctgcggtc
       61 gggcacacag gcggcgcgct cacccaccgc tcggtcactg agatggccga ccgcttgggg
      121 ttgtcgatag tccacgtcga cgaccttcct cactccgcac gcgccgtgct ggaccttgag
      181 cagggacgga tctacatccc gcctgcctcg attccgggcg gtcacgggct ccgctcgctc
      241 gcactccagg ccctcgcgca tatcgcgctg ggacatgggg atccagtgga ctatgcggac
      301 ttcttgaggc agcgactcga ggccagctac tacgcggcag cgtgcctcat gccgagaaag
      361 ccctccatcg cgtttcttca gcgtgccaag gacgagcgac gcatcgcgat tgaggacttc
      421 cgcgacgcat tcggcgtcac gcatgaggcg gcagcgctgc gcttcaccaa cctcgccacc
      481 gagcatctcg gaatcacgct gcactttctt cgcgccaccg gaggcggagc gatcgagcgc
      541 gcctatgaga acgactcgct gccactcccg gtggacgtga cggggtccat ggagggggaa
      601 atcgtgtgcc gggagtggag cgccaggcga gcgttcgcgc gggcaacgcg caccaccgag
      661 taccaccagt acacagacac gcccgtcggg acgttctttg agtcgactca gacgggagcg
      721 ggaccggccg gcgagtactc gatcacactc ggcgtgccat acgcggaatc caagtggttc
      781 cggggccggg agaccgtcaa ccgctccacg tcgcgctgcc ccgatgtcag ctgctgccga
      841 gcaccggacg acggtctcgt cgctcggtgg cacggccaag cctgggcgag cgctcgtgtc
      901 cacgcccaca ctttctcacc gttgccacat ggcacctacc ctggcgtcga tgaccgcgaa
      961 ctctacgact ttctcgaggc gcatgggcgc gaggagacct gatgggcgat ggcccggcac
     1021 gcgcggtcag gccacgtgga gcgtcccatc tttcatgcgc gctgagcgcg cttcgaccac
     1081 ccaggcggga tcgatcgcgc cgtagatgtg ggggaagagt tcgccgttgc cggcgtcctc
     1141 gtacttgacc tcgacgccag aggcccgtac tcgatcgtca tccatgacca gtatccgcag
     1201 cggcgcagtg acgtccgcgt agaaccttgc cgcgacgcct ggcgcctgct cggggcgcga
     1261 gcagtggatg aagccctcct gcgctaacgt gcggcccagg gtggacacgc gatacgagcc
     1321 ggtcgccact gcctcgtccc agtcctctgc gagggcaagg tgccagattt ccatgcctcc
     1381 atcgtggcat cgatggccgg ctcatgagtc gaccgacagc ctgcgccgtt caggattcac
     1441 gtgcggcgtc cttagactgg tggccatgaa gcaagtcgtc gccttgcccc agattcagta
     1501 ctccgaggtg gtgcagaacg accctgtgaa ggccctgccc gccgccgtga agtcggggac
     1561 gtagatgacg cgcgacggca cgaggtttgg cgaggcggcc gatgccgatg gccgggccgc
     1621 gattgatggt cgcgtggaag acgttctcgc tggggttccc gccctcgaga acaccggcac
     1681 cgttgacctg ccctacgaga cgcatgtgta tcgcgcagtc gcgccctagg cccgcgcact
     1741 ggcccccaac cggcgtcgga cgggccttgt cgcggccgga ctgcgaatag acgggtagaa
     1801 taaggaggct ccgttctgag cccacccctc atcgatgaaa agagtcccta tgtccgtgcg
     1861 ctcagacctg cgcaacgtag ccatcgtcgc ccacgtcgac catggcaaga ccactcttgt
     1921 cgatgccatg ctccgtcagg gtggtgccta tggtgaacac gcccacgtcg aggatcgtga
     1981 tgcccttttc acgttccagg tcgccagagt ccatcgcgcg atcctcgacg tgggcgtgtt
     2041 caccgtaggc accaccctga cggagcatgg catcgacaag agtggtcttg ccatggtcga
     2101 cgtgggcgac gatggctacg ttgc
//
LOCUS       DASRKH010000470         3499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_32471, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000470 DASRKH010000000
VERSION     DASRKH010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32471"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            287..430
                     /locus_tag="VFC06_07575"
     CDS             287..430
                     /locus_tag="VFC06_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPGG-motif small membrane protein"
                     /protein_id="HZL81773.1"
                     /translation="MGTILWILAVILVVSGIVALVRRQFLWGIILIIVGLLVGPGGVS
                     LFT"
     gene            523..714
                     /locus_tag="VFC06_07580"
     CDS             523..714
                     /locus_tag="VFC06_07580"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HZL81774.1"
                     /translation="MGHNVDETKGKIKQAAGDLTGDKDLVREGKVDEAAGKAKGFLED
                     VGDKVGHVGDEIKDRLTKD"
     gene            1205..>3499
                     /locus_tag="VFC06_07585"
     CDS             1205..>3499
                     /locus_tag="VFC06_07585"
                     /inference="COORDINATES: protein motif:HMM:NF015588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 65 glycosyl hydrolase"
                     /protein_id="HZL81775.1"
                     /translation="MNRHENQPMAWDDAGPGGYPSDGQPGGSPWVLRFDGFEPAAEGR
                     REALCTLGNGYWGTRGAAPEADADSVHYPGSYLAGVYNRVITTIGSRSVEDEHMVNAP
                     NWLVMRFRTADGEWYRPRGPELLHHVQELDLRRATLTRTMRWRDDSGRTTRVTSCRFL
                     SQAEPHVGVLTTTVEAEDWSGPMTFQSALEGRVANRNAVGDRMLTGVHLTPRGTAELN
                     GETLLLEVETNQSGIHIAMAARTRVLRDDVVVDPVRRYLFDDAGWVAHEFVVDLQPGR
                     PVTVEKCVTVATSRDRAVASPARAVATWMTRLADRPDLVAAHERQWQILWDEFAVDIP
                     DGSHEALALNLNTFHVLQTLAEANLDLDAGIPARGLHGEGYRGHVFWDEMFVYPILTL
                     RRPDLSRALLGYRYRRLNEARARARAAGHAGAMFPWQSGIDGSEVTPTELYNPHSLRW
                     QDDFSHRQQHVGLAIAYSVWHYYQSTAATDFMIHQGAEMLIEVARYFADLTTHDEATD
                     RYDIDGAMGPDEFHDGYPGKPGQGMRNNAYTNVMTAWVLRRAIETVSLLEGRNCRPLW
                     NRLALHPDEIDRWDRISRRLRVPFHADGVISQFEGYEDLPEFDWQGYRARHDSFAALG
                     MVLAAEGDSTNNYRIAKQADVLMLLYLFSAEELRAQLQEMGYSLPPEAVVRTVDFYAS
                     RSTHGSTLSNVAHSWVEARRDREQSWQFLQRALQSDLADIQGSTTQEGIHMGAMAGSV
                     DMVVRCYTGLEIRNDMLWLHPVLPA"
BASE COUNT          617 a         1138 c         1161 g          583 t
ORIGIN      
        1 gacgggactg ggcggtctgg gggtcaaggg gtcgtaggtt cacatcccgc cggcccgacg
       61 tatcgtcgca gttcatgggc ctgatcgcat tgcgggcggg ccgtttcgcc acccttgaca
      121 tcagagttga aatcgagtga cgcgcctgac gtcaacatcg caggctcgcc tgcgggtttc
      181 gcaacaagac ccactaccgc ctcctgtgct ccgcgtacat cttccgcaag gctgacgaac
      241 ggggtacttt gaacctactg gcaatggtgt cggaaggagg aacatcatgg gtaccatcct
      301 gtggatcctg gcagtgatcc tggttgtctc agggatcgtc gcattggttc ggcgtcagtt
      361 cctctggggc atcatcctta tcatcgtggg actgctagta ggcccgggcg gggtcagtct
      421 cttcacatga cgaaatggcc gcatgcgccc tcgtggcatg tgcggccgtt cggatttcaa
      481 tccatcgtga gtacagattc cgacaactgg aggagacgca tcatgggtca caacgtcgat
      541 gagacgaagg gcaagatcaa gcaggcggcc ggcgacctca ctggcgacaa ggacctcgtg
      601 agggagggga aggttgacga ggctgccggc aaggccaagg ggttcctgga ggatgttggt
      661 gacaaggtcg ggcacgtcgg tgacgagatc aaggacaggc tcaccaaaga ctgaccggcg
      721 cagttggcag gggctcgcag gtccgtatgg gatgcctgac ggcatctctc cccttgcttg
      781 accccatccc cgaaagcgcc cgctggcccg agctccatcg caggctcgcc cgcgggtttc
      841 gcaaaaggac ccactaccgc ctccgcatga tcctcgccgg aggaggactc cccccaccaa
      901 ttctgtgttg agccggcagg agcgtcggac catcgctctc aggggcgcgg gggacgtcgc
      961 ggcgaacaac agatccctga caagggacat cacgcagcga ctcgtctcgg ggttgtgact
     1021 ccctcacaag gcccgtagcc gatccgatca gtgcctattc gctgtttggt gacggctctt
     1081 ggattcccgg catggtctgg gcgatcatct tgcggattgt ggtgaggtcg cgtttgacgg
     1141 tcgtgggtga gtcgcactga cggagattgt cataccatgg ggagacgatg agatcgggca
     1201 atccatgaac cgccatgaga accagcccat ggcgtgggac gacgcaggcc cgggcggata
     1261 cccgtcggac ggacaacccg gcgggagccc gtgggtgctc cgcttcgacg gtttcgaacc
     1321 cgccgcagaa ggccggaggg aagccctgtg caccctcggc aacggctatt gggggacacg
     1381 cggcgcggcc cccgaggcgg acgccgactc agtgcactac cccggctcct atctcgcggg
     1441 cgtctacaac cgggtgatca cgaccatcgg cagtcgatcg gtcgaggacg agcacatggt
     1501 caacgcgccg aactggttgg tcatgcggtt caggacggct gatggcgagt ggtaccggcc
     1561 gagggggccg gagctcctgc accacgtcca ggagctcgac ctgcgccgcg cgacgctgac
     1621 caggacgatg cgctggcgtg acgactcggg gcggaccacc cgtgtcacct cgtgtcgttt
     1681 cctctcgcag gccgagccgc acgtcggggt gctgaccacg actgtcgagg ccgaggactg
     1741 gtccggtccg atgacgttcc aatcggccct cgagggacgc gtcgccaacc ggaatgcggt
     1801 gggcgaccgg atgctcaccg gtgtccacct caccccgcgg ggcacggctg aactcaacgg
     1861 cgaaaccctg ctgctcgaag tggagaccaa ccagtccggg atccacatcg cgatggccgc
     1921 gcggacgcgg gtcctccgcg acgacgtcgt ggtggacccg gtgaggcgct acctgttcga
     1981 cgacgccggg tgggtcgcgc atgagttcgt ggtggatctc cagccggggc gccccgtcac
     2041 cgtggagaag tgcgtcaccg tcgccacatc gcgcgaccgt gccgtcgcgt cacctgctcg
     2101 cgcggtggcg acgtggatga cgcggctcgc tgatcgcccc gacctcgtcg cggctcacga
     2161 acgccaatgg cagatcctct gggacgaatt cgcggttgac atccccgacg gttcgcacga
     2221 ggccctcgcc ctgaacctca acacgttcca cgtcctccag acgctggccg aggcgaacct
     2281 ggatctcgac gccggcatac cggcgcgcgg cctgcacggc gagggatacc ggggacacgt
     2341 gttctgggac gagatgttcg tgtacccgat actcaccctc cgccgcccgg acctgagccg
     2401 ggcgctgctg gggtaccgct atcgccgcct gaatgaagcg cgtgcacgcg cccgtgcggc
     2461 cggccacgcg ggcgcgatgt tcccgtggca gagcggcatc gacggcagcg aggtgacgcc
     2521 gaccgagctc tacaatccac acagcctccg gtggcaggac gacttctccc accgccaaca
     2581 gcatgtcggg ctcgcgatcg cgtacagcgt gtggcactac taccagtcca ccgcggccac
     2641 ggacttcatg atccaccagg gggcggagat gctcatcgag gtggcacgct acttcgccga
     2701 cctgaccacg cacgacgagg cgaccgaccg ctacgacatc gacggcgcca tgggccccga
     2761 cgagttccat gacggctatc cggggaagcc cggccagggc atgcggaaca acgcgtacac
     2821 caacgtcatg accgcgtggg tgctgcgccg cgcgatcgag accgtctcgc tgctcgaagg
     2881 gcgcaactgc cgcccgctgt ggaaccgcct cgcactccat cctgacgaga tcgaccggtg
     2941 ggaccggatc agtcgccggt tgcgcgtccc gttccacgcc gacggcgtca tcagccagtt
     3001 cgagggctat gaggacctgc cggagttcga ctggcagggc tatcgcgcac ggcacgactc
     3061 cttcgcggca ctggggatgg tcctcgccgc cgagggcgac agcacgaaca actaccggat
     3121 cgcgaaacag gcggacgtcc tgatgctgct ctacctcttc tccgccgagg aactgcgtgc
     3181 ccagctccag gagatggggt actcgttgcc gccggaggcg gtcgtgcgca cggtcgactt
     3241 ctatgcctcg cgctccacgc acgggtcgac tctcagcaac gtggcgcact cctgggtgga
     3301 ggcgcgtcgt gatcgcgaac agtcgtggca gttcctccag cgagcacttc agagcgacct
     3361 ggcggacatc cagggaagca cgacccagga gggaattcac atgggggcca tggccgggtc
     3421 tgtggacatg gtcgtgcgct gctacaccgg cctggagatc cgcaacgaca tgctctggct
     3481 ccacccggtg ctccctgcc
//
LOCUS       DASRKH010000471         3153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_38703, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000471 DASRKH010000000
VERSION     DASRKH010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38703"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VFC06_07590"
     CDS             complement(<1..430)
                     /locus_tag="VFC06_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81776.1"
                     /translation="MPSRLVSAVAGAAGYAGVWALLRVWTPGGTARWERTNYGERTVT
                     LLGGVAVAKAAVLAAATTPSPAGAVVAAFGGGAFGAIDDLGADSATSSKGLRGHLGAL
                     RHGRITTGALKILGIGATGLVSAVILTPRPRNALDVVVGTG"
     gene            complement(430..1401)
                     /locus_tag="VFC06_07595"
     CDS             complement(430..1401)
                     /locus_tag="VFC06_07595"
                     /inference="COORDINATES: protein motif:HMM:NF022824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HZL81777.1"
                     /translation="MIDFRYHLVSLISVFLALAVGIVLGAGPLREGIGDTLTGQVEVL
                     RADRDQLRADLEERTVDSNDRDAWISTMGAQVSAGTLTGRTVAIVVLPDATDDDVNAV
                     ADVLGSAGAQVTAQVRLTDAWTTSDASFRQTFAQQLAGYLNPAPAADAGPEGIMALAI
                     AQMLQGWAGADNPNPGILAELLRGGDSPFIALDTDPTAAAGSVVVIGPRPTPEPAADA
                     TPTETAAVTADVDHVQMISAFARTLPTVTVGAAEELILLLREAGVPTTTIDSIGEVPA
                     TVSVPLAVAAELGGTHGRYGSAPSANDPVPPLVSLTALQPTPEATAG"
     gene            complement(1411..2616)
                     /gene="steA"
                     /locus_tag="VFC06_07600"
     CDS             complement(1411..2616)
                     /gene="steA"
                     /locus_tag="VFC06_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HZL81778.1"
                     /translation="MVINPFQRRKDKADGGATLGLVRVDARTKNLTKRLKPGEVAVID
                     HADIDRVSAEALVACGPAAVLNAAKSTTGRYPNLGPQILLDAGIPLVDELGPGIMNLR
                     EGQRVRIDGTAVHLGDTLIAEGTLQDQPTIDRNTEVAREGLSLQLEAFAANTMEYLRK
                     ERDLLLDGVGVPEVRTRIEGRQVLIVVRGYHYKEDLQMLRSYVREYRPVLIGVDGGAD
                     AIIENGMKLDMIVGDMDSVSDSALRSGAEIVIHAYRDGRAPGLKRVEALGVDHVVFPA
                     TGTSEDVAMLLADDKGAELIVAVGTHATLVEFLDKGRSGMASTFLTRLRVGTKLVDAK
                     GVSQLSRPRIKTYQLLLLALAGLLAVVVALLATNFGQTLFGLSGAWLDDLWNLLRGLF
                     GLAPASPTS"
     gene            complement(2618..>3153)
                     /locus_tag="VFC06_07605"
     CDS             complement(2618..>3153)
                     /locus_tag="VFC06_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015771410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HZL81779.1"
                     /translation="PQPPRDGLAMKGARLGVAVEPAPLGPWGGDDVAITLVPHPGSPA
                     LPVASAASGGELSRIMLALEVALARQPGDHTFVFDEVDAGVGGSAAVEVGRRLHELAA
                     HHQVIVVTHLPQVAAFADHHVVVTKRTSATAATTIATPVVGADRERELARMLAGDESP
                     TALRHAKELLARVRVGP"
BASE COUNT          431 a         1092 c         1177 g          453 t
ORIGIN      
        1 gcccggttcc caccaccacg tcgagcgcgt tgcgggggcg gggggtcagg atgacagcgg
       61 acaccagtcc ggtggcgccg atcccgagga tcttgagggc gccggtggtg atcctgccgt
      121 gccggagggc gccgaggtgg ccccgaagtc ccttcgagct cgtcgccgag tcggcgccga
      181 ggtcgtcgat ggcgccgaag gcaccgcccc cgaaggccgc gaccacggcc ccggcggggc
      241 tgggcgtcgt cgcggcggca aggaccgccg ccttcgccac cgcgacaccg cccagcaacg
      301 tgacggtccg ctccccgtag ttcgtccgct cccaccgggc ggtgcccccc ggggtccaca
      361 cgcgcaggag tgcccacacg ccggcgtaac ccgcggcacc cgccacggcg ctcacgagcc
      421 gggacggcat cagcccgccg tcgcttccgg cgtgggctgg agggcggtga ggctgacgag
      481 cggcgggact gggtcgttgg cgctgggggc cgagccgtag cggccgtggg tgccgcccag
      541 ttcggccgcg acggcaagcg gcacggacac ggtggcgggg acctcgccga tcgagtcgat
      601 ggtggtggtc ggtactccgg cctcccgtag gaggaggatc agctcctcgg ccgcaccgac
      661 cgtcacggtc gggagggtcc tggcgaacgc ggagatcatc tgcacgtggt cgacgtcggc
      721 ggtcacggcc gcggtttcgg tgggggtcgc gtccgccgcc ggctccgggg tgggccgcgg
      781 accgatgacc acgaccgacc cggcggccgc cgtcgggtcg gtgtccagcg cgatgaacgg
      841 tgagtcgccg ccgcgcagca actcggcgag gatgccgggg ttcgggttgt cggcgcccgc
      901 ccacccctgc agcatctggg cgatcgcgag ggccatgatg ccctccggtc cggcgtccgc
      961 cgcgggcgcg gggttgaggt agccggcgag ttgctgggcg aacgtctggc ggaagctggc
     1021 gtcgctcgtg gtccacgcgt cggtcagccg gacctgcgcc gtcacctgcg cgcccgccga
     1081 cccgaggacg tcggcgacgg cgttcacgtc gtcgtcggtc gcgtccggga ggaccacgat
     1141 cgccaccgtc cgacccgtga gggtcccggc cgacacctgg gcgcccatgg tggagatcca
     1201 ggcgtcgcgg tcgttggagt cgacggtgcg ctcctccagg tccgcgcgca gctggtcgcg
     1261 gtcggcgcgg agcacctcga cctggcccgt gagcgtgtca ccgatgccct cgcgcagcgg
     1321 accggccccg aggacgattc ccaccgcgag ggccaggaac accgagatca gcgagaccag
     1381 gtggtaccgg aagtcaatca tgggttctcc tcaggaggtc ggtgaggccg gcgcgaggcc
     1441 gaagaggccg cgcagcaggt tccacaggtc gtccagccag gcgcctgaca ggccgaagag
     1501 cgtctgcccg aaattggtgg cgagcagcgc gacaaccact gcgagcagcc ccgcgagcgc
     1561 gagcagcagc agctggtagg tcttgatgcg cgggcgcgag agctgcgaga cgcccttggc
     1621 gtcgaccagc ttcgtaccca cgcgcagccg cgtcaggaag gtggaggcca tgccggagcg
     1681 gcccttgtcc aggaactcga cgagcgtggc atgggtgccg actgcgacga tcagctcggc
     1741 gcccttgtcg tccgccaaca gcatggcgac gtcctcgctg gtgccggtgg cggggaacac
     1801 cacgtggtcc actcccagcg cctcgacgcg cttgaggccg ggggcccggc cgtcgcggta
     1861 ggcgtggatg acgatctcgg ccccggacct gagcgcgctg tcggacaccg agtccatgtc
     1921 gcccacgatc atgtccagct tcatgccgtt ctcgatgatc gcgtccgcgc cgccgtccac
     1981 accgatcagg acgggacggt actcgcggac ataggacctg agcatctgca ggtcctcctt
     2041 gtagtggtag ccccgcacga cgatgaggac ctggcgcccc tcgatcctcg tgcggacctc
     2101 ggggacaccg acgccgtcga ggaggaggtc ccgttccttg cggaggtact ccatcgtgtt
     2161 cgcggcgaac gcctccagct ggagcgagag gccctcgcgc gccacctcgg tgttccggtc
     2221 gatcgtgggc tggtcctgga gcgtgccctc ggcgatcagc gtgtcgccga gatggacggc
     2281 cgtgccgtcg atgcggaccc gctggccctc gcgcaggttc atgatgccgg gcccgagttc
     2341 gtcgacgagg gggatcccgg cgtccagcag gatctgcggc ccgaggttcg ggtaccgacc
     2401 ggtggtggac ttggcggcat tcagcacagc ggccggcccg caggcgacga gcgcctccgc
     2461 ggagaccctg tcgatgtccg cgtggtcgat gacggcgacc tccccgggtt tcagccgctt
     2521 ggtgaggttc ttcgtgcgcg cgtcgacacg caccaggcca agcgttgccc caccgtcggc
     2581 cttgtccttc cggcgctgaa atggattgat caccatgtca tggtcccacg cgcacgcgcg
     2641 cgaggagctc cttcgcgtgc cggagtgcgg tgggtgattc atcacctgcg agcatgcgcg
     2701 ccagctcgcg ctcgcggtcg gcgcccacga caggggtggc gattgtggtg gcagccgtgg
     2761 ccgatgtccg cttcgtcacg acgacgtggt ggtcggcaaa tgcggccacc tgcgggaggt
     2821 gggtgacgac aatgacctgg tggtgcgcgg cgagttcgtg cagccggcgc cccacctcga
     2881 cggcggcgct gcccccgacc ccggcgtcca cctcgtcgaa gacgaaggtg tggtcccccg
     2941 gctgccgcgc gagcgccacc tcgagggcca gcatgatgcg ggagagttca cccccggagg
     3001 ccgccgacgc gaccggcagt gcgggcgagc cggggtgcgg caccagggtg atggcgacgt
     3061 cgtcgccgcc ccacgggccg agcggtgccg gctccacagc caccccgaga cgggctccct
     3121 tcatggcgag cccgtctcgg ggtggctgtg gag
//
LOCUS       DASRKH010000472         2209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_4542, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000472 DASRKH010000000
VERSION     DASRKH010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4542"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VFC06_07610"
     CDS             complement(<1..258)
                     /locus_tag="VFC06_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HZL81780.1"
                     /translation="MIDLRSDTVTRPTDAMRAAMLRAPVGDDVYGEDPSVVELERRTA
                     ALLGHEAGLFCASGSMCNLLGIWLLVRPGQEVLCDAGAHIAR"
     gene            413..763
                     /locus_tag="VFC06_07615"
     CDS             413..763
                     /locus_tag="VFC06_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015771580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein RbpA"
                     /protein_id="HZL81781.1"
                     /translation="MADRSLRGMSIGSKSMETEENVEFAPRQMARYDCPNGHVIELPF
                     SVEADVPAVWECRCGEEAILKDHIKPEPLKPVKHVRTHWDMLLERRSIEELEVLLSER
                     LELLRSGELRRRSA"
     gene            complement(800..1534)
                     /locus_tag="VFC06_07620"
     CDS             complement(800..1534)
                     /locus_tag="VFC06_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HZL81782.1"
                     /translation="MKTLVIIPTYNERENIRDIVTRVRREDVDVLVADDASPDGTGAI
                     ADELAAADPRVFVKHRAGKEGLGRAYIDAFGWGLARDYTHLVEMDADGSHRPEQLPLL
                     LERAARADLPDLVIGSRWVPGGEVVNWPWSRMFLSRAGNLYVRLWLGLPAADATAGFR
                     VYRSEILRRIDFDTVGSSGYYFQVEMTRQAALAGGVVVEVPISFIERERGTSKMSNAI
                     VREAMVRTTLLGARHRWQQVRAALRR"
     gene            complement(1531..>2209)
                     /locus_tag="VFC06_07625"
     CDS             complement(1531..>2209)
                     /locus_tag="VFC06_07625"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HZL81783.1"
                     /translation="AAQDAAGVALLFGTQRYTEEYRYNEYVAWLDGAAIGAYTKQHPV
                     PFGEYIPFRDFFRTLSSAVDLVSVDMAQGQDPGVLEIPVERLGRTVTFGVGICFEVAY
                     HELLRESVAHGAELLVIPTNNASFGFTQEATQQLGITRFRAVEHGRSTVQVSTVGVSA
                     YFLPDGSTVARSTDELYTEWVGLETLPLRTSTTVSDRLGEWPRNVVWIVALAWIAAAL
                     VSRRRRT"
BASE COUNT          353 a          808 c          727 g          321 t
ORIGIN      
        1 ccgggcgatg tgtgccccgg cgtcgcacag gacctcctgg ccgggacgga cgagcaacca
       61 gatgcccagc aggttgcaca tgctcccgct ggcgcagaac agcccggcct cgtggcccag
      121 caacgcggcc gtacggcgtt ccagctcgac gacggacggg tcctccccgt agacgtcgtc
      181 acccacgggc gcccgcagca tcgcggcgcg catggcgtcg gtggggcggg tcacggtgtc
      241 gctgcgcagg tcgatcacct ccccagcgta ggacgaccgc ccgggcgccg aggccgtgac
      301 ccggccgtgt ggcaccgccg ggaacaaaag caccccccga ggcgttcaac acatcgggaa
      361 ggtccacgag gccgtattcg cactcgtcca caagacacca aggggttcac gcatggcaga
      421 ccgctcactt cgaggcatga gtatcggctc caagtccatg gagaccgagg agaacgtcga
      481 gttcgccccg cgccagatgg cgcgctacga ctgcccgaac gggcacgtca tcgagctgcc
      541 cttctccgtc gaggccgacg tgcccgcggt gtgggaatgc cgctgcggcg aggaggcaat
      601 cctcaaggac cacatcaagc ccgagcccct gaagccagtg aagcacgtcc gcacccactg
      661 ggacatgctg ctggagcgcc gttcgatcga ggagctcgag gtgctgctca gtgaacggct
      721 cgaactcctg cgttccggcg agctccgccg tcgctcggcc tgaagccgct ccccccgcat
      781 catcgggccg ctggccgggt cagcgccgca acgcggcgcg cacctgctgc cagcggtggc
      841 gcgcgcccag cagcgtggtg cgcaccatcg cctcccggac gatcgcgttg ctcatctttg
      901 acgtgccgcg ctcccgctcg atgaaactga tgggcacctc caccaccact cccccggcca
      961 gcgccgcctg ccgtgtcatc tccacctgga agtagtaccc cgaggagccg accgtgtcga
     1021 agtcgatccg ccgaaggatc tcgctgcggt agacccggaa cccggccgtg gcgtccgcgg
     1081 cgggcaggcc gagccacagc cgcacgtaga ggttccccgc gcgggacagg aacatccggg
     1141 accacggcca gttgaccacc tctcccccgg gcacccaccg ggagccgatc accaggtcgg
     1201 gcaggtccgc gcgggccgcc cgctccagca ggagggggag ctgttccggg cggtgcgaac
     1261 cgtcggcgtc catttccacg aggtgcgtgt agtcccttgc gagaccccac ccgaacgcgt
     1321 cgatgtaggc cctgcccaag ccctccttgc ccgcgcggtg cttcacgaac accctcgggt
     1381 ccgcggcggc cagttcatcc gcgatcgcgc cggtgccgtc cgggctggcg tcgtcggcca
     1441 cgagcacgtc gacgtcctcg cggcgcacgc gcgtcacgat gtcgcggatg ttctcgcgct
     1501 cgttgtaggt ggggatgatg acgagcgtct tcatgtcctt ctcctgcggc tcacgagcgc
     1561 tgccgcgatc cacgccagcg cgacgatcca gaccacgtta cggggccact cccccagccg
     1621 gtcggaaaca gttgtcgatg tccggagcgg gagggtctcg agccccaccc actcggtgta
     1681 cagctcgtcc gtggaccgcg ccacggtgga gccgtcgggg aggaagtacg cactgacgcc
     1741 caccgtcgag acctggacgg tgctgcgccc gtgctccacg gcgcggaacc gcgtgatgcc
     1801 gagctgctgc gtcgcctcct gcgtgaaccc gaacgacgcg ttgttcgtgg ggatcaccag
     1861 cagttccgca ccatgggcaa cgctttcgcg caggagttcg tggtacgcca cctcgaagca
     1921 gatgcccacc ccgaacgtca cggtgcggcc gagccgttcc acggggatct ccagcacccc
     1981 cggatcctgg ccctgtgcca tgtccaccga gaccaggtcc accgcggacg agagcgtccg
     2041 gaagaagtcc cggaagggga tgtactcccc gaacggcacg gggtgctgct tcgtgtacgc
     2101 gccgatggcc gcgccgtcga gccaggcgac gtactcgttg tagcggtact cctcggtgta
     2161 ccgctgcgtg ccgaacagca gcgcgacgcc cgccgcgtcc tgggcggcc
//
LOCUS       DASRKH010000473         2257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_57144, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000473 DASRKH010000000
VERSION     DASRKH010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57144"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1045
                     /locus_tag="VFC06_07630"
     CDS             <1..1045
                     /locus_tag="VFC06_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZL81784.1"
                     /translation="VWADRLNRKMLIIGADAMIATATLALAMLMLGGADDLWLIYAAL
                     VIRSVGAGVQMPAVGALLPQIVPTEKLMRVNGINGTIQSAMMLVAPAAAAAVYASSSI
                     VAVFFVDIVTAVIGIGLLSLLAVPRLVRPELTGDVHAAYFDDLLGGLRYAVSHTFVRW
                     LLALYAVVFVLIVAPSYLTPLMIVRTFGAEVWKLTVNEVAFSVGMILGGALLATWGGL
                     KNRVTMIVASTLAFGGLSVGLGLSTHLVVFFGFMFLVGLAVPFFSTTSMTVLQETVEP
                     EMQGRVFGFVGIVMALALPVGMAVFGPLADRFTVEAVLIASGVLTFLVVGAAVGLPPG
                     RRAVEAAREHSLS"
     gene            1060..1275
                     /locus_tag="VFC06_07635"
     CDS             1060..1275
                     /locus_tag="VFC06_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81785.1"
                     /translation="MTGNGLGGTGWQVPPHELARLRTSTVRASSAASLALLIAFCDTQ
                     LLFGIAIDVALIVLAVMRPGWTVWVSG"
     gene            1396..>2257
                     /locus_tag="VFC06_07640"
     CDS             1396..>2257
                     /locus_tag="VFC06_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018775445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HZL81786.1"
                     /translation="MIVFTDRVDAGRQLATKLEQLRGQDAVVLGLPRGGVPVAFEVAE
                     ALDLPLDVIVVRKLGVPYQPELAMGAIGEGGARVLDSRVLAVTGVSEQDLAVIESRER
                     AELDSRVARLRRGRDPVDLSGRIAVIVDDGIATGSTARVACEVARHLGATRVIVAVPV
                     APVEAARGLPGADEVVCVSAPREFMAVGYHYRDFSPTSDEEVIVLLDAAARRMQGVPT
                     FGDAPDCDVDVEIPTGAVALQGHLHLPEPVTGVVLFAHGSGSSRHSPRNRFVASVLQR
                     AGLGTLLLDLL"
BASE COUNT          275 a          794 c          790 g          398 t
ORIGIN      
        1 ggtgtgggcc gaccggctga accggaagat gctcatcatc ggcgccgacg ccatgatcgc
       61 gacggccaca cttgctctcg ccatgctcat gctcggcggg gccgacgacc tgtggctcat
      121 ctacgccgcg ctggtgatcc ggtcggtggg cgccggcgtc cagatgccgg cggtcggtgc
      181 gctcctgccg cagatcgtgc cgaccgagaa gctcatgcgc gtcaacggca tcaacggcac
      241 catccagtcc gccatgatgc tcgtcgcacc ggcggccgca gctgccgtct acgcgagctc
      301 gtcgatcgtt gcggtgttct tcgtcgacat cgtcacggcg gtcatcggca tcgggctgct
      361 gagcctcctc gccgtcccgc ggctggtccg ccccgagctg acgggcgacg tacatgccgc
      421 gtacttcgac gacctgctcg gcggactgcg ctacgccgtg tcgcacacgt tcgtgcgctg
      481 gctgctcgcg ctctacgcgg tcgtgttcgt gctcatcgtc gctccgtcct acctgacacc
      541 gctcatgatc gtgcggacct tcggcgccga ggtgtggaag ctcaccgtca acgaggtcgc
      601 gttcagcgtc ggcatgatcc tcggcggcgc actgctcgcg acctggggcg ggctcaagaa
      661 ccgcgtcacg atgatcgtgg cctcgacgct ggccttcggc gggctctcgg tcgggctcgg
      721 cctgtcgacg cacctcgtcg tcttcttcgg cttcatgttc ctcgtcggcc tggccgtgcc
      781 gttcttctcg acgacctcga tgacggtcct gcaggagacg gtcgagcccg agatgcaggg
      841 acgggtgttc gggttcgtcg gcatcgtcat ggcgctcgcc ctgccggtgg gcatggcggt
      901 cttcggtccg ctcgctgacc ggttcacggt cgaggccgtg ctgatcgcct ccggcgtgct
      961 cacgttcctc gttgtcggcg ctgccgtcgg cctgccgccg gggcgtcgtg ccgtggaggc
     1021 tgcccgcgag cactccctgt cctgaccacc tacgagtcct tgaccgggaa cggcttgggc
     1081 ggcaccggat ggcaggtgcc gccccacgag ctcgccagac tcaggacgtc gacggtgcgt
     1141 gcctcgtcgg cggcctcgct cgcgctgctg atcgccttct gcgacaccca gctgctcttc
     1201 ggcatcgcga tcgacgtggc attgatcgtc ctcgcggtca tgcgccccgg gtggacggta
     1261 tgggtcagcg gctgatccct tgcgcggacg cagctcgcag ctgacgagct ccggccctgc
     1321 gcaccggacg ctgcggctgc ggcggaggcc agccaggagg atggttgtca ggtaggtgag
     1381 agaggggtca ccgccatgat cgtcttcaca gaccgggtcg acgccggacg gcagctggcg
     1441 acgaagctcg agcagctgcg cggtcaggac gcggtggtgc tgggcctgcc acgaggcggc
     1501 gtgcccgtgg cgttcgaggt ggcagaggcg ctcgacctgc cgctcgacgt catcgtggtc
     1561 cgcaagctcg gcgtccccta ccagcccgag ctcgccatgg gggccatcgg cgagggcggc
     1621 gcgcgcgtgc tcgactcgag agtgctggcg gtcaccggcg tctccgagca ggacctggcc
     1681 gtgatcgaga gtcgcgaacg ggctgagctc gactcgcgcg tcgcgcgcct gcgcaggggg
     1741 cgagatcccg tcgacctctc cgggcgcatc gcggtcatcg tcgacgacgg catcgccacg
     1801 ggttcgacgg cgcgcgtcgc gtgcgaggtg gcacgtcatc tcggcgccac gcgagtcatc
     1861 gtggcggtgc ctgtcgcccc ggtcgaggcc gcccgtggcc tgcccggagc cgacgaggtc
     1921 gtgtgcgtct cggcgccacg ggagttcatg gcggtcggct accactaccg cgacttctcc
     1981 ccgacgagcg acgaggaggt gatcgtcctg ctcgacgccg cggcgcggcg catgcagggc
     2041 gtgccgacgt tcggcgacgc cccggactgc gacgtcgacg tcgagatccc caccggtgcg
     2101 gtcgcgctcc agggtcacct tcaccttccg gaacccgtga ccggcgtcgt gctgttcgct
     2161 cacggcagcg gcagcagccg ccacagcccg cgcaaccggt tcgtcgcctc ggtgctccag
     2221 cgagccggtc tgggcacgct cctgctcgac ctgctca
//
LOCUS       DASRKH010000474         3226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_7450, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000474 DASRKH010000000
VERSION     DASRKH010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7450"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..699
                     /locus_tag="VFC06_07645"
     CDS             73..699
                     /locus_tag="VFC06_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81787.1"
                     /translation="MKGTSGHAQDWRQWLGVARRRITSVAFVARVRNAKGDEQDDGVP
                     QRLSRAERWVCMTLRSRLVPSLIVATAYMLAASGCAAPGDAAGAPSTAIPADAGGAAG
                     IQLAQPSGAPEPGAVAKRRAPEAVGAVIGITMGEDSVQVQFVPDAGYEYYAGTTFDIP
                     FSAVAAIDGETAVVLGDLVVGDRLNVWTGACRESYPVQCDIEAVTAAE"
     gene            731..2404
                     /gene="argS"
                     /locus_tag="VFC06_07650"
     CDS             731..2404
                     /gene="argS"
                     /locus_tag="VFC06_07650"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012398030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HZL81788.1"
                     /translation="MTPEELSATIAVCIEESIAAGEIALTNEDVVEVRVERPKVREHG
                     DWATNVAMQLAKKAGTNPRAFAEIVAARLLTVDGIDAADVAGPGFLNIRLGAGAAGEL
                     ARSIVEEGEDYGRGESFAGVHVNVEFVSANPTGPIHLGGTRWAAVGDSLSRLLEFHGA
                     TVTREYYFNDHGVQIDRFAASLLAAIKGEDSPEDGYGGAYILETADQVLQDAMEAGER
                     NPLELPRDQALEFFRVRGVHLMFDQIKQSLHDFGVDFDVYFHEDSLHKSGKVEHALNH
                     LKKTGALYESEGAWWLRTTEQGDEKDRVVIKSDGQAAYIAGDIAYIRDKHERGAQLCI
                     YLLGADHHGYIARLKAGAAALGYNPDSVEVIIGQMVNLVKDGAPVRMSKRAGTVVTME
                     DLVDVVGVDAARYALTRSSADATLDVDLDVLASATNANPVFYVQYAHARTAGVARNAA
                     EYGITRHDGFDASLLTHESEAALLGVLGEFPRIVAQAVDLREQHRVARYLERLASAFN
                     QWYDRTRVTPQGDEEITDLNRTRLWLNDATGQVLRNGLGLLGVGAPERM"
     gene            2407..3180
                     /locus_tag="VFC06_07655"
     CDS             2407..3180
                     /locus_tag="VFC06_07655"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81789.1"
                     /translation="MPVWSQTVARGDDGALTVGGCDVRALAAEHGTPLYVVDEADFRA
                     RARGFRDHFAAAFARHGARCDVFYASKALLSSQIARWVLDEGLGIDVASGGELELALR
                     GGVPGDRITLHGNNKSDAELSRALEVGVGRIVADSTTEIEVLGSLAAEAGAVAPVLVR
                     VTTGVHAGGHEYIATSHEDQKFGLSLATGAALAALHAVHSSPHLELRGIHTHIGSQIL
                     SIGAFKDSAERMLRLRAEFAAATGFEMPEVDLGGGYAIA"
BASE COUNT          514 a         1068 c         1132 g          512 t
ORIGIN      
        1 cgacctacgg taccggaaac cggcgctcta tcctctgagc tacgggggca tcgcggggct
       61 cggccccgca tcatgaaggg taccagcggg cacgcacaag attggcggca gtggttgggg
      121 gtggcacggc gccgcatcac gagtgtggcg ttcgtcgcgc gggtgcgtaa tgccaaagga
      181 gatgaacagg acgacggggt accccaacgg ttgtctcgcg cggagcgttg ggtatgcatg
      241 acactgcgct ctcgactcgt cccgtccctc atcgtcgcaa ctgcctacat gcttgccgcc
      301 agcgggtgtg cggcgcctgg tgacgccgcg ggcgcccctt ccacggcgat tcccgcagat
      361 gcgggcgggg cggctggcat ccagctcgcg cagccaagcg gtgcccccga gcccggggcg
      421 gtggccaagc ggcgcgctcc cgaggccgtg ggcgcggtca tcggcatcac gatgggcgag
      481 gactccgttc aggtgcagtt tgtgcccgat gccggctacg agtactacgc ggggacgacg
      541 ttcgacatcc ccttctcggc ggtggccgcg atcgacggcg agactgcggt ggtgctcggc
      601 gacctggtgg tgggtgacag gctcaacgtg tggacgggcg cgtgccgcga gtcctatccg
      661 gtgcagtgcg acatcgaggc ggtcaccgca gccgagtagt cgcgcggcgg cgcgccgata
      721 gactcgtcgt gtgactcctg aagaactctc cgcgaccatc gccgtctgca tcgaggaaag
      781 catcgccgca ggcgagattg ccctcaccaa cgaggacgtt gtcgaggtgc gcgtcgagcg
      841 acccaaggtg agggagcacg gcgactgggc caccaacgtg gcgatgcagt tggccaagaa
      901 ggcgggcacg aacccccgtg cgttcgccga gatcgtggcg gcgcggctgc tcacggtcga
      961 cggcatcgac gcggccgacg tcgctggccc gggctttctc aacatccggc tcggcgcggg
     1021 agccgctggc gaactggcga ggtcgattgt cgaggaaggc gaggattacg gccgcggtga
     1081 gtccttcgcg ggcgttcacg tcaacgtcga gttcgtctcg gccaacccca ccggccccat
     1141 ccacttgggc ggcacccgct gggccgccgt cggcgactcg ctcagccgct tgctggagtt
     1201 tcacggcgcg actgtgaccc gcgagtacta cttcaacgac cacggcgtgc agatcgaccg
     1261 ctttgcggcg tccctgcttg cggccatcaa gggcgaggat tcccccgagg acggctacgg
     1321 cggcgcgtac atcttggaga ccgccgatca ggtgctgcaa gacgccatgg aggccggcga
     1381 gaggaatccg cttgagcttc ctcgcgacca ggcgctggag ttcttccggg tgcgcggcgt
     1441 gcacctcatg tttgatcaga tcaaacagtc gctgcacgac ttcggcgtgg acttcgacgt
     1501 gtacttccat gaggactcgc tgcacaagtc cggcaaggtc gagcacgcgc tgaaccacct
     1561 caagaagacc ggcgcgctgt acgagtccga gggtgcctgg tggctgcgta ccaccgagca
     1621 gggggacgaa aaggatcgcg tggtcatcaa gtccgacggt caggcggcct acatcgcggg
     1681 cgacatcgcg tacatccgcg acaagcacga acgcggcgcg cagctgtgca tctacctctt
     1741 gggcgccgac caccacggct acatcgcacg cctcaaggcg ggcgctgccg cgctggggta
     1801 caaccccgac tcggtcgagg tcatcatcgg gcagatggtg aatctggtga aggacggcgc
     1861 gcccgtgcgg atgtccaagc gagccggcac cgtggtcacg atggaggacc tcgtggacgt
     1921 ggtcggcgtg gacgccgccc gctacgcact cacacgttcc agcgccgatg ccacgctcga
     1981 cgtcgatctt gacgtgctcg cgtcggccac caacgccaat cccgtgttct acgtccagta
     2041 cgcccacgcg cgcactgcgg gcgtggcgcg caacgccgcc gaatacggaa ttaccaggca
     2101 tgatggtttt gatgcatccc tgctgacgca cgagtcggag gcggcgctgc ttggagtgct
     2161 gggcgagttc ccgcgcatcg tcgcccaggc cgttgatctt cgggagcagc accgcgtcgc
     2221 gcgctacctc gagcgactcg cctccgcctt caaccagtgg tacgaccgca cccgcgtcac
     2281 cccgcagggc gacgaggaga tcacggatct caaccgcacg cgcctgtggc tcaacgacgc
     2341 cacgggtcag gtgttacgca atgggctcgg gctgttgggc gtcggcgcac ccgaaaggat
     2401 gtagcgatgc cggtgtggtc tcagactgtg gcgcgcggcg acgacggcgc gctcaccgtg
     2461 ggcggctgcg acgtgcgcgc gctcgcggcg gagcacggca cgccactgta cgtggtcgac
     2521 gaggccgact tcagggcccg cgcgcgtggc ttccgcgatc acttcgccgc ggccttcgca
     2581 cggcacggcg cccggtgcga cgtgttctac gcgtcgaagg cactgctcag ctcgcagatt
     2641 gcgcgctggg tgctggacga gggtctcggc atcgacgtgg ccagcggcgg cgagctcgag
     2701 ctcgcgctgc gcgggggagt tcccggcgat cgcatcacgc tgcacggcaa caacaagtcc
     2761 gacgccgaac tgagccgcgc gctcgaggtc ggcgtggggc gcatcgtggc ggactcgacg
     2821 accgagatcg aggtgctcgg ctcacttgcg gcggaggccg gggccgtcgc tcccgtgctg
     2881 gtgcgcgtca ccaccggagt gcacgctggc ggtcacgaat acatcgccac gagtcacgag
     2941 gaccagaagt tcgggctgtc tttggctacc ggggccgcgc tcgcggctct ccacgcggtg
     3001 cactcctcgc cccacctgga gttgcgcgga atccacacgc acatcggcag ccagatcctg
     3061 tcgatcgggg ccttcaagga ctcggccgag cggatgctgc gcctgcgcgc cgagttcgcc
     3121 gcggcgaccg gattcgagat gcccgaggtg gacctgggtg gcggctacgc gatcgcgtag
     3181 ccgccaccca ggtccacctc gggcatctcg aatccggtcg ccgcgg
//
LOCUS       DASRKH010000475         2972 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_43249, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000475 DASRKH010000000
VERSION     DASRKH010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43249"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1139
                     /gene="argJ"
                     /locus_tag="VFC06_07660"
     CDS             <1..1139
                     /gene="argJ"
                     /locus_tag="VFC06_07660"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HZL81790.1"
                     /translation="VDDQGEVGPAGFRAAGVTAGLKPSGRPDLALVVNDGPLQVAAGV
                     FTSNRVVAAPVVWSRQAVSDGLASAVVLNSGGANACTGPDGFADTHRTAEHVATVLGV
                     SAGDVLVCSTGLIGERLPMGTLLAGVDAAAVALSPTGGPDAAVAIMTTDTVAKTVLVE
                     GDGWSVGGMAKGAGMLAPGLATMLVVLTTDAVVTAAQADATLRAATRTTFDRVDSDGC
                     MSTNDTVLLLASGASGVSVSDAQLAAAVTQAAAQLARRLVADAEGASHDIAVTVTNAE
                     TEDAAVAVARAVTRSNLFKAAVFGNDPNWGRVLAAVGTVPVEVAAFDASTLDVAINGV
                     MVCRAGGVGDDRGLVDLHAGDATATVWTNDLTHDYVHENSAYST"
     gene            1136..2128
                     /gene="argB"
                     /locus_tag="VFC06_07665"
     CDS             1136..2128
                     /gene="argB"
                     /locus_tag="VFC06_07665"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HZL81791.1"
                     /translation="MTDESQGFVFNTRTDLRADQKAEVLIEALPWLQRFSGALVVVKY
                     GGNAMVDENLKRAFAQDMVFLRQVGLRPVVVHGGGPQINTMLDRLGIASEFRGGLRVT
                     TPETMDVVRMVLTGQVSRELVGLINAHGPHAVGLSGEDGGLLRARRRSATVGGEQVDI
                     GLVGDVVEVNPSAVLDLLDAGRIPVVSTVAPDIDDPTQVLNVNADSAAAALAIALGAR
                     KLIVLTDVEGLYRDWPDTSSLVRRIRSSELAELLPHLDSGMRPKMEACRRAVDGGVGR
                     AHVIDGRLPHSILVEVFTSDGIGTMVLPDDEPVPSPFTGQMPVVDEHPTVGGHA"
     gene            2125..>2972
                     /locus_tag="VFC06_07670"
     CDS             2125..>2972
                     /locus_tag="VFC06_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZL81792.1"
                     /translation="MSDDELVPTASSLVAPDSGAHWTQRYSAALMNTFGPPQRVLVRG
                     DGAYVWDADGTRYLDLLSGIAVNSLGHAHPTLTAAVSAQLGTLGHVSNFFASPVQIAL
                     AERLLGLAGAPAGSRVFFANSGTEANEALFKMARRTGRRRIIALEGSFHGRTMGALAM
                     TSKAAYREPFEPLPGGVEFVPFGDSAALEAVFADGGRDVAAVVVETIQGEAGVRPLPP
                     GYLALARELTGKAGALLAIDEVQTGMGRTGAWFAHHHPYLGGGVVPDVVTLAKGLGGG
                     IPIGAL"
BASE COUNT          386 a         1096 c         1069 g          421 t
ORIGIN      
        1 tcgttgacga ccagggcgag gtcgggccgg ccgggttccg cgccgcaggg gtcaccgcgg
       61 gcctcaagcc gtccggccgg cccgacctcg ccctggtcgt caacgacggg ccgctccagg
      121 tcgccgcggg cgtgttcacg agcaaccggg tcgtcgccgc gcccgtcgtg tggtcgcgcc
      181 aggccgtctc ggacggcctc gcctccgccg tggtgctgaa ctccggcgga gcgaacgcgt
      241 gcaccgggcc cgacggcttc gcggacacgc accggaccgc cgagcacgtc gcgacggtgc
      301 tcggggtctc ggcgggcgac gtgctcgtgt gctcgaccgg cctgatcggc gagcgcctgc
      361 cgatgggcac gctgctcgcc ggcgtcgacg ccgcggccgt ggccctgagc cccaccggcg
      421 gcccggacgc ggccgtcgcg atcatgacga ccgacacggt ggccaagacc gtgctcgtcg
      481 agggggacgg ctggtcggtc ggcggcatgg ccaagggcgc cggcatgctc gcgcccggcc
      541 tggccacgat gctcgtcgtg ctcaccaccg acgcggtcgt gacggccgcg caggccgacg
      601 ccacgctgcg ggccgcgacc cgcacgacgt tcgaccgggt cgactccgac ggctgcatgt
      661 ccaccaacga cacggtgctg ctgctcgcct ccggcgcatc gggcgtgtcg gtgtccgacg
      721 cgcagctggc cgccgcggtc acgcaggcgg ccgcgcagct ggcccgccgg ctcgtcgcgg
      781 atgccgaggg cgcgagccat gacatcgccg tgaccgtcac gaacgccgag acggaggacg
      841 cagcggtcgc cgtcgcgcgc gccgtgaccc ggtccaacct gttcaaggcc gcggtcttcg
      901 gcaacgaccc caactggggc cgcgtgctcg ccgcggtcgg cacggtcccc gtcgaggtcg
      961 ccgcgttcga cgcgagcacg ctcgacgtcg cgatcaacgg cgtcatggtg tgccgagccg
     1021 gcggcgtcgg tgacgaccgc gggctcgtcg acctgcacgc gggcgacgcg accgccacgg
     1081 tgtggaccaa cgacctcacg cacgactacg tccacgagaa cagcgcgtac tccacatgac
     1141 cgacgagagc cagggcttcg tgttcaacac ccgcaccgac ctgcgcgccg accagaaggc
     1201 cgaggtcctc atcgaagccc tgccctggct gcagcgcttc tccggcgcgc tcgtcgtcgt
     1261 gaagtacggc gggaacgcga tggtcgacga gaacctcaag cgcgcgttcg cgcaggacat
     1321 ggtcttcctt cggcaggtcg ggctacgccc cgtcgtcgtg cacgggggcg ggccgcagat
     1381 caacacgatg ctcgaccggc tcggcatcgc gagcgagttc cgcggcgggc tgcgcgtcac
     1441 gacgcccgag acgatggacg tcgtccggat ggtcctgacg ggccaggtct cgcgcgagct
     1501 cgtcgggctc atcaacgcgc acggcccgca cgcggtcggc ctgtcgggcg aggacggcgg
     1561 gctgctgcgc gctcgtcggc gctccgcgac agtcggcggc gagcaggtcg acatcggcct
     1621 cgtcggtgac gtcgtcgagg tcaacccgtc ggccgtgctc gacctgctcg acgcgggccg
     1681 catccccgtc gtgtcgacgg tggcccctga catcgacgac ccgacccagg tgctgaacgt
     1741 caacgccgac agtgcggcgg ctgcgctcgc gatcgcgctc ggtgcccgca agctcatcgt
     1801 cctgaccgac gtcgagggtc tctaccgcga ctggccggac acgagctcgc tcgtccgccg
     1861 gatccgctcg agcgagctcg ccgagctgct gccgcacctc gactcgggga tgcgcccgaa
     1921 gatggaggcg tgccggcgcg cagtcgacgg cggcgtcggg cgtgcacacg tcatcgacgg
     1981 tcgcctgccg cactcgatcc tcgtcgaggt cttcacctcg gacggcatcg gcacgatggt
     2041 gctgcccgac gacgagccgg ttccgtcccc gttcaccggc cagatgcccg tcgtcgacga
     2101 gcaccccact gtcggagggc acgcatgagc gacgacgagc tcgttccgac cgcgtcgtcc
     2161 ctcgtcgccc cggactcggg cgcgcactgg acccagcggt actccgcggc actcatgaac
     2221 acgttcgggc cgccccagcg cgtgctcgtg cgcggtgacg gtgcgtacgt gtgggatgcc
     2281 gacggcacgc ggtacctcga tctgctgagc gggatcgcgg tcaactcgct cgggcatgcg
     2341 cacccgaccc tgaccgccgc ggtgtccgca cagctcggca cgctcggtca cgtctcgaac
     2401 ttcttcgcct ccccggtgca gatcgcgctc gccgagcggc tgctcgggct tgccggcgca
     2461 cccgcaggtt cgcgcgtgtt cttcgccaac tcgggcacgg aggcgaacga ggcgctgttc
     2521 aagatggccc gtcgcaccgg acggcggcgg atcatcgcgc tcgaggggtc gttccacgga
     2581 cgcacgatgg gcgcgctcgc gatgacgtcc aaggccgcct accgcgagcc cttcgagccg
     2641 ctcccgggtg gcgtcgagtt cgttccgttc ggtgactcgg cggcgctcga ggcggtgttc
     2701 gccgacggtg gccgggacgt ggcggccgtc gtcgtcgaga cgatccaggg cgaggccggt
     2761 gtgcgcccgc tgccccccgg ctacctcgcc ctcgcgcgtg agctgacggg caaggcggga
     2821 gcgctgctgg ccatcgacga ggtgcagacc ggcatgggcc gcaccggcgc gtggttcgcg
     2881 caccaccacc cgtacctggg tggcggtgtc gtgcccgacg tcgtgacgct cgcgaagggt
     2941 ctgggcggcg gcatcccgat cggcgcgctc at
//
LOCUS       DASRKH010000476         2949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_4621, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000476 DASRKH010000000
VERSION     DASRKH010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4621"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1507
                     /gene="infB"
                     /locus_tag="VFC06_07675"
     CDS             <1..1507
                     /gene="infB"
                     /locus_tag="VFC06_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HZL81793.1"
                     /translation="TVMGHVDHGKTRLLDAIRKSDVVSGEAGGITQHIGAYQVRRDHE
                     GDTRAITFIDTPGHEAFTAMRARGAQVTDVAILVVAADDGVMPQTVEALNHAQAANVP
                     IVVAINKIDKEGANADKIRQQLTEYNLVAEEYGGETIFVEVSATQGKNIEKLLDAVLL
                     TADAGLDLRANPNKDARGVAVEAHLDKGRGPVATVLVQSGTLRVGDAIVAGTAYGRVR
                     AMFNEHDEPVAEALPARPVMVIGLTSVPRAGDTFLAAEDDRTARQIAEKREAAERAAQ
                     LAKRRKRISLEDFTQAIEDGKVNTLNLIIKGDVSGAVEALEDALLAIDVGDSVDLRVI
                     HRGVGAVTQNDVNLATVDSAVIIGFNVRPAERVQELAEREGVDMRFYSVIYRALEDVE
                     ASLTGMLKPEYEEKQIGSAEIMEIFRSSKFGNIAGCLVRNGVIKRNAKARVVRSGNVI
                     GEPTIDTLRRFKDDATEVKDGFECGIGLGKFNDLQVGDMIETFEMVEIPRS"
     gene            1507..1947
                     /gene="rbfA"
                     /locus_tag="VFC06_07680"
     CDS             1507..1947
                     /gene="rbfA"
                     /locus_tag="VFC06_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HZL81794.1"
                     /translation="MADSPRALRLAGTIKKIVARALENVIKDPRLGFVTVTDVRVTGD
                     LQQASVFYTVYGGDEDRANTAAALESAKGRLRSMVGGELGIRLTPSLEFFLDAVPETA
                     ASLEKSLHEAALRDAELAKLREGAAFAGEADPYRHPSDEDDSED"
     gene            1952..2908
                     /gene="truB"
                     /locus_tag="VFC06_07685"
     CDS             1952..2908
                     /gene="truB"
                     /locus_tag="VFC06_07685"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HZL81795.1"
                     /translation="MARRIDEPTPGLLLVDKPAGWTSHDVVARSRRILNTRKVGHAGT
                     LDPMATGLLVLAVGRATRLLTYVTGHDKSYDATIRLGQSTITDDAEGEVTSTADASSI
                     SDDALAAAIADLTGDISQVPSSVSAIKVNGERAYARVRAGEEVALAARPVTVSRFEVT
                     AVRREGTMIDVDATIDVSSGTYIRALARDLGAALGVGGHLTALRRTRIGRFDVASART
                     VDALAALAGSGADIPLIPLDEAAKAALPVLALTEQEAVSLGFGKPLRERADELTKPEH
                     DDDFTHDAAAVAPDGRVIALVRRVKGAAKPVIVFLPAGAARD"
BASE COUNT          509 a          981 c          991 g          468 t
ORIGIN      
        1 caccgtcatg ggtcacgtcg accacggtaa gacccgtctg ctggacgcca tccgcaagtc
       61 cgacgtcgtg tcgggcgaag cgggcggtat cacccagcac atcggtgctt accaggtgcg
      121 ccgcgaccac gagggcgaca ctcgcgccat caccttcatc gacacgccgg gtcacgaggc
      181 gttcaccgcc atgcgtgcac gtggtgcaca ggtgaccgac gtcgccatcc tcgtggtggc
      241 cgccgacgac ggcgtcatgc cgcagacggt ggaagccttg aaccacgcgc aagcggccaa
      301 cgtgccgatc gtggtggcga tcaacaagat cgacaaggaa ggcgccaacg ccgacaagat
      361 tcgccagcag ctcaccgagt acaacttggt ggcagaggag tacggaggcg aaacgatctt
      421 cgtcgaggtc tccgctaccc agggcaagaa catcgagaag cttctcgatg cggtgctgct
      481 gaccgcggat gccggccttg acctgcgtgc gaacccgaac aaggacgccc gtggcgtcgc
      541 ggtcgaggct cacctcgaca agggccgtgg ccccgtggcg accgtgctcg tccagtccgg
      601 aacgctgcgc gtcggcgatg cgatcgtggc cggcacggca tacggccgcg tgcgtgccat
      661 gttcaacgag cacgacgaac cggtcgcgga agcgctgccg gcacgtcccg tcatggtcat
      721 cggcctcaca tccgtgcctc gcgcaggcga cacgttcttg gccgccgagg acgaccgcac
      781 ggcacgtcag atcgctgaga agcgtgaagc cgccgagcgg gccgcccagc tggccaagcg
      841 tcgcaagcgc atctcgctcg aggacttcac gcaggcgatc gaggacggca aggtcaacac
      901 cctcaacctc atcatcaagg gcgatgtgtc cggtgccgtc gaggcactcg aggacgcttt
      961 gctcgcgatc gatgtgggcg attctgtgga tctgcgcgtg atccaccgtg gtgtgggtgc
     1021 cgtcacgcag aacgacgtca acctggccac cgtcgacagc gccgtcatca tcggcttcaa
     1081 cgtgcgtccc gccgagcggg tccaggagtt ggccgaacgt gaaggcgtcg acatgcgctt
     1141 ctactcggtc atctaccgtg ccctcgagga tgtcgaggcg tccctcaccg gcatgctcaa
     1201 gcccgaatac gaagagaagc agatcgggtc cgccgagatc atggagatct tccgttcctc
     1261 caagttcgga aacatcgccg gttgcctggt gcgcaatggc gtcatcaagc gcaacgccaa
     1321 ggcgcgcgtg gtgcgcagtg gcaacgtcat cggcgaaccg acgatcgaca cgctgcgtcg
     1381 cttcaaggac gatgccactg aggtcaagga cggcttcgag tgcggtattg gcctgggtaa
     1441 gttcaacgac ctccaggtgg gcgacatgat cgagacgttc gagatggtgg agattccgag
     1501 gtcctgatgg ccgactcacc ccgtgcactt cggctcgcgg gcacgatcaa gaagatcgtc
     1561 gcccgcgcgc tggagaatgt gatcaaggac ccccgtctgg ggttcgtcac cgtgaccgac
     1621 gtgcgcgtca cgggcgacct gcagcaggcg tcggtgttct acaccgtcta tggaggcgac
     1681 gaggatcggg ccaacacggc cgctgccctc gagtccgcca agggacgact gcgttcgatg
     1741 gtgggcggtg agctgggtat ccggctcacg ccttccctcg agttcttcct ggacgcggtg
     1801 ccggagacgg ccgcctcgct cgagaagtcg cttcacgagg ccgcactgcg cgacgccgag
     1861 ttggcgaagt tgcgggaggg cgccgctttc gccggagagg ccgacccgta ccgtcaccct
     1921 tccgacgagg acgactccga ggactgacgg cgtggcccgt cgcatcgacg agcccacccc
     1981 gggcctgctg cttgtcgaca agcccgcggg gtggacgtcg cacgatgtcg tcgcgcgttc
     2041 gcgccgcatc ctgaacaccc gcaaggtggg tcatgccggc acgctcgacc ccatggccac
     2101 cggcttgctg gtcctcgccg tgggacgtgc cacgcgactt ctcacgtacg tcacgggcca
     2161 cgacaagtcg tacgacgcca ccattcgcct tggccagtcc accatcacgg acgatgccga
     2221 gggcgaggtc acgtccacgg ccgacgcatc gtcgatatcg gacgatgccc ttgcggccgc
     2281 gatcgccgat ctgaccggcg acatctcgca ggttcccagc tcggtgagcg ccatcaaggt
     2341 caacggcgag cgcgcctacg cgcgcgttcg cgcgggcgaa gaggtcgcgt tggccgcgcg
     2401 ccccgtcacg gtgtctcgtt tcgaggtgac cgcggtgcgg cgcgaaggaa ccatgattga
     2461 cgtcgacgcc accatcgacg tgtcgtcggg gacctacatc cgcgccttgg cgcgcgacct
     2521 gggcgccgcg ttaggcgtgg gcggccacct gacggccttg cgccgcaccc gcatcggccg
     2581 ctttgacgtc gcgtcggcac gcaccgtcga tgcgctcgcg gcgctcgccg gctccggcgc
     2641 cgacatcccg ctcatcccac tcgacgaggc ggcgaaggcg gcgttgcccg tgttggcatt
     2701 gaccgagcag gaggccgtta gcctcggctt cggcaagccg ctgcgcgagc gggcggacga
     2761 actcaccaag cccgagcacg acgacgactt cacgcacgat gccgcagccg tcgcgccaga
     2821 cgggcgtgtg atcgcgctgg tgcgtcgggt gaagggcgcc gcgaagcctg tgatcgtctt
     2881 cctgccagcg ggggcggcgc gtgactgaga cgggtcggca ggtggggcag aatggccgga
     2941 tgacctgtg
//
LOCUS       DASRKH010000477         2474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_65963, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000477 DASRKH010000000
VERSION     DASRKH010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65963"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            354..>2474
                     /gene="ppdK"
                     /locus_tag="VFC06_07690"
     CDS             354..>2474
                     /gene="ppdK"
                     /locus_tag="VFC06_07690"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HZL81796.1"
                     /translation="MEKYVYEFSEGNRDLKDLLGGKGANLAEMTNIGLPVPPGFTITT
                     DACRIYMKTTKLPPELRVQVTMQLRRLEDSLGRLLGDTNDPLLVSVRSGAKFSMPGMM
                     ETVLNVGLNDASVEGLAAFAGDARFAWDSYRRLIQMFGKTVLDIDGELFSSALDAKKK
                     ARGVDADTDLPAEDLHALVDEFKAIVKAESGRDFPQQPREQLDLAIEAVFNSWNTDRA
                     RLYRRRERISDDLGTAVNVVAMVFGNLGDDSGTGVCFTRDPATGMVGDYGDYLPNAQG
                     EDVVAGIRNTLSLADFEKLDKKAFDELRQAMRTLESHYRDLCDIEFTVERGKLWMLQT
                     RVGKRTAAAAFRIANQLVDEHIITMDEALERVTGEQLNKLMFPQFDPNAKRKLLAKGM
                     AASPGAAVGAAVFSSATAQEWAERGKDVILVRRETNPDDLGGMIASVGVLTCRGGKTS
                     HAAVVARGMGTTCVVGAEALLVDANARTVTVGDRVVKEGDIIAIDGSTGEVFLGSVPV
                     IPSPVVRYLEEGLDVALSELAEDDENARDLVVAVHEILSHADAARRLGVYANADTPDD
                     AKRARTVGGEGIGLCRTEHMFLGERRELIEHVILATTDDERQAAFDALLPLQREDFIG
                     LLTEMDGLPVTVRLIDPPLHEFLPDLTELSVKVALAKERGETGAEVERDALLLPAVVR
                     MHEANPMLGLRGVRLGIVIPGLFTL"
BASE COUNT          468 a          798 c          803 g          405 t
ORIGIN      
        1 ccatctggca ctgcgtccag ggcaagaggc caccccggga cgtcacgacg tatggctcat
       61 ggactgtcat cgtcgctccc cgtgaacacg tggtgaacga tcaacggcgc cgccaggcta
      121 ctgaaacacg gcgcagcgaa gtgggaccaa ggtccacgag tggacggaca atatcgcaga
      181 cattcccagc cttcaatcgg tcacctcacc ctgtaatatc gggaaggcga ggttcgtcgc
      241 actctcaagg gagccttcca cccaggccca aagccccccc ttgggtgcac cgagcgtaga
      301 gagactgaga gggtcgcggc aatggcgccg ggacttgtct atggaggccc gccgtggaga
      361 agtacgtcta tgaattcagt gagggaaacc gagacctcaa ggatctcctt gggggcaagg
      421 gtgcgaacct tgccgagatg acgaacatcg ggctaccggt gcccccaggc ttcaccatta
      481 ctaccgacgc gtgtcgtatc tacatgaaga ccacgaagct ccctcctgaa ctacgtgtgc
      541 aggtcacgat gcagctccgg cgtctcgagg actcgctcgg acggctgctg ggcgacacca
      601 acgaccccct gctcgtctcc gtacgctcag gcgcgaagtt ctcgatgcca ggaatgatgg
      661 agaccgtcct caacgtggga ctcaacgacg cctcagtcga ggggctcgcg gccttcgccg
      721 gagacgcccg cttcgcctgg gactcatacc gccgcttgat ccagatgttc ggcaagacgg
      781 tgctcgacat tgacggcgaa ctgtttagca gcgcgctcga cgcgaagaag aaggcgcggg
      841 gggtcgacgc cgataccgac cttcccgccg aggacctgca cgccctcgtg gacgagttca
      901 aggccatcgt gaaggccgag tccggccgag acttcccgca acagccgcgc gagcaactcg
      961 acctcgcgat cgaagctgtg ttcaactcct ggaacaccga ccgtgcccgc ctgtaccgcc
     1021 ggcgtgagcg catttcggac gatctcggta ccgctgtcaa cgttgtggcg atggtcttcg
     1081 gaaacctcgg cgatgactcg ggaactgggg tgtgcttcac cagggacccc gccaccggca
     1141 tggtcggcga ctacggcgac tacctgccga atgcgcaagg cgaggacgtc gtggccggca
     1201 tcaggaacac gctgtccctg gccgacttcg agaagctcga caagaaagcc tttgacgaac
     1261 tgcgccaggc catgcgcacg ctcgagagcc actaccgcga cctatgcgac atcgagttca
     1321 ccgtcgagcg gggcaagctg tggatgctgc aaacccgagt gggcaagcgc accgcggctg
     1381 ccgcgttccg gattgcgaac caactggtcg atgagcacat catcaccatg gacgaggcgc
     1441 tcgagcgggt caccggcgaa cagctcaaca agctgatgtt cccccagttt gaccccaacg
     1501 ccaagcgcaa gttgttggct aagggcatgg ccgcctcccc cggcgccgct gtgggcgcgg
     1561 ccgtgttctc ctcggccacc gcacaagagt gggccgagcg gggcaaggac gtgatccttg
     1621 ttcgtcgcga gaccaacccc gacgacctgg gcggcatgat cgcctccgtt ggagtgctga
     1681 cgtgtcgggg cggcaagacg tcccacgcgg ccgtggtggc gcgcggcatg ggcaccacgt
     1741 gcgtcgtcgg cgccgaggcg ctcctcgtcg atgcgaatgc caggaccgtc accgtcggcg
     1801 accgagtggt caaagagggc gacatcatcg cgatcgacgg ctccacagga gaggtgttcc
     1861 tcggtagcgt gccggtgatc ccctcccccg tggtgcggta cctcgaagag ggtctcgacg
     1921 tcgcgctttc tgagctcgct gaggacgacg agaacgcgcg agacttggtc gtcgccgtcc
     1981 acgagatcct gagccacgca gatgcggcga ggcggttggg cgtctacgcg aatgcggaca
     2041 ctcccgacga cgccaagcgg gcgcggacag tgggcggcga aggcatcggc ctgtgccgca
     2101 cggaacacat gttcttggga gaacggcgcg aactgatcga gcacgtgatc ttggctacca
     2161 ccgacgacga gcggcaggcc gctttcgacg cgctgctccc cttgcagcgc gaggacttca
     2221 tcgggctgct caccgagatg gatgggctgc ccgtcacggt caggctcatc gacccaccgc
     2281 tccacgagtt cctgccggac ctgaccgaac tctccgtgaa ggttgccttg gcaaaggaac
     2341 gcggggagac gggtgccgag gtggagcggg acgcgctcct cctccctgcg gtggtgcgta
     2401 tgcacgaggc gaaccccatg ctcggtctgc gcggtgtgcg cctgggcatc gtgatcccgg
     2461 gcctgttcac gctg
//
LOCUS       DASRKH010000478         2785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_38894, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000478 DASRKH010000000
VERSION     DASRKH010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38894"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            263..1657
                     /locus_tag="VFC06_07695"
     CDS             263..1657
                     /locus_tag="VFC06_07695"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZL81797.1"
                     /translation="MSRAAWLTSERTRDYVTVWTIVLLMSGQGFRYMLGLPAYAVLSV
                     VTVAAVAVTMRTRVRDLRIPVLLAAFLGLAVASVVWSATRTVTALAIVVLLVTTYLAV
                     ATVRRTSPVRFMELLYRGLQVSLFGGVAFEVIVALVVRHPIAPLVGDLVKIADDDLGK
                     TNIMWSHDMLLSGGPIDGFVGNRNPFGAIALFAAIAGVALLMERRIRAMDGLATLATA
                     GVVHALTQSATVTIAAVYIGILALAALAIRRVPLRAKKAFSFTVLAVTAIAAVLTIKF
                     RAEIFAMVDRSSDATNRTEIWRQVISVAEQRPEGWGFVGYWPIWEQPYSGILDKVGLV
                     ATHSHNAFLDSWLQLGLIGFGLLLGLLVLTFGSAWRLVERAGRGDTFIPLGWAMLTAA
                     LALQALTESRLLVEGGWFLLVALYCSAPQVFTLTIVDPEYVHSATRRSEAEVGRAAAA
                     AASLPQEGEGDSPA"
     gene            complement(1691..2029)
                     /locus_tag="VFC06_07700"
     CDS             complement(1691..2029)
                     /locus_tag="VFC06_07700"
                     /inference="COORDINATES: protein motif:HMM:NF014814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZL81798.1"
                     /translation="MIKLDDWAEIRHLHSTGQRSKREIARLVGVSRGTVDRALAEDRA
                     PLAHRLPLRRQRRVKSRVRGSGIQSARRRGTFIPGHDADRQCLGHRHSWYELAPSPRP
                     QCASRPSHAG"
     gene            complement(2026..2301)
                     /locus_tag="VFC06_07705"
     CDS             complement(2026..2301)
                     /locus_tag="VFC06_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81799.1"
                     /translation="MNPQGPVSSAPPTRDGIHIRPWADRDLDVLRESNSPGMTAHIGG
                     PETDDAVVARHARYLRMWHTGEAHMFAIVDGAVLTPTENRATNHYGR"
     gene            complement(2313..>2785)
                     /locus_tag="VFC06_07710"
     CDS             complement(2313..>2785)
                     /locus_tag="VFC06_07710"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020013055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HZL81800.1"
                     /translation="YQASDPHLLRWVHIAEADSFLTAHQHFGARPLDPKRADEYVAQV
                     ARVAGALGATDVPTTTKELAECLESYRPELMATPAAIDTATFLLHEPPLPGAAKVPYS
                     LLAAGSVALLPQWAQTELHLGGGFNSRIGPWGGALATKAIRWGLSASLPDYPTL"
BASE COUNT          430 a          840 c          992 g          523 t
ORIGIN      
        1 tggggtcgac ggtgcttctg gaccccgatg cgagcccggc gttctgggcg gcgattgcga
       61 cgggtgatta cccaccgggg accgccgtgg gaggcatggg ctagcgccct ggcggaatcg
      121 gaagggggaa tccgtgcgtt aattggggga gagatcgcgt cgagtgagga agctccgcgg
      181 tcagggggca atcgtcaggt cgcttgcgac tatcggattc agggctgagg agctgtggcg
      241 aggaatggaa caggaaacac gcatgtcgag ggccgcctgg ctgacgtcgg agcgaacacg
      301 ggactacgtg accgtatgga cgatagtgct tctcatgtcc ggccagggct tccggtacat
      361 gctcggtctt cccgcttacg cggtcctgag tgtggtcacg gtcgcggccg ttgcggtgac
      421 gatgcgcaca cgggtccggg acctgcggat tccggtgctg cttgcggcgt tcttggggct
      481 cgccgttgcg agcgtcgttt ggtcggcgac gcgcacggtc accgctctcg cgatcgtggt
      541 gctgctcgtc accacctacc tcgccgtcgc gaccgtccgc cgcacgagtc cggtgcggtt
      601 catggagctg ctctatcgcg ggctgcaggt cagcctcttc gggggagtgg ccttcgaggt
      661 catcgtggcg ctcgtcgtga ggcatccgat agctccgctg gtcggtgacc ttgtcaagat
      721 tgccgacgac gacttgggca agaccaacat catgtggtca cacgacatgc tcctctcagg
      781 cggccccatc gacggattcg tcggcaaccg caatccgttc ggcgcaatcg cgctgtttgc
      841 cgcgatcgcc ggggtcgcgt tgctgatgga gaggcgcatt cgagcgatgg acgggctcgc
      901 gacgctggcg acggctggcg tggtgcacgc gctgacgcaa tcggccaccg tcaccatcgc
      961 agcggtgtac atcggcatcc tcgccctggc agcgctcgcc atccgccggg tcccgcttcg
     1021 cgccaagaag gcgttctcat tcacggttct cgcggtcacc gcgatcgcag cggttctcac
     1081 gatcaaattc agggctgaga ttttcgcgat ggtcgaccgg agttccgatg cgacaaaccg
     1141 caccgagatc tggcgccagg tgatctccgt ggccgagcaa cgaccggagg gttggggctt
     1201 cgtagggtat tggccgatat gggaacagcc ctactcggga atcctcgaca aggtgggctt
     1261 ggtcgccacc cactcgcaca atgccttcct ggactcgtgg cttcaactcg ggctgatcgg
     1321 gttcggactg ctgctgggtc tgctcgtact cacttttgga agcgcgtggc ggctcgtgga
     1381 gcgggccgga cgcggggaca ccttcatccc gttgggatgg gcgatgctca cggcggcgct
     1441 cgcgctgcag gcgctgaccg agtcgcgcct gttggtcgag ggtggctggt tcctgctggt
     1501 cgcgctgtat tgctccgcgc cacaagtgtt cacgttgacg attgtcgatc cggagtatgt
     1561 gcattcggcg acgcggaggt cggaagcgga agtaggtcgt gcggccgctg cggcggcctc
     1621 gctcccgcag gaaggcgagg gcgactcccc ggcgtgagtt accgcgtggg gtagttgggc
     1681 agcgagatgc tcaccccgcg tggctcggac gggaggcgca ttgcgggcgt ggcgatggcg
     1741 ctagttcgta ccaagaatgt cgatgaccca ggcattgacg gtcagcgtcg tgcccaggaa
     1801 tgaaagtacc tcgacgcctc gcagactgaa tcccgctgcc gcgcacacgc gatttgacgc
     1861 gtcgttgtcg acggagggga aggcggtgag cgagcggcgc ccggtcctcg gccaacgccc
     1921 gatcgaccgt cccgcgggac accccgacca gtcgggctat ctctcgcttc gagcgctgtc
     1981 ccgtcgaatg cagatggcga atctctgccc aatcatccaa tttgatcacc ttccatagtg
     2041 gttggtggcc ctgttttcag ttggcgttaa caccgcgccg tcaacgatgg cgaacatgtg
     2101 ggcctcgccg gtgtgccaca tgcgcaggta gcgcgcgtga cgtgcaacga cggcgtcgtc
     2161 cgtctcggga cctccgatgt gcgccgtcat gccgggcgag ttgctctcgc ggagcacatc
     2221 gaggtcacgg tccgcccacg ggcgaatgtg gatgccgtcg cgtgtcggcg gtgccgagga
     2281 aaccggaccc tgggggttca cagattcacg ccctacagcg tggggtaatc gggcagcgag
     2341 gcgctcagcc cccagcgaat ggccttggtc gcgagggcgc ccccccaggg gccgatgcgg
     2401 ctgttgaagc cgcccccgag gtgaagttcg gtttgcgccc actgcggcag cagcgccacg
     2461 gagccggccg ccaagagcga gtaggggacc ttcgccgcac ccggtagagg cggctcgtgg
     2521 agcaagaacg tcgccgtgtc gatggcggcg ggggtggcca tgagttccgg ccggtaggac
     2581 tcgaggcatt ccgcgagttc tttggtcgtg gtcggtacgt cggttgcacc aagcgcccca
     2641 gctacgcgag cgacctgcgc gacgtactcg tcggcgcgct tgggatccag ggggcgggcg
     2701 ccaaaatgct gatgagcggt gaggaacgag tcggcctcgg cgatgtgcac ccacctcagc
     2761 aggtggggat cgcttgcttg gtagg
//
LOCUS       DASRKH010000479         2065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_36827, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000479 DASRKH010000000
VERSION     DASRKH010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36827"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1916
                     /locus_tag="VFC06_07715"
     CDS             <1..1916
                     /locus_tag="VFC06_07715"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015771582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HZL81801.1"
                     /translation="TGDIPDGVLQRLRKVGPAQFAADLRAQTALAVTETTLRDAHQSL
                     LATRLRTFDMLQSAEHVARLTPQLLSVEAWGGATYDVAMRFLSEGPWKRLAALREAMP
                     NLPIQMLLRGRNTVGYTPYPDEVAHAFVHEAAATGVDIFRIFDALNDVDQMRPAIDAV
                     LETGTAVAEGTLCYTADLTNPGEKLYTLDYYLGVAEKLVKTGVHMLAIKDMAGLLRPP
                     AARTLVTALRKNFDVPVHLHTHDTAGGQLATLLAASEAGVDAVDVANAAMAGTTSQPP
                     MSALVAALEHTERDTGLSLDAVTDFEPYWEAVRRLYAPFESGLPGPTGRVYRHEIPGG
                     QLSNLRQQALSLGLGERFEDIENMYAAADRILGRLVKVTPSSKVVGDLALHLVGLGAD
                     PKDFAENPAAYDVPDSVIGFLRGELGDPPGGWPEPFREKALTGRGETKSMVSLSDDDR
                     AALDESGPARQRRLNHLLFPAPADDFDKAVELYGDISVIDTYHYLYGMTPGSGEEVNI
                     HMEKGVTMLVTLEAIGEPDDHGLRWVMFNYNGAIRPVQVLDQAAEVTVHAREKADVSV
                     PGQVAAPFAGAVTPLVAVGDTVEAGGAVATIEAMKMEASITTPVSGKVTRVVVNGTEP
                     LQGGDLVVVVDGA"
BASE COUNT          357 a          658 c          708 g          342 t
ORIGIN      
        1 ccacgggcga catccctgac ggcgtgctgc agcgcttgcg caaggtgggg ccggcccagt
       61 ttgccgccga cctgcgcgcc cagaccgcac tcgctgtcac ggagacaact ttgcgcgacg
      121 cccaccagag cctgctcgcg acgcggctgc gaaccttcga catgctgcag tcggcggagc
      181 acgtggcacg tctcactcct caactcctgt ccgtcgaggc atggggaggc gccacctacg
      241 acgtggcgat gcgcttcctc agcgagggcc cctggaagcg gctggcggcc ctgcgggagg
      301 cgatgcccaa tctgccgatc cagatgctgc tgcgaggccg caacacggtc ggctacacgc
      361 cgtacccgga tgaggtcgcg cacgccttcg tgcacgaggc cgcggccacg ggcgtcgaca
      421 tcttccgcat cttcgatgct ctgaacgacg tcgaccagat gcgacctgca attgacgctg
      481 tgctggagac gggcaccgcg gtggcggagg ggacgttgtg ctacacggcg gacctcacga
      541 acccgggcga gaagctctac acgctcgact actacctggg cgttgcggag aagctcgtga
      601 agacgggcgt ccatatgctg gccatcaagg acatggcggg gctgttgcga cccccggccg
      661 cacgcactct tgtgacggcc ctcaggaaga actttgacgt ccccgtgcac ctgcacaccc
      721 acgacacggc tggcggtcag ctcgctacct tgctggccgc gtctgaggcc ggggtggacg
      781 cggtggacgt cgccaatgcg gccatggctg gcacgacgtc gcagccgccg atgtcggctc
      841 ttgtggccgc tctcgagcac accgaacgcg atacgggcct gtctctagac gcggtgaccg
      901 acttcgaacc ctattgggag gccgtgcgcc gcctgtacgc gcctttcgag tccggtctgc
      961 ccggacccac ggggcgtgtg taccgtcacg agattcccgg cgggcagctg tccaacctgc
     1021 gccagcaggc gctgtcgctg gggctggggg agcggttcga ggacattgag aacatgtatg
     1081 cggccgccga ccggatcctg ggtcgactgg tcaaggtcac gccctcctcc aaggtcgtcg
     1141 gcgacctcgc gctgcatctc gtcggcctgg gcgccgatcc caaggacttt gccgagaatc
     1201 ccgcggccta cgacgtccca gactccgtga tcggcttcct gcgcggcgaa ctgggcgacc
     1261 cgcccggagg ctggcccgag ccgttccgtg agaaggcgct caccggtagg ggagagacca
     1321 agtccatggt gtctctcagc gacgatgacc gagcggcgct ggacgaatcg ggcccggcgc
     1381 gccaacgtcg gctcaaccat ttgctgttcc cggcgccggc cgacgacttc gacaaggccg
     1441 tcgagttgta cggcgacatt tcggtgatcg acacctatca ctacctgtac ggaatgacgc
     1501 cgggcagtgg ggaagaggtc aacatccaca tggagaaggg cgtcacgatg ttagtgacac
     1561 tggaggccat tggtgagccc gacgatcacg ggctccgctg ggtgatgttc aactacaacg
     1621 gtgcgatcag gccggtgcag gttctcgatc aggcggctga ggtcacggtg cacgctcgtg
     1681 agaaggcgga cgtgtcggtg cctggccagg tcgccgcgcc ctttgcgggc gccgtgacgc
     1741 cgctcgtggc cgtcggggac acggtggaag ccggcggagc tgtcgctacc atcgaggcca
     1801 tgaagatgga agcgtcaatc accacgccgg tgagcggaaa ggtgacacgg gtggtcgtga
     1861 acggcaccga accgctccag ggcggcgatc tcgtcgtcgt ggttgacggt gcatagcggc
     1921 agggactctt cgagtcgtcg agcctggtac atcagatcca tctgggacca cgggaccttt
     1981 gacctgaacc cgcgcagggc aggcgagaga acatggcagg caggaggtgc atcatgaagg
     2041 ttctggtagc agtggggtca aagta
//
LOCUS       DASRKH010000480         2949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_16669, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000480 DASRKH010000000
VERSION     DASRKH010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16669"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..1596
                     /locus_tag="VFC06_07720"
     CDS             133..1596
                     /locus_tag="VFC06_07720"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015772785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HZL81802.1"
                     /translation="MTNQPAPAVPPRDVLAARAGALGAAVPSEVAELAALFDAAGHEF
                     ALVGGPVRDAFLGRSSADFDFATSARPDETERLLKKWTHATWDMGRDFGTIGGRRGDL
                     VVEVTTFRADEYDGATRKPVVAFGDSLEGDLARRDFTVNSMAVRLPGGEFVDPFGGLD
                     DLAGGLLRTPIEPGLSFGDDPLRMMRAARFASQLGFEIEPATFAAMHAMADRIEIVSA
                     ERVRDELTKLMMGACVRDGLSALVDSGLADQVLPELPALKLEVDEHHRHKDVYEHTLT
                     VVDQAIDLETDAEGPVPGPDLVLRLAALLHDIGKPATRRFEPGGTVSFHHHELVGAKM
                     AAKRLKALRFDKDTIKAVAHLVELHLRFHGYGEARWSDSAVRRYVTDAGSQLERLHRL
                     TRADVTTANRRKAERLAFAYDDLENRITELRAKEEIDSIRADLDGTQIMAELGIGPGR
                     DVGLAYKHLLELRMDHGPLGDERARAELHAWWESRQA"
     gene            2084..2683
                     /locus_tag="VFC06_07725"
     CDS             2084..2683
                     /locus_tag="VFC06_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81803.1"
                     /translation="MTRLTTTVPVAWWCGDIRLATPEYALALGYGDESAGRRAELVYR
                     SVRQGVVRVPAALAALDRLPRVRGRASLVDRLELAARGVESFLEERGLRDLFLGEVLG
                     RVLWQHTVRVGADRYRIDAYDPVSGTAFELDGSQWHDGSKERLRDMRRDAALATIGIV
                     TIRFGYRDVVERPRWCAEVARAAIAARGSNAWSTARRTG"
     gene            complement(2739..>2949)
                     /locus_tag="VFC06_07730"
     CDS             complement(2739..>2949)
                     /locus_tag="VFC06_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HZL81804.1"
                     /translation="DRANMPGAVGNTSTIALTNATLPFALAIAGKGWDVASAGDPALA
                     KGLAVSGGLIRNAPTAAAFPHLPAT"
BASE COUNT          461 a          968 c         1045 g          475 t
ORIGIN      
        1 ggcccaactc taaccgggag cgaggccagc gaccggcagc gccccccgcg ttgacgctcg
       61 ccgcgggcac cgcccatggg tgtccggccc gcgaagacat ggttccggcg cggctctggg
      121 atgctggtac ccatgaccaa ccagcctgcc cctgccgtgc caccccgtga cgtgctcgcg
      181 gcgcgcgcgg gagccttggg agcggcggtg ccttcggagg tcgcagaatt ggcggcgctc
      241 ttcgatgcgg ctggtcacga gtttgcgctc gttgggggtc ccgttcgcga cgccttcctg
      301 ggccggtcaa gcgccgactt cgactttgcc acgtccgcga ggccggacga gacggagcgg
      361 ctcctcaaga agtggacgca cgccacgtgg gacatggggc gcgacttcgg caccatcggc
      421 gggcggcgtg gagacctcgt ggtggaagtc accaccttcc gcgctgacga gtacgacggc
      481 gccacccgca aacctgttgt cgcttttggc gactcgctgg agggcgacct cgccaggcgc
      541 gacttcaccg tcaactcgat ggctgtgcgg ttgcctggcg gcgagttcgt ggatcccttt
      601 ggcgggcttg acgaccttgc cggtggcctg ctgcgcaccc ccatcgaacc cggattgtcc
      661 tttggcgacg accccctgcg catgatgcgg gccgcccgtt tcgcctctca actgggcttc
      721 gagattgagc cggccacgtt tgcggcgatg cacgcgatgg ccgatcggat cgagatcgtc
      781 tcggcggagc gagtgcgcga cgaactcacc aaactgatga tgggcgcgtg cgtccgcgac
      841 ggcttgagcg cgcttgtcga ctcgggcctt gccgaccagg tgctgcccga gctgcccgcc
      901 ctcaagctcg aggtcgacga gcaccatcgg cacaaggacg tctacgaaca caccctcacg
      961 gtggtggacc aggcgatcga tctcgagacg gacgcggagg gtcccgtccc aggacccgac
     1021 ctggtgctgc gattggcggc actgctgcac gacatcggca agccggccac acgcaggttc
     1081 gagcctggcg gcacggtgtc gttccaccac cacgagctgg tgggcgccaa gatggcggcc
     1141 aagcggctca aggcgctgcg cttcgacaag gacaccatca aggcggtggc tcacttggtc
     1201 gaactgcacc tgcgcttcca cgggtatggc gaagcgcgct ggtccgactc tgcggtccga
     1261 cgctacgtga cggacgccgg ctcgcagttg gagcgactgc atcggctcac gcgcgccgac
     1321 gtgacgacgg ccaaccggcg caaggccgaa cgactcgcgt tcgcgtacga cgatctcgag
     1381 aaccgcatca cggagttgcg cgccaaggag gagatcgact ccatccgcgc tgacctggac
     1441 ggcacgcaga tcatggccga gttggggatc ggtcccggtc gcgacgtggg cctggcctac
     1501 aagcatctgc tggagttgcg catggaccac ggcccgctcg gcgacgagcg tgcgcgggca
     1561 gaactgcacg cgtggtggga aagccgccag gcctagacgg caacgcggta ccgcgccgtc
     1621 acccggcccc aacgccctgc ggtacatgac gcgctcacag cgcgcgttgc ccacagccag
     1681 gcggtacgta gcgcattccc cgacatgcgt acgcgaccca gccgcacgtc ggccgagccc
     1741 cggcagcatc gccgtcatga ccctcaagga agtgctcgcc gtggcacacg gcactcctcg
     1801 accattcacg gcggaagagc tctcgggtat ggcgagtcgc accggtctgg cgaacgcggt
     1861 ctgccgcggg gaactagtcc gcgtactgcc gcgcctctat gcctcgtcgg tccacgccga
     1921 ctcgttcatc gtccgcgccc gggcggcgtg caggtgggcg ccggaagccg cggtgtcggg
     1981 ggcatcggct ctgtttgcat acggggcgtg cgacgagccg ccaccccgtc tgaggatctc
     2041 gatgcctcgt cacgcgcatc gacgattccc cgagtgagtg cacgtgacgc ggttgaccac
     2101 cacggtgcct gtcgcatggt ggtgtgggga tatacgactg gccacgcccg agtacgcgct
     2161 cgcgttgggg tacggcgatg agagcgcagg ccgccgcgcc gagttggtct atcggtcggt
     2221 gcggcaaggt gtggtccggg tgcctgccgc gctcgcggcg ctagatcgcc tgccgcgggt
     2281 gcgcggtcgc gcttcgctcg tcgacaggct cgaacttgcc gcgcgcggag tggagagctt
     2341 tcttgaggag cggggcctgc gcgacctgtt cttgggagag gtgttggggc gcgtgctgtg
     2401 gcaacacacg gtgcgcgtgg gtgccgaccg gtaccggatc gacgcttacg atcccgtgtc
     2461 cggaaccgcc tttgagctcg acggttcgca atggcacgac ggatcgaagg aacggctacg
     2521 cgacatgagg cgcgacgcgg cattggcgac gatcggaatc gtcacgattc ggttcggata
     2581 tcgagacgtt gtcgagcgac cccggtggtg tgcggaagtg gcacgagccg ctatcgcggc
     2641 gcgaggttcg aacgcatgga gtaccgccag gaggacgggg tgagcgcgaa cgaatgcgtg
     2701 gtgtaccgca ggcaggacgg ggtgagtgcc accgggcatt acgtcgcggg gaggtggggg
     2761 aaggccgccg ccgtgggcgc gttgcggatc aatccacccg agaccgctag ccccttggcg
     2821 agggcaggat caccggccga tgccacgtcc cagcccttgc cagcgatcgc caacgcgaag
     2881 ggcagggtcg cattcgtcag cgcgatcgtg gacgtgttcc ctacggcgcc gggcatgttg
     2941 gccctgtct
//
LOCUS       DASRKH010000481         2070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_16683, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000481 DASRKH010000000
VERSION     DASRKH010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16683"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1603)
                     /gene="pknB"
                     /locus_tag="VFC06_07735"
     CDS             complement(<1..1603)
                     /gene="pknB"
                     /locus_tag="VFC06_07735"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HZL81805.1"
                     /translation="MVDNIPRVLAGRYEVGELIGRGGMAEVHIGYDSRLSRTIAIKLL
                     RGDLAEDPTFHARFRREAQSAAALNHPAIVAVYDTGEETTTDALGKQTTLPFIVMEYV
                     EGHTVRELLKDGAAVPIDEAVEILVGVLGALEYSHNEGIVHRDIKPGNIMLTPTGQVK
                     VMDFGIARAMADTSATMTQTNSVVGTAQYLSPEQARGEVVDARSDLYSTGCVLFELLT
                     GQPPFSGDSAVSVAYQHVRELPPVPSTIASDIPESLDRVTMKALAKDRDERYADANQM
                     RGDLLAVLHGGTIDAPVATAWLPVGGVGAPAATTQLTAAAPADATRVSHATTTIPPAR
                     PLSDDSEKGSRWWVWLLALVGLAAAIGIAWMVMNRDNTGAEMVPVPDVTSLDQAAAAA
                     AIEDGDLLKFNYAGEEANADIAAGLAVRSDPAALQEVEVGTTVDVYFSSGPEEFTIPP
                     VENMTQAQAQKVLTDAGLFVSSVETEDAPRIQRNVALRTDPAAGEPAKKGDSVVLYIA
                     TGRVQVLSVIGQSEAEATNYLQNELG"
     gene            complement(1597..>2070)
                     /locus_tag="VFC06_07740"
     CDS             complement(1597..>2070)
                     /locus_tag="VFC06_07740"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZL81806.1"
                     /translation="VLTDFGVSHAVGQGRITAVGHVMGTAEYMAPELIAGVPASPASD
                     VYALGICAFEALTGAVPFRGPNPVQTALAHVDLPAPGLPATVPAFLRDRVAAMLAKDP
                     VLRPTTLRVGAWARELVDSANTLTAPEGADTLEGDVHGASPADHTNPQHTEEDKW"
BASE COUNT          271 a          700 c          767 g          332 t
ORIGIN      
        1 ggcccaactc gttctggagg tagttggttg cctcggcctc ggactggccg atgacgctca
       61 gcacctgcac cctccccgtg gcgatgtaca gcaccacgct gtcgcccttc ttggcgggct
      121 cgcccgcggc ggggtcggtg cgcagcgcaa cgttgcgttg gatacggggc gcgtcctcgg
      181 tctccacgct gcttacgaac agcccggcgt cggtcagcac cttctgggcc tgcgcctggg
      241 tcatgttctc cacgggcggg atggtgaact cctccggtcc ggaggagaag tacacgtcga
      301 cggtggtgcc gacctcgacc tcctggaggg cggccgggtc cgaccgaacg gcgaggcctg
      361 ccgcgatgtc cgcgttggcc tcctcgccgg cgtagttgaa cttgaggagg tccccgtcct
      421 cgatcgccgc ggcggcggcg gcctggtcca ggctggtcac gtcgggcacg ggcaccatct
      481 cggcgccggt gttgtcccgg ttcatcacca tccaggcgat cccgatcgcg gcggcgaggc
      541 cgacgagggc cagcagccag acccaccagc gcgaaccctt ctccgaatcg tcggagagtg
      601 ggcgggccgg cgggatggtc gtcgtcgcgt ggctcacccg cgtggcgtcg gccggcgcgg
      661 cggcggtgag ctgtgtcgtc gcagccgggg ccccgacgcc gccgaccggc agccacgccg
      721 tggccacggg cgcgtcgatg gtgcccccgt gcagcacggc cagcaggtcg ccgcgcatct
      781 ggttggcgtc cgcgtagcgt tcgtcgcggt ccttggcgag ggccttcatg gtgacgcggt
      841 cgagggactc cgggatgtcg ctcgcgatgg tggacggtac gggagggagc tcgcggacgt
      901 gctggtaggc cacggacacc gcggagtcgc cggagaaggg cggctggccg gtgaggagtt
      961 cgaagagcac gcagccggtg gagtacaggt ccgagcgggc atccacgacc tcgccgcggg
     1021 cctgttcggg ggagaggtat tgcgcggtcc ccaccacgga gttggtctgg gtcatggtgg
     1081 cggacgtgtc cgccatggcg cgcgcgatgc cgaagtccat gaccttcacc tggcccgtgg
     1141 gggtcagcat gatgttcccg ggcttgatgt cgcggtggac gatgccctcg ttgtgggagt
     1201 actcgagcgc cccgaggacc ccgaccagga tctccacggc ctcgtcgatc gggacggcgg
     1261 cgccgtcctt caggagttcg cggaccgtgt ggccctccac gtactccatg acgatgaagg
     1321 gcagggtggt ctgcttgccc agcgcgtcgg tggtggtctc ctcgccggtg tcgtacaccg
     1381 ccacgatcgc ggggtggttg agcgcggccg cggactgcgc ctcgcggcgg aagcgcgcgt
     1441 ggaaggtggg gtcctcggcg aggtcacccc ggaggagctt gatggcgatc gtccgcgaga
     1501 ggcgggagtc gtacccgatg tggacctcgg ccatgccgcc gcgcccgatc agctcgccca
     1561 cttcgtaccg gcccgccagc acacgcggga tgttgtctac cacttgtcct cctcggtgtg
     1621 ctgagggttg gtgtggtccg ccggcgaagc gccgtggaca tccccctcca gcgtatcggc
     1681 cccttctggg gcagtgagag tgttcgcgct gtccaccaac tcacgcgccc aagcacccac
     1741 gcgcagcgtg gtgggccgga gcaccgggtc cttcgcgagc atcgcggcga cccggtcccg
     1801 caggaatgcg ggcacggttg ccggcagtcc gggcgcgggc aggtccacgt gcgcgagggc
     1861 cgtctgtacc gggttcgggc cccggaaggg caccgcaccg gtcagggcct cgaaggcgca
     1921 gatcccgagc gcgtacacat ccgaagccgg cgacgccggt acgcccgcga tcagttccgg
     1981 cgccatgtac tcggcggtgc ccatcacgtg cccgacggcc gtgatccggc cctgccccac
     2041 cgcgtgggac accccgaagt cggtgagcac
//
LOCUS       DASRKH010000482         2291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_57355, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000482 DASRKH010000000
VERSION     DASRKH010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57355"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /locus_tag="VFC06_07745"
     CDS             complement(<1..973)
                     /locus_tag="VFC06_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HZL81807.1"
                     /translation="MSPVPFAFPAPLALPTAPADPGSLESIATPTLWAWTIGGILALF
                     VLDFLITRKPHEVSMREATGWSIFYVGLPLSFGGWLWWQYGQQQGLEYLTGYLVEKSL
                     SVDNLFVFMLILSGFAVPKVLQQRALLIGVAGALVMRGIFIALGATLIANFAVTFLFF
                     GLILLATAVHVWRDAASEEDTHKDVSDLRIVRWIRRFFPVTDDYRGTHMTVRDAGRRA
                     LTPLAVVTLAILGTDLMFAIDSVPAVYGITGDPYLVFVTNAFALLGLRALYFVLEGVL
                     SRLVHLGYGLAIILAFIGVKLVLHWAHGIWAGVPTVPTLLSLAVIVGI"
     gene            complement(1118..1642)
                     /locus_tag="VFC06_07750"
     CDS             complement(1118..1642)
                     /locus_tag="VFC06_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81808.1"
                     /translation="MSASGRFRSRRVPYGALVGLAFVCLAVVLLVLAGGPTTEQPDGQ
                     VRVTFLWESDCGQEGAPFELEIEDAQHEILGVWSLNRQSSHPLAIDGRRLCGYTLAFP
                     DLPPSDEYTFLVYDLYGPEREQDYRESAAAQHRAAQLTRTRTELDAAGWSVVVFYDPT
                     GTTQRFFFTEVAAR"
     gene            1753..>2291
                     /locus_tag="VFC06_07755"
     CDS             1753..>2291
                     /locus_tag="VFC06_07755"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HZL81809.1"
                     /translation="MKATRFTDSVAHQGEGPVWWATTGELRFVDMLHGAVVAFTAADE
                     TYHLPVDSPVAAVLRPRVGGGAIVARELDIALARAEDLSDLAPCTPPFVPAGQRLNEG
                     GCDPDDTVTTPAVATPTVVTPDVTISNGLGWSPDGARAYYIDTPTRTVSLLDYSRDAG
                     LTDRRPWVVPPEEAGRPDGL"
BASE COUNT          379 a          789 c          804 g          319 t
ORIGIN      
        1 ggatcccgac gatcaccgcg agcgacagga gcgtgggcac ggtgggcacg ccagcccaga
       61 tcccgtgcgc ccagtgcagc accagtttga cgccgatgaa ggcgaggatg atcgcgagcc
      121 cgtagccgag gtggacgagg cgggacagca cgccttcgag gacgaagtaa agcgcgcgca
      181 gcccgagcag cgcgaacgcg ttggtgacga acacgaggta ggggtctccg gtgatgccgt
      241 agacggcggg gactgagtcg atcgcgaaca tgaggtccgt gcccaggatg gcgagcgtga
      301 ccacggcgag cggggtgagg gcgcggcgtc cggcgtcgcg cacggtcatg tgggtgccgc
      361 ggtagtcgtc ggtgacgggg aagaagcgcc ggatccagcg gacgatgcgg aggtcggaga
      421 cgtccttgtg ggtgtcctcc tcggaggcgg cgtcccgcca gacgtgcacc gccgtggcga
      481 gcaggatgag cccgaagaag aggaacgtga ccgcgaagtt ggcgatcagg gtggccccga
      541 gcgcgatgaa gatcccgcgc atgacgaggg cgccggctac gccgatcagc agcgcccgct
      601 gttgcagcac cttcggcacc gcgaagcccg agaggatcag catgaagacg aacaggttgt
      661 ccacgctgag ggacttctcc accaggtagc ccgtgaggta ttcgaggccc tgctgctggc
      721 cgtactgcca ccagagccac ccgccgaagc tgagcgggag cccgacgtag aagatcgacc
      781 acccggtggc ctcgcgcatc gagacctcgt ggggtttccg ggtgatcaga aagtccagga
      841 cgaacagcgc caggatgccg ccgatcgtcc acgcccacag cgtgggggtg gcgatgctct
      901 cgagggagcc gggatcggcc ggggcggtcg ggagggcgag cggtgcgggg aaggcgaacg
      961 gtacgggtga catgtgggtc tcctcagatc gtcgaacggt ctgaggtctc cttcaccgcg
     1021 gtgcggcgcc cgtgccgggg tcgccatggg gcgtccgtac tgtccggcac gaggcttgcg
     1081 aagtactccc ctcgtggctt ccatggtaat tgacagttca acgagccgca acctcggtga
     1141 agaagaaccg ctgcgttgtg cctgtggggt cgtagaacac gacgaccgac cagcccgcgg
     1201 cgtcgagttc ggtgcgggtc cgggtgagct gggcggcgcg gtgctgggcc gcggcactct
     1261 cccggtagtc ctgctcgcgc tcgggaccgt agaggtcata cacgaggaag gtgtactcgt
     1321 cggacggcgg caggtccggg aacgcgaggg tgtacccgca caggcgccgg ccgtcgatgg
     1381 ccagggggtg gctcgactga cgattcaggc tccacacacc gaggatctcg tgctgggcgt
     1441 cctcgatctc cagttcgaac ggggctcctt cctgcccaca gtccgactcc cacaggaagg
     1501 tgacccggac ctgcccatcc ggctgctcgg tcgtcggccc cccggccaag accagcaaca
     1561 ccaccgccag gcacacgaac gccagaccca cgagcgcacc gtacggcact cgtcggctgc
     1621 ggaatcttcc tgacgcgctc atctccggca cccttcggcg atccgcggga cgcctcaagg
     1681 ctcccacgcc aagtgtcggc gcccacccgg ttccgtcgcg ccccatcccg cccccggcct
     1741 accatcgaga ccatgaaggc cacccgtttc actgactccg tcgcccacca aggtgaaggc
     1801 cccgtgtggt gggccaccac cggcgagctg cgcttcgtgg acatgctgca cggcgccgtc
     1861 gtcgcgttca cggcggccga cgagacgtac cacctcccgg tggactcccc cgtcgcggcg
     1921 gtcctccgcc cccgcgtggg tggcggcgcg atagtggccc gcgagctgga catcgccctg
     1981 gctcgcgcgg aggacctctc cgacctcgcc ccctgcacgc cgccgttcgt ccctgccggc
     2041 cagcgcctca acgagggcgg ctgcgacccg gacgacaccg tcacgacgcc cgccgtcgcg
     2101 acccctaccg tcgtcacccc cgacgtgacc atctccaacg gcctcgggtg gagcccggac
     2161 ggcgcccgcg cgtactacat cgacaccccg acgcgcacgg tctcgctcct cgactacagc
     2221 cgggatgccg ggctcaccga tcgccggccg tgggtggtgc cgcccgagga ggcgggccgc
     2281 ccagacggcc t
//
LOCUS       DASRKH010000483         2665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_3326, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000483 DASRKH010000000
VERSION     DASRKH010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3326"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /locus_tag="VFC06_07760"
     CDS             complement(<1..1128)
                     /locus_tag="VFC06_07760"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZL81810.1"
                     /translation="MDAEAARLATSPETLSLLASLPPYDGDHALTLGQQLRDKGANAG
                     VVSAALTQSRLRAAARPKFGDFASGMLFSPEGLEQSTRLVVAAHHAQRYRAAGVRTVA
                     DLTCGIGADAMAFASLGLGVLAFELDEGTALLADHNLRHWDDARVVHADAMETLARGD
                     IGVDALFADPARRNARGRRHDPRDYSPPLPDVVALRGRWPHVGIKVGPGIAHEDVPPD
                     AEAQWVSVDGDVVEAGLWMGDLARHHEHSALVLRGDDAHVLDGSPVPGEPGSIGEYLL
                     EPDGAVIRAGLVGPLSERLGARLLDPHIAYLTADARIESPFAATFRIVESLPYSEKRL
                     ASALAARGVGTAEIKKRGLNVDPAVLRRKLKLKGDASATVIL"
     gene            1313..1606
                     /gene="groES"
                     /locus_tag="VFC06_07765"
     CDS             1313..1606
                     /gene="groES"
                     /locus_tag="VFC06_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HZL81811.1"
                     /translation="MSVSIKPLEDKVVVKPAVAEQTMASGLVIPDTAKEKPQEGEIVA
                     VGPGRIDDNGNRVPLDVAVGDRVMYSKYGGTEVKHGGQEYLILSVRDLLAVVE"
     gene            complement(1735..2025)
                     /locus_tag="VFC06_07770"
     CDS             complement(1735..2025)
                     /locus_tag="VFC06_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HZL81812.1"
                     /translation="METIATLPAPTQDQWEWQYEGSCREADPNLFFHPEGERGAARRR
                     RAEAAKQFCNRCPVLDLCRERSLAAREPFSVWGGLSEDERHAILAGRLKQAS"
     gene            2313..>2665
                     /locus_tag="VFC06_07775"
     CDS             2313..>2665
                     /locus_tag="VFC06_07775"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HZL81813.1"
                     /translation="MAPSEHDPFGFIGLTYDDVLLLPGESDVTPSEVSTKTRLTRHID
                     IAVPLLSAAMDTVTEARLAIALARLGGIGVLHRNLSIEDQAQQVVTVKRSESGMITDP
                     VTVGPDASLAELDAL"
BASE COUNT          442 a          879 c          889 g          455 t
ORIGIN      
        1 gaggatgacc gtcgcgctcg cgtcgccctt gagcttaagt ttgcgccgta ggacggcggg
       61 gtccacattg agtccgcgct tcttgatctc cgcagtgccg acgccgcgcg ccgcgagtgc
      121 ggaggccaag cgcttctccg aatacggcaa cgactccacg atgcggaagg tcgcggcaaa
      181 gggagactcg atgcgggcgt cggccgtgag gtaggcgatg tgcgggtcga gcaggcgggc
      241 gccgagccgc tcggacagcg gccccacgag tcccgcccga atcacggcgc cgtccggctc
      301 cagcaggtac tcgccgatcg agccaggctc gcccggcacg ggggagccgt ccagcacgtg
      361 tgcgtcatcg ccgcgcaaga cgagggccga atgctcgtga tgacgcgcca ggtcccccat
      421 ccacagcccg gcctcgacca catcgccgtc cacgcttacc cactgggcct ccgcgtccgg
      481 cggaacgtcc tcgtgagcga tgccgggtcc taccttgatg cccacgtgtg gccaccgccc
      541 acgcagagcg accacgtcgg gtagcggagg cgaatagtcg cgtgggtcgt ggcgccggcc
      601 tcgggcattg cgccgtgcgg ggtccgcgaa cagggcatcg acgccgatgt caccccgggc
      661 gagcgtttcc atggcgtcgg catggacgac gcgggcatcg tcccagtggc gcaagttgtg
      721 atctgccagc agcgctgtgc cctcgtccag ctcgaatgcg aggacgccaa ggccgagcga
      781 cgcgaacgcc atcgcgtcgg cgccaatccc gcacgtcagg tcggctaccg tccgcactcc
      841 cgcggcgcgg taccgctgcg cgtggtgggc ggcgaccacg agccttgtgg actgctcaag
      901 gccctcggga ctgaagagca tccccgaggc gaagtctccg aacttcgggc gggcggcggc
      961 acgcagccgc gactgcgtga gagcggcgga gacgacaccc gcattggcgc ccttgtctcg
     1021 taattgctgg ccgagcgtca aggcgtggtc gccgtcatag ggaggcaggc ttgccagtag
     1081 cgagagcgtc tctggggagg ttgccaggcg tgcggcttct gcgtccatcc ctcgatcctg
     1141 gcatgcccgc gatttctcct cgggcgaaag ggtgtcggcg ggttagcact caggccccga
     1201 gagtgctaaa ttctgaagtg ctcccagttg ggagagcggc cattgcccga cccccgcgac
     1261 ggcgggcttc ggtcgcaatc aaacgtcacg ttctaggaag gtgaggtccg cagtgtcggt
     1321 ctcaatcaag cctctcgagg acaaggtcgt ggtgaagccg gcagttgccg agcagaccat
     1381 ggcctcgggc ctcgtgatcc ccgataccgc gaaggagaag ccccaagagg gcgagatcgt
     1441 cgcggtagga ccgggtcgta tcgacgacaa cggtaaccgc gttccccttg atgtcgccgt
     1501 tggcgatcgg gtcatgtact cgaagtacgg cggcaccgag gtcaagcacg gcggtcagga
     1561 gtacctgatc ctgtccgtgc gcgacctgct cgcagtcgtc gagtaagtct ctttgcacga
     1621 actaaggccc cggagacctg atggtcttcg gggccttagt gttctccgtg cggccccaac
     1681 ggcgtgagga gggttaggcg gcgggcgcag tggagatggc cgagaagcgg gtggtcaact
     1741 tgcctgcttc aggcggcccg cgaggatcgc atggcgttcg tcctcgctga gtcctcccca
     1801 cacgctgaag ggttcgcgtg ctgcaagcga gcgttcccta cacaagtcaa ggacggggca
     1861 gcggttgcag aactgcttgg cagcctccgc gcgccgtcga cgtgccgcac cgcgctcacc
     1921 ctcagggtga aagaagaggt tcgggtcggc ttctcgacaa gagccctcgt actgccattc
     1981 ccactggtct tgcgtggggg cgggcagtgt ggcgatcgtc tccatcgtgc tcctcgctcg
     2041 gtggttcaca cttttgtctt cattgaccgg ggctcgtccc gggagtcact tcctgccacg
     2101 gaatcaccat aaaccgggct aaacggacag tcaagagagt ccggttcact gctaactcca
     2161 cctctgtttc tactcccttg gcgaggggtt gggaaccccc catttcgggg gccatgccgc
     2221 ctctccggca ggcgggcgtt tggaaaatgc caccctcggt atgtccgctc cggcgactcg
     2281 acgaccgggg atggagcctt agacttgggc ccatggcacc cagcgagcac gaccccttcg
     2341 ggttcatcgg cctcacgtac gacgacgtcc tcttgcttcc gggcgaaagc gacgtcacac
     2401 cctccgaggt gtcgaccaag acgcgactca ctcgccacat cgacatcgcg gttcccctgc
     2461 tgagcgcggc catggacacc gtgaccgagg cgcgcctcgc gatcgcgctg gcgaggctcg
     2521 gaggcatcgg tgtgctgcat cgcaacctct ccatcgaaga ccaggcgcag caggtcgtca
     2581 ccgtcaagcg ctccgagtcc ggcatgatca ccgaccccgt cacggtcggg cccgacgcta
     2641 gccttgccga actggacgca ctgtg
//
LOCUS       DASRKH010000484         2099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_3373, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000484 DASRKH010000000
VERSION     DASRKH010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3373"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1257
                     /locus_tag="VFC06_07780"
     CDS             <1..1257
                     /locus_tag="VFC06_07780"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HZL81814.1"
                     /translation="GVGYDVVVFTGVGAGGATVERLRQAAGAATRCLAGVSRLDSALP
                     ADFPELVGSLTEGFALGAYRFDRYRTAESAVGTVRILTPLATDPLAAAAVERGLILAY
                     AVNTARELVNTSPADLYPETLADAAVRLAAEHGLEATVLAVPELEKGGFGGILAVGQG
                     SVRPPRLVKLVHRPAGATAHMALVGKGITFDSGGLSLKPAKGMGDMKSDMAGAAAALG
                     AVVAAARLGLPVRVTVWLALAENMPSGSATRPSDVITMRGGRTVEVLNTDAEGRLVLA
                     DALLAATEEDPDVVVDIATLTGAQMVALGNRVSAVMGDDDTRSDVLAAADAAGEQFWP
                     MPLPAELAESLKTPMADIANIGERNGGMLVAGLFLQSFVEGRRWAHLDIAGPALNTQK
                     AWGYTPMGGTGVGVRTLVNLLEAATP"
     gene            1351..>2099
                     /locus_tag="VFC06_07785"
     CDS             1351..>2099
                     /locus_tag="VFC06_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZL81815.1"
                     /translation="MSDTLYDIVILGGGSGGYAAALRGAQLGMKVALIEADKVGGTCL
                     HKGCIPTKALLHAAEVADVTRESGQFGVNATFQGIDMPKVNAYKDQIVSKLHKGLQGL
                     VGHHNITYVTGWGTLVAPDTVDVGGTPYRGRNVVLASGSYARSLPGLEIGGRVITSDQ
                     ALQMDWIPTSAIVLGGGVIGVEFASVWKSFGVDVTIIEALPHLAPNEDEALSKALERA
                     FRKRRINFKLGARFAGVQRDDNGVVVTLEDGS"
BASE COUNT          317 a          754 c          732 g          296 t
ORIGIN      
        1 ggcgtggggt acgacgtcgt cgtcttcacc ggcgtgggcg cgggcggggc caccgtggaa
       61 cgtctacggc aggccgccgg cgccgcgacg cgctgcctcg cgggcgtgtc gcgcctcgat
      121 tcggcgctgc cggccgactt ccccgagctc gtcggctccc tcacggaggg tttcgccctg
      181 ggggcctacc gtttcgaccg gtaccgtact gccgagtccg ccgtgggcac ggtacgcatt
      241 ctcaccccgc tcgcaacgga cccgctcgcg gcggccgcgg tggagcgcgg gctcatcctc
      301 gcctacgcgg tcaacaccgc acgcgaactc gtgaacacct cccccgctga cctctacccc
      361 gagacgctcg ccgacgcggc ggtccgcctc gcggccgagc acggcctcga ggcgaccgtc
      421 ctggccgtgc ccgagctgga gaagggcggc ttcgggggaa tcctcgctgt tggccagggg
      481 tccgtccgcc ccccgcgcct ggtgaaactc gtccaccgcc ccgccggcgc gaccgcccac
      541 atggcactcg tcggcaaggg catcacgttc gactccggtg ggctctccct caagccggcg
      601 aagggcatgg gcgacatgaa gtcggacatg gcgggggccg ccgcggcgct gggcgccgtc
      661 gtcgccgcgg cgcggctcgg cctcccggtc cgcgtcacgg tgtggctcgc gctcgccgag
      721 aacatgccgt ccgggtcggc gacccggccc tcggacgtca tcaccatgcg gggcgggcgg
      781 acggtcgagg tgctgaacac ggacgccgag ggccgcctgg tcctggccga cgccctgttg
      841 gcggccacgg aggaggaccc cgacgtcgtc gtcgacatcg ccacgctcac cggcgcacag
      901 atggtggcgc tcgggaaccg ggtgagcgcg gtgatgggcg atgacgacac ccgctccgac
      961 gtcctcgccg ccgccgacgc cgccggcgag caattctggc cgatgccgct gccggccgag
     1021 ctggccgagt cgctgaagac gccgatggcc gacatcgcga acatcggcga gcgcaacggc
     1081 gggatgctgg tggcgggact cttcctccag agcttcgtgg agggccgccg ctgggcgcac
     1141 ctcgacatcg ccggtcccgc gctcaacacc cagaaggcgt ggggctatac cccgatgggc
     1201 ggcaccggcg tcggggtccg caccctcgtc aacctgctcg aggccgcgac gccgtaatcc
     1261 acccgcgatc ctgcgcacgc gggtgcaggc gtgaaacaat cggactaggc gtgcaatgga
     1321 atgcaacccc cacatccgag gaggaaatac gtgagcgata ccctgtacga catcgtcatc
     1381 ctgggagggg ggagcggggg ctacgcggcc gcgctgcgtg gtgcgcaact gggaatgaag
     1441 gtcgccctca tcgaagcgga caaggtgggc ggcacctgcc tccacaaggg ctgcatcccc
     1501 acaaaggccc tgctgcacgc cgccgaggtt gccgacgtca cgcgtgagtc cggccagttc
     1561 ggcgtgaacg cgaccttcca gggcatcgac atgcccaagg tgaacgccta caaggaccag
     1621 atcgtcagca agctccacaa gggcctccag ggcctcgtcg ggcaccacaa catcacctac
     1681 gtgaccggct ggggaacgct cgtcgccccg gacaccgtgg acgtgggcgg cacgccgtac
     1741 cgcggcagga atgtggtcct cgcttccggt tcctacgccc ggagcctgcc cggcctcgag
     1801 atcggcggcc gggtcatcac gagtgaccag gccctccaga tggactggat ccccaccagt
     1861 gcgatcgtgc tcggcggggg cgttatcggt gtggagttcg cgagcgtctg gaagtccttc
     1921 ggtgtggacg tcaccatcat cgaggcgctc ccccacctcg ccccgaacga ggacgaggcg
     1981 ctgagcaagg ccctcgagcg cgcgttccgg aagcgcagga tcaacttcaa gctcggcgcc
     2041 cgcttcgccg gcgtgcagcg ggatgacaac ggcgtcgtcg tcacgcttga ggacggctc
//
LOCUS       DASRKH010000485         2348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_56762, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000485 DASRKH010000000
VERSION     DASRKH010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56762"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VFC06_07790"
     CDS             complement(<1..555)
                     /locus_tag="VFC06_07790"
                     /inference="COORDINATES: protein motif:HMM:NF021487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HZL81816.1"
                     /translation="MTDELDKDAIISLLSELGGRLHAKGVDARFYVVGGAAMLLAYGR
                     ETMTRDIDAIFEPKTVVYEEARLMASEREGLGEDWLNDGVKGFVRGPDPGVPEVVLAA
                     PGVSVEVASPKRLLAMKVAAARVGRDVEDILLLAREVGVSSIDEVLDIAFAEYGNLLE
                     ARSKFVVIEALQDVLPQHPEPTGLL"
     gene            complement(552..1124)
                     /locus_tag="VFC06_07795"
     CDS             complement(552..1124)
                     /locus_tag="VFC06_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81817.1"
                     /translation="MKTDVGAIRATSRSAAAALAAHHSAIAAALLEGARQADIAEAAG
                     VSQSAISQAMKRRRTARIGSRWTIPVLAAHLKDNPQFSLNEVVRACAQFSTDFRALAD
                     LADRQIALGAPEKIGVEHLDALVAGLADYEAWRAKISSPDWTLSSRYSMFPSWFAAPT
                     DGLKAWVMRHTPPQFAVRGVFIDERDLESV"
     gene            complement(1182..1763)
                     /locus_tag="VFC06_07800"
     CDS             complement(1182..1763)
                     /locus_tag="VFC06_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81818.1"
                     /translation="MVDHEASIALIAKSAAFLAANHLQGAATSGSVHLGLTDSTGTLV
                     AVLSASTSRTRATILRYATSCIIPGGFARLEAALVAELPPNVSTLVMHVDVATGEAGP
                     DGSDGWTSTGGAKPDYSYVVRDTRVHKSTYSQKRFRDDVALDHAEGLTVHELAALNGL
                     PRIWDAGMVRWVKPVAAAVALRTVTPDSLDLAA"
     gene            1752..2273
                     /locus_tag="VFC06_07805"
     CDS             1752..2273
                     /locus_tag="VFC06_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZL81819.1"
                     /translation="MVYHSNSELLRSVMSFTGTTQVVLSRISGVHQPTISQMLSGKRG
                     MSDRMLERLLGCMGHRPEVVRDSTLVDMNRSERRSWLLHRHLSTTMNIGTFREWKTTI
                     QRNLDHLSTGTSGQPHERNLHTWRSLVDSGDIEGIRRILTGVDRDSIEMREVSPMNGL
                     IHESERQDILMAA"
BASE COUNT          458 a          768 c          725 g          397 t
ORIGIN      
        1 gagtagcccc gtcggctctg ggtgctgcgg gagcacgtcc tggagtgctt cgatcacaac
       61 gaacttgctg cgggcttcga gcaggttccc gtattcggca aacgcgatgt cgagaacctc
      121 gtcgatcgac gacacaccca cctcacgagc gagaaggaga atgtcctcga catcgcgacc
      181 aacgcgcgca gccgcaacct tcatcgccag gaggcgcttg ggggacgcca cttcgaccga
      241 gactccgggt gccgcaagca ccacctccgg cacgccaggg tccggtccgc gaacgaaccc
      301 cttgaccccg tcgttcagcc agtcctctcc cagcccctcc cgctcagaag ccatgagacg
      361 agcctcttcg tagacgacag tcttcggctc gaagatggca tcgatgtcgc gcgtcatggt
      421 ctcgcggccg taggcaagca gcatcgccgc tcctccaaca acgtagaagc gtgcatcaac
      481 ccccttcgcg tgaagtcgtc cgccaagttc tgacagcaga ctgatgatcg cgtccttatc
      541 cagttcgtcc gtcataccga ttccaggtcc ctctcgtcga tgaacactcc cctgaccgca
      601 aactgcggcg gagtatggcg catgacccac gccttgagcc cgtcagtcgg agcagcgaac
      661 cacgacggga acatcgagta cctggacgac aacgtccagt ccggagatga gattttggcc
      721 ctccacgctt catagtcggc gaggccggcc acgagcgcgt caaggtgctc aacgccaatc
      781 ttctctggcg cgccgagagc aatctgcctg tccgccagat ccgcgagcgc ccgaaagtcc
      841 gtggagaact gcgcacaagc gcgcacaacc tcgttcaaag agaactgcgg gttgtctttc
      901 agatgcgcgg caagaacggg aatcgtccag cgcgagccga tgcgagcagt gcgccgccgc
      961 ttcatggcct gagagattgc ggactggctg acacccgcag cctccgcaat gtcggcctgc
     1021 cgcgcccctt ccagcagcgc ggcagcgatc gccgagtggt gagcggcgag tgccgcggcg
     1081 gcggacctcg aagtggcgcg aatggcgccg acgtcagtct tcatataagc caccttatat
     1141 caagtccgcg ttagtgtcca tttgccgcgc acacacggac gctacgccgc gaggtcaagc
     1201 gagtcagggg tgaccgtgcg cagcgcgacc gcggcggcga cgggcttgac ccagcggacc
     1261 atccccgcgt cccaaatgcg gggcaggccg ttcagggcag caagttcgtg caccgtcagg
     1321 ccctcggcgt ggtcgagagc cacgtcgtca cggaaacgct tctgcgagta ggtggacttg
     1381 tggacgcggg tatcgcgcac gacgtacgag tagtcgggct tggcgcctcc ggtgctggtc
     1441 cacccgtcgg acccatccgg ccctgcttct cccgtggcga cgtctacgtg catcacgagg
     1501 gtcgacacgt tgggtggcag ttcggcgacg agcgccgctt cgagccgcgc gaagcctccg
     1561 gggatgatgc aggacgtggc gtaacggagg atagtcgcgc gggtcctcga agtcgacgcc
     1621 gacagaacgg cgaccagggt gccggtggag tcggtcaggc ccaggtggac gctgccggac
     1681 gtggctgcgc cttggaggtg attggcggcc aggaaggcgg cggacttagc aattaatgct
     1741 atactcgctt catggtctac cacagcaact ctgaacttct tcgcagcgtg atgtccttca
     1801 ccggcaccac gcaggttgtg ctgtctcgaa tcagcggcgt tcaccagccc actatcagcc
     1861 agatgctttc tggcaagcga ggcatgagcg acaggatgct cgagcgcctc ctgggctgca
     1921 tgggccaccg ccccgaggtg gtgcgtgact cgaccttggt ggatatgaac cgctcggagc
     1981 gccgctcatg gctactgcac cgccacctgt ccaccaccat gaacatcggc accttccgcg
     2041 agtggaagac gaccatccag cgcaaccttg accacttgag cacaggaacc agtggtcagc
     2101 cgcatgagcg caacctgcac acctggcggt cattggtgga ctccggcgac atcgagggta
     2161 tccgacgcat cctcaccggg gtagatcgcg actctatcga gatgcgggaa gtctccccga
     2221 tgaatggact gatccacgaa agcgaacgcc aagacatcct gatggctgcg tagcgcggtg
     2281 cgacgcgagc aactggagca cgccattcgg gccgcgtgcc aaatcaccgg cactcagcga
     2341 gtcatcat
//
LOCUS       DASRKH010000486         2326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_55924, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000486 DASRKH010000000
VERSION     DASRKH010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55924"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VFC06_07810"
     CDS             complement(<1..710)
                     /locus_tag="VFC06_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HZL81820.1"
                     /translation="MMFGEVPPAQEVDSARRIMGLETEFGLHFDAPNSRAVTPEEVAG
                     HLFHSVVEWGRSSNVFLTNGSRLYIDVGAHPEYATAECDSLTDLVAYDKAGERIVHAL
                     VAKGEARLASAGLEGSIYLYKNNTDSAGNSYGCHENYLVRRRADFKAFASVLLPFFVS
                     RQIVTGSGCITRTPQGSHYSFSPRADHMWEGMSSATTRSRPMINTRDEPQAHADLYRR
                     MHVIVGDSTMTEPTTRLK"
     gene            complement(717..902)
                     /locus_tag="VFC06_07815"
     CDS             complement(717..902)
                     /locus_tag="VFC06_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012245440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HZL81821.1"
                     /translation="MPQTTSSSSPFEDDAPEPDAPSAPAAQVSTDALDALLDEIDDTI
                     QTNATQFVRSFVQKGGQ"
     gene            complement(912..>2326)
                     /gene="dop"
                     /locus_tag="VFC06_07820"
     CDS             complement(912..>2326)
                     /gene="dop"
                     /locus_tag="VFC06_07820"
                     /EC_number="3.5.1.119"
                     /inference="COORDINATES: protein motif:HMM:TIGR03688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="depupylase/deamidase Dop"
                     /protein_id="HZL81822.1"
                     /translation="GGEDPLMDLRGMRRDRRYATADLLTDSPEYSDANRGVTLRRRRG
                     SWEDPAHLNAVLANGARFYVDHAHPEYSGPEVTSAHDAVVWDVAGDRIAAAAAAHMRA
                     GGDDVALYKNNVDGKGASYGTHENFLVQRSVDFDDLARRLIPHLVSRQVVVGAGRVGL
                     GQHGQEAGFQISQRADYIEAEVGLETTLRRPIVNTRDEPHADRRKWRRLHIILGDANC
                     MEVPTYLKVGTTALVLSAIEAGDERLDVLALADSVAEVGAVSRDLTLRHRIRLASGES
                     ATALEIQRALVTIARDYVSDEEDARVIARWERVIDALARDPFEAARDVEWVAKLQLFG
                     RMRERYATVDADGARTLPDWNDDRLRAADLQWSELDTGLAARLSAAGAVERVASDARI
                     EEAMTTPPSTTRAWLRGRMVSQRSDLVDAASWSTIVLDRDAEHGHLEVLTLPDPLVNS
                     HHLVDTLLAEAGPADARTRE"
BASE COUNT          380 a          767 c          768 g          411 t
ORIGIN      
        1 atcttgaggc gcgtggtcgg ctccgtcatg gtcgagtcgc cgacgatcac atgcatccgc
       61 cgatacaggt cggcgtgcgc ttggggttcg tcgcgcgtat tgatcatggg gcgcgaacgc
      121 gtggtggcgg aactcattcc ctcccacatg tggtcggcgc gtggggaaaa gctgtagtgg
      181 gagccttgcg gcgtgcgcgt gatgcagccc gagcccgtga cgatctgccg cgacacaaag
      241 aacggcaaca gcaccgaggc gaaggccttg aagtccgcac gcctgcgcac caggtaattc
      301 tcgtggcagc catagctgtt gcccgccgag tcggtgttgt tcttgtacag gtagatcgag
      361 ccctccagcc cggccgacgc gagacgggcc tcgcccttcg cgaccagggc gtggacgatg
      421 cgctcacccg ccttgtcata cgcgacgagg tcggtaagcg agtcgcattc ggccgtcgcg
      481 tattcggggt gcgcgcccac gtcgatgtag agccgcgaac cgttggtgag gaacacgttg
      541 gaggaccggc cccactcgac gactgagtga aacagatgcc ccgcgacctc ttccggcgtg
      601 accgcgcgtg agttcggggc gtcgaagtgc aggccgaact cggtctccag gcccatgatt
      661 ctgcgcgccg aatcgacctc ttgggccggc ggcacttcgc cgaacatcat gtgctgctac
      721 tgcccgccct tttgcacgaa ggaccgaacg aattgcgtgg cattcgtctg aatcgtgtcg
      781 tcgatctcgt cgagcaaggc gtcgagcgca tccgtgctca cctgcgcggc tggcgcggag
      841 ggagcgtctg gctctggggc gtcgtcctcg aacggggagg acgatgaggt ggtctgaggc
      901 atgctcctag cctactcgcg cgtgcgcgcg tccgcgggcc ccgcctcggc aagcaaggtg
      961 tcgacgaggt gatgactgtt gacgagcggg tcgggcagcg tgagtacttc gagatggccg
     1021 tgctcggcgt ccctgtccaa cacgatcgtc gaccagctgg cggcatcgac gaggtcgctg
     1081 cgttggctca ccatgcggcc tctcagccat gcacgagtgg tggagggagg agtggtcatg
     1141 gcctcctcga tgcgagcgtc cgacgcgaca cgctcgacag cgcccgcagc gctcagtcgc
     1201 gccgcgaggc ccgtatccag ttcgctccac tgcagatccg cggcgcgcag gcgatcgtcg
     1261 ttccagtcgg gaagcgtccg cgcaccgtcg gcgtcgaccg tggcgtagcg ctcgcgcatg
     1321 cggccgaaca gttgcagctt cgccacccac tccacgtcac gcgccgcctc gaatgggtcc
     1381 ctcgcgagcg cgtcgatgac tcgctcccag cgcgcgatga cacgcgcatc ctcctcgtcg
     1441 ctcacgtagt cgcgagcgat cgtcacgagt gcccgctgaa tctccagagc ggttgccgac
     1501 tcgccggacg cgagacggat ccgatggcgc agcgtgaggt cgcgggacac ggcccccacc
     1561 tctgccacgg aatcggcaag tgcgagaacg tcgagccgct catcccccgc ctcgattgcc
     1621 gacaggacca acgccgtcgt gcccaccttc agataggtag gcacctccat gcagttcgcg
     1681 tcgccgagaa tgatgtgcag gcgacgccac ttgcgtcgat ccgcatgggg ctcatcgcgg
     1741 gtgttgacga tcggcctgcg cagtgttgtc tcgagcccca cttcggcctc gatgtagtcg
     1801 gcacgctggg agatctggaa acctgcctct tggccatgct gaccgagacc gacacgaccc
     1861 gcgcccacca ccacttggcg gctcaccagg tgagggatga gtctgcgcgc gaggtcatca
     1921 aagtcgaccg agcgttgcac aaggaagttc tcgtgggtgc catacgaggc gcccttgccg
     1981 tccacgttgt tcttatacag cgcaacgtcg tcgccaccgg cgcgcatgtg ggctgctgcc
     2041 gccgcagcga tgcggtctcc cgcaacatcc cacaccaccg catcgtgggc gctcgtcacc
     2101 tcggggccag agtattcggg atgagcgtgg tcaacataga agcgcgcgcc attggccaac
     2161 actgcgttca agtgggccgg gtcctcccac gagccacggc gccttcgcag ggtgactccc
     2221 cgattggcat cggagtattc cgggctgtcg gtaaggaggt cggccgtcgc atagcggcgg
     2281 tccctgcgca tgcctcggag gtccatcaac gggtcctcgc cgccgt
//
LOCUS       DASRKH010000487         2627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_61408, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000487 DASRKH010000000
VERSION     DASRKH010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61408"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /locus_tag="VFC06_07825"
     CDS             complement(<1..992)
                     /locus_tag="VFC06_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HZL81823.1"
                     /translation="MTHSDTGGTRLDPWLGSYAARAHNMRSSEIRALFAVASRPEVVS
                     LAGGMPFIGGLPLEEIGEMMRRLVAEQGEVALQYGSGQGYEPLREQITQVMALEGIHA
                     HPDDVVVTTGSQQALDLVTKIFIDPGDVILAEAPSYVGALGVFRAHEADVVHVAMDED
                     GLVPEALESTLTEVVTSGRRVKFLYTVPNFHNPGGVTLSLERRPRILEICRRFGVLVL
                     EDNPYGLLGFDAEPLPALRSMDEEGVIYLGSFSKTFAPGFRVGWALAPHAVREKLVLA
                     SEAAVLCPSNASQMAISSYLEHHDWQGQIKKFREQYRERRDAMISALEEFMPEAS"
     gene            complement(1056..1382)
                     /gene="trxA"
                     /locus_tag="VFC06_07830"
     CDS             complement(1056..1382)
                     /gene="trxA"
                     /locus_tag="VFC06_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HZL81824.1"
                     /translation="MSIIVNATEDTFKAEVLESPMPVVVDFWAEWCGPCRAVAPILEE
                     LSGEYVGKLKIVKVDTEAAPGIAMAYGITSIPTMHFFKNGEVVKTLVGARPKAALQAQ
                     FDEIVG"
     gene            complement(1382..2362)
                     /gene="trxB"
                     /locus_tag="VFC06_07835"
     CDS             complement(1382..2362)
                     /gene="trxB"
                     /locus_tag="VFC06_07835"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HZL81825.1"
                     /translation="MTEIRNVVIVGSGPSGYTAAIYAARAGLNPLVIAGSVTAGGALM
                     NTTEVENFPGFVDGIQGPELMQTLQGQAEKFGAEVLFDDATQLELEGPIKRITTGMGK
                     VFETRAVILSMGSAYREIGIDDEKRLSGHGVSWCATCDGFFFRNQDVMVVGGGDSAME
                     EATFLTRFANKVTVVHRRNELRASKVMADRAKADPKIEFAWNSAVTGLEGEAKLSAVQ
                     LTDMITGEVRTVPATGLFVAIGHDPRSELVKGVVELDAAGYVNVDGGTTLTNIPGVFA
                     AGDLVDSRYRQAITAAGTGCAAALDAQRWLEEIEDSQHGLDQATTVEGGV"
     gene            complement(2448..>2627)
                     /locus_tag="VFC06_07840"
     CDS             complement(2448..>2627)
                     /locus_tag="VFC06_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81826.1"
                     /translation="FNVGPLLVASLIVLLFVAAVIGASMITRPIDAPFDGYNNPSQTY
                     PEYTFGQTPPPTPEG"
BASE COUNT          459 a          857 c          851 g          460 t
ORIGIN      
        1 gaggcttcgg gcatgaactc ctcgagagcc gagatcatgg cgtcacgccg ctcgcgatac
       61 tgctcccgga acttcttgat ctggccttgc cagtcgtgat gctcgagata gctcgagatt
      121 gccatttgcg aagcgttgga cggacagagg acggccgcct ccgacgcgag caccagcttc
      181 tcgcgcacgg cgtgtggggc cagcgcccag cccacacgga agccaggcgc gaaggtcttg
      241 gagaaagacc ccaggtaaat gacgccttct tcgtccatgg agcgtagcgc cggcagcggc
      301 tcggcgtcga aaccgagcag cccatagggg ttgtcctcca gcacgagcac gccaaagcgt
      361 cggcagatct ccaagatgcg ggggcgccgt tccagggaca gcgtgacgcc gccagggttg
      421 tggaagttcg gcaccgtgta gagaaacttc acgcggcgcc ctgatgtcac gacttcggtg
      481 agcgtggact cgagcgcctc cggcaccagg ccgtcctcgt ccatggcgac gtgcaccacg
      541 tcggcctcgt gggcccggaa cacgcccaag gcgcccacgt aggacggggc ctctgccaga
      601 atgacgtcgc ccgggtcgat gaagatcttg gtgaccaggt cgagcgcctg ctgtgagcca
      661 gtggtgacca ccacatcgtc cgggtgcgcg tgaatgcctt cgagcgccat gacctgggtg
      721 atctgctcgc gcaagggctc gtatccctgg ccggaaccgt attggagcgc cacttcgccc
      781 tgctcggcga cgaggcggcg catcatctcg ccgatctctt ccaggggcag tccgccgatg
      841 aacggcatgc cccccgcgag cgacaccacc tccgggcgtg acgcaacagc aaacagggcg
      901 cgaatctccg acgaacgcat gttgtgcgcc cgggcggcgt acgaccccaa ccaagggtcg
      961 agcctggtac cgccggtgtc cgagtgcgtc atgggcgcca gtctttcacg cctgccgctg
     1021 agtgcgcgcg ctccagtcgc tcgcggctgg agcccttagc cgacgatctc gtcgaactga
     1081 gcctggagcg cggccttcgg ccgagcgccc accagggtct tcaccacttc gccgttcttg
     1141 aagaagtgca tcgtgggaat cgacgtgatg ccgtacgcca tcgcgattcc cggggcggcc
     1201 tcagtgtcca ccttgacgat cttgagcttg ccgacgtact caccggacag ctcttcgaga
     1261 atgggcgcga cggcgcggca ggggccgcac cattccgccc agaagtccac gacgacgggc
     1321 atgggggact ccagcacttc ggccttgaac gtgtcttccg tggcgttgac gatgatgctc
     1381 atcacacgcc tccttccact gtggtggcct ggtccaggcc gtgttgtgag tcttcgattt
     1441 cttcgagcca gcgttgcgcg tcgagagcgg ccgcgcaccc ggtgcccgca gcggtgatcg
     1501 cctgacggta gcggctgtcc acgaggtctc ccgcggcgaa cacgccgggg atgttcgtga
     1561 gcgtggtgcc gccgtcgaca ttgacgtatc cggccgcgtc caactcgacg acgcccttca
     1621 cgagttccga gcgcgggtcg tgcccgatcg cgacaaacaa cccggtggcg ggaacggtgc
     1681 gcacctcgcc cgtgatcatg tcggtcaact gaaccgcgga gagcttggcc tcaccctcga
     1741 ggcccgtcac cgccgagttc cacgcgaact cgatcttggg gtcggccttg gcgcggtccg
     1801 ccatcacctt ggacgcgcgc aattcgttgc ggcggtgaac cacggtcacc ttgttggcaa
     1861 agcgcgtgag gaaggtggcc tcctccattg cggagtcgcc gcctccgacc accatgacgt
     1921 cttgattgcg gaagaagaag ccgtcgcagg tcgcacacca gctgacgccg tggcccgaca
     1981 ggcgcttctc gtcatcaata ccgatctcgc ggtaagcgga acccatcgac agaatgacgg
     2041 cgcgggtctc gaagaccttg cccatgcccg tggtgatgcg cttgatgggt ccctcgagtt
     2101 cgagctgcgt ggcgtcgtcg aagagcacct cagcgccaaa cttctcggcc tgtccctgaa
     2161 gcgtctgcat cagttcgggt ccctggatcc cgtcgacaaa gccggggaag ttctccacct
     2221 cggtggtgtt catcagcgcg ccgccggcgg tgacggagcc cgcgatgacg agcgggttca
     2281 accccgcccg ggcggcgtag atggcggcgg tatagccgga ggggccggag cccacgatca
     2341 cgacgttgcg aatctcagtc acgaatcaaa cctctgtttc agatggtgat agcccaacac
     2401 gatcgtaggc ctgtcgtatt ccatccgccg catggcgcgg cggatggtca tccctccggc
     2461 gtcggagggg gcgtctgccc aaacgtgtat tcggggtacg tctgcgaagg gttgttgtaa
     2521 ccgtcgaatg gtgcgtcaat cggccgggtg atcatcgacg cgccgatcac ggcagcgacg
     2581 aacagcagca cgatgagtga ggccaccaac aaggggccca cgttgaa
//
LOCUS       DASRKH010000488         2159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_46901, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000488 DASRKH010000000
VERSION     DASRKH010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46901"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..162)
                     /locus_tag="VFC06_07845"
     CDS             complement(<1..162)
                     /locus_tag="VFC06_07845"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZL81827.1"
                     /translation="MSRPVHFEIQADDIERAKAFYAAAFGWTFEDYTAFVDSPYWGVI
                     TGAEGEPGIN"
     gene            complement(202..597)
                     /locus_tag="VFC06_07850"
     CDS             complement(202..597)
                     /locus_tag="VFC06_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81828.1"
                     /translation="MATLGANIPAIVVFTVVGVAWSVVGLLVIAPRVFPEHWFENALA
                     DFGQSQGNVATGFVPCDMADRRRQSGAARAYGYKQLTYEPILGGGIITALSVPFITDV
                     GLVPFTVVSAVIAVAMVVWGVMRNRSRGA"
     gene            complement(1036..1287)
                     /locus_tag="VFC06_07855"
     CDS             complement(1036..1287)
                     /locus_tag="VFC06_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81829.1"
                     /translation="MLAKYTATQVTGLALVVLTFVIFLAWVLRRRVTFFQRLYIPTAV
                     IGGFLMLLLGPQVLGALTNSQGASSRTGSSTSGVCSPAS"
     gene            complement(1386..1571)
                     /locus_tag="VFC06_07860"
     CDS             complement(1386..1571)
                     /locus_tag="VFC06_07860"
                     /inference="COORDINATES: protein motif:HMM:NF036450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5302 domain-containing protein"
                     /protein_id="HZL81830.1"
                     /translation="MAEKKSNPAADEIKSKMREALDRKQGKEREARDGAAGNGAQKPH
                     GVDHPVGHKEFRRKSGG"
     gene            1679..>2159
                     /locus_tag="VFC06_07865"
     CDS             1679..>2159
                     /locus_tag="VFC06_07865"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZL81831.1"
                     /translation="MDHDAAPTRRALAAVRTAPTILEAIRRADDLIDVAAREASPGEV
                     VRLLREALVDASDASDALTAIAAVHALAAVRDSGGAAETLVGLLSDGTPNLREHAAWA
                     LGSSPAAPRAIPELEAMVSGGGFGGTLAQLTLEAWGVGGDETLGLVPGGTTTAASSTQ
                     "
BASE COUNT          340 a          738 c          759 g          322 t
ORIGIN      
        1 gttgatcccc ggctcaccct ccgctccggt gatgactccc cagtagggcg agtcgacgaa
       61 ggcggtgtag tcctcgaatg tccagccgaa ggcggcggcg tagaacgcct tcgcgcgctc
      121 gatgtcgtcg gcctggatct cgaagtggac ggggcgtgac atgggaactc ctcaaggcgg
      181 cggcgtgtgg tctgtcacat cctaagcacc acgcgacctg ttgcgcatga ccccccacac
      241 caccatggca accgcgatca cggcggacac gacggtgaac ggcaccagcc ccacgtccgt
      301 gatgaacggg acggagaggg ccgtgatgat gccgccgccg aggatcggct cgtaggtgag
      361 ttgcttgtac ccgtaggctc tggcggcgcc gctctggcgg cgccggtcgg ccatgtcgca
      421 aggcacgaag ccggtggcga cgttcccctg ggactgaccg aagtccgcca gtgcgttctc
      481 gaaccagtgc tcggggaaca cgcgcggcgc gatgaccagc agtccgacca cgctccacgc
      541 cacgccgacc acggtgaaca ccacgatggc ggggatgttt gcgccgaggg tggccaacga
      601 ctggggagat cctgacctcg ttgaggtcgg tgtgctcgga ctcagccatc ggggtctgcc
      661 ggctgacggg gatcttcggc gacttcatgg cgaagttgac gagcgcggtc ccaaccacca
      721 cgccggtcac catcgagatc gtggcgaggc ccaagccgat atcgacgacc tcgggggcgc
      781 cgacgtcgta gagcagttgg gtcatgccgg cgaccgtgcc gtggccgccg gcgaaggaca
      841 tctcgatggc ggcgccggcc gccggcgaca gcccgaacac cggaccgagg atgaggagca
      901 ccgcgagccc accgagcgcg aactggtcga agggaaaggt gctgcccatg atgaagtgcg
      961 gggccgactt ctcccacacc tccttgagcg gcggaacgcg cttgccgatc gtgatcgcgc
     1021 cgaacacgat gttgatcagg atgccgggga gcacacgcca gacgttgatg agcccgtccg
     1081 ggaagaggcc ccctggctgt tggtgagggc gccgagcacc tgcggtccga gcagcaacat
     1141 caggaacccg ccgatcacgg cggtgggaat gtagaggcgt tggaagaacg tcacgcggcg
     1201 ccgcaacacc cacgccagga agatcacgaa cgtgagcacg acgagcgcca gcccggtgac
     1261 ctgggtggcg gtgtacttgg ccaacatggc gggctcctgt ggacccccac gctaccgggc
     1321 catggcgcgc gccgacacac cgcggctgag cccttccccg agtctcagcc ggtcttccgc
     1381 gagtctcagc cgccggactt gcggcggaac tccttgtggc ccaccgggtg gtccacaccg
     1441 tgcggcttct gggcgccgtt gccggcggcg ccgtcgcggg cctcacgctc cttgccctgc
     1501 ttgcgatcca gggcctcgcg catcttgctc ttgatctcgt ccgcggcggg gttcgacttc
     1561 ttctcggcca tggtgttcct ctcgtaaggg tcaatcatcg ctgctcgggg cgtccggggc
     1621 cacccatttc ccggtgagcc ccgggcggcc gccctccccg ggacctagca tgagtgacat
     1681 ggaccacgac gcggcgccaa cccgtcgagc gctcgctgcc gtgcggaccg cgcccaccat
     1741 cctggaggcc attcgccggg ccgatgacct gatcgacgtc gccgcgcggg aggcctcacc
     1801 cggggaagtg gtccgtctcc tgcgggaggc gctcgtcgac gcctcggacg cctcggacgc
     1861 cctgaccgcg atcgccgcgg tccacgcgct ggccgccgtg cgggacagcg gcggggcggc
     1921 ggaaaccctg gtgggcctcc tctcggacgg cacccccaac ctgcgtgaac acgccgcgtg
     1981 ggcgctcggc tcgtcccccg cggcgccacg ggccataccg gaactcgagg cgatggtgtc
     2041 gggcgggggt ttcgggggca cgctggccca actcaccctt gaggcgtggg gtgtcggtgg
     2101 ggacgagacg ctcgggctgg tgccgggtgg gacgacgacg gcagcctcca gcacgcagg
//
LOCUS       DASRKH010000489         3260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_43479, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000489 DASRKH010000000
VERSION     DASRKH010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43479"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /gene="hisD"
                     /locus_tag="VFC06_07870"
     CDS             <1..821
                     /gene="hisD"
                     /locus_tag="VFC06_07870"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HZL81832.1"
                     /translation="PHPTILAACALLGVTEVYAVGGAQAIAMLAYGAEATAGGTLCEP
                     VDVITGPGNVYVAAAKRAVNGTVGIDSEAGPTEIAIIADDAADPTFVAFDLLSQAEHD
                     PMAGSVLITDSAELAEAVGEKLTSIVGATKHDVRAKEALGGVQSAIILTDNLDQSIRV
                     ADAYGAEHLEIHTRDAAAVAGRIRNAGAIFVGPHSPVPLGDYLAGSNHVLPTGGTSRF
                     ASGLGVTSFLRAVSVIEYDAAALAAVGDKVIALANAEDLPAHGEAIAARLHPAD"
     gene            884..1978
                     /locus_tag="VFC06_07875"
     CDS             884..1978
                     /locus_tag="VFC06_07875"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HZL81833.1"
                     /translation="MTLPLRPELEGLTPYGAPQLDVAAMLNVNENPYPPPPSVVAAIT
                     AAVRQAAGGLNRYPDREFMALRADLARYLALESRVDFLEPKNIWAANGSNEIMLHLHQ
                     AFAGPGRKVLSFAPTYSMYPEYSRDTVTEYVTLPRRDDFRLDMDAAVAAIADIKPTIV
                     LVASPNNPTGTALGLDEVIALADACAKVPGGSVLVVDEAYAEFRRRGVPSAATLLPEI
                     PHLVVTRTMSKAFALAGGRLGYLAASKEVVDALRIVRLPYHLSAITQAAARAALAHHR
                     ELQGQVDEIRAERDDTVDWLRAQGYTVPDTDANFVLFGPVPDREEVFEGLLARGVLIR
                     VVGPEGWLRVSIGTAREMAMFRNGLLEVTA"
     gene            1975..2604
                     /gene="hisB"
                     /locus_tag="VFC06_07880"
     CDS             1975..2604
                     /gene="hisB"
                     /locus_tag="VFC06_07880"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011774316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HZL81834.1"
                     /translation="MTDTTGARTTPARTGRVERATSESSVLVELDLDGSGTTEISTGV
                     PFYDHMLTALGKHSLMDLKVQATGDVHIDSHHTVEDVAICIGEALKQALGDKAGISRF
                     GDAMVPLDEALAQCVVDVSGRPYFVHTGEPAGQATHLIGGNFAGSLTAHVLESIAHHA
                     GICVHMRVLAGRDPHHIVEAQFKALARALRAAVSPDARVKGIPSTKGAL"
     gene            2601..3098
                     /locus_tag="VFC06_07885"
     CDS             2601..3098
                     /locus_tag="VFC06_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZL81835.1"
                     /translation="MTTEEALDGAPAQIAALFTPIPNGRVLAALCALNGIKAQVLETS
                     AGAIAVLDDATPVSLHGAAHSVSSFAKERPLVAMERRDGQITVWKYSAGQRGETLPPG
                     LALNDAPGVVTTLMTGAQTIADVAVTHEDKVFDAHMGKVAAFRELRKLSTQAKREAKA
                     QRDGR"
     gene            3147..>3260
                     /locus_tag="VFC06_07890"
     CDS             3147..>3260
                     /locus_tag="VFC06_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HZL81836.1"
                     /translation="MPTVTVLDYGFGNVRSAVRALEHVGADVTLTADRAAAE"
BASE COUNT          543 a         1097 c         1103 g          517 t
ORIGIN      
        1 ccccgcaccc gaccatcctt gcggcgtgcg cgctgttggg cgtgaccgag gtgtacgcag
       61 tcggcggtgc ccaggcgatc gcgatgctgg catatggagc agaggccacc gcgggcggca
      121 ccctgtgtga gcccgtggat gtcattacgg gtcctggcaa cgtctacgtg gcggccgcga
      181 agcgcgcggt caacgggacc gtgggcatcg attccgaggc gggccccacg gagatcgcca
      241 tcatcgccga cgacgcggcc gaccctacgt ttgtggcctt tgaccttctc agccaagcag
      301 agcacgaccc catggcgggc tcggtgctca tcacggacag cgctgagttg gcggaagcgg
      361 tcggggagaa gttgacgagc atcgtgggcg ccaccaagca cgacgttcgc gcgaaagagg
      421 cgctcggcgg cgtgcagagt gcgatcattc tcaccgacaa cctggaccag tccattcggg
      481 tcgccgacgc ctacggcgcc gaacacctcg agatccacac ccgtgacgcg gccgccgtgg
      541 cgggccgcat tcgcaacgcg ggtgccatct ttgtgggccc gcacagcccg gtgccgctgg
      601 gcgactatct ggcaggctcc aaccacgtgt tgcccacggg cggcacgtcg cgattcgcta
      661 gcgggctggg cgtgacgagc ttcctgcgag cggtctcagt gatcgagtac gacgccgctg
      721 ccctggcggc cgtgggcgac aaggtgatcg ccctcgcgaa cgccgaggac ctgcctgcac
      781 acggcgaggc aattgccgcg cgcctgcacc ccgccgactg acgcatcggc ccggcactca
      841 ctgacgcatc ggcgcattgc cgcctcgcca ctagactctc ggcatgacgt tgccgcttcg
      901 ccccgaactt gagggactca cgccctacgg agccccgcaa ctggacgttg cggcgatgct
      961 caacgtgaac gagaacccct acccgccgcc gccgagcgtg gtcgcggcga tcaccgcagc
     1021 ggtgcgccaa gccgcggggg gcctcaaccg ttaccccgat cgggaattca tggcgctgcg
     1081 cgcggatctc gcgcgctatc tggcgctgga aagccgcgtc gacttccttg agccgaagaa
     1141 catctgggcg gccaacgggt cgaatgaaat catgcttcac ctgcaccagg cgttcgcggg
     1201 cccaggacgc aaggtgctga gcttcgcacc cacgtattcg atgtaccccg agtacagccg
     1261 cgacacggtc accgaatacg tcaccctgcc ccgtcgtgac gacttccgcc tcgacatgga
     1321 cgccgccgtg gccgccatcg ccgacatcaa gccgacgatc gtgctggtgg cgagccccaa
     1381 caaccccacc ggcacggcgc ttggcttgga tgaggtcatc gctcttgccg atgcctgtgc
     1441 caaggttccc ggcggtagcg tgttggtggt ggatgaggcg tacgccgagt tccgtcgcag
     1501 gggagtgccg agcgctgcaa cccttcttcc agagattcct cacttggtgg tgacgcggac
     1561 gatgtcgaag gcgtttgccc tggcaggcgg cagattgggc tacctcgcgg caagcaagga
     1621 agtggtggat gcgctgcgca tcgtccgcct tccgtaccac ttgagcgcca tcacccaggc
     1681 ggccgcccga gccgcgctgg cccaccaccg cgagctgcag ggccaggtgg acgagattcg
     1741 cgccgagcgc gacgatacgg tggactggtt gcgtgcccag ggctacacgg tgcccgatac
     1801 ggacgccaac tttgtgctct tcgggcccgt gccggatcgt gaagaagtgt ttgagggcct
     1861 gctcgcgcgt ggcgtcctca tccgcgtggt cggtccggag ggctggctgc gggtgtcgat
     1921 cggcacggcc cgcgagatgg caatgttccg caacggactc ttggaggtca ccgcatgacc
     1981 gacaccactg gcgcccgaac cactccggcg cgcaccgggc gggtggagcg cgccacgagc
     2041 gaatcgagcg tgctcgtcga actcgacctg gacggctccg gcaccaccga gatcagcacc
     2101 ggcgttccgt tctacgacca catgctcacg gcgttgggca agcactccct catggatctg
     2161 aaggtccagg cgacgggcga cgtccacatt gacagccacc acacggtcga ggacgtggcc
     2221 atctgcattg gcgaggccct gaagcaggcc cttggcgaca aggcgggcat cagccgcttt
     2281 ggcgacgcca tggtgccgct cgacgaggcc ctcgcacagt gcgtcgtcga cgtgtctggt
     2341 cggccctact ttgtgcatac gggtgagccg gcaggtcagg ccacgcacct catcggcggc
     2401 aactttgccg gttcgctcac ggcccacgtc ctcgaatcga tcgcccacca cgcgggcatc
     2461 tgcgtgcaca tgcgcgtgct ggccggtcgc gatccgcacc acatcgtgga ggcgcagttc
     2521 aaggcgcttg cccgtgcgtt gcgtgccgcc gtatcgcccg atgccagggt caaggggatc
     2581 ccctccacga agggggcgct gtgaccaccg aggaagcact cgacggcgct ccggcgcaga
     2641 ttgccgcgct gttcacgccc atcccgaatg ggagggtcct ggcggcgctg tgcgcgctga
     2701 acgggatcaa ggcacaggtg ttggagacgt cggccggggc catcgcggtg ttggacgatg
     2761 cgacgcccgt tagcctccac ggcgcggccc attcagtgtc gagctttgcc aaggagcggc
     2821 cgctcgtggc gatggaacgg cgcgacggcc agatcaccgt gtggaagtac tcggcgggcc
     2881 agcgcggcga aacgctgccg ccgggattag cgctcaacga cgcacccggg gtggtgacaa
     2941 cgctcatgac gggcgcccag accatcgcgg acgttgcggt cacccacgag gacaaggtgt
     3001 ttgacgcgca catgggcaag gtcgcggcct tcagggaact gcgtaagctg tccacgcagg
     3061 ccaagcgcga ggcgaaggca cagcgagacg gccgctgagg ctgtggggca gcgggaccga
     3121 ctcctgagcg ccgttagaat cgccgcatgc ctacggtgac tgttcttgac tacggctttg
     3181 gaaacgtccg ctccgcggtg cgtgctctcg agcacgtggg cgccgacgtg accctcaccg
     3241 ccgaccgcgc cgcagcagag
//
LOCUS       DASRKH010000490         2886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_46928, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000490 DASRKH010000000
VERSION     DASRKH010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46928"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /gene="argC"
                     /locus_tag="VFC06_07895"
     CDS             <1..902
                     /gene="argC"
                     /locus_tag="VFC06_07895"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HZL81837.1"
                     /translation="PHLRALAGRVLVETTTENLSGADVVFLALPHGASGAVAAELEGA
                     VVIDCGADFRLEDAADWEAFYGSPHAGSWPYGFPELRHAGEGAAREQREILARATRIA
                     VTGCNVAAVTLALQPGVGLVDASGIVATLAVGYSGAGKAPKPHLMAAEALGSAAPYAV
                     GGTHRHIPEIEQNLRTAGALNPRVAFQPILVPMSRGILASVTAPLTGDPAEVRPAWEA
                     ACAGEPFVELLPEGEWPTTAAVAGANTALVQVAVDGHAGRVIAMCALDNLVKGTAGSA
                     IQAMNLALGLPETTGLTTVGVAP"
     gene            899..2059
                     /gene="argJ"
                     /locus_tag="VFC06_07900"
     CDS             899..2059
                     /gene="argJ"
                     /locus_tag="VFC06_07900"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002853669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HZL81838.1"
                     /translation="MSVTAPRGFRAAGVTAGLKKSGRPDLALVVNDGPLDVAAGVFTT
                     NRVQAAPVLWSRTAVGDGRACAVILNSGGANACTGSGGFLDSHTTAEHVAAVLGVGAA
                     DVLVCSTGLIGERLAMPVLLLGVDAAAAALGVTGGPDAAAAICTTDTVPKETVQRGEG
                     WSIGGMAKGAGMLNPALATMLVVLTTDAVLDAGMADAALRHAVEHSFNRLDSDGCMST
                     NDTVLLLASGAAGASPDAAVFGAALDAACADLARQLIADAEGASHDIAVTVRGATTEG
                     AALAVARAVTSSNLFKAAVFGNDPNWGRVLAAVGTVPVEVAAYEADELDVFMNGVQVC
                     RAGGVGEDRSAVDLSGREVSVVVDLHAGGAEATVWTNDLTHDYVHENSAYST"
     gene            2056..>2886
                     /gene="argB"
                     /locus_tag="VFC06_07905"
     CDS             2056..>2886
                     /gene="argB"
                     /locus_tag="VFC06_07905"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015771397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HZL81839.1"
                     /translation="MISIHNYGEIPAPWKADVLIEALPWLQRFAGTRVVIKYGGHAMV
                     DDNLKRSFASDVQFLRQVGLYPIVVHGGGPQISAMLKRLGISSEFRGGLRVTTPEVRD
                     VVQMVLTGTVQRELVGLINEDRPYAVGLSGGDGGLMTAQLRHAYVEGEELDVGLVGDV
                     ASINPDAVEDLLRAGRIPVISTVAPDASGEILNLNADTAAAALAQSLGAQKLVILTDV
                     PGLYAHWPDTDSLINWIWVSELEKMLPSLDAGMVPKMEACVRAVRGGVAKAHVIDGRQ
                     P"
BASE COUNT          393 a         1051 c         1023 g          419 t
ORIGIN      
        1 acccccacct gcgcgcgctc gcgggccgcg tgctcgtcga gaccaccacg gagaacctct
       61 ccggtgcgga cgtcgtgttc ctcgccctcc cacacggcgc ctccggggcc gtggcagcgg
      121 agctcgaggg cgccgtcgtg atcgactgcg gcgccgactt ccgccttgag gacgccgcgg
      181 actgggaggc cttctacggc agcccccacg cgggctcctg gccgtacggc ttcccggaac
      241 tccgccacgc gggggagggg gccgcccgcg agcaacggga gatcctcgcc cgtgccaccc
      301 ggatcgccgt caccggctgc aatgttgccg cggtgacgct cgccctgcag cccggcgtcg
      361 ggctcgtcga tgcctccggc atcgtcgcca ccctcgccgt cgggtactcc ggggccggca
      421 aggccccgaa gccgcacctc atggccgccg aggcactggg gagtgcggcg ccctacgccg
      481 tgggcggcac gcaccgtcac atcccggaga tcgagcagaa cctgcgcacc gcaggggcgc
      541 tgaacccgcg ggtcgcgttc cagcccatcc tcgtcccgat gtcgcggggc atcctcgcct
      601 cggtgacggc accgctcacc ggtgaccccg ccgaggtgcg tcccgcctgg gaggctgcct
      661 gcgcggggga gcccttcgtg gaactcctgc ccgagggcga atggcccacg acggcggcgg
      721 tggcgggcgc gaacaccgcc cttgtccagg tggcggtgga cgggcacgcc ggccgcgtga
      781 tcgccatgtg cgcactggac aacctcgtga agggcaccgc cggctccgcg atccaggcga
      841 tgaacctcgc tctcggtctc cccgaaacca ctggcctgac caccgtgggg gtggcgccat
      901 gagtgtgacg gcgccgcggg gcttccgcgc ggccggggtc accgccgggc tcaagaagtc
      961 gggtcgcccc gacctcgcac tggtcgtgaa cgacggcccg ctcgacgtgg cagccggcgt
     1021 gttcaccacc aatcgggtgc aggcggcccc ggtgctgtgg agtcgcaccg cggtgggcga
     1081 cgggcgggcg tgcgccgtca tcctcaattc gggcggtgcg aacgcctgca ccggctccgg
     1141 cgggttcttg gacagccaca ccaccgcgga acatgtggcc gcggtcctgg gggtgggtgc
     1201 ggccgacgtg ctcgtgtgct ccaccgggct gatcggggag cggctcgcca tgcccgtgct
     1261 cctcttgggc gtcgacgcgg ccgcggcggc actcggcgtg accggcggcc cggatgcggc
     1321 ggctgctatc tgcaccaccg acaccgtccc caaggaaacc gtccagcgcg gcgagggctg
     1381 gtcgatcggg gggatggcca agggtgccgg catgctcaac ccggcgctgg cgacaatgct
     1441 ggtggtcctc acgaccgatg cggtcctcga cgccgggatg gccgacgccg cgctgcgcca
     1501 cgcggttgag cactccttca accggctgga ctcggacggg tgcatgtcca ccaacgacac
     1561 cgtcctcctg cttgcctccg gcgccgcagg cgcctccccg gacgcggccg tgttcggggc
     1621 agccctggac gcggcctgcg ccgacctcgc gcgccaactc atcgccgacg ccgagggcgc
     1681 gtcccacgac atcgcggtca ccgtccgcgg tgccaccacg gaaggtgccg cgctcgctgt
     1741 cgcccgcgcc gtgacgtcgt cgaacctgtt caaggcggcg gtcttcggga acgaccccaa
     1801 ctggggcagg gtgctggcgg ctgtcggcac ggtcccggtc gaggtggccg cctacgaggc
     1861 ggacgagctg gacgtcttca tgaacggcgt ccaggtgtgc cgcgccggcg gcgtggggga
     1921 ggaccgctcc gccgtcgacc tgagcggccg cgaggtcagc gtcgtcgtcg acctgcacgc
     1981 cggcggggcc gaggcaaccg tctggaccaa cgacctcacc cacgactacg tccacgagaa
     2041 cagcgcgtac tccacatgat ttcgatccac aactacgggg agatccccgc cccctggaag
     2101 gcagacgtcc tcattgaggc gctgccatgg ctgcagcgct tcgcgggcac ccgggtggtc
     2161 atcaagtacg gcggccacgc gatggtcgac gacaacctga agcgctcctt cgcctccgat
     2221 gtccagttcc tgcgccaggt cgggctctac ccgatcgtcg tgcacggtgg tggcccccag
     2281 atcagcgcca tgctgaagcg gctgggcatc tcgtcggagt tccggggggg tctccgcgtg
     2341 accactcccg aggtgcgtga cgtcgtccag atggtcctca cgggtaccgt gcagcgcgaa
     2401 ctggtgggcc tgatcaacga ggaccggccg tacgccgtcg ggctctcggg tggcgacggc
     2461 gggctcatga ccgcccagtt gcgccacgcc tacgtcgagg gcgaggaact cgacgtcggc
     2521 ctcgtcggtg atgtcgcctc gatcaacccg gacgcggttg aggacctgct gcgggccggg
     2581 cgcatcccgg tgatctcgac cgtggccccg gacgccagcg gggagatcct caacctcaat
     2641 gccgacacgg cggcggcggc gctcgcgcag tccctgggcg cccagaagct cgtgatcctc
     2701 acggacgtcc ccggcctcta cgctcattgg ccggacaccg attcgctgat caactggatc
     2761 tgggtgagcg agctcgagaa gatgctcccc tccctcgatg ccgggatggt cccgaagatg
     2821 gaggcctgcg tccgtgccgt gcgcggcggc gttgcgaagg cccacgtgat cgacgggcgc
     2881 cagccg
//
LOCUS       DASRKH010000491         3305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_3421, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000491 DASRKH010000000
VERSION     DASRKH010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3421"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..1033
                     /locus_tag="VFC06_07910"
     CDS             95..1033
                     /locus_tag="VFC06_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZL81840.1"
                     /translation="MSASAETLTIYSGRNENLVGPILEGLEDAVGTPVEVRYAGSSEL
                     AAQLLEEGAATDADIFFSQDAGALGALANAGRFATLPDDVLDLVPERFRDTDGRWVAT
                     SARARVLAYAPALAPEVEQMTGIDQILDEKYRGLIGYAPTNASFQTFVTALRVDRGEE
                     GAREWLERFAALDPQAFDNNPAVLDAVDGGQVAIGLINHYYWYEQVAEKGADAVSARI
                     RFLTSDDPGALVNVAGVGVLDGSDSPAALAAVKYLLSAEVQQYFADETAEYPVVPGVT
                     TTVHDLAPLGEGLVIDLNDLDSLEETLALLDEVGLT"
     gene            1036..2658
                     /locus_tag="VFC06_07915"
     CDS             1036..2658
                     /locus_tag="VFC06_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010079202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HZL81841.1"
                     /translation="MGQRRRTSASGRRAPLVLFAPALAAAMIALVPIAYLLIRTAEAG
                     WAAILEILWRERTLELVLRGLGLAAAVTTACVAIGVALAWLTARTDLPGRRTWAVVAA
                     LPLAMPSFVAAYAWVSVVPGIAGFWGTFLVLTACTYPYVYLPVAAALRRIDPALEEVS
                     RSLGRGPWRTYLTVTARQVRPAVAAGALLVALYSLSDFGTPSLMRYDVFTRVIHTSYQ
                     SSFDRTPAAVLSLVLVAITVTITVAETRSRGRAEQARIGSGAARGLRAVPLGRGTPLA
                     LAVTGGVAAVALGYPAVVLAYWVSRGQSAGVEWDRLVTAGVSTLWVAFLGAVAATLLA
                     VPVGIVAARFPSKGGRWIEHATWSGHALPGIVVALSLVFFGIRWAEPIYQRTPLLVLA
                     YTVLFMPAAIGSVRAAVAQSPRSLGEVARSLGRGPFGVVRDVSLPLAAPGIAAGFALV
                     MLTAMKELPATLLLRPTGMDTLATRLWTETGVGAYAAAAPYAVALVLLAAVPTFLLSR
                     VHVMVSDGAAVARPTGEVAVLELAVARGGVEP"
     gene            2655..>3305
                     /locus_tag="VFC06_07920"
     CDS             2655..>3305
                     /locus_tag="VFC06_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017835054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZL81842.1"
                     /translation="MTRLQVTGLHKAFGRTEVLRGLDLDVPSGAFAAVLGPSGCGKTT
                     LLRVIAGFEDADAGEVRLGERVVVSGRRGVPAERRRVTIVPQEGALFPHLSVAGNVAF
                     GLPRRMRDRRDRVADLLALVGLGDLGRRMPSELSGGQQQRVAVARALAPGPDVVLLDE
                     PFSALDAALRSSVREDVRTALHEANTTAVLVTHDQEEALSTADVVAVVREGRVVQAA"
BASE COUNT          368 a         1263 c         1193 g          481 t
ORIGIN      
        1 gccggcgtaa cggacctcga ccggggtgcc caccgcgtcc tccaggccct ccaggatcgg
       61 gccgacaagg ttctcgttgc gtcccgagta gatcgtgagc gcttccgcgg agacgctcac
      121 gatctactcg ggacgcaacg agaaccttgt cggcccgatc ctggagggcc tggaggacgc
      181 ggtgggcacc ccggtcgagg tccgttacgc cggctccagc gagctcgccg cccagttgct
      241 cgaggagggc gcggccaccg acgccgacat cttcttctcg caggacgccg gggcgctcgg
      301 cgcgctggcc aacgccggcc ggttcgccac cctgcccgac gacgtgctgg acctggtccc
      361 cgagaggttc cgcgacaccg acggccgttg ggtggccacc tcggcccgcg cccgcgttct
      421 cgcctacgct cccgccctgg cccccgaggt ggaacagatg accgggatcg accagatact
      481 cgacgagaag taccgcggcc tcatcggcta cgcgcccacc aacgcctcct tccagacctt
      541 cgtcaccgcg ctgcgggtgg accgcgggga ggagggcgcc cgggagtggc tcgagcggtt
      601 cgcggcgctc gatccccagg ccttcgacaa caaccccgcc gtcctcgacg ccgtcgacgg
      661 cggccaggtc gccatcggcc tgatcaacca ctactactgg tacgagcagg tggcggagaa
      721 gggtgcggac gccgtctccg cccgcatccg cttcctgacc agcgacgacc ccggggcgct
      781 ggtcaacgtc gccggtgtgg gcgtgctgga cgggagcgac tcccccgccg cgctcgcggc
      841 cgtcaagtac ctgctctccg cggaggtgca gcagtacttc gccgacgaga cagccgagta
      901 cccggtggtc ccgggcgtga ccacgacggt gcacgacctg gcgccgctcg gcgaggggct
      961 cgtcatcgac ctgaacgatc tcgattccct cgaggagacc ctcgcgctcc tggacgaggt
     1021 cggactgacc tgacgatggg acagcgccgc cgcaccagcg cctccgggcg ccgcgccccg
     1081 ctggtgctgt tcgcgcccgc cctggcggcc gccatgatcg ccctggtccc catcgcctac
     1141 ctcctgatcc gcacggcgga agcggggtgg gcggcgatcc tggagatcct gtggcgcgag
     1201 cgcaccctcg aactcgtgct ccgcggcctc ggcctggcgg ctgcggtgac gacggcgtgc
     1261 gtcgccatcg gggtcgccct cgcgtggctg accgcccgca cagaccttcc cggccggcgg
     1321 acctgggccg tggtcgcggc cctgccgctc gcgatgccga gcttcgtcgc ggcgtacgcg
     1381 tgggtgtcgg tcgtcccggg catcgccggg ttctggggca cgttcctcgt cctgaccgcc
     1441 tgcacctacc cctacgtgta cctgccggtg gcggcggcgc tccggcggat cgaccctgcc
     1501 ctggaagagg tgtcccggtc gctgggccgc gggccgtggc ggacgtacct caccgtcacg
     1561 gcgcggcagg tccggcccgc cgtcgccgcc ggggcgctgc tcgtggcgct ctacagcctc
     1621 agcgacttcg ggacgccgtc cctgatgcgc tacgacgtct tcacccgggt gatccacacc
     1681 tcctaccagt cctccttcga ccgcaccccg gccgccgtgc tctcgctcgt gctcgtggcc
     1741 atcaccgtca cgatcacggt ggcggagacg cgcagccgcg ggcgggcgga gcaggcgcgc
     1801 atcgggagcg gggcggcgcg cgggctgcgc gcggtgccac tcggccgcgg gaccccgctc
     1861 gcgctcgcgg tgacgggcgg cgtggccgcc gtcgccctcg gctacccggc ggtggtcctg
     1921 gcctactggg tgagccgcgg ccagtcggcg ggcgtggagt gggaccgcct cgtgacggcc
     1981 ggggtctcca cgctctgggt ggcgttcctc ggcgccgtcg cggcaacact cctcgcggtg
     2041 ccggtgggta tcgtcgcggc gcggttcccg agcaagggcg ggcgctggat cgagcacgcg
     2101 acgtggtcgg gccacgccct gccgggcatc gtggtggcgc tctcgctggt gttcttcggt
     2161 atccgctggg cagagccgat ctaccagcgc acccccctcc tcgtcctcgc gtacaccgtg
     2221 ctgttcatgc ccgcggccat cggctcggtg cgcgccgccg tggcgcagag cccgcgcagc
     2281 ctgggggagg tcgcacgctc gctggggcgg gggccgttcg gcgtggtgcg cgacgtctcc
     2341 ctccccctcg ccgcgcccgg catcgccgcc ggcttcgccc tcgtgatgct caccgcgatg
     2401 aaggagctgc ccgccaccct cctgctgcgg cccaccggca tggacaccct cgccacccgg
     2461 ctgtggaccg agacaggggt gggggcgtac gcggcggccg cgccctacgc cgtcgcgctc
     2521 gtcctgctgg cggcggtgcc cacgttcctg ctctcgcgcg tccacgtcat ggtgtccgac
     2581 ggcgccgcgg tcgcgagacc aaccggtgag gtggccgtcc tcgaactggc cgtggcacga
     2641 ggaggggtgg aaccgtgacc cgcctgcagg tgaccgggct gcacaaggcc ttcgggcgca
     2701 ccgaggtgct gcgcggcctc gacctcgacg tgccctccgg cgccttcgcc gccgtcctgg
     2761 ggccgtccgg ctgcggcaag acgaccctcc tgcgcgtgat cgcgggcttc gaggacgccg
     2821 acgccggcga ggtccgcctc ggggagcgcg tggtggtctc cggccggcgc ggggtgccgg
     2881 ccgagcggcg ccgggtgacg atcgtgccgc aggagggggc gctcttcccg cacctctcgg
     2941 tggccggcaa cgtggcgttc ggcctgcccc ggcgcatgcg cgaccgccgc gaccgggtcg
     3001 ccgacctcct cgccctcgtg ggcctcggcg acctcgggcg ccgcatgccg tcggagctct
     3061 ccgggggcca gcagcaacgc gtggccgtgg cgcgggcgct cgcccccggg cccgacgtcg
     3121 tcctgctgga cgagcccttc tccgccctcg acgccgccct gcgctcctcg gtgcgcgagg
     3181 acgtgcgcac cgccctgcac gaggcgaaca ccaccgcggt cctcgtcacg cacgaccagg
     3241 aggaggcgct cagcaccgcc gacgtcgtcg cggtggtgcg cgaggggcgg gtggtccagg
     3301 cggcc
//
LOCUS       DASRKH010000492         3272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Demequina sp. isolate SMAG_U10157 k141_12598, whole
            genome shotgun sequence.
ACCESSION   DASRKH010000492 DASRKH010000000
VERSION     DASRKH010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963685
            Sequence Read Archive: SRR5660398
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequina sp. (soil metagenome)
  ORGANISM  Demequina sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae; Demequina.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:09:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,587
            CDSs (total)                      :: 1,567
            Genes (coding)                    :: 1,526
            CDSs (with protein)               :: 1,526
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 7 of 41
            Pseudo Genes (incomplete)         :: 36 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Demequina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12598"
                     /isolate="SMAG_U10157"
                     /isolation_source="soil"
                     /db_xref="taxon:2050685"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..677)
                     /locus_tag="VFC06_07925"
                     /pseudo
     CDS             complement(144..677)
                     /locus_tag="VFC06_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867767.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(866..2419)
                     /gene="mmsA"
                     /locus_tag="VFC06_07930"
     CDS             complement(866..2419)
                     /gene="mmsA"
                     /locus_tag="VFC06_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HZL81843.1"
                     /translation="MQSITKEFPGVKALDEVTLTVEQGEIHAICGENGAGKSTLMKVL
                     SGVYPYGTYTGDIVFEGEVARFHSINDSEHAGIVIIHQELALVPHLTVAENIFLGNER
                     RGRGGLIDWNRANHEATQLMNQVGLHENPMTPVSQLGVGKQQLIEIAKALSKEVKLLI
                     LDEPTAALNDRDSAHLLDLLRTLKERGITSIMISHKLHEIESVAETTTVIRDGKTIEA
                     LDMADPDANQDRLIRGMVGRDLEHRYPEHDHHIGEEVFRVENWTVYHPTQQERVIIDN
                     ASFFVRAGEVVGLAGLMGAGRTELAMSLFGRAYGHGHSGSIYKNGKQLHLKTVSDAIS
                     AGIAYATEDRKKYGLNLIDNVRRNISAAALFKLTKNGFVNGNEELKVAEEYRSSMNIK
                     TPTVMQTINNLSGGNQQKVVLSKWIYTDADVVLLDEPTRGIDVGAKYEIFTIVNRLAA
                     EGKAVLVISSELPELLGTCDRIYTLAFGRITGVLDVADATQEGLMKLMTQERSAANAG
                     TEEMEQAKS"
     gene            complement(2537..>3272)
                     /locus_tag="VFC06_07935"
     CDS             complement(2537..>3272)
                     /locus_tag="VFC06_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZL81844.1"
                     /translation="FYVTFDNYQVGVQQATSLLVGLGLLNQDGSEGTATGPFNVELFA
                     GSLDDNNAFFFWNGAIDTLQPYLDAGTLVVPSGQMDIEQAAILRWQQETAQKRMEDLL
                     TASYSGGDLKVNGVLSPYDGLSRGIITALQNAGYPATIAEGFPVVTGQDAEIASVKLI
                     ADDVQSASIFKDTRKLAAQAVIAVGEYLSGGEPTANDTTTYDNGVKVVPSYLLESDIV
                     YTDNIQALLVDSGYYTADEVASGVAS"
BASE COUNT          572 a          968 c         1047 g          685 t
ORIGIN      
        1 tacaccgatg ccgatgtggt gttcctcgat gagccgaccc gcggtattga cgtgggcgcc
       61 aagttcgaga tcttcacgat cgtgaacagg ctcgcggcgg agggcaaggc ggtcatcgtg
      121 atctcgtccg agttgccctc cgccgcgagc ctgttcacga tcgtgaagat ctcgaacttg
      181 gcgcccacgt caataccgcg ggtcggctca tcgaggaaca ccacatcggc atcggtgtag
      241 atccacttgg acagcactac cttctgctgg ttgccgcccg agagtgtgtt gaccgtctgc
      301 atcaccgtcg gggtcttgat attcatcgac gagcgatact cctcggcgac cttgagttcc
      361 tcgttgccat tcacaaggcc gttcttggaa agcttgaaca gggccgcagc cgagacgttg
      421 cgcttcacgt tgtcgatcag gttgaggccg tacttcttgc ggtcctcggt cgcgtaggcg
      481 atacccgcgc tgatcgcgtc cgacacagtc ttgaggtgca gctctttgcc gttcttgtag
      541 atcgatcccg agtgcccacg gccgtaggcg cgcccgaaga ggctcatggc cagttcggtc
      601 cggcccgcgc ccatgaggcc agcaatgccg accacctcgc ccgccctgac aaagaaggat
      661 gcgttgtcga tgatgacgat caccatgccg atggcgagca ccaggatgta cgaatactgg
      721 agcacgatgt tggtgaggtt tccgggactc agcagcgtcc cgtcggtcaa ctgcgtgaac
      781 agcaacacga tcgcgacgaa tgcgatgaag attccgctct ggcgcaggtt gcgcaaaaca
      841 acgtctctga ggtgggcggt gcctgtcatg acttggcctg ttccatttcc tcggtgcctg
      901 cattggcggc cgaacgttct tgggtcatga gtttcatcaa gccctcttgg gtggcgtcgg
      961 cgacgtcgag gactccggtg atgcgcccga aggcgagggt gtagatccgg tcgcacgtgc
     1021 ccagcagttc gggcaactcg gacgagatca cgaggaccgc cttgccctcc gccgcgagcc
     1081 tattcacaat cgtaaagatc tcgtacttag cgcccacgtc aataccgcgg gtcggctcat
     1141 cgaggagcac cacatcagca tcggtgtaga tccacttgga cagcaccacc ttctgctggt
     1201 tgccgcccga gaggttgttg atcgtctgca tgaccgtcgg agtcttgatg ttcatcgacg
     1261 agcggtattc ctcggcgacc ttgagctctt cgttgccatt cacgaagccg ttcttggtga
     1321 gcttgaagag cgcagctgcc gagatgttgc ggcgcacgtt gtcgatgagg ttgagcccgt
     1381 acttcttgcg gtcctcggtc gcgtaggcga tgcccgcgct gatcgcgtca gacaccgtct
     1441 tgaggtgcag ctgtttgccg ttcttgtaga tcgatcccga gtgcccatgg ccgtaggcgc
     1501 gcccgaacag gctcatggcc agttccgtgc ggccggcacc catcaggcca gcaaggccga
     1561 ccacctcgcc agccctgaca aagaaggagg cgttatcgat gatgacgcgt tcctgctggg
     1621 tggggtggta cacggtccag ttctccacac ggaaaacctc ttcgccgatg tggtggtcgt
     1681 gctcggggta ccggtgttcg aggtctcgac ccaccatgcc gcggatgagg cggtcttggt
     1741 tagcatcggg gtcggccata tcgagcgcct cgatggtctt gccgtcgcgg atcaccgtgg
     1801 tggtttccgc gacggactcg atctcgtgga gcttgtggga aatcatgatc gacgtgatgc
     1861 cgcgctcctt gagggtgcgc aacaggtcga gcaagtgtgc tgaatcgcga tcgttgagcg
     1921 ctgcggtggg ctcgtcaagg atgaggagct tcacctcctt cgagagcgcc ttcgcgatct
     1981 cgatgagctg ctgcttaccg acgccgagct ggctgacggg agtcatgggg ttctcgtgta
     2041 gcccgacctg gttcatcagc tgggtggctt cgtggttcgc gcggttccag tcgatgaggc
     2101 cgccgcggcc acggcgttcg ttgccgagga agatgttctc cgccaccgtg aggtggggca
     2161 ccaaggcgag ctcctggtga atgatgacga tgcctgcgtg ctcggagtca ttgatcgagt
     2221 ggaagcgggc aacctcgccc tcaaagacga tgtcccctgt gtaggtgccg tacgggtaca
     2281 cgcccgacag caccttcatg agtgtcgact tgcctgcgcc gttctcgccg cagatggcgt
     2341 gaatctcgcc ttgctcgaca gtcagggtca cctcgtccag cgccttgacg ccggggaact
     2401 ccttggtgat gctctgcatc tgaaggatcg tggacacgcg cctacctccg ttggtcggtc
     2461 gtggtgtggg gccgaccccc aggtggtgcc gggcggatta cgcccggcac caccgtgggt
     2521 tcggcgggag tactgactag ctggctacgc cggaagcgac ctcatcggcc gtgtagtatc
     2581 cggagtcaac cagaagtgcc tggatgttgt cggtgtacac gatgtccgac tcgagcaggt
     2641 acgagggaac gaccttgacg ccgttgtcgt acgtggtcgt gtcattcgcc gtgggctcgc
     2701 caccgctgag gtactcgcca actgcaatca ctgcctgagc agccagcttg cgggtgtcct
     2761 tgaagatcga cgcagactgc acgtcgtccg caatgagctt gaccgaagcg atctcagcgt
     2821 cctggccggt gacgaccggg aagccttcag cgatcgtggc cgggtagcca gcgttctgca
     2881 gggcggtgat gatgccgcgc gagagaccgt cgtaaggcga cagcacgccg ttgaccttga
     2941 ggtcaccacc ggagtacgac gccgtcagca ggtcttccat acgcttctgc gcggtctcct
     3001 gctgccagcg caggatggct gcctgctcga tgtccatctg accggaagga accacgaggg
     3061 tgcctgcgtc caggtagggc tgcagggtgt cgatggcacc gttccagaag aagaacgcgt
     3121 tgttgtcatc gagcgaacct gcgaagagct ccacgttgaa cggaccggtg gcagtgccct
     3181 cggaaccgtc ctggttgagc aggcccaggc cgacgagcag cgacgtagcc tgctgcacac
     3241 ccacctggta gttgtcgaac gtcacgtaga ag
//
